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<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYM5</accession>
    <name>A0A6I9WYM5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 68-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106537593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907251.1">
        <property type="nucleotide sequence ID" value="XM_014051776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYM5"/>
    <dbReference type="GeneID" id="106537593"/>
    <dbReference type="KEGG" id="tsr:106537593"/>
    <dbReference type="OrthoDB" id="44277at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753:SF22">
        <property type="entry name" value="PHOSPHOLIPID_GLYCEROL ACYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="1">
        <location>
            <begin position="325"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907251.1"/>
        </source>
    </evidence>
    <sequence length="544" mass="62302" checksum="0F6267C69959FD3B" modified="2020-10-07" version="1">MSDTDPVIVHALAPSSVFAFRSEAESWDAFLERFEVFLQAHDLSELPEGRRRGTFLHYCGPEMFATARALSAPTPVHTMPLDLLMARLKLHYELTPSKYSRRQFFRRCIQRQSISQYVAELRAAAINCEFSDLEDALLEQFIYGLLDINLHRQILSRTELSMKIAMDEAWTYKMASQSTPDVRRFPPSALLALPNSAVHSQMMVLDPCADATASVDCLRSMPPRDGDVKDQPSLFGALWFPRHFCTLHPWSLRLFWIETLIVPVCLFVCLFVEVPWDQRWKAKEAAITSRTHKLNGNYSSDIWDPVRLIIAIILDGYGRLWHGYELHGIENIPDGPGLIIYYHGAVPMDYLCFLAKIFILKRKCCYSIADDCVFIFPGVQALAGVTGIIQNKKEECLNVLKKGKLLGISPGGMREALFSDKSYKLIWHKRKGFAQLAVDMKVPIIPMCTQNIREGYWIFGRSRALARRLYEYSRIPFLPVYGGFPVKFRTYIGEPILYNPNVTSEELAEKTQIALQSLIRKHQQIPGSICRALLERFHKEKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZM1</accession>
    <name>A0A6I9WZM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106537834</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013907571.1">
        <property type="nucleotide sequence ID" value="XM_014052096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZM1"/>
    <dbReference type="GeneID" id="106537834"/>
    <dbReference type="KEGG" id="tsr:106537834"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000085">
        <property type="entry name" value="Putative ras-related protein rab-11a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013907571.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24469" checksum="909F1E43DAAC549F" modified="2020-10-07" version="1">MGGSRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKMQCCQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZM7</accession>
    <name>A0A6I9WZM7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ras-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RASIP1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908429.1">
        <property type="nucleotide sequence ID" value="XM_014052954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZM7"/>
    <dbReference type="GeneID" id="106538467"/>
    <dbReference type="KEGG" id="tsr:106538467"/>
    <dbReference type="CTD" id="54922"/>
    <dbReference type="OrthoDB" id="3908708at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22734">
        <property type="entry name" value="FHA_RAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15472">
        <property type="entry name" value="Myo5p-like_CBD_Rasip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037983">
        <property type="entry name" value="CBD_Rasip1/Radil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF4">
        <property type="entry name" value="RAS-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="2">
        <location>
            <begin position="290"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908429.1"/>
        </source>
    </evidence>
    <sequence length="656" mass="73853" checksum="9D96AF99D63BBA27" modified="2020-10-07" version="1">MNLSTKRRKDRKGVLSMAVGEPVEGGSVEESRLKAAEEAEGNPAPSASEEEVTDVASLEQLTQCLIQPPTEHPYFLQLLGYNEKQEFVIHVMTRRRHIFGRPERRPAPDQSSFVDTFLNAPDILPRHCCVVSSVPPADPSQPWSSAMVRPFRGASITHNGAPLHRQVELAPGDLLGLGQYFLFMYKDPRAATAPPAWLPKAWVSPGLVGALACQACGRSLQERQEAFRAYLKSREPQLRYRPQEQEALLAEIVRLQETSTCRLGPAFLFGLCLEHAARELEPGHLPRLIARIAQLVKEAVWGKIKEIRDRQPENQQDKGEAGNLSIEEVAADLRPLMLWMANSMELLNLVQKRVLDIEKDLDLEGVSHNALLSSDLETCDEAMAVLDEVIMSTFQQSVYYLTKTLYSALPVLLDSNPFTATTDLLHVPELSSMPEGIQGTLAIYQATLELTRDCDLHPDLVSQTFGYLFFFSNASLFNTIMERGGSQSLFQWAKAVQIRTNLDLVLDWLQRIGLGDIATEFFRKLSNTANLLCVPKSSLLQTSWSRLRNDYPALTPAQLQHVLRNYQLGPGRASPECWNPPDKEQDEMTQSDIFESFTDHPPLILPCDGFRLRLSEPVLDDTLHRQLVRLRRFLWELERESLPANQRAVYGLDPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZY9</accession>
    <name>A0A6I9WZY9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SAP30-binding protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SAP30BP</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907067.1">
        <property type="nucleotide sequence ID" value="XM_014051592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZY9"/>
    <dbReference type="GeneID" id="106537457"/>
    <dbReference type="KEGG" id="tsr:106537457"/>
    <dbReference type="CTD" id="29115"/>
    <dbReference type="OrthoDB" id="1714508at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012479">
        <property type="entry name" value="SAP30BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464:SF0">
        <property type="entry name" value="SAP30-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07818">
        <property type="entry name" value="HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907067.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="34116" checksum="69FFEF8484D17B29" modified="2020-10-07" version="1">MAAAAKKSVLSSLAVYAEDSEPESDSEAGGAGSDRGGGTEEKAGLVSESYGEDDLSKMEGEEDGYDEDDDENSRQSEDDDSETEKPEADDLKDFSELEKRDPQELVASFSERVRNMLPDEIKIPPEPQGRCSNHLQDKIQKLYERKMKEGMDMNLIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWAEDSYYEALAKAQKIEMDKLEKAKKERTKIEFVTGTKKGTTNAPSTTTTTASTAGGDAQKRKSKWDSAIPVTTIAQPTILTTTATLPAVVTVTTSASGSKTTVISAVGTIVKKAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0K6</accession>
    <name>A0A6I9X0K6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MHC class II transactivator-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106537644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907312.1">
        <property type="nucleotide sequence ID" value="XM_014051837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0K6"/>
    <dbReference type="GeneID" id="106537644"/>
    <dbReference type="KEGG" id="tsr:106537644"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907312.1"/>
        </source>
    </evidence>
    <sequence length="235" mass="25328" checksum="1E24A1AE07A68C25" modified="2020-10-07" version="1">MDLFKQILPEVREVLLTASAPQLQTFLDSMLSEGILSREYYQTLLDEKDTDDLARKLALTLLGKRAGPSDPHSRLPPLQLSGNARGPAKNGAVSLRTRNWTAAPAPDEVSPGGIPPETPCLQSEAHPASMDEMEEAPAVQVSEAASDDQDNPEVFYTVERDESGEVLSLCADMGEAYDKIGILSRAVGLYVPNFVKVKGEGNVGASPRIGGCIKRCMNEGMISLLQLAKRFGGIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X234</accession>
    <name>A0A6I9X234_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZDHHC21</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013908386.1">
        <property type="nucleotide sequence ID" value="XM_014052911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X234"/>
    <dbReference type="GeneID" id="106538429"/>
    <dbReference type="KEGG" id="tsr:106538429"/>
    <dbReference type="CTD" id="340481"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF11">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="86"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013908386.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="31381" checksum="F6171AE9C42CDFD1" modified="2020-10-07" version="1">MGLRIHFVVDPQGWFCMGLIIFVWLYNILFIPKIILFPHYEEGHISAAAILCYYLFSLFCIASLLRASVADPGRLPESPKIPITEKDSWELCNKCNMMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLSSYTLLLDFCHYYYFEPLRKVNADVFVFRHELALTRISTFMGIIMLVGITGLFYTQLIGIFNDITSIEKLANCCEEISRPRKPWQQTFSEVFGTRWKILWFIPFRQRRPLRVPYHFANHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2V8</accession>
    <name>A0A6I9X2V8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein 572-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106539354</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909614.1">
        <property type="nucleotide sequence ID" value="XM_014054139.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539354"/>
    <dbReference type="KEGG" id="tsr:106539354"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000180">
        <property type="entry name" value="Zinc finger protein 689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF114">
        <property type="entry name" value="KLUMPFUSS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013909614.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28940" checksum="D2B292BF941EB7E2" modified="2020-10-07" version="1">MHDRIHTGEKPYKFSECGQTFGQNKSILFHGRTHAGENLYNGSQCSKSFSIHGNVIHQRIHTGEQPFECPDCGKSFSWNSHLVAHHRTHTGEKPFECPDCGRSFSWNSSLVLHQRTHTGEKLFECPDCGKYFSQNSNLVKHQRTHTGEKPFECPDCGKSFTKNYRLVRHQRTHTGEKPFECPACGKSFSHNSSLVEHQRTHTGEKPFECPDCGNSFSKNSNLINHQRIHTGDKPFKCPDCGKRFSQNSXPVRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3V8</accession>
    <name>A0A6I9X3V8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Collagen alpha-1(XXVIII) chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">COL28A1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">May act as a cell-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the VWA-containing collagen family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the venom Kunitz-type family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909984.1">
        <property type="nucleotide sequence ID" value="XM_014054509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3V8"/>
    <dbReference type="GeneID" id="106539668"/>
    <dbReference type="KEGG" id="tsr:106539668"/>
    <dbReference type="CTD" id="340267"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000051">
        <property type="entry name" value="Collagen type XXVIII alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1112">
        <property type="entry name" value="COL_CUTICLE_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11 21" id="KW-0176">Collagen</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026794548" description="Collagen alpha-1(XXVIII) chain" evidence="17">
        <location>
            <begin position="24"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="49"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="798"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="19">
        <location>
            <begin position="1095"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="241"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="258"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="715"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="737"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013909984.1"/>
        </source>
    </evidence>
    <sequence length="1148" mass="120140" checksum="058B8649A1AF3E2A" modified="2020-10-07" version="1">MWKKHSVLYLLVLAIAINNVVYGQNRRKGQRLSPLARKDNLQDSVCFIDIIFVLDSSESAKGILFDKQKEFAILLSDKVFLMKPTRALRYDIRLAIMQFSSTVRIDYPFSVWRNLQIFKQKVNEMSYIGHGTYSYYAISNATQLLKAEGRKTGIKVVLLMTDGIDHPKSPDIQVISEKARALGISFITIGLSNDVNQTKLHLISGDSPGKSVFILNEPNLSDKIRNQLATLFEERCGKSCECQKGDQGPPGPPGGHGGRGDKGEQGPKGQKGEAEKGEPGEKGSEGNPGYKGEKGDRGECGTPGLKGDRGLDGPSGPKGPRGPQGIGGPPGDSGSKGIQGNKGEPGPAGPYGPAGIPGIGYPGPKGDRGQEGRIGLPGPTGIGEPGLTGPRGPEGMQGERGPPGEGLPGRKGDKGSEGPAGPVGSPGLSIKGDKGERGIEGVQGPVGPPGTGIQGNQGIQGLKGAPGVKGSRGIGLPGQKGEMGPSGQKGDIGAPGIGQPGPKGDPGRSGFTGESGKPGIQGIPGKKGEQGLPGPRGPDGIPGTGHLGPKGEEGQKGAQGLDGPRGIPGPAGPKGESGIKGDIGSPGPSIRGLPGPKGEQGYPGPPGNDGSPGHTIIGPKGAPGVIGPPGPQGAKGDGFPGPPGSQGLPGFPGPRGLKGAGDPGQKGEQGARGPPGPPGQRGVGIPGPKGIMGQKGFPGPPGAPGDSIQGNKGEQGIQGSSGPKGSIGIGLPGPKGDYGEKGEQGKRGNKGEIGEPGPQGPRGFSGQKGEPGLTKEEIIKLIMEICGCGVKCKENPLELVFVIDSSESVGPENFERIKRFVKTMIDAITVNQATTRIGIINFSLKVEVVSTLQQFPTKDSLKNAVDTMQYYGEGTYTATAISKAIDVFQMARQGVRKVAVVITDGQADQRDPHKLEVVVRDAHALKIEIFVIGVVQKTDPNFENFRKEMDLIATDPDSDHVYQIDDFMTLQALEDKIFKQICENDLSSYITEVLSSALSPRFETSEHGTQLAKIDIPGSYAPPLHPETSDSFSKKPSEKEALYLDRYHNVTPTTYKYIPEHHRRPVTPHPDLAVVPTPAVVLLPTVIHVMKETRCLEPMKTGSCRNYQVKWFYAKDTNSCARFWYGGCEGNQNRFETEQECQAICVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4A1</accession>
    <name>A0A6I9X4A1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc finger CCHC domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ZCCHC2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013910164.1">
        <property type="nucleotide sequence ID" value="XM_014054689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4A1"/>
    <dbReference type="GeneID" id="106539797"/>
    <dbReference type="KEGG" id="tsr:106539797"/>
    <dbReference type="CTD" id="54877"/>
    <dbReference type="OrthoDB" id="6361509at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042793">
        <property type="entry name" value="ZCCHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46939">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46939:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="833"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="660"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="857"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013910164.1"/>
        </source>
    </evidence>
    <sequence length="879" mass="95989" checksum="62293A745D081E52" modified="2020-10-07" version="1">MASLHPAFSFHQRVTFREHLERLRGALCRGEDENFSHNCMPTSESSLLEQRAGVHDKLMVATHRPQQEAVHIQKIMLKGVPRKRSDKHLEYTFKVIWSDHSVSSVTKTHQELQEFLLKLPKDLSSESFDKTILAALNQGLQKQEERRHSDFEPMIRQLFANTSPAFLQNQRVNNFFQTLSSEDLHSHNNHQSSLKTSKMPEHFKEDSSEASSQEEDMMQHPVIHKKHNGKCPIANTINTRCPSLDGFDVAHGEYNGVAVADWRRKGWALQQHPEHCTILDQHAGEKKNLPVLNKKKGKQQSEKDKIKRTDSRLTMRANYVRVADAHHLHPGTVKDSNLDITIGNDTCGETSSESYSSPSSPRHDGRESFESEEEKDRDTDSNSEDSGNQTVLRFTEYGATDFNKALTQMSVTSNNKNTVEDPLSSQFQHISFMPALHCVVLNSAQKPETVVPPPISADGKTLGMVVANSAPVGMGSSVESEKHIELMSSPLPATFFPSTVQPGSPALQLGMHRMKISSPQASENCGVNVPQQQTGSLNIGSPNTAFIPVHSPGNFTGTPLPTADPVPKSVSHVMGLNQMVPHVDGNTGVIPQPPNLKVVVPATGLSTALPPAPFTVPVTLHATNVLPNQSTTVLNTVASTPPAPTTGLCVSQVQPTIPPAIPTHTPGPAPSPSPALTHSTAQSDSTSYINAVGNTNANGTLLSPQQQQQQQLGPCGSCGRRCSCGANGNNLQINSYFYPNQIQGQVYRVSPFFHLPFCNGSYLSQGHQANGTQIPYFFSRGPYSMHEPVMGSQINYGVQQMAGYRGFYPVYQASNVVASSNGSGPKKTGNVSCYNCGVTGHYAQDCKQSSMEANQQGTYRLRYAPPPPPPSNDTLDSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4J4</accession>
    <name>A0A6I9X4J4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Periodic tryptophan protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PWP1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013909211.1">
        <property type="nucleotide sequence ID" value="XM_014053736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4J4"/>
    <dbReference type="GeneID" id="106539059"/>
    <dbReference type="KEGG" id="tsr:106539059"/>
    <dbReference type="CTD" id="11137"/>
    <dbReference type="OrthoDB" id="270624at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001779">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001617">
        <property type="entry name" value="WD40-repeat-containing domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="247"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="290"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013909211.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="55132" checksum="697E9041687DED49" modified="2020-10-07" version="1">MSRPVTCVAWVPQGVAKETPDKVQLDKEELKRLITEAKERLQTGDDDDDDESGQEGTSGAMDDVDGEVASSSPKDEDTDKLDDDELAEYDLDNYDEENAGTEGLGEIFAGLAVYGTNENDPYITIKDTDQYEKEDFVIKSNDSLVICGRVDKDHCSLEVHVYNHEEDSFYVHHDLILSAYPLSVEWMNFDPSSNDSAGNYIAVGTMKPVIEIWDLDIVDSLEPVFSLGHKAKLKKKKQKKNLSKEDHQGHTDAVLDLSWNKNLRSVLASASADHSVILWDMSRGTTAATLSLHTDKVQTLQFHPFETQTLISGSYDKSAILYDCRSPQDNHRTWRFSGQVERVTWNHFSPHHFLASTEDGFVYCLDARSHKPVFTMKAHDEEVSGLQLSSQIKGCLVTTSSDKSVKIWDILGNKPSLVYSRDMKMGSLFCAGCCPDLPFVYVFGGERQGLQLWDISTISAVNDVFGTRQRLAVTTSSSEATNSANSSSSCGGAAMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6U0</accession>
    <name>A0A6I9X6U0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cullin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106540539</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911174.1">
        <property type="nucleotide sequence ID" value="XM_014055699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6U0"/>
    <dbReference type="GeneID" id="106540539"/>
    <dbReference type="KEGG" id="tsr:106540539"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000016">
        <property type="entry name" value="Cullin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013911174.1"/>
        </source>
    </evidence>
    <sequence length="169" mass="20188" checksum="4978B62F46E925DD" modified="2020-10-07" version="1">MSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYMETKFFLENHVRHLHKTVLDSEEKVLVMYYRNWDEYSKGAEYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMSEPLMEIGELALDMWRRLMIEPLQAALIRMLLCEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7R3</accession>
    <name>A0A6I9X7R3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger transcription factor Trps1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16 17" type="primary">TRPS1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909887.1">
        <property type="nucleotide sequence ID" value="XM_014054412.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909893.1">
        <property type="nucleotide sequence ID" value="XM_014054418.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909901.1">
        <property type="nucleotide sequence ID" value="XM_014054426.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539598"/>
    <dbReference type="KEGG" id="tsr:106539598"/>
    <dbReference type="CTD" id="7227"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001213">
        <property type="entry name" value="Transcriptional repressor GATA binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000020">
        <property type="entry name" value="Zinc finger transcription factor Trps1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028440">
        <property type="entry name" value="TRPS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47034">
        <property type="entry name" value="ZINC FINGER TRANSCRIPTION FACTOR TRPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47034:SF1">
        <property type="entry name" value="ZINC FINGER TRANSCRIPTION FACTOR TRPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="13">
        <location>
            <begin position="902"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="15"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="376"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="468"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="778"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="862"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="974"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1051"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1185"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="496"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="778"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="974"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="992"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1057"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013909893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909901.1"/>
        </source>
    </evidence>
    <sequence length="1293" mass="143244" checksum="3A55AAE4167BCBC8" modified="2020-10-07" version="1">MPYEVNAGYDFTNMVRKKNPPLRNVVREGETETLQSPGTESDNTERKEEISAGQMQDNTELGEAVEQNDENFCTHVPEPSPSSKKDLKSSTTSEKAGFNYESHNKGGNVPSFPHDEMTDRNMPAFSSPAVGGISESLKSPLRSDADDTQDVACNLSVDLTEKSEETRTSPKSMDETMHVQSGQAEGRGSPASLASKNPQVPSDGDLRPIKLKADLSATENPDTAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDTKILALHNMVQFSHSKDFQKVNCSVLSGVLQDISSQRPVLLNGTYDVQVTLGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGNSSTELEQHFLQTHPNKIKASLPVSEGSKATERNSNKSSPTVRSGEMADLAKWQDKITIRCGDDTPVGYSVPIKPLESSRQNGTDASSYYWCKFCSFSCESSSSLKLLEHYSKHHGGSQAGGPHPNRNDKLSRGSVINHNDITKNADGELATKSEKGSSMAKKKDFSSSGVEDHVVTSYNCQFCDFRYSKSHGPNVIVVGPLLRHYQQLHNIHKCTIKHCQFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGMPGGTRVKHQCHQCSFSTPDVDVLLLHYETTHEAQVSEVKQEANALQGGDGPLTVKESKEHSCTKCDFVTQVEEEISRHYRRVHNCYKCRQCSFTALDTQSLLEHFNTVHCQEMDITTANGEDSHGMMAALKEESKIDLKVYNLLSSDPKMGEPILESVVKKEKMEEKEGSKEKGWPDGSSDDLRGTTWRGVDILRGSPSYTQTSLGLLTTVSVVQEQPKALRDSPNVEAAHLARPVYSLPVETKGFLQGMPQGSGEKSGPLSQPYPGSADNKSKDESQSLLRRRRGSGVFCANCLTTKTSLWRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPEALQAEQLNKQQRGNSEEQVNGSPLDRRSEDHLTEGHQREIQLPSLSKYEAQGSLTKSHSAPQSILVGQSLDIHKRMQPLHIQIKSPQESSGDPGNSSSISEGKGSSERGSPIEKYMRPAKHLNYSPPGSPIEKYQYPLFGLPFVHNEFQSEADWLRFWSKYKLSVPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSRPGATANGASKEKAKTASNVKNEGSLNVVKTEKVDRSTQDDLSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSICQYFCTDKYDFTTHIQRGLHKNNAQAETNGKPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8J4</accession>
    <name>A0A6I9X8J4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">XK-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">XKR7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the XK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911859.1">
        <property type="nucleotide sequence ID" value="XM_014056384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8J4"/>
    <dbReference type="GeneID" id="106541056"/>
    <dbReference type="KEGG" id="tsr:106541056"/>
    <dbReference type="CTD" id="343702"/>
    <dbReference type="OrthoDB" id="6356248at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902742">
        <property type="term" value="P:apoptotic process involved in development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043652">
        <property type="term" value="P:engulfment of apoptotic cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070782">
        <property type="term" value="P:phosphatidylserine exposure on apoptotic cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018629">
        <property type="entry name" value="XK-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050895">
        <property type="entry name" value="XK-related_scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024:SF7">
        <property type="entry name" value="XK-RELATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09815">
        <property type="entry name" value="XK-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU910716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013911859.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36947" checksum="1CA9AAEAAB8E5E14" modified="2020-10-07" version="1">LRSSREGLSASASLVSLAWTLASYQKVLRDSREDKMPMSYKGAMVQVLWHLFTIAARAIAFALFASVFQLYFGIFIVTHWCIMTFWIIQGETDFCMSKWEEIIYNMVVGIIYIFCWFNVKEGRSRCRMSIYYVITLLENAALTGLWYWFREQYVISEFYALIIVCVVASSFALGVFFMFIYYCLLHPSGTIFGAQNTSCLCGEESTAVPRATGPPPDVITSPPRSLPRTTEREGIPGDRDSCGPVFQVRPSMPSTPIARAPRTEGPVIRIDLPRKKYPAWDAHFIDRRLRKTILALEYTSPATPRLQYRSMGASQELLEYETTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8Q6</accession>
    <name>A0A6I9X8Q6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Reticulocalbin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RCN1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CREC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910132.1">
        <property type="nucleotide sequence ID" value="XM_014054657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8Q6"/>
    <dbReference type="GeneID" id="106539773"/>
    <dbReference type="KEGG" id="tsr:106539773"/>
    <dbReference type="CTD" id="5954"/>
    <dbReference type="OrthoDB" id="293868at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16229">
        <property type="entry name" value="EFh_CREC_RCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000109">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000110">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000213">
        <property type="entry name" value="Reticulocalbin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027241">
        <property type="entry name" value="Rcn1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF17">
        <property type="entry name" value="RETICULOCALBIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026945673" evidence="8">
        <location>
            <begin position="26"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="111"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="162"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="199"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013910132.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="38736" checksum="39EB87F7201F4C1C" modified="2020-10-07" version="1">MSLGTTSRSLTVGLMLLLCINRSLGVPTFRKERVVRLDPELGNRQHEENQSFQYDHEAFLGKDDAKTFDQLSPQESQERLGKIVDRIDDDKDGFITTEELKNWIKRVQKRYIFENVAKVWKDYDLNKDNKISWEEYKQASYGYYLEHSKEFQDVTEQHNFKKMLPRDERRFKQADLDGDSEATREEFTAFLHPEEFEHMKDIVVLETLEDIDKNEDGFVDQDEYIADMFAHEDGGPEPDWVVTEREQFADFRDLNKDGKMDKEEIRHWILPKDYDHAQAEARHLVYESDADKDQKLTKQEILDNWNMFVGSQATNYGEDLTKNHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8X3</accession>
    <name>A0A6I9X8X3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch repeat and BTB domain-containing protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KBTBD12</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910197.1">
        <property type="nucleotide sequence ID" value="XM_014054722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8X3"/>
    <dbReference type="GeneID" id="106539825"/>
    <dbReference type="KEGG" id="tsr:106539825"/>
    <dbReference type="CTD" id="166348"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18485">
        <property type="entry name" value="BACK_KBTBD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF491">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="31"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013910197.1"/>
        </source>
    </evidence>
    <sequence length="619" mass="70666" checksum="0E46CE83370C4334" modified="2020-10-07" version="1">MDYKAVGKRKQQHSLNLLDQIKYMKDLTEMIDVVLVAEEKKFPCHKLILAAFSPYFKAMFTCGLMECAQREVVLHDIAAESVSVLLHYMYTSELHLTNCNVQNVAIAAFFTQMEDVFSQCQTYMMDHIEASNSIGIYYFAKHIGAEELSERARKYLYQHFTEVSQQEEILEVEAQQLLSLIRSDDLNVSREESILDLVLRWVNHSRKLRADHLIELLKQVRLVLVSPSFLVEARKRNTVLLSNSKCFEMIEGALETIKKSNQPSLSLRYGMEATTLLLCIGNNSGGIRSRKGNYAEASFCYAPLTQKTYFISSPKYAEGIGSIYTGVVTENNSIVVAGEASATKMSRQKIKKIEIYRYEGHGNRFWESLCTAQFRELYALGTIHCDLYILGGQMKLKNQYQITNIVEKYSMEQENWRTLAPLPVPLACHAVVTVKNRLYVIGGWTPQMDLPDDEPDRLSNRTLQYNPSQDKWTECAPMKYSKYRFSTAVVNHEIYVLGGIGCLDRDRGQARQCLDVVEIYNTDRNYWREGPPMPYPLLSLRTNSTCAGAVEGKLYVCGGFHGTARHEVITKEILVLDTWENHWNVASTKALMHDSYDVCLVAQLNTRDLIPPPSDLMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBU7</accession>
    <name>A0A6I9XBU7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RING finger protein 224</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RNF224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913054.1">
        <property type="nucleotide sequence ID" value="XM_014057579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBU7"/>
    <dbReference type="GeneID" id="106541985"/>
    <dbReference type="KEGG" id="tsr:106541985"/>
    <dbReference type="CTD" id="643596"/>
    <dbReference type="OrthoDB" id="252722at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053122">
        <property type="entry name" value="RING_finger_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47454">
        <property type="entry name" value="RING FINGER PROTEIN 224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47454:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="38"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913054.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="18488" checksum="11D29AEC2D0BC23B" modified="2020-10-07" version="1">MQQEQETTALPSGPEDDEAAKPIPEASVPGPGSLKLECVICYCGYDLSARLPRRLYCGHAFCQACIRRLNVTANEQWWIRCPQCRQNTPTPRGGVAMLDLDLAAFLAVKSEKALPRQGGRLQLEQFSKEKPAVTEQPSGSCQDVIPEPQPSCCCPCDPSLCCATTAAFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCA0</accession>
    <name>A0A6I9XCA0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 19">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="19">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">GALNT14</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Golgi apparatus membrane</location>
            <topology evidence="2 19">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013908793.1">
        <property type="nucleotide sequence ID" value="XM_014053318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCA0"/>
    <dbReference type="GeneID" id="106538730"/>
    <dbReference type="KEGG" id="tsr:106538730"/>
    <dbReference type="CTD" id="79623"/>
    <dbReference type="OrthoDB" id="429263at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000063">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000020">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF8">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 19" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 19" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026749216" description="Polypeptide N-acetylgalactosaminyltransferase" evidence="20">
        <location>
            <begin position="34"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="21">
        <location>
            <begin position="420"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013908793.1"/>
        </source>
    </evidence>
    <sequence length="552" mass="64142" checksum="59E43DD9D79C8F23" modified="2020-10-07" version="1">MRRMSRRLALSVLGILWIAALLLFLGARRKLEAAGPEGRQTPKDSDIDWDDLWDQFEERKYLSVRRWKGGEDPYRLYAFNQRESERMPSDRAIHDTRHHRCTTLHYRADLPPTSIIITFHNEARSTLLRTIRSVLNRTPVHLVHEILLVDDFSDDPDDCRLLIKLPKVKCLRNSRREGLIRSRIRGADVAQAGILTFLDSHCEVNKDWLLPLLQRIKEDPTHVVSPVIDIINLDTFAYVAASSDLRGGFDWSLHFKWEQLSSEQKAKRTDPTEPIKTPIIAGGLFVIDRAWFNRLGKYDAAMDIWGGENFEISFRVWMCGGSLEIIPCSRVGHVFRKKHPYIFPEGNANTYIKNTKRTAEVWMDEFKQYYYAARPAAQGRPYGDIQSRVELRKTLKCHTFKWYLENVYPELRIPEESLYQTGMIRQRQRCLESQKNEGQEFPMVILNPCITSKGAAAAAQEWIYTYNQQIHFQQLCLSIHTLFPGSQVMLLPCKEEDGKQRWNKVGSHIEHMATRYCLDTEMVGDSSESMREVVINPCESTAMSQRWEMVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD57</accession>
    <name>A0A6I9XD57_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prostaglandin E2 receptor EP4 subtype</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Prostanoid EP4 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PTGER4</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with FEM1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911777.1">
        <property type="nucleotide sequence ID" value="XM_014056302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD57"/>
    <dbReference type="GeneID" id="106540984"/>
    <dbReference type="KEGG" id="tsr:106540984"/>
    <dbReference type="CTD" id="5734"/>
    <dbReference type="OrthoDB" id="5959154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004957">
        <property type="term" value="F:prostaglandin E receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071380">
        <property type="term" value="P:cellular response to prostaglandin E stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15142">
        <property type="entry name" value="7tmA_PGE2_EP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000101">
        <property type="entry name" value="Prostaglandin E2 receptor EP4 subtype"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001758">
        <property type="entry name" value="Prost_EP4_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF6">
        <property type="entry name" value="PROSTAGLANDIN E2 RECEPTOR EP4 SUBTYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00586">
        <property type="entry name" value="PRSTNOIDEP4R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 16 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="81"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025493"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031869"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013911777.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="56278" checksum="0D443212A6AB468E" modified="2020-10-07" version="1">MLAAFLFPFLSELNFSTLDPPSLSTSPPRFLAFSSRVFFAMALNSTMLPAANGSSNGTGGLPENSKPLTIPMVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTCLVSPVTIATYLKQEWPGGQPLCEYSSFILLFFGLSGLSIICAMSIERYLAINHAYFYSHYVDKKLAALTLFAIYVSNVLFCAMPNMGLGKTKLQYPYTWCFIDWQTNISSHAAFSYMYAGFSSFLIMVTVVSNILVCMALIRMHRQFMRRTSLGTDHATNRVSDFRRRRSFRRMAGAEIQMVILLIATSLVVVICSIPLVVRVFVNQLYRPAIITEIQQNPDLQAIRIASVNPILDPWVYILLRKTVLSKAIEKVKCLFCRIGGARRQHAGNNCHRNSSVTASHSPSFISRELREISSTSQTLLYPLEPSERTVGDRMLLPGTSSCLAQSDTTSIRTLRSSKTSESSQGQECENAFLVTELKPSDRSTPKGSSLQVTFPSETLDLSEKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDK8</accession>
    <name>A0A6I9XDK8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sterile alpha motif domain-containing protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SAMD7</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912291.1">
        <property type="nucleotide sequence ID" value="XM_014056816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDK8"/>
    <dbReference type="GeneID" id="106541388"/>
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    <dbReference type="OrthoDB" id="9943471at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="F:histone binding"/>
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    <dbReference type="GO" id="GO:0045892">
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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    </dbReference>
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        <property type="entry name" value="PcG_chromatin_remod_factors"/>
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    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF89">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 7"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM/Pointed domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="395"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="497"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="296"/>
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            <end position="314"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912291.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="55318" checksum="7F625AB1C84103BC" modified="2020-10-07" version="1">MNPQDHMRKIIILGKQRSVEDGAFYQIGSGIPTGDLRQQHETLMRSHFMTGHLPLMRQHGMQTIPSQLDSRFVERHLLPASEILAPADLRQIHVASALGPPASQLANYPNLLSSCVSQGPGYSFLQSESMEAVAKRQELIQKQIIARMEVSAMFQQKELEKAQRKSLVKSRGFSPYPDVPPCSAGFQEKHYIPEGHLSNGLYIHQTALNELQRNAMFLGTSSYPPLAMLQRERVRRPSRRAGIRKGSRYQFDHGKNPTEDGEANLASGTLGEENEDKKETKGEMPLKPAEAEHEVQAEPSAAVIKNEKEREGGLRKNLCNRKMPREATDGHNGNEKEPHTSCLVFNEKHAEPSKITFSALTSGFSTLNQPPLSFAPPRLILKGEEISSAEDIRKWTVEDVYSFINSLPGCSDYAQVFKDHVIDGETLPLLTEEHLLDMMGLKLGPALKIQSQVSQCLRNIFSTMNIPLAMPNPYATSTSSEPFHDTVSPLPSNSAHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDZ1</accession>
    <name>A0A6I9XDZ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MAPK14</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013909343.1">
        <property type="nucleotide sequence ID" value="XM_014053868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDZ1"/>
    <dbReference type="GeneID" id="106539166"/>
    <dbReference type="KEGG" id="tsr:106539166"/>
    <dbReference type="CTD" id="1432"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07877">
        <property type="entry name" value="STKc_p38alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000769">
        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038784">
        <property type="entry name" value="MAPK14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008352">
        <property type="entry name" value="MAPK_HOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01773">
        <property type="entry name" value="P38MAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0346">Stress response</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR638784-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909343.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="41624" checksum="17AB4F9164A294B4" modified="2020-10-07" version="1">MTQERPKFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCSAFDAKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFSPAKSFEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHINQLQQIMRLTGTPPAYLINRMPSHEARNYIQSLSYMPKMNFENVFIGANPLAVDLLEKMLVLDTDKRITAAEALAHGYFSQYHDPDDEPVADPYDQSFESRELDIEEWKSLTYDEVVSFVPPPLDQEEMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XET2</accession>
    <name>A0A6I9XET2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106542846</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013914164.1">
        <property type="nucleotide sequence ID" value="XM_014058689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XET2"/>
    <dbReference type="GeneID" id="106542846"/>
    <dbReference type="KEGG" id="tsr:106542846"/>
    <dbReference type="OrthoDB" id="9046649at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF12">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="10">
        <location>
            <begin position="199"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="11">
        <location>
            <begin position="710"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914164.1"/>
        </source>
    </evidence>
    <sequence length="923" mass="100569" checksum="E250D9D82578A959" modified="2020-10-07" version="1">MDCKFLGLDDALLEQFIYGLLDLNLHRRILSRTELSMKIAVDEDRAYKMAGQSTPDLHRFPPSVMALFLLRPSSARCPTKSLSPTSMPPSTGCALFPSRTPGPVSAAVAATSVPPAVSEPRSAANAVAITGINSLQLASDIDLLVSEFADVFSEALGCYKGTPISLNLDPLVVPIRLKAHRVPLALRAKVDAELDKLISQGILEPMDHAPWETPIVLPLKSDGSLRVCADYKATVNKALQAHPYPVPVVQHVLHSLGQGKIFAKLDLAQAYQQLPVDDATATTTTIITHRGAFRCTCLQFGISVAPSLFQGLMERLLYGIPGVTPYFNDVLIAAPDQSGLISTLRAVLSHFCAAGLHLKRSKCCLGSHSSTVNFLSFHVDGQGIHPSPAKIEAITNVPTPSSKAELQAFLGLLNFYATFIPHKASYGERLPLLLACDVSPYGMGTVLSHSFPDGSEAPITYYSRTLSRAERNYSHLDKEALVSIAGVKRFHNYLYGRPFTLLTNHKPLFGILAGDRATPRILSPRMSRWSEFLSAYAYRLQYQPGKHMGHMDALSRCLLPLTLSDPAPSIASGVFLINNLALPLSASDITTSTASDLLLSRVLDAVRRGWTADSQAPKFLLFLGRPTELSVLQGCLLWGSRVVMPPALRQLVLRRLHEGHPGIVCMKALGRGYVWWPSMDADITGWVQRCPSCQAVHPVAPSSPPQEWETPSSPWSRLQADFFGPIDGRSFLLVVDALSKWVEVIHLCSVTSAATIHALECLFSTHGLPDTLVTDNGPQFSSTELRLYLASLGIRHAPVAPYHPASNGMAECAVRSAKESLARLSPLPWPTCLCTYLRAQHSTPCAATGKSPAKVLMGRRLHITLDRLHPHFSPLSISLESTPRAFRVGEEVYAQNYLSEPKWVLAVVQELTGPCSYRVQLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFN9</accession>
    <name>A0A6I9XFN9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serum albumin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106539591</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909873.1">
        <property type="nucleotide sequence ID" value="XM_014054398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFN9"/>
    <dbReference type="GeneID" id="106539591"/>
    <dbReference type="KEGG" id="tsr:106539591"/>
    <dbReference type="OrthoDB" id="9875082at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000264">
        <property type="entry name" value="ALB/AFP/VDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020858">
        <property type="entry name" value="Serum_albumin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014760">
        <property type="entry name" value="Serum_albumin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11385:SF14">
        <property type="entry name" value="AFAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11385">
        <property type="entry name" value="SERUM ALBUMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00273">
        <property type="entry name" value="Serum_albumin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00802">
        <property type="entry name" value="SERUMALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00103">
        <property type="entry name" value="ALBUMIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48552">
        <property type="entry name" value="Serum albumin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51438">
        <property type="entry name" value="ALBUMIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026921994" evidence="5">
        <location>
            <begin position="19"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Albumin" evidence="6">
        <location>
            <begin position="22"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Albumin" evidence="6">
        <location>
            <begin position="216"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Albumin" evidence="6">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909873.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="49365" checksum="5FFCCAECF87128FA" modified="2020-10-07" version="1">MKWVIFVSLLCFLSLAELKNLPRRYRNTDDHHWTIRLASQISANDFGAIVLVLFAQALPNTTLEELKTLSANVKAIHQKCVDNQYSSPECTKPLGIVFLDLLCHDEEFSKKYGINDCCAKSDPERHACVLSHKISAIGSVSPFVRPTAEEACQAYHNDRNDVLAHHLYEISRRNARAPVTVLSAATKSFDKILETCCKEVEKDTCIEGKATVVRNKAREIIDEQENTCFNLENYGKETLYELKFIQIAEKFINANLEILTHIAKEVVHIHEATCKGDSLGTLLDRADLSEYVCKHKNDISHNLDHCCEKGLVERSQCIVTKENDPLSPDLPTPSGETFKETACQYYVEHGSINKESFLLALIRTHPELSQLFDLEILHRYGDTLEKCCKREDKVQCLNDGEEQNRLYVKKISDIVKNNCNNYKEIGEYFFQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIG8</accession>
    <name>A0A6I9XIG8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription initiation factor TFIID subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">TBP-associated factor 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TAF8</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TAF8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910713.1">
        <property type="nucleotide sequence ID" value="XM_014055238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIG8"/>
    <dbReference type="GeneID" id="106540195"/>
    <dbReference type="KEGG" id="tsr:106540195"/>
    <dbReference type="CTD" id="129685"/>
    <dbReference type="OrthoDB" id="2193813at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22918">
        <property type="entry name" value="HFD_TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08049">
        <property type="entry name" value="TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000031">
        <property type="entry name" value="transcription initiation factor TFIID subunit 8 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037818">
        <property type="entry name" value="TAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromodomain associated" evidence="13">
        <location>
            <begin position="28"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="234"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013910713.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="34113" checksum="84ADA2A28644EA5C" modified="2020-10-07" version="1">MADPGTGGSNTFSTRSGSKHSTTPADNYYLARRRTLQVVVSSLLTEAGFESAEKAAVETMTEMLQSYICEIGRSAKSFCEHTARTQPTLSDIVVTLVEMGFNVEMLPAYAKRSQRMVITAPPVTNQPVTPKALTAGQNKPHPSHIPSHFPEFPDPHTYIKTPTYREPVLDYQILREKAASQRRDVERALTRFMAKTGETQSLFKDDVSTFPLIAARPFAIPYLTALLPSELEMQQMEETDSSEQDDQTDTENLALQMSMDDSGAEKENASVLQHNTSLSGSRNGEENLIDNPYLRPVKKPKIRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJR4</accession>
    <name>A0A6I9XJR4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21 25">Cystathionine beta-synthase</fullName>
            <ecNumber evidence="7 25">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC106542859</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 25">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 25">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="7 25"/>
    <dbReference type="RefSeq" id="XP_013914177.1">
        <property type="nucleotide sequence ID" value="XM_014058702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJR4"/>
    <dbReference type="GeneID" id="106542859"/>
    <dbReference type="KEGG" id="tsr:106542859"/>
    <dbReference type="OrthoDB" id="728at2759"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00201"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04608">
        <property type="entry name" value="CBS_pair_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000014">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046353">
        <property type="entry name" value="CBS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005857">
        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01137">
        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 25" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17 24 25" id="KW-0129">CBS domain</keyword>
    <keyword evidence="18 25" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0349">Heme</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="19 25" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 25" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CBS" evidence="26">
        <location>
            <begin position="427"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00026192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013914177.1"/>
        </source>
    </evidence>
    <sequence length="562" mass="62066" checksum="7C5BE88002DC0B5F" modified="2020-10-07" version="1">MLDCIPVVGTMPSIPAETEMDTVTCTPVSVDCIPNGILEKESCNSTEKERKWIRPDAVSKCTWELGKSPSESPHHHATLTKTPKIMPDILKNIGDTPMVRINKIGKLCGLKCELLAKCEFFNAGGSVKDRISLRMVEDAERDGILKPGDTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGAEIVRTPTTARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLSHYDTTAEEILQQCEGQVDMIVAGAGTGGTITGIARKIKEKCPGCTIVSVDPEGSILAEPEKLNVTDKTTYEVEGIGYDFIPTVLDRSVVDKWYKCNDEESFAFSRMLIREEGLLCGGSSGSAMSVAVKAAQELKEGQRCVVILPDSVRNYMSKFLSDKWMIQRGFMKEEDDDIKKPWWWHVKVQELCLSAPLTVLPSVTCEKTIEILKEKGFDQVPVVDESGLILGMVTLGNMLSSVLAGKVQPLDKVNKVIYKQFKTIHLQDNLGKLSHILEIDHFALVIHEQIQYHSDGESSKKQMVFGVVTAIDLLNFVTAQEQKRNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKF4</accession>
    <name>A0A6I9XKF4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Gastricsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PGC</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911358.1">
        <property type="nucleotide sequence ID" value="XM_014055883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKF4"/>
    <dbReference type="GeneID" id="106540681"/>
    <dbReference type="KEGG" id="tsr:106540681"/>
    <dbReference type="CTD" id="5225"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000006">
        <property type="entry name" value="Cathepsin E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000004">
        <property type="entry name" value="Pepsin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF70">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07966">
        <property type="entry name" value="A1_Propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026838139" evidence="5">
        <location>
            <begin position="17"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="6">
        <location>
            <begin position="73"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="104"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013911358.1"/>
        </source>
    </evidence>
    <sequence length="388" mass="43531" checksum="31940F50B68B3241" modified="2020-10-07" version="1">MKWLILALVYVHLSEGFHRIILKKGKSIRENMREKGVLEEFLEKYHIDPGLKYQFNKFSATYEPMTNYLDSFYFGKITIGTPPQDFLVLLDTGSGVLWVPSTYCTTKACENHSVFNPRLSSTYYSNNEQTYILRYGFGSLSIMLGYDTVQIQNIIVQNQQFGLSVEEPNYPFYYGNFDGILGLTNPTPNTVGSNTLLHQMMSQNQISEPIFSFYFSRQPTVQYGGELILGGTDPQMYTGEITWAPVTEQAYWQIGIEEFAIGNKATGWCSEGCQGIVDTGTVLLTIPQQYLTDFLQAVNAANDGSYIVDCNNVKNMPTITFFINGSQFPLPPSAYVSNNNGRCSLAIESTYVPSSTGQPLWILGDVFLKEYYSIFDMGNNRVGLAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKS6</accession>
    <name>A0A6I9XKS6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein beta-7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">HSPB7</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914557.1">
        <property type="nucleotide sequence ID" value="XM_014059082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKS6"/>
    <dbReference type="GeneID" id="106543164"/>
    <dbReference type="KEGG" id="tsr:106543164"/>
    <dbReference type="CTD" id="27129"/>
    <dbReference type="OrthoDB" id="9925191at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06479">
        <property type="entry name" value="ACD_HspB7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037885">
        <property type="entry name" value="ACD_HspB7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46907:SF2">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46907">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="34"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914557.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="15687" checksum="16993DA7F39A2828" modified="2020-10-07" version="1">MVHPVCPRIPPWDLLPFASKYVPVLESRIERASLLPTGRSGGMGNIKTFGDTYQFAVDVSDFSPEDIIITTSKNQIEVRAEKLAADGTIVNTFTHKCQLPEDVDPTSLTSTLGADGCLTIKARRGPAKPSEQVQQTFRTEIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKY1</accession>
    <name>A0A6I9XKY1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Protein HEG homolog 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HEG1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914607.1">
        <property type="nucleotide sequence ID" value="XM_014059132.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543202"/>
    <dbReference type="KEGG" id="tsr:106543202"/>
    <dbReference type="CTD" id="57493"/>
    <dbReference type="OrthoDB" id="9946171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24037">
        <property type="entry name" value="HEART DEVELOPMENT PROTEIN WITH EGF-LIKE DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24037:SF3">
        <property type="entry name" value="PROTEIN HEG HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1636"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1416"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="304"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="390"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="522"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="626"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="705"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="783"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="837"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="952"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1076"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1122"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="411"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="705"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="722"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="740"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="786"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="804"/>
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        <location>
            <begin position="837"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="876"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="953"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="972"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1076"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1122"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1152"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1191"/>
            <end position="1201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013914607.1"/>
        </source>
    </evidence>
    <sequence length="1769" mass="191399" checksum="CBE7CD28451B2CA4" modified="2020-10-07" version="1">MSGNSQGPYSTSSVSNSEMYTNGEKVLRTVRESSSQNPRSVSTISSLEMPGTSDPSASSYMIVPADSQQMDLSTQDTDETKATDRHSQSPSATTVTNQMSTESPHTREKCKTPFCSCSQATESYARAMEDTKHMNSRSNTGSKISSSHLDSVTVSRVLVRDEEKSKNVTQTFTTANTSNFSRLSSRSEKTEQMGLLHRDFVVTKEAFTISQAPTEQNSQPLSSSDIKSIASNLYTENRSDRSGNRIIQTLGHTSSSYHPESISTASESEIPSSSALTDSFSVLTPVRTRQNNLSPGFTNVIEAESTHPWDSSQATDAEDSHLRSSSYKENIPTFQRDGVSVSSLSKRNEGSTLRTARDVSSFESDTRTSAVANTTISSNSDFAHSFHMKTPEEIGPDLSPEDTDFTEGMLTPSQAPSKTTVEENGQSQSSSSSTEGIFSTYHLNSFSTSVLSGLDGERTLRTPRDVSNAHDVTQGSIAWHNETSSSSGLTGSSYLLTPVETDLTPQITDFTEGASAHFQASSEKIDVGSSDPPRSSSNIKTRFSHSDQDSASVSGSSDRDDEITLSTSRQVKNVFNITQISSSKNDNITSSFSVADSSYTMTPKDATEMNSSSSGNNDFTEEALTYSEAPSETTDTKSRPHALSNFDIEKKATQTENTFSSTVIQDETFGSLTNQSRSIDTTELSRFDLKNASSSVIIQTLSATLQSRKENPPSTETDFTETLKQSTSSKASSYPSSTKDFSSSTDPFQTIKGSEVGRRFSMASTDSIYLSTTFVHGAERTLRSLPDNSTSDDAANSNPYNPQTSKSSGESLTLSSQLETETYNVSLSKVQSIGPSTEHSLEYSSGVTTALSPNNSATTGSTDNSTSGPRVLPFHTISTGSSAPFSEGGEQTTPPTINTTVMEETESVSSYMETFDSTRSKPSLSKTYSRMTHFSLKDLDVSPSSTEAFQILSSSTDSSSKSELSDAGTEYQPMSGTDFGTETSVSHTDSTYTLTTFAKGGERTLLSLPNNSTSSDFSGSSTFSAEISSASELVQSFFPSDMDFSENSTEPLAVHSLKTPEYSSTVSEPHTTQYYLESESTSTDSLSFSSSSTTRLSEQDSLPPVHPSTLFSSAKSSVPSSFSTFPLSSSPPTSLMTFSPMSPSTKPPTSPFFPSVLPSLSSTPSLLQPSESLETSVSMTEPEGTTGAELHTTRNSHDEDNHLTTVHPLANNTILMATGTSLLTEITEQPANHSLPRTTSQEETKGQTLTLPGISIENESITTTSSPYFQEVTTTSEPAKATTAILLHTIPQKDTFPSSTITMGRKTTEITHTPGRLPTSASTMNYLFTTKLQKVLLPSPTDISSSPGIPTKMVEKSSITTVKTTVPRIVITSPPYTLTSQKTTVQSFPASLIPSRPATTVNLGNTKISPTVYGKDVDECLPNPCPSMSTCTHVQGSFQCICPLGYQMEKGKCNLVRTFVGHFPLMFNTTGGTYAELHQIEGVIINLLNESLSTFPGYYTSTVKASRQEGIVQISILSTFSMASNITFSEVIGTVQSRIQACKAPTLPCQFISNLTQLYRVGGLCKHKDPECDKGTSVCVDLDGIAVCQCKLGYFKYNKLDHSCRACEDGYKLENDTCVSCPFGLGGFNCRNPYQLITIVIAGAGGGLLLIMGIALIVTCCQKNKNDISKLIFKSGDFQMSPYAEYPKNPRVQDWSRETIEMQENGSTKNLLQMTDVYYMPTNLRNPELERNGIYPPYTGLPGSRHSCIYPGQYNPTFISDDTRRRDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XL46</accession>
    <name>A0A6I9XL46_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arylamine N-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106544720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.5" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013916549.1">
        <property type="nucleotide sequence ID" value="XM_014061074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XL46"/>
    <dbReference type="GeneID" id="106544720"/>
    <dbReference type="KEGG" id="tsr:106544720"/>
    <dbReference type="OrthoDB" id="10260017at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004060">
        <property type="term" value="F:arylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2140.20:FF:000001">
        <property type="entry name" value="Arylamine N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF8">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01543">
        <property type="entry name" value="ANATRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013916549.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="33871" checksum="0EBB18838A099BF7" modified="2020-10-07" version="1">MNIQDYFERICYKGSQEKVDLETLTEVFQHHIRAVPFENLSLHCGETIPLDLESAYDKIVKRHRGGWCMENNQLLSWAFQIMGYKVGLLSARVYNPERKAYSEDDNHLLIKVVLEGKTYIVDGGFGMAYQMWEPMELVSGKDQPQTPGIFCFTEKDGVWYFHKVKRKKFRMDSENNIPCPDAIENMICKDIYLFTLQPKMIGDFRMACLQLQTDPSSMFLRKSICSLQTTDGFLALIGWTLIETKYNYKDDTDLVVSTVLTSEEVQKILKERFKIRLDKTFVPLNTCSGPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNJ0</accession>
    <name>A0A6I9XNJ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Anion exchange protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106541393</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912298.1">
        <property type="nucleotide sequence ID" value="XM_014056823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNJ0"/>
    <dbReference type="GeneID" id="106541393"/>
    <dbReference type="KEGG" id="tsr:106541393"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000020">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018241">
        <property type="entry name" value="Anion_exchange_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002178">
        <property type="entry name" value="PTS_EIIA_type-2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF15">
        <property type="entry name" value="ANION EXCHANGE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00219">
        <property type="entry name" value="ANION_EXCHANGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51094">
        <property type="entry name" value="PTS_EIIA_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-2" evidence="11">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013912298.1"/>
        </source>
    </evidence>
    <sequence length="347" mass="39310" checksum="D5EFAE515E1EB90D" modified="2020-10-07" version="1">MHLHSSEGHRGKYIKLMEKIPDDAEATVVLVGCVEFLEQPSMAFVRLNEAVFLESVLEVPIPVRFIFVLLGPSQSNVDYHEIGRSISTLMSDKHFHEAAYMADDRQDLLNAINEFLDCSIVIPPSEVEGKDLLRSIATFQKVLLKKRRERDQKKSLKETNFQESKELCEVKAEEEEEEGEEEEDDPLKRTGIFFGGLVRDIKRRYPKYLSDLRDALHSQCLAAVLFIYFAALSPAITFGGLLGEKTDGLMGVSELIISTSVLGMLFSLLSAQPLLIIGFSGPLLVFEEAFYKFCQSQNIEYLTGRVWIGFWLIVFIIIIVAAEGSFLVRYITPFTQEIFAFLISLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ35</accession>
    <name>A0A6I9XQ35_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106541801</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912813.1">
        <property type="nucleotide sequence ID" value="XM_014057338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ35"/>
    <dbReference type="GeneID" id="106541801"/>
    <dbReference type="KEGG" id="tsr:106541801"/>
    <dbReference type="OrthoDB" id="10069759at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37397:SF1">
        <property type="entry name" value="LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37397">
        <property type="entry name" value="SI:CH211-183D21.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026826135" evidence="1">
        <location>
            <begin position="23"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912813.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="57144" checksum="E728283C10A6ECED" modified="2020-10-07" version="1">MGSGLIYASVLLLCFWLREGNSQNLINDQKMKTTLLINEVNADNPGDDTMEYLELYHVSGQRVALNGYHLVLYNGKGNVAYKVLDLGNLFTDDQGFLLVGSGNLVPRPAIILPKGSIQNGPDAIALYFGRSNLYVGMPVNNENLVDALVHKSNKRDKADELVRVLTPGTEPFLEDPSFRITDDESMERCQGMDSQRFFQLGAPTPGSDNHCIPFSQLNASVGLINEVKLASSLGDAEFVELQGHPSVEVKDLVLVLVEGSTQKIYSAMEVKGETSPDGLLLLGSGLSKIPEGAAHFQNFTFHQNSSTTSLLKAGTNAVALYGGSISHFVVGSRITSTPALLDALVYTTVEQLSSELLDILTPGKPPFYVSKQSQAAGASVSRCLCCSVSGDSSSYALTKPTPLQFNDCPKMRFSQKVSFCFQVTDCQLKPQDESTIQRVLAQSLEKRCNCSFSPAYFKDPVLTCEGREMTFTAVMSARSTDQLDRELQSLSMMEKSEERFRFGNQNNSMAMKTCSSSAEGTGTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRU6</accession>
    <name>A0A6I9XRU6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="6">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106544657</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013916476.1">
        <property type="nucleotide sequence ID" value="XM_014061001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRU6"/>
    <dbReference type="GeneID" id="106544657"/>
    <dbReference type="KEGG" id="tsr:106544657"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000069">
        <property type="entry name" value="Zgc:174895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050120">
        <property type="entry name" value="Adenine_PRTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776:SF7">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="57"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916476.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="24012" checksum="838DA0C5FFDCCE87" modified="2020-10-07" version="1">MDLKTVPASREQGWYLKLMAPNTKGPLYAWLDPSRLYCHPQAFQDCVQDLVRPFRDDAIDQVVGIDAMGFILGAAIAVTLRKGFVTIRKAGHLCVETRTQTYRDYTDREKVMEARTDAIGPGVRVLLVDQWVETGGTMQGAIQLLEQQGSVVAGIAAICMEDSEGGRWLKSHYKWSHCVAPHLMPQFNAHQLDSFQAFRTSLPSQEQPPEHRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS37</accession>
    <name>A0A6I9XS37_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">EP300</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013907555.1">
        <property type="nucleotide sequence ID" value="XM_014052080.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106537821"/>
    <dbReference type="KEGG" id="tsr:106537821"/>
    <dbReference type="CTD" id="2033"/>
    <dbReference type="OrthoDB" id="899at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05495">
        <property type="entry name" value="Bromo_cbp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15646">
        <property type="entry name" value="PHD_p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15802">
        <property type="entry name" value="RING_CBP-p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000001">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1020.10:FF:000002">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.40:FF:000001">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000001">
        <property type="entry name" value="Histone acetyltransferase p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000034">
        <property type="entry name" value="Histone acetyltransferase p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031162">
        <property type="entry name" value="CBP_P300_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003101">
        <property type="entry name" value="KIX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056484">
        <property type="entry name" value="PHD_P300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010303">
        <property type="entry name" value="RING_CBP-p300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038547">
        <property type="entry name" value="RING_CBP-p300_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035898">
        <property type="entry name" value="TAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000197">
        <property type="entry name" value="Znf_TAZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808">
        <property type="entry name" value="CBP/P300-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808:SF29">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE P300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02172">
        <property type="entry name" value="KIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23570">
        <property type="entry name" value="PHD_P300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06001">
        <property type="entry name" value="RING_CBP-p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02135">
        <property type="entry name" value="zf-TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00551">
        <property type="entry name" value="ZnF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57933">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51727">
        <property type="entry name" value="CBP_P300_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50952">
        <property type="entry name" value="KIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50134">
        <property type="entry name" value="ZF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAZ-type" evidence="16">
        <location>
            <begin position="337"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="KIX" evidence="17">
        <location>
            <begin position="569"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="15">
        <location>
            <begin position="1109"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="domain" description="CBP/p300-type HAT" evidence="18">
        <location>
            <begin position="1329"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TAZ-type" evidence="13">
        <location>
            <begin position="337"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="487"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="742"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1256"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1562"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="757"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="785"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="841"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="860"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="910"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="939"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="962"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1027"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1074"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1262"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1562"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1590"/>
            <end position="1600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013907555.1"/>
        </source>
    </evidence>
    <sequence length="1714" mass="189014" checksum="4CCF5449EAFF3D61" modified="2020-10-07" version="1">MAENVVESGPPSAKRPKLSSPALSVSASDGTDFGSLFDLEHDLPDELISSTELGLTNGGDINQLQTSLGMAQDAASKHKQLSELLRAGSSPNLNMGVAGPAQNMASQPQQNNPGMGILNSMVKSPMAQAALTSPNLGMGTGGPNQGPSTQSTAGMMQTVNSPVNQPGMGMNAGINAGMNPGMLSAGNGQGMMQGQVMNGSIGAGRGRSNMSYPNPGMGNAGTLIAETLQQGSPQMGGQASLRGPQPGAMNKMGMMNNPGPYVPPYSQNTGQPIGASGLGPQMQNKPVLPNSLPQFPMDKKPVPAPVMPNMCQQQQTPSVQQASMVSTAMGSGAPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQAGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQSLLGGAAVGIGNSGSVGVGQQTTPNLNTTNQIDPSSIERAYAALGLTYQGNQMPTQTQAQVKSQQQGQSPQGLRPMNPTSANPMGVNGGVGVQAQNLLPDAMLHSAVNSQNPMLNENANVAGLGNMPTATHPSGIGIRKPWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMANLVSYARKVEGDMYESANSRAEYYHLLAEKIYKIQKELEEKRRTRLQRQNMIPNAPVMPPAPMNQGPMGQPQPGMTANGPLPDPNMIRSNVPNQMVNRMQTPPGMNQFGPITMSMPSVGPRQTPPLPHPGQLNQAGNINQIGFPPRMQPQGVGQSSGQSQFVPQNQFPSLSPGMNPASTPMTQPANQTPASQAQMTNSSCPVTSPAVAPGSQGGHIHCPPIPQSALHRNSPSPVPSRTPTPHHTPPGLVSQQQQQQQQQQQQQQQQQQQQPPAPQQATIASAPAAVSQMPPSGPQSQTMHPSQRQTPTPPQAQLTPQMQPPVSVTPSAEQQLPQQPLSQQSTTASVPTPTATLQPQHPTTPLSQPGANIDGQVSNPPSTSSADVNIQQTAPEQQQQQQQQSLPEIKMEVKMEEEDSEHTDHQIDEKPEIKVEPLADECKSEPMQQEEKKPEMKQEVKEEEERPSTPGTQSSPASGQSKRKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLAEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTTINKDQFSKRKNDTLDPEQFVECIECGRKMHQICVLHNEIIWPSGFVCDGCLKKSGRTRRENKFSARRLPTTRLGTFLENRVNEFLRRQNHPESGEVIVRVVHTSEKTVEVKPGMKARFVDSGEMAEQFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPQPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKSVTERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVSKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPQANSLPPIIEPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAMWSTMCMLVELHTQSQDRFVHNCLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSL7</accession>
    <name>A0A6I9XSL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial-processing peptidase subunit beta</fullName>
            <ecNumber evidence="6">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PMPCB</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2. Required for PINK1 turnover by coupling PINK1 mitochondrial import and cleavage, which results in subsequent PINK1 proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of PMPCA (alpha) and PMPCB (beta) subunits, forming the mitochondrial processing protease (MPP) in which PMPCA is involved in substrate recognition and binding and PMPCB is the catalytic subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013917072.1">
        <property type="nucleotide sequence ID" value="XM_014061597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSL7"/>
    <dbReference type="GeneID" id="106545138"/>
    <dbReference type="KEGG" id="tsr:106545138"/>
    <dbReference type="CTD" id="9512"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF103">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="17">
        <location>
            <begin position="41"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="18">
        <location>
            <begin position="155"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013917072.1"/>
        </source>
    </evidence>
    <sequence length="320" mass="35184" checksum="7D829D44196E5D5F" modified="2020-10-07" version="1">MAANTALLHLGKNRFRATNVAPQIVVNVPETKVSTLDNGLRVASEDSGLTTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKASSQTSVEILADIIQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSINRNDLVEYITTHYKGPRIVLASAGGVAHGELLELAKYHFGNLPSIKKEGAPVLPPCQFTGSEIRVRDDKMPLAHLAIAVEAAGWCHPDTIPLMVANTLIGNWDRSFGGGMNLSSKLAQAACHGNLCHSFQSFNTCYTDTGLWGRPLEQLPDYERLCSGMYWLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUL2</accession>
    <name>A0A6I9XUL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Endophilin-A2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Endophilin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SH3GL1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Implicated in endocytosis. May recruit other proteins to membranes with high curvature.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919779.1">
        <property type="nucleotide sequence ID" value="XM_014064304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUL2"/>
    <dbReference type="GeneID" id="106547221"/>
    <dbReference type="KEGG" id="tsr:106547221"/>
    <dbReference type="CTD" id="6455"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000053">
        <property type="entry name" value="endophilin-A1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF63">
        <property type="entry name" value="ENDOPHILIN-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="20">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="238"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="173"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013919779.1"/>
        </source>
    </evidence>
    <sequence length="300" mass="34261" checksum="BF63C11E5C12943D" modified="2020-10-07" version="1">MLNTVSKIRGQVKNPGYPQSEGLLGESMMRYGKELGDDSNFGDALLDAGESMKRLAEVKDSLDIEVKQNFIDPLQNLCDKDLKEIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFEESKEVAETSMHNLLETDIEQVSQLSALVDAQLDYHKQAVQILDELAEKLKRRVRDTSSRPKREYKPKPRETYDFGETVDQSNGGFSCNQAPKVSASSSFRSEKPSRTPSRIFSPHLDQPCCKALYDFEPENDGELGFKEGDIITLTNQIDENWYEGMIHGQSGFFPLNYVEVLVPLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUN8</accession>
    <name>A0A6I9XUN8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SERTA domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SERTAD2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917777.1">
        <property type="nucleotide sequence ID" value="XM_014062302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUN8"/>
    <dbReference type="GeneID" id="106545666"/>
    <dbReference type="KEGG" id="tsr:106545666"/>
    <dbReference type="CTD" id="9792"/>
    <dbReference type="OrthoDB" id="8910518at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052262">
        <property type="entry name" value="E2F-SERTA_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009263">
        <property type="entry name" value="SERTA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16277">
        <property type="entry name" value="CELL DIVISION CYCLE ASSOCIATED PROTEIN 4/SERTA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16277:SF10">
        <property type="entry name" value="SERTA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06031">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51053">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERTA" evidence="2">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013917777.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="34685" checksum="B3EA95831FBE25EB" modified="2020-10-07" version="1">MLGKGGKRKFDEHEDGLEGKVVSPTDGPSKVSYTLQRQTIFNISLMKLYNHRPLTEPSLQKTVLINNMLRRIQEELKQEGSLRPMFLTPSQPTDPLGDNFREAQPAFSHLASPPVHPTDLASPTPLESCLTPASLLEDDTFCTSQTISLDGPPKLPPPTIQPVKDSFSSALDEIEELCPTPTSAEAVVTTAAADTTAPDNSKENSSESSIQKPEGIQENRMSEPKLMDSLPGNFEITTSTGFLTDLTLDDILFADIDTSMYDFDPCTSSTGAASKMAPVSADDLLKTLAPYSTQPVTPNQPFKMDLTELDHIMEVLVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW50</accession>
    <name>A0A6I9XW50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">YPEL5</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917986.1">
        <property type="nucleotide sequence ID" value="XM_014062511.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917987.1">
        <property type="nucleotide sequence ID" value="XM_014062512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW50"/>
    <dbReference type="SMR" id="A0A6I9XW50"/>
    <dbReference type="GeneID" id="106545839"/>
    <dbReference type="KEGG" id="tsr:106545839"/>
    <dbReference type="CTD" id="51646"/>
    <dbReference type="OrthoDB" id="6407410at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013917986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917987.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="13842" checksum="ADCF8C4C3B154EAB" modified="2020-10-07" version="1">MGRIFLDHIGGTRLFSCANCDTILTNRSELISTRFTGATGRAFLFNKVVNLQYSEVQDRVMLTGRHMVRDVSCKNCNSKLGWIYEFATEDSQRYKEGRVILERALVRESEGFEEHVPSDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXC7</accession>
    <name>A0A6I9XXC7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADP-dependent malic enzyme, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106546472</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918822.1">
        <property type="nucleotide sequence ID" value="XM_014063347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXC7"/>
    <dbReference type="GeneID" id="106546472"/>
    <dbReference type="KEGG" id="tsr:106546472"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000060">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF20">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="7">
        <location>
            <begin position="54"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918822.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="36462" checksum="73B51BA67DA07182" modified="2020-10-07" version="1">MNCLIQFEDFANANAFRLLNKYRNRYCTFNDDIQGFSGSPTKEIITNTGNDFSLASAPRTFTKAALGIAHLIVMALEKEGIPREEAVKKIWMVDSKGLIVKGRSHLNHEKEMFAHDHPTVKTLEKVVQTIKPTAIIGVAAVAGAFTGQILKDMGTFNEKPIVFALSNPTSKAECTAEDCYRLTDGRAIFASGSPFPKVTLSDGRTFYPGQGNNAYVFPGVALGIIACGVRHITEEIFLTIAEAIAQEVTEENLAEGRLYPPLSSIRDVSFKIAVKVVDYAYKHNLASWYPEPEDKEAFVKNLIYSPDYDSFIIDNYQWPQEAMKTQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXM3</accession>
    <name>A0A6I9XXM3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMEM5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915358.1">
        <property type="nucleotide sequence ID" value="XM_014059883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXM3"/>
    <dbReference type="GeneID" id="106543805"/>
    <dbReference type="KEGG" id="tsr:106543805"/>
    <dbReference type="CTD" id="10329"/>
    <dbReference type="OrthoDB" id="8560686at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120053">
        <property type="term" value="F:ribitol beta-1,4-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21099">
        <property type="entry name" value="RXYLT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055286">
        <property type="entry name" value="RXYLT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057538">
        <property type="entry name" value="RXYLT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057539">
        <property type="entry name" value="RXYLT1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15576">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15576:SF1">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24785">
        <property type="entry name" value="RXYLT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24786">
        <property type="entry name" value="RXYLT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="RXYLT1 N-terminal" evidence="2">
        <location>
            <begin position="93"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="RXYLT1 C-terminal" evidence="1">
        <location>
            <begin position="242"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915358.1"/>
        </source>
    </evidence>
    <sequence length="437" mass="50553" checksum="FEA57AEE6BE10434" modified="2020-10-07" version="1">MRLTRKRLCFVLVVVYLVFSLYAAYSVFLKPRRPAASRAGQREKQGQEHVVLGVEQWNPWEVNEKIYLQQKSASSFQMLKKISLQQNPTDFRVQIWGKAAIGLYLWEHIFEGLLEPADIKAQWREGSTKIGKSYFSFITGPSVVPGYFSVEADTVVLILNGREEGKIAYATQWLQYAQTLMQTHKLQHVAVVLLGNEQCKNEWIFPYLKSYGGFVELFFLIYDSSIVNEEDIFQWPLGVATYRHFPVVEPSWSMLQGPRPYQCNFLGTVYKNSSREIVLEILMQSGLHKLCWISVREQWIPQETNESLQIYQNALLESDLTLCPVGVNTECYRIYEACSFGSVPVVEDVMTPGNCGNSSVHHRAPLQLLKTMGAPFIFIKDWKELPAVLEKEQNMTLQQKIQRRKILMEWYQQFKAQMRQKFVSTLEKAFLPKDKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY24</accession>
    <name>A0A6I9XY24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="10">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PPIP5K2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013919082.1">
        <property type="nucleotide sequence ID" value="XM_014063607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY24"/>
    <dbReference type="GeneID" id="106546677"/>
    <dbReference type="KEGG" id="tsr:106546677"/>
    <dbReference type="CTD" id="23262"/>
    <dbReference type="OrthoDB" id="18042at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000003">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000001">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000013">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF10">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="12">
        <location>
            <begin position="53"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="844"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="861"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="906"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="952"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013919082.1"/>
        </source>
    </evidence>
    <sequence length="1183" mass="134083" checksum="4233FB6A70BA89CC" modified="2020-10-07" version="1">MSVHATESDIPRFFVGDEDGDEFLDCSRASDFVSFYDQDDDDDEYDSPPERQIMVGICSMAKKSKSKPMNEILERLSMFKYITVVIFEEDVILNEPVENWPLCDCLISFHSKGFPLDKAVAYSKLRNPFVINDLNMQYHIQDRREVYGILKDEGILLPRYAVLNRDPNNPQECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGFFDLFEKCNGYKSGKLKLKKPRQLQEVLDIARQLLIELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNHKQEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQHRVKARLNEILQRDRDFTSEDYEKLTPSGSISLIKSMQVIKNPIKTCDKVYSLIQSLTSQIKQRMEDPKFADIQLYHSETLDLMLRRWAKLEKDFKTKNGKYDISKIPDIYDCIKYDVQHNVSLKLENTMELYRLSKALADIVIPQEYGITEAEKLEIAKGYCNPLLRKIRSDLQRTQDDDTVNKLHPLYSSGVMSPERHVRTRLYFTSESHVHSLLSTLRYGSLCDASKDEQWKRAMDYLNVVSELNYMTQIVIMLYEDPNKEVSSEERFHVELHFSPGAKGCEEDKNLPSGFGYRPASRENDDSKQKSHRNENDEEPHSYKRDETDRSIMMFKPMVSDPIHIHRKSPLSRPRKMGSTEEESPLSVSSPEWLHYTSGVGTGRRRRRSGEQITSSPVSPKSLAFTSSIFGSWQQAPTENNTFFRPPRTIVEQKTSTIGSHCASLFSTSVLGGSSSAPNLQDYARTHRKKLTTSACIDDTTRGSAVKRFSITFARHPTNEHPDLYRCWSVSSEEFLGSSGFELYSMVPSICPLETLHNSLSLKQVDEFLASITASTSCENFPNASTPSFRSPSFIESITERKCSLNTYTPAKIQPTTFEDFAVRRSVERGPSGATSSSVPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ92</accession>
    <name>A0A6I9XZ92_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106539540</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013909815.1">
        <property type="nucleotide sequence ID" value="XM_014054340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ92"/>
    <dbReference type="GeneID" id="106539540"/>
    <dbReference type="KEGG" id="tsr:106539540"/>
    <dbReference type="OrthoDB" id="427186at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="12">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909815.1"/>
        </source>
    </evidence>
    <sequence length="102" mass="11495" checksum="48E8C4CF1ECF45D5" modified="2020-10-07" version="1">MNPEQRAKYLETYDAIRVTHESSAQEGQTVAPSIDEKVDLHFIALVNVDGILYELDGRKPFPINHGESNVDTFLEDAVEVCKKFMERDPDELRFNAIALASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZY4</accession>
    <name>A0A6I9XZY4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rhotekin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106547042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919546.1">
        <property type="nucleotide sequence ID" value="XM_014064071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZY4"/>
    <dbReference type="GeneID" id="106547042"/>
    <dbReference type="KEGG" id="tsr:106547042"/>
    <dbReference type="OrthoDB" id="5817051at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012966">
        <property type="entry name" value="AHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051364">
        <property type="entry name" value="Cytokinesis/Rho-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538">
        <property type="entry name" value="ANILLIN/RHOTEKIN RTKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538:SF21">
        <property type="entry name" value="RHOTEKIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08174">
        <property type="entry name" value="Anillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="177"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919546.1"/>
        </source>
    </evidence>
    <sequence length="388" mass="43166" checksum="8EE5105A92E4CB61" modified="2020-10-07" version="1">MWKNSDHFNSKDRTERYSVFCLFKMGAQVYDSDLLIVDKADTDICLENTTIFEGAGPDFQLKVEVYSCCCGEDPLTLTNTPRKLVKKLKTSLGKSTGKKFSSALDQKDLETLLLTETATHGVKYSLLASASLSLENAKDGFNAHSLTLLGNEDSPSWLPLYGTICCRLVAQPDCMTHDAMTGFLNEQPSGGVPGESWRRRYCVLRGGLLLCFSAPEEREADAEPALSVPINRETHIRPVDKGEKANLFSIINPESGEATARIFQAENRQDLCKWMEAFSQHFFDFSQWKHCCEEFMRMEIRSPQKPPLFLAKEATSVYQELSIDSPVKPLVATAQREEEEEESRVALFVGLDEVDMSGGSSLKQSESIPQGSCDSQGTSKGRDPPSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0P7</accession>
    <name>A0A6I9Y0P7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC106544698 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106544698</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916523.1">
        <property type="nucleotide sequence ID" value="XM_014061048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0P7"/>
    <dbReference type="GeneID" id="106544698"/>
    <dbReference type="KEGG" id="tsr:106544698"/>
    <dbReference type="OrthoDB" id="9899013at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039090">
        <property type="entry name" value="CD7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15343">
        <property type="entry name" value="CD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15343:SF0">
        <property type="entry name" value="T-CELL ANTIGEN CD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026716946" evidence="2">
        <location>
            <begin position="19"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="15"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013916523.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23687" checksum="1E1C6164E0EA3453" modified="2020-10-07" version="1">MLWILSVYFLLLVSPGNAGSKDEMVSQVLIAEEGQSVNLTCKFMHRIEGLFLKRTFGNVKVLYVTQHGKIKTEDPAYENRTEYFEMNNMATITLKHLKENDSDVYLCTAGMFINNELVQKNSSPILLAVEAAAKKNFESSWMLYSIISVLILFIFMLGFYIIYRTNNKKGFQKGHARTKQNFIYEDMNYSLRRKKSETTINPYAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0R8</accession>
    <name>A0A6I9Y0R8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Myelin P2 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106547723</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920437.1">
        <property type="nucleotide sequence ID" value="XM_014064962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0R8"/>
    <dbReference type="GeneID" id="106547723"/>
    <dbReference type="KEGG" id="tsr:106547723"/>
    <dbReference type="OrthoDB" id="412780at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="3">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013920437.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="15430" checksum="617D91918763ABAF" modified="2020-10-07" version="1">MVEQFLGTWKLISSENFDDYMKELGISFAQRQLGSLAKPKIIITMDKDVITIRTETIFTVNEISFQLDQEFEERTIDNRLAKSIVTKDNDTLIHTQKWNGNHTTLKRKIVDGNMVLEYILNDVICTRVYKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y163</accession>
    <name>A0A6I9Y163_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lymphocyte activation gene 3 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LAG3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920131.1">
        <property type="nucleotide sequence ID" value="XM_014064656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y163"/>
    <dbReference type="GeneID" id="106547470"/>
    <dbReference type="KEGG" id="tsr:106547470"/>
    <dbReference type="CTD" id="3902"/>
    <dbReference type="OrthoDB" id="9937043at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042289">
        <property type="term" value="F:MHC class II protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990782">
        <property type="term" value="F:protein tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035723">
        <property type="term" value="P:interleukin-15-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042110">
        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422:SF12">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027106511" evidence="2">
        <location>
            <begin position="20"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013920131.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="19888" checksum="6374940C306A31B7" modified="2020-10-07" version="1">MTLLLLFCTLVLILQMTSGEKYKVWGEEGIGVTLPCYLSPQVVKDNLRHLHKGLEIHWVRHGGSSSMKRHLVLKVKPSGLKSLAHSMMRRVTVWDTGFLKGNFSLQINPLLKEDAGTYEAHVSHGNKDWHCLVELGLVSVTAHPSRPLIESESVRLNCKSSHPGNPTNASSYGSNPAYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y174</accession>
    <name>A0A6I9Y174_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA endonuclease RBBP8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106544856</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916733.1">
        <property type="nucleotide sequence ID" value="XM_014061258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y174"/>
    <dbReference type="GeneID" id="106544856"/>
    <dbReference type="KEGG" id="tsr:106544856"/>
    <dbReference type="OrthoDB" id="5801062at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019518">
        <property type="entry name" value="CtIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033316">
        <property type="entry name" value="RBBP8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107:SF3">
        <property type="entry name" value="RBBP8 N-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107">
        <property type="entry name" value="RETINOBLASTOMA BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10482">
        <property type="entry name" value="CtIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA endonuclease Ctp1 N-terminal" evidence="3">
        <location>
            <begin position="94"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013916733.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="22075" checksum="17F8216E4B237042" modified="2020-10-07" version="1">MSRKAAPVGSTTTTSAPPPAEGQRIQPMLQQEKEKGANFETIMQAIHKIQAGLDSRDDKLDTLVKKSEIVPVTAIFKQQEQVHNVYLLTYIFSNGHSSDNQRIEELFTKNHQLREQQKGLKENVKVLENRLRAGLCDRCQVTQEVAKKKQHEFMKAHFQGLQHIFILTNELNKLREENKSLKEDLKRLYGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y197</accession>
    <name>A0A6I9Y197_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Testis, prostate and placenta-expressed protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TEPP</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920176.1">
        <property type="nucleotide sequence ID" value="XM_014064701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y197"/>
    <dbReference type="GeneID" id="106547496"/>
    <dbReference type="KEGG" id="tsr:106547496"/>
    <dbReference type="CTD" id="374739"/>
    <dbReference type="OrthoDB" id="9970246at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034584">
        <property type="entry name" value="SPMIP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35348">
        <property type="entry name" value="TESTIS, PROSTATE AND PLACENTA-EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35348:SF1">
        <property type="entry name" value="TESTIS, PROSTATE AND PLACENTA-EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22574">
        <property type="entry name" value="SPMIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013920176.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="23439" checksum="915C9A4D3000DD36" modified="2020-10-07" version="1">MAQALDLVPWPPDTSPLYAAPAVLIPLEPKKTMLAGVKDDLYHPCLPTLRRMDMDSMANRLTDQHSRTSTMCCKEDFQRATFTLAGVPSSPLPSKGMLELRRSLGGRYRAGKLAPLPPSTNQEEWPSYTRAMQDWSRFVSAAGELALPSLGGKVLGFSCYAVRYLKPDVTQTWRYCLNQNPSLDRYGPKPLPYNTVNTYRSFGTAHRSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1L0</accession>
    <name>A0A6I9Y1L0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vacuolar protein sorting-associated protein 8 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">VPS8</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910675.1">
        <property type="nucleotide sequence ID" value="XM_014055200.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540164"/>
    <dbReference type="KEGG" id="tsr:106540164"/>
    <dbReference type="CTD" id="23355"/>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16687">
        <property type="entry name" value="RING-H2_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000162">
        <property type="entry name" value="vacuolar protein sorting-associated protein 8 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056939">
        <property type="entry name" value="Znf_RING_Vps8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23412">
        <property type="entry name" value="zf_RING_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="194"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="1252"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013910675.1"/>
        </source>
    </evidence>
    <sequence length="1405" mass="158774" checksum="84D2ED7B66399BED" modified="2020-10-07" version="1">MEEAFKTGTQSLDPDQGCFSMMDESSQQEAPSPKLSITELSKELESKSELIDDKEFDIPQVDTPPTLESILNETDDEEEPFVLEDPSLLNIDNIDTHSYDTSSVASSDSGDKSHLKRKKKLRDSFSIHGSVIRLSLLKGISTQIVSAAAKVDAGLPTAIAASSLIAVGTSHGLALIFGKDPNQALRLCLGNTAVGAQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSIIDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELSFKRVMGVRTCESRCLFSGSKGEVCCIEPLHAKAELRDHPITQYSLLAMASLTKILVIGLKPSLKVWMTFPYGRMEPSSVPLLAWYFVAAQTSVNPVLAFCRGDVVHFLLVKRDESGAIHVTKQKQLHLHYDLINFTWINSHTIVLLDSVEKLHVIDRHTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISNCGGQIFYLGTKSVHVLTLRTWRERIDHLLKQECLNEALALSWSFHEGKAKAVVGLSGDEQKRKAVVADRMVEILIHCADRTMKKCPEQGKIQVMEQHFQTMVPVIVDYCLLLQRIDILFGQMYDKMSENSVAKGVFLECLEPYILSDKLVGITAQIMKDLLLHFQDKNLMANMEACIVHMDITSLDIQQVVLMCWENHLYDAMIYVYNSGMNDYITPLEKLFKAIAHPLSAGKSLSDEQVIMGNKLLVYISCCLAGRAYPLGNIPEDLVPLIKNQVFEFLIRLHSAEAPTDEEVYPYVRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLAQQLAKPNNTLFVNRMLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEENRLIQMAEKAEFYQICEFMYEREHRYDRIIDCYLHDPLRKEEVFSYIHNILSIPGYSTEEKQSVWQKTMKHIEELTSVSPFKTADLISIHFSDEIEQIIQILQDEHLLFQFLKSLLDPREGINQDLLQLSPSITEQFIDLLCRYDADQVLETLKFLDSYRLEETIQVTQKYQLHEASSYLLEKKDDIPGAFLVMLKWLQSKLSTLTSGDKISAQLPLLKDIEDTLGKTIALCQRNSHKFNQQEREALWFPLLEAMLAPQRSALLPRYSEYLKNLTMQVLNNMTTFIALPLILQRILQDPVYGRGKLGEIQGLVLGMLDSFNYEQTLLETTTSLLNRDLHWSLRNLKASMTKGLNPKQDYCCICLQQYKRRQQTGDQIVVFSCGHLYHSLCLLSKECGTETTGQMRWTCFKCSASHKGEKVTLTSLEMRKRTPVSLAQTSSECVLDPQQAQAFDQLCRLYRGTSRLALLTELSRDHGGEKHGFVQVSRGDSALNSVFQNETFQLHLNPPPLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5N7</accession>
    <name>A0A6I9Y5N7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">STAGA complex 65 subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">SPTF-associated factor 65 gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Suppressor of Ty 7-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 16 17 18" type="primary">SUPT7L</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, SUPT7L, GCN5L2, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12 and TAF9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913303.1">
        <property type="nucleotide sequence ID" value="XM_014057828.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913304.1">
        <property type="nucleotide sequence ID" value="XM_014057829.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913305.1">
        <property type="nucleotide sequence ID" value="XM_014057830.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913306.1">
        <property type="nucleotide sequence ID" value="XM_014057831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5N7"/>
    <dbReference type="GeneID" id="106542177"/>
    <dbReference type="KEGG" id="tsr:106542177"/>
    <dbReference type="CTD" id="9913"/>
    <dbReference type="OrthoDB" id="6021257at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06847">
        <property type="entry name" value="HFD_SUPT7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000034">
        <property type="entry name" value="STAGA complex 65 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039460">
        <property type="entry name" value="SUPT7L/Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28598">
        <property type="entry name" value="STAGA COMPLEX 65 SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28598:SF1">
        <property type="entry name" value="STAGA COMPLEX 65 SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Bromodomain associated" evidence="13">
        <location>
            <begin position="149"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="347"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00084075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013913303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013913304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013913305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913306.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="45904" checksum="A92D8A548E6B7AC7" modified="2020-10-07" version="1">MLRYWGEIPVSSSQTNRSSFDLLQREFRTVEVQDPPLHQPSANKSKPTTTLDSPSEPCSLTIHTVQLIQHNRRLRSLIATAQTQNQQQLEGIKMEGSESLPTCPGSPPLPDDLLTLDSKTPNMPFQLRHSDPESDFYRGKGEPVIELSWTSCRQLLYQSIATILAHAGFECAHESVLETLTDIAHEYCLKFAKLLRCAVDREARLGQTPFPDVMEQVFHEVGIGSVLSLQRFWQHRIKDYHSYVLQVSKQLSEEYEKLVNPEKAAEDLKPLKIKEEPVSDITFPMSEELEGDLASGDQSLPVGVLGAQSERFSSNMETEASPQTSGGEVNTSPLWNLAPVKMEPQENEEGNVHGHHGVLGGDVFEEPMSGMSEAGMPQSPNGSESSYGSHSADSLMGSSPVFNQRSKKKMKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5X8</accession>
    <name>A0A6I9Y5X8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106547508</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013920188.1">
        <property type="nucleotide sequence ID" value="XM_014064713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5X8"/>
    <dbReference type="GeneID" id="106547508"/>
    <dbReference type="KEGG" id="tsr:106547508"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05605">
        <property type="entry name" value="STKc_GRK4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF26">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="13">
        <location>
            <begin position="59"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="186"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013920188.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="67708" checksum="5DC3CD327731641E" modified="2020-10-07" version="1">MELENIVANTVLLKAREGGGGKRKGRSKKWREILKFPHISQCDELRKGLERDYNSLCDKQPIGRLLFRQFCETQMELLRCIHFLDAVAEYELSPDEKRKEMGEEVIQQFFNKESLGYMAEICELLMNQCIEDVQRIPCKELFGCCVQRLHEYLAGAPFANYQDSMYFDRFLQWKYLERQSVTKDTFRQYRILGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDNERVIFYAAEICCGLEHLHREGIVYRDLKPENILLDDDGHIRISDLGLAIKIPEGDTIRGRVGTVGYMAPEVINNERYSFSPDWWGLGCLIYEMIEGQSPFRARKERVKREEVEKRVQEDHEKYSDKFSEDAQAICKLLLTKDPKQRLGNKSEGAVQVKQHPFFRTINFKRLEAGILKPSFVPDPRAVYCKDVLDIEQFSTVKGVNLDQTDNDFYVKFATGCVSIPWQNEMIETECFKDLNIFGPNGTRSPDLDWKQLPDPPKRTLLQRLFRRNPSDFTISTNVHSNLSAAPSPAWPMAQAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y622</accession>
    <name>A0A6I9Y622_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19 20">Semaphorin-4G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">SEMA4G</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920308.1">
        <property type="nucleotide sequence ID" value="XM_014064833.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920309.1">
        <property type="nucleotide sequence ID" value="XM_014064834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y622"/>
    <dbReference type="GeneID" id="106547606"/>
    <dbReference type="KEGG" id="tsr:106547606"/>
    <dbReference type="CTD" id="57715"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05872">
        <property type="entry name" value="Ig_Sema4B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001170">
        <property type="entry name" value="Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (Semaphorin) 3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000033">
        <property type="entry name" value="Semaphorin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF17">
        <property type="entry name" value="SEMAPHORIN-4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="679"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="17">
        <location>
            <begin position="34"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="559"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013920308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013920309.1"/>
        </source>
    </evidence>
    <sequence length="844" mass="94986" checksum="76404949E35B2C2F" modified="2020-10-07" version="1">MKEYVLFYLMGLSVATVMGYSSKLSSADLDATPRITIVYNALKYARRFSSSAQNYTTLLLEEEKGILYVGARGAIFSLSSSNISNTTDGSYHKLNWEASPEKQAECLKKGKNNKTECYNHIKLLQRLNSTHLYACGTYAFHPLCAYIDTNKFVLPSHFEEGKEKCPYDPSHGYTGLIVDGSLYTATRYEFRSLPDIRRNRHQRLLRTEESPLHWLNDAEFVASVLVRESTRSSVGDDDKIYYFFTERAGEESTSIFEKTQVPRVARVARVCKNDLGGKKILQRKWTSFMKARLVCYIPYYEVLKDVASLDVGNWTSTVFYATFILSAQWRSIEVSAVCRYNISEVRAAFEGAYMEYQDSSRKWSQYTGDIPEPRPGSCITNRARKRGYNSSQDLPNGVLDFIKLHPLMYDEVKPTDRAPLLIKKNVVYTQVAVDRVQALDGHMYDILFLGTGNGWIHKAVVIGLKVHITEEMQAFKNPQPVENIVISKQQRNLYVGAQSGVLQLPLSTCSQYISCFSCILARDPYCAWDGKSCREVADVLDRSLLIQDVLHGNMGCWNDSVQAVLVQKNRTVLHGGDVLLPCEQTSNLARATWMVNGTKVLAEDGQGHFRLGVDGLLVTDTQLEYSGEYQCYAEENGLQTLVATYALTVLPKELGPQLPSTPTLPSWHLAREDAENMNFIYFVIITVLSGVCFVLIVVLFYVCCLQRRRGKYQLGGPQPSSVELQTVSSNCLSKGRQDEEELSTYPDGCLQIIPGEASTLPLSPKMTPLALPPPPPPLPAEFTNGISTLPNMLRKMNGNSYMLLRQNEEPCTSPLYNSFTEELSKILEKRKHTQLVEKLDESSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6P1</accession>
    <name>A0A6I9Y6P1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="29">Cadherin EGF LAG seven-pass G-type receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC106542847</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor that may have an important role in cell/cell signaling during nervous system formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914165.1">
        <property type="nucleotide sequence ID" value="XM_014058690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6P1"/>
    <dbReference type="GeneID" id="106542847"/>
    <dbReference type="KEGG" id="tsr:106542847"/>
    <dbReference type="OrthoDB" id="26203at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000011">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000059">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000123">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000005">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000183">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000003">
        <property type="entry name" value="Putative cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF347">
        <property type="entry name" value="FI21270P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="18 20" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="851"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="886"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="910"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="953"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="993"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1034"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1062"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="29"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="22">
        <location>
            <begin position="69"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="252"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="24">
        <location>
            <begin position="384"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="26">
        <location>
            <begin position="416"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="25">
        <location>
            <begin position="678"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="27">
        <location>
            <begin position="849"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013914165.1"/>
        </source>
    </evidence>
    <sequence length="1158" mass="131280" checksum="A1EEE38D64E2398D" modified="2020-10-07" version="1">MRNLSIDSKPIDMASYIANNGTLAGCLAQKNYCTTNWCQNGGTCVSKWNTYSCDCPLRYGGKNCEQAMPFPQRFTGESIITWNNLDITISIPWYIGLMFRTRKVNGVLMQADAGASSKIKIQILNKYVVFEVYDGQNQVASLKMTQVRINDGEWHHLLIELKSAKDGKDIKYLAVVSLDYGMYQSTVQIGNQLPGLKMKSIIVGGTSGDLVSVQQGFYGCLQGVRMGETSTNVATLNMKQAVKINVKEGCEIDNPCDSNPCPLHSYCSDDWDSYSCICDPGNYDLYQTEIHAYNLMQENEDTKLAGIANICDGEPMRFLHLSTFQFLCRIDLPCPRGWWGNPICGPCNCEISKGFDPDCNKTNGACRCKINYYRPQKNDSCYPCDCFPSGSHTRTCDMETGQCPCKSGVIGRQCNRCDNPFAEVTPHGCEVVYNGCPKAFEAGIWWPQTKFGQPAAVPCPKGSVGNAVRHCSSEKGWLSPELFNCTTVDFVTLKIMNEKFLHNETKMDSAKSVQIAKVLQNATNHTSSFYGNDVRIAYQMMIRVLQYESQQQGFDLAATRDVEFNENIIKTGSALLDPRTEDLWEHIQRTEGGTAHLLKHYEEYFHNLAKNMKKTYIKPFVIIAANMIIAVDVFEKSNFTGTRVPHFNTIKEDYPKDLESSVLFPETLLKTSIRKVGPTTKPSNYELLLKMEDEIVNNKNTHAKRQRRHPENSSQHAVAMVIIYRTLGCFLPKNYDPDRRSLRLPNRPTINTPVISVIIYRDGNSLPNLVDSPIMIEHVMLETMERTKPVCVFWNHSIATRETAGGWSSKGCELFSRRQNRIICQCNHMTSFAVLMDMSNREYDDVLPLRIITYTTVSISLVALLLTFILLVLIRTLRSNLHSIHKNLVAALFCSELVFLIGINQTENQFVCTVIAILLHYFYMSTFAWIFVEQLHIYRMLTEVRNINFGHMKFYYVMGWGIPAIITGLAVGLDPQGYGNQDFCWLSVYDTLIWSFAGPIVIVVAINTVIFILAVKASCKRRQRSLEKTGVISVLRTAFLLLLLVSATWLLGLMAVNSGIMTFHYLFAIFSCLQGLFIFFFHCIFNKEVRKHLKDTFTGKKSFPDDSTTTRATLLTVRHHFPKAFPCQWIGLEFSFMFVSFWPLKLSIFDNHSVWDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7J6</accession>
    <name>A0A6I9Y7J6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">METTL18</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013915290.1">
        <property type="nucleotide sequence ID" value="XM_014059815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7J6"/>
    <dbReference type="GeneID" id="106543738"/>
    <dbReference type="KEGG" id="tsr:106543738"/>
    <dbReference type="CTD" id="92342"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013915290.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="45931" checksum="1CC0E44B6DC0B91C" modified="2020-10-07" version="1">MYVYICKGIKNSGNYNSVEVNVTLNVGFVHFFLSTNKSSMCLHINAADVNLFIFCIFLKTMEFQFNFSVNKIAENTTDTPENEKLQQKMVLLNNQDNSNITSEENKLNDYLSGTIANISNHSTLNASPDTSLLRKQSCPKSAKVHDIPKDVSKVLENKTIGTLPGIHYANIYVVETALPGNSVKESIMSSQSDLITGVYEGGLKIWECTFDLLDYLSETDIQFAQKSVLDLGCGAGLLGIVALKGNAKEVHFQDYNSSVIEGITIPNVLANYSQNDDGEEDTQLHLNQSRKKDFTQDFLSKCKFFSGEWSEFSTFILNNTKPLVKYDLILTSETIYNPNYYEVFHDALSSLLARKGTIFLASKAHYFGCGGGVHLFTSFIEKKNVFKSQVVKVIEKGLSHFIIELTFKNSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7N8</accession>
    <name>A0A6I9Y7N8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 398-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106543919</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915500.1">
        <property type="nucleotide sequence ID" value="XM_014060025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7N8"/>
    <dbReference type="GeneID" id="106543919"/>
    <dbReference type="KEGG" id="tsr:106543919"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000005">
        <property type="entry name" value="Zinc finger protein 14 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000771">
        <property type="entry name" value="zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF12">
        <property type="entry name" value="RB-ASSOCIATED KRAB ZINC FINGER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="494"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013915500.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="57761" checksum="635CC7D167A53A9B" modified="2020-10-07" version="1">METPWEQNFQWNPAGLPPPPPPGLFWPPPKPGCEAAAHRPTTEISFWTLLAAMQAVERKVDVHTGELRTLRRRADLAEHKLSVTEKMVTDLMPHLDALGTMAQAFGQLHQRVEKLEEQLKNQNFWLLNVPPGTGRETPQVPVNFDNVSVYCSEREWEESNLVPKERQKPAESLVSTDCATSKSGSLSLMEAGTSLSGEHQGPSDDAEDVRKDRAEAAVLLPTSDIAEAKPGNLGQLSLSPGSLEQDVTPSAEEDLVPVDSRMGAIEQSAESGADVANFTTIVVQEGVLPGEGPYICSDCGRSFLYEIQCALHQQSHLQVSADLRDSPTQHHQPEVRAYTCPDCGRWFPHQASLSKHRLWHTGDRPHICAECKKSFRLKINLHLHERTHAVTKKPGCYICGECGRNFNHHSNFLRHQMIHTGERPYTCGECGKTFIRKEHLATHRRLHTGERPYRCALCPKTFTRKQHLVGHQRLHEGEAMWLENHPTQMNEHGQRDGIGVQADDVEPGLGQESYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA51</accession>
    <name>A0A6I9YA51_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-2-glycoprotein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Apolipoprotein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Beta-2-glycoprotein I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106547244</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919815.1">
        <property type="nucleotide sequence ID" value="XM_014064340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA51"/>
    <dbReference type="GeneID" id="106547244"/>
    <dbReference type="KEGG" id="tsr:106547244"/>
    <dbReference type="OrthoDB" id="6103690at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015104">
        <property type="entry name" value="Sushi_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF549">
        <property type="entry name" value="BETA-2-GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09014">
        <property type="entry name" value="Sushi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026649692" description="Beta-2-glycoprotein 1" evidence="14">
        <location>
            <begin position="20"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="82"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="140"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="203"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="23"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="205"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013919815.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="38419" checksum="B2F5BA775276316E" modified="2020-10-07" version="1">MFPVLFSLCLVGITHVALANKVCPRPPEVPLATINVNKEAYSPGEDIIYQCIPGYIPQSSSRRYICHSSGKWPTVTFRCTPKKCPYPGPLNNGEIHVTDLNYLSIIRFSCNTGFVLEGPTSSQCQADGSWNASLPACQPVICPPPPVSEFGALSYHRIESGEDFFFQETIRFSCLLPLALFGSETATCQADGSWTALPECKSVECPPPEPIEHGFINFGLRGIFHYQNTVHYGCYAPYTMDEASESRCEKTGTWSNKPTCKAPCNIPVGKATVLYNGQKITVKKLKHIQHAETIWYFCKSKTEKCSYKTPTQCIDGQLSVPDCFEEYNWIASLFKTDPADMTPCTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA69</accession>
    <name>A0A6I9YA69_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">RNA-binding protein Nova-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NOVA1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with PTBP2; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919905.1">
        <property type="nucleotide sequence ID" value="XM_014064430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA69"/>
    <dbReference type="GeneID" id="106547309"/>
    <dbReference type="KEGG" id="tsr:106547309"/>
    <dbReference type="CTD" id="4857"/>
    <dbReference type="OrthoDB" id="441329at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22435">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22436">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09031">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000019">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000020">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000022">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047275">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047276">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047274">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="48"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="170"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="420"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013919905.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="51737" checksum="BFEF8DBEF5F51AEA" modified="2020-10-07" version="1">MMAAAPIQQNGTHPGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTVEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQTLPSSPTTTKSSPSDPMTTSRANQVKIIVPNSTAGLIIGKGGATVKAIMEQSGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGSPYANTAEVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAH7</accession>
    <name>A0A6I9YAH7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sorting nexin-16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 21" type="primary">SNX16</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homooligomer. Interacts with EGFR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920444.1">
        <property type="nucleotide sequence ID" value="XM_014064969.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920445.1">
        <property type="nucleotide sequence ID" value="XM_014064970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAH7"/>
    <dbReference type="GeneID" id="106547729"/>
    <dbReference type="KEGG" id="tsr:106547729"/>
    <dbReference type="CTD" id="64089"/>
    <dbReference type="OrthoDB" id="76516at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07276">
        <property type="entry name" value="PX_SNX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000011">
        <property type="entry name" value="Putative sorting nexin-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037911">
        <property type="entry name" value="SNX16_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF23">
        <property type="entry name" value="SORTING NEXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="18">
        <location>
            <begin position="103"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="283"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013920444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013920445.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="41967" checksum="645868E151183BE1" modified="2020-10-07" version="1">MTTPYVPVPIPIGSSTNFTINRNQRRSSLGSISTCSDSSKGQIEESSKSNFKKMSIPDEIGTTSCPCNSPLVRTKMLGLNTSMEYYIQPLETTDQNILENWEDRPATPTILGYEVMEERAKFTVYKILVKKNPEENWVVFRRYTDFSRLNDKLKDMFPGFRLALPPKRWFKDNYNPDFLEDRQLGLQAFLQNLVAHKDIANCLAVREFLCLDEPPGPFDSLEESRAFCETLEETNYRLQKELNEKQKEVEALKKLLNEKELYINEMEKRLGDLNLVETKSCKLSGEGSECSGEVESSATEADQRTLEEDTRSEKEDWKTPWNESLVENFVPEIEVAEVAYAADIEILIAQQEKGFATFQCSTSYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAQ4</accession>
    <name>A0A6I9YAQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19 20">Oxysterols receptor LXR-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">NR1H2</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920824.1">
        <property type="nucleotide sequence ID" value="XM_014065349.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920830.1">
        <property type="nucleotide sequence ID" value="XM_014065355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAQ4"/>
    <dbReference type="GeneID" id="106548032"/>
    <dbReference type="KEGG" id="tsr:106548032"/>
    <dbReference type="CTD" id="7376"/>
    <dbReference type="OrthoDB" id="5837785at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07160">
        <property type="entry name" value="NR_DBD_LXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06954">
        <property type="entry name" value="NR_LBD_LXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000014">
        <property type="entry name" value="Oxysterols receptor LXR-alpha isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000017">
        <property type="entry name" value="Oxysterols receptor LXR-alpha isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023257">
        <property type="entry name" value="Liver_X_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF316">
        <property type="entry name" value="OXYSTEROLS RECEPTOR LXR-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02034">
        <property type="entry name" value="LIVERXRECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="9 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12 14 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="16">
        <location>
            <begin position="75"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="17">
        <location>
            <begin position="200"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013920824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013920830.1"/>
        </source>
    </evidence>
    <sequence length="438" mass="49648" checksum="C0A673D459DBCE63" modified="2020-10-07" version="1">MAKTGTDEPQLSQETDCGEPTSQIKNEWSVAKAESQNTVKIEVICTDDGESSTHSTAEEPARKRKKGPAPKMLGDEVCSVCGDKASGFHYNVLSCEGCKGFFRRSLIKGAQYTCKGSGQCHMDMYMRRKCQECRLKKCRQAGMREECVLSEEQIRKKKISKHESDGVPAGERDGGEVLRVPPLLCDPALSQPEPVPLTPQQEGMIQQLVAAQQQCNKRSFSDQPKVTPWPMSSDPNSREARQQRFAHFTELAIISVQEIVDFAKQVPGFLQLSREDQIALLKASTIEIMLLETARRYNHETQCITFLKDFTYSKDDFHRAGLQVEFINPIFEFSRAMRELQLDDAEYALLIAINIFSADRPNVQDHGLVEALQQPYIEALSSYIHLNRPQDHLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBN0</accession>
    <name>A0A6I9YBN0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Hexosyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106548638</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a globoside Gb4Cer (d18:1(4E)) + UDP-alpha-D-galactose = a globoside GalGb4Cer (d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41996"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18259"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:62571"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41997"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Golgi apparatus membrane</location>
            <topology evidence="1 14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013921533.1">
        <property type="nucleotide sequence ID" value="XM_014066058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBN0"/>
    <dbReference type="GeneID" id="106548638"/>
    <dbReference type="KEGG" id="tsr:106548638"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF265">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013921533.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="39092" checksum="17830BEC8F4C06BB" modified="2020-10-07" version="1">MVSPVMKRLQAATKKISFKRQFLIYLLLLVLACLITIILFNCVITFITKESFLELPDINCHKNPPFLVILVSTELGERKTRMVIRKTWGKKRVLADKLIVTFFLLGNHFWQYKPFNVTMESLRYKDIIQKNFIDSYSNLTLKSLMGFEWIHKFCPQATFVMKTDSDMFVNVYYLIELLRKRKSTTQLYTGSIKINEKPIRNPDIKWYISEEEYPENIYPPFCSGTAYVLSSDVASQVYIVSKHITVLKLEDVFIGICVAKLKIQPEKLHSEPIFFPEKVVFSPCHYMKIVTSHFTTPSQNLIYWNALERHMDEKCSNDQKPFQLVYGSYDRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC41</accession>
    <name>A0A6I9YC41_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PDZ and LIM domain protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PDLIM3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play a role in the organization of actin filament arrays within muscle cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921698.1">
        <property type="nucleotide sequence ID" value="XM_014066223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC41"/>
    <dbReference type="GeneID" id="106548775"/>
    <dbReference type="CTD" id="27295"/>
    <dbReference type="OrthoDB" id="1293114at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09450">
        <property type="entry name" value="LIM_ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06753">
        <property type="entry name" value="PDZ_PDLIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000026">
        <property type="entry name" value="PDZ and LIM domain protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000055">
        <property type="entry name" value="PDZ and LIM domain protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF7">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="9"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="293"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="126"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="236"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921698.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="39786" checksum="FD8BAAF21F470816" modified="2020-10-07" version="1">MPQNVLLPGPPPWGFRLSGGIDFNQPLIITRITPGSKASQANLCPGDIIITIDGHSTENMTHNDAQEKIKAATQQLCLKIERAETKLWSPQVAEEGRPHPFKINLEAEPQDIGYFEHKHNIRPKPFVVQSPSRPSGCSTPSGFDGSSGRSTPSSISTVSTICPTELKAAAKMAPNIPLEMELPGVKIVHAQFNTPMQMYSDDNIMETLQGQVATALGETPQISEPAPAVPQSDVYRMLHDDPQGPSQPRQSSSFKVLQDMVSEDSEGRPTGIRSVKAPTTKMGAGAGSVPKVPLCDKCGNGIVGQVVKARDKYRHPECFICSDCNINLKQKGYFFVEGQLYCENHARARTRPPEGGYEAVTVYPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEH2</accession>
    <name>A0A6I9YEH2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor SOX-6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922567.1">
        <property type="nucleotide sequence ID" value="XM_014067092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEH2"/>
    <dbReference type="GeneID" id="106549439"/>
    <dbReference type="KEGG" id="tsr:106549439"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922567.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="13620" checksum="261852D38DC6303D" modified="2020-10-07" version="1">MPSTSEGVVELVGDSKKETRRNSPIVRMSSKQATSPFASDGEGTTIQDLASQEQEDDNNEELVTSHLPLHTILHNKPHSEELPALVTTIQPDSDWNSVISTQHRMIRNIPRNSRTFCSSSGHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEY4</accession>
    <name>A0A6I9YEY4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mixed lineage kinase domain-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MLKL</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922748.1">
        <property type="nucleotide sequence ID" value="XM_014067273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEY4"/>
    <dbReference type="GeneID" id="106549579"/>
    <dbReference type="KEGG" id="tsr:106549579"/>
    <dbReference type="CTD" id="197259"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097527">
        <property type="term" value="P:necroptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013922748.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="19631" checksum="D0FDD54AAA34B072" modified="2020-10-07" version="1">MASDAAAGLYRLHQTGEKCQLHRCINSSKFLVAKGYCVKLSGFKLDQTESSIGRKSKDKVSRPVSSLAYICPEGLASLHHPYNLASEIYSFGIVLWEIATGKLPFTGCNSQEIYEKVHKQKVQEPLSEDCPPYLREIINQCRSYNPSKRPTTEVIVNQLLIDQDDNGSNFMSENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFI7</accession>
    <name>A0A6I9YFI7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Nucleolar protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NOL6</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
            <location evidence="2 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NRAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922937.1">
        <property type="nucleotide sequence ID" value="XM_014067462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFI7"/>
    <dbReference type="GeneID" id="106549742"/>
    <dbReference type="KEGG" id="tsr:106549742"/>
    <dbReference type="CTD" id="65083"/>
    <dbReference type="OrthoDB" id="10251401at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000005">
        <property type="entry name" value="Nucleolar protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000006">
        <property type="entry name" value="Nucleolar protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="177"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="323"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="13">
        <location>
            <begin position="467"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="14">
        <location>
            <begin position="646"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="15">
        <location>
            <begin position="847"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013922937.1"/>
        </source>
    </evidence>
    <sequence length="906" mass="102523" checksum="FDF91588F6948ECD" modified="2020-10-07" version="1">MAARADRRKMLPESDEGTKGPEKEEKQNGIPKEEKPRGKKRASSSEGLLQPVKLGKSELYKVPTNEELSHLKETENLFHSNLLRLQIEELLKEVTLKEKRKQRIDALLHEISGMLNNVPEVPARDITDQSWLPTGVKVPILQIPFKVKGKFHFLPPTQVKVVGSYLLGTCVKPEVNVDVAVIMPREVFQEKDNLNQRYHRKRALYLAHLAQHLAETNRFGSVAFTYMNGNHLKPLVLLQPQGKDAKMVTIRLHACPAPGVFRPLRLHPSKNNVRTAWFTEKDSPGTGVAEPPTPHYNNSILWDLVMETNLLYLSEAASAFRGFQDGVALLKVWLRQRELSQGLGCFNGFMVSMLVGHLLATHRISKMMSAYQVLRNALHFLATTDLTTSGVSLSKDREPGLPSLTEFHQAFQVVFVDSSGLVNLCADMTANTYKQVQFEAKQSMEILDDKTVDGFQLLFMTQKPLLRTFDHVFHLRHIFKLQTACKKMQLLNALMDRGGNYVAAVLPFFLSLLERGLGRRLTLLAHQLPQTQPWSLHVDPPKHKDFGSLSFGLLLNLDFANSVLERGPEADQDEAMEFRQFWGERSELRRFQDGAICEAVLWDAANLCQKRLIPEQIIRHILKLHLDIPDTSISYVGALLEPLIKLRREPAGTGEEDMVSLIRSYDDLSRKLWHLEGMPLTVTAVQGAHPALRYTDTFPPVPMKPDYSFHGKIKGLASFLPVAEKPCPAYVAPIKVICQMEGSGQWPRNKEAIQRIKAAFQMQLAEVLNQQHHLLCRPAITYTDIHKDGYVFRLQVAYHREPQILKELVTPEGMLKYQDTPESQQLELETLHLPFLTSSLHGLQQQYPAFSGSCRLAKRWINAQLLSDSLTEEAVDLLAAFLFLSPAPFTAPSSPQVGFLRFLHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFZ8</accession>
    <name>A0A6I9YFZ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA-directed RNA polymerase II subunit RPB11-a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">POLR2J</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923102.1">
        <property type="nucleotide sequence ID" value="XM_014067627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFZ8"/>
    <dbReference type="GeneID" id="106549871"/>
    <dbReference type="KEGG" id="tsr:106549871"/>
    <dbReference type="CTD" id="5439"/>
    <dbReference type="OrthoDB" id="10248581at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013923102.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14476" checksum="E7646CBFD98ABC2C" modified="2020-10-07" version="1">MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVSQQILTSHNFGGLRAACQRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHY8</accession>
    <name>A0A6I9YHY8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ubiquitin-associated protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106550425</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923806.1">
        <property type="nucleotide sequence ID" value="XM_014068331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHY8"/>
    <dbReference type="GeneID" id="106550425"/>
    <dbReference type="KEGG" id="tsr:106550425"/>
    <dbReference type="OrthoDB" id="5918007at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061484">
        <property type="term" value="P:hematopoietic stem cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14277">
        <property type="entry name" value="UBA_UBP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000004">
        <property type="entry name" value="ubiquitin-associated protein 2-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051833">
        <property type="entry name" value="TC-DDR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022166">
        <property type="entry name" value="UBAP2/Lig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16308">
        <property type="entry name" value="UBIQUITIN ASSOCIATED PROTEIN 2-LIKE/LINGERER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16308:SF19">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12478">
        <property type="entry name" value="UBAP2-Lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="10">
        <location>
            <begin position="53"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="101"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="299"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="465"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="639"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="299"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="422"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="639"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="660"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923806.1"/>
        </source>
    </evidence>
    <sequence length="738" mass="77547" checksum="705DCF7A56415232" modified="2020-10-07" version="1">MMTSVGSERTRGTREKTQIPAAQPTQPQKPVVQATAEQIRLAQMIYDKNDADFEDKVKQLMEVTGKNQDDCMVALHDCNGDVNRAINLLLEGSSDTTLWETVGGKKKSLGKDGSENKENREKRGDREVSRGRGPSSRRGRGGSRGREFRVEENGVETPPGDRPSDRGKRGKGRGFGRARGRGTGRFSSQGMGTFNPADYTDSSATDGFGAKQESWELGENDAAEGTGAWKNSIEEWTAEDWNEDLSETKVFTASSVPAENHVVSGQSIDLVTLLQNPGAPNQDPEAASFDATQQQGFGQALVFTNSQHNPPTASGTSNPSTVNTYSAQSMSSVLSSGFRELSSSKLSNSTGSQILEPLKSPGLGQLASQGPLSTASPVSATPTSSWDVKSSTTQPSILSHFDFKSQPEPSPVLSQLTQRQQQPPPPPAAAVPPPGLEYFASQKAREPLPVDSCAAGNKTVPLSSLCPENQGVPAHQTQQKQIKPSKRRTPPVSKIPSSAVEMPGSTDVLGLNVQFGALEFGLEPAVSEFGPASSYESSPAPNNILYTKPVNEPLNTSLPMSNTAQESTYVSPVVTSSLTCSVQSTSLVSASSSSSSSSYDQASVHNRVTYQSPVPPSEPASVAVMNGHNGVRMQPALDTASSVPTSKLETSPPSLPAQGPSLPSTTASTSLLLPSAPSHTAALPSSDLANSSLSQLSSSLSDHPSSLSSASSHTHNSVENAAALQPSNTFSVAATLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YII7</accession>
    <name>A0A6I9YII7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Protein inhibitor of activated STAT protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">PIAS4</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013924027.1">
        <property type="nucleotide sequence ID" value="XM_014068552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YII7"/>
    <dbReference type="GeneID" id="106550616"/>
    <dbReference type="KEGG" id="tsr:106550616"/>
    <dbReference type="CTD" id="51588"/>
    <dbReference type="OrthoDB" id="10263264at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16821">
        <property type="entry name" value="SP-RING_PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000003">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000009">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.780:FF:000002">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF9">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="24" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="20">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="PINIT" evidence="22">
        <location>
            <begin position="134"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="21">
        <location>
            <begin position="312"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="431"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="463"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013924027.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="55339" checksum="EAACE655DCC1C08A" modified="2020-10-07" version="1">MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKEIYETRYAKISEPGQTPQQQQPQQPLPPPTQHRPPSETLPIHSSYDRNSAVNRTGLSTTPNIDYPSLYGKYLNGLGRLPVKPVKPEVRLVKLPFYTILDELLKPTELVPQNNEKLQESPCVFALTPRQVELIRNSRICYTDTSCAQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITIIWGNYGKSYSVGLFLVRQTTSSELLQRLKNIGIKHPELCKALVKEKLRLDPESEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPATYDQLMIDGLLSKILKECEDADEIEYLVDGSWRPIKAEKERSYSPQCPILVVGGSTGGTSDVNGAPPPAPSVENGKIPADVVDLTLDSSSSEEEEEEEEEDDNGGPKRKVPRLYKNGLASAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIK2</accession>
    <name>A0A6I9YIK2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">LETM1 domain-containing protein LETM2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LETM2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923769.1">
        <property type="nucleotide sequence ID" value="XM_014068294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIK2"/>
    <dbReference type="GeneID" id="106550393"/>
    <dbReference type="KEGG" id="tsr:106550393"/>
    <dbReference type="CTD" id="137994"/>
    <dbReference type="OrthoDB" id="624114at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045742">
        <property type="entry name" value="LETM2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF7">
        <property type="entry name" value="LETM1 DOMAIN-CONTAINING PROTEIN LETM2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19324">
        <property type="entry name" value="LETM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="215"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="395"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="428"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923769.1"/>
        </source>
    </evidence>
    <sequence length="473" mass="53548" checksum="E8689890A3C3D88C" modified="2020-10-07" version="1">MAINSCNIFLAVARSRGSCLLVHSCCSLFSSSAALAQLLDSHHNGTFPKDSESHQLLSSSPQGSAWPHLLIGPMRMLRTSASWHQDKPPPEPSTVKTDPTPAGLPDKAAGTARRSLWQRIVDELRHYYNGFHLLWIDTKVAVRMVWRLLHGQILTRRERRRLMRTSADLFRLVPFLVFIIVPFMEFLLPVFLKLFPEMLPSTFETQSKKEEKQRKKLSAKLELAKFLQETITELARRNKASTGAATQLFSSYVQKVRHSGQQPSTQEIVKFSKLFEDELTLEHLERSQLVILCKLLELQPIGTNNLLRFQLLMKLRSIKADDEMIAKEGVNGLSVSELQSACRARGMRSLGLTEDQLKEQLNQWLDLNLKEKVPPSLLLLSRALYLTDLSPKPMMVNNTKPLKSEDAEMDDDDEVEEQETLLDSAPKVQERKNEEFILPPKDKLPVPEMSVKPPVGETKIEAPRSTKASANGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIQ0</accession>
    <name>A0A6I9YIQ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">L-amino-acid oxidase</fullName>
            <ecNumber evidence="2">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PPOX</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013924248.1">
        <property type="nucleotide sequence ID" value="XM_014068773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIQ0"/>
    <dbReference type="GeneID" id="106550801"/>
    <dbReference type="KEGG" id="tsr:106550801"/>
    <dbReference type="CTD" id="5498"/>
    <dbReference type="OrthoDB" id="419752at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013924248.1"/>
        </source>
    </evidence>
    <sequence length="108" mass="11466" checksum="182ED14D31229154" modified="2020-10-07" version="1">MLGGAWFTSHLGDPDTIPHSELLSRAQAAVKKHLGISAEPLRSIVKVHKSCIPQYSLGHWKHMESATSQLKQHNLPLSLVGASYAGVSVNDCIFSAQTAVAHLAGGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJS1</accession>
    <name>A0A6I9YJS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106551017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:11333"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TENT family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013924492.1">
        <property type="nucleotide sequence ID" value="XM_014069017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJS1"/>
    <dbReference type="GeneID" id="106551017"/>
    <dbReference type="KEGG" id="tsr:106551017"/>
    <dbReference type="OrthoDB" id="10065073at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012937">
        <property type="entry name" value="TET5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12974">
        <property type="entry name" value="PRION-LIKE- Q/N-RICH -DOMAIN-BEARING PROTEIN PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12974:SF30">
        <property type="entry name" value="TERMINAL NUCLEOTIDYLTRANSFERASE 5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07984">
        <property type="entry name" value="NTP_transf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01153">
        <property type="entry name" value="DUF1693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924492.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="44999" checksum="17A5BA7A17431899" modified="2020-10-07" version="1">MTEGLDHRFSYLTWEQIKILDQVLTEVIPIHGRGNFPTLEVKPKDIIHVVKEQLIKKQIVVHDIRLNGSTASHILVKQNGTSYKDLDIIFGVELPSAHEFQVVKESVLNCLLDFLPKGVNKEKITAQTMKEAYVQKMVKVSTDHDRWSLISLSNNSGKNVELKFVNSLRRQFEFSVDSFQIILDTVLDVYSDANGKLTEDAELSVIAESMYGDFEEAMDHLKYKLIATRNPEEIRGGGLLKYSNLLVRDFKPADEVEIKSLERYMCSRFFIDFPDVAEQQRKIESYLRNHFIGEEKSKYDYLMTLRGVVNESTVCLMGHERRQTLNMITILALKVLGEQNIIPNVANVTCYYQPAPYISDRNFSNYYIAHGQPSIIYQPYSFHIHMQSGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJX9</accession>
    <name>A0A6I9YJX9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Poly(A) RNA polymerase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MTPAP</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924466.1">
        <property type="nucleotide sequence ID" value="XM_014068991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJX9"/>
    <dbReference type="GeneID" id="106550998"/>
    <dbReference type="KEGG" id="tsr:106550998"/>
    <dbReference type="CTD" id="55149"/>
    <dbReference type="OrthoDB" id="434989at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041252">
        <property type="entry name" value="RL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF133">
        <property type="entry name" value="POLY(A) RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17797">
        <property type="entry name" value="RL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026702292" evidence="2">
        <location>
            <begin position="26"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="RL" evidence="3">
        <location>
            <begin position="67"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="4">
        <location>
            <begin position="195"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924466.1"/>
        </source>
    </evidence>
    <sequence length="569" mass="63691" checksum="6D7C5148D6249425" modified="2020-10-07" version="1">MAAPTGTLPLLLQWRGLILSRLVVAKRRVGAIRFSSAEAAAVSVGEPQAPGGMAPTKDSENDSRKKTFSEILAERQVEAQRTVLIKCPPNFNTKAFLKYLSQHGNIKNHFFYKSKAMYAVIEFSEKESITSLQDVITIPEFKDEHPIPFKSRLLTLMLKKQSDPISEQTPILCHTQTSLPVEMLVKKLCDAESVSDQLYLLTNECQLTEENTKLRFLACSLIQDIASAYVPDCIVKPFGSSVNTFGKLGCDLDMFLELDKVGKNSAKRKTSSFNLIYQMKKAPSERIASQRILNVLGEFLDNFGPGCEGVQKILNARCPLVRFSHQPSGLQCDLTLNNRIALKSSELLYVYGSLDPRVRALAFSVRYWAWNHGLTSSIPGAWITNFSLTMMVLFFLQRRQPPIVPTLDQLKKLADAEDKEVIEGFDCTFVSNLNKITPTSNTETLDVLLGDFFEFYGNFAFNKNSLNLRKGKEQNKPEASALHIQNPFEENLNISKNVNAAQLQRFVSLARDGAWTLQQVEKRPSDNSKPWGLAALLQASEPGRVGKGFKRLSVLNSDRVKDLLNSLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLZ8</accession>
    <name>A0A6I9YLZ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ankyrin repeat and LEM domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">LEM domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ANKLE2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. May regulate nuclear localization of VRK1 in non-dividing cells. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. Involved in brain development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with BAF/BANF1. Interacts with protein phosphatase 2A (PP2A) components PPP2C (PPP2CA or PPP2CB) and PPP2R1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ANKLE2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925257.1">
        <property type="nucleotide sequence ID" value="XM_014069782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLZ8"/>
    <dbReference type="GeneID" id="106551641"/>
    <dbReference type="KEGG" id="tsr:106551641"/>
    <dbReference type="CTD" id="23141"/>
    <dbReference type="OrthoDB" id="7446186at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12944">
        <property type="entry name" value="LEM_ANKL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000072">
        <property type="entry name" value="Ankyrin repeat and LEM domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.40:FF:000001">
        <property type="entry name" value="LEM domain containing 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056237">
        <property type="entry name" value="ANKLE2_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011015">
        <property type="entry name" value="LEM/LEM-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035006">
        <property type="entry name" value="LEM_ANKL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003887">
        <property type="entry name" value="LEM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF4">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24567">
        <property type="entry name" value="ANKLE2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03020">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00540">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63451">
        <property type="entry name" value="LEM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50954">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LEM" evidence="18">
        <location>
            <begin position="108"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="159"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="621"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="622"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013925257.1"/>
        </source>
    </evidence>
    <sequence length="725" mass="80137" checksum="1769CA4E2B69D86E" modified="2020-10-07" version="1">MERWVAAGWSGWGGLWRAGWPAWELLAACAVIGALGWLLRLLERGPRGGGVGGPRPIATPPEEKNPPPPRCLDRCDGDERPFLPAAAFPLLSPSAASLPGRITMDEIFSRLKQLTPDELREEIVKAGLKCGPITLTTRSIFEKRLAQALWEQQGPSVATAPFSNGIPGTAAADGNQPESGKTNGHLASPEESDFGYSMGLNPPEEESLMREANGSPLHSQNPSKEPLFDLKLTERVHVYEDKKEALQAVKMIKGSRFKAFSNREDAEKFAKGVCDYFPSPNKSTVSLSPVKVCSVFGRDGLCSPELETVSKERANSYKSPRTQDLTAKLRKAVEKGDESAFLELIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQPGVCRLLLDTLENPEFMRLMYPDDDTVMLEKRISYIVDLYLNTPDKMVFDTPLHFACKFGNPDVVSVLASHPGIVKNPKNKYGQTPADVICERNKNKSADLKTKIREHLEGQCYVPLFRAEDNATSPVIGAPWSPDQTESSPLASLPRYLGSPKDPMLTVRAYAGPLSPSKAEDFRRLWKTPPRERAEYFYHLRKSDLERGVERVGRELAHELGHPWGEYWEFLGCFVDLSSQEGLGRLEGHLSQQQEMSSNAQLGENEAHNKPPQAHGKSKNCNSVSVGAFLDDDDMSLEEVKNRQNAARNYSPVIAPNELPTIGTFGSSKCDILAIEQTGTIMPRTKTSIRPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMV9</accession>
    <name>A0A6I9YMV9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-phosphatidylinositol 3-phosphate 5-kinase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106552088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925753.1">
        <property type="nucleotide sequence ID" value="XM_014070278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMV9"/>
    <dbReference type="GeneID" id="106552088"/>
    <dbReference type="KEGG" id="tsr:106552088"/>
    <dbReference type="OrthoDB" id="9369719at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052810">
        <property type="term" value="F:1-phosphatidylinositol-5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032438">
        <property type="term" value="P:melanosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903426">
        <property type="term" value="P:regulation of reactive oxygen species biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043548">
        <property type="entry name" value="PIKfyve"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46715">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46715:SF1">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013925753.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16786" checksum="7B6E3E0A51622BDC" modified="2020-10-07" version="1">MIADNGGQQMISISDAFIKESLFNRRVEEKSKDLFFTPLGWHHSNLDLLREENGEKQAMERLLSANHNHMMALLQQLLYNEALSLSWRDIIVPVVCQVVQTVRPDVKNRDDDMDIRQFVHIKKVSHSVCLELKKKRRKEGGELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNF0</accession>
    <name>A0A6I9YNF0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Dynein heavy chain 10, axonemal-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106551655</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925275.1">
        <property type="nucleotide sequence ID" value="XM_014069800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNF0"/>
    <dbReference type="GeneID" id="106551655"/>
    <dbReference type="KEGG" id="tsr:106551655"/>
    <dbReference type="OrthoDB" id="64868at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000010">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000007">
        <property type="entry name" value="Dynein axonemal heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF63">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="200"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="548"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="800"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925275.1"/>
        </source>
    </evidence>
    <sequence length="859" mass="97882" checksum="176D5CBEA4EA5742" modified="2020-10-07" version="1">MVFVDPKNLRYRPYWDKWTKERSKQEKQLLDRLFDKYVPCLIEMIVEGIVDGRQGEKMKMIIWQTDLNMVTQLSMMLDALIETEVEDPDILECYFLEAIYCSLGASLLEAGRGKFDDFIKRISSMSTVNDEMILAKPGELPGQLPTLYDFHFDGRTKRWVPWSSLVAQYHHSPAVRFIDILVPTVDTTRTTWLLEKMVKIKRPMVLVGESGTSKTATTQNLLRKLNPDTNVLLVINFSSRTTSMDIQRNMEANVEKRTKETYGPPMGKRLLVFMDDMNMPRVDEYGTQQPIALLKLLLEKGALYDRGKEMNCKYLRDLGFIAAMGKAGGGRNEVDPRFISLFSVFNVPFPSEVSLHLIYASILKGHLATFDESISAIADKITTCTLTLYETIVRDLPPTPSKFHYIFNLRDLSRVYNGLVQTTPERFQTVTQMVRVWRNECLRVFHDRLINKADKALVQGHIQHLLEANFKDSAENAIRDPILFGDFRMALNEGEPRVYEDIQDYDAAKALFQEILEEYNEVNTKMNLVLFDDALEHLTRVHRIIRMDRGHALLVGVGGSGKQSLARLAAYTAGCEVFEIVLSRGYSENNLREDLKILYTRLGIENKVIVFLFTDAHVAEEGFLELINNMLTSGIVPALYADDERDNILSQITQEAMKTGIAPAKESVWQYFVNKSANNLHIVLGMSPVGETLRTRCRNFPGLVNNTGIDWFLPWPPQALYAVAKFFLGNNPLIPSEHTEELIAHIVMVHGSVGVYSKKFLQKLRRSNYVTPKNYLDFINTYTKLLEDKNHFILAQCKRLEGGLDKLKEATIQLDELNLKLAEQKIVLAEKSAACEALLQEIATNTAIGKFTIPALPEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNG3</accession>
    <name>A0A6I9YNG3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="32">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="37" type="primary">GRIN2A</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="37">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="32">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24 32">Postsynaptic cell membrane</location>
            <topology evidence="24 32">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="28">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925454.1">
        <property type="nucleotide sequence ID" value="XM_014069979.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551820"/>
    <dbReference type="KEGG" id="tsr:106551820"/>
    <dbReference type="CTD" id="2903"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
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    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0017146">
        <property type="term" value="C:NMDA selective glutamate receptor complex"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0004972">
        <property type="term" value="F:NMDA glutamate receptor activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007611">
        <property type="term" value="P:learning or memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0045471">
        <property type="term" value="P:response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd06378">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13718">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000593">
        <property type="entry name" value="Glutamate ionotropic receptor NMDA type subunit 1"/>
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        <property type="entry name" value="Glutamate ionotropic receptor NMDA type subunit 2B"/>
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        <property type="entry name" value="Glutamate receptor ionotropic, NMDA 2B, putative"/>
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        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
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        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
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    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018884">
        <property type="entry name" value="NMDAR2_C"/>
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    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10565">
        <property type="entry name" value="NMDAR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 32" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="23" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16 31" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="22 32" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14 32" id="KW-0406">Ion transport</keyword>
    <keyword evidence="21 32" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19 32" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="17 32 37" id="KW-0675">Receptor</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 32" id="KW-0732">Signal</keyword>
    <keyword evidence="13 32" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 32" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="32">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027157627" description="Glutamate receptor" evidence="32">
        <location>
            <begin position="24"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="817"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="34">
        <location>
            <begin position="435"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="35">
        <location>
            <begin position="436"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1016"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1148"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1247"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="1016"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="1064"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="1158"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="686"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="728"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="30">
        <location>
            <position position="659"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="30">
        <location>
            <position position="692"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="31">
        <location>
            <begin position="742"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023257"/>
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            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00060784"/>
        </source>
    </evidence>
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            <dbReference type="PIRSR" id="PIRSR601508-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
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            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="RefSeq" id="XP_013925454.1"/>
        </source>
    </evidence>
    <sequence length="1453" mass="162880" checksum="78C5BB6B7DD8A7C6" modified="2020-10-07" version="1">MVLVKMACVCWNLLVLHAFLVHGAERSSPILNVAVILGRTPSVTERDLRALSSRDVAGGLPTDVNVVPLLINQTDPKSIITHICDLISGTKIHGVLFGDDSGQEAVAQILDFISAQTLIPILGIHGGSSMIMAEKDEMSTFFQFGATIQQEAIIMLKFMQYYDWHIFSVVTSIFPGYRDFISFVKYTVENSFVGWDMQNVVTLDAAIGDAKSTIQLKKIHSSVILLYCSTDEAAFILDEARSLGLTGYDFFWIVPSLVSGSMETPPDNFPAGLISVAYDGWDYTLDARIRDGLGIITTAAASMLQKYSFIPEAKTSCYGQSEYNEQPSHMLHKFMMNVTWKGRDLSFTTDGYQAQPRLVVTVLNESRAWEKVGEWENHTLTHKYSVWPRFNSFNDSDPDHNHLSIVTLEEAPFVIVEDMDPLTETCVKNTVPCRKFVVINNITKEGKNLKKCCKGFCIDILKKLSRTIKFTYDLYLVINGKHGKKINNEWNGMIGEVVKKQAVMAVGSLTINGERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLVVSAIAVFIFEYFSPVGYNRNLAQGKDAHGPSFTIGKAVWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQQPYAYSPPFRFGTVPNGSTERNIRNNYKEMHAYMVKYNQKGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRAIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMGLSLITFVWEHLFYWKLRFCFTGMCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPHFTLTSSQTNMLKLLQTAKNMTSVNPGRISSPQRAAEFIRRGSLMMDLMMEKGSFIFSDNNHPLTPKENIFSDNANELQAFPATRHPDNLNNYVFQGPHPLTLHESNPNTVEVAMAAEAKENSRPRQLWRKSLESLRQDSLRQSHGSQRDDGTGGNRRHSLKGPRYLPEDVHSDVSDTSSRAAGHAEEERNSKPPKTKENAKKRPASKYPKDCSEVELTYLKSKQSLPRDKIYIIEADKQHSFHLDVPPEYVENIILPEPADFSNLYQDRNDSYRPPEQSNQIPLSNEESLPSNGQYKLYPKHYGTKDKNVAYGEANERYRQKATHCRSCLTSLPSYAGHYLSRSPYRCGACVRMGSLYDIEEGQMLQSPANSGPREEPCQQDWAPEEKSQPPRKSKLQISRQRSFDNLFNKHRDGNGGRTARSISLKEKERLLEGGPYATLFRAPQSKHSSGKLPLFSRTLDDGKRIKSLYLECSAENPFLRSYCDDQHLVLAPSPTSLHRQPAYDSVSRSSVKSAASYCSRDGRVHNDACVSEHGMPYAATQNSLYSAPRGLNSCSDRRVYKKIPSLESDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP24</accession>
    <name>A0A6I9YP24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Glucosylceramidase</fullName>
            <ecNumber evidence="19">3.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC106551827</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:70255"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:72956"/>
            <dbReference type="ChEBI" id="CHEBI:73432"/>
            <dbReference type="ChEBI" id="CHEBI:189066"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:70256"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:70257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a beta-D-galactosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:14297"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18390"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.46"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:14298"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a beta-D-galactosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + an N-acylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:70235"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:18390"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:189066"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:70236"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:70237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:13269"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.45"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:13270"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:58264"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:58265"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:58266"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a beta-D-xylosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:70239"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:189067"/>
            <dbReference type="ChEBI" id="CHEBI:189068"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70240"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>beta-D-glucosyl-(1&lt;-&gt;1')-N-(15Z-tetracosenoyl)-sphing-4-enine + cholesterol = N-(15Z-tetracosenoyl)-sphing-4-enine + cholesteryl 3-beta-D-glucoside</text>
            <dbReference type="Rhea" id="RHEA:70315"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
            <dbReference type="ChEBI" id="CHEBI:74450"/>
            <dbReference type="ChEBI" id="CHEBI:76302"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:70316"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:70317"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>beta-D-glucosyl-(1&lt;-&gt;1)-N-octadecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + N-octadecanoylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:70311"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
            <dbReference type="ChEBI" id="CHEBI:72961"/>
            <dbReference type="ChEBI" id="CHEBI:84719"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:70312"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:70313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4E-enine + cholesterol = N-(9Z-octadecenoyl)-sphing-4-enine + cholesteryl 3-beta-D-glucoside</text>
            <dbReference type="Rhea" id="RHEA:58324"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
            <dbReference type="ChEBI" id="CHEBI:77996"/>
            <dbReference type="ChEBI" id="CHEBI:139140"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:58325"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:58326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>beta-D-glucosyl-N-dodecanoylsphing-4-enine + cholesterol = N-dodecanoylsphing-4-enine + cholesteryl 3-beta-D-glucoside</text>
            <dbReference type="Rhea" id="RHEA:70307"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
            <dbReference type="ChEBI" id="CHEBI:72956"/>
            <dbReference type="ChEBI" id="CHEBI:76297"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:70308"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:70309"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>beta-D-glucosyl-N-octanoylsphing-4E-enine + cholesterol = N-octanoylsphing-4-enine + cholesteryl 3-beta-D-glucoside</text>
            <dbReference type="Rhea" id="RHEA:70303"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
            <dbReference type="ChEBI" id="CHEBI:45815"/>
            <dbReference type="ChEBI" id="CHEBI:65222"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:70304"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:70305"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>beta-D-xylosyl-(1&lt;-&gt;1')-N-(9Z-octadecenoyl)-sphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + N-(9Z-octadecenoyl)-sphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:70251"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:77996"/>
            <dbReference type="ChEBI" id="CHEBI:189067"/>
            <dbReference type="ChEBI" id="CHEBI:189081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:70252"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>cholesteryl 3-beta-D-glucoside + H2O = cholesterol + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:11956"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:11957"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Steroid metabolism; cholesterol metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyl hydrolase 30 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.45" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013925460.1">
        <property type="nucleotide sequence ID" value="XM_014069985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP24"/>
    <dbReference type="GeneID" id="106551827"/>
    <dbReference type="KEGG" id="tsr:106551827"/>
    <dbReference type="OrthoDB" id="2160638at2759"/>
    <dbReference type="UniPathway" id="UPA00296"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004336">
        <property type="term" value="F:galactosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004348">
        <property type="term" value="F:glucosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006680">
        <property type="term" value="P:glucosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000426">
        <property type="entry name" value="Glucosylceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001139">
        <property type="entry name" value="Glyco_hydro_30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033453">
        <property type="entry name" value="Glyco_hydro_30_TIM-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11069">
        <property type="entry name" value="GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11069:SF23">
        <property type="entry name" value="LYSOSOMAL ACID GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02055">
        <property type="entry name" value="Glyco_hydro_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00843">
        <property type="entry name" value="GLHYDRLASE30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0746">Sphingolipid metabolism</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026966469" description="Glucosylceramidase" evidence="20">
        <location>
            <begin position="23"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 30 TIM-barrel" evidence="21">
        <location>
            <begin position="101"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013925460.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="37313" checksum="D4A874293C4821B0" modified="2020-10-07" version="1">MASVPIHLLVLVFLSAVAKTSTGSRACNRKYFGVDAIVCVCNSTYCDTQDPVRLPPLGQFVMYESSKSGNRLERREGHFQNKTTNPGILLILESAQRYQSIKGFGGSVTDSASINILALSPNAQRNLLRSYFSEEGIEYNLVRMPMASCDFSIRPYTYDDFPHDYELKNFSLVLEDLQMKIPIVHRAKAMSRKPISLMASPWTAPIWMKSNEDWKGEGWLKGQAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGYVKNYPFQTLGFTPESQRDFIALDLGPALARSSHKDIRLIILDDNRIHLPRWAKVVSGRLKTSKTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR25</accession>
    <name>A0A6I9YR25_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Osteocalcin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">BGLAP</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Binds strongly to apatite and calcium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="11 12">Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the osteocalcin/matrix Gla protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926090.1">
        <property type="nucleotide sequence ID" value="XM_014070615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR25"/>
    <dbReference type="GeneID" id="106552364"/>
    <dbReference type="KEGG" id="tsr:106552364"/>
    <dbReference type="CTD" id="632"/>
    <dbReference type="OrthoDB" id="9950568at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046848">
        <property type="term" value="F:hydroxyapatite binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008147">
        <property type="term" value="F:structural constituent of bone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031214">
        <property type="term" value="P:biomineral tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060348">
        <property type="term" value="P:bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030500">
        <property type="term" value="P:regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900076">
        <property type="term" value="P:regulation of cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032571">
        <property type="term" value="P:response to vitamin K"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058704">
        <property type="entry name" value="BGLAP-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039176">
        <property type="entry name" value="Osteocalcin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002384">
        <property type="entry name" value="Osteocalcin/MGP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14235">
        <property type="entry name" value="OSTEOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14235:SF0">
        <property type="entry name" value="OSTEOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25890">
        <property type="entry name" value="BGLAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00002">
        <property type="entry name" value="GLABONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0091">Biomineralization</keyword>
    <keyword evidence="7 9 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 11 12" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="6 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027157551" description="Osteocalcin" evidence="12">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="14">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="4-carboxyglutamate" evidence="11">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-carboxyglutamate" evidence="11">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-carboxyglutamate" evidence="11">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="79"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602384-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602384-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926090.1"/>
        </source>
    </evidence>
    <sequence length="105" mass="11042" checksum="429541B8358D9907" modified="2020-10-07" version="1">MNALLLATLCALAALGLAHADAAQPRSVDGSPKSAAFVSKREGAEMVKRQKRNLNSGYGAAGAAPQVAVNPLEAQREVCELSPACDELADHIGFQEAYRQFYGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRR4</accession>
    <name>A0A6I9YRR4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Chloride channel protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106552534</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926315.1">
        <property type="nucleotide sequence ID" value="XM_014070840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRR4"/>
    <dbReference type="GeneID" id="106552534"/>
    <dbReference type="KEGG" id="tsr:106552534"/>
    <dbReference type="OrthoDB" id="4564at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="10" id="KW-0868">Chloride</keyword>
    <keyword evidence="9" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="182"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013926315.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="25631" checksum="840B6F9A8CCD6902" modified="2020-10-07" version="1">MGESMAAWFPDGIHTDSNIYRIVPGGYAVVGAAALSGAVTHTVSTAVIVFELTGQISHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGHQEKYNVRVEDIMVRDVRYITLNCRYRDLQDVLHSTKMKSLALVESAESMILLGSIDRTQIGALLHDQLSYIRRRQFIQEKTQLEHRVPEKSQENKEPHHAPETGVRFQIVPEESSSFLPPQNEPRKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS71</accession>
    <name>A0A6I9YS71_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Solute carrier family 25 member 33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SLC25A33</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927393.1">
        <property type="nucleotide sequence ID" value="XM_014071918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS71"/>
    <dbReference type="GeneID" id="106553426"/>
    <dbReference type="KEGG" id="tsr:106553426"/>
    <dbReference type="CTD" id="84275"/>
    <dbReference type="OrthoDB" id="269120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000028">
        <property type="entry name" value="Solute carrier family 25 member 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000032">
        <property type="entry name" value="Solute carrier family 25 member 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF5">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="114"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="219"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013927393.1"/>
        </source>
    </evidence>
    <sequence length="309" mass="34512" checksum="252C5E335508E85A" modified="2020-10-07" version="1">MDMEPLLCGGTVGAIFTCPLEVIKTRLQSSRLAFRAVYYPQVHLGTLSGEGVVRPTSLPPGVVQVLKSILEKEGPKSLFRGLGPNLVGVAPSRAVYFACYSRAKEQFNHVFVPNSNMVHISSAASAAFVTNSLMNPIWMVKTRMQLERRVCGSKQMNTLQCARYVYRSEGIRGFYRGLTASYAGISETIICFAIYERLKQLLKDPIRSPFTGNPEKNSTNFIGITVAAAISKGCASCIAYPHEVIRTRLREEGTKYQSFLQTARLVFQEEGYFAFYRGLFAQLMRQIPNTAIVLSTYELIVYLLEEQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSY1</accession>
    <name>A0A6I9YSY1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tumor necrosis factor ligand superfamily member 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927417.1">
        <property type="nucleotide sequence ID" value="XM_014071942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSY1"/>
    <dbReference type="GeneID" id="106553443"/>
    <dbReference type="KEGG" id="tsr:106553443"/>
    <dbReference type="OrthoDB" id="5983780at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927417.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18806" checksum="A291F257BC07BC4D" modified="2020-10-07" version="1">MHLSVMQHNQYCGYPQIFWTNNSSDCAPQTGLYHSSGIVADQDICLPLPVPARKRHRQNHRDGTRLCFLVVFLLILLAITGIGLAMFQIFHLQKQLQKFSSSGCVPPSPDMLKGALNRTEEKKVKRSAHLTGKHQKHILLMHLIIFPCFPNIPPEGFVPFVGLKPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT33</accession>
    <name>A0A6I9YT33_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Protein FAM150B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">FAM150B</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ALKAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927256.1">
        <property type="nucleotide sequence ID" value="XM_014071781.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013927257.1">
        <property type="nucleotide sequence ID" value="XM_014071782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT33"/>
    <dbReference type="GeneID" id="106553309"/>
    <dbReference type="KEGG" id="tsr:106553309"/>
    <dbReference type="CTD" id="285016"/>
    <dbReference type="OrthoDB" id="9807651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030298">
        <property type="term" value="F:receptor signaling protein tyrosine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070378">
        <property type="term" value="P:positive regulation of ERK5 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029364">
        <property type="entry name" value="ALKL1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28676:SF2">
        <property type="entry name" value="ALK AND LTK LIGAND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28676">
        <property type="entry name" value="ALK AND LTK LIGAND 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15129">
        <property type="entry name" value="ALKL1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636660" evidence="10">
        <location>
            <begin position="24"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927257.1"/>
        </source>
    </evidence>
    <sequence length="156" mass="18334" checksum="E03ABBD6B4BAE805" modified="2020-10-07" version="1">MCKLLRPSVLIGLIALILSAGHCKEKPETGVGLKERQNLLNLIMEIIQELKKYHLEEDEENGVQYKQDYPPLDQREMPDYGTYTEEEQKVEIFPRDLRMKDKFLNHLTGHLYFGPKCTKHFHRLYHHTRDCTIPAYYKRCARLLTRLAVSPMCTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTV1</accession>
    <name>A0A6I9YTV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Choline transporter-like protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106553159</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Choline/H+ antiporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>choline(out) + n H(+)(in) = choline(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75463"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927075.1">
        <property type="nucleotide sequence ID" value="XM_014071600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTV1"/>
    <dbReference type="GeneID" id="106553159"/>
    <dbReference type="KEGG" id="tsr:106553159"/>
    <dbReference type="OrthoDB" id="420519at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF42">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013927075.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="20677" checksum="2D77444B5ECC9D61" modified="2020-10-07" version="1">MAKKKEFYYGKNGEPRKFDPKFNGPIHNRHCTDVLCCGIFVVVILGYIALGIVAWVHGDPRKVAYPTDSYGQFCGQKDTPNENKTILFYFNILKCASPIVLINLQCPTTQLCVSKCPDRFATYIEMQSSYRSYWEYYKQFCKPGFTKPRKSVTQVMRDEDCPSMIIPSRPCKYLCIYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTZ3</accession>
    <name>A0A6I9YTZ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Helicase with zinc finger domain 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106553193</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927115.1">
        <property type="nucleotide sequence ID" value="XM_014071640.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106553193"/>
    <dbReference type="KEGG" id="tsr:106553193"/>
    <dbReference type="OrthoDB" id="2285229at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001373">
        <property type="entry name" value="Helicase with zinc finger domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056787">
        <property type="entry name" value="OB_HELZ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF16">
        <property type="entry name" value="HELICASE WITH ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25049">
        <property type="entry name" value="OB_HELZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="7">
        <location>
            <begin position="775"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="481"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013927115.1"/>
        </source>
    </evidence>
    <sequence length="1634" mass="187105" checksum="89130EA955080459" modified="2020-10-07" version="1">LQFCQWHQAVDNLWDVKLVFPDVAACSIPCGTSQVPWGNAKQKQAFSFITGQVTGFCRTPPLLIYGPFGTGKTFTLAKTTLEIIKKSKTRVLICTHTNSAADIYIREYFHTYAISSHPEAIPLRVKSIYRSVKSTDPITLRYCCLSPSGNEFCLPTKEQIDRHRIVLTTCMASQDLAVPCGYFTHILIDEAAQMLECEALVPLSLASQTTMIILAGDHMQKTPRLYSLHKDEQSADYTLLNRLFQHYKKEKHEVATKSRIIFNENYRSTAGIIEFVSKHFYVGREDTIRAKGNVPPHPEFYPLMFCHVSSSAEKDRSMSWYNISEVEQIFEKVQEMDQKWPVEWGQKNLKSICVVSYGIQVKFIRDKLRRKGFSDVTVESYDNLAGREFRVIIINTVHNCNSLAHVSSSNLEYFNNARVLNTIITRAQSQVIVVGDAVALCSHGQCSKIWKSFIKKCIEKKSVIPETLTLEEIKQVMSDMASWNRGNPESEEEDSDTDSWVSDTESLNLDDPIMQELLDESKKLMVLVTEEGLLKVESDVSVPERRRQEYTNYSLKMMQQYLQMQPQRYKRCEFLQERFDQASAFTLDDASSMTIQIKGRVNCGMAFTGDKVLVEILTPNATLDGNPHGKVVGILEKVKQDLTFVCKLDEFDPRVMIPIDQSVTKIFVPPLKDNTDSIPIRRVDQAGKIKLKSWKKITPENRKTWLFVVQIMKWEEGYYYPLGIVTEVLPMTSSLEDGLRILDLEYSLSNGYPASVIRQVTKLISGHPSLMRAERKDCRAYFTFTVDPKGAKDLDDAISVKDMDDKYEIGIHIADMASVIPKDCPLDTEAKNRGLTYYVPEKPIHMFPPELSQSLCSLLPHQERLAISLFVVIDKATDQIERISFAMSIICSDRQMTYEEAEGIIKNYYKIEAPSPNFDTLEDCVAMVYHFSRVHRKFRLHEDCHYDQLDEEIPLGQRCSHQMIEELMILFNSSVGDLLTNRLPTRNLTPLRCQMEPNPQQISRMREKYRDVIPLSTHLSHHLGVPTDGAPVRTIDPFVLLTSSWEHLKSAANNRDFPKMLDLITTDDIHPKLAPINLEFRKLLNRSYFLRSNSCPQAKLGHYSLHVDSYTWATSPIRRYVDVVVQRHIVSLISKKPVQYSQKEIEFLCHDFNRKNGKANTYMRRVRSLELATQLKCQVQKKFAFIANVEGKAKNFKLIFPLNKETFPDSPSINYRALQLVSQPNFIEEQNSMRLKWKRRMYSLSTKNKDEARRSGIGGNIFRFNGDVWHKILAAIKNEDYENLIELLEKDYTLESKPVGSMGRSKCLHYKELSVELKVGDVLNFQLTTNVQRGFLVPFVQLWTVAPGFEVCLEHTERPIDCFSRYASQALKDFYKSASDYRKVWLPLCDIEATLGALAENNSIVLQDVPILWKARRTSQGQLCGTIKLTKEFLKDSAIEVDFGACYMCIRLSGLCGHEIQDKEEDLSQNFQKLSLTKEASKTQDFMIDPNTYTWVAHGCTDKSDQNEKPDQRGEVMVNFYIHFTSMEKIPFEVTQTSSRFTVELIPKQLPDVRKEKAVWDLENATELARSIALGQPIPVPRQKPSNILKRRDFNIPGYSRKLNPSQQSAIQEALKKSFTLIQGPPGEGSASFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU04</accession>
    <name>A0A6I9YU04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Poly [ADP-ribose] polymerase 8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PARP8</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927464.1">
        <property type="nucleotide sequence ID" value="XM_014071989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU04"/>
    <dbReference type="GeneID" id="106553485"/>
    <dbReference type="KEGG" id="tsr:106553485"/>
    <dbReference type="CTD" id="79668"/>
    <dbReference type="OrthoDB" id="109543at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01341">
        <property type="entry name" value="ADP_ribosyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051838">
        <property type="entry name" value="ARTD_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21328">
        <property type="entry name" value="POLY ADP-RIBOSE POLYMERASE FAMILY, MEMBER PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PARP catalytic" evidence="7">
        <location>
            <begin position="621"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013927464.1"/>
        </source>
    </evidence>
    <sequence length="728" mass="82209" checksum="DF46D4856B386C66" modified="2020-10-07" version="1">MGMCSRQERIQKDIDIVIQKCKAEKDCLFADFRYADSTFTFTYIGGSKSISYSVHVSEDYPDNTYVSSSDNDEDVLVTTEPIPVIFHRISTELRKTNDVNCCLFIKSKLQRENGEESRHCTVVEEDSEGDNDSEEFYYGGQVSYDGELHKHPQLEADLAAVREIYGPNAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWDVIRTEPIIVRLHCSLTQYLNGPVPTIDVFQISTKERFGLGHQLKKIMQTFVTQQWKNGKEKSNSTHNKKLSEKKVKSPLHIFSTLRRSPSYPPPGCGKNKSKLKPEQDGISKTHMLLRRTCSSTVKSEDSCVAKSHRTFGRSLSSEHTMTIKSHKLLNHSCSATIKQDEGITLKSHKLLNRPCSGDMRCEHNSTLKPHKLLSRSYSSNLRMDELDGLKNHKLLSKSYSSAPKSSKMELFKEPTITEGRRLSLTSGLIGILTPSTSSPSPSAVRKRFSSPNYGAKSVPIRDHGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDSQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDSLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKTLYGSTFAFHGSHIENWHSILRNGLVVASNTRLQVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUD7</accession>
    <name>A0A6I9YUD7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PFDN2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92. Interacts with URI1; the interaction is phosphorylation-dependent and occurs in a growth-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927651.1">
        <property type="nucleotide sequence ID" value="XM_014072176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUD7"/>
    <dbReference type="GeneID" id="106553637"/>
    <dbReference type="KEGG" id="tsr:106553637"/>
    <dbReference type="CTD" id="5202"/>
    <dbReference type="OrthoDB" id="29646at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000002">
        <property type="entry name" value="Prefoldin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="38"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013927651.1"/>
        </source>
    </evidence>
    <sequence length="163" mass="17578" checksum="7B1B5A2B858A530D" modified="2020-10-07" version="1">MAEESKSKGGPVAATPAGPAGGGGAAKPLTPEQVVAGFNRLRQEQRGLASKAAELEMELNEHKLVIETLRDVDPSRKCFRMVGGVLVERTVKEVLPALENNKEQINKIIETLSQQLQTKGRELNEFREKHNIRLMGEEEQKPPAKESPEGAAAKSSSAGVLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUR6</accession>
    <name>A0A6I9YUR6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553418</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927385.1">
        <property type="nucleotide sequence ID" value="XM_014071910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUR6"/>
    <dbReference type="GeneID" id="106553418"/>
    <dbReference type="KEGG" id="tsr:106553418"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="10">
        <location>
            <begin position="39"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927385.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="27962" checksum="E2415244FC2FCD2B" modified="2020-10-07" version="1">MCVVSSLRINSFTSYWRSLELKKQRFLATFVLLALEEARRVAPLLVEEAAFPLPSAVLVIFRRHHDTPIVKANHRNLAYILLVSLLLCFLCSFLFIGKPRKFTCLFRQTSFAILFSFAVSSLLAKTVMVVLAFMATKPGNRTRKLLGKSLTNSTVLACPLVQAFLCATWLGTSPPFLNLDFHSLFAEIILECNEDSASMFYAALAFLGFLSLISFIVAFLARKLPDSFNEAKLITFSMLSIGAVTNISLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVG0</accession>
    <name>A0A6I9YVG0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Intraflagellar transport protein 20 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">IFT20</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928598.1">
        <property type="nucleotide sequence ID" value="XM_014073123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVG0"/>
    <dbReference type="GeneID" id="106554458"/>
    <dbReference type="KEGG" id="tsr:106554458"/>
    <dbReference type="CTD" id="90410"/>
    <dbReference type="OrthoDB" id="10254896at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030990">
        <property type="term" value="C:intraciliary transport particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028172">
        <property type="entry name" value="FT20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31978">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31978:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14931">
        <property type="entry name" value="IFT20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="102"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928598.1"/>
        </source>
    </evidence>
    <sequence length="155" mass="17442" checksum="B3AA24CB52B75BC4" modified="2020-10-07" version="1">MSFAIISSFQVAGETSRPSSREQERAGLRRVPKSSPGVPSSPQPSAATATEETTALKEECRLFVDKIAEFKKIVGNLIELVDQLAKATENEKIKAIGARNLLKSIAKQREAQQQQLQALIAEKKTQLERYRVEYQTLCKIEADQNEFIDQFIFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWD7</accession>
    <name>A0A6I9YWD7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Unconventional myosin-X-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106554755</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928963.1">
        <property type="nucleotide sequence ID" value="XM_014073488.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554755"/>
    <dbReference type="KEGG" id="tsr:106554755"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060002">
        <property type="term" value="F:plus-end directed microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051489">
        <property type="term" value="P:regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17206">
        <property type="entry name" value="FERM_F1_Myosin-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13297">
        <property type="entry name" value="PH3_MyoX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000008">
        <property type="entry name" value="Putative unconventional myosin-IXa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000195">
        <property type="entry name" value="Unconventional myosin-X"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="Actin_motor_Myosin"/>
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        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="UNCONVENTIONAL MYOSIN-X"/>
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    <dbReference type="Pfam" id="PF00373">
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    <dbReference type="PRINTS" id="PR00193">
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    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="501"/>
        </location>
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            <end position="1093"/>
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            <begin position="1240"/>
            <end position="1440"/>
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            <begin position="1244"/>
            <end position="1290"/>
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    <feature type="region of interest" description="Actin-binding" evidence="9">
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            <end position="403"/>
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    <evidence type="ECO:0000259" key="12">
        <source>
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        <source>
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    </evidence>
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    </evidence>
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            <dbReference type="RefSeq" id="XP_013928963.1"/>
        </source>
    </evidence>
    <sequence length="1440" mass="166695" checksum="46AE0EC913D6BE55" modified="2020-10-07" version="1">MPPKDLLEKNRVVRQNPGERNYHIFYALLAGIEGEKKDAFYLSAPENYHYLNQSGCVADKTINDAEAFKEVIMAMEVMQFTTEEVRDVLRLLAGILHLGNIEFITAGGAQVSFKTALNRSAELLGLDSTQLTEALTQRSMILRGEEILTPLNIQQAIDSRDSAAMALYSQCFAWVIKKINSRIKGKDDFKSIGILDIFGFENFEVNRFEQFNINYANEKLQEYFNKHIFSLEQLEYSREGLQWEDIDWTDNGECLDLIEKKLGLLALINEESHFPQATDKTLLAKLHIQHANNPFYVKPRVAVHNFGVKHYAGEVQYDVRGILEKNRDTFRDDLLNLLRESSHDFIYDLFEHVSSRSGQDTLKCGSKHRKPTVNLQFKDSLHSLMATLSTSNPFFVRCIKPNMQKMPDQFDQNVVLNQLRYSGMLETVRIRRAGFPVRRPFQDFHKRYKVLMRNLTLPEDLKGKCTALLHLYDSTKTEWQLGKSKVFLRESLEHTLEKEREIEVSKAAMIIKAHILGYMARKRYKKVLYYVVIIQKNYRAFYLRRRYLLLKKAAITFQKQMRGLIARRVYRQLLEEKKQQEEEKRQREEEERKQRKMEAERLAQQAKEALAAITKPPKELKQDLESHKENKQVEEILRLEKEIEDLQRMKEQQELTLTEASLQRLQQLRDEELKRLEDEACRAAQEFLESLNFDEIDECVRNIERTLSVNGSFCTELSDVTEAEKPNFNFSQPYPEEEVDEGFEADDDAFKDSPNPSEHGHSDQRTSGIRTSDDSSEEDPYMNDTVVPASPATGNNTLIIPSILDSLSLHNSSSGESTYCMPENVSRQPPNAAELISPEADYDYDQDDYEDGAITSGSSVTFSNSHSSQWSPDYRCSIGTYNSSGAYRFSSEGAQSSFEDSEEDFDSRFDTDDELSYRRDSVYSCVSLPYFHSFLYMKGNLFKKQPNSFVIITANRVLHCNADTPEEMHHWITLLQRSKGDTRVEGQEFIVRGWLHKEVKNGPKMSSLKLKKRWFVLTHNSLDYYKSSEKNALKLGTLVLNSLCSAVPPDEKIFKETGYWNVTVYGRKHCYRLYTKLLNEATRWSSAIQNVIDTKAAIDTPTQQLIQDIKENCLNPEVVEQIYKRNPILRYTQHPLHSPLLPLPYGDINLNSKFWLRSLWDMPVYYGKKPLLNKKLCREKTKCNQKMKFNISPHRVHDQFPATEMAKYALFIYESLKKTKGREFVPSRDEIETLINRQEMTSTVYCHGGGSCKITINSHTTAGEVVEKLIRGLGMEDSRNMFALFEYNGTTDKAIESRTVVADVLAKFEKIGATSDSDHEPWKFYFKLYCFLDIEHVPKDSVEFAFMFEQAHEAVIQGHFPALEETLQVLAALRLQYLQGDYSSSNVTAVEMEDVYPLQRLKSRIVQSTNFFVPTERFERKRSNFLEGTLRRSFRTSSMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXD6</accession>
    <name>A0A6I9YXD6_9SAUR</name>
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        <submittedName>
            <fullName evidence="5">SET-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SETBP1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929318.1">
        <property type="nucleotide sequence ID" value="XM_014073843.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tsr:106555074"/>
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    <dbReference type="OrthoDB" id="9937744at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147:SF2">
        <property type="entry name" value="SET-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="46"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="239"/>
        </location>
    </feature>
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        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
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            <begin position="295"/>
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            <begin position="587"/>
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            <begin position="839"/>
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        </location>
    </feature>
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            <begin position="886"/>
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        </location>
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            <begin position="1121"/>
            <end position="1142"/>
        </location>
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            <begin position="1186"/>
            <end position="1209"/>
        </location>
    </feature>
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        <location>
            <begin position="1226"/>
            <end position="1245"/>
        </location>
    </feature>
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        <location>
            <begin position="1418"/>
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        </location>
    </feature>
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        <location>
            <begin position="1494"/>
            <end position="1547"/>
        </location>
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        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
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            <begin position="199"/>
            <end position="220"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="255"/>
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            <begin position="343"/>
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        <location>
            <begin position="420"/>
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        </location>
    </feature>
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        <location>
            <begin position="442"/>
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        </location>
    </feature>
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        <location>
            <begin position="471"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
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        <location>
            <begin position="839"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="895"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="918"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1129"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1226"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1427"/>
            <end position="1436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929318.1"/>
        </source>
    </evidence>
    <sequence length="1547" mass="170799" checksum="68F3AE2860EBD328" modified="2020-10-07" version="1">MEPRENLGPSRPRGSETDFLASTITSAKAPSALAGSGEILLSASSSAKGISGSGDHHRMEIEEEDELCSRRDVDSISNADSEKWVAGDGLEEQEFSIKEANFTEGSLKLKIQTTKRAKKPPKSLENYICPPEIKITIKQSGEPKLSRGGKNSKASREEDHAHSKKKGYDRLQKHSVVHYDPELPQDFTSDTLKSRHQQRNSNQHHLDWSLTSESGSTSQACFVAPESNREATNSPKISGLEAGLSFSKSQVKKANSSSAWAQLTSGGGNKDHSSCTAAAGSNALGLASQSNNLVDCSGFLNQEGGGQADPLDQTSTSSKKKSSKKDLINQTTLNTDLEWVKNPQKSLEGQPNVNMDGKKEPSATKSPSPRQSPNSANLSENDTSHVRITIPIKGPIGEPPGHKRKKRQSTKVAPDKTEKSTFPGQPVNSDVASRSSTQVDLCQKDPRVTKPAKAPEIESPSVVSMEKNEMTNKSPSSNATRLKKTPTVTSVPVTNDIPAPSKHLEIQHPKFSAKRRWTCSKPKNMFREPSLSPSEKLMLEPPSAYPITPSSPLYTNTDSLTVITPIKKKRGRPKKQPLLTVETIHEGTSTSPVSPISREFPGTKKRKKRRNLVNLTQMPLGEEHSAGELKFHKKVGKLGVLDKKTIKTINKMKTLKRKNLLSQILSCSSNISLKAKTQPPATSGAPTIDARLGKQINVSKRGTIYIGKKRGRKPRSELQSPQEDPKAVIKHLRPVSSQPDHPAVPSTLQSLVTSSPATVHPLSMQLAGSHDNLSPARTDTNFSELKTMPNLQPISALPTKTPKGIHSGTWKLSPPRLMANSPSHLCDIGSLKEITLSPLSESHSEETIPSDSGIGTDNNSTADQAEKTSESRRRYSFDFCSLDNPEAVQSDTSSKNRHSHRPKHLMVDNFLSHESMKKPKHKRKRKNLQNRDDLQFLADLEELISKFQVFRISHRSYTFYHENPYPSIFRINFDPYYPVPYIQYDPLLYLRRTSDMKSKKKRGRPAKTNDTMTKVPFLQGFGYPIPSGSYYAPYGMPYTSMPMMNLGYYSQYPAPLYLSHTLGTASPFMRPTVPPPQFHPSSHMKMPNTAKHKAKHGVHLQTPVGMGLGEVHSSLTSAKVSGTSLSSGRLHKRKHKHKHKHKEERILGAHDDLSSLFASKSPGFSSHSVGERLNVSEKDISLLTEKGKHKDKQKHQHCEAGHKGGAKSSFEVDTLSTLSLSDTQQWVKEKNNSSSDDPCLKKYAGNTESSTRADVMDVFGEMSSASDKRDGDSSGAKRRSFDGFGTYREKDFQTFRASRKDRNSYDSSTVSGMTSSHLKADQALLHSKNECSALAGVTRRKPAALNGIAVSSTPMLSLLPSSTAAETASSTLKKRFKRCEIEAIQCEVRKMCNYTKILSTKKNLDHVNKILKAKRLQRQSKTGNNFVKKRRGRPRKQPALFDEDSGDQMPVLEKCVDLPYKRGQRLLLNSLILEQASGQDPVAATIEAVVHMARETSESPQHPVPPSRTSRGGKRKSKSQPHPEDEEEEVKGKRHRKNRGSENEHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXD7</accession>
    <name>A0A6I9YXD7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Coiled-coil domain-containing protein 90B, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106554753</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCDC90 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928962.1">
        <property type="nucleotide sequence ID" value="XM_014073487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXD7"/>
    <dbReference type="GeneID" id="106554753"/>
    <dbReference type="KEGG" id="tsr:106554753"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000015">
        <property type="entry name" value="Mitochondrial calcium uniporter regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF14">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 90B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013928962.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="26452" checksum="94230DC93D48C0EE" modified="2020-10-07" version="1">ADNSTVPSFSGLAASASAQSYDMRRVEITPLEQRKLTFDTHRLVRDLEAHGFAKEQAETVVSALMNLSAVSLDTVYKDMVTQGQQEITLQQIMFHLDSIRKDMVILEKSEFANLRAENEKMKIELDQVKQQLMNETNQIRAENKLDINLERSRVTDMFTDQERKLMENSTEFHKKDSNTNSSLTEVNNKIDAEIAALKTLMESNKLETIRYLAASVFTCLAIALGFYRFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXJ2</accession>
    <name>A0A6I9YXJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Elongation of very long chain fatty acids protein 6</fullName>
            <ecNumber evidence="19">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">3-keto acyl-CoA synthase ELOVL6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ELOVL fatty acid elongase 6</fullName>
            <shortName evidence="19">ELOVL FA elongase 6</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Very long chain 3-ketoacyl-CoA synthase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Very long chain 3-oxoacyl-CoA synthase 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 22" type="primary">ELOVL6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that elongates fatty acids with 12, 14 and 16 carbons with higher activity toward C16:0 acyl-CoAs. Catalyzes the synthesis of unsaturated C16 long chain fatty acids and, to a lesser extent, C18:0 and those with low desaturation degree. May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z)-hexadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(11Z)-octadecenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:76555"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:39676"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(9Z)-octadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(11Z)-eicosenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36511"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74011"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:36512"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(9Z,12Z)-octadecadienoyl-CoA + malonyl-CoA + H(+) = (11Z,14Z)-3-oxoicosa-11,14-dienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36503"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:74012"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:36504"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(9Z,12Z,15Z)-octadecatrienoyl-CoA + malonyl-CoA + H(+) = (11Z,14Z,17Z)-3-oxoeicosatrienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:74034"/>
            <dbReference type="ChEBI" id="CHEBI:74054"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:36524"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dodecanoyl-CoA + malonyl-CoA + H(+) = 3-oxotetradecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:60140"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:62543"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:60141"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hexadecanoyl-CoA + malonyl-CoA + H(+) = 3-oxooctadecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35315"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:71407"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:35316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tetradecanoyl-CoA + malonyl-CoA + H(+) = 3-oxohexadecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:39168"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="19">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Endoplasmic reticulum membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="19">N-Glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ELO family. ELOVL6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013929378.1">
        <property type="nucleotide sequence ID" value="XM_014073903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXJ2"/>
    <dbReference type="GeneID" id="106555129"/>
    <dbReference type="KEGG" id="tsr:106555129"/>
    <dbReference type="CTD" id="79071"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03206">
        <property type="entry name" value="VLCF_elongase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033675">
        <property type="entry name" value="ELOVL6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF125">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 19 20" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 19 20" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 19 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 19 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 19 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 19 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013929378.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="31279" checksum="AADE25727C3CAF3A" modified="2020-10-07" version="1">MNMSVLTLQEYEFEKQFNENEAIQWMQENWKKSFLFSALYAAFIFGGRHLMNKRAKFELRKPLVLWSLSLAVFSIFGALRTGAYMVYILMTKGLKQSVCDQSFYNGPVSKFWAYAFVLSKAPELGDTIFIILRKQKLIFLHWYHHITVLLYSWYSYKDMVAGGGWFMTMNYGVHAVMYTYYALRAAGFRVSRKFAMFITLSQITQMFVGCIVNYLVFAWMQQGQCHSHVYNVVWCSLMYLSYFVLFCHFFFDAYIGKTRKARKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYM3</accession>
    <name>A0A6I9YYM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Type 2 lactosamine alpha-2,3-sialyltransferase</fullName>
            <ecNumber evidence="19">2.4.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">ST3Gal VI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Sialyltransferase 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ST3GAL6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Transfers the sialyl residue from CMP-N-acetyl-beta-neuraminate to the terminal galactose residue on sugar chains of glycoproteins and glycolipids. It's alpha-2,3-sialyltransferase activity is specific toward type II glycan chains (Galbeta1-4GlcNAc) on glycoproteins and glycolipids such as neolactosides nLc4Cer and nLc6Cer, whose sialyl-products serve as precursors for the Lewis X antigen. Critically involved in the synthesis of functional selectin ligands needed for neutrophil recruitment during inflammation and lymphocyte homing to the lymph nodes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52316"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:136545"/>
            <dbReference type="EC" id="2.4.3.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:52317"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a neolactoside nLc4Cer(d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a neolactoside IV(3)-alpha-NeuAc-nLc4Cer(d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17006"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:58665"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.4.3.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:18914"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a neolactoside nLc6Cer(d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a neolactoside VI(3)-alpha-NeuNAc-nLc6Cer(d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80751"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:61610"/>
            <dbReference type="ChEBI" id="CHEBI:144452"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:80752"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.6" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013928675.1">
        <property type="nucleotide sequence ID" value="XM_014073200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYM3"/>
    <dbReference type="GeneID" id="106554512"/>
    <dbReference type="KEGG" id="tsr:106554512"/>
    <dbReference type="CTD" id="10402"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008118">
        <property type="term" value="F:N-acetyllactosaminide alpha-2,3-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000007">
        <property type="entry name" value="Type 2 lactosamine alpha-2,3-sialyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051142">
        <property type="entry name" value="Glycosyltransferase_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13713">
        <property type="entry name" value="SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13713:SF8">
        <property type="entry name" value="TYPE 2 LACTOSAMINE ALPHA-2,3-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013928675.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="40313" checksum="340CD655A4BD1778" modified="2020-10-07" version="1">MAALHFPVKARGRRRGDMLVMKQILFMMVLGAGVMYAVLHSNLWKTNSLGWSSLSRKRCNFKTNVSTFIQMDNFCPFLCESNFYDISARRGSDKFDLPYGVRNSELHFQIALSKLKSCDLFQEDDSSPSCKKCIVVGNGGILRNKKLGQKIDSYDVVIRMNNGPVIGYEDDVGRRTTFRLFYPESMFSDPLHYDPKTTAVLVVFKRHDLKWLSDLLSGNNIISTSAFWKKPAMRMIYKPNQIRILDPFIIKRTAYELLRFPRKFPKRLRPKHPTTGLIAIAFAFDICTEVHVTGFKYNLKDQGSSLHYYGNDTMSMMIKNEYHDINAEQRLLKNLIDHHIVINLTEEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZC3</accession>
    <name>A0A6I9YZC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">VPS35</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929226.1">
        <property type="nucleotide sequence ID" value="XM_014073751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZC3"/>
    <dbReference type="GeneID" id="106554990"/>
    <dbReference type="KEGG" id="tsr:106554990"/>
    <dbReference type="CTD" id="55737"/>
    <dbReference type="OrthoDB" id="10258141at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031748">
        <property type="term" value="F:D1 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099639">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903829">
        <property type="term" value="P:positive regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099175">
        <property type="term" value="P:regulation of postsynapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929226.1"/>
        </source>
    </evidence>
    <sequence length="797" mass="91853" checksum="25BAE8E122A9738B" modified="2020-10-07" version="1">MPTTSQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDDGEQIDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNANVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELMRLLKIPVDTYNNVLTVLKLKHFHPLFEYFDYESRKTMSCYVLSNVLDYNTEIISQDQVDAIMNLVSTLIQDQPDQPAEDPDPEDMADEQGLVGRFIHLLRSDDPNQQYLILNTARKHFGAGGNQRIRYTLPPLVFAAYQLAFRYKENEKVDDKWEKKCQKIFTFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFGEENHEPLRTQCALAASKLLKKPDQCRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENETVTIQVLNQLIQKIREDLPNLESTEETEQINKHFQNTLEHLRLRRESPESEGPIYEGLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z062</accession>
    <name>A0A6I9Z062_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 619</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF619</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930358.1">
        <property type="nucleotide sequence ID" value="XM_014074883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z062"/>
    <dbReference type="GeneID" id="106555942"/>
    <dbReference type="KEGG" id="tsr:106555942"/>
    <dbReference type="CTD" id="285267"/>
    <dbReference type="OrthoDB" id="9032483at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001009">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002196">
        <property type="entry name" value="zinc finger protein 850-like isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="121"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013930358.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="39755" checksum="5B94AC0239B1C753" modified="2020-10-07" version="1">MAPLRKSRIKLQGSHKPMLVSLVPRGSPQRRRTCPTRTVSFLQRKRGPEMQGLFVDASNDFPKIKKEAPSDAGQRQFFKQILENEDAVLQGNEKSLMRPPNMSTLVSRVGSASAESAMVPLSFEDVAVYFTEEECPLLNASQVALQLEVMVENYQNLTFIGREAWWNEAEDESQRVSLKRSRSIQEGLQSEGKEERVKVMRWDEFSLSERHPIQGATGAPPRYPGNNAPLAPASGNFCKTIQAKSSTRSISDSKTVDSPETPAQGQLAQKPRMNTCPICGKSFAYRSLLHEHERIHAKEKPFKCNECGISFSQMATLTSHKRIHTGEKPYQCMACGKSFSHSITLHSHRRSHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0Q3</accession>
    <name>A0A6I9Z0Q3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein argonaute-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106555771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930157.1">
        <property type="nucleotide sequence ID" value="XM_014074682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0Q3"/>
    <dbReference type="GeneID" id="106555771"/>
    <dbReference type="KEGG" id="tsr:106555771"/>
    <dbReference type="OrthoDB" id="10252740at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="1">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930157.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="28046" checksum="B044B0653790726E" modified="2020-10-07" version="1">MDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2G5</accession>
    <name>A0A6I9Z2G5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonuclease H</fullName>
            <ecNumber evidence="3">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NME8</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the NDK family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013930206.1">
        <property type="nucleotide sequence ID" value="XM_014074731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2G5"/>
    <dbReference type="GeneID" id="106555817"/>
    <dbReference type="KEGG" id="tsr:106555817"/>
    <dbReference type="CTD" id="51314"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902176">
        <property type="term" value="P:negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04416">
        <property type="entry name" value="NDPk_TX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46161">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46161:SF1">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="7">
        <location>
            <begin position="144"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="7">
        <location>
            <begin position="279"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="482"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="484"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013930206.1"/>
        </source>
    </evidence>
    <sequence length="618" mass="69756" checksum="CAD479024BB1B21D" modified="2020-10-07" version="1">PDFEDFVSYMLSAPSYILIISERKHGQRGEGDTVSPIVKHIKFKCCARGAARAVSSHGGSERNMGKHMTFTPPETLAGLAVANATALANLHGCSLLLQCLILKEMLQNQDLLHHCDIQDRVEETSRQLAFFFSDFSKQRNEKFVERTVALIRPSLLKERRESILNRIVENGFEIAMQKEIILSEEQAREFYREHENQDYFPYLLEQMTSGPTLALALVRENAIQKWRDLLGPKIIQEAKEQYPQSLRAEFAIHDSPINQLHGSANTNEAAKELQFFFPVENTMALIKPSAFEDKDKIINEVKNAGFLISELREANISPEIAAQFYQDHEDKPYYNQLVDYMSKGPSVVMVLTKENAVEEWRQLMGPTDPMKAKETHPSSIRAKFATDVMRNAIHGSSNEGHAKKSINFIFGDIDLESLKYKYIYISSIHSFNEWIVGSAVGSSGMGLKSWPVVTLKGVKPVLECTALLKATSAKVPQVCTPATCEKKGPRKPPEKGCLAHQPDKASPQAENLEKPPDGKSPPNSEEENSSKDDPMFRILPFGLQGVPSVFLQLINEVLHEHLYKEVLIYLDDILIYNEKHDEHVTLCEFHQERIDNLGYHISHKGIEMDPAKVQAVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2H8</accession>
    <name>A0A6I9Z2H8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KH homology domain-containing protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Brings lots of money 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KIAA0907</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">RNA-binding protein involved in pre-mRNA splicing. Interacts with the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome. Involved in regulating splice site selection. Binds preferentially RNA with A/C rich sequences and poly-C stretches.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KHDC4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930434.1">
        <property type="nucleotide sequence ID" value="XM_014074959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2H8"/>
    <dbReference type="GeneID" id="106556010"/>
    <dbReference type="KEGG" id="tsr:106556010"/>
    <dbReference type="CTD" id="22889"/>
    <dbReference type="OrthoDB" id="397265at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22385">
        <property type="entry name" value="KH-I_KHDC4_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000045">
        <property type="entry name" value="KH domain-containing 4, pre-mRNA-splicing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047890">
        <property type="entry name" value="KHDC4_KH-I_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056149">
        <property type="entry name" value="PRP5/DDX46/KHDC4_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031121">
        <property type="entry name" value="RIK/BLOM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15744">
        <property type="entry name" value="BLOM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15744:SF0">
        <property type="entry name" value="KH HOMOLOGY DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23469">
        <property type="entry name" value="KH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent RNA helicase PRP5/DDX46/KHDC4 KH" evidence="6">
        <location>
            <begin position="36"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930434.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="18304" checksum="A937410A9FA35181" modified="2020-10-07" version="1">MLMAKGKLKPSSNSADKLQAPGKGPSANKSKDDLVVAEVEINDVPLTCRNLLTRGQTQDEISKLSGAAVSTRGRYMTAEEKLKIGPGDRPLYLHVQGQTRELVDRAVNRIKEIITNGVVKAATGSSPTFNGATVTVYHQPAPITPLPPAVGQKTPFQSGGVSIARGGIFSSVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2J5</accession>
    <name>A0A6I9Z2J5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Neuroligin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106555721</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930090.1">
        <property type="nucleotide sequence ID" value="XM_014074615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2J5"/>
    <dbReference type="GeneID" id="106555721"/>
    <dbReference type="KEGG" id="tsr:106555721"/>
    <dbReference type="OrthoDB" id="6846267at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000379">
        <property type="entry name" value="Neuroligin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000906">
        <property type="entry name" value="Neuroligin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026972597" evidence="3">
        <location>
            <begin position="38"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="45"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930090.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="25944" checksum="5C7EFC8307B46F9C" modified="2020-10-07" version="1">MFLSSPSCVRRMLNVRPGSALLFWLLGLALRPLPISGQKPEDKYPVVPTNYGKLRGIKKGLNNEILGPVVQYLGVPYATPPVGERRFQPPEAPASWSEVRNATAFAPVCPQNIHGMLPAIMLPVWFTDNLEIVANYVQNQSEDCLYLNVYVPMEDGPLTKKREEASANNPSPDEDIRDSGKKPVMLFIHGGSYMEGTGNMFDGSVLAAYGNVIVVTLNYRLGVLGFMSTGDQSAKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2V0</accession>
    <name>A0A6I9Z2V0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dedicator of cytokinesis protein 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106556896</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931408.1">
        <property type="nucleotide sequence ID" value="XM_014075933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2V0"/>
    <dbReference type="GeneID" id="106556896"/>
    <dbReference type="KEGG" id="tsr:106556896"/>
    <dbReference type="OrthoDB" id="5844072at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF78">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013931408.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14216" checksum="E0F091F34FE9D0D6" modified="2020-10-07" version="1">MDTFTFVGTQLPCHFLTLLFLFHKVSSKLYSLPIPSTLVSLRLDFLRIVCSHEHYVTLNLPCSLLTPPASPSPSVSSATSQSSGFSTNVQDQKIANMFEFSVPFRQQHYLAGLVLTELAVILDPEADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z488</accession>
    <name>A0A6I9Z488_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein furry homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931039.1">
        <property type="nucleotide sequence ID" value="XM_014075564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z488"/>
    <dbReference type="GeneID" id="106556579"/>
    <dbReference type="KEGG" id="tsr:106556579"/>
    <dbReference type="OrthoDB" id="6287725at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045842">
        <property type="entry name" value="Fry_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19421">
        <property type="entry name" value="Fry_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein furry C-terminal" evidence="1">
        <location>
            <begin position="13"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931039.1"/>
        </source>
    </evidence>
    <sequence length="209" mass="23193" checksum="65AC994401627422" modified="2020-10-07" version="1">YFLCYSFGEGDRTASPQLSPFFSAILAAFQPTACDAAEEAWRSHINQLMCDSDGSCAVYTFHVFSSLFKNIQKRFCFLTCDAASYLGDNLRGIGSKFVSSSQMLTSCSECPTLFVDAETLLSCGLLEKLKFSVLELQEYLDTYNNRKEATLSWLANCKATFSGGSRDGVITCQPGDSEEKVIKNLGYMMVFLEEDLCVPCIDFNFSLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4E2</accession>
    <name>A0A6I9Z4E2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Unconventional myosin-Ib-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106556370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930841.1">
        <property type="nucleotide sequence ID" value="XM_014075366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4E2"/>
    <dbReference type="GeneID" id="106556370"/>
    <dbReference type="KEGG" id="tsr:106556370"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF26">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 5" id="KW-0518">Myosin</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="6">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930841.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="14787" checksum="67FD49F194834AD4" modified="2020-10-07" version="1">MAKMEVKTSLLDNMIGVGDMVLLEPLTEETFISNLKKRFDHNEIYTYIGSVVISINPYRLLPIYSADKVEEYRNRNFYELRPHIFAIFLYFLVPGNPRLRPDAPLDPSKRVGGKSGTAPGAAQLQLSALAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7I2</accession>
    <name>A0A6I9Z7I2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cytochrome P450 2K1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106557391</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932099.1">
        <property type="nucleotide sequence ID" value="XM_014076624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7I2"/>
    <dbReference type="GeneID" id="106557391"/>
    <dbReference type="KEGG" id="tsr:106557391"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF302">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013932099.1"/>
        </source>
    </evidence>
    <sequence length="274" mass="31664" checksum="5F4C62E0D6BD8054" modified="2020-10-07" version="1">MFPKLSFLSSTRKKLINNRKEFHDFIQTTFIEYLKNLDENDQRSFIDSFLIRQREDNKKMTNGYFHNENLKALSGNLFAAGTETTGSTLCWAILLMMKYPEIQRKVQEEIAKEIGVRQPRTEDRFKMPYTNAVIHEVQRFADIVPTNLPHATTMDITFKGFFIPKGMQIVPLLSSVLHDESQWEKPYKFYPEHFLDSEGKFMKRDAFMPFSAGKRICAGETLAKMELFLFFTSLLQKFTFQTPLGISKDDLVLTPATGFITSAIPYKTCAIPCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WY55</accession>
    <name>A0A6I9WY55_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphomevalonate kinase</fullName>
            <ecNumber evidence="3">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PMVK</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid mediated biosynthesis of isopentenyl diphosphate and other polyisoprenoid metabolites.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP</text>
            <dbReference type="Rhea" id="RHEA:16341"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:16342"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:16343"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013907081.1">
        <property type="nucleotide sequence ID" value="XM_014051606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WY55"/>
    <dbReference type="GeneID" id="106537468"/>
    <dbReference type="KEGG" id="tsr:106537468"/>
    <dbReference type="CTD" id="10654"/>
    <dbReference type="OrthoDB" id="2401875at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001026">
        <property type="entry name" value="Phosphomevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005919">
        <property type="entry name" value="Pmev_kin_anim"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01223">
        <property type="entry name" value="Pmev_kin_anim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13101">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13101:SF1">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04275">
        <property type="entry name" value="P-mevalo_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="6" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="15" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013907081.1"/>
        </source>
    </evidence>
    <sequence length="155" mass="18065" checksum="D5EF6F832EB8B77D" modified="2020-10-07" version="1">MARKATSSPLKEQYAKEHGLDFLRLLDATDYKELYREDMIRWGEERRQSDPGFFCRIVVEGVTQPIWIVSDTRRSSDVEWFRDVYGDIVQIVRVIATEETRTRRNWVFVAGIDDAESECGLDQGVPYDWVVTNDGDQLSLDAQLEKLLQFIQTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZU8</accession>
    <name>A0A6I9WZU8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neuronal membrane glycoprotein M6-b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GPM6B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the myelin proteolipid protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907646.1">
        <property type="nucleotide sequence ID" value="XM_014052171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZU8"/>
    <dbReference type="GeneID" id="106537890"/>
    <dbReference type="KEGG" id="tsr:106537890"/>
    <dbReference type="CTD" id="2824"/>
    <dbReference type="OrthoDB" id="9993736at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043209">
        <property type="term" value="C:myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022010">
        <property type="term" value="P:central nervous system myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051612">
        <property type="term" value="P:negative regulation of serotonin uptake"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001614">
        <property type="entry name" value="Myelin_PLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018237">
        <property type="entry name" value="Myelin_PLP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11683">
        <property type="entry name" value="MYELIN PROTEOLIPID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11683:SF10">
        <property type="entry name" value="NEURONAL MEMBRANE GLYCOPROTEIN M6-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01275">
        <property type="entry name" value="Myelin_PLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00214">
        <property type="entry name" value="MYELINPLP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00002">
        <property type="entry name" value="PLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00575">
        <property type="entry name" value="MYELIN_PLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01004">
        <property type="entry name" value="MYELIN_PLP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013907646.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="31262" checksum="8A1E83FE0D154B26" modified="2020-10-07" version="1">METAVEENREQRQEKKGCFECCIKCLGGVPYASLVATILCFSGVALFCGCGHVALAGTISILEQNFSTNVTDHALLSEVIKLMQYVIYGIASFFFLYGIILLAEGFYTTSAVKELHGEFKTTICGRCISGMFVFLTYVLGVAWLGVFGFSAVPVFMFYNMWSTCEVIRSLSANMTSADQICVDIRQYGIIPWNAIPGKACGSTLANMCSTPEFYLSYHLFIVACAGAGATVIALIHFLMILSSNWAYLKDASKMQAYQDIKAKEEQELQDIQSRSKEQLNSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3E4</accession>
    <name>A0A6I9X3E4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">COPB2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.</text>
    </comment>
    <comment type="function">
        <text evidence="14">This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasmic vesicle</location>
            <location evidence="1 15">COPI-coated vesicle membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
            <orientation evidence="15">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="15">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908826.1">
        <property type="nucleotide sequence ID" value="XM_014053351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3E4"/>
    <dbReference type="GeneID" id="106538748"/>
    <dbReference type="KEGG" id="tsr:106538748"/>
    <dbReference type="CTD" id="9276"/>
    <dbReference type="OrthoDB" id="2150324at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000008">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 15" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <keyword evidence="6 16" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="11"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="181"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="225"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="17">
        <location>
            <begin position="319"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="18">
        <location>
            <begin position="594"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013908826.1"/>
        </source>
    </evidence>
    <sequence length="910" mass="102910" checksum="248E2CD4BBD9CE2B" modified="2020-10-07" version="1">MPLRLDIKRKLTARSDRVKSVDLHPTEPWMLASLYNGSVCVWNHETQTLVKTFEVCDLPVRAAKFVARKNWVVTGADDMQIKVFNYNTLERVHMFEAHSDYIRCIAVHPTQPFILTSSDDMLIKLWDWDKKWACSQVFEGHTHYVMQIVINPKDNNQFASTSLDRTIKVWQLGSSSPNFTLEGHEKGVNSIDYYSGGDKPYLISGADDRLVKIWDYQNKTCVQILEGHAQNVSCVSFHPELPIIITGSEDGTVRIWHSSTYRLESTLNYGMERVWCVASLRGSNNVALGYDEGSIIVKLGREEPAMSMDANGKIIWAKHSEIQQANLKAMGDTEIKDGERLPLAVKDMGSCEIYPQTIQHNPNGRFVVVCGDGEYIIYTAMALRNKSFGSAQEFAWAHDSSEYAIRESNSSVKIFKNFKEKKSFKPDFGAEGIYGGFLLGVRSVNGLAFYDWENTELIRRIEIQPKHIFWSDSGELVCIATEESFFILKYISEKVATAQDTHEGVTEDGIEDAFEVLGEIQEIVKTGLWVGDCFIYTSSVNRLNYYVGGEIVTIAHLDRTMYLLGYIPKDNRLYLGDKELNIVSYSLLVSVLEYQTAVMRRDFSMADKVLPTIPKEQRTRVAHFLEKQGFKQQALAVSTDPEHRFELALQLGELKIAYQLALEAESEQKWKQLAELAINKCQFSLAQECLHHAQDYGGLLLLATASGNTSMVNKLAEAAEKDGKNNVAFMSYFLQGELDSCLELLIKTGRLPEAAFLARTYLPSQVSRVVKLWRENLSKVNQKAAESLADPTEYENLFPGLKEAFVAEEYIKNSQTTSRPASEYPLVTPNEERNILEEAKDFTLQNKISEEPEDESVPSPRPVITPPVAGKISLDIKDEKVLLDLDVDLDNLELEDIDTTDIFLDEEMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X461</accession>
    <name>A0A6I9X461_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein odd-skipped-related 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">OSR1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Odd C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910109.1">
        <property type="nucleotide sequence ID" value="XM_014054634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X461"/>
    <dbReference type="GeneID" id="106539756"/>
    <dbReference type="KEGG" id="tsr:106539756"/>
    <dbReference type="CTD" id="130497"/>
    <dbReference type="OrthoDB" id="9451254at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001655">
        <property type="term" value="P:urogenital system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000254">
        <property type="entry name" value="Odd-skipped related transciption factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000090">
        <property type="entry name" value="Odd-skipped-related transciption factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000311">
        <property type="entry name" value="protein odd-skipped-related 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF5">
        <property type="entry name" value="PROTEIN ODD-SKIPPED-RELATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013910109.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="29330" checksum="86C936D848C8AA59" modified="2020-10-07" version="1">MGSKTLPAPVPIHPSLQLTNYSFLQAFNGLPAVPSDHLPNLYGFSALHAVHLHQWTLGYPAMHLPRSSFSKVPGVASLVDARFQLPAFPLFPHVIHPKHEAANVPLKAKPRFDFANLAVAATQEDHSKLGQIDNQGSSAGLGPLLEVTKLSPEKKPTRGRLPSKTKKEFVCKFCGRHFTKSYNLLIHERTHTDERPYTCDICHKAFRRQDHLRDHRYIHSKEKPFKCQECGKGFCQSRTLAVHKTLHTQVKELRPSKMKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6N7</accession>
    <name>A0A6I9X6N7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WAP four-disulfide core domain protein 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106539621</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909921.1">
        <property type="nucleotide sequence ID" value="XM_014054446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6N7"/>
    <dbReference type="GeneID" id="106539621"/>
    <dbReference type="KEGG" id="tsr:106539621"/>
    <dbReference type="OrthoDB" id="9048113at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051388">
        <property type="entry name" value="Serpin_venom_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46751">
        <property type="entry name" value="EPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46751:SF1">
        <property type="entry name" value="WAP FOUR-DISULFIDE CORE DOMAIN PROTEIN 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00003">
        <property type="entry name" value="4DISULPHCORE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026704471" evidence="2">
        <location>
            <begin position="19"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="4">
        <location>
            <begin position="116"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="4">
        <location>
            <begin position="217"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="3">
        <location>
            <begin position="270"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="3">
        <location>
            <begin position="328"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013909921.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="43215" checksum="60B95CCD8FA465BE" modified="2020-10-07" version="1">MKGPLVCFLLLAATFAAATEWEEVPPTYGECPAPTHVAPNAYYCMANRPCPGDEKCCQVHNIMKCVLPKGVHQGYCPRPQDQAVYKKPCAGDHQCGWHEKCCLKDGQKKCVQAVPDIKKPGTCPPPVQDEDKRCGKYCSSDAKCPGIERCCPTSCGQECRIPQRYMPGYCPAVLLPPSRGDVCFRTCTNDFDCNHGIQLPLKKCCDFEGRKICVDNMEEHPGVCLRRVEVQPFVPCNDTCDDDQDCPLTEKCCFTGCGRGCLPSVRSEVCQLPMAQGSCKKSLTRFYYDPEKKKCVSFIYRDCEGNRNNFETKELCEKACGEISKEVCRLPKETGPCQGFSMHYYHNWETKKCEMFAYGLCGGNENRFSTKLECEMVCGGFGKEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6X7</accession>
    <name>A0A6I9X6X7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PDIK1L</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013910006.1">
        <property type="nucleotide sequence ID" value="XM_014054531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6X7"/>
    <dbReference type="GeneID" id="106539685"/>
    <dbReference type="KEGG" id="tsr:106539685"/>
    <dbReference type="CTD" id="149420"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13977">
        <property type="entry name" value="STKc_PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000174">
        <property type="entry name" value="Serine/threonine-protein kinase PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000165">
        <property type="entry name" value="Serine/threonine-protein kinase PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF58">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="8"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910006.1"/>
        </source>
    </evidence>
    <sequence length="341" mass="38457" checksum="07628937E0D0E397" modified="2020-10-07" version="1">MVSSQPKYDLIREVGHGSYGVVYEAVVRKTSARVAVKKIRCHAPENVELALREFWALSSIKSQHPNVIHLEECILQKDGMVQKMSHGSSSSLYLQLVETSLKGEIAFDPRSAYYLWFVMDFCDGGDMNEYLLSRKPSRKTNTSFMLQLSSALAFLHKNQIIHRDLKPDNILISQSRMDASDLEPTLKVADFGLSKVCSASGQNPEEPVNVNKCFLSTACGTDFYMAPEVWEGHYTAKADIFALGIIIWAMLERITFVDTETKKELLGSYVKQGTVIVPVGEALLENPKMELLIPVKKKKMNARMKQLIKEMLAANPQDRPDAFELELRLVKIAFKDSSWDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAQ4</accession>
    <name>A0A6I9XAQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Junctional adhesion molecule B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">JAM2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912684.1">
        <property type="nucleotide sequence ID" value="XM_014057209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAQ4"/>
    <dbReference type="GeneID" id="106541694"/>
    <dbReference type="KEGG" id="tsr:106541694"/>
    <dbReference type="CTD" id="58494"/>
    <dbReference type="OrthoDB" id="10015491at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098636">
        <property type="term" value="C:protein complex involved in cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007159">
        <property type="term" value="P:leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000342">
        <property type="entry name" value="Junctional adhesion molecule A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042625">
        <property type="entry name" value="JAM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44663">
        <property type="entry name" value="JUNCTIONAL ADHESION MOLECULE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44663:SF2">
        <property type="entry name" value="JUNCTIONAL ADHESION MOLECULE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="71"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013912684.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="26305" checksum="C029B805892D7099" modified="2020-10-07" version="1">MIKGSTLSFVYLRGAFVGNFKDRAEILHSGIHIKNVTRKDSAKYRCEISAPTEQGQGTGEVEISLLVLVPPSAPACEVPISALSGTVVELRCKESEGVPASKYRWYRNGAVLPENTFFWRFRKEKNSYTLNTTTGTLLFNPVSKNDTGEYYCEAYNRVGRARRCPVKRMQVDDLNVSAIIASVVIVALIIASCGLGVYYAQKKGYFLKRNSSMNNSSKYRATESGKDFKHTKSFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBR7</accession>
    <name>A0A6I9XBR7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transforming acidic coiled-coil-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913019.1">
        <property type="nucleotide sequence ID" value="XM_014057544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBR7"/>
    <dbReference type="GeneID" id="106541957"/>
    <dbReference type="KEGG" id="tsr:106541957"/>
    <dbReference type="OrthoDB" id="10255048at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039915">
        <property type="entry name" value="TACC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL CONTAINING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924:SF12">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="563"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913019.1"/>
        </source>
    </evidence>
    <sequence length="585" mass="62615" checksum="C620CC16279FC7FE" modified="2020-10-07" version="1">MGGLQSRAEPRPPPALLPPGAQAEQSPRGRLEGVPGSDSEGNFGTPEAETPIHSLGKELEELDLELDVEPQASLEDQFVSKVLEETSASAEDELQTDSADVQASDAFISKAHLGIEQGSSVSSSPLEATEENTVPARLNPDHTSGTPSREEQRDILKESQDIPIQEPSEVKSSVENIASNKSKLPRAKPIALQKKMGGENSEAEVTTESPPIPKATYHFDPEEYNETVNPFRMGGSKCPNSPPASRPSADLPHSDPKHPSSAGAGDCKTRAVKLEFDFAEEAENAEPKKLPARKMGKRPSSKLSPKRQRATRPVVGPEDGEKTIATETSVAPLTRATPLADTNQWDDPAFNPFVGSSSLQDSPTLTKRSYHLDVGDPFKPGSKTLSTVGADSCPSAENSLNEILESQAREAAGDDLKTSLAPQSSRSRLITTTEQVKFLCFLLSGCKVRKYETHSLALDSCAQDEEEAALISKIPDLPSRDGHATDEEKLASTGQKPAELEVKGEPEEDLEYFECSNVTVSTINHTFSSPVEGSDKESLCRLEEKNNSSDISSNPELCSMEKAPGSAPGSPRGGAESPLDAICLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCK1</accession>
    <name>A0A6I9XCK1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transmembrane and coiled-coil domains protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMCC2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEX28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911582.1">
        <property type="nucleotide sequence ID" value="XM_014056107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCK1"/>
    <dbReference type="GeneID" id="106540843"/>
    <dbReference type="KEGG" id="tsr:106540843"/>
    <dbReference type="CTD" id="9911"/>
    <dbReference type="OrthoDB" id="10072335at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042982">
        <property type="term" value="P:amyloid precursor protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019394">
        <property type="entry name" value="TEX28/TMCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17613">
        <property type="entry name" value="CEREBRAL PROTEIN-11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17613:SF9">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAINS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10267">
        <property type="entry name" value="Tmemb_cc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="629"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="659"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="147"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="432"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="506"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013911582.1"/>
        </source>
    </evidence>
    <sequence length="692" mass="76974" checksum="D5C2C5E3FB5DFC5A" modified="2020-10-07" version="1">MKRCKSDELQQLDGDSAGTEDALLLPTMDAKLGDSAAAPVDLGVSNPNPAPHPATRSKPPDLKKIQQLSEGSMFGHGLKHLFHSRRRSREREHQNSQESSQQHHGMSDHDSPDEKERSPEMHRVSYAVSLHDLPARPTAFNRVLQQIRSRPSIKRGTSLHSGSRRAKSSSVEPQKSSPHLVRRAPQDSSLTAILHQHQGRPRSSSTTDTTILMAESGNVYFLTEESECVTDKLDKGDVSQLNLPSNTSHSDAESNICLDVPDGNPDPQRTKAAIDHLHQKILKITEQIKIEQEARDDNVAEYLKLANNADKQQASRIKQVFEKKNQKSAQTIAQLHKKLEHYHKKLKEIEQNGPTRQPKDVFRDMHQGLKDVGANVRSSISGFGGGVVEGVKGGLSGLSQATHTAVVSKPREFASLIRNKFGSADNIAHLKDTLDDGHPEEASRTLSGSATLVSSPKYGSDDECSSATSGSAAGSNSGAGAGGLGSPKSNTLESHHYNFDTIVEELRDIKDIQSHLEDSIEELKAQLQRDYTYMTQCLQEERYRYERLEEQLNDLTELHQNEMTNLKQELASMEEKVAYQSYERARDIQEAVESCLTRVTKLELQQQQQQVVQLEGVENANARALLGKFINVILALMAVLLVFVSTIASFITPMVKTRMRLLSTTLLVLFVFLLWKHWDTITYLLEHVLLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCY7</accession>
    <name>A0A6I9XCY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TTC3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013911712.1">
        <property type="nucleotide sequence ID" value="XM_014056237.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540932"/>
    <dbReference type="KEGG" id="tsr:106540932"/>
    <dbReference type="CTD" id="7267"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000143">
        <property type="entry name" value="E3 ubiquitin-protein ligase TTC3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056872">
        <property type="entry name" value="TTC3/DZIP3-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056870">
        <property type="entry name" value="TTC3/DZIP3/RBM44-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043866">
        <property type="entry name" value="TTC3/DZIP3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056871">
        <property type="entry name" value="WH_TTC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17550">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17550:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24525">
        <property type="entry name" value="TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24905">
        <property type="entry name" value="TTC3_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19179">
        <property type="entry name" value="TTC3_DZIP3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24812">
        <property type="entry name" value="WHD_TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase TTC3/DZIP3" evidence="10">
        <location>
            <begin position="604"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase TTC3 winged helix turn helix" evidence="12">
        <location>
            <begin position="1046"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="domain" description="TTC3/DZIP3-like helical" evidence="11">
        <location>
            <begin position="1385"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="domain" description="TTC3/DZIP3/RBM44-like helical" evidence="13">
        <location>
            <begin position="1758"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="358"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1673"/>
            <end position="1696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1811"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1391"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="419"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1673"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1812"/>
            <end position="1846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF24905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013911712.1"/>
        </source>
    </evidence>
    <sequence length="2018" mass="233231" checksum="7AD2C1C7DCC0EADC" modified="2020-10-07" version="1">MIQGIRGLRSRSMENPTFEEIRYISLYSPYIRPCCELMRGTFHEYSPKKHEQGDICQMWCSKPVSQLQKYCDIIKICTFRPLLFIPHRDPTKKCHDPSCQFDELNLEKLSQIEILEDVTDIVKKFADDQLFINGILTTETDLEKGILRTSEVLEWMKYADHIGILQKLRKLADYCCWSILKLFFSAYKYYISKVTLEDYNLVEAFESYYCDNCMMNSENMKKRGNEAFAKDKFDSAVTFYTKAIELWPENHFLYGNRALCFLRIGQYRKALCDGKRAIILKPSWPKGHYRFCDALSFLGEHRKALEANERGQVLCRENPEGVKGLIEQHEKLKNQMEDARGIKQNKYRLKKQLLQKNSSEYVSVGNQESKKSHNENKKLCDSHHHPSQQKQTNMVGSIKTRVGRENIDHLSVFPAEVSENPKKQRSQMDDSEKLKEQVNLKTDSKKVVEKHNLQCGNVQQGEVIPSLEMLKTYMKNGSTSLIDQCFHSAEQSFALSLNMVDPSQLQCLNLALVDYVVIMYGYASALLGIGQPEELSKAKYHFDKIIEQYQKVRFNCLAHYGIGKVYLRQNRFSEALDQFMKSKTMVNLKIVPGVLTWPTTSVIIKETRSEALQVILEDCITECKFSPKPNAVCRYQQCQAHKVKIYFNDPVFKGFIRIACCKQCIVEFHLSCWKKVKATRYCDKNDKDILQETCFTPDCSGLISKITVFSSSGIVKCEFEHKIITKNPPRPVLKQKCSSFRKLQVKEEKKLRRKFIKEAVSFRNLDVFHRSNIVAKDDSFKENIPKNFFVEDTILQLIGRYKMTIQDGVHDAFKLLVELLSWWVLSEEDYALFSTSRLSSAEIMDQLISFLIAKNDRVKTRIFVHVVSAFEEVDPKLHDWMKHLDNNGLKATEFFFSEYFTYILLIDFKSVAVLWNEKYGIKLSRTFTCLSEDVHEIITCLEKLSLMELRCLVWLLEENRKCFPFLHQCLDDYFDNLDNPFIVLRKEYTETTSNNAIKVKNKNRKKSKESKAILVVSGGVGTVAQEEDNIFSEENTLSFMNPHEPFRIPDDLQEQVMMFEALYNTASSNNSYQRILDNYPDPTCESLYDYFSQILEEHGPMEIDNPLLVGEYEDFPTDTRKIVENAGGLKSFLLGSLRFVMVDDLIGLMKHAIMLQENTEVIEMDENNYSESPYCHENNFHSISSLNPTAKEFKPVSFINKPYISTSTDNLEYVTISQSYSPFASSYSFSSQTDTAPAVSDNHTIFMNEIDSEYVNERLSPVPFMPDISEETNYIYIDCNTYLDADSEATHNKCNVDNFTTVDQVHTFQSSPCITVKSDIHTCTKNLDQTEDHGCNDAECSSVTKIKTENKYLARNNPRSRMIAIQVDQEQTDAGVNTLPLHPYESQQGDMLRMEKEYHVLQKQLKEAIEKYEQLKRRSSKETSILEEELSLLIEGNKLSKRELDWFHQDAEVNFKKWQQEKKEKQEGIKTGKHKVRKLIETNETYMKIIDEKDKQYKAYLDEFLEISNRFECEKIKMEEAIKKTQDYQQECVKRAIAAEVSVLENWKEKEFYKLSRKSTNAKANLKYLKFMTSHSATPQSKLQIDSWESFISNIGEEKKKIESQFEERIYVVKNGDLLNRITPVETGDLQPPTGLSLATLEKSPINDPAIIMCSAAAPQSAPFSHVNDNYSLSSKAKTHTGNKNQEAPSDKDIGELPAECRQSSLSDKVCLPQSLGNSGRRLQNIQPLHSILEERARAAELDSATILNKPILRKLSENIIDQLRIIFPYHTSSDLENFIKEVNVKNRNKLSTDEYLNRVTEFILDHPNTKKVLSSSGKNEKLSSCTSGENGSHTQKVLNTLIQNKPKSKAANEKKNKTRLPPQSNQMPWKNIGETSKSKWKKSNDAIDNDPCVICHEELNSNLLHVLDCGHRFHKLTGGIAVCLEVAEKAAHRVRIQRFKPRNCNCETRTWCRKLFEHNLKKESLKRITKMMYMIWRMVVQPSCKISGLRNLLKHCENSVHYLEGDANYLRKKSWHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFT7</accession>
    <name>A0A6I9XFT7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch-like protein 34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KLHL34</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912737.1">
        <property type="nucleotide sequence ID" value="XM_014057262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFT7"/>
    <dbReference type="GeneID" id="106541737"/>
    <dbReference type="KEGG" id="tsr:106541737"/>
    <dbReference type="CTD" id="257240"/>
    <dbReference type="OrthoDB" id="10027872at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF8">
        <property type="entry name" value="KELCH-LIKE PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="29"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912737.1"/>
        </source>
    </evidence>
    <sequence length="582" mass="67041" checksum="3E2186A30B5B0177" modified="2020-10-07" version="1">MTYFLSYCKKHCSAVLSEYQTLRKEGLLCDILLKVKENEFPAHKSLLVCSSDYFKAMFKSYTKESKSAVVHLQVVSATGLQHVLDFIYTSWLPLSFTTLEDTLEAATYLQVPEAICLCSQYLGNNLNLENCCFSANIATKFYLPDALSAAEKYLIINLWKLLDLDFAGLLELNFRSLKAVVESPDILMVRESNLLDLLLKWLAYDKLRLIHTTNLLEHVRYSLIPMEDLRNIYTQSEVPLTTPVKCLIRKAINYHSLIYKQPILQDKYSTLRNQKIWIILLGGMIAHEGLVKEVMAFDVYNHKWQNLTELPDRIQNHCVCTIGNFLYVLGGEMEDMQSDHILKVTNKVHRYDPRFNKWIQITGMLEKRCQFSCCILENAIIAIGGQSEDKKLHSSVEIYDISKDRWTKLHDLPCKVHGHAGTVCKNTIYISGGKYMGQSNTSNDMYALSNLEGEWRKQAPMSIARFGHQMATIRDSIFTFLGFYEPFSEIEKYDPDQNQWTHLRPLVYDRYSYGLVLVEETAILIGGKKWQDSQEIPTQDVVGYDIDNDCWEVICKTPFPWYGLQCGVLELSELVEGKDMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG97</accession>
    <name>A0A6I9XG97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vertnin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">VRTN</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the vertnin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914694.1">
        <property type="nucleotide sequence ID" value="XM_014059219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG97"/>
    <dbReference type="GeneID" id="106543250"/>
    <dbReference type="KEGG" id="tsr:106543250"/>
    <dbReference type="CTD" id="55237"/>
    <dbReference type="OrthoDB" id="9869831at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22791">
        <property type="entry name" value="OTU_VRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038822">
        <property type="entry name" value="Vertnin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047273">
        <property type="entry name" value="VRTN_OTU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16081">
        <property type="entry name" value="VERTNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16081:SF0">
        <property type="entry name" value="VERTNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="369"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914694.1"/>
        </source>
    </evidence>
    <sequence length="674" mass="77766" checksum="682A5ADDF123AA5A" modified="2020-10-07" version="1">MIQRQQLLQSVLQKLQKASEYDGIKQLIKAALKCQMILSSFSLPSYYGRQFQEELEVDRVAKSLYPEDAPRNMLPFICKGNGDHLFEAASVLLWGNTSLTLELQVRTVVEMLLNKQYYFHGMIDSKVMLQAARYSLCTDKSPQMTKLPIATIEAIFDVDIKASCFIGSYVNMWHLYGLASVLQCNIYSIYPMSNLKIRPYFNRLIRPRTCNLQTSTLHIMWSGRQISSQVFEAQYFVPVMGLEELEPTASIPEAQVQPVKTLELLNKDPQVTYSNLRERYSITKSTFYRWKRQSREHRQKAAARFAAKHFLQSCFQEGNTIPLQHFRQLFPEISRSTYYAWKHEMQSVNNREVSSSSDVALSKDPQMYKLHTSPDGASTSEGGQSSNDFLNHPKNGTSMQGAKSYLEKCIAMNSLVPYRNFKRSFPGISRSTYYNWRRKAIKGNPNFKPPLPLSVIQKPTRNLYLSLKSGNLPNVKMVNGQHPGPRILMGTRQSLYSWQKQARELARKRVCQWNMPLCKFRLRYPNISSATYWFWRKKSVKETKKHCLPLTMASQNLQPTPTSSSEMKRKAELKFHSPHPEEFSQVLEDEQMPTCSTASPGYQMPETVNSSMFVMDVIATAQFKAQAKLFLQQRFASQTFPTFKEFRAHFPLTARSTYYMWKRALHDGLTLVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGP1</accession>
    <name>A0A6I9XGP1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">F-box/WD repeat-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FBXW5</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913032.1">
        <property type="nucleotide sequence ID" value="XM_014057557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGP1"/>
    <dbReference type="GeneID" id="106541962"/>
    <dbReference type="KEGG" id="tsr:106541962"/>
    <dbReference type="CTD" id="54461"/>
    <dbReference type="OrthoDB" id="192402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22132">
        <property type="entry name" value="F-box_FBXW5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000305">
        <property type="entry name" value="F-box/WD repeat-containing protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042508">
        <property type="entry name" value="FBXW5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20995">
        <property type="entry name" value="F-BOX/WD REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20995:SF17">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="511"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913032.1"/>
        </source>
    </evidence>
    <sequence length="572" mass="64872" checksum="9B7DA438510C35D9" modified="2020-10-07" version="1">MLPDSLLVEIFRHLPPPDVLAAGRVCRQWRRVARDEVLWKGLFYRHYGVARNVPRHPAAASWFGEFQRLGDAVPRVEVQRLREHGDPVLHLSFAHSGRLFASCSKDCTVKIWRNELPVSLLHSSSMKKYNWSYTQFSQFNADDSLLLVSGVFMGPRSSSSGEIAVIGLENFELLSRVRNKPFDVFGCWLNETNLISSNLHRIGYLTSCSVLWLNNAFQGIESENVNVVKRLFKIQNLNASAIRTVLVVDCGRYSSPEELESPGEQVEEGPAPPPQESPSQEPEERPQRRGFPAAEGEAGTEDGLDRFLSDIMAGHVRPMMTEMEMETKVARLLARNRTKPPEPNLLSMQGGNKKYLVFTTGCLTYSPHQIGIKQILPHQMTTAGPVLGEERNSDRFFDSLDHVIDIHGHIIGMGLSPDHRYLYVNSRAWPEGCVVSDPLQPPPIAEEIELRVVDLKTMKEAKRALRAHRAYTPSEEFNFIFLDVSKDFVASGAEDRHGYIWDRHYDICLAKLQHDDVVNSVAFSPVEQELLLTACADNTIKVWRSPRTARVLQDEKPPLRRRLFSWATKQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGR3</accession>
    <name>A0A6I9XGR3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Caspase-3</fullName>
            <ecNumber evidence="13">3.4.22.56</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 21 22" type="primary">CASP3</name>
    </gene>
    <organism evidence="19 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.</text>
            <dbReference type="EC" id="3.4.22.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.56" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013910221.1">
        <property type="nucleotide sequence ID" value="XM_014054746.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910222.1">
        <property type="nucleotide sequence ID" value="XM_014054747.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910223.1">
        <property type="nucleotide sequence ID" value="XM_014054748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGR3"/>
    <dbReference type="GeneID" id="106539846"/>
    <dbReference type="KEGG" id="tsr:106539846"/>
    <dbReference type="CTD" id="836"/>
    <dbReference type="OrthoDB" id="6116485at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031264">
        <property type="term" value="C:death-inducing signaling complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097194">
        <property type="term" value="P:execution phase of apoptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030216">
        <property type="term" value="P:keratinocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1470:FF:000002">
        <property type="entry name" value="Caspase-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000001">
        <property type="entry name" value="Caspase-3 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1470">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454">
        <property type="entry name" value="CASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454:SF198">
        <property type="entry name" value="CASPASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Caspase family p20" evidence="18">
        <location>
            <begin position="68"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="17">
        <location>
            <begin position="207"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013910221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013910222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013910223.1"/>
        </source>
    </evidence>
    <sequence length="300" mass="33787" checksum="1A1219B656EF254D" modified="2020-10-07" version="1">MEEETSFHSLTKGKQKTTMADKNHVLKSPQSVTDAKTLSGSEGKSIPFSKSVDSVIQADESYRMDCPEMGICILVNNKHFLPDTGMSLRSGTDADAALVLDTFRTLGYNVKIYNDLSCKEIIDVLQNISKDDHRKRNSFVCVLLSHGEDGLIYGTDGPIELKMLASLFRGDRCKTLRGKPKLFFIQACRGTDLDSGIETDSGSEERMCQKIPVEADFLYAYSTAPGYYSWRNSLDGSWFIQSLCVMLKQYAKKLELMQILTRVNCKVAEFSSYSNRPDFHGKKQIPCIVSMLTKDFYFPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI03</accession>
    <name>A0A6I9XI03_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pyridoxine-5'-phosphate oxidase</fullName>
            <ecNumber evidence="7">1.4.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Pyridoxamine-phosphate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PNPO</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>pyridoxamine 5'-phosphate + O2 + H2O = pyridoxal 5'-phosphate + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15817"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15818"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:15149"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:15150"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the pyridoxamine 5'-phosphate oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013913532.1">
        <property type="nucleotide sequence ID" value="XM_014058057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI03"/>
    <dbReference type="GeneID" id="106542350"/>
    <dbReference type="KEGG" id="tsr:106542350"/>
    <dbReference type="CTD" id="55163"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.110.10:FF:000020">
        <property type="entry name" value="PNPO isoform 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004231">
        <property type="entry name" value="PRK05679.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851:SF0">
        <property type="entry name" value="PYRIDOXINE-5'-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PYRIDOXINE-5-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000190">
        <property type="entry name" value="Pyd_amn-ph_oxd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="16">
        <location>
            <begin position="75"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridoxine 5'-phosphate oxidase dimerisation C-terminal" evidence="17">
        <location>
            <begin position="205"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF10590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013913532.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="30205" checksum="5DA2E62C11531E2F" modified="2020-10-07" version="1">MFRFRGLLWLRALNSTIPRSSGQVQKSSHAMDLGSLRKNYRGDEEAFEEKHLTSLNPIQQFSAWFEQAMKCPSIGEVNAMCLATCTREGRPSARMLLLKGFNQEGFRFFTNHESRKGRELDSNPFASMVFYWEPLNRQVRIEGSVERLPEQESEKYFHSRPKSSQIGAVVSRQSTVIADREYLRKKNAELEEIYRDTKVPKPGYWGGYILKPEVIEFWQGQTNRLHDRIVFRYLQDSSTSLDPLTHRGEGNWVYERLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIN1</accession>
    <name>A0A6I9XIN1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WAS/WASL-interacting protein family member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">WIPF1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913906.1">
        <property type="nucleotide sequence ID" value="XM_014058431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIN1"/>
    <dbReference type="GeneID" id="106542637"/>
    <dbReference type="KEGG" id="tsr:106542637"/>
    <dbReference type="CTD" id="7456"/>
    <dbReference type="OrthoDB" id="5877983at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd22076">
        <property type="entry name" value="WH2_WAS_WASL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47091:SF2">
        <property type="entry name" value="ALPHA-PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47091">
        <property type="entry name" value="ALPHA-PROTEIN KINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="488"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913906.1"/>
        </source>
    </evidence>
    <sequence length="955" mass="101991" checksum="566E20E79A42B60F" modified="2020-10-07" version="1">MENNNVPSLKRNDSFIPRTTQSVSDLFIYDGVEECWDFIEGKVDGFDSSQVDSTLASQIPERTDSCCFNAKALTACQTTEYAKTCEKGSLGKVLKKDILSESHTRVPLEDKFYLAENSSFLVDSGIEPQLDGNFIEVTEGFQNDFLKDTESSDATTEEIHEDWETKLKFLLESDDEEDELIPSTDCDGCSYFLGEMPRLCQVSDNTMPMETTIGFRRHPSKSKEVAVGSNLTAYRPSTLQTGMTLTVGQRQNMNPGMKIKEKQKELVIKKNYSRTGEENRGHSCPSCHVSPFGLQGIENGTSAMKSPSRHLDVSSATAEEQDTAECKSFSGEIKSRRVIQVGKKTMRGKSGLPHSSEDPSKALPPKPLHDSKLHTVQRETNHPGSVTSLHGQGKTTLGLTNVADKDALFHGQGSQPRDGLVGKWITGPSEGSQAAEENAMPKAVLMESMQTRKKYSDFIASVLVLIFCIFTWQANIEKPSLNRSEQSGRNALLSDITKGKKLKKAVTNDRSAPVFDKPKGPAGGGGGSSGFSSGSSGSGSSGNSYGGGGPPGLGGLFQAGMPKLRPTNRDNDSAGNKAPVFPPGGRATSAKPFSPPSGPGRFPGPSGLRAPATEPQRNRMPPSRPDFGSRPDTGAPPVPNTPRPIPSSLYNRGPPPVPGASRQASIGLTPPPFPGNRNSGPGGSIRQSSPVSPPSPFSNRPPLPPVPGRPTEDKTPPPLPPSGNRPSVSREASLPPPPPQNHKPPIPSTPRPPAISSVPPPLPPSRPGPPPVPPASGGNDEMPRLPQRNTSLVPCMPCPNRSGPPLPPPVERPPPPIRDPPSRSGPLPPPPPPLIRNGSTSRALPATPQLPSRGGFEKTRSGPVPPPPPDRPGSGAPPPPPPSLPSSALRNGFQDSSCDDEWESRFSFHPLSDLPPPEPYVPMNKSYPSKLVKNDNRSGSSRRERGAPPLPPIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ03</accession>
    <name>A0A6I9XJ03_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vesicle transport through interaction with t-SNAREs homolog 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">VTI1A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915739.1">
        <property type="nucleotide sequence ID" value="XM_014060264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJ03"/>
    <dbReference type="GeneID" id="106544108"/>
    <dbReference type="KEGG" id="tsr:106544108"/>
    <dbReference type="CTD" id="143187"/>
    <dbReference type="OrthoDB" id="430637at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013915739.1"/>
        </source>
    </evidence>
    <sequence length="127" mass="14545" checksum="83092C49F1BCF1AB" modified="2020-10-07" version="1">MTKLLDISSIKYPLEGSRGFEFSNLDSDSSRALLAHISLFFFFLLQKRSRIAYSDEVRNELLGDDGNSSEIQLIKLREERAHLLDNTERLERSSRRLEAGYQIAVETGKNSESEQIVLLMHSSLHNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XL48</accession>
    <name>A0A6I9XL48_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="21">EGF-containing fibulin-like extracellular matrix protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">EFEMP1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fibulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914701.1">
        <property type="nucleotide sequence ID" value="XM_014059226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XL48"/>
    <dbReference type="GeneID" id="106543258"/>
    <dbReference type="KEGG" id="tsr:106543258"/>
    <dbReference type="CTD" id="2202"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000418">
        <property type="entry name" value="EGF-containing fibulin-like extracellular matrix protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000201">
        <property type="entry name" value="EGF-containing fibulin-like extracellular matrix protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050751">
        <property type="entry name" value="ECM_structural_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055088">
        <property type="entry name" value="Fibulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034:SF102">
        <property type="entry name" value="EGF-CONTAINING FIBULIN-LIKE EXTRACELLULAR MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026872148" evidence="18">
        <location>
            <begin position="18"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="124"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="165"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="205"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="245"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013914701.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="49567" checksum="20A964D9765F3EB9" modified="2020-10-07" version="1">MVIFAVLAALLLATGHSQEADETITFTQCTDGYEWDPIMQRCKDIDECEIVHEACKGGMKCVNHFGGYLCLPRTAQIIVNNGQEQTASDTGSRNGNPIRRTPQVPSQIQCAAGYEANDHNVCQDIDECTTSGHTCRSDQVCVNLRGSFTCQCRTGYQKRGDQCFDIDECTFPSFCHHRCVNSAGSYHCHCNAGFQLTPDNRTCADINECETGNPCAQLCYNIVGSFLCQCNQGFQLSPDRISCDDVDECRASNFLCQYQCVNEPGRYSCACPDGYQVVRGRNCQDINECETRNECREDELCSNYPGGYRCYPRNPCQEPYVLASENRCICPVSNPLCRDLPYSIVHKYMSIHSERTVPSDIFQIQATTIFPNTINTFRIKSGNDNGDFFLRQTSSVSAMLVLVKPLTGPREHIIDLELLTVNNINYRTSSVLRLTLIVGPYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLL5</accession>
    <name>A0A6I9XLL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor AP-2-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">AP-2 transcription factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Activating enhancer-binding protein 2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Activator protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TFAP2A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911728.1">
        <property type="nucleotide sequence ID" value="XM_014056253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLL5"/>
    <dbReference type="GeneID" id="106540939"/>
    <dbReference type="KEGG" id="tsr:106540939"/>
    <dbReference type="CTD" id="7020"/>
    <dbReference type="OrthoDB" id="6252992at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008121">
        <property type="entry name" value="TF_AP2_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01749">
        <property type="entry name" value="AP2ATNSCPFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="16">
        <location>
            <begin position="216"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013911728.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="48357" checksum="6DF680A15B379B3C" modified="2020-10-07" version="1">MSLLAKMGDWQMLVHSFSAMDRHDATSNGTARLPQLGSVGQSPYTSAPPLSHTPNADFQPPYFPPPYQPIYPQSQDPYSHVNDPYSLNPLHAQPQPQHPGWPGQRQNQDTGLLHTHRGLPHQLSGLDPRRDYRRHEDLLHGPHGLGSGLGDIPIHSIPHSLEDVPHVEDPGINIPDQTVIKKGPVSLSKSNANAVSSIPINKDALFGGVVNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAVAEFLNRQHSDPNEQVTRKNMLLATKQICKEFTDLLAQDRSPLGNSRPTPILEPGIQSCLTHFNLISHGFGSPAVCAAVTALQNYLTEALKAMDKMYLSNNPNSHTDNSTKSGDKEEKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNP3</accession>
    <name>A0A6I9XNP3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anion exchange transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC26A7</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917589.1">
        <property type="nucleotide sequence ID" value="XM_014062114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNP3"/>
    <dbReference type="GeneID" id="106545517"/>
    <dbReference type="KEGG" id="tsr:106545517"/>
    <dbReference type="CTD" id="115111"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="6">
        <location>
            <begin position="48"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917589.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17538" checksum="AA3350256B704968" modified="2020-10-07" version="1">MTEAKRKQENAIWRKIHAFRYKNLKLWCVNRLPLLEWAPHYCWKMDLLPDIVSGMMLAVQQVTQGLAFAILSSVHPVFGLYGSFFPAIIYAIFGMGRHVAPGTFALTSLISANAVERLVPPISNFTSDNTSEVLGLSEFEMQRIGVAAAVSLLGGLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNP6</accession>
    <name>A0A6I9XNP6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydroxyacylglutathione hydrolase, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106543929</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915511.1">
        <property type="nucleotide sequence ID" value="XM_014060036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNP6"/>
    <dbReference type="GeneID" id="106543929"/>
    <dbReference type="KEGG" id="tsr:106543929"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF80">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013915511.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="17356" checksum="9A39C3806CDC6407" modified="2020-10-07" version="1">MVCAGGGRIGPALLLLAGPLRKGFSTFQVNFAHPEFERRPPQIISQANMKVEILPALTDNYMYLLVDEETKEAAIVDPVQPQKVLDAAKKHGVKLTTVLTTHHHWDHAGGNEKLAKMEPGLRVYGGDSRVGALTQKVSHLTTFQVKKHQQWWRGGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNQ1</accession>
    <name>A0A6I9XNQ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106543932</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106543932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915516.1">
        <property type="nucleotide sequence ID" value="XM_014060041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNQ1"/>
    <dbReference type="GeneID" id="106543932"/>
    <dbReference type="KEGG" id="tsr:106543932"/>
    <dbReference type="OrthoDB" id="9043293at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915516.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="28004" checksum="2F889C0D247174C0" modified="2020-10-07" version="1">MGRRDLPLLSAKGKTGKACAFCLGITTVLFLFCFVVFLILYTQERHEKITAQEALESKTQEREAMWIQLLSSNQTLMETERLLGMKKKENQDLKSTLATTNQTLGEKMRLLEETEQERSRLQEHLGIVKHNLTTMGGHWNSCQAELYSVQENITSFVAIISKQAAEGQKLKDLIDHLLEQVSSKTRQLADAKRQFQEERNNFDKYRQEVAKPAPENDIPCDKGILRDCNLVFLPFILVELLLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS00</accession>
    <name>A0A6I9XS00_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Collagen alpha-6(VI) chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106546482</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918834.1">
        <property type="nucleotide sequence ID" value="XM_014063359.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546482"/>
    <dbReference type="KEGG" id="tsr:106546482"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000004">
        <property type="entry name" value="collagen alpha-6(VI) chain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF20">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFA" evidence="10">
        <location>
            <begin position="77"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="10">
        <location>
            <begin position="294"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="10">
        <location>
            <begin position="499"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="10">
        <location>
            <begin position="686"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="10">
        <location>
            <begin position="873"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="10">
        <location>
            <begin position="1061"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1459"/>
            <end position="1592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013918834.1"/>
        </source>
    </evidence>
    <sequence length="1602" mass="179035" checksum="CC91A60E38A3C0FD" modified="2020-10-07" version="1">MAQVYQQFPVDGTMVEAQIIVTHRGAFKCPQQALSVLALETPEAVAYERFQELPPSEAAEQTAKDWNIAQNLKMLLILIFIITGTQTFMAQKPVSKPVDLIFLVSSSNTSFPHIKTFISTAVKFLPFDPNEYRIALAQYSDQVYSEFQLDTYKEKNPMLNHIKRKMVLRTGLLKTGSALYRVQEIDYWKPILGEGRSKILIVITSQQSKDDIKNAGWLLQKAGVKIISIGVEEASIDELRLLATKPFYFFFPKIEELSLFAQNISRIVAELYRIQNAMLCFLLIIACHSDGVADLVFIVDAGTSQTSFEKMQLFLENLVSSLDVNQKCIRIGLVTFSTKPQVISSLQMTTDGIHVVQNIQGLSPKPGKANIGTAINFTRQKVFSVSSGSRKAQGVEQIALLISHRPSEEDVRDAAKLLRRAGVTVFAIGIKEANITQLTQIAAYPPPQYVTQLSTFSQLLNKSLDFQKKIMTQIQEKLYMDTKRTELLKRGCVDMEEADIYFLIDGSSNLDYFDFVDLKLFLKEIIRLFAIGPNKVRIGVIQYAETSEVEFDLEEYGKTNDILKAIDNIRQIGGTPPYTGAALTIIQSLFRKLQSQPSRTVPCHLIVLLDQISKDNVEEPARRLRNEKINLYAIGVRHTNASQIYTIADSNNRAYFVNDFASLKYIKNDVVREICATEVCKEKKTDIVFLVDSSRSSATEDFEKMKNFMRLLVDKSDIGPETVHIGVVQFSDKCQEEFQLNQYFTKPDINNAIGRMSLMGQSTFTGEALQFVSNYFKPAKGSRLYVKKVLILITNGEAQDEVKNHAEALREENIIIYSVGLFQANKMQLMEISGKPEMVYYVEDFEVLKYLKNEILFEICSSSDECRRIERLDIIFVIDGSGSIDPKEYDIMKDFMITLVKKSDVGYDRVQFGAVKYSAEPETFFYLNQFNTKSAIIEAIQNDKPIGETTYTAKALRHSESLFSKKHGSRKHIPQVIIVITDGDSHDAAELEEVSKNLRARGIVIYAIGIERAKPDELLTMAGSEDRYFYVNTFEGLKNLYPRLSDNICGVSKSECGIPADLVFLIDGSNSISDSDFTKMKIFLQDVIHPFDTTHNVQVGIAQYSDRYQEEFSLNIFSRKSELENQIRHIQQMEGLQTYIGAALKKVKLYFTPEGGSRINEKIQQILLVITDGRSHDRVVQAAEDLRKKGVDIYAIGVGRIDHLQLSQIAGSSDRKYTVDNFSELKVIKKRLVDDICEQEDKTSCFVDIVVGIEVSSQNQGDHVFLGQPQLEFYLPQITSALTSLTSLSCNAGSQTQTSVALKIKNTDPLVASKFQIDSEKLINSLVGTTITNASHLTAEYLDSLWETFQIKSANRRKVLLIFTDGLDDRIEILKDKSEELKKKGLDALITVVLEGASNFDDLQFIEFGKGYGYKIQLNIGMRDIASQLFKYMNNIAERTCCCIYCKCIGEEGEPGEQGKYGMEGSQGFEGSPGHLGDEGEPGPRGLPGSDGDKGYSGCQGNRGPKGQRGIIGEKGSNGEKGFDGINGELGSPGLPGFKGEKGDPGNQGSPGVKGIHGECGKNGFQGDLGERGPFGPTGDPGSKGTKVRTKERKIYIDFSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSY9</accession>
    <name>A0A6I9XSY9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Copine-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Copine V</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106542532</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable calcium-dependent phospholipid-binding protein that may play a role in calcium-mediated intracellular processes. Plays a role in dendrite formation by melanocytes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the copine family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913788.1">
        <property type="nucleotide sequence ID" value="XM_014058313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSY9"/>
    <dbReference type="GeneID" id="106542532"/>
    <dbReference type="KEGG" id="tsr:106542532"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000117">
        <property type="entry name" value="copine-5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000013">
        <property type="entry name" value="copine-9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF51">
        <property type="entry name" value="COPINE-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="8"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="166"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="333"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013913788.1"/>
        </source>
    </evidence>
    <sequence length="588" mass="65468" checksum="170C97B0CC465435" modified="2020-10-07" version="1">MEEDVLDKLEDMASVQDFDPLTGSIPATKVELTVSCRNLLDKDTFSKSDPLCVLYTQGIETKQWREFGRTEVIDNTLNPDFVRKFILDYFFEEKQNLRFDLYDVDSKSPDLSKHDFLGQTFCTLGEIVGSPGSRLEKPLMMGAFTLHSKTGKPMPSVSNGGIPGKKCGTIILSAEELGNCRDVATMQFCANKLDKKDFFGKSDPFMVFFRSNEDGTFTICHKTEVVKNTLNPVWQSFAIPVRALCNGDYDRTIKVEVYDWDRDGSHDFIGEFTTSYRELARGQSQFNVYEVVNPKKKMKKKKYSNSGTVTLLSFATESEHTFLDYIKGGTQINFTVAIDFTASNGNPSQSTSLHYMNPYQLNAYAMALKAVGEIIQDYDSDKMFPALGFGAKIPPDGRVSHEFPLNGNCDNPHCSGIEGILEAYHQSLKTVQLYGPTNFAPVVNHVAGYASAVQDGSQYFVLLIITDGVISDMAQTKEAIVNAAKLPMSIIIVGVGQAEFDAMVELDGDDVRITSRGRTAERDIVQFVPFRDYIDRTGNHVLSMARLAKDVLAEIPDQFISYMKGRGIKPNPAPPPYSPSSHTLQTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT12</accession>
    <name>A0A6I9XT12_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor YJU2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CCDC94</name>
        <name evidence="8" type="synonym">YJU2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates. May protect cells from TP53-dependent apoptosis upon dsDNA break damage through association with PRP19-CD5L complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the spliceosome. Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CWC16 family. YJU2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913808.1">
        <property type="nucleotide sequence ID" value="XM_014058333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT12"/>
    <dbReference type="GeneID" id="106542554"/>
    <dbReference type="KEGG" id="tsr:106542554"/>
    <dbReference type="CTD" id="55702"/>
    <dbReference type="OrthoDB" id="674963at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03226">
        <property type="entry name" value="YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043701">
        <property type="entry name" value="Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF1">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="180"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913808.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="37973" checksum="64BD2A6FB3FD2182" modified="2020-10-07" version="1">MSERKVLNKYYPPDFDPSKIPKLKLPKDRQYVVRLMAPFNMRCKTCGEYIYKGKKFNARKETVQNESYLGLPIFRFYIKCTRCLAEITFKTDPENTDYAMEHGATRNFQAEKLLEEEEKRLQKEREDEELNNPMKVLENRTKDSKLEMEVLENLQELKDLNQRQANVDFESMLHQYKEFEEQQKQRELEEDEQEMKSMLEQAQSRRLLEDSDSGEDLPPARPKPLAKGKPTDILKQDSEPQAKKPKLESWERSVGKLSVKTQLSGLVTTKKKPISRPANGPGRQEAASEPGGREQHSLGPSTATMHSSNTGVFSLGLLGAYSDSDDSKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTP2</accession>
    <name>A0A6I9XTP2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Poly(RC)-binding protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12" type="primary">PCBP4</name>
    </gene>
    <organism evidence="8 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919435.1">
        <property type="nucleotide sequence ID" value="XM_014063960.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919443.1">
        <property type="nucleotide sequence ID" value="XM_014063968.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919451.1">
        <property type="nucleotide sequence ID" value="XM_014063976.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919459.1">
        <property type="nucleotide sequence ID" value="XM_014063984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTP2"/>
    <dbReference type="GeneID" id="106546950"/>
    <dbReference type="KEGG" id="tsr:106546950"/>
    <dbReference type="CTD" id="57060"/>
    <dbReference type="OrthoDB" id="442947at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22520">
        <property type="entry name" value="KH-I_PCBP4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22523">
        <property type="entry name" value="KH-I_PCBP4_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000002">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000005">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="26"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="110"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="254"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013919435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013919451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919459.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="34382" checksum="7180A9EC531A0BFC" modified="2020-10-07" version="1">MHSYSVASPDRMSGSDNGLDETELSITLTLRMLMHGKEVGSIIGKKGETVKKIREQSTARITISEGSCPERITTITGSTDAVFRAVSMIAFKLEEDLGNGATNGGTISKPPVTLRLVIPASQCGSLIGKAGTKIKEIRETTGAQVQVAGDLLPNSTERAVTVSGVPDAIIQCVRQICAVILESPPKGATIPYHPSLSLGPVLLSANQGFSMQGQYSGISQAEVTKLQQLSGHPVSFSSLAQTPSLVAGLDANSQNSSQEFLVPNDLIGCIIGRQGSKISEIRQMSGAHIKIGNQTEGSSERHVTITGTPVSITLAQYLITACWTPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XU67</accession>
    <name>A0A6I9XU67_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Complement C1r subcomponent-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106542899</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914233.1">
        <property type="nucleotide sequence ID" value="XM_014058758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XU67"/>
    <dbReference type="GeneID" id="106542899"/>
    <dbReference type="KEGG" id="tsr:106542899"/>
    <dbReference type="OrthoDB" id="9985152at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001867">
        <property type="term" value="P:complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035821">
        <property type="term" value="P:modulation of process of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000062">
        <property type="entry name" value="mannan-binding lectin serine protease 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255">
        <property type="entry name" value="COMPLEMENT COMPONENT 1, S SUBCOMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255:SF13">
        <property type="entry name" value="MANNAN-BINDING LECTIN SERINE PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="47"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013914233.1"/>
        </source>
    </evidence>
    <sequence length="326" mass="36102" checksum="DB111C4C1C23991B" modified="2020-10-07" version="1">MVPNVTGIFTCDTKGAWRSSELGTHLPSCQPVCGQPSRALPTLVKRIIGGRNAEPGFFPWQALIVVEDTSRVPNERWFGSGALLSESWVLTAAHVLRSQRRDNTLIAVSKEHVNVYLGLHDVRNKIDAVNRTVEKIILHESFDIQNYNHDIALVKLKEKVAMGPYIMPVCLPQDPHELGGPQANTLGLVAGWGISNPNVTVDEVISSGMQTLSDVLQYVKLPVVLHAECKTSYESRSGNYSVTENMFCAGYYEGGKDTCLGDSGGAFVIRDLDNRRWVAQGLVSWGGPEECGSKQVYGVYTKVSNYVDWLQEKTEWTFLNSSLEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUL0</accession>
    <name>A0A6I9XUL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Iodothyronine deiodinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DIO2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>3'-iodo-L-thyronine + iodide + A + H(+) = 3',5'-diiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:82899"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:195762"/>
            <dbReference type="ChEBI" id="CHEBI:232695"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:82901"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3,3'-diiodo-L-thyronine + iodide + A + H(+) = 3,3',5'-triiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:82575"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57261"/>
            <dbReference type="ChEBI" id="CHEBI:176514"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:82577"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the iodothyronine deiodinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919774.1">
        <property type="nucleotide sequence ID" value="XM_014064299.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547214"/>
    <dbReference type="KEGG" id="tsr:106547214"/>
    <dbReference type="CTD" id="1734"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004800">
        <property type="term" value="F:thyroxine 5'-deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042403">
        <property type="term" value="P:thyroid hormone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000194">
        <property type="entry name" value="Iodothyronine deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000643">
        <property type="entry name" value="Iodothyronine_deiodinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008261">
        <property type="entry name" value="Iodothyronine_deiodinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781">
        <property type="entry name" value="IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781:SF20">
        <property type="entry name" value="TYPE II IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00837">
        <property type="entry name" value="T4_deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001330">
        <property type="entry name" value="IOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01205">
        <property type="entry name" value="T4_DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0711">Selenium</keyword>
    <keyword evidence="6 13 15" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3 13" id="KW-0893">Thyroid hormones biosynthesis</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="active site" evidence="12">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="15">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="15">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013919774.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="31433" checksum="D3CB1A4CF73D2591" modified="2020-10-07" version="1">MGLLSIDLLITLQILPVFFSNCLFLALYDSVVLLKHMVLLLSCSKAARGEWRRMLTSEGLRCVWNSFLLDAYKQVKLGGDAPNSNVIHIPNGTAESSSNCRWKSSMGKYGSECHLLDFAKSDRPLVINFGSATUPPFTNQLSAFGKLVEEFSSVADFLLVYIDEAHPSDGWAAPGISSSFEVKKHRNQEDRCAAAHQLLKKFSLPPQCQIVADCMDNNANVAYGVSFERVCIIQRQKIAYLGGKGPFFYNLQEVRLWLEQNFRKRUNPNVPDKNSRGLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUV1</accession>
    <name>A0A6I9XUV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106538485</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908455.1">
        <property type="nucleotide sequence ID" value="XM_014052980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUV1"/>
    <dbReference type="GeneID" id="106538485"/>
    <dbReference type="KEGG" id="tsr:106538485"/>
    <dbReference type="OrthoDB" id="6692864at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008397">
        <property type="term" value="F:sterol 12-alpha-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24306">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24306:SF0">
        <property type="entry name" value="7-ALPHA-HYDROXYCHOLEST-4-EN-3-ONE 12-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908455.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="65530" checksum="3F7C2B3FE252F8BA" modified="2020-10-07" version="1">MLLTTSVTGKPQRLDYLTDGIRQSHAELPYDWMVYKCQFPRAPDQIYPRTPVMKLWETVVCALLSTFIALIFGGLYRIGVFRRKRPKEPPLDKGLIPWLGHGLSFGKKPALLLERMKKKHGDIFTMLVAGRYIHVLMDPHMYGFIFKKSRSKLDFGVFAANVVSNVFNFHPTTTHHKIGEDGSKKYLRGKNLLALNQALMQNLKEILLESAEEEKWRQEGLWHFSYKSIFQAAFLCLFGAMPELEDNSNDKERRCKPYEKLFKDFQQFDEFFPQMIFSSMDSKTKKEIQRLKNYFWDLLSVEKIDKREDVSIWLADQDQQLADCGMNEKMRTQVQLLFLWASQANTGPVTFWLLAYLLKHPEAMKAIRAEIDQVLKETGQEDNPVDFSLQAFKTPLLDSAVEEVLRLKAGSFVFRSVMEDFNLQMDDGKEYALRKGDHLLLFPFMGLHMDPEIYPEPKTFKYDRFMSPEGKKKEFFKNGKKLRTCVMPFGGGSSMCPGRFFAVSEMKILTILMLTFFDMELVNPEEELPSSIEHRIVIATAHPTRDIDFKFRRRLQSSKPGVQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW59</accession>
    <name>A0A6I9XW59_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Large neutral amino acids transporter small subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC7A5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918377.1">
        <property type="nucleotide sequence ID" value="XM_014062902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW59"/>
    <dbReference type="GeneID" id="106546121"/>
    <dbReference type="KEGG" id="tsr:106546121"/>
    <dbReference type="CTD" id="8140"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000056">
        <property type="entry name" value="Y+L amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF315">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013918377.1"/>
        </source>
    </evidence>
    <sequence length="448" mass="49002" checksum="D2AF1FF7EE569A8C" modified="2020-10-07" version="1">GPAEDDGAAAAGGGVALKRNITLLNGVAIIVGTIIGSGIFVSPTGVLKEAGSPGLALVVWAACGAFSIAGALCYAELGTTISKSGGDYAYMLEVYGSLPAFLKLWIELLIIRPSSQYIVAFVFATYLLKPLFPTCYVPLGATKLIACLCVLLLTAVNCYSVKAATRVQDAFAAAKLLALALIITLGFVQLGKGDVMNLKPESSFEGTKTGVGNIVLALYSGLFAYGGWNYLNFVTEEMINPYRNLPLAIIISLPIVTLVYVLTNLAYFTTLSVNEMLVSEAVAVVTFSERKTSLRWVISTVGQPFCPHVCRVSELRMKQALCLMTLFYAFSNDIFSVINFFSFFNWLCVALAIIGMMWLRYKKPELERPIKVNILLPIFFILACNFLIVVSFWMTPKECAIGFAIIFSGVPVYMIGVWWRNKPKWLLQSIYSTTILFQKLMEVVPQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW61</accession>
    <name>A0A6I9XW61_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="10">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106547715</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the FMO family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013920429.1">
        <property type="nucleotide sequence ID" value="XM_014064954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW61"/>
    <dbReference type="GeneID" id="106547715"/>
    <dbReference type="KEGG" id="tsr:106547715"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000138">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013920429.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="49234" checksum="65631BB92CB81C02" modified="2020-10-07" version="1">MVPTKLRVAVIGAGAAGLCAARHILASSEFFAPPVVFESSDRLGGTWIYTEEKEDATGRIIHSSMYRDLRTNLPKESMAFPDFPFDPSLPSFLHHSDVLAYLDSYAEQSGVCEHIRFQWQVEEVRPVPRDAGSLGGWEITARVQHPESAQQVTENFDAIMVCTGHYTVPFIPPIPGLDSFQGHLLHSHSYRYPEPFANQSVVLVGAGPSGVDLALQLSNVAAQVVLSHNRQHVCGLPKEVIQVPPLLKVGQKTVVFTDGSKMPADVLILCTGYKYSFPFLDLAQLGLQETDYGVGPLYQHLLPPQHPSLFLIGVCQQICPFPHFYCQVLFALAVLSGRCSLPSVTDMEAEVERQLGLHLKKGGLARYFLKMKEQQWSYMEDLAHLAGFPPVPPAVQQIYEDTHASRLCNVSTYRSRNYRLLGPSAWELVQDWGENCMKTEDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXF6</accession>
    <name>A0A6I9XXF6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Membrane-spanning 4-domains subfamily A member 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106546210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918491.1">
        <property type="nucleotide sequence ID" value="XM_014063016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXF6"/>
    <dbReference type="GeneID" id="106546210"/>
    <dbReference type="KEGG" id="tsr:106546210"/>
    <dbReference type="OrthoDB" id="10071849at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF155">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918491.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="26986" checksum="CB4A179071234F82" modified="2020-10-07" version="1">MASGTIIFIPSNGANIIQTAPGLPSVALQASGAIPYPQYGVQQLGISTSAPQQVPQKSPLERFLKAEPKVLGAVQILIGLIHIGFGAVSLCLFPPYYLPLSGFGGYPFWGGIFFISSGSLCVAAANRPNHTLVKSSAGLNITSAIMALIGVILYLCELRLSNNFWRYEDDYDTYNLISLQSVGHGLSSVLLLFSVLEFCIAVSLAHFGCQAACCTDDQPTVLFVPYQVMGGGEVASEPNPPPPPTYDNVITKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXF8</accession>
    <name>A0A6I9XXF8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rho guanine nucleotide exchange factor 10-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106543741</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915293.1">
        <property type="nucleotide sequence ID" value="XM_014059818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXF8"/>
    <dbReference type="GeneID" id="106543741"/>
    <dbReference type="KEGG" id="tsr:106543741"/>
    <dbReference type="OrthoDB" id="28697at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039919">
        <property type="entry name" value="ARHGEF10/ARHGEF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877:SF14">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915293.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="31826" checksum="AB05EA961FBBBC1B" modified="2020-10-07" version="1">MIAKGIYQNLNQSVAHDWSLGLQRLILKKEDEIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTFTPAIKQSWIRSLQMAKLALADENLLGWFCIDDDGNQIKKEKHPLLVKHMPVMMAKQQEFKVECAAYNPEPCLCEESDPDSLSTEYGFLWIGSCTNQMGQIAIVSFQNSSPKLIECFNVESRILCMVYIPEDEKSKESNNMFPESGDMATKPLNVPTICVGMEEGSISLYKSSQGSKKIRLQHFFAPEKSTVMSLAYKTHHLFAGLVNGNIAIYTKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXZ8</accession>
    <name>A0A6I9XXZ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dentin sialophosphoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">DSPP</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013919052.1">
        <property type="nucleotide sequence ID" value="XM_014063577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXZ8"/>
    <dbReference type="GeneID" id="106546658"/>
    <dbReference type="KEGG" id="tsr:106546658"/>
    <dbReference type="CTD" id="1834"/>
    <dbReference type="OrthoDB" id="9050092at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027000892" evidence="2">
        <location>
            <begin position="17"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="615"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="686"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="730"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919052.1"/>
        </source>
    </evidence>
    <sequence length="760" mass="81039" checksum="0815B8DC61009BDB" modified="2020-10-07" version="1">MKKTIVFLCIVVTAWALPLLQHEQRNIISTVENVDTSNADHHDNGGEDLRVLLNNVGYTNGNINCVYGRSIGHGHEECKTEESITNADRVNGTAGFVNVSENEGGRPEDNLRINENREYIPAEANATERKEVTLEGEYFSDSNYGIAEENINYTAPGELFESDPNDKQHGTEDQINDQTGEEFRDSRGLRGNHIQSFIDMDEDRYAIYGRGDNTENILMGSEARGESIHWAYIDEGGSGNGGDPNEEGSSDGWGDGTSESTTNHGENETETHNDRNNTQACDTKITNPNKEGEGDADTVEDSLNTDNEEGFQGRGDVSPETKHVTCLEKKRSDGLDSNGNTKDTENNTEAVNNSTTNGANMHDKVMNIPNRETAHQDTIQTPQRSDAEGSSGHEDTSENESNSVYSFSDELMQGDEPNQSQDSEGSENSSQQDLPNQASDGESTSLGEEEEQSSNAGSEQSDSKGQDNESQQESNESSRHPGGDDSDNSGPSVSSESESQPDSSQSQSESNSENDSENESISESDSKTESSIDSGSESKSSSESDSESESSSESDSESKSSSESDSESKSSSESDNESDSSSESDSESDSKSDSSSESDSSSSSESDSKSKSSSESDSSSSSESDSDSSSSSSSENDSSSSDSESSSNESESSSSESDHKSDSSSESSSSSENESETSSENEDNNQADSSESDSQSNSDNESDSANQSSSDSAADTTEDSNETESEASEDSNSSSHSNSAASDSDSLSQGSKSNDLTSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY19</accession>
    <name>A0A6I9XY19_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Adhesion G protein-coupled receptor A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ADGRA1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915498.1">
        <property type="nucleotide sequence ID" value="XM_014060023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY19"/>
    <dbReference type="GeneID" id="106543917"/>
    <dbReference type="KEGG" id="tsr:106543917"/>
    <dbReference type="CTD" id="84435"/>
    <dbReference type="OrthoDB" id="10031018at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051963">
        <property type="entry name" value="Adhesion_GPCR_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45930:SF3">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45930">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 124-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
        <location>
            <begin position="17"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="458"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="466"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013915498.1"/>
        </source>
    </evidence>
    <sequence length="571" mass="64074" checksum="391CFAF1CF4F7B64" modified="2020-10-07" version="1">MDQKTVWPLSQYPGEFLHPVVYACTAVMLLCLFASIITYIVHHGTIRISRKGWHMLLNFCFHSALTFAVFAGGINRVKYPVICQAVGIVLHYSTLSTMLWIGVTARNIYKQVTKKPQTAQNSDQPSYPKQPLLRFYLIGGGVPFIICGITAAINLNNYGIKSYAGAQFCWMSWEPSLGAFYGPLAFILLVTCVYFLCTFVQLRRHPERKYELKERIEDQQRLASPELGHSHMTDIGSVSQATCSMMSSSLLENEHSFKAQLRAAAFTLFLFTATWTFGALAVSQGHFLDMIFSCLYGAFCVTLGLFILIHHCAKRDDVWHCWWSCCPSRRNTYSVQLNVRPKVNINGESQAHASCLQESPCPSKSALFNHPAVSHCKLTNLQAVQNHVNCLSPVTPCCAKMHCDQLLEDEAHIHVHNEGTFRPNMHIHRCLKSRTKPRYFSRHRSAGEREYAYHIPSSIDGSIHSSHTDSPHSTHDSHSGHRRACCAKNSPYPTITQPESSDSSTVIYSCAKVPETETGPHFEMHPRTQSLPFSTTSHNGILKGNLHEAMIYSSDSTGNIKTGPWKNETTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY25</accession>
    <name>A0A6I9XY25_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Heat shock factor protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HSF4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921134.1">
        <property type="nucleotide sequence ID" value="XM_014065659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY25"/>
    <dbReference type="GeneID" id="106548312"/>
    <dbReference type="KEGG" id="tsr:106548312"/>
    <dbReference type="CTD" id="3299"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF213">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="9">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921134.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="14037" checksum="FD4553EFA770A633" modified="2020-10-07" version="1">MQDSTTALAMDGYTSNVPAFLTKLWTLVEDPETNHLICWNTNGTSFHVFDQSQFAKEVLPKYFKHNNMASFVRQLNMYGFRKVVNIEQGGLVKPERDDTEFQHLYFLQGHEHLLELIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYR2</accession>
    <name>A0A6I9XYR2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein SSXT isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SS18</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SS18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915718.1">
        <property type="nucleotide sequence ID" value="XM_014060243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYR2"/>
    <dbReference type="GeneID" id="106544090"/>
    <dbReference type="KEGG" id="tsr:106544090"/>
    <dbReference type="CTD" id="6760"/>
    <dbReference type="OrthoDB" id="10265171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007726">
        <property type="entry name" value="SS18_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107:SF2">
        <property type="entry name" value="PROTEIN SSXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107">
        <property type="entry name" value="SYNOVIAL SARCOMA ASSOCIATED SS18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05030">
        <property type="entry name" value="SSXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SS18 N-terminal" evidence="9">
        <location>
            <begin position="14"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="326"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013915718.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="45522" checksum="82344726F1759751" modified="2020-10-07" version="1">MSVAFAAPRQRGKGEITPAAIQKMLDENNHLIQCIMDYQNKGKTLECSQYQQLLHTNLVYLATIADSNQNMQSILPPPPTQNMPMGPGGMNQSGPPPPPRTHNMSSDGMVGGGPSAPHMQNQMNGQMPGPNHMPIQGPGPSQLSMTNNSMSIPSNNHGSMGAYNHSVPSSQSIPGQSQMTISQGQPMTSYGPRPNININQGPMMHQQPPSQQYNIPQGGAQHYQGQQPPMGMISQVNQGNHMMGQRQIPPYRPPQQGPPQQYSGQEDYYGDQYSHGGQGPPEGMNQQYYPDGHSDYGYQQPSYPEQGYDRPYEDSSQHYYEGGNAQYGQQQDAYQAPPQQPGYQQQQYPGQQGYPGQQQGYGPSQSAPGPQYSNYPQGQGQQYGGYRPTQPGPPQPPQQRPYGYDQGQYGNYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0R4</accession>
    <name>A0A6I9Y0R4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Serine/threonine-protein kinase BRSK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">BRSK1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920432.1">
        <property type="nucleotide sequence ID" value="XM_014064957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0R4"/>
    <dbReference type="GeneID" id="106547719"/>
    <dbReference type="KEGG" id="tsr:106547719"/>
    <dbReference type="CTD" id="84446"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14340">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048622">
        <property type="entry name" value="BRSK1_2-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF36">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BRSK1 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21122">
        <property type="entry name" value="KA1_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21115">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="28"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="311"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="518"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013920432.1"/>
        </source>
    </evidence>
    <sequence length="577" mass="65531" checksum="29E08C7AC3E1FD7D" modified="2020-10-07" version="1">MSSGKEAGGVHPYQSQQSQQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSIWSLPLISLPKWDRVNNSYHFSEFLKMIISYYMSGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGIFHMPHFIPPDCQNLLRGMIEVEPEKRLSLEQIQKHPWFFLDPDVLDSMHSLGCFRDKNKLKQELQNEGENQEKMIYYLLLDRKERYPSCEDEDLPPRNDVESPSSTPCGTPPSSPGVAGVAWKTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSSLTPESSPELAKKSWFGNFINLDKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKSSGGPSVFQKPVKFQVDISYSEGGEAHKDSGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGQQLRFPGTPTRSCQPSRRSESDIPTEQHRHGSKDKKLVSSNGTQVQQPQTPSPQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1N0</accession>
    <name>A0A6I9Y1N0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NEDD8-conjugating enzyme UBC12</fullName>
            <ecNumber evidence="7">2.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">NEDD8 carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">NEDD8-conjugating enzyme Ubc12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106548057</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.34" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013920837.1">
        <property type="nucleotide sequence ID" value="XM_014065362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1N0"/>
    <dbReference type="GeneID" id="106548057"/>
    <dbReference type="KEGG" id="tsr:106548057"/>
    <dbReference type="OrthoDB" id="10249039at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061654">
        <property type="term" value="F:NEDD8 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000239">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="15">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013920837.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="13902" checksum="41EBBC981A698A41" modified="2020-10-07" version="1">MLALQGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSIYFERCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y333</accession>
    <name>A0A6I9Y333_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="13">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PDIA3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013917608.1">
        <property type="nucleotide sequence ID" value="XM_014062133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y333"/>
    <dbReference type="GeneID" id="106545536"/>
    <dbReference type="KEGG" id="tsr:106545536"/>
    <dbReference type="CTD" id="2923"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03073">
        <property type="entry name" value="PDI_b'_ERp72_ERp57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000045">
        <property type="entry name" value="Disulfide-isomerase A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000054">
        <property type="entry name" value="Disulfide-isomerase A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000077">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000017">
        <property type="entry name" value="Protein disulfide-isomerase A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027139884" description="Protein disulfide-isomerase" evidence="13">
        <location>
            <begin position="20"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="334"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="49"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="397"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013917608.1"/>
        </source>
    </evidence>
    <sequence length="498" mass="56019" checksum="3EB59104167D6D25" modified="2020-10-07" version="1">MATLLLALLLAAAAWPARASDVLELSDDDFDSGLADRNVALVEFYAPWCGHCKRLAPEYEAAATRLKSIVPLVKVDCTANSETCNKYGVSGYPTLKIFRNGEESGTYDGPRTADGIVSHLKKQAGPASVPLSPEGFEKFINEKDAAVVGFFRELFGDAHSEYMKAASNLRDHYRFGHVSDEDLIKKYEPDGEGIVLFRPQHLANKFEDSSVRYTEEKITTGKIKKFLQENIFGLCPHMTEDNKELIQGKDLLVAYYDIDYEKNPKGSNYWRNRVMKVAQSFLDAGHKLNFAVASRKTFGHELTEFGLDGSTSDIPLVAIRTAKGEKFAMQEEFSRDGKALERFLQDYFDGKLKRYLKSELIPENNDGPVKVVVAENFDEIVNAESKDVLIEFYAPWCGHCKNLEPKYKELGEKLNKDPHIVIAKMDATANDVPLPYEVKGFPTIYFAPAGSKQSPKKYEGGREVSDFVSYLKREATYPPILQEDEKPKKKKKVPKEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y348</accession>
    <name>A0A6I9Y348_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">B-cell CLL/lymphoma 9-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BCL9L</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BCL9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911315.1">
        <property type="nucleotide sequence ID" value="XM_014055840.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540650"/>
    <dbReference type="KEGG" id="tsr:106540650"/>
    <dbReference type="CTD" id="283149"/>
    <dbReference type="OrthoDB" id="7668649at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015668">
        <property type="entry name" value="Bcl-9/Bcl-9l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024670">
        <property type="entry name" value="BCL9_beta-catenin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15185:SF3">
        <property type="entry name" value="B-CELL CLL_LYMPHOMA 9-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15185">
        <property type="entry name" value="BCL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11502">
        <property type="entry name" value="BCL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B-cell lymphoma 9 beta-catenin binding" evidence="5">
        <location>
            <begin position="418"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="7"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="124"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="446"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="887"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1142"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="356"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="915"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="938"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1016"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1039"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1195"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1221"/>
            <end position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911315.1"/>
        </source>
    </evidence>
    <sequence length="1454" mass="150433" checksum="601C9019EAC46F64" modified="2020-10-07" version="1">MIVFVPLSLPLPTQEAFGPTGSASGGGPRDGGLPPPVSLPETSASQMHPDSKLASHGKTGNSSSAHSQPRPHPVSPPPIGHLGAKDVGGAGSQGSRVGQPATGPLALKASQAPVSVFGALKGKVRRERSVSMDSGERREASATPLDKELKGETVPRSKRRCVLERKQPYSGDEWCSGPESEDDDKSLGSSHNCHVAESAMSGSAQLGPGSNPLPALSESNTPGAPHSTAPSLRSDAGGKQGLQLVYVFTTHLANTAAEAVLQGQADSILGYHQQHVPRAKLDQPAPLPKMPGAVEPLPLHLSPANTPQGPPALPPSSQPPGQQAGGPPLGLGQGPLSSSNALPPEGAPEESCQDLTPNSMGNGSNSSSSAAPSSTHPSTPTTLGSLQASTAEGLLGEGASTGPPAAGNGPRNPLNTEGLSKEQLEHRERSLQTLRDIERLLLRGSEAEPFLKGGPGPGEGTILPTQAAAPQPPQAALSGAGVKKYEEPLQSMISQTQSLGGPGLEQEGPLPPGVDLSQPMNMVLQRLGQDSLTPEQVAWRKLQEEYYEEKRRKEEQAGLHGGRPLQEVMLAQPLGGVMLRGPPPPYHSRPGEQWLLRGASLDIQEQLRPTFSGPRGFPGGHVQRGFATMPLESLGPGGGGPRPIQPGLPWGEDLPLRGPGHFAPGGLSYPPVQGEAERFLPSRAREDLLRHSLLEKRPGSLGLEMERVLPTHRQVEPGLFAGEGLGLEFGGARALMSPPPPLQEVEPSALATPTNLNMNVNMNMNLSVQMTPQQQQQQQQQMLLAQKARGAELMSHEEMARGRAPNGTGALMGGPPKMLMGSPFPGQGQQQGFSGQGPYPPVLQEMGGTPDMFGPEQGGMAGTPRLSHVPLPPSTSASASNMHAAPIRGLGRRPSDLALNMGPMNSPSVGRLKSPTLSQAHSPLGTSPSANLKSPQTPSALASLPAASSAPLKSPQVLGSVLNVRSPTSSPGHLKSPSMAVSSPGWAPSPKASLASPGKPALGMGNSAPLGHLEQGAVPSGSRSSSSAPPGNVSGPLNPSLPFPSSPDPPPSQNPLSLMMSQMSKYAMPSSTPLYHNAIKTIATSDDELLPDRPLLPSGAMSGMGGNQPNPMHVNSVGPAPVPSPLGLNLAGQQLLSQEVAAPPMSSPSPLGTGLPMHSGAPVQNSMMPPGPPDALGQPCGPSGQLVFAPRLQQPPANGGIPMAPGGAAGPGLQQPPPPQQHHYPPGLGVPPEELPPQQVPPQQRLAGRLPEPYPSVASVLSDPELSDVIRPTPTGIPEFDLSRIIPSEKPSSTLQYFPKGGEGQGVKGLPPSNLHLASLQNMMVEQGQARPGPQTMPRGMTLCHPGQMPVLGRTGLPPQQSLVGNSLPQGLMLPQQSLLAQQNFLLMQAKQRSISVSGEMYGQPAGHLISPQGPLMGPPSQQNLMVSMRQRSVSLDGQMSYGPGAGSMANLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3J4</accession>
    <name>A0A6I9Y3J4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Intercellular adhesion molecule 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106545819</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. ICAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917953.1">
        <property type="nucleotide sequence ID" value="XM_014062478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3J4"/>
    <dbReference type="GeneID" id="106545819"/>
    <dbReference type="KEGG" id="tsr:106545819"/>
    <dbReference type="OrthoDB" id="6250964at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000194">
        <property type="entry name" value="Intercellular adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000338">
        <property type="entry name" value="intercellular adhesion molecule 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048679">
        <property type="entry name" value="ICAM1_3_5_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013768">
        <property type="entry name" value="ICAM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047012">
        <property type="entry name" value="ICAM_VCAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003987">
        <property type="entry name" value="ICAM_VCAM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13771">
        <property type="entry name" value="INTERCELLULAR ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13771:SF9">
        <property type="entry name" value="INTERCELLULAR ADHESION MOLECULE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21146">
        <property type="entry name" value="ICAM1_3_5_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03921">
        <property type="entry name" value="ICAM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01472">
        <property type="entry name" value="ICAMVCAM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026696278" evidence="12">
        <location>
            <begin position="25"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Intercellular adhesion molecule N-terminal" evidence="13">
        <location>
            <begin position="27"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Intercellular adhesion molecule 1/3/5 D2" evidence="14">
        <location>
            <begin position="225"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013917953.1"/>
        </source>
    </evidence>
    <sequence length="405" mass="44716" checksum="BF3BD365C719D6D0" modified="2020-10-07" version="1">MKLQWFWHFLPCCFLLPVLMPVEAEQHVFDVTIWPENPVVEHGGSLWLNCSTSCQEADARGGLETSLMKEKKDNGSHWAVFQFVNITEWASATECSFSCYGEHKSVQANIMVYQIPEHVMLDPVPIMEVGKAYNLTCRVANAAPIQLLTVTVYKGTEKLHVETFENHTAPEARDIRVTFPITAQQDNHMEEVTCHVALDLRPRGQLLEKTSFSEVLKVFDFRADPHLHTLRYVPASANLSVTCEIAGVFPAEEAQFDLKFAEQRLNASITLSGNTVRAQAQVSSLATGNYQLNCTVSLGPVTKSAVETVHIYSLPQPSLEIHPSEALANNPVTVLCNSSAPEPPSFSLQIQNASGGILASGDEFPLQLNLTAQEEDNGQEFVCVVELEIDGVTMTKHTSATLTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3T4</accession>
    <name>A0A6I9Y3T4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein NDRG1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911800.1">
        <property type="nucleotide sequence ID" value="XM_014056325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3T4"/>
    <dbReference type="GeneID" id="106541006"/>
    <dbReference type="KEGG" id="tsr:106541006"/>
    <dbReference type="OrthoDB" id="741027at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000006">
        <property type="entry name" value="NDRG family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911800.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="35011" checksum="28B465476764FC21" modified="2020-10-07" version="1">MSSDGPDSRVAEQKPLVDKEEAITRLLPDIDVDIQEQDIETVHGSVHVTMCGTPRGNRPAILTYHDIGLNHKTCFNPLFNNEDMQEITQHFAVCHVDAPGQQDGAPSFPPGYMYPSMDQLSETLPGVLKQFGLKSVIGMGTGAGAYILTRFALNYPDMVEGLVLINVNPCAEGWMDWAATKISGWAHALPDMVISHLFGKEEIHSSHDLIHTYRQHIINDMNQSNLHLFVNSYNSRRDLDIERPVPGVNVITLQCPSLLVVGDSSPAVEAVVECNSRLDPINTTLLKMADCGGLPQVVQPGKLTEAFKYFVQGMGYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3X4</accession>
    <name>A0A6I9Y3X4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HIPK4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013911910.1">
        <property type="nucleotide sequence ID" value="XM_014056435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3X4"/>
    <dbReference type="GeneID" id="106541099"/>
    <dbReference type="KEGG" id="tsr:106541099"/>
    <dbReference type="CTD" id="147746"/>
    <dbReference type="OrthoDB" id="437530at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000029">
        <property type="entry name" value="Homeodomain-interacting protein kinase 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF46">
        <property type="entry name" value="HOMEODOMAIN-INTERACTING PROTEIN KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="11"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="457"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911910.1"/>
        </source>
    </evidence>
    <sequence length="603" mass="69498" checksum="4C2DDA0251484D10" modified="2020-10-07" version="1">MVTIESESDFYDVFEILGKGTFGEVVKSWKRSTGEMVAIKILKNDSYRSRIIKNELKLLQTMSEVDSEESHIVQFHEFFHDELKFYLVFELLEQNLFDFQKEHNFSSLPVRHIRTVTTQVLRALAKLKELSIIHADLKPENIMLVDQVRYPFRVKVIDFGSASIFNEVRYVKEPYIQSRFYRAPEILLGLPFCEKLDMWSLGCVMAELHLGWPLYPGNNEYDQIRYICETQGMPRPALLNAARKAHLFFKRSQHPEVVNSWQLKTPAEYLADTKVKSVERRKYVLKSLDQMETVNVHKMIYPDTEALAEYFDLRSMVELIKRMLTWDSHERITPSAALKHPYISTQPLKQNYDLTQYYQFCLRSLRESLPSHAKASEEETQHYSAMDEDRFYSREESAHGIQGTTSQMDELSIAEASREVPLKVWGEGPSGGGYDPLPDPAEAVANRHRVAQQSLRLRHEQAQQEPIPAYYRSRPGSPRHHKASHHVKPDPTFENLILLGQYTPEDPPSWEKESNASAASLPESGAREEASYPKGLVLSPTTQLGQSEAFEPAVPLPGPNPWLPSEDWLAEHSMERVPLKAVLHAPRNRHPLQPYLQHVASHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3Y7</accession>
    <name>A0A6I9Y3Y7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Olfactory receptor 2W6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106546088</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918338.1">
        <property type="nucleotide sequence ID" value="XM_014062863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3Y7"/>
    <dbReference type="GeneID" id="106546088"/>
    <dbReference type="KEGG" id="tsr:106546088"/>
    <dbReference type="OrthoDB" id="9039953at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="42"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013918338.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="34076" checksum="D16A8FB92BB355C6" modified="2020-10-07" version="1">MTEAKNFTSVKEFILLGLTSQRKIQLLLFGVILIIYLLTVLGNLLIIVLVQADVKLQTPMYYFLSQLAGVDICYVTSTMPLMLTQLLAEDGAISLAFCMIQIYIAVALGGVEILLFGVMAYDRYLAICRPLLYPVLMDKRCQLQCSSTCWISAPLVCVIYIVCLLCQNYCGPNQINNIICEMPMVLRLACSDTSIIEDFIFGIGSFVLLGPLTFILTSYGFILHSVLQMKSSGRLKAFSTCGSHLIVISMFYGPLLWVYIIPKTDTAADHDKQIAIFYVLITPLLNSVIYTLRNKDFHRAVAKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y544</accession>
    <name>A0A6I9Y544_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Proton myo-inositol cotransporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SLC2A13</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912820.1">
        <property type="nucleotide sequence ID" value="XM_014057345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y544"/>
    <dbReference type="GeneID" id="106541809"/>
    <dbReference type="KEGG" id="tsr:106541809"/>
    <dbReference type="CTD" id="114134"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="92"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912820.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="29160" checksum="78143F6E91AC22D3" modified="2020-10-07" version="1">MSRKANVEYTLRSLSNLMGEKRRRQPPPPPPPQPPSRREALGERSLIAAAESCSSLNSQASSSGGGGGCGDLERAARRQFQQDEPAPFVYVAAAFSALGGFLFGYDTGVVSGALLLLKKELRLDATWQELLVSSTVGAAALAALLGGALNGYWGRRACILLASLLFTAGAVLLAAAPDKETLLGGRLVVGLGIGIASMTVPVYIAEVAPPHLRGRLVTINTLFITGGQFFASVIDGAFSYLPKGGWRYMLGLSAIPAVIQFVGFLFLPESPRWLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5E5</accession>
    <name>A0A6I9Y5E5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 181 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TMEM181</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913075.1">
        <property type="nucleotide sequence ID" value="XM_014057600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5E5"/>
    <dbReference type="GeneID" id="106541999"/>
    <dbReference type="KEGG" id="tsr:106541999"/>
    <dbReference type="CTD" id="57583"/>
    <dbReference type="OrthoDB" id="28186at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015643">
        <property type="term" value="F:toxic substance binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040416">
        <property type="entry name" value="TMEM181"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054077">
        <property type="entry name" value="TMEM181_GOLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047843">
        <property type="entry name" value="WLS-like_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31918">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31918:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21885">
        <property type="entry name" value="TMEM181_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06664">
        <property type="entry name" value="WLS-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM181 GOLD" evidence="7">
        <location>
            <begin position="100"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless-like transmembrane" evidence="6">
        <location>
            <begin position="215"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013913075.1"/>
        </source>
    </evidence>
    <sequence length="521" mass="61206" checksum="C2825C772858E286" modified="2020-10-07" version="1">MEADYPAFENPLCGELKHIYQRIKETYKEIKEDLTPYKDDRYYRLAPMRLYTLSKRHFVLVFVVFFLCFGLTVLIGIAGPRVIECTSEIPPINNGSKTKSFPLSSPPVSTYNQQLWLTCTVHLEQPSDNKLDSLKTSFIMSVTIRGVLKNGSVRYYNRETHNRTRIFNCAKDCDEIIVVHLGYLNFTRYNIVVTFDKFPESYHFKSYNFTWKTYNPVFSQVEIWFRFVFVVLTFIVTCLFAHSLRKFSMRDWGIEQKWMSILLPLLLLYNDPFFPLSFLINSWFPGMLDDLFQSLFLCALLLFWLCIYHGIRVQGERKCMTFYFPKFLIVGLLWLASVTLGIWQTVNELHDPTYQYEIDTNNFQGMKIFFLVVATVYILYLLFLIIRACSELRNMPYVDLRLKFLTALTLVVLIISIAIFYLRFETQVLQDNFVAELSSHYHNSAEFLSFYGLLNFYLYTLAFVYSPSKNALYESQLKDNPAFSMLNDSDDDVIYGSDYEEMPLQNGQAIRAKFKEESDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5M5</accession>
    <name>A0A6I9Y5M5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">YIPF2</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913274.1">
        <property type="nucleotide sequence ID" value="XM_014057799.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913275.1">
        <property type="nucleotide sequence ID" value="XM_014057800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5M5"/>
    <dbReference type="GeneID" id="106542149"/>
    <dbReference type="KEGG" id="tsr:106542149"/>
    <dbReference type="CTD" id="78992"/>
    <dbReference type="OrthoDB" id="10256463at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF3">
        <property type="entry name" value="PROTEIN YIPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="95"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013913274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913275.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="34857" checksum="7F131CB80FBDEE9A" modified="2020-10-07" version="1">MAASEELNFHEFNEAMDLLAGNTDATTSSISERQSHTTLNMPSSCNEEYEPDEEDDKTELLIFQKKQSSFWTFEYYQAFFNVDTYQVLDRIKGSVLPLPGKNFVWHHLQNNPDLYGPFWICATLVFTLAISGNLSNFLEKRGSSSFHYSPQFHKVTIASIVIYCYAWLVPLGLWGYLQWRKGAHVTTDVYSFLEMVCIYGYSLFVYIPTAVLWLIPIPWLQWLLIILAIGLSGSVLVLTFWPVIRSDSKPAACAVMAIIVSLHILLAIGCKLYFFQKPSITSPTPVAYTTTTQQKHTPSFTAAEGKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6M0</accession>
    <name>A0A6I9Y6M0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Plakophilin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PKP2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914135.1">
        <property type="nucleotide sequence ID" value="XM_014058660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6M0"/>
    <dbReference type="GeneID" id="106542819"/>
    <dbReference type="KEGG" id="tsr:106542819"/>
    <dbReference type="CTD" id="5318"/>
    <dbReference type="OrthoDB" id="3245100at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002934">
        <property type="term" value="P:desmosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045110">
        <property type="term" value="P:intermediate filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF25">
        <property type="entry name" value="PLAKOPHILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="374"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="416"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="634"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013914135.1"/>
        </source>
    </evidence>
    <sequence length="782" mass="87425" checksum="98D7E72822EE8518" modified="2020-10-07" version="1">MAVPVCSPDPGYIRTVLGQQGLDEQDSSTLALPSKLSGGRAEAQKSLRIQRQIQQTLARKSKGSLINGGLHRASSVPEYVYKLDGVETDFASRPSFATSYYQSHQIGSQSSYENGWGPRSLPYNYRAMEERFHRQPLKRLEVSPESTPEKPFLFHADMRYNKALTFRQNESKRACALPPRYARSEILGYTSRSPSSRGPRQFYIEPTNETMVDYIPFSPGVPLYQRVRNSQSMSNIFEKENYPTSGAAMGQARSSMHSQVGTQHRHSSWHQSTVSKQSTKEASMSTSFSEGGGKRTTMTAAMAAAGAKGIVEQERMSAGRCQIGNPEAEMNLERAVHILQSENPLSPRVLSAIVFIQHESFQRAEARRKVYSLVGIPKLLELLNVQNEEVQRAACGALRNLVYEDNDNKLEVSERKGISILLRLLRQTRDVETKKQITGLLWNLSSNDQLKNVLIREALQPLTETILIPYTGWPDRDYPKSSIIPDPDIFYNATGCLRNMSSAGPEGRKKMRECDGLIDSLVYYIQGTTADRKPDDKATENCVCVLHNLSYQLEDELPNHYTEKLYVPKGDDPSRNNIGCFGTRSRKAKQVTEMNVMGVNMVVGALSIFSLECGHIIEIKMPFGVAHIIVKKANGLPNIISMLHVSNVSVRKTAVSLLRNLSRNTLLHNEIARVLLPELMAMLPNYVPTSNVDNDIAASACYALYNLTQNSSSNARLLLNNDGVSKVINISQSDNKSGRAASILLYSLWSHTDLHSAYKKAGYKKLDFINNRTLRAYNLLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y745</accession>
    <name>A0A6I9Y745_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Protein FAM92A1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">FAM92A1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
            <location evidence="3">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the CIBAR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914560.1">
        <property type="nucleotide sequence ID" value="XM_014059085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y745"/>
    <dbReference type="GeneID" id="106543162"/>
    <dbReference type="KEGG" id="tsr:106543162"/>
    <dbReference type="CTD" id="137392"/>
    <dbReference type="OrthoDB" id="60621at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07598">
        <property type="entry name" value="BAR_FAM92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000047">
        <property type="entry name" value="protein FAM92A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035590">
        <property type="entry name" value="BAR_CBAR1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009602">
        <property type="entry name" value="CBAR/FAM92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21223:SF4">
        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21223">
        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06730">
        <property type="entry name" value="FAM92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="12 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="265"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="112"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="271"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013914560.1"/>
        </source>
    </evidence>
    <sequence length="293" mass="34025" checksum="64E343C48845FF33" modified="2020-10-07" version="1">MLMLGRGLDARDNQTRQIREAVSNVEKHFGELCQIFAAYVRKTARLRDKADLLVNEIHAYAATETPNLKFGLKNFADEFSKLQDYRQAEVERLEAKVVEPLKCYGTIIKLKREDLKATLTAKNREAKQLSQLERTRQRNPSDRHIISQAESELQRASLDATRTSRQLEETIDNFEKQKIKDIKNILSEFITIEMLFHGKALEIYTAAFQNIQNIDENEDLEVFRSSLYPPEHQTRLDIVRANSKSPLQRTGSSRTCTGIVQQIARNHLKEDEAEEEEEEEEEDEELETTKEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y800</accession>
    <name>A0A6I9Y800_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein C2orf42 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106544341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916060.1">
        <property type="nucleotide sequence ID" value="XM_014060585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y800"/>
    <dbReference type="GeneID" id="106544341"/>
    <dbReference type="KEGG" id="tsr:106544341"/>
    <dbReference type="OrthoDB" id="6506929at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026049">
        <property type="entry name" value="C2orf42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029269">
        <property type="entry name" value="Zf-tcix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13518:SF1">
        <property type="entry name" value="C2ORF42 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13518">
        <property type="entry name" value="PUTATIVE TREBLE-CLEF ZINC-FINGER C2ORF42 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14952">
        <property type="entry name" value="zf-tcix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative treble-clef zinc-finger" evidence="2">
        <location>
            <begin position="16"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916060.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="62494" checksum="E00DF2EA26862ED8" modified="2020-10-07" version="1">MESGSLRSKVPAFLSDLGKATLRGIRKCPRCGTYNGTRGLSCKNKTCGTVFRYGSRKPAGVDAVKIITGSDLQVYSVRQRDRGPEYRCFVELGVSETTVQTVDGTILTQLSSGRCYLPACLKAAAQGVGENQCQHLKLAMGCQAEATPLTLKSSVLNAMQASAQTKQALWELATQPTGPLVQRVTKNVLVVKGKAGPKHSLGYLHITVAPRSAHTHLAGPRFFCPCQSSRASKASPPAQEGSQKCLHFFACLCAFASDEALAQEFGDFLSDEPDAAGLKSPARAPPACQPDSPLPSGDPAASKAKRKKKDEGLGLQASSPLLSPEVASSSLQKNGLKRAPVTSLKRQTGIQGLEESQVALSFLEWLASVTERLHQAMHYQFDGKPEPLVFHIPQAFFEALQQRIGLGSVKMRLPNSTTAFVRKDALPLGTFSKYTWHITNILQVKQIFDTVEMPLEITRSFIQNRDGTYEPFKCPKVEVESIAETYGRLEKQPVLRPLELKTFLKVGNTSPDQKEPTPFIIEWIPDILPQSKIGELRIKFEYGHYRNGHPADYQEQRPSLEQALEIITFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y808</accession>
    <name>A0A6I9Y808_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106544373</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106544373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916100.1">
        <property type="nucleotide sequence ID" value="XM_014060625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y808"/>
    <dbReference type="GeneID" id="106544373"/>
    <dbReference type="KEGG" id="tsr:106544373"/>
    <dbReference type="OrthoDB" id="9012254at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916100.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="44351" checksum="40AF126D1C0E9F4C" modified="2020-10-07" version="1">MPHTPRQRLQPRAPIWSETETRDFLALWGELLILRWIENRPPNSDIYEDLSAQMIARGHERNASQCRNRARDLKRSYRRAKDAQICAGAEPVSCRYFQELDQMFGGEMDRATNRSLASMDGSVRVVVKREDAKDMEAQASEEESNQMTPPNTLSSMEADTDTETEPNVGITRGLVTVVDPNVIENTIVEDGPEEKVVPFCGQPVPGCSEDGHHTATGRGRREKSFGHPLTTAERMASMRERKRRSHEEMMHEIMTDYRRTWETMEALHERSEINAGDFREAVLREMHLDREARTREAQLDRESWAREAQLDREARIRDNHLEREIRIKELGMIEAELQRTREILHPMVDAVASVAEALRVASRILPPSQPAQKMRAGVFLESKESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8L6</accession>
    <name>A0A6I9Y8L6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ankyrin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106545188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917140.1">
        <property type="nucleotide sequence ID" value="XM_014061665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8L6"/>
    <dbReference type="GeneID" id="106545188"/>
    <dbReference type="KEGG" id="tsr:106545188"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF194">
        <property type="entry name" value="INVERSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CARD" evidence="4">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="262"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="292"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="325"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="416"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="449"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="482"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="515"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="548"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="614"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="649"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="682"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013917140.1"/>
        </source>
    </evidence>
    <sequence length="750" mass="82608" checksum="A9BE2BD18C42DF0D" modified="2020-10-07" version="1">MYSNLFTNPYATEVLKMKKNELVEGIKNPDHLLDCLIEKSILSPEKKMVLSYYRTRMAKNSCILDILASQGERACRLFFYPCLKEVEPRLYSNIRNYVSNVNETVGDARRQLIGYLLEKDKGSIQKDKASHQGKQASPRSIKPKKMIYNKPKSKSTLAARQSLDNVPTVFDLVRKGTLSDLEKALNPSNVNATNSANETLLHIAAAHGHTEIIDYLISKGAKLEVKDNKGRTPLHRAAEKGHAKAVNRLLQAGANMYSLDQEGKTPLHLANRNQHTDVLKNILKEEARRHRNQHNFLHMAALKDDSDLVQVLLKNGALVDARDERGQTALSYAVSQGHEKTVKVLLEGGAKVDSSIIEAAFNSNNQSLFKLLLEYARELSSESMVSSLFKAIEKDLHGIVAALIDRGTDVNVRNEEQYTPLLMACKTGKIKSAKVLIEKGANLKDKAPNLSSPLHLVVEAGAFSIAQMLLQKGIDPNITAQGNQTPLHVAAIYNRGALVDLLIEGGAKIDAVTTDLFTPLHLASEKGHTDVALKLLQHKANVHLKNKRSKTPLHLAAEMGNPAMVEMLLNFKADPNATDKEKKCPLHFATLGGHFYAVKALLAKKSKVASKDMDGCTPMHYAALSGNVEILKELLVTGNNKNINDKNIWRKTLLHLAAEHGHSNLIDFLLSNGSAINALDNNKDTPLHCACKAGHFDSVRALLNWSAGDKANLQATNSLKKTPLQVAESSVTDQQAQIVNLLKKKMLLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9K4</accession>
    <name>A0A6I9Y9K4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 532</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106546479</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918830.1">
        <property type="nucleotide sequence ID" value="XM_014063355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9K4"/>
    <dbReference type="GeneID" id="106546479"/>
    <dbReference type="KEGG" id="tsr:106546479"/>
    <dbReference type="OrthoDB" id="8856548at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001446">
        <property type="entry name" value="Zinc finger protein 532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000797">
        <property type="entry name" value="zinc finger protein 592 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045914">
        <property type="entry name" value="Zn532-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041697">
        <property type="entry name" value="Znf-C2H2_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN 532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222">
        <property type="entry name" value="ZINC FINGER PROTEIN 532-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16622">
        <property type="entry name" value="zf-C2H2_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="247"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="335"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="365"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="395"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="513"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="574"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="284"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="539"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="559"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013918830.1"/>
        </source>
    </evidence>
    <sequence length="611" mass="68800" checksum="C69D8E09BC8F0E94" modified="2020-10-07" version="1">MIASPTSNSSVTSSILQNPTGVGTHTITKIQSSITGTVISAPSSTPVPPAMPLDEDSSKLCRHSLKCLECNEVFQDETSLATHFQQAADSSGQKTCSICQMLLPNLCSFASHQRIHQHKSPYTCPECGAICRSVHFQTHVTKNCLHYTRRVGFRCVHCNVVYSDVAALKSHIQGSHCEIFYKCPICPMAFKSAPSTHSHAYTQHPGIKIGEPKIIYKCSMCDTVFTLQPLLYRHFDQHIENQKVSVFKCPDCSLLYAQKQLMMDHIKSMHGTLKSVEGPPNLGINLPLSTKPSIQNSVSHNKEDSKSINGTEKAEKNPPTSVKKAEPKKGSHPGWTCWECDRLFTQRDVYISHMRKEHGKQMKKHPCRQCDKSFSSSHSLCRHNRIKHKGIRKVYTCSHCPDSRRTFTKRLMLEKHIQLMHGIKEPDVKEMAESTNMGESSVKEDTKVPSPKRKLEEPVLEFRPPRGAITQPLKKLKINVFKVHKCAVCGFTTENLLQFHEHIPQHKSDGSSYQCRECGLCYTSHVSLSRHLFIVHKLKEPQPVSKQNGSGEDSQQENKPSHEEESTDRSTSDRKCKVCAKTFETEAALNTHMRTHGMAFIKSKRMSSSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9T6</accession>
    <name>A0A6I9Y9T6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Complement component C6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC106546796</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919240.1">
        <property type="nucleotide sequence ID" value="XM_014063765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9T6"/>
    <dbReference type="GeneID" id="106546796"/>
    <dbReference type="KEGG" id="tsr:106546796"/>
    <dbReference type="OrthoDB" id="9867095at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048831">
        <property type="entry name" value="C8A_B_C6_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF4">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21195">
        <property type="entry name" value="EGF_C8A_B_C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="12" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="17 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="19" id="KW-1053">Target membrane</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027081592" evidence="21">
        <location>
            <begin position="23"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="22">
        <location>
            <begin position="177"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013919240.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="63893" checksum="345C0E8D4E7AE999" modified="2020-10-07" version="1">MKKAYNWLFLILLLAAIENSAGCYCDHYPWTAWSGCSRTCNYGTQNRYRQIKVDDYYQKNFCAQICTTQESRACNQQACPINCRLGDFGSWSECDPCVKKQFRTRSFTQLAQFKGQPCNEPLVESRPCFPTKLCNMEETNCGNKFHCENGRCISQKLKCNGENDCEDNSDERNCPRVRPVCARKHESIPGVDLMGYGYHILAGTRRGNVLHNSFNGGTCRLVKTSDLRKTYRLPANVESVFFQVVNEEDEVASEYYNDLIPFESHTSDSGSSTSSSKRRSGIPVLFSKKTKIRVTSSYSFKEAIQASKKKESRFIRIHKVIAVSNFTMKESGLQPSKVFLEALNSLPLEYNYPLYSRIFDDFGTHYYSSGSLGGRYDLLYQYSAEELRNSGLTQAESQECVRTETTRRILWKKKKKVRISCTSNRMAARHEGSFLQSSEKSISFTKGGRSQFAAALAWEGRGAFPEDTIFTNWLESMKDNPTVIDFELTSILELVKNIRCAATKRSNLRRALSEYAAQFDPCQCAPCPNNGKAVLSGTECLCVCRAGTYGDNCEIRTPDYHSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBF9</accession>
    <name>A0A6I9YBF9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mitogen-activated protein kinase kinase kinase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MAP3K2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_013921435.1">
        <property type="nucleotide sequence ID" value="XM_014065960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBF9"/>
    <dbReference type="GeneID" id="106548568"/>
    <dbReference type="KEGG" id="tsr:106548568"/>
    <dbReference type="CTD" id="10746"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06405">
        <property type="entry name" value="PB1_Mekk2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06652">
        <property type="entry name" value="STKc_MEKK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000071">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000026">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034879">
        <property type="entry name" value="PB1_MEKK2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="43"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="232"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013921435.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="55585" checksum="E02DE3DA864EC935" modified="2020-10-07" version="1">MDEQQALNSIMQDLAVLHKASRPALPLQEMGKGKLISPKKQNDVRVKFEHRGEKRILQFSRPIKLEDLASKAKVAFGQSMNLYYSNNELVIPLAIQDDLDKAVELLDRSVHLKSLKILLVAHGNTQNNMDPLSSLEDLDNTVFRVTEKKNRNSVIGRKTFPRVRRTQGNCLRTPVSFSPTDYSLSTSSGSSIFTPEYEDGRMRRRGSDIENPTLTVMDISPPSRSPRAPTNWVLGKPLGQGAFGRVYLCYDADTGRELAVKQVEFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDHPEGTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICLSGTGMKSATGTPYWMSPEVISGEGYGRKADIWSVGCTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPQLPPHVSDHGRDFLKRIFTEAKLRPSADELLRHTFAHYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBG1</accession>
    <name>A0A6I9YBG1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Membrane-associated transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLC45A2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921419.1">
        <property type="nucleotide sequence ID" value="XM_014065944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBG1"/>
    <dbReference type="GeneID" id="106548551"/>
    <dbReference type="KEGG" id="tsr:106548551"/>
    <dbReference type="CTD" id="51151"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17313">
        <property type="entry name" value="MFS_SLC45_SUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF34">
        <property type="entry name" value="MEMBRANE-ASSOCIATED TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="495"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013921419.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="57672" checksum="D45A9AF53F041C5E" modified="2020-10-07" version="1">MGPLSRKEETVFKPLTGKDPFKPMKPSKKRTGQLIMHSTVMFGREFCYAVEAAFVTPVLLTVGLPKSLYSFVWLISPILGFLLQPVVGSVSDNCTSRWGKRRPFIFILGLLMLLGMSLYLNGDAVISVLVIDRGTWKTWAIIITMLGVVLFDFAADFIDGPIKAYLFDVCTHQDKQKGLHYHALLTGLGGALGYLTGAIDWGATILGKYLGSEFHVMFFFGALVFLLCLTVHLCSIPEAPLAHTSDETEVLVKNSQADQYGAIESVKNNSEYGEMQISPKEEKLTTKADIQVQRQITIMSLLEALLHMPPHYRSLCLSHLMGWTAFLSNMLFFTDFMGQIVFEGDPYAPHNSTAYLTYKRGVEIGCWGLCINAISSSVYSYVQRALLPFLGLKGLYFVGYLLFGLGTGFIGLFPKVHPTLTLCSLFGVMSSTLYTIPFNLVTNYHKEELNQKHDEVADSSRGKGIDCAALTCMVQLAQIIVGVGLGLLVSAAGSVVVVVISASAVSLLGSCFVALFVRYVDESNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC80</accession>
    <name>A0A6I9YC80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">General transcription factor 3C polypeptide 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Transcription factor IIIC 35 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Transcription factor IIIC subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GTF3C6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts with GTF3C4 and GTF3C5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TFIIIC subunit 6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921669.1">
        <property type="nucleotide sequence ID" value="XM_014066194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC80"/>
    <dbReference type="GeneID" id="106548748"/>
    <dbReference type="KEGG" id="tsr:106548748"/>
    <dbReference type="CTD" id="112495"/>
    <dbReference type="OrthoDB" id="1877767at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4370:FF:000001">
        <property type="entry name" value="general transcription factor 3C polypeptide 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042771">
        <property type="entry name" value="GTF3C6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019481">
        <property type="entry name" value="TFIIIC_triple_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21860:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21860">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10419">
        <property type="entry name" value="TFIIIC_sub6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Transcription factor TFIIIC triple barrel" evidence="12">
        <location>
            <begin position="26"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="162"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013921669.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25638" checksum="FE8B94C04F8A119F" modified="2020-10-07" version="1">MAAAASREAGGGPGSMEAADGTQQEEEVEEQLVVVELSGIIDSDFLEKCDNKCKILGIDTERPILQIDRYIFAGEYEDTLGTCVVFEENHDHDVEGDQKLQLKYKCHTMKKLKMTRTLLIEKKEGEENIGGGIEWLDLKDKDFSYSRPNMICNFLYEKDDETAESQDKLAEESEEEASDQGNMDWDLEPGITADIAKDDAEPLLDTPDPITENPSIENTQNMASEEPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCL9</accession>
    <name>A0A6I9YCL9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endophilin-B1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">SH3 domain-containing GRB2-like protein B1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SH3GLB1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921903.1">
        <property type="nucleotide sequence ID" value="XM_014066428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCL9"/>
    <dbReference type="GeneID" id="106548928"/>
    <dbReference type="CTD" id="51100"/>
    <dbReference type="OrthoDB" id="14167at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11945">
        <property type="entry name" value="SH3_Endophilin_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000028">
        <property type="entry name" value="endophilin-B2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028503">
        <property type="entry name" value="SH3GLB_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF52">
        <property type="entry name" value="ENDOPHILIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="16">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="277"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013921903.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="37494" checksum="467869C61E5F8AB2" modified="2020-10-07" version="1">MKQTEVLLQPNPNARIEEFVYEKLDRKAPSRLNNPELQGQHMIDAGNEFGPGTAYGNALVKCGETQKRIGAADRELIQTAAINFLTPLRNFIEGDYKTITRERKLLQNKRLDLDAAKTRLKKAKVAEARALQLNLSPPDENNIMVNVSYMLNLLHVKWLKSEQEVRIAQSEFDRQAEITRLLLEGINSTHAHHLRCLNDFVEAQLTYYAQCYQYMLDLQKQLGSFPATVLANNNQSSSTCIPSSSCSNTSSATVTPSLTSINSAAASSSFGELKPSSGSRKARVLYDYDAANSSELSLLADEVITVYSVPGMDSDWLMGERGNQKGKVPITYLELLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFV1</accession>
    <name>A0A6I9YFV1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Runt-related transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RUNX1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922795.1">
        <property type="nucleotide sequence ID" value="XM_014067320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFV1"/>
    <dbReference type="GeneID" id="106549617"/>
    <dbReference type="KEGG" id="tsr:106549617"/>
    <dbReference type="CTD" id="861"/>
    <dbReference type="OrthoDB" id="10029800at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016513">
        <property type="term" value="C:core-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002062">
        <property type="term" value="P:chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Runt-related transcription factor"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Signal Protein Aml-1b, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000040">
        <property type="entry name" value="AML1_Runt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="Runx_central_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013711">
        <property type="entry name" value="RunxI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016554">
        <property type="entry name" value="TF_Runt-rel_RUNX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950">
        <property type="entry name" value="RUNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950:SF40">
        <property type="entry name" value="RUNT-RELATED TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00853">
        <property type="entry name" value="Runt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08504">
        <property type="entry name" value="RunxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009374">
        <property type="entry name" value="TF_Runt-rel_RUNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00967">
        <property type="entry name" value="ONCOGENEAML1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51062">
        <property type="entry name" value="RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Runt" evidence="9">
        <location>
            <begin position="51"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>chloride</name>
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        <location>
            <position position="171"/>
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        <ligand>
            <name>chloride</name>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
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        <source>
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            <dbReference type="PIRSR" id="PIRSR009374-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51062"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922795.1"/>
        </source>
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    <sequence length="460" mass="49853" checksum="FA1D48FC73DC3CCB" modified="2020-10-07" version="1">MRIPVDTSTSRRFTPPSTTLSPGKMSDALPLVGQENGSSALVGKLRNADRSMVDVLADHPGELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHRQKLDEQTKTGSLSFSERLSELEQLRRTAIRVSPHHPAPAPNPRASLNHTTAFNPQPQSQIQDTRQLQPSPPWPYDPSYQYLGSIATSSVHPATPISPGRASGMTSLSAELSSRLTSASDLTAFSDPRVGIDRPFSAIPSISDPRMHYPGAFTYTPTPVSSGIGIGMSAMTTATRYHTYLPPPYPGSSQAQGSPFQTSSPSYHLYYGTSAGSYQFSMMTGGDRSPPRILPPCTNASTGSTLLNPNLPNQNDVVDTEGSHSNSPTNMGTTARLEEAVWRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG74</accession>
    <name>A0A6I9YG74_9SAUR</name>
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        <submittedName>
            <fullName evidence="5">Lecithin retinol acyltransferase</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">LRAT</name>
    </gene>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:rough endoplasmic reticulum"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047173">
        <property type="term" value="F:phosphatidylcholine-retinol O-acyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042572">
        <property type="term" value="P:retinol metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006776">
        <property type="term" value="P:vitamin A metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
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        <property type="entry name" value="LRAT"/>
    </dbReference>
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        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46678">
        <property type="entry name" value="LECITHIN RETINOL ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46678:SF1">
        <property type="entry name" value="LECITHIN RETINOL ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
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    </dbReference>
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        <property type="entry name" value="LRAT"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="262"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="LRAT" evidence="3">
        <location>
            <begin position="117"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR642288-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923273.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="33429" checksum="66B004F91E748A12" modified="2020-10-07" version="1">MRGLCTVASPPLPESQSLMFSRNCPLACLPIKADSVCGMAQLAILKRDQTRINQEQLVQQEKKIMKNPMYEIASLLLEKLLLLANLKVLQTGSSGEEEKRKKDVCWYDSSYFTPGDLLEVPRTLFIHFGIYLGDNQVAHLMPDILPAFTNDQKLIQKVVTNKRLILGVITKMARIRVDTVEDFAYGGSILVNNMDSLFKNCILCNDEVVHRAKKLVGTTEYSLLWNNCEHFVTFCRYGSPVSFQTTKFCETVKMIIRDQRSVLVSALLGLISILYLGLGPSTTLPTIIIPFMLWMAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG81</accession>
    <name>A0A6I9YG81_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Src kinase-associated phosphoprotein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Src family-associated phosphoprotein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SKAP2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923278.1">
        <property type="nucleotide sequence ID" value="XM_014067803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG81"/>
    <dbReference type="GeneID" id="106550020"/>
    <dbReference type="KEGG" id="tsr:106550020"/>
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    <dbReference type="OrthoDB" id="243840at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042113">
        <property type="term" value="P:B cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12045">
        <property type="entry name" value="SH3_SKAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000194">
        <property type="entry name" value="Putative src kinase-associated phosphoprotein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000097">
        <property type="entry name" value="Putative src kinase-associated phosphoprotein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037781">
        <property type="entry name" value="SKAP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15129:SF2">
        <property type="entry name" value="SRC KINASE-ASSOCIATED PHOSPHOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15129">
        <property type="entry name" value="SRC-ASSOCIATED ADAPTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0075">B-cell activation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="108"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="284"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="54"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923278.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="39041" checksum="EC3CF58F12220B41" modified="2020-10-07" version="1">MMQDLPAIPEEISNFLADVDTFISNILKGENLSKRAKEKKDVLVKKMKDIKSNYPQEFPEIGGDDEGEENERSCPAAPDSLSLACDKEDEGPSDGHQFPPIAAQDLPFVSVSGYLEKRRKDHSFLGFEWQKRWCALSKTTFYYYGSDKDKQQKGGFAIDGYTVRMNNTLRKDGKKDCCFEIAAPDKRIYQFTAATPKEAADWVERLKFVLQDMGSLTIPEEEYDDIEENSIAPSQSGGAPIFDDIYEELPEDAATVAEMPGKSEESKKQSQDAVSSISGNKKTDYVNLYQGLWDCAGDVPDELSFKRGDVIYILSKEYNRFGWWVGEMKGTIGLVPKAYIMEMYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGX2</accession>
    <name>A0A6I9YGX2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cathepsin D</fullName>
            <ecNumber evidence="4">3.4.23.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CTSD</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acid protease active in intracellular protein breakdown.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.</text>
            <dbReference type="EC" id="3.4.23.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.5" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013923130.1">
        <property type="nucleotide sequence ID" value="XM_014067655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGX2"/>
    <dbReference type="GeneID" id="106549898"/>
    <dbReference type="KEGG" id="tsr:106549898"/>
    <dbReference type="CTD" id="1509"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000009">
        <property type="entry name" value="Aspartic proteinase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000066">
        <property type="entry name" value="Napsin A aspartic peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF42">
        <property type="entry name" value="CATHEPSIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 17" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026946367" description="Cathepsin D" evidence="18">
        <location>
            <begin position="19"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="19">
        <location>
            <begin position="76"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="315"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013923130.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="43365" checksum="860290A0FEA58ACD" modified="2020-10-07" version="1">MGLRDLILFASLVAACSALIRIPLKKFPSMRSIYNEYGTNIQDLIELGQMLKYKFGGPGAEIPTPEALKNYMDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSTHCGMLDIACLIHHKYDSSKSSTYVKNGTDFAIHYGTGSLSGYLSQDTVTIGDLCVKDQLFGEATKEPGITFIAAKFDGILGMGYPKISVDKVAPFFDNVIKQELLEKNIFSFYLNRDPKGQTGGELLFGGTDSQYYSGDFNWVNVTRKAYWQVHMDKVDVANGLTVCPDGCEAIVDTGTSLITGPTKEIKELQKAIGAKPIIKGQYMLQCDKLSTLPAVSFVLGGQSYALTADQYALKVTVQGETLCLSGFSGLDVPPPGGPLWILGDVFIGSYYTVFDRDNDSVGFAKSTGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHC2</accession>
    <name>A0A6I9YHC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat-containing protein KIAA1875-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106550239</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923582.1">
        <property type="nucleotide sequence ID" value="XM_014068107.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550239"/>
    <dbReference type="KEGG" id="tsr:106550239"/>
    <dbReference type="OrthoDB" id="6262491at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45532">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45532:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="254"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="654"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="926"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="952"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1317"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1332"/>
            <end position="1363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013923582.1"/>
        </source>
    </evidence>
    <sequence length="1377" mass="154666" checksum="E0D530BE84649A41" modified="2020-10-07" version="1">MVESSGSSSSRVGQRKRPHQQSPPAEEEMKKEQGAPLRAGQARGRRLWALLRRGFRRAVEKMKNEEMKTLHLSHGLQHQCRIVLPSPVRHVTHDLQRRAFVVLDAANTLHLLTEDGCCRESVKGLAPMTGILYASHVNQFVAWFTGGLQVLDSNFQLLSQVPSSLPIRCGIYSKQLNRIVTAGDGNLTLWSFRYGSRSLQCRASVSTGLGPTDLFVRLALDTGSLSEPHRCFASCATGVATFDISRGSLLAFSTKLHSREITDLAYCEAIGCVITASRDTTIKVWNKEWHIQIVFVGHTAPVVAVSIYPRRPLIFSASQDGTIRTWNLNTIDQVDQVHVSEPVESLDTHTSSHVFSISGCTLNLWKINQLYSLYTALGSPVKRLSCIDLSLLGNFPARILCLCRDSTVQLLEAQSGAPVSILSLDQYSPARDVAYCLPRETLFVLLEQGQVLRVNAATSPMTVKRSFSSSFWDSKPCCILLYSHVVEAEKVQATWLEVIQNQGYRKNWQKSTINMQDRNRFLPILGHKNGSLSVIDWFSGRVQYTVEAHNPERVTALAEYTTQICVLSAGADLTVKMWRLFPYTEESLLLLLCISCASPAWHMCFLGETLAVAFQDPETVTYSIVHYNLMEQTRSEHGPEDDAQDDITGLCCCPNLQLFASSSRDGSVKIWDHKNKLIRHLKLNTIPESLAFANHKGDLLVGLEQHLYLIPHTQYLPSYYQMQLLWAKFLEPLQDVSLPMSPSTFEALVQENDRRLRQELPVEKSESSVSSTFQVPLKESKEVREARLKQEGIAQVAERNRDLQLLQEGKLSRAKRGPLSKEMQEEAFRRYLGFFYKEQLNLQIPEEDPFDADEVLESLAQMDPLSALRGPAISRMFLGGFTKPRSLEMGSSLFVDISPSLRCTSSLAVPGSLQVKRKKVEICAGPSSAQMQETPPLGKAGAAPTQEAPEALRPLSVGGSRQGFQPRQRGPEEEEAAMPAERLSPVKTLGSLVSVTSETPSVPSPTRHFKSEKELKSLQLPKISSGFIPNSVVVQQLHTADLLEGEKLVKVIGPQKVSQSSSKASSVWTFEEDEVSEEEPWLVGQESSSKSWIRPAKEDAPSTKQSLPGVFLTQLDESQYAEPQEALPAFVLPFVGQEWFQNLFPEGVRPEMPLHALVRKLQQSLVTSDFHIKTEVLGAITYLEDQLEDKAKRWIQYTLFEVLNQEGNTPSLQEKSQEQFILAALRVLLDLDKDSLDLMVELMCCYLLAAPYARAVFKRMFQELGLQDPHNFFFKEMNSWMVEMGDPKEAVRKLSREWLEEKIRIFKEHRTRQWEEEAVSGKQLPLDTSHKIQGRKRLEKSHKKPRKQAGKLAKAAHKSHHTPRAPQTLGHFRSGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI85</accession>
    <name>A0A6I9YI85_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Structural maintenance of chromosomes protein 1A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923545.1">
        <property type="nucleotide sequence ID" value="XM_014068070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI85"/>
    <dbReference type="GeneID" id="106550207"/>
    <dbReference type="KEGG" id="tsr:106550207"/>
    <dbReference type="OrthoDB" id="9397772at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030893">
        <property type="term" value="C:meiotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF170">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923545.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="15556" checksum="B9331E04603B57E0" modified="2020-10-07" version="1">MNQLQQKLNEQQSILQRIAAPNMKAMEKLESVRDKFQETSDEFEAARKRAKKAKQAFEQIKKERFDRFNFCFESVATNIDEIYKALSRNSSAQVRMPYLFRFAWYQGDCVISKVLTFDLTKYPDANPNPNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIE7</accession>
    <name>A0A6I9YIE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Iron-sulfur cluster co-chaperone protein HscB, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HSCB</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HscB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923976.1">
        <property type="nucleotide sequence ID" value="XM_014068501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIE7"/>
    <dbReference type="GeneID" id="106550568"/>
    <dbReference type="KEGG" id="tsr:106550568"/>
    <dbReference type="CTD" id="150274"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.20:FF:000002">
        <property type="entry name" value="HscB mitochondrial iron-sulfur cluster co-chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="entry name" value="HscB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021:SF15">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="HSC20 (HSCB), C-terminal oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="67"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013923976.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="24852" checksum="5D064A444C55F523" modified="2020-10-07" version="1">MGSQRRHASRASSSSPPRQALRLRDRGRGARLPGCRSGCGGGAPLGSAGPAFCPSCRALQPPEPRADLFGVLGCPRGFSLDLARLRQRFLALQRSLHPDRFSRRAQAERGFSEQHAALVNLAYHTLRSPLSRGLYLLELRGVELAAGSDAEAEPGFLAEVLELNEQLAAAESEASLAGLERLLAAKQAALVQELSGAFERDDLPEAKRLLGKMKYFANLEEKVKEKKGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIR7</accession>
    <name>A0A6I9YIR7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microsomal glutathione S-transferase 1</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MGST1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16438"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer; The trimer binds only one molecule of glutathione.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPEG family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013924096.1">
        <property type="nucleotide sequence ID" value="XM_014068621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIR7"/>
    <dbReference type="GeneID" id="106550677"/>
    <dbReference type="KEGG" id="tsr:106550677"/>
    <dbReference type="CTD" id="4257"/>
    <dbReference type="OrthoDB" id="193139at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.550:FF:000002">
        <property type="entry name" value="Microsomal glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040162">
        <property type="entry name" value="MGST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689:SF6">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013924096.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16630" checksum="9ED3F5438E5E1C69" modified="2020-10-07" version="1">MGNFHELLDHEVTLAFMRYGTIVVLKMMLMSTITAFFRMKKKVRDESWSYEKKQCVDSFPRIHLNDLENIIPFLFAGFFYSFSGVSLSTALLHYRIFLGSRIFHTIAYLVPLPQPSRGLAWMAGYLVTFSMMYKVIMIAFTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJG9</accession>
    <name>A0A6I9YJG9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Signal transducing adapter molecule 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">STAM2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923925.1">
        <property type="nucleotide sequence ID" value="XM_014068450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJG9"/>
    <dbReference type="GeneID" id="106550520"/>
    <dbReference type="KEGG" id="tsr:106550520"/>
    <dbReference type="CTD" id="10254"/>
    <dbReference type="OrthoDB" id="10068368at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21390">
        <property type="entry name" value="GAT_STAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11963">
        <property type="entry name" value="SH3_STAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16999">
        <property type="entry name" value="VHS_STAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000009">
        <property type="entry name" value="Putative signal transducing adapter molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1940:FF:000002">
        <property type="entry name" value="Signal transducing adapter molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000086">
        <property type="entry name" value="signal transducing adapter molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050670">
        <property type="entry name" value="STAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035675">
        <property type="entry name" value="STAM2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047493">
        <property type="entry name" value="VHS_STAM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929:SF1">
        <property type="entry name" value="HEMATOPOIETIC LINEAGE CELL-SPECIFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="15">
        <location>
            <begin position="16"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="204"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="260"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923925.1"/>
        </source>
    </evidence>
    <sequence length="515" mass="57132" checksum="67EA84D7E1D76BEC" modified="2020-10-07" version="1">MPLFAPNPFEQDVEKATNEYNTVEDWAVIMDICDKVGSTPNGAKDCLKAILKRVNHKVPHVALQALTLLGACVSNCGKIFHLEICSRDFASEVRVIINKAHPKVCDKLKALMVEWSEEFQKDPQFSLISATIKSLKEEGIVFPTAGTQSPSAVAKNGASSGKNKEEEDIAKAIELSLQEQKQQQQQTETKSLYPSVDTQHSHQKLSRKVRALYDFEAVEDNELTFKSGDIIVVLDDSDANWWKGENHRGMGLFPSNFVTPNLNDEPETVPMDKDASEDTTEITKPEPEPVYIDENKMDKTLQLLQSIDPQDPKSDSPDLLDLEDVCQQMGPMIDEKLEEIDRKHSELSELNVKVMEALELYNKLMNETPMYSAYSKMHSTAQYAPTSASVPVQSYPIHPHSGNYVVHGMPQSYNPGSDQIGSLRSLPPTMNSSVTSQPAPTYMSPGLDSVPSSTYMNQNSCLPPAGPVSYAQPMTMDMLAYQNTASSLPQGPCYPAVPTHSLPQQQTNYHQQPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK05</accession>
    <name>A0A6I9YK05_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cadherin-related family member 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106550676</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924095.1">
        <property type="nucleotide sequence ID" value="XM_014068620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YK05"/>
    <dbReference type="GeneID" id="106550676"/>
    <dbReference type="KEGG" id="tsr:106550676"/>
    <dbReference type="OrthoDB" id="9047765at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF126">
        <property type="entry name" value="PROTOCADHERIN FAT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="6">
        <location>
            <begin position="41"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013924095.1"/>
        </source>
    </evidence>
    <sequence length="181" mass="20397" checksum="93FBF74E8C724B43" modified="2020-10-07" version="1">MELQVLTVQVTDINEPPMFLGNLANQTVIIYILEKRLPEDLYQVHAGDPEKTPPKNVSISLSSDSTFFEISPTGTITTWKTFDFEKDPPSYTLNVTIKDSLGVSVKRTLMVKIINVIDRDPYFVTKENVFKIPEEAPANMFVANITAKDPEVEDFIRSLRYSMGAPEAIPKFSINPSKELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLV8</accession>
    <name>A0A6I9YLV8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KCTD10</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BACURD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925116.1">
        <property type="nucleotide sequence ID" value="XM_014069641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLV8"/>
    <dbReference type="GeneID" id="106551519"/>
    <dbReference type="KEGG" id="tsr:106551519"/>
    <dbReference type="CTD" id="83892"/>
    <dbReference type="OrthoDB" id="2333377at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18399">
        <property type="entry name" value="BTB_POZ_KCTD10_BACURD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000013">
        <property type="entry name" value="BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF14">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="32"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013925116.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="35504" checksum="8DCCC753399928C3" modified="2020-10-07" version="1">MEEMSGETVVSTAVPAATTRTTSFKSGSPSSKYVKLNIGGALYYTTMQTLTKQDTMLKAMFSGRMEVLTDSEGWILIDRCGKHFGTILNYLRDGAVPLPESRREIEELLAEAKYYLVQGLVDEGQAALQNKDVYEPFCKVPIITSSKEEQKLIATSNKPAVKLLYNRSNNKYSYTSNSDDNMLKNIELFDKLSLRFNGRVLFIKDVLGDEICCWSFYGQGRKIAEVCCTSIVYATEKKQTKVEFPEARIYEETLNILLYEAQDGRGPDNALLEATGGAAGRSHHLEEDEERERIERVRRIHIKRPDDRTHMHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLY2</accession>
    <name>A0A6I9YLY2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 171</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCDC171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924805.1">
        <property type="nucleotide sequence ID" value="XM_014069330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLY2"/>
    <dbReference type="GeneID" id="106551266"/>
    <dbReference type="KEGG" id="tsr:106551266"/>
    <dbReference type="CTD" id="203238"/>
    <dbReference type="OrthoDB" id="287623at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038820">
        <property type="entry name" value="CCDC171"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47899">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 171"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47899:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 171"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924805.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="46670" checksum="7FB10B098091FB51" modified="2020-10-07" version="1">MEKLCVVMEVTPMDSSRSMTYVEKDSLVQRLARGLHKINAQTLEAGITDRTPATKTIAFLQKQIFEFTQRLHTAEVERRSLRLELTEFKRNLNEMKKEADKAHSFQEQLYVFKQSKLITHERFESACEELNNALLREQQAQMLLKEQAQQLQELQFKLELHSSEEADKKQTLSDAVKSLSEAKMELRRKDQSLRQLNRHLTQLEQDKRRLEESIHDAESALRMAAKDKELIVSHMKSVESIFQNVRDQISLSWTTASRNDFALQLPKLHLETFAMEGLKGGPEVIAFQAMIISFIDVYQFACSRVSALEREITSHRHHIATLKSELQTACLRENESLHSDSRNASSILTVSSSLPQLDQGCLPGFLPLKSESDYDLPFTLAHNRSPISSSFPSNLHVMSAKRAMPNGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMM7</accession>
    <name>A0A6I9YMM7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Transmembrane protein 52B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">TMEM52B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925244.1">
        <property type="nucleotide sequence ID" value="XM_014069769.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925245.1">
        <property type="nucleotide sequence ID" value="XM_014069770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMM7"/>
    <dbReference type="GeneID" id="106551628"/>
    <dbReference type="KEGG" id="tsr:106551628"/>
    <dbReference type="CTD" id="120939"/>
    <dbReference type="OrthoDB" id="9361294at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038942">
        <property type="entry name" value="TMEM52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33955">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33955:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 52B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14979">
        <property type="entry name" value="TMEM52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636651" evidence="2">
        <location>
            <begin position="26"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013925244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925245.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="20663" checksum="40C40D63510625C8" modified="2020-10-07" version="1">MMSKGAQVVAVSILACLAQIPQVKLQENCTNNDPCSHTNWIHHWYIWLVVGIGGLLLLCALVSVCLRCCCFNCHSGEEDAGPQPYEVTVIAFDQDTTLQSTITSFHSMFGPAARRILAVAHSHSGAPAVHHPARTEPPPPIYEEAIHVNRFTVARSGERVVDLELMPEEKQIIGPKKEEEQEAASTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YND4</accession>
    <name>A0A6I9YND4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein ITFG3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FAM234A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FAM234 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925777.1">
        <property type="nucleotide sequence ID" value="XM_014070302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YND4"/>
    <dbReference type="GeneID" id="106552113"/>
    <dbReference type="KEGG" id="tsr:106552113"/>
    <dbReference type="CTD" id="83986"/>
    <dbReference type="OrthoDB" id="6364780at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055409">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045232">
        <property type="entry name" value="FAM234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419:SF7">
        <property type="entry name" value="PROTEIN FAM234A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23727">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="FAM234A/B beta-propeller" evidence="8">
        <location>
            <begin position="81"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013925777.1"/>
        </source>
    </evidence>
    <sequence length="557" mass="60465" checksum="C1A6E35952F8CF15" modified="2020-10-07" version="1">MAGTETEAEFHPLKAREAPATPRGSGARDAEKEAGDGAQPRRLSKWRAAAFFLSVYLCLGVAFAFSFVIPCPVRPVSRTTWSRAYDDAAAYAFLAAGDADGDRVQDLLVAFLAGPGSRNDSGADEGSCFGAGFATPCAFLAAHSGTNGTTLWKRPVAQELLLTDCSVEHGGSPACLIVGNPASLALLDLETGQTRWNGSVDFGTNSTVLSPLLKIPDIDKDGVPDFLVFAATGEKIKSFFYSGTFGTQMKFHGTLHLPGLIGHLLHVTKSGAHYILFYTAKAVFAYSLKELYRTAVGPAGPAPATLKEDAGWEKAIAHKAREVPLASSGEIQYVTKMSEKPGSHILVARSAMLELVNGQRLSSLWVVGVPHILREPVLGAFGPDEIAVAIESRVSHDTKKVTIVEGSSGTIKWEVELLSEMQSPKPATLATVDRRSLFLFWGLYQDERNGTGSWHGSRQQRLYLFHPSLPTVLLELSNSADPIVAFEGLLFERSRHACYVLLAGPQVGSSPGRVVLSKRKLKEDIFSSHVIWLNQMPQDTDQNVRDYFHRMRFRTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNK8</accession>
    <name>A0A6I9YNK8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 408</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZNF408</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926068.1">
        <property type="nucleotide sequence ID" value="XM_014070593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNK8"/>
    <dbReference type="GeneID" id="106552340"/>
    <dbReference type="KEGG" id="tsr:106552340"/>
    <dbReference type="CTD" id="79797"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002169">
        <property type="entry name" value="Zgc:174573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003336">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000849">
        <property type="entry name" value="Zinc finger protein 408"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001101">
        <property type="entry name" value="Zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001136">
        <property type="entry name" value="Zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF23">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="434"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="468"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="496"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="579"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="308"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="317"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013926068.1"/>
        </source>
    </evidence>
    <sequence length="732" mass="82034" checksum="2B70FED4F7636BB8" modified="2020-10-07" version="1">MHGKFPFCPAWHLPRGLALGPSLSSEKGLGVWCVGHALEPGMSLLLGPQVEEQKAAASLSACPLASWKEASACNGISSWRSLVKQSRVEDEANVILEWINGELHIKVCCPVDVGSELLCWLTESQDSPTCLKNNILAHTSDGELDTEKKTLQKMPAITTTKTRDLEDETLLQKDDIVQDSSAGIAGMSKFAELKDRACCVSVLNKKLQMEQILGLDVNLKKNSRTSSLQIEQLNTKIKSRNNKLLNKYPVSKDDEGKEDSQEQQMAEKKLATTLTEICSNPTPRSVVCISAILTDNVEPLRKESCAERIPNGYLPSRPKDRAENPRQEEQSHRYKPDDSAHTESSTKRQHRCKECGKAFLQLCHLKKHHFVHTGHKPFLCTECGKNYSSEESFRAHVLGHSGVRPFPCPQCHKTYGTKRDLQEHQVLHTGQRPYVCPDCGKSFARRPSLRIHRKTHQVKQPSSITSTYQCVICGRHLANPGSLRNHMRLHTGERPYTCPYCPKSFGQQGNLRGHLRLHTGERPFHCHFCGDAFPQRPELLRHLISHTGEAYLCTVCGKALRDPHTLRAHERLHTGERPFRCHLCPKSYPLATKLRRHQKAHHGDKPHQYNVCGIDYSLPQKLQRHKLEDKDNIVNDSSKSDIKYLKDTAISEQPAVHALSVAEPGRKAEVFISNTKYISAQDGSAQHKDVIEVAEEECIIVQEQASPSNMFILQEGVGFSAVAEVIEVELGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPA8</accession>
    <name>A0A6I9YPA8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GATOR complex protein NPRL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Nitrogen permease regulator 3-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NPRL3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NPR3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925739.1">
        <property type="nucleotide sequence ID" value="XM_014070264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPA8"/>
    <dbReference type="GeneID" id="106552070"/>
    <dbReference type="KEGG" id="tsr:106552070"/>
    <dbReference type="CTD" id="8131"/>
    <dbReference type="OrthoDB" id="18648at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056603">
        <property type="entry name" value="HTH_NPRL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005365">
        <property type="entry name" value="Npr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153">
        <property type="entry name" value="CGTHBA PROTEIN -14 GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153:SF5">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24064">
        <property type="entry name" value="HTH_NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03666">
        <property type="entry name" value="NPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GATOR1 complex protein NPRL3 C-terminal HTH" evidence="4">
        <location>
            <begin position="513"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="455"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="460"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013925739.1"/>
        </source>
    </evidence>
    <sequence length="591" mass="66109" checksum="0C96D87240CEC9F5" modified="2020-10-07" version="1">MGDTSSPVSVILVSSGSRGNKLLFRFPFQRGPGNPAALIGTSRSRYAVNSIGDNVEDPDGDSREPCPLTDEQVVSGFSDVILATILATKSDMCGKKFELKIDNIRFVGHPTLLQHAFGQVSKTDPSPKREMPTMILFNVVFALRATADPSVISCLHNLSRRIAIVLQHEERRCQYLTREAKLILAIQDEVSAMSETREGPQSPFPHILPKCKLARDLKEAYDSLCTTGLVQLQINNWLEVSFCLPHKIHDVASSFIPPEAIERSLKAIRPYHALLLLRDEKSLLSELPLDCSPALVRVIKTASAVKNLQQLAQDADLALLQVFQLAAHLVYWGKALMVYPLCENNVYMLSPNANVCLYSPLAEEFSSQFPGQDLPAVLSKFSWPVSLSELKDPLAPAIQETHLIRMVAWMLQHRLLIQLHSYACLMVPPKEEEEEEEEEEEGFRTRVEELSFPARVGGRSLSTPSALSFGSPTSSDDMTLTSPSMDNSSAELLPGGESPVNKRMTENLLTSLSEHEREAILSVPAAHNPDDLRMFARLLHYFRGRHHLEEIMYNENMRRSHLLMLFDKFRSVLVVTNHEDPVISVFQSLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQC1</accession>
    <name>A0A6I9YQC1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106552681</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926487.1">
        <property type="nucleotide sequence ID" value="XM_014071012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQC1"/>
    <dbReference type="GeneID" id="106552681"/>
    <dbReference type="KEGG" id="tsr:106552681"/>
    <dbReference type="OrthoDB" id="435430at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903527">
        <property type="term" value="P:positive regulation of membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08848">
        <property type="entry name" value="ArfGap_ASAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13251">
        <property type="entry name" value="PH_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000002">
        <property type="entry name" value="arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000004">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000001">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000006">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF2">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="307"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="13">
        <location>
            <begin position="422"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="694"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="762"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="706"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="718"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="762"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="793"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926487.1"/>
        </source>
    </evidence>
    <sequence length="855" mass="95599" checksum="8066A4E76B21AE60" modified="2020-10-07" version="1">MPDQITVSEFIAETTEDYNSPTTSSFTTRLQNCRNTITLLEEALDQDRTALQKVKKSVKAIYNSGQEHSQNEESYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGIKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSRQGGYSMHQLQGNKEYGSEKKGYILKKSDGIRKVWQRRKCTVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLITHNRTYHFQAEDEQDYVAWISVLTNSKEEALNMAFRGEQSTGENSLEDLTKAIIDDVQRLSGNDVCCDCGSPDPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNIGNNSFNDIMEGNLPTPSPKPTPSSDMTARKEFITAKYVDHKYSRKTCASAAAKLNELLEAVRSRDLLALLQVYAEGVELMEPLLEPGQEPGETALHFAVRTADHTTLHLIDFLVQNCGNLDKQTVLGNTALHYCSIYNKPECLKLVLKGKPTLHIVNQAGETALDTAKRQKAIQCEDLLSQAKVGKLNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKMAIPGLSTPREKQRLSYGAFPHQIFVSGSIDSATSPTTVDGPPLPPRNAGKGPTCPPSTLPLSTPASSGSSTLSKKKPPPPPPGHKRTLSDPPSPQPHGPQNKVQMSWGNDLGPPATKTANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQW6</accession>
    <name>A0A6I9YQW6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Helicase SRCAP-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106552839</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926692.1">
        <property type="nucleotide sequence ID" value="XM_014071217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQW6"/>
    <dbReference type="GeneID" id="106552839"/>
    <dbReference type="KEGG" id="tsr:106552839"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF1">
        <property type="entry name" value="HELICASE SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="257"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="179"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926692.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33852" checksum="51DE09CF4643DB15" modified="2020-10-07" version="1">MERFNADKRIFCFILSTRSGGVGVNLTGADTVVFYDSDWNPTMDAQAQDRCHRIGQTRDVHIYRLISERTVEENILKKANQKRMLGDMAIEGGNFTTAYFKQQTIRELFDMPLDEPSKKEGEAPIPVQEEEEDPTATKQTQILEQALCKAEDPEDIWAATQAKAEQVAELAEFNENIPLDAEDRSSKEEEEEFSKAEQEIASLVEQLTPIERYAMNFLEASLEDISREELKQAEEQVEAARKDIDQAKDEVVFKLPEDEDESYLLEEGSSKKSKKAKMSSRTFSERTGTRMSERLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS27</accession>
    <name>A0A6I9YS27_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tyrosine-protein phosphatase non-receptor type</fullName>
            <ecNumber evidence="10">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 21 22 23 24 25 26" type="primary">PTPN6</name>
    </gene>
    <organism evidence="19 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013926432.1">
        <property type="nucleotide sequence ID" value="XM_014070957.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926508.1">
        <property type="nucleotide sequence ID" value="XM_014071033.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926584.1">
        <property type="nucleotide sequence ID" value="XM_014071109.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926660.1">
        <property type="nucleotide sequence ID" value="XM_014071185.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926739.1">
        <property type="nucleotide sequence ID" value="XM_014071264.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926816.1">
        <property type="nucleotide sequence ID" value="XM_014071341.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926887.1">
        <property type="nucleotide sequence ID" value="XM_014071412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS27"/>
    <dbReference type="GeneID" id="106552603"/>
    <dbReference type="KEGG" id="tsr:106552603"/>
    <dbReference type="CTD" id="5777"/>
    <dbReference type="OrthoDB" id="8815311at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09931">
        <property type="entry name" value="SH2_C-SH2_SHP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10340">
        <property type="entry name" value="SH2_N-SH2_SHP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000012">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000018">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000018">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052123">
        <property type="entry name" value="Non-rcpt_Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012152">
        <property type="entry name" value="Tyr_Pase_non-rcpt_typ-6/11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46257">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE CORKSCREW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46257:SF4">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000929">
        <property type="entry name" value="Tyr-Ptase_nr_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 13" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="3"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="109"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="17">
        <location>
            <begin position="250"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="18">
        <location>
            <begin position="436"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="555"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
        <location>
            <position position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="506"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013926432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013926508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013926584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013926660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013926739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013926816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013926887.1"/>
        </source>
    </evidence>
    <sequence length="607" mass="69343" checksum="D027BACEA2204979" modified="2020-10-07" version="1">MRWFHRDLSGIDAEALLKARGIHGSFLARPSRKNKGDFSLSVRVGDEVTHIRIQNTGDYYDLYGGEKFATLSELVEYYTQQQGSLQDKDGTIIHLRYPLNCSDPTTERWYHGHLSGPAAEALLQAKTAPWTFLVRESLSKPGDFVLSVLTDQPKPRTDATGAHSQERLKVTHIKIMCEKGQYTIGGTARFVSLADLVEHFKKTGIEEASGYYVYLRQPFNATRVNAADIENRVEMLKRRSQAEEANKGGFWEEFDSLQKQETKNLYDRHEGQRPENKSKNRYKNILPFDHSRVILQDRDVNIPGSDYINANYIKNELFGPEEYSKTYIASQGCLEATINDFWQMVWQENSRIIVMTTREVEKGRNKCVPYWPEVGSSKEYGPYIVENIGDREAIDYKLRQLRLSPINNGEAVRDIWHYQYLSWPDHGVPSEPGGVLNFLDELNRMQESAPGAGPIIVHCSAGIGRTGTIIVIDMTVDMISTKGLDCDIDIPKAIQMVRSQRSGMVQTEAQYKFIYMAICQFIEITKKKRDILQSPKGRLNESEYGNITYPLAQKISHPKISRKSSRQKEEPTLYENLDNMKGKKEEKGHKPWPSGKEKKPKGSLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTG7</accession>
    <name>A0A6I9YTG7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amyloid protein-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106553395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927351.1">
        <property type="nucleotide sequence ID" value="XM_014071876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTG7"/>
    <dbReference type="GeneID" id="106553395"/>
    <dbReference type="KEGG" id="tsr:106553395"/>
    <dbReference type="OrthoDB" id="7103806at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042476">
        <property type="entry name" value="APPBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46575">
        <property type="entry name" value="AMYLOID PROTEIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46575:SF1">
        <property type="entry name" value="AMYLOID PROTEIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013927351.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="12963" checksum="F5B96A7A06EED9E9" modified="2020-10-07" version="1">MAAVELEWVPETLYNTAISAVVDSYGRARRRDIRSLPENIQFDVYYKLYQQGRLCQLGSEFCELEVFAKVLRALDKRHLLHHCFQALMDHGVKVASVLAYSFSRRCSYIAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTR4</accession>
    <name>A0A6I9YTR4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamate-rich protein 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ERICH6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927953.1">
        <property type="nucleotide sequence ID" value="XM_014072478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTR4"/>
    <dbReference type="GeneID" id="106553895"/>
    <dbReference type="KEGG" id="tsr:106553895"/>
    <dbReference type="CTD" id="131831"/>
    <dbReference type="OrthoDB" id="527209at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029281">
        <property type="entry name" value="FAM194_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23093:SF18">
        <property type="entry name" value="GLUTAMATE RICH 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23093">
        <property type="entry name" value="SIMILAR TO CHROMOSOME 3 OPEN READING FRAME 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14977">
        <property type="entry name" value="FAM194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM194 C-terminal" evidence="2">
        <location>
            <begin position="378"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927953.1"/>
        </source>
    </evidence>
    <sequence length="619" mass="70027" checksum="16470FA0D1934FCD" modified="2020-10-07" version="1">MMEGFSQTPRKNRDGGSDSDSKDAGEGSSSSEADNSDKSSKEIVIGEINGSPDMRSDRGSSEIKNELPSPSATETSTSSTGKTKSFPSILGGVRRESEIRREILDTIADMPERPLEISVSMQTDASWLHDHIYKRKSPLSAPKEHQEEVPPENDIYAVVPQDLSYLEILCDTEFREDFLKLFQESLQTISSVGPPTIIAYRPESSRADIHIEVPKETLETCEFCGSPIKYFPSFESLEHIAEWGNRFCCQQCRELHEFIVNEKNRYPVKMEKINIAPHKAHGSQADRERAKERARQRLKERQIAKQVAYMAVEAEKSALPMHIPEYNKQLKTISFLMSQETLSSAIIHPPKIKKAEVVDNTYSISCCDLTIANGKLIKNQFIQQYYKNGVKFLTMFPDGTAQIFYPSGNLAIIIVQNKNAGYICIVQEDKREKPKIQAIFDSSGKGTCYHPNQIVWINITIRGGHYSDQVGNKVKTWIWPNNLQKHNPRVVFKPIFLSLNLNVGVRILGQDKIAISFLAMGKQAKINVGTKVQPLAEHLPWPKCVAEDDLLLFAKRLKILRIFAKLNRYLDFPASDWWTKLQLPSFLLKQTSKLIYLCKECEIGCALDKIIMAILNSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTV0</accession>
    <name>A0A6I9YTV0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KH domain-containing, RNA-binding, signal transduction-associated protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106553705</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927737.1">
        <property type="nucleotide sequence ID" value="XM_014072262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTV0"/>
    <dbReference type="GeneID" id="106553705"/>
    <dbReference type="KEGG" id="tsr:106553705"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22470">
        <property type="entry name" value="KH-I_KHDRBS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000123">
        <property type="entry name" value="KH domain-containing, RNA-binding, signal transduction-associated protein 3 isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF29">
        <property type="entry name" value="KH DOMAIN-CONTAINING, RNA-BINDING, SIGNAL TRANSDUCTION-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927737.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="19371" checksum="8C1BCE0D515AD7B4" modified="2020-10-07" version="1">MKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFLIPDYNDEIRQAQLQELTYLNGGSENAEVPVVRGKPPLRARGGLSPALARYAGDVCVCVCLCGVKRSNSGCWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUP5</accession>
    <name>A0A6I9YUP5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vomeronasal type-2 receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106553406</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927365.1">
        <property type="nucleotide sequence ID" value="XM_014071890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUP5"/>
    <dbReference type="GeneID" id="106553406"/>
    <dbReference type="KEGG" id="tsr:106553406"/>
    <dbReference type="OrthoDB" id="9044658at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000024">
        <property type="entry name" value="Vomeronasal 2, receptor 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="11">
        <location>
            <begin position="269"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927365.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="49481" checksum="81F93DF7DD1733B7" modified="2020-10-07" version="1">MTASFYRMAPNEDLQITGIIHLLKHFGWTWVGLLIMDDDSGDNFLQALDPLLSENRICSAFVERMPKQVFFTVFQNSVVYKVNNYYFYSKINTFIFYGDSTTLMLLLMFLKYDSLWARVPKIWILSAQMDLMLTSFHRPSDLQLFDGAISFDIHSNELPRFGEFLGAIKPFQTKRDSLVKEFWEQAFDCILSENNPPGESGEACTGKEDLKNLPPHLFEVTGHSYSIYNAIYAVAHAVDDMNLLRSKYKRATGSRRVELQDQDIWKRLVSFKDVAVYFTEGQGALLDPAQRALYKEVMVENYENMASLGFPVPKPNLITRLEQGEPPWRLDFQGVDANNSGALGKKNSCRKCGKCFVQKSDLAVHRLVHKGEKTFKCLECSKYFVHRSELVKHQRVHTGERPFKCFECGKCFSRQSVLSNHHRIHTGEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV86</accession>
    <name>A0A6I9YV86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prostaglandin E synthase 2</fullName>
            <ecNumber evidence="3">5.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Microsomal prostaglandin E synthase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PTGES2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>prostaglandin H2 = (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoate + malonaldehyde</text>
            <dbReference type="Rhea" id="RHEA:48644"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:90694"/>
            <dbReference type="ChEBI" id="CHEBI:566274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:48645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>prostaglandin H2 = prostaglandin E2</text>
            <dbReference type="Rhea" id="RHEA:12893"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="5.3.99.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:12894"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; prostaglandin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Endomembrane system</location>
            <topology evidence="18">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.99.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013928212.1">
        <property type="nucleotide sequence ID" value="XM_014072737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV86"/>
    <dbReference type="GeneID" id="106554132"/>
    <dbReference type="KEGG" id="tsr:106554132"/>
    <dbReference type="CTD" id="80142"/>
    <dbReference type="OrthoDB" id="423541at2759"/>
    <dbReference type="UniPathway" id="UPA00662"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050220">
        <property type="term" value="F:prostaglandin-E synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03197">
        <property type="entry name" value="GST_C_mPGES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03040">
        <property type="entry name" value="GST_N_mPGES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000028">
        <property type="entry name" value="Prostaglandin E synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000114">
        <property type="entry name" value="Prostaglandin E synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034334">
        <property type="entry name" value="PGES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034335">
        <property type="entry name" value="PGES2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12782">
        <property type="entry name" value="MICROSOMAL PROSTAGLANDIN E SYNTHASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12782:SF5">
        <property type="entry name" value="PROSTAGLANDIN E SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01182">
        <property type="entry name" value="Prostaglandin_E_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01203">
        <property type="entry name" value="Prostaglandin_E_synthase_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GST C-terminal" evidence="19">
        <location>
            <begin position="250"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013928212.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="42049" checksum="6F781AC38B002415" modified="2020-10-07" version="1">MALARLACNGWAGLGRSAWRRLPRAGFSSSFSGRPGRGWSRPSSRLWLGAAFALGAAVGTGRTLHRRFGAQEQLAEQHRDGILPDSHLHLTLYQYKTCPFCSKVRAFLDYHRLPYDIVEVNPVMRKEIKFSSYRKVPILLIDTESTLQLNDSSVIISTIKSYLISRKSLEEIASYYPSIKAINSEGKEVAEFGNKYWLMLDSKEALCVYPVEEARKEEMKWRKWVDDRLVHLISPNVYRTPGEALASFDYIVREGKFGLVEGFFAKYVGAAAMFFVSKRLKKRHHMRDDVREDLYEAVNDWMKAVGKHRKFMGGDHPNLADLAVYGVLRVMEGLEAFGDVMEHTKIQPWYQRVEDAIGQGEAARWARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVT7</accession>
    <name>A0A6I9YVT7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">T-cell immunomodulatory protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928397.1">
        <property type="nucleotide sequence ID" value="XM_014072922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVT7"/>
    <dbReference type="GeneID" id="106554278"/>
    <dbReference type="KEGG" id="tsr:106554278"/>
    <dbReference type="OrthoDB" id="10250728at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024881">
        <property type="entry name" value="Tip"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412:SF0">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13517">
        <property type="entry name" value="FG-GAP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026853429" evidence="2">
        <location>
            <begin position="38"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928397.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="20099" checksum="BCCA5E6C3734F1FD" modified="2020-10-07" version="1">MGAPCRPVLARRGRSSLLWPPLLLLVTVGALLKPVRALQNVTSQLFGAEAFGTLAAFGDFNSDKQTDLFVLRGENELNIFLADQNEPYFKPKVKLLLRDVVITSVVPGDYDGDSQMDALLTTRPQEQPDADLSIWIFWGNNQTLGRLYRRKYSLSSSTKQLRISLFSGECERQMYLNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWA7</accession>
    <name>A0A6I9YWA7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ephexin-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NGEF</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928582.1">
        <property type="nucleotide sequence ID" value="XM_014073107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWA7"/>
    <dbReference type="GeneID" id="106554446"/>
    <dbReference type="KEGG" id="tsr:106554446"/>
    <dbReference type="CTD" id="25791"/>
    <dbReference type="OrthoDB" id="27593at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01221">
        <property type="entry name" value="PH_ephexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11939">
        <property type="entry name" value="SH3_ephexin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000127">
        <property type="entry name" value="Neuronal guanine nucleotide exchange factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000007">
        <property type="entry name" value="rho guanine nucleotide exchange factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035635">
        <property type="entry name" value="Ephexin-1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047271">
        <property type="entry name" value="Ephexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047270">
        <property type="entry name" value="PH_ephexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845:SF8">
        <property type="entry name" value="EPHEXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="DH" evidence="8">
        <location>
            <begin position="352"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="568"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="691"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="205"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="278"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013928582.1"/>
        </source>
    </evidence>
    <sequence length="783" mass="90169" checksum="1E56EE61D1CEB424" modified="2020-10-07" version="1">MEKENLEEVQRHHRGRSANGIQKDSPPQAISSLDLISDDESEPSDSITEKEPKTHYIPIRRNSIYYRSIRRRNKKKPRSSKEVQTEHFEGSLKNGNTKNELLNKEELLHIFHKSLSLHVKPNMTATEEQWFQRLLPSYPGYRGNRPDFPGPASVAESLGSSSSSSRRRRPLPESLLLPLSSSPPGMELLAAALSAACALDHEGSGENVAAGRSADSESPSMAGPTSFPEETPEQTECPAAFPEALQMIHPMTADSWKNFIEQIGLLYQEYRDKSTRHEIETRRLQDSQTDTEEGSTTEESPTETETENVLENKTVPQMNLLRNSTSRFNLWQDLPEIRSSGVLNILQPDEVKLQEAMFELVTSEASYYKSLNLLICHFMENDRLKKILHPSEAHILFSNVLDVMAVSERFLLDLEKRVEENIVISDVCDIVHQHTVSYFSVYVTYVSNQTYQERAYKQLLQDKPAFREVILQLELDPKCKGLPFSSFLILPFQRITRLKLLVQNILKKVEEKSEREITALEAHKELEIIVKACNEGVRKMSRTEQMISIQKKLEFKIKSVPIISHSRWLLKQGELQQMNGPKTSRTLRTKKLFREIYLFLFNDLLVLCRQINGDKYQVFDSASRGLLRVEELEDQGQSLANVFILRLLENADDREVSYMLKALSQSEMKRWMILLAPNRRTKFVSFTSRLMDCPQVQCVHPYVAQQPDELSLELADVLNILEKTDDGWIFGERLHDQERGWFPSSMGEEIMNPKIRSQNLKECFRVHKTDDSQRRKMGSRNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX07</accession>
    <name>A0A6I9YX07_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Cell division control protein 42 homolog</fullName>
            <ecNumber evidence="13">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106554373</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013928496.1">
        <property type="nucleotide sequence ID" value="XM_014073021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX07"/>
    <dbReference type="GeneID" id="106554373"/>
    <dbReference type="KEGG" id="tsr:106554373"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051130">
        <property type="term" value="P:positive regulation of cellular component organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01874">
        <property type="entry name" value="Cdc42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000167">
        <property type="entry name" value="Cell division control protein 42 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037874">
        <property type="entry name" value="Cdc42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="15" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013928496.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="21744" checksum="F2111668846CFDC4" modified="2020-10-07" version="1">MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPEAAEKLARDLKAVKYVECSALTQVSPTHPCLNQCPRLLLAEGEVWGGGSSESNGVRSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX95</accession>
    <name>A0A6I9YX95_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interferon-inducible GTPase 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106555038</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929278.1">
        <property type="nucleotide sequence ID" value="XM_014073803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX95"/>
    <dbReference type="GeneID" id="106555038"/>
    <dbReference type="KEGG" id="tsr:106555038"/>
    <dbReference type="OrthoDB" id="422720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF16">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="92"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929278.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="48086" checksum="0BE0E1C8EEB0F21A" modified="2020-10-07" version="1">RKELSEKLTKDGLEAKIFLVSGLQTERYDFASFEEALEGEVLNLKRHHEGNLKDFHAVSQKTVKELFEICKFSSLADVPGVIQAMLANPTQIHLNVALIGETGSGNSSLINALRRAGAEEAGAAPTGIAETTRVATAYPFPSVPNMFLWDLPGVGLMEDDVKRLDLSRYSAFLLVASERYKHIHSCLAKMIASEGKQSFFVRNKIDVDVEAPGGSQPNVKEKLQEQIRKSCVEALKNDGVECPVFLVSSFMAEAYDLPLLREELQKRAAELKRKALRRAIPTVVSQLVRLKSKVLMKDVWGKTLQVGLSSVDGFKETEVKKLLAIIASFCIHLGLNETSVARTAQCTGKEIHWLHEQIQSRFACSMPPTEVLNLIVKPPSWGNWAWSYMPYWGRGGKVEATISVENVYNLLKQAVVELSEDAERLLLAAFSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXB1</accession>
    <name>A0A6I9YXB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">RET</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013929293.1">
        <property type="nucleotide sequence ID" value="XM_014073818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXB1"/>
    <dbReference type="GeneID" id="106555050"/>
    <dbReference type="KEGG" id="tsr:106555050"/>
    <dbReference type="CTD" id="5979"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000190">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase receptor Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000234">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase receptor Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041317">
        <property type="entry name" value="RET_CLD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055162">
        <property type="entry name" value="RET_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF485">
        <property type="entry name" value="PROTO-ONCOGENE TYROSINE-PROTEIN KINASE RECEPTOR RET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17813">
        <property type="entry name" value="RET_CLD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22540">
        <property type="entry name" value="RET_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="293"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013929293.1"/>
        </source>
    </evidence>
    <sequence length="549" mass="61488" checksum="34B52DDB018FCE1C" modified="2020-10-07" version="1">MKFYGVSITYSLESPNVSCYAVEVAPSHDDKYGILYVNNSALLQSPECRVIHYPVLAKEEWSRKEAFAHLTVILEGSLIQNDKNCPDSCAMSKRRPECEECGGFGVLMGKCQWRQGSGKGITTNYSTCTPSIKTCPDGICDAVESKDKRICPQDCTRTNIIGGHLKGSLGTGIKAGHGVCHCFPGISCYCAKDDIRDPFCDDMCKMVIAGGVLLSFIISVVLSSYFIHRYHKNAPKTPIASAEMTFRRPAQAYPINYSSNNVRRPSMDSENQMSVDAFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASSSELRDLHSEFNLLKQVNHPHVIRLYGACSQDGPLYLIVEYAKYGSLRSFLRESRKVGTSYMGSDTNRNSSYLDNPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRDVYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIAPERLFNLLKTGYRMEKPENCSEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXF1</accession>
    <name>A0A6I9YXF1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UPF0729 protein C18orf32 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0729 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928977.1">
        <property type="nucleotide sequence ID" value="XM_014073502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXF1"/>
    <dbReference type="GeneID" id="106554769"/>
    <dbReference type="KEGG" id="tsr:106554769"/>
    <dbReference type="OrthoDB" id="10062823at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026776">
        <property type="entry name" value="UPF0729_C18orf32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13456">
        <property type="entry name" value="UPF0729 PROTEIN C18ORF32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13456:SF0">
        <property type="entry name" value="UPF0729 PROTEIN C18ORF32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14975">
        <property type="entry name" value="DUF4512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928977.1"/>
        </source>
    </evidence>
    <sequence length="97" mass="11120" checksum="BB556166CA9A90E9" modified="2020-10-07" version="1">MVCIPCIVIPVLLWVYKRFLEPYLYPFISPFVKRIWPKKAVQETAKQDQKNSSENGHDLVKQDSFNDQPELYKTKSNGIANGFSGIGPKEDSDKKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1D2</accession>
    <name>A0A6I9Z1D2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glycerol-3-phosphate phosphatase</fullName>
            <ecNumber evidence="13">3.1.3.21</ecNumber>
            <ecNumber evidence="5">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Aspartate-based ubiquitous Mg(2+)-dependent phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Phosphoglycolate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">PGP</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear. In vitro, also has a phosphatase activity toward ADP, ATP, GDP and GTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>sn-glycerol 1-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:46084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>sn-glycerol 3-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:66372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="24">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.21" evidence="13"/>
    <dbReference type="EC" id="3.1.3.48" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013930402.1">
        <property type="nucleotide sequence ID" value="XM_014074927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1D2"/>
    <dbReference type="GeneID" id="106555976"/>
    <dbReference type="KEGG" id="tsr:106555976"/>
    <dbReference type="CTD" id="283871"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043136">
        <property type="term" value="F:sn-glycerol 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000163">
        <property type="entry name" value="Pyridoxal phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF92">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 24" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="22">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="22">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00057228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013930402.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="33280" checksum="22D9D776E2D635ED" modified="2020-10-07" version="1">MAVKECRRLDAALARQLLSQTEALLFDCDGVLWRGEAVLLGAPETLHRLELGGGKRLCYVTNNSSRTRQAYTEKLLRLGFPPAEPGQVFGSAYCAARHLSRALPPGGTAYVLGGPALSAELEALGVAHLGVGPAPEPGSAHFGARAPLDPSVCAVLVGYDEHFTYGKLCLALRYLLRGDGDGRGCLLVGTNRDNRLPLEGGSAVPGTGCLVKAVETAAEREAFIVGKPSRFMFECVANEFKINPARTIMVGDRLDTDILMGNTCGLTTLLTLTGVTTLDEVKGHLNSGCPERRSLVPDYYVNSIADLLPALTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z219</accession>
    <name>A0A6I9Z219_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Laminin subunit alpha-5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106556204</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930667.1">
        <property type="nucleotide sequence ID" value="XM_014075192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z219"/>
    <dbReference type="GeneID" id="106556204"/>
    <dbReference type="KEGG" id="tsr:106556204"/>
    <dbReference type="OrthoDB" id="18487at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000022">
        <property type="entry name" value="Laminin subunit alpha 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF406">
        <property type="entry name" value="LAMININ SUBUNIT ALPHA 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026872754" evidence="3">
        <location>
            <begin position="33"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="4">
        <location>
            <begin position="42"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930667.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="43293" checksum="5F149B10D1CB2403" modified="2020-10-07" version="1">MASRPVPAAGLRCPLALLVVVVAAALPGASWAQPVSFQADVNGYSLHPPYFNLAEGTQISATATCGEEEAGGRSPPRPVEDLYCKLVGGPVSGGEPNQTIQGQYCDICSSSNSNKAHPITNAIDGTERWWQSPPLSRRLEFNEVNVTLDLGQLFHVAYVLIKFANSPRPDLWVLERSTDFGETYDPWQYFASSRRDCIEKFGLKSREKVTKDDDVICTTEYSRIVPLENGEIVVSLVNGRPGAMNFSYSPLLRNFTKATNIRLRFLRTNTLLGHLMGKALKDPTVTRRYYYSIKDISIGGRCVCHGHADVCDAKDPKDPYRLQCDCQHNTCGGSCDHCCPGFNQLPWKPATTDSANECEPCNCNGHAYDCYYDPDIEHQKASKNRYDQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z238</accession>
    <name>A0A6I9Z238_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CWF19-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106556228</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWF19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930692.1">
        <property type="nucleotide sequence ID" value="XM_014075217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z238"/>
    <dbReference type="GeneID" id="106556228"/>
    <dbReference type="KEGG" id="tsr:106556228"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF5">
        <property type="entry name" value="CWF19-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="470"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930692.1"/>
        </source>
    </evidence>
    <sequence length="565" mass="65565" checksum="D3670CEBF961440C" modified="2020-10-07" version="1">MASGFESERVLAARKEDRRRQREALLQQAKANYEKDERRKELKRLRGEDTWMLNDVNERLEEMKKEHAVTKKHSKKHKRKKKKKKITREKTDDSSDSSSGSSEEWVEASSSTEQAWKAKKEQSEEETVENPTPIQRDEWMTLDFMTVKTLSSASLKAEKQKEKILKQQKAQENEQLLLSERELNPYWKDGGTGLPTEVSSSVKATTVEDGGLSWLMKSYQRMQEQSEREKRDLEEIVAERYGSVELFQSKLREAETAAVQKENDRRDWQRYKYSESKRTSRDCKEKQEENNLEKFSHKEQFKEKYNKVRCEEPKNYRESEWTETKKSRPSNKESTAANWTCTTRKDPHQLSHYEKPFSSSMKAAFLKPSSDADEGSRQQRTSSQLLTPRFMSSGFRKPVDDAGVTPRWRKAQPKDEIGKATPNEKSSETSKAVTSKEEEEPKEKEEQISEPAVEEKQLVDSKPSCSRSVSEDETPLILSDEEMNKLGARIVKAELMGNKDLAAKLQSQLDKARMLKESGAHAKARKTDEANKLRTKTLRNTLNPTWNETLTYYGITDEDMIRKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2F8</accession>
    <name>A0A6I9Z2F8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">DDX27</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013930055.1">
        <property type="nucleotide sequence ID" value="XM_014074580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2F8"/>
    <dbReference type="GeneID" id="106555685"/>
    <dbReference type="KEGG" id="tsr:106555685"/>
    <dbReference type="CTD" id="55661"/>
    <dbReference type="OrthoDB" id="10259843at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17947">
        <property type="entry name" value="DEADc_DDX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000842">
        <property type="entry name" value="ATP-dependent RNA helicase DRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF22">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="200"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="385"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="72"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="663"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
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        <location>
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            <end position="676"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="699"/>
            <end position="711"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="723"/>
            <end position="735"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="736"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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    <evidence type="ECO:0000256" key="8">
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        <source>
            <dbReference type="ARBA" id="ARBA00043999"/>
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            <dbReference type="ARBA" id="ARBA00047984"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013930055.1"/>
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    <sequence length="749" mass="84016" checksum="A30D9EFB5B2281C6" modified="2020-10-07" version="1">MEAGAGAKRSLEEPDAEPEQTDSESEEEPAAAGRGDFAAGFAFSEKEAAAGAGSGAWAEALGQIKRKRCATSLDEKIATVRRQRRAQEKATTDGEAKREGGETTQENEAKAGDSEEEEELRSEHSSADESVFAKADTLKLKQRWKQKHGEKEEATQFFQEDASQYDDSLSFQDMNLSRPLLKAITALGFKQPTPIQKACIPVGLLGKDLCACAATGTGKTAAFILPVLERLIYKPRQAPVSRVLVLVPTRELGIQVHSVTKQLAQFSSITVCLAVGGLDLKTQEAALRCGPDILIATPGRLIDHLHNCPSFHLGSIEVLILDEADRMLDEYFEEQMKEIIRMCARHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTEVAPFLRQEFIRIRPSREGDREAIVAALLTRTFPDHVMLFTQTKKQAHRLHILLGLMGLRVGELHGNLSQAQRLEALRQFKDEQIDVLVATDVAARGLDIEGVKTVINFTMPNTTKHYVHRVGRTARAGRVGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILRFREKIEKLEPDVYAVLRLEREERELQCSEAQINTAKRKLDSGSQQAAGGPPRSWFQSQEERKKEKLAKALQEFDLALRGKKKRTKFVRDSQKKVQPTPEERSQFEILKAQMYAERLSKRSRRPKRARAMPEETPLVETKAGPKQQRKRSTSAFDTELTSTTKKALKQYRAGPSFEDRKPLGFPDRRKGGKFKTKSRYRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z456</accession>
    <name>A0A6I9Z456_9SAUR</name>
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        <submittedName>
            <fullName evidence="8 9 10">RING finger protein 32 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">RNF32</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Skeletal muscle</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014075957.1"/>
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        <property type="nucleotide sequence ID" value="XM_014075958.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING-H2_RNF32_rpt1"/>
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14991">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14991:SF0">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="139"/>
            <end position="181"/>
        </location>
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            <end position="95"/>
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    </feature>
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        <location>
            <begin position="72"/>
            <end position="85"/>
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        </source>
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        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013931434.1"/>
        </source>
    </evidence>
    <sequence length="375" mass="43678" checksum="D055AF63986A20A0" modified="2020-10-07" version="1">MMQKKYFKGSSYKPESVVLTAVALQDHILHDLQLQHLSLADPLKSKTWNKINKYRPVNKEKIKAVIDTGLKQTNKQQNNQNNPQNEDPEKEYVLDPSPQPLTLAQKFGLVELPDVPLTLDDWEYVKQRSIKQGDSVQPCAICKEEFALQPQVLLSCSHVFHRACLKAFERFSAKKSCPMCRKTQYQTRVIHDGARLFKIKCATRIQAFWRGHIVRKWYKNLRKTVPPKDVRLRKKFFEEKLTEICQRLLKSYDTCIDEFFCEIDSTVAASHEIFQQLEEKLGPEIHEVEWEKIQIQAFRQDISDCPICIMPLMQPAQLQDGLSPNVYQSCPRQTALLSCSHMFHSTCLQAFEEFSLRKRPICPLCRSCYQKKILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4W1</accession>
    <name>A0A6I9Z4W1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Erythroid transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GATA1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931011.1">
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GATA transcription factor (GATAd)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gata transcription factor gatad"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
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        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF190">
        <property type="entry name" value="ERYTHROID TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="184"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="238"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="GATA-type 1" evidence="11">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="GATA-type 2" evidence="11">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="291"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR003027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013931011.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="41340" checksum="637A73F10A210C89" modified="2020-10-07" version="1">MDYSGQDSAPQYMESSHLQGNDDSDLFFTSPDGIDCNAVPTYFPRMPESYRHSPVRQLYPLLNGLQWGDSHPYSTATWVSSASGKTHTGLPIYTSATSSYHTLHSPPSPKDLSPDGKSSPRYLEMLKTERISPTHAELLPLGTSTGTALSPYAMGQEYGYFPMPGSPLATGYSPKLRGTMQLSPSEARECVNCGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCSNCQTTTTTLWRRNMNGEPVCNACGLYYKLHNVNRPLAMRKDGIQTRNRKVSSAKSKKRKGNSGETAIPANTSLEMMPSSLMGEEAAALYPLSPMMLPGHLLPFGANPHFLGSPTSFPPQASPVPHGSPGVVSALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4X8</accession>
    <name>A0A6I9Z4X8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metastasis-suppressor KiSS-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">KISS1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931712.1">
        <property type="nucleotide sequence ID" value="XM_014076237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4X8"/>
    <dbReference type="GeneID" id="106557108"/>
    <dbReference type="KEGG" id="tsr:106557108"/>
    <dbReference type="CTD" id="3814"/>
    <dbReference type="OrthoDB" id="9899812at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031773">
        <property type="term" value="F:kisspeptin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020207">
        <property type="entry name" value="Metastasis-suppressor_KiSS-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16955">
        <property type="entry name" value="METASTASIS-SUPPRESSOR KISS-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16955:SF6">
        <property type="entry name" value="METASTASIS-SUPPRESSOR KISS-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15152">
        <property type="entry name" value="Kisspeptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026863634" evidence="1">
        <location>
            <begin position="20"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931712.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15731" checksum="46D633BAE7B2294F" modified="2020-10-07" version="1">MNLLCSLLMFLLFFRIQFGKPAGSYYPLKNFWPQAKPSRGKANWIHWANDIPCSERSFSSGEEGLKSTVTLLCKRQEHQVQLWPGLLPTKSKGVPLLEGALVLEGSQDLSTYNWNSFGLRYGKRQAVKAQEKSRKPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4Z0</accession>
    <name>A0A6I9Z4Z0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UBE2R2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013931274.1">
        <property type="nucleotide sequence ID" value="XM_014075799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4Z0"/>
    <dbReference type="GeneID" id="106556791"/>
    <dbReference type="KEGG" id="tsr:106556791"/>
    <dbReference type="CTD" id="54926"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23803">
        <property type="entry name" value="UBCc_UBE2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000009">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="12">
        <location>
            <begin position="8"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="193"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="206"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931274.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="27518" checksum="D4D7DDB8B377AA56" modified="2020-10-07" version="1">MAQQQVSSSQKALMLELKGLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVQATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEEEEDADCYDDDDSGNEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z515</accession>
    <name>A0A6I9Z515_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gamma-sarcoglycan</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SGCG</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <location evidence="3">Sarcolemma</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sarcoglycan beta/delta/gamma/zeta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931299.1">
        <property type="nucleotide sequence ID" value="XM_014075824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z515"/>
    <dbReference type="GeneID" id="106556813"/>
    <dbReference type="KEGG" id="tsr:106556813"/>
    <dbReference type="CTD" id="6445"/>
    <dbReference type="OrthoDB" id="8881719at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048738">
        <property type="term" value="P:cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006875">
        <property type="entry name" value="Sarcoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039972">
        <property type="entry name" value="Sarcoglycan_gamma/delta/zeta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12939:SF4">
        <property type="entry name" value="GAMMA-SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12939">
        <property type="entry name" value="SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04790">
        <property type="entry name" value="Sarcoglycan_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013931299.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="32755" checksum="7E036A88279C0B7F" modified="2020-10-07" version="1">MVREEYISATPGTDVQRPDPQYTYNVGIYGWRKRCLYLFLLLLIIILVVNLALTIWILKVMWFSTAGMGYLQVNSDGLRLEGESEFLYPLHAKEIHSRKDSPLLLQSSHNVTLNARNSDGNVTGRLSVDLDMVQFHSQLFQINSVNFDEKPLFTAHDSDTAIGTEKLRVTGPEGALCEHSVETPLVQGVGEDVKNLTLKLESPTRSLTMNAPRGIHIKAENGDLIGLSHLDIAFHSTNGMIIDAQNLLLPDLLQGTNGESGTSEGLYEICACPDGRLYLSVADVVSTCLQHSRDCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z566</accession>
    <name>A0A6I9Z566_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enhancer of polycomb homolog 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931111.1">
        <property type="nucleotide sequence ID" value="XM_014075636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z566"/>
    <dbReference type="GeneID" id="106556658"/>
    <dbReference type="KEGG" id="tsr:106556658"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009607">
        <property type="entry name" value="Enhancer_polycomb_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06752">
        <property type="entry name" value="E_Pc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enhancer of polycomb C-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931111.1"/>
        </source>
    </evidence>
    <sequence length="130" mass="13434" checksum="D3660E322BEB25FF" modified="2020-10-07" version="1">MPTALVHTSSSTAGSAFLQPSNIKQTLSSHSAVSHHVTAANSTTTQVLFGNNIRLTVPSSVATANSINILDACHMPRTLSAIPSSALKLAAANCQVPASSSVNTVSRKNHEPEKSALNNIADNTVAMEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z702</accession>
    <name>A0A6I9Z702_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Unconventional myosin-Id</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">MYO1D</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931919.1">
        <property type="nucleotide sequence ID" value="XM_014076444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z702"/>
    <dbReference type="GeneID" id="106557250"/>
    <dbReference type="KEGG" id="tsr:106557250"/>
    <dbReference type="CTD" id="4642"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000003">
        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000004">
        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000007">
        <property type="entry name" value="unconventional myosin-Id"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000009">
        <property type="entry name" value="Unconventional myosin-Id"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF417">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 19" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14 19" id="KW-0518">Myosin</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Myosin motor" evidence="20">
        <location>
            <begin position="9"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="21">
        <location>
            <begin position="805"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="19">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013931919.1"/>
        </source>
    </evidence>
    <sequence length="999" mass="115444" checksum="A63BDCE757BFD94D" modified="2020-10-07" version="1">MAELESLEFGKADFVLLDTVSMPEFMANLRLRFEKGRIYTFIGEVVVSVNPYKNLNIYGRDIVEQYKGRELYERPPHLFAIADAAYKAMKRRSKDTCIVISGASEIPATSPSGDTLNKAGRLKLERVKNMLLKSNCVLEAFGNAKTNRNDNSSRFGKYMDINFDFKGDPIGGHINNYLLEKSRVIVQQLGERNFHSFYQLLQGGSEQLLRSLYLHKDLSAYSYISAGTQPKCTINDSADFKAVADAMKVIGFGPEEVQTVYKILATILHLGNLKISVDGETPVIENGKLVSVIADLLSTKADTVEKALLFRTVATGRDIIDKQHTEQEATYGKNAFAKAMYERLFCWIVMHINDGIAVKDYNSTVHGKNTVIGVLDIYGFEIFDNNSFEQFCINYCNEKLQQLFIQLVLKQEQEEYQREGIPWKHIDYFNNQIIVDLVELQHKGIIANLDDACMNVGKVTDEMFLEALNKKLGKHAHFSSRKLCATDKNLEFDRDFRIKHYAGDVIYSVTGFIDKNKDTLFQDFKRLMYNSSNPVLKKMWPEGKLSITEVTKRPLTAATLFKNSMIALVDNLASKEPYYVRCIKPNEKKSPQLFDEERCKHQVEYLGLLENVRVRRAGYAYRQTYEKFLHRYKIIPEFTWPNHKLPSDKEAVKKLIEHCGFQDDVAYGKTKIFIRTPRTLFTLEEMHAKMLEWVVLFLQKVWRGTLARLQYKRTRAALKILKCYRRYKVKSYICEVARRFQNVKSMKDYGKHVKWPNPPKVLRRFEEALHAIYYRWRAHELIKNIPPKDLPQIKAKVAALELLKGHRGDIGLRRVWQGNYLALNPGNSQTNSSFTSVTSELQRKDKFMRILFSSQVRKVNRFNKVEDRAIFITDRHLYKMDPIKQYKVMKTIPLYNLTGLSISNGKDQLVVFHTKDNKDLIVCLVSMHSLNENRIGELVGVVANHFKSENRRLQVNITNPVQCSMHGRKCSISVKTKINQSEPNFIKDWTCFILWVPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYC7</accession>
    <name>A0A6I9WYC7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neurobeachin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106538079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013907949.1">
        <property type="nucleotide sequence ID" value="XM_014052474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYC7"/>
    <dbReference type="GeneID" id="106538079"/>
    <dbReference type="KEGG" id="tsr:106538079"/>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF111">
        <property type="entry name" value="NEUROBEACHIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907949.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="13138" checksum="2114C48BCB9E4C97" modified="2020-10-07" version="1">MTQGSPDMMVTVSANGLLGTHSWLPYDKNISNYFSFTKDSTVSNPKTQRYLYGPFAPGAELSSKTLVVSHDGKLLFSGGHWDNSLRVTSLSKGKVVGHINRHLGGLKANQQNFHGLKDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2V3</accession>
    <name>A0A6I9X2V3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WSC domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">WSCD2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909609.1">
        <property type="nucleotide sequence ID" value="XM_014054134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2V3"/>
    <dbReference type="GeneID" id="106539349"/>
    <dbReference type="KEGG" id="tsr:106539349"/>
    <dbReference type="CTD" id="9671"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF7">
        <property type="entry name" value="SIALATE:O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WSC" evidence="3">
        <location>
            <begin position="126"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013909609.1"/>
        </source>
    </evidence>
    <sequence length="406" mass="46110" checksum="4CBCA023C01D13FF" modified="2020-10-07" version="1">MAKLLLKFQRYFRRKPIRFFTLIALYLTAGSLVFLHAGFSGETRVPGAAPRPSNGAAEGTEAQSLGALPLGRELRAMPESLVLARRYGTWFKNPPKEAAGRSRGDYGETWSRVLKGKSALEKEEERAKYIGCYVDNTHQRALRGVSFFDYKKMTLFRCQDNCAERGYLFAGLEFGAECYCGHKILAENVSDSQCNMECKGEKSSFCGGPNRLSIYRLDLAQESTRRYGSAIFRGCFRRPENLSLALLASRPMKNMSVDRCVDFCTGKVHKPPLFLGTNAGSVEASFGLLEWPEFVKNYAPWWVTHTLDWLKFGKKVLVVHFEDLKQDLFVQLKRMVSLLGTAVHEDRLLCVEGQKDGNFKRSGLRKLEYDPYTPEMKKMINGYIKTVDMALKLRNLTGVPKDYYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X316</accession>
    <name>A0A6I9X316_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase delta subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA polymerase delta subunit p66</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106539418</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909684.1">
        <property type="nucleotide sequence ID" value="XM_014054209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X316"/>
    <dbReference type="GeneID" id="106539418"/>
    <dbReference type="KEGG" id="tsr:106539418"/>
    <dbReference type="OrthoDB" id="514823at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1030.20:FF:000001">
        <property type="entry name" value="DNA polymerase delta 3, accessory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.20">
        <property type="entry name" value="DNA polymerase delta, p66 (Cdc27) subunit, wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019038">
        <property type="entry name" value="POLD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041913">
        <property type="entry name" value="POLD3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598:SF13">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09507">
        <property type="entry name" value="CDC27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0228">DNA excision</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="173"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="224"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="268"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="304"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013909684.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="45313" checksum="17148B6AA0085758" modified="2020-10-07" version="1">MAAVEGRGGEGALWGSRGPGRPAMEDELYLENIDEFVTDQNRVVTYKWLSFTLGVHVNQAKQMLYDYVERKRKENVGAQLHVTYLLAGNLVQSGHTYHKVAVVREDKLEALKSKFSTVASVHVYSIQKAQLRDSSPLFNTDYDILKANLENCGKFSAICCPAAVIRSVAEDPQAENPAQLPPQPPPATSALLAPALNGHAPGASSKQPAQPMKGIMGMFAAKATSKTKNTNKEAKPETKETPSASVTNSKAAAKGNMLNNFFGKAAVSKEDKASAEPEQQKEERSAAKPPVSSTDPKPPLNPAGHEKASKEAEPARGQQRDKKRAKRADASDEEEKGSRRLKKKRRRIKPLQSDSSGGEEDSSSLATSEDTPPVPAQKPRTQPAPKPTEASVGVKRRKRKRVLKSRTFVDDEGCIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3J2</accession>
    <name>A0A6I9X3J2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Protein phosphatase 1B</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Protein phosphatase 2C isoform beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">PPM1B</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer. Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013908412.1">
        <property type="nucleotide sequence ID" value="XM_014052937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3J2"/>
    <dbReference type="GeneID" id="106538451"/>
    <dbReference type="KEGG" id="tsr:106538451"/>
    <dbReference type="CTD" id="5495"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.430:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.430">
        <property type="entry name" value="Phosphatase 2C, C-terminal domain suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012911">
        <property type="entry name" value="PP2C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036580">
        <property type="entry name" value="PP2C_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07830">
        <property type="entry name" value="PP2C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81601">
        <property type="entry name" value="Protein serine/threonine phosphatase 2C, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 24" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="26">
        <location>
            <begin position="23"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="426"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013908412.1"/>
        </source>
    </evidence>
    <sequence length="471" mass="52104" checksum="0630E5C53D5DB704" modified="2020-10-07" version="1">MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSNHLLEHITNNEDFRGAEQPGCALEPSVENVKSGIRTGFLKIDEFMRNFSDLRNGMDRSGSTAVGVMISPEHIYFINCGDSRAVLYRNGQVCFSTQDHKPCNPREKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEIVRAEEDEFIVLACDGIWDVMSNEELCEFVKSRLEVSDDLEKVCNWVVDTCLHKGSRDNMSIVLVCFSNAPKVSDEAMKRDADLDKHLESRVEEIMEKSGEEGMPDLAQVMRILSAENIPNLPPGGGLAGKRNIIEAVYNRLNPHRENEGEDDKAAGGATQGKLTDALRQVRITHRGNYRHLLEDVLISYRLAKIQGEESSGESATASTSDASNNQETDSKAKCEHQRAETQSPNEDSEMSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4A4</accession>
    <name>A0A6I9X4A4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AP-3 complex subunit beta-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106539001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909141.1">
        <property type="nucleotide sequence ID" value="XM_014053666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4A4"/>
    <dbReference type="GeneID" id="106539001"/>
    <dbReference type="KEGG" id="tsr:106539001"/>
    <dbReference type="OrthoDB" id="302453at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056314">
        <property type="entry name" value="AP3B1/2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24080">
        <property type="entry name" value="AP3B1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP-3 complex subunit beta-1/2 C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013909141.1"/>
        </source>
    </evidence>
    <sequence length="107" mass="11508" checksum="68FAD48822B47528" modified="2020-10-07" version="1">MTEKDFRKERGSLTGMNEVSATISVAPQNTTRLMILQKVTSIANLGGVPSGEDNIHRFAAKTVHSGSLVLVTVELEEGSTAQLFINTEKTVIGSILLRELKPVLSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X533</accession>
    <name>A0A6I9X533_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Non-histone chromosomal protein HMG-14A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106540045</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMGN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910499.1">
        <property type="nucleotide sequence ID" value="XM_014055024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X533"/>
    <dbReference type="GeneID" id="106540045"/>
    <dbReference type="KEGG" id="tsr:106540045"/>
    <dbReference type="OrthoDB" id="9049785at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000079">
        <property type="entry name" value="HMGN_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23087:SF13">
        <property type="entry name" value="NON-HISTONE CHROMOSOMAL PROTEIN HMG-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23087">
        <property type="entry name" value="NONHISTONE CHROMOSOMAL PROTEIN HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01101">
        <property type="entry name" value="HMG14_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00925">
        <property type="entry name" value="NONHISHMG17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00527">
        <property type="entry name" value="HMG17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013910499.1"/>
        </source>
    </evidence>
    <sequence length="107" mass="11597" checksum="A1B5A423DB84770A" modified="2020-10-07" version="1">MPKRKAAAEGEIKEEPKRRSARLSAVSKPAPPKPEPKPKKAPPKKEKGGNDKKEDKKVATKGKKGAKGKDETKQEDAKEENHSENGDTKTNEAPAAEVSDVKEAKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X617</accession>
    <name>A0A6I9X617_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PHD finger protein 21B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PHF21B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013909267.1">
        <property type="nucleotide sequence ID" value="XM_014053792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X617"/>
    <dbReference type="GeneID" id="106539108"/>
    <dbReference type="KEGG" id="tsr:106539108"/>
    <dbReference type="CTD" id="112885"/>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102:SF18">
        <property type="entry name" value="PHD FINGER PROTEIN 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="143"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909267.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="36508" checksum="428536D040D41587" modified="2020-10-07" version="1">MPINTSAAVSVVNDSIKVQPLLISADSKVIIIQPKVPTQTENKVEIKKPAEESTQGPATKKKKDENPEKIAFMVALGLVTTEYLEEIQSKRQERKRRSTANPAYSGLLETERKRLASNYLNNPFFLSTRANEDPCWKNEVSHEEYCSACKHGANLQPCGTCCRAYHLSCLDPPLKTVPKGVWVCPKCQEKVLKKDDNVPWSGTLAVVQSYVTHKTVKEEEKRRLLKTSSELRAEHRQLEEKECFLNNAMKKCLELKNSLLAQQKGVQSSLEHLKTLIRLIQNEQMLQVTMTTTTTSSSSLLTVPWTKSSSASAAAAPQPLQSTQGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X679</accession>
    <name>A0A6I9X679_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase</fullName>
            <ecNumber evidence="1">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106540393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013910959.1">
        <property type="nucleotide sequence ID" value="XM_014055484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X679"/>
    <dbReference type="GeneID" id="106540393"/>
    <dbReference type="KEGG" id="tsr:106540393"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013910959.1"/>
        </source>
    </evidence>
    <sequence length="80" mass="9393" checksum="D8E7CCBF61F6D334" modified="2020-10-07" version="1">MKVDSRANYTLMPPDILDFSKGNFLRKGTIGDWKNHMTVAQSERFDCVFKERMEKMPFKFCWDIQEDPEPISSSVDENNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7H1</accession>
    <name>A0A6I9X7H1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 572-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106539510</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909782.1">
        <property type="nucleotide sequence ID" value="XM_014054307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7H1"/>
    <dbReference type="GeneID" id="106539510"/>
    <dbReference type="KEGG" id="tsr:106539510"/>
    <dbReference type="OrthoDB" id="9034485at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000506">
        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000785">
        <property type="entry name" value="zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000862">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF162">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909782.1"/>
        </source>
    </evidence>
    <sequence length="224" mass="25224" checksum="D7C3DED69DAF2A62" modified="2020-10-07" version="1">MAMWTHTKEKLFECPECGKCFCDKSSLVTHQRTHTVEKPLECPDCGKCFSQRGILLRHQRTHTGEKTFQCPDCGKSFSHHSSLVSHQRTHTGEKPFECPDCSKSFSHNSTLVKHQRTHTGEKPFECPDCGKSFSQISSLVTHQRTHTGEKPFECPDCGKSFSHHSSLVTHQSTHTGEKHFECPDCGKGFSHNSSLVKHQRTHTGEKPFKCPDCGALKTIAVNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7I0</accession>
    <name>A0A6I9X7I0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed primase/polymerase protein</fullName>
            <ecNumber evidence="7">2.7.7.102</ecNumber>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PRIMPOL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:22509"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.102"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.102" evidence="7"/>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013910216.1">
        <property type="nucleotide sequence ID" value="XM_014054741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7I0"/>
    <dbReference type="GeneID" id="106539844"/>
    <dbReference type="KEGG" id="tsr:106539844"/>
    <dbReference type="CTD" id="201973"/>
    <dbReference type="OrthoDB" id="5988181at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009411">
        <property type="term" value="P:response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22256">
        <property type="entry name" value="PrimPol_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044917">
        <property type="entry name" value="PRIMPOL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31399">
        <property type="entry name" value="DNA-DIRECTED PRIMASE / POLYMERASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31399:SF0">
        <property type="entry name" value="DNA-DIRECTED PRIMASE_POLYMERASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03121">
        <property type="entry name" value="Herpes_UL52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013910216.1"/>
        </source>
    </evidence>
    <sequence length="626" mass="72785" checksum="F33A20FB1380463B" modified="2020-10-07" version="1">MNIGSTKRKWEEKLKNVEELTSCYKRRPLCSSYKPKLSNPLQPPSVWKLFYRQTHAFNFAKTCKEDVHVFALEKCDGNNQRLYLVTTYTELWFYYRKHETKLKHCYEIIPETAVCKLYFDLEFYKPTNQGANANQMVEDLIKFIGRKLDEYYEIKCSAENILKLDSSTEEKYSCHLIFLLENAAFKNNIHIGNFLRTILQPAILLTKNKDAMDPQNINSVGRCYKTTVGLPTCFETNSLIKDACQNLKLNSYKISEKVTSEENPDLSFLIVNGKQGEKQLFVDLGVYTKNRNFRLYMSSKAGHTVILNIAEDNKFVPKSVNNSCIDEQYFLSSLICNVRFSDCLKILTCDNQEHAREPSLHFEDKLSRSCLAPIEGCHCSPYPEIDDFVRTLIKKDNVQGGIRRWNYFSLEQLIVYDIFNYRWCRNIGRAHKSNNIMIIIDLKNENWYQKCHDPICRAENFKSECFPLPVEVCLPFLFKEDEEYVGTIGENNTEERANTVDTTGLLDSMPFTSMQENSKLNKKSSNVEWDHEFDACLLEASEDVELIEATDIFHTQRNCTVDEIPDELLADVLRNYEVKERKESYKNLVDEQHTCTQHAATCALPDETLLQLNNSKIIFLLERLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7X5</accession>
    <name>A0A6I9X7X5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Extracellular serine/threonine protein kinase FAM20C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106539975</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910396.1">
        <property type="nucleotide sequence ID" value="XM_014054921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7X5"/>
    <dbReference type="GeneID" id="106539975"/>
    <dbReference type="KEGG" id="tsr:106539975"/>
    <dbReference type="OrthoDB" id="8583677at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070166">
        <property type="term" value="P:enamel mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10314">
        <property type="entry name" value="FAM20_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF25">
        <property type="entry name" value="FAM20 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM20 C-terminal" evidence="9">
        <location>
            <begin position="308"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="344"/>
            <end position="347"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013910396.1"/>
        </source>
    </evidence>
    <sequence length="503" mass="57811" checksum="0E5BE95C3DE22430" modified="2020-10-07" version="1">MVRYLPFLLQRKLKATLCLFLLMVVLIHIVMEFVPSADKTSCRCLSKATGNLDHLSPRSISPTQNKMNLRNLQDFSSSNSSLEKGFHSTEGVSHVDGLSNVGLQDVNIKQQRETREKPSQKIKSKLSALFHHPLYKIPIPKVARKDKLFGNKPTMNLFIKNEGIIINSAEVLQNHSANPAWLSFYAGINRYELYARHDPSRHDLMKDLAKQKIISSVQKPGGTQLKLIMTFPNHGEALFKPMKQTRDQETSADFFYFSDFERHNAEISAFHLDRILDFRRIPPVSGRLVNISEDIRDITTDKKLFKTFFISPAGNICFFGECSYYCSTEHALCGKPDQLEGSMAALLPDKIIAGRQSWRNPWRRSYSKIKKAEWELNENYCAKIRKTPPYNNTARLLNLIDMTMLDFLMGNMDRHHYETFEKFGNHTFYLHLDNGRGFGRHSHDEMSILTPLRQCCIIKKSTFLRLQLLATEPFRLSDVMRESLASDPLSPVLSEPHLEALDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBE2</accession>
    <name>A0A6I9XBE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acylglycerol lipase</fullName>
            <ecNumber evidence="3">3.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ABHD6</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Lipase that preferentially hydrolysis medium-chain saturated monoacylglycerols including 2-arachidonoylglycerol. Through 2-arachidonoylglycerol degradation may regulate endocannabinoid signaling pathways. Also has a lysophosphatidyl lipase activity with a preference for lysophosphatidylglycerol among other lysophospholipids. Also able to degrade bis(monoacylglycero)phosphate (BMP) and constitutes the major enzyme for BMP catabolism. BMP, also known as lysobisphosphatidic acid, is enriched in late endosomes and lysosomes and plays a key role in the formation of intraluminal vesicles and in lipid sorting.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-dodecanoylglycerol + H2O = dodecanoate + glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:75539"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes glycerol monoesters of long-chain fatty acids.</text>
            <dbReference type="EC" id="3.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Late endosome membrane</location>
            <topology evidence="13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Lysosome membrane</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Mitochondrion membrane</location>
            <topology evidence="15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013911157.1">
        <property type="nucleotide sequence ID" value="XM_014055682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBE2"/>
    <dbReference type="GeneID" id="106540528"/>
    <dbReference type="KEGG" id="tsr:106540528"/>
    <dbReference type="CTD" id="57406"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000082">
        <property type="entry name" value="monoacylglycerol lipase ABHD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF5">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE ABHD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="19">
        <location>
            <begin position="75"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013911157.1"/>
        </source>
    </evidence>
    <sequence length="287" mass="32691" checksum="5418A5F4AABB8873" modified="2020-10-07" version="1">MDLDMLNMFVIAGGTLAIPILAFVVSFFLYPSALIKIYYWYWRRILGLQVKYAHCGDYQFCYCYRGRSSHRPSTLMLHGFSAPKEMWVSMVKFLPKNLHLICVDMPGHGGTTRSSLDDYSIYGQVKRIHQFVECINLNRKPFHLVGTSMGGHVAGVYAAQYPAEVCCLTLICPGGLVNSQESNFIKQLREQKENNCIDHIPLIPSTPEEMGDMLKLCSYVRFKVPHQILQGLVDVRIPHNDFYRKLFLELMDDKSLGCLHNNMSKIKATTQIIWGKQDQVLDVSGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCB0</accession>
    <name>A0A6I9XCB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Presenilin</fullName>
            <ecNumber evidence="11">3.4.23.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">PSEN2</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis. Is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and modulates calcium ions shuttling between ER and mitochondria.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PAL motif is required for normal active site conformation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase A22A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013908802.1">
        <property type="nucleotide sequence ID" value="XM_014053327.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908803.1">
        <property type="nucleotide sequence ID" value="XM_014053328.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908804.1">
        <property type="nucleotide sequence ID" value="XM_014053329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCB0"/>
    <dbReference type="GeneID" id="106538735"/>
    <dbReference type="KEGG" id="tsr:106538735"/>
    <dbReference type="CTD" id="5664"/>
    <dbReference type="OrthoDB" id="20287at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070765">
        <property type="term" value="C:gamma-secretase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034205">
        <property type="term" value="P:amyloid-beta formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.100:FF:000001">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.100">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001493">
        <property type="entry name" value="Pept_A22A_PS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001108">
        <property type="entry name" value="Peptidase_A22A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042524">
        <property type="entry name" value="Presenilin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10202">
        <property type="entry name" value="PRESENILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10202:SF24">
        <property type="entry name" value="PRESENILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01080">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01072">
        <property type="entry name" value="PRESENILIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01074">
        <property type="entry name" value="PRESENILIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="2 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="313"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013908802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013908803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013908804.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="51834" checksum="1284F381D96FA5E1" modified="2020-10-07" version="1">MIAFMNNSDSEEEPCNERTSLMSAESPLLPSYHDGAQASEATETLSHRNRRPGSTSSSNKAEGNGPDPERAAKGHIVLDNEEEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVRFYTEKNGQLIYTPFSEDTPYVGQRLLSSVLNTLIMISVILVMTIFLVLLYKYRCYKFIHGWLILSSLMLLFLFTYIYLGEVLKTYNVAMDYPTLFFVIWNFGAVGMICIHWKGPLKLQQAYLIMISALMALVFIKYLPEWSAWVILGGISVYDLIAVLCPKGPLRMLVETAQERNEPIFPALIYSSAMMWTIGMAKPDSAQRRSSQEIRDPEAETHDNSSYAEPEVPEMRNSTIDQTGQQLGQIACNVEESQEEEERGVKLGLGDFIFYSVLVGKAAATASGDWNTTLACFVAILIGLCLTLLLLAVFKKALPALPISITFGLIFYFSTDYLMQPFMDTLAAHQLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XED3</accession>
    <name>A0A6I9XED3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Neuronal acetylcholine receptor subunit alpha-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CHRNA3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914004.1">
        <property type="nucleotide sequence ID" value="XM_014058529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XED3"/>
    <dbReference type="GeneID" id="106542719"/>
    <dbReference type="KEGG" id="tsr:106542719"/>
    <dbReference type="CTD" id="1136"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000008">
        <property type="entry name" value="Cholinergic receptor nicotinic alpha 6 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000017">
        <property type="entry name" value="Neuronal acetylcholine receptor subunit alpha-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="7"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="220"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013914004.1"/>
        </source>
    </evidence>
    <sequence length="479" mass="55499" checksum="7D0460A6877C6B90" modified="2020-10-07" version="1">MFCSDDEHRLYKELFANYNKIIRPVENVSDPVMVWFEVSLSQLVKVDEVNQIMETNLWLKHIWNDYKLKWDPEKYGGVQYVRVPSNKIWKPDIMLYNNAIGDFQVDDKTKALLNYTGNVTWMPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAQIDLVLVGSAMNLKDYWESGEWAIIKAPGYKHDMKYNCCEAIYPDITYSLYMRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMLFVTLSIIITVFVLNVHYRTPRTHTMPDWVKVVFLRFLPRVMFMTRPPSNEDEGRPEKGKASSHVELAPLNLIQVPDSRYCQDCFLCQGFTHMCCLHAHRTHSKATGNLTQSSSSESMDALFPSPLISPEMRDVMDSIKYIAENMKLQNRAKETQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLLIRDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG21</accession>
    <name>A0A6I9XG21_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">cGMP-dependent protein kinase 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106542029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913106.1">
        <property type="nucleotide sequence ID" value="XM_014057631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG21"/>
    <dbReference type="GeneID" id="106542029"/>
    <dbReference type="KEGG" id="tsr:106542029"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF68">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913106.1"/>
        </source>
    </evidence>
    <sequence length="97" mass="10972" checksum="B1E13A0D3FBCBE7F" modified="2020-10-07" version="1">MDDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGT6</accession>
    <name>A0A6I9XGT6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LGALS3</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910243.1">
        <property type="nucleotide sequence ID" value="XM_014054768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGT6"/>
    <dbReference type="GeneID" id="106539861"/>
    <dbReference type="KEGG" id="tsr:106539861"/>
    <dbReference type="CTD" id="3958"/>
    <dbReference type="OrthoDB" id="8942303at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001772">
        <property type="term" value="C:immunological synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048030">
        <property type="term" value="F:disaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019863">
        <property type="term" value="F:IgE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048245">
        <property type="term" value="P:eosinophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048246">
        <property type="term" value="P:macrophage chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002548">
        <property type="term" value="P:monocyte chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045806">
        <property type="term" value="P:negative regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050918">
        <property type="term" value="P:positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090280">
        <property type="term" value="P:positive regulation of calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000023">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF26">
        <property type="entry name" value="GALECTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0389">IgE-binding protein</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10 19" id="KW-0430">Lectin</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="17" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Galectin" evidence="21">
        <location>
            <begin position="195"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013910243.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="33029" checksum="26CAF1650DCFAE2C" modified="2020-10-07" version="1">MTAEKKMSEGFSLSDALSGSNNPNPNMQTNPWGNQPGGFSGYPGAPGAFPGAPGAFPGAPGGYPGAPGAYPGAPGAYPGAPGAFPGAPGAFPGAPGAFPGAPGAFPGAPGALPGTPGALPGAPGTYPGAPGTCPGGIAAPGAYFGGPSGPGAFSNIPGSSGMYPAPGQPPSDRGAQPTAPQGGPGGFSGALKVPFDLPLSSGLVPRLLITVTGTVNPSPNRFQLDFKKGDDVAFHFNPRFNESNRKVIVCNSKIHNVWGTEDRTAPRFPFEAGKPFKIQILCEADQLKVAVDDAHLMQYKHRIKELQQITKLSVAGDVTLTSVMPFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH68</accession>
    <name>A0A6I9XH68_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hippocampus abundant transcript-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HIATL1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915054.1">
        <property type="nucleotide sequence ID" value="XM_014059579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH68"/>
    <dbReference type="GeneID" id="106543537"/>
    <dbReference type="KEGG" id="tsr:106543537"/>
    <dbReference type="CTD" id="84641"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17387">
        <property type="entry name" value="MFS_MFSD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF32">
        <property type="entry name" value="HIPPOCAMPUS ABUNDANT TRANSCRIPT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="76"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="494"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013915054.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="56445" checksum="50B69986B95CAA55" modified="2020-10-07" version="1">MSPEPPPPSLPPPPPTPGGREAEVVALTVAAAAVVESEAVASGASGGRKCGSRIMLVRRIGFKPGGSASQGIGRPSVYHAVIVIFLEFFAWGLLTTPMLTVLHKTFPQHTFLMNGLIQGVKGFLSFMSAPLIGALSDVWGRKYFLLITVFFTCVPIPLMKISPWWYFAMISVSGIFAVTFSVIFAYVADITQEHERSTAYGLVSATFAASLVTSPAIGAHLSNLYGDNMVVLVATVVAVVDICFILLVVPESLPEKMRPPSWGALISWEQADPFASLRKVGKDSTILLTCITVFLSYLPEAGQYSSFFLYLRQVIKFEYASIATFIAVIGILSIVAQTVFLSILMKTIGNKNTVLLGLGFQMFQLAWYGFGSQPWMIWAAGAVASMSSITFPAISALVSRNADSDQQGVVQGIITGIRGLCNGLGPALYGFIFFLFHVELNDLAPVQDKAAKQDPSDESSVIPGPPFLFGACAVLLAFLVALFIPEHSKAANTRKHSNSINSIQSNSPDRDSDEDIEPLLQDSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHL6</accession>
    <name>A0A6I9XHL6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Meckelin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106542231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913372.1">
        <property type="nucleotide sequence ID" value="XM_014057897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHL6"/>
    <dbReference type="GeneID" id="106542231"/>
    <dbReference type="KEGG" id="tsr:106542231"/>
    <dbReference type="OrthoDB" id="419138at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019170">
        <property type="entry name" value="Meckelin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274">
        <property type="entry name" value="MECKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09773">
        <property type="entry name" value="Meckelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013913372.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="29397" checksum="AB7A5468368A719F" modified="2020-10-07" version="1">MMQKVFLGGTSTCVDLFQWIGRSQQGFLQEGSLDSCGKLTCLTVPQVFLVLFQMLVSVGLYQRFVEDKIHQFVDLCSLSNVSVFILTHRCYGFYIHGRSIHGHADVSLDTMLSYLKKEEDNLCPLRGLEPNSETQTFEVLLTDQTRRFYDRILLSLMEHQRGLHSRPDLHQQRMKGYHALNWFLVSFLEHRYNDMDYIVKDKFFSERIMDLEFQEQGDLSVLYNGTHHALSYGDLSFKGWSHDSGCRWRGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHU2</accession>
    <name>A0A6I9XHU2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">tRNA-splicing endonuclease subunit Sen2</fullName>
            <ecNumber evidence="3 9">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17" type="primary">TSEN2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_013913457.1">
        <property type="nucleotide sequence ID" value="XM_014057982.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913459.1">
        <property type="nucleotide sequence ID" value="XM_014057984.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913460.1">
        <property type="nucleotide sequence ID" value="XM_014057985.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913461.1">
        <property type="nucleotide sequence ID" value="XM_014057986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHU2"/>
    <dbReference type="GeneID" id="106542301"/>
    <dbReference type="KEGG" id="tsr:106542301"/>
    <dbReference type="CTD" id="80746"/>
    <dbReference type="OrthoDB" id="10249562at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000001">
        <property type="entry name" value="tRNA-splicing endonuclease subunit Sen2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.20">
        <property type="entry name" value="tRNA intron endonuclease, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016589">
        <property type="entry name" value="tRNA_splic_SEN2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011789">
        <property type="entry name" value="tRNA_splic_SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15 16" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="14 15 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14 15 16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease N-terminal" evidence="12">
        <location>
            <begin position="268"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="11">
        <location>
            <begin position="324"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR011789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR011789-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013913459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013913460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013913461.1"/>
        </source>
    </evidence>
    <sequence length="450" mass="52477" checksum="1A7AA42A3BCA2843" modified="2020-10-07" version="1">MAEAIFHPPRRKRKVYESYDSPFPISPSVEGNHGRVYKMCRAELINNNVILKNPEDIEQLYSKGFFGKGILSRSRPEYNISDPMLTAKWKDAKLNMPIISSQKYQRCVEWAKNRLSEQGLDDSTINNIIQNYTKQINLRIETGQNNGVQDEETFKMEANKERTLLMASSEINDNNRSFKDNSRDSLDKHECSESDITDSNILAKTPSPQRCILNMYDSCREDNYVEPESHCFSNNEYEHELVLIEEKESDLCMKEETSEELHLIKKANLVCRRNPFWIFEYLQLSLEEAFFLVYALGCLNIYCNEAPLSILTLWDLFSKAQPNFKATYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIAELINDDFDGPLHRPFSWKTLSGLNRTTANVSKELMFCYLIKPSDMNEKEMLSPECLKRMKVQELILNRWVSSCERSEQEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI55</accession>
    <name>A0A6I9XI55_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ceramide synthase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CERS1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>sphinganine + octadecanoyl-CoA = N-(octadecanoyl)-sphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:67033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:36548"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915444.1">
        <property type="nucleotide sequence ID" value="XM_014059969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI55"/>
    <dbReference type="GeneID" id="106543865"/>
    <dbReference type="KEGG" id="tsr:106543865"/>
    <dbReference type="CTD" id="10715"/>
    <dbReference type="OrthoDB" id="537032at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF58">
        <property type="entry name" value="CERAMIDE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="9">
        <location>
            <begin position="2"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013915444.1"/>
        </source>
    </evidence>
    <sequence length="247" mass="28272" checksum="95B78BC42ECC5092" modified="2020-10-07" version="1">MDIASLWSGVIVILCILDATGVLSSPSLLFLPGFPDWEKGMQVPLDIALAYLTQGSFYAHSIYATLYMDAWRKDSVVMLIHHVVTLTLIAFSYLFRYHNIGILVLFLHDFSDIQLEFTKLNVYFKYRGGIYHRLNDTLSNAGCVSFSVSWAVLDLRPQLSPRFLLLNKTVPKSFASFKADWFLLFFSRQYIVVFVAKVLLGQVQEVKDVREYDTDAVSKKTGKELHLHNSWKEGNHLKNGIVKDKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ40</accession>
    <name>A0A6I9XJ40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 7">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CA14</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 7"/>
    <dbReference type="RefSeq" id="XP_013910933.1">
        <property type="nucleotide sequence ID" value="XM_014055458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJ40"/>
    <dbReference type="GeneID" id="106540371"/>
    <dbReference type="KEGG" id="tsr:106540371"/>
    <dbReference type="CTD" id="23632"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000003">
        <property type="entry name" value="Carbonic anhydrase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF84">
        <property type="entry name" value="CARBONIC ANHYDRASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027161556" description="Carbonic anhydrase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013910933.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="35286" checksum="3C8AB1FB5BA429E6" modified="2020-10-07" version="1">MLLVLSLWQALSQALAASGGSHWTYEGSEGQTHWAERFPDCGWQAQSPLDFQTDLVQYDPSLPPIEPEGYRDPGSGSLTLTNNGHTVQLSLPENLHLHGLPSLYSAAQLHLHWGGSSNSRAGGSEHLVDGQAFPAELHVVHFNSEKYANLSVAKHQPDGLAVLGIFLEVDSVVENPAYNHILDRLDSILYAEQEVTVAPFSVRELLPAHLGHYYRYSGSLTTPPCYQSVLWTVFRQPARISAAQLEKLQKSLYSTKRGKTPSKLLVSNFRVPQSLNQRLVLASFPVGPSDYSAGEVIAIIVAVLLGCLGVFLAGWILAKRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJI0</accession>
    <name>A0A6I9XJI0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">WD repeat-containing protein 72</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">WDR72</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914186.1">
        <property type="nucleotide sequence ID" value="XM_014058711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJI0"/>
    <dbReference type="GeneID" id="106542866"/>
    <dbReference type="KEGG" id="tsr:106542866"/>
    <dbReference type="CTD" id="256764"/>
    <dbReference type="OrthoDB" id="338622at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000247">
        <property type="entry name" value="WD repeat-containing protein 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049916">
        <property type="entry name" value="WDR72-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057848">
        <property type="entry name" value="WDR72_alpha-sol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44099">
        <property type="entry name" value="RABCONNECTIN-3B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44099:SF2">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23123">
        <property type="entry name" value="WDR72_alpha-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="58"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="466"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="614"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="665"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013914186.1"/>
        </source>
    </evidence>
    <sequence length="972" mass="107028" checksum="79B7C9B311DF900B" modified="2020-10-07" version="1">MRDSVQMVTLWGKEAPPHSITAILITDDQRTIVTGSHEGQVCLWDLLPDLQMSSRQMLLGHTASVTCLAKARDFEKQSYVVSATENGEMSIWDITSGRCVENTRLPFRHTAICYYHSSFRMAGDGWLVCCGQYNDILIIDAITLVVLHTLTSSSTSDWISCMCLVHSLRIQEDSLLAVSAAGDLKAWDLSSSISRIQEKQKIFERECQPLGCTPCMAIRFCTYTERLLLVVFSTCWKVYDYCDFCLLWTEFSPSSQALAGGEILAAHRLIIWTEDGHGYIYQLVNSGLSRSIQPSKGRVLKETVHPVLLSSTEVQGNKSFSYVMGFMNERKEPFYKILYSGQASGRITLWHVPDVPVSTLDGSPKVIPVAASRTLQDNFNFYHPVAEGTPDRPWGAPITVSTYIPSLQKLVCGYKDGRIITVPALHAAKARLLGEASVQKDAAAARVLSGASGSGVTNAGKVKWRPAAVNIHLPSPLCLGSLERHESGETAKAILVSCEDSGLMADPLYAAWEEDGQGEKGLGGSLSSSSAFGSFSCNRLAHQRRSFCHQLTALCWAQGPFAVWPLRATWETMSVNMLLFDLEKLVELLCSSQANGLKSSNSFSTIDTLKRARSATDKRTSMLKRNRTSGSLSPLDGQGRVASEEQGSGEVHTGGTQVEPSDGIKRRKKAKSSKKTKRQTSRKTSPTVATEAAKLLLSCFFPWGVNRELDGLCVQYLGILRLFSPVSFGLLSKNNYLTLMLPGWNSANPERMEKQQEVVNLFSSKVLHLHSKYYSATQEQAGKKNGWRNTVTSTEQRLVLAYLLSRMSLVQRIINRPSEKTSLMKRNKWRPTASVVLDDLNRESSPGMFRFPVCEADNRSFVKLISCWRDQSVEVMEATQATLLAEVEQSMQQAALAGGCGQTNSTRWGSERAKNPPSLDAAFGTIGMIKCENCITTPNVEEDGEGSAEELGIPGVSNPLPWVSKLCSCKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKK9</accession>
    <name>A0A6I9XKK9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rap1 GTPase-activating protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914546.1">
        <property type="nucleotide sequence ID" value="XM_014059071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKK9"/>
    <dbReference type="GeneID" id="106543152"/>
    <dbReference type="KEGG" id="tsr:106543152"/>
    <dbReference type="OrthoDB" id="2499658at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003109">
        <property type="entry name" value="GoLoco_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF3">
        <property type="entry name" value="RAP1 GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02188">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00390">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50877">
        <property type="entry name" value="GOLOCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914546.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="20749" checksum="6ECA6A3523B6799C" modified="2020-10-07" version="1">MADPLPKPPQWRGADEDPGGWSNTELFAMIEKMQANRMDEQRCAFPPPLKTEEDYIPYPSVHEVLGRESPFPLILLPQFGGYWIEGTNHDFAELSELQSPGCKVKLEGNHLARVYRKHFLGKRGSRSHSAPMLLSQGVGVELSPTCLTSLPQGPYKQATHALKPALPCSGSSSIPVQQDPMWPRTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKN5</accession>
    <name>A0A6I9XKN5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Gamma-tubulin complex component 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TUBGCP3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914512.1">
        <property type="nucleotide sequence ID" value="XM_014059037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKN5"/>
    <dbReference type="GeneID" id="106543126"/>
    <dbReference type="KEGG" id="tsr:106543126"/>
    <dbReference type="CTD" id="10426"/>
    <dbReference type="OrthoDB" id="5860513at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="247"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="551"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914512.1"/>
        </source>
    </evidence>
    <sequence length="903" mass="103980" checksum="53A088A456D32076" modified="2020-10-07" version="1">MASPDQKSPNVLLQNLCCRILGKGEADVAQQFQYAVRVIGSNFAPTVERDEFLVAEKIKKEFVRQGREEHAAVFSELYRKLYSQGVLKNRWSILYLLLSLSEDPRKQSNKVSNFATLFAQALPRDAHSTPYYYARPQSLPLNYQDRNLQPGTMGSSGISSIGIYAPNGPTPTPPTFLPGQSYQRAGVGDCLRQQLGTRLAWTLTAGQPTLQNSTSKGLSTTVSRNQPRSRREGDISGSIEITEAYLVRDILYVFQGIDGKIIKMFNSENCYKVDAKANLCKSLRDTTSRLAELGWLHNKIRKYTDQRSMDRAFGLVGQSFCAALNQELKEYYRLLSVLHSQLQVEDDQGVNLGIESSLTLRRLLVWTYDPKIRLKTLAALVDHCHGRKGGELASAVHAYSKTGDPYMKSLVQHILGLVSHPILNFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIPSFITMDHSRKVLLIGKSINFLHQVCHDQSPTSKMLAVPRSAESPKDAAEMFTDLENAFQGKIDAAYFETSKYLLDVLHKKYNLLEHMQAMRRYLLLGQGDFIRHLMDLLKPELARPATTLYQHNLTGILETAVRATNAQFDNPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATMFTRECMSHYLRVFNFLWRAKRMEYILTDIWKGHMCNAKLLKNMPELSGVLHQCHVLASEMVHFIHQMQYYITFEILECSWDELWKKVQQAQDLDHIIAAHEVFLDTIIARCLLDTDSRMLLNQLRAVFDQILEFQNVQDTMYRAALEELQLRLQFEERKKEREVEGEWGVTASEQEEEMKRMKEFEDSIPKMCSQLRLLTHFYQGIVQQFLVLLTTSSDESLQFLSFRLDFNEHYKAREPRLRVSLGTRGRRNSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPL1</accession>
    <name>A0A6I9XPL1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Rho GTPase-activating protein 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Rho-type GTPase-activating protein 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ARHGAP24</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with FLNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917949.1">
        <property type="nucleotide sequence ID" value="XM_014062474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPL1"/>
    <dbReference type="GeneID" id="106545813"/>
    <dbReference type="KEGG" id="tsr:106545813"/>
    <dbReference type="CTD" id="83478"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035021">
        <property type="term" value="P:negative regulation of Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900028">
        <property type="term" value="P:negative regulation of ruffle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035313">
        <property type="term" value="P:wound healing, spreading of epidermal cells"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04390">
        <property type="entry name" value="RhoGAP_ARHGAP22_24_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000015">
        <property type="entry name" value="rho GTPase-activating protein 25 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF19">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="33"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="20">
        <location>
            <begin position="149"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="371"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="616"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="665"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="446"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="631"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013917949.1"/>
        </source>
    </evidence>
    <sequence length="761" mass="85865" checksum="705323AF4213DC40" modified="2020-10-07" version="1">MGNSRTKGEAADFIMEEHNGSAESSLLSPARHVAIKCGWLRKQGGFVKTWHTRWFVLKGEMLYYFKDEDEAKPLGTIFLPGNRVVEHPSSDENPGKFLFEVVAGGDRERMTSNHETYLLMASTQNDMEDWVRSIRRVIWAPFGGGIFGQKLEDTVRYEKRYGNHLAPLLVEQCVDFIRLRGLKEEGLFRLPGQANLVKELQDAFDCGEKPLFDRNTDVHTVASLLKLYLRELPEPVIPYSKYEEFLSCAKMLSKEKETGLNELAKQVKNLPLVNFNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKVEDPLTIMEGTVVVQQLMSMMISEHELLFPKDAESQSGQEVCNNNNEVQKKVIIGQLQNRENNNTKESSPVRRCSWDKPESPQRTLVDNGSPTAQPGSKSNSPRNSLHKLDMTRSPPLTVKKNPAFNKGSGIVTNGSFSSSVESQEKTSPSLPNGTLQTRRTSSLKGPVTKMGTHSVPNGGVRMGISSTDAHSNSLNSRCQSWMPNGYVTLRDNKQKEPMSDSGQHNRLSTYDNVHQQFSAVNAEDKQSVDSATWSTSSCEISLPEHSNSCRSSTTTCPEQDFYGSNFEDPVLDEHQQDNLLNPAEGESKVDRRSVGGRSSRATSSSDNSETFVNNTGNHSALHSLVSSLKQEMTKQKLEYETRIKSLEQRNLSLETEMMNLHDELDQEKKKFTMVEIKMRNAERAKDDAEKRNDMLQKEMEQFFSTFGELTVEPRRTERGNTIWIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQG4</accession>
    <name>A0A6I9XQG4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CD63</name>
        <name evidence="10" type="synonym">LOC106550367</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916031.1">
        <property type="nucleotide sequence ID" value="XM_014060556.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923744.1">
        <property type="nucleotide sequence ID" value="XM_014068269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQG4"/>
    <dbReference type="GeneID" id="106544314"/>
    <dbReference type="GeneID" id="106550367"/>
    <dbReference type="KEGG" id="tsr:106544314"/>
    <dbReference type="KEGG" id="tsr:106550367"/>
    <dbReference type="CTD" id="967"/>
    <dbReference type="OrthoDB" id="10033535at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900746">
        <property type="term" value="P:regulation of vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF456">
        <property type="entry name" value="CD63 MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013923744.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="26426" checksum="33B43683FE3A5BBB" modified="2020-10-07" version="1">MGVEGGMKCVKYLMFFFNFIFWLCGIALIALGIWVQIELKNTLTMTSSTSTTAVPIVILSVGVIVFFISFLGCCGAAKENYCMVTTFAVLLTLIFLVEIAAAITGYIYKDKVQTVIKNEIEEEMNHYNQTYVKKALDGLQKKFSCCGINRYSDWFNITDIKPLKVPASCCINATDCTKNPTPENTFEEGCVKKIEDWLRKHIVIVAAVALGIAFFELLGIIFACCLMKGIRSGYEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRT3</accession>
    <name>A0A6I9XRT3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch-like protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KLHL10</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918759.1">
        <property type="nucleotide sequence ID" value="XM_014063284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRT3"/>
    <dbReference type="GeneID" id="106546414"/>
    <dbReference type="KEGG" id="tsr:106546414"/>
    <dbReference type="CTD" id="317719"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18450">
        <property type="entry name" value="BACK_KLHL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18240">
        <property type="entry name" value="BTB_POZ_KLHL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030608">
        <property type="entry name" value="KLHL10_BTB/POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF172">
        <property type="entry name" value="KELCH-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="37"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013918759.1"/>
        </source>
    </evidence>
    <sequence length="556" mass="63309" checksum="ECF5528E0E3347B6" modified="2020-10-07" version="1">MDDTSASAGYSYRHMERKMSAMACTVFNELRLEGKLCDVIIKVNGCEFNAHKNILCSCSSYFRALFTSSWNNSEKKIYNIPGITPDMMKLIIEYAYTRTVPITVDNVERLLVAADQFNVMGIVRACSDFMKSQLCLENCIGICKFTEYYYCPELRQAAYMFILHNFEEMVKASTEFLELSVNEFKDIIEKDELNVKQEDAVFEAILKWIAHEPPNRKQHMAVLLPKVRLALMHAEYFMNNVKMHDYVKDSEECKPIVIDALKAMYDLNMNGPSSSDFTNPLTRPRLPYAILFAIGGWSGGSPTNAIETYDARADRWVNVTCQQESPRAYHGAAYLKGYVYIIGGFDSVDYFNSVKRFEPIKKTWHQVAPMHSRRCYVSVTVLDNFIYAMGGFDGYVRLNTAERYEPETNQWTLIAPMHEQRSDASATTLYGKVGGFDGANRLRSAEAYNPIANTWRTIPTMFNPRSNFGIEVVDDLLFVVGGFNGFTTTFNVECYDEKTDEWYDAHDMSIYRSALSCCVVPGLPNVGEYAARRDNYVGSSQRDEVKYSSSTSTLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRV1</accession>
    <name>A0A6I9XRV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeodomain transcription factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106544903</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916782.1">
        <property type="nucleotide sequence ID" value="XM_014061307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRV1"/>
    <dbReference type="GeneID" id="106544903"/>
    <dbReference type="KEGG" id="tsr:106544903"/>
    <dbReference type="OrthoDB" id="10066656at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039775">
        <property type="entry name" value="PHTF1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021980">
        <property type="entry name" value="PHTF1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12680:SF2">
        <property type="entry name" value="PROTEIN PHTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12680">
        <property type="entry name" value="PUTATIVE HOMEODOMAIN TRANSCRIPTION FACTOR PHTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12129">
        <property type="entry name" value="PHTF1-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PHTF1/2 N-terminal" evidence="7">
        <location>
            <begin position="20"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916782.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25702" checksum="CF892A37F536C46B" modified="2020-10-07" version="1">MMMMMIQSWHLRWLNDEWLLHLCPLFFFLTVAAIILFFTIQTPHNIPLTEVIGPTWLMLLLGTVHCQIVSTRTPKSSTNTGGRRRRKLRKAAHLEVHREGDGSSTTDNTQEGAVQSYGTSTSSSLGAVFKELWHATFFLSGAKKAKNSIDKSTETDNGYVSLDGKRTGRNNEDALQSHEPLSETIRSEEPGWNIGTARSTYNTSNPKDSHRTMALTFSSPGFELWNQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS24</accession>
    <name>A0A6I9XS24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">hydroxyacid-oxoacid transhydrogenase</fullName>
            <ecNumber evidence="4">1.1.99.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106544722</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-3-hydroxybutanoate + 2-oxoglutarate = (R)-2-hydroxyglutarate + acetoacetate</text>
            <dbReference type="Rhea" id="RHEA:23048"/>
            <dbReference type="ChEBI" id="CHEBI:11047"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="EC" id="1.1.99.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4-hydroxybutanoate + 2-oxoglutarate = (R)-2-hydroxyglutarate + succinate semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:24734"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16724"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="EC" id="1.1.99.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.24" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013916551.1">
        <property type="nucleotide sequence ID" value="XM_014061076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XS24"/>
    <dbReference type="GeneID" id="106544722"/>
    <dbReference type="KEGG" id="tsr:106544722"/>
    <dbReference type="OrthoDB" id="339764at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047988">
        <property type="term" value="F:hydroxyacid-oxoacid transhydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08190">
        <property type="entry name" value="HOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000003">
        <property type="entry name" value="Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042157">
        <property type="entry name" value="HOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="9">
        <location>
            <begin position="44"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013916551.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="25810" checksum="BDA6DA94205D2508" modified="2020-10-07" version="1">MPERVAANSGFDVLCHALESYTALPYNKRSPCPSNPVNRPAYQGSNPISDVWAVHALQIVSKYLKRAIKNPEDQEARFNMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKNYKAKDYNVDHSLVPHGLSVILTSPAVFTFTAPMCPERHLEAAQIMGANISNAKMKDAGLILADTLRKILFDLNVDDGLAALGYSKADIPALVKGTLPQERVTKLSPRPQTEEELSSLFEASMKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSC9</accession>
    <name>A0A6I9XSC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hermansky-Pudlak syndrome 4 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HPS4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916651.1">
        <property type="nucleotide sequence ID" value="XM_014061176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSC9"/>
    <dbReference type="GeneID" id="106544789"/>
    <dbReference type="KEGG" id="tsr:106544789"/>
    <dbReference type="CTD" id="89781"/>
    <dbReference type="OrthoDB" id="16754at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031085">
        <property type="term" value="C:BLOC-3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903232">
        <property type="term" value="P:melanosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026091">
        <property type="entry name" value="HPS4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407:SF9">
        <property type="entry name" value="BLOC-3 COMPLEX MEMBER HPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME 4 PROTEIN LIGHT-EAR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="1">
        <location>
            <begin position="89"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="2">
        <location>
            <begin position="395"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916651.1"/>
        </source>
    </evidence>
    <sequence length="503" mass="55976" checksum="294516AC75FB9B90" modified="2020-10-07" version="1">MIEERVEWRCRGECERSFPAEFQEWIEKVIGWLSRVFLQESAGESLPSSPLKLWQFHVQRFCYRLYASLRIEELYSIIHDFRESKPAAPRDEQELLCGQIAGVARCALEISGSPPGLIRLRKLKFALEAEGSFLWVLGCTADLSDLSCRRLLERLLGLFRFYNGPASRAYLERPQEDLDREWELYVAHLQQSSSDLHRVFDSLWAWDKTKVDPLLLLKAALILQSCQRSRHVLAGCILYEGRVVSTQLPPELTARVLLQKGAEEGSSGPEEGASQLKEALPPGVCVTPVYLLEAEAAALRELPVEWMTRGSQEDLASSKGSETPAGGADGAPLVRMALYVHHIRGLVLALLAEEPLVSSADFMEDVYHSSLASLNGLEVHLAETLPKGPLPSSRSTYNFAHYDAVQNVLTTNVLQTTSPEDQLFLQGTGLIHATFEQLPTASELTLRNASTAVYGCRNAVQESYFQQRGLPPYNSGVPNPRDSIVGLPGKARQKLLKHGVNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSD1</accession>
    <name>A0A6I9XSD1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">STAT1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907645.1">
        <property type="nucleotide sequence ID" value="XM_014052170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSD1"/>
    <dbReference type="GeneID" id="106537889"/>
    <dbReference type="KEGG" id="tsr:106537889"/>
    <dbReference type="CTD" id="6772"/>
    <dbReference type="OrthoDB" id="19300at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000012">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.20:FF:000001">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000001">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48092">
        <property type="entry name" value="Transcription factor STAT-4 N-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="544"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="112"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013907645.1"/>
        </source>
    </evidence>
    <sequence length="621" mass="72411" checksum="597FFC4E6294CD5D" modified="2020-10-07" version="1">MEIRQYLAPWLESQDWDHAAEDFSMATLLFQNLLSQLDDQYSRFTQENNFLLQHNIRKSKRNLHNTFVEEPTYMALMISKCLQEERNILKAAEATDQMQIDSVQNSAMLSKVKELDAKVTNVKNRVIEIEQMIKNLEDVQDEYDFKFKTFQVRECESHNMTQDDYKKEKVQLHTMYLQLHNKRQDVLHLISSSLPVIENTQNALITEELVEWKHRQQMACIGGPPNACLDQLQNWFTSVAESLQQILQQLKKLEELEQKFTYDTDPITQQKKGLIERTWSLFKQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYKLKVQVQFDKDVSEKVTVKGFRKFNILGTNTKVLNMEESNGSLSAEFRHLQLKEQKNVSRSNEGPLIVTEELHSLCFETKWEQNTVVIELETHSLPIVVISNVSQLPSGWASILWYNMLMNEPKNLSFFLNPPCARWSQLSEVLSWQFSSVTKRGLSTDQLEMIGEKLLPNGCTPDGLISWARFCKENLNDKNFAFWLWIEGILELIRKHLICLWNDGHIMGFVSKEQERMLLKDMETGTFLLRFSESSRDGAITFTWVEGPANDQSFHSVEPYTKKELSAVSLPDIIRNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTH9</accession>
    <name>A0A6I9XTH9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PSMA4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914003.1">
        <property type="nucleotide sequence ID" value="XM_014058528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTH9"/>
    <dbReference type="GeneID" id="106542718"/>
    <dbReference type="KEGG" id="tsr:106542718"/>
    <dbReference type="CTD" id="5685"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000022">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="9">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013914003.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29454" checksum="148F972BF6EAB918" modified="2020-10-07" version="1">MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANNGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLQSALALAVKVLNKTMDVSKLSAEKVEIATLTRENGKTIIRVLKQKEVEVLIKKHEEEEAKAEREKKEKEQKEKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTV9</accession>
    <name>A0A6I9XTV9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">General transcription factor II-I-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106542812</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914128.1">
        <property type="nucleotide sequence ID" value="XM_014058653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTV9"/>
    <dbReference type="GeneID" id="106542812"/>
    <dbReference type="KEGG" id="tsr:106542812"/>
    <dbReference type="OrthoDB" id="10072451at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1460.10:FF:000002">
        <property type="entry name" value="General transcription factor II-I isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1460.10">
        <property type="entry name" value="GTF2I-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004212">
        <property type="entry name" value="GTF2I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036647">
        <property type="entry name" value="GTF2I-like_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46304:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46304">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02946">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117773">
        <property type="entry name" value="GTF2I-like repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51139">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="544"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013914128.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="62312" checksum="6B7EF2977A2F4FC5" modified="2020-10-07" version="1">MVDQLFCKKFAEALGSTEPKAVPYQKFEAHPTDLYVEGLPENIPFRSPSWYGIPRLEKILQVGNRIKFVIKKPELLTVSSTEVTQPRTNMPVKEDWNVRITKLRKQVEEIFNMKFAQALGLSEAVKVPYPVFESNPDNLYVEGLPEGIPFRSPTWFGIPRLERIVRGSGKIKFIVKKPELVTSFLPPELVCKVNTSVNVSVGSQSPSSSNSTRSPVSNSKVPEIEVTVDEGPANRVSTSDVRTNPQTNGSNTGFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLTEPVKVPFALFESYPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFEAAIKENATAIPNPQRRTSLCNTVINAVSVPDPVVNTAAGVEDLNIIQVTIPDDDERTSKVEKARQLREQVNDLFSRKFGEAIGMGYPVKVPYRKITVNPGCVVVDGMPPGVSFKAPSYLEISSMRKILDSAEFIKFTVIRPFPGLVMNNQITEKAEAEVPAATVPAPEQVIQEPVEADQIVQVSVEEDSVQTEEHSQIKQEPDPTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTW0</accession>
    <name>A0A6I9XTW0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein LOC106547028</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106547028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919529.1">
        <property type="nucleotide sequence ID" value="XM_014064054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTW0"/>
    <dbReference type="GeneID" id="106547028"/>
    <dbReference type="KEGG" id="tsr:106547028"/>
    <dbReference type="OrthoDB" id="9905174at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002198">
        <property type="entry name" value="Immunoglobulin heavy variable 5-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="90"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013919529.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="26694" checksum="658E911AA4E9532A" modified="2020-10-07" version="1">MYWVRQAPGKGLELLASISTGSSPSTSYSNKVKGRFTISRDDSRSQLYLQMNNLKLEDKAAYYCSTCTVRGRVQSQVQLVESGGDVRRLGESLRISCETSGFIFSNHYMYWVRKAPGKGLELLASISTSSSPSNYYPGKVKGRFTISRDDSRSQLYLQMNNLKLEDTAAYYCSTCTVRGSKVKGRFTLSRDDSRSQLYLQMNNLKLEDTTDYYCSTCTVRRSESDAEQKPSQSSSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV12</accession>
    <name>A0A6I9XV12_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SH2 domain-containing adapter protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SHB</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917927.1">
        <property type="nucleotide sequence ID" value="XM_014062452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV12"/>
    <dbReference type="GeneID" id="106545795"/>
    <dbReference type="KEGG" id="tsr:106545795"/>
    <dbReference type="CTD" id="6461"/>
    <dbReference type="OrthoDB" id="5914531at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000021">
        <property type="entry name" value="Putative SH2 domain-containing adapter protein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051846">
        <property type="entry name" value="SH2_domain_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127">
        <property type="entry name" value="HEAVYWEIGHT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127:SF31">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING ADAPTER PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="408"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="271"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="354"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917927.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="55581" checksum="50A4F053C43F606C" modified="2020-10-07" version="1">MAKWLNKYFSLGNNKTKSPPQPPRPDYRDKRNGTAGAGGGGHHNHNHNHHHHHHHHHTSPCFPRHDTSDLLRAYRAQKERDFEDPYSGPGSSLRKLRALCRPEYCAPEELLVESAGPKPFSSSSCCGGGGSGGGGGSSGGSSAAVASAPTPTASPQLFRSHSERRAATPPDSASVKYISPKHRLIKIESNEGGKGGSGVPITWSPPGIGGAGQKKLNKCSEQGEGGGGSVSLTKKEKVLIADDYSDPFDAKSEINKMGKGENTGYMEPYEAQRIMTELQRQDNVKPQQKDIQLYDTPYEPEGNCVESDSESAVSQRLRESKLPQDDDRPADEYDQPWEWNKVTIPALAAQFNGGEIRQSSPSPSSSDRRRQLRAPGGGFKPIKHGSPEFCGILGERVDPTIPLEKQIWYHGAISRTDAENLLRLCKECSYLVRNSQTSKHDYSLSLKSNQGFMHMKLAKTKEKYILGQNSPPFDSVPEVIHYYTAKKLPIKGAEHLSLLYPVAVRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVF5</accession>
    <name>A0A6I9XVF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Cell growth regulator with EF hand domain protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12 13" type="primary">CGREF1</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918070.1">
        <property type="nucleotide sequence ID" value="XM_014062595.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918071.1">
        <property type="nucleotide sequence ID" value="XM_014062596.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918072.1">
        <property type="nucleotide sequence ID" value="XM_014062597.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918074.1">
        <property type="nucleotide sequence ID" value="XM_014062599.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918075.1">
        <property type="nucleotide sequence ID" value="XM_014062600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVF5"/>
    <dbReference type="GeneID" id="106545891"/>
    <dbReference type="KEGG" id="tsr:106545891"/>
    <dbReference type="CTD" id="10669"/>
    <dbReference type="OrthoDB" id="289247at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052110">
        <property type="entry name" value="MCFD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23104:SF15">
        <property type="entry name" value="CELL GROWTH REGULATOR WITH EF HAND DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23104">
        <property type="entry name" value="MULTIPLE COAGULATION FACTOR DEFICIENCY PROTEIN 2 NEURAL STEM CELL DERIVED NEURONAL SURVIVAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636615" evidence="6">
        <location>
            <begin position="19"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013918072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013918074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013918075.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="25571" checksum="8DB9D2FBD413B368" modified="2020-10-07" version="1">MKGLFLAVWFFLVPAKWAAPKNSSGRPEIPNDSMDQPILNPLHPSQVSLKVLQDYLENIGQLNADTATMTREQALLLLFALHDYDKSGHLDGLEFMRLLNELVSQQAKGQPAPDMVVPMVDSILETQDFNWDGLLEPLELFLPPRQGETPNFLTADSEEDGQVLWALPKPNGFPSPSDQQSDGDSPPAPEVQEQDMMGDQLWSLEKHGGELGQETAGDPDQQGKEMPSAPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWV0</accession>
    <name>A0A6I9XWV0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tripartite motif-containing protein 67</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TRIM67</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915103.1">
        <property type="nucleotide sequence ID" value="XM_014059628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWV0"/>
    <dbReference type="GeneID" id="106543580"/>
    <dbReference type="KEGG" id="tsr:106543580"/>
    <dbReference type="CTD" id="440730"/>
    <dbReference type="OrthoDB" id="295536at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.40:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF21">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="232"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013915103.1"/>
        </source>
    </evidence>
    <sequence length="304" mass="32298" checksum="5A3EB595D2E92273" modified="2020-10-07" version="1">MEEELKCPVCGSLFREPIILPCSHNVCLPCARTIAVQTPESEQHLAGGSSGTGGGGGDHADKVSLHSETDSGYGSYTPSLKSPNGVRVLPLVPPPPGLSAAAALAGPRGSGTSSSLTCPQCHRSASLDPRGLRGFQRNRLLEAIVHRYQQSRAAASAMAKCQLCDRSPPEAAAVLCEQCEVLYCSACQVKCHPSRGPFAKHRLVPPPSQQQSAPSGAGGACGGGSKVLGLARKSPTCADHELENYSMYCVSCRTPVCYQCLEEGKHSKHDVKALGAMWKQHKRRVTMLSSGRKDKCHRHCKECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXR5</accession>
    <name>A0A6I9XXR5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(27) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106548216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(27)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60224"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15544"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.68"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTX family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.68" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013921029.1">
        <property type="nucleotide sequence ID" value="XM_014065554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXR5"/>
    <dbReference type="GeneID" id="106548216"/>
    <dbReference type="KEGG" id="tsr:106548216"/>
    <dbReference type="OrthoDB" id="9907521at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044666">
        <property type="term" value="C:MLL3/4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071558">
        <property type="term" value="F:histone H3K27me2/H3K27me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF5">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="164"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="269"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="660"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="938"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="328"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="550"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="566"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="598"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="624"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="678"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="717"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="744"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="786"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="835"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="845"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="940"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="953"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013921029.1"/>
        </source>
    </evidence>
    <sequence length="983" mass="105695" checksum="C5F2368583B97D33" modified="2020-10-07" version="1">GAWSSCHSRPWLPGGRCSASAVQPPLPLPLPPSHGANLGQYSKGYYAPGNLPPRPLHGKLEAIHGCVQALLRDQGQPHLWEQLGEIYESEQDCEEAIRCYQNAARYQRDYGSYGKMHAQIGRLQQAQLWNFHSGGSSHLRHKVLPPLQQVWNLLHLEKRNFSAKRNPQLKRPGPPMEPPVVQPIPPVQHPAHLGHGEEMPAPMKRRRSTSPDQNCGNGGGQHLSGSGAPGNPHYQLAKPGLWSPLHGDHWAQERKNLAVPERQVRLWPHFKAPHPPPKEGREASSNHYQTPAMESSSPTAENGGRKPPPPSRPAPCLAPRNLPCARMQPPPPNLAGPPPAAAPPPPLAPQCPPAPPPPPPLPWLNKAGEGKAEDEDPLGDILFGCGGEPCGADPPSLAHCDSGCGFQDSGPSSQQGSFSRPHEGTGDTPGPASPEPSPAPQFWLSESSREENGSGGARPRNPSPSITSGTFRSPESPPPLPVPPPPPPPLPPPSRASYPKTPEPPGVPAAMEASSDRLFGFASPPLEDQFEEPPEFSKILPDGLANIMKMLDESIRRGEEEREESFPPPPPPAALLPSPPPLARPKLPSASAGSFDYPKPPGQDPPEPPCLYRFGKRDTEEQRPPALAAAGSGGAKFAPHNTPSLLKSLANVLEGQKHSYRLKPITPPGAKISPPAGASQSPLPQFTTSGQAWAGAPGGATEERTSTPPPLPTPRPVKTESEVLEEISRACETLVGRAGREKSPPPPASPPPAPASPPPAPLPATVPPAPPPPTPPRPKDESRKPHMEHRRHRRSNRDGSRRHRDGKARKGKNRQILGSLDVQSSEGQARENGAKPPPPPPPPPPERRSPVKKEEGPVGSMSTMVCSADLLKLRSFTEGPPKELKIRLIKVESGDKETFIASEVEERRIPLSEVTINHSAAEVVRASKHAKVKGKFKESYLSQSHSVKPQINMDEKLPRDKLNPPTPSIYLESKRDAFSPVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZH9</accession>
    <name>A0A6I9XZH9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Zinc finger protein 462-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">LOC106547231</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919792.1">
        <property type="nucleotide sequence ID" value="XM_014064317.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919796.1">
        <property type="nucleotide sequence ID" value="XM_014064321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZH9"/>
    <dbReference type="GeneID" id="106547231"/>
    <dbReference type="KEGG" id="tsr:106547231"/>
    <dbReference type="OrthoDB" id="4737882at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000655">
        <property type="entry name" value="Zinc finger protein 462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001043">
        <property type="entry name" value="Zinc finger protein 462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000613">
        <property type="entry name" value="zinc finger protein 462 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF105">
        <property type="entry name" value="ZINC FINGER PROTEIN 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="419"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013919792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919796.1"/>
        </source>
    </evidence>
    <sequence length="549" mass="62708" checksum="EF37AC26ED139B89" modified="2020-10-07" version="1">MATMELAEAESGPSLEDETRPGGYHCSQCDRVLMSMQGLRSHERSHLALAMFTREDKYSCQYCSFVSAFRHNPSGDSYPLFWQSLDRHMQAHHGHHKPFRCKLCPFKSSYNSRLKTHLLKVHAGEHAYKCSSCSFSTMTISQLKEHSLKVHGKALTLPRPRIISLVTSHAQHASKNHTTAEEVEDSNDSSYSEPPDVQQQLNHYQSAALARNNNNISPVPLSGGDQKAEAILNCEFCEFTSGYIQSIRRHYRDKHGGKKLFKCKDCSFYTGFKSAFTMHVEAGHSAVPEEGPKDLRCPLCLYHTKYKRNMIDHIVLHREERVVPIEVCRSKLSKYLQGIVFRCDKCTFTCSSDECLQQHIEKHNELRPYKCQLCYYETKHTDELDAHLRDEHKVNRNFELVGRVNLDQLEQMKGKEESSSSDEEEKDEEMSPETEERGNMRFSENGPPEKRFPCEFCGRTFAEGSEWERHVLRHGMAMNETNRVISDESQAKENAEETVKVPLAEEMESSPKRLVDFSHKNEAAICVTVAATAAADNKSLPEMAEVKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1E4</accession>
    <name>A0A6I9Y1E4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">26S proteasome regulatory subunit 6B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">26S proteasome AAA-ATPase subunit RPT3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Proteasome 26S subunit ATPase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PSMC4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC4 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC4 and few additional components. Interacts with NR1I3. Interacts with PAAF1. Interacts with TRIM5. Interacts with ZFAND1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920261.1">
        <property type="nucleotide sequence ID" value="XM_014064786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1E4"/>
    <dbReference type="GeneID" id="106547565"/>
    <dbReference type="KEGG" id="tsr:106547565"/>
    <dbReference type="CTD" id="5704"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000018">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="198"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013920261.1"/>
        </source>
    </evidence>
    <sequence length="418" mass="47342" checksum="48A64B718835D252" modified="2020-10-07" version="1">MEEIGISVEKTQDELPALSISRPQTGLSFLAPEAEDVEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQEAGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1Q9</accession>
    <name>A0A6I9Y1Q9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Histidine triad nucleotide-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">HINT1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine 5'-phosphoramidate + H2O = NH4(+) + AMP</text>
            <dbReference type="Rhea" id="RHEA:67916"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57890"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HINT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920406.1">
        <property type="nucleotide sequence ID" value="XM_014064931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1Q9"/>
    <dbReference type="GeneID" id="106547690"/>
    <dbReference type="KEGG" id="tsr:106547690"/>
    <dbReference type="CTD" id="3094"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="18"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013920406.1"/>
        </source>
    </evidence>
    <sequence length="76" mass="8215" checksum="A57D2B536E8396F0" modified="2020-10-07" version="1">MADEVCRAQAAQPGGDTIFGKIIRKEIPAKIIFEDEKCLAFHDISPQAPTHFLVIPKKHIAQISQVEDADAGSSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2X3</accession>
    <name>A0A6I9Y2X3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cytochrome P450 2J5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106540566</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911210.1">
        <property type="nucleotide sequence ID" value="XM_014055735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2X3"/>
    <dbReference type="GeneID" id="106540566"/>
    <dbReference type="KEGG" id="tsr:106540566"/>
    <dbReference type="OrthoDB" id="3934656at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF327">
        <property type="entry name" value="CYTOCHROME P450 2F2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027109479" evidence="13">
        <location>
            <begin position="26"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013911210.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="43548" checksum="7F0DDDA929F88C58" modified="2020-10-07" version="1">MTAFGWLIVLSLLFLWFWMNQRPKGFPPGPWKLPLLGNLLYFSQREPLKKLDQLGKKYGPIFSLYLGRTPVVLTYGFPSAKEVLMVQGIEFAGRASFPIMDAITSKKGILTLRYGEIWKEQRRFSLMLLRNFGMGKKSMEEKILEEASYLIQVFTENISMPFGPYEFVDSAVINVISTILFGKRFEYDNMFLRTLLDDIHQSSKMTTGPWGLLYNEMPLVRSLPLPHQTILATGKKVDVFIKKEVAEHKATMTPGEPRDFIDAYLEEMQKPERSGSSFEEGQLEILLSDHLVAGAETTSGTLQWGLLYLMAFPEIQGPRVCLGENLARMELFFFFTSILRNFQLLWPDKSQAPDFTPHFAITQFPSHFKMLLKCRQSCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3E2</accession>
    <name>A0A6I9Y3E2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sterol 26-hydroxylase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106540772</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911495.1">
        <property type="nucleotide sequence ID" value="XM_014056020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3E2"/>
    <dbReference type="GeneID" id="106540772"/>
    <dbReference type="KEGG" id="tsr:106540772"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042359">
        <property type="term" value="P:vitamin D metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF123">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 27 SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013911495.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="60069" checksum="6C6C987A5D7B53CF" modified="2020-10-07" version="1">MVKWSSFAGRLGRCLQSPPGVFRPAPLWRATSSAAEAASVPRHEERLKGPEELPGMGSLETLYWHFVRGYLLHTHQLQIISKKRYGPMWKSIIGPYLNINIGSPEILEELLRQEGKYPIRSDMSLWKEHRDIRHLSYGPFTEDGEKWHHLRQVLNKRMLKPSEAMLYADSINEVVSDLIVHLEDERSKSSSGVKVEDMANLLYRFALEGISYILFETRIGCLEKQMPVETKNFIDAIGYMFRNSVFVTFLPKWTRNMLPFFNRYLQGWDTIFGFGKKLIDQKTLDLEKRLERGEEVSGYLTYLLTSGRLSNEEIYGSITELLLAGVDTTSNTMSWCLYHLARNPDIQEALYQEIANVVPADQVAEAKDINKMPLLKAIIKETLRLYPVVPTNARIIAEKEVVIQGYRFPKDSLFVLAHYGMSHDESNFPEPERFLPRRWLRDQREVSPHPFSSIPFGYGIRACAGRRIAELEMHLALIRIMQKFLIRPDPQCTDVKPLSRIVLVPDKPINLDFLDRQTMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3N2</accession>
    <name>A0A6I9Y3N2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription initiation protein SPT3 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SUPT3H</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918073.1">
        <property type="nucleotide sequence ID" value="XM_014062598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3N2"/>
    <dbReference type="GeneID" id="106545889"/>
    <dbReference type="KEGG" id="tsr:106545889"/>
    <dbReference type="CTD" id="8464"/>
    <dbReference type="OrthoDB" id="66982at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918073.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="26106" checksum="320762DCEAD03B6E" modified="2020-10-07" version="1">MSSTGPSPMSAVPASSGRGSGRSTNFILELQSMMFSLGDARRPLHESAILVEDIVHTQLINLLQQASEVSQMRGARVISAEDLIFLMRKDKKKLRRLLKYMFFRDYKSKVVKGIDEDDLLEDKFSSSTNKRQKTAQDFLTSIDQTGELLALFEDDEIDDVKQERMERAERQARVMDSAQYAEFSESRQLSFCKYCVKFLLSKVVFFKLVVVKKQIHLMPKQGKNVIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y412</accession>
    <name>A0A6I9Y412_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Histone acetyltransferase</fullName>
            <ecNumber evidence="4 14">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">KAT2A</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4 14"/>
    <dbReference type="RefSeq" id="XP_013911990.1">
        <property type="nucleotide sequence ID" value="XM_014056515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y412"/>
    <dbReference type="GeneID" id="106541157"/>
    <dbReference type="KEGG" id="tsr:106541157"/>
    <dbReference type="CTD" id="2648"/>
    <dbReference type="OrthoDB" id="1937912at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000014">
        <property type="entry name" value="Histone acetyltransferase KAT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016376">
        <property type="entry name" value="GCN5/PCAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009464">
        <property type="entry name" value="PCAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF1">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06466">
        <property type="entry name" value="PCAF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003048">
        <property type="entry name" value="Histone_acetylase_PCAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 17" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="20">
        <location>
            <begin position="488"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="19">
        <location>
            <begin position="730"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="25"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="15">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="564"/>
            <end position="566"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="571"/>
            <end position="577"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="602"/>
            <end position="605"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR003048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR003048-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR003048-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013911990.1"/>
        </source>
    </evidence>
    <sequence length="822" mass="93458" checksum="F98F704AB6DB3FF5" modified="2020-10-07" version="1">MLSGSHAPTPSCRVLGIMAEAEVSQTTRIPQTSAPSSATSSAVTTPPVVGGSGAGGSTSDPVRPGLSQQQRASQRKAHARSFPRAKKLEKLGVFSACKANDSCKCNGWKNPNPPTAPRMDLQQTVTNLSEPCRSCGHTLADHVSHLENVSEEEINRLLGMVVDVENLFMSVHKEEDTDTKQVYFYLFKLLRKCILQMSRPVVEGSLGSPPFEKPNIEQGVLNFVQYKFSHLPPKERQTMFELSKMFLLCLNYWKLETPSQFRQRSQNDDVATYKVNYTRWLCYCHVPQSCDSLPRYETTQVFGRNLLKSTFTVTRRQLLEKFRVEKDKLVPEKRTLILTHFPKFLSMLEEEIYGESSPIWEADFTMPATEGAPLVPRPAISTVPAASTPLFNKSLSCSSSLTSINLDGSSLDSVPGEKRKLPESLTLEDAKRIRVLGDIPMELVNEVMLTITDPAAMLGPETSLLSANAARDETARLEERRGIIEFHVIGNSLSQKSNKKILMWLVGLQNVFSHQLPRMPKEYITRLVFDPKHKTLALIKDGRVIGGICFRMFPTQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHSILYFLTYADEYAIGYFKKQGFSKDIKVPKSRYLGYIKDYEGATLMECELNPRIPYTELSHIIKKQKEIIKKLIERKQAQIRKVYPGLTCFKEGVRHIPVESIPGIRETGWKPLNKEKGKELKDPDQLYTTLKNLLAQIKTHPSAWPFMEPVKKSEAPDYYEIIRFPIDLKTMTERLKNRYYITKKLFIADLQRVITNCREYNPPESDYCKCANTLEKFFYFKLKEGGLIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4W4</accession>
    <name>A0A6I9Y4W4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 24 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCDC24</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919288.1">
        <property type="nucleotide sequence ID" value="XM_014063813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4W4"/>
    <dbReference type="GeneID" id="106546839"/>
    <dbReference type="KEGG" id="tsr:106546839"/>
    <dbReference type="CTD" id="149473"/>
    <dbReference type="OrthoDB" id="6022633at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031367">
        <property type="entry name" value="CCDC24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28601">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28601:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15669">
        <property type="entry name" value="CCDC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919288.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="43535" checksum="3D3733BAD5E046A7" modified="2020-10-07" version="1">MLELEIMENSFSWEPLKPVPSLWRMIEEQLAPSERLEVKTILGIDLVDHSLELHDEVKTLLEFYQESQLDHFEQRPDNCILLAAPPHLKELVREEIRLLLVGLQQKALQEGRDHDCAIAKYNTNVINFAFKTNAGSNRPSSRSNNLIRSLSCSSPNDLEPYSDKLNITQIGEIASRLRTLLEEECHALERYIILLQNQLEEVHQHATEMQDKMHEPTMAELQEEKRVMEWDLQLSLPKSCPRTPSPMSNQFWNSTNLSQLSHLRGLVKGPGFGQINPASNEIPSPDPRDQISLLCYQPSSRWSPSTRTPLEWMHQKSEDSNQARKDSDTKLPTTLGHPTASGYRPASASAWEPAFLPMPPAEPCPPTRFCPRIRLLQCKGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y542</accession>
    <name>A0A6I9Y542_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine 3-monooxygenase</fullName>
            <ecNumber evidence="5">1.14.16.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Tyrosine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TH</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013919488.1">
        <property type="nucleotide sequence ID" value="XM_014064013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y542"/>
    <dbReference type="GeneID" id="106546997"/>
    <dbReference type="KEGG" id="tsr:106546997"/>
    <dbReference type="CTD" id="7054"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="UniPathway" id="UPA00747">
        <property type="reaction ID" value="UER00733"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004511">
        <property type="term" value="F:tyrosine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006585">
        <property type="term" value="P:dopamine biosynthetic process from tyrosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03345">
        <property type="entry name" value="eu_TyrOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000002">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041903">
        <property type="entry name" value="Eu_TyrOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021164">
        <property type="entry name" value="Tyrosine_hydroxylase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF18">
        <property type="entry name" value="TYROSINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12549">
        <property type="entry name" value="TOH_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0408">Iron</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="17">
        <location>
            <begin position="103"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013919488.1"/>
        </source>
    </evidence>
    <sequence length="449" mass="50999" checksum="3F4833B950D9EAE2" modified="2020-10-07" version="1">MPTPNASSSSAKGFRRAVSELDSKQAEAIMSPQFIGRRQSLIEDARKEREAAAAAASEAAELTETIVFDEKDGIAVLNLLFTLKGGKTSLPSRALKIFEIKFNIPNITNSIMSLFHWFPRKISELDLCHHLVTKFDPDLDLDHPGFSDEVYRERRRMIAEIAFHYKHGDPIPRVEYTEEEITTWREVYSTLKSLYPTHACKEHLDAFHLLERYSGYSENNIPQLEDVSCFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADKTFAQFSQDIGLASLGATDEEIEKLSTLYWFTVEFGLCKQNGIIKAYGAGLLSSYGELIHSLSDEPELRDFDPDVTAMQPYQDQTYQPVYFVSESFNDAKAKLRVYAAHIKRPFSVKYDPYTYSIELLDHPQKICHSLANVRDELHSLIDALNIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6E2</accession>
    <name>A0A6I9Y6E2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein patched homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PTCH2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920593.1">
        <property type="nucleotide sequence ID" value="XM_014065118.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547853"/>
    <dbReference type="KEGG" id="tsr:106547853"/>
    <dbReference type="CTD" id="8643"/>
    <dbReference type="OrthoDB" id="5873834at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097108">
        <property type="term" value="F:hedgehog family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008158">
        <property type="term" value="F:hedgehog receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005119">
        <property type="term" value="F:smoothened binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000003">
        <property type="entry name" value="protein patched homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000007">
        <property type="entry name" value="Protein patched homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004766">
        <property type="entry name" value="TM_rcpt_patched"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00918">
        <property type="entry name" value="2A060602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46022">
        <property type="entry name" value="PROTEIN PATCHED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46022:SF3">
        <property type="entry name" value="PROTEIN PATCHED HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="935"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="962"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="991"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1030"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1056"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="10">
        <location>
            <begin position="347"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1276"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1281"/>
            <end position="1296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013920593.1"/>
        </source>
    </evidence>
    <sequence length="1312" mass="145108" checksum="21969CD4FE2A2B94" modified="2020-10-07" version="1">MNPRSMDGSNREQDLFVCMEDYVTPDLWHEGYSPETHLPSFEGSRVSQELRYTREKLGEESLYTSQMLIQTPKQEGENILTPDALEMHLEAALAASKVQVLLFGKSWDLNKICYKSGVPIIENGMIARMIEKLFPCVVVTPLDCFWDGAKLQGGSAYLPGRRDIQWTNLDPIQLMEELGQFASLEGFKEMLDKAEVGQAYMERPCLDPFDPECPSSAPNSQSQKKPHIPSELTGGCHGFSRKYMHWQEELILGGTVKDPQGKLQKAEALQSMFLLMSPRQLYEHYRDDYEIHDISWSEEKAAAVLEAWQREFVEVAQESLPSNYSQNIHAFSTTTLSDMMKSFSDVSAIRVAGGYLLMLAYACVTLLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLLGISFNAATTQVLPFLALGIGVDDMFLLAHAFTETSQHVPVKERTGECLRRSGTSVALTSVNNMIAFFMAALVPIPALRAFSLQAAVVVVFNFAMVLFIFPAILSLDLHRRENKRLDILCCFYSLCSARVIQIQPQEYADVNDNHTAPTYPHGHPALATSTHITTTVQAFTQCDSSGRHVVTILPPTQHVFTNPSTLLPANPLGSQIFTPASSTRDLLAQLDESKGAQECVPLPICRWNLSSFARDRYAPILLQSQTKGIVVALFLALLGLSLYGTTKVHDGLYLTDIVPRGTKEHAFISAQFKYFSFYNMFVVTNGGFDYPNSQEALYSLHKTFAAIQYVVREENRQLPKMWLHYFRDWLQDLQAAFNQDWKAGHITRENYRNGSEDGVLAYKLLIQTGNKKEPFNLNQLTTRRLVDENGIIPPDIFYICLTVWVSNDPLGFAASQANFYPPPPEWIHDKYDATGENLRIPAAQPLEFAQFPFYLNGLQHTSDFVAAIQSVRAICDEMAQTHGVFSYPSGYPFLFWEQYIGLRHWLLQAISIVLACTFLVCTLLLLNPWTASIIVFVLAMMTVELFGIMGLMGIKLSAIPVVILIASVGIGVEFTVHVALGFLTAIGSRNLRSTVALEHMFAPVMDGAVSTLLGVLMLAGSEFDFILRYFFAVLTILTILGLLNGLVLLPVLLSIIGPPAEVTPADNGSHLPTPSPVPPPINHHSFYIRQNGNAFGDSSDSEYYSETVSASETGEDDRSTYIISPAQSQILVKASKSPNFPQITVVKSYKTSLESPLSLCSPEWTQSRTPGGYLPAAVTEIKSCLQQQEQPNHRLGSQLAQSTPNPLQTNPITHSTKIPPSGHGGTFTTVTATASVMLALHPPLPGSCRGYTHEGFESDDNSEKPGPKPSDSLELQDLKQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y741</accession>
    <name>A0A6I9Y741_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Msx2-interacting protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106543156</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914550.1">
        <property type="nucleotide sequence ID" value="XM_014059075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y741"/>
    <dbReference type="GeneID" id="106543156"/>
    <dbReference type="KEGG" id="tsr:106543156"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="msx2-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="379"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="273"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="85"/>
            <end position="100"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="130"/>
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        <location>
            <begin position="146"/>
            <end position="179"/>
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            <end position="285"/>
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        <location>
            <begin position="293"/>
            <end position="340"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013914550.1"/>
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    </evidence>
    <sequence length="466" mass="53733" checksum="66522B951C1CDFCD" modified="2020-10-07" version="1">MVRENKHLWVGNLPENVREEKIIKHFKRASDNRERAYEPSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYSSRSRSPSRFDAHDPHYKPRAREQFTLPSVVHRDIYRDDLTREVRGRRSERSYQHSRSRSPHSFQSRTQSPQRLASQASRSSRSPSGSGSRSQSSSSNSVRNSNDRASDNRERAYEPSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYSSRSRSLSRFDVHGHHYEPRAREQFTLPSVVHRDIYRDDLTREVRGRRPERSYQHSRSRSPHSSQSRTQSPQRLASQASRSTRSPSGSGSRSRSSSSDPIRSSSNTSRDSSDSSDESSARSVQSTAVPAPTSQLLPPLEKDEPRKSFGIKVQNLPVRSTDTSLKDGLFHKFKKYGKVTCVQIHGASEERYGLVFFRQQEDQEKALNASKGKLFFGIGPQDGVPVVTIKQSKMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7D1</accession>
    <name>A0A6I9Y7D1_9SAUR</name>
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        <submittedName>
            <fullName evidence="7">Calcyphosin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CAPS2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_014059490.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Ca-bind"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
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        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CALCYPHOSIN-2"/>
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        <property type="entry name" value="EF_HAND_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="412"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="16"/>
            <end position="84"/>
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            <begin position="18"/>
            <end position="29"/>
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            <begin position="72"/>
            <end position="84"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013914965.1"/>
        </source>
    </evidence>
    <sequence length="581" mass="67445" checksum="E79600884E1D9627" modified="2020-10-07" version="1">MDLEVKGIAASPKRPVLLSSQGWRPNTRLSHGPRPADVPCLDLGKLGDSEDEDGDNGSLYNGVRPYTGPPRVSSSFRRGTQSTHTQYHNIEQAARRHDLKKIELSSKEQKTIPENLPLPTDIYKLKYQQYETEMKEGYKQYSQRAAKKKINNNLFHELPCKIAEKKDLPPEKQRVDEFTVLDEKALLQQGYTNKPYTMQHSIRKFDVEEVAAERKKQAVVEQVMLDQLCRAVISDPEQNGNSDIYKMYPVLPGLSTAPLRFRKRTLHETKIKTRSALTENMLSNKLRFDGRLILRNGRDACRELIGFYFTCDKSLTIYEYRKFGKNRTNALPFILKGIYMHQRGQRKGKPYRLNDFFVVSILSPDRRKVILFTYLKTITVFKRKKIINNFNQPFKISSLDNLRTKLSKRGVRIVSGLGKYFRELDKKQNGNITKADYRQALKVFHLEMTEEDFESLWLVLHDDNNGELDYTEFIRAILGEMDEYRKAFVRKAYMKLDYNKIGRIPMVDIKKCYCARKHPQVIAGYATEEEIKSSFLETLEDACSNPSEVSFGEFEDYYEGLSIGIPDDEDFINILRNPWGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7I6</accession>
    <name>A0A6I9Y7I6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 21">5-hydroxytryptamine receptor 2A</fullName>
            <shortName evidence="21">5-HT-2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 21">Serotonin receptor 2A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">HTR2A</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">G-protein coupled receptor for 5-hydroxytryptamine (serotonin).</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts (via C-terminus) with MPDZ and PATJ. May interact (via C-terminus) with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Cell membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Cell projection</location>
            <location evidence="1 21">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cell projection</location>
            <location evidence="21">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 21">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 21">Membrane</location>
            <location evidence="2 21">Caveola</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915240.1">
        <property type="nucleotide sequence ID" value="XM_014059765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7I6"/>
    <dbReference type="GeneID" id="106543701"/>
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    <dbReference type="OrthoDB" id="420518at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007208">
        <property type="term" value="P:phospholipase C-activating serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007210">
        <property type="term" value="P:serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000455">
        <property type="entry name" value="5HT2A_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF30">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00516">
        <property type="entry name" value="5HT2ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0085">Behavior</keyword>
    <keyword evidence="5 21" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17 21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 20" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 20 24" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 21" id="KW-0770">Synapse</keyword>
    <keyword evidence="15 20" id="KW-0807">Transducer</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="22">
        <location>
            <begin position="88"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU368060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013915240.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="52523" checksum="4F5A3B40D72F9676" modified="2020-10-07" version="1">MDIFSEEWSVNLIAHSLLELSQARKFNRSVLTSEEINKSCLCNVTADFDHTTNCPCEGNLTTSLFPILHEKNWPALLTIIVIIITIFGNILVILAVSLEKKLHNATNYFLMSLAITDMLLGFLVMPISMLTILYEYRWPLPRNLCTVWIYFDVLFSTASIMHLCAISLDRYIAIQDPIHHSRFNSRTKALAKIALVWTVSIGISMPVLIFGFHDNSKVFKNGSCFLVEEKFVLIGSFVAFFIPLVIMVITYFLTIKSLQKEATSCMNDLDSKPGSFKFFPQSSLSLDKHFTHSLNKESGTFKRRKMQSISNEQKASKVLGIVFFLFVVMWCPFFITNVMIAFCKEACYKDVIEDLLNIFVWIGYLSSAVNPLIYTLFSKTYRSAFVRYLQCHYKEKKKPLQSFLVNTIPTVVYNSHQLTQMKCLRKESKLMATGTSEIHDLDETLKSKVSNMNEKVSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7P1</accession>
    <name>A0A6I9Y7P1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Acyl-coenzyme A thioesterase THEM4</fullName>
            <ecNumber evidence="19">3.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Thioesterase superfamily member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC106543935</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40151"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40152"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:40140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>decanoyl-CoA + H2O = decanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40059"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27689"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:40060"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>dodecanoyl-CoA + H2O = dodecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:30136"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>hexadecanoyl-CoA + H2O = hexadecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16645"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="3.1.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:16646"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>octanoyl-CoA + H2O = octanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:30144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>tetradecanoyl-CoA + H2O = tetradecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:40120"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the THEM4/THEM5 thioesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.2" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013915515.1">
        <property type="nucleotide sequence ID" value="XM_014060040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7P1"/>
    <dbReference type="GeneID" id="106543935"/>
    <dbReference type="KEGG" id="tsr:106543935"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052365">
        <property type="entry name" value="THEM4/THEM5_acyl-CoA_thioest"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12418">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE THEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12418:SF19">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE THEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thioesterase" evidence="27">
        <location>
            <begin position="102"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="29">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013915515.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="19044" checksum="35846381618127A2" modified="2020-10-07" version="1">MMDQFEKFTKMSEDGSWQHLTSYENGLAASFFFRRFFFFSPPPFVVRSKSGYTPRKHQDACLFVRNVATEGRGFEFAMFYNDSERRMMVLLQPGSYLEGMIGFVHGGAVAAILDHTFASCGICAFGIVLTANLSVNYKSLIPLGSVVLVETKVEKVEEKKIFLSGCVQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7T1</accession>
    <name>A0A6I9Y7T1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transient receptor potential cation channel subfamily M member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106544087</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915715.1">
        <property type="nucleotide sequence ID" value="XM_014060240.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106544087"/>
    <dbReference type="KEGG" id="tsr:106544087"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1010">
        <property type="entry name" value="TRPM, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
        <property type="entry name" value="TRPM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041491">
        <property type="entry name" value="TRPM_SLOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032415">
        <property type="entry name" value="TRPM_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037162">
        <property type="entry name" value="TRPM_tetra_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF13">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY M MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18139">
        <property type="entry name" value="LSDAT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25508">
        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16519">
        <property type="entry name" value="TRPM_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="645"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="675"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="714"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM SLOG" evidence="13">
        <location>
            <begin position="133"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM-like" evidence="14">
        <location>
            <begin position="328"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="11">
        <location>
            <begin position="583"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM-like" evidence="14">
        <location>
            <begin position="805"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM tetramerisation" evidence="12">
        <location>
            <begin position="888"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="523"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1026"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1217"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1038"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1247"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1270"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1284"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013915715.1"/>
        </source>
    </evidence>
    <sequence length="1299" mass="146801" checksum="5CD608A5241A545E" modified="2020-10-07" version="1">MEAKGLGTKKGPGGKLSGSVRRMSSSFQRTSLKGSISGSQKGQKAWIEKTFFKRECCRIIVNSKDPTRCCCGQLLLQHIPIPVEAASKDGEDATKQSEAQPEKWSISKHTHALPTDAFGNLEFQGGGHINKSMYIRVSYDTKPDLLLQLMVKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGIIRHVGDALKDHSSKSRGRICAVGIAPWGIVENKEDLLGKDVSLAPHFWGVFNQGAVEASLMPLLTPNHIINESLRDQLLVTIQKTFNYSRSQAHQLFVILMECMKKKELAKALKLLGMEDDEPPTKGKKKKKKKKEEDIDIDVDDPEVSRFQYPFHELMVWAVLMKRQKMALFLWQRGEEAMAKALVACKLYKAMAHECMESELVDDISQDLDNNSKDFGQLAVELLDQSYKHDEQVAMKLLTYQLKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLHMRKNPGLQVIMGILLPPTILLLEFRTNDEYQTSGEKEVGKEKEEENADANADAGSRKGDEEGGKTKQKGLPIGTKIYEFYNTPIVKFWFYTICYLGYLMLFNYIILVRMERWPSIQEWVVISYILTLALEKVREILMSEPGKLSQKIKVWLQEYWNITDLVAISVFLIGAVLRLQNPPYMSYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPDEEPTWRLARNIFYMPYWMIYGEVFADQIDLYAMEINRKLINESKAVRRPHPLTPLGRRFLPLLHQVNALEQSMLDALVLDRVDFVKLLIENGVNMQRFLTIPRLEELYNTVSDTRKFLVKPCFKTTLLAMDMSHQVEFGVGQSNKPPGGVENMSMRLEEVNEREHFMKASLQTVDLRLSQLEDTSGRMATALEKLAGIDTSELILPRSRASSECDAAYLLRQSSVNSSDGYSIYRYQADELIGDTLPTPMSPALALREADPKMQLSPERQGSLLSALGPNAAASMESKNSLEAVRAAPSPYSSSVNPQDQSPSYESVSQSLMRMDSIEKRRSGTQLLLQKAQLPLGKTKLEATTSYPLDGPKARRYYPTETGYASHVALMKSKSFILAQGRELVGGYNNWTVMDQEYRTIMNQVCPSAVQPWTAEWKYELQQKMDDEPPPKYPGIVPQLEGQVEQKCLMASPEEDSEIGKVEGLSLSPVSSPLIQRRPSCNRHSVKSGRAGKYPPGRSRSLHSRSQRTKSIKERLNRPGHASSISSLMIPSGSPTEEWKTNQEKPSTETEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8A5</accession>
    <name>A0A6I9Y8A5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Delta(24)-sterol reductase</fullName>
            <ecNumber evidence="6">1.3.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">24-dehydrocholesterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">3-beta-hydroxysterol Delta-24-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">DHCR24</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="25">Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>5alpha-cholest-8-en-3beta-ol + NADP(+) = zymosterol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16608"/>
            <dbReference type="ChEBI" id="CHEBI:18252"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.72"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:36401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>lanosterol + NADPH + H(+) = 24,25-dihydrolanosterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:33919"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:28113"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:33920"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Steroid biosynthesis; cholesterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.72" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013916585.1">
        <property type="nucleotide sequence ID" value="XM_014061110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8A5"/>
    <dbReference type="GeneID" id="106544743"/>
    <dbReference type="KEGG" id="tsr:106544743"/>
    <dbReference type="CTD" id="1718"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050614">
        <property type="term" value="F:Delta24-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000032">
        <property type="entry name" value="Delta(24)-sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040165">
        <property type="entry name" value="Diminuto-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801">
        <property type="entry name" value="24-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801:SF0">
        <property type="entry name" value="DELTA(24)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0521">NADP</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="22" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="21" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="11 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 28" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="29">
        <location>
            <begin position="60"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00056986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00078485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00080612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013916585.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="60193" checksum="E9DDE7273CD93CB3" modified="2020-10-07" version="1">MGPLLSLGIGLLLFLLWARYRGIEYVLVHHRWIFVCLFLLPLSVLFDIYYQLRAWVVQKLHCAPRQHDLRVRNIQKQVREWKAEGQKTYMCTGRPGWLTMSLRIGKYKKTLKNITINLMDILNVDTERQVVRVEPLVSMGQLTAHLNRMGWTIPVVPELDDLTVGGLLMGTGIESSSHIYGLFQHICVACELVLADGSYVRCTPDENSDLFYAVPWSCGTLGFLVAAEIKMIPSKNYVKLHYEPVRGLKAICKRFAEESEKKENHFVEGIVYSSEEAVIMTGVLTNEAEQEKVNRIGTYYKPWFFKHVENYLKANTTGTEYIPARDYYHRHTRSIFWELQDIIPFGNHPIFRYLFGWMVPPKISLLKLTQGESMRKLYEQHHVIQDMMIPMKSLEQCIQTFHSVIKVYPLWLCPFILPHNPGMVHPKGDEEELYVDVGAYGMPQTKHFEAMASTRQLEKFVRNVHGFQMLYADCYMTREEFWEMFDGSLYHKLRQELQCNKAFPEVYDKVCKAARH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y921</accession>
    <name>A0A6I9Y921_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C-type lectin domain family 5 member A-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106545782</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917915.1">
        <property type="nucleotide sequence ID" value="XM_014062440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y921"/>
    <dbReference type="GeneID" id="106545782"/>
    <dbReference type="KEGG" id="tsr:106545782"/>
    <dbReference type="OrthoDB" id="7357196at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052869">
        <property type="entry name" value="CLEC5A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47536">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 5 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47536:SF1">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 5 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="6">
        <location>
            <begin position="77"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917915.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="21794" checksum="D61BEAA07CAA2BA2" modified="2020-10-07" version="1">MNWQQVAPGVILLLVKLTGTSLFFVFFPQIFPKGNITSISTENSSVWPPSPEQTSSFSPLYPATVRTPLKQPRWERFRGHDYLFSEEKNTWQISQLECEDWTSKLLIIDDTKELEFIINKTAMANYFIGLTYSEPIKKWKWIDHKDHNMILFPIKATMDKECAAILAGNVSPVSCNDEYNWICEKTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9D7</accession>
    <name>A0A6I9Y9D7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane-spanning 4-domains subfamily A member 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106546214</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918495.1">
        <property type="nucleotide sequence ID" value="XM_014063020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9D7"/>
    <dbReference type="GeneID" id="106546214"/>
    <dbReference type="KEGG" id="tsr:106546214"/>
    <dbReference type="OrthoDB" id="8951938at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF155">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013918495.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="22943" checksum="9939338B0A3D5449" modified="2020-10-07" version="1">MQGMGNFRLGRRTVMYTVETIPKGKNRVLGTIQIMTGFIHISFGIILSTLSSVYTSFFVYGEIPFLGGASFIVSGCLSIGAEKSPTECAVKGSQAMNIISAIFALLGILTFIVDLSINGPFHSDDNYEYSLVMTGNRIAIVLLTFTIVEFCIASATAYFWCRATRSDSNEAILIDPNTVRTDNEVPSVESSMHYPPSSNDVSYDLKVEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA17</accession>
    <name>A0A6I9YA17_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MyoD family inhibitor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106547117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDFI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919645.1">
        <property type="nucleotide sequence ID" value="XM_014064170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA17"/>
    <dbReference type="GeneID" id="106547117"/>
    <dbReference type="KEGG" id="tsr:106547117"/>
    <dbReference type="OrthoDB" id="8958154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026134">
        <property type="entry name" value="MDFI/MDFIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304:SF1">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15316">
        <property type="entry name" value="MDFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919645.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="29894" checksum="C9A639C5B86758A0" modified="2020-10-07" version="1">MSQVRSDLSSRSTDLPDGDPQGDSKLIPAPSTGQDTNSCPSSRPTSEVASATSHSVTSTSSSQNQTTVVKDEISLEERSTSHRSLMNGNSSSNTESEGNFTAAEAVTCQPQSRVTVQIPSDLTCIPLLQNGAGYLSDDLSNSKGETGNGILCSELRSLSSSQKPHRKLQSHHSINSHISKKSKSSSKSASSQIPTEAQEDCCVHCILSCLFCEFLTLCNIMLDCATCGSCSSEDSCICCCCCGSGECADCDLPCDMDCGIIDACCESADCLEICMECCGLCFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDI6</accession>
    <name>A0A6I9YDI6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 25 member 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLC25A43</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922217.1">
        <property type="nucleotide sequence ID" value="XM_014066742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDI6"/>
    <dbReference type="GeneID" id="106549167"/>
    <dbReference type="KEGG" id="tsr:106549167"/>
    <dbReference type="CTD" id="203427"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="77"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013922217.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="21585" checksum="6144BAFA05AC67FD" modified="2020-10-07" version="1">MVRHGHLEARRTADCAAAVGLRRAGRGGEPQLDGPAGGAYRAGPGRGSARAEKRPLASRPQPVPGRGTGRALERQPDRLLAPPALQRRAARLLPPAQSSHLPCSGGVDVRFSGVIDCFKQTVKTKGVLALWNGLTANLLRIVPYFGIMFSTFEFCKRVCLYWNGYTDSPLRYALTPGVDQSLRPQELQDVKLLGRRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDJ4</accession>
    <name>A0A6I9YDJ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Receptor-type tyrosine-protein phosphatase kappa-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106549175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922227.1">
        <property type="nucleotide sequence ID" value="XM_014066752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDJ4"/>
    <dbReference type="GeneID" id="106549175"/>
    <dbReference type="KEGG" id="tsr:106549175"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000006">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase T isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF8">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027116949" evidence="3">
        <location>
            <begin position="24"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="4">
        <location>
            <begin position="35"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013922227.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21006" checksum="2898D8CAE3D8C858" modified="2020-10-07" version="1">MAPEAGALGLLAAIVSFVAPSAAFLGVARGQFSAGGCTFEDGPGPCDYHQDPYDDFDWVHVSAQEPHYLPPEMPLGSYMIVDSSHHSHGEKARLQLPTMKENDTHCIDFSYLLYSKKGTSPGTLNILVRVNKGPLANPIWNVTGSTGKDWLRAELAVSTFWPNEYQVMKSDEKCLYPRPACKFVHLLAMHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFT7</accession>
    <name>A0A6I9YFT7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytokine receptor common subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">IL2RG</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922864.1">
        <property type="nucleotide sequence ID" value="XM_014067389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFT7"/>
    <dbReference type="GeneID" id="106549683"/>
    <dbReference type="KEGG" id="tsr:106549683"/>
    <dbReference type="CTD" id="3561"/>
    <dbReference type="OrthoDB" id="8942047at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922864.1"/>
        </source>
    </evidence>
    <sequence length="98" mass="10797" checksum="389A9E8F18B7488F" modified="2020-10-07" version="1">MERVWVVLMPRIPNPSKKFEDLFTAYQGNFSEWAGVPKDVVETFKPIYYENICAITELLPSGGYLSESSEPPAKCGEPSDVLPDPPPKAKPGPAEVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFY9</accession>
    <name>A0A6I9YFY9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylcytosine dioxygenase TET</fullName>
            <ecNumber evidence="1">1.14.11.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106549946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming during embryonic development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5-formyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-carboxyl-2'-deoxycytidine in DNA + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53832"/>
            <dbReference type="Rhea" id="RHEA-COMP:13656"/>
            <dbReference type="Rhea" id="RHEA-COMP:13657"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:137731"/>
            <dbReference type="ChEBI" id="CHEBI:137732"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5-hydroxymethyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-formyl-2'-deoxycytidine in DNA + succinate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:53828"/>
            <dbReference type="Rhea" id="RHEA-COMP:13315"/>
            <dbReference type="Rhea" id="RHEA-COMP:13656"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:136731"/>
            <dbReference type="ChEBI" id="CHEBI:137731"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5-methyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-hydroxymethyl-2'-deoxycytidine in DNA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:52636"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="Rhea" id="RHEA-COMP:13315"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="ChEBI" id="CHEBI:136731"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">The zinc ions have a structural role.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TET family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.80" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013923183.1">
        <property type="nucleotide sequence ID" value="XM_014067708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFY9"/>
    <dbReference type="GeneID" id="106549946"/>
    <dbReference type="KEGG" id="tsr:106549946"/>
    <dbReference type="OrthoDB" id="8854879at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070579">
        <property type="term" value="F:DNA 5-methylcytosine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141166">
        <property type="term" value="P:chromosomal 5-methylcytosine DNA demethylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030099">
        <property type="term" value="P:myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040175">
        <property type="entry name" value="TET1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23358">
        <property type="entry name" value="METHYLCYTOSINE DIOXYGENASE TET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23358:SF3">
        <property type="entry name" value="METHYLCYTOSINE DIOXYGENASE TET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="533"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013923183.1"/>
        </source>
    </evidence>
    <sequence length="1104" mass="122855" checksum="5B1658DEB69B739D" modified="2020-10-07" version="1">MEQDRTNHDEGNSLNPFLIPHPSRVCQAEPLSVKLHNGNTSVGKIYSELNGNPMWLHGKANYGMKHPATRNESSRISPDLLQQKKHCSGYLQNGGIKRTFSEPSLYGLHLNKKLKQDEEAKRGQDGACAVSNQAGASNLCSEKDVVGFGTEQSTSSVLIQSLRCNSGASETSPAPQIQNEQGDENSSCHNRDIAMAYKNKATPMPNGAIVSASSMGNMHGEFLEKTSQYNPEHVFSGTQTTTSFTNAINTRAINVLTCGTSHLLHTSGQISSAQTSNSELPQVLASVATEIHNIENSTKGSIIQASTNFHHPEIMNSEQPKSGNAYQPPTNSREVSENAGQVPSQDFSFSSNNNLKTHDSSSEVLSQPREETGASFQQDSVFRNDSFNLPPIAPPSPGQEMNCVLSAAGSEDPRNIFNLTEENNRGQQHHQGEHAKKVSGFCQQTPSSQQDHQYKIQASQGNGSETNVDLSTVILGIKQQDPSETQSKLESKIQDPQRLRNSKELQHFPHLLSQINQQTTLDHDKDLVKNHLEQSQTHSQASWMRMTSPPFHPSDSSPKSNETILRTMLQLQTNSPEQTHPKQYIRGSNESKGAIGQSCYQNIMQGEQIPALFKSEGAQLPHHFTPDLHVPFQKHSPQSKKMNDQQQNSRHFNLQPQSELHLEQALKAPLKPQHLDLPFEDDQFIHSQILPPILQTQPIQPPQNSQVTMNPTQQIKTTKLSQIFPQSSNSQEQQGEGTSLNRLKLEECFEVGSNYYKSVEFPVQVPQTRVDHISNTANKISHYNQTNNAMKHSCSNNMHLVSEKKEDPINADLLTQNISRNLQHMQYYPNSITPNQDLPQGFQEQNPPSPPTSFLHLPFQQTQEYSHMTTNMNNSQASQIQPTYSPYSQQAASHAIDQSHGHCLPSQPHKDFQKHAAIRWHILNKQANQNKAESCQAMGFKSVKTETGSKVNACLPPPVGQLENKPWKKAIKQEVQHFGCENMQQRSIIETMEQQLKQFQVKTPFDHKSLTIKSPKQVKIEAAGPITILTRNCNGTDFNSHTTSLEQQAIHSNEKTPTKRSAGTALNNFLESPSSLLDTPLKNLLDTPVKTQYDFPSCSCVGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG95</accession>
    <name>A0A6I9YG95_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CAP-Gly domain-containing linker protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923293.1">
        <property type="nucleotide sequence ID" value="XM_014067818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG95"/>
    <dbReference type="GeneID" id="106550038"/>
    <dbReference type="KEGG" id="tsr:106550038"/>
    <dbReference type="OrthoDB" id="5412539at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="807"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="972"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="342"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="990"/>
            <end position="1000"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013923293.1"/>
        </source>
    </evidence>
    <sequence length="1000" mass="114704" checksum="91007A8494C466D2" modified="2020-10-07" version="1">MLAAARKEIELMSDEMRALIAEKEVLAQEGNALKLEKGSLLSKLVDLESKIGLLKQDQEKLWTVNEELNLENKRTVKEKQDAENRTQQEKEKNDELISEKGSLLLEIEAAQEDLLKITRENDALRTSKAALLSEVEELQARSEALAQECRKHVNQREELEDCQRKLSEENAALLKDKDDVIQKLKSSYEDMARDQKELLQEVTTLAAERDSLLGKFLDLENKHVSLKRERDSLLQTSQDLQSDKEALLKAEEERRQNLEQALEERRELQLKSEGLHSRLELSDKELRRVTKEKNQLEASHASLSKFLEEIKASRASTDTELIQLLQEKEALVCSERRLAAEREELWNENKNLSEKLAQTSEEAARVEKTLNDKLSQLSAEKEVASQKSLKFKKQNEGLVKEKASLEAKCAELLAEKQSATKAVCELKKKHELDISSKRMLVQEKAKLQGSAETLKRELDQKITENQDLVRYKAELSKILKETQSAKTVLENEYSALLHAKQLLSASFKSSSSEKEVLNREKVQLQEECQKLSKELKTIYNNLTIEREGRTLEKESFLVENTQLHNAVSRLEAEKEELSQKNQELLAERDQLKEEKLKSESRLEEIMKEKEVLGAETDQLASNIEKLKKDFAALTKSKAELQELHSSVSKILEDLRSSHQTSELERSKLLQENEVLLLEQKHLLAIKEEILKEKETFSKDYYKLHEEITLLAQANSEMSGNLLAAQNKTQALQKERDELVFKLEDIEGLQAQAVMQRFETAKTAEDALQIVEQVSKEKETLHREKTEISAALEEAKQLNQKLKKELDALKESHRKNREALTKSQERLNNESQNLDELRKELEILKLAEEEKSQQLAVFQEENLKLAKELGKGDLASHQKLEEERSVLNNQLLEMKKREATLIKENDEERASLQKSISVTSALITERDEELEKLRNEVSVLRGENATARNLHSVVQSLESDKVKLEIKVKNLEQKLKESQKSSSDVSSSGDFQKEDAQESQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGI0</accession>
    <name>A0A6I9YGI0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein kinase 40</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">STK40</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be a negative regulator of NF-kappa-B and p53-mediated gene transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013923015.1">
        <property type="nucleotide sequence ID" value="XM_014067540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGI0"/>
    <dbReference type="GeneID" id="106549798"/>
    <dbReference type="KEGG" id="tsr:106549798"/>
    <dbReference type="CTD" id="83931"/>
    <dbReference type="OrthoDB" id="410920at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13974">
        <property type="entry name" value="STKc_SHIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000269">
        <property type="entry name" value="Serine/threonine-protein kinase 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024236">
        <property type="entry name" value="Ser/Thr_kinase_40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF16">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="37"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013923015.1"/>
        </source>
    </evidence>
    <sequence length="437" mass="49837" checksum="556291C1B0B1F65C" modified="2020-10-07" version="1">MELRMKRRTSDRGGGETSIKVLCPGISSSNTKRAGPFILGPRLGNSPVPSIVQCLARKDGTDDFYQLKILTLEDKKDHGTETQEERQGKMLLHTEFSLLSLLHNQDGVVHHHGLFQDRTCELVEDLESSRMVRKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHKKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEDDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLAASEVLESLSSIITSWQSMSSLSGPLQVVPDVDDQVVNPEHAHEAKVSEECSQYEFENYMRQQLLLAEEKNTLHEAKHFLQKRQFGSIPPVRRLGHDAQPMNALDAAVLAQRYLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGL4</accession>
    <name>A0A6I9YGL4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho guanine nucleotide exchange factor 40-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550058</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923316.1">
        <property type="nucleotide sequence ID" value="XM_014067841.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550058"/>
    <dbReference type="KEGG" id="tsr:106550058"/>
    <dbReference type="OrthoDB" id="6152532at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052231">
        <property type="entry name" value="Rho_GEF_signaling-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45845:SF5">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45845">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="1116"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="1303"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="929"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1012"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1458"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1480"/>
            <end position="1489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923316.1"/>
        </source>
    </evidence>
    <sequence length="1545" mass="169579" checksum="B5285E16CBC6F8A1" modified="2020-10-07" version="1">MEPEPVEECVQSTLAALYPPFEATAPTLLSQVFEVLERTYQHDALRYTLDFLVPAKHILARIQSEACAQYSGFVFCHEGWPLCLHEKVLVQLGSLPWQHLSPGDFYLQVVPHRECAPRLVLKCLSLESRGVQELPVSPDSYRFLFTIEWLNGINKERRVGRLERCLLAAGEKVLSLPWAELVYPQFVHKDGFIVGQHCPTDLTPEILGARSPGDGRHSGGENRESEGEYVHLLEVSPTLHQAPRIPQPCTAQIQTMPARKGHSKNRNRRHRAWLHHKSGCGENFALRQACKTEEAEKSSQEKGNQDEKVAQLRSNPSPALDPVLESPGSVGNLNLKQLEENLDPLETKPSPILSSSDAVAEDTVLGKEEFLESVYRDPTPPKEESKESLSPALATPKRTTKGNRRKKKGAGKGAGGRVKQGSDSANQETESSDLKTGAEKEEAFTGPGEQDSGTVYVSTRDPDSIKVSCVDITECDDAPECDDTQEKGSTAGNQSFETGVEGANNADLAVSIGDCIASATEPEDSKGKCTIGDNEGSSGEGNTEDPKLNTEEPNAKPKTSEPRIDTEEVSVQEKEVEISEDQCMVEQRLDSKEPGLTDLPSKNELASVDGKEETVDIAADQGPGEIQNSLLPTVARLGESVNWNLLQSGVFALTGGIDKAGHSLLTIVPQPLQDELPPGQEDLVNTLRYMHSLLRQELKILGLTVLLDLSHVDDWAFQAPVLLPALRAFQETTPTPISQLLVILPSDDRFVPHEEELTLEPAVEIRLLLLHELPHYVALDQLTPALGGTLSYSPPKWVREHQALEQLQDLCCGVLQTVCEASDHLNAVGTASSQEELSTQIALHQDTMQKLLSEPRLLELQRRGGSLLARLPPSGPCSNQAVDATRLYQEVDEALHGLVQLSNRCLELLQQEKKNLQVQQEGKTCLLSSDSSVEKKRGGQRPAEGLAEGTSLEKKLLQYPRSSGKSQVTPKDSSVPRARACSISASSSPKHGLLLGAKFGSSCSLTSLFQPLTSPKGSPRCSPGRSNALDSRKKSQGPLSTSKTPASPANNLALRALSDPGRPSVHIRGLEVSSRELADRSCSPREHVLLGRSRTHVAEAPWGATPRMERRRCLGVQQQLLVELLECEREYVGALTQPFSLSQELGGHAWTAELRCLGSALRATRDRLLAFHSSCLLSELEGCMSHPSRAGSCFLRHREELQLYAIYAKDHHKFHAALALLRSTGKKGLMEPREDGQIAHTLQVPLEQLERYRRFLGELLHECELEQGPDRQTLQEAQQLLESQEQRGRDLLAIEQIRGCEMRLSEQGTLLQRGELILLSGRRKCQRHVFLFEQLLLFTKCKGAEGGYVCKQALQTASMGLTESVGSSGLRFELWFGRGSSRQAYTLQAASAEVKHQWTNIIAQLLWRQAAQPSVSMSLSSCPSQRLAPLGLGPGPPPGLSLPGHFEEEEWDLDVKPIPRAHTSGSEGTSPSHHEPLRQGRSSLPGSSSALGQHQSVSLFSVEMFFGDRICNLSSFINLILFLDCIISGYMWGKGNCARLAFYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGT7</accession>
    <name>A0A6I9YGT7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Apelin receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106549869</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923100.1">
        <property type="nucleotide sequence ID" value="XM_014067625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGT7"/>
    <dbReference type="GeneID" id="106549869"/>
    <dbReference type="KEGG" id="tsr:106549869"/>
    <dbReference type="OrthoDB" id="5974286at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF953">
        <property type="entry name" value="APELIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0306">Gastrulation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="183"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="10"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013923100.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="37552" checksum="FC07499D26ECC844" modified="2020-10-07" version="1">MLVFVLGLSGNGLVIFTVWRGSPGKRRSADAYIGNLALADLAFVVTLPLWAAYTALRFHWPFGSALCKLSSYLVLLNMFASAFCLACLGVERYLAIVHALPSRPSAAASSSSSPSSGCCASPRPRTWTLLSLAAVWLLAGLLALPALLLRQTRLSPRGGNGSALECDMDFSTLAGSAEQAARWLAALSLATTALGFVLPLLLLTLCYCCIGAAVSRHFRRRGRKEESRQRRRLLRILVTLVAVFALCWLPFHLLKSLYVLAWVGLLSLPCAWLDLILRLHPYATCLAYVNSCLNPFLYAFFDGRFRAQCRLLLGLRSPRRGPAGSVSSTLSGQTQKSELPSSGTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH13</accession>
    <name>A0A6I9YH13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106550011</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013923264.1">
        <property type="nucleotide sequence ID" value="XM_014067789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH13"/>
    <dbReference type="GeneID" id="106550011"/>
    <dbReference type="KEGG" id="tsr:106550011"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="54"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013923264.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="36737" checksum="7BB4E02AE73D8E30" modified="2020-10-07" version="1">MTQKQLSKFLDEKLEENEKATPSERTFMYDGVKYPTMICCLETLKRVNTFRARKDDIILVSYPKTGSNWVGEILRQLEIASGKYDEVEQKQRQQILEELSLLPFLEFGDPEKFERMEKLPPRRIIKTHLIPHMLPRSFFEKKTKMLVLFRNPKDVAVSFFHFAKGIHMNDQNTWDEFFEEYITGKVTYGSYFDHISEWNKHLNDSNVFFITYEEIKENPVSALKKIAKFFDFSVTEEEIESIVEKTSFESMKNTAENYGKLGKALFRKGIVGDWTSVFSESQNEKMDRKFEETVAKTQLGMKLKYELYCKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH14</accession>
    <name>A0A6I9YH14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Amino-terminal enhancer of split</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AES</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat Groucho/TLE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923608.1">
        <property type="nucleotide sequence ID" value="XM_014068133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH14"/>
    <dbReference type="GeneID" id="106550262"/>
    <dbReference type="KEGG" id="tsr:106550262"/>
    <dbReference type="CTD" id="166"/>
    <dbReference type="OrthoDB" id="8949191at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005617">
        <property type="entry name" value="Groucho/TLE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009146">
        <property type="entry name" value="Groucho_enhance"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814:SF31">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03920">
        <property type="entry name" value="TLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Groucho/TLE N-terminal Q-rich" evidence="10">
        <location>
            <begin position="18"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923608.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="21964" checksum="7A5A039A4BFDB528" modified="2020-10-07" version="1">MMFPQSRHSASSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQQLQAHQLSQLQALALPLTPLSVGLQAPSLPAVSASTGLLSLSALGSQAHLCKEDKNGHDGDAHQEDDGEKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHI1</accession>
    <name>A0A6I9YHI1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Low-density lipoprotein receptor-related protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LRP10</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <location evidence="10">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923783.1">
        <property type="nucleotide sequence ID" value="XM_014068308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHI1"/>
    <dbReference type="GeneID" id="106550405"/>
    <dbReference type="KEGG" id="tsr:106550405"/>
    <dbReference type="CTD" id="26020"/>
    <dbReference type="OrthoDB" id="9990982at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000050">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF17">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026799489" evidence="15">
        <location>
            <begin position="30"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="16">
        <location>
            <begin position="40"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="16">
        <location>
            <begin position="153"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="488"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="604"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="562"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="604"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013923783.1"/>
        </source>
    </evidence>
    <sequence length="653" mass="71140" checksum="2B661D23FE88075C" modified="2020-10-07" version="1">MGARQPLPAKRRGAPCLILLGLLLGVIQAVESNSSRTSVCPGYPDVRKEPQGQIRSPSGRVMTSSSCSWVIVGGPDDIIYIGFQHFFLSCGSEMLIIRAPNQPSRKLCGYTTPEPIRVKGGNVTLTYSWKFWARNRFQLKYEREPKTQPVVSCNRTLDDFYGVIYSPAFISDSYLGAAHCQWLLDTHDSRPLILRFTVLNLDIGDSLRVYDGAPGAPLRPHLLRNLDYTSNNKIVSVDSPSSRAWVVYQAVPGTEGRGFNATYHVQGFCVPWDFPCGGDSGEGATCYSTTERCDGNWDCANGADEDGCHGCPPGQYACGGRGIGGAVCYGPADRCNYQTFCADAADERRCYSCQPGNFRCRDGRCIYESWVCDGQADCVDTSDEADCSYILPRKVVTAAVIGSLVCGLLLVIALGCTCRLYALRSHEFGVLAPMSRMEAQLVQRQAPPSYGQLIARGVIPPVEGFPVEHPNQNSVLGNLRSLLHILRRDPASPPGAGRGRRPHQSRFVRRLTRRMRRMGLLRHPTSPASSSTGASNSSSAPADLAEPSRGDGDASFQLPENQPAPLPVKVPPTPAEPTPDLHIPRPTPRFAGVMSSLRVRLFSSTPTCSAESGDNRNTSPSPNDEDEVLLVPLTDPQLDNFANWNADDEPLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIK8</accession>
    <name>A0A6I9YIK8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">A disintegrin and metalloproteinase with thrombospondin motifs 16-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924052.1">
        <property type="nucleotide sequence ID" value="XM_014068577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIK8"/>
    <dbReference type="GeneID" id="106550640"/>
    <dbReference type="KEGG" id="tsr:106550640"/>
    <dbReference type="OrthoDB" id="412680at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF140">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="ADAMTS/ADAMTS-like Spacer 1" evidence="3">
        <location>
            <begin position="21"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013924052.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="16924" checksum="B87E07F7C45030CF" modified="2020-10-07" version="1">MEHKTQPVTMKKHYCPCCDIKYYTLVTIPAGARSINVYEMNVSNSYIAVRTSSKKYHLNGYWTVDWPGKYKFAGTLFVYQRPYNHPEILSASGPTNESITIQLLSQGKNLGIAWEYSLPKVETKRTHVLKHNYTWAIIRPKCSVSCGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJF7</accession>
    <name>A0A6I9YJF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106551019</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Golgi apparatus membrane</location>
            <topology evidence="2 18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_013924498.1">
        <property type="nucleotide sequence ID" value="XM_014069023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJF7"/>
    <dbReference type="GeneID" id="106551019"/>
    <dbReference type="KEGG" id="tsr:106551019"/>
    <dbReference type="OrthoDB" id="9924649at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000017">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.460">
        <property type="entry name" value="ppGaNTase-T1 linker domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF50">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 18" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="19">
        <location>
            <begin position="297"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013924498.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="50060" checksum="0A3965276CC6FB04" modified="2020-10-07" version="1">MRVLKKHPVFSREDEWDSTRVSHRFRVLTSTYLDVTEVGKSIGSLQDLWPSYTIARFEYTSGHEALKLIPLKSAAFYLFFLSEELKAPLEEHIKKFNGKHPGLLKIVRHTERKGLTEARISGWRASRADVVAILDAHIEVNTKWAEPILSRIKEDHTVIISPVFDNIRFDDFQLTQYAEAADGFDWALWCLYESIPEEWYALKDQTAPIRHTGHVTLEVSLDNAGSLTDDRCPQEPAELRICHLFETDVLLFQNSGIDYGDVSSRKELRKQLNCKSFDWYIKNVYPNLVLQVNIVGYGTMKNKLSEENCIDQGPVPGNTPILYVCHGYSPQHMFYHSTGEFFVGGLRSRRNTVDRCLTDPGEGNLPVIEQCDDAQRKGLNMHWDFMQGSSVTNRKTKRCLELAKLNGSPYYTLVMQVCTGQKWHIQNTVNQWGKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLH3</accession>
    <name>A0A6I9YLH3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TBC1 domain family member 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106551126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924620.1">
        <property type="nucleotide sequence ID" value="XM_014069145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLH3"/>
    <dbReference type="GeneID" id="106551126"/>
    <dbReference type="KEGG" id="tsr:106551126"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000005">
        <property type="entry name" value="TBC1 domain family member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF15">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 12 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="269"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924620.1"/>
        </source>
    </evidence>
    <sequence length="365" mass="42711" checksum="977FDAAB0A8D066D" modified="2020-10-07" version="1">MFASARALASPLLVYQLNWETLMTKLCNHYAPMLSRIARRYVFRQCIQKESETVSQFLQSLRMVMIQCEFIDLDNSLVEQFICGIKDPRLRRRLLAWAEITLQQAVDEGRAAELSEQSTADMQQLQMPPPPVRACLIDELVRLDELEDNHEYEARIGKFCKPAPQATRRRHFEFEPLSTTALILEDRPSNLPAKSTEEALRHRQEYDEMVAEAKKREIKEAHRRKKIMKERFKQEENIASAMVIWVNEILPNWESMRSTRRVRELWWQGLPPSVRGRVWSLAVGNELNITPELYEIFLSRAKERWKIFGEIGTENDTEDTGASLADREASLELIKLDISRTFPSLYIFQKVTFIDYAFEITLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YM45</accession>
    <name>A0A6I9YM45_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein Dok-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DOK7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925302.1">
        <property type="nucleotide sequence ID" value="XM_014069827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YM45"/>
    <dbReference type="GeneID" id="106551684"/>
    <dbReference type="KEGG" id="tsr:106551684"/>
    <dbReference type="CTD" id="285489"/>
    <dbReference type="OrthoDB" id="6537982at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037746">
        <property type="entry name" value="Dok-7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21636">
        <property type="entry name" value="PROTEIN DOK-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21636:SF2">
        <property type="entry name" value="PROTEIN DOK-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013925302.1"/>
        </source>
    </evidence>
    <sequence length="455" mass="47913" checksum="E0217433BBF835D8" modified="2020-10-07" version="1">SFTGAGVFFLSCTEGEKISFLFDCIVHGICPTKGFRRALADPSVNPACTEERVAYEASKLEKRLSLLSHSGCHGSGGDDRSLSSASSDTSHSDASIGSRLTLWPEQSSAGTSQESRGPVAAAVAAAAAAERLQSDGAQGASKPPPSLLRSRQLQEIGRQSSSDSGIATGSHSSYSGSFSSYTGSLDISHGDEFGSLLSLSLSFPSDRNVCSCRSPGSEYQAPSWLRHNYDTPRSLPAASTTGIFAAGPDAGLSKAQSSKGDGKRPAPHLEREAANRPSQDKDSQGVSPSGSIKGSIPETQFRPPVQGQDWRGPEIAPCVSGGSCELCSSPSEMKTALFPSCPVCGGIQETSILPSQMFAPNLEPSLSEQLHGEMATYVNIPTSPTAKKQLHYMEVELPEPSGSVRGGDATKYAQIDITATETAHKVGTQHAYFREERLQELEQQQKKDGVPPGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPI5</accession>
    <name>A0A6I9YPI5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hippocampus abundant transcript 1 protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Major facilitator superfamily domain-containing 14A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HIAT1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926066.1">
        <property type="nucleotide sequence ID" value="XM_014070591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPI5"/>
    <dbReference type="GeneID" id="106552342"/>
    <dbReference type="KEGG" id="tsr:106552342"/>
    <dbReference type="CTD" id="64645"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17387">
        <property type="entry name" value="MFS_MFSD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000099">
        <property type="entry name" value="Hippocampus abundant gene transcript 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF77">
        <property type="entry name" value="HIPPOCAMPUS ABUNDANT TRANSCRIPT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="430"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="13">
        <location>
            <begin position="37"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="457"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926066.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="52829" checksum="DF315DF0761F107B" modified="2020-10-07" version="1">MTQVGKKKKRAANRSILLAKKIIIKDGGTPQGIGSPSVYHAVIVIFLEFFAWGLLTAPTLVVLHETFPKHTFLMNGLIQGVKGLLSFLSAPLIGALSDVWGRKSFLLLTVFFTCAPIPLMKISPWWYFAVISVSGVFAVTFSVVFAYVADITQEHERSMAYGLVSATFAASLVTSPAIGAYLGRVYGDSLVVVLATAIALLDICFILVAVPESLPEKMRPASWGAPISWEQADPFASLKKVGQDSIVLLICITVFLSYLPEAGQYSSFFLYLRQIMRFSSESVAAFIAVLGILSIVAQTIVLSLLMRSIGNKNTILLGLGFQILQLAWYGFGSEPWMMWAAGAVAAMSSITFPAVSALVSRTADADQQGVVQGMITGIRGLCNGLGPALYGFIFYIFHVELNENPMSENEVNVPPQHHSQQNSIIPGPPFLFGACSVLLALLVALFIPEHTNLNGRSSSWKKHCGGHGHPHSPQAPGEAKEPLLQDTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ35</accession>
    <name>A0A6I9YQ35_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BTB/POZ domain-containing protein KCTD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KCTD2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926024.1">
        <property type="nucleotide sequence ID" value="XM_014070549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ35"/>
    <dbReference type="GeneID" id="106552306"/>
    <dbReference type="KEGG" id="tsr:106552306"/>
    <dbReference type="CTD" id="23510"/>
    <dbReference type="OrthoDB" id="1244179at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18389">
        <property type="entry name" value="BTB_POZ_KCTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2000:FF:000001">
        <property type="entry name" value="Potassium channel tetramerization domain-containing 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000005">
        <property type="entry name" value="Potassium channel tetramerization domain-containing 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14958:SF22">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14958">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERISATION DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="43"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926024.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="25875" checksum="58DA713B7704DB5B" modified="2020-10-07" version="1">TPSPLPISGPPSPRPAVAPTCPARSPEPPPPEKAAAGGGGGSKWVRLNVGGTYFVSTRQTLCREPKSFLCRLCCQEGPELGSDKDETGAYLIDRDPTYFGPILNYLRHGKLIINKELAEEGVLEEAEFYNIASLVRLVKERIRDNESRTSQGPVKHVYRVLQCQEEELTQMVSTMSDGWKFEQLISIGSSYNYGNEDQAEFLCVVSRELNNSTNGIVIEPSEKAKILQERGSRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQM1</accession>
    <name>A0A6I9YQM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">TXNDC9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Significantly diminishes the chaperonin TCP1 complex ATPase activity, thus negatively impacts protein folding, including that of actin or tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms ternary complexes with the chaperonin TCP1 complex, spanning the cylindrical chaperonin cavity and contacting at least 2 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926461.1">
        <property type="nucleotide sequence ID" value="XM_014070986.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926462.1">
        <property type="nucleotide sequence ID" value="XM_014070987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQM1"/>
    <dbReference type="GeneID" id="106552656"/>
    <dbReference type="KEGG" id="tsr:106552656"/>
    <dbReference type="CTD" id="10190"/>
    <dbReference type="OrthoDB" id="10257948at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd02989">
        <property type="entry name" value="Phd_like_TxnDC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000141">
        <property type="entry name" value="Thioredoxin domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21148">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="77"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="20"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013926461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926462.1"/>
        </source>
    </evidence>
    <sequence length="224" mass="25945" checksum="B82B4319CC568F50" modified="2020-10-07" version="1">MNADTSVFSQAVEASMLQTTKIVEEELDAEIQKLDQMDEDELELLKRRRLEALKKAQQQKQEWLSKGHGEYLEIPSERDFFQEVKGSKNVVCHFYRDSTLRCVILDKHLTILAKKHIETRFIKLNAEKSPFLCERLRIKIIPTLALLKDGKTQDYVVGFTDLGNTDEFTTETLEWRLGCADIINYSGNLMEPPFQSQKKPGTFFTKLDKKTIRGKKYDSDSDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSR2</accession>
    <name>A0A6I9YSR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 2, facilitated glucose transporter member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Fructose transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glucose transporter type 5, small intestine</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SLC2A11</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose(out) = D-fructose(in)</text>
            <dbReference type="Rhea" id="RHEA:60372"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926974.1">
        <property type="nucleotide sequence ID" value="XM_014071499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSR2"/>
    <dbReference type="GeneID" id="106553071"/>
    <dbReference type="KEGG" id="tsr:106553071"/>
    <dbReference type="CTD" id="66035"/>
    <dbReference type="OrthoDB" id="8120565at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005353">
        <property type="term" value="F:fructose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990539">
        <property type="term" value="P:fructose import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001511">
        <property type="entry name" value="Solute carrier family 2, facilitated glucose transporter member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF22">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="9 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="15">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013926974.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="41618" checksum="5D57136F264B147D" modified="2020-10-07" version="1">MILLSRFFAGINSGVAMSIQPMYLGESAPKELRGAVAMTSASFTALGLVLGQVVGLREILGAEESWPILLASNVVPGLIQLVLLPWAPESPRYLLIDQGDQDSCISALQQLRGSSDLSSEMKELLAEQAALQGETTKAPWELWRNRAVRWQFITVVVLSSAMQLCGNDSMYFYASYVFQEAGIPYDKIQYSVIGTGSCELIMSMTCNLLIECVGRRVLLIVGYSLMATWAILFMVALSLQDHLSWMPYLSMACIFAYILSYGIGPAGVTGIIPTEIFDQVSRPAAYTISGSLLWINLFLVGLAFPFVMESLGYYCYLPFFTVCVGTTLYVWFFLPETKGKSFLEISEEFVKRNFECQPYDDAWVYPEEIISTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSZ1</accession>
    <name>A0A6I9YSZ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aquaporin-5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106553158</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927074.1">
        <property type="nucleotide sequence ID" value="XM_014071599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSZ1"/>
    <dbReference type="GeneID" id="106553158"/>
    <dbReference type="KEGG" id="tsr:106553158"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015670">
        <property type="term" value="P:carbon dioxide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF38">
        <property type="entry name" value="AQUAPORIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927074.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="35267" checksum="4777D9A59BE11264" modified="2020-10-07" version="1">MPPLPTIFRAFSLAREIVWDILTSKYSSPDSKIILRVTDRFLFGPTYTWLNPSLSLYKCMQHGPANLLPARGAYKFHVNTHSVSTAPAMATTERQAEILSPKFAWAILSEFVGTAIFLCIALGASFKWPMEQPNVLQISLAFGLTVATMVHIFGPISGAHINPAVTLAYFVGNQISIVRFVFYMILQVLGALAGTGIIYAVTPGEIRSTLFANHVPKEVTAGEAFVVELILTFSVVMCIFSVNDKRRRESRSYAALSIGLAVAMAHLIGIYYTGCSTNPARSFAPAVILGTFGPFHWFFIFRQAFRQIMLIRNGPYELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT67</accession>
    <name>A0A6I9YT67_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ubiquitin-conjugating enzyme E2 J1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">E2 ubiquitin-conjugating enzyme J1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Non-canonical ubiquitin-conjugating enzyme 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">UBE2J1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) and is essential for cells to recover from ER stress. Plays a role in MAPKAPK2-dependent translational control of TNF-alpha synthesis. Also acts as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the E3 ubiquitin-protein ligase RNF26. Plays a role in male fecundity through the interaction with the E3 ubiquitin-protein ligase RNF133.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the HRD1 complex, which comprises at least SYNV1/HRD1, DERL1/2, FAM8A1, HERPUD1/HERP, OS9, SEL1L and UBE2J1. Interacts with E3 ligase RNF26. Interacts with E3 ligase RNF133.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927512.1">
        <property type="nucleotide sequence ID" value="XM_014072037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT67"/>
    <dbReference type="GeneID" id="106553517"/>
    <dbReference type="KEGG" id="tsr:106553517"/>
    <dbReference type="CTD" id="51465"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000043">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="19">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="209"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="209"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013927512.1"/>
        </source>
    </evidence>
    <sequence length="304" mass="33869" checksum="036C440D2300D512" modified="2020-10-07" version="1">MKEAAELKEATDHYHAQPLEDNLFEWHFTVRGPPDSEFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFYCEGCGSLMKEVLLPLTSGSVSSQADQEAKELARQINFKAEGNSSRKSVTEPLDMSEFNHLGNSSEHQQEITTPAFQDTTSSEMSGNPTSSATSNQEEIHSQPASNNTSVSPRQRHNQHRRAPGLTNFNQVQQPRVNTSHMRSNMLILLLVLALAVLVVRRIYLANEYVFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU77</accession>
    <name>A0A6I9YU77_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquitin carboxyl-terminal hydrolase 48-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928133.1">
        <property type="nucleotide sequence ID" value="XM_014072658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU77"/>
    <dbReference type="GeneID" id="106554059"/>
    <dbReference type="KEGG" id="tsr:106554059"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01795">
        <property type="entry name" value="Ubl_USP48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044743">
        <property type="entry name" value="Ubl_USP48"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="441"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928133.1"/>
        </source>
    </evidence>
    <sequence length="527" mass="59630" checksum="F713F2A44261352E" modified="2020-10-07" version="1">MVPCLPAACLPPCLDDWMNDWIDGWVASCLDAFHPPSSSSVKALCKDCVVERCRVLRLKSRLSEDYKAVCNLLKAAVKGSDGFWVGKSSLRSWRQLALEQLDDSDEDTEPSNGKMNGDAKEREEPSAEKGGEEEELKFNEDIVCPHGNLCILENERRVVCRDAWEKLQRYFRNAPEFPSSRDSCSQCKILEREGEENEALHKMMASEQKASLPHLFQDKTRPCLSSWPEEVGELYVVSRFFVEEWRKFVRRPTRCSPVSSVGNRELLCPHGGLMFTVASMTKDDSKLIALIWPDEWEKIGKLFVVDHAIRVIRREAPQESAERLLYMTEPELCLDCREGLLYQQQRDLREYTQATIYVRKVVDDKKVMKEAAPELNVSSSEAEEEREEARAEGEHDPDFNQANSNAKRQKVSHPNSVVCQKQGIRRSTRHRRIRGEKALLVSANQTLKDLKIQIMHAFSVAPFDQNLSIDGTVLSDDVATLGSLGVTPESVVLLKADEPIVDYAAIDDVMQVCIPEEGFKGTGLLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU80</accession>
    <name>A0A6I9YU80_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Claw keratin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106553542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avian keratin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927544.1">
        <property type="nucleotide sequence ID" value="XM_014072069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU80"/>
    <dbReference type="GeneID" id="106553542"/>
    <dbReference type="KEGG" id="tsr:106553542"/>
    <dbReference type="OrthoDB" id="9049954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003461">
        <property type="entry name" value="Keratin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203:SF1">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02422">
        <property type="entry name" value="Keratin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0416">Keratin</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927544.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="15723" checksum="2A1537909FFE4769" modified="2020-10-07" version="1">MVDCGPSCAVPSCASAPVVGFGSAGSKGLGCGPLGYGNLSYGGLGYAGLGYGSLGYGGLGSGLGYGLGAGALAESSGSLGTLAGVIPNCINQVPPSEVTIKPPAVVVTIPGPILSASCEPVAVGGNTPCAPGGIGGTGGFGGYGRVGGVYGGRLGRLGRRGSLCNLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUN4</accession>
    <name>A0A6I9YUN4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SRP54</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). As part of the SRP complex, associates with the SRP receptor (SR) component SRPRA to target secretory proteins to the endoplasmic reticulum membrane. Binds to the signal sequence of presecretory proteins when they emerge from the ribosomes. Displays basal GTPase activity, and stimulates reciprocal GTPase activation of the SR subunit SRPRA. Forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SR subunit SRPRA. SR compaction and GTPase mediated rearrangement of SR drive SRP-mediated cotranslational protein translocation into the ER. Requires the presence of SRP9/SRP14 and/or SRP19 to stably interact with RNA. Plays a role in proliferation and differentiation of granulocytic cells, neutrophils migration capacity and exocrine pancreas development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The M domain binds the 7SL RNA in presence of SRP19 and binds the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="16">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit SRPRA. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928293.1">
        <property type="nucleotide sequence ID" value="XM_014072818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUN4"/>
    <dbReference type="GeneID" id="106554202"/>
    <dbReference type="KEGG" id="tsr:106554202"/>
    <dbReference type="CTD" id="6729"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.30:FF:000002">
        <property type="entry name" value="Signal recognition particle 54 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.140:FF:000003">
        <property type="entry name" value="Signal recognition particle 54 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11 16" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="17">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013928293.1"/>
        </source>
    </evidence>
    <sequence length="526" mass="58709" checksum="CEDF2DA50075B997" modified="2020-10-07" version="1">MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNIIMFVGLQGSGKTTSCSKLAYYYQKKGWKTCLICADTYRAGAFDQLKQNATKARIPFYGSYTEMDPVIIAAEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVNPSQMAKLNQQMAKMMDPRVLHHMGNYAFSFLAAEVGWKRTTSYQFNKIDYLPNILETELSHFAMTKLYLWYSYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWT1</accession>
    <name>A0A6I9YWT1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myc target protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MYCT1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928060.1">
        <property type="nucleotide sequence ID" value="XM_014072585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWT1"/>
    <dbReference type="GeneID" id="106553988"/>
    <dbReference type="KEGG" id="tsr:106553988"/>
    <dbReference type="CTD" id="80177"/>
    <dbReference type="OrthoDB" id="9943706at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029180">
        <property type="entry name" value="Myc_target_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14869">
        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14869:SF0">
        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15179">
        <property type="entry name" value="Myc_target_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928060.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="21362" checksum="34D348AE3C4712C0" modified="2020-10-07" version="1">MDNSTSQENFWPPNFWNQIIIAFSVSMVIGLVIGSIFWGVLTCLSSRRASAHISQWSTSSSNWRTRAAHGCVASRPGFYRNSICERRSNLSLASLTFQRQASLEHGNSFPRKSSFRASTFHPFLQCPPLPVETESQLITLVPTSTTTTPTGTSTNSFTHPEFTWSNNSLRVFHSTPTPPPAYDSIIKAFPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXV2</accession>
    <name>A0A6I9YXV2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Gamma-adducin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ADD3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldolase class II family. Adducin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929493.1">
        <property type="nucleotide sequence ID" value="XM_014074018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXV2"/>
    <dbReference type="GeneID" id="106555231"/>
    <dbReference type="KEGG" id="tsr:106555231"/>
    <dbReference type="CTD" id="120"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000004">
        <property type="entry name" value="gamma-adducin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF5">
        <property type="entry name" value="GAMMA-ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="12">
        <location>
            <begin position="139"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="568"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="662"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="669"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="680"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013929493.1"/>
        </source>
    </evidence>
    <sequence length="704" mass="78853" checksum="75F1CD9BD73A9401" modified="2020-10-07" version="1">MSTEISQGVITTPPPSSMPHKERYFDRINENDPDYIRERNMSPDLRQDFNMMEQRKRVTQILQSPAFREDLESLIQEQMKKGNNPTGLLALQQIADYIMASSFAGFSSSPLSSGLITPINDLPGIDTTTFGKGEKLIRCKLASLYRLTDLFGWAHLANSYITVRVSKEQDHILIIPDGLSFSEASASNLVKVNIIGDVVDQGSTNLRIDTAGFSPHAAIYSMRPDVRCVIHIHTPATAAVSSMKCGILPISQEALILGDVAYYDYHGSLDDQEEKIDLQKVLGPSCKVLILRNHGVVALGETLEEAFHYIYNVQLACEIQVHALAAGGVDNLLILDLQKYKPFTHQVAAAAGLSMGSQFKWKVGELEFEALMRMLDNLGYRTGYAYRQPLVREKPRHKSDVEIPATVTAFSFDDDSIPISPLKFLAQKQQREKTRWLNSPNTYLKVDVPEESRNGETSPRTKTTWIKAEDSSKISGGTPIKIEDPNQFVPLNTNPSDVLQKRNKIREQNRYDLKTAGPQSQLLAGIVVDKKPTPMQFEEDEHTPPAPPNPFSHLTEKELEEYKQTIERKQQGTEDADQELFSDDGSPVSQINSQTQSPQNVPDKLEENNDNYLQDSNLISLELPVVVMNGKEDVHDVEEDLAQRVSQLTTSTVENIEITIKTSEKIEETLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXW7</accession>
    <name>A0A6I9YXW7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger protein 436-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106554600</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928786.1">
        <property type="nucleotide sequence ID" value="XM_014073311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXW7"/>
    <dbReference type="GeneID" id="106554600"/>
    <dbReference type="KEGG" id="tsr:106554600"/>
    <dbReference type="OrthoDB" id="9037488at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001997">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000739">
        <property type="entry name" value="Zgc:171418 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF379">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="19"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013928786.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="45945" checksum="6374969B811178FC" modified="2020-10-07" version="1">MMYVETKQENDDACTDLLGSEWKSETEGKPCGDFSEETEREPFEEKVWSRAQPTHRERDPQGEWIDKSLPCPVGELHPISLQENNEGGEERNGALRDCWEMSSVTETQKSMFGKNPGDRETLSEGESSYTFLENGEKFLGPLIPPSDRAVLSDGQHQPSDFNKTFHGPSTLLKPPGVPKVEKLYKCLECGKSFSRSAHLTSHQIIHTGEKPYTCLECGKSFVQSAHLASHQIIHTGEKPYRCSECGKSFNKSTNFLRHQKIHKGEKPHQCADCNKTFSDKPSLIQHQRVHTGERPYKCYECGKSFSHRGSLNAHQRMHTGEKPYGCSDCGKNFRDQSSLIRHKRTHTGEKPYTCSECGKSFSQSTNLTLHQKVHTEGTFPSDSPQILNTGMGVLVFPQNPANGLVALEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXZ3</accession>
    <name>A0A6I9YXZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AarF domain-containing protein kinase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ADCK1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Appears to be essential for maintaining mitochondrial cristae formation and mitochondrial function by acting via YME1L1 in a kinase-independent manner to regulate essential mitochondrial structural proteins OPA1 and IMMT. The action of this enzyme is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928816.1">
        <property type="nucleotide sequence ID" value="XM_014073341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXZ3"/>
    <dbReference type="GeneID" id="106554626"/>
    <dbReference type="KEGG" id="tsr:106554626"/>
    <dbReference type="CTD" id="57143"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF19">
        <property type="entry name" value="AARF DOMAIN-CONTAINING PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="4">
        <location>
            <begin position="82"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="4">
        <location>
            <begin position="221"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013928816.1"/>
        </source>
    </evidence>
    <sequence length="469" mass="53694" checksum="A02234A8D5537C34" modified="2020-10-07" version="1">MTHLKDIMLTAVITFDYLTSLRNFPRGTEEYEYVKSQVHLRSAERLRDLCCANRGTFIKVGQHLGALDYLLPEEYTRTLKILHSQAPQSSRQEIEQVIQEDLGKGINELFASFEDVPLGAASLAQVHKAVLQDGRTVAVKVQHPKVQAQSSKDILLMEILVLVVKQIFPDFEFMWLVDEAKKNLPVELDFLNEGKNAEKVAYMLRCFDFLKVWSMCDCMDAHIYSKTLIQWITISRNLGKIYSEMIFVNGFVHCDPHPGNVLVRKCPATGKSHIILLDHGLYQVLTDSFRLDYCRLWQALIKADIKQIKKYSQDLGAGDLYPLFACMLTARSWESVNKGIDRLPVTSNEDVEIRTNAATYLPQITKLLSNVPRQMLLLLKTNDLLRGIESALQTRANASSFLNMSRCCIRAVATYQRSKSNSLFRKTQISLSEALSLWQINMYELFLWLKISQLGNWVIAFLKRMHCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY99</accession>
    <name>A0A6I9YY99_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ERBB receptor feedback inhibitor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ERRFI1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928906.1">
        <property type="nucleotide sequence ID" value="XM_014073431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY99"/>
    <dbReference type="GeneID" id="106554705"/>
    <dbReference type="KEGG" id="tsr:106554705"/>
    <dbReference type="CTD" id="54206"/>
    <dbReference type="OrthoDB" id="9931672at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042059">
        <property type="term" value="P:negative regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045616">
        <property type="term" value="P:regulation of keratinocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052112">
        <property type="entry name" value="EGFR_SigReg_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14254:SF5">
        <property type="entry name" value="ERBB RECEPTOR FEEDBACK INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14254">
        <property type="entry name" value="GENE 33 POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928906.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="47723" checksum="2748BD2E982559BF" modified="2020-10-07" version="1">MSTTGIAAQEVNLSLNPGYLRNGSLEACCRRHNRQFANPFFHVDSVAMACNLNPGPNPLTSVGQVSCPDSDSGPSFLLRASPSLLHSPAPSASEEDRFVSGFGGLSLTMNRNSDETPSQIPGKSSPLLSLPLSSLERSARPLPPLPIPENFLHDEVDREVEFLTSSDTDFLLEKCASPSFKLSAQGRRSFRGCGQINYAYFDAPAEAKPQQPSCPENTCPPPPPPQQLHRRLRRSHSGPAGSFNKRSVRVPSHLCPSSPSSDDDKPEVPPRVPLPPRPLKPDYRRWSAEVTSGAYSDEDKPPKVPPREPLPWSHSRTPSPKMLPAYLHGVMPPTQSFAPDPKYVSSKALQRQHSDGSAHKVPCILPIIENGKKVSSTHYYLLPERPPYLDKYEMFFQEAEEGGLRPEEGGHEAATLVKADLGGLVKRKHLACMVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYD5</accession>
    <name>A0A6I9YYD5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mas-related G-protein coupled receptor member H-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106554729</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family. Mas subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928936.1">
        <property type="nucleotide sequence ID" value="XM_014073461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYD5"/>
    <dbReference type="GeneID" id="106554729"/>
    <dbReference type="KEGG" id="tsr:106554729"/>
    <dbReference type="OrthoDB" id="9043355at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000193">
        <property type="entry name" value="Mas-related G-protein coupled receptor member E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026234">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334:SF69">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02108">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="55"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928936.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="36626" checksum="0470B919C3EFC3A7" modified="2020-10-07" version="1">DDDYSGSGAFYNITPISFNERDVWAKYFGMTPVLDLTSNLINGFFAVICILGLVGNGRTIHLLAFSIKRNPFTTFILNLSLADFGVLTSFIVAAVVVAVLTLHGITHLSFAFFFLLLELFFFTYSASRLLLTAISLDRCVAVLFPHWHRSHRPPYFPTLLCLLTWNLSFFLSAVHFILYQTTNFGISLFFFQIIVNGLLCTPLMVVSTLTLWICMRSKPRHNQRKLLTTILLALLFFLLFSLPMDVFYVIEYFGSYNPLLMTVGIGCVALNSSINPLLYFLVGRKKRGKDQPRASYKVALQRVFKDEKDSPEDPKTMEEDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYR3</accession>
    <name>A0A6I9YYR3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">E3 ubiquitin-protein ligase UBR4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106555500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929843.1">
        <property type="nucleotide sequence ID" value="XM_014074368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYR3"/>
    <dbReference type="GeneID" id="106555500"/>
    <dbReference type="KEGG" id="tsr:106555500"/>
    <dbReference type="OrthoDB" id="30336at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045841">
        <property type="entry name" value="E3_UBR4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045189">
        <property type="entry name" value="UBR4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21725">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21725:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19423">
        <property type="entry name" value="E3_UBR4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase UBR4 N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013929843.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="58577" checksum="15D7A3020F46F664" modified="2020-10-07" version="1">TLASIIKEVDKDGLKGTSEEEEFAAALYHFNHSLVTSDLQSPTLQNILLQQLGVSPFSEGPWPLYIHPQSLSVLSRFLLIWQHKASTQTDPDVPECLNVWERFVATLKQNALQGILPGDTEDLNVEHLQLLLLIFHSFSEKGRRSILTLCVQTILDVTANLDSQLRCVPLLLARLLLVFDYLLHQYSKTPVYLFEQVQYNLLTPPIVWASASQEGSRPACSPLYHGFKEVEENWAKHCPSDAAPQPRFYCILSPEASEDDLNRLDSTVCEVLFSKAMKYDELYSALASLLAAGSQFDTLRRKENKNVTALEACALQYYFLILWRVLGLLPPSKSYMNQLAMNSPEMRECDILHTLRWSSRLHIPSYVNWIKDHLIKQGMKTEHAASLVELTSAKCSSVKYDVEIAEEYFARQISSFCGVDCTTILQLHEIPSLQSIYTLDAAISKVQVSLDEHFSKLAAETDPHKSSEITKNLLPAALQLIDTYATFTRSYLLQSLSEDSSAENKPTEEKLQGYAAVLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZ49</accession>
    <name>A0A6I9YZ49_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pleckstrin homology domain-containing family M member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106555080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929324.1">
        <property type="nucleotide sequence ID" value="XM_014073849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZ49"/>
    <dbReference type="GeneID" id="106555080"/>
    <dbReference type="KEGG" id="tsr:106555080"/>
    <dbReference type="OrthoDB" id="62364at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051366">
        <property type="entry name" value="DEF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025258">
        <property type="entry name" value="RH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326:SF5">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY M MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13901">
        <property type="entry name" value="RH_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01175">
        <property type="entry name" value="DUF4206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="48"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929324.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="57915" checksum="49D73EFC8D1D6C4C" modified="2020-10-07" version="1">MPLRVPDPGELSVSSAELSKSKLSPSRELKGFSVIHRRQIGLSNPFRGLLKLGTLERRGAMGIWKEVFCELSPLELRLFVDCEKRACVENCSLLRCESLGRAHSDGRFELVFSNKRLYLRASSRDEAEDWLDRLHEALKKYRLQQDEGWETLECPEAAEDSEDFFGGGPPGDLPSFSVYSRTGPTELDWSCALKPELDAVKESVLYLEVEKTWVPLVFSLSLEALKCFKARNDKKILYNSFGIETIQDILPDVSLGGPAFFKIITSKAVLKLRAENAEEATDWRDLVRRVLMSYLEMAEEALTLGGNLDGNSQAILKNTVKENGYLLQYLVAIPMEKGLDCQSFICAGCSRQIGFFFMKPKLCSFTGLYYCDNCHQDEESVIPSRLIHNWDLSRYPVCPVMPKRVQNQPLIDLKLVNETLYDHVEQMKQIYQNREQLKLLGDYLVLCRSGALKEISKRLDHRHYLLECPHKYSVADLRQVGDLEKRKCFNLEAGGNSVQENNTHFFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZ75</accession>
    <name>A0A6I9YZ75_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable G-protein coupled receptor 156</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GPR156</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930023.1">
        <property type="nucleotide sequence ID" value="XM_014074548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZ75"/>
    <dbReference type="GeneID" id="106555658"/>
    <dbReference type="KEGG" id="tsr:106555658"/>
    <dbReference type="CTD" id="165829"/>
    <dbReference type="OrthoDB" id="411630at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF20">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 156-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="11">
        <location>
            <begin position="207"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013930023.1"/>
        </source>
    </evidence>
    <sequence length="496" mass="56079" checksum="170E62FD34EB8429" modified="2020-10-07" version="1">MSILAEVSGKCCSSFLEGNRWKSCGNGSLERIVFTCTRQSFSARAKPLPPHYLKRAENLDCTPFIETENLISAQIMWLNQGLECAVEMELNYSKLYGNNLDYESQEQQKLILKDLCTSTIHFSETSCKNASSFSVALLAIVWAFNTSGILLSFFLIVFTIRFRKNSSSIHFSVFRICSKHDLGLLVVTRESEVKPIKHNERAINGPWIVKMSSPNLNVVTLLGSGLTYGTVYLFGIEKQNPLSRPSLEMLIQVRICILYISGSFALGPVLGKSWRLYRVFTQKVPDKRVIIKDFQLLVMLSLLVLADIILLLIWISLDPVQCLQNLNVDVKGILLMYGIYLAGLTDNLSCPPINQSLTLISAVAIIFLFIGIMLVVNNFFYLWHNLVYGLTSGGIFVCMTSINCLIFIPQIRQWKTFEKQEIDTSNMAKYFTGSNKNFHSTMYSDEEIYQLLGEKNTMIQQLAEVGNIYIISKKNENTASHHCATILTCYMNIIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZP8</accession>
    <name>A0A6I9YZP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BCL2-associated athanogene 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">BAG6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930193.1">
        <property type="nucleotide sequence ID" value="XM_014074718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZP8"/>
    <dbReference type="GeneID" id="106555804"/>
    <dbReference type="KEGG" id="tsr:106555804"/>
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    <dbReference type="OrthoDB" id="1885901at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01809">
        <property type="entry name" value="Ubl_BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000041">
        <property type="entry name" value="large proline-rich protein BAG6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021925">
        <property type="entry name" value="BAG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204:SF0">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12057">
        <property type="entry name" value="BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="13">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="76"/>
            <end position="114"/>
        </location>
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            <end position="248"/>
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            <end position="642"/>
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            <end position="716"/>
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            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
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            <end position="110"/>
        </location>
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            <end position="227"/>
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        <location>
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            <end position="392"/>
        </location>
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        <location>
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            <end position="402"/>
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    </feature>
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        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="547"/>
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        </location>
    </feature>
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        <location>
            <begin position="585"/>
            <end position="625"/>
        </location>
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            <begin position="702"/>
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        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
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            <position position="716"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030033"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013930193.1"/>
        </source>
    </evidence>
    <sequence length="716" mass="73198" checksum="843034C05A79237F" modified="2020-10-07" version="1">MAELDNLEVMIKTLDSQTRTFTVEGEITVKEFKDHIAGSVNIPSEKQRLIYQGRVLQDDKKLKEYNVGGKVIHLVERAPPQTQSPSGGPSGAGLSSAPHNNTGPRGSGSTVHDRNANSYVMVGTFNLPVSIMDPQPPTQNDGSSVDVHINMDQAPIQSEPRVRLVMAQHMLRDIQGILNRLEGNTSDHSAVGSEDTPSGSPTEREPMTGTESEPPQEPTPAEETPPSGSEPPPGTEAAAPNHPSPGEYVEVLGELRGLESRLQPFLQRYEEVLGTAGTADYNNNTEGREEDQRIINLVGESLRLLGNTFVALSDLRCNLSCSAPRHLHVVRPMSHYTAPMVLQQAAIPIQINVGTTVTMTGNGGRTVQTNLEGSAAAAAAAAAAQAAPTAQPRTSSPVESTQPSEGSPPSHSPGVTPVPPQASQPGHPRVIRISHQTVEPMVMMHMNIQDSSSQLGSGSAGGVPSMGPAGSTGHSQGMGSGAHMPLPSLPPEFMQAVAHQITQQAMAAAASAATGQQVPSFPSAPTRVVIARPSAPSVRPTHAGSPQGVGQGGPGMGGNASLAQMISGLVGQLLMQPVIVAQGSGASSSTSVSSHATFSTSTSASPPTASASAGTTNTATTAAGGSSAGAPGGPMPSGQTPAAATELQFSQLLGNILGAAGSSMAGVAGVGSPTITVAMPGVPAFLQGMTDFLQATQMGGGPPNPPEQMAPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z273</accession>
    <name>A0A6I9Z273_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signaling mucin HKR1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931153.1">
        <property type="nucleotide sequence ID" value="XM_014075678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z273"/>
    <dbReference type="GeneID" id="106556705"/>
    <dbReference type="KEGG" id="tsr:106556705"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931153.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="27069" checksum="728F08F6E189B1D0" modified="2020-10-07" version="1">IAESTSSPSTTSAIIAESTSSPSTTSAIIVESTSSPSTTSAIIAESTSSPSTTSAIIAESTSSPSTTSAIIAESTRSPSTTSAIIAESTSSPPTTSAIIAESTSSPSTTSAIIAESTSSPSTTSAIIVEHTSSPSTTSAIIADSTNSLSTTSAIISESTSSPSTTSAIIVESTSSPSTTSAIIAGSTSSSSTTTAIIAWSTSIPSTTSGIITESTSSPSTTNTTTAKITNSPSIVKTTTAMITNSPSIVKTTTARRSTPSTTKTTAVKSTTRTTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z330</accession>
    <name>A0A6I9Z330_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL66</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRPS18A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial ribosomal protein bS18 family. Mitochondrion-specific ribosomal protein mL66 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930386.1">
        <property type="nucleotide sequence ID" value="XM_014074911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z330"/>
    <dbReference type="GeneID" id="106555964"/>
    <dbReference type="KEGG" id="tsr:106555964"/>
    <dbReference type="CTD" id="55168"/>
    <dbReference type="OrthoDB" id="10054543at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.10:FF:000011">
        <property type="entry name" value="28S ribosomal protein S18a, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF66">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013930386.1"/>
        </source>
    </evidence>
    <sequence length="160" mass="18334" checksum="51F708C8CA9EEC43" modified="2020-10-07" version="1">FVFAVVETTEGNVTTIEGKMIDNVEIPSPPNPSGNCPICRWNLKHKYDYMDVLLLSQFITSTGGMISRKITGLCDEEHRKIAMCVEMAHRAGLLPDHKPKRPEGFVSKPKLNRYHTRYPIECVKPIYKKGRKWCKVPMPISHPLLKDNITYGSKPLRFNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3U0</accession>
    <name>A0A6I9Z3U0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Rab11 family-interacting protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RAB11FIP4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Recycling endosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931763.1">
        <property type="nucleotide sequence ID" value="XM_014076288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3U0"/>
    <dbReference type="GeneID" id="106557136"/>
    <dbReference type="KEGG" id="tsr:106557136"/>
    <dbReference type="CTD" id="84440"/>
    <dbReference type="OrthoDB" id="418358at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2440:FF:000001">
        <property type="entry name" value="RAB11 family interacting protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037245">
        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057316">
        <property type="entry name" value="Rab11-FIP3/4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051977">
        <property type="entry name" value="Rab11-interacting_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726:SF5">
        <property type="entry name" value="RAB11 FAMILY-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726">
        <property type="entry name" value="RAB11-FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25450">
        <property type="entry name" value="Rab11-FIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FIP-RBD" evidence="11">
        <location>
            <begin position="409"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="190"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013931763.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="54452" checksum="26875C1DB6F753AB" modified="2020-10-07" version="1">MLGLCHDVFFLFPPRSNLLNPASSDLSTYSTASLISNEEQFEDYGEGDDVDFTPSSPCLDDESRTHAYSDLGSSVSSSAGQTPRKMRHIYNSELLDVYCTQCCKKINLLNDLEARLKNLKANSPNRKISSTAFGRQLFHNSNFSSSNGSTEDLFRDSIDSCDNDITEKVTYLEKKVSELENDTVTNGDLKSKLKQENTQLVHRVHELEELLKDQETSAQQTLEEEIKRHREAYSKYEKEKSTEIELLNTRVQQLEEENCELKTTVLRLKSQTEKLDEERQRMSDRLEDTSLRLKDEMDVYKRAGEKEYKIRQRSQKELAAGLTQGSIVLCFFLQLIEDLRKELEHLQLYKLDCERPGRGRSSSSLSEFNAKTREVEMEHEIKRLKQENQKLHDQNDDLNGQILSLSLYEAKNLFATQTKAQSLAAEIDSASRDELMEALKEQEEINYRLRQYMDKIILAILDHNPSILEIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z420</accession>
    <name>A0A6I9Z420_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dedicator of cytokinesis protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DOCK7</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931402.1">
        <property type="nucleotide sequence ID" value="XM_014075927.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106556888"/>
    <dbReference type="KEGG" id="tsr:106556888"/>
    <dbReference type="CTD" id="85440"/>
    <dbReference type="OrthoDB" id="47328at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08696">
        <property type="entry name" value="C2_Dock-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000022">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037808">
        <property type="entry name" value="C2_Dock-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF78">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="561"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1624"/>
            <end position="1792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="888"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="935"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="888"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="943"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013931402.1"/>
        </source>
    </evidence>
    <sequence length="1792" mass="202194" checksum="869958ECCB85F700" modified="2020-10-07" version="1">MAERRAFAQKISRTVAAEVRKQISGQYGGSPQLLKNLNIGGTISHHTSVPLTEPVDPVDLEDYLLTHPLAVDSGPMRDLFEFPSDDIEVIYSPRECRTLVSAVPEESEMDPHVRDCVRSYTEDWAIVNRKYHKLGTGFNPNTLDKQKERQKGLPKQMFESDEAPDGNSYQDEQDDLKRRSMSIDDTPRGSWACSIFDLKNSLPDALLSNLLDRTPNEEIDHQNDEQRKSNRHKELFALHPAPDEEEPIERLGIPEIPKEHFGQRLLVKCLSLKFEIEIEPIFASLALYDVKEKKKISENFFFDLNSEQMKSMLRPHVPPAAITTLARSAIFSITYPSQDVFLVIKLEKVLQQGDIGECAEPYMIMKEADAAKNKEKLDKLKSQSEQFCQRLGKYRMPFAWTAIHLMNIVSSAGSLERDSAEVEIATGERKGSWSERRNSSIVGRRSLERTTSGDEACNLTNFRPATLTVTNFFKQEGDRLSDEDLYKFLADMKRPSSVLRRLRPITAQLKIDISPAPENPHYCLTPELLQVKLYPDSRVRPTREILEFPARDVYIPNTTYRNLLYVYPQSLNFANRQGSARNITVKVQFMFGEDPSNAMPVIFGKSSCAEFSKEAYTAVVYHNRSPDFHEEIKIKLPATLTDHHHLLFTFYHVSCQQKQNTPLETPVGYTWIPMLQNGRLKTGQFCLPVSLEKPPQAYSVLSPEVPLPGMKWVDNHKGVFNVEIIAVSTIHTQDPYLDKFFALVHALDEHMFPVRIGDMRIMENNLENELKSSILALNSSQLEPVVRFLHLLLDKLILLVVRPPIIAGQIVNLGQASFEAMASIVNRLHKNLEGNQDQHGRNNLLTSYIYYVFRLPNMSPNSPCPAPSGLGGSVHYATMARSAVRPASLNLNRSRSLSNSNPDISGTPTSPDDEVRSIIGSKGLDRSNSWVNTGAAKAAPWGSNPSPNAETSQAADRSCNRMSSHTETPSFLQTLTGRLPTKKLFHEELALQWVVCSGSVREAALQQAWFFFELMVKSIVHHLYFADKLDASRKNRFPERFMDDIGALVSTIASDIVSRFQKDTEMIERLNTSLAFFLNDLLSVMDRGFVFSLVKTYYKQLRDRTPATEVRLLQDISKVLAATQFSADATLDAVQYAFRALSSSVATRRLLWLCHWQADLFGLHKKVINMVHNLLSSHDSDPRYSDPQVKARVATLYLPLIGVIMESVPQLYDFTESHNQRGRPSCVAVEDCESEGGNMISQTVAMAIAGTPVPQLTRPTSFLLTSTGSRQHSTFSADSSRNLLICLLWVLKNADESVLQKWFTDLSVLQLNRLLDLLYLCISCFEYKGKKVFERMNSLTFKKSKDMRAKLEEAILGSIGARQEMVRRSRGQLERSPSGSAFGSQENLRWRKDMTHWRQNTEKLDKSRAEIEHEALIDGNLATEANLIILDTLEIIVQTVSVTESKESILGGVLKALLHSMACNQSALYLQHCFATQRALVSKFPELLFEEETEQCADLCLRLLRHCSSSISTIRSHASASLYLLMRQNFEIGNNFARVKMQVTMSLSSLVGTSQNFNEEFLRRSLKTILTYAEEDLELKETTFPDQVQDLVFNLHMILSDTVKMKEHQEDPEMLIDLMYRIAKGYQNSPDLRLTWLQNMAGKHSARNNHAESAQCLVHSAALVAEYLSMLEDRKYLPVGCVTFQNVSSNVLEESAVSDDVVSPDEEGICSGKYFTEAGLVGLLEQAAACFSMAGMYEAVNEVYKILIPIHEANRDANKLATIHGKLQDAFSKIVHQSTGWEVGNVLASKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z473</accession>
    <name>A0A6I9Z473_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106556931</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013931452.1">
        <property type="nucleotide sequence ID" value="XM_014075977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z473"/>
    <dbReference type="GeneID" id="106556931"/>
    <dbReference type="KEGG" id="tsr:106556931"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05582">
        <property type="entry name" value="STKc_RSK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000010">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000041">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000013">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016239">
        <property type="entry name" value="Ribosomal_S6_kinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041906">
        <property type="entry name" value="RSK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000606">
        <property type="entry name" value="Ribsml_S6_kin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="59"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="17">
        <location>
            <begin position="319"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="373"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="65"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000606-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000606-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013931452.1"/>
        </source>
    </evidence>
    <sequence length="691" mass="78632" checksum="07FFECD16B1E703C" modified="2020-10-07" version="1">METNMKKFTVRRFFSVYLRKKSRSKSSSLRSFEEESIVKEIDISHHVKEGFEKADPSQFELLKVLGQGSYGKVFLVRKIKGSDTGQLYAMKVLKKATLKVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHGLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKLGMPQFLSIDAQSLLRALFKRNPSNRLGAGLDGVEEIKRHPFFATIDWNKLYRREIKPPFKPAVGRPEDTFHFDPEFTSRTPTDSPGVPPSANAHHLFRGFSFVASNLGQELVQQEPHKVTVHPIVQIIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKFVYLVMELMRGGELLDRILRQKCFSEREASAVLCTISKTVDYLHSQGVVHRDLKPSNILYVDESGNPESIRMCDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGKYALAGGNWDSISDAAKDIVSKMLHVDPHQRLTATQVLRHPWIVNREYLSQNQLSRQDVHLVKGAMAATYFALNQTPQAPRLEPVLSSNLAQRRGMKRLTSTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4T8</accession>
    <name>A0A6I9Z4T8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tumor protein 63-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106556404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930875.1">
        <property type="nucleotide sequence ID" value="XM_014075400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4T8"/>
    <dbReference type="GeneID" id="106556404"/>
    <dbReference type="KEGG" id="tsr:106556404"/>
    <dbReference type="OrthoDB" id="5915660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002117">
        <property type="entry name" value="p53_tumour_suppressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11447">
        <property type="entry name" value="CELLULAR TUMOR ANTIGEN P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11447:SF8">
        <property type="entry name" value="TUMOR PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013930875.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="11738" checksum="CFDFCC8B9044C4C9" modified="2020-10-07" version="1">MRSDLVSLKIPEQFRHAIWKGILDYRQLHDFSSPPHLLRTPSGASTVSVGSSEARGERVIDAVRFTLRQTISFPPRDDWNDFNFDLDARRNKQQRIKEEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z560</accession>
    <name>A0A6I9Z560_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mulatexin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931000.1">
        <property type="nucleotide sequence ID" value="XM_014075525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z560"/>
    <dbReference type="GeneID" id="106556540"/>
    <dbReference type="KEGG" id="tsr:106556540"/>
    <dbReference type="OrthoDB" id="9909819at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931000.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="15280" checksum="D98FD0A1A6315B21" modified="2020-10-07" version="1">MLSAFDHFHGDKPFPLHFPSTTATTTVGPVSADVCNKGETRAFSLDRGLGRREGQAVCFGCESFLIFPEIKQKIELLMSVNSEKSTSSERPEPQQKVPSAPPPPPPPPPPPLPEPTPPEPEEEILGSDDEEQEDPADYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5B6</accession>
    <name>A0A6I9Z5B6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Growth/differentiation factor 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931156.1">
        <property type="nucleotide sequence ID" value="XM_014075681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5B6"/>
    <dbReference type="GeneID" id="106556708"/>
    <dbReference type="KEGG" id="tsr:106556708"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026786114" evidence="2">
        <location>
            <begin position="20"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931156.1"/>
        </source>
    </evidence>
    <sequence length="116" mass="12289" checksum="1EF10CD31FB8890C" modified="2020-10-07" version="1">MNAARALLSAVFLAGFFWGDLPCCQQATIPAAATAAPSLREPGKGKRSCREGGTSRSAGEAAKKGSAVAQPRVEPHEYMLSLYRTYSIAEKLGTNASFFQSSKAANTITSFVDRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5U5</accession>
    <name>A0A6I9Z5U5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106557344</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013932037.1">
        <property type="nucleotide sequence ID" value="XM_014076562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5U5"/>
    <dbReference type="GeneID" id="106557344"/>
    <dbReference type="KEGG" id="tsr:106557344"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd05738">
        <property type="entry name" value="IgI_2_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05739">
        <property type="entry name" value="IgI_3_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000027">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000036">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000066">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000068">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000015">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000082">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000098">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase F isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF8">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="107"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="196"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="291"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="389"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="486"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="588"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="697"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013932037.1"/>
        </source>
    </evidence>
    <sequence length="774" mass="85461" checksum="E41DB5B4D980B7BD" modified="2020-10-07" version="1">MGPQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDPSASNGRIKQLRSDTFEGTPIRGGLQIESSEETDQGKYECVASNSAGVRYSSPANLYVRELREVRRVAPRFSIPPTSHEIMPGGNVNITCVAVGSPMPYVKWMQGAEDLTPEDDMPVGRNVLELTDVKDSANYTCVAMSSLGVIEAVAQITVKSFPKAPGTPVVTETTATSITITWDSGNPDPVSYYVIEYKSKSQDGPYQIKEDITTTRYSIGGLSPNSEYEIWVSAVNTIGQGPHSDSVVTRTGEQAPASAPRNVQGRMLSASTMIIQWEEPVEPNGQIRGYRVYYTMEPDQPVSNWQKHNVDDSLLTTVGSLLEHETYTVRVLAFTSVGDGPLSDPIQVKTQQGVPGQPMNFRAEAKSESTILLTWSPPRQDIILKYELLYKEVSNKPGTPEFLKTFDPVTFFLVKELNPNTEYVFQLAARSTLGLGAPTSEVRERTLQSKPSAPPQDVKCVSTRSTAILVSWQPPPVESQNGDLEGYSVRYRGLDPEVSELKEVKDIPATTTQISLDSLEKWTEYRITVVAHTIMGPGPESSPIIVRTDEDVPSAPPRKVEVEVLNSTAIQVFWRSPIQNRQHGQIRGYQVHYVRMENGEASGLPQIKDVMLADAQWETDDTAEYEMVISGLQPETTYSVTVAAYTMKGDGARSKPKQVMTKGAVPGKPILSVYPTHENSLLVKWEPPVEAESQVLGYRLQFGRKDVDPLATLEFATQEDKYTATHVHKGATYVFKLAVKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0Z7</accession>
    <name>A0A6I9X0Z7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox protein OTX2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106538819</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908919.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0Z7"/>
    <dbReference type="GeneID" id="106538819"/>
    <dbReference type="KEGG" id="tsr:106538819"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN OTX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="36"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="38"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908919.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13839" checksum="6ABE532A46EC55B1" modified="2020-10-07" version="1">MMSYLKQPPYAVNGLSLTTSGMDLLHPSVGYPATPRKQRRERTTFTRAQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVREGLVRAPPPFTFSSGSPGSWGGGEEMGGRTIQLVRVWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X138</accession>
    <name>A0A6I9X138_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MED1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908959.1">
        <property type="nucleotide sequence ID" value="XM_014053484.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106538856"/>
    <dbReference type="KEGG" id="tsr:106538856"/>
    <dbReference type="CTD" id="5469"/>
    <dbReference type="OrthoDB" id="2281547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042974">
        <property type="term" value="F:nuclear retinoic acid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046966">
        <property type="term" value="F:nuclear thyroid hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042809">
        <property type="term" value="F:nuclear vitamin D receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097067">
        <property type="term" value="P:cellular response to thyroid hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051999">
        <property type="entry name" value="Mediator_complex_subunit_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881:SF10">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="11">
        <location>
            <begin position="62"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="619"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="667"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="797"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="950"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="667"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="812"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="851"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="966"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1038"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1086"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1102"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1188"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1228"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1245"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1344"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1363"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1435"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1459"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1507"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1517"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1544"/>
            <end position="1563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013908959.1"/>
        </source>
    </evidence>
    <sequence length="1592" mass="169494" checksum="56DFBCD217A23E43" modified="2020-10-07" version="1">MKAVQGNTEESEKLNKMSSLLERLHVKFNPNSRPWTETMKLVRQVMEKRIVMNSGGHQNLITCLETLQKALKVSSLPAMTDRLESIARQNGLGSHLSANGTECYITSDMFYVEVHLDTTGQLRDVKVAHHGENPVSCPELVQHLRERNFNEFSKHLKGLVNLYKVPGDNKLKTKMYLALQSLELDLSKMAGMYWQATNASPLDKILHGSVGYLTPRSGGHLMNLKYYVSPYDLFEEGTGAPIILNENNVPRYLGMNVCVTIEGTLTMNKLPIAPLIMGTHPVDNKGTPSFSSVTSANSVDFPACFFLKFPKPVPVSQAFIQKLQNCTGIPLFDTPPTYVPLYELITQFELSKEEEEEDPCPLHNTMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLLNKIAFHHPSRVPLILSMIRHQVAYNTLIGSCVKRTVLKEDSPGILQFEVCPLSDSCFSVSFQHPVNDSLVCVVMDVQDSTHVNCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTSTFPGGPISTLFSMSMGIKERHDSVGHGEDFSKVSQNPILTSLLQITGNVGSTIGSSPTPPHHTPPPVSSPASNTKNHPMLMNLLKDNAAQDFSTLYGSSPLERQNSSSGSPRMEMGPGGNKQKKKKSRVLVDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTTDTLDTPHITPAPSQCSTPPTTYPQSIPHAQPSIQRMVRLSSSDSIGADVTDILSDIAEEASKLPNTNEDCPPIGTPIRDSSSSGHSQSALFDPDVFQSNNSENPYTDPADLIADATVSPNSDSSNQFFPDGVDFNPDLLNSQSQSGFGEDYFDDSSQSGDADDFKGFAPQTISTLGVQVLGGDGGESKFKASSQADTVDFSIITATSKALGASDVLEHHSGSQSPLLSMGELGKDKSQKRVKEGNGSGSSLTGPGLDGKGGKRNRTPSSDGKSKEKVQKRRKVEPEGKSPSHSTSTRPFTPPASTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVTVGKPSHGQYTSSGSVSSLSSKSHHSHSSSSSSSSSSSSSSSSSSSTSGKIKSSKSDGSSGSKMSGSLYSGQGSSGSGQSKSSTQSVGKPGSSPITKHGLSSGSGGTKIKPQGKPSSLMNPSMSKPNISPSHSRPSGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHIPGTGSSSSMKSSSGMGSSSSMSQKPPPSSNSSMGSSSSFSSGSSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPTGEDSVDGQMVQNSSSSGHSMSSKHNLSGNELQGKRERSDKEKSKIPASGGSSESSKKAPDSKSVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGEGSGDGLRPQISSSKNYGSPLISGSTPKHERCSPSHSKSPAYTPQNIDSESESGSSIAEKSYQNSPNSDDGVRPLPEYSSEKHKKHKKEKKKLKDKDRDRDRDKERDKKKSHSIKPESWSKSPISSEQSLSMTSSAILTSDRPSRPSPDFLIGEEDDDLMDVALIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1W9</accession>
    <name>A0A6I9X1W9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Type II GnRH receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106539091</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909249.1">
        <property type="nucleotide sequence ID" value="XM_014053774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1W9"/>
    <dbReference type="GeneID" id="106539091"/>
    <dbReference type="KEGG" id="tsr:106539091"/>
    <dbReference type="OrthoDB" id="6022667at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000199">
        <property type="entry name" value="Gonadotropin-releasing hormone II receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001658">
        <property type="entry name" value="GphnRH_fam_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF69">
        <property type="entry name" value="GONADOTROPIN-RELEASING HORMONE II RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00529">
        <property type="entry name" value="GNADOTRPHINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="36"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909249.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="44285" checksum="EB3A8133E9991C85" modified="2020-10-07" version="1">MEHNRSGLWGRGSSFKGLEESVVLPIFSVAAQVRVGLTLLLCVISATCNMAMLWTGWSRPAAKGSHARLLLLHLAVADLLVALVVMPLDAAWNITLQWQAGDVACRLLMFLKLLAMYASAFITTLISLDCQAAILQPLATARAWRKNQILLYAAWLMSASLSAPQLFLFHTVTISSPQNFTQCTTHGSFAQHWHEVAYNMFGFLGLFLLPLLVMSACYTRVLLEISRHLSSCNSWSAQETPLRRSRNPIPRARLRLLHLSLAIVGSFVLCWTPYYLLGLWYWFWPVDMEGTVSPSLAHILFLFGLLNACLDPITYRLFAGGWQGSVFYLVLKKLLSFFHLVLKKLLNFFYLALKTLLSFFHMVLKKLLSFFYMVLKVLLSFFYLVLKKLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2I2</accession>
    <name>A0A6I9X2I2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Double-stranded RNA-specific editase B2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ADARB2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908037.1">
        <property type="nucleotide sequence ID" value="XM_014052562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2I2"/>
    <dbReference type="GeneID" id="106538143"/>
    <dbReference type="KEGG" id="tsr:106538143"/>
    <dbReference type="CTD" id="105"/>
    <dbReference type="OrthoDB" id="10268011at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19896">
        <property type="entry name" value="DSRM_RED2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000009">
        <property type="entry name" value="Adenosine deaminase RNA-specific B2 (inactive)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000011">
        <property type="entry name" value="double-stranded RNA-specific editase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044460">
        <property type="entry name" value="ADAR3_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF17">
        <property type="entry name" value="DOUBLE-STRANDED RNA-SPECIFIC EDITASE B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DRBM" evidence="10">
        <location>
            <begin position="170"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="10">
        <location>
            <begin position="328"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="A to I editase" evidence="11">
        <location>
            <begin position="459"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013908037.1"/>
        </source>
    </evidence>
    <sequence length="790" mass="88366" checksum="0A674C91AA2D008A" modified="2020-10-07" version="1">MTSVLESSRTSGGAGGQLKGKSKRRRRRRSKRKAENSGWVHTLLPRISHRLQERCNTQFGTWLDATDHRLSTQSETPHEKGSILSTFIAPLKYLNPSAANMENEENLNISSAEVKENRNIGNLEDHSVASNERSRVAASRVKRKRPLEEGNRGHFCKFQLICKKISWTVTPKNALVQLHELKPGLQYKMISQTGPVHAPVFAVAVEVNGHTFEGTGPTKKKAKMRAAEQALKSFVQFPNASQAHFAMGNCFNPSTDFTSDLEDFPDTLFKEFEPSTHSKEFSVCAPVNNELLSKACRQGKLLYHTLGFMGHPKQNKHKMAPTVKSEKNPVVLLNELRSGLRYVCLSETVEKQHTKRFVMAVRVDGRTFEGSGRSKKLAKGQAAQAALQALFNIRLPSQIPSRRKCNHLPQEFADSVYQIITEKFQEVTDNFAALHACHRSLAGIVMTRGMDIQQAQVIVLSSGTKCLNGEYLNDQGLVVNDCHAEIVARRAFIHFLYSQLELHLSRQQEDSERSIFVRLKEGGYRLKENILFHLYVSTSPCGDARLNSPYEITSDLNSVKHIVRKYRGHLRTKIESGEGTIPARCTNGIQTWDGVLLGEQLITMSCTDKIARWNVLGLQGALLSYFIEPVYLHSIIVGSLSHTGHLSRVMSHRLEDVGQLPASYRCNQLLLSGVSNAERRKAGKSPGFSVNWIAGTADLELINATTGKKNCGSCSRLCKHMFYTQWAKLYGKLSTPISSHGEVPSVYSEAKLVAHVYQSAKQQLFRAFQKAGLGTWVKKPPEQDQFLLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X303</accession>
    <name>A0A6I9X303_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein boule-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">BOLL</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with DAZ1 and DAZL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908217.1">
        <property type="nucleotide sequence ID" value="XM_014052742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X303"/>
    <dbReference type="GeneID" id="106538294"/>
    <dbReference type="KEGG" id="tsr:106538294"/>
    <dbReference type="CTD" id="66037"/>
    <dbReference type="OrthoDB" id="762982at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070935">
        <property type="term" value="P:3'-UTR-mediated mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12673">
        <property type="entry name" value="RRM_BOULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000167">
        <property type="entry name" value="protein boule-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043628">
        <property type="entry name" value="DAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176:SF10">
        <property type="entry name" value="PROTEIN BOULE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51890">
        <property type="entry name" value="DAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="33"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="DAZ" evidence="14">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="285"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="315"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013908217.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="36439" checksum="4B4369C43181C746" modified="2020-10-07" version="1">MQTDSLSPSPKTVSLVPLNNTTSAPRFGTVIPNRIFVGGIDFKTNENDLRKFFSQYGCVKEVKIVNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPQPWSRSISSSPVMLAQPIYQQPTYHYQAPAQCLPGQWQWSIPQSPASPTSFFYLHPSEITYQPLEIAQDGGCVPPPLSLMEAAVPEPYTDHGVQAAYHQVYAPSAIAMPAPVMQSETIKESRIHSVRRNFSHPSSMSLKPRYARTPHFHPRKDYRSDDSMLNTSSSTDSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3Z8</accession>
    <name>A0A6I9X3Z8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Recombining binding protein suppressor of hairless-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RBPJL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Su(H) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910029.1">
        <property type="nucleotide sequence ID" value="XM_014054554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3Z8"/>
    <dbReference type="GeneID" id="106539704"/>
    <dbReference type="KEGG" id="tsr:106539704"/>
    <dbReference type="CTD" id="11317"/>
    <dbReference type="OrthoDB" id="5600360at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000003">
        <property type="entry name" value="recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000074">
        <property type="entry name" value="Recombining binding protein suppressor of hairless, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015350">
        <property type="entry name" value="Beta-trefoil_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036358">
        <property type="entry name" value="BTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040159">
        <property type="entry name" value="CLS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015351">
        <property type="entry name" value="RBP-J/Cbf11/Cbf12_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038007">
        <property type="entry name" value="RBP-Jkappa_IPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10665">
        <property type="entry name" value="RECOMBINING BINDING PROTEIN SUPPRESSOR OF HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09270">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09271">
        <property type="entry name" value="LAG1-DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20144">
        <property type="entry name" value="TIG_SUH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01268">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01267">
        <property type="entry name" value="LAG1_DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110217">
        <property type="entry name" value="DNA-binding protein LAG-1 (CSL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RBP-J/Cbf11/Cbf12 DNA binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-trefoil DNA-binding" evidence="8">
        <location>
            <begin position="72"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013910029.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="43243" checksum="42C03B7A4D6DCB85" modified="2020-10-07" version="1">MQKLNFEEQLDCKKFSCAKALYISDSDKRKHFRLALKLFFSNGQEIGTFHSKLIKVISKPSQKKQSLKNTDLCILSGSKVSLFNRLRSQTVSTRYLFVEDDAFTASARQWAAFTLHLDEFCSQSQGEFPLREAYIRYGSRVRLICTTTGLALPPLIIRKVAKQLVMLDVDEPVSQLHKCAFQFHGSDRMYLCLSTEKVIQYQASPCPKEINRELLNDSSCWTIIGTEAVEYSFSLNPTSIQNPIGPVPLITTLELTGGGDVAMLEVQGNYFHANMKIWFGDVEAETMYRNSKSLVCVVPDISAFRPDWRWLRYPITVPLLLLRNDGPIYSSIFSFTYTPEQGFLCGQPLLPEMVQDSDTLLNTIHQEFTKTNYHLFMQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6M2</accession>
    <name>A0A6I9X6M2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Myb-related protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MYBL1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911109.1">
        <property type="nucleotide sequence ID" value="XM_014055634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6M2"/>
    <dbReference type="GeneID" id="106540508"/>
    <dbReference type="KEGG" id="tsr:106540508"/>
    <dbReference type="CTD" id="4603"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000042">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015395">
        <property type="entry name" value="C-myb_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012642">
        <property type="entry name" value="Tscrpt_reg_Wos2-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF9">
        <property type="entry name" value="MYB-RELATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09316">
        <property type="entry name" value="Cmyb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07988">
        <property type="entry name" value="LMSTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="82"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="82"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="134"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="138"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013911109.1"/>
        </source>
    </evidence>
    <sequence length="743" mass="85268" checksum="5139B2FA4BE2184F" modified="2020-10-07" version="1">MAKRPRSEEDDDELHYADHDYEVPQQKGAKKICNRVKWTRDEDEKLKKLVEQHGTSDWTFISSHLQNRSDFQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSLIAKHLKGRIGKQCRERWHNHLNPEVKKSSWTEEEDRIIYEAHKRLGNRWAEIAKLLPGRTDNSIKNHWNSTMRRKVEQEGYLQDGIKTERPCLSKLPHKTCVSTDHLHAQNQFYIPVQTHIPGYQYVSTESSCLDVAAPSHFIQPFDDDPDKEKKIKELELLLMSAENEIRRKRVSSQARNFSNWSGNFIMEDNMANTLNSLGEQTNDFYDIDDTQASINQQTSPSKYLAVEANAVLSSLQTIPEFAETLELIESDPVAWSDVTSFDLSDTAPSPVNQAPVKLMRIQHNDGAMECQFNVSVVLDGKKIRDEEESRSPNVAKFSTPTILRKKKKIRTGQSSAYEQNDGLCSNNTMLKQTPVKTLPFSPSQFFNTCSGNEQFNLENTPFTSTPICSQKVLITTPIHNKDVTPKDQKENAGFRTPTVRRSLLGTTPRTPTPFKNALAAQEKKYGPLKIVSQPLAFLEEDIREVLKEETGTDIFFKEEEDSAHKECKLENTSVKNVRKSLVLDPWEKEELGDPFLAEDDVSDMQSENIYTTSLLMIPLLEIHDNRDNTTPEKQDSSSTKLNIAWKKKLNTYTSNNIKAEKPLQVNCEWEAVVYGKTEDQLIMTEQARRYLSTYSASGSTSRALIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7N5</accession>
    <name>A0A6I9X7N5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger protein 385D isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106537719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907388.1">
        <property type="nucleotide sequence ID" value="XM_014051913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7N5"/>
    <dbReference type="GeneID" id="106537719"/>
    <dbReference type="KEGG" id="tsr:106537719"/>
    <dbReference type="OrthoDB" id="9448812at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000276">
        <property type="entry name" value="zinc finger protein 385A isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000121">
        <property type="entry name" value="zinc finger protein 385B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000293">
        <property type="entry name" value="zinc finger protein 385B isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051845">
        <property type="entry name" value="Znf385"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 385D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013907388.1"/>
        </source>
    </evidence>
    <sequence length="395" mass="42629" checksum="646F1700BE213E45" modified="2020-10-07" version="1">MRNIMYFGGTCQSPALPTLVRSSIPSLPPSLDLKPFLPFPLDTAAAVNLFPNFNAMDPIQKAVINHTFGVPLPHRRKQIISCNICQLRFNSDSQAAAHYKGTKHAKKLKALEAMKHKQKCVTTKDSAKTIFTSITTNTINTNSDKTDSTTEAPAISTSPAIEIRKNSVIMTEITSKIEKFPSTATSSSVSSLGETEEEKAKRLLYCSLCKVAVNSASQLEAHNSGTKHKTMLEARNGSGNIKAFPRTGVKGKGPINKGNTGLQNKTFHCEICDVHVNSETQLKQHISSRRHKDRAAGKPPKPKYSPYNKLQKGTHPLGVKLVFSKEAAKSVAPRILPNPLAAAAAAAAVAVNSPFSLRTAPPATLFQTSALPPSLLRPAPGPIRTTHTPVLFAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7R0</accession>
    <name>A0A6I9X7R0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCT-eta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CCT7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911519.1">
        <property type="nucleotide sequence ID" value="XM_014056044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7R0"/>
    <dbReference type="GeneID" id="106540794"/>
    <dbReference type="KEGG" id="tsr:106540794"/>
    <dbReference type="CTD" id="10574"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013911519.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="45190" checksum="2DC3826EE7F9B7AA" modified="2020-10-07" version="1">MMPTPVILLKEGTDTSQGIPQLVSNISACQVIAEAVRTTLGPRGMDKLIVDDRGKTTISNDGATILKLLDVVHPAAKTLVDIAKSQDAEFWPLVLKRVNHPCPVPNLVHDLHQGFLWGPLVVDDPSLLPLVPQDYQAIVDAEWNILYDKLEKLHKSGAKVILSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQSSANALTDNVLGRCLLFEEAQIGGERYNFFTGCPKAKTCTIILRGGAQQFMDETERSLHDAIMIVRRAIKNDSVVGGGGAIEMEISKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNAGFDATNILNKLRAKHAQGGTWYGVDVNNEDIADNFDACVWEPAVVRINALTAASEAACLIISVDETIKNPRSTVDAPPMGRGRGRPHTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9A2</accession>
    <name>A0A6I9X9A2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Male-enhanced antigen 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MEA1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play an important role in spermatogenesis and/or testis development.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907843.1">
        <property type="nucleotide sequence ID" value="XM_014052368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9A2"/>
    <dbReference type="GeneID" id="106538012"/>
    <dbReference type="KEGG" id="tsr:106538012"/>
    <dbReference type="CTD" id="4201"/>
    <dbReference type="OrthoDB" id="5593200at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009685">
        <property type="entry name" value="MEA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17005">
        <property type="entry name" value="MALE-ENHANCED ANTIGEN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06910">
        <property type="entry name" value="MEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0744">Spermatogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013907843.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="20327" checksum="47E804E9838B4551" modified="2020-10-07" version="1">MAVETVVTQRMGPERICPNEHEQLGLQEPSDAILDPVGEWSSEEPEDEEDENINNGYVYQPLNQELDQGSTTHESSATSTEPALDINQRLQAMRLHLPDPPIESDNEDEERLVSQSSHSSIPMDPEHVELVKRTMAGVKLPTLSIPAWANEISDEQWQDMVQRTLQDRQSQNGFKPEWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBA7</accession>
    <name>A0A6I9XBA7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sodium-dependent phosphate transport protein 2B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Na(+)-dependent phosphate cotransporter 2B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Sodium/phosphate cotransporter 2B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Solute carrier family 34 member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SLC34A2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Involved in actively transporting phosphate into cells via Na(+) cotransport.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>3 Na(+)(out) + phosphate(out) = 3 Na(+)(in) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71255"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:71256"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC34A transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911576.1">
        <property type="nucleotide sequence ID" value="XM_014056101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBA7"/>
    <dbReference type="GeneID" id="106540838"/>
    <dbReference type="KEGG" id="tsr:106540838"/>
    <dbReference type="CTD" id="10568"/>
    <dbReference type="OrthoDB" id="76259at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005903">
        <property type="term" value="C:brush border"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005436">
        <property type="term" value="F:sodium:phosphate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044341">
        <property type="term" value="P:sodium-dependent phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003841">
        <property type="entry name" value="Na/Pi_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01013">
        <property type="entry name" value="2a58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037997">
        <property type="entry name" value="Na_Pi_symport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010:SF23">
        <property type="entry name" value="SODIUM-DEPENDENT PHOSPHATE TRANSPORT PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 34 SODIUM PHOSPHATE , MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02690">
        <property type="entry name" value="Na_Pi_cotrans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="380"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="543"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013911576.1"/>
        </source>
    </evidence>
    <sequence length="692" mass="75669" checksum="CC413F9E1C0D8F64" modified="2020-10-07" version="1">MTAEGSILIFLSDPNVFQDMAPWPELEDAETNKYIEDPSSKPKKTMSDKQGKDTKDGVISMGNQVELLPAYSTTALTAASEEEEEDPWKLPELQDTGVKWSELDGKHKVLYVLRGAVKFILLLGLLYFFVCSLDVLSSAFQLVGGKAAGDIFKDGSVLSNPVAGLVIGVLVTVMVQSSSTSSSIIVSMVSSSLLSVKHAIPIIMGANIGTSVTNTIVALMQAGDRNEFRRAFAGATVHDFFNWLSVFVLLPIEVASQYLYRLTSLIVDSFQLESGENAPELLKVITDPFTKLIIQLDKSVINAIATGDESAKNKSLVKNWCKTTKNVVEMNTTVPGPENCTSSGLCWTSGNMTWTLKNVTETQNLEKCKHIFVDSQLQDLAVGLILLALSLLVLCTCLVLIVKLLNSVLKGQVANVIKKTLNTDFPFPFSWVTGYLAMLVGAGMTFIVQSSSVFTSAITPLVGIGVISIERAYPLTLGSNIGTTTTAILAAMASPGNRLKYSLQIALCHFFFNISGIILWYPVPFMRIPIRLSKGLGNITAKYRWFAIFYLLTCFFLIPLAVFGLSVAGWPYLVGVAVPVFLVLIFVLTVHLLQKKKPHLLPEKLRSWDFLPRWMHSLRPWDDAITAAGGVCGRYCCCCCKRCRNKEADPSEETKDTKALEVHENPVSLADEENCLPKAAKAEKADLSTTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDK4</accession>
    <name>A0A6I9XDK4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Beta-soluble NSF attachment protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NAPB</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911947.1">
        <property type="nucleotide sequence ID" value="XM_014056472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDK4"/>
    <dbReference type="GeneID" id="106541132"/>
    <dbReference type="KEGG" id="tsr:106541132"/>
    <dbReference type="CTD" id="63908"/>
    <dbReference type="OrthoDB" id="9984275at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070044">
        <property type="term" value="C:synaptobrevin 2-SNAP-25-syntaxin-1a complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010807">
        <property type="term" value="P:regulation of synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000028">
        <property type="entry name" value="beta-soluble NSF attachment protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF12">
        <property type="entry name" value="BETA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911947.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="33708" checksum="0FAABB4201FB0862" modified="2020-10-07" version="1">MDASTAGNKEREAMQLLAEAEKRVKSSHSFLRGFFGGNSKLEEACEMYIRAANMFKIAKNWTAAGNAFCQAAKLHMQLQSKHDSATCFVDAGNAYKKADPQEAINCLNAAIDIYTDMGRFTIAAKHHITIAEIYESELVDIEKAVAHYEQSADYYKGEESNSSANKCLLKVAIYAAQLEQYPKAVEIFEQIATSTMDNPLLKHSAKDYFFKAALCHFIVDELNAKLALEKYEEMFPAFTDSRECKLLKKLLEAHEEQNCDAYTEAVKEFDSISRLDQWLTTMLLRIKKSIQGETEGDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDZ7</accession>
    <name>A0A6I9XDZ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase- like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912092.1">
        <property type="nucleotide sequence ID" value="XM_014056617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDZ7"/>
    <dbReference type="GeneID" id="106541237"/>
    <dbReference type="KEGG" id="tsr:106541237"/>
    <dbReference type="OrthoDB" id="188713at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912092.1"/>
        </source>
    </evidence>
    <sequence length="84" mass="9501" checksum="268CAEDC2076B791" modified="2020-10-07" version="1">MMRFLPSYQAVEYMRLGLDPTMACQKVISRIQKYHPYFFGAVICANTSGSYGAACNRVPGFTQFHFMASNPILKQPSEQIIDCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEI9</accession>
    <name>A0A6I9XEI9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Complement C1q-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">C1QL3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912606.1">
        <property type="nucleotide sequence ID" value="XM_014057131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEI9"/>
    <dbReference type="GeneID" id="106541629"/>
    <dbReference type="KEGG" id="tsr:106541629"/>
    <dbReference type="CTD" id="389941"/>
    <dbReference type="OrthoDB" id="10070467at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF96">
        <property type="entry name" value="COMPLEMENT C1Q-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026741765" evidence="6">
        <location>
            <begin position="21"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="7">
        <location>
            <begin position="122"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013912606.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="26715" checksum="AB7F4295CBF17ABE" modified="2020-10-07" version="1">MVLLLVILIPVLVSSAGTSAHYEMLGTCRMVCDPYGGTKVPSTAATPDRGLMQSLPTFIQGPKGEAGRPGKAGPRGPPGEPGPPGPVGPPGEKGEPGRQGLPGPPGAPGLNAAGAISAATYSTVPKIAFYAGLKRQHEGYEVLKFDDVVTNLGNHYDPTTGKFTCSIPGIYFFTYHVLMRGGDGTSMWADLCKNNQVRASAIAQDADQNYDYASNSVVLHLEPGDEVYIKLDGGKAHGGNNNKYSTFSGFIIYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFB6</accession>
    <name>A0A6I9XFB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106539473</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909748.1">
        <property type="nucleotide sequence ID" value="XM_014054273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFB6"/>
    <dbReference type="GeneID" id="106539473"/>
    <dbReference type="KEGG" id="tsr:106539473"/>
    <dbReference type="OrthoDB" id="9445499at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000013">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF227">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909748.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="35858" checksum="7218B85259108CCD" modified="2020-10-07" version="1">MELANGTVAQGFILLGFGMDQQKRFLLLLFLITLYVLTLVENSTIITLVCRDSHLGQVPMYILLRNFSWLEMCYVTTTIPCMLFDLATHNQVISFHACFLQFYLLFSLGTTEFFFLSAMALDRYLAICHPLHYQTIMSPHSCCILVTACWLLGFLGYITPVVLISKLSFCGPNIIDHFLCDLVPLLSVACPPLGNAPLICQIIVHLLFLSNVIFVILSYGIVIYTLMKSSSKGNCRKGFNTISMHLLVVTLFYSTVGAMYVIPVGESYLKVNKIITLFYAAIIPFLNPLIYCLRNDQVKEALHKLLAEISESIMSTITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGE8</accession>
    <name>A0A6I9XGE8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Microtubule-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106543299</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914749.1">
        <property type="nucleotide sequence ID" value="XM_014059274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGE8"/>
    <dbReference type="GeneID" id="106543299"/>
    <dbReference type="KEGG" id="tsr:106543299"/>
    <dbReference type="OrthoDB" id="9378527at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027324">
        <property type="entry name" value="MAP2/MAP4/Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001084">
        <property type="entry name" value="MAP_tubulin-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11501">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11501:SF15">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00418">
        <property type="entry name" value="Tubulin-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00229">
        <property type="entry name" value="TAU_MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51491">
        <property type="entry name" value="TAU_MAP_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013914749.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="20250" checksum="2625C292F9AD32DB" modified="2020-10-07" version="1">KSIFLVTIRTPPKSPATPKQLRLINQPLPDLKNVRSKVGSTDNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVRLDFKEKAHAKVGSLDNAHHVPGGGNIKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGS4</accession>
    <name>A0A6I9XGS4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carcinoembryonic antigen-related cell adhesion molecule 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106543415</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914899.1">
        <property type="nucleotide sequence ID" value="XM_014059424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGS4"/>
    <dbReference type="GeneID" id="106543415"/>
    <dbReference type="KEGG" id="tsr:106543415"/>
    <dbReference type="OrthoDB" id="6159398at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052598">
        <property type="entry name" value="IgSF_CEA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337:SF20">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="21"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="117"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013914899.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22358" checksum="3F4558B9145B1CCF" modified="2020-10-07" version="1">HDFFPPTYDFPVFDPDGPEHPVISPLQQVYNEHSTLNLYCSAVCNPPAKFSWYLNGELLARQNESRLAQRLTLKNAGNYTCRVDNVETGLSKAVGLEISIREAINHISVRDPGKIVENSTTYLNCTASGFNVSYYWLKGKQRLQAGARLSLSSNGQNLSLSKTSRQDSGLYTCWGINSFSNNSFNYTLNVFCKSGSALGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI18</accession>
    <name>A0A6I9XI18_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">GPI ethanolamine phosphate transferase 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PIGG</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGG subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913552.1">
        <property type="nucleotide sequence ID" value="XM_014058077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI18"/>
    <dbReference type="GeneID" id="106542370"/>
    <dbReference type="KEGG" id="tsr:106542370"/>
    <dbReference type="CTD" id="54872"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051267">
        <property type="term" value="F:CP2 mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16024">
        <property type="entry name" value="GPI_EPT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000018">
        <property type="entry name" value="Putative GPI ethanolamine phosphate transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037674">
        <property type="entry name" value="PIG-G_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039527">
        <property type="entry name" value="PIGG/GPI7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045687">
        <property type="entry name" value="PIGG/GPI7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19316">
        <property type="entry name" value="PIGO_PIGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="466"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="502"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="698"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="720"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="762"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="799"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="836"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="884"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 2 C-terminal" evidence="12">
        <location>
            <begin position="651"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913552.1"/>
        </source>
    </evidence>
    <sequence length="913" mass="102336" checksum="1FD363631E38484D" modified="2020-10-07" version="1">MRLRSGVFASFCLLVQALGVGLFIRGFFPAPVRSLLPSGTFGQVPAEPPQTELTSDWTEKIPPIFNKVVIVLIDALRDDFVFGSKGLKFMPYTTELVEKGTTHAFIAEAKAPTVTMPRIKALMTGSIPGFIDVITNLNSPALMEDNLIWQAKRAGKRIIFYGDDTWIRLFPKHFVEYDGTTSFFVSDYTEVDNNVTRHLNNVLKREDWDMLILHYLGLDHIGHLSGPNSPLIGPKLSEMDNIISQIHKSLLSEELEKGTLPNLLVVCGDHGMSETGNHGGSSESEIRTPLLFVSSAFQRTGGPRKYQELIQQTDLAVTLALGLGLPISRNSVGNLLSPVIEWKTPREQLRLLHLNGFQLSNLLKENVPTYEKDPGFEMFVKAEKAHGNWIQYYLDGSNTQVVTNLSKKIIKQYLEALKILSFSLSKQVAQYDMYSMIIGTMIIVEVLFLLLLSLPKALSSRAEFEVPNSSLCSLMFYLICLLWSAIHVVVCTSSESLCYFCSIPWILAAGIIVLISGLFCIIISLLEMRVINSRQLLKNPAAAPSSWSELDVLILAGTIGHISSLGASSFVEEEHQTWYFLINTLCLALCQEICRHYFLTKEGDLQIPTTGKAKAEEMKETPSHYRSDIEITEDELKMGRGDNPVSVNTFEKWMALATPWLILIICRVLRSLNQTGVQWAHRPDFGHWLTSSEHKTELSLLASVSLVMILILVHRRCSLVSKIAMALGLLGVYSYQAAIGNVEFPWQQDRKDISKGTIEARFVYIFVLGIIFTGTKDLFKSLFFSMDTKIKFKGLWEIYGGLVLLSALLLRPHNLPVLVLCLFIQTMMRNYIWKPLEFDAAQVTIMHYWFGQAFFFFQGNSNGIASVDVSAGFVGLTDYVEIPAIFLTALATYSGPLLWAIHLLCYLSSEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJN0</accession>
    <name>A0A6I9XJN0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hepatic triacylglycerol lipase</fullName>
            <ecNumber evidence="6">3.1.1.3</ecNumber>
            <ecNumber evidence="5">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Lipase member C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Lysophospholipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Phospholipase A1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">LIPC</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of triglycerides and phospholipids present in circulating plasma lipoproteins, including chylomicrons, intermediate density lipoproteins (IDL), low density lipoproteins (LDL) of large size and high density lipoproteins (HDL), releasing free fatty acids (FFA) and smaller lipoprotein particles. Also exhibits lysophospholipase activity. Can hydrolyze both neutral lipid and phospholipid substrates but shows a greater binding affinity for neutral lipid substrates than phospholipid substrates. In native LDL, preferentially hydrolyzes the phosphatidylcholine species containing polyunsaturated fatty acids at sn-2 position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = 2,3-di-(9Z)-octadecenoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38391"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:38392"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1,2,3-tributanoylglycerol + H2O = dibutanoylglycerol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40475"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:35020"/>
            <dbReference type="ChEBI" id="CHEBI:76478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:40476"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38699"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
            <dbReference type="ChEBI" id="CHEBI:76083"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:38700"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38511"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38512"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41384"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64563"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:41385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + H2O = 3-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38651"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
            <dbReference type="ChEBI" id="CHEBI:75938"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:38652"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 30">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="6"/>
    <dbReference type="EC" id="3.1.1.5" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013914231.1">
        <property type="nucleotide sequence ID" value="XM_014058756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJN0"/>
    <dbReference type="GeneID" id="106542898"/>
    <dbReference type="KEGG" id="tsr:106542898"/>
    <dbReference type="CTD" id="3990"/>
    <dbReference type="OrthoDB" id="199913at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034185">
        <property type="term" value="F:apolipoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01758">
        <property type="entry name" value="PLAT_LPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000010">
        <property type="entry name" value="hepatic triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000441">
        <property type="entry name" value="Lipoprotein lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002333">
        <property type="entry name" value="Lipase_hep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF2">
        <property type="entry name" value="HEPATIC TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00824">
        <property type="entry name" value="HEPLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0345">HDL</keyword>
    <keyword evidence="9" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PLAT" evidence="32">
        <location>
            <begin position="415"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_013914231.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="63134" checksum="638D0B082BFE4CB5" modified="2020-10-07" version="1">MMTLVSGSWVLSSSASFQIQRSSVLSTDHPWMIITSGREALKGHCLSASSWPGSIRQPCSALSPSCPRASLQQEGRREGAAAEGPGGSSWRLCPQTPGQAGSQGWRGSSRFEAKFLLYSNRAEQGCLIEPGQEEALKQCPFNASLPLVMIIHGWSVDRTLERWIWKLAEELKTQLPDTNVIITDWLSLAHAHYPIAVQNTRDVGQEIARFLEWLEETVRFHRSKAHLVGYSLGAHVAGFAGSSMRGNGKIGRITGLDPAGPLFEGMSPTDRLSPDDADFVDAIHTFTQQHMGLSVGIKQPVAHFDFYPNGGTFQPGCHIMHVYSHIVQYGITGLSQTVKCAHERSVHLFIDSLRYSQKQITGYWCKNMEMFDKGRCLDCRAHRCNTLGYHIRKARIPGSQRLFLKTQPQMPFKVYHYQFKIHFIHEFQEPRIDPTFTISLTGNKDDVENLSITLIEGITQNKTYTFLVPLDIDIGELMMIKLKWEGTAIWENIWDTFQTIIPWRKGDRRPGLIVTSIRVKAGETQQKMTFCSQRVDNLHLYPGHEKMFVRCEVSSQKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK24</accession>
    <name>A0A6I9XK24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PPP2R2B</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914361.1">
        <property type="nucleotide sequence ID" value="XM_014058886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK24"/>
    <dbReference type="GeneID" id="106543000"/>
    <dbReference type="KEGG" id="tsr:106543000"/>
    <dbReference type="CTD" id="5521"/>
    <dbReference type="OrthoDB" id="6274823at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013914361.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="53021" checksum="9A8CCAD5E6383884" modified="2020-10-07" version="1">MRLCRWKMKCFSRYLPYLFRPPNTILSSSCHTEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQPHRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEDGRLRDPSTITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRTSALCDRHSKFFEEPEDPSNRSFFSEIISSISDVKFSHTGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAATNNLYIFQDKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK52</accession>
    <name>A0A6I9XK52_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methanethiol oxidase</fullName>
            <shortName evidence="3">MTO</shortName>
            <ecNumber evidence="3">1.8.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Selenium-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106540615</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria. Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>methanethiol + O2 + H2O = hydrogen sulfide + formaldehyde + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="EC" id="1.8.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Organosulfur degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">May associate with Golgi membrane. May associate with the membrane of autophagosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the selenium-binding protein family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.4" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013911273.1">
        <property type="nucleotide sequence ID" value="XM_014055798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK52"/>
    <dbReference type="GeneID" id="106540615"/>
    <dbReference type="KEGG" id="tsr:106540615"/>
    <dbReference type="OrthoDB" id="10252446at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018549">
        <property type="term" value="F:methanethiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300:SF0">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0711">Selenium</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013911273.1"/>
        </source>
    </evidence>
    <sequence length="84" mass="9426" checksum="4DE9FD4FA51338FC" modified="2020-10-07" version="1">MIQLSLDGKRLYVSTSLYSGWDKQFYPDMVKEGSVMLQIDVDSKKGGLKVNEKFLVDFGKEPNGPALAHEIRYPGGDTTSDIWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK62</accession>
    <name>A0A6I9XK62_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ral GTPase-activating protein subunit alpha-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RALGAPA2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914317.1">
        <property type="nucleotide sequence ID" value="XM_014058842.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542962"/>
    <dbReference type="KEGG" id="tsr:106542962"/>
    <dbReference type="CTD" id="57186"/>
    <dbReference type="OrthoDB" id="19311at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000001">
        <property type="entry name" value="Ral GTPase-activating protein subunit alpha-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046859">
        <property type="entry name" value="RGPA/RALGAPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF2">
        <property type="entry name" value="RAL GTPASE-ACTIVATING PROTEIN SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20412">
        <property type="entry name" value="RALGAPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="4">
        <location>
            <begin position="1568"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="661"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="727"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="661"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="734"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013914317.1"/>
        </source>
    </evidence>
    <sequence length="1806" mass="205121" checksum="AA546899C06FA64F" modified="2020-10-07" version="1">MFSRRGHADVKKSAQKALDPRKEPLTRLKHLRALMDGVDGSELKQFFEINYSQIYLIFYESFVTLETSLKQKGNKSQREELDSVLFLFERILQLLPERIFYRWHFHSIGSILKKLLHTGNCLKIRCEGIRLFLLWLQALQTNCAEEQLLIFACLVPGFPAVLSSKGPCTLDTLISPPSSLPNGKIYPEDITPLLPHATGEKSEDPTCYFLQILLKYMVIQAAKVEWKNKENLNTGFKFLFALFRKYYLPHLFPSFIKLTNLYNPVLGIPGMRPKSVYVSTTCDGGNVYNTKIPYLDARVIFIKWLVNFFLEKKYPTAAQTFKNGVEVLPRTIQTVGNTAQDKNPEVENSVPSDQEKNHINHSGILSDSSFCSIEEQHRTAYEIVQQIFLSTRDYVNFVNEVFHQAFLLPPSEISLTEKIVKVYRKWILHEKPLFMEEPDAKETTQDDDVITEHSTEETDGKQLVSRHRRSSSWGRSYSFSSAVTRGCLLEDENENVKAGLQATLQVFLTNSANIFLLEPCSEAPELLKEQINSCRAVLSIYRRMIMEVPMNKKTWEHILQILLSITEAVMSNSKDDQIKDAFGQSLAASLFRTLIVAWIRANLSVYISRELWDELLRVLSSLTDWEELIMEWANIMDSLTSVLARTVYGVEMTNLPLDKLSEQKEKIQKGRGKSEQQELSENGSGIVNQQPVNLLGAEYKSPFSDLDQLTRSSSTSDIRDQCSSDVFQVRKTESSQNLTSGSRSVQKNETTNKENTLQEESNTPSELNLKTDIQHGNQKEKEKCESISSDTSIGYNHEMGMSLATWQTCEENNDISMHADISVDPEAHQWLATNFSDSSEFLADDYSIIAGGTLTGWHPDSAAVLWRRTLGILGDVNNIQSPKIHARVVEYLFELWHKLAKIRDNLAISLDNQSTPCPPVLIPPLRIFASWLFKATTLPDEYKEGKIHAYKLICAMMTRRQDFMPNPDYLVHFYLIMHIGLTNKDQDVLNTIIKHCSPFFFFLRLPGFTLLIRDFITATTRILSTNMLEAPRLEANTILGSLICFPNLYQDILMLSSVTEAGITTGTADVKLCLINILLKNATEEPSEASRYLALCCLGLWICEELVNCTNHPQVKDAINVFGVTLKFSNKQIAQAACDIFQLLTSYWEKLQKYNISLPQKIIEVLVATIAFLLPSAEYSSVESDKKFIVSLLLCLLDWCMTLPVTILLEPVFTSNFENQYSSNTPLLDYIYRILNCCVYGSSLYNQQSHYLLSLADLSSEIYDPFMPLGNVRNSEVILVQSSADLNNLLTVAEEMKRRSLELIPLTARMIMTHLVNNLGHYPLTEGPAVLHSLISENHSNSCMESSELSPEIFRSPNLQIFEFNDNAVISYLQIPSKRGMADELEDSFSEVRVIVRDISGKYSWDGQLMHGPLGACSLDQMNRKFSVVMEKNKQNFESPTDFIQIDDGEDALDRLLEKIGSSSPECLLHPQIKLNEPSPSPSVMSHAQANDIIEAIMQQSCIENEYAHGYHLNHNTKSENQKMPRSALPQSPFHLCRLLLNDLGMNSWEKRKSFNILKKNSKLLRELKNLDSRQCRETHKIAVVYIAEGQEDKCSILSNPQGSQAYEDFIAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHVIWSEHTRNYRRGIIPTDFGDVLIVIYPMRNRMFYIEIMKKPQVPFFGPLFNGAIVTGKLLPNLIRATCVNASRAVKSLITLYQSFYEERAIYLDEIIRNHKELLSFEDFAAQVFSPSPSYSHSGCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK97</accession>
    <name>A0A6I9XK97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DEAD box protein 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DDX6</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013911318.1">
        <property type="nucleotide sequence ID" value="XM_014055843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK97"/>
    <dbReference type="GeneID" id="106540651"/>
    <dbReference type="KEGG" id="tsr:106540651"/>
    <dbReference type="CTD" id="1656"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000364">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="127"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="308"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013911318.1"/>
        </source>
    </evidence>
    <sequence length="483" mass="54344" checksum="8BE52CD0593BB5FD" modified="2020-10-07" version="1">MSTARTENPVIMGLSSQNGQLRGPVKPGGGPGSGGTQAQQQINQLKHPNTINNGTQQQAQSMASTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDKIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHIQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLP7</accession>
    <name>A0A6I9XLP7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Interferon regulatory factor 2-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">IRF2BP2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IRF2BP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914847.1">
        <property type="nucleotide sequence ID" value="XM_014059372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLP7"/>
    <dbReference type="GeneID" id="106543366"/>
    <dbReference type="KEGG" id="tsr:106543366"/>
    <dbReference type="CTD" id="359948"/>
    <dbReference type="OrthoDB" id="10065080at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16716">
        <property type="entry name" value="vRING-HC_IRF2BP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1580:FF:000001">
        <property type="entry name" value="interferon regulatory factor 2-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1580">
        <property type="entry name" value="Interferon regulatory factor 2-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044882">
        <property type="entry name" value="I2BP1/2_C3HC4-RING_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058682">
        <property type="entry name" value="IRF-2BP1/2-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022750">
        <property type="entry name" value="IRF-2BP1_2-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057414">
        <property type="entry name" value="Zf-C3HC4_IRF-2BP1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF18">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 2-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11261">
        <property type="entry name" value="IRF-2BP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25457">
        <property type="entry name" value="IRF-2BP1_2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25454">
        <property type="entry name" value="zf-C3HC4_IRF-2BP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Interferon regulatory factor 2-binding protein 1/2-like zinc finger" evidence="11">
        <location>
            <begin position="13"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="IRF-2BP1/2-like middle" evidence="13">
        <location>
            <begin position="289"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon regulatory factor 2-binding protein 1/2-like C3HC4 zinc finger" evidence="12">
        <location>
            <begin position="494"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="63"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="209"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="310"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="428"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013914847.1"/>
        </source>
    </evidence>
    <sequence length="577" mass="59995" checksum="064C3BDA226E1D55" modified="2020-10-07" version="1">MSSAAVAVAAASRRQSCYLCDLPRMPWAMIWDFTEPVCRGCVNYEGADRVEFVIDTARQLKRAHSFQEGRAPPPPHTKQQPPPLSAKDLHSAAVATAEAASRAPPQPHERYSLAAALASSERPPPRLGPEYGGSRQVNGILVPNGFSKPEEPPELNRQSPNPRRTHAVAPTLVPLMNGASLPASISISSRAAAAAAASLAAISGAPPTPLQVSVSQAQQSQQQPGEGGGHKRPGSVSSSSSGGGGGGGGGASDQEGKEKGQPQAARGSADPLDPLGVESKARGAAAGSEQEWLGKPKTVRDTLLALQHSGHAAPFESKFKKEPGMAGGRLLGYDSNGAAAKSGARAARKRKTSPEPEGEVGPPKINGEAQSWLPTSSEGMKIPMAAASFMSPPPPTASPHSNRTTPPEAVQNGQSPMAALILVADNAGGNHASKDANQVHSTTRRNSSSPPSPSPMNQRRLAREVPSQGANPGGMEPVHPVSLPDSSLAASVPLCCTLCHERLEDTHFVQCPSVPSHKFCFPCSRQSIKQQGANGEVYCPSGEKCPLVGSNVPWAFMQGEIATILAGDVKVKKERDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLY8</accession>
    <name>A0A6I9XLY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HAO1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013911848.1">
        <property type="nucleotide sequence ID" value="XM_014056373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLY8"/>
    <dbReference type="GeneID" id="106541047"/>
    <dbReference type="KEGG" id="tsr:106541047"/>
    <dbReference type="CTD" id="54363"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF107">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 10" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="291"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="314"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013911848.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="40805" checksum="8528D5FB9C852C60" modified="2020-10-07" version="1">MSERPICIADFEEYAKKFLQKSVYDYYKSGADDQQTLAENVAAFSRLRLYPRMLKNVSTLDLTTCVLGEKISMPICIAATAMQCMAHADGEIASARACRSAGTGMMLSSWSTTSIEEVAQAAPKTIIWLQLYIYKDRDVTRSLVHRAEKAGYKGIFVTVDTPYLGKRLDDVRNKFRLSPHLRMKNFETNDLAFSSEKGYGENSGLSVYVTEAIDPSINWDDLKWLRGLTALPIVAKGILRADDAREAVKIGVNGILVSNHGARQLDGVPATIEVLPEIIDAVEGKTEVFLDGGIRKGTDVLKALALGAKAVFVGRPVIWGLAYQGEDGVKEVLQILKEEFKLAMALSGCPSVEAIDRTLVRRVQWEASKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XN39</accession>
    <name>A0A6I9XN39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106543780</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915336.1">
        <property type="nucleotide sequence ID" value="XM_014059861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XN39"/>
    <dbReference type="GeneID" id="106543780"/>
    <dbReference type="KEGG" id="tsr:106543780"/>
    <dbReference type="OrthoDB" id="9889152at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0552">Olfaction</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013915336.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="35457" checksum="EDFAC6A9E9211EB8" modified="2020-10-07" version="1">MGNSSQVTEFILTGLTDDPQLQLILFVIFFTLYAITLLGNVSMIVLFRISPKLHTPMYFFLSHISFVDICTASIITPKFLSHLVKEKKVISFAGCFIQTYFYGVFSTAECYILAIMAYDRYVAICHPLLYLVIMSQLKCVRLVVVSYVTGVLHGLLHIIPATQLFFCGPNIIKNFVCDGVSLLQLACSDISFNNLLKFIFIGFTLIATMVIVLTSYIYILIAIFKMDSAKSQKKAFSTCTSHLMVITIFYGCAGFVYAQSQPTKSIYINQIASVLYLVVTPMINPFIYSLRNQEVKVAFRKILGGKNCVIFYTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP22</accession>
    <name>A0A6I9XP22_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">THUMP domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">THUMPD1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915616.1">
        <property type="nucleotide sequence ID" value="XM_014060141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP22"/>
    <dbReference type="GeneID" id="106544005"/>
    <dbReference type="KEGG" id="tsr:106544005"/>
    <dbReference type="CTD" id="55623"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915616.1"/>
        </source>
    </evidence>
    <sequence length="155" mass="16782" checksum="F1320C4D47F35268" modified="2020-10-07" version="1">MAAAAAPIAAAASPMEASVETPPVAKAAPVRRKRKPPKGKYAHLSKRPKVLGPRKLEAGMSGILITSNVGQQKCVAEAYNLLSEYGEQLYGPGEFIEQKVVRLGEEEEDDAEAALKKEVDQIRTSTEQKRRRFQSLPSGANNVFFIRTQGVGMCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPH2</accession>
    <name>A0A6I9XPH2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongin-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TCEB3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014060432.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I9XPH2"/>
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    <dbReference type="KEGG" id="tsr:106544221"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="RNA_pol_II_trans_fac_SIII_A"/>
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        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141:SF75">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF06881">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
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        <location>
            <begin position="562"/>
            <end position="606"/>
        </location>
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        <location>
            <begin position="132"/>
            <end position="174"/>
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            <end position="465"/>
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            <begin position="703"/>
            <end position="748"/>
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        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
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        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
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            <begin position="232"/>
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            <begin position="276"/>
            <end position="294"/>
        </location>
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        <location>
            <begin position="305"/>
            <end position="315"/>
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            <begin position="322"/>
            <end position="335"/>
        </location>
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        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
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        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
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        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
    </feature>
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        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="716"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
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            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013915907.1"/>
        </source>
    </evidence>
    <sequence length="763" mass="86450" checksum="2F8F8D4D60DBC423" modified="2020-10-07" version="1">MAESVLEDVRKLQSRLTSASEPKKLLKSLKRLSELPMTIDILQETGVGKSVNSLRKHEQVGIFAKNLVAIWKKLLPVSQEKVRHNLEPTEHDRNLSRKRHRDLLPKEGCSETHQPSCSQSYELDCGTKKVKRCSEPEKSHQEVSYGIPQDRSWRRGSSDQECSDYGHAVSSELSESPQELYVDLCHAEEQEKEQKSFSQKGMKSPPFQDRPEVTHHRHFSDSQEKPSTSQSKEPKSSHKERQRPEAKGEDKASVFSSERLPKASSSFHEASLPGSSKEKLKAPDSLKREKKKDGASSSGIKRQKTHFDLEDSLNEHRKKQKTHDLEKAKAEKIKFSLETICVDQEKPKPEGDFPHKNKEKKSSSSFKTSEAKPKVFESHKRPAGFSPPGEVEDEYEQPTMSFESYLSYDQPQKKKRKLAKPTTAPAPEKAHSSKQNGSKSSAKSSDASRKAPKPPSEKKPVEASAPKTKKILIDVLPMLPDIPLPPIQANYRPLPSLETVPFVQPKRKALSSPTAEDEAGFTGRRLNSKMQVYSGSKTAYLPKMMTLHEQCIRVLSNNIDSIYEIGGVPYSVLEPVLERCTPEQLYRIEECNHVLTEDTDQLWHNHCQRDFKKEKPDEFESWREMYLRLHDAREQRLLMLTQNIRSAHANKPKARVAKMAFVNSTVKPPREVRRRQEKFGTGGAAAPEKIKIKPVLFTATKSSSVHAEEEQDYDRPSTSSGPSCSSMGSTSSVSYDPRKPQVKKIAPMMAKTIKAFKNRFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPX6</accession>
    <name>A0A6I9XPX6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="17">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">UCKL1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">May contribute to UTP accumulation needed for blast transformation and proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 17">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with RNF19B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013918084.1">
        <property type="nucleotide sequence ID" value="XM_014062609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPX6"/>
    <dbReference type="GeneID" id="106545897"/>
    <dbReference type="KEGG" id="tsr:106545897"/>
    <dbReference type="CTD" id="54963"/>
    <dbReference type="OrthoDB" id="10257085at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000010">
        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000200">
        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="19">
        <location>
            <begin position="84"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="20">
        <location>
            <begin position="314"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013918084.1"/>
        </source>
    </evidence>
    <sequence length="531" mass="59721" checksum="938671630E1502BC" modified="2020-10-07" version="1">MASADGVVDEAVPATRRSSGSKGAALNGLVGRLPLTPSLSPRKRTTSQSKTEPPLLWTSKRTIYTAGRPPWYNETGTPFKEAFVIGLCGGSASGKTTVANKIIEALDVPWVVLLSMDSFYKVLNKEQQEQAACNEYNFDHPDAFDFDLLISVVRKLKEGKSVKVPVYDFTTHSRSKEWKTIYGANVVIFEGILSFANKELLQLLDMKVFVDTDSDIRLVRRLKRDIMERGRDVGGVIKQYSKFVKPAFEQYIEPTMQAADIVVPRGGENFVALDLIVQHVHSQLEKRKLRWHIAALASAHQGQPLPTTLSVLESTPQVRGMHTIIRNKDTSRDEFIFYSKRLMRLLIEYALSFLPLKPVTVETPQGTIYDGKRFHRQRITGISILRAGETMEQALTAVCKDIRLGKILIQTNHDTGEPELHYLRLPKEISEDYVILMDSTVSTGAAAMMAIRVLLDHDVQEDRIFLLSLLMAEMGVHSVAYAFPRVHIITTAVDKRVNEEFHIIPGIGNFGDRYFGTDGTMPWYENDDMDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQT9</accession>
    <name>A0A6I9XQT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Olfactory receptor 14A16-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106544413</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916146.1">
        <property type="nucleotide sequence ID" value="XM_014060671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQT9"/>
    <dbReference type="GeneID" id="106544413"/>
    <dbReference type="KEGG" id="tsr:106544413"/>
    <dbReference type="OrthoDB" id="9031796at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916146.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="21600" checksum="EC8F3D252E5F6901" modified="2020-10-07" version="1">MAYDRYIAICHPLHYEMVMNWKYCAEIIFLVWVTGILYGILHTSGSFSIHFCSNVVNQFFCEIPQLLKLSCSGLKLIEVGVLVFSIIVGLDCFTFIVISYVVIFKTVLRIPSEKGRQKTLSTCIPYLTVVSMLLLSSCIGYLRPSPYTPSYLDIGLTVLYALLPPLFNPIIYSMRNKNIKCVLSQLWRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRG0</accession>
    <name>A0A6I9XRG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cystine/glutamate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Amino acid transport system xc-</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Solute carrier family 7 member 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">xCT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SLC7A11</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-cystine(out) + L-glutamate(in) = L-cystine(in) + L-glutamate(out)</text>
            <dbReference type="Rhea" id="RHEA:70995"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-acetyl-L-cysteine(out) + L-glutamate(in) = N-acetyl-L-cysteine(in) + L-glutamate(out)</text>
            <dbReference type="Rhea" id="RHEA:74567"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:78236"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an L-alpha-amino acid(in) + L-kynurenine(out) = an L-alpha-amino acid(out) + L-kynurenine(in)</text>
            <dbReference type="Rhea" id="RHEA:71191"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc; this interaction mediates cell membrane localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913268.1">
        <property type="nucleotide sequence ID" value="XM_014057793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRG0"/>
    <dbReference type="GeneID" id="106542144"/>
    <dbReference type="KEGG" id="tsr:106542144"/>
    <dbReference type="CTD" id="23657"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015811">
        <property type="term" value="P:L-cystine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015813">
        <property type="term" value="P:L-glutamate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000027">
        <property type="entry name" value="cystine/glutamate transporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004760">
        <property type="entry name" value="L_AA_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00911">
        <property type="entry name" value="2A0308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF323">
        <property type="entry name" value="CYSTINE_GLUTAMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="315"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013913268.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="55213" checksum="DAA4D1ABED33E261" modified="2020-10-07" version="1">MFRKSPVSSCSNGSSPQGQSKLPSTYDIKVEPGDQVVLKKKITLLRGISIIIGTIIGAGIFISPKGILQNTGTVGMSLIVWTVCGILSLFGALCYAELGTSIKKSGGHYTYILEAFGPLPAFVRVWVELLIIRPAATAVISLAFGQYILEPLFIQCEIPDLAVKLITAVGITVVMVLNSMSVSWSARLQIFLTFCKLLAILIIIVPGVMQLIRGKTEHFKDAFVGNAASVKGLPLAFYSGMYAYSGWFYLNFVTEEVENPERNVPLAICSSMVIVTLGYVLTNVAYFTTLSPKDMLISKAVAVTFSERLLGNFSLAVPIFVALSCFGSMNGGIFAVSRMFYVASREGHLPKILSMIHVRKHTPLPAVIVLYPLTMIMLFTSDLYSLLNFLSFARWLFIGLAVAGMIYLRYKCPDMPRPFKVPLFIPALFSFTCLFMVALSLYADPVNTGIGFAITMTGVPAYYLFIIWDKKPTWFRRFLDNMTSTLQIILEVVPTEENPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUI6</accession>
    <name>A0A6I9XUI6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13 14">Heterogeneous nuclear ribonucleoprotein D0 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13 14" type="primary">HNRNPD</name>
    </gene>
    <organism evidence="11 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919747.1">
        <property type="nucleotide sequence ID" value="XM_014064272.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919748.1">
        <property type="nucleotide sequence ID" value="XM_014064273.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919749.1">
        <property type="nucleotide sequence ID" value="XM_014064274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUI6"/>
    <dbReference type="GeneID" id="106547197"/>
    <dbReference type="KEGG" id="tsr:106547197"/>
    <dbReference type="CTD" id="3184"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12583">
        <property type="entry name" value="RRM2_hnRNPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000030">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000156">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF3">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 13 14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="74"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="159"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="248"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013919748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013919749.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="36364" checksum="DEC24658B715ABD4" modified="2020-10-07" version="1">MSDDQFAGDEALAEASEESEQLEGILAPGGDGMEEAEGGGGEGEGGGGGGGGGGGSGESEGAKIDASKNEEDEGKMFIGGLSWDTTKKDLKDYFTKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVEKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKDEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGTRGGFSGRTRGRGGGSSQSWNTGYNNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVI3</accession>
    <name>A0A6I9XVI3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Parathymosin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PTMS</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920154.1">
        <property type="nucleotide sequence ID" value="XM_014064679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVI3"/>
    <dbReference type="GeneID" id="106547479"/>
    <dbReference type="KEGG" id="tsr:106547479"/>
    <dbReference type="CTD" id="5763"/>
    <dbReference type="OrthoDB" id="9050028at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920154.1"/>
        </source>
    </evidence>
    <sequence length="117" mass="13501" checksum="1FE4E39EDC47BB58" modified="2020-10-07" version="1">MSEKSADEAPVELGAKELKEKKEKHEEKSARKDKKEIIEDDENGAEEEEDDEDANPEDVDDEDEEDEDDENEREPDTHAVKRSAEKKQEEKPLGFTFQIAGWRNRCNSSLIFHSPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWI9</accession>
    <name>A0A6I9XWI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CHPF</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus</location>
            <location evidence="1 10">Golgi stack membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013918141.1">
        <property type="nucleotide sequence ID" value="XM_014062666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWI9"/>
    <dbReference type="GeneID" id="106545939"/>
    <dbReference type="KEGG" id="tsr:106545939"/>
    <dbReference type="CTD" id="79586"/>
    <dbReference type="OrthoDB" id="9985088at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050510">
        <property type="term" value="F:N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000004">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF22">
        <property type="entry name" value="CHONDROITIN SULFATE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="42"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013918141.1"/>
        </source>
    </evidence>
    <sequence length="772" mass="87735" checksum="ED8CA13EA5C18404" modified="2020-10-07" version="1">MRFSSLLSLLRPVGPVVVGVSLGFTLSLLSVTWVEEPCGPGHGSFREGGLEQHQHPGGQGNAARKPNSLSGGLGTEPEQSWEPRVLPYKPLNPGKATKKTIRTRYISTELGMRQRLFVGVLTSKNTLNSLAVAVNRTLGHRLERLIYFTGTRGRKVPHGMMVVTHGDERPIWNMYQTVKYVIDHYLSDFDWVFLVQDDTYVEAHRVNHLASHLSIDTLLYMGRPEEFIGGDTQGHYCYGGFGYLLSRALLLRLQPHLESCRNDILSARPDEWLGRCIIDYTGVDCVAEQEGLRYHYFELGKNTDPEKESDPQFNAAFTVHPVLDPLQMYRLHRHFARVELEHTYQEIQQLQLEIQNASSLSADGELSSTWPIGIPPPFQPKTRFEVLHWDYFTEEAAFSCVDGAPKCELQGSDAADVADVVAAAVEELNRRYQPLLHVRKQQLLNGYRRFDPTRGMEYTLDLQLEVVTQKGHSRSLVKRVHLLRPLSEVEIIPMPYVTEASRVNVILPLTASEKDYATHFLDVYAAAAFENSENAVLTFLFIYDPFEAQKVAQNDVFGGVKAHITEYERRHPQVKIPWISVKTDVPSQIKVMDIISKKHPVDTLFFVAGVSTEVTTEFLNRCRMNTINSWQVFFPIHFQGYNPTIAFHNQPPPLSGLDLVRDAGHFDREVFNEACFYNADYMAARTRMAADAQDNEDLLETMDIYDLFVKYSNLHVFRAVEPALLQPFRPQICNPRLSEEQYHRCVQSSLEGLGSRSQLAMVLFEQEQGNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXL5</accession>
    <name>A0A6I9XXL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106543794</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915348.1">
        <property type="nucleotide sequence ID" value="XM_014059873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXL5"/>
    <dbReference type="GeneID" id="106543794"/>
    <dbReference type="KEGG" id="tsr:106543794"/>
    <dbReference type="OrthoDB" id="9823959at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="40"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915348.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="34957" checksum="9302BBF454102A53" modified="2020-10-07" version="1">MQTNQTTVTEFILVGFMDHPELQIPLFVFFLLIYLITLVGNIGIIIVTKIDARLHTPMYFFLRNLSVIDLGLSTTIAPKLLATFMIEHSTISFTGCTTQFFFFVIFVTTEGCILAVMAYDRFIAICNPLLYFVVMSKKLCILLVIAAYTCGFSSSMVHTIVIFNLNFCSSNKINHFFCDVPPMLQLSCSDTHITRIVHFILSTAIALTTFLTVLISYIAIVIAILKISSAQGRYKAFSTCASHLTTVTIFFGTIIFMYVGPGSNFSLNKDKIVSVFYTLIISSLNPLIYSLRNREVKHAFSRILDKITLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXM9</accession>
    <name>A0A6I9XXM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Interleukin-6 receptor subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Interleukin-6 signal transducer</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Membrane glycoprotein 130</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Oncostatin-M receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23 24" type="primary">IL6ST</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23 24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918566.1">
        <property type="nucleotide sequence ID" value="XM_014063091.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918572.1">
        <property type="nucleotide sequence ID" value="XM_014063097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXM9"/>
    <dbReference type="GeneID" id="106546253"/>
    <dbReference type="KEGG" id="tsr:106546253"/>
    <dbReference type="CTD" id="3572"/>
    <dbReference type="OrthoDB" id="9934532at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070102">
        <property type="term" value="P:interleukin-6-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000281">
        <property type="entry name" value="Cytokine receptor like factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000414">
        <property type="entry name" value="Interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000524">
        <property type="entry name" value="Interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000542">
        <property type="entry name" value="Interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003529">
        <property type="entry name" value="Hematopoietin_rcpt_Gp130_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010457">
        <property type="entry name" value="IgC2-like_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06328">
        <property type="entry name" value="Lep_receptor_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01353">
        <property type="entry name" value="HEMATOPO_REC_L_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 23 24" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636620" description="Interleukin-6 receptor subunit beta" evidence="20">
        <location>
            <begin position="27"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="622"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="131"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="229"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="426"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="520"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="720"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="832"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="751"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013918566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013918572.1"/>
        </source>
    </evidence>
    <sequence length="905" mass="100906" checksum="3C2B038CD2C0C443" modified="2020-10-07" version="1">MFSKWGMSMQGFYVLLSILQIGVSDGFLNICGYIVPESPVVLSIGSNFTAFCILLDDCHYGKIGAEQVIWKTKTTVVPKEQYSIINKTISSVTFSDTSTLVSPLTCNAELPGGLVQNIYGIQIQLGYPPEKPTNLSCIVSQTQAGSFIQRCSWEPGRDTLLSTNYTLKHRWPTSVHPDCIPQNSNNSCTIPDVVFFVTLEVWVEAKNELGAAESDHIEFDPVTCVKPLPPHKLSLNSGKFPKILMLSWENSLNEPHELKYKIQYKILKSNDWNEIPPEDTASTRTSFTLQELRPNTEYQIRLCCAIHSSKYWSEWNEVIGFTAEDKPSKGPALWRNIETSDSRNWTLGLLWKELSPSEANGVILKYEVSISGRPPLSFPTRLYTLNTTKLILNVENRTYDVSITAFNKIGKSPTSVLLIPATNSKAPVKNIVAFPEDGKLWVNWTPPKESVSKYIIEWQEACSNLSCPTEWQQEPGSKRRTFLKGTIKPYKCYIITVHPLYIGGQGAGESTQAYLKQDVPATGPSVRTKKLGKFEAVLEWEPLSVEEQNGFIKNYTISYRTATGDERVVAVDPSKTEYLLSSLLSNTLYIVHMTAYTESGGTAGSQFTFSTNRFEDGEIEAIVVPVCIAFLLVTLVGVLVCFSKRDMIKKHIWPNVPDPSKSVIAQWSPQTPAKHFNSKEEMYPEGSFTDVSVVEIEAEDKKSLSDQDLKPFDLLRKEKNASEGHSSGIGGSSCMSSPRQSVSDSDEGEPAQNTSSTVQYSTVIPSGYRDQIPSVQVFARSESTQPLLDLEERPEDQQLLVNDSAMPKHRYFKQNGSWDDTATDFEKVEQISPVSEEDTTRLPASQMGESSPDTDKQGMDFVGAFSPNLMEQAGQLENNTDMVTNEEVPKSYLPQTVQQGGYMPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXW8</accession>
    <name>A0A6I9XXW8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 151B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106548260</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM151 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921079.1">
        <property type="nucleotide sequence ID" value="XM_014065604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXW8"/>
    <dbReference type="GeneID" id="106548260"/>
    <dbReference type="KEGG" id="tsr:106548260"/>
    <dbReference type="OrthoDB" id="190434at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026767">
        <property type="entry name" value="Tmem151"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31893">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 151 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31893:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 151B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14857">
        <property type="entry name" value="TMEM151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013921079.1"/>
        </source>
    </evidence>
    <sequence length="504" mass="58049" checksum="FCC37C98C7F55CA4" modified="2020-10-07" version="1">MSSEGEPEPSAGNSPEAAVAATAATTDATEEQRPIKQSLSSSLCRESHWKCLLLSILIYSCLGAIAWCQLTQVTKLSFDSSFKGKSMIYHDSPCSDGYVYIPLAFLVILYVVYLVECWHCRTKSELQYKADVESVYDRIARMKQATPCIWWKAISYHFVRRTRQVTRYCNGDSYTTTQVYHERVNTHVAEAEFDYSHCGFKDISKELMGLEWFSTTRLKFTKCFSFANMESENSYLAQRAHFFTEIEGLDDYMEIREGMKLKNIDFKEVMMAYGDPEHLPWYVSHYAFWVAALMMISWPLRVFMEYQTAHVHYHVEKLVGLEYSVPSVAEEPLYRYRIPRVSTQDSTELEWHICSNRQLIPSYSEAMLLNLTHSSVGNSYSIRRYSEITHGCETCRHASSTSSIFSRHNGSANSQLSLNTSRFSLCQLHGSHRTGLWRSHSSSITDRGCQDDQCCSHSSQLVINENPPTYQEARFFPVLIVHRSEGQERRHFCLRRASCLETSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYS0</accession>
    <name>A0A6I9XYS0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SYMPK</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013918991.1">
        <property type="nucleotide sequence ID" value="XM_014063516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYS0"/>
    <dbReference type="GeneID" id="106546608"/>
    <dbReference type="KEGG" id="tsr:106546608"/>
    <dbReference type="CTD" id="8189"/>
    <dbReference type="OrthoDB" id="331600at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021850">
        <property type="entry name" value="Symplekin/Pta1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022075">
        <property type="entry name" value="Symplekin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245:SF20">
        <property type="entry name" value="SYMPLEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245">
        <property type="entry name" value="SYMPLEKIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12295">
        <property type="entry name" value="Symplekin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="829"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013918991.1"/>
        </source>
    </evidence>
    <sequence length="897" mass="102332" checksum="9334DDE01DD7B3B4" modified="2020-10-07" version="1">MKDMLNHFAKGKIFTKLDLREAYYRIRIRKGDEWKTTFNTPLGSFQYKVMPFGLQGAPTVFMQYINEVLHNHLYCGVLIYLDDILIFSETLEEHVKLVQKVLKKVLAAKLYAKLSKCEFHKTSLDYLGYRISNKGVEMDPGKSALADLLPCLFQSMNIRTLMRIMFQDGFDLPMEPLTLPLSLAGVEQIKQAKEAVKGEKIAKPEGVLIKRRLSTLNLGQAISVVGAASPASPSTEILQAKRRPEPIIPSTQPRLVGASGRKKIFRLSDVIKPLTDTQKEKLKLAAVKRILRSEKAVAYSGAAHARVKILSSLVTQFEVPLKQEILAFVLDDIRNRLDLAFAWLYQEYNAYLSQYPTGSLSNYDECLIGLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVIRKYCEDESRTYLGMSTLRDLIFKRPSRQFEYLHVLLDLSSHEKDKVRQQALLFIKRMYEKDHLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETIKHCLYLYLALLPQNHKLIHELASVYTEAIADIKRTILRVIEQPIRGMGMNSQELLKLVKNCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYNKRLPDVRFLIPVLNGLEKKEVIQALPKLIKLNPIVVKEVFNRLLGTQHGEGNSTVSPLNPGELLIALHNIDSTKCDMKSIIKATNLCFAEHNVYTSEVLAVVMQQLMEQNPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLILKQVWKYPKVWEGFIKCCQRTKPQSFQVILQLPPQQLSAVFEKCPELREPLLAHVRSFTPHQQAHVPNSTMAILEATIKQETEGKELQPLEEGRGPAPPRTKTLRREREEEAEPQPLLPRVSQDMIALRLAQEKALKRQLEEEQKLKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ65</accession>
    <name>A0A6I9XZ65_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein NPAT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NPAT</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915878.1">
        <property type="nucleotide sequence ID" value="XM_014060403.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tsr:106544199"/>
    <dbReference type="CTD" id="4863"/>
    <dbReference type="OrthoDB" id="6287635at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031442">
        <property type="entry name" value="NPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052850">
        <property type="entry name" value="NPAT_LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15087">
        <property type="entry name" value="PROTEIN NPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15087:SF14">
        <property type="entry name" value="PROTEIN NPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15712">
        <property type="entry name" value="NPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein NPAT C-terminal" evidence="3">
        <location>
            <begin position="748"/>
            <end position="1392"/>
        </location>
    </feature>
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        <location>
            <begin position="201"/>
            <end position="243"/>
        </location>
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            <begin position="428"/>
            <end position="472"/>
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            <end position="1086"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1167"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1222"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1313"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1122"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="231"/>
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            <begin position="428"/>
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            <begin position="445"/>
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    </feature>
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    </feature>
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            <begin position="704"/>
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    </feature>
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        <location>
            <begin position="762"/>
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    </feature>
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            <begin position="1064"/>
            <end position="1073"/>
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            <begin position="1169"/>
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            <begin position="1242"/>
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            <begin position="1316"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1335"/>
            <end position="1354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF15712"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013915878.1"/>
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    </evidence>
    <sequence length="1392" mass="151557" checksum="265C985B99F16EE3" modified="2020-10-07" version="1">MLLPSDVARLVLGYLQQEKLLVTCRAFILESSDLKEYAEHCTGEGFVPACLLSLFGKNLITILNEYITMKSKETTNDVPAMMSSLWKKLDYTLSQIRSMQNSVGFSVNQRSRTRSAIADMKKQKCLQQSASSNLGSSPAVFQPGQLTSTTMTATQVVLKPASSVVQTTLNPSAHQAQIQERQINTGDSLTLVSALQERKLNSSVLSPGKRKSDSQKKRSNASGPHLSNCSLRESPLVEKESSPMEEQMIIENAREKILSSKCLQEKLAENINKFLGSDGNMSHTAKQVESGPTIQESSIDELLGLQQGEIHMTEEAIQEILEQTESDPAFQGLFDIFDFGKTKNCRNVSQGIPTQNGTDNSMLVDEENLKALESYIGTKELDDNSHESLSYTDNHSKEACVLKTSCSEHDLTLEQHIKIQTIVSGEFSSGSEMRNQSESVEMEIPDQRTKPKPTFESEQNQFPGTEHNLDSYSKQGTATRNYRIEKDVLCQSVQNLPDLPYNQLDTSQTALSDRTESCSLPNHMLCEKTDAEVLASLKSDESETVNKLSVNCSHSKEIAKNLQQVTDQFVFPVSAQHNRNSPSSSDCQGSNKLESEPCATLVVEASSPNVVELQLEVVGTSNSLNLDAPAADPEPCLKYGKHKLEDTEITQVEMQEPSSSTKADAESLLLTSDNERPTQTTVEANPTTSSECHSPRPDPADETSPGNKVTGGVSSSNQGTGAEPSNIVSLNIIISDDPFISSDTELNNAVSSITGENVPTIILSSPAKSPSKMTRTSKSVTAEESERPGDSALIEQNLLVLRSQDSVVSSLNIQNEDCTVFSVAGTSNVSKDGEFIQLMPATSTSFGSSNSVYIASCISEPTALSTNVTPSNLVMFPGNSTSLASQVPMPPQLRTPPRTNGLFAMNPPMSPNFSQGSAIIIASPIQPVLQGMVGMIPVSIMGQNGNALSDPSRQILRMPVHTSLSSGGIPKLPLPPKSHRMPRSKSNGGGKPVFSGSEPVSRVQRIGNSDKNICLDLKKKPETVAADTTSPGSKQNGSHRRVLCFDTTQASQKEKNENSKFCQSSSNTLLSSTKTQPSKRERERTLPRILCKPDITSKNLAVKESQLEKKCPGPGLASDILRKQTANKENELEKNLVSQTQEVASLSNSQQSLHHEKNCSLAQEVTKKQGLQPNANNKNTVVLPSKEFKKEQTKSPSQVNSLTGPLTKQAVEMLYDIQRHSPASKLPENGELPLPRTPSGTSDRHSDDTFDIRTPTCKRYNEDSATPRIMVPPATPDLPACSPASETGSENSVSMAAHTLMILSRAAIARTSTTTPLKDNSQQFKSIRSASKKRKQEDSVDCEKNSRTTSKKDVQNFALPIKKKKIKQKKLPIAFPAGMDVDKFLLSLHYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZT7</accession>
    <name>A0A6I9XZT7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger E-box-binding homeobox 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Transcription factor 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZEB1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916138.1">
        <property type="nucleotide sequence ID" value="XM_014060663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZT7"/>
    <dbReference type="GeneID" id="106544406"/>
    <dbReference type="KEGG" id="tsr:106544406"/>
    <dbReference type="CTD" id="6935"/>
    <dbReference type="OrthoDB" id="7491548at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000013">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000082">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000122">
        <property type="entry name" value="Zinc finger E-box binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051574">
        <property type="entry name" value="ZnF_E-box_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391">
        <property type="entry name" value="HISTONE H4 TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391:SF17">
        <property type="entry name" value="ZINC FINGER E-BOX-BINDING HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="886"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="914"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="942"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="83"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="479"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="625"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="835"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="990"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
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        <location>
            <begin position="490"/>
            <end position="525"/>
        </location>
    </feature>
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        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
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        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
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        <location>
            <begin position="634"/>
            <end position="648"/>
        </location>
    </feature>
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        <location>
            <begin position="651"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="835"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="856"/>
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    </feature>
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        <location>
            <begin position="1013"/>
            <end position="1027"/>
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    </feature>
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        <location>
            <begin position="1028"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1040"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013916138.1"/>
        </source>
    </evidence>
    <sequence length="1095" mass="121466" checksum="A98F4A0F05D6B38B" modified="2020-10-07" version="1">MTSSSLFIEYQKNHEYRDKIFTPNCVPNSRVSKRLSTKVSVYGGTLLIDCSIKWITNYNNVVETNSDSDDEDKLHIVEEESVVDGTDCDNSVPDDDLPKDHAVLPEHNEKEGSTNSCWEDEGKKETKEILGPEAQIDESGCPVKDECDSDAENEQNHDPNVEEFLQQEDTAVIYPEAPEEEQHQGTPEASGQDENECLEQDKNYVELKSDNPKMRHVTQSSGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGMVPVNGRARSGIKTSQCSSPSLSASPGSPTRPQVRQKIENKPLQEQLPVNQIKTEPVDYEFKPIVVASGINCSAPLQNGVFSGGSQLQATSSTQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENSHTNLTSKEQETINTAPLQQAGHSLISAISLPLVDQDGTTKIIINYSLEQPSPLQVIPQNLKKETSAPNNNCKTEKLPEDLRVKSEKEKSFERETNDSTCLFHDDCPEKPNALQESKHYDVKNSTQPSQLSGAEVEKSESSALSEAGEANPSPGQPPLKNLLSLLKAYYALNAQPSSDELSKIADSVNLPLDVVKKWFEKMQAGQISLPVSGQSSPEPDKLKTSVNNDDHIGSTAENEQDSQSNAVSPLKTNKPQTISTGSAHNGSQHNTPSPSPLNLSSSRNAQGFLYLNESAQEEPQIEPLDLSLPKQHGESLERSTSVYQNSAYSVQEEPLNLTCAKKEPQKDSSITDSEPVVNVIPPSANPINITIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVNPTVQETPKQMQANGNQDERQDTSSEGISNVDDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTELEGAAQEHLGNENAEAGASPSQLDFDERESLTREEEDSEKEEEDEDKEMEEKEKECGKLQEEEEEEEEEEEEEEEEEEEITEESMKDKEAVDEGNSIKPEVILKEELMSEAATSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y026</accession>
    <name>A0A6I9Y026_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Forkhead-associated domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106547444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920097.1">
        <property type="nucleotide sequence ID" value="XM_014064622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y026"/>
    <dbReference type="GeneID" id="106547444"/>
    <dbReference type="KEGG" id="tsr:106547444"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920097.1"/>
        </source>
    </evidence>
    <sequence length="538" mass="60600" checksum="F611C68462E044A9" modified="2020-10-07" version="1">MEGQRALERTLDARVKESLGSLEDAKRGKATAEEKLSAWERRLQGLEAEAESLRQKHQREISEYQEQVKQHARTIVQLEKRLPSAATQPPEEERGPRPRETKKDPCKPPSPSPLGLCPMDGFHAFLKEELAEAKQEIQANQAVIAELKKELCQARATMSDVIGELSEKQKAELEEKRGLVQSQARELGLLREKLSESSRMLGQRENHLQATTEELKRAREKAKELLEEAKAAQRARLLQHKGVQTAGALQDQPHPAAKEPPLLSLADLGARCKGSRHEEVIHRQKETLAELRKKLRKLEKEARSSEPLLVVQRGPAEKPEQAREKDPVAVLTVAMGAHQPPAGPSSFDPNEATERNARLEMAEALDLSENLYLSLVRGLSSLMDVEQLMGLRTLKHLPQAERQKVGLLRHKDLDLLLDRIGKLKSRLERKESLLKEYEEEEQEQGGSGPFRGKRHSLQACQSEVARLSDQVHRDTEEKALLKEALERMRLQLSKEKRLNKALKRPKTPAPRKPPCESPKAAEGPREACRPRVPCGGWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0I3</accession>
    <name>A0A6I9Y0I3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipase maturation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LMF1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the maturation of specific proteins in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipase maturation factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919801.1">
        <property type="nucleotide sequence ID" value="XM_014064326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0I3"/>
    <dbReference type="GeneID" id="106547234"/>
    <dbReference type="KEGG" id="tsr:106547234"/>
    <dbReference type="CTD" id="64788"/>
    <dbReference type="OrthoDB" id="434126at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009613">
        <property type="entry name" value="LMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057434">
        <property type="entry name" value="LMF1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463">
        <property type="entry name" value="LIPASE MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463:SF10">
        <property type="entry name" value="LIPASE MATURATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06762">
        <property type="entry name" value="LMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase maturation factor 1/2 N-terminal" evidence="3">
        <location>
            <begin position="157"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013919801.1"/>
        </source>
    </evidence>
    <sequence length="410" mass="46345" checksum="69974131BEC2749E" modified="2020-10-07" version="1">MAASAGELRRRRGPAGDAPDPREPRAPPAPSLRPGSFWLTRLVLLRATAFIYSVAFLVAYHQNKALIGDRGLLPCKLFLENVKRHFKGRINMETLSYAPTLIWFLDWSSMDSILDAFALIGLAISAFVLVTGCANMILMTAVWIIYLSLINVGQIWYSFGWESQLLETGFLAIFLCPLWTFSRVAKDTPPSFIVIWAYRWLIFRIMLGAGMIKIRGDRCWKDLTCMNYHYETQPVPNPVAYFMHRSPWWFHAFETIFNHFIELVVPFFVFLGRRMCMAHGVLQILFQMLLIISGNLSFLNWLTIVPSIACFDDASLGILFGSRTGSLKSHILKIQAEEAAGKVGPLKYGSYIRKAVNVSFGALIIFLSIPVVLNLISSQQIMNTSYNPLRIVNTYGAFGRPMNRTNGSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y233</accession>
    <name>A0A6I9Y233_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Sesquipedalian-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC106547822</name>
        <name evidence="5" type="synonym">LOC106551708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920561.1">
        <property type="nucleotide sequence ID" value="XM_014065086.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920562.1">
        <property type="nucleotide sequence ID" value="XM_014065087.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925329.1">
        <property type="nucleotide sequence ID" value="XM_014069854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y233"/>
    <dbReference type="GeneID" id="106547822"/>
    <dbReference type="GeneID" id="106551708"/>
    <dbReference type="KEGG" id="tsr:106547822"/>
    <dbReference type="KEGG" id="tsr:106551708"/>
    <dbReference type="OrthoDB" id="10261837at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF53">
        <property type="entry name" value="INOSITOL PHOSPHATASE INTERACTING PROTEIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="24"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925329.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="23198" checksum="A0F766CCD5EA884E" modified="2020-10-07" version="1">MFQSQSATKLHIASILTSSRLEQPPDWQGVLFKKNNRTASYQHCWCELRGNLLICREQPGDRGPCHLIVLEGCRVELQESTSEPHTFKICYPDDLPDQSYKMAAEDQETMENWMRVLSMAGFENLRALVTELESQFHQLKSQQYSKEEASCKATERRFSDMDFACLHVEFGKDVNEARKKWQEGKRKHVPARENLMHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3R6</accession>
    <name>A0A6I9Y3R6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase 1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PPP1R3B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911755.1">
        <property type="nucleotide sequence ID" value="XM_014056280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3R6"/>
    <dbReference type="GeneID" id="106540962"/>
    <dbReference type="KEGG" id="tsr:106540962"/>
    <dbReference type="CTD" id="79660"/>
    <dbReference type="OrthoDB" id="8942186at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2440:FF:000001">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017434">
        <property type="entry name" value="Pase-1_reg-su_3B/C/D_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF13">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038207">
        <property type="entry name" value="PP1_GT_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 4" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="5">
        <location>
            <begin position="153"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911755.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="36038" checksum="FAD06E23F5073C04" modified="2020-10-07" version="1">MLQTGMQFRPNSFWVAGILDSFPQKPRMAVDVAMQFYLRAPSLRKESFAYKIAPKIKTPLRPCIHVNSSPVLKELGSEVQALRRNRVKKRVSFADSRGLALTVVKVFSEFDDRLDIPLNITELIDNIVGLTTVDHDDFVLDFIQPSADYLDFRNRLQADCVCLENCMLKDKAIVGTVKVRNLAFEKAVKIRMTFDTWKTFTDHSCQYVKDTYASSDKDTFSFDVSLPERVQAHERIEFAVCYECDGKVYWDSNKGFNYRIIRSELKSAREISQPCSETDFGIAFDQFGSPRCSYGLFPEWPSYSGYEKRGPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5P9</accession>
    <name>A0A6I9Y5P9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Poly [ADP-ribose] polymerase 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PARP9</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919998.1">
        <property type="nucleotide sequence ID" value="XM_014064523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5P9"/>
    <dbReference type="GeneID" id="106547380"/>
    <dbReference type="KEGG" id="tsr:106547380"/>
    <dbReference type="CTD" id="83666"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044389">
        <property type="term" value="F:ubiquitin-like protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060335">
        <property type="term" value="P:positive regulation of type II interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02907">
        <property type="entry name" value="Macro_Af1521_BAL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052056">
        <property type="entry name" value="Mono-ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453">
        <property type="entry name" value="PARP/ZINC FINGER CCCH TYPE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453:SF70">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Macro" evidence="8">
        <location>
            <begin position="46"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="8">
        <location>
            <begin position="244"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="7">
        <location>
            <begin position="609"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919998.1"/>
        </source>
    </evidence>
    <sequence length="855" mass="96365" checksum="F0556F02514604BB" modified="2020-10-07" version="1">MDEEMILIPISNSIYAVLKRRENYLCSLLQKKFGCICVLKSIRNPMEIYRKSLKEGVEVSVWVDDLTRHEADALVNAANEYLHHFGGLAFALLKAGGPEIEEQCKLFIEKNGPLSAGQIVVTSGGRLPCKQVIHAVGPRWSEDQKEECCLKLEAAIINVLKYVNAPENNIKSVAIPALSSGIFNFPQDWCAQVIVQTIRNFVQLAPLFGYLQEIHLVNIDETTADVMKRACEDLIGINDIVPQSSITDSITVNELCLQIKKGDIEAQQAAIIINSVTVQDGLFQGLVSRAILEKAGPAMQEQFLSELQKLPSERPNFIYTKGFNLDCEHVLHVVWPSYIEGSRQEVLKTAISKGLSKTGEQQLSSIAFPPLGIKDLHLPSNEVAEIMVEEIMHFAVEQTGKKLDVYIVISLDNSDVYEEFFKTLKSYEYHRSLENTLDVQLPVQDRNPHIIPDKGQPNAFCAKLMSVKNKLEEKMDCSNIDRMAELQTDVQANSGFERNGPFVELIGRSHDILEKAGQWLRDIILVEETGRIVIRNHNILNLRSVQPLELSHLQQHFGITILESVSGAGTALEIEGSPRAVIDAVFAIEFMLQSTAKQEPLKIDGSLESHLHQEGDLETANRCLYQITPIESYLQEFKDKEKQLEKAGLQVLKIEKIHNPLLESAFQRVKRKIEGRNTGMPVCHRLYHHVPAQYCSLVCKTGFQKTYSSSQGKPHTLQRKLLQSATHLYCYNKKYSKTACILYCFSDQKYGTGIYFKKNPRKLIADTREKCEMDHLICVFEAEVVTGSYTRGNRSYVAPPLINTSSMKLYDSVVDDIHNPEIFVIFNKEQALPLYLLTCSLLWSPDQKANLEPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6D1</accession>
    <name>A0A6I9Y6D1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel subfamily A member 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106547821</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920563.1">
        <property type="nucleotide sequence ID" value="XM_014065088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6D1"/>
    <dbReference type="GeneID" id="106547821"/>
    <dbReference type="KEGG" id="tsr:106547821"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000028">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000146">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF155">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="28"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013920563.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="55700" checksum="9EBC2CFF344303B9" modified="2020-10-07" version="1">MEKLLPLTTGESEPIVHCGGHSHKDCCERVVINVSGLRFETQAKTLKQFPDTLLGDPLRRLQYYDPLRNEYFFDRNRPSFDSILYYYQSGGRLRRPINVPLDVFLEELKFYQLGEEALSKFRDDEGFIKEEEQPMPDREFLKQVWLLFEHPESSQAARIIAIISVMVILISIVIFCLETLPEFRDETDMSLPLVHHHLNSSVPHIMPPKSHSEDPFFIVETICICWFSFELLVRFLACPSKPAFFKDIMNMIDFIAIIPYFVALGTEFARQRGVSQPAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADHEQSNFSSIPAAFWWAVVTMTTVGYGDMYPETIGGKIVGSLCAIAGVLTISLPVPVIVSNFSYFYHRETDCEDQGQYTHITTCPYTPPPSPELAAIQRKVHIHEKALHREKHGDKELTVPNSEFLDGMVPIGNSEAPNKRLVTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6D2</accession>
    <name>A0A6I9Y6D2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106547825</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920568.1">
        <property type="nucleotide sequence ID" value="XM_014065093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6D2"/>
    <dbReference type="GeneID" id="106547825"/>
    <dbReference type="KEGG" id="tsr:106547825"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="574"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="644"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="689"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="732"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="768"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="11">
        <location>
            <begin position="574"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013920568.1"/>
        </source>
    </evidence>
    <sequence length="978" mass="111353" checksum="C8BC23A42416EE49" modified="2020-10-07" version="1">MPSLIIPVFVHLFAFEVKHDPGIHGEYNPSFQFYIGKKKTDNETTISGAFRRWLHPFLRTAQFYNNSIDGVYLDETGELVADLGIMNTVVFSNLSFIKGTLGSLERVGSLDFKFMMNQNAIAKMEMMTKDILASLTGKCILTPMREEVHPLKNYKPGEFLIGGIVSATRMRLLPLSFNKPPLTQFVEKGSSEWWKMLSFLFAIQAINKDPNILLNVTLGYNIYDNYFRAEMTSEALLDLLSEGEANVPNYSCGRQRNIVAVLEGADTDISIQISTMLGPYKIPQISHSFDSQVLSDENPFFYPMLPGEKVLYPGIVKLLLHFRWTLVGLFTTDTENGETFMRMLTSMLVRNGICPVLSQTFSPVSWNAEIDWDLYLSWRKMKVFIYGADFSTFNSGVKLLHHIFEDLAEPPRGKLLITTIRWDFTIYLSKDTILVQYIHSIFSFPVKKNQMAKYGGFSKFYSFMRHFVEISFLWSYSNDDFSVKVWRQCRQREELEARRKEEMDPILTLDNYFIYNTIWAVARALNAAYLSRSSRISKGDAEKCTKCPEDQHPNTKRVHCIPKIKSYLSYQENLGIILTSIVLFLSLTTGFGLGIFIKFMETPIVKANNRDLSYILLVSLLLSILTSFLFIGRPRRVTCFIRQTTFSIVFSIAISSILAKTITVVLAFLATKPGNKVQRWLGKTLANSIVLSCSGLQVVICIIWLGTFPPFPDSDMNSQPREIILQCNEGSVTMFYVALGYMGFLSAVCFTVAFLARNLPGAFNEAKLISFSMLVFCSVWVSFVPAYLSTKGKYTVAVQLHSFLQSSQFYNNCIDGVYLDENGELIAELDIVNWVFFSNRSVNKVIFGSLERQGSLDFQLVLNQKAIAKTKVLNKLHAFLQNSQFYNNCIDGVYLDKNGELTAELDLVNWVIFSNRSVSKVTFGCLERQGSLDFSLMINQKSIAEVETLNKNTTFMLQIKISLVVLNRERFQIPEYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6R0</accession>
    <name>A0A6I9Y6R0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Meiosis-specific nuclear structural protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MNS1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. May play a role in the control of meiotic division and germ cell differentiation through regulation of pairing and recombination during meiosis. Required for sperm flagella assembly. May play a role in the assembly and function of the outer dynein arm-docking complex (ODA-DC). ODA-DC mediates outer dynein arms (ODA) binding onto the axonemal doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914215.1">
        <property type="nucleotide sequence ID" value="XM_014058740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6R0"/>
    <dbReference type="GeneID" id="106542888"/>
    <dbReference type="KEGG" id="tsr:106542888"/>
    <dbReference type="CTD" id="55329"/>
    <dbReference type="OrthoDB" id="197839at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026504">
        <property type="entry name" value="MNS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19265">
        <property type="entry name" value="MEIOSIS-SPECIFIC NUCLEAR STRUCTURAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19265:SF0">
        <property type="entry name" value="MEIOSIS-SPECIFIC NUCLEAR STRUCTURAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="15">
        <location>
            <begin position="116"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="109"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="251"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013914215.1"/>
        </source>
    </evidence>
    <sequence length="499" mass="61213" checksum="0D95A454F64FBF53" modified="2020-10-07" version="1">MAQKPRRRQPTAAQLMDRKQHELYFQQMRELEHRKKLERVHQLEVMWEAEDRVQQKRVTRLLREEEHEQRLEEAVRKAEESKRLKDLELQQEEKLATELARLNHDKLKDEKMRQQIKENSIELRELERKLKSAYMNKERAVQIAEKEALKYEKMKEDADIYRAMKEEQERAANAENAVETRRNQEKLLYQQELEKQLEEEERRKQAAYKEFLREKLLIDEIVRKIYEEDEKARQDRLEKKRTTQKFIEEFKKDQAMRRRRQREEMEEENQKIMEFANMWQEREDNRMAKIHENEEKKRKLQQMLTEVLKKEQQEREELEQIRAELHFEEQAETERKKEMEELEKRIRQRLELRGAYEEQLASRKMLLQALKEEEEAFGQALLAKFAEDDRIEQMNAQKRRMKQLEHRREVEKLIQERRKQVIADKEHELEEREIEERRRGIVAGIIEEERQKLLKEHAVKLLGYLPRGILKDERDVHMLGEEFRQAYQKRPGGGLSEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6W7</accession>
    <name>A0A6I9Y6W7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA transcription, translation and transport factor protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106542985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RTRAF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914345.1">
        <property type="nucleotide sequence ID" value="XM_014058870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6W7"/>
    <dbReference type="GeneID" id="106542985"/>
    <dbReference type="KEGG" id="tsr:106542985"/>
    <dbReference type="CTD" id="51637"/>
    <dbReference type="OrthoDB" id="514167at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019265">
        <property type="entry name" value="RTRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15924">
        <property type="entry name" value="CLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10036">
        <property type="entry name" value="RLL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914345.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="28055" checksum="02EF7676701025BB" modified="2020-10-07" version="1">MFRRKLTALDYHNPTGFNCRDETEFRNFIVWLEDQKIRHYKIEDRGNLRNIHNATEWSKSFEKYLKDVNCPFTIQERQEAIDWLLGLAVRLEYGDNADKYKDPTPEGSKNADNAAKNAEPLINLDVNNPDFKAGVMALANLLQIQRHDDYLVMLKAIRILVQERLSQDAVAKANQSKEGLPVTLDKHILGFDTGDAVLNEAAQILRLLHIEELRELQTKINEAIVAVQAIIADPKTDHRLGKVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y726</accession>
    <name>A0A6I9Y726_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Paladin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PALD1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the paladin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921208.1">
        <property type="nucleotide sequence ID" value="XM_014065733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y726"/>
    <dbReference type="GeneID" id="106548372"/>
    <dbReference type="KEGG" id="tsr:106548372"/>
    <dbReference type="CTD" id="27143"/>
    <dbReference type="OrthoDB" id="66369at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17660">
        <property type="entry name" value="PTP_paladin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000100">
        <property type="entry name" value="Phosphatase domain-containing paladin 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14566">
        <property type="entry name" value="PTPlike_phytase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01301">
        <property type="entry name" value="PTPlike_phytase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921208.1"/>
        </source>
    </evidence>
    <sequence length="878" mass="101305" checksum="461B40A2A51A07AC" modified="2020-10-07" version="1">MQRPFRCLWIMGTTASTAQQTVATATFENIHGSGTMEDHHSLSIHSFQTIGLHNNKAKSIITNKVAPVVITYNCKEEFQIHDDLMKASYTVGRITESTPEHYLVQGKYFMVRDVYGKADVLNTSSTCGAPNFRQAKGGYAVFGMGQPSFGGFKQVLQKLQNDGHKECIVFCIREEPVLFLRMDNDFIPYTPRAKENLKENLHNQHRGGKVEDLEVSIRKEIHDFAQLSENIYYVYNDIEHFKDEPHSIKIFCEEDIQVTEEVYKRPVFLLPSYRYHRLPFPVDGAPLETQFDAFISFLRESPNLLLLQDPSKPPPALVFSCQTGVGTTNFAMVLGTLVHYHRKGGLQKQDLPQSPKYSPREQFRIIQNFIGMVPNGQQIVEEVDAAIVLCSEMHDVKEAIYEYKRKLEGIGGDDQNQKNSTKECFLQKTLQRLERYFFLIAFNYYLHEQYPLAFALNFSRWICRHPELYRLQANMNLSELTVTAEHITKGVRVLVVDERFSPDVLSTVKDMNVANFRRVPKMPVYGMAQPNSKAIGNVLNYLTDAKRKHSHILWINLREDIVLEENEQTYTLREIGNLEQQITVPVASSEQLEKLESALKNDLLKSQKRIEVYLEQEKQMKTLKSCLTMQEIFNRHKSNCQGLAYKRIPVPDFCGLKEKDFDQILEAMKGALAEDSHTAFLFNCHSGRGRTTTAMVIAVLILWHFKGIPEITEDEIVSVPDAKYTKGEFEVVMKILQILPEGHRMKKEVDMALDTVSETMTPMHYHLREIIISTYRQGKTAKEEAEIQTLQLRSLQFLERYIYLILFNAYLHLAKNDSWVKPFSVWMHEVAAKAGVYEILNQLGFSEFETLDRQALSQLRYRWQEQSRSLLSFQGDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y748</accession>
    <name>A0A6I9Y748_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Catenin alpha-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106543175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914570.1">
        <property type="nucleotide sequence ID" value="XM_014059095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y748"/>
    <dbReference type="GeneID" id="106543175"/>
    <dbReference type="KEGG" id="tsr:106543175"/>
    <dbReference type="OrthoDB" id="9029550at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005916">
        <property type="term" value="C:fascia adherens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000007">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001033">
        <property type="entry name" value="Alpha_catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914">
        <property type="entry name" value="ALPHA CATENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914:SF21">
        <property type="entry name" value="CATENIN ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00805">
        <property type="entry name" value="ALPHACATENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914570.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="47236" checksum="13F1E2AF372AF706" modified="2020-10-07" version="1">MEMYKATWENHIRVLTEAVDDITSIDDFLGVSESHILEDVNKCIIALREQNADELDRAAGAIRGRAARVVDIVSGEMDNYETGAYTEGVMRNVRYLSNAVIPEFITQVNTALENLSRNMIHLFDDNQFVDISKKIYDTVHDIRCSVMMIRSPEELEDVSDLEEDHDVHSRTSIQTEGKTDRAKMTQLPEAEKEKIAEQVADFKKVKSELDKEIEIWDDTSNDIIVLAKRMCVIMMEMTDFTRGRGPLKNTSDVINAAKMISESGSRMDVLARLISNQCPDWSCKQDLLAYLEQIKLYSHQLKICSQVKAEIQNLGGELIMSALDSVTSLIQAAKNLMNAVVQTVKMSYIASTKIIRIQSPAGPRHAVVMWRMKAPEKKPLIKKEKPEETYAAVRKGSAKKKIHPIQIMSEFRGREIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y787</accession>
    <name>A0A6I9Y787_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KAT8 regulatory NSL complex subunit 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106543295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914745.1">
        <property type="nucleotide sequence ID" value="XM_014059270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y787"/>
    <dbReference type="GeneID" id="106543295"/>
    <dbReference type="KEGG" id="tsr:106543295"/>
    <dbReference type="OrthoDB" id="6022640at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035035">
        <property type="term" value="F:histone acetyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026180">
        <property type="entry name" value="NSL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029332">
        <property type="entry name" value="PEHE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22443:SF17">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22443">
        <property type="entry name" value="NON-SPECIFIC LETHAL 1, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15275">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01300">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52052">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEHE" evidence="2">
        <location>
            <begin position="45"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914745.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29107" checksum="9253EA43FA04777F" modified="2020-10-07" version="1">MASSSSSLVLPAKKKKVESSYDINNIVIPMSMAAATRVEKLQYKEILTPSWRTVDPKELVILEKADTELEDTSDETYLTYHQKFEELERARWDSWTGKSSQRRGNRSSSHKADGHWVPPPGCQDSLLNILHYPQGANSPTTSLSPDPFNVPMSLVLTGRGGNSFSEDSSFNTDEIQNVPPWEPRTFPLSNADYRALQDPSHKTFGQQWDKALRTNHINAAGLSHPREVLPAKHLMPPKDGDRQHQAEPCCFVTPTLNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7X8</accession>
    <name>A0A6I9Y7X8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">16 kDa beta-galactoside-binding lectin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106544249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915950.1">
        <property type="nucleotide sequence ID" value="XM_014060475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7X8"/>
    <dbReference type="GeneID" id="106544249"/>
    <dbReference type="KEGG" id="tsr:106544249"/>
    <dbReference type="OrthoDB" id="8443340at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013915950.1"/>
        </source>
    </evidence>
    <sequence length="103" mass="11843" checksum="6C8D5ED4E5436462" modified="2020-10-07" version="1">MDNVDQVEENYPSILKQDAEPGDCIKEKGKVELNAISLGQDETELILHFNLCFEFPFLSGEDIKLFVCFDAKEVTVKIPRSHKFKFPNRMEVVFFSIVGELEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9H9</accession>
    <name>A0A6I9Y9H9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein arginine N-methyltransferase 9</fullName>
            <ecNumber evidence="2">2.1.1.320</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein arginine N-methyltransferase 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PRMT9</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 17">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013918705.1">
        <property type="nucleotide sequence ID" value="XM_014063230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9H9"/>
    <dbReference type="GeneID" id="106546369"/>
    <dbReference type="KEGG" id="tsr:106546369"/>
    <dbReference type="CTD" id="90826"/>
    <dbReference type="OrthoDB" id="5980806at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000138">
        <property type="entry name" value="Protein arginine methyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000006">
        <property type="entry name" value="Protein arginine methyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000078">
        <property type="entry name" value="Protein arginine methyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000384">
        <property type="entry name" value="Protein arginine methyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF60">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8 12" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="12">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="14">
        <location>
            <begin position="742"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013918705.1"/>
        </source>
    </evidence>
    <sequence length="837" mass="94674" checksum="8E8CA822477F47A9" modified="2020-10-07" version="1">MPNTKIKSRHRHKVSQESGRRELVSRSLQSAQHCLENQDFGTAYAHYLLVLNIAPEFKNDVKETFQFTLFKWAEELDSLARIQDLFDCYEHALELYPNDEVIYNSMGEHLFRLGFRDEAAAYFHKAVKLNPDFPDAKENFYRVANWVVERWHFIMLNDYSRNFLYQKAIQKAVHSGYRSVLDIGTGTGILSMLAKKSGASPVYACELSKTMYELACEVLAANNLDGEIKLLHMKSFDIEIPKHIPERVSLVITETVDSGLFGEGIVESLIHAWEHLLLPPEPKNGKDNSKNYGKVIPAGATMWGMAVECKEIRRHHRVEVKEIAGVCLPETLKFCSPTSAPLKSSETLEPYTTEKLSRIPGGYTALTDIFQVLTVNFNNLQELKNLSTSKPSKINIPIIKDGILDAVVVWFTLHLDDELTLSTSPNVDTCWEQAVYPVQGLLDHSVKSGDCVAMEICCHDCYLRIQRISIKTPGYEMDVERNLKEKNSEAELCSALASLQTASTLENVCQECMLESTEIALLNNMQYHECFKIAIGKVLSSFSHHEESHSMDVVGHHHEFGKNQSKNSVTVDPLYILDVSEGFSILPVIAGKFGAVKAYSSVEKDHHQVALSLISEANQFPRETLEFWLRHLQDENKVLQRPKSDKLWNIIILDVIETSGLIQQDVMEKAAISRCLLHSGGKIFPDHVKIYGMLVESNLLFLESAVQGTDPTLGFNIAPFINQFKVPIRIFLDLSTLPYVPLSNSVELLRVDLMNPYFNNSSTEVEVEIRKSGQVTGIPFWYHVYLDNDIVLDTSSKSSHWKQAAIVLDKPIQVQEGEKLILSIQYYKSNISVVVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA89</accession>
    <name>A0A6I9YA89_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein phosphatase 1 regulatory subunit 3G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PPP1R3G</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920005.1">
        <property type="nucleotide sequence ID" value="XM_014064530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA89"/>
    <dbReference type="GeneID" id="106547386"/>
    <dbReference type="KEGG" id="tsr:106547386"/>
    <dbReference type="CTD" id="648791"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF7">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="225"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920005.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="38017" checksum="FABA7E48B825A8F7" modified="2020-10-07" version="1">METRGSAVLSLGQLFAPFGESQPNEEAALAPAWREEDAEEEEEEEEATAEAPAEENQQPERDCNAAAAREKDEEVLLEWRRRRPGRSFSLPDSPVRFFQRRLLPGDSDGSESDEDEDGGAAAASPTAALRGGGCCTKRVQFADALGLCLASVKHYSAAEEPQVPHAVLSRLQSFPMRQRDFEDFTAALAELGGCASAGSPFPRSPPQVPLRLAPPLDGADEPNGAERLRRDRVCLEEVAGTALGGAPTDVRGVARVLSCPGSKEVTVRYTFNEWLSFLDVPALPLPDGGDPSDGSPPVERYQFSLCLPPGLPNGTAVHFAICYRSQQGEHWDNNGGANYTLRDCSAEKPATPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAY4</accession>
    <name>A0A6I9YAY4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Potassium channel subfamily K member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KCNK9</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921230.1">
        <property type="nucleotide sequence ID" value="XM_014065755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAY4"/>
    <dbReference type="GeneID" id="106548398"/>
    <dbReference type="KEGG" id="tsr:106548398"/>
    <dbReference type="CTD" id="51305"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000057">
        <property type="entry name" value="Potassium channel subfamily K member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005407">
        <property type="entry name" value="KCNK9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF75">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038061">
        <property type="entry name" value="K_channel_subfamily_K_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01585">
        <property type="entry name" value="TASK3CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 15 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 14 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 14" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5 14" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="17">
        <location>
            <begin position="77"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="17">
        <location>
            <begin position="169"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013921230.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="42496" checksum="68BD24C6E609D5E2" modified="2020-10-07" version="1">MKRQNVRTLSLIICTFTYLLVGAAVFDALESEHELREEEQLKAEETRLKGKYNITSDDYQQLEEIIMQSEPHRAGVQWKFAGSFYFAITVITTIGYGHAAPGTNAGKAFCMCYAALGIPLTLVMFQSLGERMNTFVKYLLQRMKKCCRMRTTDVSMENMVAVGFFSCIGTLCIGAAAFSHCEEWTFFQAFYYCFITLTTIGFGDYVALQTKGALQRKPLYVAFSFMYILVGLTVIGAFLNLVVLRLFMNIEEERREAEERASVAGNRSSMVVHIQEDSQHGRPRYKAEVTDLQSDCSCMCYRSHEYTSRVVSHQNSFSSKLNLQYFHSISYKIEEISPSTLKNSLFPSPISSISPGLHSFTDHHRLMRRRKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBZ7</accession>
    <name>A0A6I9YBZ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein KIAA1958 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">KIAA1958</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921619.1">
        <property type="nucleotide sequence ID" value="XM_014066144.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013921620.1">
        <property type="nucleotide sequence ID" value="XM_014066145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBZ7"/>
    <dbReference type="GeneID" id="106548713"/>
    <dbReference type="KEGG" id="tsr:106548713"/>
    <dbReference type="CTD" id="158405"/>
    <dbReference type="OrthoDB" id="5957988at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042838">
        <property type="entry name" value="KIAA1958"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021893">
        <property type="entry name" value="ZMYM2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46963">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA E130308A19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46963:SF1">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA E130308A19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12012">
        <property type="entry name" value="DUF3504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="ZMYM2-like/QRICH1 C-terminal" evidence="5">
        <location>
            <begin position="517"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="231"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="337"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921620.1"/>
        </source>
    </evidence>
    <sequence length="732" mass="80697" checksum="6350516A83A47B74" modified="2020-10-07" version="1">MEDCLHTSSENLSKLVSWAHSHGTICSLIPNLKHLLSEGSHGNLTAMWGCSAGHAYHWPLTATCRAGSQERVCFQDNRSFNSDSPSIIGMPSDPQVSPVERYPGRSGKAKLDCNRTRDSCDFSYCSEPSELDETVEEYEDESNLFDMVCESSVTDEDSDFEPHHQRSQINSLKRSGPSSSLHLDSQSEVIDGSSSDVLAKKIKQELPEDYYIVANAEITGGIDGPALSLTHMSKPKGGTSYPVASKLSSHSSPSLSADLDLQNVSASSHVTSRPVTQKPPKLNLPSSSRRPGNIPTVNPQVALQVPASTSNSGKPISIPLSALQFPGQEELSASEDLLQPGPNQASNDVALSPSVSTEPEVSSSQQQPSTAPSAVTEPVAQTIADQDERAAELSREQNEKTIRSTQTALRNFREFLISKYPSETREIYIIPCKELDAYLASFFVDARQKDGSEYEPNSLANYQCGLERYLKEHRYAYSITRDKEFKRSQEALKQKQMELRCKGKGNKPHKSMKLTFADELILRKRGLLSRYNPEGLLNLVWLNNTKAFGHCTGFHGSTLKWGDIRLRVTETGLEYLEWMGQDGSDGNSKIKRTGTDSRVYATQHAPQTCPVQDYKEYAQRRPPGMRYEDAPFYLSIKPVVNLAALHWYNCQALGKNKLAKMVKTMCEKGNIPGRKTNFSVYQSCSTLSEAQSNQLVLICNNLSQQAAQSVASHSSTGNFIVSASYDSSSDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCS9</accession>
    <name>A0A6I9YCS9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 13 member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC13A2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921942.1">
        <property type="nucleotide sequence ID" value="XM_014066467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCS9"/>
    <dbReference type="GeneID" id="106548966"/>
    <dbReference type="KEGG" id="tsr:106548966"/>
    <dbReference type="CTD" id="9058"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015139">
        <property type="term" value="F:alpha-ketoglutarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015138">
        <property type="term" value="F:fumarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017153">
        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071285">
        <property type="term" value="P:cellular response to lithium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031312">
        <property type="entry name" value="Na/sul_symport_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01271">
        <property type="entry name" value="NA_SULFATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013921942.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="44953" checksum="E332BC8E865EEF02" modified="2020-10-07" version="1">MDPSPSHGPPGNTHVQVVVKEKLPEEAEQKLPEEDRQQLLKKHQTLSKGMSLAVCYSASIGGIATLTGTTPNLVMKGQMDELFCRNDNVVNFASWFTFAFPAMLVLLVLSWLWLLVMFLGVDFKKNFGCGSSEAEKQKLDQAYSIIRSEYRALGRMKFSEGAVLALFIVLVVLWFTREPGFMPGWANALFSQKGKSHITDATVVIFISLLMFVIPSELPSCRKAQQEGGSGQIRAPPALLDWTTVNRHMPWNIVILLGGGFALAKGSEVSGLSGWLGDKLTPLQSIPHPAITFILCLLVAVFTECTSNVATTTLFLPILASMSQAIELNPLYVMLPCTLAASLAFMLPVATPPNAIVFSYGNLRVIDMVKAGFMLNILGVLTIMLAINTWGIPMFNLDQFPAWAHANATQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCY1</accession>
    <name>A0A6I9YCY1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquitin domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">UBTD1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922013.1">
        <property type="nucleotide sequence ID" value="XM_014066538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCY1"/>
    <dbReference type="GeneID" id="106549024"/>
    <dbReference type="KEGG" id="tsr:106549024"/>
    <dbReference type="CTD" id="80019"/>
    <dbReference type="OrthoDB" id="1640476at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd17120">
        <property type="entry name" value="Ubl_UBTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.225.20">
        <property type="entry name" value="Ub domain-containing protein, DC-UbP/UBTD2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032752">
        <property type="entry name" value="DC-UbP/UBTD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038169">
        <property type="entry name" value="DC-UbP/UBTD2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039869">
        <property type="entry name" value="UBTD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13609">
        <property type="entry name" value="UBIQUITIN DOMAIN CONTAINING 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16455">
        <property type="entry name" value="UBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="149"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013922013.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="25789" checksum="020FB3372358BF74" modified="2020-10-07" version="1">MGSCVGRQRREGPITPGNPRKRAGRNEPLKKDRPKWKSDYPMTDGQLRSKRDEFWDTAPAFEGRKEIWDALKAAAYAVEANDHGLAQAIIDGAGVTLPHGSLTECYDELGNRYQLPVYCLAPPINLIMEWSEEEGAEPPEPAPNTRREFPLKVRLSSGKDLRLNASMTDTIGQLKKQLYAQEELEPAWQRWFFSGKLLTDRTRLQETKIQKDFVIQVIVNQPSAPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDG9</accession>
    <name>A0A6I9YDG9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Membrane-associated progesterone receptor component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PGRMC1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922211.1">
        <property type="nucleotide sequence ID" value="XM_014066736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDG9"/>
    <dbReference type="GeneID" id="106549162"/>
    <dbReference type="KEGG" id="tsr:106549162"/>
    <dbReference type="CTD" id="10857"/>
    <dbReference type="OrthoDB" id="547796at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF23">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0492">Microsome</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013922211.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16085" checksum="E9F6D2D531822D74" modified="2020-10-07" version="1">MAVDEAGAGGGAAAAAEGSEAASPGLLMEIVSSPLNLSLLGLCLFLLYQIVRGDRPRAQLEGEEEPPPPPKLKRRDFTLAQLRPFDGQTDPHILMAINGKVFDVSRGRKFYGPVKYHYVGKLLKDGEEPTVYSDEEETKDEKDRKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDM6</accession>
    <name>A0A6I9YDM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Inactive phospholipase C-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922271.1">
        <property type="nucleotide sequence ID" value="XM_014066796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDM6"/>
    <dbReference type="GeneID" id="106549210"/>
    <dbReference type="KEGG" id="tsr:106549210"/>
    <dbReference type="OrthoDB" id="9422881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922271.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="21932" checksum="F75473BB55768BC3" modified="2020-10-07" version="1">LFFPPYFSPQNSILSVKELCGLPPITSLKQCILTLVSRLVNNDSVPSVALCMKDNFPYLEPLGTLTDVQKKVLAAYDQMIQESKLLVETADTVYDKIIQCQKAGMELHEELHNLGTKEGLKGRKLSKAIESFAWNITVLKGQGDLLRNAKNEAIENMKQIQLACLSRGLSKSGSGNSDAKAKPGPEPAEKKETSGENARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDV2</accession>
    <name>A0A6I9YDV2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L39</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RPL39</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922219.1">
        <property type="nucleotide sequence ID" value="XM_014066744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDV2"/>
    <dbReference type="SMR" id="A0A6I9YDV2"/>
    <dbReference type="GeneID" id="106549168"/>
    <dbReference type="KEGG" id="tsr:106549168"/>
    <dbReference type="CTD" id="6170"/>
    <dbReference type="OrthoDB" id="6332053at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020083">
        <property type="entry name" value="Ribosomal_eL39_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970">
        <property type="entry name" value="RIBOSOMAL PROTEIN L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00051">
        <property type="entry name" value="RIBOSOMAL_L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013922219.1"/>
        </source>
    </evidence>
    <sequence length="51" mass="6379" checksum="2023CA9B5FFB982C" modified="2020-10-07" version="1">MSSHKTFKIKRFLAKKQKQNRPIPQWIRMKTGNKIRYNSKRRHWRRTKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEJ8</accession>
    <name>A0A6I9YEJ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 12-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED12L</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922603.1">
        <property type="nucleotide sequence ID" value="XM_014067128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEJ8"/>
    <dbReference type="GeneID" id="106549468"/>
    <dbReference type="KEGG" id="tsr:106549468"/>
    <dbReference type="CTD" id="116931"/>
    <dbReference type="OrthoDB" id="9049855at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021989">
        <property type="entry name" value="Mediator_Med12_catenin-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF3">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12144">
        <property type="entry name" value="Med12-PQL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit Med12 catenin-binding" evidence="2">
        <location>
            <begin position="234"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013922603.1"/>
        </source>
    </evidence>
    <sequence length="551" mass="62335" checksum="6A218F20A84747CD" modified="2020-10-07" version="1">MLSVLINGTLASDLSIASQMGSEENKRAYMNLVKKLKKELGDKSSESIDKVRQLLPLPKQMCDLIACEPMGSLIDTKGNKIAGFDSIDKKQGLQVSMKQKVSPWDLLEGSKNPAPLSWAWFGTVRIDRKVIKYEEQQHLLLYHTHPKPKPRSYYLEPLPLPPEEEEEEEEPTTPASQEPERKSAELSDQGKPSAEEERKGKGRKRKTKAASKTDEYPQNNGCRMAPNFSPLYSQMLHHPQSALWGYNVIGQPQQSSYFVQNQPLPPGGSRLDPTGSFVPSNTKQVLSNMLQRRSSNMMQPPAIHTLSSHHQQQQLLQQQWILRHQSQGPTIEQASIFAQQVRPSSSQLTQYPGIQQSQTIPHGYTMYSTQMSLPSQQTGGLVLSPTYNPRSFQATHSSPVLMERLRQMQQQPSGYIHQQAASYLQPLAGNQRSVHQSLQQNNLVGGPLDAVSATSAQANLYSVQVSQEQMRQRQQQIRQQRFLQLQQQQNQQQIISLQAMQPQQQSFFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGMNPYSHLPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEM9</accession>
    <name>A0A6I9YEM9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Chordin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CHRDL1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922621.1">
        <property type="nucleotide sequence ID" value="XM_014067146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEM9"/>
    <dbReference type="GeneID" id="106549479"/>
    <dbReference type="KEGG" id="tsr:106549479"/>
    <dbReference type="CTD" id="91851"/>
    <dbReference type="OrthoDB" id="8173378at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045717">
        <property type="entry name" value="CHRDL1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045716">
        <property type="entry name" value="CHRDL_1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46303:SF2">
        <property type="entry name" value="CHORDIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46303">
        <property type="entry name" value="VWFC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19548">
        <property type="entry name" value="CHRDL_1_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23334">
        <property type="entry name" value="VWC2L_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026853426" evidence="8">
        <location>
            <begin position="26"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="9">
        <location>
            <begin position="33"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="9">
        <location>
            <begin position="110"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="9">
        <location>
            <begin position="254"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="193"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922621.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="50237" checksum="8AD10D0D41820F4F" modified="2020-10-07" version="1">MWNLEHIRLICICLLCLIFTEESKTEPGKYSETYCMFQAKKYQIGQRWNPYLEPHGLVPCLECRCTEGGNVLCMKVKCATLRCSNQVFEPTKCCPKCPDPSVASNITTGKACNYNDTIYQHGEMFSMEGVFKGKQPNQCWQCICSEGKVYCGLKTCPNLTCTSPVSTSDSCCPVCKDEESLRVLGGDLLRQPAIRGARHSPPSQPETSTSNPRLPTINPQSRTGRANLRPLPNITQSLGTFVWTLINNRQEVGQVCISNGKTYSQGEIWHPVLRLHGTVECVLCTCKGTKPECKTIQCPYQYPCEHPKKIKGQCCKVCPEDIESQLPDNFCGEETFPVYEGILDSFEGTVQKIALEKNETSEVEIYTWTTKRGILIKSHNTMPMEEFRQLSYFQQITRTTLNKWEIFSEEAAQVNHCENRDCRTELEELLKVLYLEKLEQEGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFC7</accession>
    <name>A0A6I9YFC7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger CCCH domain-containing protein 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ZC3H18</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922938.1">
        <property type="nucleotide sequence ID" value="XM_014067463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFC7"/>
    <dbReference type="GeneID" id="106549743"/>
    <dbReference type="KEGG" id="tsr:106549743"/>
    <dbReference type="CTD" id="124245"/>
    <dbReference type="OrthoDB" id="10072532at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052647">
        <property type="entry name" value="Zinc_finger_CCCH-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46582">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46582:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="404"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="409"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="571"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="633"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="686"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="709"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="787"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="811"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="826"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="844"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="872"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="942"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013922938.1"/>
        </source>
    </evidence>
    <sequence length="1002" mass="111947" checksum="1926E9A16AA38060" modified="2020-10-07" version="1">MDVSETLEQNPRSPSEKDEEEDDHQPSDDENLKGSSSEQDGEEGRGASLGKGPNRATRTLSHGEEEGLPSEEEERLSETKSPDSDRNDQSQPLKSSLRVEQVKEGANDLGDEASSVIRELDEHELDYDEEVPEEPSTVPADDEADKAVGEEEEEEEEEKGDEPPGDDKQPTPKSNSEKEGQEPPKEKKKEEDGEIDDGEIDDDDLEEGEVKDPNDRKMRPRLTCRFFVKGNCTWGINCRFIHPGVNDKGNYSLITKPVPFSSNGAPPIGPGPHPLMPANPWGGPGVDEIFPPPPPDPPTESAWERGLRHAKEVLKKATIRKEQEPDFEEKRFTVTIGEDENEFDKENEFLRDWNYRITRDVRETSDTDVKENANFGLDPYADPYYDYEIERLWRGGQYENFRVQYTDPDPYRNYRLSKERERERDRENRQRERERERERERERERRQRERERERERERDKERQRRKEEWERERERIKRDDKERPHRDRDREREKEREKEKEKPKPRSPQPASRQPEPLKKEKEATTTTTTTPSHSKRADEWKDPWRRSKSPKKKHGMSASPSHARRRRKASASSASGSGSSRSSSRSSSYSGSGSSRSRSRSSSYSSYTSRSSRHSSFSGSRSRSQSFSSSPSPSPTPSPHKPPPKVKGESAVLAGKGEKIVKKLPAPPAPPPVAKVAPSAPEPAKPGDNREARRKERQTRTPPRRRAVSCSGSGSSYSGSSGSRSLSRSLSHSRSRSSSASLSPSPSGSRKSRSLSVSSVSSASSASSSSSSIRSADSEDMYADLASPVSSASSRSPTPAQQKKERGKAKKESGPKEEKRKRDVPAQPAKSAKPASGGKSQQPPAPHPPPQAQPAGFSAHKEIKLTLLNKAADKNSSRKRYEPSDKDRQASPPTKRINLSPGIRDRKLAGRLSSPKQDRQRGPNPKPSPAQADKKRPLSPPSKSSSKVTSIPGKAAEPAPPAAPAKSGKSSTLSRREELLKQLKAVEDAIARKRAKIPGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH96</accession>
    <name>A0A6I9YH96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Nucleic acid dioxygenase ALKBH1</fullName>
            <ecNumber evidence="24">1.14.11.33</ecNumber>
            <ecNumber evidence="23">1.14.11.51</ecNumber>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Alkylated DNA repair protein alkB homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Alpha-ketoglutarate-dependent dioxygenase ABH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">DNA 6mA demethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">DNA N6-methyl adenine demethylase ALKBH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">DNA lyase ABH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">DNA oxidative demethylase ALKBH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">mRNA N(3)-methylcytidine demethylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">ALKBH1</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="36">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>5-methylcytidine(34) in mitochondrial tRNA(Met) + 2 2-oxoglutarate + 2 O2 = 5-formylcytidine(34) in mitochondrial tRNA(Met) + 2 succinate + 2 CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:54144"/>
            <dbReference type="Rhea" id="RHEA-COMP:13808"/>
            <dbReference type="Rhea" id="RHEA-COMP:13809"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:138075"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(1)-methyladenosine(58) in tRNA + 2-oxoglutarate + O2 = adenosine(58) in tRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:79019"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a methylated nucleobase within DNA + 2-oxoglutarate + O2 = a nucleobase within DNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:30299"/>
            <dbReference type="Rhea" id="RHEA-COMP:12192"/>
            <dbReference type="Rhea" id="RHEA-COMP:12193"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:32875"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="1.14.11.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an N(1)-methyladenosine in tRNA + 2-oxoglutarate + O2 = an adenosine in tRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54576"/>
            <dbReference type="Rhea" id="RHEA-COMP:10242"/>
            <dbReference type="Rhea" id="RHEA-COMP:12312"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>an N(3)-methylcytidine in mRNA + 2-oxoglutarate + O2 = a cytidine in mRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:60920"/>
            <dbReference type="Rhea" id="RHEA-COMP:15145"/>
            <dbReference type="Rhea" id="RHEA-COMP:15713"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74894"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:60921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>an N(6)-methyl-2'-deoxyadenosine in DNA + 2-oxoglutarate + O2 = a 2'-deoxyadenosine in DNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:49524"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="1.14.11.51"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="33">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="33">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Monomer. Interacts with DNAJB6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.33" evidence="24"/>
    <dbReference type="EC" id="1.14.11.51" evidence="23"/>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013923566.1">
        <property type="nucleotide sequence ID" value="XM_014068091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH96"/>
    <dbReference type="GeneID" id="106550227"/>
    <dbReference type="KEGG" id="tsr:106550227"/>
    <dbReference type="CTD" id="8846"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141131">
        <property type="term" value="F:DNA N6-methyladenine demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035515">
        <property type="term" value="F:oxidative RNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035513">
        <property type="term" value="P:oxidative RNA demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141137">
        <property type="term" value="P:positive regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990983">
        <property type="term" value="P:regulation of translational initiation by tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000006">
        <property type="entry name" value="AlkB homolog 1, histone H2A dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557:SF2">
        <property type="entry name" value="NUCLEIC ACID DIOXYGENASE ALKBH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 33" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 33" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0692">RNA repair</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Alpha-ketoglutarate-dependent dioxygenase AlkB-like" evidence="34">
        <location>
            <begin position="94"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00052237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00063554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00066586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00066725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00071276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00076476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00076973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00077991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00079723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00080338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00080514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00081604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRSR" id="PIRSR604574-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="Pfam" id="PF13532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="RefSeq" id="XP_013923566.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="41283" checksum="CB5D22D59B76F3EE" modified="2020-10-07" version="1">MAAATREEDAFRRLFRFYRRWEGSDPSGVIDFSKPAGQQVVTYPLKLTSVSDQDAYRAGLHPVTQWKAYGLHNYPGFIYINNPFLPGSQCHWVKQCLKKYIQKPNVCNLDMHMSSEEIADIWGKSKFQLRNKSASKSQPKSLLEKLRWVTLGYHYNWNTKKYSAENYTPFPSDLAFLSKQVAKACGFPSFQAEAGILNYYHFDSSLGIHVDESELDLTPPLLSFSFGQSSIFLLGGLKRDETPAALFTHSGDIMIMSGFSRLLYHAVPKILPNLEGKPLPSSLELPLSAEFPADSVIELCSDDDWKTCARYLEASRINMTVRQVLAEGQSFLTETRTESKQNAFSTDYHGKYTETITHKPIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHJ8</accession>
    <name>A0A6I9YHJ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcriptional repressor scratch 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Scratch homolog 1 zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106550310</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923671.1">
        <property type="nucleotide sequence ID" value="XM_014068196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHJ8"/>
    <dbReference type="GeneID" id="106550310"/>
    <dbReference type="KEGG" id="tsr:106550310"/>
    <dbReference type="OrthoDB" id="5428132at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000043">
        <property type="entry name" value="Scratch family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000169">
        <property type="entry name" value="transcriptional repressor scratch 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000207">
        <property type="entry name" value="zinc finger protein SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF48">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR SCRATCH 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013923671.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="26544" checksum="1C8E41792B740B55" modified="2020-10-07" version="1">AGYIPACLPSSGYKNEKKKAHRRGASPDPLYTPIQEDYSDPESPQSSLSSHYFQGEAAVTESYSMDAFFIIDGRSRRRAEGPRHGAHRHACTECGKTYATSSNLSRHKQTHRSLDSKLARKCPTCSKAYVSMPALAMHLLTHNLKHKCAVCGKAFSRPWLLQGHMRSHTGEKPFGCSHCGKAFADRSNLRAHMQTHSALKHYRCKQCHKTFALKSYLHKHYESACFKGPEHACPAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHQ8</accession>
    <name>A0A6I9YHQ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein ZBED8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106550353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923726.1">
        <property type="nucleotide sequence ID" value="XM_014068251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHQ8"/>
    <dbReference type="GeneID" id="106550353"/>
    <dbReference type="KEGG" id="tsr:106550353"/>
    <dbReference type="OrthoDB" id="1101576at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF19">
        <property type="entry name" value="LOW QUALITY PROTEIN: ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013923726.1"/>
        </source>
    </evidence>
    <sequence length="190" mass="21431" checksum="915B0EE3743B231A" modified="2020-10-07" version="1">MVFGTLTSSQLFLQNKSPAPLPHGEFEVLLYHSNVRWLSRGQVLNRVFAVRVELALFLQEHQHCHADCFKNSEFILILAYMADIFAALNHLNQQMQGGGVNIIEAEENLKAFQKKLPLWKRRIENDNFANFPLLDDCVSKIEDVSGIRDISVPAELKQAIALHLDSLAKSLDGYFPTRVISSMGETAVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI93</accession>
    <name>A0A6I9YI93_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Voltage-dependent L-type calcium channel subunit beta-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcium channel voltage-dependent subunit beta 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Calcium channel voltage-dependent subunit beta 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Voltage-dependent L-type calcium channel subunit beta-4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CACNB4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Increases CACNA1B peak calcium current and shifts the voltage dependencies of channel activation and inactivation. Increases CACNA1C peak calcium current and shifts the voltage dependencies of channel activation and inactivation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1C) and the ancillary subunits CACNB3 and CACNA2D1. The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio. Interacts with CACNA2D4. Interacts with FASLG. Interacts with CBARP; prevents the interaction of CACNB3 with the alpha subunit CACNA1C thereby negatively regulating the activity of the corresponding calcium channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The L-type calcium channel is composed of four subunits: alpha-1, alpha-2, beta and gamma. Interacts with FASLG. Interacts with CBARP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923927.1">
        <property type="nucleotide sequence ID" value="XM_014068452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI93"/>
    <dbReference type="GeneID" id="106550522"/>
    <dbReference type="KEGG" id="tsr:106550522"/>
    <dbReference type="CTD" id="785"/>
    <dbReference type="OrthoDB" id="5962384at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12043">
        <property type="entry name" value="SH3_CACNB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000432">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000015">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046937">
        <property type="entry name" value="CAB1-4_N_A-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008079">
        <property type="entry name" value="VDCC_L_b3su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000584">
        <property type="entry name" value="VDCC_L_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11824">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01696">
        <property type="entry name" value="LCACHANNELB3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="58"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="399"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013923927.1"/>
        </source>
    </evidence>
    <sequence length="486" mass="54709" checksum="9322CAA6543DB645" modified="2020-10-07" version="1">MYDNLYLHGFEDSEAGSADSYTSRPSDSDVSLEEDREAIRQEREQQAAIQLERAKSKPVAFAVKTNVSYCGALDEDVPVPSTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLRLENIRIQQEQKRGRFHGGKSSGNSSSSLGEMVSGTFRATPTTTAKQKQKVTEHVPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDADTINHPAQLIKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDVILDENQLEDACEHLAEYLEAYWRATHTTSSTPMTPLLGRNLGSTALSPYPTAISGLQSQRLRHSNHSTENSPIERRSLMTSDENYHNERARKSRNRLSSSSQHSRDHYPLVEEEYSDSYQDSYKPQRNRGSPGGYSHDSRQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIF6</accession>
    <name>A0A6I9YIF6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein FAM160A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FAM160A1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FHIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923986.1">
        <property type="nucleotide sequence ID" value="XM_014068511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIF6"/>
    <dbReference type="GeneID" id="106550573"/>
    <dbReference type="KEGG" id="tsr:106550573"/>
    <dbReference type="CTD" id="729830"/>
    <dbReference type="OrthoDB" id="6287422at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019384">
        <property type="entry name" value="FHIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045669">
        <property type="entry name" value="FHIP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045668">
        <property type="entry name" value="FHIP_KELAA_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705:SF6">
        <property type="entry name" value="FHF COMPLEX SUBUNIT HOOK-INTERACTING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705">
        <property type="entry name" value="RAI16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19314">
        <property type="entry name" value="DUF5917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19311">
        <property type="entry name" value="KELAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10257">
        <property type="entry name" value="RAI16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHF complex subunit HOOK-interacting protein C-terminal" evidence="3">
        <location>
            <begin position="816"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="579"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="776"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="587"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923986.1"/>
        </source>
    </evidence>
    <sequence length="1007" mass="113087" checksum="65034DC13784B3DF" modified="2020-10-07" version="1">MSTGTSGKKSHQAVSLEGVDPETCMIVFKTHWAQVVKILETHDPLKNIQAKYGTISPDEASTVQNYVEHMLFLLIEEQAKDAAMGPILEFVVTENIMEKLFLWSLRREFTDEMKIEQLKMYEMLVTQSHQQLLHHKPILKPLMMLLSSCSGTTTPAVEVELVVLLNQLCSILAKDPSILELFFHTSEDQGAANFLIFSLLIPFIHREGTVGQQARDALLFIMSLSSESYVVAHHIVENTYFCPVLATGLSGLYSSLPTKLEDNGEEWHCLLKDDCLLSPALVQFMNSFEFCNAVIQVAHSLIRNQLVNYIYNGFLVPVMAPALHKVTVEEVMATTAYLDLFLRSVSEPALLKIFLSFILLHRHENVHILDTLASRINTPFQLCVVSLALFKTLISLHCEDVMLQLVLRYLIPCNHIMFSQRWAVRERDWYSVSAAKLLALTPVCCSSGITLTFEKQEKDYILWKKNNNGEDSTEEPSRKSIADSACIVKYGKAIDISYLQYLWDAQTLILHCMKDCEVWSAPYDGENPNPYTFIQTSVEEDGKNLNQPIFQLQPRTPRNHVSSQARPLRQKIQLELEWDDSYDTGISPDSDTGSPQRNDEFNTQPPAEPPKHIQEMKKNAIMLIKGSYIEESDFQDDVMVYRLCAKKDAQEAECLKGKPLISESADVDNPDAISTNGPLESPPSDLNLEECNMKNLNLIQQAKDETPGNIDETTLQPDLEQTLSSPEGDHNTKLLSPDSEDFISECDNIIKELDSNSTGLVELKLLIPDPVSPIEEEEDFENFSADTPSAESVPSPFGLKDAWPSNPARVQNIPFTGPFISVVLSKLENMLENSLHVNLLLIGIITHLASYPQPLLRSFLLNTNMVFQPSVRSLYQVLASIKNKIEQFASIEKDFPALLIQAQKYLLLRVDALDVPLESLNKDNGQQKSVTEKVKNLSEASQALLQTYLGNKARKGSGLPNVPLNIPNSILAAALFPEFLKELAALAQEHSILSYKVLGNLEDYYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK42</accession>
    <name>A0A6I9YK42_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Calfacilitin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TLCD1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924526.1">
        <property type="nucleotide sequence ID" value="XM_014069051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YK42"/>
    <dbReference type="GeneID" id="106551044"/>
    <dbReference type="KEGG" id="tsr:106551044"/>
    <dbReference type="CTD" id="116238"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071709">
        <property type="term" value="P:membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097035">
        <property type="term" value="P:regulation of membrane lipid distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF5">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="39"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924526.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="14777" checksum="DC01AD3653CA51A7" modified="2020-10-07" version="1">MAPWLRSAGLVAGSSLAFAALRWALSRCPLPAHVRRDATRTWRWKNLLVSFAHSVVAGLWAVVGMWQLPGALDDLVQTTSPSAHLLLCFSTGYFIHDSLDIIICRQSKASWEYLVHHAVASGERGGGLWGRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YL14</accession>
    <name>A0A6I9YL14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106551279</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924821.1">
        <property type="nucleotide sequence ID" value="XM_014069346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YL14"/>
    <dbReference type="GeneID" id="106551279"/>
    <dbReference type="KEGG" id="tsr:106551279"/>
    <dbReference type="OrthoDB" id="405848at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000019">
        <property type="entry name" value="Bc1_11K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="13">
        <location>
            <begin position="26"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="35"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013924821.1"/>
        </source>
    </evidence>
    <sequence length="89" mass="10400" checksum="309A2388B441E69C" modified="2020-10-07" version="1">MGREDEKAIEGRRDDPEEEEEEELVDPLTIVREECAKIEECAKALERLEQCNSRVSSRSQTLEECTEELFDFLEARDHCVAHKLFSKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLH6</accession>
    <name>A0A6I9YLH6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytokine receptor-like factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CRLF1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924991.1">
        <property type="nucleotide sequence ID" value="XM_014069516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLH6"/>
    <dbReference type="GeneID" id="106551408"/>
    <dbReference type="KEGG" id="tsr:106551408"/>
    <dbReference type="CTD" id="9244"/>
    <dbReference type="OrthoDB" id="9404142at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000386">
        <property type="entry name" value="Cytokine receptor like factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027816">
        <property type="entry name" value="MAJIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF28">
        <property type="entry name" value="ERYTHROPOIETIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25552">
        <property type="entry name" value="LIFR_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15077">
        <property type="entry name" value="MAJIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="119"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013924991.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="33251" checksum="FB5979C3AE1DA979" modified="2020-10-07" version="1">MDIERTTKQRRLGDILETSATQHYVKRLDAIRVVLGNLDSLCPFTTEHLIIFPLPPEKPTNISCWSKNLKDLTCRWVPGSEGETHLHTNYSLKYKLRQFFLTWYQSKQDCDLYAVTTDPPSDVHVSRVGDLEDQLSVRWSAPPALKDFLFQAKYQIQYRVEDSSEWKVVDNVSNQTSCRLAGLRPGTVYFVQIRCNPVGIYGSKKAGIWSDWSNPTAASTPRNGRTPGVCEPKNGEHNNTLRRELKQFFGWMRKHGTCANLSIKMYDQWRVALQKSHKTRNQVLSSDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLU7</accession>
    <name>A0A6I9YLU7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Junctophilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">JPH2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the junctophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925106.1">
        <property type="nucleotide sequence ID" value="XM_014069631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLU7"/>
    <dbReference type="GeneID" id="106551510"/>
    <dbReference type="KEGG" id="tsr:106551510"/>
    <dbReference type="CTD" id="57158"/>
    <dbReference type="OrthoDB" id="284854at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030314">
        <property type="term" value="C:junctional membrane complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000001">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000003">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017191">
        <property type="entry name" value="Junctophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085:SF26">
        <property type="entry name" value="JUNCTOPHILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037387">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="719"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="445"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="592"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="635"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="542"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="635"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925106.1"/>
        </source>
    </evidence>
    <sequence length="741" mass="81255" checksum="16BBF62D13B63DDD" modified="2020-10-07" version="1">MSGGRFDFDDGGAYCGGWEGGKAHGHGICTGPKGQGEYSGSWNYGFEVVGVYTWPSGNTYEGYWSQGKRHGLGIETKGRWFYKGEWTHGFKGRYGIRQNVSNGAKYEGTWNNGLQDGYGTETYADGGTYQGQFTNGMRHGYGVRQSVPYGIAAVVRNPLRTSLTSLRSEHSNGTLLQHDLPSANQEHVPMSPTITRGGFALSLYADADAGKLKKGGIFKRSSLFGKIKKSDSKTSLASQRSRLSFLKSESGMSSTGSDATSTASLGEGVEGEDFPPFDADIDATTTESYMGEWKNDKRSGFGISERSSGLKYEGEWLDNVRHGYGCTTLPDGKKEEGKYRMNVLIKGMKKRVIPLKSAKIRQKVDRNIEGAQRAAAIARQKSEIAASRTAHAKAKSEGAEQSAIAANNESMLARSMARELSPDFYQPGPEYLKRRLLQELMENEENVEHIQETPPVEETSPATPPESPQGDEQEFMKPKESPAPTPSPSGTPPEAKRAKQGSQKDGFLSPGNWNGDHTKNGNYTGSRPNTSSSVLLAPAEKSGSNIRSASQSPSHPSHMKIATDRMEIKPLERIQKEGEVKLFEGYHSYAVRTSPVTPPLDNEEEFSDSTFPTMSKSLEPESDVKAVDHKLELEKRESTPVTHHEPGHKEKKIIKTESKLEPKQHPPEPKVEEKVEAVSETKPEAKMVAKTEPKTLVKKVSPPEAATKEVVEKEEGPNTIIICMVILLNIGLSILFVHFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNG2</accession>
    <name>A0A6I9YNG2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heterochromatin protein 1-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HP1BP3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925285.1">
        <property type="nucleotide sequence ID" value="XM_014069810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNG2"/>
    <dbReference type="GeneID" id="106551669"/>
    <dbReference type="KEGG" id="tsr:106551669"/>
    <dbReference type="CTD" id="50809"/>
    <dbReference type="OrthoDB" id="7684689at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000228">
        <property type="entry name" value="heterochromatin protein 1-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832:SF1">
        <property type="entry name" value="HETEROCHROMATIN PROTEIN 1-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832">
        <property type="entry name" value="SHC (SRC HOMOLOGY DOMAIN C-TERMINAL) ADAPTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="H15" evidence="9">
        <location>
            <begin position="155"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="H15" evidence="9">
        <location>
            <begin position="253"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="H15" evidence="9">
        <location>
            <begin position="336"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925285.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="47812" checksum="83960EA203941844" modified="2020-10-07" version="1">MATELSEADPAHRKAVPLLTGAPLDKLSETVEDRAMPIRRAVNASSRETPPKSKPAEAEETKPEPDVSSEESASTVEEQENQVPPAAPAETEQPKEAEEEKADVKPAEEAKKEEKDPEKEKEKEKKVKKTIPAWATLSASQLARAQKQTQMAASSRPKMDAILIEAIKACYQKGGASVVAIRKFIISRYPSLDLERRGYLLKQALKRELERGVIRQVKGKGASGSFVVAPNSGKTGSKAKDRKRSLSASSAEQHVKLEDILPLAFTRLCEPKEASYSLIKKYVSQYYPKLKVDIRPQLLKNALQRAVEKGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEDAILSAIAAMNEPKTCSTTALKKYVLEKHPGTNSSFQVHLLKRTLQKCEKYGWMEQISGKGFSGTFQLCFPYYPSPGILFPEKQKEDKEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNQ4</accession>
    <name>A0A6I9YNQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106551909</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925549.1">
        <property type="nucleotide sequence ID" value="XM_014070074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNQ4"/>
    <dbReference type="GeneID" id="106551909"/>
    <dbReference type="KEGG" id="tsr:106551909"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="577"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="614"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="666"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="701"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="727"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="507"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925549.1"/>
        </source>
    </evidence>
    <sequence length="772" mass="88362" checksum="4C43888C99200232" modified="2020-10-07" version="1">MVFAVKKINEDVQLLPNITLGFRIYDKSAEYHAKMELLSSQNRFVPNYKCGTLNKLISVVGTVDPGIGSDMADILEAYKIPQFPTDCFQYESHFYYPMVPGEQFYYRGIIKLFLYFRWTWVGILGAKFDQLIISEFPLHGICIAFLEKFRPVYIDNFFDNLKWLVEMFHLLVKSKANAIILNENNLLNVRWLLRLPEMEIVSVKPKGKVWIVASQMGLASFFYQKSWDIQVLHGALTFAAHSSEITGFQHFLQTRNHFSSKGDGFVKNVWEQAFGCAVPNPSENEETEGLCTGKEKLESLPGAFFEMKMTSHSYSIYNAVYAVAHAFHAMMCGQQHRRKLKGRKLNLQDLQPWQLHFFLKRISFNNSVGDEISFDENKELITELDLENWVIFPNQSLHRVKVGKLDPWAAEDQKFTIHEEDITWPSIFNQTLPISVCTESCHPGYFRKKQEAKPFCCYDCLQCSKEKMSSQKDMDDCLKCPEDQYPNKNQTSCIPKKICFLSFEEPMGIGLAIMVLFFSLCTIVVFTTFLKYHHTPIVKANNRNLTYILLISLLLCFLCSLQFLVVPGNIICVLRQISFGIIFSVAVSSVLAKTITVVLAFMATKPGSRMRNWVGRRVALSIVLSGSLLQAGICTVWLFTFPPFPDMDTHSEMEQIILMCNEGSSTMFYCVLGYMGFLAAASFTVAFFSRNLPSSFNEAKCLTFSMLIFCSVWLSFVPSYLSTKGKYMVAVEIFSILASSAGLLICIYFPKCYIIIFKPELNSREQLIKRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPT7</accession>
    <name>A0A6I9YPT7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Melanoma-associated antigen G1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106552707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926523.1">
        <property type="nucleotide sequence ID" value="XM_014071048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPT7"/>
    <dbReference type="GeneID" id="106552707"/>
    <dbReference type="KEGG" id="tsr:106552707"/>
    <dbReference type="OrthoDB" id="205198at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1210:FF:000001">
        <property type="entry name" value="melanoma-associated antigen D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1200">
        <property type="entry name" value="MAGE homology domain, winged helix WH1 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037445">
        <property type="entry name" value="MAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041898">
        <property type="entry name" value="MAGE_WH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736:SF14">
        <property type="entry name" value="NSE3 HOMOLOG, SMC5-SMC6 COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50838">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="52"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926523.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="30148" checksum="530154866C59683D" modified="2020-10-07" version="1">MSQQHGHSGRNSRGAGSSKVRHMEVDEEFTLSQTQTLTQAQRNLEQHSPAEVEQKVNELVQFLLIKDQKKIPIKRTDILKYVIKDCKDIFPEILRQANQTLQRVFGLGVVEIDPKYHSYILISKFPPLEAENVNDDENTPKMGLLIVILSLIFMKGNVIKESAVWEMLRRLHVDCSGGKHKVFGDVKKLVTEEFVRQKYLEYTRLPHTDPPEFEFRWGPRAFKETSKKQILQFVAKIQNQNPKSWMSQYNEAEAEANAVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQA4</accession>
    <name>A0A6I9YQA4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 151A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TMEM151A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM151 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926094.1">
        <property type="nucleotide sequence ID" value="XM_014070619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQA4"/>
    <dbReference type="GeneID" id="106552368"/>
    <dbReference type="KEGG" id="tsr:106552368"/>
    <dbReference type="CTD" id="256472"/>
    <dbReference type="OrthoDB" id="190434at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026767">
        <property type="entry name" value="Tmem151"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31893">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 151 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31893:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 151A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14857">
        <property type="entry name" value="TMEM151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013926094.1"/>
        </source>
    </evidence>
    <sequence length="484" mass="54489" checksum="F7DB94EFA0032603" modified="2020-10-07" version="1">MPGVGAEDGGQQPGAGELPTLTPAGGPPREEQRPLKQSLSSSLCRESHWKCLVLTLLMFGCFGVMAWCRLSTVTRLNYDGSYRGASLVYHDSPCSNGYIYIPLAFLGMLYVVYLVECWHRFAKGQMQFRVDTASVYEKVQRMQQATPCIWWKAVSYHYIRRTRQVTRYRNGDAYTTTQVYHERVNTHVSETEFDYSGHGVKDVSKELLGLSDYPVTKLRFTKCFSFASGEAEAAYLTQRARFFGENEGLDDYMEAREGMHLKNVDFKEHMLVFPDPGRPPWYTRRSVFWLASFLLLSWPLRVLSEYRTANVHYHVEKLFGADDGPSPLDAAGTYGRRISRVNTVDMTELEWHIRSNQQLVPSYSEAVLMDTTTVAGAAYLRSCQRCRRTVSSNSLPSRGTRALYARLPFSCSRFSLSGARRCGGILRSLSGSQVGSNIETEPLESPPCYQDALYFPVLIVHGGEGCERNGHRPGLAGRGQGTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRK6</accession>
    <name>A0A6I9YRK6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dynactin subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DCTN2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynactin subunit 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926564.1">
        <property type="nucleotide sequence ID" value="XM_014071089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRK6"/>
    <dbReference type="GeneID" id="106552743"/>
    <dbReference type="KEGG" id="tsr:106552743"/>
    <dbReference type="CTD" id="10540"/>
    <dbReference type="OrthoDB" id="4977at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15346">
        <property type="entry name" value="DYNACTIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0243">Dynein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926564.1"/>
        </source>
    </evidence>
    <sequence length="405" mass="44639" checksum="196AB8D222EADF69" modified="2020-10-07" version="1">KYADLPGIARNEPDVYETSDLPEDDQAEFDAFAQELEELTSTSVEHLIVNPNAAYDKFKDKRVGTKGLDFSDRIGKSKRTGYESGDYEILGEGLHVKETPQQKYQRLLHEVQELTTEVEKIKNSVKESSAEEKLTPVTLAKQVAGLKQQLVSTHLEKLLGPDAAINLTDPDGALAKRLLLQLEVTKTAKSVSAGGKGAAKVTAGPENTVTYELHSRPEQDKFSQAAKIAELEKRLAELEASVRCEQDVQNPLSVGLQGASLMETVELLQAKVNSLDVATLDQVEARLQSVLGKVNEIAKHKATVEDTDTQSKVHQLYALVQEWSPLASTLPEVVQRLVAVRQLHEQAMQFGQLLTHLDTTQQMIANSLKDNVTLLTQVQKAMKENLAAVEDNFANINSRVQKLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRZ4</accession>
    <name>A0A6I9YRZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP-binding protein RAD-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926390.1">
        <property type="nucleotide sequence ID" value="XM_014070915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRZ4"/>
    <dbReference type="GeneID" id="106552599"/>
    <dbReference type="KEGG" id="tsr:106552599"/>
    <dbReference type="OrthoDB" id="5239715at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051641">
        <property type="entry name" value="RGK_GTP-binding_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775:SF3">
        <property type="entry name" value="GTP-BINDING PROTEIN RAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775">
        <property type="entry name" value="RAD, GEM/KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926390.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17051" checksum="950869E25F2E65E2" modified="2020-10-07" version="1">MTLNRDDKLRYLDKRRGSMPFAAHHPQQPLHRRSMPVDERDLQSSLPPSPRGQLSGLMRCTSYNPGEEEEEETQRPGWNSDSSDSVISSGSDSEGGLYKVMLLGEHGVGKTSLARIFGGVEDGPDAEEAGNTYDRSIIVDGEEASLVVFDIWEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT95</accession>
    <name>A0A6I9YT95_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106552992</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926880.1">
        <property type="nucleotide sequence ID" value="XM_014071405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT95"/>
    <dbReference type="GeneID" id="106552992"/>
    <dbReference type="KEGG" id="tsr:106552992"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="207"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926880.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="54059" checksum="25DFB8FAFF156049" modified="2020-10-07" version="1">MVQPAQLFFVFTIFEWVVEQILSGPPPKNCLIWLYLFSISLRIIRVHKPLHYDKLHFFLKRVSFNNSAGDKISFDKYRKLITGLEIENWVTFPNQSLHQVKVGKLDPWAVEDKMFTIHEEAIKWPSTFNQTIPVSVCTESCLPGYFKKKQEEKPFCCYDCLLCPKEKISSQKDMHDCSKCPEDQYPNKEQDSCIAKKISFLSYEEPMGIGLFTLALFFTLCTTVILRIFLKYHNTPIVKANNRNLTYLLLISLILCFLCSLQFLVMPSNIICLFRQISFGIIFSVAVSSVLAKTITVVLAFMATKPGSRMRNWVGRGLAIPIVLSGSLIQTGTCMVWLFTFPPFPDIDTHSKMEEIILQCNEGSATMFYCVLGYMGFLATASFTVAFFSRNLPSSFNEAKCLTFSMLIFCSVWLSFVPSYLSTKGKYMVAVEVFSILSSSAGLLVCVYFPKCYIIIFRPELNNREELIKRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUK5</accession>
    <name>A0A6I9YUK5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein 544-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553374</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927325.1">
        <property type="nucleotide sequence ID" value="XM_014071850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUK5"/>
    <dbReference type="GeneID" id="106553374"/>
    <dbReference type="KEGG" id="tsr:106553374"/>
    <dbReference type="OrthoDB" id="5803771at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001009">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="629"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="714"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="322"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="416"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927325.1"/>
        </source>
    </evidence>
    <sequence length="772" mass="86262" checksum="72E924511743920E" modified="2020-10-07" version="1">MLPHTYTLKGLRMVANSCVGEGERHPNGKMEPLEERLAHEGWRHQHFHNVADRGYKRQETAAHSGREIGRALEEDPTRCREKWKQLRDKFARLKKRMKTKSRDPGPPAHSLMLSWLEGFVKLWETQVNVNGAATEPEDATPGPLEKDSLDSTLNMEDISFTSHLASKHSGVFSLHSSDDDEDEAPVSQSRKRRKKMGKDEILDALVTLRREQLQTTMMTLLNPDNGRRNDEYTTFALAVADSLRKLPPEHVEATKSQLFIVLADAHSQVTKPSGTGGTDPTELLQTTVELGSFSTDPGMTARPLSQQSFSISVQELQPEINAEDQEAEAADKPEQKAEDGEGNSHPLSQHPTESELRTSCSLGQSSATEPEQECRYSLETVAFSPPPLEFRESPNCLEAEAEEEKMLETQISFSGEMSPSFADPVKENRELGLEEESLNGETQGSQDEKVRRPLADINSSTPIQEPLGGVKIQHGKETTQEGDFLGKGPGSGSENSTLESKDGFQLEKPKEINPDGKLLERALGTLIQGPNGQESLEIEQDMVSLPEVKSDLALFCDEVSANGPAKTLQGREASFDLSGNLQPRADPSESQNAPMENERIYKCSYCGKCFEESDSLVTHERAHIAEKIYRCSQCEKHFSHRLDLLTHQRNHQREKPHQCERDCAKCHRQRTCPRATHRARPGEQACQCPVCGERFTWKSNLIRHRRIHTGEKPYRCAECGKSYTRKNALDRHKRIHVGEKAHEVGAPSMGQASVLVLLGKLPVRRTSACREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV84</accession>
    <name>A0A6I9YV84_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spermatogenesis-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SPATA1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928518.1">
        <property type="nucleotide sequence ID" value="XM_014073043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV84"/>
    <dbReference type="GeneID" id="106554395"/>
    <dbReference type="KEGG" id="tsr:106554395"/>
    <dbReference type="CTD" id="100505741"/>
    <dbReference type="OrthoDB" id="9901850at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039062">
        <property type="entry name" value="SPAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031478">
        <property type="entry name" value="SPATA1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14421">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14421:SF3">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15743">
        <property type="entry name" value="SPATA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 1 C-terminal" evidence="3">
        <location>
            <begin position="416"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928518.1"/>
        </source>
    </evidence>
    <sequence length="467" mass="53393" checksum="18AD14DFA0FC2A58" modified="2020-10-07" version="1">MTSRNMLSNQNRLRTSELLELHVFYVPEEVWNFKLNTVSIEVISKLFSAGFIRVSPHMTLKTLRENLGEYLGEDAVIDKYVFLKCIGEKLVVVKAKQEMELELKSFAPPYAFHPELYLLPGIENIYSSLSPTPEKHQNNPEYVLTSVSYERPHSLISPNPDIYPNSTMLDSSFKNNLSQTWEKAGLGERNEKEIFPVLHKKQSSEQEKNQIIQGKSSSQQRKGKISCNIDKTESAPSKQHPTKKSAAVKMYQKNTDSSPPEMHNIPGKDEKYRKTVASPKIDQNHEKEENNQFPLSHISQKTKEEVSLMLDTNAENGGNLTGIGNGRHTTTADSGISKSLEDRDITYSPKKRSQQHPGMTKTAAVQNNQADGNNLNDSAYPSHYLSPPTPPLLAVSVNRAQLPHKDFPTEGNQLHKQLKHIKIERRHLENTRAELVKKVKNLIEQHKLRRCRVRDYWKKKYFEMKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YW05</accession>
    <name>A0A6I9YW05_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metalloproteinase inhibitor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Tissue inhibitor of metalloproteinases 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106554122</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Interacts with MMP1, MMP3, MMP10 and MMP13, but has only very low affinity for MMP14. Interacts with CD63; identified in a complex with CD63 and ITGB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928204.1">
        <property type="nucleotide sequence ID" value="XM_014072729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YW05"/>
    <dbReference type="GeneID" id="106554122"/>
    <dbReference type="KEGG" id="tsr:106554122"/>
    <dbReference type="OrthoDB" id="6041373at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF20">
        <property type="entry name" value="METALLOPROTEINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="6 20" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 20" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="NTR" evidence="18">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="113"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="118"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013928204.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20680" checksum="E580EA7F4A4FD991" modified="2020-10-07" version="1">MVIRGKFVSVSKQHVNVSVGEPVWWVRHEIKTTKVYKGPEEVQDVRFLYTPAMESLCGYEHKGPLKGEEYVIAGMMDGNRVMITACSFIQPWSQLSLAQKRGLSLEYSKGCNCTVMSCLSMPCFISSDNQCLWTDGIMSRIWDGLQTKRLACLPRSESSNFCTWQSLTAQGPGSLRKSRLLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWB0</accession>
    <name>A0A6I9YWB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="2">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106554197</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013928281.1">
        <property type="nucleotide sequence ID" value="XM_014072806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWB0"/>
    <dbReference type="GeneID" id="106554197"/>
    <dbReference type="KEGG" id="tsr:106554197"/>
    <dbReference type="OrthoDB" id="1845775at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001123">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1510.10">
        <property type="entry name" value="Domain 2, N(10)-formyltetrahydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928281.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="41899" checksum="193F74A06543784C" modified="2020-10-07" version="1">MSISDLACFFSSLQAHFDIAVASEIMAVLALTNGLEDMRERLGKMVVASSKKGVPITTEDLGVSGALAVLMKDAIKPNLMQTLEGNPVFVHAGPFANIAHGNSSVLADKIALKTVGKDGFVVTEAGFGADIGMEKFFDIKCRYSGLQPHVVVLVATIRALKMHGGGPTVMAGVPLPKEYIVENIPLLEKGCSNLRKQIENARIFGVPVVVAVNAFKSDTEAELQLVIRMAKEAGALDAVKCTHWADGGKGAVALAEAVQRASQAPSHFQFLYNVELPVVEKIRIIAQKIYGANDIELLPEAQFKIIQYTKQGFGNLPICMAKTHLSLSHDPELKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPVTGQVNGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXK2</accession>
    <name>A0A6I9YXK2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Polycomb group protein ASXL3 isoform X4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ASXL3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Asx family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929388.1">
        <property type="nucleotide sequence ID" value="XM_014073913.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555136"/>
    <dbReference type="CTD" id="80816"/>
    <dbReference type="OrthoDB" id="9348951at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035517">
        <property type="term" value="C:PR-DUB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042975">
        <property type="term" value="F:peroxisome proliferator activated receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026905">
        <property type="entry name" value="ASX-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024811">
        <property type="entry name" value="ASX/ASX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028020">
        <property type="entry name" value="ASX_DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007759">
        <property type="entry name" value="Asxl_HARE-HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13578">
        <property type="entry name" value="ADDITIONAL SEX COMBS LIKE PROTEIN ASXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13578:SF18">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN ASXL3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13919">
        <property type="entry name" value="ASXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05066">
        <property type="entry name" value="HARE-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13922">
        <property type="entry name" value="PHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51913">
        <property type="entry name" value="HTH_HARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HTH HARE-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="12">
        <location>
            <begin position="228"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="119"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="345"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="506"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="580"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="893"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1739"/>
            <end position="1764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1948"/>
            <end position="1998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="510"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="527"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="580"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="599"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="898"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="910"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="958"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="999"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1739"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1955"/>
            <end position="1964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1967"/>
            <end position="1977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013929388.1"/>
        </source>
    </evidence>
    <sequence length="2174" mass="236338" checksum="F9AC2A1C69026650" modified="2020-10-07" version="1">MTAKQILEVIQKEGLKETSGTSPLACLNAMLHTNTRVGDGTFFKIPGKAGLYALKKEDTTCPADGTLDLSCESELDSNEMAETNNSNGEENGVCPKQAPEELSSIRDSSLTNAPMQSKLVSSFQQHTKKALKQALRQQQKRRNGVSMMVNKTVPRVVLTPLKVSDEQSDSPSGSESKNGEADNSDKEMKHGPKSPTGKQMSQHLKRLKKSGLGHLKWTKAEDIDIETPGSILVNTNLRALINKHTFLSLPQHFQQYLLLLLPEVDRQMGSDGVLRLSSSALNNEFFAYAAQGWKQRLAEGEFTPEMQLRIRQEIEKEKKTEPWKEKFFERFYGEKLGMSVEESRKLTSVQNNEDDNKSLSGSSDLPGPSREPSFDDHEEKSNESPLLPERDFCQALCNMEHVPSKDLMAETEDILIPEESIIQEEIAEEVETSICECQEENQKEHMNSEQLVSPVGTNEETDPLPLTGNSESCVVMNDVLSPLSHVEIKEEQRLEYPKEEMSVITDQIESSLSPSQSASSSNSVSNVEEKDLESIKELSPDGKYSPVLNGSLFTGGGVAVHMELQSDPDELSSENACISETSFSAESPEEPSASITSPGGDTQSTSEEPSTPVSLETVCSSDMSCTENNETDTQQKSVDDNLNTSLMSEISPLPASPVISEASLLSNLPLTSETSPVSNLPLPSEISPMSDLPLTSETSSVSSVLLASETSHSNSLLLPSEASLLSNSPVSERFIQQRKTLCLSEESLSPLKEETCSIHNIPQEDDIPSQQNSLQGVPDAMKAVIAEPSPAEGSQSRNHTNQTCRSQTEIENCFISSLAESSSPEVIKMRNHGNHQPSENKSAISQLEVSVLTKVAECKNIESLPSKSHDKLYTSTSSLEMGKNKSHKLQGTIQNRFESSYSSKSSEVIKSPEIRNESRDLDIPKRKTAEHHSLGICKEKRARIEDDQAHRNSSTNSSEREPPPREEPRVPPLKIQLSKIGPPFIIKSQPVSKPEPKATPSTSVSSGRNTGARTLADIKARAQQARAQREAAAAAAVAAAASIVSGGLGSPCEGGKTRTLAHIKEQTKAKLFAKHQARAHLLQSNKESKSQQALKEAPSVVEISVASETKIEGSTGVIIVNPNCRSPSNKSAHLREATALLQHSLNATSLPETSTDITVQNSDESISVPHFCEKMLSSTSPESISVLYKKNSVPDAVCSTVKSGTIKELSFAGAVDKPSVLMSVESTDMKTSNITMLKSIQRADTPFVSIVPKCHENSAPPATANTTILSDSLEKKILPRPSSNATSAISSQYTTVPTCIASTLSDHLSGSSVLIPPGGSISRYSSDKISLTGCSDPSALSNSISVRAALHGNEALSVGDSIGRTSISIFASNMMTVNSYENSTKMSADGIERNSGMRSRLDMPGKPSVGYIQAPANRSIPCKVIVDHTMTTMNSSLSLGLSMESTDSSVEAQNRPIRIETALQNITCPQVSVISRPELLNNENLEPSSGFNNGPTQQDGKHLQAACTAIREMPLGPQDKQLDVVPPTQGFSEQFQEPLTFKREAESICTNRYNPNSRICWNDNEVMNTEQSLVSHLNPTKHKEYSENNCLKNVKTEPSGLVHLHEMHTRNIVTSIALPVKSERNESDKCFRMDMEDFVRPEIPVQTAEIAPSSQPVQSSKRSVTDSIEPSLALTTETLKRVTNAGTSSCRLSSVEANNPLVTQLLQGNLPLEKVLPQPRLGAKLEISRLPLPLQTTSRCKSVTSERNTVENLSSSPNADGRDFAAINIPPIQIRKRENHPKKRVARTAGEIKCEPGKPSVDSDIKVTPCIITSSMNQLGHGQLFKQEWPSKHTIQSRIANSPEIKQQKRPLPSCSFQKSLFSVEKNGSFHTETSTSHQPHFYQMPMAARGPVTTATLMQATLKAPSGCSSFAFSRNLEHKALGEANMSTAPHQLRLATVFPPNVQIKESDDIPSASQTLQNKSLGHPPPPPPPPNAEAPSDHKQPGVTMETTKRLSWPQPANVCSNIKSEPISLEEGLSSSCELGMKQPSYDQNEVKEQLKAFALKNADFPSYLLSESQKPFAPLTAPKIQAQQQRGNYPTIHFGSTSFKRATSAIEKSIGILGSSSTTTTSLPIQNAQIPVQKFADNSSADELELKCSCRLKAMIVCKGCGAFCHDDCIGPSKLCVACLVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXS1</accession>
    <name>A0A6I9YXS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ARCN1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11 12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11 12">Cytoplasmic vesicle</location>
            <location evidence="11 12">COPI-coated vesicle membrane</location>
            <topology evidence="11 12">Peripheral membrane protein</topology>
            <orientation evidence="11 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11 12">Golgi apparatus membrane</location>
            <topology evidence="11 12">Peripheral membrane protein</topology>
            <orientation evidence="11 12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929458.1">
        <property type="nucleotide sequence ID" value="XM_014073983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXS1"/>
    <dbReference type="GeneID" id="106555199"/>
    <dbReference type="KEGG" id="tsr:106555199"/>
    <dbReference type="CTD" id="372"/>
    <dbReference type="OrthoDB" id="10266042at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051645">
        <property type="term" value="P:Golgi localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09254">
        <property type="entry name" value="AP_delta-COPI_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14830">
        <property type="entry name" value="Delta_COP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000007">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000011">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000003">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027059">
        <property type="entry name" value="Coatomer_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121:SF0">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="14">
        <location>
            <begin position="271"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="136"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013929458.1"/>
        </source>
    </evidence>
    <sequence length="511" mass="57109" checksum="869B5D792DDF5E9F" modified="2020-10-07" version="1">MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLMNTGKQHTFVETESVRYVYQPLEKLYMVLITTKNSNILEDLETLRLFSRVIPEYCRALEENEIAEHCFDLIFAFDEIVALGYRENVNLAQIRTFTEMDSHEEKVFRAVRETQELEAKAEMRRKAKELQQARRDAERRGTKAPGFGGFGNSAVSGGTLAAMITETIMESEKPKITPAPTRPCGSNKALKLGTKGKEVDNFVDKLKSEGENIVASSVGKRSSNAAAVLASPINMESVHMKIEEKISLTCGRDGGLQNMELHGMIMLRILDEKFARICIHIENEDKKGVQLQTHPNVDKKLFTSESVIGLKNPDKSFPINSDVGVLKWRLQTTEESLIPLTINCWPSESGNNCDVNIEYELQEENLELNDVVIAIPLPPGVGAPVIGEINGEYRHDSRKNLLEWCLPVVDAKNKSGSLEFSIAGRPDDFFPVNVSFVSKKNFCNIQVTKVTQVDENSPVKFSTETTFLVDKYEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY27</accession>
    <name>A0A6I9YY27_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ADAMTS-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106554742</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928949.1">
        <property type="nucleotide sequence ID" value="XM_014073474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY27"/>
    <dbReference type="GeneID" id="106554742"/>
    <dbReference type="KEGG" id="tsr:106554742"/>
    <dbReference type="OrthoDB" id="5948003at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928949.1"/>
        </source>
    </evidence>
    <sequence length="247" mass="27621" checksum="2FB952335B3853BB" modified="2020-10-07" version="1">MASAIGTKVTVAAGDTLRIGCPVYPSTNNTIRWFARNQPVEETSGFRHKTLVGGRILEFIVASDQFAGQYKCWASSHIKPLSVWVNVQKEEYHWKLAEQSPCSTACGNLGTRFWKFLCVNTKDQNVNASLCKDHQKPVVNNPQSCEIQDCPARWATTAWSECSASCGHGFHRRRITCKQVKANRRVLTLPPGACADQERPLEIKPCVGRFCAEWMVHPWGQVINSMMNESPLPLAVHQPLLRSLKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZW4</accession>
    <name>A0A6I9YZW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PCYT2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013929411.1">
        <property type="nucleotide sequence ID" value="XM_014073936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZW4"/>
    <dbReference type="GeneID" id="106555158"/>
    <dbReference type="KEGG" id="tsr:106555158"/>
    <dbReference type="CTD" id="5833"/>
    <dbReference type="OrthoDB" id="40021at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02173">
        <property type="entry name" value="ECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000108">
        <property type="entry name" value="Ethanolamine-phosphate cytidylyltransferase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026968886" description="ethanolamine-phosphate cytidylyltransferase" evidence="12">
        <location>
            <begin position="28"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="17"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="206"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929411.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="43471" checksum="12B0C3A9C59EAC08" modified="2020-10-07" version="1">MPLQKFLCYFWFCLFLCSYDMVHYGHSNQLRQARAMGDYLIVGVHTDDEISKHKGPPVFTQEERYKMVKAIKWVDEIVEGAPYVTTLETLDKYNCDFCVHGDDITLTADGKDTYEEVKKAGRYRECKRTQGVSTTDLVGRMLLMTKAHHSNIAEETDYRKHADNLGKCPKGHSPWTGVSQFLQTSQKIIQFASGKEPQPGDTIIYVAGAFDLFHVGHVDFLERVHQIAKHPYIIAGLHFDQEVNHYKGKNYPIMNVHERTLSVLACRYVSEVVIGAPYSVTADLLDHFKVDLVCHGMTEVVPDKDGSNPYEEPRRRGIFQLVDSGNNLTTDLIVKRIIKNRLEFEARNQKKEAKELAVLEVMKRKEEAAQQEIGLRNQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZZ0</accession>
    <name>A0A6I9YZZ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Calcium-regulated heat stable protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CARHSP1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929872.1">
        <property type="nucleotide sequence ID" value="XM_014074397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZZ0"/>
    <dbReference type="GeneID" id="106555527"/>
    <dbReference type="KEGG" id="tsr:106555527"/>
    <dbReference type="CTD" id="23589"/>
    <dbReference type="OrthoDB" id="448492at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000086">
        <property type="entry name" value="Cold shock domain-containing protein C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052069">
        <property type="entry name" value="Ca-reg_mRNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12962">
        <property type="entry name" value="CALCIUM-REGULATED HEAT STABLE PROTEIN CRHSP-24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12962:SF3">
        <property type="entry name" value="CALCIUM-REGULATED HEAT-STABLE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="62"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929872.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16062" checksum="4B533F589C291BD4" modified="2020-10-07" version="1">MSSESTSAHQRSPPPRALHLPEGRRTRDRSPSPMRGYLIPSPLPTRRNRTFSATTRAAEGPTYSGVCKCFCRSKGHGFITPADGGPEIFVHISDIEGEYVPVAGDEVTYKVCTIPPKNEKLQAVEVNITHLAPGTKHETWSGHVVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z093</accession>
    <name>A0A6I9Z093_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 91-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106555977</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930403.1">
        <property type="nucleotide sequence ID" value="XM_014074928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z093"/>
    <dbReference type="GeneID" id="106555977"/>
    <dbReference type="KEGG" id="tsr:106555977"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002737">
        <property type="entry name" value="AGAP008430-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003000">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000258">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000116">
        <property type="entry name" value="Zinc finger protein 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000149">
        <property type="entry name" value="Zinc finger protein 569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000990">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF178">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="25"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="586"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="614"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="642"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="670"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013930403.1"/>
        </source>
    </evidence>
    <sequence length="694" mass="79399" checksum="D8F13BC0A8E6F784" modified="2020-10-07" version="1">MECGKVFGQHRNLTIHKRIHTGEMPYKCMECGKSFCENAFFVRHKRIHQGEKPNKCLEYGKGFTNNSQLTSHKRIHTGEKSHTCKECGKSFRHVVSLSYHNKIHTGEKPYKCMECGKSFVNSSHLISHKRVHTGKKRCKCMECGNGFSDSCSLTFHKRIHTGEKLYKCLECGKTFTHSSQLTPHKRTHTGEKPYKCKECDKTFTHSSQLITHKRIHTGERPYKCKECGKSFCENASLMKHEKIHAGEKPYKCMECGKSFTQSSNLTSHKRIHTGEKPYKCRECGKSYSHSVSLTYHNRIHAGEKPYKCLDCGKTFTQSSQLTSHKRIHTGEKPYQCMECGKGLYDNVSLMRHKRIHTGEKPYKCRECGKGFTQQGNLNSHERMHRGEKPYKCRECGKSFSHSGSLTYHNRTHTGEKPYKCLVCGMGFTVSSHLTSHKRIHTGEKPYNCRQCGKNFSENSSLMRHKRIHTGEKPYKCVECGKHFTHSGSLTSHKRIHTGEKPYKCMECGKSFTTSSDLTSHKRIHTGEKPYKCMECGKSFTTSSVLTSHKRIHTGEKPYKCMECGKSFTISSDLISHKRIHTGEKPYKCMECGKSFTISSNLTSHKRIHTGEKPYKCMECGKSFTISSDLISHKRIHTGEKPYKCMECGKSFTISSNLTSHKRIHTGEKPYKCMECGKSFTISSDLISHKRIHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0A8</accession>
    <name>A0A6I9Z0A8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC106555048</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013929290.1">
        <property type="nucleotide sequence ID" value="XM_014073815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0A8"/>
    <dbReference type="GeneID" id="106555048"/>
    <dbReference type="KEGG" id="tsr:106555048"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10475">
        <property type="entry name" value="EphR_LBD_B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05065">
        <property type="entry name" value="PTKc_EphR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
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        <property type="entry name" value="ephrin a2 ectodomain"/>
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    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
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    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
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        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
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        <property type="entry name" value="Ephrin_rcpt_TKs"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
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    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
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    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
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    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
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    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF15">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR 6"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM/Pointed domain"/>
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    </dbReference>
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        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
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    </dbReference>
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        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 22" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027031144" description="receptor protein-tyrosine kinase" evidence="22">
        <location>
            <begin position="31"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="560"/>
            <end position="584"/>
        </location>
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    <feature type="domain" description="Eph LBD" evidence="26">
        <location>
            <begin position="32"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="343"/>
            <end position="451"/>
        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="452"/>
            <end position="547"/>
        </location>
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        <location>
            <begin position="636"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="24">
        <location>
            <begin position="928"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="905"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="909"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="761"/>
        </location>
    </feature>
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        <location>
            <begin position="642"/>
            <end position="650"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="17 19">
        <location>
            <position position="668"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <begin position="74"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00051243"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
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    <evidence type="ECO:0000256" key="18">
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="1001" mass="111566" checksum="891A537A142E16E3" modified="2020-10-07" version="1">MDPNIIRRMKEHAVIMVWLWIMCLLGLASSLEEILLDTTGETSEIGWTAHPPEGWDEVSVLDDRKHLIRTFEVCNVAEPGQNNWLRTHFIERRGAHRIHIRLRFSVRDCASMRTVASSCKETFTLYYHESETDLATAVYPEWHEGPWTKVDTIAADESFSQVDSSGKVVKMNVKVRSFGPLTKRGFYLAFQDSGACMSLVAVQVFFYKCPAVVKGFASFQETFAGGERTSLVEAPGTCVADAEEASSTGSSGVKLHCNGEGEWMVAIGRCACRAGYESMDSETNCKACPKGTFKASAGDAPCSPCPTHSYASIPGSSECACQTHYYRASSDISDAPCTSIPSAPRDLSYEIIGSNVLLTWRLPKDLGGRKDVFFNVICKVCPNGSPGPCVRCGDSVQFEPRQVGLTESRVQVSNLLARVQYTFEIQAVNLVTELSPNSPHYAAINVSTSQSVPSAVPMMHQVSRTPSSITLSWPQPDQPNGIILDYQLRYFDKSEDEDTSFTLTSETNMATISSLSPGKIYAFQVRARTAVGYGPYSGKMYFQTLVGGERSEMVQDRLPLIVGSALGGLAFMVIAAIAILAIVFKSKRRETPYTDRLQQYISTRGLGVKYYIDPSTYEDPNEAIREFAKEIDVSFVKIEEVIGSGEFGEVCFGRLKPPGKREYMVAIKTLKAGYTDEQRREFLSEASIMGQFEHPNVIRLEGVVTKSRPVMIVTEFMENGSLDSFLRQKEGQFSMLQLVGMLRGIAAGMRYLSDMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDASNPTYTGALGGKIPIRWTAPEAIQYRKFTSSSDVWSYGIVMWEVMSYGERPYWDMSNQDVINAIDQDYRLPPPPDCPTVLHLLMLDCWQKERVQRPKFEQIVNALDKMIRKPSALKATGTGNSRPSQHLLSNSPPDFPSLSNAHEWLDAIKMGRYKDNFDQAGLTTFDVISRMTLEDIQRIGISLVGHQKKILNSIQLMRVHLNQLEPVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0C4</accession>
    <name>A0A6I9Z0C4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106555061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013929305.1">
        <property type="nucleotide sequence ID" value="XM_014073830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0C4"/>
    <dbReference type="GeneID" id="106555061"/>
    <dbReference type="KEGG" id="tsr:106555061"/>
    <dbReference type="OrthoDB" id="5779068at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000423">
        <property type="entry name" value="Slingshot protein phosphatase 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043587">
        <property type="entry name" value="Phosphatase_SSH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043588">
        <property type="entry name" value="SSH-N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864:SF5">
        <property type="entry name" value="PROTEIN PHOSPHATASE SLINGSHOT HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864">
        <property type="entry name" value="SLINGSHOT PROTEIN PHOSPHATASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23040">
        <property type="entry name" value="PH_SSH1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="5">
        <location>
            <begin position="92"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929305.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="28253" checksum="746D8A2D567F500D" modified="2020-10-07" version="1">GFSVSTAGKTHIFKPVSVQAMWSALQILHKACEMARRHNYFPGGMALVWATYYESCIASEQSCLNEWNAMQDLESPRPDSPALYMDKPTERERAERLIKAQLRSIMMSKDLENVTSKEIRNQLEKQMSCNLNEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELRGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKARCPSRSHCQHRLHSVIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z272</accession>
    <name>A0A6I9Z272_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106555748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930121.1">
        <property type="nucleotide sequence ID" value="XM_014074646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z272"/>
    <dbReference type="GeneID" id="106555748"/>
    <dbReference type="KEGG" id="tsr:106555748"/>
    <dbReference type="OrthoDB" id="338614at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="1">
        <location>
            <begin position="24"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930121.1"/>
        </source>
    </evidence>
    <sequence length="269" mass="29372" checksum="79AA77F98E5AD774" modified="2020-10-07" version="1">MSIPMPGHPVNFSETTLQQAQKDVAQLEKLIDGHDVIFLLMDTRESRWLPAVVSASKRKLVINAALGFDTFVVMRHGLKKPKNPEIGDSPCNNSSGSVDLLGSSLFSNIPGYKLGCYFCNDVVAPGDSTKDRTLDQQCTVSRPGLAMIAGALAVELMVSILQHPEGGYAVASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSPKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDISDDETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3L5</accession>
    <name>A0A6I9Z3L5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CDK16</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013930561.1">
        <property type="nucleotide sequence ID" value="XM_014075086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3L5"/>
    <dbReference type="GeneID" id="106556115"/>
    <dbReference type="KEGG" id="tsr:106556115"/>
    <dbReference type="CTD" id="5127"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07873">
        <property type="entry name" value="STKc_PCTAIRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000061">
        <property type="entry name" value="Putative cyclin-dependent kinase 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF174">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="184"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013930561.1"/>
        </source>
    </evidence>
    <sequence length="527" mass="59924" checksum="0BB0110509374693" modified="2020-10-07" version="1">MDRMKKIKRQLSMTLRGSRTVEKNLNEPINIEENNSSDNGPTVKSVHPSTSRSLHAFLHHYAGSFRRPRLAVSRSLSSHLGRTSRLEIVHEDLKMGSDGESDQASGTSSDEVQSPVRVRMRNNPARKISTEDINKRLSLPADIRLPEGYLEKLTLNSPLFDKPLSRRLRRVSLSEIGFGKLETYVKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNVINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTEYSTQIDMWGVGCIFYEMSTGRPLFPGSTVEEQLHFIFRILGTPNEETWPGILSNEEFRAYNYPKYRSEALINHAPRLDNDGVDLLGKLLQFEGRKRISADEAMRHLFFHSLGERVLKLPDTTSIFALKEIQLQKETGLRSASLPESGEKWLSSNSVNRTPSAFPLALSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3R3</accession>
    <name>A0A6I9Z3R3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930869.1">
        <property type="nucleotide sequence ID" value="XM_014075394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3R3"/>
    <dbReference type="GeneID" id="106556397"/>
    <dbReference type="KEGG" id="tsr:106556397"/>
    <dbReference type="OrthoDB" id="14970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051750">
        <property type="term" value="F:delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045002">
        <property type="entry name" value="Ech1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149:SF1">
        <property type="entry name" value="DELTA(3,5)-DELTA(2,4)-DIENOYL-COA ISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930869.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15587" checksum="7DFF2DEF20006647" modified="2020-10-07" version="1">MASKPPQQQQQQPPPNSLPAYETLKLERVREKVLHVELNRPEKRNAMNVAFWREMVECFNKIAQDSECHAVVISGAGKMFTSGKKDGGVTSERALLLRSGKSSKELPGVHLLGSFSWKGFLSARRMNPSLPHHPVRAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3V0</accession>
    <name>A0A6I9Z3V0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Melanin-concentrating hormone receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">G-protein coupled receptor 24</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">MCH-1R</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106556433</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with NCDN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930904.1">
        <property type="nucleotide sequence ID" value="XM_014075429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3V0"/>
    <dbReference type="GeneID" id="106556433"/>
    <dbReference type="KEGG" id="tsr:106556433"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030273">
        <property type="term" value="F:melanin-concentrating hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008361">
        <property type="entry name" value="MCH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004047">
        <property type="entry name" value="MCHR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF91">
        <property type="entry name" value="MELANIN-CONCENTRATING HORMONE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01507">
        <property type="entry name" value="MCH1RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01783">
        <property type="entry name" value="MCHRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="55"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013930904.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="39508" checksum="978B19FB8875EEB4" modified="2020-10-07" version="1">MDFQANQTSNDSDSILNISQLGPGFPPDGDLGSTSYSTVIMSTLFGIICLVGVVGNSLVICAVLKKSSPGSTVPDIFIVNLSLVDLLFLLGMPFLIHQLLGQGSWKFGGTMCTIITALDANSQFTSTYILTAMSIDRYLATVYPFSSTRFRKPSMAIAAICLLWALSFLSITPVWMYAQLIPLPGELLGCGIRLPDPQRDIYWYTLYQFFLAFAIPFTVITVAYQRILLKMARSSEALTRQRGTQLRIKRVTRIAIAICLAFFLCWAPFHALQLVQLAVEPTLPFYYAYNVAISLGYASSCLNPFIYILLGQNFRRRITVSDQPADVEGLFQHQVKSGPGDPSESGQPLLHLVSVLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3Y9</accession>
    <name>A0A6I9Z3Y9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc metalloproteinase-disintegrin-like NaMP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106556476</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930944.1">
        <property type="nucleotide sequence ID" value="XM_014075469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3Y9"/>
    <dbReference type="GeneID" id="106556476"/>
    <dbReference type="KEGG" id="tsr:106556476"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018358">
        <property type="entry name" value="Disintegrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF32">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="13">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="12">
        <location>
            <begin position="110"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="17"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="59"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013930944.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="35304" checksum="D7CC20650F01B12B" modified="2020-10-07" version="1">MVFEHGTLGLAYTGGMCDSFNSVALIQDNTLYAILTAGTMTHEMGHNLGMDHDRNFCTCNKRPCIMNPYDSFDPPHEFTSCNLWDYQYYLTAQDAQCILNDPLSTDIVTTTVCGNGFVEAGEDCDCGDPEQCENECCDAATCKLNPGAKCAEGACCEKCQFKKAGEVCRATKDDCDLPEHCTGQTAECPIDHFHKNGHPCRNNLGYCIKGICPTLADQCIALFGPDAKVAPDECFENNQKGDDINYCKKQNETKIPCEPQDVKCGRLYCTVDSTDENSCKYHFSKGNPNNGMVYIGTKCAEGKVCDFEKCDDLETVFGPTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5B3</accession>
    <name>A0A6I9Z5B3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">UBL3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931050.1">
        <property type="nucleotide sequence ID" value="XM_014075575.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Ubl_UBL3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like 3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040015">
        <property type="entry name" value="UBL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047977">
        <property type="entry name" value="UBL3_Ubl_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032572">
        <property type="entry name" value="MUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR032572"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
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            <dbReference type="PROSITE" id="PS50053"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931050.1"/>
        </source>
    </evidence>
    <sequence length="117" mass="13130" checksum="6CAB97002B307962" modified="2020-10-07" version="1">MSSSVPSDMINLRLILVSGKTKEFLFSPNDSAADIAKHVYDNWPMDWEEEQVSSPNILRLIYQGRFLHGNVTLGALKLPFGKTTVMHLVARETLPEPNSQGQRNREKTGESNCCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7N3</accession>
    <name>A0A6I9Z7N3_9SAUR</name>
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        <submittedName>
            <fullName evidence="8 9 10">Histamine N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10 11" type="primary">HNMT</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
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            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931874.1">
        <property type="nucleotide sequence ID" value="XM_014076399.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931875.1">
        <property type="nucleotide sequence ID" value="XM_014076400.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931876.1">
        <property type="nucleotide sequence ID" value="XM_014076401.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931877.1">
        <property type="nucleotide sequence ID" value="XM_014076402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7N3"/>
    <dbReference type="GeneID" id="106557223"/>
    <dbReference type="KEGG" id="tsr:106557223"/>
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    <dbReference type="OrthoDB" id="5984880at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000118">
        <property type="entry name" value="Histamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016673">
        <property type="entry name" value="HHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016616">
        <property type="entry name" value="HHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51597">
        <property type="entry name" value="SAM_HNMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR016616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013931874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013931875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013931876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931877.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="33481" checksum="C50FDC2AA20E1B8C" modified="2020-10-07" version="1">MAMRSLFLNHSHYFEAFNIFLKKSTEHQCMLDFIQKKLPQLISSISNGKTEINILSVGGGSGEIDLEILARLQSEYPGVIIHTDVIEPNAEQINSYKESVAKASDLKNIKFTWHKETSLEYENRIMDSKEMGKWNFIHMIQMLYYVKDMPATIRFFHSLLEDGAKLLIILVSGHSGWAKLWRKYGPRLPPNDLCLYISSVDVEEMLDSSGLKYQTYELPSDMDITECFTEGNKTGELLLDFLTETYNFSKTAPLDLKNELTEDLKKPEYSERRGDKYFFNNTLSAIMIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WWT8</accession>
    <name>A0A6I9WWT8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger protein 346-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106537713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907384.1">
        <property type="nucleotide sequence ID" value="XM_014051909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WWT8"/>
    <dbReference type="GeneID" id="106537713"/>
    <dbReference type="KEGG" id="tsr:106537713"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052644">
        <property type="entry name" value="ZMAT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46786">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46786:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="2">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907384.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="17128" checksum="F8913A72A5782006" modified="2020-10-07" version="1">MKNSLGSRGEKLLGAKSQPRSERMKKPGSPTSSQDNISYTSDFMYFHESDAELTPKNSEEEKEREKKHTSFTLCNVCNIQLNSAAQAQIHYHGKSHQKRLKQLSNGNLKNSFVRARKKSWHRTMCTNCNSEHITEGCRKRCKCMLSFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZF7</accession>
    <name>A0A6I9WZF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">40S ribosomal protein S9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RPS9</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908359.1">
        <property type="nucleotide sequence ID" value="XM_014052884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZF7"/>
    <dbReference type="GeneID" id="106538405"/>
    <dbReference type="KEGG" id="tsr:106538405"/>
    <dbReference type="CTD" id="6203"/>
    <dbReference type="OrthoDB" id="1697570at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000021">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10 15" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="12">
        <location>
            <begin position="108"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="162"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013908359.1"/>
        </source>
    </evidence>
    <sequence length="194" mass="22608" checksum="067E3CBD58E24F34" modified="2020-10-07" version="1">MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDQRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGGGDDEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X328</accession>
    <name>A0A6I9X328_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Myopalladin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MYPN</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the myotilin/palladin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909694.1">
        <property type="nucleotide sequence ID" value="XM_014054219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X328"/>
    <dbReference type="GeneID" id="106539427"/>
    <dbReference type="KEGG" id="tsr:106539427"/>
    <dbReference type="CTD" id="84665"/>
    <dbReference type="OrthoDB" id="6612025at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001777">
        <property type="entry name" value="myopalladin isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000761">
        <property type="entry name" value="palladin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001108">
        <property type="entry name" value="palladin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF13">
        <property type="entry name" value="MYOPALLADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="150"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="742"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="841"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="256"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="348"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="412"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909694.1"/>
        </source>
    </evidence>
    <sequence length="989" mass="109288" checksum="3E33EBF3F2F72B89" modified="2020-10-07" version="1">MSYRWYCEGKELENSPYIQIIQSGELHSLIIVEAFEEDTGRYSCFASNIYGTDSTSAEIYVEGASSSESESDSGKEEMDRYQNQTKTSSTAPPPAPLPPTPLLANSSATHQESIQPQPLMKLPVSVPVYQNQSPTNYLQGLDGRPLIAAPVFTKMLQNISASEGQLVVFECRVKGAPSPKVEWYREGTLIEDSPDFRILQKKPRSMAEPGPVDQTSNAALHAISSLGLVASEHQPLAPTPSLSLVDNPTKPKLESVLVNHNEPRSSSKIGLRVHFKLPEDEKGSETSSEDGSGPKNQARPNSFPNKINGQAFKLQEPSSPSKEPPPVLAKPKLDATQLKQLHSQVLFEQQQIHQPSHPVPPESLFNTGPGKSATLPIQHQHQKQPSSATYKQNKVPSTQTFTYTRPKQFLASQNTSAGSSPSSSSVSSFSNVPLGTQKTTKKENLFASPPPAQSKSLEGLTNQMEQPLPSPREHVLPPLLLSVSNMNQFHPQNVLPTPLSPTGRIQNPVAFLSSVLPALPSTPPTNAMGLPKCAPAMGSHGMLKKNLNPSQSSTEDAAVAKGLEKRLHLWNDESFRSQQTRNTKLEVAWKLIPQLQTVPPDIFLETFIEGITKGNMEPQDISHTRIIVINTRLLTIDVSLKIPQVYWFKDGKQISKKNGHFKMIREGDGTCSLHIETTTNDDDGNYTFMAANPQGRISCSGRLMVQTPSLRGRLSTTIQSHRGRSRVSERDREPLQERFFRPYFLQAPGDMVAHEGRLCRLDCKVSGLPLPDLMWLRNGKPVLPDATHKMLIRENGVHSLLIDPLRQSDAGIYTCIATNKTGQNSFSLELSVVEKEVKKAPVILERLQNCGVPEGHPVRLECRVIGMPPPIFFWKKDNETIPPHRERMSMHQDATGYVCLLIQPAKKVDAGWYTLSAKNEAGIVSCTARLDIYAQWHQQISQPIKFRSSSTRYAHLTGQGLDIFSAFSTVENPARFPSPSRPVVESEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X421</accession>
    <name>A0A6I9X421_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cyclin-dependent kinase</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">CDK2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Nucleus</location>
            <location evidence="20">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013910064.1">
        <property type="nucleotide sequence ID" value="XM_014054589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X421"/>
    <dbReference type="GeneID" id="106539726"/>
    <dbReference type="KEGG" id="tsr:106539726"/>
    <dbReference type="CTD" id="1017"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000675">
        <property type="entry name" value="cyclin-dependent kinase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000599">
        <property type="entry name" value="Cyclin-dependent kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF521">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13 26" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0469">Meiosis</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013910064.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29883" checksum="898E38EEDAFFFEE1" modified="2020-10-07" version="1">MENFQKVEKIGEGTYGVVYKAKNKVTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDSSTSISGVELPLIKSYLFQLLQGLAFCHSHRVLVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMLTRRALFPGDSEIDQLFRIFRTLGTPDETVWPGVTAMPDYKSSFPKWARQDFSKVVPPLDEEGRKLLALMLHYDPNKRISAKTALNHPFFRDVTKALPHLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5Z9</accession>
    <name>A0A6I9X5Z9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="26">Aminopeptidase N</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ANPEP</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909252.1">
        <property type="nucleotide sequence ID" value="XM_014053777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5Z9"/>
    <dbReference type="GeneID" id="106539094"/>
    <dbReference type="KEGG" id="tsr:106539094"/>
    <dbReference type="CTD" id="290"/>
    <dbReference type="OrthoDB" id="510539at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000005">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000012">
        <property type="entry name" value="Aminopeptidase N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000016">
        <property type="entry name" value="Glutamyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF172">
        <property type="entry name" value="AMINOPEPTIDASE N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 26" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="24">
        <location>
            <begin position="117"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="22">
        <location>
            <begin position="349"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="23">
        <location>
            <begin position="654"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="19">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013909252.1"/>
        </source>
    </evidence>
    <sequence length="1001" mass="113604" checksum="F30B6A0B3856BF01" modified="2020-10-07" version="1">MAKGFFISKSLGIVGIVLGLGAVATIIALSVVYTQEKQRADNALGNQGPSTTLTAGATVTSTLQATMTSTTRGTTASTPRGTTTSTIQATTAGANSTIPTKPLEPWDHMRLPKTLMPTFYTVSLQPFLTELRPNFYIFKGKSTVEFQCKQPTDLILIHSKKLNYTLQETFLASLRGVNVAAPPIERTWLVEKTEYLVVKLKEKLLQNETYQLHAEFTGELADDLAGFYRSVYTEDGVDKLVATTQMQAADARKAFPCFDEPAMKAVFTITLLHPPAYTALSNMPVNKTEKVMSDGEIWMRTEFEPTLKMSTYLLAFIVSEFENVSAVPNNTQLNIWGRPKSIREGQGDYALNATWPILSFFEDEYHVPYPLKRLDQVALPDFNAGAMENWGLITYRESALLFDPEFSSIGNKERIVTVIAHEVAHQWFGNLVTLEWWNELWLNEGFASYVEYLGANKAEPTWNIKDLIVPNDVYRVMAIDALASSHPLSSPADEINTPAQISEVFDAISYSKGASVIRMLSEFLTESVFGEGLQSYFETYQYGNTVCDDLWEQLQMVVYKRNMSLPDSVKAIMDRWTLQMGFPVLTVNTTTGTITQKHFLLDPESPVERPSPFNYNWIVPVSWLSKGKEAEMYWLRNISAENANFSTSADPAKWLLLNVNVTGYFRVNYDLENWERLMNQLNEDLQMIPILNRAQIIDDAFNLARAKHVGSDLALNTTRYLAQEREYLPWDTALNNLDYFRLMFDRSEVYEPMEKYIRKQIIPLFDHFQNLTRNWTQIPEGLMDQYNQILAIRTACSYGVPKCNELASSWFNDWKANPSINRIPPNLRSAIYCSAISTGGLEAWDFAWEMFQKAQVISEADKLRSALTCSKTPWILHRYLQYTLNSTLIRRQDATSTINSIASNIVGQPLAWDFVRVNWRTLFQQFGGSSFSFSSLIQSVTQRFSSPLELEQLKKFKTDNADVGFGSATRALEQALERTKANIKWVADHKPLVLQWFRENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X656</accession>
    <name>A0A6I9X656_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Actin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106539131</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909307.1">
        <property type="nucleotide sequence ID" value="XM_014053832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X656"/>
    <dbReference type="GeneID" id="106539131"/>
    <dbReference type="KEGG" id="tsr:106539131"/>
    <dbReference type="OrthoDB" id="9862123at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013909307.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="44246" checksum="12BF50226FC3E3BD" modified="2020-10-07" version="1">MNRHKKAASPGPSGHRPGSKAPASTSKSSPSDKYKDYAAVVIDTGTGYTKSGLAGDEKPRSIVPSVVGVPRFRTKESPLYYIGKNIPQQRAEVSTHLVMTHGVVTDWDALEMLWHHIFYTELSVCPEELAVLVTDAPMSPTTNREKMAELLFENFEVPAMFVAHQSLLSVYSYGRTGGLVIGSGYGTSYTAPVHNGYILPHATYRLDIAGNALTEYLAKIMGESGNPFQKEEMEVVCQIKEKCCYVPEQYEAELNADEKKYLMDYTLPDRQVISIGSERFRCPEVLFNPTMLGYPEVGLHVQAQNSVKKCKPEREADLLSNVLLAGGTTMLRGFSERIKMELQNLEPGSKVGILASPNRTFSAWLGGSIVASLNAFQNVWINRQAYDEKGPFIVHRYCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6H5</accession>
    <name>A0A6I9X6H5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NACHT and WD repeat domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NWD2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013906993.1">
        <property type="nucleotide sequence ID" value="XM_014051518.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106537406"/>
    <dbReference type="KEGG" id="tsr:106537406"/>
    <dbReference type="CTD" id="57495"/>
    <dbReference type="OrthoDB" id="2325716at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001157">
        <property type="entry name" value="Leucine-rich repeat and WD repeat-containing protein KIAA1239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000727">
        <property type="entry name" value="NACHT and WD repeat domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052752">
        <property type="entry name" value="NACHT-WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057588">
        <property type="entry name" value="NWD1/2-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871">
        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871:SF39">
        <property type="entry name" value="NACHT AND WD REPEAT DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25469">
        <property type="entry name" value="WHD_NWD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026927609" evidence="3">
        <location>
            <begin position="20"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="195"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="NWD1/2-like winged helix-turn-helix" evidence="5">
        <location>
            <begin position="420"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013906993.1"/>
        </source>
    </evidence>
    <sequence length="1525" mass="173088" checksum="CFF3C2398B9242F1" modified="2020-10-07" version="1">SPRTLDVFLFSFASSLLSAIEDEFDFALGKQTPAFLKKCVCYIRKIANIERFVKMPEMEKYMDVLHSGGKFLRDPEALEKLIRLRDEFVPTIVASSNLRVYTSVTHCDIKYGYTQEIESHYIEGLGKQFYEDMIDIIQATVQQNFDIETDWIYDEVLQHSSLCRTYSSFYEYRCEARTIMRKYLLPKKMGHVNPLIIYGGPCTGKTFLLAEVSKKAYAWLKEEMGPESDPVVIIRFIGSTEMSTDLRTLLQSICEQLVVSYRCLVQSYPKKTHDLRDLFVNLLNESSFHRPLVLIFDALEQLADHEDARKLWWLPVHLPRSVRIILSTLPNKHGILQKLKSLIHDEDSYIELTPRDRKMCSQVLKQQLLQVKRKVTSGQQIYVNEAFSKCTLPMFVNLTFQEIRRWRSHKDVNESSLCVTVHDGIEQMFWSLQNICGARLLSRALGYITMSKAGLSEMELEDILALDNTVMLELSQRIRNLNPLRVPYIYIAKLKEGLTGYLVERQVKNVTLLVWANRHLQLIAQKIYLHSEEDVHDMHAVIAEYFLGVWSGGRRKSLYGEDQYLNGCLDSDSRSMNNEERHMMDLMNFDRQSPDQPWVFQCNPLEPDIFFINHRKMTELLHHLTKSGKSDDILYGVIMNFSWLYTMIKIGQFDKALSDVELAYNYSQEKELKFLAAILRAIKYKVIKYPGTLSAELQQRLLPVVSSLPKLRQLLLECDKDGPKYCSIVPLHSAMDITYSPERLPLSSSCSQVTEILPTFNPNVVIVALENGSISTWDIETRQLLRQITTAQSVILGMKLTSDEKYLVVATTKNTLLIYDNLNSCLLSEVEIKGSKHSGIGVSSSFINGFALSVNHALSWLEASKDITVIDLLYGWPLHQFHCWYEVTCVQCSGDGMYAFCGQHLNTASIFHLGSGDKLATVTSEFSGGFVKFLLVLDAAQEMVMVDSEGALSIWNTEEIAHPQLMDDFDCRRGDSEFVSLELAEDQSAILICKSLGIDLLDTSMWKVAEKFRAKRNERFVSATLSKNGDYIIASIENTSAIFIWRRDSGQCIATLQEISGNIVRLMKSNHHNMLLSLSTSGVLSVWDIDIITAMSNIDKTGKPIQRMVLPAKGEAIYTLDGSEAIHKWNFSTGFIEAIFKHESLVENCVLTSNGKLMITSDDKCSQYVWQTDSGENLFRINGQRICQLLITHNDQFVVSLCEQNASRVWRLSTGHRVCNILVALQNAFITTANTFVVGMTKNKVLVVSLWTGSITKKFSCDDGITIVDIKLIPDCPDIVVFITSAETVNIYSLSEEVVSRRLQLPNNFLKTLEDFEIAPNGKLGILSCGDENINILDLHSGKLRVVHASGIIWRQRLSQDGRYLVYICFRSGEDDEDNSAISILIVMRLADGKNIGACSLYKTPAFLTLSQRHLNIIVGFDDGSIATYTVVDRVDAALKIKIATSNSRQIFNNASQMMKPKWHHYSFKATADCIWRESTEVFARDSPITVVDVETSDATPTKKHNYCYDKVCSAIDCRVFTAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6H9</accession>
    <name>A0A6I9X6H9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106539576</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909861.1">
        <property type="nucleotide sequence ID" value="XM_014054386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6H9"/>
    <dbReference type="GeneID" id="106539576"/>
    <dbReference type="KEGG" id="tsr:106539576"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="47"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909861.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="38953" checksum="11873D0318DC0E32" modified="2020-10-07" version="1">MEEILLIENGNKTTITEFILLGFGNSPQLQPFLFIFFLVIYLITITGNIFIFLLVLVDHQLHTPMYFFLANLSFLDTCYSCILLPKMLVSLLTGEKTIAVHRCLVQYHFVCLCGATETYILAAMSYDRYLAICRPLHYVSIMNKKLCFQLMAGAWINSLIFDVILVGFTTQLSFCGPNVIDDYFCDFRPLQKLSCTDTSLIELIAFCEAVIFIIVPFLLTIASYICIIGTIIKIKSTTGRQKAFSTCSSHLLVVSLFYSTLIIVYVLPDTPSLRPLNKIFSIFYTVMTSLFNPLIYTLRNKEVHKAVKRLFNKILMHHMNHLKFSTSLHFISLQKFHCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7D4</accession>
    <name>A0A6I9X7D4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transport and Golgi organization protein 2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TANGO2</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013907293.1">
        <property type="nucleotide sequence ID" value="XM_014051818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7D4"/>
    <dbReference type="GeneID" id="106537632"/>
    <dbReference type="KEGG" id="tsr:106537632"/>
    <dbReference type="CTD" id="128989"/>
    <dbReference type="OrthoDB" id="191601at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013907293.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="25223" checksum="24C2D2129CB8E04D" modified="2020-10-07" version="1">MEEGKEGGSWLGISKKGKMAALTNYMQPQINKHAKGRGALVTNFLTSEMDSYSYLKKVASEGHLYNGFNLIAADLSTTNGDVIYYYGNKGDPEPVFLNPGVYGLSNSLLDTPWKKLQYGKQLFSDVVKHSQNLKKEDLVQELIKLLNNQDPQLPDPAMEDQGKDYVLPFLNKYAALCVRCPDYGTRANTIILVDGEGHVTFTERAMLNADITQWKSSTYHFQLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8B7</accession>
    <name>A0A6I9X8B7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 850-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106537832</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907568.1">
        <property type="nucleotide sequence ID" value="XM_014052093.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106537832"/>
    <dbReference type="KEGG" id="tsr:106537832"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000136">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000008">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002129">
        <property type="entry name" value="Zinc finger protein 304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000281">
        <property type="entry name" value="Zinc finger protein 558 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000895">
        <property type="entry name" value="Zinc finger protein 597"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000785">
        <property type="entry name" value="zinc finger protein 648"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="548"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="604"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="632"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013907568.1"/>
        </source>
    </evidence>
    <sequence length="702" mass="79460" checksum="15EAD2917F2139F2" modified="2020-10-07" version="1">MLASMATWTHTEEKLFECPDCEKCFCDNSNLMIHQRIHTGEEPFKCPDSGNSFSQNSHLVSHHSTHTREKPFECSVCQKSFSKNSSLVTHQRTHTGEKPFKCPDCGKSFSQQSSLVTHQRTHTGEKPFECPDCGKSFSHNSSLVKHQRTHTGEKPFKCPDCGKSFSQNSSLIGHQRIHTGEKPFKCPDCGKSFXDLSSLVKHQRTHTGEKPFECPDCGKSFSTNSSLVTHQRTHTGEKPFKCPDCGKGFSQNSNLVTHQRTYIRKKLFECPECEKCFCDNSNLVIHQRTHTGEKPFKCPDCGKGFSQNSNLVTHQRTYIRKKLFECPECEKCFCDNSNLVIHQRTHTGEKPFECPDCDKCFSTSSSLVRHQRIHTGEKPFECPHCGKSFNTNSSLVTHQRTHTGEKPFECSDCEKCFGDNSSLVRHQRIHTGEKPFECPVCGKSFSNNSTLVTHQRTHTGEKPFKCPDCGKSFSHSSYLIGHQRIHAGEKPFECPVCGKSFSQNSHLIEHQRTHTGEKPFKCPECGKSFSQNSTLVQHQRIHTGEKPFECPDCGKSFSQNSTLVQHQRIHTGEKPFKCFDCGKSFSQNSTLVQHRRIHTGEKPFECSICGKFFSQNSHLVEHQKTHTGEKLFKWPDCGKSFSKNFKLVTHQGTHIEERPFECPDLGKVSLGDPSSCTSGLTQERNCMSVLFVENVSAAIQTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9H8</accession>
    <name>A0A6I9X9H8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM227A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FAM227A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM227 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910412.1">
        <property type="nucleotide sequence ID" value="XM_014054937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9H8"/>
    <dbReference type="GeneID" id="106539987"/>
    <dbReference type="KEGG" id="tsr:106539987"/>
    <dbReference type="CTD" id="646851"/>
    <dbReference type="OrthoDB" id="192208at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029417">
        <property type="entry name" value="FAM227"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33560:SF1">
        <property type="entry name" value="PROTEIN FAM227A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33560">
        <property type="entry name" value="PROTEIN FAM227B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14922">
        <property type="entry name" value="FWWh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="344"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013910412.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="66366" checksum="579266E269C77FE3" modified="2020-10-07" version="1">MNFIHTKAFLSPLSGFLILPPRTQEERSKTFSCSLDDLPRLYVIGNIQNVNRKISTLELGLRRYDSSDVVIEDMMHRKYPGLRSPRKGRRISRGKEKDQAMTHKYGRLGSDAETLQNKKKIAHKKMITEKNKLVELYHYPGYHEEELTPLPNGIDLNEILEKVIRAQSKTVVGKTKWAVKMLHRFFDTPCSRAVLLDSFWWQFLHLYHPNQEIQGHLFERIAENYALILLGDHRASNQECILKFFPSLLSQAVYVSFCSCFPRSWFNTSEFKTQLCNVFFEWLGGTRHEPRSFYKWDYSQLEPERSRREDLMSRKKKIGMGISFESYKQYSSIGKTRLQKIPSRKTITKSTKKGKKTVPGKKDQKQPSKKVTKLESEEIKQSTSIIDQLKEDNEKAAWSKHQNLPKKKLKIAKLPRESHPACPGPDFTWHRFNISGNSPLIQHYFQKRGIESKSGCDIFLSRREIDKSMPDSAKTYAELIKEGFHTVHQKHKAFRKYYWQQYKEMRKFDEQNKCNQELYREAIKEEMKKRKAQKQKEMLSFRERQNSSEPSSLFSRDSSGKRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBP4</accession>
    <name>A0A6I9XBP4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trinucleotide repeat-containing gene 6A protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TNRC6A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GW182 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912994.1">
        <property type="nucleotide sequence ID" value="XM_014057519.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541939"/>
    <dbReference type="KEGG" id="tsr:106541939"/>
    <dbReference type="CTD" id="27327"/>
    <dbReference type="OrthoDB" id="5919166at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035195">
        <property type="term" value="P:miRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000011">
        <property type="entry name" value="trinucleotide repeat-containing gene 6A protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019486">
        <property type="entry name" value="Argonaute_hook_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052068">
        <property type="entry name" value="GW182_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032226">
        <property type="entry name" value="TNRC6_PABC-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13020">
        <property type="entry name" value="TRINUCLEOTIDE REPEAT-CONTAINING GENE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13020:SF28">
        <property type="entry name" value="TRINUCLEOTIDE REPEAT-CONTAINING GENE 6A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10427">
        <property type="entry name" value="Ago_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16608">
        <property type="entry name" value="TNRC6-PABC_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="1741"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="304"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="393"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="547"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="723"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1006"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1209"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1284"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1376"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1537"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1608"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1829"/>
            <end position="1873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="270"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="403"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="504"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="680"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="896"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="925"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1284"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1392"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1537"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1557"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1615"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1829"/>
            <end position="1841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013912994.1"/>
        </source>
    </evidence>
    <sequence length="1922" mass="204905" checksum="61863DABE63F82F6" modified="2020-10-07" version="1">MREWEAKATKDAERKPSRELVQEEEEQLMEEKRKRKDDKRKKEAAQKKVTEQKSKVLEPPKPGTSQPLPASTPPTTSIVTGSVSGNNNAKRAVGNNPQAQQPSSPRYPPREVPPRFRHQEQKQLLKRGQPLPGIAAKLRAPSKLLNSQAGESTGTREQPVSESQGQSSSQKQPDLNHSTLGSHYENAHWERTFSSSDSSTNWDKTVVDSSEKEAWPSTTGSDLQLASECMDADSASSSGLERNIMMASRGPSSDVQHLQNGIGHGNQAKLVSSSNSNNVSSGSLSGSWGASLGALMSPCPLSAEASKGIPESSHGRANAWGAPSSSNEESNPSTLTSNGHGGAWPVLESSGPALKGPRGSSSCGTDAVQMVNNQSANSSLASSWGSLQENCDSQLHGTRKLSRSGQPQNPNHEVNGPNKNTTNFMTSSLPNPAGSMQTTEPVSNAGPGTWSRSAKSKRPQPSASPLTNGTSISHLSNGDTKNGGAPGSRWVTYGPGYSLETRSDSNSQANNDTVNATPMHPGLTRTSSSAIEISEDQQTGTWEVAGMGNSQNGTWGPRSGMGHGGGRRGSAQNANLGDGEWNKLPSGHSSEANSGNGKMFTNEWKSAEGEKADPQCCTAPQALEQNGGWTKASSEAGDGNLEVPEERSSAEGTSHERRKVDQQALLQSMVNRTDLDPRVLSNSGWGQTPIKQNTAWDTEVTPKGGKKADNGTEAWVGCVAQACSSGGAPERPHLHGPSPSPGTGWGEPKSASRWGDSRGPSSQGGWEEGAAPVMGKSSQSWGTGQEVRAPSLTWNDPEKLRQGWGDGPKASSGWAAPAGDGWGENVRNNHWGEMKKSSSSSSDSDRSTSCWKEPARSNSGRWAGGNGGSTKPCSASGWEETAKPNPSPVWGETPKPNRSQSWGEPSKPSGSPDWSKQQEAGYWGGASATAAAAAGKPSGVGWLGRPMPAPVKEEEPTGWEEPSPESIRRKIEIDDGTSAWGDPSKYNYKNVNMWNKNTPNPVGRLDVQTHQPGLPSGPAAGTESVASSSSGWGEPPPAPATVDNGTSAWGKPVDTGRSWGEPVSEATDARGWGNASVGQQASHKAGPKSMQVPDGWCGGDMPLTGNHPASWEEEEDVEIGVWNSNSVQDASAPLHWPPCSKKMPSKAAMKSGNKQDEMWTNPFVKQFANLGFPRDLPEEMMQSNKMELSGGILPDKRMELEKHNLNVGEGSRAIGKNPGSHPQISKESSMDRSPYFDKDGLVADDSPNMQFMPSQNGKFPPSNSALPNHVLSSLAGLNVQSLNSVRQNGNPSPFGNLTAQARTMQPSPAQPPLHSSQSNPRAQVPPPLLSPQVPVSLLKYAPNTGGLSSLFGPQQVAMLNQLSQLNQLSQLQRLLAQQRKMQGQRSTPAGGRQEQQQQQQQQNRPHNMQQQMMPPSCQLDPSLLMKQQVPPSQQQFHQTAIKSFLENVPHANQELQKGPSQINAFSNFPIGMNANLNVNMDMGSIKEPQSRLRKWTTVDSISANTSLDQNASKNGAISSGFRMEETSSFVPYDFMSSSNSPASPPGSVGDGWPRAKSPNSSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVTNSLSINTVREADHLRDRNSGSSSALNTTLPSTSAWSSIRASNYNVSLNSTAQSTSSARNSESKSTWSPASVNNTSLAHELWKVPLPPKSITAPSRPPPGLTGQKMPLPTWDNSVRFGGGWSHSDARYTPGTSWNENSSSGRITNCLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVMKAQKSLHMCVLGNTTILAEFASEEEISRFFAQGPSLTPTPTWQSLGTSQTRLGSMDSPHAFSNRSDLTPPWSGPGLSGTSSGELHGNSLWGSPNYSTSLWGSLGGNDTRGLGSPSPLNAFLSVDHLGAGGESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD13</accession>
    <name>A0A6I9XD13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Delta-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">DLL4</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Putative Notch ligand involved in the mediation of Notch signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913489.1">
        <property type="nucleotide sequence ID" value="XM_014058014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD13"/>
    <dbReference type="GeneID" id="106542323"/>
    <dbReference type="KEGG" id="tsr:106542323"/>
    <dbReference type="CTD" id="54567"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035282">
        <property type="term" value="P:segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035239">
        <property type="term" value="P:tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000064">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.140:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3510:FF:000003">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000247">
        <property type="entry name" value="Delta/notch like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF22">
        <property type="entry name" value="DROSOPHILA CRUMBS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01414">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 15" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11 13 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 13 15" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026746339" description="Delta-like protein" evidence="17">
        <location>
            <begin position="23"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="532"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="DSL" evidence="19">
        <location>
            <begin position="169"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="280"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="320"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="358"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="398"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="436"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="477"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU280815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013913489.1"/>
        </source>
    </evidence>
    <sequence length="685" mass="75555" checksum="13CEFE623383B5CA" modified="2020-10-07" version="1">MAALRLLGFALALALPLQRVCGSGVFQLELREFVNGQGFLASGESCGVGCRTFFRVCLKHFQAVISPGACTFGSLTTPVLGTNSFAIKETEGFGSPIKLPFNFTWPGTFSLIIEAWHAPEGYYPTGSETPARKWLISQMAIQRSLAVGEDWSQDEQSNQRAKLSYSYRVICNEHYYGDNCSRICRSRDDLFGHYLCQPDGSLVCMAGWTGNYCTEAICLAGCSEKSGFCRNPGECICRNGWRGRYCDECIPHVGCRHGTCTKPGKCLCDEGWGGLFCDQDLNYCTHHKPCQNGATCMNTGQGSYTCSCKEGFTGKDCERNISECDSSPCKNGGSCTDLGNEYKCTCLPGYDGINCEHTTLTCLESPCFNGGTCLEKEKGTSYECLCRMGFIGSNCEKKVDRCTNNPCANDGQCFVMGQQHVCRCRQGFSGQTCEIHISRCSRNPCFHGGSCQDLDAGRDYKCTCLPGFTGRNCEIRTRDGCSSGPCQNGGTCYTGLYSFTCHCPSGFLGIHCQFAVNPVTSIIPPLPKPVPWVAISMGVGLVALLILCCMIAIVIRQMQRHPEQNSETMNNVSDFQKENLIPASQLKNTNKNKDLEIDCALEKSNYKLKNHTLDYNLVKELTNRGTQEDKYHKSEKCIGEKSPFWLCSEKPECRISAICSPRDSMYQSVFVITEERNECIIATEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDJ9</accession>
    <name>A0A6I9XDJ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">GDNF-inducible zinc finger protein 1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106541126</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911942.1">
        <property type="nucleotide sequence ID" value="XM_014056467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDJ9"/>
    <dbReference type="GeneID" id="106541126"/>
    <dbReference type="KEGG" id="tsr:106541126"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004075">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004271">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001818">
        <property type="entry name" value="GDNF-inducible zinc finger protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002711">
        <property type="entry name" value="Zinc finger protein 16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001443">
        <property type="entry name" value="Zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF393">
        <property type="entry name" value="CHROMATIN-LINKED ADAPTOR FOR MSL PROTEINS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="396"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="609"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="638"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="668"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="696"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="724"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="752"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="780"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="808"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="836"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="452"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="463"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="493"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013911942.1"/>
        </source>
    </evidence>
    <sequence length="935" mass="106140" checksum="D6120050DB769F74" modified="2020-10-07" version="1">MEKKKISMKSSVAASNLLKTLHSFYEFGHFCDVTIYTDQLGSQQEFYVHKAVLAASSNYFKSIFLNNEMNNVKSCTVILQDIHAEEFVSFLKFVYTAELEIEVDRLHLFKEIAKRFECEDLLEILEEMKVVGGKLEPNVILKKSKGVRLPGNLTELGGEKAQRDSSRVLAAAVMKKNHLGKRTTHSKPTTSFDDIKNICVNPNEDRIASCNSKDESATTSEHISEVDCPSVNEEDLKETHIALSRTLLDQNDESSLTPSKAKLRKSTCNIPKALPRSYACDKCNYLFHFAEQYQSHIELEHSPSLGVKYSCNICSQLFPSCHTLRQHRLTVHSRQGTVPCLLCEKKFQCQKDVNAHMRTVHEKKQHPQQCPYCDKVISSKGGLTVHIRTHTGEKPYKCQCCPASFAQRSAFTTHVRKIHESKKDGKCMPAYWKIVPPAEKVDAIDNRINASNKTQLKVPNNDLPKEILGKATQEPERKSSRSLPEEAQSDNQSNKKLELGTENNAKKEETCRQGTVDEKGNGERVCEAGLCSNKDNEAASSDDNYSEDLNVHDTEDPEWDINCKVNKNKAIPTSAYIITCTKCTEKFPSRRKYVIHCKEIHHSLPGKVYQCDVCSKSFASYNSWKEHQACVHTEDRKFACTLCNATFKRKRDVRTHSVRKHEGRVKRPLCSVCGKVLSSRTALVFHMRTHTGEKPYQCGICSSRFAQPTQLKIHTRSHTGEKPYICEECGASFADKGKLNGHKRTHTGERLFRCDVCGKHFATNEYLKCHKRCHMGAKPYKCDVCGKTFGLRASLAQHSNVHAETPPYFCEQCGKTFTQQGALRRHQRIHTGEKPYKCRACEKTFTDMSTLRRHVLVHDRNAHWRSFLIDLTIKKDHNWSKIEAMSTTCVEENSTTVLSDCQEKCYTSENTNVKQLEQIPGSSNVKESEPTLMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHF1</accession>
    <name>A0A6I9XHF1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Envoplakin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EVPL</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the plakin or cytolinker family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915144.1">
        <property type="nucleotide sequence ID" value="XM_014059669.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543611"/>
    <dbReference type="KEGG" id="tsr:106543611"/>
    <dbReference type="CTD" id="2125"/>
    <dbReference type="OrthoDB" id="9945740at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001533">
        <property type="term" value="C:cornified envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000178">
        <property type="entry name" value="Envoplakin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000010">
        <property type="entry name" value="Envoplakin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.780:FF:000002">
        <property type="entry name" value="Envoplakin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000109">
        <property type="entry name" value="Periplakin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000088">
        <property type="entry name" value="Periplakin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000030">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058847">
        <property type="entry name" value="Plectin_PPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055419">
        <property type="entry name" value="Spectrin_PEPL/EVPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF7">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00681">
        <property type="entry name" value="Plectin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26346">
        <property type="entry name" value="Plectin_PPL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23160">
        <property type="entry name" value="Spectrin_1st_PEPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0417">Keratinization</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="405"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="663"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="905"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="963"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1193"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1415"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1475"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1533"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1642"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013915144.1"/>
        </source>
    </evidence>
    <sequence length="2023" mass="235008" checksum="2FDCDFECC43A33E7" modified="2020-10-07" version="1">MFKGVAKGSPSGSTPTKGSPTKQFKSSSNNDLAVLISRMQKNADQVEKDILETQSKLKKDAINRHQNKPLEFQQINGKNLKDAETLLKDLFLDVDKVKHLKHPQAFEIEKDIKQLHIRMTHQCAEYRELYEKFTLSEVGSKVDWAKILEQKQKQISGGQYGPTMPELEKQIAEHNILQKEIEAYGLQIKNLQSLEAGDLKIQYKDLLKASIWRGQSLNSLYTHLQGCMKELAYLSEQQQKILKQDWSDQMIEPQSVRREYEEFRNNELLNQEEYVNMLQDDGERMIELKHPAVTPIQAHQEALKNEWQNFLNLCICQEHHLKSIENYRKFYDDVDDMKLFLSKLNNDLNNKYSKFNKDNPGVVSNLMCHLENDEKTVKQAEKSIAELKRRSKEISPLKLRRIQCSQPIIIDAICDWDLGEVQLERGEKYTLKNNNNQENWEVQNAKEESKIAPAVCFSIPPPDLEAIDRVNRLEGELNTIKQKRASIQTTLRSSNKESVKPSHQATTRNFTPVQDDSEADHLLHKLNKIDSDLGQIKMDIFNRTRSPLNHSDPIEDVTQRIKHQEGTNKIIQIIGTEKEATQKACETYLSKKPAGTTASQIPVMLNNIKNKYNDVKVLSRLYDEKAKASINLETEIESMDRIISTIETKLSHDGTIPASPNALQDRANELQKLKRDLVKQENCLLKLNRSLKDAEHSCSAVQNNFQEYCPDLPRQKKEVQLINDRYHIVADQLDQREKTLWDTSLIYQQFQNANENLIFWLNNLPKHKVKTTDGPSQINYKLEAQKRLADEIQSKESDKNSVINLSRNVQSILNDYELQAGKYRSSLDPTLTASSAKRLRVTPLQESIQAQENDMTKLYAETAAENKQQICWLEFAKKIMNKKEVSDTVRVKYEQTQQSKNIDESEILKSQLKEEQSRKANVQQELEEQRNKLLTLKTQRPIERIEEKEVIDYYRDPVLESELAKISNHIENEKQKRTDLQTDIEKVNNIFLEMEKGRRVVKSQLLTKEVTRTEKDPNLNAQAARLQEEIRYLRDHSTSSSEIEQLRKELYLLEQKQSNIREKIVVKEVVKLEKDPEMLKAIRSLQMQIDEDSFKKKSAMDTIGKMKSKIEDLEKLIEATEPKVVVREIKQVEQDPELLKESSTLQKLIDEERNKNVLLSSELTQLQSKYSTMEKSKPRVEIKERVNEIFHLDPETEEEIARLKRELQEISRKRKRVDQEVEIALTELKVLRSQAPSVEFKEVIQEVVKTEKSPEILREIDRLKEQLNDLVNANGRFQEQLIRLEGERDEWKRERAKVETKVVTKEVVRYENDPLLEKEAECLRQEVRNILQKRRATEDALYDLQNKYILLERRKPEEKVVVQEVVVTQKDVKLREEHKRLSKSLDEQTSNRRRMDHEVQLLRATVEDQEKLLNIQDDRNKRLALEKEMRQITLRIKEIEESPVPVQEKIIMEEVIKVEKDPVLEQSSTNLRTELGNEKGQVLTLQRECKNLQAQIDVLQKTKSQEKTIYKEVIRVEKDRVLENERTRLWDLLSKERTARQNAEESIRHLKEKIERAEGMKCTWAREEADLQKTQNVTMQEKASLEDELRELEKQKQQKALFLREQTKLLSERTESDRQKKIQFGQEVSRLESDILMEKDNIYEKERTIRELQSRINREELNNETQMRETNLSTKISILDPETGKDMSPYEAYKRGMIDRCQYIHLQELECDWEEITTLGSKGDVSVLLDKKSGKQYSIDDALRFKRITKEEYQLYKEGKIPISEFALLVAGETKQNTCLSLGSIISKSPISSPVFQQRQSFFSSGPPMAFCDDTFPIAGVYDTTYDNKYNIKSALSKKLVDPMTAQKLLEAQAATGGIVDLISRDRYSVHKALDKGLIDNTYTQRLLNAQKAFTGIEDPVTKRRLAVGEAVQKGWITQDNAFPYLQVQHLTGGLIDPKKTGRIPISEAIQTGLISSDLATLLQDESSYEKDLVDPITKEKIHYKEAMARCQKDPLSGLLLFPATSEGYQSTIHSAALPRFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHJ7</accession>
    <name>A0A6I9XHJ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SRC kinase signaling inhibitor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SRCIN1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913347.1">
        <property type="nucleotide sequence ID" value="XM_014057872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHJ7"/>
    <dbReference type="GeneID" id="106542207"/>
    <dbReference type="KEGG" id="tsr:106542207"/>
    <dbReference type="CTD" id="80725"/>
    <dbReference type="OrthoDB" id="6022652at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061001">
        <property type="term" value="P:regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF5">
        <property type="entry name" value="SRC KINASE SIGNALING INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3-like C-terminal" evidence="4">
        <location>
            <begin position="205"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="305"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="498"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="598"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="871"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="964"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1182"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="732"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="873"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="970"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1184"/>
            <end position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913347.1"/>
        </source>
    </evidence>
    <sequence length="1232" mass="137295" checksum="3D80052B57248C81" modified="2020-10-07" version="1">MSSSSSSSDEESSRRDIILAPIPRCMRGLLQILSDPERTSAHMISADDAEYPREYRTLGNGTRRFSNVGLVHTSERRHTVIAAQSLEALTGLQKSEMERKRDAFMDHLKNKYPQHALAIRGQQDRIRDQQPNYWSFKTRSSRHSQGSQPGLADQAKLSFASAESLETMSEAELPIGFNRMNRFRQSLPLSRSTSQTKLRSPGILFLQFGDETRRVHITHEISSMDTLHALIVHMFPQKLTMGMLKSPNTAILIKDESRNVFYELEDVRDIQDRSIIKIYRKEPLYASFPGSHITNGDLRREVVYTSRESSPTRRLNSMSPASHLTSGSPPPVLQSSSPSRSRMSYSGGRPPSYAGSPVHHAERLSNLPPAQGVSPSPSAILERRDVKPDEDLVGKNMVLVKNEGLYADPYSMVHEGRLSITSTQSLAGMGDPFAYPGGLYKRGSVRSLSTYSAAALQSELEDSLYKPNAQIYSDTYGPGMGFRLPPSSPQKIADGRLVDVQQSQSPHSPYSGPPSRSSPVRQTFRKDSCSSVFMESPVNKPRNLSSSGASELFPGPGERPHSGFSSPVPAKDTETRERMEAMEKQIASLTGLVQSALMRSSDGETPSEKTEGTNGGTPPSAHPSKSNVGTPVPGPPPPSTTGTPAGQPTAITRLHMQLHLHDLQQNASELRNQLHQLKKLQLQNQETVKTMLRRTETEISVRVTDTMRKQEDPLQRQRTLVEEERLRYLNEEELITQQLNDLEKSVEKIQKDLMHNHRFISIQELEEKSLVLKQLGDTLTELKANFPSLQSKMRVVLRVEVEAVKFLKEEPHRLDGLFKRCKAVTDTLTEVRKQVDEGVWDSSSNLLNQSPKKVELESDFSRSLDFELPTSPPTNLHDLTSSTDSLGTISFGPSQGQSHASKSSNPSRGSEMIGAKPQTGSEISSKKSMDRSVSVEFLFVRIHFIIHMKLLVFSGYELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVIITSKKESSFMKKAESEELETQKPQVKLRRTVSEVARPASTPPIIASAIKDDDEEDRIIAELEVGWEKGIRLKVRSEVREFCKETILTFNKFREKSHQTGNLQREDSLCRVFQRSSSFIPKFRCDQPVPTTDMWPNGATVTTKGWKSGGGVSIPAMKMVNPASRLKQTQQGSPDKSKHIKQRMEYMRIQGQQQAARSTKDSGTGNETSSPVSEKPTSGRTSIPVLTSFGSRNSSISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHK9</accession>
    <name>A0A6I9XHK9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNAation factor subunit beta isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DFFB</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913362.1">
        <property type="nucleotide sequence ID" value="XM_014057887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHK9"/>
    <dbReference type="GeneID" id="106542221"/>
    <dbReference type="KEGG" id="tsr:106542221"/>
    <dbReference type="CTD" id="1677"/>
    <dbReference type="OrthoDB" id="9943677at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003508">
        <property type="entry name" value="CIDE-N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039729">
        <property type="entry name" value="DFF40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015311">
        <property type="entry name" value="DFF40_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13067">
        <property type="entry name" value="CASPASE-ACTIVATED DNASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13067:SF2">
        <property type="entry name" value="CASPASE-ACTIVATED DNASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02017">
        <property type="entry name" value="CIDE-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09230">
        <property type="entry name" value="DFF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00266">
        <property type="entry name" value="CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51135">
        <property type="entry name" value="CIDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CIDE-N" evidence="4">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="325"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913362.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="42166" checksum="8FDC0B6C7AC766CF" modified="2020-10-07" version="1">MAAKAFKVRAGPAGRRYGLAAGSLAELLRKASRLEPPLVEGCRLGCYEDGRQVSEEAFRALPENSELILLRPGEEWSGYVSEIERFLSAFSSCSPGVVHAAQGLLSGEEAPMRCKLLSDLIQNLSENISAESRKEDEMWFEGVESRFKTKSSYMHHSCESRIRGYMKELGAYVSAVPPALQVKFSGIAEAMLEKLKSEKYNGFYFDRKEETKDRLCTPEGWFSCQGPFDSEDCSWRHSINPYGNKESRILFSTWNLDHIIEKKRAVLPTLVEAVKGCDGKEIDWEYFYRLMFTTDNLKLVHIVCHKKTVHNLGCDRTKIYRKRKTGRKTRPLCGHNLSPRVQLSKTKQKNDTKLSKNKATKKPHPIAKKGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJT6</accession>
    <name>A0A6I9XJT6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Single-stranded DNA-binding protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106544332</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916054.1">
        <property type="nucleotide sequence ID" value="XM_014060579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJT6"/>
    <dbReference type="GeneID" id="106544332"/>
    <dbReference type="KEGG" id="tsr:106544332"/>
    <dbReference type="OrthoDB" id="5600002at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12610">
        <property type="entry name" value="SINGLE STRANDED DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12610:SF30">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04503">
        <property type="entry name" value="SSDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013916054.1"/>
        </source>
    </evidence>
    <sequence length="96" mass="9926" checksum="5DB9B3DAC3704984" modified="2020-10-07" version="1">MYMMNSVGPPPSRPGFSMGPSPEGPMAGMSAMEPHHMNGSLGPGEIDGMPKNSPGSMASIANPPGTPRDDSSSEMGSSFINPFQSESYSPSMTMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJZ2</accession>
    <name>A0A6I9XJZ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome P450 2J5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106540563</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911208.1">
        <property type="nucleotide sequence ID" value="XM_014055733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJZ2"/>
    <dbReference type="GeneID" id="106540563"/>
    <dbReference type="KEGG" id="tsr:106540563"/>
    <dbReference type="OrthoDB" id="3934656at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF375">
        <property type="entry name" value="CYTOCHROME P450 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013911208.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15687" checksum="14EB3E09C3365C22" modified="2020-10-07" version="1">MMDRAQEEEELYKLITMIEFINVGFSSVCWQLYNEVPLVRSLPLPHQTILRTAEKIYVFIQKEVEEHKATLTPGEPRDFTDAYLEEIQKPEKKSSGFEEEQLRVLLSDLFLAGTETTAAALQWTMLYLVAFPEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKJ2</accession>
    <name>A0A6I9XKJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transitional endoplasmic reticulum ATPase</fullName>
            <ecNumber evidence="5">3.6.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Valosin-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC106543085</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 24">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013914467.1">
        <property type="nucleotide sequence ID" value="XM_014058992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKJ2"/>
    <dbReference type="GeneID" id="106543085"/>
    <dbReference type="KEGG" id="tsr:106543085"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF69">
        <property type="entry name" value="TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="21">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="20">
        <location>
            <begin position="125"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="237"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="717"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="315"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="915"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="973"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="984"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="23">
        <location>
            <position position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013914467.1"/>
        </source>
    </evidence>
    <sequence length="1010" mass="111599" checksum="A7A5984E9EE14055" modified="2020-10-07" version="1">MASGPDSKADDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQAKMDELQLFRGDTVLLKGKKRREAVCIVLSDDSCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKVGALQPSVQRGGDSTVRGNRPSPPPTPRGRQGRDSALSIGPAEGLLWGASQTLPAQRERSDGRSFGLRDRVRSDPASSLLLAGQVPQREGSEMRARLYLNLGLIYDSLRNPAMRTSYFKKSIYISEQAHLSEDLFRAYFNLGNIHLREKEHSQAMRCLERARECACKMKEKYMESECYTSIAQVLLGLGDFLAAKRSLRKAYRLGSSQPQQRDSVARNLRYALLAGGPSDAAEGSALSRETGKGDGCSWSTAGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWQDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLDFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGTGGTGGGNVYSEDNDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKZ7</accession>
    <name>A0A6I9XKZ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cullin-associated NEDD8-dissociated protein 1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106543210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAND family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914622.1">
        <property type="nucleotide sequence ID" value="XM_014059147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKZ7"/>
    <dbReference type="GeneID" id="106543210"/>
    <dbReference type="KEGG" id="tsr:106543210"/>
    <dbReference type="OrthoDB" id="6260732at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010265">
        <property type="term" value="P:SCF complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039852">
        <property type="entry name" value="CAND1/CAND2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013932">
        <property type="entry name" value="TATA-bd_TIP120"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12696">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08623">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25782">
        <property type="entry name" value="TPR_CAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="TATA-binding protein interacting (TIP20)" evidence="5">
        <location>
            <begin position="920"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013914622.1"/>
        </source>
    </evidence>
    <sequence length="1111" mass="123892" checksum="19FBC22CAA05673C" modified="2020-10-07" version="1">MTANVCKNITAQLTGAIGKQEDVSVQLEALDILSDILSRLGGTLYSFHSSILNCLLPQLISPRLAVRKRAIIALGHLVLTCNGNIFAELMEHLLSELKRNNSTSTTRTYIQCIAGISKQAGHRIGDYLEKMIPLIIQYCNVEDDELREYCFQAFESFVRRCPKEIGPHLPNVTGLCLKYITYDPNYNYDNEEEDEDEIMETENGEDEEQESDDEYSDDDDISWKVRRSAAKCLEAIVSTRHDLLQDFYKTLSPALISRFKEREENVKADIFSAYISLLKQTLPIQSWLQASDEAGKEDIPLTMLQNQVPNIIKALHKQLKEKSVRSRQGCFALLTELANVLPGCLADHIPALVPGIVFSLTDKSSSSNMKIDTLTFLHVVLCNHPREVFHPHVKALLPPVVICIGDPFYKITSEALLVTLQLVKVIRPLDAPCTFDPKPYVTDLFSATLKRLKAADIDQEVKERAISCMGQIVSNLGDYLSSELQPTLKIFLERLKNEITRLTTVKALTLIANSSLKIDLRPILAEGFPILASFLRKNQRALKLSTLTALDVLIRNYSDSLKPAMIECVLMELPDLISENDMHVSQVAIVFLTTLAKVYPSSLSKISGTVLSELFQLVYSPLMQGGALNAIVDFFQALVVIKAVNMSYADLMKQLTSPVYSSTSSGVSTTLHKQAFHSVAKCVAALSSVCPKEIPGLVNLFIQDVKNPKSTTAVRMLAFLSLAEIGRTTNLSTQKELKAVILDAFASPSEEVKSAASYALGNISVGNLKEYLPFMLKEIGGQPKRQYLLLHSLKEVISSSPAESLKPYGEDIWALLFKHCEYTEEGTRNVVAECLGKLTVVNPSQLLPRLKKQLFSGSPYARSTVVTAIKFTISDHPQPIDPLLKGCIGDFLKTLQDPDLNVRRVALAMFNSAAHNKPSLIRDQLSTVLPHLYNETKVRRELIREVEMGPFKHTVDDGLDVRKAAFECMYTLLESCLDRLDIYEYLNHVEDGLKDHYDIRMLTFIMLARLSTLCPNAVLQRLERLVEPLRVTCSTKVKAGSVKQEFEKQDELKRSAMRAVAALLTIPDADKSPVMAEFSSQIRANPEMSSLFESIQKDPTSLSASESMELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMA1</accession>
    <name>A0A6I9XMA1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Serine/threonine-protein kinase Nek9</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Nercc1 kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Never in mitosis A-related kinase 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">NEK9</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013917019.1">
        <property type="nucleotide sequence ID" value="XM_014061544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMA1"/>
    <dbReference type="GeneID" id="106545084"/>
    <dbReference type="KEGG" id="tsr:106545084"/>
    <dbReference type="CTD" id="91754"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08221">
        <property type="entry name" value="STKc_Nek9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000118">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000602">
        <property type="entry name" value="serine/threonine-protein kinase Nek9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000021">
        <property type="entry name" value="serine/threonine-protein kinase Nek9 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042767">
        <property type="entry name" value="Nek9_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051997">
        <property type="entry name" value="STK_NEK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535">
        <property type="entry name" value="PROTEIN CBG16200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 28" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="42"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="24">
        <location>
            <begin position="378"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="24">
        <location>
            <begin position="435"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="24">
        <location>
            <begin position="489"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="24">
        <location>
            <begin position="541"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="24">
        <location>
            <begin position="606"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="24">
        <location>
            <begin position="659"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="733"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="918"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="744"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="925"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00067736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013917019.1"/>
        </source>
    </evidence>
    <sequence length="964" mass="106711" checksum="948D361068C5330C" modified="2020-10-07" version="1">MSLNQYERHYNSINSDFGSESSSGTSPRPGSGLGGEQEELHYAPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDTLFEEEMVVWYLFQIASAVSCIHREGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYSFKSDIWAVGCVAFELLTLKRTFDATNPLNLCVKIVQGNRAMEVDSSIYSVDLIQMVHSCLHQDPEKRPTADELLESPFIKKRRRDMEEKVALLNGPNKRPRTSTMNEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKNGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIRQVSCGDDFTICITGEGQAFAFGSDYYGCIGVDKAFGSEVLEPRQINFFSSNPVEQVSCGDNHVAVLTRNREVYTWGCGEYGRLGLDSEEDHSIPQKVEIQKTSHIVSVQCGSDGTFLLTQAGKVLACGLNEFNKLGLNQCTSGIINHDAYQEIPYATTFTLAKKLSFYKIRTIAPGKTHTAAIDERCRLLTFGSNKCGQLGVGDYKKHVGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERAHSSDICTSWPRPIFGSLHYVPDLSCRGWHTIIIVEKVINSKTILRSSSSGLSVGTVAQSSTAEPNEEEETEQDSESPDNSRGFRRTMEADCGMGDQISSTEVAVDSSAASSSCPSWLRKELENAEFIPMPQTPSLESLASSRSDKETFPYNELKGLHMPSPTPVDINESKSESWQLVLDSAESCDRGKLPVASSECKCSFLQAEVERLSGLISKCLADQEKLQQETARLNAQLQVLSRRQGTHQLESHSKATQTSKEEMESDPKPDLDSESWCLLGTDSCRFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMG0</accession>
    <name>A0A6I9XMG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Calpain-9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Digestive tract-specific calpain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">New calpain 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">CAPN9</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Calcium-regulated non-lysosomal thiol-protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915110.1">
        <property type="nucleotide sequence ID" value="XM_014059635.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915111.1">
        <property type="nucleotide sequence ID" value="XM_014059636.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915112.1">
        <property type="nucleotide sequence ID" value="XM_014059637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMG0"/>
    <dbReference type="GeneID" id="106543585"/>
    <dbReference type="KEGG" id="tsr:106543585"/>
    <dbReference type="CTD" id="10753"/>
    <dbReference type="OrthoDB" id="424753at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000151">
        <property type="entry name" value="Calpain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF385">
        <property type="entry name" value="CALPAIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="15">
        <location>
            <begin position="42"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="590"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="active site" evidence="12 13">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="12 13">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="active site" evidence="12 13">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013915110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013915111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013915112.1"/>
        </source>
    </evidence>
    <sequence length="706" mass="80711" checksum="6A59511AEA36A455" modified="2020-10-07" version="1">MPCTYRDPTARAPPMPQNASATHSSGKTYQELKLECQQKGILFEDCDFPANDSSLFYNEKPSIPFVWKRPGEIVKDPQFILGGATRTDICQGDLGDCWLLAAIASLTLNEKVLARVIPQNQDFDSDYAGIFHFQFWQYNEWLDVVIDDRLPTFKDRLVFLHSADLNEFWSALLEKAYAKLNGSYEALKGGSSIEAMEDFTGGVGETFEVKKAPKNFYALLQKALQRGSLVGCSIDISNAAESEARTPSGLIKGHAYSVTGIDEVNYQGQTVRLIRVRNPWGQVEWNGAWSDNSSEWDSLSPSEKQQLHHTALDDGEFWMKFEDFLSHFEKVEICNLTPDALEDNAAHKWEVSIHQGSWVRGATAGGCRNFIETFWTNPQFKLQLAEKDEGQDECTFVAALMQKNRRKLRKLGAALLTIGYAIYESPDKDEHLTKDFFRYHASKARSKTYINLREVSDRFELPPGDYIIVPTTYEPQQEADFCLRVFSEKRVVTKEMDGNVNIDLPEIPEPTQPQQETEEEKQFRDLFKQISGPDMEISAEELEYILNAVLEKNKIKFKKISLLSCKNIISLMASSGNEKLEFNEFKLFWDKLKKWITLYLHFDSDQSGTMSSHELRLALKAAGFQLNNYLLQLIVLRYSDDQQQIEFDDFLNCLIRLENASRVFQALCVENRDFINLHINEVGVYPQETSLRAVSCSVFGIYMTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XN40</accession>
    <name>A0A6I9XN40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Growth hormone-releasing hormone receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Growth hormone-releasing factor receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106541303</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912168.1">
        <property type="nucleotide sequence ID" value="XM_014056693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XN40"/>
    <dbReference type="GeneID" id="106541303"/>
    <dbReference type="KEGG" id="tsr:106541303"/>
    <dbReference type="OrthoDB" id="5967113at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016520">
        <property type="term" value="F:growth hormone-releasing hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004999">
        <property type="term" value="F:vasoactive intestinal polypeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000014">
        <property type="entry name" value="Growth hormone-releasing hormone receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000032">
        <property type="entry name" value="Vasoactive intestinal polypeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001571">
        <property type="entry name" value="GPCR_2_VIP_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002284">
        <property type="entry name" value="GPCR_2_VIP_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF22">
        <property type="entry name" value="VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00491">
        <property type="entry name" value="VASOACTVEIPR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01155">
        <property type="entry name" value="VIP2RECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026702288" description="Growth hormone-releasing hormone receptor" evidence="18">
        <location>
            <begin position="22"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="19">
        <location>
            <begin position="38"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="20">
        <location>
            <begin position="126"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013912168.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="49791" checksum="01198F0FC589B9D2" modified="2020-10-07" version="1">MQLSLLLLFSTCWWTLGPVKSIHPECRMQQEIYQEEKKCMELLKNANTVSLTGCVGVWDNITCWRSAEIGETVTVPCPKIFSKFFNKQGNISKNCTSTGWSEIFPHFFVACGFEDIEDNPVFYVWVKAIYTLGHSVSLIALITGSIILCVFRKLHCTRNYIHLNLFLSFILKAIAVLVKDGILYSDTSPAKCSDHASWVGCKASLVFFQYCVMANFYWLLVEGLYLHILLMIMFSSNRHFSAYLLTGWGIPTIFIIIWIITRVYLDNNGCWDTNDHSAPWWIIRVPILISILVNFVIFISIIRILLQKLRSPDVGGNEQSQYKRLAKSTLLLIPLFGVHYVVFAVFGVSKKYQIVFELCLGSFQGLVVAILYCFLNSEVRGELKRKWKSWCLNERLSRDYKVHRSSVFPNDSEGGPQLHRNSATQFSSQTETSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQL3</accession>
    <name>A0A6I9XQL3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Forkhead box protein B2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FOXB2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916342.1">
        <property type="nucleotide sequence ID" value="XM_014060867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQL3"/>
    <dbReference type="GeneID" id="106544567"/>
    <dbReference type="KEGG" id="tsr:106544567"/>
    <dbReference type="CTD" id="442425"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20043">
        <property type="entry name" value="FH_FOXB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000082">
        <property type="entry name" value="forkhead box protein B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047389">
        <property type="entry name" value="FOXB1_B2_FH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF215">
        <property type="entry name" value="FORKHEAD BOX PROTEIN B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013916342.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="36653" checksum="185009F6F817D6D1" modified="2020-10-07" version="1">MPRPGKSSYSDQKPPYSYISLTAMAIQHSSEKMLPLSDIYKFIMERFPYYREHTQRWQNSLRHNLSFNDCFIKIPRRPDQPGKGSFWALHPDCGDMFENGSFLRRRKRFKVPRPEPHMPAAAGKAGVLHPHLSHYFHPHHPPPPPPPPLQPPAATKGHPDSAAAVAAAVGRLPPFQPYSPAPGFKHPFAIENLIGRDYKGVIQGSGLPLASVMHHLGYPMAGQQISTMVSSVWPHVGMLEPAGGIPLSPREYGPFGMPVKTICHPSTQTLPAIPVPIKPTPSIAPSTAISALTVNHVTQQLCQTTSPSVSSSLLEQIPPNSSNGKISLHSALVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQZ6</accession>
    <name>A0A6I9XQZ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cysteine protease</fullName>
            <ecNumber evidence="19">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ATG4B</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylserine + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phospho-L-serine</text>
            <dbReference type="Rhea" id="RHEA:67576"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="Rhea" id="RHEA-COMP:17326"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172942"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:67577"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013916196.1">
        <property type="nucleotide sequence ID" value="XM_014060721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQZ6"/>
    <dbReference type="GeneID" id="106544451"/>
    <dbReference type="KEGG" id="tsr:106544451"/>
    <dbReference type="CTD" id="23192"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046793">
        <property type="entry name" value="ATG4_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF39">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20166">
        <property type="entry name" value="ATG4_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 19" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 19 22" id="KW-0645">Protease</keyword>
    <keyword evidence="13 19" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="20">
        <location>
            <begin position="39"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013916196.1"/>
        </source>
    </evidence>
    <sequence length="393" mass="44437" checksum="D360685E54E2C8A5" modified="2020-10-07" version="1">MDAATLTYDTLRFEYEDFPETKEPVWILGRKYNALTEKEDILLDVTSRLWFTYRKGFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVGRHLSRDWRWMKGKKQTDNYYNVLNAFIDKKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLATFDMWSSLAVHIAMDNTVVIEEIRRLCKPNCPCSGASAFPSAESDFLSNGYPDGDVSTDSLLLWKPLVLLIPLRLGLTDINEAYIETLKRCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVDPVDSCHISDESFHCQHPPCRMSIAELDPSIAVGFFCKKEEDFSDWCQQIKKLSLIRGALPMFELIEHQPSHFSSPDVLNLTPDSSDADRLERFFDSEDEDFEILSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSN9</accession>
    <name>A0A6I9XSN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(+) diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NUDT12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60876"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:60877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. NudC subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013919079.1">
        <property type="nucleotide sequence ID" value="XM_014063604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSN9"/>
    <dbReference type="GeneID" id="106546675"/>
    <dbReference type="KEGG" id="tsr:106546675"/>
    <dbReference type="CTD" id="83594"/>
    <dbReference type="OrthoDB" id="10249612at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919079.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="15617" checksum="06EEDD93F6589EF9" modified="2020-10-07" version="1">MINAHSTSSSGHWLLKSLGYSSIMSDLSSKADLEISSTGETIENAVRREVEEESGVKVGRVEYVSCQPWPMPSSLMIGCIAAAVSTEIKVDNIEIEDARWFSREQIIEILNKKNQNIFVPPYQAIAHQLIKHWIGMNANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSU1</accession>
    <name>A0A6I9XSU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC106546716</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
            <location evidence="5">Centromere</location>
            <location evidence="5">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19 20">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919134.1">
        <property type="nucleotide sequence ID" value="XM_014063659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSU1"/>
    <dbReference type="GeneID" id="106546716"/>
    <dbReference type="KEGG" id="tsr:106546716"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000162">
        <property type="entry name" value="septin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008115">
        <property type="entry name" value="Septin7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01742">
        <property type="entry name" value="SEPTIN7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14 20" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="22">
        <location>
            <begin position="45"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="389"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="389"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013919134.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="51426" checksum="E4286E96842E655E" modified="2020-10-07" version="1">MRSTQSGCYSLTMRIGVSVAQQKNLEGYVGFANLPNQVYRKSVKRGFEFTLMVVGESGLGKSTLINSLFLTDLYSPEYPGPSHRIKKTVQVEQSKVLIKEGGVQLLLTIVDTPGFGDAVDNSNCWQPVIDYIDSKFEDYLNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQIMKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCDFTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKFDTVEGMSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEDEKSNWETQQRILEQQNTSRTLEKNKKKGKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTP5</accession>
    <name>A0A6I9XTP5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RUN and FYVE domain-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RUFY2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914068.1">
        <property type="nucleotide sequence ID" value="XM_014058593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTP5"/>
    <dbReference type="GeneID" id="106542772"/>
    <dbReference type="KEGG" id="tsr:106542772"/>
    <dbReference type="CTD" id="55680"/>
    <dbReference type="OrthoDB" id="79871at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17695">
        <property type="entry name" value="RUN_RUFY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000001">
        <property type="entry name" value="RUN and FYVE domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000046">
        <property type="entry name" value="RUN and FYVE domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047335">
        <property type="entry name" value="RUFY1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047332">
        <property type="entry name" value="RUN_RUFY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45956:SF3">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45956">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RUN" evidence="8">
        <location>
            <begin position="69"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="572"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="259"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="330"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="526"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914068.1"/>
        </source>
    </evidence>
    <sequence length="638" mass="73333" checksum="D791EEFEBB203B4C" modified="2020-10-07" version="1">MGDEEGWKRAVKAAKAGSALNCSGSCCSSPLTMAVKDPQAIERTNLLNMAKLSIKGLIESALSFGRTLDSDYPPLQQFFVVMEHCLKHGLKVKKSFLSYNKTIWGPLELVEKLYPEAEEIAASVRDLPGLKTSLGRARAWLRLALMQKKMADYLRCLILRKDLLGEFYEYHAVMMEEEGAVIVGLLVGLNVIDANLCIKGEDLDSQVGVIDFSMYLKSDDDTEHKERNDHVAAILDQKNYVEELNRQLNSTVGSLHTRVDSLEKSNTKLIEELAIAKNNIIKLQEENHHLRSENTLILMKTQHHLEMNKVDAEAELQTYKHSRQSLDEMYNEAHRQLREESQLRQDIENELMVQVGMKHEIELAMKLLEKDIHEKQDTLINLRQQLEEVKVINTEMYQKLQFSDEAMKEKNEIIGRLEDKTSEITATMKHLEQRLQQAEKAHMEADSEDKKVKQDYMNKSESLQKEIAQKENQVCQLETDLKIEKEWRQTMEEDLKKEKETTCFLQTETQQIITLKKEFLKLQGNNKQLRNLCHDQEEALQELAGKLSESKLKIEDIKEANKTLQGLVWLKDKEATHCKLCEKEFSLSKRKHHCRNCGEIFCNACSDNELPLPSSPKPVRVCDSCHALLIQRCSSNVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTP9</accession>
    <name>A0A6I9XTP9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ryanodine receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106542775</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914073.1">
        <property type="nucleotide sequence ID" value="XM_014058598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTP9"/>
    <dbReference type="GeneID" id="106542775"/>
    <dbReference type="KEGG" id="tsr:106542775"/>
    <dbReference type="OrthoDB" id="9048498at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005790">
        <property type="term" value="C:smooth endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005219">
        <property type="term" value="F:ryanodine-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014808">
        <property type="term" value="P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006941">
        <property type="term" value="P:striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000040">
        <property type="entry name" value="Ryanodine receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009460">
        <property type="entry name" value="Ryanrecept_TM4-6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399">
        <property type="entry name" value="B30.2/SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399:SF10">
        <property type="entry name" value="RYANODINE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06459">
        <property type="entry name" value="RR_TM4-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="875"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="Ryanodine Receptor TM 4-6" evidence="3">
        <location>
            <begin position="928"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="930"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="943"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914073.1"/>
        </source>
    </evidence>
    <sequence length="1087" mass="124325" checksum="5F91C23EB18C64A7" modified="2020-10-07" version="1">MLDDVQVSCYRTLSSIYSLGTTKNPYVERQRPQLGECLARLAAAMPVAFLEPHINEFNPCSVYTTKSPRERAILGLPNCVEEMCPDIPVLEKLMKDINGLAESGARYTEMPHVIEVTLPMLCNYLPRWWERGPETNAQGPWCTEVTSNQLNTLLGNILKIIVSNLGIDEASWMKRLAVFAQPIVSKAKPELLRSHFIPTMEKLKKRSGKVVAEEEQLRLEAKSESEDTELLIRDEFSVLCRDLYALYPLLIRYVDNNRAHWLTEPNSDAEELFRMIGEIFIYWSKSHNFKREEQNFVVQNEINNMSFLTADSKSKMSKSGGSDQERHKKKRRGDRYSVHTSLIVATLKKMLPIGLNMCSPNDQELITLAKLRYALKDTDEEVREYLNNNLHLQGKCDNSCSMRWQMALYKEMAGKAEDCNKPEKIVKRVQEVSAVLYHLEQLEHPYKSKKAAWHKLLSKQRRRAVVACFRMTPLYNLPRHRACNMFLESYKYSWIFSEEHPFEDRMIDDLSKPGPQEEEEEEEGPCTGNQQSLAHSRLWDAVIGFLHVFAHMMMKLAQDPGQHNFLVFLQSRCQNIICILGCIYSGNVVHGTIARQMVDMLVESSSNVEMILKFFDMFLKLKDIVASDAFRDYITDPRGLISKKDFQKAMDSQKQYTPSEIQFLLSCSEADENEMIDYEEFANRFQEPAKDIGFNIAVLLTNLSEHMPHDARLKTFLELAECILNYFNPYLGRIEIMGASKRIERIYFEISEANKTQWEMPQVKESKRQFIFDVVNEGAESEKMELFINFCEDTIFEMQIASQISEEEGEAVEEEEEEAESTESGESESASAPSEASSVFGEFLESILNFFHLFTYRNIRRQFRKVKKMTVKEMAVGVATFLYTILMGILVFFYSVLKGFFLLIWNTLFGGGLVEEAKKLTMTELLASMPDPTQDEVHGDVAPSDEEEQATEEGEEISIVEPGKEEATVEGGEGYGADPKKEHFRSTAADAPGGLGDMGDTTPVEPPTPEGTPILKRKLVEGEAAEDESYMEMEKIEEPPAEPEKADAENGEKAAKEEEKKKEEEKEHKEEEPPSPPPAKEEKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTZ4</accession>
    <name>A0A6I9XTZ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">KPNA1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917527.1">
        <property type="nucleotide sequence ID" value="XM_014062052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTZ4"/>
    <dbReference type="GeneID" id="106545474"/>
    <dbReference type="KEGG" id="tsr:106545474"/>
    <dbReference type="CTD" id="3836"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000001">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000013">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="126"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013917527.1"/>
        </source>
    </evidence>
    <sequence length="538" mass="60339" checksum="4553C72C90621B58" modified="2020-10-07" version="1">MTSSGKENFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATADEETEEEVMSDGGFHETQMNNMEMTSNAVITSDMTEMIFSNSPEQQLSATQKFRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKENCTLQFEAAWVLTNIASGNSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDCNILPPLLQLLSKQNRLTMTRNAVWALSNLCRGKNPPPDFVKVSPCLSVLSWLLFVNDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVISPALRAVGNIVTGDDIQTQVILNCAALQSLLHLLSSPKESINKEACWTISNITAGNRAQIQTVIDANIFPALINILQTAEFRTRKEAAWAITNATSGGSAEQIKYLVELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQEARRSGTGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTEEEDSNIVPQVDIGQQQYIFQQCEAPMEGFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUD6</accession>
    <name>A0A6I9XUD6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">UDP-glucose ceramide glucosyltransferase-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">UGGT1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + UDP-alpha-D-glucose = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61304"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:59080"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919719.1">
        <property type="nucleotide sequence ID" value="XM_014064244.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547175"/>
    <dbReference type="KEGG" id="tsr:106547175"/>
    <dbReference type="CTD" id="56886"/>
    <dbReference type="OrthoDB" id="27683at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003980">
        <property type="term" value="F:UDP-glucose:glycoprotein glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06432">
        <property type="entry name" value="GT8_HUGT1_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000004">
        <property type="entry name" value="UDP-glucose glycoprotein glucosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040497">
        <property type="entry name" value="Glyco_transf_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009448">
        <property type="entry name" value="UDP-g_GGtrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040693">
        <property type="entry name" value="UGGT_TRXL_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040694">
        <property type="entry name" value="UGGT_TRXL_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040692">
        <property type="entry name" value="UGGT_TRXL_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040525">
        <property type="entry name" value="UGGT_TRXL_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226">
        <property type="entry name" value="UDP-GLUCOSE GLYCOPROTEIN:GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226:SF3">
        <property type="entry name" value="UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18404">
        <property type="entry name" value="Glyco_transf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18400">
        <property type="entry name" value="Thioredoxin_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18401">
        <property type="entry name" value="Thioredoxin_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18402">
        <property type="entry name" value="Thioredoxin_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18403">
        <property type="entry name" value="Thioredoxin_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06427">
        <property type="entry name" value="UDP-g_GGTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026879319" description="UDP-glucose ceramide glucosyltransferase-like 1" evidence="13">
        <location>
            <begin position="20"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="14">
        <location>
            <begin position="34"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="15">
        <location>
            <begin position="289"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="16">
        <location>
            <begin position="428"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="UDP-glucose:glycoprotein glucosyltransferase thioredoxin-like" evidence="17">
        <location>
            <begin position="700"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosyltransferase 24 catalytic" evidence="18">
        <location>
            <begin position="1234"/>
            <end position="1501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013919719.1"/>
        </source>
    </evidence>
    <sequence length="1534" mass="174550" checksum="773DC882DBD5EE88" modified="2020-10-07" version="1">MGTVTALIGIWMTFSLAEAHSKAVTTSLTTKWFSTPLLLEASEFLAEEDNEKFWNFVETCQNFQSSNHDGTDYSSYNAVLQAASQNLSPLQQNLLKFVLSLRSYSATVQAFQQIAADEPAPEQCNAFFVVHGEKTCEADRLGTLIQTASKRPKPFMFKGDHKYPGSNSESPIVIMYAEIGSKQFSEFHRLLVSKAIAGEINYILRHYVANPNKEKVYLSGYGVELAIKSTEYKAKDDTQVKGTDINSTVIGDSDPIDEVQGFLFGKLRQSHPHLEEELKELRKHLVESTNEMAPLKVWQLQDLSFQTAARILAAPTMDALTVMKDLSQNFPTKARAITKTVVPSELRSEIEENQKYFKGTLGLQLGDSALFINGLHIDLDTQDIFSLFDVLRNEARVMEGLHSLGIVGHSMHNVLKLNIQPSDSDYAVDIRNSAISWINNLEIDSKYNSWPSSVQELLRPTFPGVIRQIRKNFHNFVLLLNPSHESSAELLGVAEMFLSNHIPLRVGLVFVVDNSEDVDGLQDAGVALLRAYNYVAQEVDTNYAFQTIISMYNKVNAGEKLKVEHVISVLEKQYPYVEVNSILGIDSAYDQNRKEGYTYYEQTGVGPLPIVLFNGMPLQKEQLDPDELETVTMHKILETTSYFQRAVYLGELSNDQDVVEYIMTQPNVVPRINSRILMSDREYLDLTANNNFYVDDYARFSTLDSKDKTAAIANSMTYLTKKGMSSKEIYDDTFVRPVTFWIVGDFDKPSGRQLLYDAIKHQKSSNFIRIGVINNPSEDPAAKNTIIARAIWAALQTQGSNNAKNFITKLLKEENAKMLESGTDITEFAVGGMEISTFKAAFEAPEVGFILSHAVYCRDVLKLKKGQKAVISNGRIIGPLEAGELFNQDDFHLLENIIVKTSGQKIKSHVQQLGVEEDLASDLVMKVDALLSAQPKGEARIEYQFFEDKYSAIKLRPKEGEIYFDVVAIVDPATREAQRLAPLLMVLNKLINMNLRVFMNCQSKLSDMPLKSFYRYVLEPEITFTAEKHLIPGPVAKFLDMPQSPLFTLNLNTPESWMVESVRASYDLDNIYLEEVESVVAAEYELEHLLLEGHCYDITTGQPPRGLQFTLGTSTNPVIVDTIVMANLGYFQLKANPGAWFLRLRNGRSDDIYKIYSHDETDSPPDASDVVVVINNFKSKIIKVKVQKKPDMLNEDLLSDGTNENESGFWESLKWGFTGGQKKEEVKQDKEDVINIFSVASGHLYERFLRIMMLSVLKHTETPVKFWFLKNYLSPTVKEFIPYMAKKYNFQYEFVQYKWPRWLHQQTEKQRIIWGYKILFLDVLFPLAVDKFLFVDADQIVRTDLKDLRDFNLDGAPYGYTPFCESRREMDGYRFWKSGYWASHLAGRKYHISALYVVDLKKFRKIAAGDRLRGQYQGLSQDPNSLSNLDQDLPNNMIHQVPIKSLPQEWLWCETWCDDASKKRAKTIDLCNNPMTKEPKLQAAMRIVPEWQDYDQEIKQLYNQFHQEKESGILITTNETESQSQKDTLLHGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUT5</accession>
    <name>A0A6I9XUT5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Disintegrin and metalloproteinase domain-containing protein 17-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106545709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917827.1">
        <property type="nucleotide sequence ID" value="XM_014062352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUT5"/>
    <dbReference type="GeneID" id="106545709"/>
    <dbReference type="KEGG" id="tsr:106545709"/>
    <dbReference type="OrthoDB" id="2131567at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013917827.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15325" checksum="09FB5E7C6321E4EB" modified="2020-10-07" version="1">MDKKLDRQYEENTKSLFGPSNAEMLSSMDSASIRIIKPFPVVPPASRHQPLQTIVPVSVAAAPPKLDHQRMDTIQEDPSTDSHMEEDCFEKDPFPNNSTAAKSFEDLTEHPVTRSEKNSFKLQRQHRVDSKETEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV25</accession>
    <name>A0A6I9XV25_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Survival of motor neuron-related-splicing factor 30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Survival motor neuron domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SMNDC1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in spliceosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917636.1">
        <property type="nucleotide sequence ID" value="XM_014062161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV25"/>
    <dbReference type="GeneID" id="106545556"/>
    <dbReference type="KEGG" id="tsr:106545556"/>
    <dbReference type="CTD" id="10285"/>
    <dbReference type="OrthoDB" id="79171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20399">
        <property type="entry name" value="Tudor_SPF30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000038">
        <property type="entry name" value="Survival of motor neuron-related-splicing factor 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010304">
        <property type="entry name" value="SMN_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13681:SF26">
        <property type="entry name" value="SURVIVAL OF MOTOR NEURON-RELATED-SPLICING FACTOR 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13681">
        <property type="entry name" value="SURVIVAL OF MOTOR NEURON-RELATED-SPLICING FACTOR 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06003">
        <property type="entry name" value="SMN_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Tudor" evidence="12">
        <location>
            <begin position="72"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013917636.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="26721" checksum="8FD3764D98861B38" modified="2020-10-07" version="1">MSEDLAKQLASYKAQLQQVEAALTGNGDNEDLLKLKKDLQEVIELTKDLLSTQPSETLANSDSSASALPSHTWKVGDRCMAIWSEDGQCYEAEIEEIDEENGTAAITFSGYGNAEVTPLLNLKPVEEGRKTKEDSGNKPMSKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQFNNRAYSKNKKGQVKRSIFASPESVTGKVGVGTCGIADKPMTQYQDTSKYNVRHLMPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW62</accession>
    <name>A0A6I9XW62_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kelch domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">KLHDC4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918382.1">
        <property type="nucleotide sequence ID" value="XM_014062907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW62"/>
    <dbReference type="GeneID" id="106546126"/>
    <dbReference type="KEGG" id="tsr:106546126"/>
    <dbReference type="CTD" id="54758"/>
    <dbReference type="OrthoDB" id="4447at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052588">
        <property type="entry name" value="Kelch_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013918382.1"/>
        </source>
    </evidence>
    <sequence length="573" mass="63659" checksum="51B53F6FA9973157" modified="2020-10-07" version="1">MGKKNKKEKKAKGAEKTAAKMEKKVSKRTKKEEEDLEVLIAQFQSLDAAKTQVLETPCAPPSPRLNASLSAHPEKDELILFGGEYFNGQKTLLYNELFVYNIRKDSWAKVDIPSPPPRRCAHQAVVVAQGGGQLWLFGGEFASPDGEQFYHYRDLWVLHLANRTWEQVKASGGPSGRSGHRMAVSKRQLIVFGGFHESSRDYIYYNDVHAFNLDTFAWSKLAPSGIAPAPRSGCHLASTPEGHLVVYGGYSKQRIKKDVDKGTLHADMFLLSAEGLEKWSWSRVSPSGVKPTPRSGFSVAMGPGNRSLLFGGVHDEEEEERLEGDFFNDIYFYDLAKNRWFLGQLKGSKSEKKRRRRGAAKGEPHQAGGGGDEAEGQPPRGPVEIVKEVVAEDGTVMIVKQLISAPQGKSTESPESEGEDEDVGNGASNPPVEPCPRSNAMLAVKHGVLYLYGGMFEVGDRQFTLNDLYALDLHKMEEWKVLVEMDPKTQEWLEESESDEDVEGAEGGEEEEEEEEIPGESAPDSNRFRTAWDDYYVEPGYYDDSITQTTITTTTQTTEEDVKVTPTPRPTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX13</accession>
    <name>A0A6I9XX13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar protein 56</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nucleolar protein 5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NOP56</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the early to middle stages of 60S ribosomal subunit biogenesis. Required for the biogenesis of box C/D snoRNAs such U3, U8 and U14 snoRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with TCOF1 in an RNA-independent manner. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles; the core proteins SNU13, NOP56, NOP58 and FBL or FBLL1 assemble stepwise onto the snoRNA. Interacts with NOP1 and NOP58. Interacts with NUFIP1, RUVBL1 and RUVBL2; RUVBL1:RUVBL2 seem to bridge the association of NOP56 with NUFIP1. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with NOP2 and FBL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909035.1">
        <property type="nucleotide sequence ID" value="XM_014053560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XX13"/>
    <dbReference type="GeneID" id="106538919"/>
    <dbReference type="KEGG" id="tsr:106538919"/>
    <dbReference type="CTD" id="10528"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000001">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="9"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="159"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909035.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29022" checksum="5E08A7BCC97C0F67" modified="2020-10-07" version="1">MGGHTNRMDGMNRFSVLSAQVGARLISHAGSLTNLAKYPASTVQILGAEKALFRALKTRGNTPKYGLIFHSTFIGRAAAKNKGRISRYLANKCTIASRIDCFSEVPTAVFGDKLREQVEERLAFYETGEVPRKNLEVMKEAVEEASEAAAEIKRKLQKKERKKLKQEKKRLQALAAAAETQNNNSSPLLEPPEQENQAKAKRKKRKPQELEAEPANNSLGDEDAPLPKKKKKPPAVVVVEEPPAPQGKKAKKRKARADEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYL3</accession>
    <name>A0A6I9XYL3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrator complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">INTS7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Integrator subunit 7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919302.1">
        <property type="nucleotide sequence ID" value="XM_014063827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYL3"/>
    <dbReference type="GeneID" id="106546855"/>
    <dbReference type="KEGG" id="tsr:106546855"/>
    <dbReference type="CTD" id="25896"/>
    <dbReference type="OrthoDB" id="1921953at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033060">
        <property type="entry name" value="INTS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054519">
        <property type="entry name" value="INTS7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056517">
        <property type="entry name" value="INTS7_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056516">
        <property type="entry name" value="INTS7_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13322">
        <property type="entry name" value="C1ORF73 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13322:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22965">
        <property type="entry name" value="INTS7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24437">
        <property type="entry name" value="INTS7_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24436">
        <property type="entry name" value="INTS7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 7 N-terminal" evidence="9">
        <location>
            <begin position="25"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 7 helical bundle" evidence="10">
        <location>
            <begin position="538"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 7 C-terminal" evidence="8">
        <location>
            <begin position="804"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="941"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="941"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919302.1"/>
        </source>
    </evidence>
    <sequence length="966" mass="107055" checksum="574EAE484AA6C9AB" modified="2020-10-07" version="1">MASVSKSFLADAGYGEQELDANSALMELDKGLRSGKLGEQCEAVVRFPRLFQKYPFPILINSAFLKLADVFRVGNNFLRLCVLKVTQQSEKHLEKILNVDEFVKRIFSVIHSNDPVARAITLRMLGSMASIIPERKNAHHSIRQSLDSHDNVEVEAAIFAAANFSAQSKDFAGGICNKISEMIQGLVTPVDLKLKLIPILQHMHHDASLASSARQLLQQLVTSYPSTKMVIVTLHTFTLLAASSLVDIPKQIELLLQYMKNDRREAVKRLAIQDLKLLAKKTPHTWNNENIETLCECALQTPYDSLKLGMLSVLSTLSDTIAIKQYFSIAPGTKSTTARSSNLVKLAQESCYHNNQNIAAHGVRVLTNIAASCQEKELLPLEQDAVFGLESLLVLCSQEPSSSPEATLKIVLTCMVKLAKSHPHLSQSIVESLLTQLPRAQDAARILICHCLAAIAMQLPVLGDGMLGDLMNLYKVIGCSAANKQQELLVSLATVIFVASQKTLSSEVKAVIKQQLENDSNGWTAYRIARQASRMGNHGMAKELYQSLLTQVASEHFYFWLNSLKEFSHAEQCLTGLQEDDYSSALSCIAESLKSYHKGIASLTAASTPLNPLSFQCGFVKLRIDLLQAFSQLICTCNSLKTSPPPAIATTIAMTSGSDLQRCGRISNQMKLSMEEFRNLAARYSDLYQSSFDADSETLRNVELQQQSCLLISHAIEALILDPESASFQEYGSTGVAHAASEYERRMMSVFGHVLEEVEALNQKYAPVSYMHTACLCSAVIALLKVPLSFQRYFFQKLQSTSIKLALSPSPRNPAEPIVVQNNQQLALKIEGVVQHGSKPGLFRKIQSICLNVSSTLQSKPGQEYKIPIDNLTNEMEQMVEPHNDYFSTQFLLNFIVVGTHSITVESSVRDLNGIIWKTGPKTTLLIKSLEDPYSQQIRLQQQQGQQLSQQPQQLQPQPRTAYSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ23</accession>
    <name>A0A6I9XZ23_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase 1 domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GLT1D1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013909740.1">
        <property type="nucleotide sequence ID" value="XM_014054265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ23"/>
    <dbReference type="GeneID" id="106539465"/>
    <dbReference type="KEGG" id="tsr:106539465"/>
    <dbReference type="CTD" id="144423"/>
    <dbReference type="OrthoDB" id="512920at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052622">
        <property type="entry name" value="Glycosyltransferase_G1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46660:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013909740.1"/>
        </source>
    </evidence>
    <sequence length="266" mass="29683" checksum="4F9F0206226E5C4B" modified="2020-10-07" version="1">ILYPPGGGPVARLGSPGRIQDHLQAAGHLCILKDTSDYESPLAISKLISSEKLEAALGIHLFKAGRLLQGSPVPFGIIFGGTDVNEDPKSEEKFQIMEKVLDEARFAVSFTEAMKEAAATYWVGIVSLPNRQHNWAEFLQRAGKNAYKREMSQNFYTSVICEASVLPDVSPVFSVLQAMDLNVLVLARDIPGNAAVIAHQTTGLLYTTPKEFVQLAKTLMKDPVLYREIVRKAHEYVLKYHSWEKERKVYQNLVLRLHECSCTNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ31</accession>
    <name>A0A6I9XZ31_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106539476</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909750.1">
        <property type="nucleotide sequence ID" value="XM_014054275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ31"/>
    <dbReference type="GeneID" id="106539476"/>
    <dbReference type="KEGG" id="tsr:106539476"/>
    <dbReference type="OrthoDB" id="9902777at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF227">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909750.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35653" checksum="F3CA147796FD38F3" modified="2020-10-07" version="1">MEFLNSSTITAFVLLGFRVEQQGRLLLLSSFTLVYTLTLVENMVIILVVSQDAHLSSLPMYILLSNFSWLEVCYVSATVPRMLFDLSFPGGVISFHACFIQFYVFFSLGTTECFFLSAMALDRYLAICYPLRYTQIMSKKMCFFLVTLCWVIGFLWYLVPVFLISRLSFCGPNSIDHYICDSGGLLALACHPLETLSLFCYVITSALILGTFLFIAVSYVGIIFTLIKKSSENTRNKGVYTVSSHLAVVTLFYGSVATMYIGHSEKNRTENIKVMTLFYSAVAPLLNPLIYCLRNNQVKEALVRVLDLSLAQYEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZF2</accession>
    <name>A0A6I9XZF2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA decay activator protein ZFP36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Zinc finger protein 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZFP36L1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates with the cytoplasmic CCR4-NOT deadenylase complex to trigger ARE-containing mRNA deadenylation and decay processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909885.1">
        <property type="nucleotide sequence ID" value="XM_014054410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZF2"/>
    <dbReference type="GeneID" id="106539600"/>
    <dbReference type="KEGG" id="tsr:106539600"/>
    <dbReference type="CTD" id="677"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900153">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007635">
        <property type="entry name" value="Tis11B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF53">
        <property type="entry name" value="MRNA DECAY ACTIVATOR PROTEIN ZFP36L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04553">
        <property type="entry name" value="Tis11B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="271"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909885.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="36493" checksum="BFDE8C5C85B3AF6B" modified="2020-10-07" version="1">MSTALVSPTIFDLSEVLCKSNKMLNYGPSAAGGCLLDRKAVGTPAGGGFPRRHSVTLPNSKFHQTHLLNGLKGEPGPALGPRENRFRDRSFSEGGERLLQQKQPGGQANSSRYKTELCRPFEENGTCKYGDKCQFAHGIHELRNLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNAEERRAVAGGGREPVMAERPRLQHSFSFAGFPSAIAANGLLDSPTSITPPPIISADDLLVSPTLPDCASNPFTFSSQELTNLFVPSMGMQVPGGGSPTAFLLRPMSESPNMFDSPPSPQGSLSDQEGYLSSSSSSHSGSDSPILDNSRRLPIFSRLSISDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZX1</accession>
    <name>A0A6I9XZX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NFIC</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Binds DNA as a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CTF/NF-I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920007.1">
        <property type="nucleotide sequence ID" value="XM_014064532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZX1"/>
    <dbReference type="GeneID" id="106547389"/>
    <dbReference type="KEGG" id="tsr:106547389"/>
    <dbReference type="CTD" id="4782"/>
    <dbReference type="OrthoDB" id="10055441at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000647">
        <property type="entry name" value="CTF/NFI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020604">
        <property type="entry name" value="CTF/NFI_DNA-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019739">
        <property type="entry name" value="CTF/NFI_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019548">
        <property type="entry name" value="CTF/NFI_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492:SF2">
        <property type="entry name" value="NUCLEAR FACTOR 1 C-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492">
        <property type="entry name" value="NUCLEAR FACTOR I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00859">
        <property type="entry name" value="CTF_NFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10524">
        <property type="entry name" value="NfI_DNAbd_pre-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00349">
        <property type="entry name" value="CTF_NFI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51080">
        <property type="entry name" value="CTF_NFI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0010">Activator</keyword>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CTF/NF-I" evidence="11">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="260"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="447"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="454"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013920007.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="55983" checksum="26254FAFE0705A8B" modified="2020-10-07" version="1">MLTGAFPSVDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMTKDEERAVKDELLNEKPEVKQKWASRLLAKLRKDIRPEYREDFVLSITGKKPSCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKATQCTNPVLCVQPHHISVSVKELDLYLAYFVRERDSDQSSSPRTGIGSDQEDSKPIIMLDAADFQENFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPSSTGMRRTLPSTSSSGSKRHKSGSMEDDLDTSPGGEYYASPSSPTSSSRNWTEDMEGSISPTVKKTEMDKSPFNSPSPQDTSPRLFTQHHRPVIAVHSGISRSPHPSSALHFPTTSILPQAASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPSNQQPGPLNGGGQVKVPGHYLPTQMLAPPPPGMPRLAISPDTKSTTTTSEGGTTSPTSPTYSTPGTPPANRFVGLGPRDPGSIYQAQSWYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0I4</accession>
    <name>A0A6I9Y0I4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mothers against decapentaplegic homolog 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106547616</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920317.1">
        <property type="nucleotide sequence ID" value="XM_014064842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0I4"/>
    <dbReference type="GeneID" id="106547616"/>
    <dbReference type="KEGG" id="tsr:106547616"/>
    <dbReference type="OrthoDB" id="5794312at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10985">
        <property type="entry name" value="MH2_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF53">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH2" evidence="10">
        <location>
            <begin position="37"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013920317.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="25677" checksum="FA21D3DED4813415" modified="2020-10-07" version="1">MNHSLDAGSPNLSPNPMSPAHSNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y140</accession>
    <name>A0A6I9Y140_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="6 20">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC106547864</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + H2O = 1D-myo-inositol 1-phosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58433"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:43485"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="6 20"/>
    <dbReference type="RefSeq" id="XP_013920602.1">
        <property type="nucleotide sequence ID" value="XM_014065127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y140"/>
    <dbReference type="GeneID" id="106547864"/>
    <dbReference type="KEGG" id="tsr:106547864"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13363">
        <property type="entry name" value="PH_PLC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08558">
        <property type="entry name" value="PI-PLCc_eukaryota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000084">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000088">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF31">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE DELTA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 20" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="21">
        <location>
            <begin position="17"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="171"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013920602.1"/>
        </source>
    </evidence>
    <sequence length="395" mass="46243" checksum="2B3A82A8DDC37175" modified="2020-10-07" version="1">MSSLLCNHRMPLDDNLIQMQQGTMMRKVKSKNWKKQRFFMLQQDCMTVWYKSKMSGKAKSAFSVSDVETIREGHQSEVLRSLAEEFPAECCFTVVFHGRRSNQDLISSSAEEAQCWIQGLRRLIEMVINMDQKERLDQWICDWFLKADKDNDGRMNFKEVQHLLRMMNVNMNEDHAFQLFQMADKSETGSLEGEEFVLFYKALTQRDEVLEIFQEYSEDGKKLTLLEFVDFLQQEQMEVDGTDELAMELIDRYEPSETGKARHILSPDGFLTYLCSPDGSIFNPEHRKIFQDMSQPLCHYFISSSHNTYLIEDQLRGQSSVEGYIRALKQGCRCLEVDCWDGSNGEPIVYHGYTFTSKIPFRDVVTTLEKYAFWTFHYPLDNIFSFIFSSIEDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1Z2</accession>
    <name>A0A6I9Y1Z2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ethanolamine kinase</fullName>
            <ecNumber evidence="12">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ETNK1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013920511.1">
        <property type="nucleotide sequence ID" value="XM_014065036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1Z2"/>
    <dbReference type="GeneID" id="106547780"/>
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    <dbReference type="CTD" id="55500"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1200.10:FF:000002">
        <property type="entry name" value="Ethanolamine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF91">
        <property type="entry name" value="ETHANOLAMINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013920511.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="42768" checksum="16F69DC2C38795E9" modified="2020-10-07" version="1">MANYIHVPSGSPEVPKLDIFVSERDPSGGGNPGYRYGALELLRRLRPGWKPEEVTLKIFTDGITNKLIGCYVDEKMDDVVLVRIYGNKTELLVDREEEVKSFRVLQAHGCAPQLYCTFSNGLCYEFMQGEALDPEHVCHPEIFRLIARQLAKIHAIHAHNGWIPKSNLWIKMGKYFSLTPTEFADEELHKRFLRDVPSRQILQDEMAWMKERLSNLGSPVVLCHNDLLCKNIIYNGKQGDVQFIDYEYCGYNYLAYDIGNHFNEFAGVSEVDYNLYPSRKLQRQWLRSYLEAYKECKGFGRDVTEKEVEVLYVQVNQFALASHFFWGLWALIQAKYSTIDFDFLGYAVVRFNQYFKMKNEVMALKLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y296</accession>
    <name>A0A6I9Y296_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106547895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920636.1">
        <property type="nucleotide sequence ID" value="XM_014065161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y296"/>
    <dbReference type="GeneID" id="106547895"/>
    <dbReference type="KEGG" id="tsr:106547895"/>
    <dbReference type="OrthoDB" id="9908828at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013920636.1"/>
        </source>
    </evidence>
    <sequence length="172" mass="19359" checksum="B99A2E314F16CA3F" modified="2020-10-07" version="1">MKQYVLQIHKLLSTITEPTAEELCEPPTTEEISSTSLVADESDIDKAEHGMKKVCFKVSEEDQEDSGHDTISFRDSYSECNSNRDSVLSYTSVRSNSSYLGSDEMGSGDELPSDMRIPSDKQDKLHGCLEHLFNQVDAINVLLKGPVISKAFEETKHFPIDHTLQVYQQRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4F9</accession>
    <name>A0A6I9Y4F9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LRRC31</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912290.1">
        <property type="nucleotide sequence ID" value="XM_014056815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4F9"/>
    <dbReference type="GeneID" id="106541387"/>
    <dbReference type="KEGG" id="tsr:106541387"/>
    <dbReference type="CTD" id="79782"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042419">
        <property type="entry name" value="LRC31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24109">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24109:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013912290.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="29464" checksum="8A4FF3AE9D13652F" modified="2020-10-07" version="1">MFNSGNSSRRTKQSEAVKMIKKPLPRLIYKSENNFTMDDKATVISLLPDLEEIDLSWNDFIGGALEPLALQFKHLQELKILQLNSCRLTTKDVACLGEALEGISHLEILDLSWNPGIGGSLSLLTSKILKGSKLHTLKVTDCCLTQEDGKSLDAAFQYLSELRILDVSCNKLIGGVFQSLAGHLANLSNLEILNLHQCCITEKDMLVLSQIIPLLSNLRELNLSSNTNAGISTYHLLSRLRFLPKLTSVLLSNCALQHDSFLSLGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y543</accession>
    <name>A0A6I9Y543_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">ORC2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ORC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912815.1">
        <property type="nucleotide sequence ID" value="XM_014057340.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912821.1">
        <property type="nucleotide sequence ID" value="XM_014057346.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912829.1">
        <property type="nucleotide sequence ID" value="XM_014057354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y543"/>
    <dbReference type="GeneID" id="106541799"/>
    <dbReference type="KEGG" id="tsr:106541799"/>
    <dbReference type="CTD" id="4999"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056773">
        <property type="entry name" value="WHD_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24882">
        <property type="entry name" value="WHD_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="8">
        <location>
            <begin position="277"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 2 winged-helix" evidence="9">
        <location>
            <begin position="495"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="79"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013912821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013912829.1"/>
        </source>
    </evidence>
    <sequence length="567" mass="64384" checksum="2AF66B085DBD236E" modified="2020-10-07" version="1">MSNPEIKKINLLEVKFVADEDVLKHISEDAGLKVRKLSVPPTLDIKKWMKNLENSSDDEAQDIFKEEDYVTALDVQESVRNGSSASGGEVFPFQTPKRSNRMAERVSELIQNSAKNAKQNSSKKLEEKTDTPKISSKRSSSKDQEDTKRNDLLSKLSYRTRKRMTALNMCSDSESDYSASNPEEDGDDACSVSADEAVLPKTPIRSQTSSVPPSKETSAKKPKRCSSSNLVEEYFEAHSSSKVLTSDRTLQKLQEKKLDQESLQNLLNNIPVAFAAEREKLMQQYESLFYKWMLQLHLGFNIVLYGLGSKRDLLENFRISLLQDSVHLVVNGFFPNITIKSILNSITEEVLNHMGTFRSPQDQMDFILKSFKEDVSSELYLLIHNLDSKMLRGDKNQQILGQLSSLSNIYLIASIDHINAPLMWDQAKQSMYNWLWYETTTFSPYAEETAYENSLLVQHSGSLALSSLTHVLCSLTSNAKGIFRLLVEYQLENKDNPSYLGLSFQELYQRCREAFLVNSDLTLRAQLTEFRDHKLIRTKRGADGVEYLLIPVDAGILADFLQKDNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5R1</accession>
    <name>A0A6I9Y5R1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor SOX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SOX4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913375.1">
        <property type="nucleotide sequence ID" value="XM_014057900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5R1"/>
    <dbReference type="GeneID" id="106542234"/>
    <dbReference type="KEGG" id="tsr:106542234"/>
    <dbReference type="CTD" id="6659"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048593">
        <property type="term" value="P:camera-type eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22029">
        <property type="entry name" value="HMG-box_SoxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000007">
        <property type="entry name" value="Transcription factor SOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017386">
        <property type="entry name" value="SOX-12/11/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF27">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038098">
        <property type="entry name" value="SOX-12/11/4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="9">
        <location>
            <begin position="59"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="7">
        <location>
            <begin position="59"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="128"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913375.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="44598" checksum="6B31093C6B19E153" modified="2020-10-07" version="1">MVQQTNNAENTEALLAGESSDSGAGIELGIASSPTPGSTASTGGKADDPSWCKTPSGHIKRPMNAFMVWSQIERRKIMEQSPDMHNAEISKRLGKRWKLLKDSDKIPFIREAERLRLKHMADYPDYKYRPRKKVKSGTNSGKPGEKSGGGGSGGGGGNQGAAASAAVSNSSKLAQKKGSAAKLSPSGAGKPHPKLSPSGGGGGGTKAPSSFPSDQPAALLPPEHHSLYKSRAASPGSSSPCSSSSSSSHSSRHLPEKKAKRLYLFSHSSPGGAVPASPTLSTSTEASDPLSLYDEVAAVGGRQDGGEPSASPVGDCSPSDHRSYTSLRAPSPTPSTSHSSSASSSSASCASSSSDDEFEDDLLDLNPSPGFDSMALGSLSSSVLDRDLDFNLEPGSGSHFEFPDYCTPEVSEMISGDWLESSISNLVFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5X9</accession>
    <name>A0A6I9Y5X9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pleiotrophin</fullName>
            <shortName evidence="8">PTN</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PTN</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the pleiotrophin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913545.1">
        <property type="nucleotide sequence ID" value="XM_014058070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5X9"/>
    <dbReference type="GeneID" id="106542366"/>
    <dbReference type="KEGG" id="tsr:106542366"/>
    <dbReference type="CTD" id="5764"/>
    <dbReference type="OrthoDB" id="8818336at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.60.10:FF:000001">
        <property type="entry name" value="Pleiotrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.90.10:FF:000001">
        <property type="entry name" value="Pleiotrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.90.10">
        <property type="entry name" value="Heparin-binding Growth Factor, Midkine, Chain A- C-terminal Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.60.10">
        <property type="entry name" value="Pleiotrophin/Midkine, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000762">
        <property type="entry name" value="Midkine_heparin-bd_GF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020090">
        <property type="entry name" value="PTN/MK_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038130">
        <property type="entry name" value="PTN/MK_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020091">
        <property type="entry name" value="PTN/MK_diS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020089">
        <property type="entry name" value="PTN/MK_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037122">
        <property type="entry name" value="PTN/MK_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020092">
        <property type="entry name" value="PTN_MK_heparin-bd_GF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13850:SF1">
        <property type="entry name" value="PLEIOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13850">
        <property type="entry name" value="PLEIOTROPHIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01091">
        <property type="entry name" value="PTN_MK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05196">
        <property type="entry name" value="PTN_MK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00269">
        <property type="entry name" value="PTNMIDKINE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00193">
        <property type="entry name" value="PTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57288">
        <property type="entry name" value="Midkine"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00619">
        <property type="entry name" value="PTN_MK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00620">
        <property type="entry name" value="PTN_MK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="4 8" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="7 8" id="KW-0497">Mitogen</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026690334" description="Pleiotrophin" evidence="8">
        <location>
            <begin position="31"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine N-terminal" evidence="11">
        <location>
            <begin position="32"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine C-terminal" evidence="10">
        <location>
            <begin position="94"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013913545.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="18703" checksum="2F35F8F15CA4494F" modified="2020-10-07" version="1">MQKQQEQCQIFAVLIFTFIFLLAAVSTTEAGKKEKIEKKVKKSDCGEWQWSVCVPTSGDCGLGTREGTRTGIDCKQTMKTQRCKIPCNWKKQFGAECKYQFQAWGECDLNTALKTRTGNLKRALHNADCQKTVTISKPCGKLIKPKPQEPKKKKKEGKKQEKPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y658</accession>
    <name>A0A6I9Y658_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphotransferase</fullName>
            <ecNumber evidence="12">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GCK</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013920408.1">
        <property type="nucleotide sequence ID" value="XM_014064933.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547695"/>
    <dbReference type="KEGG" id="tsr:106547695"/>
    <dbReference type="CTD" id="2645"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050796">
        <property type="term" value="P:regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000805">
        <property type="entry name" value="Hexokinase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF3">
        <property type="entry name" value="HEXOKINASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="14">
        <location>
            <begin position="195"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013920408.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="35509" checksum="4697DFE029C9908B" modified="2020-10-07" version="1">XVEQILSEFQLREEDLKKVMRRMQKEMDRGLKLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGDDEEGQWKVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLGQHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNIVELLRDSIKRRGILNAVLLAFLAPSLDAGDHKQVYNILTSFELLPSAMDCDIVRMACESVSTRAAQMCSAGLAGVINRMRDSRNEETLKITVGVDGSVYKHHPSFKERFHASVRQLTPGCDIAFLQSEEGSGRGAALISAVAYKMACMIGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6N0</accession>
    <name>A0A6I9Y6N0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger protein 804B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ZNF804B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920833.1">
        <property type="nucleotide sequence ID" value="XM_014065358.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106548053"/>
    <dbReference type="KEGG" id="tsr:106548053"/>
    <dbReference type="CTD" id="219578"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052445">
        <property type="entry name" value="ZnF-G_patch_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17614:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 804B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17614">
        <property type="entry name" value="ZINC FINGER-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="811"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="825"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013920833.1"/>
        </source>
    </evidence>
    <sequence length="1292" mass="146516" checksum="CD3B2BB7EB9945D3" modified="2020-10-07" version="1">MACYLVISSRHLSNGHYRGIKGIFRGPLRKNGSDTADFAEKEKAAARALEDVKANFYCELCDKQYHKHHEFDNHINSYDHAHKQRLKELKQREFARNVASKSWKDEKKQEKALKRLHQLAELRKQSECVAGCRPMFKSPQVVVEQPQQQAQDTFLLAQEDNAKTADEAKSSTSHHSEKLQEPLLNKDQSCIERHRLLKNHMSPAFPCSMNACNRTGVSFSFSKKVHLKLESSASVFSENVEEAHDHIGSPKQKLNQAAKECYTCTHMSSKRKTNVQKRLSTLQDQRDSPELYNLAGKNKPHKENNHNRNRESLETHLPFYKEKQHSLDLECPSSPDIKSRQLVKTRKSIQSFVTSQCQPTSACLQQTDYRHSNILLTECDSEFSGQQSSEGEHSCAIHFNPCIFKCSPDSSKNLASDNETSKNDGLVHEIKPKTFPFLHVLSKDGSKAFHWPIELLLFTKTQPSISYGCNPLYFDFRLPISSKDNGQHEVNPDISIEYSRTMDIDENHVLDLTKKQQLSIRKDNILKPKKNSALYLKKCQPKLYKEKENKINCTAPKYISDDLNENMPNVPAHLDCSQRHCTIATSHHTEMCMRSLAQHFHNCETTTQDEVGENVCIYPLASRAKKETCGKCDLMYSQEQTNLDLVTCTSNINESGKDMLLGCKLDCPDECLEKENNVSFANDFWKFCPLQKPWSDRQSNYSDISTNSESSDTSCYCNHRSNNHSRGNLPFCCKRKHKSIGRQKCKHQKHNCISSSDEADVDCFIHKKSQRPADCTQRNTIKCQRYLRYGHLLQRERAKQSRNRYPVCKHSRSRCTSSQGSCIHDSGNSDTSSSSTQSRGNNSGLLMKESVNIWNKHKKTMRTGEEKKPCSAHSENQNVNYSSKHKHGICSVNWLENEAARQKSLTAKLLLQKVKSKRSQGQIGSTGSFSEACRIDAPCNVPGTSSMESETTLPSQENKHSADMNCVWNHETGEVENNTNQGLTLDNIMGDTTYNNCLLQNLIQRGTTYQTLNMEASTTIKEKTDHLTDETQVLLPSYDPMTGDFPGAFVSHRYSLAPNFTDTKEEYNARADLHQAEEELNYFPDNTMHKFNETEYETDLYNKCISPPLSQHPIIFSPDEIDKYRFLQLQAQQHMQKQLVAKHLKVLPTTEPATFSASPAVQPVPIQQHTSVATLHQTFLQSLALSNGTHPHSSNLTHIHPFSQAHLAPVSIPPFASTFLPTHAALLSGYPFHLVSATPIHPVTQLAIPSLPPTAFIPTWFTPPLNATASSTMHFNPLIHPFFQGQKLPPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7J5</accession>
    <name>A0A6I9Y7J5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Soluble calcium-activated nucleotidase 1</fullName>
            <ecNumber evidence="6">3.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Apyrase homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">CANT1</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ribonucleoside 5'-diphosphate + H2O = a ribonucleoside 5'-phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the apyrase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.6" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013915284.1">
        <property type="nucleotide sequence ID" value="XM_014059809.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915285.1">
        <property type="nucleotide sequence ID" value="XM_014059810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7J5"/>
    <dbReference type="GeneID" id="106543734"/>
    <dbReference type="KEGG" id="tsr:106543734"/>
    <dbReference type="CTD" id="124583"/>
    <dbReference type="OrthoDB" id="25028at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.100:FF:000001">
        <property type="entry name" value="Soluble calcium-activated nucleotidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.100">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009283">
        <property type="entry name" value="Apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036258">
        <property type="entry name" value="Apyrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13023">
        <property type="entry name" value="APYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13023:SF3">
        <property type="entry name" value="SOLUBLE CALCIUM-ACTIVATED NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06079">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101887">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR609283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013915284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013915285.1"/>
        </source>
    </evidence>
    <sequence length="403" mass="45391" checksum="5FC95C19999D2629" modified="2020-10-07" version="1">MPISARPRLQWDDTLNPLRISVGSLPVLASMTKTADPRFRLRWKAIVVTTLALVLVLLLLCFQRSSPANRPVPLNAHNWRLNVLSSERYNDTYPLSPPQRIPEGVRYRIGLIADLDTNSKGPSEHTWFSYLKKGYLTLSASGDHVSVEWDTKDHILESHLAEKGRGMELSELVVFNGKLYAVDDRTGVVYQIDDTRVVPWVILSDGDGTIDKGFKAEWLAVKDEHLYVGGLGKDWTTTTGEVLNQNPEWVKVIGYKGDVAHENWVINYNALRTAAGIKSPGYLIHESASWSERLQRWFFLPRRASHGRYNEQEDEHRGTNLLLSSTPDFTDISVSRIGDVIPTHGFSSFKFVPDTDDQIIVALKSEEDAGHVATYITAFTLDGRCLLPETRIGSVKYEGIEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8C7</accession>
    <name>A0A6I9Y8C7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 184A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM184A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916695.1">
        <property type="nucleotide sequence ID" value="XM_014061220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8C7"/>
    <dbReference type="GeneID" id="106544827"/>
    <dbReference type="KEGG" id="tsr:106544827"/>
    <dbReference type="CTD" id="202915"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="172"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916695.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="23976" checksum="B006504CDC738A4B" modified="2020-10-07" version="1">MRNSASVNALLPSIHSGYLYITIIYNFSVSLALYALFLFYFATMDLLRPFEPVLKFLTIKAVIFLSFWQGMLLAILEKCGVIPEVQIIDGKSVGAGTVAAGYQNFIICIEMLFASIALRHAFTCQVYREKKESATAGLAPLQSISSGLKETMSPQDIVQDAIHNFSPTYQQYTQQSMQETEGGLGQNGHTSPPAEASQSKKTKSLEKRMLIVSDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9X1</accession>
    <name>A0A6I9Y9X1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Liprin-alpha-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PPFIA2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the liprin family. Liprin-alpha subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919415.1">
        <property type="nucleotide sequence ID" value="XM_014063940.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546945"/>
    <dbReference type="KEGG" id="tsr:106546945"/>
    <dbReference type="CTD" id="8499"/>
    <dbReference type="OrthoDB" id="2132119at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09562">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09565">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09568">
        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000003">
        <property type="entry name" value="liprin-alpha-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000002">
        <property type="entry name" value="PTPRF interacting protein alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000004">
        <property type="entry name" value="PTPRF interacting protein alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057892">
        <property type="entry name" value="LIP-1_CC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037620">
        <property type="entry name" value="LIP-1_SAM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037621">
        <property type="entry name" value="LIP-1_SAM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037622">
        <property type="entry name" value="LIP-1_SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF6">
        <property type="entry name" value="LIPRIN-ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25526">
        <property type="entry name" value="LIP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="929"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="1052"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="1133"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="739"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="791"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="273"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="530"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="682"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="739"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="791"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013919415.1"/>
        </source>
    </evidence>
    <sequence length="1282" mass="146159" checksum="D0D4F01B5991BD06" modified="2020-10-07" version="1">MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLSLAQQRLQDVIYDRDSLQRQLNSALPQMTVAAGTSIENNIQRTISCPNEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSTLEEELTAANQEIIALREQNAHIQRKITSGEGPPDTEHIKGMEPGQKIHEKRLSNGSIDSNDETAQLVELQELLEKQNYEMAQMKDRLAALSSRVGEVEQDAETARKDLIKTEELNSKYQRDIREAMAQKEDMEERITTLEKRYLSAQRESTSIHDMNDKLENELANKEAILRQMEEKNRQLQERLELAEQKLQQTMRKAETLPEVEAELAQRIAALTKSDPTSSTSSDDYQYVMEAKLQEMISIRRKAEERHGNIEERMRHLEGQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEEKNVLIQESENFRKNLEESLHDKERLAEEIDKLRVELDQLKIRAGSLIEPTLSRTHLDSSAELRYSVGSLVDSQPDYRSTKVIRRPRRGRMGIRRDEPKVKSLGDHEWNRSQPVGVLSSHHFESDTEMSDIDDDDRDTLFSSMDLLSPSGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVGSVSLEGLNLARVHPGTSITGSITASSLASSSPPSGHSTPKLTPRSPAREMDRMGIMTLPSDLRKHRRKIAVEEDGHEDKATIKCETSPPPTPRAIRMTHTLPSSYHNDARSSLIASLEPESIGFGSVNSSQDSLHKAPKKKGIKSSIGRLFGKKEKARLGQLRGYIETEAAAQESLGLSKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLAAPTKTKDNEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKKLNYDRQELERRREASQHEIKDVLVWSNDRVIRWIQAIGLRDYANNVLESGVHGSLIALDDNFDYSSLALLLQIPTQNTQARQILEREYNNLLALGTGRRLDESDDKNFRQGSSWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGQSRKMATDVASSRLQRLDSSTVRTYSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9Y0</accession>
    <name>A0A6I9Y9Y0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Double-stranded RNA-specific adenosine deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ADAR</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919460.1">
        <property type="nucleotide sequence ID" value="XM_014063985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9Y0"/>
    <dbReference type="GeneID" id="106546970"/>
    <dbReference type="KEGG" id="tsr:106546970"/>
    <dbReference type="CTD" id="103"/>
    <dbReference type="OrthoDB" id="10268011at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19913">
        <property type="entry name" value="DSRM_DRADA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19915">
        <property type="entry name" value="DSRM_DRADA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000005">
        <property type="entry name" value="Putative double-stranded RNA-specific adenosine deaminase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044456">
        <property type="entry name" value="ADAR1_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044457">
        <property type="entry name" value="ADAR1_DSRM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042371">
        <property type="entry name" value="Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF107">
        <property type="entry name" value="DOUBLE-STRANDED RNA-SPECIFIC ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02295">
        <property type="entry name" value="z-alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00550">
        <property type="entry name" value="Zalpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50139">
        <property type="entry name" value="Z_BINDING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="Z-binding" evidence="9">
        <location>
            <begin position="169"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Z-binding" evidence="9">
        <location>
            <begin position="281"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="500"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="617"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="733"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="A to I editase" evidence="10">
        <location>
            <begin position="871"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="355"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="587"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="683"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="589"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919460.1"/>
        </source>
    </evidence>
    <sequence length="1217" mass="133790" checksum="51910523E33134A5" modified="2020-10-07" version="1">MYISVPPSLLQGQEVSLPALGRGDAMYRSHPGQKRSGSSTSAENAFPPALLPHPRAMSRFPAGDTASDQTVARSGGHEDLHQRQVLFLRGELSEAPLWKPSAQRSPAPRTFFPPPRSNLWFNYREPVRRPPGKGGWQAPKPPSPPKPSYFAACPKGGVDQLVSNFQGLTLGQQDLEGDLLEILRDLKSGQTISARQLAQRLRAQKKVVNRHLYKLYRRGQLSRSGTTPPLWRLADGRDSGEASREGRPASREKNPSPFFGLGTEEEGGGSSSSDQEDSFDSPDMAEIKEKICSFLLGVTDSTALNLAKNIGLTKARDVNATLLALEKLGDVHKENSSPPKWSLTDNKRKRMQVKMKAEEVRQAVTVDNEPPAPCTEPEAPAEVPSPRPSPPPENEEKKIENGQQAAEVTEPSGGGGGGCPPPWSSPSSKRPRYRRFSPYGYFEASSWATDDIPEDMNAISTQDESRYIMESPLYPSYSAQLDTAFQCSPLEKLMDCQKKNPVSGLIEYTQYMYQHCEFVLLKQSGPSHEPRFKFQAKIDTRHFPPAEAGSKKLAKQEAAAIAMKILLHEAQHEGEDIMGPETSLYEDTSLGEEETPDQADPEASSTPPQPNLVCGKNPISALMEYAQKLGSVCEFKMLSQEGPAHDPKFRYCVKVGERIFPAVVSNSKKGAKQMAAEAAMRGLSGETTSSPAQVEAPSEPTLDVQGSQLANPEESRTVSAKGVGELIKYLNTNPVSGLLEYARANGFAAEFKLINQTGPPHDPKFVFQAKVGGRWFLPVSAHSKKQGKQEAADAALRVLIGEKEKSEHAAEGLGITELPVSGSTLHDQIAMLSHQRFNALTARIQHSLLGRKILAAIIMRRGQEGLGVVVSIGTGNRCVKGEELSLKGETVNDCHAEIISRRGFVRFLYNELMNFDPLAPEESIFQLAEDRKLKIKDDVSFHLYISTAPCGDGALFDKSCSEQANTAGEDQHQPLFETPRQGKLRTKVENGEGTIPVESSDIVPTWDGIQHGERLRTMSCSDKILRWNVLGLQGALLSHFIHPVYLRSVTLGYLYSQGHLTRAICCRMSRDGSTFASRLPAPYLVNHPEVGRVSVYDSARQTGKTKESSINWCLPDGTNVEVLDGTKGKVDGPKLDVSRVSKQSLFQLFRQLCVKMVREDLKKFAAYSEAKESAADYQSAKQQFFWGLQEMGYGSWICKPQEEKTFLLPEPSTPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAP9</accession>
    <name>A0A6I9YAP9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein phosphatase 1 regulatory subunit 32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PPP1R32</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920805.1">
        <property type="nucleotide sequence ID" value="XM_014065330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAP9"/>
    <dbReference type="GeneID" id="106548024"/>
    <dbReference type="KEGG" id="tsr:106548024"/>
    <dbReference type="CTD" id="220004"/>
    <dbReference type="OrthoDB" id="9980630at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031410">
        <property type="entry name" value="SAXO4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34349">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34349:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15691">
        <property type="entry name" value="PPP1R32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920805.1"/>
        </source>
    </evidence>
    <sequence length="425" mass="46584" checksum="6EE7EAAC3862FB19" modified="2020-10-07" version="1">MGRLPLGAVSPYVKTSSGGSMDPMKFYCTSYGAAYGQEGFRPRNGFHFGAGYKSNYRPVVQYNPNLDKIDNPAVGKLLQDNYESTTNTHFQPLQLPDGKYPLPWSVHQPGSGYVREKPLTFPTTKTVKKVHFDTANQGAEAMIGLEPRHVPALHNTYGKGSVEAENARHGPQFMSTEYGSKYRFNIPGQPDFLQNKTIGAKEESGFTEGCLANPIVEKLASQNSNPGISIMKADYLPSSFPHGDEFLPVLSKGSERGSGFSRQKDLRLGSSGPPPNAEASGPLSHSQFQGLFRQPQTQTNLLGREYVGNKESSGFSTNNLNYVTPAYDPNLPNRFLTSYNSKFYETISKGVDREGWTRGGIQPQQAGGFATNNHGTGLGSDPNATETLRTVHPHVGRTITTVDPFYRDSPHDRRFAALHQTAVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAR2</accession>
    <name>A0A6I9YAR2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">A disintegrin and metalloproteinase with thrombospondin motifs 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ADAMTS15</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920870.1">
        <property type="nucleotide sequence ID" value="XM_014065395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAR2"/>
    <dbReference type="GeneID" id="106548080"/>
    <dbReference type="KEGG" id="tsr:106548080"/>
    <dbReference type="CTD" id="170689"/>
    <dbReference type="OrthoDB" id="412680at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF39">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 19" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="17">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="13 16">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="29"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="218"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="222"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013920870.1"/>
        </source>
    </evidence>
    <sequence length="643" mass="70783" checksum="8803EDB7F1EBFD30" modified="2020-10-07" version="1">MILPLYNALDLCGATTCDTLGMADVGTMCDPKRSCSVIEDDGLPSAFTTAHELGHVFNMPHDNVKACEEVFGKLKTNHMMSPTLIQIDRINPWSPCSTAIITDFLDSGHGDCLLDQPTKPISLPQELPGSSYNLNQQCELAFGIGSKPCPYMQYCTKLWCTGKARGHIMCQTRHFPWADGTSCGEGRFCIKGACVERHNISKYRVDGNWGKWAPYGSCSRTCGGGVQLAKRQCNNPPPANEGKYCEGVRVKYRSCSLDPCFASVPGKSFREEQCEAFNGYNHSTNRLTAIVSWVPKYSGVSPRDKCKLICRANGTGYFYVLAPKVVDGTPCSPDSSSLCVQGKCIKAGCDGKLGSKKKFDKCGVCGGDNKSCKKVSGLFTKPMHGYNFVVLLPAGASSIDIRQRGYKGLISDDNYLALKNAHGKYLLNGHFIVSAVERDLIVKGSVIRYSGTSTAVESLQAFMPIQEPLTVEVLSVGKMTPPRVRYSFYLPKESKEDKSSYHRKDGKRPPVLNNSILSLSNRLDLGQLDYKRPAYKWATTNWEDCSVTCGNGLQKRMVLCRDALGQLASSCDATQRPVDIRICGDPCPTWEASPWSSCSKTCGRGFKRRILRCTGRGGTLLPRERCNLRKKPQELDFCTLRPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAY1</accession>
    <name>A0A6I9YAY1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Myosin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106548378</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921215.1">
        <property type="nucleotide sequence ID" value="XM_014065740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAY1"/>
    <dbReference type="GeneID" id="106548378"/>
    <dbReference type="KEGG" id="tsr:106548378"/>
    <dbReference type="OrthoDB" id="9036525at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="1"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921215.1"/>
        </source>
    </evidence>
    <sequence length="481" mass="54902" checksum="FCAFE6D25999F165" modified="2020-10-07" version="1">LKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKRPELIEMLLITTNPYDFHFVSQGEITVASIDDQEELMATDSAIDILGFTAEEKTAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLMNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNNVGALAKAVFEKMFLWMVVRINQQLDTRQSRQHFIGVLDIAGFEIFDYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDVSFKNKLYDQHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNETVVGLYQKSSMKTLALLFADRPVEEGKKAAKKKGSSFQTVSALFRNVLEFSVYDSDAVTEDDHLFTINFDVARLPDNEKVLMNFKCDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBD1</accession>
    <name>A0A6I9YBD1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-serine deaminase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-threonine dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106548560</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_013921427.1">
        <property type="nucleotide sequence ID" value="XM_014065952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBD1"/>
    <dbReference type="GeneID" id="106548560"/>
    <dbReference type="KEGG" id="tsr:106548560"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000044">
        <property type="entry name" value="Phenylserine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF19">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="6">
        <location>
            <begin position="2"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921427.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="38646" checksum="2B1388D7D81DE924" modified="2020-10-07" version="1">MEIYLKKELLQYTGTAKERGVLYLLMSLREDQQRNGVIVASDCNFSMAVAYHATELHIPVFVIMSTCTPPTRVKMCREYGAMVISYGSTIRDSHLHARRLAQENNYLYLEEDDSAIYLSGLGTMGLEIYEQVPKLDAVILPAGNHSGLLASNSAAFKHLNQHVFVVGVELENIHVDQQSLKMGQPVDDHTYSHSQFYRVHEAQQHFWRYLPILPALSKDSLKVELSGFGTNSFQLTGNWVDKVVTVREEDILIAMLRLLEYERVAVDAEGAIALAALVSGKLPELKGKRVAVAVSNGNMELPLMRQCMDQALTLDNRVCKFTVTTVDCPGEISKLLETLAREEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBF3</accession>
    <name>A0A6I9YBF3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mesothelin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MSLN</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mesothelin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921461.1">
        <property type="nucleotide sequence ID" value="XM_014065986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBF3"/>
    <dbReference type="GeneID" id="106548590"/>
    <dbReference type="KEGG" id="tsr:106548590"/>
    <dbReference type="CTD" id="10232"/>
    <dbReference type="OrthoDB" id="9329195at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010335">
        <property type="entry name" value="Mesothelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026664">
        <property type="entry name" value="Stereocilin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412:SF6">
        <property type="entry name" value="MESOTHELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412">
        <property type="entry name" value="STEREOCILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06060">
        <property type="entry name" value="Mesothelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921461.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="46462" checksum="0C2785356FFD65B3" modified="2020-10-07" version="1">MSGDSIAATGEKFLKQLSQCQHLTPDQIKAIQQIFSNGNTSFGPPSTWTPSVLDQLSQLILILGPSILQSIPKNVLMPWLKNSNLHSDLSKNQVGRIRESLKSSRTKRETECPKDKAIPEEILNEDQIDELLFYSPMDLKLCLTKEILVNNLNVFSENAFTYEQLQAVKDKLDEIFPKGYPPAVISNLGLIENLIDEKNIKEWTINSTITVRALLNSVSSDELRIAVIQRYTESGAQIDSDFLDVLGPYICLLNEGQLSMITERMIKMATLLDPSNCSSAIKDHLYPKAKRAFSDRHYEYSEYYNRIKPFLGGAPGEDLRALSKNDVNMDIQTFLGLKSSSLMELTPENVKGLLGRNLNNLQDKQNIPLIREWIQTRKQSDLNSLGLGLQGGLPEGFVVLKSKTTRASTFRLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBV3</accession>
    <name>A0A6I9YBV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rho GTPase-activating protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Rho-type GTPase-activating protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ARHGAP10</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">GTPase-activating protein that catalyzes the conversion of active GTP-bound Rho GTPases to their inactive GDP-bound form, thus suppressing various Rho GTPase-mediated cellular processes. Also converts Cdc42 to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death. Associates with MICAL1 on the endosomal membrane to promote Rab8-Rab10-dependent tubule extension. After dissociation with MICAL1, recruits WDR44 which connects the endoplasmic reticulum (ER) with the endosomal tubule, thereby participating in the export of a subset of neosynthesized proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921544.1">
        <property type="nucleotide sequence ID" value="XM_014066069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBV3"/>
    <dbReference type="GeneID" id="106548649"/>
    <dbReference type="KEGG" id="tsr:106548649"/>
    <dbReference type="CTD" id="79658"/>
    <dbReference type="OrthoDB" id="3183924at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01249">
        <property type="entry name" value="BAR-PH_GRAF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000157">
        <property type="entry name" value="Putative rho GTPase-activating protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000006">
        <property type="entry name" value="Rho GTPase activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000001">
        <property type="entry name" value="Rho GTPase-activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000055">
        <property type="entry name" value="rho GTPase-activating protein 26 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047225">
        <property type="entry name" value="PH_GRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF5">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="265"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="17">
        <location>
            <begin position="388"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="709"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="626"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="641"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013921544.1"/>
        </source>
    </evidence>
    <sequence length="767" mass="88271" checksum="8ADE2902136EC748" modified="2020-10-07" version="1">MGLQPLEFSDCYLDSPWFRERIRAHEAELEKTNKFIKELLKDGKTLIAATKNLSAAQRKFARSLRDFRFECIGEAETDDERYIDASLHEFSNFLKNLEEQREIMALSVTETLIKPLEKFRKEQLGAVKEEKKRFDKETEKNYSSLEKHLNMSAKKKEVQILEADYQIEQNRKHFYELLLVYVCKLQEIQERKKFEFVEPVLSFFQGMFTFYHQGYELAKDFNHYKMDLQINIQNTRTRFEGTRSEVEDLMNKIKQNPKKHKRANQFAMEGYLYVQEKRPAPFGSSWIKHYCMYKKESKKFTMLPFEHRSGGKSGELEVYLLQNCTKRNTDSIDRRFCFDMEVIERPGTPFTLQAFSEEDRNMWFEALDGKETVFLNLNKANTQKEGSVQLDKIGFTILRKCISAIEKRGINDQGLYRVVGVSSKVQRLLNLLMDAKTCDEVDLESNTEWEVKTITSTVKQYLRSLPEPLMTYELHGEFITPAKSGSPESRFKVIHSLVHKLPEKNKEMLDIVVKHLANVSDHAKKNLMTVANLGVVFGPTLMRPQEETVAGLMDLKFQNIVVEILIENHEKIFHMLPDACFPEPTLVPISPPNAPPRQSRRQGQRNKRPVAVYNLCLELDDSDNLSPAKEYTHSGSMDSLSSQSPTTAAPTSPQDTEKNNLITDSGNSSSEVHSSMVSWINLKSTTAQSPVTTNLSFLFPSSATETDRPVGRKARAVYPCEAEHSSELSFQTGAVFEDVHLSREPGWLEGTLNGKRGLIPQNYVQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCF1</accession>
    <name>A0A6I9YCF1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhombotin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">LIM domain only protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LMO2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921818.1">
        <property type="nucleotide sequence ID" value="XM_014066343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCF1"/>
    <dbReference type="GeneID" id="106548856"/>
    <dbReference type="KEGG" id="tsr:106548856"/>
    <dbReference type="CTD" id="4005"/>
    <dbReference type="OrthoDB" id="6352355at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09384">
        <property type="entry name" value="LIM1_LMO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09385">
        <property type="entry name" value="LIM2_LMO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000059">
        <property type="entry name" value="LIM domain only 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000016">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF3">
        <property type="entry name" value="RHOMBOTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="29"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="93"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013921818.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="18491" checksum="0CDFC9D6BC86A59F" modified="2020-10-07" version="1">MSSSAIERKSLEPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCEKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGH6</accession>
    <name>A0A6I9YGH6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trafficking kinesin-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106550022</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the milton family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923276.1">
        <property type="nucleotide sequence ID" value="XM_014067801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGH6"/>
    <dbReference type="GeneID" id="106550022"/>
    <dbReference type="KEGG" id="tsr:106550022"/>
    <dbReference type="OrthoDB" id="10067624at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050811">
        <property type="term" value="F:GABA receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098957">
        <property type="term" value="P:anterograde axonal transport of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048311">
        <property type="term" value="P:mitochondrion distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006933">
        <property type="entry name" value="HAP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051946">
        <property type="entry name" value="Intracell_Traff-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022154">
        <property type="entry name" value="TRAK1/2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751:SF13">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04849">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12448">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01424">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01423">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAP1 N-terminal" evidence="7">
        <location>
            <begin position="5"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking kinesin-binding protein C-terminal" evidence="6">
        <location>
            <begin position="206"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="41"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013923276.1"/>
        </source>
    </evidence>
    <sequence length="701" mass="77221" checksum="F0FDD83F27A663CF" modified="2020-10-07" version="1">MGKLIKINEILKFLKACHLKTETMTYEDKEHQLVTDCVKEITCISAEIEEANAQIARMSEELMRKDEELINYQEEISSLLSQIVDLQHKIKEHVIEKEELKLHLQASKDAQKQLTAELHEVQERNVECLGMLQESQEEVKALRSKTSQAAVLCRPQLCGAFPVESLAAEIEGTMRKVISLDEESLGKQKVQQKRIFDTVKLANDMRGRSASCPVSLSIPGSNRSSVVMTAKPFHSGLQYMEGKGVQALKASTEGVSKETQKLSHPGIPAEDDVATALHKLNLRCQNYLSEKWFFEEELKREIRFLADQKEELVVCSTPVNNSLSLNSSSERFDFSGSSSSMWSLLPEKLQIVKPLEGSQTLFHWQQLAQPNLGTILDSRPGVVTKDFAPLSQEDVYYLSDLEEDEERDEGITFQVSSQEESKQAVPKSVPGIFLPPVKLAAVPHTASNPGKCLSSTNSTFTFTTCRILHPSDITQVTPSTLYSPLCSGSCSSTGSVITSSPPMLYKLSTGEFLTSRRDSTTTFSSTRSLAKVLQEQGISATVYNTPVQLETPLLPHPKMPAVPSTPPNSPLHSPCSSPQPFESRACVSENFLASRPAETLLHELYGQNLSNPDAGQLKMNLVERLKKLGIARAVKQPEAGGYRKSQRENSCLQRQGSGVYVSAGSNLMGGLRRNHSLPVLIGALAGPVSASSTTMEVLKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH80</accession>
    <name>A0A6I9YH80_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ERI1 exoribonuclease 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ERI3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923235.1">
        <property type="nucleotide sequence ID" value="XM_014067760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH80"/>
    <dbReference type="GeneID" id="106549993"/>
    <dbReference type="KEGG" id="tsr:106549993"/>
    <dbReference type="CTD" id="79033"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="4">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013923235.1"/>
        </source>
    </evidence>
    <sequence length="160" mass="18157" checksum="6D57B09C1B78F4FD" modified="2020-10-07" version="1">MEIESTFHMYVQPVVHPQLTPFCTELTGIIQAMVDGQPSLQQVLERVDEWMAKEGLLDPTVKSIFVTCGDWDLKVMLPGQCQYLGLPVADYFKQWINLKKAYSFAMGSWPKNGLLDMNKGLNLQHIGRPHSGIDDCKNIANIMKTLAHRGFIFKQTSKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHB7</accession>
    <name>A0A6I9YHB7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Collagen alpha-1(IV) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">COL4A1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923723.1">
        <property type="nucleotide sequence ID" value="XM_014068248.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550350"/>
    <dbReference type="KEGG" id="tsr:106550350"/>
    <dbReference type="CTD" id="1282"/>
    <dbReference type="OrthoDB" id="10071882at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.240.10:FF:000001">
        <property type="entry name" value="Collagen IV alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.240.10">
        <property type="entry name" value="Collagen IV, non-collagenous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001442">
        <property type="entry name" value="Collagen_IV_NC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036954">
        <property type="entry name" value="Collagen_IV_NC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF6">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01413">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00111">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51403">
        <property type="entry name" value="NC1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="8 14" id="KW-0176">Collagen</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026829499" evidence="11">
        <location>
            <begin position="29"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="domain" description="Collagen IV NC1" evidence="12">
        <location>
            <begin position="1430"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="51"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="259"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="467"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="679"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="789"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="822"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="871"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="921"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1071"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1154"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1175"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1225"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1255"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1303"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1337"/>
            <end position="1346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923723.1"/>
        </source>
    </evidence>
    <sequence length="1654" mass="159900" checksum="C9A6F8E611B85DE1" modified="2020-10-07" version="1">MGSSLAGPGIALLLAVLLVPGRRSGSEAAGGCSGCGKCDCNGVKGQKGERGLPGLQGLVGFPGVQGPEGPPGASGTKGDTGEPGLPGTKGTRGPPGSAGYPGNPGLPGIPGGDGPAGPPGIPGCNGTKGERGSHGPPGLPGLPGNLGLPGVPGIKGDPGEIISILPGGFQKGEKGLPGHTGQPGTPGLPGNTGPIGPPGPPGRHGIPGPPGPPGEKGHMGLSFQGPQGEKGERGVMGPPGPQGTDTQHQEKGTIVIQGDKGDPGEKGDDCIPGRPGKDGEDGEKGERGLPGHPGITGHPGVLGSKGDKGIVGPPGPPGIVTDERDEEVFGEKGDSGPQGFPGRKGEPGPPGFQGSPGPPGPPGQAIPGPAGFPGERGRKGDQGPPGTSLPGPKGRDGFTGPRGSPGPPGLPGGTDGIGCERGLPGDPGLQGTPGQSGYTGERGEKGEKGESCSECDGAGFPGPTGPQGPQGEQGIPGLPGAKGDRGSPGFEGIPGVAGPPGVPGLIGIPGPKGEPGEIFYDSIVKGEKGEPGLPGHPGTPGKEGRPGKDGVPGSPGPKGASGTIGLKGDRGLPGISGLPGSPGDRGFPGPPGVGNPGPQGDKGDKGVPGIPGTSGLPGPKGEAGRVISLPGPPGARGFPGPAGNPGFQGDKGDPGIPGRPGLPGEKGLAGPPGIGFPGPSGPPGFPGQPGPQGSRGEKGEHGSQGVPGASGAKGQKGEAGLGLPGPKGFPGPRGRDGIPGSKGDQGYPGVSGEKGFPGIPGQPGSQGELGPPGLPGVQGSPGEPQMGSPGPPGPSGQSGPPGPIGPPGIKGERGHPGIPGLDTPGPKGDKGTPGTQGLSGVPGLPGLPGLQGQQGHPGVPGVKGEMGIMGTPGIQGLPGPIGTPGTPGEKGSLGFPGVAGPRGDPGLKGEKGEVGLPGEPGKMEKVDMENMKGQKGEQGDKGTKGLIGEKGTPGISGRPGMPGKDGDPGLPGQAGPKGDSGSPGFQGEAGFPGEKGSMGEMGLPGLPGEKGFQGLPGEAGQSGFPGVKGEKGRKGDSGLPGIGFPGAPGEKGEQGWPGNSGLPGDKGEKGIPGISGIPGSPGIKGQSGLPGSPGVPGLAGEKGQKGTPGFSGVPGLKGQPGDPGVQGPHGTAGEKGEPGFDGIPGTPGSKGDSGLPGRGFPGIPGGKGERGPKGDVGSPGSPGSSGVPGTKGDIGFVGPQGPPGQPGIPGLPGNIGEGPKGDRGPQGQPGLQGLPGPQGFPGVSGLPGEKGEKGSQGWPGQSGQPGPKGDLGVQGIPGSPGFPGTTGQKGDMGPPGIPGPQGLKGSSGFLGSKGVQGDQGFPGNKGLPGPPGPPGPFQLLKGEPGLPGTPGPAGPKGIPGFPGPKGQQGVTGPSGLTGPPGIQGIDGTPGQKGDKGPVGPTGPRGFPGPPGPEGMPGVMGPPGRPSMAHGFLVTRHSQTTEDPSCPFGTRVMFYGYSLLYVQGNQRAHGQDLGSAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPLTGDSIRPFISRCAVCEAPSMVIAVHSQTVQIPPCPEGWNSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRSAPFVECHGKGTCNYYANAYSFWLATIERNEMFKKPTSSTLKAGELHSNISRCQVCMKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIA7</accession>
    <name>A0A6I9YIA7_9SAUR</name>
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        <submittedName>
            <fullName evidence="4">Voltage-dependent calcium channel subunit alpha-2/delta-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550534</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923942.1">
        <property type="nucleotide sequence ID" value="XM_014068467.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106550534"/>
    <dbReference type="KEGG" id="tsr:106550534"/>
    <dbReference type="OrthoDB" id="9034035at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
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    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF59">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Voltage-dependent calcium channel alpha-2/delta subunit conserved region" evidence="2">
        <location>
            <begin position="12"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013923942.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15000" checksum="54EB6C39AD538819" modified="2020-10-07" version="1">HKHKKHDALQPCDTEYPVFVYEPAIKEANGLIECGACQKMFLAQQIMNSNLLLLVTDATCDCSIFPSFALKAKEVKYNNSVKCDRMRSQKLRRRPDSCHAFHPEENAQDCGGAAAISASSTLLLFLLATTALLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIT9</accession>
    <name>A0A6I9YIT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15 16">G-protein coupled receptor 35-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">LOC106550685</name>
    </gene>
    <organism evidence="13 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924109.1">
        <property type="nucleotide sequence ID" value="XM_014068634.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924118.1">
        <property type="nucleotide sequence ID" value="XM_014068643.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924126.1">
        <property type="nucleotide sequence ID" value="XM_014068651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIT9"/>
    <dbReference type="GeneID" id="106550685"/>
    <dbReference type="KEGG" id="tsr:106550685"/>
    <dbReference type="OrthoDB" id="6086428at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000142">
        <property type="entry name" value="G protein-coupled receptor 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF97">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="30"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013924118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013924126.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="35344" checksum="FA1C1F8594FFA56C" modified="2020-10-07" version="1">MSLRNDTSERSIHIAEAIIYVPVFFIGVILNVFALRIFCCKLVKWTETRVYMTNLAITDCLFLFTLPFRLVSNFLYKSIYNIYLNKLCMVLETAYFISRYMSIFLITIIALDRYIAIRYPLQAKTIRSPLKSALVCGLFWIIIITIALASIYVNNEKGKICFQTIQRPPSETSFAVVIWGFFIPLIILSFCSVQIIKKLTRKKKTHLNEKKVIQKAINIILANITTFIICFLPLHVVYFVKYINFKNATDANLNHDLSLKVAIILANTNCCLDAFCYYFVSQEFKEASVVRKTQNQAPENQLTEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJV4</accession>
    <name>A0A6I9YJV4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GABBR2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924653.1">
        <property type="nucleotide sequence ID" value="XM_014069178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJV4"/>
    <dbReference type="GeneID" id="106551151"/>
    <dbReference type="KEGG" id="tsr:106551151"/>
    <dbReference type="CTD" id="9568"/>
    <dbReference type="OrthoDB" id="2150267at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041689">
        <property type="entry name" value="GBR2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002457">
        <property type="entry name" value="GPCR_3_GABA_rcpt_B2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF74">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18455">
        <property type="entry name" value="GBR2_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01178">
        <property type="entry name" value="GABAB2RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="338"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="231"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="400"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013924653.1"/>
        </source>
    </evidence>
    <sequence length="583" mass="66167" checksum="DD96C7749AD962C2" modified="2020-10-07" version="1">TPQQYEEEYDQRRGNVEPSKFHGFAYDGIWVIAKTLQQAMKILNATNRNQKIEDFNYTNKELGKIFLDAMNQTSFFGVTGQVMFRNGERMGTIKFTQFQDKKEVKVGEYNAILDSLEIINNSIKFHGKEPPKDRTIIQKQLRKISLPLYSILSALTILGMIMASAFLFFNIKNRNQKLIKMSSPYMNNLIILGGMLSYASIFLFGLDGSFVSEKTFETLCTVSNSEPFYIKFIIKDQKLMVIVGGMLLIDLCILICWQVVDPLRRTVEKYNMEPDPAGRDISILPILEHCENTHMTIWLGIVYAYKGLLMLFGCFLAWETRNVSIPALNDSKYIGMSVYNVGIMCIIGAAVSFLTRDQPNVQFCIVALVIIFCSTITLCLVFVPKLITLRTNPDAATRNRGLQFTQNQKKDDSKTSTSVTSVNQASTSRLEALQSENHQLRMKITELDKSLEEVTMQLQDTPEKTTYIKQNHYQELNDILSIRNFTESKDGEKTVLKNHLDQNPQAQWNTTESSRASKDFIEDINSPEHIQRRLSLQLPILHHAYLPSIGGVDASCASPCVSPSASPRHRHVPPSFRVMVSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK61</accession>
    <name>A0A6I9YK61_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein unc-80 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924419.1">
        <property type="nucleotide sequence ID" value="XM_014068944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YK61"/>
    <dbReference type="GeneID" id="106550955"/>
    <dbReference type="KEGG" id="tsr:106550955"/>
    <dbReference type="OrthoDB" id="9428549at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924419.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="21847" checksum="D92E179720CD1009" modified="2020-10-07" version="1">MDLDILFDGEYDDFMQKINLCYGREQALDLSSKPIHHSTTEAGVDQKRSVTFTEAQSEASSSKSDPTPITAQCSKKNIPAEDSKHATNKSAVKSSPTIIVADLHSPISSHQIETLATGKDTKKEEDSEPKPALAHSTSPTTQLSGAEDFTGLETTSLLQHEDTVLHISEDNGMENPLLSSQFNFTQVEMGQTDVGLDESHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKT6</accession>
    <name>A0A6I9YKT6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">S phase cyclin A-associated protein in the endoplasmic reticulum-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106551292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924847.1">
        <property type="nucleotide sequence ID" value="XM_014069372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKT6"/>
    <dbReference type="GeneID" id="106551292"/>
    <dbReference type="KEGG" id="tsr:106551292"/>
    <dbReference type="OrthoDB" id="9048734at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000680">
        <property type="entry name" value="S phase cyclin A-associated protein in the endoplasmic reticulum"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31434">
        <property type="entry name" value="S PHASE CYCLIN A-ASSOCIATED PROTEIN IN THE ENDOPLASMIC RETICULUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31434:SF2">
        <property type="entry name" value="S PHASE CYCLIN A-ASSOCIATED PROTEIN IN THE ENDOPLASMIC RETICULUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="2">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924847.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="21409" checksum="E04750119738C35C" modified="2020-10-07" version="1">ERKRALEAERQARVEELLMKRKGQEARIEQQRQEKEKAREDAARERARDREERLAALTAAQQEAMEELQKKIQLKHDESIRRHMEQIEQRKEKAAELSSGRHANTDYAPKLTPYERKKQCSLCNVVISSEVYLFSHIKGKKHQQAVRENSSIQGRELSDEEVVIAVVNGSFDTTHEHLSIPEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKW5</accession>
    <name>A0A6I9YKW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enhancer of polycomb homolog 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106551434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925013.1">
        <property type="nucleotide sequence ID" value="XM_014069538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKW5"/>
    <dbReference type="GeneID" id="106551434"/>
    <dbReference type="KEGG" id="tsr:106551434"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013925013.1"/>
        </source>
    </evidence>
    <sequence length="152" mass="17647" checksum="F43CB823DF96DEA2" modified="2020-10-07" version="1">MLKLRRDLSHAVTILEIMKKREKSKRELLHLTLEIMEKRYNLDDYNGEIMSEVMVHQQPIKPTYAIPVISVPNSTQFKHPESMELKEFKINKQEKADAIRPKRKYEKKPKIVSSSAAIATQQSIPAALPIFNVKDMNQYDFPSSDEEPLSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP80</accession>
    <name>A0A6I9YP80_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent oxidoreductase domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FOXRED2</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925714.1">
        <property type="nucleotide sequence ID" value="XM_014070239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP80"/>
    <dbReference type="GeneID" id="106552053"/>
    <dbReference type="KEGG" id="tsr:106552053"/>
    <dbReference type="CTD" id="80020"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013925714.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="22125" checksum="FBCCB161DF848FBD" modified="2020-10-07" version="1">MEYGKNFSGPDKDVFYYNRAVGEPEHAWQSNFLHPVIYYYKQLPTEREMRLCPPDWPLPRPDAIHHIVEDFLTDWTAPNAHVLPLRRFLENCLETDLRRFYAESCFLFAFTHLRLPPFCQQEYMKKQGLVGSEKLQHHRVAAELIEHASIKSQDEQESGTMESHNQLLKSTKISSHQLQHLTSTKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ01</accession>
    <name>A0A6I9YQ01_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional enhancer factor TEF-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106552101</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925760.1">
        <property type="nucleotide sequence ID" value="XM_014070285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ01"/>
    <dbReference type="GeneID" id="106552101"/>
    <dbReference type="KEGG" id="tsr:106552101"/>
    <dbReference type="OrthoDB" id="10006572at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000001">
        <property type="entry name" value="Transcriptional enhancer factor TEF-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YAP binding" evidence="6">
        <location>
            <begin position="56"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013925760.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="30423" checksum="638F92C3E435E541" modified="2020-10-07" version="1">MLQTGQPGSSQDIKPFVQQAYPIQPTVTTPISGFEPTSAPAPSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDTGAFYGVTSQYESSENMTITCSTKVCSFGKQVVEKVETEYARFENGRFVFRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQC8</accession>
    <name>A0A6I9YQC8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transcription factor Sp1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SP1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926723.1">
        <property type="nucleotide sequence ID" value="XM_014071248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQC8"/>
    <dbReference type="GeneID" id="106552859"/>
    <dbReference type="KEGG" id="tsr:106552859"/>
    <dbReference type="CTD" id="6667"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22539">
        <property type="entry name" value="SP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000061">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF16">
        <property type="entry name" value="TRANSCRIPTION FACTOR SP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="589"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="619"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="649"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="108"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="532"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926723.1"/>
        </source>
    </evidence>
    <sequence length="750" mass="79108" checksum="87FF0998E6ABB70A" modified="2020-10-07" version="1">MNFRGLKSGWFLHHWHEEVAKAVRDHDISEDMVVIKTEKVDEGCSNDGGNCEAHESQPSPLALLAATCSRIESPNDNSDGLQVQQGGSSELDLASATQISQGANGWQIISTTSGTDSPKDLGNNCNEDNDSSSKNRAATTGQYVVTTSNMQNQQVLTGLSGMLPNIQYQVIPQFQTVDGQQLQFATAPTQLSVQQDSSGQLQIIPGTNQQLITNRSGTGNILAAMPNLLQQAVPIQGVNALSGQTQYVTNVPLALNGNITLLPVNSIAASLAPTSQSVTLSNSSSPENSSQPVSVTSISSSSVAATQTSTASFFTNANNYSATTTTSNLGIMNFSTSGSLGTNVQVQRTNSTQNADAVQVAVQQGQQKDIDQNHQHQQILVQPQLVQGGQGSQTFAPQDALQNLQIQALSNAGPIFIRTPTVGPNGQVSWQTIQLQNLQVQNPQAQTIALAPVQGVSLGQTGTSTTLTPIASASLPSGTVTVNAAQLSSVPGLQTINLGALGSGIQVHQLQGLPLTIANATGDHNAHLSLHGTAGDGLADDGTTLEEGDGSPDAQPQQVRRMRREACTCPYCKDSDGRGSGDPGKKKQHICHIPGCGKVYGKTSHLRAHLRWHTGERPFVCSWTYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKAGAPNNVAMDVSAVAIDANASAEGTGGATPSTLIATNMVAMEAICPEGIARLASSGINVMQVADLQSINISGNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQZ4</accession>
    <name>A0A6I9YQZ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="3">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106552858</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013926722.1">
        <property type="nucleotide sequence ID" value="XM_014071247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQZ4"/>
    <dbReference type="GeneID" id="106552858"/>
    <dbReference type="KEGG" id="tsr:106552858"/>
    <dbReference type="OrthoDB" id="547665at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005024">
        <property type="term" value="F:transforming growth factor beta receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF49">
        <property type="entry name" value="ANTI-MUELLERIAN HORMONE TYPE-2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="68"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926722.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="32142" checksum="33D33ED8A7A00A47" modified="2020-10-07" version="1">MDALSKNRSCVSYGSNALTEGLEHLQGLILEDGRNGTIQCPHNQCCFGIWDLNQGQLQAKVQEYIENDDPLELLHEGQLSKLWQGTFGQQPVAIKAFPPLCHQQFASEWAMYSLPLMNHENVGRLVTAGYGGPSEERCGLLVLTLCPLGSLRNFLSHHICSWEISIRLGSSLASGLAFLHEEKREEGLHKPGVAHRDLSSQNVLVREDRTCVITDFGFAMTLPLRHEQSRKGPAEAIVHKVGAQRYVAPEILDENLNLQDVGSALRQADVYSMALVLWEILMRCSALFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR68</accession>
    <name>A0A6I9YR68_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Clathrin heavy chain 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926130.1">
        <property type="nucleotide sequence ID" value="XM_014070655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR68"/>
    <dbReference type="GeneID" id="106552395"/>
    <dbReference type="KEGG" id="tsr:106552395"/>
    <dbReference type="OrthoDB" id="2113814at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045334">
        <property type="term" value="C:clathrin-coated endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015348">
        <property type="entry name" value="Clathrin_H-chain_linker_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022365">
        <property type="entry name" value="Clathrin_H-chain_propeller_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF7">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09268">
        <property type="entry name" value="Clathrin-link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01394">
        <property type="entry name" value="Clathrin_propel"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clathrin heavy chain linker core motif" evidence="1">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926130.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="62348" checksum="23BBB942221783EC" modified="2020-10-07" version="1">MVIKRIVLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDSNNPIRRPISADSAIMNPASKVIALKDAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNTEESTLFCFAVRGQAGGKLHIIEVGAPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNTLFAQGSYSEAAKVAANAPKGILRTPDTIRRFQSVPAQAGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRW3</accession>
    <name>A0A6I9YRW3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Adenylyl cyclase-associated protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106553121</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927042.1">
        <property type="nucleotide sequence ID" value="XM_014071567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRW3"/>
    <dbReference type="GeneID" id="106553121"/>
    <dbReference type="KEGG" id="tsr:106553121"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019933">
        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.330:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.330">
        <property type="entry name" value="Adenylate cyclase-associated CAP, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013992">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036222">
        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF2">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01213">
        <property type="entry name" value="CAP_N-CM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP N-terminal" evidence="3">
        <location>
            <begin position="45"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013927042.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28304" checksum="A7201D1D7C9C56A6" modified="2020-10-07" version="1">MAEVALIERLEKAVIKLESLLSESHRTSGAINGVNGELAPYVEAFDRLMNESVAEFIKNSRILDGDIKTHAEMVHAAFQAQRAFLWLTSRYQEPQQNEVAVLLKPISEKIQQIQTFRERNRGSNMFNHLSAVSESIPALGWITISAKPGPYVKEMNDAATFYTNRVLKDYKHSDLRHIDWVKSFLNIWTELQAYIKEYHTTGLIWSKTGPVASAASSFPAVGNKQGLPPPPPPLPPPPPPPGPPPAIDTENTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS01</accession>
    <name>A0A6I9YS01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Angiotensin-converting enzyme</fullName>
            <ecNumber evidence="13">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ACE2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12 13">Belongs to the peptidase M2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013926936.1">
        <property type="nucleotide sequence ID" value="XM_014071461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS01"/>
    <dbReference type="SMR" id="A0A6I9YS01"/>
    <dbReference type="GeneID" id="106553039"/>
    <dbReference type="KEGG" id="tsr:106553039"/>
    <dbReference type="CTD" id="59272"/>
    <dbReference type="OrthoDB" id="10029630at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008241">
        <property type="term" value="F:peptidyl-dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06461">
        <property type="entry name" value="M2_ACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001548">
        <property type="entry name" value="Peptidase_M2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514:SF24">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01401">
        <property type="entry name" value="Peptidase_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00791">
        <property type="entry name" value="PEPDIPTASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52011">
        <property type="entry name" value="PEPTIDASE_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4 9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 6 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor 2" evidence="11">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="6">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) (complex) asparagine" evidence="6">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; partial" evidence="6">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9 12">
        <location>
            <begin position="154"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-10"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926936.1"/>
        </source>
    </evidence>
    <sequence length="461" mass="53723" checksum="80801BFC5B97D850" modified="2020-10-07" version="1">MKQALIRKPSSRSFTHPAFFDLKGNMLSWLCFTWSLVVLAGAQDVTQQAAEFLKQFDARADDLYYAASIASWNYNTNLTEENAKIMHEKDNIFSKFYEEASKNASMYNVNQITNETIRLQLHLLQNVPTNSSTKDQLDTVLRKMSTMYSTGTVCKQDDPFNCLPLEPGLDDIMENNWSYSERLWAWEGWRADVGKKMRPLYESYVELKNKYARLRGYADYGDYWRANYEVDLPKEYQYQRAQLITDVENTLQQIMPLYKHLHAYVRRHLYKHYGPEFINPEGAIPAHLLGDMWGRFWTNLYPLMVPFPNKTSIDVTSAMVTKKWTVNSIFKAAEQFFTSIGLFPMTDNFWNNSMLEEPKDGRKVVCHPTAWDMGKKDYRIKMCTKINMEDFLTAHHEMGHIEYDMAYSDQPFLLRNGANEGFHEAVGEIMSLSAATPKYLKSLGLLEHTFQEDTEIKHNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS97</accession>
    <name>A0A6I9YS97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box/WD repeat-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">F-box and WD-40 domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">FBXW2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Directly interacts with SKP1 and CUL1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926505.1">
        <property type="nucleotide sequence ID" value="XM_014071030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS97"/>
    <dbReference type="GeneID" id="106552694"/>
    <dbReference type="KEGG" id="tsr:106552694"/>
    <dbReference type="CTD" id="26190"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd22131">
        <property type="entry name" value="F-box_FBXW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000151">
        <property type="entry name" value="F-box/WD repeat-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042627">
        <property type="entry name" value="FBXW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436">
        <property type="entry name" value="F-BOX/WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="10">
        <location>
            <begin position="71"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="161"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="239"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013926505.1"/>
        </source>
    </evidence>
    <sequence length="471" mass="53507" checksum="617DB6E132A46BC6" modified="2020-10-07" version="1">MPARVATAFQGKCFHFLMERKEFEAWLDNLSVTFLSLVDLQKNEALDHLIALSGAAQLKHLSNNLEALLKRDFLKLLPLELSFYLLKWLDPQTLLTCCLVSKQWNKVISACLEVWQGACRNLGWQIDDSLQEALHWKKVYLKAILRMKQLENHQAFETSSLIGHSARVYALYYKDGLLCTGSDDLSAKLWDVSTGQCIYGIQTHTCAAVKFDEQKLVTGSFDNTVACWEWSSGAKTQHFRGHTGAVFSVDYNDELDILVSGSADFTVKVWALSTSFCLNTLTGHTEWVTKVVLQESKVKSLMHSPGDYILLSADKYEIKIWPIGREINCKCLKTLTVSEDRSISLQPRLHFDGKYIVCSSAIGLYQWDFASYDILRVIKPPEFANLSLLGFGDIFALLFDNHYLYIMDLRTEKTISRWPLPEYRKSKRGSSFLAGEMAWLDGLDGENDMGLVFATSMPDHSIHLVLWKEHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSB5</accession>
    <name>A0A6I9YSB5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553239</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927177.1">
        <property type="nucleotide sequence ID" value="XM_014071702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSB5"/>
    <dbReference type="GeneID" id="106553239"/>
    <dbReference type="KEGG" id="tsr:106553239"/>
    <dbReference type="OrthoDB" id="9615015at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0552">Olfaction</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927177.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="35999" checksum="7F9148FB56F29DC2" modified="2020-10-07" version="1">MDKGNDSIITEFILQGFTDNPQMQLVLFFIFLSIYFIAVVGNIGMILLICTNTQLHKPMYYFLGNLSFVDLCCSSTIAPRMLIDLLSESKRISYNACATQLFFYVFFVDIECVLLAVMAYDRYVAICNPLLYSATMTKTLCQQLIAFAYCIGLMDTIVYTSSTFLLTFCRSNLINHFFCDMPPLFVLSCSDTSVNELVLLIIDGFIEACSICMSLISYVYIVVTILRMHSSEGRRKAFSTCASHLAAVGIFHGTIVFMYIRPTSSYSMDQDKWASMFYTVVIPMLNPLIYSLRNKEVKDALIKSVGNVWTFKDRKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSM6</accession>
    <name>A0A6I9YSM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Arachidonate 5-lipoxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ALOX5</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927287.1">
        <property type="nucleotide sequence ID" value="XM_014071812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSM6"/>
    <dbReference type="GeneID" id="106553343"/>
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    <dbReference type="CTD" id="240"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01753">
        <property type="entry name" value="PLAT_LOX"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.60.20:FF:000002">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
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        <property type="entry name" value="LipOase_C"/>
    </dbReference>
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        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
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    </dbReference>
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        <property type="entry name" value="Lipoxygenase"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIPOXYGENASE"/>
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        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
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        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIPOXYGENASE_1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="15">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="16">
        <location>
            <begin position="117"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
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    <feature type="binding site" evidence="11">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
            <position position="80"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="373"/>
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        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="12">
        <location>
            <position position="103"/>
        </location>
    </feature>
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        <location>
            <position position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003974"/>
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        <source>
            <dbReference type="PROSITE" id="PS50095"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_013927287.1"/>
        </source>
    </evidence>
    <sequence length="483" mass="56833" checksum="EF4F5BFCBE33B434" modified="2020-10-07" version="1">MPSYFVTVATGTQWFAGTDDYIYLTLVGLAGTSERHLLDKPFYNDFERGATDTYEVTVTEDLGEIQLIKIEKRKYWFPDDWYLKYIAVKTPVGDYLEFPCYRWITDEKEVVLRDGRAKLFEDEKTQILKLQRRKELEERQKLYKWTEWQPGFPLSIDAKSHKELPRNIQFDSEKGVDFILNYTKAIENLCINRFMHMFQSSWSDFADFEKIFVKISNTISEYVMQHWQEDFMFGYQFLNGCNPVLIKKCIELPKKFPVTTEMVEYSLERNLTLEQEIKQGNIFIVDYEQLEDIDVNKTDPCTVQYLAAPICLLYKNLENKILPIAIQINQAPEPCNPIFLPSDAKYDWLLAKIWVRSSDFHIHQTVTHLLRTHLVSEVFAVAMFRQLPAVHPLFKLLIPHVRFTIAINTKAREQLICEYGLFDKANATGGGGHVQMVQRAMEHLTYSSLCFPEEIKSRHMESHEDIPYYYYRDDGVKVWDAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUH1</accession>
    <name>A0A6I9YUH1_9SAUR</name>
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        <submittedName>
            <fullName evidence="5">Signal-regulatory protein beta-1 isoform 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106554146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014072753.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Ig_V-set"/>
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    <dbReference type="PANTHER" id="PTHR19971">
        <property type="entry name" value="SIGNAL-REGULATORY PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="IGc1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="12"/>
            <end position="103"/>
        </location>
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            <end position="226"/>
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            <end position="326"/>
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    </feature>
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        <location>
            <begin position="346"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
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        <source>
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            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928228.1"/>
        </source>
    </evidence>
    <sequence length="451" mass="48941" checksum="97E7B45763C31812" modified="2020-10-07" version="1">MAVIQEWSDHTPEVVTGQNVEVAQLPESISVKAGETITLKCTVAGGNLPGGVRWYKGTDRNQPVYSDKQGTSNQGVRVIPGSNTDFSIKIQNVRPEDAGTYYCGKVKAGLQESVLALGNGTLVSVIAMPSQPVIIGPTGRITAGSRASFSCSTGGFFPQEITVSWLKDGKKIPAEILNSNEKQSISYRAESTVNIQLEQGDMKSQLICQIEHTSLDGQGPLQETFTLGDVLRVPPKVRLETSPPSPVPLNTTVTVTCNAESFYPDGETLELFAKDAPTRRGTVGPKSSNPDGTFSLKSSLEMMASEERNSSVFLCQVQHDSQPLVNTIITLFITQQDEMKGVGGRPNSNPEAVTITAGKTLILICILTQGYVPSSVEWYKDLGNDQKIIYRESDKFSRWERFVPGSPTDFSVRLKNIGPKDSGTYCCSMVKEIPQGRKDIRAKTVVSVIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUK2</accession>
    <name>A0A6I9YUK2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-amino-acid oxidase</fullName>
            <ecNumber evidence="4">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106553926</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="4"/>
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        <property type="nucleotide sequence ID" value="XM_014072512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUK2"/>
    <dbReference type="GeneID" id="106553926"/>
    <dbReference type="KEGG" id="tsr:106553926"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000450">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF355">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026887223" description="L-amino-acid oxidase" evidence="12">
        <location>
            <begin position="26"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="13">
        <location>
            <begin position="68"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927987.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="21154" checksum="E77FC13BA9532BF7" modified="2020-10-07" version="1">MVMGLQGCCGQFVFSLLFLAALGSCADDRSPLEECFREPDYEEFLEIAKNGLKPTSNPKHVVVVGAGMAGLSAAYVLAGAGHKVTVLEASERAGGRVTTYRNEIEGWYANLGPMRIPEGHRIIREYIRKFGLKLNEFFQENDNAWYFIKNIRKRVWEVKEDPSVLKYPVDPSEEGKSASQLYRESLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUS4</accession>
    <name>A0A6I9YUS4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-lysine(27) N-trimethyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.356</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">EZH1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60292"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.356"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.356" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013928343.1">
        <property type="nucleotide sequence ID" value="XM_014072868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUS4"/>
    <dbReference type="GeneID" id="106554239"/>
    <dbReference type="KEGG" id="tsr:106554239"/>
    <dbReference type="CTD" id="2145"/>
    <dbReference type="OrthoDB" id="6141102at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140951">
        <property type="term" value="F:histone H3K27 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19217">
        <property type="entry name" value="SET_EZH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000001">
        <property type="entry name" value="Putative histone-lysine N-methyltransferase EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026489">
        <property type="entry name" value="CXC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048358">
        <property type="entry name" value="EZH1/2_MCSS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021654">
        <property type="entry name" value="EZH1/EZH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044438">
        <property type="entry name" value="EZH1_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041343">
        <property type="entry name" value="PRC2_HTH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041355">
        <property type="entry name" value="Pre-SET_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EZH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21358">
        <property type="entry name" value="Ezh2_MCSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11616">
        <property type="entry name" value="EZH2_WD-Binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18118">
        <property type="entry name" value="PRC2_HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18264">
        <property type="entry name" value="preSET_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51633">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CXC" evidence="14">
        <location>
            <begin position="584"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="13">
        <location>
            <begin position="693"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="431"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="448"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="488"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013928343.1"/>
        </source>
    </evidence>
    <sequence length="827" mass="94442" checksum="C7FB95C5A576A2A1" modified="2020-10-07" version="1">MRVQEGSSAFSVGHLCTMSKEKVEIPGPPTSKCILYWKRKVKSEYMRLRQLKRLQANLGAKGFYRRNMQQHLPSVSEGEGKNFIWGHLEFPSTLTFHQSSGQCCFQHKDEANSSGLTENLFVSNFTKIQEKINILNDDWKKLRVQPIQSMKLVGGNPFLKQCTVESIFPTLSTQTLFMRTLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELINNYEGKVHGEEELLPGSVLISDAVFLELVDALNQYSEEEDEGHNIVEGKQEALKEDLPVTRKRKRLTMESNKKSSKKQFPNDMIFTALSSMFPENGYPEDMKERYRELTEEESDMNVLPPQCTPNIDGPNAKSVQREQALHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNMETKIETEPCGSDCFLWLEGAKEFAMMHNPRSKYAGRRRRRNPTPSATASSSATASTSAEAKEGDSDRDTGNDWASSSSEANSRCQTPTKPKMSPSSSQPFMSDVPLEPVEWTGAEETLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAVKESLITKLPVNELLNPSQKKKRKHRLWAAHCRKIQLKKDNSSTQVFNYQPCDHPDHPCDSSCPCIMTQNFCEKFCQCNPDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDSKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERESDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVR6</accession>
    <name>A0A6I9YVR6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM98B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554546</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM98 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928723.1">
        <property type="nucleotide sequence ID" value="XM_014073248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVR6"/>
    <dbReference type="GeneID" id="106554546"/>
    <dbReference type="KEGG" id="tsr:106554546"/>
    <dbReference type="OrthoDB" id="512356at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018797">
        <property type="entry name" value="FAM98"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31353">
        <property type="entry name" value="FAM98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31353:SF10">
        <property type="entry name" value="PROTEIN FAM98C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10239">
        <property type="entry name" value="DUF2465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928723.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="36651" checksum="E7A152A253B3270A" modified="2020-10-07" version="1">MSPQEFPSVKTFTEVAEAGVSSPRFMTLCSWLVSELRTICRLEEDVSPVQGPEDAETFQIEISGLLAELHCPYPSLTTGDVMARLRTRDNCLQLLYFLSSELLAARLQARKTLQSSKQGDRKNEAVRELHRICQALGMPEPDPASSVAQVMDDIGSQVSEALAAQGPEPDRTAPLLKAPLTSEQWKALDEINQLLGAEYQCRRHMMLTRFDVTVASFYWSERAKDRSAAMSEAFQPLRKSLPEDSQLSLAHLLAAREDFSRIVKTSSGALRSKTSCAVNKILMTGSVPDRGGRPNEIEAPMPTWEKRREGGGRGGGGGGGQRWSKRGNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVX3</accession>
    <name>A0A6I9YVX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Cortexin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">CTXN2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928174.1">
        <property type="nucleotide sequence ID" value="XM_014072699.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928175.1">
        <property type="nucleotide sequence ID" value="XM_014072700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVX3"/>
    <dbReference type="GeneID" id="106554099"/>
    <dbReference type="KEGG" id="tsr:106554099"/>
    <dbReference type="CTD" id="399697"/>
    <dbReference type="OrthoDB" id="9947540at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020066">
        <property type="entry name" value="Cortexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16736">
        <property type="entry name" value="CORTEXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16736:SF2">
        <property type="entry name" value="CORTEXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11057">
        <property type="entry name" value="Cortexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928175.1"/>
        </source>
    </evidence>
    <sequence length="81" mass="8884" checksum="F5B1572317C1FE64" modified="2020-10-07" version="1">MSSSYCNNVSVTMSVNEVSSFPLTLEQKTGFAFVGILCVFLGLLIIRCFKILLDPYSSMPSSTWEGEVEGLDKGTFEYALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXU2</accession>
    <name>A0A6I9YXU2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450 3A</fullName>
            <ecNumber evidence="12">1.14.14.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106554576</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17149"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:142491"/>
            <dbReference type="EC" id="1.14.14.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Microsome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013928761.1">
        <property type="nucleotide sequence ID" value="XM_014073286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXU2"/>
    <dbReference type="GeneID" id="106554576"/>
    <dbReference type="KEGG" id="tsr:106554576"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050649">
        <property type="term" value="F:testosterone 6-beta-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070989">
        <property type="term" value="P:oxidative demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008072">
        <property type="entry name" value="Cyt_P450_E_CYP3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050705">
        <property type="entry name" value="Cytochrome_P450_3A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24302:SF38">
        <property type="entry name" value="CYTOCHROME P450 3A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24302">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01689">
        <property type="entry name" value="EP450IICYP3A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0492">Microsome</keyword>
    <keyword evidence="10 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013928761.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24417" checksum="D0095DB91EDC44DA" modified="2020-10-07" version="1">MFPIIQNYAKNLLVLLQKKADRDELVNVKQTFGAYSMDVITSTSFGVNMDSINNPKDPFVQQVQKLLSADFFSPIFILLYVAPWIKPLLEKMNISVFSNESIDFFERSITKLKETRAQEGEKRRVDFLQLMIESQDSLTSHEMNGVNHTYKGNNLKSSSTVTSFKPEPAFIAKSASVGINKTMRARQTSISVKVRGKALRQFRNILKLVGLRETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY53</accession>
    <name>A0A6I9YY53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 66</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106555308</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929608.1">
        <property type="nucleotide sequence ID" value="XM_014074133.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555308"/>
    <dbReference type="KEGG" id="tsr:106555308"/>
    <dbReference type="OrthoDB" id="9038689at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001747">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002093">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390:SF159">
        <property type="entry name" value="GROWTH FACTOR INDEPENDENT 1 TRANSCRIPTIONAL REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929608.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="30118" checksum="D05FC72D9B5D91F4" modified="2020-10-07" version="1">MQKFGIQMELQTHDRNQSNDWNQESSSSTDAPMQDFLAQQEKPYNFLTTQKVIDKKEKLYKCLECGKCFRTSRQLTSHERIHTGEKLFKCRVCGNTFRTYGNLIFHKRIHSGDKPYKCMECGKTFAHSHYLASHKKIHTGEKPYKCMECGKAFTYSNGLTIHKMIHGGKKPFKCLECGKTFARQNHLISHKMTHTGEKPYKCVDCGKTFVRRDNLISHKIIHTGGKPYKCMQCGKTFTYSSGLSVHKKLHTGDKPYKCMXG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYT8</accession>
    <name>A0A6I9YYT8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Olfactomedin-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106555223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929482.1">
        <property type="nucleotide sequence ID" value="XM_014074007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYT8"/>
    <dbReference type="GeneID" id="106555223"/>
    <dbReference type="KEGG" id="tsr:106555223"/>
    <dbReference type="OrthoDB" id="8626508at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF7">
        <property type="entry name" value="OLFACTOMEDIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="5">
        <location>
            <begin position="89"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929482.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="40638" checksum="5746A5DD16EECE5A" modified="2020-10-07" version="1">MNLMRICEADDCGRGITIVRDSQAVVELAADSDSEEIKNLSVMVNGLEVLDKNNVLAVRREVAALRAKLEECEKARNQTVEHPYFPPGNCGHGPITNISQPYVIQLNWRGFSYKYGAWGRYSSPLSPGKEMYWVAPLNPEGRYLEYYRLHDSYDNLLLFKYSKEYMYRYGEGSGTAVHNNHMYINIYSSRDMGRIDLATNKLVLRKPLPNAVYNNRFSYAGVAWQDLDFAVDENGLWVIYSTEASQGNIVISKINETTLDVLNTWQTTQYKPAVSNAFIICGVLYATRPLNTRKEEIFYSFDINTGKEGRLSIIVDKVLETIQSINYSPIDRRLYVYNDGYLVRYGLNFEPLPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYU9</accession>
    <name>A0A6I9YYU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20 27">Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN</fullName>
            <ecNumber evidence="6 27">3.1.3.16</ecNumber>
            <ecNumber evidence="5 27">3.1.3.48</ecNumber>
            <ecNumber evidence="4 27">3.1.3.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Phosphatase and tensin homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">PTEN</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83420"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:43561"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43552"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83416"/>
            <dbReference type="ChEBI" id="CHEBI:83419"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:43553"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,4,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57627"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:77144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77155"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:77156"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:10685"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.67"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:25018"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Nucleus</location>
            <location evidence="27">PML body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 27">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6 27"/>
    <dbReference type="EC" id="3.1.3.48" evidence="5 27"/>
    <dbReference type="EC" id="3.1.3.67" evidence="4 27"/>
    <dbReference type="RefSeq" id="XP_013929888.1">
        <property type="nucleotide sequence ID" value="XM_014074413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYU9"/>
    <dbReference type="GeneID" id="106555545"/>
    <dbReference type="KEGG" id="tsr:106555545"/>
    <dbReference type="CTD" id="5728"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
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    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 27" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 27" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 27" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 27" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15 27" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12 27" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="31">
        <location>
            <begin position="14"/>
            <end position="185"/>
        </location>
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            <begin position="384"/>
            <end position="394"/>
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            <position position="124"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034268"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034338"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_013929888.1"/>
        </source>
    </evidence>
    <sequence length="404" mass="47299" checksum="892EAC7BDA2FD953" modified="2020-10-07" version="1">MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDATKFNCRVAQYPFEDHNPPQLELIKPFCEDLDRWLSEDGNHVAAIHCKAGKGRTGVMICAYLLHRGRFQKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPHFVVYQLKVKIFTSSSGPTKREDKYMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGLEENSDKVENGSLLGDQELDGILSTERSDNDKEYLILTLTKNDLDKANKDKANRYFSPNFRVKLFFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZ73</accession>
    <name>A0A6I9YZ73_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cadherin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106554957</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014073706.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106554957"/>
    <dbReference type="KEGG" id="tsr:106554957"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000027">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF81">
        <property type="entry name" value="CADHERIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929181.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="23825" checksum="DC56264CBB42A079" modified="2020-10-07" version="1">MRYSKLSDPANWLNINATNGQIITAAVLDRESAYVKNNVYEATFLATDNGIPPASGTGTLQIYLIDINDNAPDLQPKEAQICERPNLNVINITAADADIDPNVGPFVFELPSVPSAIRKNWTITRLNGKPSGHHLALFVAWHGQKPNITENLESGKKRLNFEVGSKGHLSNLLHHQTDLSIIPPLYLDRTFNPWFYLLEAQYRLSGTEWFNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0A5</accession>
    <name>A0A6I9Z0A5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2 9">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">POLRMT</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 9"/>
    <dbReference type="RefSeq" id="XP_013929285.1">
        <property type="nucleotide sequence ID" value="XM_014073810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0A5"/>
    <dbReference type="GeneID" id="106555042"/>
    <dbReference type="KEGG" id="tsr:106555042"/>
    <dbReference type="CTD" id="5442"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000031">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="10">
        <location>
            <begin position="75"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013929285.1"/>
        </source>
    </evidence>
    <sequence length="792" mass="90693" checksum="077EF1B885638582" modified="2020-10-07" version="1">MFSLLMCHYAKQGALKKIGWLFCMLEENDLKPNLDCYMAVLNCMGRIKTEPRIISRCIEQLESDGFSLQSFFKDSTYEDNEVKMILAAIQRVKPDFQPEFFSPPTTTIPLLDKFYKKVKFESLCYLRGFILPSFQTVFSLPALGECVTVLAKELGTRIYNKYTILRKSCTISEVNFEKTYDSYVQLLARNNKQVNGFLPREYWEKLRAEVGYSPSLISKQAVWPPSLLIQLGFQLIELMVQTLKLDHSLLPSLPETSVIPVLYHVYSFRGTKQIGFIKPHYLLIRILQEASETTLTFDSFVLPMQCPPVPWVSSHFGAYTLSPVKLMRCQDGVIQHDLLLDECPHDHLCLVLDALNFLGNCPWKVNQPVLDVIISIFNDKGDEKLDVPPPPSWEAKELARQLAESAPMSRMALKWRMAQCRKKTRETYGLRMDMLYKLSIAKHMKDEVFWFPHNLDFRGRTYPCPPHFNHLGGDFTRGILLFAEGKPLGPRGLDWLKIHLVNLTGLRKKHSLKERLAYADQIMADILDSADNPLTGKRWWMDTDEPWQVLACSMEIAKAVRSPDPSKYISHFPVHQDGSCNGLQHYAALGRDMLGAFSVNLTPSALPQDVYTSVAQQVELSRKNDAEKGVKIAQVLDGYVTRKVVKQTVMTVVYGVTQYGGRLQIEKQLKEIENFPKEDVWQASKYLVQLVFQSLSEMFSGSREIQNWLTESAWLISKTGQTVEWVTPLGLPIIQPYHNKKSILVKGDLQSIYLQSNYNVDQKPNTRKQKNAFPPNFIHSLDSSHMMLTALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0L4</accession>
    <name>A0A6I9Z0L4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Microfibril-associated glycoprotein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MFAP4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Initiates complement activation and/or interferes in platelet aggregation and/or blood coagulation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ficolin lectin family. Veficolin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929621.1">
        <property type="nucleotide sequence ID" value="XM_014074146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0L4"/>
    <dbReference type="GeneID" id="106555320"/>
    <dbReference type="KEGG" id="tsr:106555320"/>
    <dbReference type="CTD" id="4239"/>
    <dbReference type="OrthoDB" id="9990035at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048251">
        <property type="term" value="P:elastic fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF225">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1216">Complement system impairing toxin</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="6" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026760036" evidence="7">
        <location>
            <begin position="28"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="39"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013929621.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29676" checksum="F11F422B246D4D1E" modified="2020-10-07" version="1">MKLSQASSFLPISLFLLLLLQLPTLQGQAINSAQQSQPCGEDSFPLDCEDIYDQGSETDGVYLIYPAGPSVPVPVYCDMTTDDGKWTVFQKRFNGSISFFRGWNDYKFGFGKADGEYWLGLQNIHLLTLKQKYELRVEMDDFDNNTAFAKYADFSLSPNAISAEEDGYTLHVSGFTDGGAGDSLSYHSGQKFSTFDRDQDLYVQNCAALSSGAWWFKSCHFSNLNGFYLGGRHLSYANGINWYQWKGFYYSLKRSEMKIRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1A4</accession>
    <name>A0A6I9Z1A4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phospholipase D</fullName>
            <ecNumber evidence="1">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106555508</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.1.4.4" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013929851.1">
        <property type="nucleotide sequence ID" value="XM_014074376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1A4"/>
    <dbReference type="GeneID" id="106555508"/>
    <dbReference type="KEGG" id="tsr:106555508"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032534">
        <property type="term" value="P:regulation of microvillus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000009">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000048">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF57">
        <property type="entry name" value="PHOSPHOLIPASE D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="6">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="6">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013929851.1"/>
        </source>
    </evidence>
    <sequence length="563" mass="64309" checksum="44558F6217CDC972" modified="2020-10-07" version="1">MYVDTLMKVITILFAGGLSRLTAWIGASAYIHLRKCSKVDNSSTEKSSLVCFSAKLSTFFYRLSPEIFMKRPVVEGNRWRLDCILRRKAQQGVRIFVILYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRITANKSESSVNLSSLAETVQPQVETPVTTPLQGCSDDAQEISRMKGVGRTKKFVRFSIYKQLHRHTMHRADSVSSVDSDSCKGSVRSLKTGVGELLGETRFWHGKDYCNFVFKDWIQLDKPFADFIDRYTMPRMPWHDIASVVHGKAARDVARHFIQRWNFTKIMKSKYRSLSYPFLLPKSQRTADELNYQVPEAVNAKVQVLRSAADWSAGIKYHEESIHSAYVSVIENSKYYIYIENQFFISCADDKVVSNRIGDAIAHRILKAYRENKRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENSILGQLKAEIGNQWINYISFCGLRNYAELEGKLITELIYVHSKLLIADDNTVIIGSANINDRSMLGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2R0</accession>
    <name>A0A6I9Z2R0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TBC1 domain family member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106555773</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930155.1">
        <property type="nucleotide sequence ID" value="XM_014074680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2R0"/>
    <dbReference type="GeneID" id="106555773"/>
    <dbReference type="KEGG" id="tsr:106555773"/>
    <dbReference type="OrthoDB" id="295078at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930155.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="13659" checksum="5A67B7A7D9ACD81B" modified="2020-10-07" version="1">MDISKQLQAYEVEYYVLQDELTDSSLNDNQRMDKLEKANSNLRKQNFDLLEELQVANGKIQNLEAMIEVLLNNEGKLKQTIVTLEREGKARIETLTELHTQPANSGGKTLPSEPGRTNAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3X3</accession>
    <name>A0A6I9Z3X3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">F-box only protein 24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FBXO24</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930666.1">
        <property type="nucleotide sequence ID" value="XM_014075191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3X3"/>
    <dbReference type="GeneID" id="106556200"/>
    <dbReference type="KEGG" id="tsr:106556200"/>
    <dbReference type="CTD" id="26261"/>
    <dbReference type="OrthoDB" id="101791at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd22098">
        <property type="entry name" value="F-box_FBXO24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052866">
        <property type="entry name" value="F-box_protein_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47004">
        <property type="entry name" value="F-BOX ONLY PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47004:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="399"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930666.1"/>
        </source>
    </evidence>
    <sequence length="591" mass="66813" checksum="FFA8833F602C2D29" modified="2020-10-07" version="1">MFLDPRRSPGRSRIRGLLAPLFCLQQPKKKRRSSSTEATEDKKARGESASIQLFPPELLYYIISFLPVRDLVALGQTCRYFYEVCDSEGVWQRICRRLSPRLRDENSGGSRPWKRSAILNYTKGLYFQMFSGRRHFVSKTVAPLLSHGYRKFLPTKDHVFILDYNGTLFFLKNALVSSTMGHIQWRRACRYMVLCRSAKDFATDPRNDTVYRKYLYVLATREQLPSQALGRSEGSGPAPSSRLCDCVEVYLQSSGQRVFKMTFHFSMKFRQIVLVGQETERSLLLLTEEGKIYSLVVNETQLDQPRSYTVQLALRKVSKCLPHISVRHMRSNQSSAVYLTGEGTVYFEVHSPGVYRDLFGTLHAFDPLDQQMPLALSLPAKVLSCALGYNHLGLVDEFGRIFMQGNNRYGQLGTGDKIDRGEPAQVHYLKWPLDLWCGLNHTLVLSQSGDFSKDLMGCGCGAGGRLPGWPKGSASFVRLQIKVPPCARHICSTRECLYMLSSHDIEEAPAYRELPASKAAVAPGDSEALAARTCEEYLSQLHKCPTLEGRMAKMKEAVGSMPLLTFQKDFFWEALDMIQRAAELREAPPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4L3</accession>
    <name>A0A6I9Z4L3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mitochondrial inner membrane protein OXA1L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106556407</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930911.1">
        <property type="nucleotide sequence ID" value="XM_014075436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4L3"/>
    <dbReference type="GeneID" id="106556407"/>
    <dbReference type="KEGG" id="tsr:106556407"/>
    <dbReference type="CTD" id="5018"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF66">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN OXA1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="12">
        <location>
            <begin position="15"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="260"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013930911.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="33741" checksum="382405911A9021C7" modified="2020-10-07" version="1">MKILEALHLDVGLPWWGAIVAGTVFIRCLVFPLIVKSQRDAVKLNNHVPQITALTTRISEAKKAGNRLLFSQAVMDLEHYQKTHNVNPLRGLIVPFVQAPIFISFFFALRKMAECPVPSMQTGGLLWFTDLTVADPYYILPVMATVTMWIVMEVGMQSGPPNPNLELMKKMFRVMPVFFLPFIISFPTAIFTYWLTSNLFSIMQILLFRLPAARAFFHIPKSLEHNPQSLPAQEDFLKSIKEGWQNVQMAHQLDERKKYFKKQADSTHKGSLQQTFSQRTTQHPSKPSPQKRELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4Q9</accession>
    <name>A0A6I9Z4Q9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106557049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931637.1">
        <property type="nucleotide sequence ID" value="XM_014076162.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106557049"/>
    <dbReference type="KEGG" id="tsr:106557049"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="34"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931637.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="20831" checksum="B0433EB18C0230E5" modified="2020-10-07" version="1">MAVLGEKYSDLLQCLQYQFYQIPGTCLLPEVTQQDQGRICVVIDLDETLVHSSFKVKPLILSLSFYAQSSPFISFFLSSGXYADPVTDLLDKCGVFRTRLFRESCVFHQGCYVKDLSRLGRDLRKTLILDNSPASYIFHPENAVPVQSWFDDMTDTELLNLIPVFEELSGAEDVYTSLGQLRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5G7</accession>
    <name>A0A6I9Z5G7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipase maturation factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556645</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931100.1">
        <property type="nucleotide sequence ID" value="XM_014075625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5G7"/>
    <dbReference type="GeneID" id="106556645"/>
    <dbReference type="KEGG" id="tsr:106556645"/>
    <dbReference type="OrthoDB" id="434126at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009613">
        <property type="entry name" value="LMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057433">
        <property type="entry name" value="LMF1/2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463">
        <property type="entry name" value="LIPASE MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463:SF10">
        <property type="entry name" value="LIPASE MATURATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25179">
        <property type="entry name" value="LMF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase maturation factor 1/2 C-terminal" evidence="2">
        <location>
            <begin position="42"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931100.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="12794" checksum="38BC998BD94CB5A4" modified="2020-10-07" version="1">SYIRKAVNVSFGALIIFLSIPVVLNLISSQQIMNTSYNPLRIVNTYGAFGSVTKERTEVIIQGTSSSDPNDPAAVWEEYEFKCKPGNLQRRPCLISPYHYRLDWLMWFAAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z633</accession>
    <name>A0A6I9Z633_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15 16">Zinc finger and SCAN domain-containing protein 23-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">LOC106557412</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013932131.1">
        <property type="nucleotide sequence ID" value="XM_014076656.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932132.1">
        <property type="nucleotide sequence ID" value="XM_014076657.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932133.1">
        <property type="nucleotide sequence ID" value="XM_014076658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z633"/>
    <dbReference type="GeneID" id="106557412"/>
    <dbReference type="KEGG" id="tsr:106557412"/>
    <dbReference type="OrthoDB" id="9027221at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
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    </dbReference>
    <dbReference type="SMART" id="SM00349">
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    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="11">
        <location>
            <begin position="252"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="12">
        <location>
            <begin position="346"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="539"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="595"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="418"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
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            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013932131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013932132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013932133.1"/>
        </source>
    </evidence>
    <sequence length="636" mass="72789" checksum="D0E43F93EBB63FBF" modified="2020-10-07" version="1">MEKSSPPVGTIKEFLSCSPSWKDRVDLNGTVVKPQYWEVWQQVLLPSPQHKETDASPPKIMLDDIQVCVVSSENSSDSFKEKEANTEGKVGIDCVITIEEEIEVQGKSSPESRIELRDDIKICTVPELQDEAPLMATDGGKTKDFTKPVKGRGLSKVEKATQTEVTQMEMAKICEDYIQKAEGSKDTLRGNHQANVISQNKPRDSEEGMIHLTEPDRIGELEEAACNNLTAGEEEEVTSILKEGGIAMESARQSFRWYCYPEGEGPRKAYRQLLDLCHHWLKPERRSKEQILELLVLEQFLATLPKEIQNWVWQQHPESCGQAVELVENFLTGLSLLERHGKKALVTFEEVSMYFSEEEWRLLDESQRKIFTKVMMENYENVRELGFPTNNPDLVFKMGRNKNNELYMDKIQNLEEGASPEVTGEPEFSNEKEKLATRTQKDMKDVLPSTPTSPRPLKSCDATVKGHSPRRQRARSLLQQTMPGFACPDCGKKFFSNSDMARHQWHHARKQSYQHGSCPKSFAKPSQLAQQQHRQPGECQCSKCGKRFTDDFGLARHQAIHSAKRPFHRHHRGFYFNYNLRQNQNQPATTADSLFHCPDCGRQFTRRCNLIQHSRIHQWQPQGKQEVLLYPGGQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X082</accession>
    <name>A0A6I9X082_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein TGIF2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TGIF2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TALE/TGIF homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908639.1">
        <property type="nucleotide sequence ID" value="XM_014053164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X082"/>
    <dbReference type="GeneID" id="106538628"/>
    <dbReference type="KEGG" id="tsr:106538628"/>
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    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TGFB-induced factor homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="48"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="50"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
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        <location>
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            <end position="245"/>
        </location>
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        <location>
            <begin position="266"/>
            <end position="288"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013908639.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="31379" checksum="51A05825B296E192" modified="2020-10-07" version="1">MNQSSEETWKKHCRIHGLNQDRSANSSTMSDSDFGEDDGSISQDPSQSGKRKRRGNLPKESVKILREWLYEHRFNAYPSEQEKLSLSGQTSLSVLQICNWFINARRRLLPDMLLKDGKDPNQFTISRRGTKASDVALSRGAAAGSGLLMVPPAAAAATTSKVLSVSVCPPVICQTSRPLAGNLTVMTGDLEKRSMLQRIELESHTKQSQLSATGNTLALLTRADASSPTSGLFNTPPPTPPELFGEDFSSFQLLVEVALQRAAELDSQKLSGQLPPSPQRESSPAKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5G2</accession>
    <name>A0A6I9X5G2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Target of rapamycin complex 2 subunit MAPKAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MAPKAP1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909501.1">
        <property type="nucleotide sequence ID" value="XM_014054026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5G2"/>
    <dbReference type="GeneID" id="106539265"/>
    <dbReference type="KEGG" id="tsr:106539265"/>
    <dbReference type="CTD" id="79109"/>
    <dbReference type="OrthoDB" id="241990at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032679">
        <property type="entry name" value="Sin1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05422">
        <property type="entry name" value="SIN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sin1 N-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Sin1 N-terminal" evidence="2">
        <location>
            <begin position="58"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013909501.1"/>
        </source>
    </evidence>
    <sequence length="114" mass="12765" checksum="BC323757FBDE70EB" modified="2020-10-07" version="1">MAFLDNPAIILAHIRQSHVTSDDTGMCEMVLIDHDVDLERFHPASANGDDSSEVPNSNGEPQGYVYSQSVDITSSWDFGIRRRSNTETGAAQKREAESDQVQKRAVEREKCHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6L5</accession>
    <name>A0A6I9X6L5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLCO4C1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911099.1">
        <property type="nucleotide sequence ID" value="XM_014055624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6L5"/>
    <dbReference type="GeneID" id="106540501"/>
    <dbReference type="KEGG" id="tsr:106540501"/>
    <dbReference type="CTD" id="353189"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF160">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="536"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="624"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="63"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="454"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013911099.1"/>
        </source>
    </evidence>
    <sequence length="668" mass="72971" checksum="675DFB191AE53603" modified="2020-10-07" version="1">MNKTGVDNPAFQASNPDLLDAQLPPATPSYLQSPSFDPDDQLCGIGRCTPRALQVCNNVKGYLVFYSLLSVFQGIIVNGLINVSISTIEKRYDLSSSLTGVISAGYDISFSLLCLFISFYGERGHKPRWLALSSFLIAVGALVFSLPHFTSGPYTYGEKAEDLCPVPLSGGPTRPCRRGIKSKLSNYFYVFLLGQFLLGAGGTPLYTLGTTFIDDSVPKHQASVYIGIGYAMSLLGPAIGYVLGGQLLNVYVDFNRNISVDLTPEDPRWVGAWWIGFVICSFSTCLLVAPFSYFPKHLPGTKKIRAEKISEAHHDGKQTCEYTGRSVTDFLMTLWMLLRNPVLMFLVIAAALEALVATGFATFLPKFIENQFGFTSSYSATLGGLVLIPGAAIGQIISGTLISKLQMNCKTIIKFIIATCTLALLLNMVFAFANCGNEPFAGVSETYNGTGMLSNLTAPCNTHCRCLRSDFLPICGADEVQYFSPCYAGCTAVVPQKHGRHYSNCSCVGNPILEIENTFHGATSGKCKSKCTVLPWFLSFFFFAVVFTFMAVTPTTVAILRCVPENHRSFALGVHMLFLRILGTIPGPILFGVSIDHSCTLWSIDRCGRKGACWTYNNSKMAYLLFGISACSKVISLIFTITSYMLYKPPPDIADLKRQDQTISSVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X864</accession>
    <name>A0A6I9X864_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cyclin-T2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CCNT2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910511.1">
        <property type="nucleotide sequence ID" value="XM_014055036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X864"/>
    <dbReference type="GeneID" id="106540051"/>
    <dbReference type="KEGG" id="tsr:106540051"/>
    <dbReference type="CTD" id="905"/>
    <dbReference type="OrthoDB" id="25002at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20596">
        <property type="entry name" value="CYCLIN_CCNT2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20598">
        <property type="entry name" value="CYCLIN_CCNT2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000004">
        <property type="entry name" value="Cyclin T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000009">
        <property type="entry name" value="cyclin-T2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047321">
        <property type="entry name" value="CYCLIN_CCNT2_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047322">
        <property type="entry name" value="CYCLIN_CCNT2_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21797">
        <property type="entry name" value="CycT2-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 12" id="KW-0195">Cyclin</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="14">
        <location>
            <begin position="59"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="14">
        <location>
            <begin position="171"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="467"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="525"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="598"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="634"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910511.1"/>
        </source>
    </evidence>
    <sequence length="725" mass="80005" checksum="A907E41EA6AA49FF" modified="2020-10-07" version="1">MAAALSSGPSASSGSRGPGGGGLGSSRWFFSRERLENTPSRHCGVEADKELSYRQQAANLIQDMGQRLNVSQLTINTAIVYMHRFYMHHSFTKFNRNIISPTALFLAAKVEEQPRKLEHVIKVAHACLHPLEPQLDTKSDAYLQQAQELVILETIMLQTLGFEITIEHPHTDVVKCTQLVRASKDLAQTSYFMATNSLHLTTFCLQYKPTVIACVCIHLACKWSNWEIPVSTDGKHWWEYVDASVTLELLDELTHEFLQILEKTPSRLKRIRNWRANQAARKPKGDGQASENSLLSSSLVQNSILVDAVAGVSANASFQKPSTSTFPAPVPLNSGNLSVQDSQDNLAILAAGMPSTSYNLSTHQDWPQHQEQTRTEQLYSQKQETSLPGAQFNINYPSSSSLQLHSGLHHRADKLSEYSIAHKAGNKHGQLVSGPTIMPQKMSLDKYREKRKLETLELDIREHYLGSEPPYKKHTQTSNSSSVASPIKMKIPIGGVEKPEKYMPDKKDKSGSLKLRIPIPPTEKNTTKEDLKMKIKVSSSERHSSSDEGSGKSKHSSPHVSKEKHKEHSMNRHHGGSHKHSGSSKHGSDGLTPAVLRSPVGLSSDGSVSSSGSSRKKLFSNDASHNHHSKMSKSSKSSGSSSSSSSVKQYVSSLNSVFNLPLPPPPHVTYQVGYGHLSTLVKLDKNPVENGPDASHEYSTNSQHMDYKDTFDMLDSLLSAQGMNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8D8</accession>
    <name>A0A6I9X8D8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aspartate aminotransferase</fullName>
            <ecNumber evidence="14">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">GOT1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2S)-2-aminobutanoate + 2-oxoglutarate = 2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:70223"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:70225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-sulfino-L-alanine + 2-oxoglutarate = 3-sulfinopyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:70295"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="ChEBI" id="CHEBI:140699"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:70297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:17441"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57678"/>
            <dbReference type="EC" id="2.6.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:17442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013907588.1">
        <property type="nucleotide sequence ID" value="XM_014052113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8D8"/>
    <dbReference type="GeneID" id="106537844"/>
    <dbReference type="KEGG" id="tsr:106537844"/>
    <dbReference type="CTD" id="2805"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047801">
        <property type="term" value="F:L-cysteine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000044">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF3">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 14 17" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="15">
        <location>
            <begin position="34"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013907588.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="46577" checksum="213B406BEDE6D815" modified="2020-10-07" version="1">MAPVPGSVFADVPKAAPVAVFQLTEDFRADEDPRKVNLGVGAYRTNEGQPWVLPVVRKVEMMIAKNTNLNHEYLPILGLPEFRANSSRIALGEDSPAIKENRVGGVQSLGGTGALRIGAEFLRRWYNGTNNTATPIYISDPSWENHRSVFMDAGFKDIRNYRYWDAANRGLDIKGFLSDLESAPEFSIFILHACAHNPTGTDPTQEQWKQIAAVMKRRFLFPFFDSAYQGFASGCLDRDAWAVRFFVSEGFELFCAQSFSKNFGLYNERVGNLSVVAKDADNMKRALSQMEKIVRTTWSNPPSQGARIVATTLTTPELFAEWKDNVKTMADRVLQMRAELRSRLEALSTPGTWHHITEQIGMFSFTGLNIKQVQYLIKEKHIYLMSSGRINMCGLTTKNLDYVAKSIYEAVTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8P9</accession>
    <name>A0A6I9X8P9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Numb-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NUMBL</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in the process of neurogenesis.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911929.1">
        <property type="nucleotide sequence ID" value="XM_014056454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8P9"/>
    <dbReference type="GeneID" id="106541115"/>
    <dbReference type="KEGG" id="tsr:106541115"/>
    <dbReference type="CTD" id="9253"/>
    <dbReference type="OrthoDB" id="10070446at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050769">
        <property type="term" value="P:positive regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01268">
        <property type="entry name" value="PTB_Numb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000031">
        <property type="entry name" value="protein numb isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016698">
        <property type="entry name" value="Numb/numb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010449">
        <property type="entry name" value="Numb_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368">
        <property type="entry name" value="NUMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368:SF4">
        <property type="entry name" value="NUMB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06311">
        <property type="entry name" value="NumbF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017607">
        <property type="entry name" value="Numb/numb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="5">
        <location>
            <begin position="37"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="328"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="485"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="498"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR017607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911929.1"/>
        </source>
    </evidence>
    <sequence length="542" mass="58204" checksum="F72F1A2F00BFB67B" modified="2020-10-07" version="1">MNKLRQSLRRKKPSYVPEASRPHQWQADEEAVRKGRCSFPVRYLGYVEVEESRGMQVCEEAVKKLKASGRKSVKSVLWVSADGLRVVDDKTKDLIVDQTIEKVSFCAPDRNFDKAFSYICRDGTTRRWICHGFLALKDSGERLSHAVGCAFAACLERKQKREKECGVTASFDASRTSFAREGSFRLPGPPAPTSRPSGARSPQDKKKVEAAVAPSAPIAAPAQPGSASPPQGSTSPEEKVEAGGAHAIPRRHAPLEQLVRQGSFRGFPTLSQNSPFKRQLSLRLNELPSTLQRKGHAESSAVSEGEAGASRDSDSISALCTQIDTSLAQPAGEPPAALVPAEGGPACSWDDPQASPLFQPGHKRTPSEAERWLEEVAKAAKAQQHQQVPQAAPPPLPAFPLSYEAAPLPTGIFASPHMPPTFVPVGTAYVPALPFPALPSVPVVGITPSQMVTNAFCSATQAPLTNLGVKASPFPQALLDPPRLRANGTVWPSEQSPPGIPAPHLEPPDPFEAQWAALESKASTAASNPFSGDRQKTFEIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAV2</accession>
    <name>A0A6I9XAV2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">OTU domain-containing protein 7B</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Cellular zinc finger anti-NF-kappa-B protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Zinc finger A20 domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Zinc finger protein Cezanne</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">OTUD7B</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 27">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C64 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013910932.1">
        <property type="nucleotide sequence ID" value="XM_014055457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAV2"/>
    <dbReference type="GeneID" id="106540370"/>
    <dbReference type="KEGG" id="tsr:106540370"/>
    <dbReference type="CTD" id="56957"/>
    <dbReference type="OrthoDB" id="10064699at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071947">
        <property type="term" value="P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035871">
        <property type="term" value="P:protein K11-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22772">
        <property type="entry name" value="OTU_OTUD7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14347">
        <property type="entry name" value="UBA_Cezanne_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000017">
        <property type="entry name" value="OTU domain-containing protein 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4770:FF:000003">
        <property type="entry name" value="OTU domain-containing protein 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054109">
        <property type="entry name" value="UBA_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF8">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22566">
        <property type="entry name" value="UBA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="23">
        <location>
            <begin position="184"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="24">
        <location>
            <begin position="837"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="446"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="457"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="554"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00077079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00081736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013910932.1"/>
        </source>
    </evidence>
    <sequence length="882" mass="97370" checksum="A9F6230CEF23E267" modified="2020-10-07" version="1">MTLDMDVVLSDFVRSTGAEPGLARDLLEGKNWDLNAALNDFEQLRQVHAGVLPRSFNDGRTFKFVEKEIARSVRPQLQRQDDIIQEKRLSRGISHASSTIVSLARSHVSSNGGSGGGGGGGGGGSEHLLEMPICTFQLPDLNAYSEDFRNFIERDLIEQSMLVALEQAGRLNWWANVDPSCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKSLYALMDRGAEREALKRRWRWQQTQQNKESGLVYTEEEWQKEWNELIKLASSEPRMHYGTSSGNCGGVEGSEEPVYESLEEFHVFVLSHVLKRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASKCHRSPLVLAYDQAHFSALVSMEQKEPIKEQAVIPLTDSEHKLLPVHFAVDPGKDWEWGKDDNDNVKLASVTLSLEAKLHLLHSYMNVKWIPLSCEMQAPLAQPESPTASAGDEIRSAADSGDSDKESVCSSSASNSGSSKASSKDKEKEKPKKEREKDKEKKRADSVANKLGSFGKTLGSKLKKNMGGLMHSRAVKGGITNGQGETLEKKKKGSIKIRKGSKEEASSGDFLGLPEKSGPAKTSSEKQPDNYKYSSDVKLSLSILRATMQGERKFIFASQLKTSHRHQYQEEMIQRYLLDAEERYSAEQKQKEAEKKMVALGNGGAAKKVDSEGHPLKGEETLLNSMDHPGLSPYSLQSSDLATGGTKMSSFPTGHSGVFTIPRPSPMSNVDSTYPPTYQDGRRQVAGGLCAPFPSYATLPRQCPQSRSHLSQQVGHFCAAEMDMPPSYAEVDCEGSSCPVLSSNGYQECTEPDGGSPKVVQGDRSKAWLLYSVQQTKCKQPNCSFYGHPETGNFCSCCYKEELRRKEQEKETLIHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XB83</accession>
    <name>A0A6I9XB83_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cancer-related nucleoside-triphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NTPCR</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912839.1">
        <property type="nucleotide sequence ID" value="XM_014057364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XB83"/>
    <dbReference type="GeneID" id="106541825"/>
    <dbReference type="KEGG" id="tsr:106541825"/>
    <dbReference type="CTD" id="84284"/>
    <dbReference type="OrthoDB" id="446244at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19482">
        <property type="entry name" value="RecA-like_Thep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00796">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004948">
        <property type="entry name" value="Nuc-triphosphatase_THEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010248">
        <property type="entry name" value="PRK13695.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146:SF1">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03266">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013912839.1"/>
        </source>
    </evidence>
    <sequence length="190" mass="20986" checksum="46B30BFA2C9FBB28" modified="2020-10-07" version="1">MATHVFLTGPPGIGKTTLIRKAAEILKSSGFPIDGFYTEEVRESGRRTGFDVVTLNGKRGILSRVGSGHSAARHEYRVGQYVVDLASFEQLALPLLRNTDFDSSSVKKVCIIDEIGKMELFSQTFIQAVQQILNGSGTVILGTIPVPKGKPLRLVEEIRNRKEVKVFSITRENRDNILQEVVTAVENCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBX4</accession>
    <name>A0A6I9XBX4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">T-box brain protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106540672</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911347.1">
        <property type="nucleotide sequence ID" value="XM_014055872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBX4"/>
    <dbReference type="GeneID" id="106540672"/>
    <dbReference type="KEGG" id="tsr:106540672"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021902">
        <property type="term" value="P:commitment of neuronal cell to specific neuron type in forebrain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000004">
        <property type="entry name" value="T-box, brain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF88">
        <property type="entry name" value="T-BOX BRAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="245"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013911347.1"/>
        </source>
    </evidence>
    <sequence length="448" mass="48045" checksum="0C3B7F5912AA1A04" modified="2020-10-07" version="1">MQLEHCLSPPSMMLSKKFLHVSAGGGGGGAYPPAAGGSELVLHEHLHPHPHHHPAAAAAAALLSASADNLERSSPLKKLARGGMTNQAEADNFPDSKDPPGDEQLRPTFDGAAAAAAAAAAAAAAAAAAAADRYLLSTPASSGQPPATASAPPAAAAATMFPYPGQHNHAAAFSLASPSRYMAHHPVIANGAYNSLLSNSAAPQGYPAAGYPYPQQYGHSYQGSPFYQFPSAQAGLVPGKAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNISGLDPTAHYNIFVDVILADPNHWRFQGGKWVPCGKADTNVQGNRVYMHPDSPNTGAHWMRQEISFGKLKLTNNKGASNNNGQMVVLQSLHKYQPRLHVVEVNEDGTEDTSQPGRVQTFTFPETQFIAVTAYQNTDTLNRQRANYKFVGLYYGSLLDIGKISLSSPRLCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDG8</accession>
    <name>A0A6I9XDG8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cyclin N-terminal domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CNTD2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911912.1">
        <property type="nucleotide sequence ID" value="XM_014056437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDG8"/>
    <dbReference type="GeneID" id="106541101"/>
    <dbReference type="KEGG" id="tsr:106541101"/>
    <dbReference type="CTD" id="79935"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd20537">
        <property type="entry name" value="CYCLIN_CCNO-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20542">
        <property type="entry name" value="CYCLIN_CNTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000057">
        <property type="entry name" value="Cyclin N-terminal domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027112175" evidence="4">
        <location>
            <begin position="25"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="148"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="6">
        <location>
            <begin position="241"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013911912.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="42909" checksum="2E32729B88CC922C" modified="2020-10-07" version="1">MAGPEGRPWTLLLLQRAVLSVGASFVGQTVSRVEEKREPLKSKSNEGRSTEKASGKKKGLESYEKEAEKRGCPQALPEAPLPRERVVPASLALAHELSQAMLRMDMALEREYAFDIFSNLMRKQPSYALQGFDLPRSVTVEMRALLVDWLVQVHEYLNLADETLHLAVHLMNCYMRAGRVRIPRLQLLSATCLFLACKLEEHTCPEPAQLCVMMENSCSRKDLLQMERKILARLRFELHYANPIHLFRLLAEVDHCTLEVQFLALYFLEASLMGVDCLRFEPAQLSFAALSLAHRLWREARIQDLDALQEYFPKFHLYSEAELLAVFPYMAKAVLQGANASLRAIFLKYSRPQKLCVSTSPTVAASKLLSRFLGDPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEG8</accession>
    <name>A0A6I9XEG8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reticulon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RTN1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibits amyloid precursor protein processing, probably by blocking BACE1 activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912586.1">
        <property type="nucleotide sequence ID" value="XM_014057111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEG8"/>
    <dbReference type="GeneID" id="106541608"/>
    <dbReference type="KEGG" id="tsr:106541608"/>
    <dbReference type="CTD" id="6252"/>
    <dbReference type="OrthoDB" id="567788at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
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        <property type="term" value="P:endoplasmic reticulum tubular network formation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="RTN1-4"/>
    </dbReference>
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        <property type="entry name" value="RETICULON-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="573"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="9">
        <location>
            <begin position="559"/>
            <end position="628"/>
        </location>
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            <begin position="386"/>
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            <begin position="424"/>
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            <begin position="525"/>
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        </location>
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            <begin position="37"/>
            <end position="49"/>
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            <begin position="58"/>
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            <begin position="248"/>
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            <begin position="281"/>
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            <begin position="314"/>
            <end position="325"/>
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            <begin position="454"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU210713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912586.1"/>
        </source>
    </evidence>
    <sequence length="628" mass="67676" checksum="4792437A1EEE7209" modified="2020-10-07" version="1">MSSPPPDLQSSEEHRAGDGAEDDSTAGASPRRWEGEGAQAPAAAFRAAGQPPPVAMATASTGGMTSSHFYDYGSSKTSDRSNGSLYTSLFSDELYTAPQDSSYFTGNLSREDSHVTPSESIEGIHAQSGLFSSDSGIEMTPAESSDVNKILADPAEQMKSESYKYMDISRSEEIKYQESCDTIKEDMSPLSLINKGVEDKIGAKGISFDEKHESKEGSSFLLDHQYSPSETSPVKITLTEIETVEVPLSKEKIPEKQEAGLKPSHEIVPTVTVSEPEDDSPGSITPPSSGTEPSGSESQGKGSLSEDELINAIKEAKGFPFKHPEGQGPQTVVPEKQDKKNKLTEFPAGSAPLDNEASSAESGDSEIELVSEDPLAAEEVLHSNYMTFSHAGGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREHDSPLMKPIIMDIIEEENHTRNEGTRASDYNLSGSKESRMNRENLADSASYLKCSFAASKVHADTVSSSTISSEEIKDKVAQKKSAEVAATASGKKSPSKNSVTRSPWSSPPLPFLNKQKAIDLLYWRDIKQTGIMFGSILLLLFSLTQFSVVSVIAYLALAALSATISFRIYKSVLQAVQKTDEGHPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEI6</accession>
    <name>A0A6I9XEI6_9SAUR</name>
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        <submittedName>
            <fullName evidence="5">Disks large homolog 5</fullName>
        </submittedName>
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        <name evidence="5" type="primary">DLG5</name>
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    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="disks large homolog 5 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="GK/Ca_channel_bsu"/>
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    </dbReference>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="GuKc"/>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
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    </dbReference>
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        <location>
            <begin position="35"/>
            <end position="125"/>
        </location>
    </feature>
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        </location>
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            <begin position="692"/>
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            <begin position="987"/>
            <end position="1061"/>
        </location>
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        </location>
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        <location>
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            <end position="651"/>
        </location>
    </feature>
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        <location>
            <begin position="779"/>
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        </location>
    </feature>
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        <location>
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            <end position="812"/>
        </location>
    </feature>
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        <location>
            <begin position="819"/>
            <end position="833"/>
        </location>
    </feature>
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        <location>
            <begin position="877"/>
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        </location>
    </feature>
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        <location>
            <begin position="888"/>
            <end position="898"/>
        </location>
    </feature>
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        <location>
            <begin position="899"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914059.1"/>
        </source>
    </evidence>
    <sequence length="1061" mass="116657" checksum="12E22B856B40F734" modified="2020-10-07" version="1">MENQLEKEARFRQLMAHSSHDSAIDTDSMEWETEVVEFEKDREDMDLKALGFDVAEGVNEPYLPGDCGIFVTKVDKGSIADGRLRVNDWLLKINDVDLTNKDKKQVIKAVLNGGGIINMLVRRRKSLGGKVVTPLHINLSGHKESGIGLENGIFVATLTSGSPAAKDGSLTVGDRIIAINGIALDNKSLTECEALLRNCRESLTLSLMKVYPQSSSSSWSGQNIFENLKDSEKISNGRVHASEVQVQNKRNLKHNSSTQTDIFNPDIMDVKKDQSDQENGLYCSLKPFSSSVLHSDSHRNTAHECHSNSEHSLESFGPDGYGDPSYGIVNLDNQRLSFDPTVADCIVLESALDKGPGGKHSGGTWPKVMVNVAPAETEKFSVYKKPKQRKSIFDPDTFKRPLTPPKMDYLSPNPMLGLSAQTSKPEAISTPPTPPTRSDSIKFKHKQQASSASESTVTMGSPPPSPAAVQPPLSIELKPDSCLLKGRGRPSGHYYREEGGDPGHLPSRKSCEEDVGFQRVEEPEIKRPRPKSAPAFRHKLTPVPIPNPSQQVDMGSCTQHQGHLSLDLSHKHTSECSENTRSRSSHGSNSLPSSARLGSSSNLQYRTERIKIPLTPRYPRSIIGSDRGSLSHSECSSPSLVTPPQSPLNLETSSFASSQSQNSLSTLPRISVSPVSMGQRRKDKPYMEEPRQVKVQKGAEPLGISIVCGENGGIFVSKVTSGSIAHQAGLEYGDQLLEFNGINLRNATEQQARLIIGQQCDTITILAQYNPHMFQLGNHSRSSSRLEPMSSHSTPQGSGAPTPDSHSIIDTVSEQDEGTMTPPSKDTTPTTSPRNSVRLGKAISRKRGEKDRGKKSAKMGQNSPFLCRMEQEFYRRHSMSEAKDDNSSSKASSAAARRSFFRRKHKHKRSGSKDGKDLLALDTISTDSIPFLDDAVSLAYQRVQKVDCTSPRPVLILGPLPDAVKDMLVKESPGKFCRCPLEVMKASQQAIERGVKDCLFIDYKRRSGHFDVTTVASIKEITEKDCHCLLDIAPHAIERLHSIHIYPIVIFVRYKNAKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFK0</accession>
    <name>A0A6I9XFK0_9SAUR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein KIAA0825-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106541907</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912946.1">
        <property type="nucleotide sequence ID" value="XM_014057471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFK0"/>
    <dbReference type="GeneID" id="106541907"/>
    <dbReference type="KEGG" id="tsr:106541907"/>
    <dbReference type="OrthoDB" id="10007406at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027993">
        <property type="entry name" value="DUF4495"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33960">
        <property type="entry name" value="SIMILAR TO KIAA0825 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33960:SF1">
        <property type="entry name" value="SIMILAR TO KIAA0825 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14906">
        <property type="entry name" value="DUF4495"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013912946.1"/>
        </source>
    </evidence>
    <sequence length="648" mass="74984" checksum="DCDC0D86454A6241" modified="2020-10-07" version="1">MWHYFCCALQRDLWTVLSPILAQEILTEVLEESLALLACRYFQAQPSYKRTPQIRTDVTAILMTCENMLWSVCGSMQELLDPHKDRNPRIYNIHRYCNNLLAAALVLTSPLENLFKKVQSIFDYLSSDILEQAVYQPLHWLAGISEFSSSLLKTPAGEMTTQCHLKLLLSQPYCNWNLLLRTLLHHDCRLVKILLRSFKSELSTSNEQSTCLDMKENNDINLAEAIFTVLSYCTLSPKSLGDALQNYMEKEHMWDFLHNISVSSCSELEPEVVKCLKSTLINSVNGTVKEIISLMHSWEASEKYGTYLPNKTVPESLLSTVPKEWNYIYKDVKTEDSRKSFTRLAAQVLSIVISNLPSVIACLPSPIKYFFLVSDKKIPEKFIESEKYGFLVKTLIIIICQIFEDGNIIELLTGAILDRWSKEKLSLVCICLETIIGKKKKNPKQAIHKIIQSIEQEKPNWIEKQLLKAKKLSTECTIITAKDSRSSEEETIELELTEQKINMMALDICHKPGGSEYLRQIYHIIQLNEDYLNKQLSYEGNSEEDPANIRHLRLILRSKEHSVFDPFQVHHWYCTNVLKKCAVNEWKWDWSDMLPSYLGQNRITFGTLLAHRWEMKEKETLKEEERAMLEEILKNYLTPPSLTSEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGU1</accession>
    <name>A0A6I9XGU1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable G-protein coupled receptor 83</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GPR83</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914919.1">
        <property type="nucleotide sequence ID" value="XM_014059444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGU1"/>
    <dbReference type="GeneID" id="106543430"/>
    <dbReference type="KEGG" id="tsr:106543430"/>
    <dbReference type="CTD" id="10888"/>
    <dbReference type="OrthoDB" id="5952899at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15389">
        <property type="entry name" value="7tmA_GPR83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238:SF69">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="81"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914919.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="47652" checksum="17E68E13491DC0C6" modified="2020-10-07" version="1">MNRHMWFSSPDVSKSLRWVGKSNSTNSTLFPFHNRSSFQEDLEVKSTGEFDTSYKEESQSQTVKALLIAAYLTIICISLLGNVVVCHVVLKNKRMPSATSLFIVNLAAVDILITLLNTPFTLVRFVSSTWVFGKPMCHISRFVQYCSIHMSALTFAAIALDRHQVIMHPFKPRMSLVKGGLCVLVIWVMAICFSLPYAIYQNLVRLSYGNGTIRMVCLSSFPPPTDLYWKYLDLATFVLMYLLPLSVISVTYISVAKKLWMRNAIGDVTVDQYYAHQRKKKMSLKMLMVVVIVFGICWFPLNCYLIFMSSQTVNHHNGFYFSFHWFAMSSACYNPFIYCWLNEGFRTEFRALLRMCCKKQTPSSQPLWSISADFRLSWDGNYCCKQEAATQRTRKSSHRNSAQTNISIIELITTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH19</accession>
    <name>A0A6I9XH19_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Nuclear factor of activated T-cells, cytoplasmic 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NFATC2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913406.1">
        <property type="nucleotide sequence ID" value="XM_014057931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH19"/>
    <dbReference type="GeneID" id="106542263"/>
    <dbReference type="KEGG" id="tsr:106542263"/>
    <dbReference type="CTD" id="4773"/>
    <dbReference type="OrthoDB" id="5346094at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01178">
        <property type="entry name" value="IPT_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF4">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="11">
        <location>
            <begin position="393"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013913406.1"/>
        </source>
    </evidence>
    <sequence length="924" mass="101811" checksum="658BCD2FA5C2E754" modified="2020-10-07" version="1">MASSPNDSDPNSQQGDDDPNPRISPQEELDFSILFDYDYSQPVAEEPTPSHKIISPPTGVTYPHDDVSDYGLKPYPFALSSLSADHMGRYGQPDSIGSKCYLDPHKAAGPPTLSPRIEITPSYELIHSGGAFSSRDTDLLVEHQSSSATASPRFTLPVPGFESYREPLCLSPPSSGSSASFISETNFSPYTSPCVSPNNGPSDDLCPQFQNTHAHYSPRTSPIMSPQASVVEDTCLGPRSPSPRPNSRSSSPGAKRRYSCTELYGSQHPHSSPRQSRTPSPQASPHVSLREDGATVTYTQLSTSSSLMDAMNNLTTDPSCGVPTKIWRTTPDPSPLVSHKASMPCHVLQPIEYIGSCEQENRRNPPPDSILLVPPSWPKQLMPAIPICSIPVQSLPPLEWPLSSHSGAYELQIEVQPKPHHRAHYETEGSRGAVKAQTGGHPVIQLHGYVDHKPLGLQIFIGTADERILKPHAFYQVHRITGKTVTTTSFEKIIGNTKVLEIPLEPKNNMKATIDCAGILKLRNADIELRKGETDIGRKNTRVRLVFRVHIPESSGRIVSLQVASNPIECSQRSAHELPMVERQDVDTCLVYGGQQMILTGQNFTAESKVIFTEKTPDGQQVWEMEATVDKDKSQSNMLFVEIPEYRNKHIRVPVKVNFYVINGKRKKSQPQHFIYHPVPSIKTEPIDDYDPSLICNTVHSGLRTVTQPYYSHHTMASESPSCLVATMASCQQLHSNLSSPESQYPSQNPGAIIYQRSKSLSPSHLGYQQSNLMGAQVPIPDVHRSVLVHTGSPSQSSAMLHHSPANQPPSPVIHCSPTANHQMRCESHQEFQHIMCSENFTSSGVRPHQPQVNQAQRLSPSSYPTVIQQQRTAKNGPPVNDQKEVVSAGVTVKQEQNLDQAYLDDVNEIIRKEFSGLPARSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI14</accession>
    <name>A0A6I9XI14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear transcriptional regulator 1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NUPR1L</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NUPR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915384.1">
        <property type="nucleotide sequence ID" value="XM_014059909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI14"/>
    <dbReference type="GeneID" id="106543822"/>
    <dbReference type="KEGG" id="tsr:106543822"/>
    <dbReference type="CTD" id="389493"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018792">
        <property type="entry name" value="NUPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149">
        <property type="entry name" value="NUCLEAR PROTEIN 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149:SF3">
        <property type="entry name" value="NUCLEAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10195">
        <property type="entry name" value="Phospho_p8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915384.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="13211" checksum="7C15F1C42C589C8F" modified="2020-10-07" version="1">MRSGRSGTRASVVAALSSGSEDSEWEEERVARLRERAWRDGPRGPGSGGKGRSRRSREQRTNWRVPAGHERKITAKIRASLAKRRLQQRPGRGPRSAPLQPRKRAEAKNAKTPEMVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI62</accession>
    <name>A0A6I9XI62_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase zeta catalytic subunit</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein reversionless 3-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">REV3L</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translesion DNA synthesis (TLS). Lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="18">Heterodimer with MAD2L2. This dimer forms the minimal DNA polymerase zeta complex (Pol-zeta2), with REV3L bearing DNA polymerase catalytic activity, although its activity is very low in this context. Component of the tetrameric Pol-zeta complex (Pol-zeta4), which consists of REV3L, MAD2L2, POLD2 and POLD3; Pol-zeta4 is the fully active form of DNA polymerase zeta.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013910613.1">
        <property type="nucleotide sequence ID" value="XM_014055138.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540116"/>
    <dbReference type="KEGG" id="tsr:106540116"/>
    <dbReference type="CTD" id="5980"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22287">
        <property type="entry name" value="REV3L_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000024">
        <property type="entry name" value="DNA polymerase zeta catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000002">
        <property type="entry name" value="DNA polymerase zeta catalytic subunit isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000005">
        <property type="entry name" value="Putative DNA polymerase zeta catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032757">
        <property type="entry name" value="DUF4683"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15735">
        <property type="entry name" value="DUF4683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="26">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="25">
        <location>
            <begin position="56"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4683" evidence="24">
        <location>
            <begin position="720"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="22">
        <location>
            <begin position="2193"/>
            <end position="2445"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="21">
        <location>
            <begin position="2511"/>
            <end position="2961"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="23">
        <location>
            <begin position="3003"/>
            <end position="3070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="260"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="460"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="511"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1011"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1157"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1534"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1809"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1928"/>
            <end position="1947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2075"/>
            <end position="2112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="485"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="521"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1017"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1173"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1555"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1814"/>
            <end position="1830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="2100"/>
            <end position="2110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF15735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013910613.1"/>
        </source>
    </evidence>
    <sequence length="3091" mass="349341" checksum="5CE78EAEE2482A5B" modified="2020-10-07" version="1">MFSVRVVTADYYMSSPLPGLDPCQSSFREAPVKKVPVVRVFGATPSGQKTCLHLHGIFPYLYVPYDGYGHLPDNYLRSLAFSIDRALNVTLGNPSSSVQHVFKVSLVYGMPFYGYHEKERQFMKIYLYNPAMVKRVCELLQGGAIMNKIYQPHEAHIPYLLQLFIDYNLYGMNLINLAAVKFRKIKKKGDAFEEIASCGDNWTKSATRPHQWEDNQIPSSLILEGIEPQSTCELEVDAVAADILNRLDIEAQIGQNPGLQAIWEDEKQRRRQNKESSQITPPNSQDRGFVAPTESERIFQKRLKEILKQSDFSVTSGSIDSNNESQEFSAELTLHSEIPKIVSCSPANMIEVHSDKELHREDSESKYKEDALINEEAILNIMENSQYFQPVSQRFMNNSADQAMIDLLAELEDDGYEIVQQNASSQHYSSGHHRNLQNSDDEENEPQIEKEEMELSLLMSQQWDSSSNDDAKRRSMGRSRNQSSGEDDSSSEDDMDWNDNVLLASLSIPQLDGTADENGDNPLNNEGSRTHTSAIATSKTVKTTIFHKDAATLEPPSSAKITFQCKHTSALSSHVLNTDDLEGLTQPSMIDIRPDLSIHSLSNESIYNMKYPASFNNILHSEKPHQENNKSEKGPVLCEDISSVNRHLPSRKYAGIRKVAKDVSQHMNHQISENILTKKSNFVDLVYSKNKTSSEINIKANTTDLNSLFPAPENCETMSCSETQTLILSSEKSTDGDSLNNFKLRYEEFEEHKTEKTNVNQQAAHYMFFPSVVLSNCLNQPQKLGPVTYKLQHCKKQSKLKRSKKKMVLSNQQSSSAMDTLSTKESCYVQDNASSNISDRDILLPNDTQIHHTVFENKMSKDTRNYTDCHFEDFAQESEQTFGLCSNKYTLRAKRKINYETEDSESSLGIHNSKIMLPHSTVENDEDVDGSKKFRKRRRLSRKLPPVIIKYIIINRFRGRKNMLVKLGKIDSSEEQVLLTEERMNLYKKLAPLKDFWPKVPDSPATKYPIYPLTPKKSNKRKAKCKSTKKRLGGNPQNIKSKTIRRTLYFRKRTHAFLSAPLPSYNAETEDCDLNYSDVMSKLGFLSERSASPVNVSPPRCWSPTDPNAEEIACNLEKETLVFKNTNTFSSKTVNSNVGKHVQSKTQVKKCRKQFANATIRSKKSNQSNKPRNNLKKKPRSKQKQKFKKVSTLVTVADGQNSIPPVTERKPTPHPWDLPENVHNLGNSQPFTKELASTSCTTGHPSSAQLSLTANAQVNPLIYFQSLLENEKPIGHQGFSLTEDLHPDIASLISGREKAKINFQRSNSQSGIFRMEESSVQNNVYDQCNCISQVALKAYSSKDALKKTEEVTNSQNKLLSSVGKVNENCISSDVINTDQIHPGNLLHCKDNQQQIVHLSEATKQADHISSFLKTSGESHGSFQLPKKCFIASLRSPMNKLEWEQKQKGFILDVAHFSPEKNTKQKVVSETISQAQTDSQCKSKGLVTPSAFNEGQSGLAVLKELLQKRQQKMHMNNIHEQASVKSQSNIDASLFADSNKPAKKPGSVASSRKPRIPRDKKSKEKVVKLLKDSAKQLSNKTGHVTLTDTSILYSDAGFESCYSLEDSLSPDHNYNFDINAIGQTGFCSLYSASQFVPADQNLPQKFLSDVAQDHYPGQTAETEFLNHNKVSKEENMHNTDTQSWLRSGQHSPQRFDKRCLENNENLLHNQWKTILPSTSQPGSPAVGQQAEMCHKEQLQLDRNIMKKAIFLNLSSPNGDWSQNPFRKEVISESGQLLDSAGISFSSILSSPDGELMDAASEDLELYISRNNEGLTPTPDSSPRSTSSPSQSKNGSSTVRAAHILKPLMSPPSREEIMSTLLDHDLAETIYQEPFCSNPSDVPEKPREIGGRVLTVESRLPNDLLEFEGDFSLEGLQLWKTAFLAMAQSPRPGSPLRNSQFSTDKKQRNTRKINDKKIVIMPCKCAPSYQQIQSWLQAKEDYEYFKKRAVIKSIEIEKIDESLNNMSLPKNLCDQIVKVAVSSGTPSKPAVQSEDLCFSSSNPTSPQTMEKFGVNVSEMSKSTVKVKTMPLVPAAIDKDKKEEDCTNYSSPDSPGLPPWQQVVPTDSKQSNLEDSEGILSSLEENKSVAEKEQKSVKKESKIPFCVSPLSKEPDGNSDICFHSTPIREHMSKEEALGCTPLSAIGRDIQKFSSRRGRNVDALRKVLLTTQIKNQFTTINTQKKETSQIEGPSLNNSYGFKISVQNLQEAKALHEVQYLTLLSMELHARTRRDLEADPEFDPICALFYCISSDTPLPDTEKMEITGAIVIDKDRARTISSQGNREHAPLLARSGISGLEVTYATDERALFQEVVNIVKRYDPDIMLGYEVQMHSWGYLLQRAAALDVDLCQMISRVPDDKKENRFASDLDEYGADTMSEINIVGRVILNIWRIMRHEVALTNYTFENVSFHILHQRFPLFTSRVLSDWFDNKADIYRWKIVDYYISRVRGNIQLLEQVDLIGRTSEMARLFGIQFLHVLTRGSQYRVESMMLRIAKPMNYIAVTPGVQQRAQMRAPQCIPLIMEPESRFYSNTVLVLDFQSLYPSIVIAYNYCFSTCLGHIENLGKNDEFKFGCTSLKVSPDLLYQLRHDITISPSGIAFVKPSVRKGVLPKMLEEILKTRIMVKQSMKAYKLDKTITRMLDARQLGLKLIANVTFGYTAANFSGRMPCSEIADSIVHKARETLERAIKLVNDTKKWGARVVYGDTDSMFVLLKGATKEQSFKIGQEIAEAVTATNPKPVKLKFEKVYLPCILQTKKRYVGYMYETLDQKDPVFDAKGIETVRRDACPAVSKILERSIKLLFETRDISHIKQYVQNQCMKLLEGKASMQDFIFAKEYRGSSAYRPGACVPALEITRKMLAYDRRSEPRVGERVPYVIVYGMPGLPLIQLVRRPIDVLQDPNLRLNATYYITKQILPPLARILSLIGIDVFGWYNELPRIQKVSTISRTEQECRKGTISQYFTTLHCPVCDELTQHGICNKCRSQPQHVIVMLNQEIRELERKHEQITKICKNCTSCFDRQIPCVSLNCPVLFKVSRVSRELSKAPYLRQLLDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIA6</accession>
    <name>A0A6I9XIA6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ras GTPase-activating protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106542536</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913791.1">
        <property type="nucleotide sequence ID" value="XM_014058316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIA6"/>
    <dbReference type="GeneID" id="106542536"/>
    <dbReference type="KEGG" id="tsr:106542536"/>
    <dbReference type="OrthoDB" id="1562946at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000283">
        <property type="entry name" value="Ras GTPase-activating protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001562">
        <property type="entry name" value="Znf_Btk_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF4">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00779">
        <property type="entry name" value="BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51113">
        <property type="entry name" value="ZF_BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="22"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="9">
        <location>
            <begin position="209"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="473"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913791.1"/>
        </source>
    </evidence>
    <sequence length="643" mass="73503" checksum="2BA46A20B0638588" modified="2020-10-07" version="1">MLLFLGYSGWLNLTEIDPDEEVQGEIHLQIEIIGNGAARKLRCVVFDARDLARKDRNGASDPFVRVRYNSKIQETSVIKKSCYPKWNETFEFDLDESAAENLCIVEVWDWDLVSRNDFLGKVVFNVQRLKDVQREEGWFLLRPDKSKPRLDEGNLGSLHLQVQLRDEMVLPSIYYQPLVELLCQEVKAGIKNQKVHLITLIDETTTAECRQELAVNLVKLFLGQGLIKEFLDLLFKLELDRTSEPNTLFRSNSLASKSMESFLKVAGMHYLHRILRPSINRVFEEKRYIELDPSKVESKEIGCSSLHRIHSESEVIQQSGQLLQSYLTDLLNTITRSAKICPPVIRATFQLLFKRVGERFSEEKNQNVKFIAITSFLCLRFFSPAIMSPKLFHLREKHADAHISRALLLLAKAVQNVGNMDNSISRTKEAWMAPLQAIVQRGVAQMKQFILQLIDIEEKDELDLQKPISLQPQVVKEGYLFIHKARSKGPLLSFSFKKLYFSLTHEALSCAKTPNSKKSSFVPLTSIRAAEKVEEKSFGISHVMQIIYTNDAGQEDTAYLQCKCVNELSQWLSALRKVCGNNAGMLCSYHPGVFRGDKWSCCHLKNKAGLGCDKTRHGVTLREWNDPLDPDLEAQLIFQHLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIH5</accession>
    <name>A0A6I9XIH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106540199</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910718.1">
        <property type="nucleotide sequence ID" value="XM_014055243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIH5"/>
    <dbReference type="GeneID" id="106540199"/>
    <dbReference type="KEGG" id="tsr:106540199"/>
    <dbReference type="OrthoDB" id="6608471at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF14">
        <property type="entry name" value="SIDEROFLEXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013910718.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="35402" checksum="C3280BE12AD14E67" modified="2020-10-07" version="1">MASITKFNIDAPRWDQNTFLGRVKHFFNITDPRTLLVSEQTLDSAKTIVDNCRAGSVPPGTSEEQLFYAKKLYDSAFHPDSGEKMNLIGRMSFQVPGGMAITGCMLQFYRTVPAVVFWQWVNQSFNAIVNYTNRNAASPISVKQIGVAYFTATSTALATAVGLNLYTKRAPSLVARWVPFAAVAAANCVNIPLMRQQELINGITVTDENDNTLGKSRRAAIKGITQVVVSRIVMAAPGMILLPIIMEALEKYPFMKKIQVLHAPLQTVLVGGFLVFMVPVACALFPQRSSIALSHLEPELRERIVAEHGDKVTFAYFNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJH5</accession>
    <name>A0A6I9XJH5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Talin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TLN2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914181.1">
        <property type="nucleotide sequence ID" value="XM_014058706.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542862"/>
    <dbReference type="KEGG" id="tsr:106542862"/>
    <dbReference type="CTD" id="83660"/>
    <dbReference type="OrthoDB" id="10262320at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10569">
        <property type="entry name" value="FERM_C_Talin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000005">
        <property type="entry name" value="Talin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000066">
        <property type="entry name" value="Talin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000003">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000004">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000001">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000004">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000007">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000007">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000028">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057346">
        <property type="entry name" value="Talin1/2_VBS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015224">
        <property type="entry name" value="Talin_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036476">
        <property type="entry name" value="Talin_cent_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054082">
        <property type="entry name" value="Talin_IBS2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049108">
        <property type="entry name" value="Talin_R4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981">
        <property type="entry name" value="TALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981:SF34">
        <property type="entry name" value="TALIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21896">
        <property type="entry name" value="Talin_IBS2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09141">
        <property type="entry name" value="Talin_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21692">
        <property type="entry name" value="Talin_R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25177">
        <property type="entry name" value="Talin_VBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109880">
        <property type="entry name" value="A middle domain of Talin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="5">
        <location>
            <begin position="15"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="6">
        <location>
            <begin position="484"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="6">
        <location>
            <begin position="1147"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1354"/>
            <end position="1381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013914181.1"/>
        </source>
    </evidence>
    <sequence length="1395" mass="150977" checksum="2F6032AC89223ED0" modified="2020-10-07" version="1">MLRNGDVLEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQEGIEEKKEEGTGTLKKDRTLLRDEKKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGAFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESISPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSTGPETFNMGSMPSPQQQVTVGQMHRAHMPPLTSAQQALMGTITTSMQAVQQTQADLTHVDDLPPLGNDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPVDTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLKAARTLAGAVSNLLKAVEPTSGEPRQTVLTAAGSIGQASGELLRQIGENEADERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALNDLLQHVRQFASRGEPIGRYDQATDTIMYVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPDNEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAISNKNSAAHQQLVQSCKNVADHIPQLVQGVRGSQAQAEDLGAQLTLINSSQSFLQPGSKMVASAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALNTVQTLKNELQDAKMAALDGQLKPLPGETVFQSRDVPEKTCSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVADMLAACKQASYHPDVSEEVRLRALHYGTECTLAYLQLLEHVLMILQKPSAELKHPLTALSKGVAGAVTELIQSAEAMKGTEWVNPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQVAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPSELREEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKF6</accession>
    <name>A0A6I9XKF6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Myomesin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MYOM1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916279.1">
        <property type="nucleotide sequence ID" value="XM_014060804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKF6"/>
    <dbReference type="GeneID" id="106544508"/>
    <dbReference type="KEGG" id="tsr:106544508"/>
    <dbReference type="CTD" id="8736"/>
    <dbReference type="OrthoDB" id="8776562at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20951">
        <property type="entry name" value="IgI_titin_I1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000069">
        <property type="entry name" value="Alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000124">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000134">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000192">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000233">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000467">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000179">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF16">
        <property type="entry name" value="MYOMESIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="263"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="385"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="501"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="775"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="1001"/>
            <end position="1079"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013916279.1"/>
        </source>
    </evidence>
    <sequence length="1103" mass="125194" checksum="92D39B9471EE982F" modified="2020-10-07" version="1">MSLPFYQRHHQHFDRAYRHKELESTLSQYQKEEKRKSAIYTHGSTAYSHRGASAYRQESAFSDQKSTEASQELIEAHAHKSAAHSKRSAAYTLGSELVNKKAEAYHHGSELINKKAEAYRLGLGYDAYSLGSLAEDHTLKLSPKAKRAKQSVLSDEKENTIIEYVVPVFSGRENYISGITDKEEERIKESAAYIARRNLFAAGEGITTTKKKISSSVEEEHHEKKSRKVAIRETAERLALKKTITETQEFHRKLNEDSLLHAPEFVIKPRSHTVWEKQNARLHCTVRGWPEPRVTWYKNNVPINIHTHPGKYEIQSRYGMHSLEINKCDFDDTAQYRASAMNVKGELSAYASVVVKRYKKGEFDESFFHAGTVTLPLSFGLGVSPYGYASRFEIHFVDAFDVSFGREGETMSLGCTVVIHPDIKRFHPEIQWYRNGVLISPSKWVQMHWSGERASLTLTHANKEDEGLYTLRVIMGDYYEEYSAYVFVGDADADIAGVPGSPLDVKCLDANKDYVIISWKQPAVDGGHPILGYFIDKCEVGTTHWSQCNETPVKFARFPVTGLIEGRSYIFRVRAVNNVGISIPSRISESVAALDPADRARLKSHPSAPWTGQIIVTEEEPSGNTFFCTEINILFLDHICIEAYSPFCLGFSHETLYMDFVPLKSELAVEILEKGQVRFWLQAEKLSSNAKVNFVFNDKEIFNGEKYKMKIDHNSGMIEMFMDKLDDADEGTYTFQLQDGKATNQSSLVLIGDVFQKLQKEADFQRQEWFRKQGPHFVEYLGWEVTPECNVLLKCKVANIKKETHVIWYKDDREIMVDEKHDFQDGVCTLLITEFSKKDAGIYEVILKDERGKDKSILKLVEGAFTDLMTEVCRKIALSATDLKIQSTAEGIRLYSFVNYYLEDLRVGWSHNEVKIKYTERVKTGVTGNQIWLQINEPTTNDKGKYILELFDGKTTHTKMVDLSGQAFDDAFAEFQRLKTAAIAEKNRARVLGGLPDVVTIQEGKALNLTCSVWGNPVPEISWLKNERLLKADDNIILKYESGKQACFTITAVSTIDSGKYSLVVKNQYGTETSDFTVSVFIPEEGVEIPHPELGKGDKKKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKT2</accession>
    <name>A0A6I9XKT2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 260-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106543167</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914562.1">
        <property type="nucleotide sequence ID" value="XM_014059087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKT2"/>
    <dbReference type="GeneID" id="106543167"/>
    <dbReference type="KEGG" id="tsr:106543167"/>
    <dbReference type="OrthoDB" id="9025406at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000739">
        <property type="entry name" value="Zgc:171418 protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000566">
        <property type="entry name" value="zinc finger protein 133 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000681">
        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001009">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000620">
        <property type="entry name" value="Zinc finger protein 263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000204">
        <property type="entry name" value="Zinc finger protein 331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000342">
        <property type="entry name" value="zinc finger protein 394"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001882">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000038">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000290">
        <property type="entry name" value="Zinc finger protein 697 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000099">
        <property type="entry name" value="Zinc finger protein 79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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            <dbReference type="RefSeq" id="XP_013914562.1"/>
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    <sequence length="701" mass="82435" checksum="3CE7276A65367798" modified="2020-10-07" version="1">METMQEGKCVNRRNQERLFECSECGKSFAYRSLLLIHRKLHTGNGSHLCFLCGKSFPGVWSFAKHLRSHPGLKPYKCGECGERFANGSDLGTHQRIHQGKRSQESWKHWGRFPERRHQCMQCGLCFSQTSQLLKHQQIHTAGKPYQCLKCGKSFRYKQLFRRHEKIHTVQSPYKCLECGKCFTQNSSLCQHNKVHTKDESKTCLECGKSFSSKSCLLKHQRIHTGEKSHKCQECGRCFGRKSNLIRHQRLHTGDRPYKCPKCEKRFVESAELRRHQRTHWGKKLYKCKSHLIRHQRIHTGEIPHQCPKCGRCFGYKSCLIRHQRLHTGERPHQCPECGRCFGRKSHLIRHHKLHTEEKPYQCPECEKRFVNSSRLQRHQKTHRREKLYKCKDCDKCFLKMSYLSQHQSIHTGVKPYMCIECGTFFRTIQILRNHANVHRGEKLFKCLECGKAFGRSSSLRIHCQTHTGEKCHKCSVCEKSFTRKDTLWMHQRSHLEKQYKCPECEKSFRCKYYLRKHERIHKGEKPSKPVQKNEKCSECGRCFNTKSQLNQHQRVHTGEKPHQCPECGRCFASKSCLRKHQRIHTGERPHQCPDCGRCFVQKGHLIRHQKLHTGDRPYQCSECEKGFVDSAGLQRHQKKHTGERPYKCGECGKGFLRATQLQVHQRTHTGEKPYPCMECGKGFSRKEHLGRHQKVHSRVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM84</accession>
    <name>A0A6I9XM84_9SAUR</name>
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        <submittedName>
            <fullName evidence="4">U7 snRNA-associated Sm-like protein LSm11</fullName>
        </submittedName>
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        <name evidence="4" type="primary">LSM11</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
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            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Lsm11"/>
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        <property type="entry name" value="Lsm11_M"/>
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        <property type="entry name" value="LSM_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
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    <dbReference type="PANTHER" id="PTHR21415">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915041.1"/>
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    <sequence length="353" mass="40145" checksum="B9BE6D6C84E5ABE9" modified="2020-10-07" version="1">MEEEAAEKAGAEPRRRSRRHSSGRRSSERSPSPGRLDVSSPRFDPLLALYSERTPLPYPAAPCFNNLAEYESFQRGLLRAPGRSRTAAGRSSRSAGTRTRSRAGRPPADPERLQRLRNLMLVKEPKPEAEERARSRGRKAPRNVLTKMSLHEGSPLGELHRCVRDGVKVNVHIRTFKGLRGVCTGFLVAFDKFWNMALSDVDETYRKPVLGKAFYVEPQLTLTRLFDRLRLQESLVKKEADSKTTTDFPALPYDPRTSRRKSESGRGRSEEKHGAKKHASRERTRSSSLPKVSGRDMELPSRLSQVEEKSTGGKRGRSRKKQRSRVDYQQVFTRHINQIFIRGENVLLVHLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMB1</accession>
    <name>A0A6I9XMB1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calmodulin-regulated spectrin-associated protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CAMSAP3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915066.1">
        <property type="nucleotide sequence ID" value="XM_014059591.1"/>
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    <dbReference type="GeneID" id="106543543"/>
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    <dbReference type="OrthoDB" id="2125658at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000001">
        <property type="entry name" value="Calmodulin-regulated spectrin-associated protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
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        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF2">
        <property type="entry name" value="CALMODULIN-REGULATED SPECTRIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="237"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="9">
        <location>
            <begin position="1199"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
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        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="571"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="739"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="800"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="924"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1021"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1158"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="374"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="647"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="667"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="851"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="973"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013915066.1"/>
        </source>
    </evidence>
    <sequence length="1340" mass="146905" checksum="BF810431090E5033" modified="2020-10-07" version="1">MVAAAPAAAAAMRKSFLVPEIKPLDQYDFPRARSAASLAWVLAKAYGGAEHIPAELREPFYTDQYEQEHMKPPLIRLLLSSDVYCRAWCQVLPSGPDGTLPPKDNAALLQLLAHRGLVPTFQDKPVKEADLHHKPIKLGAHLALIDSLMTAFALEATSSLSVALGIEPATVSWDQKLLRWIDMIIHKIQQTAEQESSQRSAPGTDGQTPASCSGPKCPTRWYWKLVPHAIAFCLKESGNKPPVIRYRKDKALPKQTPCFPAITGIKDLASGGAIAATIHYYCPQLIRLEDVCLKETMSVADSLYNLQLIQGFSSDYLGGSCFLSLEDLLYMPPLLRVNVGVFLAELFLCFEVVKPDFVCPKELPRVQDSPGLNDASTPNSGKNSSSPVFSLRHPLLPGGQAQSPLCGSLGSLHHSTSMSQVEGFGKSWSKKQLSHPLSQAVSFSIPFGLDSDVDIVMGNPVGILRSVSSDSLAPTLYSSLPRSSHPWPTEAAEAPSGLVMPSLHGAGHKNQEGPALENGLNDGYPDLPTIEEALQIIHSSERLVPEGAPDGFYLHSPEPPRTSVLEKTPLPTVYRCHNGGPNGAEPVQEGSLDSDAEEPLRLAGGPDDSTSGVSSLSSQPESTASSVSGVRMTSFAERKKKLAATDSKSSGISSEGSEAGQGPSEESPAQSPALSSEMSQLSSRLEEKRRAIEAQKKRIEAIFTRHRQRLGKSAFLQLQKAPDSQSEDGRLALEERLAQLEPEEEAGSPRTRLDNKQVTFSPDITKGGPLDENLGDYNRAVAKLNTALSSLQLDMQRLSEQQQRLMQEKKPSAQAWVIPAPRTGREGTPPRSSLELSSPSPSPSRKSRSPQPTPKKSPAPMPPKSPKHTRPVELKLPPLTRVLTPPHNVDNLPHLRKFSPSQVPVQTRSSIHFAEEVLPVPEVEAQNNLRPVASVPPQGGNARVSGDGTSDVSSPSDRHSSLIEIPLSSLKGEEDEDVDGDDSLEESIAETLDSEPRTGLGFFFKDEDKAEEEMAQKRATFLERQQRRSEEARRRKQWQEAEKEQKKEEAKIQTDERARVEEEVGPRRGDFTRQEYQRRHQLKLMDDLDKVLRQKPTTVRALKKGRPKTVFCDDSALARSPIKGLLGSKLSKVYSQSTLSLSTVANDPGNSLFVKRASRAASPSGPMSPNRLLPNQNRERDWENASTASSPASIPEYTGPKLYKEPSAKSNKYIIHNALSHCCLAGKVNEPQKIRILEEVEKSKANHFLILFRDSSCQFRALYTLSGETDELTRLAGYGPRTITLPMIEGIYKYSSDRKRFTQIPSKTMSMSVDAFTIQGHLWQTKKPGTPKKPSTPKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMD3</accession>
    <name>A0A6I9XMD3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent RNA helicase TDRD9</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Tudor domain-containing protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TDRD9</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013915067.1">
        <property type="nucleotide sequence ID" value="XM_014059592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMD3"/>
    <dbReference type="GeneID" id="106543547"/>
    <dbReference type="KEGG" id="tsr:106543547"/>
    <dbReference type="CTD" id="122402"/>
    <dbReference type="OrthoDB" id="66977at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001113">
        <property type="entry name" value="ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000946">
        <property type="entry name" value="putative ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000016">
        <property type="entry name" value="Tudor domain containing 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF113">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10 22" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="12" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="120"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="350"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013915067.1"/>
        </source>
    </evidence>
    <sequence length="1191" mass="134397" checksum="7835A07CE34FC4F6" modified="2020-10-07" version="1">MLRKLTVDQINDWFTIGKAVSGVEFLGGEVRTAGFAHHLVESVEEPPPAANVTRVLQNDGEEYIKRYRQLEVKELEAEGGRCSSYKPGSPQHESTFMSYIPDGDYKYPDLPISRYKEEMLSLIENHSVVIVHGSTGSGKSTQLPQYVLDHCAQHSIYCNIAVTQPRRIGASSIARWISKQRSWPLGELVGYQVGLEKVATKNTKLIYMTTGVLLQKVVGAKSLLEFTHIFIDEIHERTEDMDLLLLVIRKLLRTNSRFVKVILMSATIDCQEFANYFALPVRNKLYPAYVFQVEGKPHVIDKYYLDDLKPVLSHIKIPLQIIEEPLIHPVMYKAAIALIQWFDELETKENGDRNLEFVSERGSVLVFLPGLAEITYLLELLTNMVHRRLQVYPLHSSVTLEEQNNVFLAPVPGYRKILLSTNIAQSSVTVPDVKYVIDFCLSKNLVCDEDTSYQSLRLCWASKNSCDQRKGRAGRVSKGYCYRLVYKDFWANCIPESTVPEMMRCPLETTVLKVKMLDMGEPRALLATALSPPVSSDIERTILHLKEVGALAINDHFEERNPYDGELTFLGKVLAQLPVNHHLGKLIVLGHVFGCLEETLIIAAALSLNSFFVIPFKQHLDGYSQNEVDWGRSNYIHIKRMREMAELYEDLKKRVGVFNMHVNTHSPIMDQEHAYKQRFILQVVIAGAFYPNYFTFVHHDKEVAVRELSGRDPKTTVMLKNIPHYGFLYYKQLQSLFRQCGQVKSIVYDGTKSLYVDCGSVMVFYVDYGNRSKVALDQLREIPGDLRELPFQALEFKVRRIRPSAWSLVCGEQWSYAANQRFASLVSGHVLTVKIFSIVHGILHVDVFQSSGIANMVNICTILIEEGYAEPADDSYESKLSLVQLSLTVFLMCEKEFLTCHEEGVDESRTRYTGVLCGLGWNQSLGSPVLQDHDMELAFDVQIDADDITQVTLLGPFNAYNLKFSSMTRISRFRTILVEKESVNSVVVVDAPENAFQQILVAASISVNATGSVMMLKETSLMPHIPGLPALLSMLFAPVIDLRVDESRTRYTGVLCGLGWNQSLGSPVLQDHDMELAFDVQIDADDITQLVFELLGYGSKEREVPITLAQGSPTPGVQTATSLQPVGNRGTEAVLICKSKPREAVPPTWYKKSYEWTQVRSSLIIDQSDQPAVSRKNSSLFQLHKLVLLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XME9</accession>
    <name>A0A6I9XME9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dysferlin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106543578</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915101.1">
        <property type="nucleotide sequence ID" value="XM_014059626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XME9"/>
    <dbReference type="GeneID" id="106543578"/>
    <dbReference type="KEGG" id="tsr:106543578"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002281">
        <property type="term" value="P:macrophage activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002280">
        <property type="term" value="P:monocyte activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050765">
        <property type="term" value="P:negative regulation of phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001778">
        <property type="term" value="P:plasma membrane repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033292">
        <property type="term" value="P:T-tubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08373">
        <property type="entry name" value="C2A_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04011">
        <property type="entry name" value="C2B_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04018">
        <property type="entry name" value="C2C_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000033">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000037">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000061">
        <property type="entry name" value="dysferlin isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037726">
        <property type="entry name" value="C2A_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037720">
        <property type="entry name" value="C2B_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037722">
        <property type="entry name" value="C2C_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012968">
        <property type="entry name" value="FerIin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012560">
        <property type="entry name" value="Ferlin_A-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012561">
        <property type="entry name" value="Ferlin_B-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF44">
        <property type="entry name" value="DYSFERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08165">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08150">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08151">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01200">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01201">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01202">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="194"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="354"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013915101.1"/>
        </source>
    </evidence>
    <sequence length="865" mass="96743" checksum="6D494361F121F721" modified="2020-10-07" version="1">MLRVFILHAQQVQTPDTDISDAYCSVVFSGVKKRTKVIKNSTNPVWNEGFEWDLKGVPLDQNAELLVVVKDHETMGRNRFLGEAQVPLRDVLASPNLAASFNAPLLDAKRQNTGGCLMLQVSYTPPPGAAPFIPPPAPPEPNPTLPELDTVTDTGGEEDSEDQLGTEEEAEPSARPSAPDQPSLPKRPPSHVLPASRRRKSTSRKLLSNKPQDFQIRVRVIEGRQLSGVNLKSVVKVTAVGQTKRTRIRKGNGPFFDETFFFNVFESPVELFDQPIFLTVVDSRSLRTDSVIGEFRVDVGTIYAEPRHCFLRKWLLLSDPEDFSVGARGYLKVSLYVLGPGDEAPVEKKEAAEEKEDIEGNVFKPPGVALRGAHFSLKVYRAEDLPQMDDAVMDSVKQIFGFESQKKNLVDPFVEVTFAGKTLCSKIVEKNANPQWNQIVTLPAMFPSMCERMRIRVMDWDRLTHNDIIGTSYLSMSKISAPGGELEVDDGLGFLPTFGPCYINLYGSPREFTGFPDPYEDLNSGKGEGVAYRGRLLVELETKLVDYVEQKLGDIPADDILRVEKFLRRRKYNLFAAFYSATMLQGVGDAVQFEVSIGNYGNKFDNTCLPLASTTQFSRAVFDGCQYYYLPWGNVKPVVILSSYWEDCGHRTDAQNLLARAADRLAANLELVHLAQKAQRSASALDSLVAQMLDELMVDCSQPLVDVTQKPNSTHLDQYLYHFRTTNLDQMVQAALKMKHEDSDLQMVLDQAEDWLSRLKSMVEEPQNSLPDVVIWMLQGDRRVAYARLPAREVLFSRNGASCCGKNCGRLQTIFLKSPQEEVAGPRIPAQIRVRLWLGLAVDEKEFNQTTEGRLSVFAETVSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMQ2</accession>
    <name>A0A6I9XMQ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Paralemmin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106543662</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915196.1">
        <property type="nucleotide sequence ID" value="XM_014059721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMQ2"/>
    <dbReference type="GeneID" id="106543662"/>
    <dbReference type="KEGG" id="tsr:106543662"/>
    <dbReference type="OrthoDB" id="9941155at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498:SF10">
        <property type="entry name" value="PALM2 AND AKAP2 FUSION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498">
        <property type="entry name" value="PARALEMMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013915196.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="16953" checksum="17E08CACDB64D0CC" modified="2020-10-07" version="1">MQGIPASSPAEEEARKRQSEEDELKVKKLEGNIHRLEQEIGKLECEESQISAKEQIILEKLKETEKSFEDLQKSFSHQDGDAVNYIYSQIPELTTLYSQATEAVPGKDRTSRVTETERFVVWSENTNENPQVKATALVSDMILHISLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNM3</accession>
    <name>A0A6I9XNM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">folate gamma-glutamyl hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GGH</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + (n-1) H2O = (6S)-5,6,7,8-tetrahydrofolate + (n-1) L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:56784"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="EC" id="3.4.19.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.9" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013915562.1">
        <property type="nucleotide sequence ID" value="XM_014060087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNM3"/>
    <dbReference type="GeneID" id="106543965"/>
    <dbReference type="KEGG" id="tsr:106543965"/>
    <dbReference type="CTD" id="8836"/>
    <dbReference type="OrthoDB" id="64220at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034722">
        <property type="term" value="F:gamma-glutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046900">
        <property type="term" value="P:tetrahydrofolylpolyglutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000024">
        <property type="entry name" value="Folate gamma-glutamyl hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015527">
        <property type="entry name" value="Pept_C26_g-glut_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315:SF20">
        <property type="entry name" value="GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315">
        <property type="entry name" value="PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51275">
        <property type="entry name" value="PEPTIDASE_C26_GGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026961971" description="folate gamma-glutamyl hydrolase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6 7">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615527-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013915562.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="36219" checksum="B9B717838FDF1C10" modified="2020-10-07" version="1">MAKGLQVRGAWGLLAFCCCSAVSFVLRNENERPIIGILAQETFENFQKFGSSYIAASYVKFIESAGARVIPVRLNRSNEEYDEIFRSINGILFPGGGVDLETSEFSRVAKIFYTKALEANDREDYFPIWGTCLGHELLTYLTSGENLLVRTNTNGFTMPLNFTQASNHSKMFQDFPEEIYELLASEPVSSHFHFWSLSMQNYTKNEKLRNFYKILTTNFHNVEFISTMEAYRYPIYGVQWHPEKNPFEWKNSTGIPHSSASIKVTFFLANFLVNEARKSNHHFSSKKAEADALIYNFNPLYTGTFSSFDQVYFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRA2</accession>
    <name>A0A6I9XRA2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">USP6 N-terminal-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106542089</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with EPS8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913208.1">
        <property type="nucleotide sequence ID" value="XM_014057733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRA2"/>
    <dbReference type="GeneID" id="106542089"/>
    <dbReference type="KEGG" id="tsr:106542089"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000010">
        <property type="entry name" value="Putative USP6 N-terminal-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000001">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000019">
        <property type="entry name" value="USP6 N-terminal like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF19">
        <property type="entry name" value="USP6 N-TERMINAL-LIKE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="12">
        <location>
            <begin position="21"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="309"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="468"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="719"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="575"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913208.1"/>
        </source>
    </evidence>
    <sequence length="795" mass="90824" checksum="678A5C92D87F87D4" modified="2020-10-07" version="1">MLKSWEKYKNTDKFHRRMYKGIPLQLRGEIWSLLLDVPKMKEEMRGYYNTLKTRARGISPDIRQIDLDVNRTYRDHIMFRDRYGVKQQSLFHVLAAYSVYNTEVGYCQGMSHITALLLMYMNEEDAFWALVKLLSGPKHAMHGFFIPGFPKLLRFQEHHEKIMKKFLSKLKQHFDSQEVNTSFYTMKWFFQCFLDRTPFRLTLRIWDIYVLEGERVLTAMAYTILKLHRKHLVKLQMEELVQFLQDTLAKDFFYDDDFVIEQLQSSMAELKRAKMDLPVAGKEDEFPKKLGKTPADSQPAMLNLTHLVNGQKTNSVAKEAPASQRAGEGRREGSPRRYGEGGQESSPTARIKRDREGSPTPRGERGRESSPNRQRRKNSLEKRQILRHQQKPVTPNAQSGLPKHDGETPGRKPEAQTEAEPKQKHNATANQNSNAVLTTGKEFVPRWIKPSDVKIIEKTTKITMEFKTRLPKQGWTGPAPAPEDEAQRFNPKQKTRVWDVDESKRGSNASQYDNVPEADLENGSSVEQAPERVVLQNPRSTIFSSGSRKEIEKGSSPPKISNSYAFSSGPRVPRYSSQSDASVVGSHTRQPPPTYNNPPVYQGYSPKHTTKDGNFTATQYNHGTRTSSATQLYVPDFPPETETHQMLSERPPRNYSALHQNLVVTPSSRVEVLPDEVFPSSPRGHRPAIPVVDYLPDHKKWADPPLAYRPGAYGQPWGGDGGRSHFGHLKRHPAFQPKPLEDHNFPTLSVDRPLRYRTASGELGPPSYQYPSSPGPDRHYRHQADGLSMNESVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRM4</accession>
    <name>A0A6I9XRM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lysozyme</fullName>
            <ecNumber evidence="3">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106544846</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 22 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013916717.1">
        <property type="nucleotide sequence ID" value="XM_014061242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRM4"/>
    <dbReference type="GeneID" id="106544846"/>
    <dbReference type="KEGG" id="tsr:106544846"/>
    <dbReference type="OrthoDB" id="17373at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16898">
        <property type="entry name" value="LYZ_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000001">
        <property type="entry name" value="Lysozyme C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001916">
        <property type="entry name" value="Glyco_hydro_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019799">
        <property type="entry name" value="Glyco_hydro_22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000974">
        <property type="entry name" value="Glyco_hydro_22_lys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407:SF28">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00062">
        <property type="entry name" value="Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00137">
        <property type="entry name" value="LYSOZYME"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00135">
        <property type="entry name" value="LYZLACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00263">
        <property type="entry name" value="LYZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00128">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51348">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="5" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027039035" description="lysozyme" evidence="11">
        <location>
            <begin position="21"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 22 (GH22)" evidence="12">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="259"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013916717.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="33475" checksum="3A33126E61FABA77" modified="2020-10-07" version="1">MKTPNLVLFFLIVAVARSNAIVFDICSFSAILKENGLEGFSGTTIAEWICLVFHISGFDTSALNVGPKASNYGIFLLSAKWWCDDFKTLYAPNYCNLSCEALRDDNITDDIQCAKRVVQRSNGFGAWTAWRKHCKGEDLKKYIVGCDLEEVTSSPPVPEKIFTSDQRPKSIHPGVDLLTTPFRDMYPKEPTLPLKKEPKSELPAMGSSKPTTSIREFPNTTFLSENLLKSNISVKEPPRFTLSTHSPLKHSVALGEALNSIPPKIPPKPTASINETSNSTLQAVDPRKYPIPSHNNTEPSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRP3</accession>
    <name>A0A6I9XRP3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Storkhead-box protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106537723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907395.1">
        <property type="nucleotide sequence ID" value="XM_014051920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRP3"/>
    <dbReference type="GeneID" id="106537723"/>
    <dbReference type="KEGG" id="tsr:106537723"/>
    <dbReference type="OrthoDB" id="10020110at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040126">
        <property type="entry name" value="STOX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437:SF2">
        <property type="entry name" value="STORKHEAD-BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437">
        <property type="entry name" value="WINGED HELIX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907395.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15463" checksum="1923D31C7796E3A3" modified="2020-10-07" version="1">MPRRLDKFLQIAPHSLAIVLSPCGGDEAAPLEQAIRAQREGDADGEAGRGSRAEGEPAADSELGRHHIGYEIFADFKQGNMQHFWNQKVTAAVAETFFLGWIDEQVLLIQGKEEHLEALREGWMRRALKPPAGFQIKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRW0</accession>
    <name>A0A6I9XRW0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipoxygenase homology domain-containing protein 1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106537765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907475.1">
        <property type="nucleotide sequence ID" value="XM_014052000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRW0"/>
    <dbReference type="GeneID" id="106537765"/>
    <dbReference type="KEGG" id="tsr:106537765"/>
    <dbReference type="OrthoDB" id="5322100at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052970">
        <property type="entry name" value="Inner_ear_hair_cell_LOXHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901:SF7">
        <property type="entry name" value="OXYGEN-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901">
        <property type="entry name" value="PROTEIN CBG12474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="145"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="416"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="550"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907475.1"/>
        </source>
    </evidence>
    <sequence length="612" mass="69308" checksum="810C9862302453CC" modified="2020-10-07" version="1">MKQELWLLGKTNKESLTKDVHGIWDVTVIAGEMSDNYGNSKLILTVCDDKGTSAAVFIPKGSFKRAKIYQTSLELDKKFNKICKIRLEVEDSDRETWHCHEVKLQNKKNKETLEIPCYRDFPDNKGCTVVEFPILITGTRFLTVKQYILYISTDSAPKSGTDSDVYVTLKGTIGDTGRRKLFKNEQGCFSEGKVDVFLIEAVDIGTLCQLIVEKGKGSAWHLQKVMVKEPQYEGKETLFISQTWLRDTNEKKKYASLTLNANEVLDKGSTTASLLSSQNMKSEGLWKISFTTCREDSSKAFEEFGENITKLIWVFYGNRGKSEPISLMSKTGIQADDKLMFDVYFPSDLGTLYKVKIGVHLLEESIWQLFHHCKIQNTVTLDTFSLSLNETFPLLNRDQWLEFPIEWPLRKALSVITYDVKIFCSDIVSKISMVPISLYVYGTNGDTEDRRLLLSPSSTIEKGEIGQSFTGHIDAVDLGKLYKIVLLIGSTSSCKLAIKALHLKENLKQEPVYIFEVNETFHLDIDKPEIRREIPVSLVTKEDNGSENLTEHIIKVYTGDKRGAGTDANVHIILFGDMDTSHLIQLKTSLDHRDPFEKGKVSNIAQVNISFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRX3</accession>
    <name>A0A6I9XRX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Myosin-15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">MYH15</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916506.1">
        <property type="nucleotide sequence ID" value="XM_014061031.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106544684"/>
    <dbReference type="KEGG" id="tsr:106544684"/>
    <dbReference type="CTD" id="22989"/>
    <dbReference type="OrthoDB" id="312459at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000004">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000006">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000007">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF69">
        <property type="entry name" value="MYOSIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9 15" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="18">
        <location>
            <begin position="30"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="17">
        <location>
            <begin position="83"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="15">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="841"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1194"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1312"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="176"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013916506.1"/>
        </source>
    </evidence>
    <sequence length="1733" mass="199429" checksum="3DBE3FF4222210D2" modified="2020-10-07" version="1">MMDMSEFGEAAPFLRKSEKEIMVLQTVAFDGKKKCWIPDEKKAYIEAEIKESGAGKVTVETTDGKTLTVKEDDVQQMNPPKFDMMEDMAMLTHLNEASVLYNLYRRYSNWMIYTYSGLFCVTINPYKWLPVYKTEVVSAYKGKRRSEAPPHIFSVADNAYNDMLRNRENQSMLITGESGAGKTVNTKRVIQYFATVAAIGEPGKKSQPATKTGGTLEDQIIQANPALEAFGNAKTLRNDNSSRFGKFIRIHFGTTGKLSSADIEIYLLEKSRVIFQQPGERCYHIFYQILSGKKAELQDMLLVSTNPYDYHFCSQGVVTVDNLDDGEELMATDQAMDILGFVADEKYGAYKLTGAILHFGDMKFKQRPREEQAEADGTESADKAAYLIGVNSSDMLKGLVHPRVKVGNEYVTKGQSVEQVTYSVGALAKAVYDRMFKWLVTRINKTLDTKLPRQFFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGLDLQACIDLIEKPMGILSILEEECMFPKATDMTFKAKLYDNHLGKSPNLQKPRPDKRRKYEAHFELLHYAGVVPYNIGGWLEKNKDPLNETVVGIFQKSSNKLLASLFENYVSADSVDQSGEKKRKKGASFQTVSSMHKENLNKLMTNLRSTSPHFVRCIIPNETKTPGAMDAFLVLHQLRCNGVLEGIRICRKGFPNRVIYADFKQRYRILNPSAIPEDKFVDSRKAAEKLLGSLDIDCNQYRFGHTKVFFKAGLLGHLEEMRDERLAKILTLIQARARGKLMHTEFLKMLERRDALLVIQWNIRAFMVVKNWPWMKLFFKIKPLLKSAETEKEMANMKEEFLKLKEALEKSEARRKELEEKQVSLVQDKNDLLLQLQAEQDTLADAEERCDLLIKSKIQMEAKVKELTERVEDEEEMNSELTSKKRKLEDECSELKKDIDDLEITLAKVEKEKHATENKVKNLTEEMAALDETISKLTKEKKSLQEAHQQALDDLQVEEDKVNTLSKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESVMDLENDKLQLEEKLKKKEFEISQMSSKIEDEQAIVLQLQKKIKELQARIEELEEELEAERAARAKVEKQRSDLARELEELSERLEEAGGATSAQLEMNKKREAEFLKLRRDLEEATLHYEATAATLRKKHADSMAELGEQIDNLQRVKQKLEKEKSELKLEVDDLSSNMEQLVKGKGNAEKLCRTFEDHLSEAKTKVDEMTRVINDLTAQKGKLQTENGKLLDSSSLNGVSHCVSLLHASWAEEISDLTDQISEGNKNLHELEKMKKQTEQEKAEVQLALEEAEGALEHEESKTLRFQLELSQIKADFERKLAEKEEEIDNFRRNQQRTIDTLQSTLDAEAKSRSEAVRLKKKMEGDLNEMEIQLSHANRHASEATKATRILQAQIKELQVQLDDSTHLNEDFKEQLAVTDRRNTLLQSELDELRAVLDQTERSRKLAEQELLEATERVNLLHTQNTSLINQKKKLETDVTQIQTEVEEAIQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQKRLDEAEQIALKGGKKQIQKLESRIRELENDLEVEVRRNTESQKGVRKYERRIKELTYQAEEDKKNLTRMQELIDKLQLKVKSYKHQAEEAVNASKKIYFTGIGRANKNILKYYTKYSVNMLGGMLKERGYKEKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRZ3</accession>
    <name>A0A6I9XRZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein tweety homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TTYH2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Probable chloride channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the tweety family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916822.1">
        <property type="nucleotide sequence ID" value="XM_014061347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRZ3"/>
    <dbReference type="GeneID" id="106544936"/>
    <dbReference type="KEGG" id="tsr:106544936"/>
    <dbReference type="CTD" id="94015"/>
    <dbReference type="OrthoDB" id="187568at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072320">
        <property type="term" value="F:volume-sensitive chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07912">
        <property type="entry name" value="Tweety_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF6">
        <property type="entry name" value="PROTEIN TWEETY HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04906">
        <property type="entry name" value="Tweety"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 13" id="KW-0868">Chloride</keyword>
    <keyword evidence="9 13" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013916822.1"/>
        </source>
    </evidence>
    <sequence length="535" mass="59891" checksum="42FDA09FFAE8A91D" modified="2020-10-07" version="1">MLLAARAEYLAPWWTSWLHGLPHLSFSLRPVPNAFRPRDDDYQQSLILLALTGVLFLGLNLVFLGIYLICLSCCKKREESETKKPNSCCITWTAVVAALMCCAAVGIGFYGNSETNDGIYQLIYALDNANHTLSGVDSLVSGTRTQMKIVLEQHVARLNEIFATHSEYVQTLKFMQQMADNIVLQLSGLPVWSDVTVDLSMIANDVSYVEYYRWLSYLLLFILDLVICLIACLGLTKHSRCLLITMLCCGLFTLCLSWASFAADVSAAVGTSDFCVSPDKFILNMTKSDLSAEIVQYYLYCRQSPTSPFQQALTIYQRSLTTMQIQIQGLVQFAVPFFPTAKKDLLDMQKLLNYSEINLHQLTAMLDCRGLHKDYLDALVGICYDGVEGLLYLTLFSLMAAVSFSTIICAMPRAWKHLTSRDRDYDDIDEEDPFNPQARRIAAHNPNRGQLRSFCSYSSSLGSQTSLQPPAQTISNAPVSEYMNQAVLFGGNPRYENVPLIGRGSPPPTYSPSMRATYLAVNDDFRRQPSNEFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTV7</accession>
    <name>A0A6I9XTV7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox protein Hox-B9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HOXB9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917482.1">
        <property type="nucleotide sequence ID" value="XM_014062007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTV7"/>
    <dbReference type="GeneID" id="106545451"/>
    <dbReference type="KEGG" id="tsr:106545451"/>
    <dbReference type="CTD" id="3219"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009954">
        <property type="term" value="P:proximal/distal pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000018">
        <property type="entry name" value="Homeobox A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017112">
        <property type="entry name" value="HXA9/HXB9/HXC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970">
        <property type="entry name" value="AGAP004664-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970:SF2">
        <property type="entry name" value="AGAP004664-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="195"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="197"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="31"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="101"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013917482.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="30145" checksum="EAFA6B97528B41C7" modified="2020-10-07" version="1">MSHPSSGLGYLPLPFHHPGWRSSPHYDFFSRKNSSPGLYGPGRKDQDSSCLEVEADKFSPSCQEEDPLHELERKESVLFIRQSRTPKATFRPVSLPKLSLTTCPKQVSGQSSSTAEGNLSRSSFPPSPSYFADGRNCEVSVRLKPDYRPRPVGSKREDGDKEEEKADKKDGLQKSFSTLDETSPGNPAANWLHARSSRKKRCPYTKYQTLELEKEFLFNMYLTRDRRHEVARLLNLSERQVKIWFQNRRMKMKKMNKEQGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV27</accession>
    <name>A0A6I9XV27_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BLOC1S2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919974.1">
        <property type="nucleotide sequence ID" value="XM_014064499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV27"/>
    <dbReference type="GeneID" id="106547364"/>
    <dbReference type="KEGG" id="tsr:106547364"/>
    <dbReference type="CTD" id="282991"/>
    <dbReference type="OrthoDB" id="244061at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919974.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16286" checksum="B210F093FC461634" modified="2020-10-07" version="1">MAAPAASLEDGLEARRPGSTQEPVVETAEEATEPAEADINELCTEMFNKMAIYLTGELTATSEDYKLLENMNKLTSLKYLEMKDIAINISRNLKDLNQKYAALQPYLEQINQIEEQVAALEQAAYKLDAYSKKLEAKYKKMEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVY7</accession>
    <name>A0A6I9XVY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SKIV2L2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013918282.1">
        <property type="nucleotide sequence ID" value="XM_014062807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVY7"/>
    <dbReference type="GeneID" id="106546042"/>
    <dbReference type="KEGG" id="tsr:106546042"/>
    <dbReference type="CTD" id="23517"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18024">
        <property type="entry name" value="DEXHc_Mtr4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13154">
        <property type="entry name" value="KOW_Mtr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000141">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.300:FF:000001">
        <property type="entry name" value="Mtr4 exosome RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000004">
        <property type="entry name" value="Superkiller viralicidic activity 2-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="142"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="399"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013918282.1"/>
        </source>
    </evidence>
    <sequence length="998" mass="112888" checksum="2C2FE0754BCD8B91" modified="2020-10-07" version="1">MADAFGDELFSVFEDDGASASGLKETKGTPEAITSNKSGKQRDGKPVVPAKIQAKKKRDAEFSGADITDLGKRPKLDDISTDDLNLADVMPKVKVQAVETIEGCTHEVALPADMDFVALKARTGKAAKEYPFILDAFQREAILCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVGLMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRNSERGVVWEETIILLPDNVHYVFLSATIPNARQFAEWICHLHKQPCHVIYTDYRPTPLQHYIFPAGGDGLHLVVDENGDFREDNFNTAMQVLRDAGDLAKGDQKGRKGGTKGPSNVFKIVKMIMERNFQPVIIFSFSKKDCEAYALQMTKLDFNTDEEKRMVEEVFNNAIDCLSDEDKKLPQVEHVLPLLKRGIGIHHGGLLPILKETIEILFSEGLIKALFATETFAMGINMPARTVLFTSARKYDGKDFRWISSGEYIQMSGRAGRRGMDDRGIVILMVDEKMSPTIGKQLLKGSADPLNSAFHLTYNMVLNLLRVEEINPEYMLEKSFYQFQHYRAIPGVVEKVNNLEQQYNEIEIPNEENVIIYYKIRQQLAKLGKEIEEYIHKPKYCLPFLQPGRLVKVKNEDDDFGWGVVVNFSKKSNVKVNSGELDPLYVAEVLLHCSKESLKNSATEATKPAKPDEKGEMQVVPVLVHLLSSISSVRLYIPKDLRPLDNRQSVLKSIQEVQRRFPDGVPLLDPIDDMGIKDPGLKKVIQKIEAFEHRMYSHPLHNDSNLETVYKLCERKTQIAVDIKAAKRELKKARTVLQMDELKCRKRVLRRLGFATSSDVIEMKGRVACEISSADELLLTEMMFNGLFNDLSAEQATALLSCFVFQENVSYFFHRSIRANVLQQNKLKMKGSTDFCLCTFPHSGSIIRCMRRLEELLRQMCQAAKAIGNTELENKFAEAITKIKRDIVFAASLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW77</accession>
    <name>A0A6I9XW77_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Complement C4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106545859</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Precursor of non-enzymatic components of the classical, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Putative humoral mediator released following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway). While it is strongly similar to anaphylatoxins, its role is unclear. Was reported to act as a mediator of local inflammatory process; however these effects were probably due to contamination with C3a and/C5a anaphylatoxins in biological assays.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918016.1">
        <property type="nucleotide sequence ID" value="XM_014062541.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106545859"/>
    <dbReference type="KEGG" id="tsr:106545859"/>
    <dbReference type="OrthoDB" id="6359008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006956">
        <property type="term" value="P:complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00017">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02896">
        <property type="entry name" value="complement_C3_C4_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000013">
        <property type="entry name" value="Complement C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.690:FF:000002">
        <property type="entry name" value="Complement C4 isoform-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.20.50.160:FF:000001">
        <property type="entry name" value="Complement component 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1940:FF:000001">
        <property type="entry name" value="Complement component C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.91.20">
        <property type="entry name" value="Anaphylotoxins (complement system)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000020">
        <property type="entry name" value="Anaphylatoxin/fibulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018081">
        <property type="entry name" value="Anaphylatoxin_comp_syst"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048847">
        <property type="entry name" value="C4_MG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054587">
        <property type="entry name" value="CO4A-B_CUB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF86">
        <property type="entry name" value="COMPLEMENT C4-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01821">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21145">
        <property type="entry name" value="C4_MG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22661">
        <property type="entry name" value="CO4A-B_CUB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00104">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47686">
        <property type="entry name" value="Anaphylotoxins (complement system)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01177">
        <property type="entry name" value="ANAPHYLATOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01178">
        <property type="entry name" value="ANAPHYLATOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0765">Sulfation</keyword>
    <keyword evidence="5" id="KW-0882">Thioester bond</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026999685" evidence="9">
        <location>
            <begin position="24"/>
            <end position="1711"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphylatoxin-like" evidence="10">
        <location>
            <begin position="690"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="11">
        <location>
            <begin position="1564"/>
            <end position="1709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013918016.1"/>
        </source>
    </evidence>
    <sequence length="1711" mass="191455" checksum="109A2799B5465511" modified="2020-10-07" version="1">MSFQSRFLLWLSWMGVLIAMSHQAPRLMMVAPSVVTVGTEVRIVLQVDDAPSAVSGNVYFQNELNNKKCSEQMPFRLQSDPPGPSVQDVTLKVTQQFFDSCGLSRQKRDRYVQLVAYSSQLNTPIQSLNLRWSAREGYLLLQTDKPIYTPRQKVNFRVFPLDQKLRPTNNPVVITVKNPRGFQVKKIERSALKTVINDHLTIPDVAEPGIWTITAHFLRTQGYNTTTEFEVKKYVLPHFEVKIVPEQKYILVTGQQTSNLQINIQANFFYGKGVTGTAYVRFGVVGENKEKVYISGLKYQITIEDGEGSLILQRSDLVEKVGRPLQDLVGTSLYIAASVIETASGELEEQELTSVKFISSPYSVDLSKTKRYFVPNAPYEVLARITLPDGSPAPNLPVRFRTSITGAQSPMDEEQNTDEQGNVNYRINVARGSSSVTVMLTAGTESPAEVTLTAKATRSASGNYLIIENRKSYGLNIGDTHTIDLTPVGSIAFTRIYYMVLSKGDIISVSSIPRGSHLAASIHITPNLMPTFRVVAFYRLGDEIVSNSIWLEVVARCEGKLEIRSSSNVDRLKPQSRVSLTLNTDDKSFVSLSATDAAVYALNGKNRLSQGKVFEAMKSYDLGCTAGGGEDSLGVFADAGLSIRAGEQRSPLRKAHGCEENASRKKRSLSFQQEIDKKVSKYQTPNQVKCCKGGIILLRNARSCEERAKRIQNTECREVFLDCCRYAAKLRRQSWGSHGLARVDDEDEEDYFDDDFITLRRFFPESWLWKTFVVDKTLTELFHLPDSITTWEIQAVSVSLAKGICVSEPFKITVFQDFHISLRLPYSVKQFEQIELRPVLYNYLPDPLNVLVYLEPVEGICSPATFGPARKRKVEVPGNSAIPVPFVLVPTGANDIPITIIAAGEWGIGDKVSKKLRVEKEGAISVEEYTIPLDNQGDLIRSVTISGDLPSNAIPDGDFKMSVRLTGSIPADTLQSSLDPDGISSLLRVPHGCAEQTMVLMAPAVSAMQYLDQTEQWLLLKPESKEVALANLRTGYTRILTYRKNDGSYGAWIDTPSSTWLTAFVVKILSLARIYQDVENAGIRGSVQWILEQQQSDGSFTDSRPVYHREMQGCLGGLRGGVSLTAFVTIALKQALPLYEVKEEDDEAERQKQEQLSRLKNSLERATAYLATSLSGQSLGPYPMAISSYGLALASQDKSAIALAGSRLMQLSLEDTNSTMIFWLAGEDQRTKVPSAISVEATSYALLYLLKQNNAAVAAKVSRWLTEQRNYGGGFRSTQDTVVALEALSSYWISTFKEEGNELTVSLTVPGRHLPTQISFGRSNDPIQEELQFSLGKDIDVKVEGKGKGTLTVLKQFHVLAVQNTSCQTLGLEVTVSGSIRRNAEVEDYYYEYEDDLPPTSPPASKPLSRIELFDARQRRKRETKDTEQSRHDVTYEVCFWRQRGARVSGMVIVDISLLSGFQPDEVDLVQLQEVPDQYINHWETQGQRLLLYFDQAPQTGRECIAFRAKQVVSVGKLQPASATVYDFYEPHQRCSIFYGAPNKPQYVSALCSEDVCQCAEGACPRLKRTLEDAITEENRMNFACYSPRVHYAFLVRVERESRESAFRVYDVKIKAPLQFTTDSRIEIGQIRRFVVREACKTRLAAGKEYLLMGRDGETRDSDNRPQYLLDKNSWIEELPDLRRCKATQYRNTCSHLESFTTSFVINGCRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWF4</accession>
    <name>A0A6I9XWF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106547791</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920524.1">
        <property type="nucleotide sequence ID" value="XM_014065049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWF4"/>
    <dbReference type="GeneID" id="106547791"/>
    <dbReference type="KEGG" id="tsr:106547791"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="35"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013920524.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="35023" checksum="91FABE2BA113353C" modified="2020-10-07" version="1">MNITEFIVLGFGNVGQWQSLLFFVFLVIYIVATAGNVLIVTLVAVDQHLHTPMYFFLGNLSCLETFYISNIVPKMMAGLLTDKNTISFRNCFVQFFFFSSLMATECYLLSAMSYDRYLAICKPLHYTKSMTTRICIQLAASSWISSLIAGVFLLSVMLQLTFCGPNEIDHYFCDTYPIMKLSCSDISFVELISFILAFAFTLPPFLLTLTSYIYIISTILKIPSTIGRKKAFSTCSSHLIMVSLFYGTIMIVYGLPKRKILSDVNKIFSFLYTVLNPLANPFIYSLRNNEVKDAIKRLSRKLMSPKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWV5</accession>
    <name>A0A6I9XWV5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spatacsin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SPG11</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920684.1">
        <property type="nucleotide sequence ID" value="XM_014065209.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547932"/>
    <dbReference type="KEGG" id="tsr:106547932"/>
    <dbReference type="CTD" id="80208"/>
    <dbReference type="OrthoDB" id="2018754at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008088">
        <property type="term" value="P:axo-dendritic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048489">
        <property type="term" value="P:synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028103">
        <property type="entry name" value="Spatacsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028107">
        <property type="entry name" value="Spatacsin_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13650">
        <property type="entry name" value="SPATACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13650:SF0">
        <property type="entry name" value="SPATACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14649">
        <property type="entry name" value="Spatacsin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spatacsin C-terminal" evidence="1">
        <location>
            <begin position="1928"/>
            <end position="2219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920684.1"/>
        </source>
    </evidence>
    <sequence length="2276" mass="258254" checksum="E37769157AB68594" modified="2020-10-07" version="1">MLSWIHNGLYSVLSISSLQVLSFKNGRAILLLNNFVIAHLIFPGGELGEEVEGCFRLDLPPQTLERTVNVTFCRGILFLLDETGWIHIFGASDGAYLAHISVALYQSRGQEERKQDMPFPLGQLSISYDLSTAVVVSHSSCVAAVDLNLYFREFPDHLFCKRSFENLSAQQLEGVDEDDLNSSSYSMKFLQPSFQTDRSWKTHVLSLDDTRADNYFPHLCSDVELPWYQHLLHLEPMKSKALDTSAASLFQEVAFVLSSSRGKDNKSTEERRWKNVHLGDLEKRGSLDCKHVTGFHVLFTASSESEGLTLVLWDLVTQGITQSCVGKNSFFVEYTREEPLWLVLSETGLFLFLFGFTQEEFLTRLIIYGSASTVDSLCCLNRWGRCSIPVHALEAGLENRQLDTVDFFLKNKENLFSLSTASSLQDEPANIMSDFYLKSVEELRPALDLLCKAIQENDVETQSKHFSEQLLNLTLSFLNKHLREIFTHREELDENMLQCVDIFTSYIIKLRTFMIRFPHKPSGAMSQPWDSEDNLPQMEQSHWEKFDSEQAVTEALLSNRVPEAQIFFRLTHNPAQDLGQLTWIGLEMAYKSLLKDDINEASRLLRNMGLSVKQQLHKICFYTTDYHVRDFLVSVLQDEDVLPETEKEMVDFVHEVENIYSGTFQTQEKNAACCRSWRKEADCIDVTSLDSFLNCTQDRIHNREHRMMLNWAQGWNPSTRERILLPARAQEGEFQFCNPEALWMYLSSWHDWENIRWWISDSQLEGSPAKWPPLPLEAIGQSALCSPYLRNEVLDTLARNGTFVPAELEDFEGLLQRTACAGGVMQEPHPLPDYVSAGGLDFHTCFVLYCLERGLDHLLYSYLDYYRLFSSDCLILNDTDLREAHPWFEFLVQCWDIANHPGDTDRIFQASLANAQISIPSNQASVSTMLLEGRTLLALATTMFAGGGIDQMVQKGDGREKVDAQLFRMALAPYPKLRAALFPQTTPQGISPSDLSLYHLLQCLAPFDSTKLFGWQSANTLAIPDICSDLPHFSCSALVSKHAIIEHLDFSYYLYHERPSFAFGAFLAQQLSKSNRPKELVQQVAKEASSVALSLFYAPSLAATSVCFLEMLGLSSLELRVDIKAANVILRFMSRREEPQHHSIRQSLVEKLGELADGEKAAAEDLLVCLEEAVWDKVDHQEIKKTSSEARNHWSLVIQFCQLHHIKLSTSYLRACARSKEWLQFVTEAQMYSYQPAEVIPLLRDFPLPLQDHLELALGHLQPPRQGGLQGKEQKSQGLFQVLLQSQEQPCPWRHLLAASVKHHLPVLSVLAAGFQDASLLPCLCVWIITSLDSSAASEVIGHIEGRAETHQWGLPDLALLWQWLLKRQKARILLHGFQLFLEDSPLLIMLEVYELCMDYKNYTQAKIKLLTFQDNLSKLQAQGELPVLPGPWLEMQASFLLDLMLHQCRTEYEFRKLLQLLADAGTALLHGPDLKKLSALGAILKDSPTCISRTLLRSYSCEGFREECRRILEQLQESSSFSVARRVAELAALPVDNVVIQEVLQNLLLLKQVGHWTQKRTRVEFWKKCHETYVQNAISNQAASGFFSAQADLVSEGLEDERAPSISERQLLLTLAGYWLARSDPVPVGDLERIEREIWRCRIRRQGQMEARGSPQISISSEPSFDSLAKEFCFSKVAALNTPKFLELRAFPSQDATPVGLSEAEVASLAFLIGRLLDEGSVHEVARVCRYFSFYSRDVALILHCRTLALGEEAQGCFHPQIQALLAAEDSQRRREAAAEKKRLSSSSLDSWSSMGGSSPDSGVVGSLRTLVAECVHGRNYCRQTLYLYQLSKELGCSFSEIAVHDSEKLLQAILSSQQPDWYKKAQAFITNQGLEPGAVAELVAEEITRELLEPLEGRGKQTPALNPAEESQRFLQLAKLCQDHTLVGLKLLEKVSSVPHGELACTVELLILAHNCFSLTCHMEGITRVLQASRLLTDDHLAPEEEFGLVVRLLTGIGRYSEMTYIFDLLHEKHYFEVLMRKKLDPNGTLKTALLDYMKRCRPGDSEKHNMIALCFSMCREIGENHEAAANVQLKLIESQPWAESLQDVPSLKKLLMKALTLFLDATESYSKDFCVCQSLRCKRLTRLITLQLHFLASPHKTQLINLRRESLLPCILALPRFYQAAVVAEAYDFTPDWSEVLYQQVILKGDFNYLEEYKQHELLRTGTFEEITHKVKQQAANESAVRNLKQLLTHCEDIYVYYKLAYDNQFYDVVHLLLNDAQTGCCLNDLLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX61</accession>
    <name>A0A6I9XX61_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nuclear pore membrane glycoprotein 210</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NUP210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918752.1">
        <property type="nucleotide sequence ID" value="XM_014063277.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546407"/>
    <dbReference type="KEGG" id="tsr:106546407"/>
    <dbReference type="CTD" id="23225"/>
    <dbReference type="OrthoDB" id="361283at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1080:FF:000007">
        <property type="entry name" value="Nuclear Pore complex Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058779">
        <property type="entry name" value="Ig_NUP210_13th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057586">
        <property type="entry name" value="Ig_NUP210_16th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055098">
        <property type="entry name" value="Ig_NUP210_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056897">
        <property type="entry name" value="Ig_NUP210_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056898">
        <property type="entry name" value="Ig_NUP210_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055099">
        <property type="entry name" value="Ig_NUP210_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056899">
        <property type="entry name" value="Ig_NUP210_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045197">
        <property type="entry name" value="NUP210-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055094">
        <property type="entry name" value="NUP210_Ig15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055095">
        <property type="entry name" value="NUP210_Ig_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019:SF2">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN GP210-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02368">
        <property type="entry name" value="Big_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26181">
        <property type="entry name" value="Ig_NUP210_13th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26183">
        <property type="entry name" value="Ig_NUP210_14th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22959">
        <property type="entry name" value="Ig_NUP210_15th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25354">
        <property type="entry name" value="Ig_NUP210_16th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22963">
        <property type="entry name" value="Ig_NUP210_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24991">
        <property type="entry name" value="Ig_NUP210_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26182">
        <property type="entry name" value="Ig_NUP210_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24935">
        <property type="entry name" value="Ig_NUP210_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22962">
        <property type="entry name" value="Ig_NUP210_7th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26184">
        <property type="entry name" value="Ig_NUP210_8th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24902">
        <property type="entry name" value="Ig_NUP210_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22957">
        <property type="entry name" value="NUP210_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00635">
        <property type="entry name" value="BID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="Invasin/intimin cell-adhesion fragments"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1527"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="3">
        <location>
            <begin position="802"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1564"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1564"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1582"/>
            <end position="1594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013918752.1"/>
        </source>
    </evidence>
    <sequence length="1605" mass="176369" checksum="5C9FD891A6D6A556" modified="2020-10-07" version="1">MPSDQYELQLQNNTLSSGGDTAWPIARLDQATCTVTALQRGQANLVLSHKSVSMQGASKLPNGTLFVVDPGYLGFSIHPGERWVLETERFYEITIEVYDKSSNKVYLSENIRIDVYFPKEYFEVMRSSLNGSYHYVKAIKEGHIVLTAKLTSIVDQDGGVHTFSVPAQNQQEVDIYAPIVLQPSILTFPWQAKAGAYQYRILAYGGSGNFTWTSSQQNVASITIKGVMTTGNAIGISVIHARDVQNPLHHGEMKVYVIEPSDMKFTPCPVEAHVGEILDLPLKIIGVMDKETNEVVTLSDCSQFDLVVNIENQDVFNPFQGRLQPTPEYCSGIKVKAETQGYSSLVVSYTHDHIHLSASITIAAYLPIKIIDPISAALVTLGSSKDILFEGGPRPWIQEPTKFFRDTNTEHPENVGLHLLGPPTSRNSFQHWARATCKALGDHVISLTIGNKPTVTNPFPAVESVDVTLICATPSQFSLALVSTDPEMDVSCPFLHQDKQGVPISIFRNPVLKLDVYDHQGNKFDNFSSLTITWESTDHLLASVEARMPMELSMKDHGNSQKKMQGLQTVLVHHKPGATIISATAVGYQHIHLINNEVKKPYEALLPVSAGIELILVKDVKMSPKEVTIYNHQAVKAELIINGGSGYFFINTSVVDIVKIVYEEGKDIALIYPLYPGSVSVMIHDLCLASSTIAKTDVYVSNIQEVYVGIVDKVEIGKNVKAYVRVLDASKKYFHSKNFKFMDLTLKAASQIISLKALGETPDEYTAVFLVQGMAIGQTSVTGVVTDRYGEKIYSVPQQIEVFPPFKLIPKKVTLLIGAVIQITSEGGPQPQFNIIFSIHDPSIASVNNSGFITGKAVGNSTILGMVQAVHTEAGKVVVASQDKVEVEVIQLEAVRIHAPITRMKTGTQMPVYVMGIAGNQTPFSFGNAVPGLRFYWSVTTRDILDVKSRFNEASLQLSAENNFAVDVYGKGKGKTGLKVVVKVLDPSAGQFSHMAKELTDEIQIQVFEKLVLLGIDLGQILMSTNSFFKVQTNRDGAASLTYRVLDSQDKAPVVEIDESGYLSSHSHIGLSTIEIISQESFGINQTLVVAVKVAPISYLRISMNPIFHTKNHEPLLALPLGITLSLTIHFHDNYGDTFHSQNSMLHFAVNRDDFLQVGKGATNNTLVLRTMNIGLSLLKVWDAEHNIADYVPLPVQHAISAELIDVIVGDVICLTSSLVNQDGLTGTWSSSSDSILQIDSKAGVAVARNSGSVIIYYDVSGLCKTYREVLINEPKKIVVTHVSEMKTNLQDTSKVILQIGARSKNLKGECSSAQIEVIEKMKPQSMINCQLNFNNNVFDFQASEIVAAEPMFDTALGQYTCSIRILRLADKQLKQLTMSKTAITITPSLQGSHSLEQISTEVPFNPGFYANQTEFILTNHYTSSDVKIFGAAEVLKNLEVRSSSPLMVVFEKDRSYGLPSYVIYTVSLSDPEIASKTNLNAVLTISSPMTDQSLAIPVTVTYVSDRTLPMKYNTSLFQHFLDSYQVIFFTAFALLAGMAVMIIVHYTLLSSNEQQIHPAFITKTSPQRPHTGSPVTPTAPFSPVSSSPQKSSPNRLWSPPYSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXI5</accession>
    <name>A0A6I9XXI5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA repair protein RAD52 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RAD52</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918516.1">
        <property type="nucleotide sequence ID" value="XM_014063041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXI5"/>
    <dbReference type="GeneID" id="106546221"/>
    <dbReference type="KEGG" id="tsr:106546221"/>
    <dbReference type="CTD" id="5893"/>
    <dbReference type="OrthoDB" id="206565at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010792">
        <property type="term" value="P:DNA double-strand break processing involved in repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.80:FF:000001">
        <property type="entry name" value="DNA repair protein RAD52 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004585">
        <property type="entry name" value="DNA_recomb/repair_Rad52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00607">
        <property type="entry name" value="rad52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918516.1"/>
        </source>
    </evidence>
    <sequence length="392" mass="43953" checksum="79848864C5E62874" modified="2020-10-07" version="1">MARDGNGNTSDTPNTWFGQCQYTADEYQAVQAALRQRLGPEYISSRQAGGGQKVCYIEGHRVISLANEMFGYNGWAHSVTQQNVDFVDLNNGKFYVGVCAFVKVQLKDGAYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALKCFGNALGNCILDKDYLRSVNKLPRQMPPDLDLGKVKRQDFDPTIEKARYNSCLQKGSEDQKLFSLSTPSKSRKSATPLNAIEQNQNRISLPLDYDATHLRKIRQKQLQEKFRQEMQEKQQIQSDVSACRAKDQEASNLPALECNSSVPPESVVDEFLADDPELWDFPLEATDHNTAAVPAQCLRSPLGQHCMMTRSRTPQRINNQQSNIKPSSWHLSSSPKSYLAAEQSPYRKGQGMKKRRLEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYF0</accession>
    <name>A0A6I9XYF0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor IIB</fullName>
            <ecNumber evidence="4">2.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">General transcription factor TFIIB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GTF2B</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Also exhibits autoacetyltransferase activity that contributes to the activated transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013919222.1">
        <property type="nucleotide sequence ID" value="XM_014063747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYF0"/>
    <dbReference type="GeneID" id="106546790"/>
    <dbReference type="KEGG" id="tsr:106546790"/>
    <dbReference type="CTD" id="2959"/>
    <dbReference type="OrthoDB" id="25790at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990114">
        <property type="term" value="P:RNA polymerase II core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20551">
        <property type="entry name" value="CYCLIN_TFIIB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20552">
        <property type="entry name" value="CYCLIN_TFIIB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000008">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000019">
        <property type="entry name" value="transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000003">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF77">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cyclin-like" evidence="20">
        <location>
            <begin position="88"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="20">
        <location>
            <begin position="182"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00056112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013919222.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="31478" checksum="6787A6D2642278DF" modified="2020-10-07" version="1">MICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDTQNPLLSDGDLTTMIGKGTGAASFDEFGNSKYQNRRTMSSFDRAMMNAFKEIASMADRINLPRNIVDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLGLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPADFRFDTPVDKLPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZD6</accession>
    <name>A0A6I9XZD6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">METTL5</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915968.1">
        <property type="nucleotide sequence ID" value="XM_014060493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZD6"/>
    <dbReference type="GeneID" id="106544261"/>
    <dbReference type="KEGG" id="tsr:106544261"/>
    <dbReference type="CTD" id="29081"/>
    <dbReference type="OrthoDB" id="419617at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008988">
        <property type="term" value="F:rRNA (adenine-N6-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="3">
        <location>
            <begin position="46"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00041374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013915968.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24810" checksum="25CC3E22EA6A3EF3" modified="2020-10-07" version="1">MKKLKLKELESCLQQVDVFENPKLLLEQYPTQPHIAACMLYTIHNTFDDIENKIVADLGCGCGILSIGSAMLGAGLCIGFDIDANALEVFNRNAEEFDLTNVDMVQCNVCSLPDKMSKTFDTVIMNPPFGTKHNKGMDMAFLKTAVQMARIAVYSLHKTSTRQHIQKKADEWNIGMEVVAELRYDLPASYKFHKKKSVSIRPLLIKSKPIIFLLNNRVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZJ4</accession>
    <name>A0A6I9XZJ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein transport protein Sec24C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SEC24C</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916038.1">
        <property type="nucleotide sequence ID" value="XM_014060563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZJ4"/>
    <dbReference type="GeneID" id="106544317"/>
    <dbReference type="KEGG" id="tsr:106544317"/>
    <dbReference type="CTD" id="9632"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000020">
        <property type="entry name" value="protein transport protein Sec24D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000003">
        <property type="entry name" value="SEC24 homolog D, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF5">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC24C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="10">
        <location>
            <begin position="509"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="11">
        <location>
            <begin position="586"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="13">
        <location>
            <begin position="835"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="12">
        <location>
            <begin position="931"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="9">
        <location>
            <begin position="1051"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="137"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013916038.1"/>
        </source>
    </evidence>
    <sequence length="1181" mass="128895" checksum="D2E6C475615A94E4" modified="2020-10-07" version="1">MSVNQQAHAGPPYGQSQPGYANYMQPDYGGQQMQSVPASQYSAHNGPGTGYQQAGLPQGYYPSLGFPSKNPPVSLDSDASFAPNFLGPVRAPPTSSGASASTSGTLIPSEQPAYSQFGQGDVRNGISVSTVPVQRSSASQQFMAGPAPPSMAHTPAFQQYGLPPTSTQQLTSQVAEMQIGRGSSSVPSPAGLGYGPPTSVTPASGSFTAPGSGQYMLYSGQGPPPPNISHGLPPTTLSQGLPIAQPPISGPSALHTSAQPPLFAPPPSAAGVGISSYPAAAGISRPPTMQGALLPGQPVTRLLTSQPNHVTSPPPQPLMTGPPPGPPVTGLQGPPPVHPSQQGYALQQNGSFGQNRGTQPVYGAAFPGPANYGSQPAAPPPPKRLDPDSIPSPQLNELPPQQKPRHRIDPDAIPSPIQVIEDDRNNRGSEPFITGVRGQVPPLVTTNFLVKDQGNASPRYIRCTSYNIPCTSDMAKQSQVPLAAVIKPLAALPSEETPPYLVDHGESGPIRCNRCKAYMCPFMQFIEGGRRFQCCFCSCVTEVPSHYFQHLDHTGRRVDYYDRPELSLGSYEYLATVDYCKNNKYPNPPAFIFMIDVSYNAVRSGLVRLICEELKTLLDYLPREGTTEESAIRVGFVTYNKVLHFYNVKSSLAQPQMMVVSDIADMFVPLLDGFLVKVNESRTVFTSLLDQIPEMFADTRETETVFAPVIQAGMEALKAADCAGKLFIFHTSLPIAEAPGKLKNRDDKKLVNTDKEKTLFQPQTNFYNNLAKDCVAQGCCVDLFLFPNQYLDVATLGVVPYQTGGSVYKYTYFQVETDQERFLNDLRRDVQKEIGFDAVMRVRTSTGIRATDFFGAFCMSNTTDVELAGLDCDKTITVEFKHDDKLREESGALLQCAVLYTSCAGQRRLRIHNLSLSCCTQLADLYRNCETDTLINFLAKFAYRGVLNNPVKTVRDSLINQCAQILACYRKNCASPSSAGQLILPECMKLLPVYLNCVLKSDVLQPGSEVTTDDRSYIRQLISSMDVAETHVFFYPRLLPLTKIDYSSDSLPIAIRNSEERLSKGDIYLLENGLNIFLWVGVNVQQGLIQNLFGVSSFSQINSSMCTLPVLDNPFSKRVRSTIDLFQAQRSRYMKFIIVKQEDKLDLLFKHFLVEDKSMNGGASYVDFLCHMHKEIRQLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y089</accession>
    <name>A0A6I9Y089_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Microfibrillar-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MFAP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MFAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910150.1">
        <property type="nucleotide sequence ID" value="XM_014054675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y089"/>
    <dbReference type="GeneID" id="106539786"/>
    <dbReference type="KEGG" id="tsr:106539786"/>
    <dbReference type="CTD" id="4237"/>
    <dbReference type="OrthoDB" id="9947781at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048048">
        <property type="term" value="P:embryonic eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008673">
        <property type="entry name" value="MAGP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16485">
        <property type="entry name" value="MICROFIBRILLAR-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16485:SF3">
        <property type="entry name" value="MICROFIBRILLAR-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05507">
        <property type="entry name" value="MAGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027064907" evidence="10">
        <location>
            <begin position="20"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="11">
        <location>
            <begin position="147"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910150.1"/>
        </source>
    </evidence>
    <sequence length="177" mass="20025" checksum="CB7DCC1CEC71786C" modified="2020-10-07" version="1">MRTLLALLLLCLPALLAQGQYDLDDPAYPEPPYAPYEIESQDYYDYAGNDLSPQTPEEHFQSQQQNQQEVIPAPTPDISETEPTEPGPLDCQEEQYPCTRLYSIYKPCKQCLNEICFYSLRRVYVINKEICVRTVCAHEELLRADLCRDRFSKCGIMATSGLCQTMATSCARSCGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0T5</accession>
    <name>A0A6I9Y0T5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Alpha-synuclein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106539950</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the synuclein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910355.1">
        <property type="nucleotide sequence ID" value="XM_014054880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0T5"/>
    <dbReference type="GeneID" id="106539950"/>
    <dbReference type="KEGG" id="tsr:106539950"/>
    <dbReference type="OrthoDB" id="9900372at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:chemical synaptic transmission"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of dopamine secretion"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:synapse organization"/>
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        <property type="term" value="P:synaptic vesicle endocytosis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Alpha-synuclein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Synuclein"/>
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        <property type="entry name" value="Synuclein_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820:SF5">
        <property type="entry name" value="ALPHA-SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SYNUCLEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01387">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASYNUCLEIN"/>
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        <property type="entry name" value="Synuclein"/>
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    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="95"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="117"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361225"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013910355.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="14571" checksum="EFA340DD70C7C5A5" modified="2020-10-07" version="1">MDVFMKGFNKAKEGMVAAAEKTKQGVAEAAGKTKEGVLYMGSKTKEGVVHGVTTVAEKTKEQVSNVGGAVVTGVTAVAQKTVEGAGNIAAATGFGKKDQLAKQNEEGLTQEGNTDPADPENEAYEMPPEEEYQDYEPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y150</accession>
    <name>A0A6I9Y150_9SAUR</name>
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        <submittedName>
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        <name evidence="6" type="primary">LOC106547454</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
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    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="14"/>
            <end position="157"/>
        </location>
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    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
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            <begin position="166"/>
            <end position="270"/>
        </location>
    </feature>
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        <location>
            <begin position="262"/>
            <end position="284"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013920111.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="32193" checksum="FEDD07356F8DC1E2" modified="2020-10-07" version="1">MCGEPPSSASRANNRLRMTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGEAPNGRCVVRPQKTSRYCWSVRVDVLERISQSSGFMPPVLGTRRPDFYGIVNLHRYDYEQFSLYADPLLNLFPLSLSPEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEGKPEDDALKYLGGPILSPKPTNPQGRCHNGVSMEPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y242</accession>
    <name>A0A6I9Y242_9SAUR</name>
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        <submittedName>
            <fullName evidence="3 4 5">Glucose-induced degradation protein 8 homolog</fullName>
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        <name evidence="3 4 5" type="primary">GID8</name>
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    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Toxicofera</taxon>
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            <taxon>Colubroidea</taxon>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="3 4 5">Skeletal muscle</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014065607.1"/>
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        <property type="nucleotide sequence ID" value="XM_014065608.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014065609.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="CRA_dom"/>
    </dbReference>
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        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
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        <property type="entry name" value="CTLH_C"/>
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        <property type="entry name" value="LisH"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00757">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="1">
        <location>
            <begin position="63"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013921082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013921084.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="26799" checksum="D29500BC582BE977" modified="2020-10-07" version="1">MSYAEKPDEITKDEWMEKLNNLHIQRADMNRLIMNYLVTEGFKEAAEKFRMESGIEPSVDLETLDERIKIREMILKGQIQEAIALINSLHPELLDTNRYLYFHLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECLTEMERTLALLAFDNPEESPFGDLLNMMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKKVKYPKMTDLSKGTIEEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2Q3</accession>
    <name>A0A6I9Y2Q3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IQ calmodulin-binding motif-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">IQCB1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920871.1">
        <property type="nucleotide sequence ID" value="XM_014065396.1"/>
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    <dbReference type="GeneID" id="106548081"/>
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    <dbReference type="CTD" id="9657"/>
    <dbReference type="OrthoDB" id="8178106at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028765">
        <property type="entry name" value="IQCB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15673">
        <property type="entry name" value="IQ CALMODULIN-BINDING MOTIF CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15673:SF2">
        <property type="entry name" value="IQ CALMODULIN-BINDING MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013920871.1"/>
        </source>
    </evidence>
    <sequence length="602" mass="70331" checksum="F158E3C5B27C6598" modified="2020-10-07" version="1">MLREMKPLPVDSRILSLTAQITESPDQDIPVLLLKLKEILTSASPGSNETKKIKQDLYYCDLVQYCLLVLKQDYTKVTGGWTTAAQIAEILSQCCVGLEVKTEREEFYSKQLPSAIDSLLFLGRRLQAQFVRAIKDEKRNEFLHCFRTVTDAICWLCRGHIHLVENVLQNKHFQQLLTTDDDETTTQTLSLLQNILRTNSKALVQITEEALYFLLDELIYKISSTTNPARGNATVKLLLLITESDAQLVIRVNARYKGLHTLLSKQWTGKGFDKNLNQLLDLLDAENSSSYDPQRMHQAACLIQASWRGYQTRKRLKQLPKAITILQRKFRAKREQELQSLRRQKEEEHLRQQLQLQRQRAMRLFHERQLTLLEIVHAGQIDKYMHEMKEKAALTIQRYWRAFKARRNFHQQKRNLKEYKAAVLIQRAVLKFLEKKRRKESSLWKQPESLSDEQRILLQQKVESYTKLHPVIFLQASAMSEEMSRELHHQAQEKLAHYMLRKRKDQKAKEHREALLAQVHTDVDQLLSAPSLSECTMRDLNVFMSRSVPVVAKAKQSHNTILKYMQWPWWKKLEEGFFKEEVDLVDPTTAELETLFIGGSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2T9</accession>
    <name>A0A6I9Y2T9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Glycine receptor subunit alpha-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106545432</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917458.1">
        <property type="nucleotide sequence ID" value="XM_014061983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2T9"/>
    <dbReference type="GeneID" id="106545432"/>
    <dbReference type="KEGG" id="tsr:106545432"/>
    <dbReference type="OrthoDB" id="407674at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19060">
        <property type="entry name" value="LGIC_TM_GlyR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000003">
        <property type="entry name" value="Glycine receptor alpha 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="14">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="15">
        <location>
            <begin position="89"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013917458.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="32587" checksum="BB357223B7018878" modified="2020-10-07" version="1">MDLKNFPMDIQTCTMQLESFGYTMNDLIFEWEKEQEAVQVAEGLTLPQFILRDEKELGYCTKSYNTGQFTCIEVKFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVGLGITTVLTMTTQSAGSRASLPKVSYVKAIDIWMAVCLLFVFAALLEYAAVNFVSRQHKELLRLRRSQRRQRMEEELVRESRFYFQGYGLGHCLQHMKSGTAMESPIYSPPAPATLLREGEMLRRRYVERAKRIDTVSRAVFPFTFLVFNIFYWVVYKVLHQAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y320</accession>
    <name>A0A6I9Y320_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dynein heavy chain 8, axonemal-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106540618</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911275.1">
        <property type="nucleotide sequence ID" value="XM_014055800.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540618"/>
    <dbReference type="KEGG" id="tsr:106540618"/>
    <dbReference type="OrthoDB" id="9026607at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001221">
        <property type="entry name" value="Axonemal dynein heavy chain 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000543">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000009">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000044">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000003">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000003">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056759">
        <property type="entry name" value="DYH2-5-8_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25007">
        <property type="entry name" value="DYH2-5-8_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2028"/>
            <end position="2143"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2244"/>
            <end position="2397"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2570"/>
            <end position="2718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="964"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="989"/>
            <end position="998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013911275.1"/>
        </source>
    </evidence>
    <sequence length="3052" mass="349335" checksum="9306C312C1DBF5C3" modified="2020-10-07" version="1">MENAADSNPDPDQDEPAAQAENQPPPPEIIEPTPEPPEDVSKQKDADLADRSHLKPPVRTSGETATRRASKFRRSNSGVQSLQETLKEKQARYKEAREGRKMKVNAAYKYIFEILSDQLGLDIGTVEELILDGPTLQAFDMFFAKDGNRTLKFIYQEGEAPGIECGRAIPGILKGTKMMKLYVHESHDKFKGQCLFFVRARNDVPVNIKTMNEDIYYSSLDGSEGLLIAIKNCLVTVFLPAILATSNWGALNQTKQGELEKQNFIETLNRYLAFLDGARVSIQGTVQLKKIDSIDFSKFQSFEEVTAAAANPDIIHQLEDALMIWYKQIEQVLIESEQMRKEADDSGPLTELEHWKRMSAKFNFIIEQIKGQPCKAVINVLNVSRSKIIKLWRELDARITDTANESKDNVRYLYTLEKVCQPLYNYDLVSMAHGIQNLINAIRMIHSVSRYYNTSEKMTSLFIKVTNQMVTACKAYITDGGFSRVWEQGTPTVIQKIKDCIFLFKEYQKSFHKTRKQILETSGEKTFEVSEMYIFGKFEAFCKRLEKITEMITIVQTFSALSMSTIEGIDIMAIKFKNIYQSVQKKKYDILDPRKTEFDVDFADFMTKIENLEVQIQTFMGACFVKILSSQHALQLLQRFQKLNMPCLQNEISYTIGRILQHYVAELESIKKLYQIQKDNPPLARNMPPVAGKILWVRQLFRRISEPINYFYKNSDILASVEGKAVVRLYNKISYVLVEFEVLYHTAWIKEISQLEYAMQVTLLVRHPETGKLLVNFDPKILEIVRETKCMIKMALEVPEQAKRIVKIEGEVKGNKLCLENILHCYENLCQEIPTVFVNLMGPKMKKVESVLRQGLTILTWSSLTLNSFFQDVDEVIKMFNQLLKKINDLCEMQIDLTLKDISNTLLLILPESATKVEDLLSRNEVYTKEWAEILNNKSRHIEDAVQELISIFEQIYEVKHVKKPAPKKAHSPEGKHVAFGGGEEERLSSGSSIMISSEETEDSEKEDEFKKECKEVVAYFSHQLLDSLQKTTRLSLDALKRRVFVSSSSNFGRLPYLPQTTVKPEDVVTFLKAEVHLAIPHVVMVPSLDDIQQALNRMIQLTLDVSRGVAHWGQHHLLKSNNQGESNVPASPSILGKLQKREEKTQEDQLPIRKLRNFYPGVAEHKDISKLVVLLSSAVNSVRKAASEALQDFQKYKALWIEDRDAKVQQFLANNPSLTEIRSEILHYATFEQEIEDLKPTVSVGPIELNTGPMKLALSIEAKAWKMLLCRYLNEEYKKKMQDMISFVTEYLKKLSRPIRDLDDVRFAMEALSNIRDKEIEMDMIMGPIEEAYTILYRFEVEVTKEESESVDTLRYSFNKLQSKAASVQDELIQVQPKFKGQLLEAVEIFREDVSNYEVAYETEGPMVPNTPPQEASNRLQIFQANFDELWRKFITYSSGEQLFGLPVTDYEVLHKNRKELSLLQKLYGLYDTVMNNISGYYEILWGDVDIEKINAELLEFQNRCRKLPKGLKDWQAYLDLKKRIDDFSESCPLLEMMTNKAMKLRHWNRISETTGHPFDVESDSFCLRNIMEAPLLKHKDDIEDICISAIKEKDIEAKLAQVVDSWAYQVLSFSAFKGRGELLLKGTESSEIITMMEDSLMILGSLLSNRYNAPFKKDIQNWVFKLSTSSDIIEEWLIVQNLWVYLEAVFVGGDIAKQLPQEAKRFQNIDKSWIKIMQRAHENPNVVSCCVGDETMGQLLPHLHEQLEVCQKSLTGYLEKKRLLFPRFFFISDPALLEILGQASDSHTIQPHLPGLSDNINEVEFHPKDYDRIMTVVSREGEKIPLDTPVNAKGPVELWLMDLLKMQQSSLHCVIRAAFYQISDPGYQLLNFLYHFPAQVGLLGIQMLWTHDSEEALNSAKDDRKIMSVTNQKFLDILNTLISQTTHDLTKFDRVKFETLITIHVHQRDIFDDLVKMHIKSVTDFEWLKQCRFYFKEDLDQTLVSITDVDFVYQNEFLGCTDRLVITPLTDRCYITLAQALGMNMGGAPAGPAGTGKTETTKDMGKALGKYVVVFNCSDQMDFRGLGRIFKGLAQSGSWGCFDEFNRIELPVLSVAAQQIYIILTARKERKKQFIFSDGDTVDLNPEFGIFLTMVHYDFGLRNILSVLRTLGSQKRARPDDSEMSTVMRGLRDMNLSKLVDEDEPLFLSLINDLFPGLVLDSNTYVDLQAAVANQVEEAGLVNHPPWNLKLVQLYETSKVRHGLMTLGPSGSGKTAVITILMRAMTECGYPHREMRMNPKAITAPQMFGKLDTATNDWTDGIFSTLWRKTLKAKKGENIFLILDGPVDAIWIENLNSVLDDNKTLTLANGDRIPMSPTCKLLFEVHNIENASPATVSRMGMVYISSSALTWRPILQGWLKTRTPQEADVLLSLYDKAFEAAYTYMKLNLFPKMELLECNYIMQSINLLEGLIPSKEEGGIVCLAHLHKLFVFGIMWSLGSLLELDSREKLEAFLRNHESKLELPTFDKGESHTMYEYFVSDCGDWDHWNKKVQEYIYPTDSIPEYSSILVPNVDNVRTQFLMDTIAKQHKAVLLIGEQGTAKTVMIKGYLKKYDPEEQLSKSLNFSSATEPGMFQRTIESYVDKRMGSTYGPPGGRKMTIFIDDINMPIINEWGDQVTNEIVRQMMEMEGMYSLDKPGDFTTIVDVQLIAAMIHPGGGRNDIPQRLKRQFTVFNCTLPSNASIDKIFGIIGNGYFHSCRNFKAEISDMIMKLVSAGRILWQWTKIKMLPTPSKFHYIFNLRDLSRIWQGMLTIKSEECTSIHTLLSLFKHECTRVIADRFITEDDEEWFDRNFSRVIEEHVDPNLVSQVTAEPYFVDFLRDMPEPTGEEPEDFVFEAPKIYETVPSLEFLADKLQFYQRQYNENVRGGLLDLVFFKDAITHLVKISRIIRTACGNALLVGVGGSGKQSLSRLASFIAGYKIFQITLTRSYNVTNLTDDLKNLYRVAGGEGKGITFIFTDNEIKDEAFLEYLNNLLSSGEVRISNHYRCCCYADYYILKDKGSPCTYVLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y399</accession>
    <name>A0A6I9Y399_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PTRH1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the PTH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013917723.1">
        <property type="nucleotide sequence ID" value="XM_014062248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y399"/>
    <dbReference type="GeneID" id="106545613"/>
    <dbReference type="KEGG" id="tsr:106545613"/>
    <dbReference type="CTD" id="138428"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="entry name" value="Peptidyl-tRNA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224:SF1">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="Peptidyl-tRNA hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917723.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="39836" checksum="1C26E8029EFE822E" modified="2020-10-07" version="1">MGLAFLYYLLLLLLLLLVLYYCSLKALTLISNFQAASYIFAYFKGFYSTIIPLLKYELHLKKSRASAPEKDGAGNLPARPPAIGHQPDDVTLTEPGGKGSDAELGLPPPPFLKKSLRPAPEVGLWEGGGEEAMMRPQPLGQLLRWALGHRSDELQTAQKRVMVAGLGNHTFPGTRHSVGMAVLNHLASKLDVADQWKRDKQCCADVAMARLGEAELLLMKPRKFMNLNGISVVTAAERYNLDIEEIYLVHDDLDRPLGKLSLKLGGSARGHNGVRSCISSLRSDRMVRLRVGIGRPTEKASVEDYVLSRFTDAEQEVLPPVLERAANMLLQHIQQNCGERELPGSRDGRVSPPPKTEGRGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5G6</accession>
    <name>A0A6I9Y5G6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">KPNA6</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913125.1">
        <property type="nucleotide sequence ID" value="XM_014057650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5G6"/>
    <dbReference type="GeneID" id="106542044"/>
    <dbReference type="KEGG" id="tsr:106542044"/>
    <dbReference type="CTD" id="23633"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000001">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000013">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="126"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913125.1"/>
        </source>
    </evidence>
    <sequence length="538" mass="60074" checksum="403C15025CFE2E61" modified="2020-10-07" version="1">METMASPAKENNYRMKSYKNKALNPEEMRRRREEEGIQLRKQKREQQLFKRRNVELINEDAAMFDSPLVDSLVSSTIGSEGVITMEMVKMLFSEDPDLQLLTTQRFRKLLSKEPNPPIDEVISTQGVVDRFVEFLKRSENSTLQFEAAWALTNIASGNSLQTKIVIEAGAVPIFIELLSADFEDVQEQAVWALGNIAGDSSACRDYVLNCGILPPLLLLLTKSTRLTMTRNAVWALSNLCRGKNPPPEFEKVSSCLPVLSRLLFSSDSDLLADACWALSYLSDGPNEKIQAVIDSGVCRRLVELLMHSDYKVASPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSSPKESIRKEACWTISNVTAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIKYLVNLGCIKPLCDLLTVMDAKIVQVALNGLENILRLGEQEAKQSGTGLNPYCSLIEEAYGLDKIEFLQSHENQEIYQKAFDLYEHYFGIEDDDSTLAPQVDEAQQQFIFQQPEAPMEGFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6S8</accession>
    <name>A0A6I9Y6S8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 182</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TMEM182</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM182 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914255.1">
        <property type="nucleotide sequence ID" value="XM_014058780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6S8"/>
    <dbReference type="GeneID" id="106542912"/>
    <dbReference type="KEGG" id="tsr:106542912"/>
    <dbReference type="CTD" id="130827"/>
    <dbReference type="OrthoDB" id="9942154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026763">
        <property type="entry name" value="TMEM182"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32012:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32012">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 182-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914255.1"/>
        </source>
    </evidence>
    <sequence length="185" mass="21306" checksum="AEDC27A06B2CC00C" modified="2020-10-07" version="1">MKLSVGIFFGGFFGALGILFFLVAFGTDYWLLATEVGKCSEDVNRTRMIMFHHEGFFWRCWFDGKIDENPSSMLKFWYTNQSPSKNCTHAYLSPFPFLRDSHNSTTYNSAISILFSLVVVLYVIWVQAVADLKNYEAQKKIDCSSYSIYVRYGWSFILAPVGIFFAMFSGMLFLLVGRTIYLHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8D2</accession>
    <name>A0A6I9Y8D2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc transporter ZIP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Solute carrier family 39 member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Zrt- and Irt-like protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SLC39A3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29352"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916720.1">
        <property type="nucleotide sequence ID" value="XM_014061245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8D2"/>
    <dbReference type="GeneID" id="106544849"/>
    <dbReference type="KEGG" id="tsr:106544849"/>
    <dbReference type="CTD" id="29985"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF221">
        <property type="entry name" value="ZINC TRANSPORTER ZIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013916720.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="33651" checksum="8C43D95B7779EDBE" modified="2020-10-07" version="1">MEVVAAKVLCLLGTLALMLAGALLPVKVLEADPERAQRSRRALGLCNALGGGVFLATCFNALLPAVRQKLQEVLKLGNVTTDYPLAETIMMLGFFLTVFVEQLVLTFRREKPSFIDLETFNAGSDVGSDSEYESPFIEPSWGGHGPRADRGRHSHGLAIYELSRSSPLRLFSLVFALSAHSIFEGLALGLQEEGDKVMSLFLGVAIHETLVAVALGINMAKTGLKIKEASKLAAAVSLMIPLGIGIGMGIESAQNVASSVASVILQGIAGGTFLFVTFFEILAKELEDKKDRLLKVLFLVLGYAALAGLVFLKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8I4</accession>
    <name>A0A6I9Y8I4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sister chromatid cohesion protein PDS5 homolog A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PDS5A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916980.1">
        <property type="nucleotide sequence ID" value="XM_014061505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8I4"/>
    <dbReference type="GeneID" id="106545049"/>
    <dbReference type="KEGG" id="tsr:106545049"/>
    <dbReference type="CTD" id="23244"/>
    <dbReference type="OrthoDB" id="200660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000064">
        <property type="entry name" value="Sister chromatid cohesion protein PDS5 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF2">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN PDS5 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916980.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="43868" checksum="46FEF8E580545684" modified="2020-10-07" version="1">MLHLPELHEGPVEACEEGNFLDGGGGGDIGGSARMDFPSAQPKPPPDGKIIFYPPGVKEITDKITNDEVVKRLKMVVKTFMDMDQDSEDEKQQYLPLALHLASEFFLRNPNKDVRLLVACCLADIFRIYAPEAPYTSHDKLKNLNKQAFDLAKILLKRTVQTIEPCIANFFNQVLVLGKSSVSDLSEHIFDLIQELFAVDPNLLLSVMPQLEFKLKSNDGEERLAVVRLLAKLFGSKDSDLATQNRPLWQCFLGRFNDIHVPVRLESVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVTIITAGKRDLSLVNDQLLGFVRERTLDKRWRVRKEAMMGLAQLYKKYCLHAEAGKDAAEKVSWIKDKLLHIYYQNSIDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y966</accession>
    <name>A0A6I9Y966_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106545938</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 10">Cell membrane</location>
            <topology evidence="3 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918140.1">
        <property type="nucleotide sequence ID" value="XM_014062665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y966"/>
    <dbReference type="GeneID" id="106545938"/>
    <dbReference type="KEGG" id="tsr:106545938"/>
    <dbReference type="OrthoDB" id="10061722at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000003">
        <property type="entry name" value="Gap junction protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF117">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="13">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="14">
        <location>
            <begin position="176"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="107"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="255"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="340"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013918140.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="43134" checksum="1BB417AC61EA1111" modified="2020-10-07" version="1">MSWSFLTRLLEEINQHSTFVGKVWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTQQPGCENVCYDSFAPLSHVRFWIFQIIMIATPSVMYLGFALHRLSRQPPRKRRAPVVRHGAARDYEEAEDNGEEDPMIFEEIEPEKEVKEPESQKHDGRRRIKKDGLMTAYVLQLLARSAFEVGFLMGQYMLYQFEVNPSYICKSSPCPHTVDCFVSRPTEKTIFLLIMYAVSGLCLLLSICELFHLGCGGIRDALRGLKDDAPPPKPQYAQKDPSAPPTYHSLKKHPSKGQLTKEKLGYRGERMAAERYPVTSRLPSHELERLRKHLRVAQEHLEMAFQMQPEGIASPLRSSSPESNGLAAEQNRFNLAHEKEGTACERPSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9C3</accession>
    <name>A0A6I9Y9C3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Unconventional myosin-IXb isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MYO9B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918425.1">
        <property type="nucleotide sequence ID" value="XM_014062950.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546168"/>
    <dbReference type="KEGG" id="tsr:106546168"/>
    <dbReference type="CTD" id="4650"/>
    <dbReference type="OrthoDB" id="312459at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072673">
        <property type="term" value="P:lamellipodium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000008">
        <property type="entry name" value="Putative unconventional myosin-IXa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000009">
        <property type="entry name" value="unconventional myosin-IXb isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046987">
        <property type="entry name" value="Myo9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184:SF2">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="11">
        <location>
            <begin position="1011"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="12">
        <location>
            <begin position="1079"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="468"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="832"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1407"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="594"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="638"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="669"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="686"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="732"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="775"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="846"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1437"/>
            <end position="1449"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013918425.1"/>
        </source>
    </evidence>
    <sequence length="1466" mass="167189" checksum="2985634F1A50728B" modified="2020-10-07" version="1">MDYMRPDIVALLRSSDSTYVRELIGMDPVAVFRWAILRAAIHAMAVFAEGGRQRAEKTAGVIRHGPRIPLGELQHSNTPIERVYRCSVLDFSFDCSEDFDINAFEDIIASYESKKDLHEQIVRSIKGLPWQGEDPRRLLQSLSRYQQPRCHFLKSRGMKQKQIIPKNLLDSKSLKLIVSMTLHDRTTKSLLHLHKKKKPPSISAQFQSSLNKLLETLGKAEPFFIRCIRSNSEKRELLFDERLVLQQLRYTGMLETVRIRQSGYSAKFNFQDFIDQFQVLLPKNAQASKEEINALLNKLNLDTNSYQIGKTKVFMKEATRQVIQDTLHKEVIRKIIVLQSWLRMVLERRRFLRTRQAAITLQACWRSYCVVRRALEKENAAIYIQSVWRGYRERKSYQQQKKRVCLLQALARGYLQRKRFQQMVAEKKQEEIKQELLKQVNELLQPKEPISDQTAVELEVQLDKTSGLTAEDDDGAKNAHAESQSPPLSQKINRKKRENRRQRGLEHNKLQNQQVVLAFEGGAEVPPREDSPPRNTAEEEETQKLEQPIGDAAVSSSEMLPKCVEEEGAPGTDNHHFPLEGGTLAPSPSPGLKLESRVLKEDTNVQERLENPKIRTSQSFTYPERPTELDLAWPNNRQIHRSFRSHADKRKQQAVKDLGSPTSFQIQRYQDDPGKLRDKRERWQGKRQQVSGQNNLVSQSLEEKSIPAKPSSSAHLKKKKTASSLDDIPKITLSTSPSQPSLDTVEGEGNTQKKISPKKSEELPTTLSAVGLPSGPSQQMDAKSTFKSPLRRLLAKRTDKKNVKEYSEVYEDTSETIPLFSCMLPMEPTVTQKATEAPSGPPNHPPLEERQTKEFGKAKRNRTIKISKAKRAPEKWKAPFLREIANASELKYLDEFLLNKINDLHSQKSAIECLFFEATEKFRGNIKTMYSAPNEQIHVGYRDLMENYQLLVVNMAKQKEEKDVKLVLNLFQSLLDEFARSYAKKEEPEQHKSKAQKKKRKQEHVIVELNGHVFVNYQVNILQSCEQCCSYIWPMEKACLCSICKLTCHKKCLSKIQSGCTSSQGKKGDQDPEPRHFGVSVSVLTSEKNSVPIVMEKLLEYVEMHGLYTEGIYRKSGSANRMRELKQSLQTDPNSVLLENYPIHTITGIFKQWLRELPDPLMTFAQYNDFLRAVELPEKQEQLYAIYSVLEQLPQANHDTLERLIFHLVKVAVVEEVNRMSPNALAIVFAPCLLRCPDNLDPLTSMKDVSKTTTCVEMIIKEQLRKYKMKMDEICQLEAAESIALRRLSLLQQNKLWPVKLAFSSPCEGAPSPQTLGASGTVPKLGLLPEEEETDNREKELLIERIQSIKEEKEDITYRLPELDQRCSDEENVDSETSISTESLLEERVGCRDPEGSKEPAAQCISLSAKEENGSPKTLPFSAKVDPTPPRLSTTCGPSSLLLLKGSPLHQTPGDAHSQAQAVFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAF9</accession>
    <name>A0A6I9YAF9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Interleukin-17B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IL17B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920355.1">
        <property type="nucleotide sequence ID" value="XM_014064880.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tsr:106547653"/>
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    <dbReference type="OrthoDB" id="9896444at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020440">
        <property type="entry name" value="IL-17_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01932">
        <property type="entry name" value="INTRLEUKIN17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026951223" evidence="7">
        <location>
            <begin position="19"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013920355.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="22548" checksum="E692FA12C9E28E90" modified="2020-10-07" version="1">MEWAPKLLLISALCLSLALDATPVSKEQSRGTKGRRRGSAKAREQHLRAQKSSPDPVLRVNLGALTDYTFVEDYERSIQEMVNQLRNSSELLDSKCQVNLRLWMSNKRSLSPWAYRINHDASRVPVDIPEAHCLCAGCINPFTMQEDRTMVSIPIYSKIPVRRMLCEPSKPHRKKRKCHKEYKMVMETIAVGCTCIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBZ4</accession>
    <name>A0A6I9YBZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rho-associated protein kinase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106548729</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921648.1">
        <property type="nucleotide sequence ID" value="XM_014066173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBZ4"/>
    <dbReference type="GeneID" id="106548729"/>
    <dbReference type="KEGG" id="tsr:106548729"/>
    <dbReference type="OrthoDB" id="9046978at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072518">
        <property type="term" value="F:Rho-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901888">
        <property type="term" value="P:regulation of cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000072">
        <property type="entry name" value="Rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF28">
        <property type="entry name" value="RHO-ASSOCIATED PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="95"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921648.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19596" checksum="71DCBD700A009213" modified="2020-10-07" version="1">MNHQQPRQATKMSTSPPAAVSGEGHLVSRQRKLESMIRDPRSPVNVESLLDGLKSLVLDLDYPALRKNKNIDNFLNRYEKIVRKMRDLQMKVEDYDVVKVIGRGAFGVVQLVRHKITQKAYAMKLLSKFEMLKRSDSAFFWEERDIMAFANSPWVVQVWIFLVYKVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC64</accession>
    <name>A0A6I9YC64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CWF19-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CWF19L2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWF19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921712.1">
        <property type="nucleotide sequence ID" value="XM_014066237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC64"/>
    <dbReference type="GeneID" id="106548784"/>
    <dbReference type="KEGG" id="tsr:106548784"/>
    <dbReference type="CTD" id="143884"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000021">
        <property type="entry name" value="CWF19-like protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF5">
        <property type="entry name" value="CWF19-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cwf19-like C-terminal" evidence="5">
        <location>
            <begin position="153"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Cwf19-like protein C-terminal" evidence="4">
        <location>
            <begin position="285"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921712.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="45019" checksum="FA48EDC4C1371394" modified="2020-10-07" version="1">MGEFQKLEEMLPGVHTRTGKLSFWFPKTGFQEDHEAILVRMDKSGRAWPVNIPEETLDHVGRRRKNQMIPTHVDKERIRYFQDDDNLDLKDLIRNEKMSTSEDQNKLFMRMASKFSGKTDRDYYTLDDMFVSNAAKREESGKAEERERHQAIREHQRLAACMEKCPHCFGSSELPKHLIIAIGNKVYLSLPKYQSLTEGHCLIAPMQHYTAATVLDEDIWEEIQVFRKTLVKMFEAEELDCVFLETNLSISKQYHMIYECIPLPKEVGDMAPIYFKKAIMESDEEWAMNKKLINLGSKDVRKSVPKGLPYFSVDFGLQGGFAHVIENQYMFPRYFGKEIIGGMLDLEPRLWRKGLRENFEDQRKKVLHFAQKWKFYDFTKSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDH5</accession>
    <name>A0A6I9YDH5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C-type lectin domain family 10 member A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106549056</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922070.1">
        <property type="nucleotide sequence ID" value="XM_014066595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDH5"/>
    <dbReference type="GeneID" id="106549056"/>
    <dbReference type="KEGG" id="tsr:106549056"/>
    <dbReference type="OrthoDB" id="6133475at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051379">
        <property type="entry name" value="C-type_Lectin_Receptor_IMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746:SF9">
        <property type="entry name" value="CD209 ANTIGEN-LIKE PROTEIN C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013922070.1"/>
        </source>
    </evidence>
    <sequence length="249" mass="28228" checksum="7395F6E03CF6B40C" modified="2020-10-07" version="1">MSSPQGIYKRCEEPEEQGKKPSKKFETAGNFLPLRSQGGSGVSLLIFSLVTIAFLLLWIIIVAVMASKYSKIFKELEELRMNQTLLTKNDLKTEKKLQQIQSNHVSQETALNITLKKLEDDHNGLKKNVNAKIQEISDESGRSANMVINLINAVHQINASGCQVCPKGWLLNREKCYYFKMGSDAWSQAEKRCENDGSKLVIIEDYVEQKMQLQTKAAPCIDSESEISMEWKSDSNSWEIQGVRYANEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGW1</accession>
    <name>A0A6I9YGW1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein LOC106550202</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106550202</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923538.1">
        <property type="nucleotide sequence ID" value="XM_014068063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGW1"/>
    <dbReference type="GeneID" id="106550202"/>
    <dbReference type="KEGG" id="tsr:106550202"/>
    <dbReference type="OrthoDB" id="5982876at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052224">
        <property type="entry name" value="THAP_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927">
        <property type="entry name" value="AGAP005574-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="310"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013923538.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="47034" checksum="73B26C154EAB09B5" modified="2020-10-07" version="1">MNQEKWIPTKHQHLCSDHFAPSCFEYRWGVRYLKPDAVPTIFQTSDSPLKREGPGKPPANTPAKKLILECGGEWAAPAAETPACQEPDAMETLTIAIDPSVGATPVYVETQSSAPNLDFQALSGPLVGTVNLLPLVQIVEPLKAVTLAVASPAVPGQAPQEPPEQQVVDFVASLPPATLGALPSSSVGLAGQLCTEVALPCEVLAAGDQSSLRAVMAEPAVMAEQGALVIENVSIEPFLAGGPSVALPVLQEPTATTQMVAYFETIPTAPAATASSGLSPPETVLSSALSLPIVSTLPIVSNQAAPEASLSAEEAKLEEGSSSGDSSEEPLGEHRYHRHEGTTAELVEVVSGLQKKVMVLQQRHRRHCAKLEAMEGVVEQLWKENLVSEEKLKVLEMACLQSSTFMPEAGGAVAIICQDRDQALVYAMPQLPGEASETILQLEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGX0</accession>
    <name>A0A6I9YGX0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Deoxyguanosine kinase, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCK/DGK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923442.1">
        <property type="nucleotide sequence ID" value="XM_014067967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGX0"/>
    <dbReference type="GeneID" id="106550152"/>
    <dbReference type="KEGG" id="tsr:106550152"/>
    <dbReference type="OrthoDB" id="567086at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004138">
        <property type="term" value="F:deoxyguanosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF8">
        <property type="entry name" value="DEOXYGUANOSINE KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="3">
        <location>
            <begin position="11"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="65"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923442.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17874" checksum="617F6375CF495400" modified="2020-10-07" version="1">MDPPGEEGLRMYIFAKNLFEIGHMTEIEWIIYQDWHTFLLQTFGDQLALHGFLYLQAPPEVCFERLRCRSRPEEKEVQLSYLEQLHVQHENWLVKKTSVSHSGALRDVPVLILDVTKDFENDPNEQSKLIGQVNFFMKTLCSNSSPSISTTVCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGY5</accession>
    <name>A0A6I9YGY5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Fibrillin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106550157</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923451.1">
        <property type="nucleotide sequence ID" value="XM_014067976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGY5"/>
    <dbReference type="GeneID" id="106550157"/>
    <dbReference type="KEGG" id="tsr:106550157"/>
    <dbReference type="OrthoDB" id="9045857at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000097">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000009">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000003">
        <property type="entry name" value="Fibrillin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000003">
        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051145">
        <property type="entry name" value="GAS-SHBG-PROS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040:SF16">
        <property type="entry name" value="FIBRILLIN-2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040">
        <property type="entry name" value="LAMININ G-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="11">
        <location>
            <begin position="86"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="301"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="343"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="402"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="11">
        <location>
            <begin position="448"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="520"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013923451.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="61470" checksum="55922E09C134928C" modified="2020-10-07" version="1">MCTCPNGQVSPSCGSKSVQHCNIRCMNGGSCSDDHCLCQKGYTGTHCGQPVCENGCLNGGRCVAPNRCACTYGFTGPQCERDYRTGPCFTLVTNQMCQGQLSGIVCTKTLCCATVGRAWGHPCEMCPAQPSPCRRGFIPNIRTGACQDVDECQAIPGICQGGKCINAVGSFECKCPAGHKFNEVTQKCDDIDECSTIPGICEGGECSNTISSYFCKCPSGYATTSDGTGCIGEYIATVNFTQANLCQVYQNLCINGRCIPTPGSYRCECKKGFELDIRGECIEEYHGLCSSGPGITASGNDINECALNPDICPNGVCENLRGTYKCICNSGYEVDSTGKVCLDIDECAVNRLLCDNGLCRNTPGSFTCTCPKGFVYKPDLQTCEDPICAKGYSRIRGNQCDDVDECVVFPGVCTNGHCVNTMGSFICQCPSGMILDATGRRCIDIRLESCYLRHDDEDCTSPIGGRHRLDACCCSVGASWGPDCDECPIRSTPEYEALCPRGPGFSTRGEVVNGKPFYKDINECKMIPRLCTYGKCRNTIGSFRCKCDNGFALDSDERNCTGNQESRRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YID0</accession>
    <name>A0A6I9YID0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 186</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CCDC186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924123.1">
        <property type="nucleotide sequence ID" value="XM_014068648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YID0"/>
    <dbReference type="GeneID" id="106550694"/>
    <dbReference type="KEGG" id="tsr:106550694"/>
    <dbReference type="CTD" id="55088"/>
    <dbReference type="OrthoDB" id="5583482at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099518">
        <property type="term" value="P:vesicle cytoskeletal trafficking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038830">
        <property type="entry name" value="CCDC186"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18911:SF5">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18911">
        <property type="entry name" value="CTCL TUMOR ANTIGEN HD-CL-01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="646"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="249"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="708"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="810"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="661"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924123.1"/>
        </source>
    </evidence>
    <sequence length="841" mass="97369" checksum="054A8291E1B1C604" modified="2020-10-07" version="1">MDEHLPEQHNTPRMVNLSETDNESSNLPSVEEADKNYDIQDDVHIADARCEKSTLSIENKELILDPILSSIDLNEDGNAIEAQPAPNFNGDCCELKVKESKEADCLPSGQGEQHVLKTEDKNQSLTDLFQDHVKRPYGVCSESPYDTDYTKNLISNLENASSQEALLEEIESELLSTELLKNPTLSNGMCNTKATLAVFEKCIQDKYLQQEHTIKKLIKENKKHQELILDICSEKDNLKDELKKRTETEKQHLNIIRQLEVRIEDLQKEAKTVKDKLISQDAAAKNAIQQLHKEMAFRTDQANKKCEEARQEKEAMVMKYVRGEKESLDLRKEKETLERKLRDANKDIEKHTNKIKQLSQEKARLHQLFESKENETTRMSRDIEKLKEEVNSYVIKVKWAQNKLKAEVDLHKETKDRLKEATAKLTQAKEETDQIRKNCQEIIKTYQVKCLEDERLRTEDELSKYRELINRQKAEIHNLLEKVKTLDQLQEQHQRDEQEMNSWKEEVDNLNTLLSDFQQDIDGSRKRESELLVFTEKLTTKNAQLQSETNSLQMQLDKLTFMERESQNQLELINQARDELANKLKKEDELHRNKVQILEMELASQQKSFANLNTKAEELKDELMTQKRKHAANLKDLTKQLQQARRKLDNLENGSCDKEVSSMGSRSSSSGSLNARSSNEDRSPENTGSSVVVDHFPEVDKTILIERIVRLQKAHARKNEKMEFMEDHIKQLVDEIRKKTKIIQSYILREEAGTLSSEASDFNKAHLSRRGGIMASLYTSHPADSGLTLELSLEINRKLQAVLEDTLLKNITLKDNLQTLGTEIERLIKQQHELEQRLKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIH2</accession>
    <name>A0A6I9YIH2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DENN domain-containing protein 1B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106550602</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924012.1">
        <property type="nucleotide sequence ID" value="XM_014068537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIH2"/>
    <dbReference type="GeneID" id="106550602"/>
    <dbReference type="KEGG" id="tsr:106550602"/>
    <dbReference type="OrthoDB" id="206724at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.200:FF:000003">
        <property type="entry name" value="DENN domain containing 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000001">
        <property type="entry name" value="Putative DENN domain-containing protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040032">
        <property type="entry name" value="DENND1A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196">
        <property type="entry name" value="DENN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196:SF24">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="4">
        <location>
            <begin position="14"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924012.1"/>
        </source>
    </evidence>
    <sequence length="404" mass="45892" checksum="E28ECD719A6EA8D5" modified="2020-10-07" version="1">MDSRVKENPQTTFDLVIQVKCQASENEDPVVLWKFPEDFGDQAILQSIPKFCFPFDITRVTHVGQHFTFVLTDIESKQRFGFCRLTSGGKVCLCILSYLPWFEMYYKLLNTLAEYLAKEQEADLNDLLETWHNHSVPEANTPISLNLHSYFITPDLTGLPTVPESRNLTEYFVAVDVNNMLQLYASMLHERRIIITSSKLSTLTACVHGSAALLYPMYWQHIYIPVLPPHLLDYCCAPMPYLIGVHFSLMERVKSKSLEDVVMLNVDTNTLETPFNDLSNLPSEVVSALKSQLKKQSTATGDGVARAFLRAQVALFGSYRDALRYKPGEPIVFCEESFLKHRSSSTKHFLETAANLQLFKQFIEGRLAKLNAGRGFSDIFEEEISSGSGGSRHMLYYFLTTGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ09</accession>
    <name>A0A6I9YJ09_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arylacetamide deacetylase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106550531</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923939.1">
        <property type="nucleotide sequence ID" value="XM_014068464.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550531"/>
    <dbReference type="KEGG" id="tsr:106550531"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF28">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026826255" evidence="4">
        <location>
            <begin position="21"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="5">
        <location>
            <begin position="107"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013923939.1"/>
        </source>
    </evidence>
    <sequence length="264" mass="29756" checksum="A839D25AE027D892" modified="2020-10-07" version="1">MGRKFFCLLVASTLIAYYIYQPLPENVEEPWKLTLLEASIRSLNHAATIVEKLGLGRYMDVINLYMRFEYTAPTSDENVIVTDTEFSHVPVRLYIPTKQSDVLKRAVIFIHGGGWCTGSASMKAYDLLSRWTSERLNAVVVSVDYRLAPKYRFPLAFEDVYSVSKYFLQSSILEKYNVDPSRIAIAGDSAGGGLAAAVTQQILDDPDVKTKFKIQALLYPGLQTIDAELPSLQDNKNMPILPKTLVIKFLSEYFPSDDFFXSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJF0</accession>
    <name>A0A6I9YJF0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein PRRC2B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550907</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924372.1">
        <property type="nucleotide sequence ID" value="XM_014068897.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550907"/>
    <dbReference type="KEGG" id="tsr:106550907"/>
    <dbReference type="OrthoDB" id="1939715at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009738">
        <property type="entry name" value="BAT2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033184">
        <property type="entry name" value="PRRC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14038">
        <property type="entry name" value="BAT2 HLA-B-ASSOCIATED TRANSCRIPT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14038:SF4">
        <property type="entry name" value="PROTEIN PRRC2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07001">
        <property type="entry name" value="BAT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAT2 N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="436"/>
        </location>
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            <begin position="450"/>
            <end position="469"/>
        </location>
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            <begin position="478"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="534"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="800"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1078"/>
            <end position="1552"/>
        </location>
    </feature>
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        <location>
            <begin position="1568"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1640"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="216"/>
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        </location>
    </feature>
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            <begin position="320"/>
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        <location>
            <begin position="553"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="590"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="709"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="870"/>
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        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="946"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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            <begin position="1008"/>
            <end position="1040"/>
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    </feature>
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        <location>
            <begin position="1101"/>
            <end position="1117"/>
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        <location>
            <begin position="1172"/>
            <end position="1203"/>
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    </feature>
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        <location>
            <begin position="1230"/>
            <end position="1245"/>
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        <location>
            <begin position="1304"/>
            <end position="1321"/>
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    </feature>
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        <location>
            <begin position="1348"/>
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        <location>
            <begin position="1429"/>
            <end position="1438"/>
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        <location>
            <begin position="1484"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1502"/>
            <end position="1512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013924372.1"/>
        </source>
    </evidence>
    <sequence length="1665" mass="185284" checksum="2B923C21E841BD4F" modified="2020-10-07" version="1">MSDRSGQITKGKDGKTKYSTLSLFDKYKGKSVETVRSTVIPRHGLQSLGKVASARRMPPPANLPSLKSEHKGNDPNIIIVPKDGTGWANKQDPPEQKSSSCTAAQPRESLPQQDSQKSVSNLQKRNLSISQENTNSALGGVKSWAQLNGKPARVEGGSRGSSRLLSFSPEDFPTLKAAGEQDRSGKEKGTLDPSYGPGPSLRPQNVTSWREGGGRNSTSATSLSPFPTELGTKSSSTGEDVPTSANGADPKDLSLRPSLPARKGASQFMANTYQPPTYHDMLPAFMCSNPPAETPSLPERASFPLPSFCLEPRVPFRQHQPVDPDGKDKRLGPPRPVRLVRPPGERVPRPTIINAENLKGLDELDNDTEDGWAGIHDEVDYSEKLKFSEDEEEEDTLKDGRQKWNGWDPRRPPLLSSAESTDVHYPTEDPKTWNDSASLSRTIRKVSESLPGSRKLNGWSPLPEYQKPPLSCLRQQCSEEKEEKPPLRQKFVYSEMSEAVERARRRREEEERRAREERLAACAAKLKQLDQKCKLAQKAGDGPKHVESDEPRSSTSEKSAVSDNSHVTRRAVPEPHAQEPPSGYVDEETPAAAATAALRSSSEEELRESVSPMQEFGKLPKLLPPRFQRKQQDQLYKMQNWPPSQVYPPSSHPQRTFYPAHPQMLGFDPRWLMMPSYMDPRVPPGRPPVDFYPSSLHQSGMMKPAMQPDSMNGGSCPSEEQSCQSVGLQERKGEATPIWGPESFVPLPSKNCFVSQPKQTESETEEGLYTRVEDSYSAPSELSTQHDFFEEVGEDYLSSFEKKPQGNFERCVSPAERAPQERIFQPPECGSDLSDPCDRSLLNSPLEPGSIPDEKKKSEYNGWDISHFSKPLEEEPSTKEEVFQEDSLFGSEMWKKEGSSSKQANPGTGWTTPENRNGSGQPPQQQEPTSRTRRSGPIKKPVLKALKVEEKEKELEKVKVDGEETSHPSKEKGPPPKASEEINPGGVVHSTRYRSLDERCSPQTPIPENERRHEDNDKDSKEKETVTKDLEGKNPSREFAELPPTKRNNWIFIDEEQAFGGRGQGRGRGRGFREFTFRGRSNTVGCGGAAVASSGGGNLRGGYNSSSSSNVGGPPRSSRGRALRDFNQGEEFPRGKPRRRIASETHSEGSEYEELPKRRRQRASENNNEGSLVEREEDDLKKGDFHDSWRSNKIYSDDQDAKLRAPRAFGRSLPPRLSNSTYGRRGFVSKESSQWQSRSGGNTWQEHSHNAPLEAFAIRRPSERDYGQESYKASDSFSRRAFEEDYGGDPENVENRPFRRRRPPRQDKPPRFRRLRQEREPVSQWTPEEGGADLAACQWPRRAKLVTTDYNGPSNRRSPELSYQNTSDHANEEWETASESSDFSERRERRGGGGGNTENEALLDGGAPGSTLGEKRDLAKRSFSSQRPVVDRQSRKSDPVGFGEKAVRAGGNRPASYYENQQNVLQLRSKRSPDENGIPDNGSPGRNRSTYGSHSNLNGVESLEKRQEKELKPVVSEMSENVSPFELSYRGGVMDSRGAGPGAGAEVGPMNSEGFIEVLTKKQRRLLEEERRKKEQAAQAPAKGRVLQSRIPPRFAKKQNSLCLEQGDVSVPRNTLGTEIWETNSAALSVQSSGPDSWNKPIGTFIANEPGSAEVSGPFSHAKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJU0</accession>
    <name>A0A6I9YJU0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Inactive dipeptidyl peptidase 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DPP10</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924035.1">
        <property type="nucleotide sequence ID" value="XM_014068560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJU0"/>
    <dbReference type="GeneID" id="106550624"/>
    <dbReference type="KEGG" id="tsr:106550624"/>
    <dbReference type="CTD" id="57628"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF21">
        <property type="entry name" value="INACTIVE DIPEPTIDYL PEPTIDASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="6">
        <location>
            <begin position="140"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="5">
        <location>
            <begin position="588"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013924035.1"/>
        </source>
    </evidence>
    <sequence length="804" mass="91897" checksum="0E0D6575B1ED1603" modified="2020-10-07" version="1">MTAMKEQQQQAQSNPGNRGGQAPSQDQELGSNSPPQRNWKGIAIALLVILVVCSLITMSVILLTPDELSNSSETRLSLEDLFRKEFVVHNPDAKWINDTEVVYKNRNGHVIKLNVETNTTTLLLENATFVTFKASRYSVSPDLRYVLLAYDIKQVFHYSYTASYVIYNIQTREVWELNPPEVEDSVLQYAAWGVQDQQLIYIFENNIYYQPDVKSSSLRLTSSGKEGIVFNGIADWLYEEELLHSHITHWWSPDGERLAFLMINDSLVPNMLIPWFTGSLYPKGKLYPYPKAGELNPVVKLYVVNLYGPTHTLELMPPDSFKSREYYITMVKWVSNTKTVVRWLNRPQNLSILTVCETTTGACSMKYEFTSEVWLSKQNEEPVFSKDGNTFFMTVPVKQGGRGEFHHIAMFNVQIKNEQFSIRHLTSGNWEVIKILAYDENTQKIYFLSTENSPRGRQLHSVSTTELLNRQCLSCNFMKDRCTYFNAKFSPSIKYFILQCKGPGIPIVSLHSTVNPEKFYILESNSVLKEAISMRKTLRTETRMLHIDDYELPLQLTFPKDFSDRNMYPLLLIVDEVPGSQLVTDKFHIDWDSVLVNSDNVIIAQFDGRGSGFQGLKILQEVHRSLGSVEVKDQIAAVEYLLKQSFIDGNRLSIFGKGYGGYLASMILKSNEKLFKCGAVIAPITDMRLYASAFSERYLGIPSKEENAYQGSSLLHNIHGFMEGNVLIIHGTADTKVHFQHSAELIKHLIKAEVNYTMQIYPDEGHYITSEKSKYHLYSTILNFFSACLKEETPILPQEVEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJW1</accession>
    <name>A0A6I9YJW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transmembrane ascorbate-dependent reductase CYB561</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome b-561</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Cytochrome b561</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106551049</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Transmembrane reductase that uses ascorbate as an electron donor in the cytoplasm and transfers electrons across membranes to reduce monodehydro-L-ascorbate radical in the lumen of secretory vesicles. It is therefore involved the regeneration and homeostasis within secretory vesicles of ascorbate which in turn provides reducing equivalents needed to support the activity of intravesicular enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>monodehydro-L-ascorbate radical(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + L-ascorbate(out)</text>
            <dbReference type="Rhea" id="RHEA:66524"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:59513"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66525"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">Secretory vesicle</location>
            <location evidence="12">Chromaffin granule membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924532.1">
        <property type="nucleotide sequence ID" value="XM_014069057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJW1"/>
    <dbReference type="GeneID" id="106551049"/>
    <dbReference type="KEGG" id="tsr:106551049"/>
    <dbReference type="OrthoDB" id="907479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042584">
        <property type="term" value="C:chromaffin granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000001">
        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF14">
        <property type="entry name" value="TRANSMEMBRANE ASCORBATE-DEPENDENT REDUCTASE CYB561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="20">
        <location>
            <begin position="34"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013924532.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24115" checksum="8CF50FB497346469" modified="2020-10-07" version="1">MRLKLKSHLGKRPKMDETPARPASPIGLTYVVAASQILGLAVITVMAAWIGQYRGGVAWDSSQLLFNVHPLCMVIGMVFLQGDALLVYRVFRNESKKSTKILHGLIHFFALIIALVGLIAVFQHHKNQGFPDMYSLHSWCGIIAFGFYLAQVRDTYNLFVSEGILANTLGLLIIAFGAVVGYILTREEWRRPPLAEELALSMDFKNLTEEGSPTSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKA4</accession>
    <name>A0A6I9YKA4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Neurotrophin receptor-interacting factor homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106551096</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924586.1">
        <property type="nucleotide sequence ID" value="XM_014069111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKA4"/>
    <dbReference type="GeneID" id="106551096"/>
    <dbReference type="KEGG" id="tsr:106551096"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="160"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013924586.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33554" checksum="4CDE41F2A56A49F1" modified="2020-10-07" version="1">MSLEREVEISASGIQRVIVDPDMKPEIKMEKQDLKPERHAHLAGHTKVESGGLYPSLQHIKQEDNESSEPQQWEAQWCNFLGPASSLPLGQGLSELPEAVLQHSPKAFPSILEGAEAVSEWAKAEKVPKVTLDSRDFRIPSGGSEEPTENPEAGNSECQRQHFRQFSYREAEGPWETYCQLRKLCHRWLMPERHTKEEMLELVILEQFLAILPLEMLSWIQERDSKSCFHAVALAESFLLRQREKPDQQVRVTLLLEMLGGWGTPPTEVGCWVKDGILAVLDSQVHLVTVQNCNGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLR1</accession>
    <name>A0A6I9YLR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">LanC-like protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Testis-specific adriamycin sensitivity protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LANCL2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Necessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). PIP-binding enhances membrane association.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the LanC-like protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925071.1">
        <property type="nucleotide sequence ID" value="XM_014069596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLR1"/>
    <dbReference type="GeneID" id="106551486"/>
    <dbReference type="KEGG" id="tsr:106551486"/>
    <dbReference type="CTD" id="55915"/>
    <dbReference type="OrthoDB" id="10257263at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031179">
        <property type="term" value="P:peptide modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04794">
        <property type="entry name" value="euk_LANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000022">
        <property type="entry name" value="LanC like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007822">
        <property type="entry name" value="LANC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020464">
        <property type="entry name" value="LanC-like_prot_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736">
        <property type="entry name" value="LANC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736:SF11">
        <property type="entry name" value="LANC-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05147">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01951">
        <property type="entry name" value="LANCEUKARYTE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01950">
        <property type="entry name" value="LANCSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01260">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158745">
        <property type="entry name" value="LanC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR607822-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013925071.1"/>
        </source>
    </evidence>
    <sequence length="462" mass="52621" checksum="AD79BAE4F9E4DA81" modified="2020-10-07" version="1">MGEGMSKRLKVQLGSEAEMEERAFNNPYPDYQPHGSTASGSSTLPTPSDLEGNRCSSASPQRDDSFPFESDGQITSYFNKKITTKIKDLLQQMEEGLKTADPHDCSAYTGWTGIALLYLQLYRVTKEPIHLQRSLDYVKRILRNLNGRRVTFLCGDAGPLAVGAVVYHKMKNDSESKECVTKLLQLQRIVLASDSDLPDELLYGRSGYLYALLYLNTEIDPETVPQSVIKEVVESIIESGENFSREERKTERCPLLYQWHRKQYVGAAHGVAGIYYMLMQPAANVERETLMELLKPSIDYVRHKKFRSGNYPSSLSNETDRLVHWCHGAPGAIHMLMQAYKIFKEDKYLKDAMECSDIIWQRGLLRKGYGICHGTAGNGYSFLSLYHLTQDKKYLYRACKFAEWCLDYGAHGCRIPDRPYSLFEGMAGTIHFLSDILVPEKSRFPAFELSPQMKENKEERNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLZ6</accession>
    <name>A0A6I9YLZ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase 1, mitochondrial</fullName>
            <ecNumber evidence="2">1.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106551782</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.3" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013925413.1">
        <property type="nucleotide sequence ID" value="XM_014069938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLZ6"/>
    <dbReference type="GeneID" id="106551782"/>
    <dbReference type="KEGG" id="tsr:106551782"/>
    <dbReference type="OrthoDB" id="6718861at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000007">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="8">
        <location>
            <begin position="269"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013925413.1"/>
        </source>
    </evidence>
    <sequence length="663" mass="74361" checksum="DEF57EC07261E2D5" modified="2020-10-07" version="1">MWWWLKAWGRLPETAFGRDYSWQPRAGLPRSTGLQKYQASFPWEQDPSVRKPLVPCRWNHAKEAEGELGFLGIVESYFDRAARVVENELVKRLRTPKDENERVLRVRGVLDAIRSCGHVLEVAFPVRKDSGCWELIKGCRAQHSQHHMPCKGGFRYSKSVNSDEVKALAALMTYKCAVVDVPFGGSKAGVAIEPKNYSKNELEQITRKFTLELAKKGFIGPGIDVAAPDINTGEREMSWIADTYACTLGHKDINSYACVTGKPISQGGIHGRISATGRGILYGIENYINNSTYMDLIGLKTGFPGKTFVLQGFGNVGFHSMRYLQRNGACCICVEEVDGAICNTDGIDSNELQHYKQQIMTVATKYNLGLDQRTAAYICAIEKIFKTYIEANSKIITPSQQTLQDTPILYSSANCCGHKASADFSDLDYNWDSELSQRLHYPSSELKSCNLYIVELQIVGDFYVPLLRCVPVQGQDNDFITVTYDRPHYVPVSKHHIDTVTIEIKTDQDKNVHFYHGKIIAEGANGPTTPDAHDIFLQRKILVLPDLYINAGGVTVSFFEWLKNLNHISYGRLTFKYERDSNYHLLMSVQQSLERKFGKSCGSIPIIPTPEFQARIAGASEKDIVHSGLAFTMERSAGGLVSDNEMAEEEILGMGEMVYYLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNA4</accession>
    <name>A0A6I9YNA4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C7orf57-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925747.1">
        <property type="nucleotide sequence ID" value="XM_014070272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNA4"/>
    <dbReference type="GeneID" id="106552082"/>
    <dbReference type="KEGG" id="tsr:106552082"/>
    <dbReference type="OrthoDB" id="10012494at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040247">
        <property type="entry name" value="DUF5524"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31097:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31097">
        <property type="entry name" value="SI:DKEY-276J7.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17662">
        <property type="entry name" value="DUF5524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013925747.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="22866" checksum="196F2C6F483B0807" modified="2020-10-07" version="1">MRLPPRILGMRNTNKESHGPTDRYAPCDWYYHIPLKRSEEQVSSEVAQQPTSQIPGLGDFGDTHNDITFGGRRKWIKDTDSEYVKLAKQGGRPDLLRHLPPTPQKVSLGPYGAPEWYSHHSKKAANDSKSHSPAIPDYMIHEEFKSDQPASYEPKKGPFDFDMKSVWQRDAEDKENKEKRENNCFEYEIVDMEVFQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ04</accession>
    <name>A0A6I9YQ04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein 646-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106552580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926372.1">
        <property type="nucleotide sequence ID" value="XM_014070897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ04"/>
    <dbReference type="GeneID" id="106552580"/>
    <dbReference type="KEGG" id="tsr:106552580"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000729">
        <property type="entry name" value="Zinc finger protein 646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001345">
        <property type="entry name" value="zinc finger protein 646"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002353">
        <property type="entry name" value="Zinc finger protein 646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF235">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="567"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="595"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="679"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="720"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="747"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="775"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="806"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="608"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="628"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="657"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013926372.1"/>
        </source>
    </evidence>
    <sequence length="846" mass="95794" checksum="A9F235A20EC4D52F" modified="2020-10-07" version="1">MPMILSLAEQLRDSCSVLEKDATDNSWQSQSQASSPLPADSESLPYLCEQCGLGFDQAKTLIDHRQTHQAGIFQCSVCPKEYPTLLALKHHFHGHAKATASSQDNPKDHAEEQPFLCSLCGMIFPSEETLQHHHSLLHEEDESQGDNPDVLKTEAEEQLDDGGDPEEDQLLSHICGYCGQTFDDMSSLEEHSNGHLEEKAAALADTSIQLRRALRIEDDPPPLPPPLVETLPLADDLLDSRPYACGQCGKTYRHGGSLVNHKKIHLIGDYQCSVCCRQYRNLSAYRNHLRNHPRCKLNSSVPQLRHPVPAVERGLPCVYERLKDEPCGASQNGEAPPSNSFLAPPAGKVKVEQGGELSLGLLEGREAQECNVPGEGNEKEEAHQAIEEMETIKKQGEGAGEEKVSQAEEEDSLSERPFQCGVCGRSYKHAGSLINHRQSHKTGLFHCSICQKQFYNLMALKNHNRTHFETKRYRCTECPKAFRLQKQLASHQHMHWDRRPEPAFPSVQRPLRAKRAGKAETFPLLRAPAKQLPNPEERPYRCGQCGRSYRHAGSLLNHQKSHKVGHFTCALCSKAYPNLMSLKNHQRIHYEVKRHQCPDCGKAFKWQRQLQRHQRRPHPCNKVMPGQEPERTKADCEKDQGNNPGEAPAGTLQSQPRPKRFRNHASQKNPSRVRGKKRLECSDCGKAYRASRDLMQHLRRHQAEERGDSPKGKLLEDQPYKCHSCERTYRHPGSLLNHKKAHATGLYHCPTCQRDFYNLLALKNHLHIHTDKRRHRCPHCGKAFRTAKQLTAHTKVHGRSKEEKTFSCPVCSKRFFHHLTFQQHQLLHTKVDGHHALEGSITGEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQQ7</accession>
    <name>A0A6I9YQQ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">V-type proton ATPase subunit S1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ATP6AP1L</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vacuolar ATPase subunit S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926627.1">
        <property type="nucleotide sequence ID" value="XM_014071152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQQ7"/>
    <dbReference type="GeneID" id="106552797"/>
    <dbReference type="KEGG" id="tsr:106552797"/>
    <dbReference type="CTD" id="92270"/>
    <dbReference type="OrthoDB" id="9442153at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033176">
        <property type="term" value="C:proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030641">
        <property type="term" value="P:regulation of cellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.110:FF:000003">
        <property type="entry name" value="ATPase H+ transporting accessory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008388">
        <property type="entry name" value="Ac45_acc_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046755">
        <property type="entry name" value="VAS1_LD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471:SF3">
        <property type="entry name" value="ATPASE, H+ TRANSPORTING, LYSOSOMAL ACCESSORY PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05827">
        <property type="entry name" value="VAS1_LD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1 luminal" evidence="7">
        <location>
            <begin position="180"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="8">
        <location>
            <begin position="343"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926627.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="48261" checksum="68CFE6246F69D4C6" modified="2020-10-07" version="1">MAIGVPLGDLNTNLSKFDSTHYTQFNTVRCLLNIIPSGEPGQLYSVVSFIVLITEPGSSVKEDTDTVNLARSDRNVEGCSTQRGFKFHVLIAGGEVLWSFYTHFDLLVVPFEWLFVFSDQDPLQRNGRRYDGSVKPKINLQQVAITQIGNYNVSTKENLEKESLFHSHYSPLNVTVNGSPCILFWARRIMIKFQNHTQLDLTDKTFGVHASVDIADSNCSEENAMLSLNFGDIDNLKGFVIRFILTNSYYKLSIQNWFTLHRIQLIYNQSVQATFNATRIHAPAIYSYHCEHVSNLQRYDALLTPSSANDEAKHWEVTFIDFQIQGFNIEDGQFAYAKDCASLFPPAILMGLVMSLILLLVLAYALHMLMHLKSIDRHYECKASSAYYPQMKDIDTADEKEPLRIDGHETYELRSQQYSKIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRY1</accession>
    <name>A0A6I9YRY1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM69A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106553355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927303.1">
        <property type="nucleotide sequence ID" value="XM_014071828.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106553355"/>
    <dbReference type="KEGG" id="tsr:106553355"/>
    <dbReference type="OrthoDB" id="8860232at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029244">
        <property type="entry name" value="FAM69_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21093:SF4">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21093">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14875">
        <property type="entry name" value="PIP49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01299">
        <property type="entry name" value="PIP49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="FAM69 N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927303.1"/>
        </source>
    </evidence>
    <sequence length="163" mass="18930" checksum="563066F87EBB595C" modified="2020-10-07" version="1">MARRVSAGAWLRKPYYGQVRFSYMRMKYLFFSWLGVFIGSWIIYVQYSSYTELCRGHDCKKTICDKYKSGVIDGSACSSLCTRQTLYFRKCLSTQPNNQMYLGEWGNLQGVIKCQMEEIAHIDLRTELEPRKQTTLFDKPTRGTTVQKFKEMVYXGVSSPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS37</accession>
    <name>A0A6I9YS37_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">28S rRNA (cytosine-C(5))-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">NOL1/NOP2/Sun domain family member 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NSUN5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926435.1">
        <property type="nucleotide sequence ID" value="XM_014070960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS37"/>
    <dbReference type="GeneID" id="106552636"/>
    <dbReference type="KEGG" id="tsr:106552636"/>
    <dbReference type="CTD" id="55695"/>
    <dbReference type="OrthoDB" id="435282at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000004">
        <property type="entry name" value="probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000139">
        <property type="entry name" value="probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049561">
        <property type="entry name" value="NSUN5_7_fdxn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21148">
        <property type="entry name" value="NSUN5_fdxn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="14">
        <location>
            <begin position="113"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="220"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926435.1"/>
        </source>
    </evidence>
    <sequence length="451" mass="49992" checksum="E46101EE88807579" modified="2020-10-07" version="1">MILPVTLSQLQGKVALTLSNTRLLYALVSETRRYSSVLDSIIDAAGLFRAEKKLQPHLAKVLVYDLLIGKGLHGGGRGKALVLKHHARLQAELARLKVQKKVSRNEDLLPPAGDSDKALQMPRYVRVNLLKTCVDDVVDYFKRQGYSYQGKAARMEEAILSRKKFLLDLHLPDLLIFPPQSDLHENQLYQAGHIILQDKASCLPAFVLNPTPGSQVIDACAAPGNKTSQLAAIMKNKGRIFAFDLDAKRLATMSTMLVRAGVACHELAHQDFLATDPTDSKYSKVRYILLDPSCSGSGMVNRLAEEESTSLPNRLQALAGFQRKMLAHALQFPALQRLVYSTCSVHKEENEDVVQDVLEQEGSAFRLVEVLPSWTPRGLPVFPEAKCFLRASPEETLTNGFFVAVLERKHKAYAASSAVLLSEADSKLETNPNPATRKRKKKKTQTAFPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS85</accession>
    <name>A0A6I9YS85_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM13B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106553435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927408.1">
        <property type="nucleotide sequence ID" value="XM_014071933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS85"/>
    <dbReference type="GeneID" id="106553435"/>
    <dbReference type="KEGG" id="tsr:106553435"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039102">
        <property type="entry name" value="FAM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059029">
        <property type="entry name" value="FAM13A_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904">
        <property type="entry name" value="FAM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904:SF16">
        <property type="entry name" value="PROTEIN FAM13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26116">
        <property type="entry name" value="FAM13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM13A-like" evidence="2">
        <location>
            <begin position="75"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927408.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="12878" checksum="365347214020AC9B" modified="2020-10-07" version="1">MLQPIIEGETAHFFEEIKEEEEDGDISSELTDILKSSAQIHPIATSPVENSESDVEESQEKLTRELRLSSIRAASMPELLEQLWKARGEKKKLRKTLREFEEGFYQQNGRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTS7</accession>
    <name>A0A6I9YTS7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ciliogenesis and planar polarity effector 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">REM2- and Rab-like small GTPase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RSG1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927968.1">
        <property type="nucleotide sequence ID" value="XM_014072493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTS7"/>
    <dbReference type="GeneID" id="106553906"/>
    <dbReference type="KEGG" id="tsr:106553906"/>
    <dbReference type="CTD" id="79363"/>
    <dbReference type="OrthoDB" id="10266641at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039677">
        <property type="entry name" value="RSG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14983">
        <property type="entry name" value="CILIOGENESIS AND PLANAR POLARITY EFFECTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14983:SF1">
        <property type="entry name" value="CILIOGENESIS AND PLANAR POLARITY EFFECTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013927968.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="28056" checksum="3DC8EB8A48BFCCBF" modified="2020-10-07" version="1">MIVRPGSVVDPDWHSTEEGRAYSGALLRRAQRRSFGLIERPVVAPQAAADVAGYKVFVSGKSGVGKTALVAKLAGLEVPLVHHETTGIQTSLVYWPAKLRESGKVLFFKFSFWDCGEAMLRKFDHILPACKEKADAVLFLFSFTDRASFLDLPHQISRVTDGTRHMVRMVVGTKFDQFAHTDVTESDMASFSHTWGLPVLRTKSVGERHSDGYAGLAEVAHLLNRLAEHLWRQDQVAAGLVSGEESPTIPQALAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVL2</accession>
    <name>A0A6I9YVL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PSMD5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with PSMC1, PSMC2, PSMD1 and PSMD6. Part of transient complex containing PSMD5, PSMC2, PSMC1 and PSMD2 formed during the assembly of the 26S proteasome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S5B/HSM3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928041.1">
        <property type="nucleotide sequence ID" value="XM_014072566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVL2"/>
    <dbReference type="GeneID" id="106553970"/>
    <dbReference type="KEGG" id="tsr:106553970"/>
    <dbReference type="CTD" id="5711"/>
    <dbReference type="OrthoDB" id="10250600at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000208">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019538">
        <property type="entry name" value="PSMD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13554:SF10">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13554">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10508">
        <property type="entry name" value="Proteasom_PSMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928041.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="56130" checksum="F9290B0E4F205D5D" modified="2020-10-07" version="1">MAAAEVEAALALLSRVAALEEPLEELRALRLAVQALAPAALRERGPRYPLAGLFVLMARGDTEQISLCVSILERFLQVMDPLDVIQNFGEELQKGLFHPDDSVKILTISQVGRVVEDAVAVREILNTPELLRQIVYCIAAEKIAVAKEAIKSLSRIAQSPEGLEVLLAGNLLIDLKNVMAKSDIIRYRVYELVINISSTSVDSLNYCVNSGLLSEMIEELTGNDVLVRVTCIEMVTSLAQTHHGRQYLSQQGIIDKISNIITGADSDPFSGFYLPGFVKFFGNLAIVDSPQHICEQYPAFVGKVFSMAEGQDPTLFGVAVDTLAILGSNVEGKLVLQKTGSRFHRVLNRIGHQIKNAPTETRIRCLDAISSLLFLQPEQQTEDLLRMTELWFSSLSNQPLELFKSISTQPFPDLHCAALRVFTAIANQPWAQQEMLASPGFVEYMVDRSVEPDKASKEAKYELVKALVSSKTAAEIFGNQHYLRLKTYMREGPYYIKAVSTIAVEGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVT3</accession>
    <name>A0A6I9YVT3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleolar and coiled-body phosphoprotein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928121.1">
        <property type="nucleotide sequence ID" value="XM_014072646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVT3"/>
    <dbReference type="GeneID" id="106554047"/>
    <dbReference type="KEGG" id="tsr:106554047"/>
    <dbReference type="OrthoDB" id="5599646at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039191">
        <property type="entry name" value="Nopp140-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216:SF1">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="416"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928121.1"/>
        </source>
    </evidence>
    <sequence length="491" mass="53018" checksum="5527CF2C11196E04" modified="2020-10-07" version="1">MSKTINGKAESSSSSSSDDTSSSEDSSSEENQPVKGKLKKLPHCKNNIAQPLPIKTKQGKKSNSETSCDNSEHERLASKPKRGEYSAVPPPLAIESKKGKAPLIKKKTFKKNGENNEKKEQVFTKSPSTEGSVIKKAFAPKSLPVSIQKGNTSSESDSDSSSEEEKKVLVKCSAKAVSANVAVKSVPFKKVSSSSDTSDSDSSDSIPEKSAVKTGIKKVKKPLGVLAKKVESSLENKKVHKIKGTKAVKTTPKSSTPISKPKSSESSSSSSSSSSSSEEETQPSQKAAGKLSVGSSLIVSKQTPAEIRTSTENSTDEFKISEKTERKRGQNNCKTAKKVLKTSANLVGKTSALKTPTSEVVNSANSSSSDEEATKEGLSKKKRKRKEEQELETPDIKKMKATSPHTFPKSKRKSSPFRRIRTEEIEIDVRVANNSFDAKRGAIGDWGEKANDVLKFTKGKSFRHEKTKKKRGSYCGGTISTQINSIKFESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX42</accession>
    <name>A0A6I9YX42_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptor 171</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">GPR171</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928525.1">
        <property type="nucleotide sequence ID" value="XM_014073050.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928526.1">
        <property type="nucleotide sequence ID" value="XM_014073051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX42"/>
    <dbReference type="GeneID" id="106554402"/>
    <dbReference type="KEGG" id="tsr:106554402"/>
    <dbReference type="CTD" id="29909"/>
    <dbReference type="OrthoDB" id="9935079at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045028">
        <property type="term" value="F:G protein-coupled purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15167">
        <property type="entry name" value="7tmA_GPR171"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000206">
        <property type="entry name" value="Probable G-protein coupled receptor 171"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048077">
        <property type="entry name" value="GPR171"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233:SF4">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 171"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
        <property type="entry name" value="P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
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    <sequence length="336" mass="38610" checksum="F0F6EE5F0F07B7F0" modified="2020-10-07" version="1">MASTNSSECNIYSQMQPFTYIYYLIFLIGFIGSCFALWTFLKTKKHRKCINVYLINLLTADFLLTLALPVKIIVDLGIAPWKLKIFHCQVTACLIYINMYLSIIFLGFLSMDRCLQLTKGYMIYRIQEPGFAKMISAVVWVMVLFIMVPNMIIPITEVEESPNVGCIDFKTKLGRDWHVFTNFICTAIFLYFSAMVLISNFLVIRLLFQNKGTEGYLNVKKALVNILLVTASYIICFLPYHIVRIPYTLSQSKALITDCFLRESLYKAKESTLLFGVSNLCFDPILYFYFSKTFRLTVTKSFASFKGRHVSLDDQEMHGEKTPHFQVTAETAILSA</sequence>
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<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXF3</accession>
    <name>A0A6I9YXF3_9SAUR</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the adenomatous polyposis coli (APC) family.</text>
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        </source>
    </evidence>
    <sequence length="2332" mass="258234" checksum="CA07616C1835F791" modified="2020-10-07" version="1">MQMDLIRQQLEFEAQHIRTLMEERFGTTDEMVQRAQIRASRLEQIDKELMEAQDKVQPSEQPPSGKLPSTEGDGRLDIATNSEEAKDQLGSSKVEVVFWLLSMLATRDKEDMSRTLLAMSSSQESCLAMRKSGCLPLLIQILHDSDREPSAPNSPGAAGKDCRMRANAALHNIIFSQPDEGQAKKEMRVLHVLEQIRSYSETCWDWLRMQKEGAKIPEGGAAPVPIEPQICQATCAIMKLSFDEEYRRAMNELGGLQAIAELLQVDYEMHKMTNDPLNLALRRYTGMALTNLTFGDVVNKATLCSRQRCMQAIVDQLASDSEELHQVVSSILRNLSWRADMNSKKVLREVGSVSALMQCALRASKESTLKSVLSALWNLSAHSTENKTAICMVEGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLIATREDYRQTLRDHNCLQTLLQHLKSHSLTIVSNACGTLWNLSARSPKDQELLWDLGAVSMLRNLVHSKHKMIAMGSTAALRNLLANRPLKYKDAMVISPGSCMPSLYMRKQKALEAELDAQHLAETFDTMEKQTMKKQSVAKKPMRHMDSLAKDYASDSGCFDDDEVPNVSSSTETGNASVLSMFLNSSFLQSQTLSRTVSQKRGPEPEKDPSGKSTEAKKASPLLVSQDNDVSVVAEKLANKITTTVAKIDKLVEDITTLHTSSDDSFSLSSEDHCLDWQYGLDEVHEARAQSCSPCRLSDTSGIMKRESLSRAHTLLRLKNAYTSLSNDSLNSGSTSDGYCPKEHMRPCTRTTFLDFKDELHRYQKRPSRLDLKNILLNRPEKMELPEKRQPESNVMAEKPEKETLLEKAKVTRLPPNPDVAEKETECKKKEGGRQLDPDPKVHTIKLSPSYQHVPFLENNTIASGTTGVLGTSSYYPSLSMKTTDHLNKVPEKLTAAEPAKQERLQKYSMEEMPICFSRCSSLSSISSADGQSHSENEMDTDSSLEIIEVEEVEEAQKNNQEKKAKQEVEPLNSSSQPIAIPFPNKEKMFLRGASPSRHDDLTPSSSSENYIQETPLVMSRCSSVSSLGSFESPSIASSIQSEPCSEMISGTVSPSELPDSPGQTMPPSRSKTPLFELNSQPEKEASPFNIQWENNVKKFMDITDFKERFQMPQDLDSMVYFTVEKPNENFSCASSLSALPLHEHYIQKDVELKLIPPFPERSGLNFMTHEKRDDERAIEGQRKLECLELTSDEDIEILRECINSAMPPRFRKIRTSLVSGLPGQVLNPQSKKSMQLPVYMLVPANSHLSIPKHRWATVTRPNKDYLGNEDSFTDSAEGTPVNFSSAASLSDETLQYPMKDEGDSRHCSGKRKEVTGVPMGGLQREIRQLEQSSCRIGRITSSHSTPTKMASSSKYKTGSNHTSPLRATRAQSAESRRGNIPLRNLELTFLRQGSPRASSEGNRSKKKEEPRENSSHDSDGAFPSFCHTTPTEEAVYCFYENDSDDLVELGMKNSPKRKPNLAGKGQNKECWAKKEPELNSSQQLHAKNKMAPHLRSNLIADETPPCYSLSSSLSSLSDLELYGTGERMQMSKIRANRQLTMNRRQGTRAAVSKGRDSSASSLSYNSDNDLLQKGLGSSGPKRRRHSAKRRNVEKEQKGLKSKEWKPEGFPDEIASEKGSDLDSVDWKAIQEGANSIVTWLHQATSSIGKETSSESDSILSFMSGLSASSALQLSLDRRDKTRGRKGPTGGVEKKNLPKSFQHGKNLNETRGAGSRNKTEKNVGQQKPLSNIPVVFRGRTVIYMPDAAKESPVQSSRSTPKKTGGPTKQEIPEKTLNLNQPRSRSLHRPGKISEMVELMLPKRSATPPARMNRAPSSGSSRTSTPSQPNPKKLTSPSQQPGRQTPSREAGKTSGSTTPVGPAPKPVQKSPVSKQHKTQKSPVRIPFMQKPVKKTLPMRNTMPTLEERGGNCSSELRAKVNGKGILQANRLNLMRMSSARSLSSDSHQSGFLRQLTFIKESPSLRMRQRSEVSSSEPLSSLSQGASPRRGRLGLPTVFLCSSRCDDLKVDKPTGSSQRPVISRTPLLSQSPSAGVPRRPPRRTSSESPSRLPLKSNNVPPEPFKRYSSSPQINIIKRAASPASTPSDSPEPSSGRKKNEEVSKLPVIPPAEKGTENQQRQRPQQQPQPQQPPPQPQPQQPQPPQQPAMVKGTWKRIRDEDIPHILKSTLPSTALPLVSAWREEESDQTPGVLQPKTSDAVVQTEDFSTTKTNSSTSPTLESQKAVQIPPDSYEGDGFALAKATTASISFTHEGSVSSLGNFPSSRHSSPSRAARVTPFNYTPSPMVEPTVKEKQVEKTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY75</accession>
    <name>A0A6I9YY75_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Olfactory receptor 2T27-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106555018</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929257.1">
        <property type="nucleotide sequence ID" value="XM_014073782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY75"/>
    <dbReference type="GeneID" id="106555018"/>
    <dbReference type="KEGG" id="tsr:106555018"/>
    <dbReference type="OrthoDB" id="9828669at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013929257.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="23398" checksum="A67C373B98331E03" modified="2020-10-07" version="1">MGGAECLLLTVMPYDRYMAICKPLHYPVLMSRRVCVAMSGGVWFGAILHSIVHIFCVFQLPFCKSNRVNQFFCTVQALLKLSCSETSTYENWLFLTGSILLLGPFSIILASYISILLTVLGMQSVEGRQKAFHTCLSHLCVVGLFYGAASFKYMRPRSYRTPEQDKVASLFCDIVTPMLNPLIYSLRNRDVLLELRKHIKKCKLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZN8</accession>
    <name>A0A6I9YZN8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">USP36</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013930183.1">
        <property type="nucleotide sequence ID" value="XM_014074708.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013930184.1">
        <property type="nucleotide sequence ID" value="XM_014074709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZN8"/>
    <dbReference type="GeneID" id="106555797"/>
    <dbReference type="KEGG" id="tsr:106555797"/>
    <dbReference type="CTD" id="57602"/>
    <dbReference type="OrthoDB" id="420187at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000016">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF653">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="120"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="482"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="534"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="583"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="792"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="495"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="607"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="813"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="861"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="884"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="933"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013930184.1"/>
        </source>
    </evidence>
    <sequence length="1116" mass="122920" checksum="1077D6C1D4DD85F5" modified="2020-10-07" version="1">MPIVDKLKEALKPGRKDSADDVELGKLLAASAKKILLQKIEFEPASKNFSYQLETLKSKYVLLNPRTENCSRHKSSEEGPIRKQGNEHVSGSDGVPAPQKVLFPLERLSLKWERVYRVGAGLHNLGNTCFLNSTLQCLTYTPPLANYLLSKEHSRNCHQGGFCMMCIMQNHMIQAFANSGNAIKPVSFIRDLKKIARHFRFGSQEDAHEFLRYTIDAMQKACLNGYTNHPPACLFRLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDVALEIRQAANIVRALELFVKADVLSGENAYMCAKCKKKVPASKRFTIHRASNVLTISLKRFANFSGGKITKDVGYPEFLNIRPYMSQSSGDAVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNIKVVLNQQAYVLFYLRIPSTKKNPEGLVSKSNCSMPSRTNISSDHLKKSATNGTLASPLINKRHNVIQVKKASDPEESGVPVSRNTFVSNNKTQNGILPPPLPKLIPAVGFSSPKFSSKPAFLDDLSKRSKKQASSSRKTSQDFGQADSAKLELAKQSFWGAKNLVASLQLSGSSGGTPEAKAHEEGTELPSKDSSLTSSSSSSSSSSSSPAHKPIKGLSQATVGADGYSSSQESDCVSGKYTSEDTKFAKMKSPLLTNLANLELGSTMSPPPAKKLALSARKGSNTLRKVSGNEHQAPPHFPPAATYTTQFVCAAASIQARVVSPASKSSAVPQTANSPFANSMPSSSFKSCCLSAATLLPSPVVPTSAPHLCYLDNSKEPSVVSSKKKKRKHSSPVEEQDGHGSRNMKDFNGERTWLSSPPCKRRQFEGNGSIVISPAQEANLGKTKKGEEEKRAGGDRAIATGQSSSIQKKRKKKKRLQQREEDCSSLLSPVNCCSPPPEVSSCRTKKKKKKKKQLLSPSSDKSTSSGESEHHKDKQRKILHSHVAEVCSPQEELNENGLSVARVSPPALAWGNQFRHRDEFSLNVLSPKKEIMQHECGPVDVVQELLKDSLDKAYGKQVLTWNGEASAISQDAMHDARWSQKQSILDDWDEEFDGGKVKKIKKFKRERRRNYNVFQKLQSRRNFWAVTHPAKVASLSYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0A7</accession>
    <name>A0A6I9Z0A7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">HepA-related protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Sucrose nonfermenting protein 2-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SMARCAL1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929526.1">
        <property type="nucleotide sequence ID" value="XM_014074051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0A7"/>
    <dbReference type="GeneID" id="106555253"/>
    <dbReference type="KEGG" id="tsr:106555253"/>
    <dbReference type="CTD" id="50485"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18010">
        <property type="entry name" value="DEXHc_HARP_SMARCAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001036">
        <property type="entry name" value="SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000026">
        <property type="entry name" value="SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010003">
        <property type="entry name" value="HARP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF6">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07443">
        <property type="entry name" value="HARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51467">
        <property type="entry name" value="HARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HARP" evidence="14">
        <location>
            <begin position="272"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="HARP" evidence="14">
        <location>
            <begin position="376"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="493"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="764"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="66"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013929526.1"/>
        </source>
    </evidence>
    <sequence length="987" mass="110673" checksum="533A9A9BD6E11F73" modified="2020-10-07" version="1">MLPRLTEEQKRKIEENRQKALERRAKRLAAQQGGVAMKLSDRQNLDSCQGNLQRPLKKQSYDTILGNQHLQGPGSHLMQRSPRICSDHCSSNQQATVSHGGQQKDDSMDSSPNLYRQLDSTKKPSLAMQSSLSNSHCHSPESSCRQGQIIQYNSDPLQLPTCGAAGQNTGDQHQTILAANMNLHPVQSQNKSVGKQGLLKQATWLDAGGTTARDCPMQSGTRNLDCAKKKGSNALQFYGTKPLLASVKGGREQSSTVCRVPGNVPEMSEQKNHRILKGKCINHSEGRFRVEIGYNTELIEIFRSLPSKKYDPETRMWNFSLEDYGELMEKVEQFPSVSLTSLEGMEKMTSGGESSSASQNSQLRSLLKTCTGWQKPSATLKGRCILISYSRFEVDIGYAANIVGVFKQVNSRIYDMKTRKWNFLLEDYLRLMELVKSLPDVQLKPLPKPVIMAFVTQFEKSLSKATDPPEADLVGVDPKLVTSLLPFQREGVDFAIYREGRLLLADDMGLGKTIQAICIAAYYRKEWPLLVVAPSSVRYTWAEAFHRWLPSLNPDTIRVIATGKDQVSAKLINIVSFDLLSKIKKQLKNPFQVVIVDESHFLKNSKTARCQTAMPLLKAAKRVILLSGTPAMSRPAELYTQIAMVQPTFFSQFHDFGVRYCDAKQRPWGWDYSGSSNLGELKLLLEETIMIRRLKSEVLSQLPAKQRKMIVITPEGIDTRTKAELTAAAKEMAKGFKSKQEQKEALLIFYNKTSEAKIHCILEYILDLLESGRQKFLVFAHHKLILDAISEELKKKNVGFIRIDGSTPSAERQSLSQEFQFSKKLSVAILSLTAANMGLTLSSADLVVFTELFWNPGVLMQAEDRAHRIGQTSSVDIHYLVAKGTADDYLWPLIEKKIRVLGEAGLSEAKFSETAETTNYYICKEDTKQQTMLDLFQRTFSEDIDDMDEALLGEIVETCEDSGQENPLNNQAGHFFSASPCKKKRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0M3</accession>
    <name>A0A6I9Z0M3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IQ motif and SEC7 domain-containing protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930543.1">
        <property type="nucleotide sequence ID" value="XM_014075068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0M3"/>
    <dbReference type="GeneID" id="106556098"/>
    <dbReference type="KEGG" id="tsr:106556098"/>
    <dbReference type="OrthoDB" id="9049755at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930543.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="31490" checksum="28346EF7995C9064" modified="2020-10-07" version="1">MESLLENPVRAVLYLKELTAIVQNQQSLIHTQRQRIDELEKRLDELSTENRSLREHYALPAEAPAPPPLQPRVPPAASLPCRAPPQGQQQPAAAEQEEFQHHQQLPALQQPPPRHPPPPPPSSSKQAQAGPSGTRTPISHQHPSLQHHQHHHEDSREKSCCPALLPHKSPPTLGKGGLVRRLENETVLHQFCCPAADAEQKPSSAESSSSDDPCGSASKKTGCDVEEPKESGRGSGQEGRSDTLKLKAQHEERQEAQGAGSSLLVQKASLHHTGSPVRGQRSKGAATCSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0U5</accession>
    <name>A0A6I9Z0U5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Folliculin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">FLCN</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Interacts (via C-terminus) with FNIP1 or FNIP2 (via C-terminus). Component of the lysosomal folliculin complex (LFC), composed of FLCN, FNIP1 (or FNIP2), RagA/RRAGA or RagB/RRAGB GDP-bound, RagC/RRAGC or RagD/RRAGD GTP-bound, and Ragulator. Interaction with FNIP1 or FNIP2 mediates indirect interaction with the PRKAA1, PRKAB1 and PRKAG1 subunits of 5'-AMP-activated protein kinase (AMPK). Interacts with HSP90AA1 in the presence of FNIP1. Interacts with HSP70, STUB1, CDC37, AHSA1, CCT2, STIP1, PTGES3 and PPP5C. Interacts with GABARAP; interaction takes place in the presence of FNIP1 and/or FNIP2. Interacts with RILP; the interaction is direct and promotes association between RILP and RAB34. Interacts with KIF3A and KIF3B. Interacts with lactate dehydrogenase LDHA, but not LDHB; the interaction is direct, may preferentially bind LDHA dimers rather than tetramers, and regulates LDHA activity, acting as an uncompetitive inhibitor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the folliculin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930202.1">
        <property type="nucleotide sequence ID" value="XM_014074727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0U5"/>
    <dbReference type="GeneID" id="106555812"/>
    <dbReference type="KEGG" id="tsr:106555812"/>
    <dbReference type="CTD" id="201163"/>
    <dbReference type="OrthoDB" id="5599713at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030511">
        <property type="term" value="P:positive regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1730:FF:000001">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12430:FF:000001">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1730">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044886">
        <property type="entry name" value="FLCN_DENN_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032035">
        <property type="entry name" value="Folliculin_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16692">
        <property type="entry name" value="Folliculin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="20">
        <location>
            <begin position="86"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013930202.1"/>
        </source>
    </evidence>
    <sequence length="580" mass="64809" checksum="1B05CE0DCBD73724" modified="2020-10-07" version="1">MNAIVVLCHFCELHGPRTLFCTEVLHAPLPQGPPGGDSPGQSELAEDEEGGIQMSSRIRSHSPAEGASVDSSSPGPKKSDMCEGCRSLPAGHPGYISHDKETSIKYISHQHPSHPQLFSVVRQACVRSLSCEVCPGREGPIFFGDEQHGFVFSHTFFIKDSLARGFQRWYSIITIMMDRIYLINSWPFLLGKIRGIVDELQGKALKVFEAEQYGCPQRAQRMNTAFTPFLHQRNGNAARSLTSLTNDENLWACLHTSFAWLLKACGSRLTEKLLEGAPTEDTLVQMEKLADLEEESEAWDNSEEEDGKPTSQKDVREGSGQMKNQADFGLLADCSSWNGTSGSQSVFRSLRHLRQVLGSSAFRMLAWHVLMGNQVIWKALDRDLIHSAFDVLRTMLPIGCVRIIPYSDQYEEAYRCNFLGLGPQVKIPPHILSSEFAVLVEVRLATRSSLYPVLFDEDQPLSKYEFVVTSGSPLAADRVGPTILNKIEAALMNQNLSVDVVDQCLICLKEEWMNKVKVLFKFTKVDSRPKEDTQKLLSILGASEEDNVKLLKFWMTGLSKTYKSHLMSTVRSPTSLESRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0V2</accession>
    <name>A0A6I9Z0V2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Major histocompatibility complex class I-related gene protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106555388</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929706.1">
        <property type="nucleotide sequence ID" value="XM_014074231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0V2"/>
    <dbReference type="GeneID" id="106555388"/>
    <dbReference type="KEGG" id="tsr:106555388"/>
    <dbReference type="OrthoDB" id="8936120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07698">
        <property type="entry name" value="IgC1_MHC_I_alpha3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000001">
        <property type="entry name" value="H-2 class I histocompatibility antigen, alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF242">
        <property type="entry name" value="MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-RELATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0490">MHC I</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="241"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929706.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="44889" checksum="82286335F14C219B" modified="2020-10-07" version="1">MDRLDLFLRIPWNFAEGRIPRGSGERRAARLRRASDCRIFFLPALLLLLLRSGCSDGHTHSLLYLYTAVSESIGDLPQFTALGYGGGFLAGSYNSITERAVSRAPWMKKIEEEDPHFWNWMTKRASHSGQRFRKDLENILQENNQSRELHVWQRLYGCELAEDGSESGYDLYAYDGKDFLSFNKATLNWTAANAEAEVTKKKWDADLDRGWRKKIYLEGECFAWLKKYLDYGTETRARKKPPVVTMSSRTEVDGMESLLCRAHGFYPKEIDAFWRRDGEVWLEDTLQGSVAPNSDGTYHYWLSIRIDPKERSHYRCHVEHDGLQEPLDIPMEEAVSTGVYFRRGGGMVIAVISLVVFIGCIVYCVKKKAKDEEEESTTSDDDLEEVKDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0Z9</accession>
    <name>A0A6I9Z0Z9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proline-rich protein 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930683.1">
        <property type="nucleotide sequence ID" value="XM_014075208.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106556218"/>
    <dbReference type="KEGG" id="tsr:106556218"/>
    <dbReference type="OrthoDB" id="8447576at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052466">
        <property type="entry name" value="DNA_MethProtect_Complex"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14709">
        <property type="entry name" value="GLUTAMINE AND SERINE-RICH PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14709:SF1">
        <property type="entry name" value="PROLINE-RICH PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930683.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="16615" checksum="5FBCD79D77904EAC" modified="2020-10-07" version="1">MAYLSKXCTSPCKRLDEELKRNLETLPSFSSDEEDSVSKNQDLQKSISSAISALYDPTDRKDGEPNDVPPVEVKAPSPAPSVEADVQPEQPPPPISPAPSLKEPPPVLEELPPPLLPLPPPPPPPGIPWTMLMSPGNLSTWKRLSASDTEGIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z107</accession>
    <name>A0A6I9Z107_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein-glutamine gamma-glutamyltransferase</fullName>
            <ecNumber evidence="6">2.3.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106555430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N(6)-5-L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:54816"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:14005"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:138370"/>
            <dbReference type="EC" id="2.3.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.13" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013929756.1">
        <property type="nucleotide sequence ID" value="XM_014074281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z107"/>
    <dbReference type="GeneID" id="106555430"/>
    <dbReference type="KEGG" id="tsr:106555430"/>
    <dbReference type="OrthoDB" id="437511at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000090">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000278">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.260.10:FF:000001">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000171">
        <property type="entry name" value="protein-glutamine gamma-glutamyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013808">
        <property type="entry name" value="Transglutaminase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF50">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00927">
        <property type="entry name" value="Transglut_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00868">
        <property type="entry name" value="Transglut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000459">
        <property type="entry name" value="TGM_EBP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
        <property type="entry name" value="Transglutaminase, two C-terminal domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00547">
        <property type="entry name" value="TRANSGLUTAMINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="10">
        <location>
            <begin position="267"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013929756.1"/>
        </source>
    </evidence>
    <sequence length="692" mass="76106" checksum="2E0E4EBC894487CC" modified="2020-10-07" version="1">MAGLVPTQINWLVPSNSQAHNSSCFATTELCVRRGQKFDFKLYFNRAPNAGESLAFVAETGPGPSEAQHTRCGFALSNALTGGTWCAVQTSGDGNCINFSMCSSCRAAIGRYKLSLQVTCGNQVATKFLGKFVLLFNPWCTGDLVFMANEAERQEYVLNENGIIFVGNANYIEARGWYYGQFQENILNICLHLLNLSLYHRQDPAGDASRRSDPRYVGRVVSSMVNGNDGDNGVLEGKWSEDFAHHENPSRWDGSVAILRKWAQGNYSPVQYGQCWVFAGVAGTVLRCLGIPTRLVTNFNSAHDSNSNLSIDKYYDPAGKSLKMGQDSVWDYHVWNECWFVRRDLGNPYCGWQVIDATPQERSQGMYQCGPASVLAVKQGDVRLNYDTAFVFTEVNADINRWVVQSNGKRVKAYCDTSSIGRALSTKAVGSNQRVDVTNCYKFPEGSTEERSVYRKACMELSGSSHAEVAASELMAHPELFGKFKLTQSPVLGKDVNLILSLSNLSASPKTVTVNLSVSSVLYTRRAVKEILKETTTLHLGGKEEKHLPLRISYGHYGNSLTDDKKLLVTALCDVSEGVKLLVEKAITLESPNLSIAVPPKVFANTPATLEISYANPLPEPVKDCVLLVTLMGQAIKINVADLAAGERSKIYFEFTPRRTGAMQLHVDFSCSKFHHVKSFVLMDVAPAAGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z125</accession>
    <name>A0A6I9Z125_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3',5'-cyclic-AMP phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106555444</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.53" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013929771.1">
        <property type="nucleotide sequence ID" value="XM_014074296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z125"/>
    <dbReference type="GeneID" id="106555444"/>
    <dbReference type="KEGG" id="tsr:106555444"/>
    <dbReference type="OrthoDB" id="6111453at2759"/>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40141:SF2">
        <property type="entry name" value="3',5'-CYCLIC-AMP PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40141">
        <property type="entry name" value="3',5'-CYCLIC-AMP PHOSPHODIESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphodiesterase 4 upstream conserved regions (UCR)" evidence="7">
        <location>
            <begin position="157"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013929771.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="26543" checksum="9F9A4657EEA015FC" modified="2020-10-07" version="1">MRRSRTMSFLSMEKSREQLDLATCIGAFPGGGIKRRFSGTPLLPPLACRHATINDLSHGPEMEHSRVVFTIEESTPAAVKDSDLISFDVENGFSPGRSPLGSQANLGLGLVLQGTTPHSQRRESFLYRSDSDYDFSPKAMSRNSSVSSDMHGEDMIVTPFAQVLASLRTVRNNFAVLTHLREHVCNKRTSGPVNPLSTSKVNLSEDAYQKLAIETLEELDWCLDQLETLQTRHSVSEMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2C3</accession>
    <name>A0A6I9Z2C3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Stromal interaction molecule 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">STIM1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931208.1">
        <property type="nucleotide sequence ID" value="XM_014075733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2C3"/>
    <dbReference type="GeneID" id="106556748"/>
    <dbReference type="KEGG" id="tsr:106556748"/>
    <dbReference type="CTD" id="6786"/>
    <dbReference type="OrthoDB" id="9986177at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002115">
        <property type="term" value="P:store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09573">
        <property type="entry name" value="SAM_STIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000009">
        <property type="entry name" value="Stromal interaction molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057835">
        <property type="entry name" value="EF-hand_STIM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032393">
        <property type="entry name" value="SOAR_STIM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037608">
        <property type="entry name" value="STIM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037609">
        <property type="entry name" value="STIM1_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15136:SF9">
        <property type="entry name" value="STROMAL INTERACTION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15136">
        <property type="entry name" value="STROMAL INTERACTION MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25578">
        <property type="entry name" value="EF-hand_STIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16533">
        <property type="entry name" value="SOAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026649027" evidence="14">
        <location>
            <begin position="24"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="15">
        <location>
            <begin position="121"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="443"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="594"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013931208.1"/>
        </source>
    </evidence>
    <sequence length="608" mass="66555" checksum="37A06EBF3EC47FE9" modified="2020-10-07" version="1">MALWGRIALVLLCGLVLPPPGPAEVPPSPHSAPGPHLDQAGLEEFCHIDRPLCHNEDEQLSFEAVRSIHKQMDDDANGNVDVEESDEKWGASAEGRQEGGKHSVCVCLSLSLSAPSAVYNWTVEEVVQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGALLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFLLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERCAPSLPSEALWGPLCSPPPQVRMALRNAEKELESHSSWAAPGALQKWLQLTHEVEVQYYNVKKHSAEKQLLLAKEGPAAWAPWGWGRRVGAAALSCMLRPDRRKTHLLLPLGWLCHREREEREGGARGKAGWPPPSLTLPPGKAGMEGGVPWMGPVRCSLFLPNRPIFPPLPPAPTFPSSIVRQRLVDPQHSLGSQRDLARSDSDSSIPHLSELPRASASMPKLRPALLSKAAEEQSSQTEPPESESPLTMTKVPRANHHGGLEKAASLGELGSVGARSGDSSRSHSPSSTEPDTPSPSANGRTGLSLLSGKANSRIPHLAAKKGEEDSGSTGEETDSGAGKKKFPLKIFKKPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2K2</accession>
    <name>A0A6I9Z2K2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Kin of IRRE-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106556030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930459.1">
        <property type="nucleotide sequence ID" value="XM_014074984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2K2"/>
    <dbReference type="GeneID" id="106556030"/>
    <dbReference type="KEGG" id="tsr:106556030"/>
    <dbReference type="OrthoDB" id="6413693at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000170">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF49">
        <property type="entry name" value="KIN OF IRRE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="91"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="193"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930459.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="26417" checksum="F4528EC68026D43A" modified="2020-10-07" version="1">GHRLSSYHHLVLCAIPEYNGIVLWIKDGLALGVGRDLSSYPRYLVVGDHLSGEHHLKILRADLQDDAVYECQAIQAAIRSRPARLTVLVPPDDPIIIGGPIISLRAGDPLNLTCHADNAKPAATIIWMRRGEVINGATYSKTVVRDGKRESIMSMLFVSPSDIENGQIIVCRATNKAVPSGKETSVTIDIQHPPLVNLSVEPQPVLEDNTVKFHCSAKANPAVTQYRWAKKGHIIKEASSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2W5</accession>
    <name>A0A6I9Z2W5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein LBH-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930564.1">
        <property type="nucleotide sequence ID" value="XM_014075089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2W5"/>
    <dbReference type="GeneID" id="106556119"/>
    <dbReference type="KEGG" id="tsr:106556119"/>
    <dbReference type="OrthoDB" id="9421103at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038990">
        <property type="entry name" value="LBH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042945">
        <property type="entry name" value="LBH_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14987:SF3">
        <property type="entry name" value="LBH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14987">
        <property type="entry name" value="PROTEIN LBH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15317">
        <property type="entry name" value="Lbh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LBH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930564.1"/>
        </source>
    </evidence>
    <sequence length="116" mass="12999" checksum="B5CB61CE5E4F1C95" modified="2020-10-07" version="1">MTEVMNTREPVMEEFTLSQPEEEGGTSTQAFPDAHEKYPKLSKRLPSIVVEPSEMGDVESGELRWPPEDLKSMEDKKALTAQRSYAQQHQMDLESASPHQDIGDSTKSSGSRTEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z300</accession>
    <name>A0A6I9Z300_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylinositol 4-kinase alpha-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106556153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930604.1">
        <property type="nucleotide sequence ID" value="XM_014075129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z300"/>
    <dbReference type="GeneID" id="106556153"/>
    <dbReference type="KEGG" id="tsr:106556153"/>
    <dbReference type="OrthoDB" id="9037772at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013930604.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="14041" checksum="8D6E6AF6B59AA7A8" modified="2020-10-07" version="1">MCPVDIHGIFQLDERRRDAVIALGIFLIESDLQHKDSLIPYLLRLLRGLPKVQWIEESNSRKGRGTLPVAENFSFCLVTLLSDVAYRDASLREQVLEPILQVMQVLLGMCQSPQIQDKGMFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6Z6</accession>
    <name>A0A6I9Z6Z6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein ADP-ribosylarginine hydrolase-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106557080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931671.1">
        <property type="nucleotide sequence ID" value="XM_014076196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6Z6"/>
    <dbReference type="GeneID" id="106557080"/>
    <dbReference type="KEGG" id="tsr:106557080"/>
    <dbReference type="OrthoDB" id="10250509at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003875">
        <property type="term" value="F:ADP-ribosylarginine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051725">
        <property type="term" value="P:protein de-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4080.10:FF:000002">
        <property type="entry name" value="ADP-ribosylarginine hydrolase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012108">
        <property type="entry name" value="ADP-ribosylarg_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF39">
        <property type="entry name" value="ADP-RIBOSYLARGININE HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016939">
        <property type="entry name" value="ADP_ribslarg_hdr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931671.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="39436" checksum="6D8F10DFF9FC0813" modified="2020-10-07" version="1">MDGSYCTPSQEAYRAAMVLSGVGDALGYRNQRWEYCTSGPQIHQELQEMGGLGKIHVILPDWPVSDDTVLHLATAEALATGKTGESLFQELARCYVEAMKDMEGRKPGPTSILGTSQLRPGEPGGYHIPFNSYATGCGAAMRSMCIGLRYPQPSDLNTLIKVSIESGRMTHHHPTGYLGSLASALFTAYAVQGLPLELWGCGLLKTLPLALEYIQAAGIETEANVAAWPYFQEKWEWYLSERGLIDGRGPVRFPPAYGPAERDVIYKTFSLDGWAGRSGHDAPMIAYDALLGAGSSWEQLCGRSMFHGGDSDSTGVIAACCWGLTYGLQDIPSGNHMELEYRDRMVSAADSLYRLAWEQGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZH8</accession>
    <name>A0A6I9WZH8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">A-kinase anchor protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AKAP11</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AKAP110 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908379.1">
        <property type="nucleotide sequence ID" value="XM_014052904.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106538423"/>
    <dbReference type="KEGG" id="tsr:106538423"/>
    <dbReference type="CTD" id="11215"/>
    <dbReference type="OrthoDB" id="9943913at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018292">
        <property type="entry name" value="AKAP_110_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008382">
        <property type="entry name" value="SPHK1-interactor_AKAP_110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10226">
        <property type="entry name" value="A KINASE ANCHOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10226:SF3">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05716">
        <property type="entry name" value="AKAP_110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026708802" evidence="4">
        <location>
            <begin position="24"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="domain" description="A-kinase anchor 110kDa C-terminal" evidence="5">
        <location>
            <begin position="1712"/>
            <end position="1781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="967"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1644"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1646"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1689"/>
            <end position="1698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013908379.1"/>
        </source>
    </evidence>
    <sequence length="1806" mass="200224" checksum="603E1160654A589B" modified="2020-10-07" version="1">MEENCVFFLTNWVTFLCFASEAALPPKNCVSRVLCVMRLSPSFARLRGDAVLTLLGKYTAAIRCSEEMRSLQKQTPDSTHTENDDTNQSVSSIEDDFVTALEHLEEDEPGKTTHIETEKSPESSPKSTFAHPLKSNDQKSFVSSLYRRPSVKSSISLVNILELEELSSSARNPVSTSISEPWMQRNFFRPHSSSDRGVNFLHKTLFSSSPADSSESDCSSPSPVIFLDEEGYQKSLKAKLQLPKIPVGKDGVEDSDSEVSEFFDSFDQFDEREQTLENSSTGSRDPATVSHTSQKSIVSCQKSCSTSASMNPQKFKCDRPVLPANVRKPTPRKPESPYNNLLDVPDSPRLIKNPADDHAALFSPIRSSAFSPPGSCFSAECLCQVNRDAFSQNHNIVYHTYSDFANNISFEILGSVCNARPPSLCRCTQEMIGQGRVVPEEQKDRTAKIQRDISRKGLHKKDRSKHKSGVIKDHIQRFASELVETSFGSAFKDLQKSVSSCTNALCQLAAKLTSSVFQMAFYEIGRQQALLLKKKAINGLAGFLVSEAVTGALKELYSVKQQMFTNTVTKFAANLAEELIFEGIMEVCQFSHPPTPTASHQDSFRYNDRVVWSYAQDLSESVIQDAFIELSQVDVSFTSQAAISVSTDNVKCVSKEKVLELTHTSNGSYELGEKVPVALNPVWEFRKEHTINRALFYTTGIASSIPVPLAGSILSQHQILWKEDNVKNPLPSETKLSYVRGSPEMCCATKNNRSEVSSLRNIYLTTDFTAGNEHDSPDHPESGKFAEISSTNNFSGTMVDMIVSEVYEAVTSSRVSKSAETYTGVLQMNDAPCLQCIGKDSCKNMFANYLAKRIVKQSVDETKSACSATSEKLAYCVGLGTHRRSGKTESQGAAKQGEKTKNIPVIVGQQQMPLNNISKFHISTAASNRCLLLGSKEGVQERKNCVACKPSASIISPTMTFVKSNEQVPDAEDSSAIPISNLPEKQSTSKAPGGSAFGHEMPFPEISNSSVVPTCEDALQLEDKLSVRDTNLLVVPETPPSTPLMPSQASSEWNLRKLTKKLKGELAKEFAPATPPSTPYVPETDGIYTPECEKLEKEEFMLKLMRSLSEEVENKEVEEHPETSLEQIQPLGPTVDYADHLATRIISMATEQAASRLEDKATQMEKKRYFQVSTEDQRGGHSTFVNVPEKHPPTLWIYAGEMAGRVIREAKKRLNSRHCKLLSLRHVNYEGSCFHLKRNYHECWSKDQRPKPCSREPDPCALPVPLNSETSGLTSKYPSCESVTDEYTDHIIRVLKQEGGHGDLIMDNFASRLAYKTVRSGMQQAARKLRAKPSRKIFPEKSSKLNSKLELFEAANQDFDQKSQKNGVMLHLTKPWRSRCPLHGPERSKLLDFSESLACTITSDARRMFMSAASLPKSFTDSCLYQPPRDDKVTDFKTTWSQRLSSSRCKQKLYHSMESLRDSTYQGNILRAVDQYAKKIVDDTLEVSLGSATRQAPEGWTNGERLTYEEKTIPFSIAACKHCSLKEHRTCIGGSSLPLAGRDLPSNVRQSSDGSVSITLPKSYVLHLDIPKIHVDMEEKVLFAENLAPVTLDRARRQLSNTSLTADSGISQDGISFAESLSTEIMTSCMTNIAPAISISAMETEGPNSAESVVSQQTGEDSTGSWSNLSFDEEHPDESSSFLHLSDSDGAEDKEEELNASMEGLRKPSKALVIVNVDVGPCAVDPQLRMTLQWLSASEADVPELRFHEDTPGEFLLLSKTLQEKGWRVGDLFQAVLKYCEAMEKSSDGDRTLGSPTLFRWLQENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X044</accession>
    <name>A0A6I9X044_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CD82 antigen-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106537956</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907741.1">
        <property type="nucleotide sequence ID" value="XM_014052266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X044"/>
    <dbReference type="GeneID" id="106537956"/>
    <dbReference type="KEGG" id="tsr:106537956"/>
    <dbReference type="OrthoDB" id="10016273at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF44">
        <property type="entry name" value="CD82 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013907741.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="21858" checksum="817932EF295166AF" modified="2020-10-07" version="1">MWHCATGFTAGTSLAIKPNGTVTHRNVLPLLNCTIINRNFPECPETFILGAVILGFGIWILVDKTSFISVLQTSSSTTKVGAYILTGVGALTMLMGFLGCVGAVNEIRCLLGLYFTCLLLILIAQIAAGLLIYFQRNALKAEMSGIVSELVQNYNPYDETNRTLENAWDYVQLQVSCYVRVNETVSDIVNNTYGKLFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0L5</accession>
    <name>A0A6I9X0L5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106537655</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907322.1">
        <property type="nucleotide sequence ID" value="XM_014051847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0L5"/>
    <dbReference type="GeneID" id="106537655"/>
    <dbReference type="KEGG" id="tsr:106537655"/>
    <dbReference type="OrthoDB" id="5967130at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15915">
        <property type="entry name" value="7tmA_OR12D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013907322.1"/>
        </source>
    </evidence>
    <sequence length="310" mass="35378" checksum="49A79191054FD30A" modified="2020-10-07" version="1">MRNSTEVSEFILLGLTRDHRLQQVLFPLFLLLYIACLLGNGAILIVILTEPRLHIPMYFFLGNLSCLDICYSTVTVPKMLRGFLRKHQSISFNGCLIQLHFFHFLGCSEAVLLGIMACDRYVAICKPLHYTLIMNKWTCVILAGVTWTIGFFYALMHTIVTSQLVFCGPKLVPHIFCDIKPLLVLACSSTRLSYTLLNFVTVSIAFSSFFFTLLSYFYIISFLVLKVQSWQNRWKAFSTCASHLMVVAMFYIPVLSNYMLASASDKDPIVSLLYSIVTPVLNPLIYSLRNYEVKNAIRKILIQNAIFVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3C5</accession>
    <name>A0A6I9X3C5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Uncharacterized protein LOC106538391</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6" type="primary">LOC106538391</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908338.1">
        <property type="nucleotide sequence ID" value="XM_014052863.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908339.1">
        <property type="nucleotide sequence ID" value="XM_014052864.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908340.1">
        <property type="nucleotide sequence ID" value="XM_014052865.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908342.1">
        <property type="nucleotide sequence ID" value="XM_014052867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3C5"/>
    <dbReference type="GeneID" id="106538391"/>
    <dbReference type="KEGG" id="tsr:106538391"/>
    <dbReference type="OrthoDB" id="9014577at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013908338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013908340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013908342.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="34164" checksum="21959F246D52C3AD" modified="2020-10-07" version="1">MAALDKIKAKKTLGTVDYVESSEFAQGILPTKKDVIQNMLYLLHPKRAGQAQRSKEDAAQLLAELLQEHWLFCNLYTIATQSIKKHILKVYEEFSKLYQSRKRRKNELFIQKADDFNRSSEQLFDIFCTDTVTREKLEQCSGVKMTDTEWKFLEDQRNERKMYFEDFVDKKQMETIERRRKVESLENFRKIAEEEKEARKRKEMTSNRDEQSVEDVNKNKDDSCLEYNKGIGGFSDKAKRKRLSSCWVTGTATSTTYNESVPSENQHLRMNIRKVRPGFYETFDKYKNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6D0</accession>
    <name>A0A6I9X6D0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Transmembrane protein 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12 13 14 15 16 17 18 19" type="primary">LOC106539193</name>
    </gene>
    <organism evidence="8 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909388.1">
        <property type="nucleotide sequence ID" value="XM_014053913.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909389.1">
        <property type="nucleotide sequence ID" value="XM_014053914.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909390.1">
        <property type="nucleotide sequence ID" value="XM_014053915.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909391.1">
        <property type="nucleotide sequence ID" value="XM_014053916.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909392.1">
        <property type="nucleotide sequence ID" value="XM_014053917.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909393.1">
        <property type="nucleotide sequence ID" value="XM_014053918.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909395.1">
        <property type="nucleotide sequence ID" value="XM_014053920.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909396.1">
        <property type="nucleotide sequence ID" value="XM_014053921.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909397.1">
        <property type="nucleotide sequence ID" value="XM_014053922.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909398.1">
        <property type="nucleotide sequence ID" value="XM_014053923.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909399.1">
        <property type="nucleotide sequence ID" value="XM_014053924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6D0"/>
    <dbReference type="GeneID" id="106539193"/>
    <dbReference type="KEGG" id="tsr:106539193"/>
    <dbReference type="OrthoDB" id="9026846at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008853">
        <property type="entry name" value="TMEM9/TMEM9B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13064:SF1">
        <property type="entry name" value="PROTON-TRANSPORTING V-TYPE ATPASE COMPLEX ASSEMBLY REGULATOR TMEM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13064">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05434">
        <property type="entry name" value="Tmemb_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636573" evidence="7">
        <location>
            <begin position="21"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013909388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013909389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013909390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013909392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013909396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013909398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013909399.1"/>
        </source>
    </evidence>
    <sequence length="136" mass="15655" checksum="4401628038FE811E" modified="2020-10-07" version="1">MKSMSLAAIFLCILIPSFETKQFMPNGCYCFCPPYQNLSAMTFWKKEFQDDCNCLYTMEPMPVPRHEAEAYCLLCDCKFVERNNPATKIILIVLLSVFGAVLLLKVFSVVVKYFIHKPDAYTKPLHNEEVSEDPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X854</accession>
    <name>A0A6I9X854_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc finger protein ZXDC-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106537787</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013907508.1">
        <property type="nucleotide sequence ID" value="XM_014052033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X854"/>
    <dbReference type="GeneID" id="106537787"/>
    <dbReference type="KEGG" id="tsr:106537787"/>
    <dbReference type="OrthoDB" id="6277246at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000257">
        <property type="entry name" value="ZXD family zinc finger C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN ZXDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="359"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013907508.1"/>
        </source>
    </evidence>
    <sequence length="424" mass="43521" checksum="D24641BFFFB69CFC" modified="2020-10-07" version="1">METQGLPAAAEAAQPEPATQQQHGAGAEDGAAPSGPVRRPPQPRDSAAAAGAWGLDVTFPVLLLLEGKLPETPQPHQQLPPPPPLPPPPPPGPANSGGASSPPAGGPGQPPESDALLLVLNLVRGGASGSPSRGSSQDSPPAQRPREGKPGGEAGGESQASAPLSEGGGPQEESGGGGSQGNSSFSGTLTINNQSLLVRFENGTLSFGGPAPAAPPPAPAQPSDDAQPPAALSRAPASYPPRPDSHRADTFSRKQLLRLHQLSARGGSPTGAPQEGGEAVAAAGGLGGSAAAGRVARPFSCPIPGCSWAFATAYKLRRHLHSHDKLRPFACSAPGCTKRFTTIYNLRAHGRAHEEESRLKCEACGQRFPSAARLGAHQRRSHLEPDRPYRCEYPGCERTFITEKDLSFVILKPVAGPSPVCLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8B9</accession>
    <name>A0A6I9X8B9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Waprin-Enh1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106539706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910032.1">
        <property type="nucleotide sequence ID" value="XM_014054557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8B9"/>
    <dbReference type="GeneID" id="106539706"/>
    <dbReference type="KEGG" id="tsr:106539706"/>
    <dbReference type="OrthoDB" id="9029120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026868347" evidence="1">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="2">
        <location>
            <begin position="72"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013910032.1"/>
        </source>
    </evidence>
    <sequence length="116" mass="13160" checksum="5665E94AAEC9435D" modified="2020-10-07" version="1">MKTLTGLLLVGLLALWIELPSTSSKVLFACGTSLGNPFPCSLPGINRCRSDYECPHTLRCCNFRCSRSCRTPPVIPWSCPRNPFKCTIPGIHRCRYDYDCPGRQRCCYYNCSRICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XA06</accession>
    <name>A0A6I9XA06_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein SFI1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SFI1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911156.1">
        <property type="nucleotide sequence ID" value="XM_014055681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XA06"/>
    <dbReference type="GeneID" id="106540527"/>
    <dbReference type="KEGG" id="tsr:106540527"/>
    <dbReference type="CTD" id="9814"/>
    <dbReference type="OrthoDB" id="195843at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052270">
        <property type="entry name" value="CACF_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028:SF4">
        <property type="entry name" value="PROTEIN SFI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911156.1"/>
        </source>
    </evidence>
    <sequence length="655" mass="80040" checksum="22DB8D932D87A9BB" modified="2020-10-07" version="1">MERQGRGSSKFQKAQKPHKECVGRQPIPSHPNRNSGQSGNCRILYRVMYTWNRGGRLKELRIRHLARKFFYLWKRKAFGRVLPSKARAYFVQKTLQKTFGEWKEEWWVLCREWKLTVRADCRHRYFLYHKVFQAWRSYLLQKRAKKLAYYTAEAHAAKQKTLWSWQHWLSYIQLCRMKQRMHWEALHFWQQSTLRLSWGLWQRRWRQRQAYRGMESQALQQWAHSVQLCAWLQWKALDTQLQQERATAGWAARRLQHWTMWKGLKPWLQYVDQRREKQRRSRLASQHHHAQLLRWHFSAWQLAWKHKKQSEHLAQQVAGTILRRAFVQWKLYVTLCAEEAEHYGLAQSHHNHRLLSFGFHALRRNIQEASLKQVRRNLACQQHRVMLTSRFWDCWTSRLEQKEDEQHRLLTLAAASHHRRRLLQKSLWTWREGACGERWWKFQWAKAEQHRGAVVLSAAFQAWELFKDHQLWWRQMNGVAAGYHRETWTRRVFVQWLLRQCEHQEGRAAEKMATLHAGQRLLSQFWGQWHRATLACLEEREGVSLAKGHHRHRLLWATLHLWRENVWEMKRGRAKEAAALHFRSEKLLWHSWRKWRQYQMQKSEKWKKMARAEGHYQQVLLGRVLRAWKAYQTSVQGVLHQVAQKERDYRRQLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAC5</accession>
    <name>A0A6I9XAC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106540611</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9 10">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013911266.1">
        <property type="nucleotide sequence ID" value="XM_014055791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAC5"/>
    <dbReference type="GeneID" id="106540611"/>
    <dbReference type="KEGG" id="tsr:106540611"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000048">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF20">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="5">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="218"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="448"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911266.1"/>
        </source>
    </evidence>
    <sequence length="579" mass="64667" checksum="D90B709FF4083462" modified="2020-10-07" version="1">MKQQLHQQQHSCTLSECFQLYTKEEQLAPDDAWRCPHCQVLQQGMVKLSLWTLPDILIIHLKRFRQVGEQRHKLSTLVHFPLRGLDMAPHVAKRSRGSKWGPWRQPLPEEGQCNFLYDLYAVCNHHGSLHGGHYTAYCCNSLDGRWYSYDDSTVEPVMEEDISTRGAYILFYQRRNVVPSWPEGSSVRGSTSSSISEHWLTRLSRSSKRESLVSWSSTTCPSLPKSSHSPTLLSHSPTSQEKGGFESRPLVRGVQGRSVSLKASSNKAKLGIPKTLPLRWSFTSKDWPSEPSRELVDYLESGRRPWFTKESIVPLITKEGEDNELTVTKANPALTSMGPTETSCKTATAMENLNTLKRVVKPKEETGEQPRTPKKLVLSLSTVPPVKDEEPKTKANINGTLRIGSSVTSSSRGDAEQKTGPWLREKEPENEKQSEGKLAVFRVGFLRKDPKRHSESPRHAVMTSSTASSQASFSNGTLNIIPGSRTNNALNPRSTEGLSNGRISRSEVDIKRAQSSCNSKGRNDWLLNRSTTLCKANNGTLQASMTPLPLEGISCGTFQRARYHTASLGRRIIVPESSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XB32</accession>
    <name>A0A6I9XB32_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calmodulin-lysine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CAMKMT</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the trimethylation of 'Lys-116' in calmodulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[calmodulin]-L-lysine + S-adenosyl-L-methionine = [calmodulin]-N(6)-methyl-L-lysine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21556"/>
            <dbReference type="Rhea" id="RHEA-COMP:11360"/>
            <dbReference type="Rhea" id="RHEA-COMP:11361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.60"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.60" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013908408.1">
        <property type="nucleotide sequence ID" value="XM_014052933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XB32"/>
    <dbReference type="GeneID" id="106538446"/>
    <dbReference type="KEGG" id="tsr:106538446"/>
    <dbReference type="CTD" id="79823"/>
    <dbReference type="OrthoDB" id="413520at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018025">
        <property type="term" value="F:calmodulin-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000140">
        <property type="entry name" value="Calmodulin-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025800">
        <property type="entry name" value="CaM-Lys-N-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13539">
        <property type="entry name" value="CALMODULIN-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13539:SF3">
        <property type="entry name" value="CALMODULIN-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51610">
        <property type="entry name" value="SAM_CLNMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013908408.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="36155" checksum="46C1FF81B02315C8" modified="2020-10-07" version="1">MAAESSPSASPAPADRAQAENQEGDAGERSKQATAKARWTLLRQVLKQKPLENANLQQISVRRFASFGLFSITEDSNMTEDDSRLWVQYKSNYYPDYIISLRCNSRLLNVEDVLTSFDNTGNVCIWPSEEILAYYCLKHHEIFRGLAVCELGGGMTCLAGLMVAISADVKEVLLTDGNEKAIQNVCDIITRNKKAGVFRAKAISSSILRWDNETDVSQLEGHFDIVMCADCLFLDQYRASLVDAIKRLLHHSGKALIFAPLRGDTLNQFCSLAEKAGFSIQRHENYDERISNFHSKLKKEEKDIYDPNLHYPFFLVLTKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XE77</accession>
    <name>A0A6I9XE77_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="4 10">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UGP2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19890"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="4 10"/>
    <dbReference type="RefSeq" id="XP_013912501.1">
        <property type="nucleotide sequence ID" value="XM_014057026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XE77"/>
    <dbReference type="GeneID" id="106541541"/>
    <dbReference type="KEGG" id="tsr:106541541"/>
    <dbReference type="CTD" id="7360"/>
    <dbReference type="OrthoDB" id="932129at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912501.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="56759" checksum="2EB01B45F4450B7B" modified="2020-10-07" version="1">MSSFAKDFSKAMSQAGTSPFQEAIRQELEYSMKMELDKILGTAPQNEVQHTKKDLEGFQKLFRRFLQEKGPSVDWEKIQRPPEDSIQPYEKIKARGIPDSIASALNKLVVVKLNGGLGTSMGCKGPKSLIGVRNENTFLDLTVQQIEHLNKTYNTDVPLVLMNSFNTDEDTKKILQKYSHSGVKIHTFNQSRYPRINKESLLPVAKDVSYSGENTEAWYPPGHGDIYASFYNSGLLDIFIEDGKEYIFVSNIDNLGATVDLYILNHLMNSPNGKRCEFVMEVTNKTRADVKGGTLTQYENKLRLVEIAQVPKAHVDEFKSVSKFKIFNTNNLWISLSAIKRLQEQNAIDMEIIVNPKTLDGGLNVIQLETAVGAAIKSFENSLGINVPRSRFLPVKTTSDLLLVMSNLYSLNAGSLTMSEKREFPSVPLVKLGSSFTKVQDYLTRFESIPDMLELDHLTVSGDVTFGKHVALKGTVIIIANHGDRIDIPPGAVLENKIVSGNLRILDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XER2</accession>
    <name>A0A6I9XER2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106542829</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914144.1">
        <property type="nucleotide sequence ID" value="XM_014058669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XER2"/>
    <dbReference type="GeneID" id="106542829"/>
    <dbReference type="KEGG" id="tsr:106542829"/>
    <dbReference type="OrthoDB" id="6147321at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000005">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013914144.1"/>
        </source>
    </evidence>
    <sequence length="304" mass="34539" checksum="B24CEDD7A7374A04" modified="2020-10-07" version="1">MMNQTSVTEFLLLGLSRNLRIRLFLLGLFSIVYVLTLVYNTIILMLIWLDMRLHTPMYFFLGQLACVDICYTSSTVPQMLANLQSPRQTISLAACAIQMHIFLALAITECILLAVMAYDRYVAICHPLHYVFLMKKTVCMKLALICWTTGFLLPVVHTVLTWQLPFCGSNLIDHFFCEMPALLKLACADTQMVQTVTSMGCIFTLLIPISFILMTYIRILVAILKIQSVQGQRKAFFTCGSHMTVVVLFYGSAIFMYMRPESSHSSGQDKIVSLFYSIVAPIFNPMIYCLNSKTMKDAFLKMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XER5</accession>
    <name>A0A6I9XER5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein lin-37 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LIN37</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912681.1">
        <property type="nucleotide sequence ID" value="XM_014057206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XER5"/>
    <dbReference type="GeneID" id="106541690"/>
    <dbReference type="KEGG" id="tsr:106541690"/>
    <dbReference type="CTD" id="55957"/>
    <dbReference type="OrthoDB" id="6287771at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031523">
        <property type="term" value="C:Myb complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028226">
        <property type="entry name" value="LIN37"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31336">
        <property type="entry name" value="LIN37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31336:SF3">
        <property type="entry name" value="PROTEIN LIN-37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15306">
        <property type="entry name" value="LIN37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912681.1"/>
        </source>
    </evidence>
    <sequence length="182" mass="20824" checksum="D7C7504C49F070F8" modified="2020-10-07" version="1">MLHTKVKTEKPDLETANARNRLDAVLQCLLEKTDVDRDQMEEDPGKTSSDALSKDPSPIATGKRPSTRFSHHRRKKRKEMDDGLTEGGPQKQNTYIIKLFDRSVDLAQFSENTPLYPICRAWMRNSPMVREQERSPSPNLPTLIEDEEGVEVLNGKIQDVYKLPSPLHCRSYISLRIFMLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XF04</accession>
    <name>A0A6I9XF04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Polycomb group protein ASXL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ASXL2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912761.1">
        <property type="nucleotide sequence ID" value="XM_014057286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XF04"/>
    <dbReference type="GeneID" id="106541757"/>
    <dbReference type="KEGG" id="tsr:106541757"/>
    <dbReference type="CTD" id="55252"/>
    <dbReference type="OrthoDB" id="9348951at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035517">
        <property type="term" value="C:PR-DUB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042975">
        <property type="term" value="F:peroxisome proliferator activated receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024811">
        <property type="entry name" value="ASX/ASX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028020">
        <property type="entry name" value="ASX_DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007759">
        <property type="entry name" value="Asxl_HARE-HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13578">
        <property type="entry name" value="ADDITIONAL SEX COMBS LIKE PROTEIN ASXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13578:SF11">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN ASXL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13919">
        <property type="entry name" value="ASXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05066">
        <property type="entry name" value="HARE-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51913">
        <property type="entry name" value="HTH_HARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HTH HARE-type" evidence="9">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="10">
        <location>
            <begin position="275"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="450"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="450"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51913"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013912761.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="52713" checksum="FE8E59101982543F" modified="2020-10-07" version="1">MREKGRKKKGRTWAEAARTVLEKNPNRPMSHKEILQVIQKEGLKEIRSGTSPLACLNAMLHTNSRGDEGIFYKVPGRMGVYTLKKDVPDGLKDLSEGSEESSDIQSDSPTSESSSSSSSDQSSNKEGRKSRWQRKVSARLSQVSSPQPGCPSPSIPPGKIISSSQKHSKKALKQALKQKQRKQHHCRTSVPVSTNHHLLQQHVKIANNPAKAAWEGKQSDGHSSSPQNSTSSSSPSVKTEPSLPILGKKPFQRSERLHARQLKRTKCAEIDVETPESILVNTNLRALINKHTFMGLPVECQQKLLLLLPEVDRQIGMDGLMKLSSSALNNEFFNSAAQGWKERLSEGEFTPEMQLRLRQELEKEKKVELWKERFFESYYGQSSGLSLEESKQLTSCIPTVQNQAQTGNLAPQLQKCIVVHKAVPDKEQTNPLVGPKVQVLPAVTAATKGEIEQPKVFKPEELAHSSGNSASFHSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XF53</accession>
    <name>A0A6I9XF53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Opsin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106541544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
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            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912507.1">
        <property type="nucleotide sequence ID" value="XM_014057032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XF53"/>
    <dbReference type="GeneID" id="106541544"/>
    <dbReference type="KEGG" id="tsr:106541544"/>
    <dbReference type="OrthoDB" id="2105199at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912507.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16899" checksum="A493855128CB24F0" modified="2020-10-07" version="1">MCFIMITTFLICWMPYAVVSLLIAYGYAHLITPTVAIIPSFFAKSNTAYNPVIFIFMNRKFRQCLTQLLCVHLLRFQRILKEKPAIRNEKPVRPIVVSQKVRDRPKKKVTFSSSSVIFIITNDETQQIDDSSKDIGTKGNMIQVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFF0</accession>
    <name>A0A6I9XFF0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Ficolin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106539505</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Initiates complement activation and/or interferes in platelet aggregation and/or blood coagulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ficolin lectin family. Veficolin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909778.1">
        <property type="nucleotide sequence ID" value="XM_014054303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFF0"/>
    <dbReference type="GeneID" id="106539505"/>
    <dbReference type="KEGG" id="tsr:106539505"/>
    <dbReference type="OrthoDB" id="7735550at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001867">
        <property type="term" value="P:complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF433">
        <property type="entry name" value="FICOLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0176">Collagen</keyword>
    <keyword evidence="15" id="KW-1216">Complement system impairing toxin</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="17" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026920206" evidence="19">
        <location>
            <begin position="23"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="20">
        <location>
            <begin position="114"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="46"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013909778.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="36881" checksum="7C5B8795AC1333E0" modified="2020-10-07" version="1">MRRAGEQVGLLLFCLMIATCRGAQESSCPELKELGLDGNETIAIVRGSQGLPGPQGPQGPPGNPGAKGERGLQGIPGEIGAPGPKGEKGEPGQPGEKGKKGDPGEIDEKKLEALQCKQGAPNCKELLKRGMILSDWYTIYPQDCQPLQVLCDIVTDVGGWIVFQRRSDDSVDFFRDWAAYKKGFGNQLTEFWLGNDNLHRLTSLGKNELRVDFTNFENQHSFAKYASFQVTAESDRYRLTLGVFTGGSAGDSLSYHNNMPFSTKEKRQDPRKFNCAEKCQGAWWYHECHHSNLNGRYWLGEHKTRGNGINWATGKGPYYSYKRSEMKIRPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGP0</accession>
    <name>A0A6I9XGP0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Contactin-associated protein-like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CNTNAP4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910203.1">
        <property type="nucleotide sequence ID" value="XM_014054728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGP0"/>
    <dbReference type="GeneID" id="106539829"/>
    <dbReference type="KEGG" id="tsr:106539829"/>
    <dbReference type="CTD" id="85445"/>
    <dbReference type="OrthoDB" id="26719at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000005">
        <property type="entry name" value="Contactin associated protein-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000026">
        <property type="entry name" value="contactin-associated protein-like 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF40">
        <property type="entry name" value="CONTACTIN-ASSOCIATED PROTEIN-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1124"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="65"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="252"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="431"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="16">
        <location>
            <begin position="467"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="675"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="841"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="891"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1089"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1089"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1102"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="813"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013910203.1"/>
        </source>
    </evidence>
    <sequence length="1191" mass="132631" checksum="8AB23FDB25253D2B" modified="2020-10-07" version="1">RPEQNAWAPSSSLAHAPKSLKLNNFEPRGGSWGCPEFGGPEEPSPWRGSPLLGDPGLHNFGGSDVADFDGRSFLLYRFKPKSTTALKDVISLKFKTSQRDGILLHREGQNGDHITLVLANGKLSLFINIGDAKMYSADAQINITLGSLLDDQHWHSVLIEHFNNQVNFTVDKHTHHFHAKGEFNYLDLDYELSFGGIAMPGKSGILPWKNFHGCFENIYYNGVNIIDLAKRYKPQINLMGNISFSCSEPPIIPVTFLNPNSYLALPGTAGLDEISVSFHFRTWNNEGLLLSNKLYQTSGGLLMYLSDGKVKLSFYKPGKAQSDITAGAGLHNGQWHSIYLLTKKNRVTLMVDNEAMSTSHMLVPMHIFSGDTYYFGGCPINVNNSECKNPYSSFLGCMKQISIDGKPIDLISVQQNTMGHFSDLQIDLCGIIDRCLPNYCEHGGECSQSWNSFYCNCANTGYTGATCHYSIYEQSCEAYKHRGNISAFYYIDSDGSGPLGPFLVYCNMTDTTWTIIQHNNTELTRVKTANRENPHSVFFKYAASLEQLQATINHAEQCEQEVAYHCKKSRLSNKQNGIPFSWWIGKINETQTYWGGSTPDVQKCACGIEGSCIDSEHDCNCDADRNEWTNDTGLLSYKDHLPVTELVITDTGRPNSEAAYKLGPLICQGDREFWNAASFNTEASYLHFPTFHGELSADMSFFFKTTASSGVLLENLGIRDFIRIELHSPLEVTFSFDVGNGPNEITVQSPIPLNDNQWHYVKAERNIKEASLQVDQLPQRTYSAPSDGHIRLQLNSQLFVGGTASRQKGFLGCIRFLQLNGRALDLEERAKVTPGVKPGCPGHCSSYGNLCYNGGKCKEKYNGFSCDCTFSAYTGPFCKKEISAYFGLGSSVTYNFQESYNVAKNSSSHAASFHPDMTLTREAITLNFRTTRIPSLLLYVNSFYKEYLSVILAKNGSLQIRYKLDSHRDPDIFSINSKSMADGQLHRVKIKREEEKLFVEVNQHERMKFFLSSDIQFNTIKSLFLGKILAETSDVDPDTLKANSQGFIGCLSSVQFNHVAPLKAAVHYPSSAPVIVKGHFMESNCGTLSGADTTSSETTHSFADHSGPRDEGEPLANVIRSDSAIIGGVIAVVIFIPLCILAIAVRIYKKKNVYKKKEVKGSENEDSAETVLKNEINVQNTINENQKEYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGV4</accession>
    <name>A0A6I9XGV4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Nuclear factor of activated T-cells 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">T-cell transcription factor NFAT5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">NFAT5</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer when bound to DNA, completely encircles its DNA target. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm. Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913346.1">
        <property type="nucleotide sequence ID" value="XM_014057871.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542209"/>
    <dbReference type="KEGG" id="tsr:106542209"/>
    <dbReference type="CTD" id="10725"/>
    <dbReference type="OrthoDB" id="5346094at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01178">
        <property type="entry name" value="IPT_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07882">
        <property type="entry name" value="RHD-n_TonEBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000174">
        <property type="entry name" value="Nuclear factor of activated T-cells 5, tonicity-responsive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000002">
        <property type="entry name" value="Nuclear factor of activated T-cells 5, tonicity-responsive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015646">
        <property type="entry name" value="NFAT5_RHD_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF10">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RHD" evidence="22">
        <location>
            <begin position="245"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="104"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="620"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="712"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="888"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="944"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1137"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1211"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1336"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="160"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="620"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="642"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="722"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="898"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="944"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1211"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1233"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1256"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1309"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1341"/>
            <end position="1395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013913346.1"/>
        </source>
    </evidence>
    <sequence length="1486" mass="158141" checksum="964C4191A1F1B756" modified="2020-10-07" version="1">MLVVFSINTLNLHSSFAIQRQASCGGLCAINSEETLNPPNLTQGIDASSAPSSSVGGACSSFTTSSGPTIYSTSVTDSKAMQVEGCSSAMGVSNRGVSDKQLTNNTVQQQQQPQSTPKRHTVLYISPPPEDLLDNSQMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGIKSRDGEESTMDIFDADSAKAPHYVLSQLGTESKSNLKAGNGTSEVPKAAGGKKSPLLCGQYLSKSEGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNDPVTLQIFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDVEGTTVIEVGLEPSNSMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIPRKDGSALTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGGEEVFLIGKNFLKGTKVIFQENISDESSWKAEAEIDMELFHQNHLIVKVPPYHDQQITTPVSVGIFVTTNAGRSHDIQQFAYTPDTSAETLNVNVKQEISSSTQPCSFDDVAKVNSSGCKLDQVNLLPSALVTPLRPNSIVKNEEAAPMELLAEKMSLPAFNTTSAIGQSQTTLETTISSLLGSGPFSSSASHLAPESEKQPPQPQYPPDTRPALQAPDLVQSGSFAATSAPSQLQNSDSLLQQASQFPLREASSREALQSDSTAMALSQLTEAPQQPPPLQESSQALQQQQQQQQISSSMFSSAGGVSQLQSTIQQLQAGSFQSSPGDGGGGSSRNIDLVQQVLEAQQQLSSVLFSGSEGEDVQEQLNAEIFQQVSQIQNGVNQGMFPSADTVHSRAEDLLPSRSEIMRPQPEASLAGQQQAQQQAQAMETSAAMVIDMQQGLCQAPGRMPSDLFASSSSSSSSSSSSGNGSIQQSPVYTQTSHLIGGLSASEDMQMQCELFSPTSVPNSDAATPGQPQEQVPAGGPALFPASGCSAEGKEASGQAKQMQSTVFQAMVQMQHSGEGQAQVALFPSSEEMLAAVQGPGAQQQSGGLFQQSGEQMLSLQAGSFLSPAAHSQPQLFHSQSPIGDAQSISQEAPGSLFHSQNAAASSEQLQQPMFHSQGSLGVVPDQQGGSLFLPQNAQEEQMPFFATQSSLSPLQGPARAEQQPSFQQPHLQGPLLAQEQHQPTHQGLFQAQVTLAPLPPSGVPPNQQGGIFQAQHSLVALQTSASAQEQQQQQQQQSLRFSSQNPMSSVASQKPTLLFSSSPSTMASQEQQQQSQPLFHPQRAQEQQQQQQQPMQFQSQGSGSTQAEPPQPPPGLFHGSPQMQLGSPSAQEQPVTLFLSSASMSALQSNMGQQELQQPPLYPSQSSLPNIQDSSSPPQQQQQAASLFHNASAGSSLSQLQNSSASSPQTSGMFLFGIQDTGCGQLLTSGPGSVSEQMMTLSQAQNEVQPSVTALLSQQMSESPQISSAMTTNQNMKKIDDLLVSLQNQGNNAMASSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH48</accession>
    <name>A0A6I9XH48_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Calcium-binding protein 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106539942</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910348.1">
        <property type="nucleotide sequence ID" value="XM_014054873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH48"/>
    <dbReference type="GeneID" id="106539942"/>
    <dbReference type="KEGG" id="tsr:106539942"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051111">
        <property type="entry name" value="Ca-binding_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46311:SF3">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46311">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026669458" evidence="3">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013910348.1"/>
        </source>
    </evidence>
    <sequence length="130" mass="15263" checksum="F21988DEE6092A91" modified="2020-10-07" version="1">MAMASFPSRSWEWPCALWDTCLARWNWPLLCSGWTWMFDMQRITLEELKHILYHAFQDHLTMKDIENIIITEEESLNENSGNCQTEFEVHSQKQNRQTCVRKSLICAFAMAFIISVMLIAANQILRSGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHF9</accession>
    <name>A0A6I9XHF9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kanadaptin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SLC4A1AP</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913302.1">
        <property type="nucleotide sequence ID" value="XM_014057827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHF9"/>
    <dbReference type="GeneID" id="106542176"/>
    <dbReference type="KEGG" id="tsr:106542176"/>
    <dbReference type="CTD" id="22950"/>
    <dbReference type="OrthoDB" id="433755at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd19856">
        <property type="entry name" value="DSRM_Kanadaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22677">
        <property type="entry name" value="FHA_Kanadaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="95"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913302.1"/>
        </source>
    </evidence>
    <sequence length="690" mass="77209" checksum="C09AC2CD06C6D884" modified="2020-10-07" version="1">MAAPTEESIPSEDFKKPNLPPPLPLSLARGKAAEGEAPKRPHGPEVGPCPPVLPDYQEPPWGGCPSEEDSSGYSLETVKGGTVLDRVSLAGRSRLLVGRAPGCDLSLVHPCVSRHHAVLQCRPPRSSAEGEKEPLASESPEPGLYIYDLDSAHGTFLNKARVPPRTYCRVRVGHVLRFGGSSRLFILQGPEEDQEPESELTVTQLKELRKQQKAKLEKTMLGDDSDEEQKEIEIKKSETSRSDLSCSWGIGEDAEEEEGEENPIAVEFQEDQEAFYLKDPKKALQGFFDREGEELEYEFDSHGTGTWLCRVKLPIDDASGKQLVAEAIHSGKKKEAMIQCALEACRILDARGVLRQEAVSRKRKAKNWEEEDFYDSDDDIFLDRTGVIEQKRLNRMKKAGKIDEKPETYDSLVAKLHAGERELSEIAEKLKSSRTTQSHSATQDSLDEFMTEIKSGCSVDSVTRKKLHLRTFELRKEQQRLKGLIKIVTPASMPQLKSQFQNQDLESQNKPKKLTLPLFGAMKGGSKFRLKTGTVGKLPMKRPDIPVNLLKMKDERPEEEEEEEEEEEDDVNSSVNQSSQNTKMEMVKEENPLSSSYSPSNQRLKSQSESSSKHTSSLAHNSPVNELQVKLEASEMPAEKPKNQKDSSKPKIQHSLSSNYPEDDPDYCVWVPPEGQSGDGRTHLNAKYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHK6</accession>
    <name>A0A6I9XHK6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport and Golgi organization protein 6 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TANGO6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913357.1">
        <property type="nucleotide sequence ID" value="XM_014057882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHK6"/>
    <dbReference type="GeneID" id="106542217"/>
    <dbReference type="KEGG" id="tsr:106542217"/>
    <dbReference type="CTD" id="79613"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057407">
        <property type="entry name" value="HEAT_TANGO6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019414">
        <property type="entry name" value="Rtp1_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23565">
        <property type="entry name" value="ARM_TANGO6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10304">
        <property type="entry name" value="RTP1_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TANGO6 HEAT repeat" evidence="6">
        <location>
            <begin position="51"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="5">
        <location>
            <begin position="494"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="4">
        <location>
            <begin position="693"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="458"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913357.1"/>
        </source>
    </evidence>
    <sequence length="746" mass="81429" checksum="CD507FAAE7173BDA" modified="2020-10-07" version="1">MDTCHVNTMSLIPGEQAPKACRRSQQNDEPRTALAPAWLQRLCGHLLSGRLLRPGGVQAVLRGILEEAGAGAVRGSSAEAAAADWQKCEAIAKILSSCPQQSLSLENYCQGICPQILQLFLIQDKLMMRQFQRVATATVLAMVRQHPQQAKEHLLRPLLEPLLRCLGEPEGSLEDLPAGSVLVEEELLSGCVETVYKVCVVGNEPLPCLLGALQPALGAVFSLWCFTKQNVSSLRTPCQEILLWFLEKSEQQASLEALEGLAELRGSPRLPHPHCQFQVGSEGGVVATIREAESDEDEALYRKLSEEQWQLEQLVDLLSQCRRGSLAGDFFLGCLKELSQMASERDPAPPSQGHPLDLEQLCHPALPQQTRQLQVLRLVALLTEGISDSLLTDIRQLKSGDFGSLKQLVPLLEELSRLHPEPVIQELAADLSISICTHRAFSTEAVRTAACQALGGRVGGAAAQKPPGSRPGQPQGTDSVAQGSSAPCGPQSQLQELLSEAYSPDIPTRAAALRLLSQRVERREPGALETREKVLEVFVENLEHKDSFVYLSAIQGLALLSDASPQDILPLLLSQYAGAPLAETRMKVGEALLRTTRALGDLAFQHREKLIHAFLRGVRDPDPSLRASSLSNLGELCQILGFQLGSVVHELASCLAAVVKTDHEAEVRRAGVHVVVLLLRGLSTKAVEVLRDVLLDLYRLLKWVVRCEGDEVTVLHAQLALEELDGLLRQLLFPPQALRKKIEILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK04</accession>
    <name>A0A6I9XK04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RING finger protein 186</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RNF186</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916129.1">
        <property type="nucleotide sequence ID" value="XM_014060654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK04"/>
    <dbReference type="GeneID" id="106544397"/>
    <dbReference type="KEGG" id="tsr:106544397"/>
    <dbReference type="CTD" id="54546"/>
    <dbReference type="OrthoDB" id="6106880at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051435">
        <property type="entry name" value="RING_finger_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="89"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916129.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="28740" checksum="CEECB11F2F2585A6" modified="2020-10-07" version="1">MDDLQLVKEAGKPMDRSEISGETSGTDDGPRIEGCSIQEESPCNKAVDLELRNPDNETSASSHPVAVSVSPSVPLQPCPLIVSATDMTCLICFHQFSLARLPKVLACQHAFCAVCLQVILRQEDHIWIVSCPLCRKATVVFGGLVCSLRNLQHVMERLGAPDPEAEIAVGVWHSQQPSSPEIQSQDSAEINQAAIKRLILLLLLVSVLIVFILPFTETGLLTWFLCFLVVLGGIICAVLCWDPSWSRPSFSLPLWTKKVNQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLI9</accession>
    <name>A0A6I9XLI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ena/VASP-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ena/vasodilator-stimulated phosphoprotein-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EVL</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Stress fiber</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914831.1">
        <property type="nucleotide sequence ID" value="XM_014059356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLI9"/>
    <dbReference type="GeneID" id="106543354"/>
    <dbReference type="KEGG" id="tsr:106543354"/>
    <dbReference type="CTD" id="51466"/>
    <dbReference type="OrthoDB" id="31170at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000004">
        <property type="entry name" value="Enah/Vasp-like, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000071">
        <property type="entry name" value="Enah/Vasp-like, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017354">
        <property type="entry name" value="VASP/EVL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF4">
        <property type="entry name" value="ENA_VASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038010">
        <property type="entry name" value="Vasodilator_Phospo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="WH1" evidence="13">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="146"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013914831.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="45319" checksum="3B25913CBD7D29C2" modified="2020-10-07" version="1">MYAFEEFSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTACNTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQQDGGPTTQRQVQNGPSPDEIEIQRRQAMEQQQQQRQESLERRTSTTGPTLPPGHPSVATGIPTSSGGPPPPPPPPPPPTGTAPPPPPPLPAGGGSSIDDGSMSGLAAALAGAKLRRVQRPEDGSGGSSPSGAAKSDANRTSSGGGGLMEEMNKLLAKRRKAASQSDKPVDKKEEESQNEDASTSPTPVTRGPVQQQNSSDPGKKLWERSNSMEKPVTSLLSRNPSVGRSPESKGPVQFQPSFRMKPANSSNDVAMDTLDFDRMKQEILEEVVKELHKVKEEIIDAIRQELSRISTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLV4</accession>
    <name>A0A6I9XLV4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Dynein intermediate chain 2, axonemal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DNAI2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916829.1">
        <property type="nucleotide sequence ID" value="XM_014061354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLV4"/>
    <dbReference type="GeneID" id="106544941"/>
    <dbReference type="KEGG" id="tsr:106544941"/>
    <dbReference type="CTD" id="64446"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001017">
        <property type="entry name" value="Dynein axonemal intermediate chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000371">
        <property type="entry name" value="dynein intermediate chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916829.1"/>
        </source>
    </evidence>
    <sequence length="597" mass="68827" checksum="05266999D48CB75F" modified="2020-10-07" version="1">MEIVYVYLKKRSEFGRQCSFSDRAAEISVDIQPDPALATNFIEKDPIDTGVQCAGDMSEHEVNTERFEMETRGINHLEGGWPKDVNPLEMEQTIRFRKKVEKDENYINVITQLGTLMDHCIKQNNAINIYEEYFEEEETAAVTDEAPSAKTVNLFRDPNPIKRTATHLSWHPDRSRKLAVAYSNLEFQRSSKDMSYDSYIWDLEVPNKPDMVIQPASPLVCLEYNPKDSHILIGGCYNGQIAYWDTRKGSLPVELTAVELSHRDPVYNAIWLQSKTGTDCFSGSTDGQVLWWDIRKLSEPTETLVMDISRKGILENALGAIALEFEPTMPTKFMVGTEQGIVISCNRKAKTPAEKIVCTFSGHHGPIYSLSRNPFYPKNFLTVGDWTARIWSEESKESCIMWTKYHMAYLTDGCWSTTRPAVFFTTKMDGTLDVWDFLFKQNDPTLNLKVCDEPLFSLRLQDNGRFIGCGSKLGTVTLLEFSSGLCTLQRNEKNLASAMFERETKREKILEARHREMRLKEKTKLEGKDEEVKEDVEEEKPQEVLKRVHKEFFEVIEGELRRRERAQAKLKIEKEREFEDDDSEIFFPKVGILFHML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XN65</accession>
    <name>A0A6I9XN65_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein kinase C-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106541320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912193.1">
        <property type="nucleotide sequence ID" value="XM_014056718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XN65"/>
    <dbReference type="GeneID" id="106541320"/>
    <dbReference type="KEGG" id="tsr:106541320"/>
    <dbReference type="OrthoDB" id="9378522at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15538">
        <property type="entry name" value="PHD_PRKCBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044075">
        <property type="entry name" value="PRKCBP1_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46453:SF3">
        <property type="entry name" value="MYND-TYPE ZINC FINGER-CONTAINING CHROMATIN READER ZMYND8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46453">
        <property type="entry name" value="PROTEIN KINASE C-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="88"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912193.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="14980" checksum="7DCD2E162FD591D0" modified="2020-10-07" version="1">MDISTRSKDPGSAERTAQKRKFPSPPHSSNGHSPEDSSTSPIKKKKKPGLLNSNNKEQSELRHGPFYYMKQPLTTDPVDVVPQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLKLTAEPEGDWFCPECEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNV3</accession>
    <name>A0A6I9XNV3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Heat shock factor protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HSF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917649.1">
        <property type="nucleotide sequence ID" value="XM_014062174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNV3"/>
    <dbReference type="GeneID" id="106545569"/>
    <dbReference type="KEGG" id="tsr:106545569"/>
    <dbReference type="CTD" id="3297"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010542">
        <property type="entry name" value="Vert_HSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF274">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06546">
        <property type="entry name" value="Vert_HS_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="11">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="237"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="238"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="284"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013917649.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="41956" checksum="C15DC428978CD9FF" modified="2020-10-07" version="1">MEPPGAVAGGGGGGSNVPAFLTKLWTLVEDTDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPEKDDTEFQHPHFLRGQEHLLENIKRKVTHVSSIKNEEVKVRQDSMTKLLTDVQLMKGKQESMDSKLIAMKHENEALWREVATLRQKHTQQQKVVNKLIQFLISLVQSNRILGVKRKIPLMLNDGSSPHSMPKYSRQYSLEHLHSPSPYSASPPAYGGPKLYSPDSGPIISDVTELAQSSPSPCPSSSSFEEERSSPVVRIKEEPPSPSCSPSAEEASPLDAPLSPSAFIDSIFREEQPSSTSAPAPPGHGTRAQPQEKCLSVACLDSVGRAPQMSEVTCLFPSPSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR94</accession>
    <name>A0A6I9XR94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Copper transport protein ATOX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Metal transport protein ATX1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ATOX1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Interacts with ATP7B. Interacts with ATP7A. Interacts (via dimer form) with SLC31A1 (via C-terminal domain); this interaction improves ATOX1 stability and controls intracellular Cu(I) levels.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ATX1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918594.1">
        <property type="nucleotide sequence ID" value="XM_014063119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR94"/>
    <dbReference type="GeneID" id="106546281"/>
    <dbReference type="KEGG" id="tsr:106546281"/>
    <dbReference type="CTD" id="475"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000008">
        <property type="entry name" value="Copper transport protein ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051881">
        <property type="entry name" value="Copper_transport_ATOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365:SF1">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HMA" evidence="12">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013918594.1"/>
        </source>
    </evidence>
    <sequence length="68" mass="7615" checksum="E3B7D62FE5C699E0" modified="2020-10-07" version="1">MPKHEFFVDMTCEGCLNAVTRVLNKLGDVQFEIDLPNKKVSIDSTHSVDTLLDTLKKTGKNTSYLGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRH3</accession>
    <name>A0A6I9XRH3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">SLC44A1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916366.1">
        <property type="nucleotide sequence ID" value="XM_014060891.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916367.1">
        <property type="nucleotide sequence ID" value="XM_014060892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRH3"/>
    <dbReference type="GeneID" id="106544580"/>
    <dbReference type="KEGG" id="tsr:106544580"/>
    <dbReference type="CTD" id="23446"/>
    <dbReference type="OrthoDB" id="420519at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015220">
        <property type="term" value="F:choline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF56">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="630"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="630"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013916367.1"/>
        </source>
    </evidence>
    <sequence length="653" mass="72668" checksum="B000DF8BB72D4632" modified="2020-10-07" version="1">MGCCGSAASQSSKREWKPLEDRSCTDIPWLLLFILFCIGMGFICGFSIATGAAARLISGYDSYGNICGQKNVKIEGIGNSGLDLTEKKFVFFLEPCNVDLVRRKIKSIALCVSACPRSELKTLDDVQKFADINGSALCSYELKPSEYTTHVNAAKLCPKLPVPESAPIPFFHRCAPVNISCYTKFAEALITFVSDSSILHRLISGVMTCKEIILGLCLLSLVLSMILMVIIRYISRVLVWILTIVVVLGSLGGTGVLWWLYVKMSPNGTLTVEQLQIAKDNRQALLIYAIAATIFTVILLLIMLVMRKRVALTIALFHVAGKVFIHLPLLVFQPFWTFLALILFWIYWIMVLLFLGITGNPVANEEGLVEFRITGPLKYMWWYHVVGLVWISEFILACQQMTVAGAVVTYYFTREKRNLPFTPILASVNRLICYHLGTVAKGSFIITLVKIPRMILMYIHSQVKGKENVCARCILKACICCLWCLEKCLTYLNQNAYTATAINSTNFCTSAKDAFVILVENALRVAAINTVGDFMLFLGKVLIVCSTGLAGIMLLNYQRDYTVWVLPLIIVCLFAFLVAHCFLSIYEMVVDVLFLCFAIDTKYNDGSPGKEFYMDKVLMEFVENSRKALKEAGRGNGGEGKELKPMVSGASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS34</accession>
    <name>A0A6I9XS34_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein goosecoid</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GSC</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916561.1">
        <property type="nucleotide sequence ID" value="XM_014061086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XS34"/>
    <dbReference type="GeneID" id="106544728"/>
    <dbReference type="KEGG" id="tsr:106544728"/>
    <dbReference type="CTD" id="145258"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000210">
        <property type="entry name" value="homeobox protein goosecoid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051440">
        <property type="entry name" value="Goosecoid-like_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="166"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="168"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="221"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013916561.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="28985" checksum="AC8A4AF3F14C0F48" modified="2020-10-07" version="1">MPAGMFSIDSILAARPRGKESGLLLQQHQPPHPAQQPQPSAAPVVFSASLHGGDTIYGPAGGDYGGYYSRAVAPASNLAALGGSRLGGYGNYYYGQLHVQASAVGPSCCGAVQPFGAQQCSCVPATGYEGTGSVLMSPVPHQILPYMNVGTLSRTELQLLNQLHCRRKRRHRTIFTDEQLEALENLFQETKYPDVGTREQLARRVHLREEKVEVWFKNRRAKWRRQKRSSSEESENAQKWNKTSKASPEKLQEESKSDLDSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSV6</accession>
    <name>A0A6I9XSV6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gamma-aminobutyric acid receptor subunit alpha-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">GABA(A) receptor subunit alpha-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">GABRA1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Postsynaptic cell membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916821.1">
        <property type="nucleotide sequence ID" value="XM_014061346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSV6"/>
    <dbReference type="GeneID" id="106544906"/>
    <dbReference type="KEGG" id="tsr:106544906"/>
    <dbReference type="CTD" id="2554"/>
    <dbReference type="OrthoDB" id="203862at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19034">
        <property type="entry name" value="LGIC_ECD_GABAAR_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19052">
        <property type="entry name" value="LGIC_TM_GABAAR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000001">
        <property type="entry name" value="Gamma-aminobutyric acid A receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000002">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit alpha-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005431">
        <property type="entry name" value="GABBAa1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047024">
        <property type="entry name" value="Gabra-1-6_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047079">
        <property type="entry name" value="GABRA1_ECD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01614">
        <property type="entry name" value="GABAARALPHA1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0868">Chloride</keyword>
    <keyword evidence="13" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 26" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 22" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027148183" description="Gamma-aminobutyric acid receptor subunit alpha-1" evidence="22">
        <location>
            <begin position="26"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="23">
        <location>
            <begin position="41"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="24">
        <location>
            <begin position="255"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00033008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013916821.1"/>
        </source>
    </evidence>
    <sequence length="455" mass="51884" checksum="ECA8E43F018162A6" modified="2020-10-07" version="1">MMHLLYDHFWAWVLLMSTLPGKSYGQTSLQDEIKDNTTIFTRILDRLLDGYDNRLRPGLGERVTAVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMQVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTKSEVMYEWTREPARSVVVAEDGSRLNQYDLMGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIMKNNSFTAPAPATSFTPNIARDPGLATIAKSATIEPKDVKAETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKDKNPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTE4</accession>
    <name>A0A6I9XTE4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Integrin alpha-X-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106545083</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917021.1">
        <property type="nucleotide sequence ID" value="XM_014061546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTE4"/>
    <dbReference type="GeneID" id="106545083"/>
    <dbReference type="KEGG" id="tsr:106545083"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000012">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048633">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF118">
        <property type="entry name" value="INTEGRIN ALPHA-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00357">
        <property type="entry name" value="Integrin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21520">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1130"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="42"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="105"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="177"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="421"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="477"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="538"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="600"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1170"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1178"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013917021.1"/>
        </source>
    </evidence>
    <sequence length="1189" mass="131537" checksum="332797564F06D38E" modified="2020-10-07" version="1">MDGMCQFCLLELQRGHDKTFADSSYFLNVVIHNMLTPCHGFSVDTEQPVIFRERVKSFGYSVVQFGSGTNGGVLVGAPLQQGGVNETGNLYKCQGTSSKTGGCQEMDLQRPADAVNMSLGLSVSAHDSQVLVCGPRVHQTCGEYIYLKGYCFLLDQNLRQIQRFPDKLPECARRPADIVFLIDGSGSISTRQFSQMKTFISEVIKRFQNTNTQFGLSQYSGSYEEHFDFLLFKTTPNPDDLIKTVRQLHGATHTATYIQRVVREMFLPEKGARSDASKVLIVITDGEKSGDRLEYSDVIPEAQRANIIRFAIGVGRAFSRGNARQELMTIASEPAMNYVFAVNNFDALKDIQNSLQSKIFAIEGTQSQNTRNSSQSFELELSQEGFSALHTQSGTLLGAVGAYDWAGGFFLFNSQDDATFINISSSTSDLKSAYLGYSIQEVRLNGQSSFVVGAPRYQHVGKVVWFSQRRRQWKPKSELSLGQSPQIGSYFGASLCAVDLNRDGNTDLILIGAPMYYDGIAGGKVYVCERRGETFSCNVELQGEPQHPLGRFGTSITEAGEITGDRWTDVAVGAPLEDENQGAVYIFQGTSSSLNPVFSQRIPASAFASRLQYFGQAIDGGSEITGDGLPDLAVGADGQVLLLRARPVVNVVVGITFNPTMIPISAFECGDQQMLNKEISTATVNFKIQEDSRFNFGSDILSTIRYTLTLDPGRQKVRAVFNQGSELSTLTEEMKIGVRPESRQYRIKLPTCIEDSLTPIILQLSYTLIGEPIQRANNVRAILQKDAPVLIPASLPFEKDCGKDGICKDQLKTSFNFSGLNELVVGLTLDLNVTVFLRNDGEDSYSTNLTFIYPSSLSYRRYILVQSNRKYVVIKCTPFPAAEGSVRNTTCGINQPIFRSMAEVIFIVSFFVSQDANLGSKVQISTTATSENTVAVTKDTFHQMELPVKYAIYVFINSLDESTKYVDFSAGQEDEIRTVEHRYELKNTYHRNASVSVTFHIPLSLNGIQIWNPVFDVPKEFPQIICAPETITPGSKEFVKQLRKQPVLNCSVATCKTIICNVSSLERHQPIEFKIKGNIGLKWLSQTQQKKVTLGSSAQVSYDESKYKQKEGFVWVQAATIVEFLEPYNYLPIIIGSSVGGLVLLVLIIAGLYKLGFFKRQYKQMIEAADNEVAESPPETSTPSDATKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTM2</accession>
    <name>A0A6I9XTM2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ras association domain-containing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RASSF10</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917377.1">
        <property type="nucleotide sequence ID" value="XM_014061902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTM2"/>
    <dbReference type="GeneID" id="106545376"/>
    <dbReference type="KEGG" id="tsr:106545376"/>
    <dbReference type="CTD" id="644943"/>
    <dbReference type="OrthoDB" id="10034447at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16132">
        <property type="entry name" value="RA_RASSF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033593">
        <property type="entry name" value="N-RASSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033634">
        <property type="entry name" value="RASSF10_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286:SF13">
        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="3">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="414"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013917377.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="56229" checksum="14111F4819043F08" modified="2020-10-07" version="1">MEPEEKKISVWICQEEKLISGLTKRTTCSDVVQVLLEESKQRRRRRLQQQQQASLQECGGGMLSGLPQSYCIVEKWRGFERILPNKTKILRLWAAWGDEQENVRFVLVRSEASLPNTGPRSAEARVVPSRDSPGCHGLGVTARASLALSQERQRRVVRKAFRKLAKINKRKGQPQLEEPPAKEAPAGERMETLVHLVLSQDHTIRQQIKRLRDLDREIDRYEARIHFDRMKRHGVNYVQDTYLVGEEPEPLAGEQELKSPSGTAPIAASTAAGELEQPSSGSEDLEEYAWKCEQVARVQEQWRRQEELIEHLAGEIQEELNERWMRRRREELAAAKGSSTSLSETDCNTTELSGGGGELHVEQERIKTQLSTSLYIGLRLNTDLDAVKSDLEFTQRAWGDKERELQRLLDSLESLHVEGEGEEEEETAVADERRDLPFSPGEELPASVPITSGNWGEEAARSLSKDCEDREDEDSDTGLSSMHSQDSDSVPVCESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XU28</accession>
    <name>A0A6I9XU28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RAB8B</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle membrane</location>
            <topology evidence="13">Lipid-anchor</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013914193.1">
        <property type="nucleotide sequence ID" value="XM_014058718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XU28"/>
    <dbReference type="GeneID" id="106542875"/>
    <dbReference type="KEGG" id="tsr:106542875"/>
    <dbReference type="CTD" id="51762"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000202">
        <property type="entry name" value="ras-related protein Rab-8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013914193.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="23068" checksum="E75B7B7A5DC89AF4" modified="2020-10-07" version="1">YDYLFKLLLIGDSGVGKTCALFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNWIRNIEEHASSDVERMILGNKSDMNDKRQVSKEKGEKLAIDYGIKFLETSAKSSINVEEAFFTLARDIMTKLNKKMNDSISAGASGPVKITESRSKKSSFFRCSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV52</accession>
    <name>A0A6I9XV52_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LRRN4 C-terminal-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106545578</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917666.1">
        <property type="nucleotide sequence ID" value="XM_014062191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV52"/>
    <dbReference type="GeneID" id="106545578"/>
    <dbReference type="KEGG" id="tsr:106545578"/>
    <dbReference type="OrthoDB" id="8824963at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="114"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013917666.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="30188" checksum="42103EC6E725D7EC" modified="2020-10-07" version="1">MACGLLMLCPRGSPTSMGHPLPKPLQGPYTPHPSFSHPPLFGPDKASPAASDDYDYDEDYYDNFQSTESPRFLSPGPPTPFCAYDRCKHLQVPCGELSRLSKCLCPGVTGPDITPGAPRLRAVHVSETRASLHWCAPSSTVHVYHLKYCRPGEDFVLGPPLNSTVRVAALYDLVPGTEYLLCVVAANQAGFSPTDDGVREHGPCRQVRTPAHQMTYVYIAVGLGCVLILGVVSVLLWHFYTRKAKPVQHGSLDNILDGELSSGHAGATNSSFRTEEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVK8</accession>
    <name>A0A6I9XVK8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neural cell adhesion molecule L1-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106545715</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917831.1">
        <property type="nucleotide sequence ID" value="XM_014062356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVK8"/>
    <dbReference type="GeneID" id="106545715"/>
    <dbReference type="KEGG" id="tsr:106545715"/>
    <dbReference type="OrthoDB" id="6244967at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000367">
        <property type="entry name" value="Neural cell adhesion molecule L1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000418">
        <property type="entry name" value="Neural cell adhesion molecule L1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000768">
        <property type="entry name" value="Neural cell adhesion molecule L1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="NEURAL CELL ADHESION MOLECULE L1-LIKE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1"/>
            <end position="79"/>
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    <feature type="domain" description="Fibronectin type-III" evidence="8">
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            <begin position="84"/>
            <end position="186"/>
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    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="187"/>
            <end position="287"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008588"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013917831.1"/>
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    </evidence>
    <sequence length="480" mass="53490" checksum="1DAC5AB4263D8DAD" modified="2020-10-07" version="1">MNMTWEPLKPMEHNGPGLEYKVNWKQQDTNEWEEETVQKHFLVLQNTPTFVPYDIKVQAVNKYGSGPEPNILTGYSSEDIPEAAPSNVIVQVLNSSLAKVIWSTIPKDRVRGHLRGYKVNWWKIRNRLDEKNHHPEKHSLTFKADRNDGMVPGLEPFSIYHLTVLAFNSKGNGPESIAYEFQTPEGVPEKPHFLKLLNFEKDSVTLSWGPPKKPNGFITGYTLQYQMINETDNIGPLHEISITNSSSLSFRLSNLSLSTKYKFYARACTVQGCGKPITEEGVTVNHGSKAVGKITEVKPTPKSQPVEALDPGTANTIRLMTKNWVDNNSISADVIETRGRAYTGIYERISTQGWFIGLMGAVALLTLLLLTICFVKRNKGGKYSVKEKEDLHPDPEGQSIKDEIFGEYSDSDEKPLKGSLRSLNMDIKGAESADSLVDYGEGDHRMFNEDGSFIGAYAGCKEKASVESIGSSTATFPLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW19</accession>
    <name>A0A6I9XW19_9SAUR</name>
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        <submittedName>
            <fullName evidence="3">Zinc finger protein ZFPM2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106546081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_014062857.1"/>
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        <property type="component" value="Unplaced"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:heart development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="FOG"/>
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    <dbReference type="PANTHER" id="PTHR12958">
        <property type="entry name" value="FRIEND OF GATA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12958:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN ZFPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
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            <begin position="18"/>
            <end position="33"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="70"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="80"/>
            <end position="94"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013918332.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="13778" checksum="030E3A23FCE1A58D" modified="2020-10-07" version="1">MSRRKQSKPRQIKRPLEDAVEDEEEECLSEENEIISKEDFPLEGNFSAEFESGNLNCEDVEYFCNKGDEEGSQETADSDGDARSDKPGPLKLETEDWDGPGELEMFQRDGERKIQSRQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW75</accession>
    <name>A0A6I9XW75_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Scm-like with four MBT domains protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">SFMBT1</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918396.1">
        <property type="nucleotide sequence ID" value="XM_014062921.1"/>
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    <dbReference type="RefSeq" id="XP_013918397.1">
        <property type="nucleotide sequence ID" value="XM_014062922.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:histone binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="MBT_SFMBT1_rpt1"/>
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        <property type="entry name" value="SAM_Scm-like-4MBT1_2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.190">
        <property type="entry name" value="SLED domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004092">
        <property type="entry name" value="Mbt"/>
    </dbReference>
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        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
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    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
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    <dbReference type="InterPro" id="IPR037604">
        <property type="entry name" value="Scm-like-4MBT1/2_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021987">
        <property type="entry name" value="SLED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038348">
        <property type="entry name" value="SLED_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF77">
        <property type="entry name" value="SCM-LIKE WITH FOUR MBT DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02820">
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    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
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    </dbReference>
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        <property type="entry name" value="SLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00561">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51079">
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <feature type="repeat" description="MBT" evidence="5">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="5">
        <location>
            <begin position="128"/>
            <end position="232"/>
        </location>
    </feature>
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        <location>
            <begin position="242"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="5">
        <location>
            <begin position="357"/>
            <end position="454"/>
        </location>
    </feature>
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            <begin position="789"/>
            <end position="855"/>
        </location>
    </feature>
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        <location>
            <begin position="643"/>
            <end position="754"/>
        </location>
    </feature>
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        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="684"/>
            <end position="697"/>
        </location>
    </feature>
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        <location>
            <begin position="699"/>
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        </location>
    </feature>
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        <location>
            <begin position="717"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="733"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918397.1"/>
        </source>
    </evidence>
    <sequence length="862" mass="97432" checksum="3CA147E22D162789" modified="2020-10-07" version="1">MNGEQQRETDAGSVVEEAEFSWDDYLEDTGATAAPHSSFRHVDTSPQNGFTPGMKLEVAIKPGPDTYWIATIITTCGQLLLLRYDGYGEDRKADFWCDIMTADLHPLGWCEQNKKTLKVPEGIKDKIPNQIEFLQQILKGACSPPAGLLEGLHRGKNSLDLIAPGSRLECQHINSCLGAWIVTVLENIGGRLRLRYEGLGDVEQYDLWMFYLDPFLHPMGWVSQQGYILEPPLAIRHLKTEIEWQETLAKVKEEEEESLVPTDLFKDKPVVGTHAFSVNMKLEAINPKAPFAISPATVTKVFDDKYFLVEIDDLRPDCTTRQPFICHVNSAGLFPVQWSLKNGLHLNPPPGYPGQDFDWADYLKQCGAEAAPQSCFPLFNSEHGFKENMKLEAVNSENPEEICVATITKVKDSYLWLQLESSKIPVPEFIVSVESMNIFPVGWCETNGYQLRPPRRVVVNKEKKIAVVQPEKQILSSRTVSEGLKTQELNSADTIVTNGKYCCPKIYFNHRCFSGPYLNKGRIAELPQSVGPGNCVLVLKEVLSLLINAAYKPSRVLREIQLDEEASWHGYGEILKAKYKGKSYRATVEVVRTADRVADFCRQTCIKLECCPNLFGPQMVLDKCSENCSVLTKTKYTHYYGKRRNKRIGRPPSIHSNIEASMKKANRKRKKRKNFFVHKKKRSSTSVDNTPVGSPQGSGDEDDQDEIDEESLTEDSTSEHQEELSEVSEKKSQSSSPTQSEQSHFGAPDRDKRKRKLRTFSFSDDENRPPSPKEIKIEVDEKLQLDSNPLEWSVADVVRFLKSTDCAPLARIFLDQEIDGQALLLLTLPTVQECMDLKLGPAIKLCHHIERVKFAFYQQFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXR2</accession>
    <name>A0A6I9XXR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA guanylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">RNGTT</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.7.50" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013909265.1">
        <property type="nucleotide sequence ID" value="XM_014053790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXR2"/>
    <dbReference type="GeneID" id="106539105"/>
    <dbReference type="KEGG" id="tsr:106539105"/>
    <dbReference type="CTD" id="8732"/>
    <dbReference type="OrthoDB" id="200924at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17664">
        <property type="entry name" value="Mce1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000111">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.430:FF:000001">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000040">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.87.10:FF:000001">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.87.10">
        <property type="entry name" value="mRNA Capping Enzyme, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017074">
        <property type="entry name" value="mRNA_cap_enz_bifunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036958">
        <property type="entry name" value="mRNA_capping_HCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="104"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="436"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="13">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="322"/>
            <end position="324"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="392"/>
            <end position="397"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036958-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013909265.1"/>
        </source>
    </evidence>
    <sequence length="461" mass="52636" checksum="063D9F691479359D" modified="2020-10-07" version="1">MSFNKIPPRWLNCPRRGQPVAGIFLPLKTMLGPKYDDKVAEEFRFHPSMLSNYLKSLKVKMGLLIDLTNTSRFYDRSEVEKDGITYVKLQCKGHGECPTPENTETFIRICEQFRNKNPTELIGVHCTHGFNRTGFLICAFLVGKLDWSIEASVATFAQARPPGIYKGDYLKELFRRYGDVADAPAPPSLPEWCFEEDEEDGEEDNCKLGTPESEACSSTSFSGRRKKEHLKLAQPVGECDFNIRLSCIEKEIILPRHDKMKNGQIDKAQEPFSVRNKPFFDINIAKKLLEGNFAREVSHEVDGLIFQPVGKYKPGRCDDILKWKPPSLNSVDFRLKLARVVGEGLLPRNVGLLYVGSLDRPFAEIKVTKDLKQYDNKIIECKFENNSWVFMRQRIDKSFPNAYSTAMAVCNSIQHPVTKEFLFEFIEKCMTAAPGQPRKHHLDPDTELMPPPPPKRPRLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYK5</accession>
    <name>A0A6I9XYK5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA-binding protein inhibitor ID-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ID3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918896.1">
        <property type="nucleotide sequence ID" value="XM_014063421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYK5"/>
    <dbReference type="GeneID" id="106546535"/>
    <dbReference type="KEGG" id="tsr:106546535"/>
    <dbReference type="CTD" id="3399"/>
    <dbReference type="OrthoDB" id="10047910at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19693">
        <property type="entry name" value="bHLH_dnHLH_ID3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000039">
        <property type="entry name" value="DNA-binding protein inhibitor ID-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026052">
        <property type="entry name" value="DNA-bd_prot-inh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11723">
        <property type="entry name" value="DNA-BINDING PROTEIN INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11723:SF16">
        <property type="entry name" value="DNA-BINDING PROTEIN INHIBITOR ID-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="32"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918896.1"/>
        </source>
    </evidence>
    <sequence length="127" mass="13971" checksum="33ABF255FF133A07" modified="2020-10-07" version="1">MKAISPVRSVRSCYEAACCVSDQSLAIARGSSNKSPALEEPMSILYDMNDCYSKLRELVPGIPQGSKVSQVEILQHVIDYIFDLQIVLEEQAKKGQDPSTEASLLSLKAAELASELCSKEERTLCHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ48</accession>
    <name>A0A6I9XZ48_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein Nkx-2.8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NKX2-8</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NK-2 homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909770.1">
        <property type="nucleotide sequence ID" value="XM_014054295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ48"/>
    <dbReference type="GeneID" id="106539493"/>
    <dbReference type="KEGG" id="tsr:106539493"/>
    <dbReference type="CTD" id="26257"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000101">
        <property type="entry name" value="NK2 homeobox 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF118">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX-2.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="83"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="85"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909770.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="24853" checksum="03CB0546E38A2B60" modified="2020-10-07" version="1">MATLARGSFTVRSLLDLPEQEGGGGGGSEGQEPAESYAASPFRAWMETERSHYLSSDDSGAESRPRPAAASTRQAAREEDEEKLKKKRRVLFSKAQTLALERRFRQQRYLSAPEREQLAHLLRLTPTQVKIWFQNHRYKMKRAKGGERGGAGSGPPLMRRVVVPVLVRDGKACQSCTGAAPSRSPCGPQAAFNLPGYSPFPQTSSLGLFPAYQHLAPPSLVSWNWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZT8</accession>
    <name>A0A6I9XZT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">TRPM6</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013910030.1">
        <property type="nucleotide sequence ID" value="XM_014054555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539701"/>
    <dbReference type="KEGG" id="tsr:106539701"/>
    <dbReference type="CTD" id="140803"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000129">
        <property type="entry name" value="Transient receptor potential cation channel, subfamily M, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1010">
        <property type="entry name" value="TRPM, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
        <property type="entry name" value="TRPM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041491">
        <property type="entry name" value="TRPM_SLOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032415">
        <property type="entry name" value="TRPM_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037162">
        <property type="entry name" value="TRPM_tetra_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF15">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY M MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18139">
        <property type="entry name" value="LSDAT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25508">
        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16519">
        <property type="entry name" value="TRPM_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="8" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="18 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="830"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="897"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="924"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="963"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="1039"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="1083"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="29">
        <location>
            <begin position="1742"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1454"/>
            <end position="1477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00025760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013910030.1"/>
        </source>
    </evidence>
    <sequence length="2013" mass="231926" checksum="53287E0B2C2DE514" modified="2020-10-07" version="1">MCKNCTQLKRFSQMFSTFTLPSVQWITVQRFCRMSSMPESNSSEKEKYNVEWSVEKDTKMSPTDAFGTINFQDSDHTYHAKYIRTSYDIKLDQLLQLMIKEWRMEFPKLVISVHGGIQNFKLPSKVVQLFSKGLVKAAETTGAWILTEGINTGISKHVGNALKFHASQYLRKVCAIGISPWGIIENQKELIGKDVVCLYQTLGNPLSKLSTLNSMHSHFILVDDGTMGKYGSEMKLRRSLEKYLSLQKIHTRMGQGVPLVGLLVEGGPNEILTVWEYVRDTPAVPVVICEGTGRAADLLAFTYKHTTDDTNWNAQLKEKILLKIQNSFSFGQKQSHHLLKILMECMEHRDSITIFDAESEEHQDMDLAILTALLKGTHMSAPDQLNLALAWNRLDIAKKHILVYGQHWKVGSLEQAMQDALIMDRVEFVKLLIEHGVNLHRFLTISRLEELYNTKQGPTNLLLHHLLCDVKQSTFPVDYKISLIDIGLVIEYLIGGAYQSSYTRKHFRILCNNIYRKHKNQEVVSRLAQNVSLHQVNQTGSRSGTAENTLHSQFIRTAQPYKYKEKSPNLHKSKKKVKDEFTVFDDQESVGFIYPYNDLLVWAVLMKRQKMAMFFWQHGKEAMVKAVVACKLYRAMAREAKQCNMEDDTAEELKKYSNEFGQLALDVLDNAFKQNEQMAMKLLTYELKNWSNSTCLKLAVSVGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWCKVILSILLPPTILMLDFKSKAEMSHVPQSQEPHQFTWYHGDPNTHSGKDNLSLNNYDFEKEVEKQAEKPTFPNNNEQQSLLGTRKIYEFYNAPIVKFWFHTMAYIAFLMLFTYTILVKMKPTPSAQEWLVITYIFTTFIEKAREIFISEPSKFIQKVKVWIYEYWNFTDFIAIILFMTGFGLRWANPPLQTVGRLIYCLDIIFWYARLLDFFAVNQHAGPYLTMIGKMTANMFYIVIMMAIVLLSFGVARKAILSPNEQPSWTLARDIVFQPYWMIFGEVYAGEIDACELDKNCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYFDMKSMSNKLWKYNRYRYIMTYHEKPWLPPPFIILSHIGLVLNCLCNRRLPSQLDQEEKEHVELKLYLSDEELKKLHDFEEQCVEQYLHEKKESFHFSDSEQIRVTNERVEEMFSQLKEIHDKVIYIKDSLLALDSQLGHLQDLSVLTVDTLKVISAVDTLQEGEVFLSEQKHQTCRKLPHSWSNTLCSKALSCMESENIKYNYYSMPPSLQRNLARSHWSLGGDHPILDGLNYGEIQLVKKEQEQEVENGLLTSELSSENKSGSRYGQFLLVPPDQKGASEDAGLSLALSTTLNELKDEDLKDEIKQNDTVPQCHVCSANDVAKAMPVISQMQELNISLAKKESETTAEHHLSIHAVNSISEISQSAPYHCEGPGKGYVNWGFLEDNEKRDGGNWSRRACQQSPCGKEDGRCSPPHMQIKESKSFSYSSDVSEYSSDNSQSRLTFQRSMSVWINPLKRNWPFCKGSSFCAHGAEKTAKTCKIKEVSKSTQLYQSVWAKAKFGNKERRSSRKKRKTLQVPLITVDSCPQSIQVNFEPLHSKSAEDEEERRAKWLTVSHFKQLSLDHISCMHQEMKNQELVRQTVQICDYLRQSQEDLNNSIAWTRKNRKNNLSRSYSLRSLDGDGNISLSLKTPQDLHHHYSAVERNNLMRLAQTIPFTPVQFFAGEEVTIYRLEESSPSSLDKSMSSWSQHGMAAMIQVLSQEEMDGGLRRAMKAVCTWSENNVLKLGEVFIVKSFLPEVVRTWQKIFHESTVLHLCLREIQQQRAAQKLIYTFNQMKPHSIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPSNLLEEVMLAFSHWSYEYTRGELLVLDLQGVGESLTDPSVIKPEDKQGMTFGPANLGEEAFKNFISKHCCNSCCKKLKLPDLKRNDYASGRVNPAFEIETEIPMAAEDENANNRDDEPLEYYTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y064</accession>
    <name>A0A6I9Y064_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine N-acyltransferase-like protein</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106547122</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013919651.1">
        <property type="nucleotide sequence ID" value="XM_014064176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y064"/>
    <dbReference type="GeneID" id="106547122"/>
    <dbReference type="KEGG" id="tsr:106547122"/>
    <dbReference type="OrthoDB" id="61870at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047961">
        <property type="term" value="F:glycine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010313">
        <property type="entry name" value="Glycine_N-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013652">
        <property type="entry name" value="Glycine_N-acyltransferase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015938">
        <property type="entry name" value="Glycine_N-acyltransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298:SF1">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298">
        <property type="entry name" value="L-COA N-ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08444">
        <property type="entry name" value="Gly_acyl_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06021">
        <property type="entry name" value="Gly_acyl_tr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="151"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013919651.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="33166" checksum="E5A3C0EF88511C7D" modified="2020-10-07" version="1">MLILTCSSKLQLLERVLRKGLPETMLVCGAVMHINRGNPAQHEVVVDSWPEFKAVLTRPQKEVVKDNRDYYANLHAAFYREEDACRTLLENKDAVDWDKAFQLQGLQDGLYQAVKVMAEARSVHMEPHFYQAMLNPDAATLCQNQLRSDPLHLGTLNPSHAALLNDTYEFGGNDRSLRYLRSLIEDFPNACLLDQKGQLVAWSLSDALGCLTHGYALPEFRGKGYMKTVMQALARKLHTKDFALYTRVRSENEISKQHLRGQGFHLLPWTKYVLFFHSKSHETAGAAFKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0X3</accession>
    <name>A0A6I9Y0X3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Platelet-derived growth factor receptor alpha-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106547777</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920507.1">
        <property type="nucleotide sequence ID" value="XM_014065032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0X3"/>
    <dbReference type="GeneID" id="106547777"/>
    <dbReference type="KEGG" id="tsr:106547777"/>
    <dbReference type="OrthoDB" id="9936425at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000720">
        <property type="entry name" value="Platelet-derived growth factor receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000223">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042495">
        <property type="entry name" value="PDGFRL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15360">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR RECEPTOR LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15360:SF4">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25305">
        <property type="entry name" value="Ig_PDGFR_d4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013920507.1"/>
        </source>
    </evidence>
    <sequence length="440" mass="49774" checksum="9D1197AB562AB4ED" modified="2020-10-07" version="1">MGKSGKFEYGFGIFGSTTGLTHIIVQHMEWRLEIELLMHVFIPPTQLMLSISFSTDPAVPFVPSSPEDLFIVVSEEDNSSIIPCRTTDPNTQVTLMNKEGKILYASYDNRLGFIGNFSLGSYICKTTVKAVEFQSGEFFIYVYKGNKNKGIITLDDIKNPTQKLIYNLTIPDASVKDSGDYECAVRHVTKDFKKMERIAIYVHDKGFIDLKPLFKPTEALNLHEVKNFVIYVDAYPEPKLLWLKDNVTLMENLTEIVTSSKQINETREYKQNNPPVSISEASSINNVPALIRDLIDNHHGSSGQSVKCLAEGTPLPDVEWLVCKDIKKCNNDTSWTLLTNNISDVTVETHLDERNMVASQVTFQKVEEIMAVRCMAKNDLRTVTRELKLVAPTLRETGGPEQHRCDDSRETSISTLYSPTKYEGKDLQNGVCLWNLNFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1J7</accession>
    <name>A0A6I9Y1J7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">NADPH oxidase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NOX5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910660.1">
        <property type="nucleotide sequence ID" value="XM_014055185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1J7"/>
    <dbReference type="GeneID" id="106540149"/>
    <dbReference type="KEGG" id="tsr:106540149"/>
    <dbReference type="CTD" id="79400"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000056">
        <property type="entry name" value="NADPH oxidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF58">
        <property type="entry name" value="NADPH OXIDASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="13">
        <location>
            <begin position="327"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013910660.1"/>
        </source>
    </evidence>
    <sequence length="584" mass="67389" checksum="F04DD7DC3D383CFE" modified="2020-10-07" version="1">MNLEDNTKWLMWVAKQFENIAGDNKEINLEQFKTALKVKESFFGDRFFALFDSDESGTISLDELLNTLKLLIHGSETDKLRFLFQVYDVDDQYREPGHRGSSAASWLKPPTAPRKSQIPHILSPVYWYNNKNKLLLLGSYACVNIILFTLAALKQAGSGLWIVVARGCGQCLNFNCAFILVLMLRRSLTWLRTTWIAKVLPLDLNVILHQLMGYIVGALTLLHMGAHIINFVRLSQAKGGYRLWEYLFTTRPGIGWIHGTASLTGLLLQLLTSLMLVCSTTLVRRSGHFEVFYWTHLFYIPIWVLLIVHGANFWKWFVIPGSLFLGEKAFAATLSRVGGLYIVEVNLLPSKVTHLVIQRPPFLHYKPGDYVYLNIPVIARYEWHPFTISSAPEQPDTIWLHIRSLGQWTYRLHEYFWNFQEPLEDNPKLHRMRKEASCSHSNSLDENMKLQKVDFIWINRDQKSFEWFVSLLTQLEAMQGSEEPTACFLEMHLYMTSALNKSDMKAVGLQMALDLLAKKEKKDSITGLRTRTKPGRPDWKQLFQKIDKEDKGKVQVFFCGSPALAKVVKAQCEQFTFPFFRENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y219</accession>
    <name>A0A6I9Y219_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cyclin-dependent kinase-like 3</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CDKL3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013921047.1">
        <property type="nucleotide sequence ID" value="XM_014065572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y219"/>
    <dbReference type="GeneID" id="106548232"/>
    <dbReference type="KEGG" id="tsr:106548232"/>
    <dbReference type="CTD" id="51265"/>
    <dbReference type="OrthoDB" id="548217at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097484">
        <property type="term" value="P:dendrite extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030517">
        <property type="term" value="P:negative regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050775">
        <property type="term" value="P:positive regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000049">
        <property type="entry name" value="cyclin-dependent kinase-like 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000370">
        <property type="entry name" value="cyclin-dependent kinase-like 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF177">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013921047.1"/>
        </source>
    </evidence>
    <sequence length="550" mass="63172" checksum="517E411DE7B5F7AD" modified="2020-10-07" version="1">MEMYEILGKVGEGSYGTVMKCKHKETGHIVAIKIFYEKTEKSVNKIAMREIKFLKQFRHENLVNLIEVFRQKKKIHLVFEFIDHTVLDELQHYSHGLDSRRLKRYLYQILRAIDYLHSNNVIHRDIKPENILVSQTGITKLCDFGFARTLAAPGDIYTDYVATRWYRAPELVLRDTTYGKPVDIWALGCMIIEMATGNPYLPSSSDLDLLHKIVTKVGNLTPHFQSIFIRSPVFSGMVLPEVQHPKSARKKYPKLSALLADMVHACLQMDPADRISSADLLQHEYFTRDGFVEKFLPELKSKLLQEAKLNYLSKFRDNNKEMEQIKDEKRIVHINMSQNGPVTVKEMEKDRKSKEMKIKGIKVKGGKSEISELKRNEQEDAVSQWVTLSQNALNLPYENKPTIGDGTSLITVGIDAGGMKDEAPQTPCTTMPRIHPNCGNLTANFSSHFLCPNSRLQEKAKKRRSPWPSVGQIGSNSRQEDGVLTQNQMEKVSLYERAAQIDQMVNINRKKRNISRCEKREIHLPELNTTGQQKELKAMESMFVIFYRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y312</accession>
    <name>A0A6I9Y312_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prostaglandin E2 receptor EP3 subtype</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Prostanoid EP3 receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Prostanoid TP receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Thromboxane A2 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PTGER3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Interacts (via C-terminus) with MKLN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917573.1">
        <property type="nucleotide sequence ID" value="XM_014062098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y312"/>
    <dbReference type="GeneID" id="106545503"/>
    <dbReference type="KEGG" id="tsr:106545503"/>
    <dbReference type="CTD" id="5733"/>
    <dbReference type="OrthoDB" id="5959154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004957">
        <property type="term" value="F:prostaglandin E receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004960">
        <property type="term" value="F:thromboxane receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014827">
        <property type="term" value="P:intestine smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060455">
        <property type="term" value="P:negative regulation of gastric acid secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000163">
        <property type="entry name" value="Thromboxane A2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001244">
        <property type="entry name" value="Prostglndn_DP_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000265">
        <property type="entry name" value="Prostglndn_EP3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF10">
        <property type="entry name" value="PROSTAGLANDIN E2 RECEPTOR EP3 SUBTYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00428">
        <property type="entry name" value="PROSTAGLNDNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00582">
        <property type="entry name" value="PRSTNOIDEP3R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="58"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013917573.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="39964" checksum="07ABF91C9E7BC878" modified="2020-10-07" version="1">MTFRGLYGGRLNSTPFAMPPFDAAGSRDKGNGSGTFAEQCGSVSVAFPLTMMITGMVGNGLAMLLAYRAYQKKENLRKRPFLLCIGSLALTDLAGQLLTSPVVITVYLANRKWHAVDTSQNLCVFFGLAMTVFGLCPLFIASAMAVERALAIQAPHWYTGHMKTKLTKMVLLCVWLTSFAFALLPMMGIGEYTLQWPGTWCFISIKDHENARVGNAVFASTFAFLGLFSLTVTLACNMATIISLVCRCWSKSSTSRSRKQWGRITMETFIQLLGIMCVLLACWSPLLVTMLKVTFNPNPMNQCRESCNQTQSTEQQPECNFSLAHIRLASLNQILDPWVYLLLRKIFVQKFCQVARAISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5C2</accession>
    <name>A0A6I9Y5C2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Potassium channel subfamily K member 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KCNK18</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913015.1">
        <property type="nucleotide sequence ID" value="XM_014057540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5C2"/>
    <dbReference type="GeneID" id="106541954"/>
    <dbReference type="KEGG" id="tsr:106541954"/>
    <dbReference type="CTD" id="338567"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF346">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0630">Potassium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="98"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="298"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013913015.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="44600" checksum="681A3091C9E14812" modified="2020-10-07" version="1">MKSRTSPSQPKERTCCQVFWAIFPHACFICSLVVYALLGALMFSHVEGCRESHKDEAYRTFMLKLWNLSKDLSDNETNNEIFTNRTHELINQMDFSWFTDPSQNWSFLGSLFFCCTVFTTVGYGHIYPVTRLGKYLCMVYALFGIPLMFLVLTDMGDILATILSISYNRARKLQSEVLARLSFRSCCKKNTDSKGGSASLGAAKIVLLEPLNIKDVLKNNSSFKKKSRQNQNVEIFERLIIRENAFLAPPKLTRSERWNSCPELDKGAMVNHVISNINNLGKKVDKLDVPFSLMIFIVFAYISCAAAILPHWESDLDFEEAFYFCFITLTTIGFGDIKLQHPNFFLFFSVYIIVGIEIVIIAFKLGQDRILGLYKKLLLSVGRKKVSDKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y667</accession>
    <name>A0A6I9Y667_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphofurin acidic cluster sorting protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106547720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PACS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920433.1">
        <property type="nucleotide sequence ID" value="XM_014064958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y667"/>
    <dbReference type="GeneID" id="106547720"/>
    <dbReference type="KEGG" id="tsr:106547720"/>
    <dbReference type="OrthoDB" id="28829at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019381">
        <property type="entry name" value="PACS1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057541">
        <property type="entry name" value="PACS1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280:SF14">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25332">
        <property type="entry name" value="C2_PACS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10254">
        <property type="entry name" value="Pacs-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 N-terminal C2" evidence="5">
        <location>
            <begin position="19"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 C-terminal" evidence="4">
        <location>
            <begin position="463"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="176"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="385"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="707"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="427"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013920433.1"/>
        </source>
    </evidence>
    <sequence length="888" mass="98974" checksum="AB57EC7816EC333F" modified="2020-10-07" version="1">MGTCAAASGVSGGLGPVPVPMNLFATWEIDRSAPSCVPRLCSLRLKKLTVLRELDKELSSVLIAVKIQGSKRVLRSNEYVLPPGGLMETDLELTFSLQYPHFLKRDGNKLQIMLQRRKRYKNRTILGYKTLAVGIINMAEVMQHPTDGGQVLGLHSNIKDVSIRVAEISIYSLSSQPIDHEDGNVPSGPKIKASDRSPDIDNYSEEEDDSFSSEQEASDDAVQGQDLYDEEDEMRKPKKARRKMIRTTSMTRQPNFKQKFVALLKRFKVTDEVLDSDPVDQTQEVEEDLGLLYDSLEDCNNSDSGPEIEDNESVHSTPKPTLRRFFEGVSHSGSQTEISSLHSQKGQERDIIFFLTVVCDFQVLDSDPVDQTQEVEEDLGLLYDSLEDCNNSDSGPEIEDNESVHSTPKPTLSKAENKQLWRPRSMSVKDRQNSKGHSDRTGSLDSEGSPDSRQNSQVPRKSVYDQLNQILVSDEQLPESIILVNITEWQGQYLCDQPQEHKQPMVSTCSMADVQAAFNTIVSRIQRFCNCNSHMPPPVKVAVAGDQSYLSIVLRFFVEQLASKTPDWLNYLRFLVVPLGSHPLAKYLGSVDNRYSTLFLDAAWRELFSRVEAPSTDTVDIPGRVAQFITGANVSHQLPISEAMLTYKQKRKRSLYFDFYISPDEDSCQKFVPFVGVVKVGLVEQSFSTSVDSDDATICSTSLLSSSSPSGGGIPYTKEAIGTPPPSPSVSSGLSGTGNLSPGVEVMGLQVDYWTAQGTEKRKEAEKREIGIKNTLKSNFRSLQVSRIPSPGELTPPNTMAMTVVTKEKNKKVMFLSKKPKEKDLEPKSQVIEGITRLICTAKHQNTMLRVCIDGVEWNDVKFFQLAAQWPTHVKHFPIGIFGYSKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6Z8</accession>
    <name>A0A6I9Y6Z8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Charged multivesicular body protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CHMP3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914425.1">
        <property type="nucleotide sequence ID" value="XM_014058950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6Z8"/>
    <dbReference type="GeneID" id="106543052"/>
    <dbReference type="KEGG" id="tsr:106543052"/>
    <dbReference type="CTD" id="51652"/>
    <dbReference type="OrthoDB" id="2329734at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="204"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="46"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914425.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="27380" checksum="51CD132407B27546" modified="2020-10-07" version="1">MGLFGKTPEKPPKDLRSHFPTFPRVSLSEVGQEGATAQVNEWSLKIRKEMRVIDRQIRDIQREEEKVKRSIKDAAKKGQKDVCIILAKEMIRSRKAVSKLYASRAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQNLVKIPEIQSTMRELSKEMMKAGIIEEMLEDTFESMEDQEEMEEEAELEIDKILFEITAGALGKAPSKVTDALPEQEPEGATAAVVDDEEEDIEAMQSRLATLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y783</accession>
    <name>A0A6I9Y783_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytosol aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LAP3</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914725.1">
        <property type="nucleotide sequence ID" value="XM_014059250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y783"/>
    <dbReference type="GeneID" id="106543273"/>
    <dbReference type="KEGG" id="tsr:106543273"/>
    <dbReference type="CTD" id="51056"/>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013914725.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="14118" checksum="55BC00A946F7B99C" modified="2020-10-07" version="1">MLPRLLLARVRCPLIARPPRALLLLAASMAAGTRAAARQGLILGAFASDHDDQPSQLSDAGEAFDKQVNGKLKELLALSGPPLKKGKTRIFHGLHQVGGKYLTNLTLCLLIFKEIFFLLEEVCGGALRISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7U7</accession>
    <name>A0A6I9Y7U7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RASL10B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013915795.1">
        <property type="nucleotide sequence ID" value="XM_014060320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7U7"/>
    <dbReference type="GeneID" id="106544143"/>
    <dbReference type="KEGG" id="tsr:106544143"/>
    <dbReference type="CTD" id="91608"/>
    <dbReference type="OrthoDB" id="299781at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04142">
        <property type="entry name" value="RRP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000625">
        <property type="entry name" value="Ras-like protein family member 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052661">
        <property type="entry name" value="Ras-like_GTPase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46350:SF2">
        <property type="entry name" value="RAS LIKE FAMILY 10 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46350">
        <property type="entry name" value="RAS LIKE FAMILY 10 MEMBER B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013915795.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="23195" checksum="5D657DB6E790B768" modified="2020-10-07" version="1">MVTTFKIAVLGAQGVGKSAIVRQFLYNEFSEVCVPTKARRVYLPAVVMNGHVHDLQIMDFPPIASFPVNTLQEWADVCCRGLRSVHAYILVYDICCFDSFEYVKTIRQQILETRVIGTSDTPIIIVGNKRDLQKGRVIPRWNVSNLVKKTWKCGYIECSAKYNWHILLLFSELLKSVGCARCKHVHTAIRFQGALRRNRCTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAE6</accession>
    <name>A0A6I9YAE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BET1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BET1L</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Vesicle SNARE required for targeting and fusion of retrograde transport vesicles with the Golgi complex. Required for the integrity of the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920290.1">
        <property type="nucleotide sequence ID" value="XM_014064815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAE6"/>
    <dbReference type="GeneID" id="106547594"/>
    <dbReference type="KEGG" id="tsr:106547594"/>
    <dbReference type="CTD" id="51272"/>
    <dbReference type="OrthoDB" id="261831at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000038">
        <property type="entry name" value="BET1-like protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013920290.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12648" checksum="C086A7E450203AE4" modified="2020-10-07" version="1">MADWGRGPNTNAVDDMLDVENKRRTENLATKVTRLKSLALDIDKDAEDENRYLDNMDSDFMSVTGLLTGSVKRFSTMTRSGRDNRKLLCYVSGGLVLVFFILYYLITRART</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAU7</accession>
    <name>A0A6I9YAU7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">G-protein coupled receptor 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106548229</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921045.1">
        <property type="nucleotide sequence ID" value="XM_014065570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAU7"/>
    <dbReference type="GeneID" id="106548229"/>
    <dbReference type="KEGG" id="tsr:106548229"/>
    <dbReference type="OrthoDB" id="9946711at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF9">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 132-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="35"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013921045.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="38128" checksum="702C03CCC00BD726" modified="2020-10-07" version="1">MNNSSNNECLLVPLEAIKTFKIPIYSIILAFGLPLNLLAVWSLIYQLKKSVVLSVYIMNLVMAHLLQILTLPFWMYYSHNNHHWSLGKTACLFVGVAFRTNFYAKNSFLCLIAMERYLGLVHPLQFHGAQSVRGAVKISILVWLLVLTFCAVGVGLQNRTVDLERKTCLDSSMLNLHYAKFRVSTITFSFIIPFLLMAFFHISVLVKVKQVVSLEKRVKKQISGFISLLIMVFLLLFTPYQVVSYYRYLVEVRAPPATVCDSLRAIFIYEHATLCLSLLDNILDPLFYNLLKNIRTDIRWCLFSRAGRKGLSPEAKISSTFRQCENTRPLPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAW2</accession>
    <name>A0A6I9YAW2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Regulator of G-protein signaling 18-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106548301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921120.1">
        <property type="nucleotide sequence ID" value="XM_014065645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAW2"/>
    <dbReference type="GeneID" id="106548301"/>
    <dbReference type="KEGG" id="tsr:106548301"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08713">
        <property type="entry name" value="RGS_RGS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.196.10:FF:000001">
        <property type="entry name" value="Regulator of G-protein signaling 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034951">
        <property type="entry name" value="RGS_RGS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF242">
        <property type="entry name" value="NOVEL PROTEIN SIMILAR TO VERTEBRATE REGULATOR OF G-PROTEIN SIGNALLING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="202"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013921120.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="37766" checksum="96FBBB95823B2495" modified="2020-10-07" version="1">MLSPLQKCGSEGCLLDCDFLPWQRSTLRKQHQSCEDPEPRNNCEKQEKPCTPSIRLDDTEVVNCPQLPLQDPGGFWRSRSEGRLDQQGVPCPKGTEEGDKTGSGWSLPSPKSLRKERQLCEKVAAAKQHLKNFFGGSNKSLASSVESDIGSDCDGSHHNTQQKQKHPRKKNKKNFLRLWSSGNQDLQSLRPTPEEAHQWAESLEKLLLHPYGRAAFHAFLKSEFSEENLDFWMACEDYRKLRGCDKLQERAKKIFDQYITIQAPKEVNLDSQTRDVTNRNILLPTRSCFDQAQKRIFGLMEKDSYPRFLRSLFYQDLVQPDKEQPNGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBT1</accession>
    <name>A0A6I9YBT1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipid scramblase CLPTM1L</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cisplatin resistance-related protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cleft lip and palate transmembrane protein 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CLPTM1L</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Scramblase that mediates the translocation of glucosaminylphosphatidylinositol (alpha-D-GlcN-(1-6)-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol, GlcN-PI) across the endoplasmic reticulum (ER) membrane, from the cytosolic leaflet to the luminal leaflet of the ER membrane, where it participates in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a lipid glycoconjugate involved in post-translational modification of proteins. Can also translocate 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) (phosphatidylinositol or PI), as well as several other phospholipids (1,2-diacyl-sn-glycero-3-phosphocholine, 1,2-diacyl-sn-glycero-3-phosphoethanolamine), and N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI) in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = 6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71495"/>
            <dbReference type="ChEBI" id="CHEBI:190691"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71491"/>
            <dbReference type="ChEBI" id="CHEBI:57997"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921577.1">
        <property type="nucleotide sequence ID" value="XM_014066102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBT1"/>
    <dbReference type="GeneID" id="106548673"/>
    <dbReference type="KEGG" id="tsr:106548673"/>
    <dbReference type="CTD" id="81037"/>
    <dbReference type="OrthoDB" id="378564at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013921577.1"/>
        </source>
    </evidence>
    <sequence length="484" mass="55537" checksum="517D4F53D84C77EC" modified="2020-10-07" version="1">MLPRRSSLTGLAVGLFAVYVAHTCWVMYGIVHTRPCLAALHEGESNDGCIRPYLARRPKLQLSVYTTTRSNIGTENNIDLVLNVDNFDVETKFERTVNVSVPKKTRNNGTLYAYIFLHHAGILPWHDGKQVHIVSPLTTYMVPKPEEINLLTGEPITQQIETEKKQNYALDEPVSHWRSRLTLNVMVEDFVFDGSSLPADVHRYMKMIQLGKTVHYLPILFIDQLSNRVKDLMIINRSTTELPLTVSYDKISLGKLRFWIHMQDAVYSLQQFGFSEKDADEVKGIFVDTNLYFLALTFFVAAFHLLFDFLAFKNDISFWKKKKSMIGMSTKAVLWRCFSTVIIFLFLLDEQTSLLVLIPAGIGAVIELWKVKKALKMTIEWNGLIPAIKFGAFTESEKKTEEYDTQAMKYLSYLLYPLCIGGAIYSLLNIKYKSWYSWLINSFVNGVYAFGFLFMLPQLFVNYKMKSVAHLPWKAFTYKALSCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC06</accession>
    <name>A0A6I9YC06_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Leucine-rich repeat-containing G-protein coupled receptor 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LGR6</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921594.1">
        <property type="nucleotide sequence ID" value="XM_014066119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC06"/>
    <dbReference type="GeneID" id="106548688"/>
    <dbReference type="KEGG" id="tsr:106548688"/>
    <dbReference type="CTD" id="59352"/>
    <dbReference type="OrthoDB" id="1883493at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15362">
        <property type="entry name" value="7tmA_LGR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000028">
        <property type="entry name" value="leucine-rich repeat-containing G-protein coupled receptor 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF73">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING G PROTEIN-COUPLED RECEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="390"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="484"/>
            <end position="504"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="556"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="602"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="406"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013921594.1"/>
        </source>
    </evidence>
    <sequence length="804" mass="88906" checksum="B979F8205AF0DB05" modified="2020-10-07" version="1">MLQNNHLPRIPAEALWALPNLQSLRLDANLISVVPEKSFEGLPALRHLWLDDNALTEIPVKALNNLPALQAMTLALNRIWRIPDYAFQNLTNLVVLHLHNNQIQHLGIHGFEGLHNLETLDLNYNELVEFPMAIKSLNRLQELGFHNNNIQAIPEKAFAGNPVLQIMTLTQAGIHLLPGGMCQQLASLRVLELAHNHIEKLPSFHQCQKLEEIGLQHNCIHEIGADTFGQLTALRSLDLSWNYIQFIHSEAFLTLHSLIKLDLTDNQLVMLPLAGLGGLTHLKLKGNPALSEAFTKENFPKIRVLEVPYAYQCCPYGACGNVFKISSQWDPEDMSPEDVDGYKRTLGLDHYDLDMDDFQADPDDSKLRTKIQCTPSPGPFKPCDHLFESWVVRLGVWLIMLVSVLCNGLVAVVIFASPGYLSPIKFIIGSIAGANMLMGIYCAILAFVDAVTFGHFAKYGARWEMGAGCQVTGFLSVFASEASILLLTLAAVQCSFSVSCMRTYRKAPSFGTVKAGALSCLLLSSVAAMLPLFSIGEYGASPLCLPHLLPDGKPSMLGFMVALVLVNTLCFLIITGTYIRLYCNLLKGEFDSVWDCAMIKHVAWLIFTNCLLYCPVAFLTFSSMLSLILITPEVIKSVLLVVLPLPACVNPLLYLLFNPHFRDDLCLLKQKSQVQESCAQSCISEDTEKSSCDSTQALVPFSDMDDIFKAPDLFRLPPRRSSVLDAYSFSSVTLIPCQQKAGARGHVSGFSTQASYLADDDLLIASEAREQAKSNLWIALFPKPPSPAFTSHLQEPIHILSVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCC0</accession>
    <name>A0A6I9YCC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Phospholipase D1</fullName>
            <ecNumber evidence="5">3.1.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Choline phosphatase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Phosphatidylcholine-hydrolyzing phospholipase D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC106548779</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="20">Interacts with PIP5K1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Endoplasmic reticulum membrane</location>
            <topology evidence="18">Lipid-anchor</topology>
            <orientation evidence="18">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013921704.1">
        <property type="nucleotide sequence ID" value="XM_014066229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCC0"/>
    <dbReference type="GeneID" id="106548779"/>
    <dbReference type="KEGG" id="tsr:106548779"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032534">
        <property type="term" value="P:regulation of microvillus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000114">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000021">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000005">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF57">
        <property type="entry name" value="PHOSPHOLIPASE D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="24">
        <location>
            <begin position="79"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="23">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="26">
        <location>
            <position position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00065403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00074658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013921704.1"/>
        </source>
    </evidence>
    <sequence length="576" mass="66734" checksum="38FB6F169E563DA0" modified="2020-10-07" version="1">MSLTNRRQVNTSSLKKIAADMSNLIESLDSRELHFEGEELDSDVVHDPKAPVGIPFSAIYNTKGFKEPQVQLYLSGCPIRVRVLEVERFTSTTKVPSPRVYTIELTHGEFTWQVKRKYKHFQELHRELLRYKAFIRLPIPTRRHTVRRQTVKREEARQIPSLPRTSENMVRDDHLSSRRKQLEDYVANLLKMPLYKNYHGTMEFLGVSQLSFIHDLGPKGIEGMIMKRSGGHRIPGLNCCGQGRLCYRWSKRWLVVKDSFLLYMKPDSGAVSFVLLVDKEFSIKIGKKETETKYGLRIDNLSRSLILKCNSYRHALWWGQGIEEFVQKNGKNFLKHHRFGSYAAIQENTLAKWYVNAKGYFEDIANAMEAAKEEIFITDWWLSPEIFMKRPVVEGNRWRLDCILRRKAQQGVRIFVILYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRITANKSESSVNLSSLAETVQPQVETPVTTPLQGCSDDAQEISRMKGVGRTKKFVRFSIYKQLHRHTMHRADSVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCI5</accession>
    <name>A0A6I9YCI5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Polycomb protein SUZ12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SUZ12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921871.1">
        <property type="nucleotide sequence ID" value="XM_014066396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCI5"/>
    <dbReference type="GeneID" id="106548898"/>
    <dbReference type="KEGG" id="tsr:106548898"/>
    <dbReference type="CTD" id="23512"/>
    <dbReference type="OrthoDB" id="166746at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21740">
        <property type="entry name" value="C2_II_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21551">
        <property type="entry name" value="VEFS-box_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21750">
        <property type="entry name" value="ZnB-Zn_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019135">
        <property type="entry name" value="Polycomb_protein_VEFS-Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057540">
        <property type="entry name" value="Znf_SUZ12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF0">
        <property type="entry name" value="POLYCOMB PROTEIN SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09733">
        <property type="entry name" value="VEFS-Box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23320">
        <property type="entry name" value="Zn_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Polycomb protein SUZ12-like zinc finger" evidence="10">
        <location>
            <begin position="465"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Polycomb protein VEFS-Box" evidence="9">
        <location>
            <begin position="591"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="401"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="728"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="732"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013921871.1"/>
        </source>
    </evidence>
    <sequence length="778" mass="86644" checksum="7463224EA8DE82AE" modified="2020-10-07" version="1">MAGRDLRRPIKRDGAIAGARGGGGGGNAGPSSGAPSGGGGATATGGGAGGSFGGFTSAAAPGGKSGGAVGGGGGGGGGGGGSYSGGGGGSSASSAAAGSAAALPPVKKPKMEQIQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFRVDGMLTKVEKTKGEHDLQSLSAHLHLTFTGFFHKNDKPLQNSENEQSSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDTNQIKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNSSNREDEEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKPATPIAKPLSTRNSESLPQENKSSSVKPTQTIAVKESLPSDLQTRKERDVTNEPRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGHKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQHKLEKGESITATSSEEFTEEQTGATNGYSDANSKERSSEIDNTSCMTKQSRKQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCJ6</accession>
    <name>A0A6I9YCJ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">WD repeat and FYVE domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">WDFY3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921886.1">
        <property type="nucleotide sequence ID" value="XM_014066411.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106548914"/>
    <dbReference type="KEGG" id="tsr:106548914"/>
    <dbReference type="CTD" id="23001"/>
    <dbReference type="OrthoDB" id="10018316at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15719">
        <property type="entry name" value="FYVE_WDFY3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000028">
        <property type="entry name" value="Putative hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056252">
        <property type="entry name" value="Alfy-like_Arm-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051944">
        <property type="entry name" value="BEACH_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108">
        <property type="entry name" value="BLUE CHEESE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108:SF1">
        <property type="entry name" value="WD REPEAT AND FYVE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23295">
        <property type="entry name" value="Arm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0853">WD repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BEACH" evidence="13">
        <location>
            <begin position="1650"/>
            <end position="1960"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="2107"/>
            <end position="2148"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="2400"/>
            <end position="2460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2219"/>
            <end position="2272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="2221"/>
            <end position="2234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921886.1"/>
        </source>
    </evidence>
    <sequence length="2471" mass="276808" checksum="41A0FEC0040EC356" modified="2020-10-07" version="1">MDSHNVRNIQAFSVLQNAFLKAKTSYLAQIILDAILNIYIADNANYFILESQHTLSQFAEKISKLPEVQTKYFEMLEFVVFSLNYIPCKELISVSILLKSSTSYSCSIIATRTLLKFTRHDYIFKDVFREVGLLEVMVNLLHKYAALLKDPTQALNDQGLLKLCRLQLPISVDGVFEFRCWYYCNYSFSLVHIVGIFREFGGARCVHNIVKYPQCRQQALMIIQQLVLSPSGDDDMGTLLGLMHSAPPTELQLKTDILRALLSVLKESHRTRTVFRKVGGFVYITSLLVAMERSLCSPLKNGWEKVNQNQVFELLHTVLCTLTAAMRYEPANSHFFRTEIQYEKLADAVRLLGCFSDSRKLSPVNVFPSNTQPFQKLLEDDLVSMDTVSPTLKHCSKLFIYLYKVTTDSFDSRAEQIPPCLTNESSLPSPWGTPALSRKRHAYHSVSAASSVFPSKNATDAKLHKGTILLQNLDAVIIHPGAMLAMLDLVVSVGSTTQPEHALDLQLAVANILQSLVHTERNQQVMCEGGLHARLLQKCSAALTDEDHSLHPPLQRMFERLASQALEPMVLREFLRLGNPLNCGAWDKKQLKRYRVHKPSSLSYEPEMRNSMVTSMEGLGTDCVFSLHEDSHYRISKSLLKPAEGSTVPLTRVKCLVSMTTPHDIRLHGSSVTPAFIEFDTSLEGFGCLFLPSLAPHNAPTNNVVTTGLIDGTVVSGIGTGERLFPPPSGLSFSSWFCVEHFSTAPNYHPIRLLTVVRRANSSEQHYICLAIVLSVKDRSLIVSTKEELLQNYADDFNEESSCYELLPCCARFRCGDHIVEGQWHHMVLVMSKGMLKNSTAALYIDGQLINTVKFHYIHSSPGGSSCANPPIVSTVYAYIGTPPAQRQLSSLVWRLGPTHFLEEVLPPSSISTIYELGPNYVGSFQAVYMPCKDSKAEGISPSPVSLVPEEKVSFGLYALSVSSLTVARIRKVYNKLDSKAIAKQLAISSHENATPVKLLHNSGGHLNGPARTIGAALIGYLGVRTFVPKPVATTLQYIGGAAAILGLVAMASDVEGLYAAVKALVCVVKSNPLASKEMERIRGYQLLAMLLKKKRCLLNSHILHLTFSLVGTVDSGHETSIIPNSTAFQDLLCDFEVWLHAPYELHLSLFEHFIELLTESSEASKNAKLMREFQLIPKLLLTLRDVSLSQPTIAAISNVLSYLLQGFPSSNDLLRFGQFIASTLPTFAVCEKFVVMEMNNEEKLDTGTEEDFGGLVSANLILLRNRLLDILLKLMYTSKEKTSVNLQACEELVRTLGFDWVVLFMEDHLHPATVTAAMRILVVLLSNPTILIKFKEGLSGGGWLEQTDSVLTNKIGTVLGMSPHKSPCYQNTLQVSGLEAIFCIGPSGLPRQQNALKYVTEEWSQIEYELLRERGLWGPPIGSHLDKWMLEMTEGPCRMRKKMVRNDMFYVQYPYMPDTEHETNLLKQVTDLDDDAGSPAEEFKAFASDTGMNRSQSEYCNVGSKTFLTNHPAKKFVFDFMRVLIIDNLCLTPASKQTPLIDLLLEASPERSTRAQQKEFQTYILDSVMDHLLAADVLLGEDASLPITSGGSYQVLVNNVFYFTQRVVDKLWQGMFNKESKLLIDFTLQLIAQSKRRSQGLSLDAIYHCLNRTILYQFSRPHKTVPQQVALLDSLRMLTVNRSLILGPANHDQEFISCLAHCLINLYAGRKPWQSEEEGSWLREYPVTLMQFFRYLYHNVPDLATMWMSPDFLCALAATVFPFNIRPYSEMEISQWMFTHIAVVKDLVDMQYKEYQEGGHFDLADRMFHSVREAWYSASKHNMADVKELIPEFFYLPELLLNSNNFDLGCKQNGTKLGDVILPPWAKGDPREFIRVHREALECDFVSAHLHEWIDLIFGYKQQGPAAVEAVNVFHHLFYEGQVDIYNINDPLKETATIGFINNFGQIPKQLFKKPHPPKRVRSRINGEMSGISVPPGITNDKIFSHHLDNLKPSLAPVKELKELVGQIVCTDKGILAVEMNKVLLPPTWNKTFAWGYADLSCRLGAYESDKAVIVYECLSEWGQVLCAICPNPKLVITGGTSTVVCVWEMGTSKEKAKMLTLKQALLGHTETVTCLTASLAYHIIVSGSCDRTCIIWDLNQLSFVTQLRGHRAPVSALCINELTHRLAGAGANNGSSPHYARLSSNLPGDKLSFLITEPSRRPSVVIKAFIFAFQAEGQEGPEEESSDSDAEDQAPAHDAKLPESQPQQAPAGHRPRSSSARATAVWSVETSSEDTRRWSDHLSLDEKDGFIFVNYSDGQAKGYSHAPTNHSNANQGELRHYSKLKPGYRWERQLVFRSKLTMHTAFDRKDNAHPAEITALAISKDHSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEGGDSCSSCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKRLKISSPVRVCQNCYYNLQHERGPEEGSWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD58</accession>
    <name>A0A6I9YD58_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106549064</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922088.1">
        <property type="nucleotide sequence ID" value="XM_014066613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD58"/>
    <dbReference type="GeneID" id="106549064"/>
    <dbReference type="KEGG" id="tsr:106549064"/>
    <dbReference type="OrthoDB" id="435430at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08834">
        <property type="entry name" value="ArfGap_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13251">
        <property type="entry name" value="PH_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000001">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000006">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000036">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF5">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="302"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="12">
        <location>
            <begin position="417"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="615"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="758"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="773"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="821"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="854"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013922088.1"/>
        </source>
    </evidence>
    <sequence length="877" mass="99481" checksum="210DF194D85121FA" modified="2020-10-07" version="1">MTDLISVQDFISQTQEDLSSPTTSSFTSHMGKCRNTVCSLEEDLDSDRNVLQKMRKAVKAEHTTGQDLISHLEAHIAALEKFSGNYHNSGDEKLSEAFTTFASFSQELLEAIKSLLLSLYHNIRFSLDSLIKGDFKGDFKKPVEKAWKDYESKLAKIEKEKRELAKQYGMVRTEVAGGEIAEEMEKERRMFQLHMCEYLIKVNELKTKKGVDLLQNNIKHCNSQFNFFQKCLKATEKHKQFIEKLTPEIQSMKSKQEEEKKQLYSLRDQLKSALQLEPREQNSVSKPVRYSMHQLQGNKQYGTERSGNLFKKSDGLRKVWQKRKCTVRNGYLTISHSTLNRLPVKLNLLTCQVKPNADDKKCFDLIAHNRTYHFLAEDEQECNIWISVLSNSKEEALNVAFKQAHNKDEYNTENLTGAIIEEIKHMPGNNICCDCSTPDPAWLSINLGILICIECSGIHRELGVHHSRIQSLALDKLATSELLLAKNIGNLRFNDIVEANLPNSSSKPTKESAMAHRKLFIISKYVDRKYVVPRIMRSPVAKLKNLENAVKEKDIFILLQAYAEKMELSEPVLEFSQEPRETLLHLAVLGSDRTSLHIVDFLVQNSGSLLKQTSKGNTPLHYCSLHNKPECLKLLLRAKANIKITNEAGETALDIAKLLNHTTCEDLLLQAENNQFNMRAHVEYEWQPGQEEMFESDDELDEKLCLVKKDRFHRPQSCYNVLGTASQSWKNPADFFRGTNPASQDKCHDLYATPHASRSAEIASPPTYACSGPPLPPRNAPRAPSFPPPLPPTSKPAYLVNTATERAVPPPLTIRHKRTSSEPVPSTPLNHSPSDDSRSFSLPRRCSPEGASPRSFSSQPSELSEDSWQQANYDQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD64</accession>
    <name>A0A6I9YD64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106549012</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Arkadia family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013921999.1">
        <property type="nucleotide sequence ID" value="XM_014066524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD64"/>
    <dbReference type="GeneID" id="106549012"/>
    <dbReference type="KEGG" id="tsr:106549012"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
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    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="129"/>
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    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921999.1"/>
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    <sequence length="140" mass="16330" checksum="CBF969C71068A196" modified="2020-10-07" version="1">MNISYDENEGQMTDDEDEEECDKSYEEALDQTHVVHEIRNYPQFQLLALQGLNPNRHTSAVRESHERWPQEDKIEKEEGEESDTDEKCTICLSMLEDGEDVRRLPCMHLFHQVCVDQWLANSKKCPICRVDIETQLGSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF04</accession>
    <name>A0A6I9YF04_9SAUR</name>
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        <recommendedName>
            <fullName evidence="5 6">Prefoldin subunit 4</fullName>
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        <name evidence="9" type="primary">PFDN4</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <text evidence="3 6">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
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        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Interacts with URI1; the interaction is phosphorylation-dependent and occurs in a growth-dependent manner.</text>
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        <text evidence="1 6">Belongs to the prefoldin subunit beta family.</text>
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        <source>
            <dbReference type="RefSeq" id="XP_013922747.1"/>
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</entry>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
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        <location>
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        <location>
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            <end position="420"/>
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    </feature>
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        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013922867.1"/>
        </source>
    </evidence>
    <sequence length="512" mass="56571" checksum="FF3B0BF286059B37" modified="2020-10-07" version="1">MTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGNAPLDHLPLNRITTMRKRYRNGLGPVKEGEAQYAVVHCTGYIKAWPPAGMTIPEEDAEVGQGSKYCLVAIGRLQVTSSPVCMDMNGMSVPTEFLSRHSSDGIITFVDPRCISVIGYQPQDLLGKDILEFCHPEDQSHLRESFQQVVKLKGQVLSVMYRFRTKNREWMLIRTSSFTFQNPYSDEIEYIICTNTNVKQLQQQQAELEVHQRDGLSSYDLTQVPVPSIQSNVHEGGKAVEKPDTIFTQERDPRFAEMFAGITASDKKMMPSASTTGNQQIYSQGSPFQPGHSGKSFSSPVVHVPGVNDIQSSSSTGQNLSQISRQLNQSQVAWTGSRPPFSGQQIPSQASKAQSSPFAIGSSHSYPTDPASFSPLSSPATSSPSGNAYSSLANRTTGFAESSQSSGQFQGRPSEVWSQWQSQHHNQQNSEQHPHQQTSQTEVFQDMLPIPGDPTQGTGNYNIEDFADLGMFPPFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFW4</accession>
    <name>A0A6I9YFW4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vesicle-associated membrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">VAMP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923067.1">
        <property type="nucleotide sequence ID" value="XM_014067592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFW4"/>
    <dbReference type="GeneID" id="106549841"/>
    <dbReference type="KEGG" id="tsr:106549841"/>
    <dbReference type="CTD" id="6843"/>
    <dbReference type="OrthoDB" id="10042941at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000013">
        <property type="entry name" value="Vesicle-associated membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013923067.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="13943" checksum="E9667780022398AF" modified="2020-10-07" version="1">MILSGGNARSSARVDGRPVGTGQEGGENAGGPGAPPNMTSNWRLQQTQAQVEEVVDIMRVNVDKVLERDQKLSELDERADALQAGAQVFESSAAALKRKYWWKNCKMMIMLGVICAIVVIAIALYFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG12</accession>
    <name>A0A6I9YG12_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Activator of basal transcription 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ABT1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923208.1">
        <property type="nucleotide sequence ID" value="XM_014067733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG12"/>
    <dbReference type="GeneID" id="106549972"/>
    <dbReference type="KEGG" id="tsr:106549972"/>
    <dbReference type="CTD" id="29777"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013923208.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="30393" checksum="66041A377DE4FBA2" modified="2020-10-07" version="1">MVDDGAGKLPLTPRIEDSVMDSGEEEVETVSEDKETEEDSQLDSCNQNDAKKVVPGIVYLGHIPPRFRPRHVRNLLCVHGEVGRIFLQPEERFIRKKKKAAGSNAKNFTEGWVEFRDKRVAKLVAASLHNTPIGVRKKSKFHYDLWNIKYLNRFKWTHLSEQLAYERQVRQQRMRAEVSQAKRETNFYLQNVEKSKHFLKKDSQKGHAEKSWGFVQRCTEDEIQTSKGKKRLKQQLARSAEIQQKSQSNKSLLTKIFNIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGI5</accession>
    <name>A0A6I9YGI5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein arginine N-methyltransferase 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923020.1">
        <property type="nucleotide sequence ID" value="XM_014067545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGI5"/>
    <dbReference type="GeneID" id="106549801"/>
    <dbReference type="KEGG" id="tsr:106549801"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000664">
        <property type="entry name" value="Protein arginine methyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF47">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923020.1"/>
        </source>
    </evidence>
    <sequence length="163" mass="18448" checksum="0A2031D9648396D2" modified="2020-10-07" version="1">MLGGVGHLTIGRAVPSDDSECPQMEKTHSSAHLGTYRVSAQNEKPLKVKSVVADQFPYGKHEGITIECSSISDYSEKIIKSNHLDHIITIFKGKVEEIDLPVDKVDIIISEWMGYSLFYESMLNTVIFARDKWLKPGGLMFPDRAALYMVAIEDRQYKDFKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI44</accession>
    <name>A0A6I9YI44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EEF2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924028.1">
        <property type="nucleotide sequence ID" value="XM_014068553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI44"/>
    <dbReference type="GeneID" id="106550617"/>
    <dbReference type="KEGG" id="tsr:106550617"/>
    <dbReference type="CTD" id="1938"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000058">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF35">
        <property type="entry name" value="ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="17"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013924028.1"/>
        </source>
    </evidence>
    <sequence length="155" mass="16711" checksum="5AC200125A2EFF74" modified="2020-10-07" version="1">MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASSRAGETRFTDTRKDEQERCITIKSTAISLFYELSEHDLAFIKQSKDGCGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGKKLASHFPLGTLSFAGGCGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI59</accession>
    <name>A0A6I9YI59_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium/potassium/calcium exchanger 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106550192</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923525.1">
        <property type="nucleotide sequence ID" value="XM_014068050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI59"/>
    <dbReference type="GeneID" id="106550192"/>
    <dbReference type="KEGG" id="tsr:106550192"/>
    <dbReference type="OrthoDB" id="2127281at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000006">
        <property type="entry name" value="sodium/potassium/calcium exchanger 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF21">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="64"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923525.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="39195" checksum="A656E4921FB093CB" modified="2020-10-07" version="1">MAKLGQESAVVNPSVLSELWRNRKLMASHNESHKKNCTEPAIHEFPQDFFKNKERQQGAILLHILGALYMFYALALVCDDFFVPSLEKICEKLHLSEDVAGATFMAAGSSTPELFASVIGVFITHGDVGVGTIVGSAVFNILCIIGVCGIFAGQVVRLTWWSVCRDSAYYTLSVIVLIVFIYDEKIVWWESLILIIMYFFYILIMKFNMKMQKFFTIKSMNVGNGNTVNSELEDVKGTQEPGRNSVVMVDEIICSSPPKYRFPEAGLRIMITNKFGPRTRLRMASRLIINERQRLIHSSNGQLIQNGRHENIENGAIHAENQEEAGHIAQSPFSPPGLGILGVEVYKPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIA1</accession>
    <name>A0A6I9YIA1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ataxin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ATXN1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATXN1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923560.1">
        <property type="nucleotide sequence ID" value="XM_014068085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIA1"/>
    <dbReference type="GeneID" id="106550220"/>
    <dbReference type="KEGG" id="tsr:106550220"/>
    <dbReference type="CTD" id="6310"/>
    <dbReference type="OrthoDB" id="10000452at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020997">
        <property type="entry name" value="Ataxin-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043404">
        <property type="entry name" value="ATAXIN1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003652">
        <property type="entry name" value="Ataxin_AXH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036096">
        <property type="entry name" value="Ataxin_AXH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13392">
        <property type="entry name" value="ATAXIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13392:SF5">
        <property type="entry name" value="ATAXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12547">
        <property type="entry name" value="ATXN-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08517">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00536">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102031">
        <property type="entry name" value="AXH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51148">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AXH" evidence="10">
        <location>
            <begin position="549"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="42"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="744"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="745"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923560.1"/>
        </source>
    </evidence>
    <sequence length="802" mass="85654" checksum="13C3C09CBDBD018A" modified="2020-10-07" version="1">MKSNQERSNECLPPKKREIPATSLPSEVKPLFLASENHRADNITWLSSTTSSHSSGRNRSGGTSVEAGSQQGVGLHKSLSTGMDYSPPSAPRSVPASATLSTVYPSALSQAGTPVSPVQYTHLQHAFQFVGPQYSGPYAGFIPSPLISPTANSATTTAASATAVATTPSQRSQLEAYTSLLATMSNLSQQSQKVEPHLVRTPGLITAGSPPPIQQNQYVHISSSQSAVRNVSPPAIPVHLHPHQTVIPHAFALGPSSQVVVQYADSGGHFVTREPTKKSESGRLQAMQAKEILNGEVDKNRRYGISPSSEVNLLKSGNKPPPHHYEARHVVVHSGPTDYIARDSSAVRPSVMVVPNSSTPTADMEVQQSANRETPPSAINEKGSLHLGKTGHRSYALSPQQTIGHEGVKAVATLSPHTVIQTTHSASEQLPVGLPATAFYTGTQPPVISYLSSQQQAFGYPGSLPQHLVIPGTQPLLIPVGSADIDSSGMPPAIATSSPQLAAVPHTFVTTAIPKSENFNAESLVNQTAYQAAVVQAQIHLPLVQPVPPSATAPAILPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISSDLKIDSSTVERIDDSHNPGIAMIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQSMDSASILLKHSKNDSLVGSRHRYVEQENGIDQGGVKMTSENGELKFPNKIGLPAASLLTKSESSKPTATRKRRWSAPETRKLEKSEEEPPLTLPKPSFIPQEVKICIEGRSNVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIZ2</accession>
    <name>A0A6I9YIZ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AP-3 complex subunit mu-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AP3M2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924323.1">
        <property type="nucleotide sequence ID" value="XM_014068848.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550865"/>
    <dbReference type="KEGG" id="tsr:106550865"/>
    <dbReference type="CTD" id="10947"/>
    <dbReference type="OrthoDB" id="870at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="6">
        <location>
            <begin position="88"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013924323.1"/>
        </source>
    </evidence>
    <sequence length="182" mass="20418" checksum="54B733B0E638D378" modified="2020-10-07" version="1">MSLDYFGVCSEVVIKDNVVVVYEVLEEMLDNGFPLATESNILKELIRPPTILRTVVNTITGSTNVGEQLPTGQLSVVPWRRTSVKYTNNEAYFDVIXFIFLSAVINSNGSTITAEIQGVIDACVKLTGMPDLTLSFMNPRLLDDVSFHPCVRFKRWESERILSFIPPDGNFRLLSYHVSAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ23</accession>
    <name>A0A6I9YJ23_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">LOW QUALITY PROTEIN: pappalysin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106550796</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M43B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924242.1">
        <property type="nucleotide sequence ID" value="XM_014068767.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550796"/>
    <dbReference type="KEGG" id="tsr:106550796"/>
    <dbReference type="OrthoDB" id="536211at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04275">
        <property type="entry name" value="ZnMc_pappalysin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000097">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000026">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043543">
        <property type="entry name" value="PAPPA/PAPPA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008754">
        <property type="entry name" value="Peptidase_M43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46130">
        <property type="entry name" value="LAMGL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46130:SF2">
        <property type="entry name" value="PAPPALYSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00066">
        <property type="entry name" value="Notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05572">
        <property type="entry name" value="Peptidase_M43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00004">
        <property type="entry name" value="NL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LamG-like jellyroll fold" evidence="8">
        <location>
            <begin position="106"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="LNR" evidence="7">
        <location>
            <begin position="391"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="LNR" evidence="7">
        <location>
            <begin position="434"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924242.1"/>
        </source>
    </evidence>
    <sequence length="716" mass="81328" checksum="B54D16EB9B0EB4AF" modified="2020-10-07" version="1">MANLLGTECQKSYQGCTLEKQDFRVLAAMRVQPSAGWLARGRRSSSTSAAGALEAPRVPRRRRRAPDEGPGTNEGRRLLAPPSRAFYFRGQGDQLRLKAELEIPRDAFTVQVWLRPEGGQKAPAVIAGLYDKCSYTTLDRGWVLGINNVSDQGNRDPRYFFSLKTDRARRVTTITDHHSYLPNQWVHLAITYDGRLMKMYVNGAQVASSREQVGSIFSPLTLKCKILMLGGNTLNQNYRGYIEHFSLWRTVRSQKEILLDMTLVAHEVDVPLPQLVFQETLVNVKSRWSPMKDSPRLPLTEFTFHSGYLLDTSLAPPLCGQTVCDNMEVIASYNQIPTFRHRKVVRYRVVNIYDDRHRNPTISQQQIEFQHRQLNDAFSPYNISWEIEVLEINDSFLRDRLILANCEISKIGDESCDLECNHTLTGFDGGDCRHVRQLSFHKKKQNGVCDMDCNSEKYNFDGGDCCNPDITDVTKTCFDPDSPNRAYLDVKELKNRLNLNGSTHLNIFFANSSEEELAGMATFPWDKEALVHLGGIVLNPAFYGVAGHTHTMIHEVGHSLGLYHVFKGVSEIFSCSDPCMETEPSFETGDLCHDTNPTPTHKTCADPPANSNMCGFRNFQNTPFNNFMSYADDDCTNSFTPNQVARMHCYLDLVYQSWQHIKKPAPIAITPQIVDRTEASVTLEWFPPIDQHFFERXMGGTTCEYQLRKLSMIQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ70</accession>
    <name>A0A6I9YJ70_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Arachidonate 5-lipoxygenase-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106550940</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homotrimer. Interacts with LTC4S and ALOX5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924403.1">
        <property type="nucleotide sequence ID" value="XM_014068928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ70"/>
    <dbReference type="GeneID" id="106550940"/>
    <dbReference type="KEGG" id="tsr:106550940"/>
    <dbReference type="OrthoDB" id="8659873at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004464">
        <property type="term" value="F:leukotriene-C4 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019370">
        <property type="term" value="P:leukotriene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001446">
        <property type="entry name" value="5_LipOase_AP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050997">
        <property type="entry name" value="MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250:SF2">
        <property type="entry name" value="ARACHIDONATE 5-LIPOXYGENASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00488">
        <property type="entry name" value="5LPOXGNASEAP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924403.1"/>
        </source>
    </evidence>
    <sequence length="80" mass="8843" checksum="FAC81DCC02F16007" modified="2020-10-07" version="1">MDQDMVGYIVLLVIVTLLSVIQNAFFASKVEQESKSSTGKTNQKCCPSAFERVFTANQNCRDAYPTFLAVLWCAGLLCSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKJ0</accession>
    <name>A0A6I9YKJ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BCL-6 corepressor-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106551222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924752.1">
        <property type="nucleotide sequence ID" value="XM_014069277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKJ0"/>
    <dbReference type="GeneID" id="106551222"/>
    <dbReference type="KEGG" id="tsr:106551222"/>
    <dbReference type="OrthoDB" id="3666223at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047144">
        <property type="entry name" value="BCOR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24117">
        <property type="entry name" value="AGAP007537-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24117:SF6">
        <property type="entry name" value="BCL-6 COREPRESSOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="457"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="616"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="693"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="775"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="267"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="659"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="702"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="815"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924752.1"/>
        </source>
    </evidence>
    <sequence length="938" mass="99157" checksum="CD11665418FA4991" modified="2020-10-07" version="1">MISAAPLYSGVHNWTGTERLHTCSLEESLPFSQVEITAVSGSGGGAEGFEPEGTVEKEAGPQLREQAHEPDCAQKALKDSDAKAGQLQEPEEAPQPDKSSRRSSLDCPFASAAETAEVTPICPKAPESEQMGKEKGLPAPAASVNPITATSSSSCSTGAPAAATYTLKSVCFSTSPAPPMQKMSLSLPPGATVLAPSQPLVYLPPLSNALGVSPALALPVLSSFPQDPCLPGLHPYPYTFSVGRPLAPDPKAACVEAAQLSCPPKPSGENSSTPTTPGSGPPANGPELVSSTPALAASCASVVPLSGPATVLPRVPERLATGVLASISPLKSPPQLEREMVSSPECSEMPLDLSSKSNRQKLPPPSQRKTPPMPVLTPVHPSGKALLTPVFSKAQRVAQAAASPFVIFPELLRNGEQSTWVKNPTAFIGTIPGTYMGVANPVPASVLLNKDVGADLSSRDSRHLPKQEPICIIDQGEPRSAGLPSGKKNGSEGQADPTRRTPAPPLRPSKELSLWNPSQANLYSRCSLNGKPASPQLLPIGWSHYHPGTLLSIGISATAQLPPNPVGPCKTPGPSEPAVFPAGQLAEATPVVQKPPEAPSQGKGCKAALPSTESPLCVSPALQTNPPEAKGSRGLERRETDSAGESTLLPPPETPPEGCAHKHSADPKAKSPFLPLHPAHALPLASHASIRVTAESASPAEKQCRERRGDTSRKAPSVAVPPCPVHQVDLSRIKKERVEPEPPFASATCLHTGATLRDLPKAKVKGVAHIKVEGGFPCKSKRHHEGPDRPAHKKLKSRGGEELGSPCKASSQNQHSRKWRKHHSNLHETTDDGRLGCVKERGNLRAKRKRRKLSPGHRGHSCEEGKGRSGWVVPIPSLIQGCPTFPGATWKIWSFFFSRLPQGDLFGLPELFLVIFLLQIHFCEVIKHYKIQKQLRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLQ4</accession>
    <name>A0A6I9YLQ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Neurturin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106551382</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Growth factor that supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Involved in the development of the neural crest. Might control the size of non-neuronal cell population such as haemopoietic cells. Acts by binding to its coreceptor, GFRA2, leading to autophosphorylation and activation of the RET receptor. Heparan sulfate-binding is required for signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer; disulfide-linked. Interacts with GFRA2 coreceptor and RET: forms a 2:2:2 ternary complex composed of NRTN ligand, GFRA2 and RET receptor. Also forms a 4:4:4 tetrameric complex composed of 4 copies of NRTN ligand, GFRA2 and RET receptor, which prevents endocytosis of RET.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TGF-beta family. GDNF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924959.1">
        <property type="nucleotide sequence ID" value="XM_014069484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLQ4"/>
    <dbReference type="GeneID" id="106551382"/>
    <dbReference type="KEGG" id="tsr:106551382"/>
    <dbReference type="OrthoDB" id="9936891at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030116">
        <property type="term" value="F:glial cell-derived neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035860">
        <property type="term" value="P:glial cell-derived neurotrophic factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19383">
        <property type="entry name" value="TGF_beta_Neurturin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000037">
        <property type="entry name" value="neurturin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043401">
        <property type="entry name" value="GDNF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173">
        <property type="entry name" value="GDNF SUBFAMILY OF TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173:SF3">
        <property type="entry name" value="NEURTURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="11">
        <location>
            <begin position="54"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013924959.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="18157" checksum="37A970BD83F7D3D0" modified="2020-10-07" version="1">MCFRFRIGSEDSTLFESYTEGEIHQLISTLIERYSQSMNSGGHELPLFAKAGNRMKRAKARHKPCSLKELEVTISELGLGYNSDETVLFRYCSGTCDSAVRNYDVSLKHVRSMRKIRKEKVRARPCCRPLSYDDDVSFLDTGNRYYTVNEVSAKECGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLY9</accession>
    <name>A0A6I9YLY9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PDZ domain-containing protein GIPC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GIPC2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GIPC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925009.1">
        <property type="nucleotide sequence ID" value="XM_014069534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLY9"/>
    <dbReference type="GeneID" id="106551425"/>
    <dbReference type="KEGG" id="tsr:106551425"/>
    <dbReference type="CTD" id="54810"/>
    <dbReference type="OrthoDB" id="6509831at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd21180">
        <property type="entry name" value="GH2_GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23078">
        <property type="entry name" value="PDZ_GIPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000097">
        <property type="entry name" value="PDZ domain-containing protein GIPC1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055349">
        <property type="entry name" value="GH2_GIPC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056814">
        <property type="entry name" value="GIPC1-3_GH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017379">
        <property type="entry name" value="GIPC1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12259:SF3">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN GIPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12259">
        <property type="entry name" value="RGS-GAIP INTERACTING PROTEIN GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25083">
        <property type="entry name" value="GIPC1_GH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25082">
        <property type="entry name" value="GIPC1_GH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038083">
        <property type="entry name" value="UCP038083_GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="130"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925009.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="35561" checksum="6B98B636F92065F7" modified="2020-10-07" version="1">MPLGLRGKKKGKSRETSTLVDGEAPPARGRGDDEVGVGARSGAVGAAASRGPVRLVFHTQLAHGSPTGRVEGFSSVKELYAKIAEAFRIAPAEIMFCTLNTHKIDMNKLLGVQIGLEDFIFAHVKGIKKEVEVFKSEDSLGLTITDNGNGCAFIKRIKAGSLIDQIKVICVGDHIESINGKNVAAYRHYDVAKNLKDLKKGQTFTLKLIEPMKSFEIIEPRSKSGNFAEEKICRGRETLRLRAKGSATVEEMLSDVEEKATKKVDDLLEMYMGIRDLELAATMVEAGKGKNNPDEFAIALDEALGDFAFPDEFVFDVWGAIGDTLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMK5</accession>
    <name>A0A6I9YMK5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">DnaJ homolog subfamily A member 3, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">DnaJ protein Tid-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Tumorous imaginal discs protein Tid56 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">DNAJA3</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with JAK2, HSPA9B and IFN-gammaR2 chain. Interacts with Ras GTPase-activating protein 1 (RASA1). Isoform 2 interacts with MUSK (via the cytoplasmic domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Postsynaptic cell membrane</location>
            <topology evidence="19">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925628.1">
        <property type="nucleotide sequence ID" value="XM_014070153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMK5"/>
    <dbReference type="GeneID" id="106551977"/>
    <dbReference type="KEGG" id="tsr:106551977"/>
    <dbReference type="CTD" id="9093"/>
    <dbReference type="OrthoDB" id="10256793at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000003">
        <property type="entry name" value="dnaJ homolog subfamily A member 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000025">
        <property type="entry name" value="dnaJ homolog subfamily A member 3, mitochondrial isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11 25" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 25" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="27">
        <location>
            <begin position="86"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="28">
        <location>
            <begin position="218"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="25">
        <location>
            <begin position="218"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="437"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00063025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00073672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00076397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00081755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013925628.1"/>
        </source>
    </evidence>
    <sequence length="475" mass="51536" checksum="FA6552B92EC5AC0F" modified="2020-10-07" version="1">MAAGCVSRWLAEAATAAGSCRAGGGEPRRLALVLALARWRSVAPVASRAALAPRVGNHCGTRPLPLVWAASFHTSRSFSSSSSKEDYYQILGVPRSASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLGDEVKRKQYDAYGTADFGFGSGSAGSGQQYRHTGPTIDPEELFRKIFGEFSQSPFGDFSTVFDQPQEYIMDLTFSQAAKGVNKQITVNINDNCQRCDGKGHEPGTRVVHCSYCNGTGTETINTGPFVMRSTCRRCGGRGSVVTNPCVICRGSGQTKQKKTVMVPVPAGVEDGQTIRMPVGRKEVFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARSPGLYETINVLIPPGIQAEQRIRMSGKGIPRVNSYGYGDHYLHIKIKVPKRLTDRQRALMLSYAEDEADVEGTVNGIASTTTGGRGTASTGAGEERPEAGDGTEGFLAKLKKMFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPF7</accession>
    <name>A0A6I9YPF7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin and armadillo repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ANKAR</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926041.1">
        <property type="nucleotide sequence ID" value="XM_014070566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPF7"/>
    <dbReference type="GeneID" id="106552323"/>
    <dbReference type="KEGG" id="tsr:106552323"/>
    <dbReference type="CTD" id="150709"/>
    <dbReference type="OrthoDB" id="1683831at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043379">
        <property type="entry name" value="ANKAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46464">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46464:SF1">
        <property type="entry name" value="ANKYRIN AND ARMADILLO REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="352"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="434"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="475"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926041.1"/>
        </source>
    </evidence>
    <sequence length="971" mass="108060" checksum="98EBDC465A486477" modified="2020-10-07" version="1">MLRPIKKQALRNEQADEATYLAHLAAQRNASAFFEKYDRSELQELLTTSFCSWLASKDDMRHPLELPSGILSQMKSVNTSIAIVLMPVEPGLDLDCREIHQMIRELAVGIYCLNQIPSISLDANYDQSTSCSLPPAYFDTRIGQILIAVDYMLKALWHGVYMPKEKRVRFSELWRSTMDIDQDGVPQTLKTPAAEFCQADDKVKTERELPPFLLGTDFKCQHFLYAQDQYFHLHGGIEFDVGTPSIEDVSKEVKAAYDEMHTCAMHHLTQLLDPDAPYREHYPIPVMDFDGKRYYVIGIELEAFYMLFKSQWWGAINEVISTLKPKRLPLSDTQAMEQFKKRFGYRKAIKCWGPTALHLAAQCGSLEALHFLLALRADYQIPDDRGWLPIHFAAYYDNVSCITVLYRKDAQLIEAETQSLCSVLNQRNSPSFSGTIPSLIRLLKSEDIKLECLTLGVLSNISTHESIVRAFVEAGGIPVLIKLLSVEQPELLSRCAVFLYDIAQLDNNQVIIAELHGIPALISLLDNDLQDLLVNVMSCIRVLCKNNKQNQLKVKEMNGIEPLVQFLGSDSDVLVAVASAVIAEICRGNMKMQNAIVAANVIDPLIQLLRGRKISVQVKGAMAIEALCEGNVIIQMRFLAKSVTTFLLKLLKAFHLKVKEQGAVTLWALAGQTLKQQKFMAEQIGYNLIIDMLLSPSDKMQCVGGEAVIALSKDSEIHQNQICEGNGIAPLVRLLRSGRIAEGTLLSVIKALGTMCIGEAHISNPISQENVVDEGALPILVHLLKTHPSLQIKVEVAFSLACIVLKNSSLQTVLQDKEGFSYRDVLKLFHAPDKIVVLAKIIIDVDQVTLSARGISTLVDLLCSEKSSTLVLTGKLLASLAHTRAGIPEAITSLGTVQRLCYHLYAKEEEPGVKWRTTCCPCTHKSCSNEPDGVRIQPTSTPTPTCNIIWLNSLKYSADNTDSSKSGVFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQQ1</accession>
    <name>A0A6I9YQQ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Polyhomeotic-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">LOC106552970</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926853.1">
        <property type="nucleotide sequence ID" value="XM_014071378.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926854.1">
        <property type="nucleotide sequence ID" value="XM_014071379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQQ1"/>
    <dbReference type="GeneID" id="106552970"/>
    <dbReference type="KEGG" id="tsr:106552970"/>
    <dbReference type="OrthoDB" id="2390104at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09577">
        <property type="entry name" value="SAM_Ph1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000011">
        <property type="entry name" value="Polyhomeotic-like protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.160:FF:000002">
        <property type="entry name" value="Polyhomeotic-like protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012313">
        <property type="entry name" value="Znf_FCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038603">
        <property type="entry name" value="Znf_FCS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF86">
        <property type="entry name" value="POLYHOMEOTIC-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16616">
        <property type="entry name" value="PHC2_SAM_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21319">
        <property type="entry name" value="zf-FCS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51024">
        <property type="entry name" value="ZF_FCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FCS-type" evidence="11">
        <location>
            <begin position="97"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="10">
        <location>
            <begin position="260"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013926853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013926854.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="35871" checksum="574860F2A9B1EE21" modified="2020-10-07" version="1">MTSGNGSAPSTVPSALPQNGENKLPPQAIVKPQILTHLIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQAVLAEKPQLQETTTDSEMEDPYLQESKDEASPPKLKCELCGRLDFAYKFKRSKRFCSTACAKRYNVGCTKRVGLFHPDRSKLQKPNTPAHCRRRPCKGALPPLTKESKKTAPASLPPSSAPMAVPSSLQLGHGQEDSSRCSDNSSYEEPLSPISASSSASRRRQGERDMELPDVRLRDLERHFLPSEPTKWNVEDVYEFIHSLPGCQEIADEFRTQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARICMLKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRA9</accession>
    <name>A0A6I9YRA9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Angiomotin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">AMOT</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the angiomotin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926832.1">
        <property type="nucleotide sequence ID" value="XM_014071357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRA9"/>
    <dbReference type="GeneID" id="106552948"/>
    <dbReference type="KEGG" id="tsr:106552948"/>
    <dbReference type="CTD" id="154796"/>
    <dbReference type="OrthoDB" id="5974715at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003365">
        <property type="term" value="P:establishment of cell polarity involved in ameboidal cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009114">
        <property type="entry name" value="Angiomotin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051747">
        <property type="entry name" value="Angiomotin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024646">
        <property type="entry name" value="Angiomotin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826">
        <property type="entry name" value="ANGIOMOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826:SF6">
        <property type="entry name" value="ANGIOMOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12240">
        <property type="entry name" value="Angiomotin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01807">
        <property type="entry name" value="ANGIOMOTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Angiomotin C-terminal" evidence="8">
        <location>
            <begin position="560"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="186"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="309"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="795"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="392"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="619"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="816"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="839"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926832.1"/>
        </source>
    </evidence>
    <sequence length="895" mass="100456" checksum="A666BA21680FFD99" modified="2020-10-07" version="1">MRNSEEPTATTVLQRLLQEQLRYGNPNESRTLLALHQQATLPFASNGSPGAPNEGLSSQDHPIGTHVARQEPQGQEIQMDNCIMEKQLSPRLQTGEDLPTYEEAKVQSQYFRGQPHASVGAAFYVTGVSNQKMRTEGRPTVQRLSPGKVHQDEGLRDLKQGHVRSLSERLMQLSLATSGVKAHAPVTSAPLSPLSSQQTNDFYKTSGAGPPQPGVKGMEHRGPPPEYPYKNVSSPSMGCKPLDPGHFYSDHRLPQQGRSDGPTMRYQHPPEYGSFRQNPEIQLPFQQRPPHHHSPTSSLTSLGSLTLLQSLPPSRLSPSQHQPPLTPSQGDPFSLPRTQPLFLPSQMHQGDIYRFCQPLPGQQQADSYSALPRPPPAPSPYQQMPVDPYVIVSRAQQMVEILSEENKSLQQELDGCYEKVARLQKLETEIQQVSEAYKNLEKSSSKRESLEKTMRNKLECEIRRLHDFNRDLRDRMDTANKQLAEKEFEGTEDNRKTISQLFAQNKETQREKEKLEVELAGFRSTNEEQRRHIEIRDQALNNAQAKVVKLEEELKKKQVYVEKVEKMQQALVQLQAACEKREQLEHRLRTRLERELESLRMQQRQGTSQTANASSEYNAGALMELLREKEERILALEADMTKWEQKYLEESVMRQFALDAAATVAAHRDTTVISPSPNTSYDTSLEARIQKEEEEILLANRRCLDMEGRIKTLHAQIIEKDAMIKVLQQRSRKEASKTDQLSSMRPAKSLISISNAGSGLLSHSSTLSSTPILEDKREEKSWKGSLGVLLGTECRSESIPSTPSPVLPSTPLLSVHSKTGSRDCSTQTDRGSEQSKPPATSPAPPTPGRLPMMNLAYGADKSDGSIFHSSTLERKNPPQNLGQDLKDAEMVEYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTI1</accession>
    <name>A0A6I9YTI1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Orexin receptor type 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106553331</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927279.1">
        <property type="nucleotide sequence ID" value="XM_014071804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTI1"/>
    <dbReference type="GeneID" id="106553331"/>
    <dbReference type="KEGG" id="tsr:106553331"/>
    <dbReference type="OrthoDB" id="10037617at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14993">
        <property type="entry name" value="7tmA_CCKR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF36">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927279.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="37939" checksum="81F6ACE135DDD160" modified="2020-10-07" version="1">MPSLNATRSLLINLAICDLMVVCICMPITTGNLIYKAWVYGDLLCRAAPFIQALSVSASILSLTVISVNRYYSLHNPLRARSFFTHRKILNTILVIWVFSSGICMPLVFTNRRDEIGVGPADDVPLVFPICREVWPREELKQSYNVFLFCALYCLPVSFNVVICFLTVRRLWRPTGTLSGALKENLLASRLKIRRTVAQMVIALVLLFAISWLPFYLVDIWIEFHGPKSLRDEEPSPLALQLRAFSQWLSLTNSSLNPICYCLVGSLYRSAKEMRSRYQKKVASLLSSSPSEKSWLSSLPEMLSHGRSVNSTIKEPDLATRKEGNNGRSHSCRSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUA8</accession>
    <name>A0A6I9YUA8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SULT6B1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013927225.1">
        <property type="nucleotide sequence ID" value="XM_014071750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUA8"/>
    <dbReference type="GeneID" id="106553278"/>
    <dbReference type="KEGG" id="tsr:106553278"/>
    <dbReference type="CTD" id="391365"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="54"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013927225.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="34630" checksum="C540CE613D8A0CE1" modified="2020-10-07" version="1">MTQKKIAEEIEKALEKSKGLNLHELLFSYQGTLYPNTVCSAETFQALENFESRKDDAVLVSYPKCGANWLILIVNDLIATTLKVKQENTELPFMECGDPEKFQRMKQFPSPRIVATHLHYNYLPKSIFKNKAKILVLFRNPKDTAASFFHFHNNAPTIPSYNSWEEFFPEFMNGKVAWGSYFDQAIAWNKHIDDENILILIYEELKEDLVSGVKQIANFFGFPATTEQIQAIADRSTFQAVRDKSQETHGAAGPLLFRKGIVGDWKNIFDKPQNEQMEAKFKECLAGTKLGTKLKYDVYCKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUE6</accession>
    <name>A0A6I9YUE6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transcription factor IIIB 90 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BRF1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927661.1">
        <property type="nucleotide sequence ID" value="XM_014072186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUE6"/>
    <dbReference type="GeneID" id="106553646"/>
    <dbReference type="KEGG" id="tsr:106553646"/>
    <dbReference type="CTD" id="2972"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000002">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.650:FF:000001">
        <property type="entry name" value="transcription factor IIIB 90 kDa subunit isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cyclin-like" evidence="12">
        <location>
            <begin position="51"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="405"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="162"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013927661.1"/>
        </source>
    </evidence>
    <sequence length="451" mass="50885" checksum="E30133D82A22300B" modified="2020-10-07" version="1">MCMLLSSLTSGIDMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHMLEFGDKNHEVSMTALRLLQQMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVIRVVKVGESTLRKRLTEFEDTPASQLTIDEFMTIDLEEECDPPSFTAGQRKIKIQQLEKELSKKLEELEGEITSYQDEIENELENSRPKAKGVYANYSKDDCIEDTISSIFGDEDGEDEELAAAANHLNMDFYNELLDKDRSKEKEISVRPSALESLLGPLPTAASLGITESIKECILTKDRDHNENTGDGELDLSGIDDSEIDMYILNDSEARIKAELWMKENADYLKEQKEKEARIAKEKELGIYKEHKPKKSAKKRELIQASTAGEAIEKMLEQKKISSKINYNVLRDLNSKGGSTPKKDDDTTDDSTSTKKLSRRKSLVNRNVANSVNPVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUJ2</accession>
    <name>A0A6I9YUJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Electron transfer flavoprotein beta subunit lysine methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ETFB lysine methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Protein N-lysine methyltransferase METTL20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">METTL20</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:54193"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. ETFBKMT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927977.1">
        <property type="nucleotide sequence ID" value="XM_014072502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUJ2"/>
    <dbReference type="GeneID" id="106553916"/>
    <dbReference type="KEGG" id="tsr:106553916"/>
    <dbReference type="CTD" id="254013"/>
    <dbReference type="OrthoDB" id="194386at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050078">
        <property type="entry name" value="Ribosomal_L11_MeTrfase_PrmA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43648">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA SUBUNIT LYSINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43648:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA SUBUNIT LYSINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013927977.1"/>
        </source>
    </evidence>
    <sequence length="264" mass="30013" checksum="D0AAD47FA4926E56" modified="2020-10-07" version="1">MVFLGWRRLLHFCGRASFRKDLKQRQRGCLSWRCYRGRISGRLHVDPKLRIFLEENTEIIDAGNLTPEIRLRLLTPNCRFWHDKAALWPYGEPYWAMYWPGGQGLSRYLLDNPKVVQKKKVLDLGSGCGATAIAATLSGASQVVANDIDPVAAAAMVLNCELNNIDPIPVLTENLIGTDIGKWDLIVLGDMFYNEELADSLSEWLKKCIQDDKTGLLIGDPGRPYFVTNRIQRKLHKVSEYTLPQSSPEEYNGSTKTIIWNYQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUQ5</accession>
    <name>A0A6I9YUQ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein DGCR6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gonadal family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928323.1">
        <property type="nucleotide sequence ID" value="XM_014072848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUQ5"/>
    <dbReference type="GeneID" id="106554225"/>
    <dbReference type="KEGG" id="tsr:106554225"/>
    <dbReference type="OrthoDB" id="21617at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010849">
        <property type="entry name" value="Gonadal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13054">
        <property type="entry name" value="DIGEORGE SYNDROME CRITICAL REGION 6 DGCR6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13054:SF2">
        <property type="entry name" value="PROTEIN DGCR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07324">
        <property type="entry name" value="DGCR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928323.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="18450" checksum="600D563FD4185F67" modified="2020-10-07" version="1">MVPSASGRHYSSQQRLSYTILSDLALALLDGTVFEIVQGLLDIQHLMERNLYNQRLKLQTEHRDVEILGKGDNSIPQISKRSQKDAVEQRIHTELRMMDEKIVLELDQKVVDQQSTLEKAGVSGFYITTNPQELMLQMNMLELIRKMQQREMQPRQPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUS3</accession>
    <name>A0A6I9YUS3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Structural maintenance of chromosomes flexible hinge domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106553729</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927766.1">
        <property type="nucleotide sequence ID" value="XM_014072291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUS3"/>
    <dbReference type="GeneID" id="106553729"/>
    <dbReference type="KEGG" id="tsr:106553729"/>
    <dbReference type="OrthoDB" id="10036779at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058611">
        <property type="entry name" value="Ig_SMCHD1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058612">
        <property type="entry name" value="Ig_SMCHD1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058613">
        <property type="entry name" value="Ig_SMCHD1_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058614">
        <property type="entry name" value="Ig_SMCHD1_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038892">
        <property type="entry name" value="SMCHD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22640">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22640:SF2">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26194">
        <property type="entry name" value="Ig_SMCHD1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26195">
        <property type="entry name" value="Ig_SMCHD1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26196">
        <property type="entry name" value="Ig_SMCHD1_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26197">
        <property type="entry name" value="Ig_SMCHD1_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMCHD1 Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="SMCHD1 Ig-like" evidence="2">
        <location>
            <begin position="109"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="SMCHD1 Ig-like" evidence="3">
        <location>
            <begin position="212"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="SMCHD1 Ig-like" evidence="4">
        <location>
            <begin position="319"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013927766.1"/>
        </source>
    </evidence>
    <sequence length="452" mass="50686" checksum="64850E70B1B2C5BE" modified="2020-10-07" version="1">MQIEIMNKKGESIQKLPGPRHGGLNKLLMQLKVVLHSSDKNKELICLISKYGVRWAYWFKKMENIVDLGIYTLTLQVVLNDSHADTYAGIRLPSKKFKFRVREGKPQKFTIGNLETPLRIGHPFNIPLDVQDEFGHTTLLTENIIPVLEASDLTLQYEIKRRPNCAIAGIIAKGLVNNCQGKNFNLKITLPGLKEESQVLKIKLFPGLPQQLNVKLDSDVLKIENGTAFSFPVEVLDVVGNITAQPKLLVQCKFLGVPGLPVYCVDYSSPDKNILTGPVLHIQNLKKVQILEAIIEIPSCKNVSPVNKTIHLVPSSRVAELQINCIEREKRIQIKNEEVITRVAGDVLKNLTFQMYDEGEREIKITPALAKKVKISWTPKLNSKQLIKGLLPDVKVPTSVKDECCCLLTFSDEHVSLASSFVVRPLADKPKHIKCEIKGSDIIKMGEKLQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV14</accession>
    <name>A0A6I9YV14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein FAM155A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106554071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NALF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928142.1">
        <property type="nucleotide sequence ID" value="XM_014072667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV14"/>
    <dbReference type="GeneID" id="106554071"/>
    <dbReference type="KEGG" id="tsr:106554071"/>
    <dbReference type="OrthoDB" id="10047996at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015275">
        <property type="term" value="F:stretch-activated, monoatomic cation-selective, calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055288">
        <property type="entry name" value="NALCN_aux_factor_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819:SF9">
        <property type="entry name" value="NALCN CHANNEL AUXILIARY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN FAM155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928142.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="17488" checksum="B222F9E0568CB675" modified="2020-10-07" version="1">METVYKAWLCSQYFEVTQSHCNNTIPCKQYCLEVQTRCPFILPDNDDVIYGGLSSFICTGLYDNYPTNAEPECCDVRWGLLSDNQSKGTIKTSDSMMCHRTSLTVSSASRLCNSRLKLCVLVLILLHTVLTVSAAQNSTELGFGGITNFEDNSTNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVL5</accession>
    <name>A0A6I9YVL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein Flattop</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cilia- and flagella-associated protein 126</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CFAP126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme. Acts as a regulator of cilium basal body docking and positioning in mono- and multiciliated cells. Regulates basal body docking and cilia formation in multiciliated lung cells. Regulates kinocilium positioning and stereocilia bundle morphogenesis in the inner ear.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Flattop family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928046.1">
        <property type="nucleotide sequence ID" value="XM_014072571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVL5"/>
    <dbReference type="GeneID" id="106553976"/>
    <dbReference type="KEGG" id="tsr:106553976"/>
    <dbReference type="CTD" id="257177"/>
    <dbReference type="OrthoDB" id="521617at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038797">
        <property type="entry name" value="Fltp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34639">
        <property type="entry name" value="PROTEIN FLATTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34639:SF1">
        <property type="entry name" value="PROTEIN FLATTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22611">
        <property type="entry name" value="CFAP126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928046.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="32481" checksum="30EC221D9E8CF9A0" modified="2020-10-07" version="1">MNSWPSQNKKNHKGPKGNVGANAKHRFLLFRQASPWGTYMTTWDMPLKIPPAKVSLTSRSIDAAARLTEWMNKSAALSQACNGLCPEIVGKPSIPVIKEAVSNHPRNSGKTSERPVSSGKMPAFEEVGSRVGATPNGPLSRQPGSMDLRLKDVGGPDAQDAELEAKPPQERNSDDIPLSRQPGCMDLRLKDTSPQLSTSNRPGSREPTPRRTISAEAPPSGRPRSRETRPKGLGTPDHLSPCRPGSLEANPRPGSLQVNPRGDRSPEVLPPSRPLTKSRQGLEVPQTGGAERNAFRPLSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWP2</accession>
    <name>A0A6I9YWP2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dystrobrevin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106554862</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the dystrophin family. Dystrobrevin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929073.1">
        <property type="nucleotide sequence ID" value="XM_014073598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWP2"/>
    <dbReference type="GeneID" id="106554862"/>
    <dbReference type="KEGG" id="tsr:106554862"/>
    <dbReference type="OrthoDB" id="6019271at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16244">
        <property type="entry name" value="EFh_DTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000014">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000016">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000002">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017432">
        <property type="entry name" value="Distrobrevin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF22">
        <property type="entry name" value="DYSTROBREVIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038204">
        <property type="entry name" value="Distrobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="17">
        <location>
            <begin position="238"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="522"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="604"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="550"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013929073.1"/>
        </source>
    </evidence>
    <sequence length="631" mass="71456" checksum="9117EA76656C5C34" modified="2020-10-07" version="1">MIEESGKRRRTMAEKRQLFMEMRAQNFDVIRLSTYRTACKLRFVQKRCNLHLVDIWNMIEAFRDNGLNTLDHNTEINVSRLETIISSIYYQLNKRLPSTHQISVEQSISLLLNFMIAAYDSEGHGKLTVFSIKAMLATMCGGKILDKLRYSFSQMSDSNGLMIFSKFDQFLREVLKLPTAVFEGPSFGYTENSVRACFPQQKKVTLNMFLDTLMADPPPQCLVWLPLMHRLAHVENVFHPVECSYCRCESMMGFRYRCQQCHNYQLCQNCFWRGHANGPHSNQHQMKEHSSWKSPAKKLSHAISKSLGCVPTRDPPHPVYPEQPEKPLDLAHIVPSRPLVNMNDAMVTHVSSGAPTPTKRLQQSLAPSDGLSDEHVLIAAYVSQLQSSTRSVLDSPSRLDEEHRLIARYAARLAAEAGNVTRPPIDLGFNFDANKQQRQLIAELENKNREILQEIQRLRLEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEGLMKLLKEEEQKQAAQAAGSPHTSPTHGAGRSMPMPVRSTSAGSTPTHVPQDSLTGVGGDVQEAFAQGTRRNLRNDLLVAADSITNTMSYLVKELHSGGEEGGEEEEEEDKMQNGKDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXB3</accession>
    <name>A0A6I9YXB3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sperm-associated antigen 16 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106554730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928937.1">
        <property type="nucleotide sequence ID" value="XM_014073462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXB3"/>
    <dbReference type="GeneID" id="106554730"/>
    <dbReference type="KEGG" id="tsr:106554730"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990716">
        <property type="term" value="C:axonemal central apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002905">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050995">
        <property type="entry name" value="WD-F-box_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF3">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="96"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="138"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013928937.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="19967" checksum="C37BCF43CAFBA8B3" modified="2020-10-07" version="1">MILIDLGDDQAVGHWCDREAGLVGPSQRALREAKEQKVHSAVNARDSTKDTAKQMSKKYPKDSEFPEGIQVNPYLACAKKCVRPLKSGVYKLCNTLKVHDLAVTCLALHPRKDIVVTGSDDRLWKMWAFPNGNIIVKGEGHTDWLSGCCFHPGGCKLVTSSGDKTVRIWDFTKSESILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXJ9</accession>
    <name>A0A6I9YXJ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Butyrophilin subfamily 1 member A1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106554235</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Neurotoxin that produces dose-dependent hypolocomotion and hyperalgesia in mice. May directly act on the central nervous system, as it is 6500-fold more potent when administered intracerebroventricularly than intraperitoneal.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ohanin/vespryn family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928335.1">
        <property type="nucleotide sequence ID" value="XM_014072860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXJ9"/>
    <dbReference type="GeneID" id="106554235"/>
    <dbReference type="KEGG" id="tsr:106554235"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd12888">
        <property type="entry name" value="SPRY_PRY_TRIM7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0528">Neurotoxin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0800">Toxin</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="40"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013928335.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="26198" checksum="1D5E4F2216AE5E7D" modified="2020-10-07" version="1">MENFKKFRESLMDELSKSKTRGLNAVLLSQTAQPDQPQIFWPRLYNDAVRADKKVSPVSVTLDPRTANPHLLLGSDRKTVTRLDHRQTLPDNPERFDAEFFVLGCESFCSGKHSWVVNVEQGQNWAIGIARESVRRKGSLSLSPQQGIWAVQQCWGQLQALTSRWTTLFLLRKPKRIRVHLDYEGGWVAFFDAELDAPIFTFPPTSFKRERIHPWFWVASGSQLTLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXT9</accession>
    <name>A0A6I9YXT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Complement decay-accelerating factor transmembrane isoform-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106554305</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928430.1">
        <property type="nucleotide sequence ID" value="XM_014072955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXT9"/>
    <dbReference type="GeneID" id="106554305"/>
    <dbReference type="KEGG" id="tsr:106554305"/>
    <dbReference type="OrthoDB" id="406096at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF570">
        <property type="entry name" value="COMPLEMENT COMPONENT 4 BINDING PROTEIN, MEMBRANE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027027599" evidence="6">
        <location>
            <begin position="33"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="32"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="158"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="223"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="225"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013928430.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="34350" checksum="0F45AAD0A66305E9" modified="2020-10-07" version="1">MSRSSPAMIGNFFSHLGPRILVLFSLFSGVLGDCGPPPRLKNAEPFDPIKPSYKPYEYVVFKCLPGYREDSSKPYMTTCSPDKGWNPLEEFCERGCKNPRETRFSVVVEFLRFYPVGTVVHHVCHRSAIHIPGRPERPAITCLSNYTWSSVPVFCKGLPCGDPGKPENGDRIMLMDFLLKAKVNFSCNEGYQLIGSPTIQCLSRAYDNNTVVWKPKPPICFRPTCPPPPEIKNGIYTGGKNVTFPLYSNVTYTCDLGFSLSGGDLLHCITEDNKTGIWRGSVPECKDNMGSFHKETYFGVLFFFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYK0</accession>
    <name>A0A6I9YYK0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger CCCH domain-containing protein 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZC3H15</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ZC3H15/TMA46 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929124.1">
        <property type="nucleotide sequence ID" value="XM_014073649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYK0"/>
    <dbReference type="GeneID" id="106554905"/>
    <dbReference type="KEGG" id="tsr:106554905"/>
    <dbReference type="CTD" id="55854"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000050">
        <property type="entry name" value="AGAP008634-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="176"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="176"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="360"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="220"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013929124.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="48794" checksum="E2A4838372ACB066" modified="2020-10-07" version="1">MPPKKPPPAAAAGNKKADQKKKEKIIEDKTFGLKNKKGAKQQKFIKAVTHQVKFGQQNPRQAAQVDGDKKLKKDDKKKELQELNELFKPVVAAQKISKGADPKSVVCAFFKQGQCTKGDKCKFSHDLSLERKCEKRSVYIDARDEELEKDTMDNWDEKKLEEVVNKKHGEAEKKKPKTQIVCKYFLDAIESNKYGWFWVCPGGGDNCMYRHALPPGFVLKKDKKKEEKEEEISLEDLIEKERSLLGSNVTKITLESFLAWKKRKRQEKIDKAEQDMERRKADFKAGKALVISGREVFEFRPELVDADDEEADDTHYIQGAEEDEVDELVRVNDIDLNPYVPKDVDETGITVASLERFSTYASTEKDDNKLSEASGGGLENGEQSDLDPDSDMEGNPENGVIDAVPVDENLFTGEDLEELEEELNTLDLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0C1</accession>
    <name>A0A6I9Z0C1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2 11">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106555419</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="2 11"/>
    <dbReference type="RefSeq" id="XP_013929739.1">
        <property type="nucleotide sequence ID" value="XM_014074264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0C1"/>
    <dbReference type="GeneID" id="106555419"/>
    <dbReference type="KEGG" id="tsr:106555419"/>
    <dbReference type="OrthoDB" id="9041617at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000033">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-79 specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="13">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="280"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929739.1"/>
        </source>
    </evidence>
    <sequence length="405" mass="46510" checksum="ED28D864A421AEB4" modified="2020-10-07" version="1">MENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDKEFRKWMKWYGKKHADYTLERGDFLSEEWRDRIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGQIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNTTTPTKVQENKDDGAEEDKNLAANTVKKPSPSKARKKKLSKKGRKMAGRKRGRPKKMNVVNADRKIKKNQTALELLHAQTVSQTNSSSPQDAYRSPHSPFYQLPPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0R4</accession>
    <name>A0A6I9Z0R4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein regulator of cytokinesis 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PRC1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929889.1">
        <property type="nucleotide sequence ID" value="XM_014074414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0R4"/>
    <dbReference type="GeneID" id="106555546"/>
    <dbReference type="KEGG" id="tsr:106555546"/>
    <dbReference type="CTD" id="9055"/>
    <dbReference type="OrthoDB" id="642895at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF1">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013929889.1"/>
        </source>
    </evidence>
    <sequence length="437" mass="50589" checksum="F27133C3B0419987" modified="2020-10-07" version="1">MTGPAEVLAGESVACLTQALSHLRGIWEEIGIPEEQRLQRTEVVKRHVKDLLEQMVAEEESLRERLLKSIALCRRELGALCQELQLAPFQEEEEATILQLEKDLRTRVEVMLKQKRERRQELRALQERDQELAGLLGRAPYNIGSSEAVPSLADLDCFRRHLASLAAEKEHRQAEFVHLKQKVILCMEELEQEPSTTFEREAMCQDSGTFCFSLNNLAALKDLLQQLEERRAQKEAVCEELRCRILMLWDWLQVPPEERNAFAPYMEGSRASIMDALHLEVDRLEELKCQNLQKVVEAIRAELATYWDKCFLGEEQRCSFGPYYEDTFSEELLQQHDNELMRLKHYYETHQELFEAIHKWKRSWCLFQELEKKATDLSRFTNRGGNLLKEEKLRAKLQKTLPKASGPSHSPYPVNLWQPHLNLTFSSAVGGGAEGPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z189</accession>
    <name>A0A6I9Z189_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Acetylcholine receptor subunit epsilon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CHRNE</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Epsilon/CHRNE sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930773.1">
        <property type="nucleotide sequence ID" value="XM_014075298.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106556301"/>
    <dbReference type="KEGG" id="tsr:106556301"/>
    <dbReference type="CTD" id="1145"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000048">
        <property type="entry name" value="Cholinergic receptor nicotinic epsilon subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000010">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026950178" description="Acetylcholine receptor subunit epsilon" evidence="17">
        <location>
            <begin position="24"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="18">
        <location>
            <begin position="32"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="19">
        <location>
            <begin position="281"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013930773.1"/>
        </source>
    </evidence>
    <sequence length="537" mass="60550" checksum="688D5548CE4BE457" modified="2020-10-07" version="1">MQAVGSLARGFCLGLLLGWVALGSPEGNKELEIFNRLFTGYDKSRWPVQGVDEVLTVSLKLTLTNLISLNAKEETLTTNVWITQEWLDYRLNFSSEEYGQSLRVPSDLVWLPDIVLENNIDGNFEVAYSAXWHPMPTDQLRRHITVPSVFTGETAATYLRSLFTYERGGHVVLIPEYIFPLGYYLIPFTGVVGVVIAVMCTILIVRCVQHRKRMRKDRLSKEQLKKIPLHKYRKENGEWAIKKRPARRVLAPVAGLEAPGFSEIHYILIIQRKPLFYVINIIVPCVLISSLVVLVYFLPAQAGGQKCTVSISVLLAQTVFLFLISQKVPETSLSVPLIGKYLLFVMTVATLIVTNCIVVLNVSLRSPSTHRLSPRLKHLFLKFLPHYLGSNIGPADEDESLGAPCPRRSFSTLVKAEEYILKKARSEVLFEHQGQRHGLQRAPHYDGLDVEMTTMLYKSLSSLAPEIQECVDACNFISKSTKEQNKDGAEIENWMLIGQMIDKLCFWLALLLFTIGTLAIFFMGHLNSAPEDPFPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2D4</accession>
    <name>A0A6I9Z2D4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Short-chain dehydrogenase/reductase 3</fullName>
            <ecNumber evidence="3">1.1.1.300</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106555968</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>all-trans-retinol + NADP(+) = all-trans-retinal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25033"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.300"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.300" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013930389.1">
        <property type="nucleotide sequence ID" value="XM_014074914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2D4"/>
    <dbReference type="GeneID" id="106555968"/>
    <dbReference type="KEGG" id="tsr:106555968"/>
    <dbReference type="OrthoDB" id="6251714at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004745">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048385">
        <property type="term" value="P:regulation of retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF483">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013930389.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31826" checksum="7098BE98C4EE33B4" modified="2020-10-07" version="1">MGTEGHYFVCDVANREEVYRQAKAVREKVGDVTILVNNAAVVHGKTLLDSDDHALLKSQHINTLGQFWTTKAFLPRMLELQRGHIVCLNSVLALSAIPGAVDYCTSKAASFAFMESLTLGLLDCPGVNATTVLPFHTNTEMFQGIRIRFPNLFPPLKPETVARRTVEAVQQKQAFLLLPWTMHILIFLKRYLSGKMVPSEKWNPEVTMANNNQQGRGMGGNKPTGVADPWKEASHALVEDQWKRRRRGLLSRRWRLFQVAPVKEELLGLENELWGGGCPGQPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3T3</accession>
    <name>A0A6I9Z3T3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Apolipoprotein A-I-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106556173</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930626.1">
        <property type="nucleotide sequence ID" value="XM_014075151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3T3"/>
    <dbReference type="GeneID" id="106556173"/>
    <dbReference type="KEGG" id="tsr:106556173"/>
    <dbReference type="OrthoDB" id="8727817at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042627">
        <property type="term" value="C:chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034362">
        <property type="term" value="C:low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060228">
        <property type="term" value="F:phosphatidylcholine-sterol O-acyltransferase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055090">
        <property type="term" value="P:acylglycerol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033700">
        <property type="term" value="P:phospholipid efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050163">
        <property type="entry name" value="Apolipoprotein_A1/A4/E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18976">
        <property type="entry name" value="APOLIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18976:SF11">
        <property type="entry name" value="APOLIPOPROTEIN A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0345">HDL</keyword>
    <keyword evidence="4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026731317" evidence="5">
        <location>
            <begin position="19"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013930626.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29879" checksum="47E4ACCBD00EE84F" modified="2020-10-07" version="1">MQSLNLLLALLLLTGAQARYFWQHDDPQQTRLERLQELGQVYLESAKVAVQEAVGQFDSSAVAQELQVSLGQKLEAVTIVLRQIKEEIAPELEEVRNELRAAWQKLKEEVINDVQDVGVQIQPFLRKFRENVQQDAAIYFQQLKTISQDINQAAQDKLRPVAEAFRDKVRSHVDAFRKDAAPYTKKLEQLLQEKAKTLEQSALTELTELQTELQQQLSEAREKYGPLLEKAREELLTLVGSAKSFFVTDSGSEQEVQQQESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3Y7</accession>
    <name>A0A6I9Z3Y7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106557171</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013931808.1">
        <property type="nucleotide sequence ID" value="XM_014076333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3Y7"/>
    <dbReference type="GeneID" id="106557171"/>
    <dbReference type="KEGG" id="tsr:106557171"/>
    <dbReference type="OrthoDB" id="336747at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000002">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000001">
        <property type="entry name" value="calcium/calmodulin-dependent protein kinase type II subunit gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013931808.1"/>
        </source>
    </evidence>
    <sequence length="478" mass="54141" checksum="01B6863DE039DA24" modified="2020-10-07" version="1">MASTATCTRFTDEYQLFEELGKGAFSVVRRCLKITTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHESISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRINATEALKHPWICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESTNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHRFYFENALSKSNKPVHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWHRRDGKWQNVHFHRSGSPTVPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z440</accession>
    <name>A0A6I9Z440_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7 11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">UCHL5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 10 11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7 11"/>
    <dbReference type="RefSeq" id="XP_013931868.1">
        <property type="nucleotide sequence ID" value="XM_014076393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z440"/>
    <dbReference type="GeneID" id="106557221"/>
    <dbReference type="KEGG" id="tsr:106557221"/>
    <dbReference type="CTD" id="51377"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02255">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.860:FF:000001">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000001">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033837">
        <property type="entry name" value="UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52049">
        <property type="entry name" value="ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="13">
        <location>
            <begin position="9"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="223"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 10">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="9 10">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013931868.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="37564" checksum="F664F3E7607B89CE" modified="2020-10-07" version="1">MAGGGSAGEWCLMESDPGVFTELIKGFGCRGAQVEEIWSLEPENFEKLKPVHGLIFLFKWQPGEEPAGSVVQDSRLDTLFFAKQVINNACATQAIVSVLLNCTHQDIHLGETLSEFKEFSQSFDAAMKGLALSNSEVIRQVHNSFARQQMFEFDAKSTAKEEDAFHFVSYVPVNGRLYELDGLREGPIDLGVCNQDDWINAVRPVIEKRIQKYSEGEIRFNLMAIVSDRKMIYQQKIANLQRQLAEEEPMDTDQSCISSVQSEVAKYQMLIEEENQKLKRYKIENIRRKHNYLPFIMELLKTLAEHQQLIPLVEKAKEKQNAKKPQEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z8J6</accession>
    <name>A0A6I9Z8J6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methylmalonyl-CoA mutase, mitochondrial</fullName>
            <ecNumber evidence="4">5.4.99.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methylmalonyl-CoA isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MUT</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-methylmalonyl-CoA = succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:22888"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57326"/>
            <dbReference type="EC" id="5.4.99.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:22889"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Interacts (the apoenzyme form) with MMAA; the interaction is GTP dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylmalonyl-CoA mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013932100.1">
        <property type="nucleotide sequence ID" value="XM_014076625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z8J6"/>
    <dbReference type="GeneID" id="106557392"/>
    <dbReference type="KEGG" id="tsr:106557392"/>
    <dbReference type="CTD" id="4594"/>
    <dbReference type="OrthoDB" id="198977at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019678">
        <property type="term" value="P:propionate metabolic process, methylmalonyl pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02071">
        <property type="entry name" value="MM_CoA_mut_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03679">
        <property type="entry name" value="MM_CoA_mutase_alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.240:FF:000002">
        <property type="entry name" value="Methylmalonyl-CoA mutase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.280:FF:000002">
        <property type="entry name" value="Methylmalonyl-CoA mutase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.240">
        <property type="entry name" value="Methylmalonyl-CoA mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058549">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006944">
        <property type="entry name" value="PRK09426.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF4">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="Cobalamin (vitamin B12)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00544">
        <property type="entry name" value="METMALONYL_COA_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="11" id="KW-0170">Cobalt</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="B12-binding" evidence="16">
        <location>
            <begin position="612"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013932100.1"/>
        </source>
    </evidence>
    <sequence length="748" mass="82950" checksum="6C43008E425A8A03" modified="2020-10-07" version="1">MLRVKNGLLRHWPLCCKREASLFPLQPSWCLLHQHVLHPEWTAMAKKQLKGKSPEELIWHTPEGIAIKPLYSTRDTAGFPEELPGVKPFTRGPYPTMYTYRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILFDGIPLEKMSVSMTMNGAVIPILATFIVTGEEQGVPQSKLTGTIQNDILKEFMVRNTYIFPPEPSMQIIADIFQYTSKHMPKFNSISISGYHMQEAGADAILELAYTIADGLEYCRTGLKAGLTIDEFAPRLSFFWGIGMNFYMEIAKLRAGRRLWAHLIEKMFQPKDTKSLLLRAHCQTSGWSLTEQDPYNNIIRTTVEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSFLMESLTNDVYEAALKLINEIEEMGGMAKAVAEGIPKLHIEECAARRQARIDSGSEVIVGVNKYQLEKEETVEVLAIDNTSVRNKQIEKLIKVKACRDEVAAQKCLTALTECAATRQGNLLALAVDAARARCTVGEITDAMKKVFGEHRASDRMVSGAYRQEFGESDEITQAISRVNKFLEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPLEVAQQAVDADVHCVGVSTLAAGHKTLVPELIKQLNYLGRPDILVMCGGVIPPQDYDYLYETGVSNIFGPGTRIPKAAVQVLDDIEKCLDKRQQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-09-12" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2U8BQD4</accession>
    <name>A0A2U8BQD4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">G protein gamma subunit 2</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="13">Guanine nucleotide-binding protein G(T) subunit alpha-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">GNAT2</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2018" name="Mol. Biol. Evol." volume="35" first="1376" last="1389">
            <title>Shifts in Selective Pressures on Snake Phototransduction Genes Associated with Photoreceptor Transmutation and Dim-Light Ancestry.</title>
            <authorList>
                <person name="Schott R.K."/>
                <person name="Van Nynatten A."/>
                <person name="Card D.C."/>
                <person name="Castoe T.A."/>
                <person name="S W Chang B."/>
            </authorList>
            <dbReference type="PubMed" id="29800394"/>
            <dbReference type="DOI" id="10.1093/molbev/msy025"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="MH011538">
        <property type="protein sequence ID" value="AWC68172.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931475.1">
        <property type="nucleotide sequence ID" value="XM_014076000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2U8BQD4"/>
    <dbReference type="GeneID" id="106556951"/>
    <dbReference type="KEGG" id="tsr:106556951"/>
    <dbReference type="CTD" id="2780"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001580">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of bitter taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000720">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000256">
        <property type="entry name" value="Guanine nucleotide-binding protein G(t) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF68">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AWC68172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013931475.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="40075" checksum="CCF1805C1006DD84" modified="2018-09-12" version="1">MGSGASAEDKELAKRSKELEKKLQEDGDKEAKTVKLLLLGAGESGKSTIVKQMKIIHQDGYTEEECLEFKAIIYSNILQSILSIIRAMSTLGIDFADSGRADEQRQLYSLADSIEEGTMPPELTTCIKKLWADGGVQACFDRAAEYQLNDSASYYLNQLDRITAPGYLPNEQDVLRSRVKTTGIIETKFSVKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCGALSAYDMVLVEDDDMNRMHESLHLFNSICNHKFFAATSIILFLNKKDLFEEKIKKVHLSICFPDYDGPNTFEDAGNYIKLQFLDLNMRKDAKEIYSHMTCATDTQNVKFVFDAVTDVIIKENLKDCGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYA1</accession>
    <name>A0A6I9WYA1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TSPAN14</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907136.1">
        <property type="nucleotide sequence ID" value="XM_014051661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYA1"/>
    <dbReference type="GeneID" id="106537507"/>
    <dbReference type="KEGG" id="tsr:106537507"/>
    <dbReference type="CTD" id="81619"/>
    <dbReference type="OrthoDB" id="2014092at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03159">
        <property type="entry name" value="TM4SF9_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000001">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF261">
        <property type="entry name" value="TETRASPANIN-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013907136.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="30921" checksum="E5DDAD5753747CF6" modified="2020-10-07" version="1">MHYYRYPSAEISCWYKYLLFSYNIIFWLAGVAFLGAGLWAWSEKGVLSDLSKMTRLHGFDPVFLILFVGGVMFILGFAGCVGALRENICLLKFFCGTIVVIFILELALAVLAFLFQDWVKDRVETFFKNNIKAYRDDIDLQNLIDSLQKMNQCCGAQSPNDWNLNIYFNCSSASKSRERCGVPFSCCIPDPAQNVVNTQCGYDVRNMSLSYWEERIFTRGCIAALEAWLPRNIYIVAGAFIAISLLQIFGIFLAKTLISDIDAVKSGFYFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYQ4</accession>
    <name>A0A6I9WYQ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protocadherin gamma-C3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106537617</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907276.1">
        <property type="nucleotide sequence ID" value="XM_014051801.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106537617"/>
    <dbReference type="KEGG" id="tsr:106537617"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000185">
        <property type="entry name" value="Protocadherin 2 alpha c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF119">
        <property type="entry name" value="PROTOCADHERIN ALPHA-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026980713" description="Protocadherin gamma-C3" evidence="13">
        <location>
            <begin position="20"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="59"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="168"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="284"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="374"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="491"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013907276.1"/>
        </source>
    </evidence>
    <sequence length="687" mass="76162" checksum="964E71A392C71357" modified="2020-10-07" version="1">MRRLILLLPQLLHLGLAAGQLVYQVREELLLELVVKQPVHVHDGKVEVLDINDNVPRFPRQEYSLEISESAMPGSCFLMESLQDPDVGSNSVKXYQLTPSEHFGLDLQNLKPGSKLLELVLLQPLDREQSTHQQLMLVALDGDNPAKSATAQISVGVLDINGNSPEFDLSIYMTTLLENIEPGMLVVKLKALDPDEGSNGDLLYSFSSYTPQKVCQLFTVHPVSGEMRVNGSLDYEEDISYEIYVQATDKGSVPMIGHCKVLINIVDANDNAPEVVLSSLYSPVSEDAPVGTVVAVLNVIDLDSGLNRQVSINIPANLPFKLNSFKNSYTLVTEDXSSYNITITATDAGTPPLSSHKIIKIDVFDINDNSPQFEELSYSIYIPENNAPGSTLCTTKGIDCDANANACISYTLIMEEIQGLPASFDVSIYAVRSFDYETLRKFQIVVQAKDSGMPSLSSTATVHIYVLDQNDHVPQTFYPISMNNLTAIEMIPDSASSGYLITKVIAIDGDSGQNAWLFFHLAQTSVPNIFRVEVHSGEIWTTEKLREATEATFNLTIIVRDNAEPPLSSSMSVIVVTIKKLNPTASKKLLKPPNVWLNNYEGNRIQVLGRMEKVTIAELLHKLLGKIALWTWIQKEDWAFAAVKELLSSNSMLIQFNHTFPVWITCDASLELEWSSAITYWLDQRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0H1</accession>
    <name>A0A6I9X0H1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA-binding protein 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RBM4B</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013907856.1">
        <property type="nucleotide sequence ID" value="XM_014052381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0H1"/>
    <dbReference type="GeneID" id="106538018"/>
    <dbReference type="KEGG" id="tsr:106538018"/>
    <dbReference type="CTD" id="83759"/>
    <dbReference type="OrthoDB" id="79941at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000085">
        <property type="entry name" value="RNA-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013907856.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="30169" checksum="A8CA29581E4CA294" modified="2020-10-07" version="1">VGNISTSCTNLELRSKFEEYGPVLECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGKRMRVQLSTSRLRTAPGMGEKSGCYRCGKEGHWSKECPVDRTGQAAELTEPYSEQYGMVRTPYPTGYGESMYYDDPYGMVDYYKRYRARNAAAYGASAYDTYAEHTMAQYSQYAQYSQATAMASRLSTSLDPYERPLMPSSGSAAVAVAAVAATSSFYTRDRSPLRRSAAAASTAGDVYGYERVQLSPVSRSSLYDVQRFGRDAGADRLRYSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3K8</accession>
    <name>A0A6I9X3K8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytosolic phospholipase A2 gamma-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106539592</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909874.1">
        <property type="nucleotide sequence ID" value="XM_014054399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3K8"/>
    <dbReference type="GeneID" id="106539592"/>
    <dbReference type="KEGG" id="tsr:106539592"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF39">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2 3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="4">
        <location>
            <begin position="3"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013909874.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="63417" checksum="B50D2BC0B804A8B1" modified="2020-10-07" version="1">MDMTGKGKTKVRISKSLSDGEEVAITARKKKVQKALAKLGMQCDSENIPNIAVLGAGGAMRAMIALYGTLAELKKYNLLDCVMYLGGVSGSTWSLPTLYKNEDWSEEIENVIKCQIENLVQGQWDVNKAIKAVLEATEDECYSLTDFWSYFLVHKLLSKFEEGKLSEHAGSCENGKNPYPIYAAVNKQTYQNPNAGTWFEFTPHEVGIPGLGTYVDSKYFGSIFQNGQLIREKKGKNICYLQGLWGSALGGEQEISKTISDALKDFGKCEKSEDITSKDLADIKKFNMLYECYQTLLELQLLESTDKEGLDKFDHLENILKDYKYSKSYQLIREMRETWYSADEQRKKEQYTTLFQTLDADFGDFTNEFNKRCLKMVKKTCLCLLNWSWGSIHNFLYQCTDVKFPELTSNPIVSLIDAGLTINTAYPSVLRPERQVKLILSFDFSAGDPFLTIKKAAEYCEAHAIPFPKIDENALQDLDNPLDCYIFRGEHTPTIIHCPLFNKINCPGEIAEYREEFSTFKLNYSVEEIEKLLIAAKKNVANVQQKILEEINHIVGSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4A6</accession>
    <name>A0A6I9X4A6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106539801</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013910169.1">
        <property type="nucleotide sequence ID" value="XM_014054694.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539801"/>
    <dbReference type="KEGG" id="tsr:106539801"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008349">
        <property type="entry name" value="MAPK_ERK1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01770">
        <property type="entry name" value="ERK1ERK2MAPK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="109"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910169.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="36577" checksum="853469DAA38A9F99" modified="2020-10-07" version="1">MRKQPDTKERGQPDQRSSGRREAAAPQLAPTNSPTKRAEMLQMRRDMPHGIGLCDPSPETQAIATTQGTTGMRPQTCRRYIPKSPQGCRSHSPPSRRGWGTPANDDQSPNPYDSKDEGENDPMTSRKLNQCXYDHVNKIRAAIKKISPFEHQTYCQRTLREIKILLRFKHENVIGINDILRAPTIDQMRDVYIVQDLMETDLYKLLKTQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4B5</accession>
    <name>A0A6I9X4B5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Proteinase-activated receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Thrombin receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106539008</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909151.1">
        <property type="nucleotide sequence ID" value="XM_014053676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4B5"/>
    <dbReference type="GeneID" id="106539008"/>
    <dbReference type="KEGG" id="tsr:106539008"/>
    <dbReference type="OrthoDB" id="8881832at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015057">
        <property type="term" value="F:thrombin-activated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030194">
        <property type="term" value="P:positive regulation of blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15369">
        <property type="entry name" value="7tmA_PAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000040">
        <property type="entry name" value="Coagulation factor 2 (thrombin) receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003912">
        <property type="entry name" value="Protea_act_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000935">
        <property type="entry name" value="Thrmbn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF20">
        <property type="entry name" value="PROTEINASE-ACTIVATED RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01428">
        <property type="entry name" value="PROTEASEAR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00908">
        <property type="entry name" value="THROMBINR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="14 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027006067" description="Proteinase-activated receptor 1" evidence="19">
        <location>
            <begin position="18"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="269"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="20">
        <location>
            <begin position="119"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="175"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013909151.1"/>
        </source>
    </evidence>
    <sequence length="428" mass="48112" checksum="1CA864A5C9352560" modified="2020-10-07" version="1">MGPPLVLVALGWIAVLALPLHGFSPARNGSSFRNDKESILRTFPMGEQKEKYEQIPVLDNDAENDSEIGSGSISHSRTLPRPLPKYVLREGVEDYLRSGWLTYFVPGVYTLVASLSLPLNITAIFLFLLKMKVKKPAVVYMLNLAFADVLFACVLPFRIVYHFSGNDWPFGPAMCRFVTATFYCNMYCSILLMTVISIDRFLAVVYPMQSLSWRTLRRASVVCVAIWLVSIAAVVPLFITEQTRNILPLNITTCHDVLNESDLQAYYRIFFTIFFSFFFFVPLIVCTVCYVCIICCLSSSNIAIKSGKRGRALLLSVAVLSIFIICFGPTNLLLLVHYAHFAYSNYTGNIYFAYLLSVCISSVSCCIDPLIYYYASSECQRQLSNLLCCRKDSELCSLTSDNPLGSRISQRTTGTSTMDNSLYRKLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5I1</accession>
    <name>A0A6I9X5I1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">5' exonuclease Apollo</fullName>
            <ecNumber evidence="5">3.5.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DNA cross-link repair 1B protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">SNM1 homolog B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DCLRE1B</name>
        <name evidence="20" type="synonym">LOC106540534</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-lactam + H2O = a substituted beta-amino acid</text>
            <dbReference type="Rhea" id="RHEA:20401"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35627"/>
            <dbReference type="ChEBI" id="CHEBI:140347"/>
            <dbReference type="EC" id="3.5.2.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.6" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013909526.1">
        <property type="nucleotide sequence ID" value="XM_014054051.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911164.1">
        <property type="nucleotide sequence ID" value="XM_014055689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5I1"/>
    <dbReference type="GeneID" id="106539288"/>
    <dbReference type="GeneID" id="106540534"/>
    <dbReference type="KEGG" id="tsr:106539288"/>
    <dbReference type="KEGG" id="tsr:106540534"/>
    <dbReference type="CTD" id="64858"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000003">
        <property type="entry name" value="DNA cross-link repair 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF26">
        <property type="entry name" value="5' EXONUCLEASE APOLLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10 19" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="17">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013909526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013911164.1"/>
        </source>
    </evidence>
    <sequence length="555" mass="63285" checksum="73549D5E612391DA" modified="2020-10-07" version="1">MNGTLIAGTPIAVDFWNIRKARQARLFFLSHMHSDHTVGLSSTWNQPVYCSPVTGQILHLRLKVAKQLIRPLEVGASHVVALDDIGKETMTVTLIDANHCPGSVMFLFEGYFGVILYTGDFRYAPNMQQDPVLRNSKLINCLYLDNTNCRPNTILPSREQATEQIKKLIRDHPDHRVKIGTYSLGKESLLVELAQEFHTWIVVSHQKLELLQLLEIEDVFTSEKGAGWIHAVDFSEICKETITNWNRSHPTIAILPTSRPVKISHPNMHVVPYSDHSSFQELLEFVAWLKPCSIIPVVKNDLCQVYFQQYLSSENYSLLESKIPESVKQAMKNKKKERPIKLLKLSISHHAPRGVCFDSPEKCTEQNEYFPETEISEQCCPESTNGTVPYSRQEQCAQFPQCEEKQEETIIEPSNLENDRVLTVVESENMFSTKHQLKDNADNECKTCHIAVTLACTCTLSNDRKDTISTLERVPSSCEKSDCLPQPAMDNDVFSTASQTDQIHRVANQHVLKNLSREYDLSPLNSSKRNSLKNFDLQVENYIKRRRVSRSIELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X696</accession>
    <name>A0A6I9X696_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Thrombospondin-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106539497</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909771.1">
        <property type="nucleotide sequence ID" value="XM_014054296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X696"/>
    <dbReference type="GeneID" id="106539497"/>
    <dbReference type="KEGG" id="tsr:106539497"/>
    <dbReference type="OrthoDB" id="14563at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000001">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000002">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017897">
        <property type="entry name" value="Thrombospondin_3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008859">
        <property type="entry name" value="Thrombospondin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028974">
        <property type="entry name" value="TSP_type-3_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199:SF88">
        <property type="entry name" value="CARTILAGE OLIGOMERIC MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199">
        <property type="entry name" value="THROMBOSPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02412">
        <property type="entry name" value="TSP_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05735">
        <property type="entry name" value="TSP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103647">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51234">
        <property type="entry name" value="TSP3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51236">
        <property type="entry name" value="TSP_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="repeat" description="TSP type-3" evidence="6">
        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="TSP type-3" evidence="6">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="TSP type-3" evidence="6">
        <location>
            <begin position="253"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="TSP C-terminal" evidence="8">
        <location>
            <begin position="316"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013909771.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="48427" checksum="8026B24BE89DC8CD" modified="2020-10-07" version="1">MATTIEARNLNMAAADNVAHRLSALWMPSMGSVLSTSEQPIIRKTDNCVTVPNSGQEDADRDGIGDACDDDADGDGIKNSEDNCVFVPNADQQNVDLDNFGDACDNCRNIKNNNQWDSDGDGWGDLCDNDMDGDKIKNVLDNCPRIPNSDQKDTDGDGVGDVCDSCPFISNPDQKDTDHDLVGDPCDTNQDKDGDGHQDSRDNCPSVPNSSQLDSDKDGLGDECDDDDDNDGIPDHNPPGPDNCRLVPNPGQEDTNRDGVGDACQDDFDKDHVVDRIDVCPENAQVTLTDFRAFQTVVLDPEGDAQIDPNWIVLNQAVKSKTGPGEYLRNSLWHTGDTVDQVKLLWKDPRNVGWKDKTSYRWFLQHRPQVGYIRTRFYEGSEMVADTGIVLDTTMRGGRLGVFCFSQENIIWSNLRYRCNDTIPEDYEAYRNQHDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7L7</accession>
    <name>A0A6I9X7L7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein FAM180A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FAM180A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910256.1">
        <property type="nucleotide sequence ID" value="XM_014054781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7L7"/>
    <dbReference type="GeneID" id="106539868"/>
    <dbReference type="KEGG" id="tsr:106539868"/>
    <dbReference type="CTD" id="389558"/>
    <dbReference type="OrthoDB" id="8913792at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029170">
        <property type="entry name" value="FAM180"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34034:SF2">
        <property type="entry name" value="PROTEIN FAM180A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34034">
        <property type="entry name" value="PROTEIN FAM180A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15173">
        <property type="entry name" value="FAM180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013910256.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="14494" checksum="0BA199EC14AEF089" modified="2020-10-07" version="1">MNNLKLHGKFLLGELEIDDDLKITVKDEELASTRKAIMFDTLCNDVIPKSITEIRRLSSRLAGYPGMLKKEDFERTVLTMVYTAYRTAQSQAHQKENWAESFVNLYKLVMHDLKLLSSQAPTSQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDH2</accession>
    <name>A0A6I9XDH2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Integrin alpha-6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ITGA6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913679.1">
        <property type="nucleotide sequence ID" value="XM_014058204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDH2"/>
    <dbReference type="GeneID" id="106542467"/>
    <dbReference type="KEGG" id="tsr:106542467"/>
    <dbReference type="CTD" id="3655"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050900">
        <property type="term" value="P:leukocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000002">
        <property type="entry name" value="integrin alpha-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000002">
        <property type="entry name" value="integrin alpha-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1530:FF:000001">
        <property type="entry name" value="Integrin subunit alpha 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF9">
        <property type="entry name" value="INTEGRIN ALPHA-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17 22" id="KW-0401">Integrin</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027142443" evidence="17">
        <location>
            <begin position="17"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1014"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="296"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="358"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="429"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="18">
        <location>
            <begin position="462"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="19">
        <location>
            <begin position="626"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="20">
        <location>
            <begin position="797"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013913679.1"/>
        </source>
    </evidence>
    <sequence length="1090" mass="122128" checksum="57C4B827F0925936" modified="2020-10-07" version="1">MLSLFYLCLWAGIARAFNLDTDNVVKWGGEPGSLFGFSLAMHQQLNPNKRLLLVGAPRQKAFPLQQANRTGGLFSCNIETPELPCKRIKFDETADPKKESKEDQWMGVTVQSQGPGGKIVTCAHRYEKRYYVNTEQETRDIIGRCYVLSQDLSISADEYMDGGEWSFCDGRLRGHEKFGSCQQGVAATFTRDNHYIVFGAPGTYNWKGVVRAEQKNHTLDEMGIFDDGPYEVGGESLRKESMVPVPANSYLGFSLDSGKGIISQEEVTFVSGAPRANHSGAVVLLKKDSDIQRTLSSVHIFEGEGLASSFGYDVAVVDLNHDGWQDIVVGAPQYFDRDGEIGGAVYVYINQEGKWDRVHPIRLNGSKDSMFGIAVENIGDVNKDGFSDIAVGAPYDGEFGRVYLYHGSRNGINTKPAQILDGATNNVIFFGYSITGNMDLDGNAYPDIAVGSLSDTVSVYRSRPVISIKKSIMISPEKIDLNVRNCEVSSNLCLNIRSCFEYTASESSFNQKIILDYTFDVEHERRRLGLPARVHFSNPRSVSLNGTVTLEIQQMEKCVTKRLELEEKIKDKLRPIPVSLSIAIHTPETTEKPRRQGRSLPDLTPILNASEPNTVHSEVQFLKEGCGSDNICHSNLKMHYKFCTKEGNDERYAYFPMENNVPVLVLKDQKDIALEITVTNSPSNAMHPLKDGEDAHEARLVATFPDSLTYSSLRINQAYLEKPIACRPNSNGSQVDCELGNPFKRNSTVTFYVILSTTKVNVDTKELDINLKLETTSNQTNLEPITARAKVVIELLLSLTGVAKPSQVYFGGNINGESAMKSEDEIGSLIEYEFRITNLGKPLKTLGTAFLEIMWPKELRGGKWLLYLVSIHSKELGELQCTPQNEINLLNVQRSRNSRIKREVAEKQTDGSKSFSLFSERKSKTLECNEEANCVNITCSLQGLDSKAIVVLRSRLWNSTFLEEFYKMNYLDILVRARIGLSAADNVKLTNEATNVRVTVFPAKTVALYYGVPWWIILVAILTGLLMLALLVFLLWKCGFFQRSRYDDSIPRYHAVRIRKEERQIKDGKAKENHAKKQWITKWNGNESFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDI5</accession>
    <name>A0A6I9XDI5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Death domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DTHD1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912271.1">
        <property type="nucleotide sequence ID" value="XM_014056796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDI5"/>
    <dbReference type="GeneID" id="106541372"/>
    <dbReference type="KEGG" id="tsr:106541372"/>
    <dbReference type="CTD" id="401124"/>
    <dbReference type="OrthoDB" id="6118651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28336">
        <property type="entry name" value="BA1-643"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28336:SF4">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013912271.1"/>
        </source>
    </evidence>
    <sequence length="519" mass="58588" checksum="13A80E3CBB32EAE8" modified="2020-10-07" version="1">MENVDATFEPGEMHQASDLQRFFTQANELGEPELSCSVIAPRGVLEHLTVTVVNDLSPFVVNESEELVSNVISVECSLVGQLNIAPISIAMPFNSRYRGMYKDVMVKVSDTNFQSIYLTPTSLEGHQGSQKGSAAVVRTCHLGIFSVVSCLKKETWTVPRKGLLRKLNMDPRVSFCYPPSTFSSRVTVDLKVQPIDQSTLSILKIQHDEYHSVMSTSSLVHLEYSSSLPFNRAITVVLPCPPNQDKRREGIETEAGRATSASIPRVASTHNFRGLSASPRKHRDNLKEPLKVLGYRNSEEEWILLHDVIVRNARNGLVSFDLEEPLERFIVVRLSSAMDSEHLVQFIQTLEHAIHNIMVKVVLYQNKEDPYKIIVLLVPFKELSMELQSLHKEGYCAPPEPSKPFKMREGDQVYFKFSGNIFASDDGNTYGKNYKLAFHAQRKPRLALQIKEVDEFGNYSSPHYKGTALFYKVTKDAALNNVETLPLEDHEQDSFLCKLALTLPKVHQNTETPNYFLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDY5</accession>
    <name>A0A6I9XDY5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Proteasome activator complex subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PSME4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BLM10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913839.1">
        <property type="nucleotide sequence ID" value="XM_014058364.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542585"/>
    <dbReference type="KEGG" id="tsr:106542585"/>
    <dbReference type="CTD" id="23198"/>
    <dbReference type="OrthoDB" id="17907at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990111">
        <property type="term" value="C:spermatoproteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000183">
        <property type="entry name" value="Proteasome activator complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055455">
        <property type="entry name" value="HEAT_PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23096">
        <property type="entry name" value="HEAT_PSME4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 14" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="11">
        <location>
            <begin position="216"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4-like HEAT repeat-like" evidence="12">
        <location>
            <begin position="1071"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="10">
        <location>
            <begin position="1643"/>
            <end position="1729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913839.1"/>
        </source>
    </evidence>
    <sequence length="1729" mass="198979" checksum="C41A4F6BF850FFE9" modified="2020-10-07" version="1">MMQGFARLLINLLKKKELLSRDDLELPWRPLYEMLERILYSKTEHLGLNWFPNSVESVLKTLVKNCRLYFPESATAEMLDEWRPLMCPFDVTMQKAITYFELFLPTTLPPECHHKGFKLWLDEFLGLWVSVQNLPQWEGHLVNLFARLATDNIGYINWDPYIPKIFTRILRSLNLPVGSNQMMVPRFLTNAYDIGHAVMWITAMMGGPSKLVQKHLAGLFQSIASFYHPSNNGRWLNKLMKLLQRLPSSIVRRLHRERYKKMSWLTPVPDSHKLTDQDITEFVECIIQPVLLAMFSKTGSLEAAQALQNLALMRPELVIPPVLEKTYPALETLTEPHQLTATLSCVIGVARSLVSGGKSFPEGPTHMLPLLMRALPGVDPNDFSKCMITFQFIATFSTLVPLVDCSSVLQERNDLTEVERELCSATAEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTYNTILTQCSKDIFKVALDKVFNFAVSNIFETRVAGRMVADMCRAAVKCCPEKSLKLFVPHCCNVITHLTLNDDALHDEELDKELLWNLQLLSEITRVDGKKLLPYREQLLKILQRTLHLTCKQGYILSCNLLHHLLRSTTLIYPTEYCSVPGGFDKPVSEYFPIKDWGKPGNLQKLDIQWHVPSAEEVAFAFELLDTFLQPELEKLDQYSDRNLEMSRDDVQQCLTIVHNCLIGSGNMLPPLKGEKVAHMVPSLVSLEEIKLYTGVEYDLSKENYRERICKVTRKLLHFVLDNSEDDTKSLFLVIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLQGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKVHQNLIRDLLRLSTSSYGQVRNKAQQAFFTALGTYNFCCRDIIPLVLEFLRPDGHSVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPAIVSSGLSKAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTVPETCIEVAVLMQKSVGQNGECTSLSSEEIELGIQRQKERNAESSQNYENLINKLLDCLNQRNLPWKFEHIGIGFLSLLLRDDRVLPVRAIHFFVECLNHDVLVVRKMAISAVAGILKQLKRPHKKMLICPFQISGSPQPSNIQAGDRSDNQWLHYESASLPKTKQSWEACHFVEKTHWGYYTWPQNMEIYASAEEQPKLGRSRKELSEAEQVIYDHFSDPNFVEQLIKFLSLEDRKGKDKFNPRRFCLFKGLFRNFDDTFLPVLKPHLERLAEDSHESPQRCVAEIIAGLIRGSKHWTFEKVENLWKVLCPLLRTALSNITVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLNGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDISLPNTALTKSPRVHDFTSRILEKLKPLMEVDEEIQNHVLEENGVGEEDERTQGIKLLKTILKWLMASAGRAFSTAVTEQLLLLPLFFKIAPVENDHSYDELKRDAKMCLSLMSQGLLYPQQVPLVLQVLKQIAKSNSWHARYTVLTYLQTMVFYNLFIFLNNEEAVNNIRWLVIKLLEDEQLEVREMAATTLGGLLQCHFLEIDTPMQTHFEQLCKMRLPKRRKRDLSTVVDTIPPADLVKRHAGVLGLSACILSSPYDVPTWMPQLLMDLSAHLNDPQPIEMTVKKTLSNFRRTHHDNWQQHKQQFTDDQLLVLTDLLVSPCYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEC8</accession>
    <name>A0A6I9XEC8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphatidylinositol 3-kinase catalytic subunit type 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PIK3C3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913999.1">
        <property type="nucleotide sequence ID" value="XM_014058524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEC8"/>
    <dbReference type="GeneID" id="106542713"/>
    <dbReference type="KEGG" id="tsr:106542713"/>
    <dbReference type="CTD" id="5289"/>
    <dbReference type="OrthoDB" id="9042097at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08397">
        <property type="entry name" value="C2_PI3K_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00870">
        <property type="entry name" value="PI3Ka_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000043">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="3">
        <location>
            <begin position="241"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913999.1"/>
        </source>
    </evidence>
    <sequence length="502" mass="57695" checksum="C9654B50B6934D27" modified="2020-10-07" version="1">MLRFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVAFTIWDIYGPGKAIPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKTPGKTSSTFSEDKMSRLAKLTKSHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMIEFCCVKCDDKEYAIVYYEKDGDESSPIYTSSEIVKVPDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNATTRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTHQEKALTKFLKCVNWGLPQEAKQALELLGKWKPMDVEDSLELLSSQFTNPTVRRYAVARLQQADDEDLLMYLLQLVQALKYENFDDIKSGLEPTKKETQGSVSESMAASGVTAPEIESSQIITSPLPLISSPLPTAKTKESAENENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDRDTQQRDPKTHEMYLNVMRRFSQALLKVRINFFSPIVQDGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEF3</accession>
    <name>A0A6I9XEF3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Muscarinic acetylcholine receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CHRM3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912571.1">
        <property type="nucleotide sequence ID" value="XM_014057096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEF3"/>
    <dbReference type="GeneID" id="106541595"/>
    <dbReference type="KEGG" id="tsr:106541595"/>
    <dbReference type="CTD" id="1131"/>
    <dbReference type="OrthoDB" id="10071887at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045987">
        <property type="term" value="P:positive regulation of smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046541">
        <property type="term" value="P:saliva secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000047">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000103">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001183">
        <property type="entry name" value="Musac_Ach_M3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000995">
        <property type="entry name" value="Musac_Ach_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF183">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00243">
        <property type="entry name" value="MUSCARINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00540">
        <property type="entry name" value="MUSCRINICM3R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 14 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0770">Synapse</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="94"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="329"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="386"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013912571.1"/>
        </source>
    </evidence>
    <sequence length="600" mass="67447" checksum="0170EB0793DFF817" modified="2020-10-07" version="1">MIPSSNSTIPSLLLNVSTFLNQDSQTKGLPSDDPWFTSKIWGLQSSQANESFSFPTTNPTNGSNPPDPLGGHAVWQVILIVFLTGILALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGIISMNLFTTYIIMDRWALGNLACDVWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTRRAAVMIGLAWIISFILWAPAILFWQYFVGERTVEEGKCYIQFLNQPVLTIGTAIAAFYLPVTIMCILYWRIYKETEKRTKELAGLQASGSEGEAACFVNQTGSSRSCSSYELQQQNTKHTKRRKYGGCNFWMTSKSWKPSSDQVDQEHSSSDSWNNNDGNGSLENSASSDEDDIASDTRAIYSIVLKLPGHSTILNSSKLPSSEDLPGSEDELQKLDTESKEQKPQRLHPQKSTDDENLKKCFPKLPVQQASAVYGGKASDGISTGMKASNALPLSFKEATLAKKFALKTRSQITKRKRISLIKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDCIPKTIWNLGYWLCYINSTVNPMCYALCNKTFRTTFKMLLLCQCDKRKRRRQQYQQRPSVIFQKRIPRETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEW1</accession>
    <name>A0A6I9XEW1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Rabphilin-3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RPH3A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909608.1">
        <property type="nucleotide sequence ID" value="XM_014054133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEW1"/>
    <dbReference type="GeneID" id="106539348"/>
    <dbReference type="KEGG" id="tsr:106539348"/>
    <dbReference type="CTD" id="22895"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098850">
        <property type="term" value="C:extrinsic component of synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017158">
        <property type="term" value="P:regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061669">
        <property type="term" value="P:spontaneous neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08384">
        <property type="entry name" value="C2B_Rabphilin_Doc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15762">
        <property type="entry name" value="FYVE_RP3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000032">
        <property type="entry name" value="Double c2-like domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000182">
        <property type="entry name" value="rabphilin-3A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028698">
        <property type="entry name" value="FYVE_RPH3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043566">
        <property type="entry name" value="Rabphilin/DOC2/Noc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729:SF3">
        <property type="entry name" value="RABPHILIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RabBD" evidence="11">
        <location>
            <begin position="41"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="10">
        <location>
            <begin position="89"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="453"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="159"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013909608.1"/>
        </source>
    </evidence>
    <sequence length="597" mass="65198" checksum="9F910E5B6D6DE99E" modified="2020-10-07" version="1">MTDAAVTSGPGQWGYQSDRQMALPSRAPEGHSAHGKQPERGRKGEELTDEEKEIINRVIARAEKMEEMEQDRIRRLMKRLDDMRSNMAGDGVERCVLCGEQLGPPGAACVLCEGCKKNVCTKCGIQTTNNRPHSVWLCKICTEQREVWKRSGAWFFKGLPKQTLPQPMPIAKKPQPAASRPAPSVQEPAAPYSHQRTPDRAPAAAVSAQPATYGAEPAAPTSNRGNYPPLHRKPSEARMAPSGSEYPRETRPSQDYPAEDEAARSPGGMKRASVAPAAGPRDSAAPPHQSPSAQGAGPGASPAALLQAEPPASLPAALPREESTRPGYPSVGAERPPRQGPPYGQPVVPSAAPAAPRPPPPDDDDEEEANSYDSDETTTLGALEFSLLYSQDNSSLQCAIIKAKAPPRVKNDQHICANLYLSASRVMEHMHKCPPSPFFSSSPQMDQGGEVEERGKILVSLMYNTQKGGLVVGVVRCVHLAAMDANGYSDPFVKICLKPDMGKKAKQKTQIKKKTLNPEFNEEFFYDIKHSDLAKKSLDISVWDYDIGKSNDYIGGCQLGITAKGERLKHWYECLKNKDKRIERWHALQNEHHVSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHD3</accession>
    <name>A0A6I9XHD3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prominin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PROM2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915124.1">
        <property type="nucleotide sequence ID" value="XM_014059649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHD3"/>
    <dbReference type="GeneID" id="106543598"/>
    <dbReference type="KEGG" id="tsr:106543598"/>
    <dbReference type="CTD" id="150696"/>
    <dbReference type="OrthoDB" id="6229420at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071914">
        <property type="term" value="C:prominosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF6">
        <property type="entry name" value="PROMININ-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="722"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013915124.1"/>
        </source>
    </evidence>
    <sequence length="776" mass="87932" checksum="833C108D24F463AE" modified="2020-10-07" version="1">MGPVFYKSNSEGEDDPMILRYQAGYVVCAAIAVLYFVVVPLVGLSLCCFQKNRHCGGRIKAYRRSLLCQRNLLMVSLLLTTLVLLGCVICAFAANQKVKEEMDPGARDALNTLRTLRSHLNGIPRGLRLTVEQFRVPERQILGDLNNISWSIGSTIHFLLKETVSSAMAAIKGRVQDLESSLHHLHAIHRTVQALIRDQDELASTLQDQKQSLTSLLEEPRCTYCMGALSRAQDLKLGADFQNVPSVERVLKNLYGLPQTNFSEMIQKANSSFNAIPQFTVMKMDKVVQELKKDIERVAQRVRYIEDDFPISDQTRPMKEALVKAENVSRPFLKEVKRFETYRWIAGTIACTIILLIVFCNVLGLSFGTYGLVVRDDPSDYESRAEAGAKFLITGVTFSFLFSWILILLVAGTFLMGGNIQTLVCKPWASQEIYTFIDTPGNLPPSVNVSRYLGLKQNLNLTSAYQQCKNGAGLWEVLQLKDQYSLSEHLSVAKVTSLSLHPWFPPFAKQARSLWDPSEGSQETKAHHQDQPFLLQMRNPVVGINVEGLAVDLERLSNVQSDRILAERLVEEAQELRRIQEQMVLPMETLVYTAEFQERLRAINLLFEDIILLSAGGRQDLETFRKSQVDLINYADFTAEAQFNNTESQITLVEAILPLQTKKILRQELDCFVRKELGYISQYLDWMRTSLTKDVASCQPYSTALDNGRVILCNRIMDPWNAFWFSLGGCTFFLLPGMFLALKMMKHFRPIRHKLVSTGSDETFPFHIPRVTSLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI07</accession>
    <name>A0A6I9XI07_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Paralemmin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106543815</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915374.1">
        <property type="nucleotide sequence ID" value="XM_014059899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI07"/>
    <dbReference type="GeneID" id="106543815"/>
    <dbReference type="KEGG" id="tsr:106543815"/>
    <dbReference type="OrthoDB" id="9941155at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498:SF10">
        <property type="entry name" value="PALM2 AND AKAP2 FUSION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498">
        <property type="entry name" value="PARALEMMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013915374.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="15190" checksum="9AB57177FC8AEF2E" modified="2020-10-07" version="1">MAEAELHKERLQAIAEKRKRQTEIEGKRQQLEDQILQLQHFKSKALREKWLMQGIPASSPAEEEARKRQSEEDELKVKKLEGNIHRLEQEIGKLECEESQISAKEQIILEKLKETEKSFEDLQKIFLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI19</accession>
    <name>A0A6I9XI19_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">26S proteasome AAA-ATPase subunit RPT2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Proteasome 26S subunit ATPase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PSMC1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910598.1">
        <property type="nucleotide sequence ID" value="XM_014055123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI19"/>
    <dbReference type="SMR" id="A0A6I9XI19"/>
    <dbReference type="GeneID" id="106540110"/>
    <dbReference type="KEGG" id="tsr:106540110"/>
    <dbReference type="CTD" id="5700"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000067">
        <property type="entry name" value="26S protease regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000007">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000039">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="218"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910598.1"/>
        </source>
    </evidence>
    <sequence length="440" mass="49199" checksum="ACA80683F4F86E49" modified="2020-10-07" version="1">MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDELIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM00</accession>
    <name>A0A6I9XM00_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KRR1 small subunit processome component homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KRR1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914966.1">
        <property type="nucleotide sequence ID" value="XM_014059491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM00"/>
    <dbReference type="GeneID" id="106543458"/>
    <dbReference type="KEGG" id="tsr:106543458"/>
    <dbReference type="CTD" id="11103"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="153"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914966.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="35340" checksum="7DD07856B8EF05A6" modified="2020-10-07" version="1">CRSWGVGSQLRASGVEPSQRPPCCSHFAAARNLPKTDNAAPEDESGLLTVPDGWKEPPFTREDNPKGLLEESSFATLFPKYREAYLKECWPLVQKALNEHHINAVLDLIEGSMTVSTTKKTFDPYIIIRARDLIKLIARSVPFEQSVRVLEDDIACDIIKIGSLVRNRERFIKRRQRLIGPNGSTLKALEILTSCYIMVQGNTVSALGPFNGLKEVRKVVLDTMKNIHPIYNIKAKQAEAINKRQEERNKAFIPPKEKPVVKPRKTTTETKIDIDAIKEKIKKAKKKKLGALPEEEVKLKIAAGEKKKKPSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMT4</accession>
    <name>A0A6I9XMT4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear receptor coactivator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">NCOA2</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SRC/p160 nuclear receptor coactivator family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915225.1">
        <property type="nucleotide sequence ID" value="XM_014059750.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915226.1">
        <property type="nucleotide sequence ID" value="XM_014059751.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915227.1">
        <property type="nucleotide sequence ID" value="XM_014059752.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543691"/>
    <dbReference type="KEGG" id="tsr:106543691"/>
    <dbReference type="CTD" id="10499"/>
    <dbReference type="OrthoDB" id="10035882at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016922">
        <property type="term" value="F:nuclear receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18950">
        <property type="entry name" value="bHLH-PAS_NCoA2_SRC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000007">
        <property type="entry name" value="Nuclear receptor coactivator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000008">
        <property type="entry name" value="Nuclear receptor coactivator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000008">
        <property type="entry name" value="Nuclear receptor coactivator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.20">
        <property type="entry name" value="Nuclear receptor coactivator, Ncoa-type, interlocking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056193">
        <property type="entry name" value="bHLH_NCOA1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010011">
        <property type="entry name" value="NCO_DUF1518"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032565">
        <property type="entry name" value="NCOA2/3_DUF4927"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028822">
        <property type="entry name" value="NCOA2_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009110">
        <property type="entry name" value="Nuc_rcpt_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014920">
        <property type="entry name" value="Nuc_rcpt_coact_Ncoa-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037077">
        <property type="entry name" value="Nuc_rcpt_coact_Ncoa_int_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017426">
        <property type="entry name" value="Nuclear_rcpt_coactivator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014935">
        <property type="entry name" value="SRC/p160_LXXLL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10684">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10684:SF2">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23172">
        <property type="entry name" value="bHLH_NCOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07469">
        <property type="entry name" value="DUF1518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16279">
        <property type="entry name" value="DUF4927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16665">
        <property type="entry name" value="NCOA_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08815">
        <property type="entry name" value="Nuc_rec_co-act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08832">
        <property type="entry name" value="SRC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038181">
        <property type="entry name" value="Nuclear_receptor_coactivator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01151">
        <property type="entry name" value="DUF1518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69125">
        <property type="entry name" value="Nuclear receptor coactivator interlocking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14 15 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="119"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="425"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="556"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="644"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="707"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="753"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="844"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="438"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="560"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="665"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="753"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013915225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013915227.1"/>
        </source>
    </evidence>
    <sequence length="1469" mass="160239" checksum="972805ED45DADE99" modified="2020-10-07" version="1">MSGIGENSSDPARAELRKRKECPDQLGPSPKRSTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKCAILKETVKQIRQIKEQEKAAAANEDEVQKADVSSTGQSVIDKDALGPMMLEALDGFFFVVNPEGNVVFVSENVTQYLRYNQEELMNTRVYNILHVGDHNEFVRNLLPNSRMNGGSWSGESPRRNSHTFNCRMLVKPLTDSEEESHENQEAHQKYETMQCFAVSQPKSIKEDGEDLQSCLICVARRVPMKERPLLPSSETFSTRQDLQGKITSLDTSTMRAAMKPGWEDVVRRCIQRFYAQHEGDISFAKRHHQEVLRQGLAFSPVYRFSLSDGTIVAAQTKSKLIRSQTTSEPQLVISLHMLHREQNVCGMNQDLTGQVLGKPLNTISSNSPAHQAMCSGNPGQDMTINSNMNFAINGPKEQMGMPTSRFSSSGGMNHVSSIQTSTPQGSNYALKMNSPSQNSPGMTPGQPNSMLSPRHRVSPGVAGSPRIPPNQFSPAGNLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDIKMGNLQNSPVSMNPPQLTKMGNLDSKDSFGLHGEQSEETTGQAESVCHSGEQKENNDNMSQVGSERPDGQNRLHDGKSQTKLLQLLTTKSDQMEPSSLANTMGDANKDSMGSLSGSGSAHGTSLKEKHKILHRLLQDSSSPVDLAKLTAEATGKELNQETSSTAPGSEITCKQEPVSPKKKENALLRYLLDKDDTKEIGLPDITPKLERSDSKTDPSSSTVKPVAIKTEKEEMRFEPNEQPGSELDNLEEILDDLQNSQLSQLFPDTRQVASAGSVDKQAIINDLMQITGDNSPGTPVGSQKPTGRISQSTFTNARQAQLGRMLPNQNLQLDIALQNAPGAATFPPIRNNSPYSVIPQPSMLGSQGMMGNQGNLGNNNPGMIAGNTPRPAMSSGDWGSQAAAVRVTCAATTSAMNRPIQGGMIRNPTPSLPLRSNSQAVPRQILQSQVMNMGGSELEMNMGAPQYTQQQAPPNQTAPWPDSILPIDQASFSSQNSCSSSCALCRQPFVGSPDDLMCQHPVSESPSDEGALFDQLYMALRNFDGLEEIDRALGIPELVSQQTQGVDPESFSNQEPNIILEQKAPVFTQQYASQAQMAQGNYTPLQDPNFHPMAQRPGYAALRMQPRPGLRPTGLVQNPPNQLRLQLQHRLQAQQNRQPLINQIGNVSSMNLSLRPGVPTQGNINSQMLAQRQRELLSQHLRHRQMHQVQQRTLMMRGQSMNMTPSMVASGGIPATMSNPRIPQTNPQQFPFPPNYGINQQPDPGFTGATTPQSPLMSPRMAHTQNPLIQQSQANPAYQSSTEINGWGQGNIGGNSMFSQQSPPHFGQQANTSIYNNNMSINVSMVANSAGLNNMNQMTGQISMTSATSVPTSGLPSMGPEQVNDPAMRGGSLFPNQLPGMDMIKQEGDASRKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XND8</accession>
    <name>A0A6I9XND8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106543842</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915406.1">
        <property type="nucleotide sequence ID" value="XM_014059931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XND8"/>
    <dbReference type="GeneID" id="106543842"/>
    <dbReference type="KEGG" id="tsr:106543842"/>
    <dbReference type="OrthoDB" id="6163239at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046599">
        <property type="term" value="P:regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553">
        <property type="entry name" value="ALMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553:SF22">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN ALMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="837"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1178"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="865"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="934"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="967"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="980"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915406.1"/>
        </source>
    </evidence>
    <sequence length="1260" mass="137026" checksum="869EEE21A619A9C7" modified="2020-10-07" version="1">MAQAHARTFRFRHLVPKRATPDVSAAVAERPTARLHISEAVQLAAREVDPDSSREASLSQHPLPFNLTAPNNATPGSYLSQHSLNFPEHNPAEQKHWPAYLSAKPPGPKGENSQVPCSPMWPNQPLATQRLPNENWRAAPSQEVFRLDHSLPAPLLLDLREDEMGFPKHDGFLTSGDSPCKSGLGKDPGGNVINQLTVNVDLSERAPKLMLELRQSKGQTTPPPESPRPLSDTAGKTWRRASDPETFAANLSPTLMSPRAPRLGSVDSCVIPERSLTTNRPHTGEKFQSRAEEDQRKLTRAPVDSKPRASPAFQPIPVAADISDWTDASKSNLTSVRKNELTLSGRVEREPRNNEISPFDEASFFVHLAHPIHHSTPGFCTTRALNREGPGPALPSRSGFQHPLSCLHEEMSKTLHTNARDSSATEPLQNPCPFPAKESFASHGTPAVPNDKGDGFCALQPLNGRFQSMPSLNFAEKVRAWHLSYSVEKLPEVSGLGTPVPGPGGVSPRRKDYDAIADSLTRILLKQQSLADPKAAPFYGPSSMTDLHSSQKEPPPRALPFTRSQSETSVSALSREISRTDVGNENRADDALRQADVHPDASTACRIQPTIPVEEDTLCRSYRAVPVFATVSNDEESVSTGRPSETFIEVRRMAELLRGETSSCDGRKEDLREAEEKAREPSGCGFQAGEMRMDYLRDLSPDDLNQETDSGTDSCTDFRLCSRVLSGSPPALNKLQSSLEDELQTSHHSELNIDERIPVYLHNLGIDQSPSSILTPFLPRGPIREIEFSPTELRTLKASSDVFRLHLSEDSQSVKDATQTTFNSSLLSGSALAGSAVDSDTWQPAKPSPQHTRDLLQPSSSPWKAHAVAPPLSVSVPESPPAPVSTEGTHVIPGSPSNSAGQRATRRVASPEDNLGPNQLGASRVKPGASSQDNDEKPASEEIARSFSSIPGGGRKDSFTGSDAPEESQKLPAEVDSRPSSGKFRYRLSSSSSGSLKQIDRVDLYGGPGSSERSMLEIQRGWSWDGSMAKQEITGSLNWEDLQLVDVFNKEPTVSQELRSGSQKANEDRGMTKPITRSDPEGCTRASVNQSLPLPTGRCPDIQASPSPNLQEIQSVSNMDDVAKLLRGFPCVEESRPKEIGGSQESTNSSSVDSLGIRMKKLLQYGRPAIDRAPWMETAEERDGSAPKESSVSSAGSAFLRGEGGAQRSDTGSSMDSLMVRVKTLLEEEQPVLHATQILRSIEEEEEKARGKELPGCVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNM1</accession>
    <name>A0A6I9XNM1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Pleckstrin homology domain-containing family H member 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">PLEKHH2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912323.1">
        <property type="nucleotide sequence ID" value="XM_014056848.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912324.1">
        <property type="nucleotide sequence ID" value="XM_014056849.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541404"/>
    <dbReference type="KEGG" id="tsr:106541404"/>
    <dbReference type="CTD" id="130271"/>
    <dbReference type="OrthoDB" id="6285196at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030835">
        <property type="term" value="P:negative regulation of actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13206">
        <property type="entry name" value="FERM_C-lobe_PLEKHH1_PLEKHH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17179">
        <property type="entry name" value="FERM_F1_PLEKHH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13282">
        <property type="entry name" value="PH1_PLEKHH1_PLEKHH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000021">
        <property type="entry name" value="pleckstrin homology domain-containing family H member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.530:FF:000001">
        <property type="entry name" value="Pleckstrin homology domain-containing family H member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000335">
        <property type="entry name" value="Pleckstrin homology domain-containing family H member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000125">
        <property type="entry name" value="pleckstrin homology domain-containing family H member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22903:SF3">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY H MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22903">
        <property type="entry name" value="PLEKHH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="703"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="811"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="7">
        <location>
            <begin position="955"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="6">
        <location>
            <begin position="1121"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="201"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="283"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="392"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="614"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="28"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013912323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013912324.1"/>
        </source>
    </evidence>
    <sequence length="1488" mass="168319" checksum="0A7566F7A04F7F6B" modified="2020-10-07" version="1">MADFLEPERIINWKERCMVMESQLMKFHLQASKIRELLAEKMQQLEKQVIDADHQAEKAFQQVQIMEEKLKAANVQTSESETRLYKRCQDLESLVQEKDDAIQNLEQQLVEQKEIRIQEAKIIEEKAAKIKEWVTIKLHELETENQNLRAINQNQTEEIKLLQTKLQELQGKKSVSSSQKPGEGQRLSSLTFGCFLNRARSPQQVPKPEDANSSASKQVDLNENHKSQENEIQETSLAGQIPGNNRNLKPLQQNACIPEQRMAAKSKDIDNNISKNSCITVSDWSSDEEGSKGRSKSRCVSTFSSHTSEEGTGYSRIGSEMYLTASDDSSSMLEEESFQSQGNVHKLYSWQQKSSWKNQNHLIGKSNLESFSEKKSHDSFSDELNKRFQSHRLDYSSSSSEANTPSPILTPSLAPKHPSVMLAASSQSDKQLDSPSNLPPPKLRTPSAFILSTALAKKHFSQPSLCSDKLFGKNRNAISMIRPFKPQETDIDQVDGDDTEVFEKMEISCNEESFTYDCCEMKDTETVDLSDSGKMINSKPPTPPLHRFPSWENRIYAVAKSGLCFSEVFNMDSFNKSSSSSSSYSSSGLYTSLIYKNMTTPVYTTLKGKATQISNNPFLDDSSGSEEDGSQSSSRTSESDSRSRSGPGSPRAIKRGVSLSSMTSEGDYAIPPDAYSTDTDYSEPEQKLPKTCSSSSDNGKNELLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGQIELSASSHIVRGDGKQTVQLTTEKRTYYMTADSPNILEEWIKVLQNVLKVQAASPLFIQPDVKPAVKGLLTKVKHGYSKTVWCILVGKTLYYFRSHEDKFPLGQIKLLEAKVEEVDRSCDSDEDYEASGRSLLSTHFTIVIHPKEQAPTYLLIGSKYEKDTWLYYLTIAAGSTNINVGTEFEQLVCKLLNIEGEPTSQIWRHPTLCHSKDGITSPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHVSLAQSALQVCLTHPELQNEICCQLIKQTRRRQPPNQIGPVQAWQLLALCVGLFLPQHPFLWVIKLHLKKNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLSTLNQDTGMRKPAQSGFALFTDDPSGKDIEHCLQGNIKICDIISRWEQASKEQHPGKCDGTRTVRLTYKNRLYFSVQIRGETEREKLLLMYQTNDQIINGFFPVNKDLALELAALLAQVEIGDFERPFSTPAGQVTSLSKSNQTLKQVVEKFYPKRYRDNCSEEQLRQLYQRLSTKWMALRGHTAVDCVRIYLAVVRKWPLFGAKLFAAKLLTASTPESTLIWLAVSENGINILEYNSMRLIMTYLYKNLVTFGGYQEDFMLVINNISTEEKHTEKLLFTFAKPKILEITLLIASYINNFHQQKKTVHEFSAPQSRNPKSNDIECHSFLLQNRPTKGPTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQC0</accession>
    <name>A0A6I9XQC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="43">Diacylglycerol kinase</fullName>
            <shortName evidence="43">DAG kinase</shortName>
            <ecNumber evidence="43">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="47" type="primary">DGKZ</name>
    </gene>
    <organism evidence="46 47">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="47" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1,2-di-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycerol + ATP = 1,2-di-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40351"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77125"/>
            <dbReference type="ChEBI" id="CHEBI:77126"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:40352"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1,2-didecanoyl-sn-glycerol + ATP = 1,2-didecanoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43428"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18155"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:78227"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:43429"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1,2-ditetradecanoyl-sn-glycerol + ATP = 1,2-ditetradecanoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:80651"/>
            <dbReference type="ChEBI" id="CHEBI:83550"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:43445"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycerol + ATP = 1-(9Z)-octadecenoyl-2-hexadecanoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43420"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:74551"/>
            <dbReference type="ChEBI" id="CHEBI:75447"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:43421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1-O-alkyl-2-acyl-sn-glycerol + ATP = 1-O-alkyl-2-acyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44072"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52595"/>
            <dbReference type="ChEBI" id="CHEBI:73332"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:44073"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40403"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77184"/>
            <dbReference type="ChEBI" id="CHEBI:77186"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:40404"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-O-hexadecyl-2-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-O-hexadecyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40407"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77185"/>
            <dbReference type="ChEBI" id="CHEBI:77187"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:40408"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1-O-hexadecyl-2-acetyl-sn-glycerol + ATP = 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41676"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:75936"/>
            <dbReference type="ChEBI" id="CHEBI:78385"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:41677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-O-hexadecyl-sn-glycerol + ATP = 1-O-hexadecyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41672"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:34115"/>
            <dbReference type="ChEBI" id="CHEBI:77580"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:41673"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>1-eicosanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycerol + ATP = 1-eicosanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77094"/>
            <dbReference type="ChEBI" id="CHEBI:87223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:40332"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40335"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:72864"/>
            <dbReference type="ChEBI" id="CHEBI:77096"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:40336"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:64839"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:43417"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40359"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77129"/>
            <dbReference type="ChEBI" id="CHEBI:77130"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:40360"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40323"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:40324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43424"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:74560"/>
            <dbReference type="ChEBI" id="CHEBI:75468"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:43425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>a 1-O-alkyl-sn-glycerol + ATP = a 1-O-alkyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15850"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58014"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.93"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:16938"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="41">Glycerolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 43">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="43"/>
    <dbReference type="RefSeq" id="XP_013918239.1">
        <property type="nucleotide sequence ID" value="XM_014062764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQC0"/>
    <dbReference type="GeneID" id="106546003"/>
    <dbReference type="KEGG" id="tsr:106546003"/>
    <dbReference type="CTD" id="8525"/>
    <dbReference type="OrthoDB" id="242257at2759"/>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047649">
        <property type="term" value="F:alkylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20849">
        <property type="entry name" value="C1_DGKzeta_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20895">
        <property type="entry name" value="C1_DGKzeta_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000034">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047485">
        <property type="entry name" value="C1_DGKzeta_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047484">
        <property type="entry name" value="C1_DGKzeta_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056383">
        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF43">
        <property type="entry name" value="DIACYLGLYCEROL KINASE ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 42" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="17 43" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 43 47" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 43" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="46" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 43" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DAGKc" evidence="45">
        <location>
            <begin position="525"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="42">
        <location>
            <begin position="1084"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="42">
        <location>
            <begin position="1120"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="44">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="44">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="44">
        <location>
            <begin position="485"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="44">
        <location>
            <begin position="1014"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="44">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="44">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="44">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    <evidence type="ECO:0000256" key="18">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
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            <dbReference type="ARBA" id="ARBA00023395"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
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            <dbReference type="ARBA" id="ARBA00023400"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
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            <dbReference type="ARBA" id="ARBA00023411"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034614"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
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            <dbReference type="ARBA" id="ARBA00034638"/>
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            <dbReference type="ARBA" id="ARBA00034642"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034647"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00049500"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00050429"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050690"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="37">
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00051725"/>
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    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00060536"/>
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    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
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    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="45">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
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    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="47">
        <source>
            <dbReference type="RefSeq" id="XP_013918239.1"/>
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    </evidence>
    <sequence length="1191" mass="134236" checksum="02DCF941A2FB76F9" modified="2020-10-07" version="1">MESFFKKHFRRKIASSGPQRTSVVALPASRARRRSVVGQPSTILVERRRRSSVQLQEMSCLGSSRKRRLCSRRRSSTTTPCMSPRFSVQKKRGGHVHTIDTHLIGPSLLLASLIQVYKEEERRQSHCTESSSSGSPNDGMVDSDWSEEALSSGSEGAEFEKKELQTECSLYPIPPIQHVPDLRPFLRDPYYLRRRSSHLLPAEFVHSRAAYGLQGRYRYFNQRRCSGETPKPSALLQPCSFERAARRWKVPVGTCWLPLSTHKCLGVDPALSSCGSDSSEGYNALLTKAIAKSGLQHMVAQPTPTFALRSDSEREIRNSVDWSETAVYGEHIWFETNVSGDFCYVGEQNCVSKILQKPMSKRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNIREPTVVRHHWVHRRRQEGKCRQCGKGFQQKFAFHSKEIVAISCSWCKQAYHSKVSCFLLQHIEEPCSLGAHAAVVIPPTWILRVRHPQNPLKSSKKKKRTSFKRKSSKKGPEEGRWKPFVIKPIPAPLMKPLLVFVNPKSGGNQGTKIFQSFMWYLNPRQVFDLSQGGPKEALELYRKVHNLRILACGGDGTVGWILSILDQLRLNPPPPVAILPLGTGNDLARTLNWGGGYTDEPLSKILSHVEEGEIVQLDRWNLQVDPTPEGNPEEKDETATDKLPLDVFNNYFSLGFDARVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKLVCDGTDLTPKIHDLKPQCLVFLNIPRYCAGTMPWGNPGEHHDFEPQRHDDGCLEVIAFTMTSLAALQVGGHGERLYQCREVVLTTSKAIPMQVDGEPCKLGASCIRISLRNQANMVQKTKRRNSMPLLNDQQPVPERLRIRVSRIGMHDYEALHYDKEKLKEASVPLGIIVVPGDSDLEFCRTHIERLQEDFPSCTSTIQNLFPQEGDGAKPKILSCQKLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVRTQTVGTSAMPDLVDVPSTPTKQHLPLPISPPSTPVSSSAVDSSQTLKDDALIDAAKNNDFSKFKELHRAGKDLMMRDPTGQTVLHHAVKSGSKEIVKYIIENAPAEILDVAEEENGETSLHQAAALRQRTICHYIVEAGASLMKTDVQGDTPKHRAEKANDPDLAAYLENRQHYQMIQREDQETAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR25</accession>
    <name>A0A6I9XR25_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 15">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PGK1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 15">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 15"/>
    <dbReference type="RefSeq" id="XP_013916221.1">
        <property type="nucleotide sequence ID" value="XM_014060746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR25"/>
    <dbReference type="GeneID" id="106544469"/>
    <dbReference type="KEGG" id="tsr:106544469"/>
    <dbReference type="CTD" id="5230"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000019">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000031">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="345"/>
            <end position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013916221.1"/>
        </source>
    </evidence>
    <sequence length="389" mass="41453" checksum="1652849C56624FC4" modified="2020-10-07" version="1">MKNNQITNNQRIKAAIPSIKHCLDNGAKSVVLMSHLGRPDGVPMPDKFSLEPVVAELKSLLGRDIIFLKDCVGSEVESACASPADGSVILLENLRFHVEEEGKGKDLSGNKIKADSAKVDAFRASLSKLGDVYINDAFGTAHRAHSSMVGVNLSQKAAGFLMKKELDYFAKALESPEHPFLAILGGAKVQDKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGNSLFDEEGSKIVKDLMAKAEKNGVRITLPVDFITADKFDENANTGVATLASGIPAGWMGLDCGPESTKLFVEAVGRAKQIVWNGPVGVFEWEKFAKGTKAVMDKVVEVTSKGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT32</accession>
    <name>A0A6I9XT32_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">G-protein coupled receptor 126</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ADGRG6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913828.1">
        <property type="nucleotide sequence ID" value="XM_014058353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT32"/>
    <dbReference type="GeneID" id="106542576"/>
    <dbReference type="KEGG" id="tsr:106542576"/>
    <dbReference type="CTD" id="57211"/>
    <dbReference type="OrthoDB" id="10037534at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000050">
        <property type="entry name" value="Adhesion G protein-coupled receptor G6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057333">
        <property type="entry name" value="SEA_Gpr126"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF41">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25307">
        <property type="entry name" value="SEA_Gpr126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026834903" evidence="4">
        <location>
            <begin position="40"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="45"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="6">
        <location>
            <begin position="158"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913828.1"/>
        </source>
    </evidence>
    <sequence length="531" mass="59883" checksum="69C1293D5BDA972B" modified="2020-10-07" version="1">MLTETKMLYEIQLWCCQWGGKSHLFFLFILSLLCIQISAQECSECRMVLSNPTGIFTSPCFPNNYPNNQACKWTIRAPSGYIIQTTFVDFDIEEASGCKYDHVSLDTGDKKDSYCGMTAKGLSFNSSRNEMIVSFTSDFSIQKKGFSATYTRIAVSLKNQKVVIPQFLDSESVSLANSIHVPELNQFTLCFEARENNSNNYEWKAFSYGDSSVEFFSFGKTEKGHFIFISDSQCFLNDALDIGPGDNIFTETFEELCLVWDSSSGIVGINIKETYKTVHCLDTYGKVIPGDGKLVLGSNREDINPLPGDMYNFRLWNFTMNSQSLFNLTCDDKGNIIDWENDFWSIPTTFLKAENNLSCGSYLVLLPTVEPTSCATTGSLCRATVSSTTPSPTVTTNMPDTNRTDKIMDGRLYYRIDFTVYRIKEDAEPNIQNVIREWLIQTMGTWNYNVCVVDVSVEPSSTKNAARGKRSVNQSFEVRALLAFNSTNNVSLEEQTIRKKLENSNITENLKLHNVIVHSIGKDRGFFCCCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTL8</accession>
    <name>A0A6I9XTL8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">INO80 complex subunit E isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">INO80E</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013917372.1">
        <property type="nucleotide sequence ID" value="XM_014061897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTL8"/>
    <dbReference type="GeneID" id="106545372"/>
    <dbReference type="KEGG" id="tsr:106545372"/>
    <dbReference type="CTD" id="283899"/>
    <dbReference type="OrthoDB" id="5977486at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026678">
        <property type="entry name" value="INO80E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056515">
        <property type="entry name" value="INO80E_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21812">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21812:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24237">
        <property type="entry name" value="INO80E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit E N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013917372.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="26424" checksum="9C003746C8BF3DF4" modified="2020-10-07" version="1">MNGAGEPDPGGGGYKRRYRALKRRLKFLIYEQECFQEELRKAQRKLLKVSRDKSFLLDRLLQYENVDEDSSDSEATASSDNSDGEMPKGTEPQSLKRKHSPQLGSASSPSSSGLSLQPTSGFGLPASGTSSPYLASLNSPTYAPFPSDYLALGPEPVPPQPDSNATRSARRPARPHTLKLPLPPASHSDCSSVVVGLPFPSPRGTPKLPPPTILSTVPHQMFSDAGEGSGEDPLDGDDELVIDIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XU11</accession>
    <name>A0A6I9XU11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106547072</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919584.1">
        <property type="nucleotide sequence ID" value="XM_014064109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XU11"/>
    <dbReference type="GeneID" id="106547072"/>
    <dbReference type="KEGG" id="tsr:106547072"/>
    <dbReference type="OrthoDB" id="6151005at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="39"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013919584.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="27010" checksum="2445755D8FE27062" modified="2020-10-07" version="1">MDNQTTVPYFLLLEFSKSWHLQILHSFLFFVLYLSTITANLLIISAVAIDNRLHSPMYWFLMNLAVQDLGSVSVIVPKSIVNSLMDTRHISYLGCVAQVFLFVSFAASDFSLLTVMAYDRYVAICNPLHYEMVMNWKACTEIIILVWIISLLYGTLHTTGTFSNLFCSNVVNQFFCEIPHLLKLSCSGFNLIEIGVIVNLNLQLKNITTVSRSFRGCCVHSLVHSLKQSLQSNATNYTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUI1</accession>
    <name>A0A6I9XUI1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Zinc finger protein 318-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC106542975</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914335.1">
        <property type="nucleotide sequence ID" value="XM_014058860.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914343.1">
        <property type="nucleotide sequence ID" value="XM_014058868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUI1"/>
    <dbReference type="GeneID" id="106542975"/>
    <dbReference type="KEGG" id="tsr:106542975"/>
    <dbReference type="OrthoDB" id="9909793at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055309">
        <property type="entry name" value="Znf318-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15577">
        <property type="entry name" value="ZINC FINGER CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15577:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="653"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914343.1"/>
        </source>
    </evidence>
    <sequence length="728" mass="83537" checksum="5F77DD6D9B13B567" modified="2020-10-07" version="1">MNRDDLADGPIFSRVLSHPWNLERGFFREENPSRPFSMRHDEDSYNRDIFIAQLDRSINSEFLQHQSRENKRGGEQESKCFQYGRDDRLFDESIKSRALLAETEKYCKQGLNRSPSLIYMDEDFRKLEKIRRKREEDLRSRNISTDYPMPDSANQEQSSESRHHYRSEKTPAMSLQSILKKRSDDSSIQDFRDFSKGKKPPESTSEPVTQRSDFLLPNERASQDGSGFSCILGTTTDSVYAPEKILDPFPYNIEDEEKFLYGDDDGDSNNCVYSQKKMMSKKKKPVREKVKYLPIAKPKNLKQSVKLKNLEQSVKQKNLEQSVKPKNLEQSVKPENLEQSVKPENLEQSVKPENLEQSSVEYDKMRDLLKTIGLDIGMAEIDKLAARTEERLHGKKTAYALNHHSVANHKAKLEEKQKHSLSPFDSFPSPEALSPVLKYDYNNIAISGQNKATDMCEQSSVPGSVIPSAPPSFLDIPPGSISGSCHDVPYVSTFTPTQFFPNSASLPVALPDYDVYQHHMDYATSDWCPQQVDSLCPSKTDVADLKDRCINRNLRIIDTINISKSKQFQKAATTTPYSNQLSKSSKKRAKRRNAAARKIALLNHKVTEECIKSELEQESYHKKLFYHKIELDRLSKQQAEMLQKKRKEKDPLLTELSRSKENLAKKVAQLELTIKILKEKQSELDKITHSVEMNHFGKSQKLSSENKDSSENNLQKNPQSEEITFNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV79</accession>
    <name>A0A6I9XV79_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GTPase-activating protein 30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ARHGAP30</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920034.1">
        <property type="nucleotide sequence ID" value="XM_014064559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV79"/>
    <dbReference type="GeneID" id="106547404"/>
    <dbReference type="KEGG" id="tsr:106547404"/>
    <dbReference type="CTD" id="257106"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04384">
        <property type="entry name" value="RhoGAP_CdGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000002">
        <property type="entry name" value="rho GTPase-activating protein 32 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051576">
        <property type="entry name" value="PX-Rho_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15729">
        <property type="entry name" value="CDC42 GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15729:SF12">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="24"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013920034.1"/>
        </source>
    </evidence>
    <sequence length="421" mass="47095" checksum="FCA7319B739E034F" modified="2020-10-07" version="1">MSLAMKARQKVKRKGPAKDRVFGCDLVEHLQSSGRDIPHVLKSCTEFVEQHGIIDGIYRLSGGSSNTQKLRMEFDNDRNPEINKEVYLKDIHCVSSLCKAYFRELPNPLLTYQLYDKFAEAVAIQLEESRLEKIKEVLKELPSPHHRTLEFLMKHLVHMASFSSQTNMHARNLAIVWAPNLLRSKDIEASGFNGTAAFMEVRIQSIVVEFILTHVEQIFGNAPLCSGTREAIRRSLLALSPGVLPEYCTPYNVPSMLNQGDGPPQMRPYHTIIDLDESKRKGSVKAKKWRSFFNLGRSSHDSKRKLNKAEDKDYKLGKMRLRPAKSMDSLSCMASVNDNDARLGAEIPPKHECFDGPVSLDSSFSESAYMSAKSKCEDTQEEATKSEPTTPKLSKSGLIAGTSQGRSPKSSSNRAEKCAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVB8</accession>
    <name>A0A6I9XVB8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106545628</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917741.1">
        <property type="nucleotide sequence ID" value="XM_014062266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVB8"/>
    <dbReference type="GeneID" id="106545628"/>
    <dbReference type="KEGG" id="tsr:106545628"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015193">
        <property type="term" value="F:L-proline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005298">
        <property type="term" value="F:proline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015816">
        <property type="term" value="P:glycine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904271">
        <property type="term" value="P:L-proline import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002438">
        <property type="entry name" value="Neutral_aa_SLC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF44">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT TRANSPORTER XTRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01206">
        <property type="entry name" value="ORPHTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 9" id="KW-0769">Symport</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013917741.1"/>
        </source>
    </evidence>
    <sequence length="406" mass="46148" checksum="628F690C930F1573" modified="2020-10-07" version="1">MDKSRPLWDNHFQFIFACISYAVGLGNVWRFPYLCQMHGGGGFLIPYLIMLIVEGVPLLFLELAVGQIMHQGSIGTWNAISPYLCGVGIASVVVSFFLSMYYNVINGWAFWYLFHSFQDPLPWASCPLNINHTNYEEECEKATPTQYFWYRKTLNISPSIEESGSIQWEQAACLILAWLVVYLCILRGTEYTGKVVYVTAILPYIVLIIYLIRVLTLHGATNGLRYMFTPKLEQLANPKTWINAATQIFFSLGLGFGSLIAFASYNEPNNNCERHAIIISLINSATSIFASIVTFSIYGFKATFNYESCINKVILLLTNVFDLKEDLLTTENLENMKQYLASVHPGKYAEVLPQIKNCSLEIELDTVSLPSFFSRKNMMKSFVDVKKFGQYLEYEIADLLRMGDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVF9</accession>
    <name>A0A6I9XVF9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Prolactin regulatory element-binding protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PREB</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918077.1">
        <property type="nucleotide sequence ID" value="XM_014062602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVF9"/>
    <dbReference type="GeneID" id="106545892"/>
    <dbReference type="KEGG" id="tsr:106545892"/>
    <dbReference type="CTD" id="10113"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013918077.1"/>
        </source>
    </evidence>
    <sequence length="439" mass="48001" checksum="E1FFBBB9B6397ACF" modified="2020-10-07" version="1">MPPRRPPELYRAPFPLYALRIHREAGLALIAGGGGAAKTGITNGLHFLQLDWRDGKLSASLLHAHDTETRATMTMALAGNFIAAGQDATCHILRFQLFKSGQKTGGSSGDSAKTGAGDKDLRRRKAPVPNEKSNGNTHREMTEVAVETLHCVQTDTSPDTLQKAVCFSDDHTLMATGGVDGFLRVWEFPSMKKTLEFQAHNGEIEDIALGPDNKVVTVGRDFQCCVWQRDQMVTGLRWNENLPGIPDKTYRYQSCKFGAVEDQAKALRLYTVQIPYKRERRPLPCYITKWDGHSFLPLLTRPCGNEVISCLSVSETGTFLGLGTVTGSVAIFVAFSLQRLYYVREAHGIVVTAVAFLPETPELLKSNEAALLSVAVDSRCRLHQIACRRMFKAGVVDLNVGWESCPANMGSPTNSWLIQCSFSSQGVSPSGCCCSSVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVJ7</accession>
    <name>A0A6I9XVJ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear pore complex protein Nup50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NUP50</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914673.1">
        <property type="nucleotide sequence ID" value="XM_014059198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVJ7"/>
    <dbReference type="GeneID" id="106543237"/>
    <dbReference type="KEGG" id="tsr:106543237"/>
    <dbReference type="CTD" id="10762"/>
    <dbReference type="OrthoDB" id="10062131at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914673.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="30003" checksum="E395DD63F48337C5" modified="2020-10-07" version="1">MIQRLHLCDPWKEGSVVSSVSANNITVEKTSAHAEANGENPEPLSPGLTQSKACNSKIYHKQLAALNCSVRDWIVKHVNTNPLCDLTPIFHDYERYLADIEQKGLAGSGSSGSSSKATEAQPRPAFGSTALPQSSTFLFSSKSEETLGKKTELEKKTELKPAAASTTTLFSFGKSGKSGLSSGAAGSGFSFSPGNGGLFGKDASQTKVASSFSNNALDAPAEKAGNTQTGGDEEEEEPPKVVINEVKEEDAFYSKKCKVFYKKDNEFKEKGVGTLHLKPASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWN8</accession>
    <name>A0A6I9XWN8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Sphingosine 1-phosphate receptor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">S1PR3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915048.1">
        <property type="nucleotide sequence ID" value="XM_014059573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWN8"/>
    <dbReference type="GeneID" id="106543533"/>
    <dbReference type="KEGG" id="tsr:106543533"/>
    <dbReference type="CTD" id="1903"/>
    <dbReference type="OrthoDB" id="9874984at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038036">
        <property type="term" value="F:sphingosine-1-phosphate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15345">
        <property type="entry name" value="7tmA_S1PR3_Edg3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000098">
        <property type="entry name" value="Sphingosine 1-phosphate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004062">
        <property type="entry name" value="EDG3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004061">
        <property type="entry name" value="S1P_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01524">
        <property type="entry name" value="EDG3RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01523">
        <property type="entry name" value="S1PRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="57"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="179"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="270"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604061-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013915048.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="42252" checksum="E21A8B4DABA1B164" modified="2020-10-07" version="1">MAIVSTAPPSFLFFPENEVVDPVIVLHYNYTGKLSRGKEKKLTIIALLIICGFIILENLMVLIAIWKNNRFHNRMYFFIGNLALCDLLAGIVYKVNILMSGNKTLTLSSTIWFVREGSMFVALGASTLSLLAIAIERHLTMIKMRPYDANKKYRVFLLIGTCWLISLSLGALPILGWNCIGNLRDCSTILPLYSKKYVAFCTIIFIAILVAIVILYARIYILVKSSSRNVTNHNNSERSMALLRTVVIVVGVFIACWSPLFILLLIDVACKVKECSVLYQEDGFIALAVLNSAMNPVIYTLASKDMRRAFFRLVCGCLVKNNVAMSLPIQPTPDQSRSKSSSSNNTPKLNDDFLKTKNLSYITEKNKPPFYNGNVCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX86</accession>
    <name>A0A6I9XX86_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Prostaglandin F2 receptor negative regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PTGFRN</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918416.1">
        <property type="nucleotide sequence ID" value="XM_014062941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XX86"/>
    <dbReference type="GeneID" id="106546158"/>
    <dbReference type="KEGG" id="tsr:106546158"/>
    <dbReference type="CTD" id="5738"/>
    <dbReference type="OrthoDB" id="9873136at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000191">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001070">
        <property type="entry name" value="Prostaglandin F2 receptor inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002026">
        <property type="entry name" value="Prostaglandin F2 receptor inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000854">
        <property type="entry name" value="Prostaglandin F2 receptor negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF3">
        <property type="entry name" value="PROSTAGLANDIN F2 RECEPTOR NEGATIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026896344" evidence="10">
        <location>
            <begin position="24"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="839"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="127"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="283"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="414"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="696"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013918416.1"/>
        </source>
    </evidence>
    <sequence length="887" mass="100774" checksum="D799AC5F3A840EFE" modified="2020-10-07" version="1">MGHRAKKAPLSLLLLVLAVSCHGRIVKVPEGPLIRVEGTEVVIPCNVSQYGGPSEQNFDWEFSANLGQDSKFVHILSTWDPSFMSPEYQDRVNRGEIMLRRKSNSTVEFVIGDIHPTDQGIYRCTTPSTDSVVQGNYNDVVEVKVIADGLTVTGSNFYSSSTWNVSEGDTFQLRCSASTNSTEHTHLQVSWEFKGNHVWQEILSLTHEGKFRIGSEYSERYISGDVRLDAMANDIYSLSLSQALPTDEGAYRCRVSEWVKGADGSWQKIQEKTADIVNLVVKPTSLDVFITRSNISVLERESLELTCNITTDRSGIFQTEITWYFNESPDGTMAEAQVLLNADRDLVISDSTFISPSHVDRSNYNLLMRDVGTENSGYYYCQAALWVPQHNGSWHKVAEKISVPVTVEVVTLEPNYYVFLNASKTPLFAEDPTELECRIMEVQNTETNVHFTVSWYYRMPTRSDEVVEYELLATMDADWTLVLGEKSKQRAQNGEIIFSKPKVDTFRLRIQWTSETDRGEYYCVISSWSRQRNNSWIKIKDVASMPISILWSTQDYTLTVEAVKLKPFFMAGHTFEMTCKVSSQNIKTPRYSVLITAMKSLSDRTRTNGTTRIISLNQDSVVRREDWTDQDRVDGVVLEKIQENEFRYRMYQTQISDAGLYRCEVTAWSPGGGGRWRKAVDGFSNPIQIDFQTSGPVFNVSVYSDSPTIYRGELVNLLCIITIETTVLDPDDMSFDVSWFATRSFAMDKEPVFLASLDRKGIVTQARRNGSSDVSLERISPMEYRLRVHGCEDHDFGNHFCLVNPWVRSAAGVWQQEKEIKSKAILVTVKMDVLNAFKIPLLIGIGLSSVIGLLSCLIGYCSSRWCCKKEVQETRRERRRLMSMEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY53</accession>
    <name>A0A6I9XY53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Transmembrane protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">TMEM9</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909400.1">
        <property type="nucleotide sequence ID" value="XM_014053925.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909401.1">
        <property type="nucleotide sequence ID" value="XM_014053926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY53"/>
    <dbReference type="GeneID" id="106539194"/>
    <dbReference type="KEGG" id="tsr:106539194"/>
    <dbReference type="CTD" id="252839"/>
    <dbReference type="OrthoDB" id="10059035at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008853">
        <property type="entry name" value="TMEM9/TMEM9B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13064:SF1">
        <property type="entry name" value="PROTON-TRANSPORTING V-TYPE ATPASE COMPLEX ASSEMBLY REGULATOR TMEM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13064">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05434">
        <property type="entry name" value="Tmemb_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636616" evidence="7">
        <location>
            <begin position="21"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013909400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013909401.1"/>
        </source>
    </evidence>
    <sequence length="182" mass="20937" checksum="BF3F4DE02CA0A9D2" modified="2020-10-07" version="1">MNSVSLAAIFWCILIPSFETSKTFEDIRCKCVCPPYRNITGQIYKNNVSQKDCNCLHVVDPMPVPGHDVEAYCLLCECKYEERSTTTIKIIIIIYLSVIGALLLYMVFLMVVDPFIRKPDAYTQPLHNEEESEDPCSLTAIRPSASARTNTVLERVEGAQERWKRQVQEQRKTVFDRHKMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY93</accession>
    <name>A0A6I9XY93_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="25">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">ABL2</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="25"/>
    <dbReference type="RefSeq" id="XP_013918761.1">
        <property type="nucleotide sequence ID" value="XM_014063286.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546416"/>
    <dbReference type="KEGG" id="tsr:106546416"/>
    <dbReference type="CTD" id="27"/>
    <dbReference type="OrthoDB" id="98077at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000145">
        <property type="term" value="P:regulation of cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09935">
        <property type="entry name" value="SH2_ABL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11850">
        <property type="entry name" value="SH3_Abl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.330:FF:000003">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000010">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000037">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000004">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035837">
        <property type="entry name" value="ABL_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015015">
        <property type="entry name" value="F-actin-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08919">
        <property type="entry name" value="F_actin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00808">
        <property type="entry name" value="FABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 24 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 25 31" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0519">Myristate</keyword>
    <keyword evidence="9 24 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16 22" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3 23" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="17 25" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SH3" evidence="28">
        <location>
            <begin position="88"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="27">
        <location>
            <begin position="154"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="269"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="526"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="678"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="874"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="741"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="26">
        <location>
            <begin position="892"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="31">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013918761.1"/>
        </source>
    </evidence>
    <sequence length="1094" mass="119768" checksum="1506AC4A3A33B6C5" modified="2020-10-07" version="1">QQQQQLLPLAQQPQSQPRGLRGSNSSGSGRPASRRRETAAARAADSGFNIFTQHEALHRPFGCDAEPQTLNEAIRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNQNGEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRESESSPGQLSISLRYEGRVYHYRINTTTDGKVYVTAESRFNTLAELVHHHSTVADGLVTTLHYPAPKCNKPTVYGVSPIHDKWEMERTDITMKHKLGGGQYGEVYVGVWKKYNLTVAVKTLKEDTMEVEEFLKEASVMKEIKHPNLVQLLGVCTLEPPFYIVTEYMPYGNLLDYLRECNREEVTAVVLLYMATQISSAMEYLEKKNFIHRTILPVNTQLLGCIENILTRTAGIAKSALDQXTVLMQFLTLKVAFLVAIISARRILELQALSERTCTQSNQRTDSVNPYMNAIPTFLEKVYQLNISISLVNSKAVSSPFSVKEAQSYCITPEQQTFLFFCLGFIRSTQQTSGSPALPRKQRDKSPSGLLEDTRETTFTRDRKGGFFSSFMKKKNAPTPPKRSSSFREMENQAHKKYELTGNFSGVGPLQHIDGFSFTPSQQDGNLCLSKCYIGSFAQRNFYGEDGGGGGGSGSSGGTSIGSGWSGLTGFFTPRLIKKTFGLRAGRPTGGDEASKPFPRSNSTSSMSAGLPEQERMAMTLPRNCHRSKVQLERTVSTSSEPEENTDKPNDLLPKRSEEIHTLTRSRPKTKLLPRGATAVPFRTPSVDTSVSEKDGQGPTATASLKSKEKNSGMRQGSVEGEDKPGWPSPAKAAAILPTTHNHKVPVLISPTLKHTPAEVQLIGTDSQGNRFKLLSEHQGAASSDRDRPRRIKPKCAPPPPPPPPPQAVLRLLHQPPGTSDTVEELDNGALVQPGQESEGSKKVTAAPTGGKAGRPIMPPPQVPSPLSSSPAKMANGTAGAKVALRKTKQTAERISADKISKEALLECADLLSSAIAEPTTNSQLVDTGHQLLDYCSGYVDCIPQTRNKFAFREAVSKLELSLQELQVSSMAANLPGTNPVLNNLLSCVQEISDVVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYG4</accession>
    <name>A0A6I9XYG4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium leak channel non-selective protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106548434</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921274.1">
        <property type="nucleotide sequence ID" value="XM_014065799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYG4"/>
    <dbReference type="GeneID" id="106548434"/>
    <dbReference type="KEGG" id="tsr:106548434"/>
    <dbReference type="OrthoDB" id="10069766at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032224">
        <property type="term" value="P:positive regulation of synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032230">
        <property type="term" value="P:positive regulation of synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000065">
        <property type="entry name" value="sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000066">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000030">
        <property type="entry name" value="sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000038">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028823">
        <property type="entry name" value="NALCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46141:SF1">
        <property type="entry name" value="SODIUM LEAK CHANNEL NALCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46141">
        <property type="entry name" value="SODIUM LEAK CHANNEL NON-SELECTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="575"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="36"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="384"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921274.1"/>
        </source>
    </evidence>
    <sequence length="687" mass="79264" checksum="1A600AB44DC41343" modified="2020-10-07" version="1">MLKRKQSSRVEAQPVTDFGPDESLSDNADILWVNKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEISDIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGPFTYHCVTNDTKPGNITWNNLAIPDTHCSKASEEGYQCPPGFECLDLEELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDATGGWQLVDVDVNKPQGRAPACLQRMMRSSIFHMFILSMVAVDVIVAASNYYKGETFKSQYDEFYLAEVAFTVLFDLEALLKIWGLGFTGYISSSLHKFELLLVIGTTLHIYPVLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTTCLLRRLPSASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYP1</accession>
    <name>A0A6I9XYP1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PRMT1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48104"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:48105"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013918951.1">
        <property type="nucleotide sequence ID" value="XM_014063476.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546578"/>
    <dbReference type="KEGG" id="tsr:106546578"/>
    <dbReference type="CTD" id="3276"/>
    <dbReference type="OrthoDB" id="9007053at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF54">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="13">
        <location>
            <begin position="72"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="14">
        <location>
            <begin position="155"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013918951.1"/>
        </source>
    </evidence>
    <sequence length="221" mass="25730" checksum="4684D126590668A6" modified="2020-10-07" version="1">MTWAFMYFHQVSCTQLENSSKPTAEEMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGAKRVIGIECSSISDYAVKIVKANKLDHVVSIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVIYARDKWLEVDIYTVKVEDLTFTSPFCLQVKRNDYIHALVAYFNIEFTRCHXNIYQIPFCPKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZR6</accession>
    <name>A0A6I9XZR6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Testis development-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106539684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013910005.1">
        <property type="nucleotide sequence ID" value="XM_014054530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZR6"/>
    <dbReference type="GeneID" id="106539684"/>
    <dbReference type="KEGG" id="tsr:106539684"/>
    <dbReference type="OrthoDB" id="9940890at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031399">
        <property type="entry name" value="TDRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35663:SF3">
        <property type="entry name" value="GENE, 30191-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35663">
        <property type="entry name" value="TESTIS DEVELOPMENT-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15683">
        <property type="entry name" value="TDRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910005.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="20712" checksum="78C0D9A254028286" modified="2020-10-07" version="1">MTQGFWRAYKTKVMQALGGELQEEDLQDESENPQLVETTDSIMLSEEGLNSVSQLARRVQGAGAKGWRTMSSLFSREDEDQLLAPEAAADHPLAAKPAEEEAEKKAAGLWDVFATKWQQTSALEKLTAKVDSSEEKVEGSGGSGVMPAEEVNDLGHDNDLREAEGVAFKWSFVTNKLAELKNKNIPKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y098</accession>
    <name>A0A6I9Y098_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cilia- and flagella-associated protein 57</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">WD repeat-containing protein 65</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CFAP57</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Associates with components of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility, and might act as an inner dynein arm (IDA) hub or linkage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">May form homodimers. Associates with components of the nexin-dynein regulatory complex (N-DRC) and the CFAP184:CFAP263 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CFAP57 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919696.1">
        <property type="nucleotide sequence ID" value="XM_014064221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y098"/>
    <dbReference type="GeneID" id="106547156"/>
    <dbReference type="KEGG" id="tsr:106547156"/>
    <dbReference type="CTD" id="149465"/>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000014">
        <property type="entry name" value="AGAP008095-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000357">
        <property type="entry name" value="Cilia and flagella associated protein 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000271">
        <property type="entry name" value="cilia- and flagella-associated protein 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0969">Cilium</keyword>
    <keyword evidence="5 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="18" id="KW-0282">Flagellum</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="385"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="16">
        <location>
            <begin position="392"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="508"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="634"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1170"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="694"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="917"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1098"/>
            <end position="1164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013919696.1"/>
        </source>
    </evidence>
    <sequence length="1249" mass="144685" checksum="E2F8F161D9A32DA8" modified="2020-10-07" version="1">MSVIVAQALHIFGFQSRVANNICFFDEQIIVYPAGNSCVKYHIEQKWQKFIPGSEKSQGMIALAISPNRRYLAISETLQDKPAITIYELSSVPCKKRKILNIFDFTVQEFISLAFSPDSKYLVAQAGPPESQLAYWMWEKQRTMAIIRVDIQNNPLYQVSFNPQDNTQVCVIGNGIFKLYKYTEGTLKQTNFIRGEPQNYLSHAWLSEDKVIVGSDTGKLFLFDSGDLRWETSIERKETTTDMSHEEPAAESESLIEFPVTNSPEYSTEAVSDTEGQQQAFLPRVTAIAAYSKGFACSAGPGIVLLFEKSDEKDGYRESREIRIPQDPCSSDPNQSDKQDVVCISFSPAEETLLISTNKNQVYYLSMSSAELTKGETAHFEYLNFPIHSSAVTGLDTCIRKPLVATCSLDKSVRIWNYETNTLDLYKEYQEEAYALAFHPGGLCVLVGFADKLRLMNLLIDDIRTFKDFTVRGCRECTFSTSGHLFAAVNGNVIHIYSTISFDNVINLKGHNGKVRSVVWSMDDYKLVSCGTDGAVYEWNLQNGKRESECVLKSCSYSGVAISPDAKVIFAVGSDHSIKEICDSSILREVPSFDVTYTCIVISHNGRMVFTGTSAGTIRSMKYPLPLHKEFNEYQAHAGAVTKISITSDDQFLLTSAEDGCIIIWKVYDKEGRGLKREREVPYSDEVLITRTDMEEKTQVMLELKTRVEELKMENEYQLRLKDMNYSEKIKELTEKFIQEMESLKTKNQVLQTEKEKQEIQHQDQLSELIEKQSREVQDLESGNNQKLLLEYEKYQELQMKSQRMQEEYEKQLHEMEENKSQALEELTEYYEAKLLEKGTLLEEAQDDARQQLREFEETKKQIEEDEDREIQDIKIKYERRLREERESNLRLKGETGIMRKKFNSLQKEIEERCSDIEAMKLEQQKLQGIIKALEKDIMGLKREIQERDETIQDKEKRIYDLKKKNQELEKFKFVLDYKIKELKKQIEPRENDIKAMKEQIQEMEGELEHFHKQNTQLELNIAELRLKLRTTDREMHKEIQKERDMEALVKRFKTDIHNCIGFIQEPKKLKESIRDIYDKYVQKADRVEIVGVDSDLHQEYTRQREHLEHNLATLKKKVVKESEIHRADYVRIMQENVSLIKEINDLRRELKVTRIQVHDLESTLSLLRKHKKSTAQPSGPSPDRQLSPGLQRLNLEEETERIIQMQRLEIKCLRDQMQGQEKALAIRPLSSGRLPILEMDKISGIQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2U5</accession>
    <name>A0A6I9Y2U5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Homeobox protein Hox-B6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HOXB6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917468.1">
        <property type="nucleotide sequence ID" value="XM_014061993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2U5"/>
    <dbReference type="GeneID" id="106545441"/>
    <dbReference type="KEGG" id="tsr:106545441"/>
    <dbReference type="CTD" id="3216"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000017">
        <property type="entry name" value="Homeobox protein antennapedia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF9">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="146"/>
            <end position="206"/>
        </location>
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    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="148"/>
            <end position="207"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013917468.1"/>
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    </evidence>
    <sequence length="215" mass="24677" checksum="FAF4F6278694CED6" modified="2020-10-07" version="1">MSSYFVNSTFPVSLAGGQEPFLGQLPLYSTGYADPLRHYPSTYGTGGGGVQEKGYAAAPYYQQTNGAYNRSPACDYGTSGFYREKETGCSLSSLEEPVQFGQEQARKSECSQSKHVFGDNEDQKCSTPVYPWMQRMNSCNSSSFGPSGRRGRQTYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKENKILVNPCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3B6</accession>
    <name>A0A6I9Y3B6_9SAUR</name>
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        <submittedName>
            <fullName evidence="8">Complement C1q tumor necrosis factor-related protein 5-like</fullName>
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        <name evidence="8" type="primary">LOC106540730</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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            <taxon>Eukaryota</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Secreted</location>
        </subcellularLocation>
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        <property type="entry name" value="Collagen/C1q_domain"/>
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        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
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        <property type="entry name" value="COMPLEMENT C1Q TUMOR NECROSIS FACTOR-RELATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
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    </dbReference>
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        <property type="entry name" value="Collagen"/>
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        <property type="entry name" value="COMPLEMNTC1Q"/>
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    <sequence length="239" mass="25663" checksum="72A754066ED2CD04" modified="2020-10-07" version="1">HPGRVNPAGREFQGCQGCPAARRTSWGSCCLPTEPPPWPGRGGKERGSASAPCPRGLQGEKGEIGPPGLTGTRGESGSPGTNGLPGEKGPQGECAVAPRSAFSAKRSESRSPPLPDVPIVFDIILINEQGHYDGNTGKFMCEIPGLYYFAVHATVYRTSLQFDIMKNGKSVASFFQFYGNWPKPTSLSGGSLVRLEPEDEVWVQVAVGEYTGFYSSVKTDSTFTGFLVYSYWQNSPVFA</sequence>
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    <accession>A0A6I9Y414</accession>
    <name>A0A6I9Y414_9SAUR</name>
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        <submittedName>
            <fullName evidence="8">CDGSH iron-sulfur domain-containing protein 3, mitochondrial</fullName>
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        <name evidence="8" type="primary">CISD3</name>
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                <consortium name="RefSeq"/>
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            <name>[2Fe-2S] cluster</name>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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        <source>
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    <sequence length="129" mass="14304" checksum="D47A8E30E167864C" modified="2020-10-07" version="1">MPGLQKARGLVTSPLGSFCRFFFYQIRSCSAPSASSVRPVIAAKEPFPVDLIAEKRYAWCACGHSQKQPFCDGTHKKNAPEMSSLKFTAGESKKAWLCGCKYTKNPPYCDGTHKEDFVQKADLHSQPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4M7</accession>
    <name>A0A6I9Y4M7_9SAUR</name>
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        <submittedName>
            <fullName evidence="3">Dentin matrix acidic phosphoprotein 1</fullName>
        </submittedName>
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        <name evidence="3" type="primary">DMP1</name>
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    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
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            <taxon>Colubroidea</taxon>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="DMP1"/>
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        <property type="entry name" value="DENTIN MATRIX ACIDIC PHOSPHOPROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="DENTIN MATRIX ACIDIC PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07263">
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    </dbReference>
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        <location>
            <begin position="14"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919043.1"/>
        </source>
    </evidence>
    <sequence length="708" mass="79633" checksum="B92E857D43D3E019" modified="2020-10-07" version="1">MDYAVSRFLISPADTMDTFPPISISVEQGLDKRPDSTDLSEEDAGNRLSSSTEDRRDLGDNSCESCESNSFPREVSSSSIESFSKDDHEWSTQDNEDGGYFGHQMVYRKRADNKDSHYHRKEINKESSDDESAGRMSLEDQSEGRLLGANKISKEQFDVENDGMVYTPEQVLYTFVEKGGSDLTAEDISGDDSLDGNNDEDPGHTISPSEVDALQPPAPHGEQDGGHTGGRHSDDSSSSSSSSSESRNLEREDNHVIDYGRRHGGDSYSSSQEDGYDFQSEEMQGDEPMTFESHGSDSHMLPEAIHSKESSQEMHSSSKEVEHLEKKAYHYVDGDSDEDNSPEHEQNKSSKEDDDNSEVDSQLVEDVSQSTENVPSQSREDETSHSGQSVESSSRGQSRENLNSHSREDIHSESREEDKHSQSREHDKHSRSREDDKHSRSTEDDKNSQSTEDDKHSWSKEDATRGHSQSQSKENRSSHSREDKRSQSQEDDVHSQSIELASQSRENEDTVSEEDVKSDSTEDTRSSQEKVVQKSKETEEESNEHNPSESQESHSVEKSENMPPRPAESREFKRHQSRSSEKSSSMEESEESAEDTTESVEDDSRLSQSASSESSKVSEESASSDDSNSNEGQDQQSRSSETSESKEEESNSIERPSQSREDTRSQSVSMEDSSWSRSKELESRKLMLDFYHNKPFNDYDDNDCQDGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4Y7</accession>
    <name>A0A6I9Y4Y7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-binding protein 5</fullName>
            <shortName evidence="4">SH3BP-5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106546890</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Functions as guanine nucleotide exchange factor (GEF) for RAB11A.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with GDP-bound and nucleotide-free forms of RAB11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Colocalizes with RAB11A on cytoplasmic vesicle membranes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal half of the protein mediates interaction with RAB11A and functions as guanine nucleotide exchange factor. Four long alpha-helices (interrupted by a central kink) assemble into coiled coils, giving rise to a 'V' shape.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SH3BP5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919353.1">
        <property type="nucleotide sequence ID" value="XM_014063878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4Y7"/>
    <dbReference type="GeneID" id="106546890"/>
    <dbReference type="KEGG" id="tsr:106546890"/>
    <dbReference type="OrthoDB" id="446789at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007940">
        <property type="entry name" value="SH3BP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423:SF8">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05276">
        <property type="entry name" value="SH3BP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="360"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="189"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013919353.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="43948" checksum="A681C1E4F59903C5" modified="2020-10-07" version="1">MLRAGKEIHQQTRSSPAQQTGEWNRKPDMGDKVFGKLHYAAFNVYCYPKIHMTKSTLSSQEKQQHYCSECPVWLRSQAAGLKQYALDRKQLVFDVPAFLEEEKKLGNQPIGNYSPDKIPCQHCSQKQKWIEIQESVFCVWFISSQHTLGEKQLKTMQKNVAGPKAQCKKWLPGLTEDSKAGCSICSVSRDSSALQVNEAEEERLRSEREHQRVTQLCQQAEAKVQSLQKSLKRVILKSKPYFELKAQFNQILEEHKARVTSLEQQVSQAKMRYSVALRNLEQISEQIHARRLQRLVGRRASPVGAEASPAILYGGMVLPPEPVTSSDTLSVLSFQTIASDLQKFDSVEHLMGLSDAASLNSDELEEREQRRMAQPHSFKHHRSISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y588</accession>
    <name>A0A6I9Y588_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Fez family zinc finger protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FEZF1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912930.1">
        <property type="nucleotide sequence ID" value="XM_014057455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y588"/>
    <dbReference type="GeneID" id="106541894"/>
    <dbReference type="KEGG" id="tsr:106541894"/>
    <dbReference type="CTD" id="389549"/>
    <dbReference type="OrthoDB" id="5062908at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000103">
        <property type="entry name" value="FEZ family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000251">
        <property type="entry name" value="FEZ family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000227">
        <property type="entry name" value="fez family zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000164">
        <property type="entry name" value="Fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000194">
        <property type="entry name" value="Fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000863">
        <property type="entry name" value="fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="443"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013912930.1"/>
        </source>
    </evidence>
    <sequence length="477" mass="51259" checksum="E1C4AF6DAEE729B2" modified="2020-10-07" version="1">MEKSRQQQQQQFSPTAAKTLAVARGNMLSAPKPLAFSIERIMARSGPEPQAVPLLPHFLQGGGGTPKAQQQPAFSLGPSIPCMIPFVPLAYEPFAKVGSEPRKPPLEPPSSSSSSSASSALSCALSLKPQAGEPSLSLPQPPPHYKLVRPRVIKHSSFHALGALSYFSRASEPQPQPPGLSLHPVASYFLGSSLPAPALQDSTGPKSYLAERNKLQALPSGPEKFSQAAFKDLAQAQLHNAAAAAAAAAAHLLASEKLPFKGVSADFSRGSPSAKPKVFTCEVCGKVFNAHYNLTRHMPVHTGARPFICKVCGKGFRQASTLCRHKIIHTQEKPHKCNQCGKAFNRSSTLNTHTRIHAGYKPFVCEFCGKGFHQKGNYKNHKLTHSGEKQFKCTICNKAFHQVYNLTFHMHTHNDKKPFTCPTCGKGFCRNFDLKKHVRKLHDNGGGGGAPLGLPRGGGGGGGSETDLPPPPLLHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5B1</accession>
    <name>A0A6I9Y5B1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">Glutamine synthetase</fullName>
            <ecNumber evidence="6 18">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GLUL</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16 17">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="6 18"/>
    <dbReference type="RefSeq" id="XP_013919658.1">
        <property type="nucleotide sequence ID" value="XM_014064183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5B1"/>
    <dbReference type="GeneID" id="106547127"/>
    <dbReference type="KEGG" id="tsr:106547127"/>
    <dbReference type="CTD" id="2752"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF45">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="19">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="20">
        <location>
            <begin position="113"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013919658.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="41903" checksum="F60B56B94E9CC693" modified="2020-10-07" version="1">MATSASSHLSKTIKQMYMKLPQGDKVQAMYIWIDGTGEFLRCKTRTLEQEPKNVEDLPEWNFDGSSTFQSEGSNSDMFLVPSAMFRDPFRKDPNKLVLCEVLKYNRKPAETNLRSTCRRIMDMVSNQIPWFGMEQEYTLLGTDGHPFGWPSNGFPGPQGPYYCGVGADKAYGRDVVEAHYRACLYAGVNIGGTNAEVMPAQWEFQVGPCEGVDMGDHLWIARFILHRVCEDFGVIVSFDPKPIPGNWNGAGCHTNFSTKGMREEGGLKLIEDAIEKLGKRHQYHIRAYDPKGGLDNARRLTGFHETSNIHEFSAGVANRGASIRIPRNVGQVKKGYFEDRRPSANCDPYAVTEALVRTCLLSETGDEPFEYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y631</accession>
    <name>A0A6I9Y631_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Major histocompatibility complex class I-related gene protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106542452</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913660.1">
        <property type="nucleotide sequence ID" value="XM_014058185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y631"/>
    <dbReference type="GeneID" id="106542452"/>
    <dbReference type="KEGG" id="tsr:106542452"/>
    <dbReference type="OrthoDB" id="8936120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF242">
        <property type="entry name" value="MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-RELATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0490">MHC I</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="124"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013913660.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="27745" checksum="728A2A71A6AE653B" modified="2020-10-07" version="1">MRKASKRVNRQYWQPEHRAPCFIPTLHLNHGLHTFQRMYGCELLADGSKRGFLQYGYEGRTFITFDKETLTWVAPDPQAQIARRKWDAIPGKNQGHKFYLEEECIQWLEKYLSYGNETLLRTEPPVVTMSSRTEVEDGMETHICRVDGFYPRDIDASWRRDGEEWLEETFHGSVAPNADGTYHYWLSIRIDPKERSHYRCHVEHDGLQEPLDLELKGKSCREGKAGLSGRLEGGGREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6K3</accession>
    <name>A0A6I9Y6K3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Atypical chemokine receptor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ACKR3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920758.1">
        <property type="nucleotide sequence ID" value="XM_014065283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6K3"/>
    <dbReference type="GeneID" id="106547990"/>
    <dbReference type="KEGG" id="tsr:106547990"/>
    <dbReference type="CTD" id="57007"/>
    <dbReference type="OrthoDB" id="5963140at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019956">
        <property type="term" value="F:chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000141">
        <property type="entry name" value="atypical chemokine receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001416">
        <property type="entry name" value="ACKR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047143">
        <property type="entry name" value="GPER1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226:SF5">
        <property type="entry name" value="CHEMOKINE (C-X-C MOTIF) RECEPTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182 AND ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00646">
        <property type="entry name" value="RDC1ORPHANR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="65"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013920758.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="41551" checksum="A1B1D4A3E70D44D1" modified="2020-10-07" version="1">MNTIDMPPIFNLLEVENLNETNVTCNNGECITVDSLLCPNVINKSALLYTLSFLYIFIFMIGLVANFVVVWMNFQTKITGYETHLYIFNLAVADLCVIITLPIWVVSFVQHSQWNMGEISCKIAHLVFSINLYGSIFFLTCMSVDRYVSVAYFQPASSVKKKRLRCCICILVWLFAFFAALPDTYYLTTVSSNNETYCRPVYPEETAKDWLAGIELTSVILGFVIPFPLIALSNCLLATTVLASNDQERNSNVKIIFSYVLVFLVCWLPYHIVVLLDFLLAFHFIPFSCQMENFLYAALHVTQCLSLVHCCINPILYSFINQNYKYELMKAFIFKYSAKTGLTKLIDTSKISETEYSALEQNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y929</accession>
    <name>A0A6I9Y929_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA-binding protein PNO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">RNA-binding protein pno1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PNO1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNO1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917955.1">
        <property type="nucleotide sequence ID" value="XM_014062480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y929"/>
    <dbReference type="GeneID" id="106545821"/>
    <dbReference type="KEGG" id="tsr:106545821"/>
    <dbReference type="CTD" id="56902"/>
    <dbReference type="OrthoDB" id="1932641at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22391">
        <property type="entry name" value="KH-I_PNO1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22392">
        <property type="entry name" value="KH-I_PNO1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000009">
        <property type="entry name" value="RNA-binding protein PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000048">
        <property type="entry name" value="RNA-binding protein PNO1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055212">
        <property type="entry name" value="KH-I_PNO1_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="149"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013917955.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="27487" checksum="B0B7A45CC6E77B9C" modified="2020-10-07" version="1">MEMESSGEERLPTEMETHSPQEGFTTVLKRKRRREPQMDTNESPVPRKRPDFPPLSGEALACGKGELRKIPVPANRYTPLKENWMKIFTPIVEHLQLQIRFNLKTRNVEIKTCKETKDIGALTKAADFVKAFILGFQVEDALALIRLDELFLESFEVTDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADSKVHILGSFQNIKMARTAICNLILGSPPSKVYGNIRAVASRTAERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9I5</accession>
    <name>A0A6I9Y9I5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleolar protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106546395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918735.1">
        <property type="nucleotide sequence ID" value="XM_014063260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9I5"/>
    <dbReference type="GeneID" id="106546395"/>
    <dbReference type="KEGG" id="tsr:106546395"/>
    <dbReference type="OrthoDB" id="10047222at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013918735.1"/>
        </source>
    </evidence>
    <sequence length="145" mass="15558" checksum="DDC6B6AC65D9E6A9" modified="2020-10-07" version="1">MRLEIYQTSQDEPVTLEKQQSRDSSASVTHSSYSLPASSFSQDPVYINGSLNYSYRSYGTLGGALQPTPSLQTGNHSNGPTDLSMKSGTSSTTPPANSTNRGVPAAQLSPTEISAVRQLIAGYRESAAFLLRSADELENLILQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAH0</accession>
    <name>A0A6I9YAH0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase delta subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">POLD2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920410.1">
        <property type="nucleotide sequence ID" value="XM_014064935.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547697"/>
    <dbReference type="KEGG" id="tsr:106547697"/>
    <dbReference type="CTD" id="5425"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07387">
        <property type="entry name" value="MPP_PolD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.430:FF:000001">
        <property type="entry name" value="DNA polymerase delta subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041863">
        <property type="entry name" value="PolD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0228">DNA excision</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="8">
        <location>
            <begin position="49"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="7">
        <location>
            <begin position="200"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013920410.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="48086" checksum="56916583B892A2F6" modified="2020-10-07" version="1">MFSEQGIQSAQGLLTSPSAVASTFARVPISTYTNCSQNFRLGERSFNRQYAHIYATRLIQMRPLLVDRARRKWGSNITVKKLCELQISEKCCVVGTLFKCMQLQPSILREISEEHNLPPHPPRLKYIHESDELILEDELQRIKLEGEIPIDKLVTGTILAVYGSEKDDGRFLVEDHCFADMPTQLPMMGPNTDKXGPYNLVLGRPLLKQSRALRMQAKYLTKKTQAASVEAVKMLDEILLQLCMSIPVDVMPGEFDPTNYTLPQQPLHRCMFPLSNAYTTLQLVTNPYQANIDGVRFLGTSGQNVSDIFKYSSMSDYLEILEWTLRIGHLSPTAPDTLGCYPFYKTDPFIFSDCPHIYFCGSAPSFKSKEVKGEDGQKVLLVTLPDFSSTKTACLINLCDLTCQPVTFSAFGDDDDEEEETVMETAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAN2</accession>
    <name>A0A6I9YAN2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NHL-repeat-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NHLRC4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920720.1">
        <property type="nucleotide sequence ID" value="XM_014065245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAN2"/>
    <dbReference type="GeneID" id="106547960"/>
    <dbReference type="KEGG" id="tsr:106547960"/>
    <dbReference type="CTD" id="283948"/>
    <dbReference type="OrthoDB" id="10020332at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF50">
        <property type="entry name" value="SMP-30_GLUCONOLACTONASE_LRE-LIKE REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="NHL" evidence="2">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="2">
        <location>
            <begin position="258"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920720.1"/>
        </source>
    </evidence>
    <sequence length="363" mass="40312" checksum="63542754541AAB00" modified="2020-10-07" version="1">MDYTLEDTQRKEKLLKTVYTLQVKSDSTFQKASPLLTSHPGMGMVTDQQVHQLVEKARIICHDLDDIKNHLYYRQDDLVRRVSNPNRNHYGAVGGIHCSPDGVVYLTTENAPRVDLLNRSGQVFRSIPCVEWKREVFLPEDVTLTRAGMVAVTDMLNGTVHVFNDRSMFSKGAWLRIGNFRSPRGIAVDTSGRILVADYTEGKVHIFALDRAFKMQNAHSISNLSGPRYVCSTPDGGFAVSEECGEVKLFDSSHKLVSSVSSKYGHRFGNPAGLCADKEGNIIVADEQRQKIHLFPQNGSAICLVSKGLLRPTSVACAIDGSLFVTDSRANDIKVFKYRVRPHYIPDIIPADNPSYSPRGKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAX3</accession>
    <name>A0A6I9YAX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">T-cell acute lymphocytic leukemia protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TAL2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921175.1">
        <property type="nucleotide sequence ID" value="XM_014065700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAX3"/>
    <dbReference type="GeneID" id="106548346"/>
    <dbReference type="KEGG" id="tsr:106548346"/>
    <dbReference type="CTD" id="6887"/>
    <dbReference type="OrthoDB" id="10069510at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19707">
        <property type="entry name" value="bHLH_TS_TAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000015">
        <property type="entry name" value="T-cell acute lymphocytic leukemia 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040238">
        <property type="entry name" value="TAL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864:SF21">
        <property type="entry name" value="T-CELL ACUTE LYMPHOCYTIC LEUKEMIA PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864">
        <property type="entry name" value="T-CELL ACUTE LYMPHOCYTIC LEUKEMIA/STEM CELL LEUKEMIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="93"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921175.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="24033" checksum="66E45885835F1BCA" modified="2020-10-07" version="1">MKRISQEMWNKLPGSATLVLTRARQRSPDSINETAGWDWHLAGLSGKRLWRFEAERPESCEEETLADFSCCEYFSTARFSCLFGKVLPILRMTRKIFTNSRERWRQQNVNSAFAKLRKLIPTHPPDKKLSKNETLRLAMRYINFLVKVLGEQGLPQTGVNPRERILGLFQQNPHLEHMEELTLLEDSEVPSPNTSSNIPECWSETSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBD7</accession>
    <name>A0A6I9YBD7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Calcium-transporting ATPase type 2C member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ATP2C1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921415.1">
        <property type="nucleotide sequence ID" value="XM_014065940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBD7"/>
    <dbReference type="GeneID" id="106548547"/>
    <dbReference type="KEGG" id="tsr:106548547"/>
    <dbReference type="CTD" id="27032"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000017">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="11">
        <location>
            <begin position="248"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921415.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="48326" checksum="51455A616076E237" modified="2020-10-07" version="1">MKGAYEEVIRYCTTYNNKGHSLPLNQQEREHYQHEKASMGSAGLRVLALASGPELGQLSFLGLVGIIDPPRTGVKEAVAALITSGVAIKMITGDSQETAVAIASRLGVYSKSSQSISGEEVDALDIQQLSQIAPKVAVFYRASPRHKLKIVKSLQNNGEVVAMTGDGVNDAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMFAIEEGKGIYNNIKNFVRFQLSTSIAALTLISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRNVKDSILTKNLIIKILVSSIIIVCGTLFVFWRELRDNVITPRDTTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCSNKMFCYAVLGSIMGQLLVIYFPPLQKVFQTESLSITDLQFLLGLTSSVCIISEIIKKVEKSREKTQKHGSSQHLGSFLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBW5</accession>
    <name>A0A6I9YBW5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106548687</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921590.1">
        <property type="nucleotide sequence ID" value="XM_014066115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBW5"/>
    <dbReference type="GeneID" id="106548687"/>
    <dbReference type="KEGG" id="tsr:106548687"/>
    <dbReference type="OrthoDB" id="9975554at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15231">
        <property type="entry name" value="7tmA_OR5V1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921590.1"/>
        </source>
    </evidence>
    <sequence length="307" mass="34981" checksum="BEF610114B171554" modified="2020-10-07" version="1">MMVHNETRLTEFIILGFRNLQEIHLLLFTGFFIIYLFTVVWNTFIILMTVMDQKLQNPMYFFLGNLSFLDICYTSTTIPQMLAHLLSEKNSISYIGCALQAYFFFSFVGIECLLLAVMAFDRYVAICNPLRYTLIISKEICLQLATACWTCGFLNSVVHTTFAFQLPFCGNNHLDAFYCDIAPLLRLSCGDTSLNQVLLLSIGFFIAWTPLACILLSYGYIISTILSMHSSEGRQKVFSTCSSHITVVLLYYGSALFTYSRSNNNNSSNQAKLIPVIYSILTPLLNPIIYTLRNKDVMNAWRNVINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEH6</accession>
    <name>A0A6I9YEH6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tight junction protein ZO-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TJP1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922405.1">
        <property type="nucleotide sequence ID" value="XM_014066930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEH6"/>
    <dbReference type="GeneID" id="106549310"/>
    <dbReference type="KEGG" id="tsr:106549310"/>
    <dbReference type="CTD" id="7082"/>
    <dbReference type="OrthoDB" id="418634at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090557">
        <property type="term" value="P:establishment of endothelial intestinal barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905605">
        <property type="term" value="P:positive regulation of blood-brain barrier permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150105">
        <property type="term" value="P:protein localization to cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12026">
        <property type="entry name" value="SH3_ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035597">
        <property type="entry name" value="ZO-1_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865:SF25">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="73"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="474"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="561"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="352"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="571"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="734"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="775"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013922405.1"/>
        </source>
    </evidence>
    <sequence length="788" mass="88406" checksum="072C2D953199ECC3" modified="2020-10-07" version="1">MSSFSRECRLRLTRGAPRSFECVSLGMPPQALLDQLVGDLQSHLDEMERLRVGLLDAYPRLSVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREGRPLSAGYELWLWLNSRASPPHSWLFPSLPCLSLATINLNSLNDGWYGALKEAIQQQQNQLVWVSEGKADGTTSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREEAPGVHPESQTFPPYGSQGQAQPNHRIDSPGFKNNTAQPKAEASSPPYLSPSPETGGPASSMPAANPCVSLPNVRLEEAGAPPYNSYQPQAGPLRMAGPEAPHIVLRDPEMPSLPPHLDSTKMYRKEPFVNEDLPRQNFALKQPGQRQEKDPNLTYELQGPYPEKPANRDYEPLAYRYDSSNYVDPFPHGYDPRLPYEERVPPYEDHWMYYEEKQPYQPRTPYESQPPRDFDPRANAEESAARSGYYAGQPHFEDAPPVAYDSRPHYEPGPKAFNLSQLRYEEPPPPGYEVHGRYKSEPQPYSATVPRSPEPKHYYESQPRGYEQAPPPGFSTKAAPYDASLGSGGAHPALPPQGKPEAMPCNSKALPTPPTEEEDDPAMKPQSVLTRVKMFENKRSSSLEKIKDSNDVSAVKHHPELAPKPNVAPLTGPKSASQNPSDKSAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEK6</accession>
    <name>A0A6I9YEK6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Chromatin assembly factor 1 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Chromatin assembly factor I p60 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20 21 22" type="primary">CHAF1B</name>
    </gene>
    <organism evidence="19 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922600.1">
        <property type="nucleotide sequence ID" value="XM_014067125.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922601.1">
        <property type="nucleotide sequence ID" value="XM_014067126.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922602.1">
        <property type="nucleotide sequence ID" value="XM_014067127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEK6"/>
    <dbReference type="GeneID" id="106549466"/>
    <dbReference type="KEGG" id="tsr:106549466"/>
    <dbReference type="CTD" id="8208"/>
    <dbReference type="OrthoDB" id="71227at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000248">
        <property type="entry name" value="Chromatin assembly factor 1 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003156">
        <property type="entry name" value="Chromatin assembly factor 1, subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029129">
        <property type="entry name" value="CAF1_p60_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15512">
        <property type="entry name" value="CAF-1_p60_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="18">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit B C-terminal" evidence="17">
        <location>
            <begin position="418"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="404"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="502"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF15512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013922600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013922601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013922602.1"/>
        </source>
    </evidence>
    <sequence length="542" mass="60159" checksum="B38C4C0042D9669E" modified="2020-10-07" version="1">MKVITCEIAWHNKEPVYSLDFQHGTDGKINRLASAGVDTTVRIWKVEKGPDGKAIVEFLSNLARHIKAVNVVRFAPSGEILASGGDDATILLWKLNDNKELEAVVFQDDESDNQLNKENWTVIKTLRGHLEDVYDICWTPDGNYMASASVDNTAILWDVSKGQKVSIFNEHKSYVQGVTWDPVGQYIATLSCDRILRVYNTQTKRVAFNVTKMSSGGGSEGEVKSYRMFHDDSMKSFFRRLTFTPDGSLLLVPAGCVESGENVMNTTYVFSRKNLRRPIAHLPCPGKATLAVRCCPVYFELRSACNKEESSQKIGLTNLPYRLVFAVASEDSVLFYDTQQTFPFGYVSNIHYHTLSDISWSSDGSFLAISSTDGYCSFVTFEEGELGIPLKNKPAINIKTPSTAEKKIKKGQSPSVVSPVSKPVDGSLNKSGEPSTACQTPTNSKDLHCTPVAVKNISMSSSEDKKTVQASIHTTKVNQPRRITLNTLQAWNKSPRRVALIPLKEATPDTSRNAIQHERPSPPEDQLQNPPSPKRLRMDETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEV2</accession>
    <name>A0A6I9YEV2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Single Ig IL-1-related receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SIGIRR</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922520.1">
        <property type="nucleotide sequence ID" value="XM_014067045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEV2"/>
    <dbReference type="GeneID" id="106549400"/>
    <dbReference type="KEGG" id="tsr:106549400"/>
    <dbReference type="CTD" id="59307"/>
    <dbReference type="OrthoDB" id="6075577at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000011">
        <property type="entry name" value="single Ig IL-1-related receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF19">
        <property type="entry name" value="SINGLE IG IL-1-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="6">
        <location>
            <begin position="153"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013922520.1"/>
        </source>
    </evidence>
    <sequence length="404" mass="46585" checksum="414E2B4A7953FEC4" modified="2020-10-07" version="1">MLPHFLYPSSNLTMEQALDSQITLNCTVQLPFSNDCDLDLRWMKDNQFLDNKTHSSYTQWFSDNKTKIFISSSLELNMTDENYGIFACFIRNSTALFTLKKPEETVGHLGAVLATFFAAALLLLVAIMYVKCRLNALLWYRNHYGEVEINDGKIYDAYVSYADSVDDRKFVNFIVKPQLENRYGYKLFLDEKDILPNSEPSADLIMNVSRCRRLIVVLSYAYLEQEWCNNFREGLWRLLELSRKPIFIVFESQYRDIAHPAINLLKQHKSNVTLLMWHAGSMAIASSFWKELCLALPRKVSYQGLRGDPQTFLQEDKDPMLILQSNYPEFQGDSHPEGDIVIRTPIIKNLPPPVKIDPDGPCLGAMEEVQLEDSQKNEIDISDLGSRNYDARTDFYCLVTEDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFP2</accession>
    <name>A0A6I9YFP2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pleckstrin homology-like domain family A member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106549788</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923006.1">
        <property type="nucleotide sequence ID" value="XM_014067531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFP2"/>
    <dbReference type="GeneID" id="106549788"/>
    <dbReference type="KEGG" id="tsr:106549788"/>
    <dbReference type="OrthoDB" id="9630709at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042832">
        <property type="entry name" value="PHLA1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15478:SF4">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN FAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15478">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN, PQ-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="9"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923006.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="17317" checksum="55444239138E32A8" modified="2020-10-07" version="1">MLESGGGKPVKEGVLEKRSDGLLQLWKKKRCILTEEGLLLIPPKQEPAAAAASPPPAAHGETAVAAAKIKQLPFSTMKTVDCVERKGKYMYFTVVMAEGKEIDFRCPQDQGWNAEITLQLVQYKNRQAILAVKSSRQKQQHLVQQLGTRLRSSSNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFQ2</accession>
    <name>A0A6I9YFQ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106549646</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922824.1">
        <property type="nucleotide sequence ID" value="XM_014067349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFQ2"/>
    <dbReference type="GeneID" id="106549646"/>
    <dbReference type="KEGG" id="tsr:106549646"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF122">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 27 SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013922824.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="20487" checksum="8EA80EB44FEC633A" modified="2020-10-07" version="1">MSFLSKALKMDCKQVIETQRNYIYSVVFGNKFHQLPKLSGSTSLEMQNNAAAAAATEDKEEEMVETSEEAKGLLKPSQHPSRRRVKSLKEMPGPKTFSNLMEFFCKDGFARIHEIQQKHVQEYGKIFKSHFGPKLVVSIADKDMVAHVLREEGCMPQRADMDSWQEYRTLRARATGLISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGR7</accession>
    <name>A0A6I9YGR7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 37-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923382.1">
        <property type="nucleotide sequence ID" value="XM_014067907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGR7"/>
    <dbReference type="GeneID" id="106550113"/>
    <dbReference type="KEGG" id="tsr:106550113"/>
    <dbReference type="OrthoDB" id="421075at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="48"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923382.1"/>
        </source>
    </evidence>
    <sequence length="546" mass="60840" checksum="678F26796D4AB1DA" modified="2020-10-07" version="1">MLLLFSQFKVLPIVEGVTDKSVFFLGVGNYALAQHAFIKSIQADQINVVAWTNLGVLYLTSGKIEQAHEAFKVAQSLEPSHLTCWIGQAFVAETVGSYETMDLFRHTTELSMHTEGAKGYAYWVCSTLQDKTNRNTELYKYNILQMNAIPTAQVALTKYTERIQNDACAFTMLGYLNEYLQLKKQAADTYQRAVLLLTNSEDRERYNIVMRNYGRSLCALGRCDDAIKAYLTTHLIEFDDIVGIALSYFKKGHFQESQKAYEKALAMAESEQDKAYILTALAIIEYKQNRVDAAKTLLFKCSILQEPNMESLQALCSLGLIKQDATLATAALKELLKHPGKKDNVYKRCLLASAVYALQGRHLAVQRQVSKAVHSNPDNPALWSLLSRLVPRYVPQNAKGGAVAGNIACILDLNHGKKALLNTAVNQLATGCHKAENKKNILKAVHLSPDDPTAWAVLLAACQAENTIAHLHNKQSKQTHLEKMIVTAISAKTKAKELPASRAQEIEEWSFFQAITGLNEAGRTSEAEALCEKGLKKFPEQPMWYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHS3</accession>
    <name>A0A6I9YHS3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein CBFA2T2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CBFA2T2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923741.1">
        <property type="nucleotide sequence ID" value="XM_014068266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHS3"/>
    <dbReference type="GeneID" id="106550365"/>
    <dbReference type="KEGG" id="tsr:106550365"/>
    <dbReference type="CTD" id="9139"/>
    <dbReference type="OrthoDB" id="8872930at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000001">
        <property type="entry name" value="CBFA2/RUNX1 translocation partner 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1110:FF:000001">
        <property type="entry name" value="RUNX1 translocation partner 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1110">
        <property type="entry name" value="TAFH/NHR1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013289">
        <property type="entry name" value="CBFA2T1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013291">
        <property type="entry name" value="MTGR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014896">
        <property type="entry name" value="NHR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037249">
        <property type="entry name" value="TAFH/NHR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003894">
        <property type="entry name" value="TAFH_NHR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379">
        <property type="entry name" value="MTG8 ETO EIGHT TWENTY ONE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379:SF13">
        <property type="entry name" value="PROTEIN CBFA2T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08788">
        <property type="entry name" value="NHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07531">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01875">
        <property type="entry name" value="ETOFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01877">
        <property type="entry name" value="MTGR1PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00549">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158553">
        <property type="entry name" value="TAFH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51119">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAFH" evidence="13">
        <location>
            <begin position="84"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="12">
        <location>
            <begin position="478"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="361"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="548"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="417"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="551"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013923741.1"/>
        </source>
    </evidence>
    <sequence length="572" mass="63253" checksum="094256227956BB4C" modified="2020-10-07" version="1">MPGSPVEVKIQSRSSPSSMPPLPPVNSSGPRPVSFTPAALSNGMNHSPPTLNGAPSPPQRFSNGPASSSSSSLTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEKVRTLVLALVNSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHVLLNTNTASPADSSELLMEVNGNGKRHSPDRREENNFEREPIPSEPPAKRVCTISPAPRHSPSLMLPIINAAGQFHPTPPPLQHYTLEDIATSHLYRDASKMLEHRDLRERHSIGLNGGYQDELVDHRLTEREWADEWKHLDHALNCIMEMVEKTRRSMAVLRRCQEADREELNYWKRRCSEPTEPRKGGSDSAVRQHSPVSSDTINSESQRESSSRPGTAGYVTEEIWKKAEEAVNEVKRQAMSEVQKAVAEAEQKAFEMIASERARMEQTIADAKRQATEDAFLVINEQEESTESCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRICGQGLHSQVKPVSLSASRSSAATLNAVDGIASPALEKTSTTTSRSSTPASVTAVDTNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIV4</accession>
    <name>A0A6I9YIV4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ganglioside-induced differentiation-associated protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GDAP2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDAP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924283.1">
        <property type="nucleotide sequence ID" value="XM_014068808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIV4"/>
    <dbReference type="GeneID" id="106550823"/>
    <dbReference type="KEGG" id="tsr:106550823"/>
    <dbReference type="CTD" id="54834"/>
    <dbReference type="OrthoDB" id="365077at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd02905">
        <property type="entry name" value="Macro_GDAP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035793">
        <property type="entry name" value="Macro_GDAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF72">
        <property type="entry name" value="GANGLIOSIDE-INDUCED DIFFERENTIATION-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="2">
        <location>
            <begin position="43"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924283.1"/>
        </source>
    </evidence>
    <sequence length="493" mass="56019" checksum="689540742F52B355" modified="2020-10-07" version="1">MDPLGAPSNFVDINTLPGWSNYHNEDQSNSSHLPIKGGKVDVRSPFPYREDINAKIILWRGNVALLNCTAIVNTSNESLTDKNPVSESISMHAGPDLKVELQKLKGCRTGEAKMTKGFNLAARFIIHTVGPKYKSRYRTAAESSLYSCYRNILQFAKEQAMSSVGFCVINTVKRGYPLVDATHIALRTIRRFLEIHGESLEKVVFAVSDLEEPTYQKMLPLYFPRSLAEERESLPLLPEDIGNAEGEPVVPERQIRICKKPGAPEDNSDEEELEADFSFIGSHAFARMEGDVDKQRRLTLQGQLSEAALQKQHQRNYNRWLCRARAEDLSDIASLKALYQTGVDNCGRTVMAVVGRNIPVTLIDMEKALLYFIHVMDHIVAKEYIIVYFHTLTNVYNHLDSDFLKKLSDIVDVRYKRNLKALYFVHPTFRSKVSTWFFTTFTVSGLKDKVHHVEGLQQLFAAIPPEQIDFPPFVLEYDIRENGYYSYPSSPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ45</accession>
    <name>A0A6I9YJ45_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable lipid phosphate phosphatase PPAPDC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PPAPDC3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924378.1">
        <property type="nucleotide sequence ID" value="XM_014068903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ45"/>
    <dbReference type="GeneID" id="106550913"/>
    <dbReference type="KEGG" id="tsr:106550913"/>
    <dbReference type="CTD" id="84814"/>
    <dbReference type="OrthoDB" id="10266771at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03391">
        <property type="entry name" value="PAP2_containing_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF17">
        <property type="entry name" value="INACTIVE PHOSPHOLIPID PHOSPHATASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="3">
        <location>
            <begin position="145"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013924378.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="29889" checksum="7147ACD49A5F26D8" modified="2020-10-07" version="1">MPAVSNRARLARERSNVLNRTEFLSLSHPLKSAQEPRSLPRRSNSQGGPAPSQSEGSKEQRPSQQLPEEDCMQLNPTFKGIAYNSLLAIDISLSKRLGVCASNSAAWGNARSMVNLIGITGHGIPWIGGTLICLVKSSTLAGQEVLLNLLLALLMDIIIVAGLQKMAKRKGPYDVSPGILDYLTMDLYAFPAGHASRAAMVSKFFLNHLVLAVPLRILLILWASLVGLSRIMVGRHHVTDVLSGFFFGYIQFRMVEILWMSSNTCQMLISMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJE6</accession>
    <name>A0A6I9YJE6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mastermind-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924099.1">
        <property type="nucleotide sequence ID" value="XM_014068624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJE6"/>
    <dbReference type="GeneID" id="106550679"/>
    <dbReference type="KEGG" id="tsr:106550679"/>
    <dbReference type="OrthoDB" id="9908492at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007221">
        <property type="term" value="P:positive regulation of transcription of Notch receptor target"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046369">
        <property type="entry name" value="MAML1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15692">
        <property type="entry name" value="MASTERMIND-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15692:SF9">
        <property type="entry name" value="MASTERMIND-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924099.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="56917" checksum="D170495C93152308" modified="2020-10-07" version="1">MLSVPELRKSYNRNNGFYGKLQGSLKRKLVVNLTPGNNKRSNGIADNTFLDIKRIRGGENNLSMGRGQCGNSCPNPSVLGSFSVGQGAPRKMDNSTSNSHAGANGMFNMTLKEVKKEPGETISCSKHMDGQTSQNSVYPNQYGEDTGEQLMDPELQELFNELSNISVPPMSDLELENMINATIKQDEPFNIDIGQQIQRGPPAPSSSLQVDKMVIKQEYSPGLSQASVGSPQMRPPSTDSAYTMPGAAMSTSSPIPSVPQTQTPPSQGTSVPTRPLANWQEVSHAQQLKQIAANRQQHALIQQCQQSQSATSWPGLSSTEPSSGSFVQEKMSSPSFHQQQQQFSPQNSAMSSIPVNGTPSKGMNSYSYRPNSMPQGSSVNMAMPRKPQDIPRNYVGNNQVSLEESQNSIKPLFHFTSEQANKQISSGLGAPNKPSLLHYAQQPPQQLSNSAVQPQTSQQQQQPHSGQPQHLQRPPNVPLALQQKILLQKMLQNQPGAALQFLGPQNQMGNRSSRKKHHGDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YM77</accession>
    <name>A0A6I9YM77_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytosolic Fe-S cluster assembly factor NUBP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NUBP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925498.1">
        <property type="nucleotide sequence ID" value="XM_014070023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YM77"/>
    <dbReference type="GeneID" id="106551859"/>
    <dbReference type="KEGG" id="tsr:106551859"/>
    <dbReference type="CTD" id="4682"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002304">
        <property type="entry name" value="Nucleotide binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013925498.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24232" checksum="F6E491DAC794E5BC" modified="2020-10-07" version="1">MPKIMGLEGEQVHQSGSGWSPVYVEENLGVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEIDYLIVDTPPGTSDEHISIVQFLSSARVDGAVIITTPQEVALQDVRKEISFCRKVKLPVIGVVENMSGFVCPKCKKESQIFPLTTGGAEMMCRTLNVPLLGKMPLDPQIGKSCDRGHSFLAEAPHSPATLSCRNIIQRIQDYCTQNSPQEEDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMJ7</accession>
    <name>A0A6I9YMJ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fibronectin type-III domain-containing transmembrane protein C1orf233 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106551734</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925356.1">
        <property type="nucleotide sequence ID" value="XM_014069881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMJ7"/>
    <dbReference type="GeneID" id="106551734"/>
    <dbReference type="KEGG" id="tsr:106551734"/>
    <dbReference type="CTD" id="643988"/>
    <dbReference type="OrthoDB" id="9450734at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034446">
        <property type="entry name" value="Fndc10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39233">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39233:SF1">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17742">
        <property type="entry name" value="Fndc10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027023616" evidence="2">
        <location>
            <begin position="18"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="77"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925356.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="23193" checksum="88663D50E2B32434" modified="2020-10-07" version="1">MLAFSLLVALLCPGVWGTDPKARRAEGEGSGGREGGVAEPWCPYKVLSEDGPGRLCFRSPALGFHCAPERCTAHRSPGRALLANVLRNGSVLLQWAWPADQQPPLRGFALSCSWEGAYTRFQCESVRLGAACRDYLLADPHGSVRYRLCLQPVLGDGDEPAAVAECVEFTVEPAGMRDIVVAMTAVGGSICVMLVLICLLVAYLTENLTPSSAKRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMT9</accession>
    <name>A0A6I9YMT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">DNA excision repair protein ERCC-6-like 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12" type="primary">ERCC6L2</name>
    </gene>
    <organism evidence="8 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925091.1">
        <property type="nucleotide sequence ID" value="XM_014069616.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925093.1">
        <property type="nucleotide sequence ID" value="XM_014069618.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925094.1">
        <property type="nucleotide sequence ID" value="XM_014069619.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925095.1">
        <property type="nucleotide sequence ID" value="XM_014069620.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551503"/>
    <dbReference type="KEGG" id="tsr:106551503"/>
    <dbReference type="CTD" id="375748"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18005">
        <property type="entry name" value="DEXHc_ERCC6L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000019">
        <property type="entry name" value="DNA excision repair protein ERCC-6-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058052">
        <property type="entry name" value="DEXHc_ERCC6L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057931">
        <property type="entry name" value="RHH_ERCC6L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25806">
        <property type="entry name" value="RHH_ERCC6L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="96"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="472"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="931"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1036"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1125"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1400"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="937"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="951"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1046"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1130"/>
            <end position="1148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013925093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013925095.1"/>
        </source>
    </evidence>
    <sequence length="1498" mass="170372" checksum="D58EE27310051AAB" modified="2020-10-07" version="1">MSFETNGNGKCFASVSTGKNQEEKILTTKLSQFKTNKTTKKNLIFDDEDLEKPYFPDRSLPSPTVAFELSNNGEEVPYTINRYLRDYQREGVQFFHGHYVRKSGCILGDDMGLGKTIQVISFLAAVLKKKGTRADIENNMPDFLLRTMRKEMSSVPKKIFLIVAPLSVLYNWKEELDVWGYFKVIVLHGSRKEHELNRIKLGKCEIVLTTYDALRLYLNELNSLQWSAVIADEVHRIKNPKSQITQTMKALTCKVRLGLTGTILQNNMKELWCVMDWAVPGLLGSKARFKTEFSEPVELGQRHTATKRELATGRKAMTRLAQKLSGYFLRRTKALISDQLPKKEDRTVYCYLTEFQRAVYKTVLETEDVQLVLRAREACSCNSGHKRKNCCYKTNAHGETMNALYFSYVTVLRKIANHAALLQTENTSKQQEAYITRICSHVFSKFPDFVDLSKNAAFETLSDPKYSGKMKVLQKLLNHFRKNKDKVLLFSFSTKLLDVLEQYCMASGLDYRRLDGNTKAEDRVKIVKEFNSTEEVNICLVSTMAGGLGLNFVGANIVILFDPTWNPANDLQAIDRAYRIGQRRNVKVFRLISLGTVEEMMYLRQIYKQQLHFVAIGSKNAKRYFKAVQGSREHQGELFGIRNLFRLQDHGTCLTKEILEREGQVEAGVMTADTWLKEGSQPREETECDYEHQDTKPLEKEALGCSSDFSDEEHEEMSKVQATKKRNYKAKHDKVAEGQLSLLQCGFPKLLEKKTRTAKDGSCIRMAVDENADHRFMREQSKGKAQNDGSVKMEGRNISGCKKTLLSKMSVERQEKLDEEANRTYPVLPCCAEEKDQCKNIYNSNKNKDGIVMDTEDSSSENDIIFPSQFPNGQESIKKFQETDRNSDILAAKKMHLLKPLQKHLTSEESYTNVLPFSKIKPFENIRKTTARNEVSDVSDESEDIEMSISGKQKLTSTMKQKLFQNKERNKSSKTVVHRSSPSRKESNADSQIIEEYSSEEEHFPVKKVNASKKNLTLKGKRSGIQMMSRFSPHLNYPSMTLRTHSNSSRWESSVSKESPSGQGQKIGSLDGLFDNVAEVPYVHSNQHVVGASKAENKMSHWAVRDVFELQQFSQLPANIAVCRAKRSRQSPEDVSSKRKNVKQDHLTSETSRPPLLYITHPVHQKQKRVHRIGSITFLVGKTPRKIRRKQFEEMADLFHKTPVEEFAKYITQTTSEERQKMLKEFYSAKHPELKDILKVDVPASVSEGNKEGGKCKTKFGKKPSICNPGQLKQDLPIDLGVPGRSDAVEKEFQHKLSAQAPGGQNGEDKQLPADILEDSINGRNSLASKDFMASESLSSKSRIIERLSASSSKGQLNLKGTLSPQKRLMSDSASSQAEICQKNAVADLLGDTSILNDLFTSNENSPAERPRRPLNGPAEKAATRPKDFWDMLNEENEECLQRLTDVTAIEKLCERPSFTSLFKEKKESEKSLWKENDNFLWKIDKKPDTDDESFSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNN3</accession>
    <name>A0A6I9YNN3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="12">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">XRN2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013925882.1">
        <property type="nucleotide sequence ID" value="XM_014070407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNN3"/>
    <dbReference type="GeneID" id="106552193"/>
    <dbReference type="KEGG" id="tsr:106552193"/>
    <dbReference type="CTD" id="22803"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000003">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="15">
        <location>
            <begin position="326"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="410"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="871"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="871"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="909"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="951"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013925882.1"/>
        </source>
    </evidence>
    <sequence length="960" mass="110391" checksum="A6F53884AF07DC80" modified="2020-10-07" version="1">MGVPAFFRWLSRKYPSIIVSCTEEKAKECNGIKIPVDTSKPNPNEVEFDNLYLDMNGIIHPCTHPEDKPAPKNEDEMMVAIFEYIDRLFNIVRPRRLLYMAIDGVAPRAKMNQQRSRRFRASKEGMEGAEEKQRIRQEIMEKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIADRLNNDPGWKTLTVILSDASAPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLGLATHEPNFTIIREEFKPNKPKPCGLCNQFGHETKDCQGLPKEKQGEHDQFADCPPGMEQEFIFIRLCVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHLPSLEIREGAIDRLVNIYKNVVHKTGGYLTENGYVNLERVQMIMLAVGEVEDSIFKKRKDDEENFKRRQKDKRKRMKREQHTMLGGQYTPQPVGRGNRSSHQPISNPRHTAYEMRMQDRQSTTPSASPNSSFTSDGTPSPAGGIKRKMEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDINDKKFRRKVVQSYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMDSDFEKGTNPFRPLEQLMGVFPAASGNFLPPTWRKLMTDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRAALAQVYPNLTSEETRRNSLGSDVLFVGKHHQLLDFILEQYKSNEKEPVDIPPELCHGIQGKLTLNEYPILPDESVQSPVPLLRDLTQNSAISINFKDPQYQEDYIFKAVVLSGAKKPAAVLKPGDWEKTNNDGRSWKPQLGFNRDRRQVHLDQSAFRTLGHTMSRDRGQSGSGVYSNIAPPGAYNQGNAYRPLLGRPQHQIPKLLSNLRPQDSWQAPTPLFQQPPQRFDRNSGATPLLAWNRMPHSQGPPYQQNQYQGPGGPGGLMNYPQRPSDQRERGRQGYGRPYQLPPSSGRYNWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNS2</accession>
    <name>A0A6I9YNS2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">B-cell linker protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106552397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926133.1">
        <property type="nucleotide sequence ID" value="XM_014070658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNS2"/>
    <dbReference type="GeneID" id="106552397"/>
    <dbReference type="KEGG" id="tsr:106552397"/>
    <dbReference type="OrthoDB" id="10044490at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051751">
        <property type="entry name" value="Immunoreceptor_sig_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098:SF3">
        <property type="entry name" value="B-CELL LINKER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098">
        <property type="entry name" value="SH2 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926133.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="41945" checksum="F5A32D017CB2FEBF" modified="2020-10-07" version="1">MSKFKKFQNEQVALICKTGKNTWDRLTSKPPPSIPPVDYVPDRTEEEEQWSDDFDSDYENPDHHSDSEMYVVPTEENCDDSYEPPPSEHEPKMIPIPFTCAQGVYADDKVASNQKTWPSSIPPNQSASLSKKPATLPLLPPAVQKPKLPPKRMDDEADYVVPVDDEEDDEDNYIEPTEESTSQSEKPPAVNRSTKPPCSVPSASTQDVYEIPEEEQEPSPPLPRISKPLPPKPASRPSGGPSHERSIPKEPFASDTFKSDRNILPLPRHRPSPKADPQVLGAQTDGNNGVNNAAEFKIPPVNVPSPFPRITKKQMNPAVPPKPSVSDRDHLNIADDKPIPAERRRGSSHDMPLPPIPSSIQKPLPQKPPILSSKYSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP06</accession>
    <name>A0A6I9YP06_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="24">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">GALNT2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. Involved in O-linked glycosylation of APOC-III, ANGPTL3 and PLTP. It participates in the regulation of HDL-C metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 24">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="24">Golgi apparatus membrane</location>
            <topology evidence="24">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="24"/>
    <dbReference type="RefSeq" id="XP_013925649.1">
        <property type="nucleotide sequence ID" value="XM_014070174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP06"/>
    <dbReference type="GeneID" id="106551994"/>
    <dbReference type="KEGG" id="tsr:106551994"/>
    <dbReference type="CTD" id="2590"/>
    <dbReference type="OrthoDB" id="429263at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23434">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.460:FF:000001">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000018">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000026">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF49">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 24" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16 24" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 24" id="KW-0430">Lectin</keyword>
    <keyword evidence="20 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="26" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026971011" description="Polypeptide N-acetylgalactosaminyltransferase" evidence="26">
        <location>
            <begin position="21"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="27">
        <location>
            <begin position="435"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00058945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013925649.1"/>
        </source>
    </evidence>
    <sequence length="565" mass="64471" checksum="8F52A1F5CEF3A65C" modified="2020-10-07" version="1">MRRRSRLLLCFAFLWVLGIAYYMYSGGSAALAGGGRKEDPNNINTLKKKDQHSNGDEKSQNLETLPPGKVRWQDFDQDGYVGVTMVKAGQDPYARNKFNQVESDKLRMDRNVQDTRHDQCQRKQWRIDLPATSVIITFHNEARSALLRTVVSVLKKSPPHLIKEIILVDDYSNDSEDGALLGKIEKVKILRNDRREGLMRSRVRGADAAQAKVLTFLDSHCECNEHWLEPLLERVAEDKTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRARQGNPVASIKTPMIAGGLFVMDKLYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRMELRKRLNCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKDKSVKHMDLCLTVVDRTASSLIKLQGCRENDSRQKWEQIESNTKLRYVGNNLCLDSRNAKNGGLTVEVCSPSLSQQWKFTLNLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ60</accession>
    <name>A0A6I9YQ60_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pro-interleukin-16-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926427.1">
        <property type="nucleotide sequence ID" value="XM_014070952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ60"/>
    <dbReference type="GeneID" id="106552631"/>
    <dbReference type="KEGG" id="tsr:106552631"/>
    <dbReference type="OrthoDB" id="42382at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042609">
        <property type="term" value="F:CD4 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050930">
        <property type="term" value="P:induction of positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030595">
        <property type="term" value="P:leukocyte chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055287">
        <property type="entry name" value="IL-16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48484">
        <property type="entry name" value="PRO-INTERLEUKIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48484:SF2">
        <property type="entry name" value="PRO-INTERLEUKIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926427.1"/>
        </source>
    </evidence>
    <sequence length="716" mass="78578" checksum="D6CFECFA347B39B1" modified="2020-10-07" version="1">MNKKIKDKKRTIPLARADILLTLWCVLGKADSSARPALRRQARVDYSLEATPEDPWVRISDCIKSLFNPSMAEERCSLNMSHGVESKDGEATLHCSDVALQPSDTEESDPKASSTSEEGDCVKKGPPVAPKPAWFRQSLKGLKKGNSDVKPLRNSISEHQSMSDNEAGSMTKITRASPRGSSIKQRISSFENLGTPQSPEKGTRKLSPKPSVRKEEASSRRDSQMAKFDPQEASTSNIDHRGSEESSHRPSPQGEDQIGSSSSSSVEHCASISKTSSSPRLDSSLNLPASQNTELDPPVTKAPSQRARSFPLTSSQTCEVLKNKAEGEKCSKIYSISNHFSSALMKSLDGFPSRSPRCPRKSPGTSNGGISESCPEEDKVAVPQGREVQSLDTGYSLNEISKEIKCELEEVGDLLRLRNKTVVTHGTFASSLAPRAAEQQKVSSGAADREKKNSQGGSWLKKSPCEKYLEKNDAHLNHPSQKPMIRSSSDSSYNPRSSCGNGLLYQLTNGQGKEHHLDVAITQPPGSRHSFSEMSLRNESSPSASQGARPLSPHTTKKCTEILVRKPRSFKPKPPPRKYFKQDCPDNEQMIREMKERPNLQESEVPPSSNTQVRQASGGQKELKNANNGEKLLRKNSCYPSMHQGQVVSSRSMTPQHNPPVQPGDELLQVHTYLMQGHTRFEAWNIIRTLPDGFITAIIRRKNSGAGTAPSLHGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQN2</accession>
    <name>A0A6I9YQN2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="5 13">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106552949</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="5 13"/>
    <dbReference type="RefSeq" id="XP_013926833.1">
        <property type="nucleotide sequence ID" value="XM_014071358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQN2"/>
    <dbReference type="GeneID" id="106552949"/>
    <dbReference type="KEGG" id="tsr:106552949"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000019">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926833.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="18961" checksum="F3ABD8FC8DF4EC77" modified="2020-10-07" version="1">MSLSHKLTLDKVDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKHCLDNGAKSVVLMSHLGRPDGVPMPDKFSLEPVVAELKSLLGRDIIFLKDCVGSEVESACASPADGSVILLENLRFHVEEEGKGKDLSGNKIKADSAKVDAFRASLSKLGDVYINDAFGTAHRAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQY3</accession>
    <name>A0A6I9YQY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Receptor-type tyrosine-protein phosphatase S</fullName>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="37" type="primary">PTPRD</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="37">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Synapse</location>
            <location evidence="26">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013926712.1">
        <property type="nucleotide sequence ID" value="XM_014071237.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106552856"/>
    <dbReference type="KEGG" id="tsr:106552856"/>
    <dbReference type="CTD" id="5789"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd05738">
        <property type="entry name" value="IgI_2_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05739">
        <property type="entry name" value="IgI_3_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14628">
        <property type="entry name" value="R-PTP-D-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14624">
        <property type="entry name" value="R-PTPc-D-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000027">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000036">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000066">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000068">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000144">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000015">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000082">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000128">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000002">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000098">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase F isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045905">
        <property type="entry name" value="R-PTP-delta_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF11">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0966">Cell projection</keyword>
    <keyword evidence="25" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="24" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="19 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="21 37" id="KW-0675">Receptor</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="11 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 31" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="1139"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="34">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="34">
        <location>
            <begin position="107"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="196"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="291"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="389"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="483"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="585"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="694"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="792"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="892"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="32">
        <location>
            <begin position="1275"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="33">
        <location>
            <begin position="1450"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="32">
        <location>
            <begin position="1562"/>
            <end position="1821"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="33">
        <location>
            <begin position="1739"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="465"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1169"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="465"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="1169"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="1188"/>
            <end position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00073611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="RefSeq" id="XP_013926712.1"/>
        </source>
    </evidence>
    <sequence length="1830" mass="205414" checksum="3E4927CF955C0B29" modified="2020-10-07" version="1">MGPQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDTSNNNGRIKQLRSESIGGTPIRGALQIELSEESDQGKYECVATNSAGTRYSAPANLYVRELREVRRVPPRFSIPPTNHEIMPGGSVNITCVAVGSPMPYVKWMLGAEDLTPEDDMPIGRNVLELNDVRQSANYTCVAMSTLGVIEAIAQINVKALPKPPGTPVVTESTATSITLTWDSGNPEPVSYYIIQHKPKNSEELYKEIDGVATTRYSVAGLSPYSEYEFRVVAVNNIGRGPPSEPVTTRTSEQAPSSAPRNVQARMLSSTTILVQWEEPEEPNGQIQGYRVYYTMDPSQHVNSWLKHNVADSHITTIGNLVPQKTYSVKVLAFTSVGDGPLSSDIQVITQTGVPGQPLNFKAEPESETSILLSWTPPRSDTISNYDLVYKDGEHGEEQRISIDPATSYHLQGLKPNSLYYFRLAARSPQGLGASTTEISARTMQSKPSAPPQDISCNSPSSTSILVSWQPPPMEKQNGIITSYSIKYTGTDGEDDKPHEILGITPDTTHYLLEQLEKWTEYRISVAAHTDVGPGPESESVFIRTDEDVPSGPPRKVEVEAVNSTSVKVSWRSPVPNKQHGQIRGYQVHYVKMENGEPRGQPMLKDIMLADAQWEYDDTTEHEMIIAGLQPETTYSLTVTAYTTKGDGARSKPRLVSTTGAVPGKPRLVISHTQMGTALIQWHPPVDTYGPLQGYRLRFGRKDMDAFMTRDFTDKDDHYTATDIHKGTSYIFRLSARNKVGFGEEMVKEILIPEDLPSGFPQNLHSESATSTSVQLTWQPPILSERNGIITKYTLQYRDINVVNQQTEIPVAPVDTIMTLSGLKPDTTYDVKIRAHTSKGPGPYSPSIQFRTLPVDQAVFAKNFHVKAVMKTSVLLSWDIPENYNSALPFKILYDDGKMAVEVDGRATQKLIINLKPETSYSFVLTNRGNSAGGLQHRVTAKTAPDVLRTQPLFIDKTNLDGMITVKLPEVPANKTIKGYYIVIVPLIKKSDGKFMKPRESPDEMELDELLKEIARKRRSIRLGREADLKPYIAAHFEVLPTEFTLGDKKQYGGFENKQLQNGQEYVVFILAVMEHSESTMYATSPYSETVVSVDLNPQPITEEEEGLIWVVGPVLAVVFIICIVIAILLYKSSKPDRKRADTDSRKNSIPNSKEIPSHHPTDPVELRRLNFQTPALSSNSVPYASLIGSVSSLSSQTTTQSLYDNNSLPRFARKGMASHPPIPILELADHIERLKANDNLKFSQEYESIDPGQQFTWEHSNLEVNKPKNRYANVIAYDHSRVLLSAIEGIPGSDYINANYIDGYRKQNAYIATQGALPETFGDFWRMMWEQRSATVVMMTKLEERSRVKCDQYWPSRGTETYGLIQVTLLDTVELATYCVRTFALYKNGSSEKREVRQFQFTAWPDHGVPEHPTPFLAFLRRVKTCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERIKHEKTVDIYGHVTLMRAQRNYMVQTEDQYIFIHDALLEAVTCGNTEVPARNLYAYIQKLTQIETGENVTGMELEFKRLASSKAHTSRFISANLPCNKFKNRLVNIMPYESTRICLQPIRGVEGSDYINASFIDGYRQQKAYVATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTDWPEQGVPKSGEGFIDFIGQVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVKMLRTQRPAMVQTEDQYQFCYRAALEYLGSFDHYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRR2</accession>
    <name>A0A6I9YRR2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat and SOCS box protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ASB6</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926619.1">
        <property type="nucleotide sequence ID" value="XM_014071144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRR2"/>
    <dbReference type="GeneID" id="106552787"/>
    <dbReference type="KEGG" id="tsr:106552787"/>
    <dbReference type="CTD" id="140459"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03725">
        <property type="entry name" value="SOCS_ASB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037327">
        <property type="entry name" value="ASB6_SOCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24132">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24132:SF24">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="3">
        <location>
            <begin position="363"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013926619.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="47539" checksum="36DE75A31B809CB6" modified="2020-10-07" version="1">MPFLHGFRRIIFEYQPLVDAILESLGLQDPEHQELPESCRSAAEDHSKFLVLQKLLESESHSAFYQEGVSYTLLKVSELGLVRAAEVLLDNGADITFEDPVTYYTALHIAVLRNQPDMVELLVQRGADINRRDRIHESSPLDLASEEPERLPCLRRLLELGANINAADKHGKTALLHALASSDGVQIHNIENIRLLLEGGADVKATTKDGDTVFTFIIFLLGETVGSDAEEAKMINRFCFQATRLLMAHGANPSECPPYESLTHLCLKSFRLHFPLLRFLLESGASHSCSLHGPTCWSGFDIIFERLCSHLSESEDDSFSIDLLQKAETALELMIASGPVVKLPSNFQIDLSHCRYHQEKIQALFGKLKQLESTPPTLKHLCRVYLRKFLQPWPIDVKVKALPLPNRLKRYLLIDPSASLEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS53</accession>
    <name>A0A6I9YS53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Receptor-type tyrosine-protein kinase FLT3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106552895</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926764.1">
        <property type="nucleotide sequence ID" value="XM_014071289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS53"/>
    <dbReference type="GeneID" id="106552895"/>
    <dbReference type="KEGG" id="tsr:106552895"/>
    <dbReference type="OrthoDB" id="6077854at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030183">
        <property type="term" value="P:B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001927">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF356">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN KINASE FLT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013926764.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17998" checksum="6D170340C53ABECB" modified="2020-10-07" version="1">MNDSNYVVRGNARLPVKWMSPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGMQVNEKFYKLIQNGFKMDRPFYATEEIYLLLCSCWALDSRKRPSFSQLLSLLACQLAEAEGTACRTDRTDASKRVSSSKCNPPVRREAELATPLLNENIARGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSG5</accession>
    <name>A0A6I9YSG5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cyclin-dependent kinase 12</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cell division protein kinase 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC106552752</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013926580.1">
        <property type="nucleotide sequence ID" value="XM_014071105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSG5"/>
    <dbReference type="GeneID" id="106552752"/>
    <dbReference type="KEGG" id="tsr:106552752"/>
    <dbReference type="OrthoDB" id="28397at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000074">
        <property type="entry name" value="cyclin-dependent kinase 12 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF126">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="204"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="135"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013926580.1"/>
        </source>
    </evidence>
    <sequence length="347" mass="40214" checksum="EDABCE5C04C03C52" modified="2020-10-07" version="1">MKEAQLRLSWAEEHLLSIRAEHIAGTDNVTADWLSRSQVDHAEWELHPTHFWELTRRFGAPVVDLFTTTNNKKVPRFYTRFPKMLNGLGAEQMADAILRSNLERKAIIFVSHNTFLWCFSPKEILPLKSVKKEKEQKRPRHLLTDLPLPPELPGGDPSPPESPEPKSVTPLQQSLRKRPKICCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHQSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLDYCHKKNFLHRDIKCSNILLNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU39</accession>
    <name>A0A6I9YU39_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">IQ domain-containing protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">IQCE</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927556.1">
        <property type="nucleotide sequence ID" value="XM_014072081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU39"/>
    <dbReference type="GeneID" id="106553554"/>
    <dbReference type="KEGG" id="tsr:106553554"/>
    <dbReference type="CTD" id="23288"/>
    <dbReference type="OrthoDB" id="2136082at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052318">
        <property type="entry name" value="CellDiv_DevSignal_Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22590:SF3">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22590">
        <property type="entry name" value="MYOSIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013927556.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="28349" checksum="F7706E4C5000ADE0" modified="2020-10-07" version="1">MSHSKSDIGIRTASSPLAGSTPEYLKEALGMKKPKHARSASRGYIPGTPDYKEKEDMYDEIIELKKTIQAQKCEADRMKTKLRRVEEDNTRKDRQMEQLLEPFKYSEFSWVRTEQKNDSSLMINGLKQKILRLEQQCKEKDNTINQLQADMKNTNMEEMTVALHTSYQEIRRLQNLLTRSKVVQRKASESPRQKLLSTAVFQLSERMKELQEQNERLKAEMDHALSSFLSSSQAKNYLARQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU47</accession>
    <name>A0A6I9YU47_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mis18-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106553515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927509.1">
        <property type="nucleotide sequence ID" value="XM_014072034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU47"/>
    <dbReference type="GeneID" id="106553515"/>
    <dbReference type="KEGG" id="tsr:106553515"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013927509.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="32331" checksum="DC425E33DE1E78C0" modified="2020-10-07" version="1">MSLKAVSLQSIPGGTPLKDFVKFQGNCTLKSAKIPPSMVLSCHNVCSGQETKGIEVFQSTLISSNAHIQEFFDISEIKASEGMLGRISSCQKTKHMSRSAVQKRKACEPLSHESPAKIFSRMKQKAALVKEKNKPPEAKLLDTKHTADYTPERQPAFLPSSEKIPIVEDKQKTKVDEDFLEESHVKTVSVTDFTAVNKTTCTTVISPLLLESPNKFFSRFSKKKDVIYLNEWGIRVINDNTAVCLEGIRSMSLSLTSGIHTIRVLQQIQVAHEVTYSRFLYLIIGKDKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUD9</accession>
    <name>A0A6I9YUD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DHX15</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013927922.1">
        <property type="nucleotide sequence ID" value="XM_014072447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUD9"/>
    <dbReference type="GeneID" id="106553867"/>
    <dbReference type="KEGG" id="tsr:106553867"/>
    <dbReference type="CTD" id="1665"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF95">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013927922.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="47459" checksum="D04D60A937FCFF6F" modified="2020-10-07" version="1">MVCENNLQGLSPKTLSDKRQHRLQLLEIDEACKRIKREIDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKPSGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEILSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVTSSKIKGREVSGLVKIAPQYYDMSNFPQCEAKRQLDRIIVKLQSKEYSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUG0</accession>
    <name>A0A6I9YUG0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 35 member E4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC35E4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Putative transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TPT transporter family. SLC35E subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927942.1">
        <property type="nucleotide sequence ID" value="XM_014072467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUG0"/>
    <dbReference type="GeneID" id="106553884"/>
    <dbReference type="KEGG" id="tsr:106553884"/>
    <dbReference type="CTD" id="339665"/>
    <dbReference type="OrthoDB" id="10261634at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="8">
        <location>
            <begin position="49"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927942.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="39069" checksum="7882B50A9124AA63" modified="2020-10-07" version="1">MCLPAGKKHDAVMGSATGAWLLPPWKQDPGQRWGRRLLSRWGHSLPLVATVVTWLATGSTMSGLNKWIFATHNFRYPVLLSSLHMLTAVVVGRPLARLRDRGGGGGGGGSGYPSLHPKTRLRVFLLSLTFCASVAFGNLGLNHVQLDFAQMVYTTTPLFTLTLSELLLRKRHHPLQYAAMGPICLGAALSFVGEVHFDQAGCCFLFAATFLRGLKSIQQSILLQEEQLDSLRLLCLTSLPSFCLLFVAALGMEVGSAWEGLLTYEASLWNCVLLSCLGSVLYNLSSFCILSLTSALTIHVLGNFNVVGNLLLSRWLFGSHLTWLSYVGIALTLLGVLVYHHSEGIAACWRSLAGPAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV00</accession>
    <name>A0A6I9YV00_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106553793</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106553793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927836.1">
        <property type="nucleotide sequence ID" value="XM_014072361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV00"/>
    <dbReference type="GeneID" id="106553793"/>
    <dbReference type="KEGG" id="tsr:106553793"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="92"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927836.1"/>
        </source>
    </evidence>
    <sequence length="365" mass="41558" checksum="F6A51EC3BF7465EB" modified="2020-10-07" version="1">MFWQVMQEEDGDADSLEGLLVPKPDQSSNPEEEEEQVFIQFPVESERITDHETGPKDSSLSEAAPSPSGCARFSRNISCPLRDFAGSHWKHPRWTEDEIQAFIEIWGDTSVQRALFSNYRTVSQFEWISCQMKARGYDRNWLQCREKAKHLRKSYKDCLGGHSHWGSGRRTWPFFKQLNQFLRVEQELVVPREIGRQEVQHHLVDRRRKRMKNSAGPVAKAAQVQVEVEAHELQTKKQAKHPPRFPPNGTSRSGNQKPPLTSIERIRNIHKSRQERRADVGQARSGIGDSAAGSPLTDPPLPGSHKADPAPSSEIEKAWEECERKAVEKLEGYLRNKGWKLQTGHSSRTGILQDVLSIVCKGRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV45</accession>
    <name>A0A6I9YV45_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lipoma HMGIC fusion partner-like 4 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LHFPL4</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928478.1">
        <property type="nucleotide sequence ID" value="XM_014073003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV45"/>
    <dbReference type="GeneID" id="106554354"/>
    <dbReference type="KEGG" id="tsr:106554354"/>
    <dbReference type="CTD" id="375323"/>
    <dbReference type="OrthoDB" id="5873721at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060077">
        <property type="term" value="C:inhibitory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050811">
        <property type="term" value="F:GABA receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097112">
        <property type="term" value="P:gamma-aminobutyric acid receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019372">
        <property type="entry name" value="LHFPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489:SF14">
        <property type="entry name" value="LHFPL TETRASPAN SUBFAMILY MEMBER 4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489">
        <property type="entry name" value="LIPOMA HMGIC FUSION PARTNER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10242">
        <property type="entry name" value="L_HMGIC_fpl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928478.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="16973" checksum="CD5A1F136A807804" modified="2020-10-07" version="1">MLPSQEASKLYHDNYMRNSRAIGVLWAIFTICFAIINVVVFIQPYWIGDSVSTPKPGYFGLFHYCVGNEGNNRELTCQGSFADFRSIPSGAFKAASFFVLLSMVLILGCITCFALFFFCNTATVYKICAWMQLLAVCIGGGDPLRPACNEISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWD3</accession>
    <name>A0A6I9YWD3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">SERTA domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">SERTAD4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5 6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928607.1">
        <property type="nucleotide sequence ID" value="XM_014073132.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928614.1">
        <property type="nucleotide sequence ID" value="XM_014073139.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928622.1">
        <property type="nucleotide sequence ID" value="XM_014073147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWD3"/>
    <dbReference type="GeneID" id="106554463"/>
    <dbReference type="KEGG" id="tsr:106554463"/>
    <dbReference type="CTD" id="56256"/>
    <dbReference type="OrthoDB" id="5976204at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009263">
        <property type="entry name" value="SERTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029708">
        <property type="entry name" value="SERTAD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14272">
        <property type="entry name" value="SERTA DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14272:SF4">
        <property type="entry name" value="SERTA DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06031">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51053">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERTA" evidence="2">
        <location>
            <begin position="128"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013928622.1"/>
        </source>
    </evidence>
    <sequence length="363" mass="40489" checksum="ACF3090E0517B323" modified="2020-10-07" version="1">MTLVLPLSSFCEPIVSSEGAGEFQPLWEARCCSKSSPALDSAAAGSSNSNSGGSSSSSTAQGQAQQQPPPQGSDGRTPVGQPPPSALHQRGNPNPGASSKITYVKRKYVEEEDFHPPLSNCTPKTISVFEERAHILYMSLEKLKYIDDPEVYLRRSVLINNLMKRIHGEIIMQNNWCFPTCSFGGTSSQEWFMSHDCPYRKRLRMAREDCEKIHTCCFYQECGSHYLSLPLSTNSAVGNSTASVSLPSCSQQVEYGSNSSPVYRSDEHIHANEIFITNAGPHDNQDKSIFSNEKGGHDIDREHASDWESMKSDHVIECKNKYYNYFEMGHDDKSNMNECWKKCLRKRECLPGSKICCSKGNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWH0</accession>
    <name>A0A6I9YWH0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">TRIM41</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Neurotoxin that produces dose-dependent hypolocomotion and hyperalgesia in mice. May directly act on the central nervous system, as it is 6500-fold more potent when administered intracerebroventricularly than intraperitoneal.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 27">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ohanin/vespryn family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013928336.1">
        <property type="nucleotide sequence ID" value="XM_014072861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWH0"/>
    <dbReference type="GeneID" id="106554236"/>
    <dbReference type="KEGG" id="tsr:106554236"/>
    <dbReference type="CTD" id="90933"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19762">
        <property type="entry name" value="Bbox2_TRIM7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000025">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM41 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0528">Neurotoxin</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0800">Toxin</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="23">
        <location>
            <begin position="257"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="24">
        <location>
            <begin position="448"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="57"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="73"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013928336.1"/>
        </source>
    </evidence>
    <sequence length="638" mass="72449" checksum="52DEBFADB04B9958" modified="2020-10-07" version="1">MAAAVAAGTSGAPLNAVATLHEEAVCAICLDYFIDPVSIGCGHNFCRVCITQLWGAGGEEEGDDVGASGSERADEDDDDDRGIDDLGPEEDGDYDDDENDLEDNDYLDDGDMGDEEDDDDDVSRDGYDDEDEDAWEDDDRDYWEQDIWDSTMGDDLFFDNYDEDEEVMDEEPDEMAEYAVPATPPTPQRQSFTCPQCRKTFPHRNFRPNLQLANMVQIIRQMHPQPPKPANTSADVVEMEAAGDSSGVPSILAASHAPRALCEKHQEPLKLFCEADEEPICVVCRESRTHKHHSVMPLEEVVQEYKAKIQGHLDPLKKKLESVLKQKSSEEEKITDIKNKMKLDMKEFESDFERLHQFLVGEQATLLHQLEDRYEILLGSQSSNISHLEEQGAALSRLIAEAEDKSKQDGLQLLKDFKGTLVRCENITFQDPEMLPVDTGKKYRNYFLIDVLMKKMEKVFSKAPQVDLTLDPETAHPRLTISSDCRSVRLGERWRDLPDNPKRFDSDYCVLALQGFTYGRHYWEVEVGGRRGWAVGAARESARRKEKSSSGSHQKREIWCVGTNGKKYQALTSTEQTSLSPAEKLRRFCVYLDYERGQLGFYNAENMAHIHTFNASFHERIFPFFRILSKGTRIKICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWT4</accession>
    <name>A0A6I9YWT4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Potassium voltage-gated channel subfamily H member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KCNH1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928489.1">
        <property type="nucleotide sequence ID" value="XM_014073014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWT4"/>
    <dbReference type="GeneID" id="106554362"/>
    <dbReference type="KEGG" id="tsr:106554362"/>
    <dbReference type="CTD" id="3756"/>
    <dbReference type="OrthoDB" id="447251at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000442">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF530">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY H MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="10"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928489.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="40203" checksum="52291300B0831103" modified="2020-10-07" version="1">MAFVSSDLQSKGDVFGDVFWKETTLAQSCANVRALTYCDLHVIKRDALQKVLEFYTAFSHSFSRNLILTYNLRKRIVFRKISDVKREEEERMKRKNEAPLVLPPDHPVRRLFQRFRQQKEARLAAERGGREDLDVERGNILGDHTSHTVVKASIVTVRESPATPVAYQSASTSGIGISDQAKLQAPVSDYVGGKVAGDFPKRKGWAKFKDACAKGEDWNKVSKAESMETLPERTKATGEATLKKTDSCDSGITKSDLRLDNVGETRSPQDRSPILAEVKHSFYPIPEQTLQATMLEVKHELKEDIKALNTKMTNIEKQLADILRILASRRSSQTPQELYEISRPQSPESEKDMFGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWT7</accession>
    <name>A0A6I9YWT7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LON peptidase N-terminal domain and RING finger protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554312</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928436.1">
        <property type="nucleotide sequence ID" value="XM_014072961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWT7"/>
    <dbReference type="GeneID" id="106554312"/>
    <dbReference type="KEGG" id="tsr:106554312"/>
    <dbReference type="OrthoDB" id="264917at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.40:FF:000005">
        <property type="entry name" value="LON peptidase N-terminal domain and ring finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF4">
        <property type="entry name" value="LON PEPTIDASE N-TERMINAL DOMAIN AND RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Lon N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928436.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="24860" checksum="01CD87E2E5822AA9" modified="2020-10-07" version="1">MYLTRNVPIFVCTLAYPTVPCPLHVFEPRYRLMIRRSIETGTKQFGMCVQDPQNGFADYGCMLFIRNLDYLPDGRSVVDTIGLKRFRVIRRGMKDGYCTADIEDLEDIKVKDEGEMRKLQELHNIVYNQACSWFQSLRNKFHSQILQYFGPMPEKEENLQETADGPAWCWWLLAVLPVDPKYQLSVLSMKSLRERLIKIQAILTYFSRDQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYT3</accession>
    <name>A0A6I9YYT3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106554975</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929204.1">
        <property type="nucleotide sequence ID" value="XM_014073729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYT3"/>
    <dbReference type="GeneID" id="106554975"/>
    <dbReference type="KEGG" id="tsr:106554975"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="294"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929204.1"/>
        </source>
    </evidence>
    <sequence length="560" mass="63533" checksum="A10F100D40058C3A" modified="2020-10-07" version="1">MAYKTQAIKCSFQDPIPIVHKYFQPGDLIIGGITKFVFTTTDAESFKEDPHHTLVSETLVLSKYYQHNLAMIFAVKKINEDIQILPNITLSYNIYDRYEDADFQAKLEIFSSQNKLFPNYKCGILNKAISVIGNIDQGMFYMDILDTYKVPQEKYEWQKIQSSGSPAMAGLDIENWITFPNQSFHQLKVGKLDPWAAEDKMFSIHEEALTWPSTFNQTLPVSLCTENCLRGYFRRKQEEKPFCCYDCLPCPKGKMSDQEDMDDCLKCAEDQYPNKNQNSCLLKTTSFLSYEEPLGISLVTLVLFFSLCTLAVLATFLKYHHTPIVKANNRNLSYVLLISLLLCFLCSLQFLVVPGNIICLLRQVSFGIIFSVAVSSVLAKTITVVLAFMASKPGSRMRKWVGRGLATSIVLSCSLIQAVICMVWLFTFPPFPDIDTLSEIEEMILQCNEGSDIMFYCVLGYMGLLATASFTVAFFSRNLPSSFNETKCLTFSMLIFCSVWLSFVPSYLSTKGKYMVAVEVFSILASSAGLLVCIYFPKCYIIIFRPELNSRKQLIKRNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0F6</accession>
    <name>A0A6I9Z0F6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Chloride intracellular channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">CLIC1</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="33">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="28">In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Reduces selenite and dehydroascorbate and may act as an antioxidant during oxidative stress response. Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Involved in regulation of the cell cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate</text>
            <dbReference type="Rhea" id="RHEA:24424"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.8.5.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:24425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>bromide(in) = bromide(out)</text>
            <dbReference type="Rhea" id="RHEA:75383"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>iodide(out) = iodide(in)</text>
            <dbReference type="Rhea" id="RHEA:66324"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>nitrate(in) = nitrate(out)</text>
            <dbReference type="Rhea" id="RHEA:34923"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>thiocyanate(in) = thiocyanate(out)</text>
            <dbReference type="Rhea" id="RHEA:75347"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Membrane</location>
            <topology evidence="30">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="30">Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 30">Belongs to the chloride channel CLIC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930473.1">
        <property type="nucleotide sequence ID" value="XM_014074998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0F6"/>
    <dbReference type="GeneID" id="106556040"/>
    <dbReference type="KEGG" id="tsr:106556040"/>
    <dbReference type="CTD" id="1192"/>
    <dbReference type="OrthoDB" id="1935530at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd10300">
        <property type="entry name" value="GST_C_CLIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03061">
        <property type="entry name" value="GST_N_CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000001">
        <property type="entry name" value="Chloride intracellular channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000129">
        <property type="entry name" value="Chloride intracellular channel protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002946">
        <property type="entry name" value="CLIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030259">
        <property type="entry name" value="CLIC-1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053823">
        <property type="entry name" value="CLIC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00862">
        <property type="entry name" value="O-ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45476:SF2">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45476">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22441">
        <property type="entry name" value="CLIC-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01263">
        <property type="entry name" value="INTCLCHANNEL"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19 30" id="KW-0868">Chloride</keyword>
    <keyword evidence="18 30" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="7 30" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="21 30" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15 30" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 30" id="KW-0813">Transport</keyword>
    <keyword evidence="11 30" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="GST C-terminal" evidence="31">
        <location>
            <begin position="70"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00024145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00035073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00035085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00036895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00044702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00046117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="RuleBase" id="RU362009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_013930473.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="27169" checksum="616D8DD772467F7D" modified="2020-10-07" version="1">MAEEKPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTEAVQKLCPGGQLPFLMYGTEVHTDTNKVEEFLEEVLCPPKYPKLAANNPESNTAGLDVFAKFSAYIKNSNPSHNANLEKGLLKALKVLDNYLMTPQPEEVDENSAEDEGQSNRKFLDGNELTLADCNLLPKLHIVKVVCKKYRDFTIPEEFRGIHRYLKNAYAREEFSSTCPDDEEIELAYELVAKALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0N2</accession>
    <name>A0A6I9Z0N2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase lipid-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATPase protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ATPase subunit c</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">ATP5G1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929420.1">
        <property type="nucleotide sequence ID" value="XM_014073945.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013929421.1">
        <property type="nucleotide sequence ID" value="XM_014073946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0N2"/>
    <dbReference type="GeneID" id="106555166"/>
    <dbReference type="KEGG" id="tsr:106555166"/>
    <dbReference type="CTD" id="516"/>
    <dbReference type="OrthoDB" id="438052at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18182">
        <property type="entry name" value="ATP-synt_Fo_c_ATP5G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.20.10:FF:000003">
        <property type="entry name" value="Atp synthase f complex subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031:SF54">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT C2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="ATP SYNTHASE LIPID-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0138">CF(0)</keyword>
    <keyword evidence="7 16" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="17">
        <location>
            <begin position="69"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013929420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013929421.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="14211" checksum="CC83913D46535E38" modified="2020-10-07" version="1">MQTPVTLLASPALLRCCSRILTRPASLSLLSRPETQTVQPFGFSTHQLAHREIQTSSVSRDIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMVAFLILFAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0P4</accession>
    <name>A0A6I9Z0P4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Plasma protease C1 inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SERPING1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930568.1">
        <property type="nucleotide sequence ID" value="XM_014075093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0P4"/>
    <dbReference type="GeneID" id="106556122"/>
    <dbReference type="KEGG" id="tsr:106556122"/>
    <dbReference type="CTD" id="710"/>
    <dbReference type="OrthoDB" id="6433428at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF159">
        <property type="entry name" value="PLASMA PROTEASE C1 INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026940212" evidence="3">
        <location>
            <begin position="24"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="179"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930568.1"/>
        </source>
    </evidence>
    <sequence length="527" mass="59779" checksum="447A5E328A571945" modified="2020-10-07" version="1">MKTWLILLCLAIMTISSLQQVASRRETVAERTEELKVVKCQDETRFQKFWSWLWRRKKTPCIEEDNEITPTPFQDVERREEPALNADTGTNYENNLDYQETLVKTTQGPLHKENGEEKESAKVPTERPTTENTSPIPTVSDTSCPSNSCSQRWKPWPTCAQSTPEDEQKLAEALSRFSLEFYKIAIQKNDGNMVFSPLSIITTLSNLLLGSCGETKDRLEDLLFYPKEFACVHRALKALGKSEALTSANAIFYQPKFKMASGFSNLTSTFYQTKLKLLSNNTQQAVTDINSWVSERTGNKIKKLLDDLDSDTQMVLLNAVYFHSKWKTIFKKKNTITEDFYRPDLPSIKVPMMTSKKYPVTSYFDYNLQAKVGRFQLHDDLSLVILVPRSQFINLSEIEERLTATTVMAALDRLEALHVKPTIVMLPKFKLESSQDLAEIIGEMDFGLFYDADLCGMTQGELIALSSAKHKSVLQVSEEGVEGAAATAVNLARTATVFEVQQPFIFLFVRDRRVPVFMGRVTNPLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z117</accession>
    <name>A0A6I9Z117_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Voltage-dependent N-type calcium channel subunit alpha-1B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106556239</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930703.1">
        <property type="nucleotide sequence ID" value="XM_014075228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z117"/>
    <dbReference type="GeneID" id="106556239"/>
    <dbReference type="KEGG" id="tsr:106556239"/>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000063">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031649">
        <property type="entry name" value="GPHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014873">
        <property type="entry name" value="VDCC_a1su_IQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF6">
        <property type="entry name" value="VOLTAGE-DEPENDENT N-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08763">
        <property type="entry name" value="Ca_chan_IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16905">
        <property type="entry name" value="GPHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01062">
        <property type="entry name" value="Ca_chan_IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027097318" evidence="18">
        <location>
            <begin position="32"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013930703.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="41516" checksum="724F1EF174BA36F0" modified="2020-10-07" version="1">MKNSMAMRRHNNFQTFLQALMLLFRSATGEAWHEIMLACLSNQPCDEHSNLSRNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKNCPARIAYKRLVRMNMPISNDDLSVHFTSTLMALIRTALDIKLASGVLQHQCDAELRKEVSMVWPNLSSKNLDLLVPPHKPEAMTVGKVYAALMIFDFYKQNKNTRDPSHSGPGCQTGTVSLFHPLKAALEQSQTGFPNAFLRQKSSASLNNGGTLPMPEGGGIKESVSWGIQHTQNMFYETRTPAFERGHSEEIPVQQVVEMQEISPTLANGEHQPRHGSRSPLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z121</accession>
    <name>A0A6I9Z121_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Proto-oncogene Mas-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106556245</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family. Mas subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930708.1">
        <property type="nucleotide sequence ID" value="XM_014075233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z121"/>
    <dbReference type="GeneID" id="106556245"/>
    <dbReference type="KEGG" id="tsr:106556245"/>
    <dbReference type="OrthoDB" id="9043355at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000193">
        <property type="entry name" value="Mas-related G-protein coupled receptor member E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026234">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334:SF69">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02108">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="103"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013930708.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="42165" checksum="939842B92C793F57" modified="2020-10-07" version="1">MEQEFYHSADNAGGLLDGNGMWDEYSIGILEPEKIIWYIPQPNVSDPELVSTPFNTTEIVEIVSNITRIEEIYPYPYEQLSNNLTRNLSNGFIVIICILGLVGNGRTIYLLAHRIKRNPFTTFILNLSIADFGVAVSLIISAIFVAVLSLVQRTYTVRTFFFLFFELFFFTYSASQFLLTAISLDRCVAVLFPLWHRCHRPPHLSPLVCGLIWIVSFLLSAVHFILYLTNSFGKSRFLYQMIVNGLLCTPLMVVSTVTLWIHVRSKSQHNQRKLLTTILLALLFFLLLSLPMNVFYVIEYFGSYNPVLMTVGIGCTALSSSINPLLYFLMGRSKMGKDKPRASLKVALQRVFKEEQDSPEDSNTTEEYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z357</accession>
    <name>A0A6I9Z357_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cas scaffolding protein family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CASS4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CAS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930659.1">
        <property type="nucleotide sequence ID" value="XM_014075184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z357"/>
    <dbReference type="GeneID" id="106556197"/>
    <dbReference type="KEGG" id="tsr:106556197"/>
    <dbReference type="CTD" id="57091"/>
    <dbReference type="OrthoDB" id="5983572at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.830:FF:000001">
        <property type="entry name" value="BCAR1 scaffold protein, Cas family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.830">
        <property type="entry name" value="Serine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021901">
        <property type="entry name" value="CAS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037362">
        <property type="entry name" value="CAS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014928">
        <property type="entry name" value="Serine_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038319">
        <property type="entry name" value="Serine_rich_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654">
        <property type="entry name" value="CAS SCAFFOLDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654:SF19">
        <property type="entry name" value="CAS SCAFFOLDING PROTEIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12026">
        <property type="entry name" value="CAS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08824">
        <property type="entry name" value="Serine_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Serine rich protein interaction" evidence="10">
        <location>
            <begin position="259"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="CAS family C-terminal" evidence="11">
        <location>
            <begin position="429"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="462"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013930659.1"/>
        </source>
    </evidence>
    <sequence length="614" mass="69231" checksum="89A9AB4435ED2459" modified="2020-10-07" version="1">MEWCPEKVQKLYDIPSNPEKSRSCPWQDSAVEDMYVIPTPSPQPIANPAEKHYKTLPNLWKSEWIYDVPVAPDKAKLIQTSQNHFPQQHALYDIPPNRFNPATPKISPIKDKGKSVDPKLYNIPPLHRKLTCPELPLYDVPSTRDTFVQRPASNYDVPSNVLSTRIEKESQKLTHYDIPKGTPVALAQRKENNHNSGDNLYIMPLPSSTECNVDQDRLSISSENSRTSTISTLSSSSTDSFSSSILSTFPEDLKKEATMELELAIDTLTKLQHGVSSSVASLMIFVSSKWRYPEYLEENIEEIHRAIDHIKVSLGEFLKFAQTIERNAVLGSDSKLQARIKKQLNVLSDSFQILVHTREALNKCKWSLEILAVKKPQSNPDDLDQFVMVARTLPDDIKRFSSIIIANGKLLFKKSCKDKNSKDSKAGVELKMKKFGQEKQGEDNSVLISSLSKAKENDLYSTETSLSVPEDSSTHLQSSPPTMKERTLSKQDPDNRMILSSLSRLYFGAVQKAIGVFHKTLSDNRTPEIFVAKSKLIIMIGQKLVDTLCQEALEKTNRNDVLRGSSTFCGLLKSLAIATKNAAMKYPSFEALKELQNQADELSQFTQQFRDMME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z394</accession>
    <name>A0A6I9Z394_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">carnitine O-palmitoyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">LOC106556999</name>
    </gene>
    <organism evidence="13 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4,8-dimethylnonanoyl-CoA + (R)-carnitine = O-4,8-dimethylnonanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44860"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77061"/>
            <dbReference type="ChEBI" id="CHEBI:84654"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013931561.1">
        <property type="nucleotide sequence ID" value="XM_014076086.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931562.1">
        <property type="nucleotide sequence ID" value="XM_014076087.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931563.1">
        <property type="nucleotide sequence ID" value="XM_014076088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z394"/>
    <dbReference type="GeneID" id="106556999"/>
    <dbReference type="KEGG" id="tsr:106556999"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015909">
        <property type="term" value="P:long-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.20">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042572">
        <property type="entry name" value="Carn_acyl_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF69">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 1, MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="11">
        <location>
            <begin position="176"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013931562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013931563.1"/>
        </source>
    </evidence>
    <sequence length="451" mass="51975" checksum="E77429D76CE43541" modified="2020-10-07" version="1">MAEAHQAVAFQFTVTPDGVDFRLSQEALKQIYLSGLHSWKKRFIRLKNSFLTGVYPGSPSGWLAISAVTVGSTWVHVDLSRGAISRIQKHLLSSCSRYFNLRQQTLLSALIFSTGAWVLGIWLRRQMLKVLLSYHGWMFEPHGKTSFSTKVWVALVKVMSGRHPLLYSFQTSLPKLPVPRVEDTIQRYLESVQPLLEKEKFEQMEVLARDFQQKVGTRLQKFLVLKSWWATNYVSDWWEEYIYLRGRGPIMVNSNYYLMDFLYITPTPIQAARAGNCVHAILKYRRQLDREELAPVMALGVVPMCSYQMERMFNTTRIPGKEADSLLHLADSRHLAVFHRGRFFKVWLYHGGKHLPPRDLEMQFQRILDDPSPPQPGELRLAALTAGSRVPWAEARAKFFSRGKNRASLDCIDRAAFFLALDEEPQGFSPEREESLSDYAKSLLHGHCYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5H4</accession>
    <name>A0A6I9Z5H4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RING finger protein 121-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106556743</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931201.1">
        <property type="nucleotide sequence ID" value="XM_014075726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5H4"/>
    <dbReference type="GeneID" id="106556743"/>
    <dbReference type="KEGG" id="tsr:106556743"/>
    <dbReference type="OrthoDB" id="446635at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040176">
        <property type="entry name" value="RNF121/RNF175"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13407:SF1">
        <property type="entry name" value="E3 UBIQUITIN LIGASE RNF121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13407">
        <property type="entry name" value="RNF121 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013931201.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="29283" checksum="7AA2A19A20CC543A" modified="2020-10-07" version="1">MHAKHRGHEAMHAEMVLILIATLVVAQLLLVQWKQRHPRSYNMVTLFQMWVVPLYFTVKLHWWRFLGIWVFFSAVTAFVTFRATRKPLVQTTPRLVYKWFLLIYKISYAIGIVGYMAVMFTLFGLNLLFRIKPEDGMDFGISLLFYGLYYGVLERDFAEMCADYMASTIGFYSACGMPTKHLSESVCAVCGQPIFVDVNEEGIIENTYRLSCNHVYPSTGHTRLATDSHLRRSLAPGPPIAPNTQQLTQQDNLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6B3</accession>
    <name>A0A6I9Z6B3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA-binding motif, single-stranded-interacting protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931466.1">
        <property type="nucleotide sequence ID" value="XM_014075991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6B3"/>
    <dbReference type="GeneID" id="106556941"/>
    <dbReference type="KEGG" id="tsr:106556941"/>
    <dbReference type="OrthoDB" id="9398244at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931466.1"/>
        </source>
    </evidence>
    <sequence length="87" mass="8823" checksum="8FE4BD8B8D18CB16" modified="2020-10-07" version="1">MLLSVPPRAGIATFGYNKSNKKPYVSVAQQMAPPSPSGSCNNNSSSSSSSGPGGGGGGGDQLSKTNLYIRGLHPGTTDQDLVKLCQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z890</accession>
    <name>A0A6I9Z890_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106557308</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106557308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013932000.1">
        <property type="nucleotide sequence ID" value="XM_014076525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z890"/>
    <dbReference type="GeneID" id="106557308"/>
    <dbReference type="KEGG" id="tsr:106557308"/>
    <dbReference type="OrthoDB" id="9050373at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.250.20">
        <property type="entry name" value="L1 transposable element, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042566">
        <property type="entry name" value="L1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013932000.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="37667" checksum="A35714CB514AAE32" modified="2020-10-07" version="1">MAPKSKLGRRPFSNTSQAFATKLQPLSSTPPTGDLLTKEFLFETLKEFREEMKQLITDLCDKLDTKIAQTKEDMVGLVTVLTDYVAGMEDKLELLEEANTNLTSKIEVLQQKVENAEKQLVMTNYKKMELALRVRGLRENDQENLKLTFSEALNRLVGSPGLNFDWQIQKIYRHNSWTAKQKQLPRDVVIYFATKESRNAILQELYNNRLRIDGQDLIVFKEMPPQMLRARRDYVFLTKELRNNQIQYRWEVPFGITVTLDDQKHRLNSPCEARDFYNKILKAGPLELPGLVQRQGEVEERQPITQLQGGRCCFSPPEGRRNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WVU4</accession>
    <name>A0A6I9WVU4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fas-binding factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106537434</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013907039.1">
        <property type="nucleotide sequence ID" value="XM_014051564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WVU4"/>
    <dbReference type="GeneID" id="106537434"/>
    <dbReference type="KEGG" id="tsr:106537434"/>
    <dbReference type="OrthoDB" id="8195456at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090162">
        <property type="term" value="P:establishment of epithelial cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033561">
        <property type="entry name" value="FBF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049390">
        <property type="entry name" value="FBF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33689">
        <property type="entry name" value="FAS-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33689:SF1">
        <property type="entry name" value="FAS-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21007">
        <property type="entry name" value="FBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fas-binding factor 1 C-terminal" evidence="3">
        <location>
            <begin position="354"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="337"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013907039.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="55672" checksum="4C2F05FB1C48C2FD" modified="2020-10-07" version="1">RKESHPARARQDELLGLGTAKKLLERPSTGEPKEFKLDKKYQKQTDKEDALGDDFTFGAYQPTIASTPEGRKSRQQSVRFSSENLSDLKTEQRSKPSPSASNSPVRRTGADWLGLKDEDSDLLPSSPLKEPSKSSARGTTLDMPPFPSEAKHPPPPTTNQGPLTTKQVTEEVIQKKEPAEDDGDSWLNNVLSQKISQTREKIRKRSGSLDVQTDGDHPTSQQVISTQEQHRSAATSDKAARLEQLESFIPWERSQEQSAPTFPSDSRKGTSFGDTTSRASATFWQGHQEIAKVPVNSQALISEPHLLSSSNDADYEKRMAGVQVQFRETLGTYQAEILSAQTRLTELEVQVRRLELEKNQQKLLLESLQRRHQEDLELIENAHRNRVKVIEDSYRQREDRLRQENQELVGQYLSRCQSAEQAKSEILAQHQRRLTELEQEKAKEIDRLQELQRMSILEMRKDHEEQLQRLKQLKSQEIDAVTSATSYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZ81</accession>
    <name>A0A6I9WZ81_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Leucine-rich repeat-containing protein 52</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LRRC52</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907436.1">
        <property type="nucleotide sequence ID" value="XM_014051961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZ81"/>
    <dbReference type="GeneID" id="106537741"/>
    <dbReference type="KEGG" id="tsr:106537741"/>
    <dbReference type="CTD" id="440699"/>
    <dbReference type="OrthoDB" id="4691307at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099104">
        <property type="term" value="F:potassium channel activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000015">
        <property type="entry name" value="Leucine rich repeat containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051432">
        <property type="entry name" value="KCNMA1_auxiliary"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473">
        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473:SF6">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="270"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="14">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013907436.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35660" checksum="30EA1B7D07552ADB" modified="2020-10-07" version="1">MAPGPDLHGNYQDPKKEVQCHIMPLSLSSKNVWMVVLLSRISWTVGSISCPKECTCQYLTVNCTGKLLEKFPTTIPLDTRQLVLAQNKLSSLPSLELHVLSDLVYLDCSSNTLGKDLDFTFVSMIKLIYLDLSFNNLTQVTFGTFSQLSSLMVLKLSDNPSLVEIEKDSFANNTWLRHLDVSRCSLMFIDTSTIRDLPNLRSLGLGENPWFCNCSFMELCSWLKESGITILDPANVTCYSPVFMHGLKMLEEVQEELYYKCYVHFSNQDYLFFGLVGFGIFSAGTVLAWLMGVCAVIYEYLTASGEDDKQEEETEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZS5</accession>
    <name>A0A6I9WZS5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitogen-activated protein kinase kinase kinase 15-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106537875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907626.1">
        <property type="nucleotide sequence ID" value="XM_014052151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZS5"/>
    <dbReference type="GeneID" id="106537875"/>
    <dbReference type="KEGG" id="tsr:106537875"/>
    <dbReference type="OrthoDB" id="275301at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF363">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013907626.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16148" checksum="0D3BB55443823310" modified="2020-10-07" version="1">MEQVPGGSLSALLRSKWGPMKEPTIKFYAKQILEGLKYLHENQIVHRDIKGDNVLINTYSGVVKISDFGTSKRLAGVNPCTETFTEMYNGRIFQFLKNGSLIPEKLEQLSEFVNKHCTAIFIDLHWDCIRSWGLATGQLTDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X014</accession>
    <name>A0A6I9X014_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homeobox protein CDX-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106538580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Caudal homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908574.1">
        <property type="nucleotide sequence ID" value="XM_014053099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X014"/>
    <dbReference type="GeneID" id="106538580"/>
    <dbReference type="KEGG" id="tsr:106538580"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000089">
        <property type="entry name" value="Caudal type homeobox 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006820">
        <property type="entry name" value="Caudal_activation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047152">
        <property type="entry name" value="Caudal_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24332">
        <property type="entry name" value="HOMEOBOX PROTEIN CDX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24332:SF15">
        <property type="entry name" value="HOMEOBOX PROTEIN CDX-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04731">
        <property type="entry name" value="Caudal_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="153"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="155"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013908574.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="31708" checksum="465C846787997584" modified="2020-10-07" version="1">MNFIYVSYILEKEARMYPGSVRCNGSSNLPAQNFPSATPYVDYIGYHHPQSMENRGQPSGTWGTPYGLPREDWGAYGPGTSNTLTSTQINRSSPGQVSFSSADYSSLNSAGSVTLPSLDTMNPEANSPPLSQRHSSYEWMRKTVQSTSTGKTRTREKYRVVYTDHQRLELEKEFHYNRYITIRRKSELAANLRLSERQVKIWFQNRRAKERKLMKKKMTSFDGGSLGSIQSDSGSISPIPVHDPQTQSEMAGTLFPPPPPPPPPLSMNGLQHNGTITRVVVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0L8</accession>
    <name>A0A6I9X0L8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106537661</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907327.1">
        <property type="nucleotide sequence ID" value="XM_014051852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0L8"/>
    <dbReference type="GeneID" id="106537661"/>
    <dbReference type="KEGG" id="tsr:106537661"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000024">
        <property type="entry name" value="Vomeronasal 2, receptor 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="569"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="639"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="684"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="728"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="763"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="789"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="569"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013907327.1"/>
        </source>
    </evidence>
    <sequence length="834" mass="94575" checksum="69AB54DCB8FC7872" modified="2020-10-07" version="1">MGDYIFGGIFSLHSAFYGQGTFEKPPLFFNLITKRIHPKNYQHVLAAVFAVHEINKDPMLLSNITLGFRIVDNYYMGIMTYLYTLQLLTTQERGIYNYICDAQLLAIVGGLDSENSIQISNIVGVYKIPQISYGSFDAVLSDKIRFPFFYRMIPNESLQFKGIIQLLLHFSWKWIGIMAPDNNGGEKFLRFLTIMLSKHDICVSFTQTLATKTASETEIKTEFDKIETIYSQTSANVVVASGDSRDLLMFTIWMDCNAYYKEIPFVTKVLIMTAQWDFTAVNSKGLWFLKPFHGALSFTIHTNNVPGFQDFLDTLDCLYPKGDIFIQEFCLSAFKQGYYNDDMNSVKYEINGRGKGKMKSLPGAVFERVLSGQSYSTYNSVHILAQAFNAIYSYGSKYRHLKKWGESSFLNMQQWQLHRVLKAIGFNNSAGDKIYFNEDQESTDGYDIVNCIIFPNESFSQVKVGRVEQQSLKNLVFTFKDNAVVWNNWFNQTTPHSFCTDNCQTGYYRQLRQGKPVCCYDCIPCPEDMISNTTDAQYCSQCPDDEHSSQGQDECIPKSIDFLSYTETLGILLASSSIILSFITVLILIIFIKLRKTPLVKANNRDITYALLFLLLLCFLSSLLFIGKPRRVTCLLQQIMFGLIFTAAVSSVLAKTITVILVFKAIQPGSKMRTWLKKSVANSIMLLCFLGQFVICVAWLSISPPFPDLDLTSQRTKVIVKCNEGSVAMFYCVLSYMGFLAFISFIVASFARNLPDSFNEAKFITFSLLVFCSVWVSFVPTYLSTKGKYMVAVEIFCILASGAGLLGCIFFPKCYIIIVKPGLNCKDHLIRKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X133</accession>
    <name>A0A6I9X133_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Urocanate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Imidazolonepropionate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">UROC1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-imidazolone-5-propanoate = trans-urocanate + H2O</text>
            <dbReference type="Rhea" id="RHEA:13101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="4.2.1.49"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the urocanase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.49" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013907517.1">
        <property type="nucleotide sequence ID" value="XM_014052042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X133"/>
    <dbReference type="GeneID" id="106537795"/>
    <dbReference type="KEGG" id="tsr:106537795"/>
    <dbReference type="CTD" id="131669"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016153">
        <property type="term" value="F:urocanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1770.10:FF:000002">
        <property type="entry name" value="Urocanate hydratase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1770.10:FF:000003">
        <property type="entry name" value="Urocanate hydratase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10730:FF:000002">
        <property type="entry name" value="Urocanate hydratase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10730">
        <property type="entry name" value="Urocanase like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1770.10">
        <property type="entry name" value="Urocanase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00577">
        <property type="entry name" value="HutU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055351">
        <property type="entry name" value="Urocanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023637">
        <property type="entry name" value="Urocanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035401">
        <property type="entry name" value="Urocanase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038364">
        <property type="entry name" value="Urocanase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023636">
        <property type="entry name" value="Urocanase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035400">
        <property type="entry name" value="Urocanase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035085">
        <property type="entry name" value="Urocanase_Rossmann-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036190">
        <property type="entry name" value="Urocanase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01228">
        <property type="entry name" value="hutU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003820">
        <property type="entry name" value="PRK05414.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12216">
        <property type="entry name" value="UROCANATE HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12216:SF3">
        <property type="entry name" value="UROCANATE HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01175">
        <property type="entry name" value="Urocanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17392">
        <property type="entry name" value="Urocanase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17391">
        <property type="entry name" value="Urocanase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001423">
        <property type="entry name" value="Urocanate_hydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111326">
        <property type="entry name" value="Urocanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01233">
        <property type="entry name" value="UROCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Urocanase N-terminal" evidence="12">
        <location>
            <begin position="85"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Urocanase Rossmann-like" evidence="11">
        <location>
            <begin position="214"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Urocanase C-terminal" evidence="13">
        <location>
            <begin position="440"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013907517.1"/>
        </source>
    </evidence>
    <sequence length="676" mass="74794" checksum="D5690AEE5E31DB2F" modified="2020-10-07" version="1">MSTLKAICAGLPLHPLPENRGRKSGIPHAPVRSPNLTESEEKLALKNSLRYFPPNLHGILAPEFAKELRRYGHIYMYRFCPQIEMRSYPIQEYPCKTKQAAAIMHMIMNNLDPAVAQFPQELVTYGGNGQVFSNWAQFWLVMHYLSEMTEEQTLVMYSGHPLGVFPSHCNAPRLVITNGMVIPNYSSREEYESMFAKGVTMYGQMTAGSYCYIGPQGIVHGTVLTVLNAGRRYLGVENLSGKVFVTSGLGGMSGAQAKAAVIAGCVGVIAEVDEAALLKRHHQGWLMKITRSLDDCIAQLREAKKNKVTLSLGYHGNVVDLWERLVDEFNTTGELLVDLGSDQTSCHNPFNGGYYPVQLSFRDANQLMSNDPIKFQALVKESLRRQIAAINKLSDQGMFFWDYGNAFLLEAQRAGADVAKRGGSKGEFRYPSYVQHIMGDIFSMGFGPFRWVCTSGDPEDLATTDFISTEVLEELIHQGANPSVKQQYYDNIRWIQEARRHNLVVGSQARILYSDQKGRVAIAVAINKGIAEGRIKAPVVLSRDHHDVSGADSPYRETSNIYDGSAFCADMAVQNFVGDAFRGATWVALHNGGGVGWGEVINGGFGLVLDGSTGAEERAKMMLNWDVSNGVARRCWSGNNNAYETICQAMDKEKNLKVTLPHKVLDDSILEQALSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X174</accession>
    <name>A0A6I9X174_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger matrin-type protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZMAT4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907567.1">
        <property type="nucleotide sequence ID" value="XM_014052092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X174"/>
    <dbReference type="GeneID" id="106537830"/>
    <dbReference type="KEGG" id="tsr:106537830"/>
    <dbReference type="CTD" id="79698"/>
    <dbReference type="OrthoDB" id="1925236at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051868">
        <property type="entry name" value="ZN346_ZMAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144:SF3">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144">
        <property type="entry name" value="ZINC FINGER PROTEIN 385B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="8">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013907567.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="22159" checksum="6CD6CF73B1818B71" modified="2020-10-07" version="1">MKSSEIDQELFTESYCRVCSAQLISESQRVAHYESRKHASKVRLYYMLHPIDGGCPAKKLRSENGNDEESVDKNKCCTLCNMSFTSAVVAESHYQGKIHAKRLKLLLGVQPTLKATETNLSSLKEPQVDSSPAVSPPHQRRDSDRYCQLCAAWFNNPMMAQQHYDGKKHKKNAARADLLEQLGKTLDLGEVRGKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2R6</accession>
    <name>A0A6I9X2R6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Protein N-terminal glutamine amidohydrolase</fullName>
            <ecNumber evidence="4 8">3.5.1.122</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein NH2-terminal glutamine deamidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">WDYHV1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>N-terminal L-glutaminyl-[protein] + H2O = N-terminal L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:50680"/>
            <dbReference type="Rhea" id="RHEA-COMP:12668"/>
            <dbReference type="Rhea" id="RHEA-COMP:12777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64721"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="EC" id="3.5.1.122"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NTAQ1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.122" evidence="4 8"/>
    <dbReference type="RefSeq" id="XP_013909569.1">
        <property type="nucleotide sequence ID" value="XM_014054094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2R6"/>
    <dbReference type="GeneID" id="106539320"/>
    <dbReference type="KEGG" id="tsr:106539320"/>
    <dbReference type="CTD" id="55093"/>
    <dbReference type="OrthoDB" id="191192at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.620.10:FF:000001">
        <property type="entry name" value="Blast:Protein N-terminal glutamine amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.10">
        <property type="entry name" value="Protein N-terminal glutamine amidohydrolase, alpha beta roll"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037132">
        <property type="entry name" value="N_Gln_amidohydro_ab_roll_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039733">
        <property type="entry name" value="NTAQ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023128">
        <property type="entry name" value="Prot_N_Gln_amidohydro_ab_roll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13035">
        <property type="entry name" value="PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13035:SF0">
        <property type="entry name" value="PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09764">
        <property type="entry name" value="Nt_Gln_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein N-terminal glutamine amidohydrolase alpha beta roll" evidence="9">
        <location>
            <begin position="21"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013909569.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="24116" checksum="31DF564932BB4038" modified="2020-10-07" version="1">MAEAVMARYDPVVSSRSVCVYTSCYCEENIWKLCEHIRSKNQYPLEEFYAVFISNDRRMVPLWKQQAGREDQPVIWDYHVILLHVSKGDQNYIYDLDTVLPFPCPFGTYIEEAFKSDSIIDAEFRRKVRLVRADTYLKNFASDRSHMKDASGNWRKSPPLYPCIETADYKMNLDDFISMNPKAGWGSVLKLPDFVQKFGSQTYEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7E8</accession>
    <name>A0A6I9X7E8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proenkephalin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-neoendorphin-dynorphin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Preprodynorphin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PDYN</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opioid activity, it is 700 times more potent than Leu-enkephalin.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Leumorphin has a typical opioid activity and may have anti-apoptotic effect.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the opioid neuropeptide precursor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909762.1">
        <property type="nucleotide sequence ID" value="XM_014054287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7E8"/>
    <dbReference type="GeneID" id="106539487"/>
    <dbReference type="KEGG" id="tsr:106539487"/>
    <dbReference type="CTD" id="5173"/>
    <dbReference type="OrthoDB" id="8912385at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001515">
        <property type="term" value="F:opioid peptide activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031628">
        <property type="term" value="F:opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007600">
        <property type="term" value="P:sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006024">
        <property type="entry name" value="Opioid_neupept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000750">
        <property type="entry name" value="Proenkphlin_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438">
        <property type="entry name" value="PROENKEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438:SF4">
        <property type="entry name" value="PROENKEPHALIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01160">
        <property type="entry name" value="Opiods_neuropep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01028">
        <property type="entry name" value="OPIOIDPRCRSR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01030">
        <property type="entry name" value="PENKBPRCRSR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0257">Endorphin</keyword>
    <keyword evidence="7" id="KW-0529">Neurotransmitter</keyword>
    <keyword evidence="8" id="KW-0555">Opioid peptide</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026723550" description="Proenkephalin-B" evidence="17">
        <location>
            <begin position="18"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013909762.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="27038" checksum="A1F110EDCCE78F87" modified="2020-10-07" version="1">MDWQLWGLVFYLHVAFSASWECVSHCSLCILHNQDGEKPINPLGCALGCQHPSVTGVEWQRCERILSLLTSFPMEEEEEEEEEEEGEAGRSQSVLTKGDASRSFGRFFPKMVERARPEAGGDNPEMLEIRGLVSHREEEEEEEPDPASPKAERKRYGGFLRKFPKRGSWVAGTEEEEDGGDQEDLHKRYGGFMRRIRPKLKWEKQKRYGGFLRRQFKVTTRSEEGPKGASEEAADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9D8</accession>
    <name>A0A6I9X9D8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Luc7-like protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LUC7L3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912214.1">
        <property type="nucleotide sequence ID" value="XM_014056739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9D8"/>
    <dbReference type="GeneID" id="106541337"/>
    <dbReference type="KEGG" id="tsr:106541337"/>
    <dbReference type="CTD" id="51747"/>
    <dbReference type="OrthoDB" id="10266921at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="137"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="318"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="374"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912214.1"/>
        </source>
    </evidence>
    <sequence length="492" mass="58555" checksum="2CE977B30EA3A395" modified="2020-10-07" version="1">MISAAQLLDELMGRDRNLAPDEKRSNVRWDHESVCKYYLCGFCPAELFTNTRSDLGPCEKIHDENLRKQYILDIDRYEKSSRFMKVGYEREFLRYLQSLLAEVERRIRRGHARLALSQTQQSSGQAAGPTGKNEEKIQVLTDKIDVLLQQIEELGSEGKVEEAQGMMKLVEQLKEERELLRSTTSTIESFAAQEKQMEVCEVCGAFLIVGDAQSRVDDHLMGKQHMGYAKIKATVEELKEKLRKRTEEPERDDRLKKEKLEREEREREREREEKERKRRREEEEKEKERARDRERRKRSRSRSRHSSRTSDRRGSRSRDHKRSRSKERRRSRSRDRRRSRSHDRSDRKHRSRSRDRRRSKSRDRKSYKHRSKSREREQERKSKEKEKRGSDDKKSSVKSSSREKQSEDTSMDSKEGDAKNEVNGTSEDIKSEVQRKYVQMKMDLRQVRRDTKATSEGKHSVVLQNLLRYIVLYRLFCSRFISPLACLLGTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBB2</accession>
    <name>A0A6I9XBB2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106540842</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013911581.1">
        <property type="nucleotide sequence ID" value="XM_014056106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBB2"/>
    <dbReference type="GeneID" id="106540842"/>
    <dbReference type="KEGG" id="tsr:106540842"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF93">
        <property type="entry name" value="NUAK FAMILY SNF1-LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="45"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013911581.1"/>
        </source>
    </evidence>
    <sequence length="277" mass="31930" checksum="33627D3C88C520AA" modified="2020-10-07" version="1">MERVPPCEAPAVSPFREGLFHFPKAPVKKQAVKRHYHKHNLRHRYEFLETLGKGTYGKVKKARERSGKLVAIKSIRKDKIKDEQDLLHIRREIEIMSSLNHPHIIAVHEVFENSSKIVIVMEYASKGDLYDYISERQRLTEQEARHFFRQVVSAIYYCHKNGIVHRDLKLENILLDANGNIKIADFGLSNVFQQDRLLQTFCGSPLYASPEIVNGRPYKGPEVDSWSLGVLLYILIHGTMPFDSHDYKTLVKQITRGDYKEPTKLSGKLKSDKGFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBL5</accession>
    <name>A0A6I9XBL5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proline-rich protein 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PRR18</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911232.1">
        <property type="nucleotide sequence ID" value="XM_014055757.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540582"/>
    <dbReference type="KEGG" id="tsr:106540582"/>
    <dbReference type="CTD" id="285800"/>
    <dbReference type="OrthoDB" id="8963734at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031369">
        <property type="entry name" value="PRR18"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15671">
        <property type="entry name" value="PRR18"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911232.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="31897" checksum="660E62E17F2AD140" modified="2020-10-07" version="1">MQKTQPGAARSFQPVAANSNGGKSPGEARTPRRLKATVARRVPSGCPEDGGAMPEAARSLLALLALTVACXSLEGDGGSEAAARPRGRCFSRLRRCKTRIASGGRLSETRSEASPRPPGAAQRLSRGRSGPPAAGTRSYESLCAAPGGRGEASLRFSLSLPPEAIRVLQRRSLEKQRPRGRVARFASPDASRCPLAGTSSPEGDLRALLQVSLLNERHRYDDVEYEEDEAAGDPGAFAADEGLLIPARERKKAPHFLLMGRTVEHGSGFRPLVFPGEVGVVFFFVPFLRKHPGGKPCPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCP6</accession>
    <name>A0A6I9XCP6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NAD(P)H dehydrogenase [quinone] 1</fullName>
            <ecNumber evidence="4">1.6.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Azoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">DT-diaphorase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Menadione reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">NAD(P)H:quinone oxidoreductase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Phylloquinone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Quinone reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">NQO1</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:46161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a quinone + NADPH + H(+) = a quinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:46165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>menadione + NADH + H(+) = menadiol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:69695"/>
            <dbReference type="ChEBI" id="CHEBI:6746"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28869"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:69696"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>ubiquinone-10 + NADH + H(+) = ubiquinol-10 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:61984"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46245"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:61985"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer. Interacts with PDLIM4 isoform 2; this interaction stabilizes PDLIM4 isoform 2 in response to oxidative stress and protects it from ubiquitin-independent degradation by the core 20S proteasome. Interacts with TP73 (via SAM domain); this interaction is NADH-dependent, stabilizes TP73 in response to oxidative stress and protects it from ubiquitin-independent degradation by the 20S proteasome. Interacts with TP53; this interaction is NADH-dependent, stabilizes TP53 in response to oxidative stress and protects it from ubiquitin-independent degradation by the 20S proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)H dehydrogenase (quinone) family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013913364.1">
        <property type="nucleotide sequence ID" value="XM_014057889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCP6"/>
    <dbReference type="GeneID" id="106542222"/>
    <dbReference type="KEGG" id="tsr:106542222"/>
    <dbReference type="CTD" id="1728"/>
    <dbReference type="OrthoDB" id="26889at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042373">
        <property type="term" value="P:vitamin K metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000029">
        <property type="entry name" value="NAD(P)H dehydrogenase [quinone] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003680">
        <property type="entry name" value="Flavodoxin_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051545">
        <property type="entry name" value="NAD(P)H_dehydrogenase_qn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10204">
        <property type="entry name" value="NAD P H OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10204:SF1">
        <property type="entry name" value="NAD(P)H DEHYDROGENASE [QUINONE] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02525">
        <property type="entry name" value="Flavodoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Flavodoxin-like fold" evidence="27">
        <location>
            <begin position="5"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="251"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00046551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF02525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013913364.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31800" checksum="269280FB1E79364C" modified="2020-10-07" version="1">MAARRALIVLAHQEPGSFNHALQRAAVEALESGGWSVAVSDLYAQHFQPVLSRADFAGDPDDPRNLNYAKEAGLAWEEGRLSSDILGEVEKLEAADLVIFQFPLHWLGLPAILKGWFDRILIQGFAYSYGAMYDQGPFQKKKAVLSFTTAGMGSMYTPAGINGDINIMLWPIQSGTLHFCGFQVLAPQIAFSIGQRTPEDVRSQILEDWKKRLATIWEEEPLRFAPNCLFELNPAQGFVLKEDVREEQEREPYGLTVGQHLGKPFPPDSQVKAQEKRLSRWEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDP7</accession>
    <name>A0A6I9XDP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein aurora borealis</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BORA</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BORA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909263.1">
        <property type="nucleotide sequence ID" value="XM_014053788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDP7"/>
    <dbReference type="GeneID" id="106539103"/>
    <dbReference type="KEGG" id="tsr:106539103"/>
    <dbReference type="CTD" id="79866"/>
    <dbReference type="OrthoDB" id="10020858at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023252">
        <property type="entry name" value="Aurora_borealis_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14728">
        <property type="entry name" value="PROTEIN AURORA BOREALIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14728:SF2">
        <property type="entry name" value="PROTEIN AURORA BOREALIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15280">
        <property type="entry name" value="BORA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02038">
        <property type="entry name" value="AURORABORA"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909263.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="52814" checksum="F6379AD80EF0BAE6" modified="2020-10-07" version="1">MWSIDELALIDPVEINPEDIHRQAFYLSQTRIDREVEERRQRAINEFFTKSTIVPSPWTDPIGKQSSQLNSTRCVELNDASPIGREIIVQPGKNNAACQTVLSLPVDFDLEKILGDYFKADESADQSQENLSTSSLRRKLFLDENGLSSESPSPSLGLRNGPASLGVLCSIDLSPVRCRSPLSSDSSGHLSSSPIRGNARTYSLGSLPSPSLARRSPANLASPTFSPVGIQLRKTPMAGQRQFTFRSPDLPSVSNACRQAPPTSSRSPHMECSPLRSHSPMRLLSCWGTALYQSSFLRLPCTPENFQEELAEAAEATWAGSPRAGLTPINEDIPLAGSPSEMAAGALSPETPEGLALRPCLDSSGEKRTVEMADPEMLDENSVKGSSECDGGAMTGLSMERSCISMDPLAESSASPCESSSLQMDSGYNTSSLMDTAGGELSCREHSAFEAQRKTHVFRMKQGKSKTGHWAENACQPSSLFLEVPLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEF7</accession>
    <name>A0A6I9XEF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106542738</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914029.1">
        <property type="nucleotide sequence ID" value="XM_014058554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEF7"/>
    <dbReference type="GeneID" id="106542738"/>
    <dbReference type="KEGG" id="tsr:106542738"/>
    <dbReference type="OrthoDB" id="432835at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03165">
        <property type="entry name" value="NET-5_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000006">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF40">
        <property type="entry name" value="TETRASPANIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914029.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="36747" checksum="D7FD465A2B9B1F2F" modified="2020-10-07" version="1">MRRSSTDESTYHRSPSVDSKDYSFPNGAFRRYSSMYGGQFGAASNSFFGFHTNPGPKATKAKYTSPKGNKYVVFYLDLSFIFLLELKRCSMAHNCLQCIKYLMFVFNLLFWLGGCGILGVGIWLAVTQGNFATLSSSFPSLSAANLLIITGTFVMIIGFVGCIGAIKENKCLLLSFFIMLLIIFVLELTVVILFFVYTDKIDKYAQRDLKRGLHLYGTDGNIGLTNAWSIIQTDFRCCGVSNYTDWFEVYNTTRVPDSCCLEFSENCGLHSPGTWWKAPCYETVKLWLQENLLAVGIFGLCTALVQILGLTFAMTMYCQVVKADTYCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XF28</accession>
    <name>A0A6I9XF28_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA mismatch repair protein Msh6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106541525</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912482.1">
        <property type="nucleotide sequence ID" value="XM_014057007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XF28"/>
    <dbReference type="GeneID" id="106541525"/>
    <dbReference type="KEGG" id="tsr:106541525"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013912482.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16453" checksum="A0058DEAE0BED592" modified="2020-10-07" version="1">MDELGRGTATFDGTAIASAVVKELSENIKCRTMFSTHYHSLVEDYSHSLSVRLGHMACMVENECEDPSQETITFLYKFVKGACPKSYGFNAARLADIPEEVIQKGHKKAKEFEKSVLSMKVFRNLCWIAEGALAAKDYLDKLTLLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFQ7</accession>
    <name>A0A6I9XFQ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glutamate receptor-interacting protein 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106541716</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912707.1">
        <property type="nucleotide sequence ID" value="XM_014057232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFQ7"/>
    <dbReference type="GeneID" id="106541716"/>
    <dbReference type="KEGG" id="tsr:106541716"/>
    <dbReference type="OrthoDB" id="75502at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098887">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06687">
        <property type="entry name" value="PDZ1_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06681">
        <property type="entry name" value="PDZ2_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06684">
        <property type="entry name" value="PDZ3_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06686">
        <property type="entry name" value="PDZ4_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06682">
        <property type="entry name" value="PDZ5_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06683">
        <property type="entry name" value="PDZ6_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000021">
        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000022">
        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000023">
        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000025">
        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000031">
        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000034">
        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043545">
        <property type="entry name" value="GRIP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46227:SF4">
        <property type="entry name" value="GLUTAMATE RECEPTOR-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46227">
        <property type="entry name" value="GLUTAMATE RECEPTOR-INTERACTING PROTEIN GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="87"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="187"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="287"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="496"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="597"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="695"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="443"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="779"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="853"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="443"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="779"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013912707.1"/>
        </source>
    </evidence>
    <sequence length="917" mass="100340" checksum="2BCADA2B67C629C5" modified="2020-10-07" version="1">MKKIFKVFQVVQKSSRRKRNRNKAEYGVQEELVTQEYCDRQIVLCSLHLCVTDDGPYSKGSKDSGGLDTALVSRRQSIPEEFRGVTIVELIKKEGSTLGLTIAGGTDKDGKPRVSNLRPGGLAARSDQLNVGDYIKSVNGINLTKLRHDEIISLLKNVGERVILEVEYELPLAAPDNSAGIIPKTIEVTLYKEGNSFGFVLRGGAHEDWHKSRPLLLTYVRPGGPADREGSLKIGDRLLSVDGIPLHGVNHADALNILRQCNQEALFQIEYDVSIMDMVANASGPLLVEIAKAPGSALGITLSMNTHRNKQVIIIEKIKPASVGDRCGALHVGDHILSIDGTSTEHCSLLEATQLLAATSETVKLEILPAHYSRLPLKPLDTVKVQKSEHHHCWDPCVSYCHTHHPGHCKTPTWNQTISQDYCKSLVASNFASPTMNTSGLAYQNSNTLPRSNHPMSPRATLMRRRQRKKEHKSSLSLASSTVGPGGQIVHTETTEVILRGDPLNGFGLQLQGGIFATETLSSPPLIRFIEPDSPAERCGLLQVGDRVLSINSIATEDGTMEEANQLLRDAALTNKVVLEIEFDVAESVIPSSGTFHVKLPKKRGVELGITISSSRKSGEPLIISDIKKGSVAHRTGTLEPGDKLLAIDNIRLDNCSMEDAVQILKQCEELVKLKIRKDEDNSDEQETTGAIIYTVELKRYGGPLGITISGTEEPFDPIVISGLTKRGLAERTGAIHVGDRILAINNVSLKGKPLSEAIHLLQMAGETVTLKIKKQTERTFPQKSGSMTEVSDLEDDLTDSQKTSKLSEVYSTTIPSVDSAMESWDGSGIDTGYGSQGTYLPQAVSIALHPHEWRSSRQKSSTPPGDARKSYPFAHGGFNEEDWEKPSRYVLLGKDRGEMNAYCYLLQESNIQACFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGG2</accession>
    <name>A0A6I9XGG2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 73-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106539768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910128.1">
        <property type="nucleotide sequence ID" value="XM_014054653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGG2"/>
    <dbReference type="GeneID" id="106539768"/>
    <dbReference type="KEGG" id="tsr:106539768"/>
    <dbReference type="OrthoDB" id="6145717at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031650">
        <property type="entry name" value="CCDC73"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28660">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28660:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15818">
        <property type="entry name" value="CCDC73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910128.1"/>
        </source>
    </evidence>
    <sequence length="109" mass="12881" checksum="35C651445DD4B841" modified="2020-10-07" version="1">MDEDLDKEIAAYSLQNSSEALLSIQLLDFKTTFLEAVEELRMRRDSENLYEKQISRHVAEKQDLVWQKEAVQHEKEALKKQHAEAITTLKKQINYSNLQYYYSVPTNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGP6</accession>
    <name>A0A6I9XGP6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-acylglycerol-3-phosphate O-acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106539831</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013910208.1">
        <property type="nucleotide sequence ID" value="XM_014054733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGP6"/>
    <dbReference type="GeneID" id="106539831"/>
    <dbReference type="KEGG" id="tsr:106539831"/>
    <dbReference type="OrthoDB" id="202234at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF65">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="6">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013910208.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16452" checksum="63A98DD5191558AB" modified="2020-10-07" version="1">MEISPGQWLLILFLVAIPLLYEWSATFKYFCKMAFYNSYILFLAIIAIPICAVRGRDVENMRVLRFMLLHIKYLYGIKIEVRGTENFNIKEPYVVVSNHQSSLDLLGRTGGRCGGWEGAKNLIWALLVHLFLLVGFFLIDVAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGR6</accession>
    <name>A0A6I9XGR6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Transcription intermediary factor 1-beta isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TRIM28</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913311.1">
        <property type="nucleotide sequence ID" value="XM_014057836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGR6"/>
    <dbReference type="GeneID" id="106542182"/>
    <dbReference type="KEGG" id="tsr:106542182"/>
    <dbReference type="CTD" id="10155"/>
    <dbReference type="OrthoDB" id="1870062at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19846">
        <property type="entry name" value="Bbox1_TIF1b_C-VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19829">
        <property type="entry name" value="Bbox2_TIF1b_C-VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15623">
        <property type="entry name" value="PHD_TIF1beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16765">
        <property type="entry name" value="RING-HC_TIF1beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000074">
        <property type="entry name" value="Tripartite motif containing 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037373">
        <property type="entry name" value="KAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047059">
        <property type="entry name" value="TIF1b_Bbox1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047058">
        <property type="entry name" value="TIF1b_Bbox2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042713">
        <property type="entry name" value="TIF1beta_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF8">
        <property type="entry name" value="TRIPARTITE MOTIF CONTAINING 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="44"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="13">
        <location>
            <begin position="120"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="13">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="623"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="803"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013913311.1"/>
        </source>
    </evidence>
    <sequence length="843" mass="92534" checksum="0102671C0729247B" modified="2020-10-07" version="1">MSASGTATVSAPAEAGGGGVAGDKQRSSSSAPLNVEGIDLLEKCGVCRERLRSERDPRLLPCLHTVCKECIKVEAVSGGNNKDGQVVDCPVCKHQCYLKDIVENYFLRDGDTEALGDARDANQCCTSCEDNAPATSFCVECQEPLCETCVEAHQRVKYTKDHTVRSTGSSKSQAREKTIYCSVHKLEPLVLFCDTCDTLTCRDCQLNAHKDHQYQFLDDAVKNQRKMLASLVKRLGDKNANLQKSTKEVRTSIRQVADVQKRVQVDVKMSILQIMKELNKRAKVLVSEAQKVTEGQQEKLERQHWAMTKLQRHQEHILRFANWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGDMKFQWDLNAWIKSAETFGQIVSERSGLPVTANAQTPQQRTNTASAASGVAVRQGSVPPSQGVQQMSLQTSIMNKGALLQPVLPSNSQRQAIPDCYINDASASSAGQPMDIGDGFGVEGSYSGEVSGMKRTRVQDGETGLVRKVPRVSLERLDVDLTSDNQPPVFKVFPGNSREDYNLIVIEQGGQGVAGLPNAVKEEQMEAAIEHPTSAVDTKDTKPVILPQDALLPEGSAAPRLISPTGSTSSAMEMQSVHSHDGTANGEENACCRICLKTGAVVMCDSCRKCYHLDCHLPALQEIPGQDWKCLLCTDPVIAIGEDGTELAAVFKDGEAKMLSSVDQKRCERVLLELLCSEPCRPLHRLSNAPEGQNTIDLTLIRAKLQRKLSPPYASPDEFADDVWKMFRQFNKLTEDKEDVQSIIVLQRFFEAKLSAAFGDRKFSALREPINIDEPQKENTLASRVSPVTDPGEAMSSKADSSGDEGMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHH1</accession>
    <name>A0A6I9XHH1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Charged multivesicular body protein 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7 8 9" type="primary">CHMP2A</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913315.1">
        <property type="nucleotide sequence ID" value="XM_014057840.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913316.1">
        <property type="nucleotide sequence ID" value="XM_014057841.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913317.1">
        <property type="nucleotide sequence ID" value="XM_014057842.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913318.1">
        <property type="nucleotide sequence ID" value="XM_014057843.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913319.1">
        <property type="nucleotide sequence ID" value="XM_014057844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHH1"/>
    <dbReference type="GeneID" id="106542184"/>
    <dbReference type="KEGG" id="tsr:106542184"/>
    <dbReference type="CTD" id="27243"/>
    <dbReference type="OrthoDB" id="10252926at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="12"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013913317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013913319.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24968" checksum="F32BAA252AEE99BA" modified="2020-10-07" version="1">MDLLFGRRKTPEEMLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVKIMAKDLVRTRRYVKKFILMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMATMNRQLKLPQIQKIMMEFEKQSEIMEMKEEMMNDAIDDAMGDEEDEEESDAVVSQVLDELGLTLTDELSNLPSTGGSLSVAGGKKAEASAALADADADLEERLKNLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHJ2</accession>
    <name>A0A6I9XHJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Beta-2-syntrophin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">59 kDa dystrophin-associated protein A1 basic component 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Syntrophin-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Syntrophin-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">SNTB2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. May play a role in the regulation of secretory granules via its interaction with PTPRN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the syntrophin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913342.1">
        <property type="nucleotide sequence ID" value="XM_014057867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHJ2"/>
    <dbReference type="GeneID" id="106542206"/>
    <dbReference type="KEGG" id="tsr:106542206"/>
    <dbReference type="CTD" id="6645"/>
    <dbReference type="OrthoDB" id="409749at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06801">
        <property type="entry name" value="PDZ_syntrophin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01258">
        <property type="entry name" value="PHsplit_syntrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000052">
        <property type="entry name" value="Syntrophin beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000251">
        <property type="entry name" value="Syntrophin beta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041428">
        <property type="entry name" value="PHsplit_syntrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015482">
        <property type="entry name" value="Syntrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055108">
        <property type="entry name" value="Syntrophin_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554:SF8">
        <property type="entry name" value="BETA-2-SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554">
        <property type="entry name" value="SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18012">
        <property type="entry name" value="PH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23012">
        <property type="entry name" value="Syntrophin_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="23">
        <location>
            <begin position="91"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="22">
        <location>
            <begin position="303"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="35"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00075427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013913342.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="55726" checksum="EE08063C927BD728" modified="2020-10-07" version="1">MAVWTRARKAGLVEVLLRERWVRAVAELSGEALALSSEPEPRNGLPNGASSAAAAAAASPSASPERGAGAPSPPSPPRGSAPGSPPAGVRRVRVVKAEAGGLGISIKGGRENRMPVLISRIFPGLAAERCGALHLGDAILAVNGLDLRDATHDQAVQALKRAGREVLLEVKYMREATPYIKKPSLVSDLPWDGAFPQSPSLSLSLSEESGSPKHQSPPQDRKVIPLKMCYAARNLSMPDLENRLIELHSPDSRNALILRCKDTATAHSWFTAIHSNIMALLPQVLAELNAMLGASHAPGSSREVKQIAWLAEQARLDGGRQHWRPVLLAVTEKDLLLFDSVPWTRDAWASPCHSYPLVATRLVHSGCRSPCLGSDLSFATRSGSRQGIEMHVFRVETHRDLSTWTRTLVQGCHAATELIKEVSVGCVWNSQDVRLTIHYENGFTVSSEGTGSTGLLLRYPFEKLKMSADDGVRNLYLDFGGPEGELALEMHSCPKPLVFVLHTFLSAKVTRLGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI50</accession>
    <name>A0A6I9XI50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="20">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25 26 27 28 29 30" type="primary">FYN</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25 26 27">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 31">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="20"/>
    <dbReference type="RefSeq" id="XP_013910603.1">
        <property type="nucleotide sequence ID" value="XM_014055128.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910604.1">
        <property type="nucleotide sequence ID" value="XM_014055129.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910605.1">
        <property type="nucleotide sequence ID" value="XM_014055130.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910606.1">
        <property type="nucleotide sequence ID" value="XM_014055131.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910607.1">
        <property type="nucleotide sequence ID" value="XM_014055132.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910608.1">
        <property type="nucleotide sequence ID" value="XM_014055133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI50"/>
    <dbReference type="GeneID" id="106540113"/>
    <dbReference type="KEGG" id="tsr:106540113"/>
    <dbReference type="CTD" id="2534"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05070">
        <property type="entry name" value="PTKc_Fyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10418">
        <property type="entry name" value="SH2_Src_Fyn_isoform_a_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12006">
        <property type="entry name" value="SH3_Fyn_Yrk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000553">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000016">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tyrosine-protein kinase Fyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000120">
        <property type="entry name" value="Tyrosine-protein kinase Fyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047924">
        <property type="entry name" value="Fyn/Yrk_SH2"/>
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        <property type="entry name" value="Fyn/Yrk_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 20 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 17" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 18" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 20" id="KW-0829">Tyrosine-protein kinase</keyword>
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        <location>
            <begin position="82"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="21">
        <location>
            <begin position="149"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="271"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013910603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013910604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013910605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013910606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013910607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013910608.1"/>
        </source>
    </evidence>
    <sequence length="537" mass="60637" checksum="0BA9C2552D8A33D8" modified="2020-10-07" version="1">MGCVQCKDKEATKLTDERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFPTAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNNSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDSGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMGKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJV0</accession>
    <name>A0A6I9XJV0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 41 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">VPS41</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914291.1">
        <property type="nucleotide sequence ID" value="XM_014058816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJV0"/>
    <dbReference type="GeneID" id="106542945"/>
    <dbReference type="KEGG" id="tsr:106542945"/>
    <dbReference type="CTD" id="27072"/>
    <dbReference type="OrthoDB" id="244107at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16690">
        <property type="entry name" value="RING-H2_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000247">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 41 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057779">
        <property type="entry name" value="Znf_RING_Vps41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23555">
        <property type="entry name" value="zf-RING_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="106"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="329"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013914291.1"/>
        </source>
    </evidence>
    <sequence length="392" mass="45948" checksum="33E01A29B9251CE3" modified="2020-10-07" version="1">MVLHEFLESDYQGFATLIKEWPGNLYNNPVIVHAVLDHLKKDPQNRTLLKTLAELYTYDQRYRKALEIYLTLRHKDVFQLIHKHNLFSSIRDKIVLLMEFDSEKAVDMFLDNEDKISIDKVVEELKDRPELQHVYLHKLFKRDHHKGQKYHEKQISLYAEYDRPNLLPFLKNSTHCPLEMALEICQQRLFVEETVYLLSRMGNSRSALKMIMEDLNDVDKAIEFAKEQDDAELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRIKESMKIPNLRDSLVKILQDYNLQILLREGCKKILVADTLSLLKKMHRTQMRGVLVDEENICEICLSPILPTDASKSYNVIVFHCRHMFHKECFPEPNTESSLHFCNICSAKHRGPGSAILEMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLK0</accession>
    <name>A0A6I9XLK0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GNG7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916719.1">
        <property type="nucleotide sequence ID" value="XM_014061244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLK0"/>
    <dbReference type="GeneID" id="106544848"/>
    <dbReference type="KEGG" id="tsr:106544848"/>
    <dbReference type="CTD" id="2788"/>
    <dbReference type="OrthoDB" id="6264244at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013916719.1"/>
        </source>
    </evidence>
    <sequence length="68" mass="7646" checksum="BF52DB1E8CAEA61B" modified="2020-10-07" version="1">MSATNNIAQARKLVEQLRIEAGMERIKVSKASLELMNYCEQQARNDPLLVGLPTSENPFKDKKPCTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM58</accession>
    <name>A0A6I9XM58_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor MafG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">V-maf musculoaponeurotic fibrosarcoma oncogene homolog G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">MAFG</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916963.1">
        <property type="nucleotide sequence ID" value="XM_014061488.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916964.1">
        <property type="nucleotide sequence ID" value="XM_014061489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM58"/>
    <dbReference type="GeneID" id="106545040"/>
    <dbReference type="KEGG" id="tsr:106545040"/>
    <dbReference type="CTD" id="4097"/>
    <dbReference type="OrthoDB" id="5974330at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045604">
        <property type="term" value="P:regulation of epidermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14717">
        <property type="entry name" value="bZIP_Maf_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000011">
        <property type="entry name" value="Transcription factor MafG, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="14">
        <location>
            <begin position="51"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013916963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013916964.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18019" checksum="C7FE1C3F8C512C6C" modified="2020-10-07" version="1">MTTPNKGNKALKVKREPGENGTSLTDEELVTMSVRELNQHLRGLSKEEIIQLKQRRRTLKNRGYAASCRVKRVTQKEELEKQKAELQQEVEKLASENASMKMELDALRSKYEALQNFARTVARSPVTPARGPLASSMGPLVPGKVATTSVITIVKSKTDARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNS7</accession>
    <name>A0A6I9XNS7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit 1C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PPP1R1C</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein phosphatase inhibitor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912393.1">
        <property type="nucleotide sequence ID" value="XM_014056918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNS7"/>
    <dbReference type="GeneID" id="106541455"/>
    <dbReference type="KEGG" id="tsr:106541455"/>
    <dbReference type="CTD" id="151242"/>
    <dbReference type="OrthoDB" id="9940275at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008466">
        <property type="entry name" value="PPP1R1A/B/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15417:SF5">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15417">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR AND DOPAMINE- AND CAMP-REGULATED NEURONAL PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05395">
        <property type="entry name" value="DARPP-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912393.1"/>
        </source>
    </evidence>
    <sequence length="106" mass="12087" checksum="325B6F0D8E96D3ED" modified="2020-10-07" version="1">MEPNSPKKIQFAVPLFQSQIDSEAAEQIRKRRPTPASLVIMNDSSPEIDEKRMNNNSQDESQNVSPKQRKQSVYTAPAMKGIKHLKTQKDLAFPEEDEGVCERDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRB6</accession>
    <name>A0A6I9XRB6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RNA polymerase II elongation factor ELL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ELL2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913223.1">
        <property type="nucleotide sequence ID" value="XM_014057748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRB6"/>
    <dbReference type="GeneID" id="106542099"/>
    <dbReference type="KEGG" id="tsr:106542099"/>
    <dbReference type="CTD" id="22936"/>
    <dbReference type="OrthoDB" id="6284217at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2670:FF:000002">
        <property type="entry name" value="RNA polymerase II elongation factor ELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF8">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="9">
        <location>
            <begin position="525"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="289"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="428"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="336"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="387"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="456"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913223.1"/>
        </source>
    </evidence>
    <sequence length="639" mass="72187" checksum="18B130BC4AA64FE1" modified="2020-10-07" version="1">MASLREGGRYGVSCGRVTQDNITVLHVKLTETAFRALENYQGSKNLIPSRPSIQFQGLQGLVKIPKTDVPNEVHTFDFYLSNVGKDNPQGSFDCVQQTVSSTGSSQLSCLGFIQDKITVCATNDSYQMTRERMTQAEEESRNRSTKVIKPGGPYVGKRVQIRKAPQSIPDAVPERKRSTPINPANTIRKTHTNNSISQRPYKDRIIHLLALKTYKKPELLARLLKDGVSQKDRNSLAAILQQVANLNPKDNSYTLKEYVYKEVQKDWPGYNEIDRQSLELILSRKLHSSQNATSTSHLGSPLRTVKEVASSSPSQKRLLDSDFIDPLMNKKPRISHLTNRVQPTLSSHLPVSGEKSAETSQPPPPPAVAAAPTAPPFVSTCLPVSNPPQTASSNSNSPSTPEGRGTQDLPVDTLSQNGNIYEDQHQKYTSRTPLEIPAPSESLSVCPKSTDEKHSSLYKKSKKKSKKHKEKDQIKDHNTGNEDKKMRNCESHEITKLKNSSQKMSEGVKKTCTATTDPLSTSELRDYFVKYIAIVSYEQRQSYKDDFNAEYDEYRTLHARMESVTRRFMKLDAQRKLLSPGTKEYQVLHEEVLQEYRKIKQSSPNYHEEKYRCEYLHNKLAHIKRLIGEFDQQQAGSWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT35</accession>
    <name>A0A6I9XT35_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myb-like protein X</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106545003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916916.1">
        <property type="nucleotide sequence ID" value="XM_014061441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT35"/>
    <dbReference type="GeneID" id="106545003"/>
    <dbReference type="KEGG" id="tsr:106545003"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916916.1"/>
        </source>
    </evidence>
    <sequence length="551" mass="61301" checksum="79F2710F1D0A82F0" modified="2020-10-07" version="1">MEPEEGRKEDNAGNERLNEESTEGAEPLLLKAAELEGQDGEILGDGELNQGREAVSGEDGNYTEGMAEPEPREVKMGEKSSLRELKSNAESEELGGEGESEEKEHEEIRIVEESRTLEMDEKVDLAKSEDQREKEVHAYTEGKADYPHNLEKAEVFILDQKDSEGGTEKEIVETTEIQKEDNDLSRLGQGGNLEITEEGKDVAADATSVDQKQTEESVELGEEQNKVYIKAEDEEIAGVEQSLIARETETSVETQEKPEQALENLREPGEITGKRDATMADEEATTLHQTKVDKLVEGSLGESGKPVGEQPLTVEAKAEYFPVVVETEEVRIGKVTTEEVVIIPVEDETKDLMETSEEVMGEKDLAAMEAVAVVEVENLVDISETQMGKEDMTLIVESREKTMEELIAEKDVEQEKPKPVTQRNKKPWKPDEEDEEFTANEEEGEDEEETIDAEEKLEGNVDHSKELDDLAKEGELPMEELLQKYAVAYASDFEEEASHTSSEALEPTSSEYEEESEEENSTSHSGIEERGSSGPCKYTAYSCSCISRLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTJ6</accession>
    <name>A0A6I9XTJ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Scinderin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Adseverin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SCIN</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914018.1">
        <property type="nucleotide sequence ID" value="XM_014058543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTJ6"/>
    <dbReference type="GeneID" id="106542729"/>
    <dbReference type="KEGG" id="tsr:106542729"/>
    <dbReference type="CTD" id="85477"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000004">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000005">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000009">
        <property type="entry name" value="gelsolin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000037">
        <property type="entry name" value="macrophage-capping protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF78">
        <property type="entry name" value="SCINDERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0117">Actin capping</keyword>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="14">
        <location>
            <begin position="28"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="14">
        <location>
            <begin position="148"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="14">
        <location>
            <begin position="268"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="14">
        <location>
            <begin position="412"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="14">
        <location>
            <begin position="527"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="14">
        <location>
            <begin position="629"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013914018.1"/>
        </source>
    </evidence>
    <sequence length="718" mass="80923" checksum="FF3CC326F6FAF8CB" modified="2020-10-07" version="1">MAPPELYHKEFARAGQKVGLQIWRIEKMDLVPVPEPLHGDFYVGDAYLVLHTVKKSNATFYNLHYWLGKECTQDESTAAAIFTVQLDDYLGGKPVQHREIQGYESTQFVGYFKGGIKYKAGGIASGFKHVITNDLSAQRLLHIKGRRVVRATEVPLSWQSFNKGDCFIVDLGTHIYQWCGSTCNKYERLKATQVAVGIRDNERNGRAQLIVVDEGSEPRELLKVLGPKQNLQEGNDNDDEAADITNRRNAKLYMVSDASGSMKITLVAEENPFSRAMLLSEECFILDYGAARQIFVWKGKNANPDERRAAMKSAEEFVQQMNYPLNTQIQILPEGGETPIFKQFFKDWKDKDQSNGFGKVHVMERVAKIASVEFDASKLHESPQMAAQHNMIDDGSGKVEIWRVESNGRIPIEPGSYGQFYGGDCYIVLYTYPKGKIIYTWQGAHATQDELTHSAFLTVQLDRALDGHAVQIRVSQGKEPPHLLSLFKDKPLIIYKDGTSKATGQMPPAATRLFQIRRNFGSITRIVEVDVDAISLNSNDVFVLKLPTKSGYIWIGKGASKEEEKGAKYIAGILKCQTSRIEEGHEPQEFWTALGGKKQYQSSPHLQSESEDHPPRLYGCSNKTGRFIIEEVPGEFTQEDLAEDDVMLLDTWDQVFVWIGNDANETERAESVKSAKNYIETDPSGRDKGTPVVIVKQGFEPPTFTGWFLGWDYNKWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTQ4</accession>
    <name>A0A6I9XTQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Homeodomain-interacting protein kinase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HIPK2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914078.1">
        <property type="nucleotide sequence ID" value="XM_014058603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTQ4"/>
    <dbReference type="GeneID" id="106542780"/>
    <dbReference type="KEGG" id="tsr:106542780"/>
    <dbReference type="CTD" id="28996"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042771">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000022">
        <property type="entry name" value="Homeodomain-interacting protein kinase 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF53">
        <property type="entry name" value="HOMEODOMAIN-INTERACTING PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="202"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="712"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="775"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="776"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914078.1"/>
        </source>
    </evidence>
    <sequence length="978" mass="107046" checksum="D63319D32DE6A827" modified="2020-10-07" version="1">MAPMYEGMASHVQVFSPHTLQSSAFCSVKKLKVEPSSNWDMTGYGTHSKVYSQSKNVQSSQAAAAINASLQIPNPSIPYEQTIIFPASTGHIVVTSANSTSGVVAVSGQALGGPHNLMRRSTVSLLDTYQKCGLKRKSEEIENTSSVQIIEEHPPMIQNNASGATVATTTTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQVGMVGFVAIVNATSTPSQFKHLLGIFTAKDLSWATSVQQLKNQINLLVEHLNKVSHVTKYQEQFNVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLNTVHNQATTLAPTSTTSATISLANPDVSILNYQSALYQPSAASMAAVAQRSVPLQTGTAQICARPDPFQQALIVCPPGFQGLQTSPSKHAGYSVRMENAVPIVTQAAGAPPLQIQPGLLAQVNNFFFLWNLFFDKLIKTLEKKRIWLCFYLSPDRNVLLKSFLLFYSPLSKQRKNVISCVTVHDSPYSDSSGNSPYAVQHRGGNNNGNNYETKVAPESHCNGNPRTIIVPPLKTQASEVLVECDSLVPVTTSHHSSSCKSKSSSIVTSTSGHSSGSSSGAIAYRQQRPGPHFQQQQPLNLSQAQQHLATGSHRRQQAYITPTIAQAPYSFPHNSPSHGTVHPHLAAAAAAHLPTQPHLYTYTAPAALGSTGTVAHLVASQGSARHTVQHTTYPASIVHQVPVSMGPRVLPSPTIHPSQYQAQFAHQTYISASPASVYTGYPLSPTKINQYPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVL2</accession>
    <name>A0A6I9XVL2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC106545920</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106545920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918122.1">
        <property type="nucleotide sequence ID" value="XM_014062647.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47130">
        <property type="entry name" value="SI:DKEY-19B23.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47130:SF5">
        <property type="entry name" value="ZP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013918122.1"/>
        </source>
    </evidence>
    <sequence length="363" mass="41355" checksum="8B7048962AEBD7AA" modified="2020-10-07" version="1">MKLDKSFLQKKFFKIEVIDPSGVPFLVDQMLGARCGYTLSKDVWGNPIFRASFLGCHVINEKDEQFSLTVNIKVSLYEDLRAAAVYQHPMHCYFTWAPREILCEENYMEVSVKTEVPGISDPELMSVLPEAQKVRHQTWNLMFYSSSGTKTTGVRDADKLGYSFNNTLARVFLRSPYSTNETQNTVVNGVTMSTISSTSRYRQRWLLLLIDTTVSCPVDGTSFTDAFLIWTMPMINPRLVLQESTFRSLQVTMGINGQKIENPEKFNYTLEHNITHIVVTIPIGAPRGRLQSTVSNDVYGVTYSIDLLMEHSWTDSDWQLTKYLVIKPTTIPFLPRIPVVINSTKPLELIVPFNSIKLLMSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVU0</accession>
    <name>A0A6I9XVU0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Voltage-dependent calcium channel gamma-like subunit isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMEM37</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Toxicofera</taxon>
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            <taxon>Colubroidea</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
        <source>
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        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920284.1">
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        <property type="component" value="Unplaced"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:voltage-gated monoatomic ion channel activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Tmem37"/>
    </dbReference>
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        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-LIKE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31767:SF0">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-LIKE SUBUNIT"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15108">
        <property type="entry name" value="TMEM37"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920284.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="22268" checksum="3E39DE025DA0A3D4" modified="2020-10-07" version="1">MTAIAAQTQWLLTRRRPKKSFFETFIRSLIILCVAIAIVLLSVAVCDGHWLFTRGKLFGLWHFCTLGSNSTLKCTTDLSLTNIRGISVGMIYVRSMVSFAVVVAIFGLELLMVSQVCEDINSRRKWSMGSVLILVSFLLSSAGMVSFLILLRDYITITSFTLTYWCEFIATFLFFLNGISGLHLNNLTFPWNRVIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVU8</accession>
    <name>A0A6I9XVU8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 222</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMEM222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914768.1">
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008496">
        <property type="entry name" value="TMEM222/RTE1"/>
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    <dbReference type="PANTHER" id="PTHR20921">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF05608">
        <property type="entry name" value="RTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914768.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15399" checksum="228985D3449A0FEB" modified="2020-10-07" version="1">MGICTSSGVIRDFAGPYYVSEDNMAFGKPVKYWKLDPNKVYSCGPNAWDTAVHDASEEYKHRMHNLCCDNCHSHVALALNLMRYDNSTSWNMIKLCFLSLLYGKYVSIGGFVKTWLPFFLLLGIILIVILTLHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVV7</accession>
    <name>A0A6I9XVV7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">cGMP-dependent protein kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PRKG1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
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    </organism>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918252.1">
        <property type="nucleotide sequence ID" value="XM_014062777.1"/>
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    <dbReference type="OrthoDB" id="63267at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF68">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="8">
        <location>
            <begin position="73"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918252.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13951" checksum="E8EFD20C4802B9F0" modified="2020-10-07" version="1">MYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIVPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX23</accession>
    <name>A0A6I9XX23_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calcium-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CABP1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920764.1">
        <property type="nucleotide sequence ID" value="XM_014065289.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106547993"/>
    <dbReference type="KEGG" id="tsr:106547993"/>
    <dbReference type="CTD" id="9478"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000069">
        <property type="entry name" value="calcium-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000037">
        <property type="entry name" value="calcium-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043582">
        <property type="entry name" value="CaBP1/2/4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45917:SF1">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45917">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="197"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013920764.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="25955" checksum="51C8453E6FBAD2DE" modified="2020-10-07" version="1">MGNCVKSPLRSLSRKIHQEEKACYKAVQLSEEGPSAGEGQSQGSLMVLAQNCAVMHNLLGPACIFLRKGFAENRQPDRELRPEEIDELREAFKEFDRDRDGYINCRDLGNCMRTMGYMPTEMELIELSQQINMNLGGHVDFDDFVELMGPKLLAETADMIGVKELRDAFREFDTNGDGEISTNELREAMRKLLGQQVGHRDIEDIIRDVDLNGDGRVDFEEFVRMMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXF5</accession>
    <name>A0A6I9XXF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phospholipase A2 inhibitor and Ly6/PLAUR domain-containing protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106548107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920904.1">
        <property type="nucleotide sequence ID" value="XM_014065429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXF5"/>
    <dbReference type="GeneID" id="106548107"/>
    <dbReference type="KEGG" id="tsr:106548107"/>
    <dbReference type="OrthoDB" id="9042679at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019834">
        <property type="term" value="F:phospholipase A2 inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050918">
        <property type="entry name" value="CNF-like_PLA2_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914:SF9">
        <property type="entry name" value="COILED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0593">Phospholipase A2 inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013920904.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31506" checksum="31DC3448A147B168" modified="2020-10-07" version="1">MFVLISYPLRPSKYKEAPERHCTLVLPISIVPQKLSRRQREKSTEIRCWNIYLCKLKVLHSQKTMKYLIFFTALITTANALVCQCNPCTEATCRDAENCYSSDTEIFTGKDTTGKATIKGCQSNTVEKDICTRGFVIMSSESDFYLMNNVTCCKSGDSCNKNITAPLNKNIPNSKLVCPSCFAKATTCEPKIMKCNDLQKKCFNITGKITKGTTPAQDFTARGCAVENNIFKNGSSLVSGDTTYALEIVTFQDAKSGASQISSCLSFAILLPSVLWLLLDRSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY44</accession>
    <name>A0A6I9XY44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein kinase NIM1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">NIM1 serine/threonine-protein kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">NIM1K</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013921159.1">
        <property type="nucleotide sequence ID" value="XM_014065684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY44"/>
    <dbReference type="GeneID" id="106548332"/>
    <dbReference type="KEGG" id="tsr:106548332"/>
    <dbReference type="CTD" id="167359"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14075">
        <property type="entry name" value="STKc_NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000346">
        <property type="entry name" value="Serine/threonine-protein kinase NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049571">
        <property type="entry name" value="NIM1K_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF49">
        <property type="entry name" value="NIM1 SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="73"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013921159.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="49696" checksum="C79399FECA6B0A46" modified="2020-10-07" version="1">MSMVHCNGDPGSHSNDRWNHEDNGENNANEVLNTETHPGNQHQLTPFEKLILDMSQSEKIVKELTLGKRIGFYSIRGKIGSGNFSQVKLGIHTLTKEKVAIKILDKSKLDYNTKKLLSSEISSMEKLHHPNIIRLYEVMETFSKLYLVMEYANGGELFAKIILDGKLSEEKSKHVFAQIVSAVKHLHENNIIHRDLKAENIFYASNTCVKVGDFGFSTVSQKEEPLNTFCGSPPYAAPELFRDENYIGVYVDIWALGILLYFMTTGFMPFQADTVAKLKKYILGGIYSVPPFLSEPCQRLIWAILQPVPSERWSIDKIMNSEWMNGVQYPKPLEPFKLDPRFLSEDKTLQKEEAEVKIILNHLGITEKHIQNNHGTDYYTSVTGTYRIVLHRVQRKQAMEIVPLVSQPELTKERSKKNLCSYQGHKYSSKLCLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYR1</accession>
    <name>A0A6I9XYR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Dystroglycan 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Dystroglycan</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Dystrophin-associated glycoprotein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31 32 33" type="primary">DAG1</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32 33" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31 32 33">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.</text>
    </comment>
    <comment type="function">
        <text evidence="21">Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="25">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909620.1">
        <property type="nucleotide sequence ID" value="XM_014054145.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909621.1">
        <property type="nucleotide sequence ID" value="XM_014054146.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909622.1">
        <property type="nucleotide sequence ID" value="XM_014054147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYR1"/>
    <dbReference type="GeneID" id="106539361"/>
    <dbReference type="KEGG" id="tsr:106539361"/>
    <dbReference type="CTD" id="1605"/>
    <dbReference type="OrthoDB" id="5990676at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016011">
        <property type="term" value="C:dystroglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016203">
        <property type="term" value="P:muscle attachment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021675">
        <property type="term" value="P:nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11305">
        <property type="entry name" value="alpha_DG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11303">
        <property type="entry name" value="Dystroglycan_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000684">
        <property type="entry name" value="Dystroglycan 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1040:FF:000001">
        <property type="entry name" value="Dystroglycan 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1040">
        <property type="entry name" value="Dystroglycan, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027468">
        <property type="entry name" value="Alpha-dystroglycan_domain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041631">
        <property type="entry name" value="Alpha_DG1_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006644">
        <property type="entry name" value="Cadg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008465">
        <property type="entry name" value="DAG1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030398">
        <property type="entry name" value="SEA_DG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21559:SF22">
        <property type="entry name" value="DYSTROGLYCAN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21559">
        <property type="entry name" value="DYSTROGLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18424">
        <property type="entry name" value="a_DG1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05454">
        <property type="entry name" value="DAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05345">
        <property type="entry name" value="He_PIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00736">
        <property type="entry name" value="CADG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111006">
        <property type="entry name" value="Dystroglycan, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51699">
        <property type="entry name" value="SEA_DG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 28" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <keyword evidence="10 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 27" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636618" description="Dystroglycan 1" evidence="28">
        <location>
            <begin position="28"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="740"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S72" evidence="29">
        <location>
            <begin position="595"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="315"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="397"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="814"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="411"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="26">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="823"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="26">
        <location>
            <begin position="850"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00026224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013909620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_013909621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_013909622.1"/>
        </source>
    </evidence>
    <sequence length="886" mass="97174" checksum="92F9E43331DE5250" modified="2020-10-07" version="1">MNPGFVQLPPVLGRTLLVLTLITSAKCHWPSETSEVLRDWEHQLEASMHLVLSDLRESAPAVVGIPDSSAVVGRTFRVTIPTELIASNGEIIQIAEAGKESLPSWLHWEPESRTLEGLPLETDKGIHYISVTAVHLVSNGSYVPQTTNVFSIEVHPEDHNEPQSVRVALQDVNEASPFVCSAEEPVTILTVILDADLTKMTPKQRIELLNRMKTFSEVELYNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLSQNSVPNISNVESPAKEGTMSAHLGYPVVGWHIANKKPNLPKRIRRQINATPTPVTAVGPPTSAPQEPPSRIVPTPTSPAIAPPTETTAPPVREPIPLPKKPTVTIRTRGPIIQTPTLVPAQPTRTVEVGTITSGQIRPTMPGQGEATAVVTPPTTKKPKGTTPKVSTPSTTDTGTTRRPRPSKTPRPPKPPRTTKPSLVEKTVSPPTRLRTTAVGLPRVPNDPPVLKNHIDRVDAWVGTYFELKIPSDTFYDKEDTTTDKLQLTMKLENEKALEESSWVQFNSTSQLMYGLPNHTHEGRHEFFMHATDKGGLSAVDAFEIFVHTLEDDEIPPVKFKARFHGDHNTVANDINKKIVLVKKLAFALGDRNSSTITLENIGKGSILVEWTNNTLPLDPCPKEKILILSRKISDESGVPSTVFSNTLEPEFKPIDISVVGAGSCKHIQFIPVTKDGFPTTVRPTEIVQRAPEKTSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQNMPETTPLNQDVIGEYTPLWDEDPNAPPYQPPPPFTTPMEGKGSRPKNMTPYRSPPPYVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYZ2</accession>
    <name>A0A6I9XYZ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peroxisomal NADH pyrophosphatase NUDT12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106546676</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_013919081.1">
        <property type="nucleotide sequence ID" value="XM_014063606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYZ2"/>
    <dbReference type="GeneID" id="106546676"/>
    <dbReference type="KEGG" id="tsr:106546676"/>
    <dbReference type="OrthoDB" id="10249612at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000167">
        <property type="entry name" value="peroxisomal NADH pyrophosphatase NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="347"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013919081.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="49800" checksum="E668D3F51AB749B5" modified="2020-10-07" version="1">MEIMEDSEGNPKKQLVSELHQLAADGNKARLTALLDRSPSLINETARNGWTALMYATRNGHFDVVQVLLDKRCDRFRTNTSNQTALDIARFWGYKNIANLLANVEGGLEPFFFTYQVKEPEHYFCRTFLDRKSEKRVDSEWLSMKQKEPTTIYIIFSHLNPLVTSDDDERGFQYPKVKLCKFFYKDIKRYLEQTKTITTIFMGVEPQRINAKDLNTGKEDENGLITWFALRIDATAADSFLQEHPGCYFLHPPMPGLLQLSENEADPVVIMQVIHPEGNHCLLGRKKRFPPGMFTCLAGFIEPAKSLKDGSTRLSRDQVLKDARTNNSSQEEEEPDRWARRRKWFKNSKRHPSARKYSKNSSFTKGSMNSERGCPLDLGPHRESEERGFYTEMFHSASWMFQGDDANADNNLRCLSKRPVTDKYQLMPTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZN8</accession>
    <name>A0A6I9XZN8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Paired box protein Pax-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106547292</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919882.1">
        <property type="nucleotide sequence ID" value="XM_014064407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZN8"/>
    <dbReference type="GeneID" id="106547292"/>
    <dbReference type="KEGG" id="tsr:106547292"/>
    <dbReference type="OrthoDB" id="3225452at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022130">
        <property type="entry name" value="Pax2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF19">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12403">
        <property type="entry name" value="Pax2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired-box protein 2 C-terminal" evidence="8">
        <location>
            <begin position="79"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919882.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="20235" checksum="76E2F62884AFCC2D" modified="2020-10-07" version="1">VTESSVPNGDSQSSVDSLRKHLRADTFTQQQLEALDRVFERPSYPDVFQASEHIKSEQGNEYSLPALTPGLDEVKSSLSTSANPDLGNNVSGPQTYPVVTGRDMASTTLPGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNPYSHPQYTTYNEAWRFSNPALLSSPYYYSATSRGSAPPTAAAAYDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1D6</accession>
    <name>A0A6I9Y1D6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="17">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC106547951</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013920712.1">
        <property type="nucleotide sequence ID" value="XM_014065237.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547951"/>
    <dbReference type="KEGG" id="tsr:106547951"/>
    <dbReference type="OrthoDB" id="65481at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05036">
        <property type="entry name" value="PTKc_ALK_LTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000113">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000117">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055163">
        <property type="entry name" value="ALK/LTK-like_GRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF629">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12810">
        <property type="entry name" value="ALK_LTK_GRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026669605" description="Tyrosine-protein kinase receptor" evidence="20">
        <location>
            <begin position="17"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="852"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="22">
        <location>
            <begin position="68"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="22">
        <location>
            <begin position="286"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="933"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1338"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1338"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1360"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="967"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013920712.1"/>
        </source>
    </evidence>
    <sequence length="1460" mass="160363" checksum="8B1DBBF48B0167A8" modified="2020-10-07" version="1">MDCFLHLIFYFQLVLSVTTLGVASTKSDEKDSSFPYVPPERNWIISSGHSSLPATVSQERNWTRQAETPCNFGWNCSWIMSANSFGQPDWLLSSVVALTSQEDQHIMDIISSNRDLEGDILLLNPSRILDRCTFSLHSPILPWSSSSCLLELAVYSQGLSLGGLSAEVHYVDTNQSKAIHLQEGHEEDVWVKWKILIGLIGQMERPFEITLLYSSCDEKNSGFLALGSLQLQNCLHDKDLYSFDKGSPIPCPHEGCVSYLEVCSFYSDCPTKEEDESLCDSLTEGAHCSFEEGACGWTIDGNINSSWSISDFSRMHENQIGGSALQATGGHFLYLHVNSEHTDGLARTYSTDLPAILANGSYKVQFSAYVFGNYNGTISFTVQEDEGATNGSTALQIWEKSGSWSDHWFLITLEMPSLQNSFHLELLANWGWNSQADIAVDNITFGLDCFSNGRKQSHSDGEQHYSLAIDRLDERLFNPLEEPLLLGETPVNLWWFTACGASGPRGPTQAQCDSAYHNTNMSVTVEKEGHLRGVQIWKVPVSNRYTISAYGASGGKGAKNHNKRSHGIFISANFQLEKDELLYMLVGQQGEDACLRINYHTKQICLGESSEIEEEYRKAKGLTEWAGGGGGGGGATYVFRQKDGAFEPLLVAAGGGGKAYLKAQESALDDAFLEQYVNSSAIPGVNGRTGAAGGGGGWEDITQFPWAGKSLLEGGEGGEACQQALEKLQWATSGGFGGGGGACTSGGGGGGYKGGHASETDDITADGQYGESFVNPMGEIFLSPLAVMESHGEVVIEVYVNCSHCQSGICKQEPGSHLPVCICSAGAILANDNVSCTVIREPIPEEHLPLPFLLTVIAVIVVLVMILTCGSLSIVYHRKKKMLEGTRAHLQNPDYNLSKIRTSTIMTDYNPNYSFAGKTASLSELKEIPRKNISLLQELGHGAFGEVYKGTVVGITGDSSPLQVAIKTLPEISSEQDEMDFLMEALIISKFSHQNIVQCIGVSLQTLPRFILLELMTGGDMKSFLRQNRPRENQPSALTMLDLLNIARDIACGCKYLEENHFIHRDIAARNCLLTCSGSGRVAKIGDFGMARDIYRASYYRKGGRAMLPVKWMPPEAFLEGIFTSKTDTWSFGVLLWEIFSLGYMPYPCKTNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHSPEYRPNFSTILERIKYCTQDPDVIHTELPIECSPIPEDEGSTVLHPNSNTGIVPLLGNQCPQISHQAIEEHDSGNKYGQCPQELLVENLSNWRARRPSLPRFNYSSSGAWAQPVLNQKLDSTSQPAHKLKNKTKNLWNPTYGSWVMENNCQSSPSSKLNTMQEREDSGFDGTYSPSPTSRASPSSPSQNHSPHIDIGGIELVKLQNFPCGNVNYAYDDLCYEIGHPSPLAVEPATVIRSGSLHRDSKFVFKTEKTSRERDSGLSLSDDLNVTPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y337</accession>
    <name>A0A6I9Y337_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Complement C4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106548324</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921146.1">
        <property type="nucleotide sequence ID" value="XM_014065671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y337"/>
    <dbReference type="GeneID" id="106548324"/>
    <dbReference type="KEGG" id="tsr:106548324"/>
    <dbReference type="OrthoDB" id="6359008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006956">
        <property type="term" value="P:complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00017">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.91.20">
        <property type="entry name" value="Anaphylotoxins (complement system)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000020">
        <property type="entry name" value="Anaphylatoxin/fibulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018081">
        <property type="entry name" value="Anaphylatoxin_comp_syst"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001840">
        <property type="entry name" value="Anaphylatoxn_comp_syst_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF86">
        <property type="entry name" value="COMPLEMENT C4-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01821">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00004">
        <property type="entry name" value="ANAPHYLATOXN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00104">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47686">
        <property type="entry name" value="Anaphylotoxins (complement system)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01177">
        <property type="entry name" value="ANAPHYLATOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01178">
        <property type="entry name" value="ANAPHYLATOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026983191" evidence="4">
        <location>
            <begin position="26"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphylatoxin-like" evidence="5">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921146.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17749" checksum="C81DEF94BA4A5201" modified="2020-10-07" version="1">MKSVSLLFLRLTSTNILSSLPLSSAVSKYQTPNQVKCCKGGIILLRNARSCEERAKRIQNTECREVFLDCCRYAAKLRRQSWGSHGLARVDDEDEEDYFDDDFITLRRFFPESWLWKTFVVDKTLTELFHLPDSITTWEIQAVSVSLAKGKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3A4</accession>
    <name>A0A6I9Y3A4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106548419</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921261.1">
        <property type="nucleotide sequence ID" value="XM_014065786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3A4"/>
    <dbReference type="GeneID" id="106548419"/>
    <dbReference type="KEGG" id="tsr:106548419"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="100"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921261.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="41602" checksum="83288BFCA1391F18" modified="2020-10-07" version="1">MDSQETLFTIDEEDIIWPRRFNQVQPQSLCNSKCQSGYARSKIEGKPFCCYDCLKCPYGKISNQENMNECFQCPEDQYPNKKQDFCLPKVITFLKYEESLGTILASSALFFALVTAAILWIFIKHQDTPIVKANNRNLTYTLLVGLMLSFLCSLLFLGRPGKVTCLLRQTAFGMIFSMAISCVLAKTIIVVLAFTATKPGSMMSRWVGKRLVIFIILGCCCIQGIISITWVTTSPPFPDADMNSVVEEIILECNEGSAFMFYCILGYMGLLAIISFTMAFLARNLPDVFNEAKFITFSMLVFCSVWLSFVPTYLSTKGKYMVAVEIFSIISSSVGLLVCIFFPKCFIIVLRPALNNKKQLRRQTYRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4U1</accession>
    <name>A0A6I9Y4U1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Eomesodermin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">EOMES</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912565.1">
        <property type="nucleotide sequence ID" value="XM_014057090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4U1"/>
    <dbReference type="GeneID" id="106541592"/>
    <dbReference type="KEGG" id="tsr:106541592"/>
    <dbReference type="CTD" id="8320"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002302">
        <property type="term" value="P:CD8-positive, alpha-beta T cell differentiation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001714">
        <property type="term" value="P:endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20205">
        <property type="entry name" value="T-box_TBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000004">
        <property type="entry name" value="T-box, brain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032385">
        <property type="entry name" value="T-box_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF13">
        <property type="entry name" value="EOMESODERMIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16176">
        <property type="entry name" value="T-box_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="245"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="638"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013912565.1"/>
        </source>
    </evidence>
    <sequence length="678" mass="73362" checksum="DF0A3D408E550559" modified="2020-10-07" version="1">MQLGEQLLGTPANLPGAPFYPLEAAGGRAGATARHLNTGSPPRLNLEKVPKKFGGASVPAASLLGEAEAAGETPPFVTAGQMPSKAVTEVADGGRKSSPCPTEEDVLPPPPPPPPARYSLDGLSPERYYLQSPGPQANAELGSPCSLFPYAGTPGSAQSSSMYQAPSGARYPYGSVLTPPGSFSTTAGRTPFAAAASTYQYGQGAPPGALYNPYPPAAAAGGSCSGLGGLAMPGGAGGLRAQVFLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNITGLNPTAHYNVFVEVVLADPNHWRFQGGKWVTCGKADNNMQGNKVYVHPESPNTGAHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDMNDSTKTQTFNFPETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTASENDRLTPSPTDSPRSHQIVPGARYTVQPFFQEQFVNNLPPARFYNGERAVPQTNGLLSPQQAEEVTNPPQRWFVTPVQQSGANKLDMNSYETDYSPSSLLSYGIKSLPIQTSHALGYYPDPTFSSVAGWGSRGSYPRKMSTGLPWTSRTSPPGFSEDLLSKEKVKEEIGSSWIETPPSIKSLDSNDSGVYIGACKRRRLSPNSSSNENSPTMKCEDINADDYSKDASKGMGYYAFYTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4V2</accession>
    <name>A0A6I9Y4V2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="4">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ABCG2</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>pheophorbide a(in) + ATP + H2O = pheophorbide a(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61360"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58687"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:61361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>sphing-4-enine 1-phosphate(in) + ATP + H2O = sphing-4-enine 1-phosphate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38951"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60119"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:38952"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013919258.1">
        <property type="nucleotide sequence ID" value="XM_014063783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4V2"/>
    <dbReference type="GeneID" id="106546816"/>
    <dbReference type="KEGG" id="tsr:106546816"/>
    <dbReference type="CTD" id="9429"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF92">
        <property type="entry name" value="BROAD SUBSTRATE SPECIFICITY ATP-BINDING CASSETTE TRANSPORTER ABCG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter family G" evidence="22">
        <location>
            <begin position="107"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="21">
        <location>
            <begin position="238"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF19055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013919258.1"/>
        </source>
    </evidence>
    <sequence length="521" mass="57719" checksum="9FA7FC0B3F397DDD" modified="2020-10-07" version="1">MGAIFFGLTNDGKGLQNRIGAMFFMTTNQCFSSISAIELFVVGTYFARGVSGGERKRTSIGLELITNPKVLFLDEPTTGLDSSTANAVLLLLKRMSLQGKTIIFSIHQPRYSIFKLFDSLTLLAAGRLLYHGPADNALPYFKSLGFVCEPYNNPADFFLDIINGDSSAVAASKMTIHSAILEATEREQETIAVTLAVQFSKCSFYKKMLEELDVLSKEQKRLGTIIKGIPYSTPFLHQLKWLSRRTFKNLIGNPQNSTAQIAIITVMGIITGAVFSGVKNDATGIQNRIGALFFLATNQVLNSITTVELFITEKRIFIHEYISGYYRVSAYFFSKIIADMMPMRTLPGIILTSLNYFTIGFKPVLASFFIMMLTIILIAYASSSLALAVATGQNVTSVANLLINICFVFMIVFSGVLVNLTTITSSLSWLEYFSIPRYGLNALQINEFTGLRFCTETPVGVNATCVSTSAYTVCTGEDYLEMQGIGTTSWDLWKNHVALICMTITFLTFAYLKLKFLKKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y660</accession>
    <name>A0A6I9Y660_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcriptional adapter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transcriptional adapter 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TADA1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TADA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920413.1">
        <property type="nucleotide sequence ID" value="XM_014064938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y660"/>
    <dbReference type="GeneID" id="106547702"/>
    <dbReference type="KEGG" id="tsr:106547702"/>
    <dbReference type="CTD" id="117143"/>
    <dbReference type="OrthoDB" id="10264870at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22934">
        <property type="entry name" value="HFD_TADA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024738">
        <property type="entry name" value="Hfi1/Tada1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12767">
        <property type="entry name" value="SAGA-Tad1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013920413.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="37734" checksum="9E35FD6A0EB25423" modified="2020-10-07" version="1">MATYVSELEVAKKNLSEALGENVKQYWANLKLWFKQKISKEEFDLEARRLLTQDNVHFHNDFLLAILTRCQILVSAPEGAGSLPWTGGSAPKPGKPKGKKKFSSVRQKFDHRFQPQNPLSGAQQFVAKDPQEDDDLKLCSHTMMLPTWGQLEGRMIVTAFEHGLDNVTEEAVTVVVYAVEKHLKDILTSVISRRKAYRVRDGHFKYAFGSNVNPQPYLKNSVIAYNNLTECPPTCLAPPPGQNLASHLPPDDAEQQAALLLACSGDTSPATLPPVNMYDLFEALQIHREVVPAHTVYALNIERIILKLWHPNHEELQQDKIHRQRLAAKEGLLMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7S2</accession>
    <name>A0A6I9Y7S2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitochondrial import inner membrane translocase subunit TIM44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TIMM44</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim44 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915670.1">
        <property type="nucleotide sequence ID" value="XM_014060195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7S2"/>
    <dbReference type="GeneID" id="106544053"/>
    <dbReference type="KEGG" id="tsr:106544053"/>
    <dbReference type="CTD" id="10469"/>
    <dbReference type="OrthoDB" id="10265990at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tim44-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013915670.1"/>
        </source>
    </evidence>
    <sequence length="74" mass="8422" checksum="7E3C18AF6D7734B0" modified="2020-10-07" version="1">MGKMMEQGPVLIITFQAQLVMVVKNQKGEVVEGDPEKVLRMLYVWALCRDPEELNPYAAWRLLDISASSTEQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y846</accession>
    <name>A0A6I9Y846_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein Rlf</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RLF</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916290.1">
        <property type="nucleotide sequence ID" value="XM_014060815.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106544523"/>
    <dbReference type="KEGG" id="tsr:106544523"/>
    <dbReference type="CTD" id="6018"/>
    <dbReference type="OrthoDB" id="8691423at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052251">
        <property type="entry name" value="GH-ZnFinger_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057986">
        <property type="entry name" value="TPR_Rlf/292/654"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058902">
        <property type="entry name" value="zf_C2H2_ZNF292/Rlf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15507">
        <property type="entry name" value="ZINC FINGER PROTEIN RLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15507:SF18">
        <property type="entry name" value="ZINC FINGER PROTEIN RLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25580">
        <property type="entry name" value="TPR_Rlf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26218">
        <property type="entry name" value="zf_C2H2_ZNF292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="451"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="494"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="551"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="581"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="739"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="942"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1090"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1142"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="632"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="763"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="860"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1026"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1273"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1375"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1436"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1482"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1533"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="644"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="763"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="779"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="872"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1030"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1273"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1495"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1536"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1566"/>
            <end position="1577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013916290.1"/>
        </source>
    </evidence>
    <sequence length="1677" mass="190403" checksum="54D65BDC1ADE3616" modified="2020-10-07" version="1">MIQKAGPVIPTLLQYAGDQGTSEQVLPFLEVYRVAIQSFASARPYLTTECEDVLLVLGRLVMSCFEQLLSVPESEELSEPFLRLCQSIQASILTVLSXGDNSLGILADISKEGVWKNPVLLKILSQQSVEAEEMNQLIKREGLAFLQMRVKHLMKTNRLPQAASLSKLCKESTEIPDVSPFLQSYITCLCSMLPNEESIKEISKVDCKEVLDMICNLEAEGQDSTAFILCTTYFTQQLQTASVYCSWELTLFWSKLQRRIDPSLDSFLERCRQFGILARTLQHLFFLIRVIQAEAEEAGLAVSILLCVRALQLRSNENDDMKSSVCKTIACLLPEDLEVRRACQLTEYLLEPSEAGYSLLEELHVQPDQKFDEENAPVPNSLRCELLLALKEKKLLQKKRWLAAQSNLSRITLDGNRPLKIIHCEKPRSDPQDYVTFSKLENCHLQDQDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNNDENAMHYLDMKNRREKCSYCRRHFMSAFHLHEHEQVHIGPLPYMCVSVGCYARFGSVNELLHHKQQHDDLRYKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCSFRGCKKFYYSKTELTNHLSVHMSNGEMKEASIKHEGNVSQDGLACPLDSEALENKGPPEQQENLSLPSGSTEREGMLGVKQEPLSDGEEGGQNNGSLENHASSLANENQVSSVTDTVDRGQAFSGAVLPQERACLPSNLKERYSNVAVYFDGKKFTCGFEGCGSTYKNSKGMQKHLRRVHPYHFKPRRKDKDISQLESPAGENSSDSQDVADFSDVCKSRSCFKEELCPSSPESSFYVHSKMSSTEDSMFELLLGLKHLSLKNSSGHTSPRFLQSYSSKCPNSEDESTSDHFTEEQRSKLPTQYLTQLAAKPFFCERQGCTCEFVTREALLTHYVRKHNYSKEMVLQLNMFQHRYSPFKCHICQRSFTRKTHLRVHYRNKHKLGNMSVSQKLFANESFEDLNECGEDRLNSTEDSMSAFYTSTDGELDSKTKLAVDQHCHSKEDFSSDSDLDSSEEAESCIPGKQAKLSSLDSHRKELEANEGRGSKRTVAKGNLCYILHKYHKPFHCIHRNCNSAFTNQKGLIRHYRTVHRYNKEQLCLEKDKERTKREFAKCKKLFVCKHKACSKRFLCSKSLAKHCVDSHAFDPEDDSKVLSETVKFPCHHPHCPAAFYSFHKLEHHLMEEHEKEDLNKGIEIHCDLNGCNRVFTTYSSYSQHVYFRHSDYDGVLGGAKEEEQRCLKYRCLRQDGSEKKRQNSEKGERGGSRNKGKQLYAFRTRVEALQMCRDNCNQTQYPCMVRGCPSVVKLESSIVRHYKRTHQMAGAYIEQQYDELVLCVDCGTALNDLCMEANSEPEGDSDFTPDVTGHPANEHKRERHLPNSSCDEKGGQLGRYSEGEESQDALVYSGTLKHIHPHPKTRCSEDCDRGGSSQQSEAGSILRNHGQENASYCAKSNLPLSREKDAGDWQSSSQEETKKLPFAVPKEKAQKPFDLKSFKPMGFESSFLKFIQESQEREEEEEDDEDDDLENEWQPVGPYGMGGGIEKAKDSRREVSQDRGLYTNVPPAAISKKNSGTKAHGHLRGMQPLLSADSPSLPSLENLRAILGKALTGCGDHALKQLHYLRPVVVLERSKFSPSLIDFFPTKKTDHLCVGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8D5</accession>
    <name>A0A6I9Y8D5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLC6A5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916735.1">
        <property type="nucleotide sequence ID" value="XM_014061260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8D5"/>
    <dbReference type="GeneID" id="106544859"/>
    <dbReference type="KEGG" id="tsr:106544859"/>
    <dbReference type="CTD" id="9152"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF241">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GLYCINE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013916735.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="46602" checksum="2F48B5EEB6E8456D" modified="2020-10-07" version="1">MVQIRLREHVERMAVPFPILLSQESAEYIVSELRRTDLPQLHENREQYFSDKDHHDYAYPKEMSEQLVQSPEVVAPGPLERAGIHRNSPETEQPQNFQSQVPPQLAKVGTALSGDIQPWPLDEGPPHETERAKIGIGKVASAQGNPPTGKDPADLCTLKNAPCAGNAAPANIVPCKIAPLQSAVGPANQSLGKSLVEPNNTNTGWVNMSHSTVVLGTDGIASVRPGSVTEEDEEGDENKARGNWSSKMDFILSMVGYAVGLGNVWRFPYLAFKNGGGAFLIPYLTMLALAGIPIFLLEVSLGQFASQGPVSVWKAIPAFQGCGIAMLIISVLIAIYYNIILCYTLFYLFASLSPVLPWASCNNPWNTPDCRDKNKLLLDSCIIGDHPTLQIKNSTSCMSAYPNLTMVNYTSHVSNKTFVSGSEEYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y970</accession>
    <name>A0A6I9Y970_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.143</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Beta-1,2-N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">GlcNAc-T II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Mannoside acetylglucosaminyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">MGAT2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12941"/>
            <dbReference type="Rhea" id="RHEA-COMP:13526"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60651"/>
            <dbReference type="EC" id="2.4.1.143"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 16 (GT16) protein family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.143" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013918160.1">
        <property type="nucleotide sequence ID" value="XM_014062685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y970"/>
    <dbReference type="GeneID" id="106545954"/>
    <dbReference type="KEGG" id="tsr:106545954"/>
    <dbReference type="CTD" id="4247"/>
    <dbReference type="OrthoDB" id="6019616at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008455">
        <property type="term" value="F:alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007754">
        <property type="entry name" value="GlcNAc_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871:SF0">
        <property type="entry name" value="ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871">
        <property type="entry name" value="BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05060">
        <property type="entry name" value="MGAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16 26" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="33"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="135"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="241"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="26">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="295"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="352"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="391"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013918160.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="51715" checksum="87ADEA9290BB975C" modified="2020-10-07" version="1">MRLRIYKRKVVALLLVLGAAACALVLWGGTSEAGAGGSSSSSKREEAGSPPPLPGDQPLPPAVTSSSSSLPGNNGRHANSSSVSREEEAPPGLNRTLHYRSLVYRLNFDQPVRNEARWRPGGAGSGEVAVALVVQVHERAEHLRILLDSLRRAPGVEKVLLVLSHDVWSAELNALAASVDFCAVLQIFFPFSLQLYPGEFPGTDPRDCPRDVGQAAALRSGCLNAHYPDAFGHYRESSFTQTKHHWWWKLHFVWERVRTLREHPGLVVLLEEDHYLAPDFYHVLQRLWALRQRDCPECQLLSLGTYATVRGSFAGRADKVELKTWKSTEHNMGMVFARDTYQQLIACTDAFCTYDDYNWDWTLQHLTVGCLPHFWKVLVPEIPRIFHTGDCGMHHKKSCQPSVQSAKIDSLLNSNQQYLFPETVTISKRYSMTPLSPHVKNGGWGDIRDHELCKSYRRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9K2</accession>
    <name>A0A6I9Y9K2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein Dos</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CBARP</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918820.1">
        <property type="nucleotide sequence ID" value="XM_014063345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9K2"/>
    <dbReference type="GeneID" id="106546469"/>
    <dbReference type="KEGG" id="tsr:106546469"/>
    <dbReference type="CTD" id="255057"/>
    <dbReference type="OrthoDB" id="6247020at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045955">
        <property type="term" value="P:negative regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037658">
        <property type="entry name" value="CBARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28597">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT-ASSOCIATED REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28597:SF1">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT-ASSOCIATED REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="679"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918820.1"/>
        </source>
    </evidence>
    <sequence length="750" mass="81551" checksum="588DCB36601C2737" modified="2020-10-07" version="1">MSDAFTSWANTTTEGVTTVPGEVSPQDGYVLLLVLLSIFVGGTLVVLFGVLIVCRRCCGTDRRHTRASDDPEKTNTTYLDDSQPAQDIIIKVDDPECASSSSYRDAESERFLSSGSATGRRVSFNEAALFEQSKKTQEKGRRYTLTEGDFHHLKNARLSHLPLPPSAALKIVTIHECEANENSVMMPPRLPPVKPSLAIFQPTGSPLPKTPLTSHGGLCSSSTLPGDTFNSSVEAASSVSSDSGEGVASGSAAKAAKGAATAPPQEPTSAPPQGTVLHFLTRLRRHASLEGASPYFRIKKWKLETNQRASSLDTRGSPKRHQFQRQRAASESMDQDRDAHQSDIIQYIARTDDVVFRPVAGPFLPAPASPTLSLGRLESSEPPSPTPPEPLEQQPTPLYHDIWSLRASLEKCVSSEKGNDQDSAPSDGGDSISSSGGLPSFASSLADEAEAGEDRSLAKPKQDSVELEAGTRKLLQMDSGYASIEAPSGRVGEDQTASEKRFCFTSAGRKATIFESFEGQAPEAEEEEEEEEEEEEEEAAGAVGGLLPSSPLLWAPYGQMFPARESFPRRDYSIDEKTDALFNAFVRHDPQFDESPLRAKHRSRTHLRKQWQRTKQYSDPGVRYPTALERHRTPLRRGDSVNYPLEGRYHSTLPRIASSGDEEVASPATETGEKIQVIEEEPPEEAKTGPDPYTRDCLGGGQCPGLGTELLDKIAGGLEDMFFTPLKKPRESLECVVMVAARASPEHSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAU0</accession>
    <name>A0A6I9YAU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinase</fullName>
            <ecNumber evidence="7">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106548201</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 1,4,5-trisphosphate + ATP = 1D-myo-inositol 1,3,4,5-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11020"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.127"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:11021"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013921010.1">
        <property type="nucleotide sequence ID" value="XM_014065535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAU0"/>
    <dbReference type="GeneID" id="106548201"/>
    <dbReference type="KEGG" id="tsr:106548201"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.160:FF:000001">
        <property type="entry name" value="Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF55">
        <property type="entry name" value="INOSITOL-TRISPHOSPHATE 3-KINASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013921010.1"/>
        </source>
    </evidence>
    <sequence length="448" mass="50574" checksum="95A46A9C4535FA8F" modified="2020-10-07" version="1">MEPREAQDGSGGSSPELGRRRQLSVGELCSLFEARCAMASRQLPEPARKSCRPRNGVLQTVIPQLTVTSEEDGGEAQRSPAMLPPTEPNGAWLTTDSSLHLLQSSRRLSTSSLSSTGSSSLFEDSEDDLLSDTEGRSQGIVHLEHGEETHQKKAWQTIKTIVNLPVISPFKKRYSWVQLAGHTGSFKAADAGTILKRHCENEEKCFELLMKDSLHSCVPVFHGVVERDGESYLQLDDLLANFEGPCVMDCKMGVRTYLEEELTKAREKPKLRKDMYKKMIDVDPLAPTDEENTQHAVTKPRYMQWRETISSSASLGFRIEGIKKADGTCNTNFKTTKTREQVFQVFEEFIKGNTTILKKYLRRLCEIRGILETSVFFKQHEVIGSSLLFVHDESEHANVWLIDFGKTTFLPDGQILDHRIAWQEGNREDGYLFGLDNLIDILESMLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBA9</accession>
    <name>A0A6I9YBA9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP-binding cassette sub-family F member 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548523</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921385.1">
        <property type="nucleotide sequence ID" value="XM_014065910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBA9"/>
    <dbReference type="GeneID" id="106548523"/>
    <dbReference type="KEGG" id="tsr:106548523"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001197">
        <property type="entry name" value="Putative ATP-binding cassette family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="65"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921385.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="20954" checksum="B1769BD147FFF616" modified="2020-10-07" version="1">MGAAHYSCSRIPGRVICQMINPSDVWQFVYLILQAKEIRAGHNSCFRHGFHVALSAPCNLGKSEEEYRHQLGCYGVSGELAVRPVASLSGGQKSRVAFAQMTMPCPNFYILDEPTNHLDMETIEALAKALNKFRGGLILVSHDERFIRLVCKELWVCGNGTVQRIEGGFDEYRDILQEQFRKEGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCC4</accession>
    <name>A0A6I9YCC4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger SWIM domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ZSWIM5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921777.1">
        <property type="nucleotide sequence ID" value="XM_014066302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCC4"/>
    <dbReference type="GeneID" id="106548831"/>
    <dbReference type="KEGG" id="tsr:106548831"/>
    <dbReference type="CTD" id="57643"/>
    <dbReference type="OrthoDB" id="10013584at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048370">
        <property type="entry name" value="ZSWIM4-8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22619">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN CONTAINING PROTEIN 4, 5, 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22619:SF2">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21055">
        <property type="entry name" value="ZSWIM4-8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="6">
        <location>
            <begin position="246"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921777.1"/>
        </source>
    </evidence>
    <sequence length="1211" mass="133085" checksum="9310C5FCF27D6A0A" modified="2020-10-07" version="1">MAEGGEEEELLRPPLPAAGSPGPKRLCLRAVGAATLPRSGVYPGGPVGTAAAAGGGDGGTRGCCPPGGAGTGPGCGPEGALLPLLQPESLLDAAAKRVAGSWAFERVEGRFQRIPEPVQRRIVYWSFPRSERQICMYSSFQAGRPSGNGASGSASGAAGGGGGGTNLGVGGEENPATVPGLAVPSGASGPAPLPAAASTAISEGLPFRRGIRLLETGAVDNVLQVGFHLSGTVTEAATATEPEMTYKVAISFDRCKITSVTCDCGNKDIFYCAHVVALSLYRIRKPDQVKLRLPISETLFQMNRDQLQKFVQYLITAHHTEVLPTAQKLADEILSSNSEINQVHGAPDPTAGASIDDENCWHLDEEQVREQVKLFLSQGGYYGSGKQLNSMFAKVREMLRMRDSNGARMLTLITEQFMADPRLALWRQQGTGMTDKCRQLWDELGALWVCVVLNPHCTLEEKACWLRQLRKWGDMDVCPLEDGNYSNELPNITSALTQSSSHRQSPLTRPRRTVFTRAIEGCDLHWQDSHLQRIISSDFYISPSYPREGEGLLFNSQGQPLWLEHVPTACARVDALRSHGYPKEALRLTVAIINTLRLQQQRQLEIYKHQKKELLQRGATTITNLESWVGHPLNPIGCLFLTLTEACRIEDENSLDISDASESKPPVYQHVPVANNCPESGESYLSLALEVALMGMGQQRVMPEGLYAQDKVWRNEEQIIACLQELELDAVLVQTLRKQCILLLEGGPFSGLGEVIHRESVPMHTFAKYLFSALLPHDADLAYKLALRSMRLPVLETTPSGDVTHPHHIVSVVPSRYPRWFTLGHLESQQCELASTMLTAAKGDMLRLRTVLEAIQKNIHSSSLIFKLAQDAFKIATPADSNADTTLLNVALELGLQVMRMTLSTLNWRRREMVRWLVTCATEVGVRALVSILQSWYSLFTPTEATSIVATTVMSHSTILRLSLDYPQREELASCARTLALQCAMKDPQNCALSALTLCEKDHIAFETAYQIVIDAASTGMTYSQLFTIARYMEHRGYPLRAFKLASLAMTHLNLAYNQDTHPAINDVLWACALSHSLGKNELAAIIPLVVKSVHCATVLSDILRRCTMTAPGLAGIPGRRNSGKLMSTDKAPLRQLLDATINAYINTTHSRLTHISPRHYGEFIEFLSKARETFLLAQDGHIQFAQFIDNLKQIYKGKKKLMLLVRERFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCD6</accession>
    <name>A0A6I9YCD6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor E2F3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">E2F3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921811.1">
        <property type="nucleotide sequence ID" value="XM_014066336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCD6"/>
    <dbReference type="GeneID" id="106548848"/>
    <dbReference type="KEGG" id="tsr:106548848"/>
    <dbReference type="CTD" id="1871"/>
    <dbReference type="OrthoDB" id="1743261at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14660">
        <property type="entry name" value="E2F_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000008">
        <property type="entry name" value="E2F transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015633">
        <property type="entry name" value="E2F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032198">
        <property type="entry name" value="E2F_CC-MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081:SF44">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16421">
        <property type="entry name" value="E2F_CC-MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="7">
        <location>
            <begin position="52"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="235"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013921811.1"/>
        </source>
    </evidence>
    <sequence length="339" mass="37939" checksum="7263FA8E7C996AB9" modified="2020-10-07" version="1">MPLQQAKRRLELEEPGQQFLPEGLRTPKGKGRAATRSPESPKTPKSPSEKTRYDTSLGLLTKKFIQLLSQSPDGVVDLNKAAEVLKVQKRRIYDITNVLEGIHLIKKKSKNNIQWMGCSLSDFGGMLAHCQGLSKEVAELSQEEKKLDELIQSCSHDLKLLKDSEDRKLAYVKYEDIQEIRSLKDQTVILVKAPPETKLEMLDPEKNKLIHLCSTQGPIEVYLCPEETDYSTRIKTQDQDHNGNISRNISKDLNSDNSGGPDCSENATAISPLVSTTNLLQQTEDQITSSLDGPFVNLLPPLLHEDYLLGLDEEEGISDLFDTYGFEKVPPLMDGFLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCU5</accession>
    <name>A0A6I9YCU5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">WNT2B</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921860.1">
        <property type="nucleotide sequence ID" value="XM_014066385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCU5"/>
    <dbReference type="GeneID" id="106548888"/>
    <dbReference type="KEGG" id="tsr:106548888"/>
    <dbReference type="CTD" id="7482"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19346">
        <property type="entry name" value="Wnt_Wnt2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009140">
        <property type="entry name" value="Wnt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF93">
        <property type="entry name" value="PROTEIN WNT-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01842">
        <property type="entry name" value="WNT2PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921860.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="42963" checksum="F3C7D5C13B920B7C" modified="2020-10-07" version="1">MLGSNRLASSASIASAPEATRRASQGRPVRSRLALPLLLTLLILTPRVDSSWWYIGALGTRVICDNIPGLVNKQRHLCQSYPDIMQSIGEGAKEWIRECQHQFRHHRWNCSTLDRDHTVFGRVMLRSSREAAFVYAISSAGVVYAITRACSQGELKACNCDPHKRGRSKDDRGDFDWGGCSDNINYGIKFAKDFVDAKEKTVKDARALMNLHNNRCGRTAVKRFLKLECKCHGVSGSCTLRTCWLAMSDFRKTGDYLRKKYNGAIQVTMNQDGSGFTVANKNFRKPTKTDLVYFENSPDYCVMDKPAGSLGTAGRVCNKVSRGTDGCEVMCCGRGYDTTQVTRVTKCECKFHWCCAVRCKECEDTIDIHTCKAPKRAEWIDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCU7</accession>
    <name>A0A6I9YCU7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">POU domain class 2-associating factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">POU2AF1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921973.1">
        <property type="nucleotide sequence ID" value="XM_014066498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCU7"/>
    <dbReference type="GeneID" id="106548994"/>
    <dbReference type="KEGG" id="tsr:106548994"/>
    <dbReference type="CTD" id="5450"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070974">
        <property type="term" value="F:POU domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047571">
        <property type="entry name" value="OCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015389">
        <property type="entry name" value="PD-C2-AF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15363">
        <property type="entry name" value="POU DOMAIN CLASS 2-ASSOCIATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15363:SF3">
        <property type="entry name" value="POU DOMAIN CLASS 2-ASSOCIATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09310">
        <property type="entry name" value="PD-C2-AF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52003">
        <property type="entry name" value="OCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCA" evidence="5">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921973.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="25567" checksum="AF3A10F7F117E537" modified="2020-10-07" version="1">MHWQKSSAAEQPLPSRPYQGVRVKEPVKELLKRKRGRAQNAKAAPESMAVFFPHQSLPSYSPTGQVYSETDLVTSSLPLMEEGALYSGWLTQPPPATLQPLTQWTTAPDYVSHEAVSCPYTADMFVQPVCPSYTLVGTSSVLTYTSQPLITNFAARSASSPAVVPHLDAMDQQAPLSYFPWTQPIPTLPATSLQYQPASMALTAPQFVPLPISGVDPLPQVLEDTRKEMAPLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDC0</accession>
    <name>A0A6I9YDC0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ankyrin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106549105</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922142.1">
        <property type="nucleotide sequence ID" value="XM_014066667.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549105"/>
    <dbReference type="KEGG" id="tsr:106549105"/>
    <dbReference type="OrthoDB" id="9019272at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000003">
        <property type="entry name" value="Ankyrin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000001">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000002">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2660:FF:000001">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000915">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040745">
        <property type="entry name" value="Ankyrin_UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF74">
        <property type="entry name" value="ANKYRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17809">
        <property type="entry name" value="UPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="73"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="341"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="374"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="407"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="440"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="473"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="506"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="539"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="572"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="605"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="638"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="9">
        <location>
            <begin position="834"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="9">
        <location>
            <begin position="991"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922142.1"/>
        </source>
    </evidence>
    <sequence length="1367" mass="148732" checksum="E92999EC2745DBA1" modified="2020-10-07" version="1">MAHAASQLKKNRDLELNPEEENEKKRKHRKRSKDRKKKTDTNASYLRAARAGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHIEVVSELIQRGANVDAATKKGNTALHIASLAGQTEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDHGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKMMVNRTTESGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNTNMVKLLLDRGAKIDAKTRSGLTPIHVAAFMGHVNIVSQLMHHGASPNSTNVRGETALHMAARAGQSEVVRSLVQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASANAATTSGYTPLHLSAREGHEDVASILLDHGASLSIITKKGFTPLHVAAKYGKIEVANLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHASAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIAPVHLASQEGHMDMVSLLLTRNANVNLSNKSGLTPLHLAAQEDRINVAEVLVNQGAVVDAPTKMGYTPLHVGCHYGNIKIVNFLLQQFAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGASPNELTVNGNTALAIAKRLGYISVVDTLKIVTEETMTTITVTEKHKMNVPETMNEVLDMSDDEGEDAMTGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASSDRSYTLNRSSYTRDSVMIEELLVPPKDTPPALPREVDSDSLRHYSWAAETLDNVNLVSSPIHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRAKLASPPPMVEGEGLASRLIEMGPSGAQFLGPVVVEIPHFGSMRGKERELIVLRSENGETWKEHQYDSKHENLLEILNGMNEELDSVEELEKKRICRIVTKDFPQYFAVVSRIKQESNQIGPEGGILSSTTLPRVQAAFPEGALTKRIRVGLQAQPVQDEMVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPSGEGVTNGYKGDTTPSLRLLCSITGGTSPAQWEDITGTTPLTFVNDCVSFTTNVSARFWLADCHQVLETVGLATQLYRELICVPYMAKFVIFAKTNDAVESNLRCFCMTDDKVDKTLEQQENFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYAFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKVQPTGQLNIPGSSIKELVCAAKRNDCLALGLSWPLYVLTVFVYAESKSVENIPSLKCPAPSDSELKNFGLGVFCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDJ8</accession>
    <name>A0A6I9YDJ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Copine-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Copine III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">RBM12</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Calcium-dependent phospholipid-binding protein that plays a role in ERBB2-mediated tumor cell migration in response to growth factor heregulin stimulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Monomer. Interacts with ERBB2 (preferentially with the tyrosine phosphorylated form); this interaction occurs at the cell membrane and is increased in a growth factor heregulin-dependent manner. Interacts with SHC1; this interaction may mediate the binding of CPNE3 with ERBB2. Interacts with RACK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copine family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922095.1">
        <property type="nucleotide sequence ID" value="XM_014066620.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549066"/>
    <dbReference type="CTD" id="10137"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 20" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="301"/>
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    <sequence length="1413" mass="152858" checksum="59E3A574B1119A51" modified="2020-10-07" version="1">MAVVIRLQGLPIVAGTMDIRHFFSGLTIPDGGVHIVGGEMGEAFIVFATDEDARLGMMRTGGTIKGSKVTLLLSSKTEMQNMIELSRRRFETANLDIPPANASRSVPPPSSGISSRVTLSTTVSNFNSPSPSVVTAATSVHESNKNIPTFSTASMGTAPPNLGVTFGSPTFSSTISSTASPMNTVPPPPIPPIPALPSLPPMPSIPPIPVPPPVPTLPPVPPVPPIPPVPPVPPITSLPPLSGMPPMNPPPVAPLPAGMNGSGTAMNLNSNLNPMFMNPINAIQINSQSSVKPLPINPDDLYVSVHGMPFSATESDVKDFFHGLRVDAVHILKDHVGRNNGNGLVKFCSPQDTFEALKRNRMLMIQRYVEVSPATERQWVSAGGHITFKQTMGPSGQNLPPQNLSMSKSPNGQKRSRSRSPHDQGFCVYLKGLPFESENKHVIDFFKKLDIVEDSIYIAYGPNGKALGEGFVEFRNETDYKAALCHHKQYIGNRFIQVHPITKKAMLEKIDMIRKRLQNFNYDQREIMINAEGETSPSKLCAHISNIPYNITKMEILQFLEGLAIEESSIQILVDTNGQGLGQALVQFKTEEDARKSERLHRKKLNGRDVVLRVISLEEMREIERNPPSQGKKLLKMPLQGGAAMPGVQNAGGDEHSFLSGSSKDAKSGPPFHFPGNFSGSNVFGPPLPPPGIGGGGFGDSRPGMPAIGNSGLPVLPGAGIDVPGFGGGPSNLTGPSGFGGGPQNFGNGPGNLTGPPNFGSGPPGLNTGLGHMSGPPGFGPGPGPGNMHMTGPPGFGAGSGKPGPTVIKVQNMPFTVSVDEILDFFYGYQVIPGSVCLKYSEKGMPTGEAMVAFESRDEAMAAVVDLNDRPIGSRKVKLMAQCVIKVELSISCDNLIDKDVGSKSDPLCVLLQNVGGDQWTELDRTEKIKNCQNPQFSKKLLIDYYFEKVQKLKFGIYDIDNKSYDLSDDDYLGGAECTLGQIVSSKILVQPLMLKKGKPAGKGIVTICAEEIKDTRVVSIDVEARNLDKKDFLGKSDPFLEFYKQSDSGKWQLVYRSEVIKNNLNPCWKKFSVPLQTFCSGDFNRQIKVHCYDYDSDGSHDLIGSFESTLSQIQKAGDSSPVEFECIHPEKKRKKKSYKNSGIIRLKSCKIETEYSFLDYIMGGCQINFTVGIDFTGSNGDPKSPDSLHYISPNGINEYLTAIWSVGGVVQDYDTDKLFPAFGFGAQVPPDWQVSHEFALNFNPANPYCQGIQGIIDAYRQALPQIRLYGPTNFSPIINHVARFAAHAMQEKTASQYYVLLIITDGEITDLDQTRQAIVNASKLPMSIIIIGVGSAEFKAMEVLDGDDGVLKSLAGEPVARDIVQFVPFRQFQNAPREALSQAVLAEIPKQLVSYFKKAGLTPVKLPSEKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDV7</accession>
    <name>A0A6I9YDV7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">4F2 cell-surface antigen heavy chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106549173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922224.1">
        <property type="nucleotide sequence ID" value="XM_014066749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDV7"/>
    <dbReference type="GeneID" id="106549173"/>
    <dbReference type="KEGG" id="tsr:106549173"/>
    <dbReference type="OrthoDB" id="204980at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015173">
        <property type="term" value="F:aromatic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015180">
        <property type="term" value="F:L-alanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015190">
        <property type="term" value="F:L-leucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904273">
        <property type="term" value="P:L-alanine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903801">
        <property type="term" value="P:L-leucine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015823">
        <property type="term" value="P:phenylalanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
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        <property type="entry name" value="SLC3A2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46673">
        <property type="entry name" value="4F2 CELL-SURFACE ANTIGEN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46673:SF2">
        <property type="entry name" value="4F2 CELL-SURFACE ANTIGEN HEAVY CHAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="2">
        <location>
            <begin position="92"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013922224.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="27484" checksum="CCBE2B69723B16F2" modified="2020-10-07" version="1">MGKLGDGATPMHSEHLPLLAKAAPSATYLSQQEVAEQGSAAPWPGLRRALISLLAGLFACMLLLAVALLLSLPRPRPALGWWQKAAFYHLPPSSFLDSDGDGWGDLAGVRQQLDQLATLPIQALVLGSILEGGGANLTQIVPARGSLAQLQALLTDGHKRGIRILLELPPWGEEGDASAESNQTVKLLKEALQFWQSEGVHGFLVAKDPAWCLLTVSFTTGNQPVIYNNNLSGMGMEQHPVITLNHAAASFAWHNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEV4</accession>
    <name>A0A6I9YEV4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synaptogyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SYNGR3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synaptogyrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922713.1">
        <property type="nucleotide sequence ID" value="XM_014067238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEV4"/>
    <dbReference type="GeneID" id="106549547"/>
    <dbReference type="KEGG" id="tsr:106549547"/>
    <dbReference type="CTD" id="9143"/>
    <dbReference type="OrthoDB" id="10041611at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016579">
        <property type="entry name" value="Synaptogyrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838:SF8">
        <property type="entry name" value="SYNAPTOGYRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011282">
        <property type="entry name" value="Synaptogyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="20"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="193"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR011282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922713.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="24900" checksum="271CC0F7F1F82566" modified="2020-10-07" version="1">MEGASFGAGRAGAAVDPAEFAKRPQTLLRVLAGVFSIVVFGSIINEGYVNTDTSPVLHCIFNSNEGACNYGIAIGLLAFLACSFFLVMDLYFQQISSVKDRKRIVLLDLGFSGFWSFLWFVAFCFLANQWQRTPASKGASQGGDAARAAIAFSFFSIISWVALTIKALQRYRMGTDMSLFATDQFGPDPMAGYPGHPPTSGGIESTETYQSPPFSETLNTSPKGYQVPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEW7</accession>
    <name>A0A6I9YEW7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Autism susceptibility gene 2 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106549545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922711.1">
        <property type="nucleotide sequence ID" value="XM_014067236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEW7"/>
    <dbReference type="GeneID" id="106549545"/>
    <dbReference type="KEGG" id="tsr:106549545"/>
    <dbReference type="OrthoDB" id="10060000at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023246">
        <property type="entry name" value="AUTS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429">
        <property type="entry name" value="FIBROSIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429:SF20">
        <property type="entry name" value="FIBROSIN-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15336">
        <property type="entry name" value="Auts2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02044">
        <property type="entry name" value="FIBROSIN1LPF"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013922711.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="22222" checksum="6CB5F9BA3D832976" modified="2020-10-07" version="1">MLPHPGPFGSLQGAFQPKTSNPIDVASRPSTVHHTLLQKTPGVADAYRAPVRKPGKWCAVHVQIAWQIYHHQQKIKQMQLDPHKLEIGGKLDLFSRPPAPGVFPGFHYSQDLARPLFSSTGSAHPNSTPFGSSPHHSSFLPTSHLADPFTRSSTFSGLGNLSSNAFGGLGSHTLSHSSLFAHKDGPNIQTFGSPHEPWNRLHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF69</accession>
    <name>A0A6I9YF69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">DDB1- and CUL4-associated factor 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Serine/threonine-protein kinase VPRBP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">LOC106549629</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="25">Component of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex. Interacts with DDB1; the interaction is direct. Also forms a ternary complex with DDA1 and DDB1. Interacts with NF2 (via FERM domain). Component of the EDVP complex, a E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1. Interacts with DYRK2; the interaction is direct. Interacts with RAG1; the interaction is direct. Interacts with LLGL1 and LLGL2. Interacts with histone H3. Interacts with ESR1 and LATS1; probably recruited by LATS1 to promote ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation. Directly interacts with TET1, TET2 and TET3 (via C-terminus). Interacts with CEP78; promoting DCAF1 localization to centrosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the VPRBP/DCAF1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013922807.1">
        <property type="nucleotide sequence ID" value="XM_014067332.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549629"/>
    <dbReference type="KEGG" id="tsr:106549629"/>
    <dbReference type="OrthoDB" id="27563at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990244">
        <property type="term" value="F:histone H2AT120 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030331">
        <property type="term" value="F:nuclear estrogen receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000055">
        <property type="entry name" value="DDB1 and CUL4-associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033270">
        <property type="entry name" value="VPRBP/DCAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13129:SF4">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13129">
        <property type="entry name" value="VPRBP PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="20" id="KW-0804">Transcription</keyword>
    <keyword evidence="19" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="22"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="247"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="790"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1206"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="28">
        <location>
            <begin position="1208"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="28">
        <location>
            <begin position="1303"/>
            <end position="1314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00063313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00071147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00078221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013922807.1"/>
        </source>
    </evidence>
    <sequence length="1320" mass="145592" checksum="1D25C47EA012F2EB" modified="2020-10-07" version="1">MGTSGILRKALSSKPNLPWAVVSPMMKSDDKENGNQEVELANEGPANVVRSMEEWHSTRQENAPPPGRTEQQQKKESSGLNSAGYLGRAKSVPRSLSARGSAGSEEDCGTEGAPDPQLQRDLIIERGGRESEQTPNQLPESFQAVLAKANADGIASGMQHQFQGARHRSTRVAPREHNVQPEALASRDTRASSASTASEGSESEGEEQKDLGFSEDEDASVPESPTITGLFLPALFSSLLRKAKATADLDPPPTENQSSATPSTSGGKRKQGPFHRPQVPPMDATVAALASASTMVTGDISNCLKAEDRRAELVMRKTFQAISWAIKVAAAASFFKRSTLLWLEQIRDRTPASDMRLQQDIIKLMAATQFSIACSYTHEQIVEMMEFLIEYGPGQLYWDPAEVFLKLCCVQLMLQLISIACNWKTYYARNDTVRYALDVLAILTVVPKIQLQLAEAVDTVDEGGTSISIVGISIILGLAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRALPQTPRNSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSEHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATILTKEADLPMTAASHSSAFTPVPATSSPVSLPRTPRIANGIAARLTNHPSVASGASSVHPQPRPAGCQGPLALPGPSHATNAPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPTNFTSRLTRRAAFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGHLKLYNVFSGQEEASYKCHNSAITHLEPSRDGSLLLTSASWSQPLSALWGMKSVFETKHSFTDDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKKNSATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMTISGVFHPNGLEVIVNTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMRSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDAMNMDTVCRLYEVGRQRLAEDEEDEDDDQEEEEQEEDDDEDEDDTDDLDELDTDQLLEADLEEEENNENAGEDGENDFSPSDDEEVANLLEEGDEGEDDDSDADEEVELILGDTDSSDNLDLEDDIILSLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFU8</accession>
    <name>A0A6I9YFU8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106549905</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923138.1">
        <property type="nucleotide sequence ID" value="XM_014067663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFU8"/>
    <dbReference type="GeneID" id="106549905"/>
    <dbReference type="KEGG" id="tsr:106549905"/>
    <dbReference type="OrthoDB" id="9049773at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028750">
        <property type="entry name" value="CEP350/CC187"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13958:SF3">
        <property type="entry name" value="CAP-GLY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13958">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN 350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923138.1"/>
        </source>
    </evidence>
    <sequence length="709" mass="76970" checksum="C3B9AB2257FD7601" modified="2020-10-07" version="1">MATVNKESWEAFHECFECFIQANDLVEVSFDRKRGFFLTDPSCISKDTSVRHNSPNGSQGTGGPSPTAEKQISILTRLKQEQAEIRHLQNIGRAAHRERKLLLQQQKELLEVQRTTTQLWLQLAGELPHPIPENYNLQGVGLLEKETFEPSSKAGPLLATPSEGTERRSSREAGKKHLAESKDILPPENRAAEGFEKWKEGKETTIQDLYDLSGRRSRDPTLGDGRTADHLSSRGGKEYDHGLTKNSQDDHPGSPLDKDPEDSSSAGFPSALRGFSPYPEKSPSGLEFHKGYAVLVHLSGSSPSDLEDEGLQDTDVSLPEEFLFQEPLPEQLGVSTHHQAPAVPRIVINTEDSPSSSEKKPQGELSGGDLESRDSACALYSQEKIWEDTRGRERLEESDSVILSNHGRVQESPSPVAKKDRVHTPSTAASLNISEELSCTENGGDEMTSGSPVDPGMVFFSTNVPFQSPGAEGKKDPAPSSNEAGEDVEDENPPRFGNQEAIVTLAPVFSLNIGAPEDPEDPLHGPKHSRGHRFPISSGHLPASGDQPGCANRSTDECVSHKPVETSVQLHKTSMGNGVLVEEHRKSSPARNQSINKAEMPTVRLSSLQEEDLMENEGDAMLLPELDDDVVSPVDEMLTYGSSDLPSSSEKDVSSWSTDLPAPPENHGEANSSLLNFPSPPDPVGSSEAEELSSPRDLPAEGVGSSLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFZ3</accession>
    <name>A0A6I9YFZ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Leucine-rich repeat and WD repeat-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LRWD1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923097.1">
        <property type="nucleotide sequence ID" value="XM_014067622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFZ3"/>
    <dbReference type="GeneID" id="106549865"/>
    <dbReference type="KEGG" id="tsr:106549865"/>
    <dbReference type="CTD" id="222229"/>
    <dbReference type="OrthoDB" id="7318948at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000488">
        <property type="entry name" value="Leucine-rich repeat and WD repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052489">
        <property type="entry name" value="LRWD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056160">
        <property type="entry name" value="WD_LRWD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24370:SF10">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24370">
        <property type="entry name" value="OPTICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23215">
        <property type="entry name" value="WD_LRWD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Leucine-rich repeat and WD repeat-containing protein 1 WD" evidence="9">
        <location>
            <begin position="120"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013923097.1"/>
        </source>
    </evidence>
    <sequence length="515" mass="57528" checksum="B6F5024CDE186638" modified="2020-10-07" version="1">MLHQSFGPVTPSKRKGARSKGRAKSKRSHSQSSSKEEASPSPKKLSRLQEEHSTAATRASRPQTKESQTPPDPDGAHRNGGRHKATKAQSHKKATQQKEEHKEEEDERDACSTPEKDLEGEETITLEPVHFLQCHSKANNRDDFKTQLWACVFEPPLDCGTRKDPVVSSSRTAATCGGDSVCLVDCETGIVLKKYKVTGEEFYTVTWTTLTMVTSDGRKKKHNVLAAAGRRGIVKLIHVTADYCYGEIKAHKKPIATACFSPTCETHLFTASYDKKICAWDIGIPDCDYNFQERQLMVLDTISTPLRIALVPSCPDRFLVAGCEQGCFAWDIRLDDKPQKNRAFEVEFQFPDDEGNDTSSHRVDGLAFLNEDIVVTKSSKRESINLWSWSRSFKQGKSGRRTVQAVILAELEWSSTDLPYLTLSTCPTEEYVFCGDEKGSIWMYDLSNHLSALCATKEDCPTERIGPSQILKWPELKANGELLTDVLVNNVVTDPTFTYLVALTDVNIAAIWKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGB2</accession>
    <name>A0A6I9YGB2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Zinc finger protein 208</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">LOC106550056</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923312.1">
        <property type="nucleotide sequence ID" value="XM_014067837.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923313.1">
        <property type="nucleotide sequence ID" value="XM_014067838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGB2"/>
    <dbReference type="GeneID" id="106550056"/>
    <dbReference type="KEGG" id="tsr:106550056"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000113">
        <property type="entry name" value="zinc finger protein 699 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF377">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="710"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="749"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="786"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="824"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="862"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="901"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="938"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="976"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013923313.1"/>
        </source>
    </evidence>
    <sequence length="1020" mass="117197" checksum="8F17052B5993DF3A" modified="2020-10-07" version="1">MAALESAFSEEAMADINPALDRENLAATDNEGLATDQLTTTEKVALKNSVNAEAVWEYPWQSDKVVTNLSVPHQDGNISVKDEEANSQSSKQINNHDNQNKNLYFCDVRLKKDFQQPSLFAQHKNVFCPSKVLMQNKQLNWDQLEIGSPYQPDQNRQTKNCSIGDQCGENNQSLSYLPQYCKNILLKENSAWQHQNSQSEASSLISEACNQSTLVPVPLRAENIIEYIDEAVGPNPIQVTDQRTETKHLHICDQCGKEYEWLSELIQHQLFFGGEADRQCQLHMEGRNPSHLAPVPILFQGNNMCATSWETFQQKSRYHNTHAKIGKDFCRFSLPKRHTLPREELTRQNESKINKSSTLSFVPLVRRNTFADSCEMSNVNSMLTRSYTGKQHSDQCGRCYHCLCAQQHLDNFELKSDTDELFNLGAYCPKSLVPIPSLAEKIFSENSKIPIEQKNYAKNLNTFNCDKNDFQRHLLTKHHLHVPLGGEADILDQLKIQAGSLSQFQIERKVIESSESVKPQSNLTTNQGFQIKTECEKDFQWSSALTQHQVVNSGKVNWKQCPSKVDDQNDSSPDLMDLDQPAHLKTNQLTEKHKPYACGHCGKSFKWPSGLVRHEQTHYRTKLIKGKCTKLNKSYICGHCGKTFQWPELVQHKYMHSRKKLLRAKDTKPNKYYTRSQCGRSFQSLSQLAQHEHTHSRSKLVSRKLKPKPHICNQCGKGFTWPSDLARHQQTHSKKIILKGKGIKLKKYYTCSQCTKSFQRPSQLAQHEHTHSRSKLVSSKLKLKSHICNHCGKGFTWPSDLARHQQTHSKTIILKGKGIKPKKYYTRQCTKSFQRPFQFTQHEHTHSRSKLVSSKLKLNKSCICDQCGKSFKWPSDLARHQQTHSKKIILKGKDIKLKKYHTCSQCTKSFQRPSQLAQHEHTHSRSKLVSRKLKPKPYICNQCGKGFMWPSDLSRHQQTHSKTILKGKGIKLKKYYTCSQCTKSFQRPFQLAQHEHTHSRSKLVSRKLKLNKSCICEQTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGP1</accession>
    <name>A0A6I9YGP1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">General transcription factor II-I-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106550167</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923463.1">
        <property type="nucleotide sequence ID" value="XM_014067988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGP1"/>
    <dbReference type="GeneID" id="106550167"/>
    <dbReference type="KEGG" id="tsr:106550167"/>
    <dbReference type="OrthoDB" id="10072451at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1460.10:FF:000004">
        <property type="entry name" value="general transcription factor II-I isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1460.10">
        <property type="entry name" value="GTF2I-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004212">
        <property type="entry name" value="GTF2I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036647">
        <property type="entry name" value="GTF2I-like_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46304:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46304">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02946">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117773">
        <property type="entry name" value="GTF2I-like repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51139">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="272"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013923463.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="48248" checksum="ABCFD8B10214F22D" modified="2020-10-07" version="1">MQRIAKMAQAAMAAAAASSHDEDSSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCINEEAKAVELQKAKSSSLDNRMTVDVVEMEALKKSVEDYFCICYGKALGKSTVVPVPYDKIQRDPSALIVHGLPEGLAFKHPSSYDVSTLKWILENKSGISFIIKRPFLEPKKHLGRLSDSSHMMTSPGISSIQVKTEPNEDSGLEMVTVKEESEDPEYYQFNIPAGPSETSAIDEKIAMAKSYTGSNQGSDCSEGTDVELPVEDSSQHATSETSEDPEVEVTIEDDDDYLPPHKKTKSIEPVNDASNVGRRKAREFNFEKWNARITDLRKQVEELFERKYAEAINAKGPVSIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHF4</accession>
    <name>A0A6I9YHF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tectonin beta-propeller repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TECPR1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TECPR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923300.1">
        <property type="nucleotide sequence ID" value="XM_014067825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHF4"/>
    <dbReference type="GeneID" id="106550045"/>
    <dbReference type="KEGG" id="tsr:106550045"/>
    <dbReference type="CTD" id="25851"/>
    <dbReference type="OrthoDB" id="72441at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000690">
        <property type="entry name" value="Tectonin beta-propeller repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923300.1"/>
        </source>
    </evidence>
    <sequence length="166" mass="19854" checksum="5BB776A0EC2B63C8" modified="2020-10-07" version="1">MEALTPCSQSLALSISPSHTAQWRKQIFRQLSERTHRELDNFRHYEQAVEQSVWVKTGTLQWWRSWKPYRWIDVQVALEQFTANDGTRDSILFIYYTHNEEKKYVHMFLNEVTILVEILNEAKHSFALYTPERTKQRWPVRLATSTEQEMHDWVCLERCSLSLCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI34</accession>
    <name>A0A6I9YI34_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc transporter ZIP11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Solute carrier family 39 member 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Zrt- and Irt-like protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">SLC39A11</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923857.1">
        <property type="nucleotide sequence ID" value="XM_014068382.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923858.1">
        <property type="nucleotide sequence ID" value="XM_014068383.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923859.1">
        <property type="nucleotide sequence ID" value="XM_014068384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI34"/>
    <dbReference type="GeneID" id="106550462"/>
    <dbReference type="KEGG" id="tsr:106550462"/>
    <dbReference type="CTD" id="201266"/>
    <dbReference type="OrthoDB" id="262547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF211">
        <property type="entry name" value="ZINC TRANSPORTER ZIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="146"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013923858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013923859.1"/>
        </source>
    </evidence>
    <sequence length="342" mass="36562" checksum="B1236F4ECBDAAAF4" modified="2020-10-07" version="1">MILGYNPVLQALLGTLLTWGLTAAGSAFVFIFSSRQRRILDGSLGFAAGVMLAASYWSLLEPAIELAEESGKFGALAFLPVAVGFTLGAAFVYFTDLLIPWLDINVSPQTSLALNYDSRVIKEKYEHQATDRQEYENELSIRIGRTALHSDKGENGEPYQRRKGAGHSQPDLPAVSHTLKDNVPVPASSSWRRIMLLIFAITIHNIPEGLAVGVGFGAVGKSPSATFESARNLAIGIGIQNFPEGLAVSLPLHGAGFSTWRAFWYGQLSGMVEPLAGIFGAFAIVLAEPLLPYALAFAAGAMVYVVMDDIIPEAQISGNGKLASWTAILGFVVMMSLDVGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI56</accession>
    <name>A0A6I9YI56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pecanex-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pecanex family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923891.1">
        <property type="nucleotide sequence ID" value="XM_014068416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI56"/>
    <dbReference type="GeneID" id="106550490"/>
    <dbReference type="KEGG" id="tsr:106550490"/>
    <dbReference type="OrthoDB" id="10037631at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039797">
        <property type="entry name" value="Pecanex"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12372">
        <property type="entry name" value="PECANEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12372:SF4">
        <property type="entry name" value="PECANEX-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923891.1"/>
        </source>
    </evidence>
    <sequence length="116" mass="13172" checksum="A920F71443AA1324" modified="2020-10-07" version="1">MGSQVLQILRQGVWASLTGGWFFDPHQSTFSNCFHLYTWIFLLVLPFLLYMVMPPSLMVAGIYCTAVAVFFTTVKTVNYRLHTMFDQGEIVEKNSVLVESGKLDERAAADDDNLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIP3</accession>
    <name>A0A6I9YIP3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Integral membrane protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106550665</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of amyloid-beta peptide production. May inhibit the processing of APP by blocking its access to alpha- and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with BACE1. Interacts with APP. Interacts with STMN2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Lysosome membrane</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ITM2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924087.1">
        <property type="nucleotide sequence ID" value="XM_014068612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIP3"/>
    <dbReference type="GeneID" id="106550665"/>
    <dbReference type="KEGG" id="tsr:106550665"/>
    <dbReference type="OrthoDB" id="9982095at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042985">
        <property type="term" value="P:negative regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040145">
        <property type="entry name" value="ITM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962:SF5">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962">
        <property type="entry name" value="INTEGRAL TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="17">
        <location>
            <begin position="143"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013924087.1"/>
        </source>
    </evidence>
    <sequence length="274" mass="31474" checksum="4CBB5E7109555F57" modified="2020-10-07" version="1">MVKIGFQPVTAELKPEKEAAVATRHGPPAKGLVLSQDAEKQPLSLPQQSWRSLTCLFFLALGFLLLLLGLILTSTYIYKYLSLTHSVAHMGMDMQRTEYYNYEEGGLECDYFEFDDSSETRTVYEDVKIFLGEEYEEISIPRPNVGHPADIIHDFQQGFTAYYDWVVNKCYIGELNTTIVMPLKHFWELTLNIKKGVYLSQSNMIQEERMVSQHATDPDLFGSYIHKLCQGKETFMLGHRIARGHIHQRATEKCHHIRHFANTFVVDTAICQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKH3</accession>
    <name>A0A6I9YKH3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Adenylate kinase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106551054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924539.1">
        <property type="nucleotide sequence ID" value="XM_014069064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKH3"/>
    <dbReference type="GeneID" id="106551054"/>
    <dbReference type="KEGG" id="tsr:106551054"/>
    <dbReference type="OrthoDB" id="6436361at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924539.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="27713" checksum="FE5D699802E3C4B7" modified="2020-10-07" version="1">MDLFAPQLCLVEAPRTFHRPRLELLELVHSEPAAELQQNQISFPVTSGLSWPWKKPPGLPSPHPLQNLLDGGVLCRVTGNVTGRDRPSLKAVNEEDWRLRDKALQLSDQMLNQTTLHSSIKDIVTELLKEAILANMENSKGFLIDGYPQEIQHGELFENEIGEPSLVLCMDCSSKTMSSRLLQKSQCLEDNIETIMKCIEAYYKTAEPLIAYYENRIPVFKINAEGTPEEVFLEICTSVNTFLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YL62</accession>
    <name>A0A6I9YL62_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger protein with KRAB and SCAN domains 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106551312</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924876.1">
        <property type="nucleotide sequence ID" value="XM_014069401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YL62"/>
    <dbReference type="GeneID" id="106551312"/>
    <dbReference type="KEGG" id="tsr:106551312"/>
    <dbReference type="OrthoDB" id="9042071at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SCAN box" evidence="4">
        <location>
            <begin position="41"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="5">
        <location>
            <begin position="201"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013924876.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="28851" checksum="A479795A7ADC45E8" modified="2020-10-07" version="1">MEQNLAGSEAGRSSDALQVELGAAKMMPGESIHIGDARCLHFRHFRYPEIWGPREVFNQLRDLCFLWLQPEHRTKNQILDLVILEQFLAVLPPEMESWLRECGPETSSQAVALAEGFLLSKAEEKEEQQGQENFPDLANEMEKTPSSIRRRTFFRWIVEEEEPEQVASCQGEGMTPVIQSWWPPYGNGIETAHEEPPQDLVTFEDVAVYFTDEEWALLDPDQRALHVEVMEENCGNLAFLAGDSWKAKTEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLA7</accession>
    <name>A0A6I9YLA7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase RFWD2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106551351</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924921.1">
        <property type="nucleotide sequence ID" value="XM_014069446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLA7"/>
    <dbReference type="GeneID" id="106551351"/>
    <dbReference type="KEGG" id="tsr:106551351"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16504">
        <property type="entry name" value="RING-HC_COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042755">
        <property type="entry name" value="COP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44080">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44080:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="160"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="90"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924921.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="43345" checksum="B03FCD548BAD0860" modified="2020-10-07" version="1">MIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPSGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMTRLSDDSRTSNQLDEFQECLFKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQGCSIYQSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLF5</accession>
    <name>A0A6I9YLF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LOW QUALITY PROTEIN: HLA class II histocompatibility antigen, DRB1-10 beta chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106551112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924600.1">
        <property type="nucleotide sequence ID" value="XM_014069125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLF5"/>
    <dbReference type="GeneID" id="106551112"/>
    <dbReference type="KEGG" id="tsr:106551112"/>
    <dbReference type="OrthoDB" id="9038477at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF99">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DRB1 BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924600.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="14877" checksum="7A2E18F12C75722A" modified="2020-10-07" version="1">MHPASLNTILLSTVMGFYRMEIEVRWLKNGQLEKEGVAFGGLLQNGDWTYQLQVMLETQPQRGGDDYTCKVEHTTLEAPITVQWELRSSSSARSKLWTGIMGVVIGVAFLAVGLFSYLKSKKATPTQPPAALMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YM76</accession>
    <name>A0A6I9YM76_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">28S ribosomal protein S35, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MRPS35</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924910.1">
        <property type="nucleotide sequence ID" value="XM_014069435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YM76"/>
    <dbReference type="GeneID" id="106551341"/>
    <dbReference type="KEGG" id="tsr:106551341"/>
    <dbReference type="CTD" id="60488"/>
    <dbReference type="OrthoDB" id="283424at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019349">
        <property type="entry name" value="Ribosomal_mS35_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039848">
        <property type="entry name" value="Ribosomal_mS35_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10213">
        <property type="entry name" value="MRP-S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS35 mitochondrial conserved" evidence="1">
        <location>
            <begin position="166"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924910.1"/>
        </source>
    </evidence>
    <sequence length="326" mass="37059" checksum="6179FE5018196F69" modified="2020-10-07" version="1">MALAWRRCLVLWRRRRPSSAGYASLGGAAQPQRKGTLKETLAERRAQKELIYGNTQRSDKMEPDQDWTSVYPTAASFKPSAVPLPIRMGYPVKGGAPLAKEGNLELLKIPNFLHLTPVAIKKHCAALKDFCTEWPSALDSEEKCLQHFPLEIGTSDYVSAGPSLRNPKARVVTLTVKLSGLNLDDHAKKKFIKLVGERYCGKTDSVTITTDRCPLRKQNYDYAMYLLTVLFHESWKTEPWEKEITEADMLEYVWENSVSEKNALKTLLQIRAAEKAEEKSREELLASEVMQRYKKSVVLLKNEGETEENLLEYKNSVKRLLNIQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPT5</accession>
    <name>A0A6I9YPT5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 13D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">VPS13D</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925695.1">
        <property type="nucleotide sequence ID" value="XM_014070220.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106552033"/>
    <dbReference type="KEGG" id="tsr:106552033"/>
    <dbReference type="CTD" id="55187"/>
    <dbReference type="OrthoDB" id="272810at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14306">
        <property type="entry name" value="UBA_VP13D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000107">
        <property type="entry name" value="Vacuolar protein sorting 13 homolog D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041969">
        <property type="entry name" value="VP13D_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056747">
        <property type="entry name" value="VPS13-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF141">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25033">
        <property type="entry name" value="VPS13_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="2523"/>
            <end position="2563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="766"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1443"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1744"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2078"/>
            <end position="2146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2485"/>
            <end position="2512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="771"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2129"/>
            <end position="2138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="2830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925695.1"/>
        </source>
    </evidence>
    <sequence length="2830" mass="316648" checksum="2C7167BF75C4E6C0" modified="2020-10-07" version="1">MLEGLIAWVLNTYLGKYVSNLNTDQLSVALLKGAVELENLPLKKDALKEVLPFEVKAGFIGKITLQIPFYRPHIDPWVISVSKLYLIGSPEKEEEFVEARERLLDKENKKALLLALEEKWKKEREQIGESYWYSVTASVVTKIVENIELNIQDVHLRFEDGVSNPSQPFAFGICIKNVSMQNATDEPVEELMKKKRLDISDFSIYWDSQCSLWGDMLPGELQEAMDNSMERRDHDYIVEPVCTSALLKRNCSKEPLRSRNMPRLEFDIQLETIPLKLSQMQYRQIMDFLKELDRKERQLKFRKWRPKVTVTGNCQAWWLFALNAHLAEIREQKERYSWEFALRRARDAVLYTELYCNKLKELSLTEEEQEEVERIEDEQTFEELKILREIVYNQLQELAESIKEPLSDSLDSASEVALEGGRNSSSGMLRYLQSWFPGWGGWHSQSQEELETETIGGLLSDPAEEWRPEEVAGADDFFDPATDVSSINTFTKRDHVLVKLNLWLQGGSVTLLHAEKRSGQASERAFMQLQFSGVTILAETLPRRNSSLLKVQLGSLFLRDLATEGTIFPVLVFPNPLKEVGCASSVSLQTSSSDTSNQSSEDPVPVFEMLYEKNPARSNFERRLEVSTQPLNIIYNPQAIKKVADFFYKGRVHSSGFGCQSELELRVAEAARRQYNRLKMQTKAEIRQTIDRLLVGDFIEESKRWSICLDISAPQVIFPDDFKFSDPVLVVIDLGRMLLTNSQEEHRKKSPDGPSLELSDLSDEEYKTPLATPPNSPPPELNPTGEDKSSEEFPGVEISEEQLQAHLMSKKMYETYTLSFNDLQIMVGRVKDNWKYAQDIDVGPTHVVEKFNVHLQLERRLIYTSDPKYPGAVLSGNLPDLKIHINEDKIVALNNCFGRLSTPDPEGSQAGKEAPLSSLEQRGSLHNSVVNLTQSVILVEQHTKEVLVESQLLLAEFRVNCMQLAVESNGRYISVLKVFGTNAHFVKRPYDVEVSLTVHGLLLVDTMQTYGADFDLLMASHKNLSFDLPTGSLRDSKAQSPVNGLSETLLVGDTSLAEKCTQASSKRLSGTSSKRDESLIKLEYQFVTAECPSMNLDSSLQVLTVQMNNLDIILNPETIVELIGFLQKSFPKEKDEGSPQPLLTDAENTFGEQENFQSTHSQSIEVAVEIHWLNVLLLRTVTLVNREKFGRKIATASIGGTRVNVSMGSRLEVNGSLGCLHLMDLTQDNVKNQYVVSIGNTERYESLIRDVGYARPTFPQFEDASGLPDALCFTFLEQSAAECSLLLQMASLHYNHSAKFLKEIALSLDELEENFRGMLKSAASKVTTVLATKTAEYSEMVSLFDTTPRSRDPFNLEHDDGEEQAPLYPKTETIKLVLNVKIESPVVSIPRKPGSPELLVGHLGQISIQNFVTGEDNLRSDSLQVEIRDIKMYSLNCSHLAGKKDSPLEAPPELPSPSGYQEESQFTRHDFFESLHKGQAFHILNNATIQFILEKIPIEPNAELAFSPSDSVNISSLMRIEGKFVNPLQVILAKHVYEQVMQTMDNLVCNEDLTKPPSSFPSSTYSSLASPSACLRSLSADFPAGRKENGFFSHATHSDAKGPRPVQGASQSFTQIQAMFHIAWLQVQLSGDLTLGAQGLVSLTFQDFDVEFSKDHPHTLSIQTTLRSLLMEDLLVKNPDSPYRNLMVSHGAPKPSSLAHKEYLSQSCPLVSNVEYPDMPRSLPSHMEEAPNVFQFYQRPSCVSHPRHHEGGEEGCPLTPPPSPTARELAAPSGRSDFDDSLVHINVLLVDKKHPEFSSRYDRIHRSVDIDFNCLDILITLQTWVVILDFFSIGSTAENHGVRLTPSGEVQQAAKSEFNPSPDDEAQEESVNSKLDLKVHSLSMVLNKATNELAKANVSKLVTHMETREGDLTFRGSIGSLSLSDLTSHGELYRERFTTSGEEALIFHIHKYGRPDLFLQRECDVRVSLRMASVQYIHTQRFQAEVISFIQHFTQLQDVLGRQRAAVEGQTVRDQAQRAARILLDIEAGAPVLLIPESSRSSQLIVANLGKLKIKNRFLVAGTSATFSLKDKDPVLFSSPLGTPKHNVKKTPSGDESKKAPHFPEGLPITELSSGPILGGQKSEHAPETPSSPLSSAENPGKRRSCYSQEGLLPPLSLPLLDEFWTVLSGEVYICLSFLINMVNVSLELTGPQPKQGSLARFDFKKSKLLFESCSNQTRTINLVSHSMMAFDTRYGGQAASAETPNVFNCIFQPSKSNHSQGAIQIELHYRSTKDSSSFTVVLNNLRVFLIFDWLVLVHDFLQTPSDPRKPAHPSPASQRSLGGETAVVPKTIKCGIVTKRSSLQISTEKQLEVKVNVTDTEFVVVEDTTCSDTNAVILKGTTVLTYKPKVVDHPFSGSLSGIEVFSCRLGNEQETALSILDPALIQVELVGNSSYPNGSGLLDAFNGGDFPPLLEIQLQTLDIRLSYNDVQLFLAIAKSISKQTSGVLPSPPATDSPAPSGPSEISGTSAERMEGAKRLLDPVLEVQLARLQELGFSLDDCRKALLDCQGQLKKAASWLFKNAEPLKRSGFESCGTDGQSSRALRLVSGVEVKAESICICFIDDCMDCDVPLAELTFSRLNFLQHLRASPEGYAHFTLSGDYYNRELSGWEPFIEPWPCSVFWQRQPASRLHPSRLKLEVKAKHRLDVNITSRLIEQYASTKQSWMADYCSQEKEVTDVESEDWVGSSVDPPSFRQGLPLAYLRTRSTASLTNLEHQMYARAEMKAPKRRQPFVPFALRNHTGCTLWFATMTTTPTRAALLHSGSPPVLPEEKETFLDEAHKVSEWREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPW5</accession>
    <name>A0A6I9YPW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">FYVE, RhoGEF and PH domain-containing protein 5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FGD5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925954.1">
        <property type="nucleotide sequence ID" value="XM_014070479.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106552255"/>
    <dbReference type="KEGG" id="tsr:106552255"/>
    <dbReference type="CTD" id="152273"/>
    <dbReference type="OrthoDB" id="245697at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15742">
        <property type="entry name" value="FYVE_FGD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15792">
        <property type="entry name" value="PH1_FGD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13237">
        <property type="entry name" value="PH2_FGD5_FGD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF13">
        <property type="entry name" value="FYVE, RHOGEF AND PH DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="11">
        <location>
            <begin position="1028"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="1246"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="1375"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="1484"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="143"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="328"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="412"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="492"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="809"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="882"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="998"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="420"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="811"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="835"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925954.1"/>
        </source>
    </evidence>
    <sequence length="1581" mass="177096" checksum="8BF8C475E1BFC3A1" modified="2020-10-07" version="1">MSHADLTKPPLAPKPKIFSHLASIAASKFPQSLSRAGTLHNSNSMSRGPKPPIAPKPKISADNDPAILQRSNVYANNTFNKYPNGKLEAELYEENQCNNTEGPEPEDSNEYIVIPDVELTDKTYSDFEQEPAVNEILHTLGPVGEEDGNCKDSNDVGDGPINVMKNKDTESVGDNSVESTEIPALDSPEFESEKEDGTRDEYEKVDSENPPSEIQMNCADETLSKLDLINDNNEYNMDRKILNGESLKETIYDDIILRHLEEPISAGEGLPLICKGLNAIQFLEEEQLNTDLELGPANHNDNNSAWSRERKLTEDADVNTDAQIANDSEVDTELEPSGSEPGIETFSGATDEMDEKEDEIDLNPQGEDLDNVCQTTEVKNVVEIPETECETGEGFGEQNETIDTETIDDSCQIIPFGNEGSEDAQDSPDEDLGEDLSEKETEMPDNNMSLSSEDTNGHKSDSQLKSDVLLGDHSASECTLEDTHLAHQILEEDHNNKEETAPNPTRETSTENSGLSTGELAKLEINSANECVIEEGTVVSKVLFGPDSNGEVASDSLDDPYVLGDTLPTANEILYAGRETQEADIQNSSSDFREGENTENENHLLSIDRKNIVTRTRSHSGKVPGYVPETVPEETGTDAELQTLSTNTMITENVRHSLCWAEGKPIELARTLPAKPRRFILYPRSYSVEGRDIPVALYRETDNSILDYNRLNRTDELALPYVIGSSGSFSQRNHLTSSGVSTPSSVVDIPPPFELACITKKPITKSSPSLLIDSESPDKYCKKKKSSFKKFLTLKFKKKAENKVHVDVHVSSSRSSSESSYHGPSRVMELDRRSLSSSPQLLSRTAKLRASESPTVLFYKDGKRKGTPKMFSRSVSRVESFEDRSRPPFMPLPLTKPRSISFPNADTSDYENIPAMNSDYENIQIPPRRPTRAGTFTEFFEDPSRALSAANENDGYVDMSSFAAFENNKQQATNQETESAYTEPYKVCPISVIPLEDVTSDEEQKSSEDEESNQGDSNIIHKKEGQSRAQLVAQELVSSEKVYVEMLRLLHTDFHEAVLKVLGDECDNDHEETKLKEGLKEIPEIYNLHQEILGELEEKVLNWEEHQKVADIFLSRESGFNHHTTYIMQFDRNLALLDDVRLKSSQLDTVIKTFEQIHDGSPLNVKHQLLKVVQHIFQYHVLLTDYLNNLCPDSAEYEDTQAALLIISELTDRANDSIQQGENLQKLVHIEHSVKGQSNLLQPGRKFLKEGTLMKVSGKNRHPRHLFLMNDVLLYTYPQKDGKYRLKNTLAVSDMKVSRPATKKAQNVLKIEYADNCLTLSASSCSERDEWYSCISRSIPEDHGAHSGGGFHNSVELRERLGMSLGERPPTLVPVSHVMMCMSCGCDFKLTLRRHHCHACGKIICRNCSRNKYPLKYLKDRLAKVCDGCYTELKSRERPISISFPTSSSRFSGSAFSSVFHYSSFKRQKKIPSALLEVAASGEGSSINGYLSRCKRGKRHWKKLWFVIKGKVLYTYTANEDKVATESMPLLGFTIVPEKEERNMDAVFHLYHKQTLFYSFRAEDNNSAQRWIEAMEEASIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQC9</accession>
    <name>A0A6I9YQC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MORC family CW-type zinc finger protein 2A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106552688</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926497.1">
        <property type="nucleotide sequence ID" value="XM_014071022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQC9"/>
    <dbReference type="GeneID" id="106552688"/>
    <dbReference type="KEGG" id="tsr:106552688"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23337:SF7">
        <property type="entry name" value="ATPASE MORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23337">
        <property type="entry name" value="ZINC FINGER CW-TYPE COILED-COIL DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013926497.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="14579" checksum="C9BA8501D3C77E9B" modified="2020-10-07" version="1">MGTPWSRNGELNQIVGLIKPSALCEALEQKQKVPLSFLKKEKTQEDKQKELTDKIRQQQEKLEALQKTTPVRSHADLKKLPLEVTAQPMLEVRPLLPLLFTELPSSVRPEVIQFSPLLTTAVGDQDVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR02</accession>
    <name>A0A6I9YR02_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 880-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106552345</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926070.1">
        <property type="nucleotide sequence ID" value="XM_014070595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR02"/>
    <dbReference type="GeneID" id="106552345"/>
    <dbReference type="KEGG" id="tsr:106552345"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002315">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF295">
        <property type="entry name" value="AZ2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013926070.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="27811" checksum="3F658D73582610F1" modified="2020-10-07" version="1">ASLPEEKPNEKRKYKCNVCGKFFSYKSSLKIHLRIHTGEKPYKCLECGKSFIRSDLLASHQRIHTGEKPYQCPECEKRFAEKSKLTNHYRIHTGERPYRCGQCDKRFVRAHHLVKHQSSVHQAGAKLYSCRECGQSFGHAQAFLSHQASHANGKRHQRCTECLKTFARRKYLLEHQRMHTGENPYTCPHCGKHFRYKQSLKQHLRMHREQPPPAPAVPPSLPGASSLEKDPLFETNPPSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR97</accession>
    <name>A0A6I9YR97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ANXA4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926160.1">
        <property type="nucleotide sequence ID" value="XM_014070685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR97"/>
    <dbReference type="GeneID" id="106552420"/>
    <dbReference type="KEGG" id="tsr:106552420"/>
    <dbReference type="CTD" id="307"/>
    <dbReference type="OrthoDB" id="37886at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004859">
        <property type="term" value="F:phospholipase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000005">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002390">
        <property type="entry name" value="ANX3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF25">
        <property type="entry name" value="ANNEXIN A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00199">
        <property type="entry name" value="ANNEXINIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926160.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="35733" checksum="344A70D15DD95A63" modified="2020-10-07" version="1">MTSTWVGSRGTIQDSAGFNASKDAEALKKAMEGLGTDETTVIEILTGRSNAQRQLIAAEYKSYTGKELKDALKEETSGDFGHVLCALVTPPAEFDAEEVNRAVRGIGTNEMTLIEIAASRTSKQIRETAQVYYTIYRKILSEDVGWDTSGDTKRTFLALLDAKRDENPQAHEEVAKRDAQLLYSAGEKRWGTDETKFIEILSTSSFAQLKLTFQEYQKLSGHRMEDTVLNETSGDFAQLLLAVVKCANNAPAFFAEKLNKAIKGPGTDEITVTRILVSRSEIDLLDIRHEYKNLFGESLYSAISSDSSGDYRRTLLKIIGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT30</accession>
    <name>A0A6I9YT30_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106553192</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 10">Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 43S pre-initiation complex (43S PIC), which is composed of the 40S ribosomal subunit, EIF1, eIF1A (EIF1AX), eIF3 complex, EIF5 and eIF2-GTP-initiator tRNA complex (eIF2 ternary complex). Interacts with EIF5; this interaction contributes to the maintenance of EIF1 within the open 43S PIC. Interacts through its C-terminal domain (CTD) with the CTD of EIF5B; from the location of the start codon by the 43S complex until the formation of the 80S complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927114.1">
        <property type="nucleotide sequence ID" value="XM_014071639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT30"/>
    <dbReference type="SMR" id="A0A6I9YT30"/>
    <dbReference type="GeneID" id="106553192"/>
    <dbReference type="KEGG" id="tsr:106553192"/>
    <dbReference type="OrthoDB" id="274995at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000071">
        <property type="entry name" value="Eukaryotic translation initiation factor 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="12">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="114"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013927114.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16442" checksum="1DE91334F191BEC5" modified="2020-10-07" version="1">MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEALCFDGVKRLCHIRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDTFGPGDDDEIQFDDIGDDDEDIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWB2</accession>
    <name>A0A6I9YWB2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inverted formin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928938.1">
        <property type="nucleotide sequence ID" value="XM_014073463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWB2"/>
    <dbReference type="GeneID" id="106554731"/>
    <dbReference type="KEGG" id="tsr:106554731"/>
    <dbReference type="OrthoDB" id="26518at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.150">
        <property type="entry name" value="Formin, FH3 diaphanous domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF5">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06346">
        <property type="entry name" value="Drf_FH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="424"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928938.1"/>
        </source>
    </evidence>
    <sequence length="581" mass="62862" checksum="1D4DD3F4E96F52C9" modified="2020-10-07" version="1">MLATSVWCVYVSYLDIEDEDLLIQCETFEEAKTEDDEELLRICDGIDMNSHQDVFSSLFNKVSSSPVSVQLLSILQSLLHLEPSHRSSLLLWESLEILVSRAILLANDIQDNSVEEVMDRLLSVKQHRKKRDLGSRRAAKKVNESTQTNEDLGQFPNITQSSPPRTCSSNNPLNGEPQNVSKVASSCFLVSSSSPEWNLSSAAPVPPPPPPPPPPLPGTLDMLPQQPSPLSGIQMPASVPTLHDVVTIPPPPPLPGMTSILPPPPLPSMTGIPSPPPLPTMIGAHSLPQNSAMPPPPPPLPGAVGIPPPPPLPGAVGIPPPPPLPGMVGIPPPPPLPGMVGIPPPPPLPGMVGIPPPLPGAAGVPPPPPPLPGMPPPPPPLPGMEGLPPPPPLPGIPPPPPLGFNVEEIVPAWASSLGSAAPPPKRRKMPTLRMKKLNWQKLPSNVVRESHSMWASTTRTSEEYIEPDYSKIEQLFCFPQTKPKSKEVAPVKTEPKEITFLDSKKSLNLNIFLKQFKCTNEEVVKMIQEGDRTKFDVEVLKQLLKLLPEKHEIENLKAFKEEKEKLSNADQFYLLLLGVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWF9</accession>
    <name>A0A6I9YWF9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribonuclease P protein subunit p29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">POP4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928632.1">
        <property type="nucleotide sequence ID" value="XM_014073157.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554482"/>
    <dbReference type="KEGG" id="tsr:106554482"/>
    <dbReference type="CTD" id="10775"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928632.1"/>
        </source>
    </evidence>
    <sequence length="99" mass="11385" checksum="EC1059686EF65354" modified="2020-10-07" version="1">LPEKVLYKQLPESTAKLVNLQPQGSNKAEVFVNAFLKRSMPGKSKEDIEKHFDRKAVVLEHPQPRSNRQRSKRRKGLSAKQRREXFCTILPLESGTFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWU4</accession>
    <name>A0A6I9YWU4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Multifunctional protein ADE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PAICS</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928441.1">
        <property type="nucleotide sequence ID" value="XM_014072966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWU4"/>
    <dbReference type="GeneID" id="106554316"/>
    <dbReference type="KEGG" id="tsr:106554316"/>
    <dbReference type="CTD" id="10606"/>
    <dbReference type="OrthoDB" id="9991235at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01416">
        <property type="entry name" value="SAICAR_synt_Ade5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000183">
        <property type="entry name" value="Probable multifunctional protein ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000020">
        <property type="entry name" value="Probable multifunctional protein ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000006">
        <property type="entry name" value="Probable multifunctional protein ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02045">
        <property type="entry name" value="PurE_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033626">
        <property type="entry name" value="PurE_classII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050089">
        <property type="entry name" value="SAICAR_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599:SF11">
        <property type="entry name" value="BIFUNCTIONAL PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE_PHOSPHORIBOSYLAMINOIMIDAZOLE SUCCINOCARBOXAMIDE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599">
        <property type="entry name" value="MULTIFUNCTIONAL PROTEIN ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="13">
        <location>
            <begin position="235"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013928441.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="43862" checksum="E87A989A1448B6DD" modified="2020-10-07" version="1">MQSKDQITAGNAARKNIMEGKAAISNTTTSCVFQLLQESGIKTAFVRKYSDTAFMAAHCEMIPIEWVCRRVATGSFLKRNPGVEEGYKFYPPKIEMFYKDDAANDPQWSEEQIIEVKFKLAGLEIGQTEVDIMAQSTRAIFEILEKAWQPQNCTLVDLKVEFGVNIATKEIVLADVIDNDSWRLWPSGDRKQQKDKQSYRDLKEVTPEALQVVKRNFEWVAERVELLLKPQTTQGRVVVLMGSTSDLGHCEKIKKACGSYGVPCELRVTSAHKGPDETLRIKAEYEGDGIPTVFVAVAGRSNGLGPVLSGNTAYPVVNCPPLSADWGAQDIWSSLRMPTGLGCSTILSPEAAAQFAAQIFGLNDHCVWAKLRSNILNTWISLKQADKKMREYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXM6</accession>
    <name>A0A6I9YXM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transportin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929052.1">
        <property type="nucleotide sequence ID" value="XM_014073577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXM6"/>
    <dbReference type="GeneID" id="106554843"/>
    <dbReference type="KEGG" id="tsr:106554843"/>
    <dbReference type="OrthoDB" id="435593at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF42">
        <property type="entry name" value="TRANSPORTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="1">
        <location>
            <begin position="110"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013929052.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="57295" checksum="178E68E13085ABDF" modified="2020-10-07" version="1">MDPVGPGGGAGGKPNLQMVYQAVQALYHDPDPSGKERASLWLGELQRSVHAWEISDHLLQIHQDVESCYFAAQTMKMKIQTSFYELPTDSHVSLRDSLLSHIQNLKDMSPVIVTQLALAIADLALQMASWKGCVHTLVEKYNNDVTSLPFLLEILTVLPEEVHSRSLRIGANRRTEIIEDLAFYSSTVVSLLMTCVEKAGSDEKMLIKIFRCLGSWFNLGVLDSTFMANSKLLSLLFEVLVRVLGGPTPPTPSQASEGQLGHSSAEKEDAVAGVGTWGWEQSHGGRSGTISPFMSPHATEGIPEETDDFGEFRMRVSDLVKDLIFLVGSVECFAQLYATLKEGNPPWEVTEAVLFIMASIAKSVDQENNPTLVEVLDGVVRLPEAVHTAVRYTSIELVGEMSEVVDRNPHFLDPVLGYLMKGLCEKALASIAAKAIHNICSVCRDHMAQHFTGLLEIARSLDSFMFSPEAAVGLLKGTALVLARLPLEKISPCLSELCAVQVMALKKIPTDPSGGKNQANLSAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY55</accession>
    <name>A0A6I9YY55_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Death-inducer obliterator 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106555001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929237.1">
        <property type="nucleotide sequence ID" value="XM_014073762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY55"/>
    <dbReference type="GeneID" id="106555001"/>
    <dbReference type="KEGG" id="tsr:106555001"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013929237.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="23340" checksum="B4A35E652B073403" modified="2020-10-07" version="1">MIMKKTPVATIKTEANAQTVGKEPTSESSTPSWASDHNYNAVKPEQTPAISPALLFKSHKEEQKVEDKPGEPPMKLKKTVGPTSVVEKHVTSQARSVVLKKPLPFPSTSLIKAPVKHSAADFKGVIPKKSSLATGSPASGGLSSSRPPLSSYGAHPMPKKPFPPSSAGAGLKRPVMSSASSSTTPLPPQAKLAASAGQSQPNSQIRQNIRRSLKEILWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZX8</accession>
    <name>A0A6I9YZX8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agrin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106555517</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929862.1">
        <property type="nucleotide sequence ID" value="XM_014074387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZX8"/>
    <dbReference type="GeneID" id="106555517"/>
    <dbReference type="KEGG" id="tsr:106555517"/>
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    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="F:laminin binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="agrin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000018">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004850">
        <property type="entry name" value="NtA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF78">
        <property type="entry name" value="AGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03146">
        <property type="entry name" value="NtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51121">
        <property type="entry name" value="NTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NtA" evidence="3">
        <location>
            <begin position="2"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="4">
        <location>
            <begin position="189"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013929862.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="27629" checksum="041C3CF4A0117A98" modified="2020-10-07" version="1">MGPVSQLHLIHKNRGKGGVLDGSLNLPKLRDTFGDNWLDLQTIASRCSRNRQGIANGFLRGKVRVWRYLKGKEVVTHEILLDGGNKVMIGGFGDPLICDNQVSTGDTRIFFVNPAPHYMWPVHKNELMLNSSLMRITLRNLEEVEHCVEDKPVNYFPQAVPQDVCRGMLCGFGAVCEKNPTDSSQGVCVCKKAACPSVVAPVCGSDYSTYSNECELEKAQCNQQRRIKVMSKGACGSKDPCTEVVCSFGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0A6</accession>
    <name>A0A6I9Z0A6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin-22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Pituitary and brain cadherin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CDH22</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. PB-cadherins may have a role in the morphological organization of pituitary gland and brain tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Cell membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929724.1">
        <property type="nucleotide sequence ID" value="XM_014074249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0A6"/>
    <dbReference type="GeneID" id="106555400"/>
    <dbReference type="KEGG" id="tsr:106555400"/>
    <dbReference type="CTD" id="64405"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000007">
        <property type="entry name" value="Cadherin 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF311">
        <property type="entry name" value="CADHERIN-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 15 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026831055" description="Cadherin-22" evidence="19">
        <location>
            <begin position="31"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="76"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="157"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="266"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="381"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="603"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013929724.1"/>
        </source>
    </evidence>
    <sequence length="717" mass="77834" checksum="1D46C9136EB48F1A" modified="2020-10-07" version="1">MVPPHPPRRGLLLLLLCLLVGPDRRPGASGAPGQGAPGDVSLPGSGRVKRGWVWNQFFVVEEYTGTEPLYVGKIHSDSDEGDGSIKYTISGEGAGTIFLIDELTGDIHATERLDRERKTFYTLRAQARDRQTDQLLEPESEFVIKVQDINDSEPRFLGGPYIGSVAELSPIGTSVLTVLASDADDPSYGSSARVAYSVLEGEQHFTVDSKTGVIRTAVANLDRETQDRYEVVVRATDMAGQLGGLSGSTTVTIVITDVNDNPPRFPQKMYQFSMVETAPVGMLVGRVRAEDADVGENTDMAYQIKEEGARGPFRVATDSNTQEALISLQQPLNFEAQAVHTVVLEALNKFAEPRFMGLDTFRDQTIVLVSVLDADEPPEFRPPSGPLEVQEDALVGSLVGVVTAVDPDAASRPVRYALEPPEDTGQIFDIDAQTGAILTRQALDRETAGWHNITILAMEADDTAGLHTQHLGFNRQEQDLFLLPILVVDSGPPALSSTGTLTVRICGCDNTGTIQSCNATAFVVSPSLSPGALIALLVCLLILVVLVLLILALKRHQKGHLPSEEDEDMRDNVIKYNDEGGGEQDTQAYDMSALRSLYEFGETKEGEEKGAPPPPPSELHSLPQWRQAPDLDFSLFRDYIRRKVGQADGDPSVPPYDSFQTYAFEGADSPLASLSSIASRSTGSEQDFSYLSGWGPRFRQLAALYAGPRAEEGNRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z172</accession>
    <name>A0A6I9Z172_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein KIAA1107 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KIAA1107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930049.1">
        <property type="nucleotide sequence ID" value="XM_014074574.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555681"/>
    <dbReference type="KEGG" id="tsr:106555681"/>
    <dbReference type="CTD" id="100624686"/>
    <dbReference type="OrthoDB" id="409642at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18490">
        <property type="entry name" value="BACK_BTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18285">
        <property type="entry name" value="BTB1_POZ_BTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18286">
        <property type="entry name" value="BTB2_POZ_BTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043225">
        <property type="entry name" value="BACK_BTBD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040121">
        <property type="entry name" value="BTBD8_BTB_POZ_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027907">
        <property type="entry name" value="BTBD8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22427:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22427">
        <property type="entry name" value="GH15728P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26017">
        <property type="entry name" value="BACK_BTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15363">
        <property type="entry name" value="BTBD8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="57"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="230"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1129"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1320"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="692"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="743"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="759"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="779"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="835"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="893"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="915"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="952"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="988"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1130"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1168"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1191"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1235"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1350"/>
            <end position="1365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930049.1"/>
        </source>
    </evidence>
    <sequence length="1768" mass="196333" checksum="34A273C4D141804A" modified="2020-10-07" version="1">MARCGGDSISRGSGGPREESGRKSGERGSAERRRLKELVAEQLRRDMERLLGEEIQTDITFYVGNTLFKAHKAVLLARVPHFFLHVVGRHLNTCSSCEALKLQYLEPSEFKAFLQAAYSSDRIITEEEQEILNKKVSTPELAKENEELKVDSLHSDQKCTEQRLGNHEKTSDHHWILCKRAVETECAAPSFSTGGMPTEAIDAESEVTMSEIASGLGKDLLMLYQNSWFSDINIWIDGKPFEVHRAILCARSSYFYAMLNGSWAESSQEHITLQGIRHAEMTVILHFIYGAVLEFPKNVNAGCILSIADMYGLDGLREIAIYILKRDYCNFFQKPVPGKHQPVLECLAIAYSTRSEHLYDSCMKWLIQNFSRCWSEKNFANLSSELQNNCLTTLIDSLSPQNAARLLMETNRLLNSLPQVKWTETARTLAFRLQEECMAFMVTNFPEIIQSESFLALLQAQAMSSKPDLLDQVLEAVKKEVTVENSCCLLIAVDTLLSSSSVNEMGFLCRIQALRDKLWVFLLQSFFAVRHTEGWKLLKAADQERIQAAAVDKGDDRRPTNKPIFSSSQLSRCFGGSCALKQTAWEASKKENPCGDPSANQDKMKSDALGASGQTSATNRNSNNKALKHDDLKGKDSKKAVCKLMKESKIGEKMPSPKARAVIKPKVENNGNAKNETLLAKQDSDRPLPSSGHKNAGSSKMSKNQEGKTTGARPKVFAGISNVQIKAKPVKKSVGKESPSLVSTDTLSKSNSSSPDLRMSIDKDDSKEEKGDESKKQSALKTKSGTKITNGALSKKHVNELEANSPTNSVTKKCTGKGNNEHIPQAVVKKRANEAGSSVPQQKTKNIANATKNQGLQGDLSNPLKAALSPKQNEENGMTQDMTLTSLKKKAKSSQATSVKSTAKIVTTKTQTHSKKTEMTNNKEQKQKTVSVLKLPSGQKPPRSESLLPKNIHGEEQKNSDLKLEHPSLEDQPCGDPKFNPEDKNDSETSLPELQRQNTPILGENIPLEEMACKRDLGSSCMSERNMSMKHDEAAKQIHKNEDDKSPINKKMEQCTLELHPIQCRESVARGNINQNATHLTLNEMEKCKTLPESVACQNSLETLRKNESHLVQGKSVVSFDTLEAEKDISQVNGSSDGSNSEQEEKKSSTLKTFLIDSESADELSDKSALTDSQLATVESEPASKSYVEQAAEKCSSKDTDTTETPESHENSEAPFTDQWNISSSRLDPKESPESDTGSATTSSDDIKPRSEDYDAGGSQDDEGSNERGISKCSTMLCHDFLGRSSSDTSTPEELKMYDTSLRIEVKMKKDNSELFRVISTSDEEIPRKKPETTWLRQGGERQSRSSGGNTNFATTQFPQEADQVSSSADETEEEKSENENAAEKMPPPEVPVQQFHGIVNLAFDDATENDIESQEFSATKNFKRSVLLSVDECEELGSDDGVEVHTPLQCTLDAATPSDVFDSVSHEPHSKTFYSRYSLDIEDGFLDCKGDDKNEKPFVDPQDSDQTLKDTLVTSVTEQTCMERAEKIGDGQLSAEVKCEGSNGFQCNKLLDNDTKTQGRPCHLDLHQRDNTDLQNSSTKPVDPYKTHFLAQEDTMKESASPEYTDTALLAGDIDDCDRLTQTCMYEHRLPKTLSPIYEMDVGEAFEQSMESEVNFLDMDFEDHQFAEQDWTLLRQLLSDQESNLDIKNSVPEDLNLAQYLINQTLFLARDTSQPQGKAQFDTFSKWTELISPLDDSSASITVASFSSEDCSSPHGEWTILELETHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z249</accession>
    <name>A0A6I9Z249_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serrate RNA effector molecule homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106556673</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931128.1">
        <property type="nucleotide sequence ID" value="XM_014075653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z249"/>
    <dbReference type="GeneID" id="106556673"/>
    <dbReference type="KEGG" id="tsr:106556673"/>
    <dbReference type="OrthoDB" id="342064at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007042">
        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
        <property type="entry name" value="ARS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERRATE/Ars2 C-terminal" evidence="5">
        <location>
            <begin position="199"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013931128.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="47565" checksum="0B2500241901B836" modified="2020-10-07" version="1">MRVALSDPQPERRFFRRGWVTFDRTVNIKEICWNLQNIRLRDCELSPGVNRDLTRRVRNINGMTQHKQILRNDIKLAAKLIHAMDDRTQLWGAGEAQLPSEVSNLPSQNPILKNITDYLIEEVSAEEEELLGRSGEPSEETPKEGNPAEITVERDEKLVKVLDKLLLYLRIVHSVDYYNTSEYLNEDEMPNRCGIIHVRGPIPPNRVTHREVAEWQKTFEEKLMPLFSIRESLSEEEALKMGKKDPEQEVENFVTSNTLELGKDKWQCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVSFFNNFLMDAKRPALPEFKLNQPPVPGQGLAPGLPYPPQAPQGLMPYGQPRPPILGYGGGPPYPPAPYAAGRGNYDTFRGQAGYLNKPRNRMVRGDPRAIVEYRDLDAPEDVDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2C7</accession>
    <name>A0A6I9Z2C7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kelch-like protein 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">KLHL15</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Dimerization does not affect PPP2R5B-binding, but is required for its proteasomal degradation. Interacts with CUL3. Directly interacts with PPP2R5B; this interaction leads to PPP2R5B proteasomal degradation. Interacts with RBBP8/CtIP; this interaction leads to RBBP8 proteasomal degradation. Interacts with PACMP micropeptide; interaction prevents ubiquitination and degradation of RBBP8/CtIP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931197.1">
        <property type="nucleotide sequence ID" value="XM_014075722.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931206.1">
        <property type="nucleotide sequence ID" value="XM_014075731.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931213.1">
        <property type="nucleotide sequence ID" value="XM_014075738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2C7"/>
    <dbReference type="GeneID" id="106556738"/>
    <dbReference type="KEGG" id="tsr:106556738"/>
    <dbReference type="CTD" id="80311"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18454">
        <property type="entry name" value="BACK_KLHL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18244">
        <property type="entry name" value="BTB_POZ_KLHL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000087">
        <property type="entry name" value="Kelch-like family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000009">
        <property type="entry name" value="Kelch-like protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000014">
        <property type="entry name" value="Kelch-like protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030597">
        <property type="entry name" value="BTB_POZ_KLHL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047030">
        <property type="entry name" value="KLHL15_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF12">
        <property type="entry name" value="KELCH-LIKE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="31"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013931206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013931213.1"/>
        </source>
    </evidence>
    <sequence length="604" mass="69682" checksum="DBB8B943CA0387C5" modified="2020-10-07" version="1">MAGDVEGFSSSIHDTSISAGFRTLYEEGLLLDVTLVIEDHQFQAHKALLATQSDYFRIMFTADMRERDQDKIHLKGLTATGFSHVLQFMYYGTIELSMSTVHEILQAAMYVQLIEVVKFCCSFLLAKICLENCAEIMRLLDDFGVNIEGVREKLDSFLLENFVPLMARPDFLSCLSFEKLMTYLDNDHLSRFPEIELYEAVQTWLRHDRRRWRHTDTIIQNIRFCLMTPSSVFEKVKTSEFYRYSRQLRHEVEQAMNYFHSVHQQPLMEIKSNRIRSAKPQTAVFRGMIGHSMVNSKILLLHKPRVWWELEGPQVPLRPDCLAIVNNFVFLLGGEELGPDGEFHASSKVFRYDPRQNSWLRMADMSVPRSEFAVGVIGRYIYAVAGRTRDETFYSTERYDITEDKWEFVDPYPVNKYGHEGTVLGSKLYITGGITSSSTSKQVCVFDPSKEGTVEQRTRRTQVVTNCWENKCKMNYARCFHKMLSYNGKLYVFGGVCVILRASFESQGCPSTEVYDPDTDQWTILASMPIGRSGHGVAVLDKQIMVLGGLCYNGHYSDSILTFDPEDNKWKEDEYPRMPCKLDGLQVCSLHFPEYVLEHVRRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2R2</accession>
    <name>A0A6I9Z2R2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 652-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106556077</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930519.1">
        <property type="nucleotide sequence ID" value="XM_014075044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2R2"/>
    <dbReference type="GeneID" id="106556077"/>
    <dbReference type="KEGG" id="tsr:106556077"/>
    <dbReference type="OrthoDB" id="7327383at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000166">
        <property type="entry name" value="Zinc finger and BTB domain-containing 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000658">
        <property type="entry name" value="Zinc finger protein 652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001755">
        <property type="entry name" value="Zinc finger protein 989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="124"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013930519.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="23138" checksum="67E16122B3968FE7" modified="2020-10-07" version="1">MSIHIGHKQFMCQWSGKAFNMKQYFDEHMKTHTGEKPFICEICGKSFTSRPNMKRHRRTHTGEKPYPCDVCGQRFRFSNMLKAHKEKCFRVTSPVNVPPSAQISLATTSSATTVSSVANTPNPLAPTSAINMNPASTLPPRPIGHPYSHLHLHSHHQHHLPVPVPPVPHLPPPPALFKSEPLNHRGQGEDSFLRHLAEKNSSVQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2S3</accession>
    <name>A0A6I9Z2S3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106556469</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013930937.1">
        <property type="nucleotide sequence ID" value="XM_014075462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2S3"/>
    <dbReference type="GeneID" id="106556469"/>
    <dbReference type="KEGG" id="tsr:106556469"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000096">
        <property type="entry name" value="KRAS proto-oncogene, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013930937.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="21315" checksum="09B2C7D8770AC533" modified="2020-10-07" version="1">MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSEDVPMVLVGNKCDLPTRTVDTKQAHEVAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKRLNSNEDGNQGCMGLSCLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z387</accession>
    <name>A0A6I9Z387_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="34">Fatty-acid amide hydrolase 1</fullName>
            <ecNumber evidence="3">3.5.1.99</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="37">Anandamide amidohydrolase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="35">Fatty acid ester hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="36">Oleamide hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="42" type="primary">LOC106556225</name>
    </gene>
    <organism evidence="41 42">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="42" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="42">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(11Z)-eicosenamide + H2O = (11Z)-eicosenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63120"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32426"/>
            <dbReference type="ChEBI" id="CHEBI:146167"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:63121"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(11Z,14Z)-eicosadienamide + H2O = (11Z,14Z)-eicosadienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63004"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:77220"/>
            <dbReference type="ChEBI" id="CHEBI:146165"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:63005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>(11Z,14Z,17Z)-eicosatrienamide + H2O = (11Z,14Z,17Z)-eicosatrienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63000"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:77223"/>
            <dbReference type="ChEBI" id="CHEBI:146164"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:63001"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>(15Z)-tetracosenamide + H2O = (15Z)-tetracosenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63028"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32392"/>
            <dbReference type="ChEBI" id="CHEBI:146166"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:63029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenamide + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:137830"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:63017"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(6Z)-octadecenamide + H2O = (6Z)-octadecenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63008"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32375"/>
            <dbReference type="ChEBI" id="CHEBI:146168"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:63009"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(8Z,11Z,14Z)-eicosatrienamide + H2O = (8Z,11Z,14Z)-eicosatrienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:62996"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:71589"/>
            <dbReference type="ChEBI" id="CHEBI:146163"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:62997"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(9Z)-octadecenamide + H2O = (9Z)-octadecenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:26506"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:116314"/>
            <dbReference type="EC" id="3.5.1.99"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:26507"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(9Z)-octadecenoate + glycine = N-(9Z-octadecenoyl)glycine + H2O</text>
            <dbReference type="Rhea" id="RHEA:51316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:133992"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:51318"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(9Z,12Z)-octadecadienamide + H2O = (9Z,12Z)-octadecadienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:82984"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:63021"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(9Z,12Z,15Z)-octadecatrienamide + H2O = (9Z,12Z,15Z)-octadecatrienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:62976"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32387"/>
            <dbReference type="ChEBI" id="CHEBI:142684"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:62977"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-O-methyl-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H2O = methanol + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63052"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:78033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:63053"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = glycerol + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:26133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-(15Z-tetracosenoyl)-ethanolamine + H2O = (15Z)-tetracosenoate + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:63144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32392"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:146187"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:63145"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>N-(15Z-tetracosenoyl)-taurine + H2O = (15Z)-tetracosenoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:63160"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32392"/>
            <dbReference type="ChEBI" id="CHEBI:146198"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:63161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:64108"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:59002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:64109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:64116"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:149697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + H2O = ethanolamine + (5Z,8Z,11Z,14Z)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:26136"/>
            <dbReference type="ChEBI" id="CHEBI:2700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="EC" id="3.5.1.99"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:26137"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N-(9Z-hexadecenoyl) ethanolamine + H2O = (9Z)-hexadecenoate + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:35563"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:71465"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:35564"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-(9Z-octadecenoyl) ethanolamine + H2O = ethanolamine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:45060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:71466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:45061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N-(9Z-octadecenoyl)-taurine + H2O = taurine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:63148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:146191"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:63149"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-docosanoyl-ethanolamine + H2O = docosanoate + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:63128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:23858"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:146186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:63129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N-docosanoyl-taurine + H2O = docosanoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:63156"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:23858"/>
            <dbReference type="ChEBI" id="CHEBI:146196"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:63157"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>N-octadecanoyl ethanolamine + H2O = octadecanoate + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:63124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:85299"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:63125"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N-tetracosanoyl-taurine + H2O = tetracosanoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:63140"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:132049"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:63141"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>N-tricosanoyl-taurine + H2O = tricosanoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:63164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:79007"/>
            <dbReference type="ChEBI" id="CHEBI:146197"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:63165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tetradecamide + H2O = tetradecanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:62992"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:137125"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:62993"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.99" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013930689.1">
        <property type="nucleotide sequence ID" value="XM_014075214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z387"/>
    <dbReference type="GeneID" id="106556225"/>
    <dbReference type="KEGG" id="tsr:106556225"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017064">
        <property type="term" value="F:fatty acid amide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1300.10:FF:000001">
        <property type="entry name" value="Fatty-acid amide hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052096">
        <property type="entry name" value="Endocannabinoid_amidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45847">
        <property type="entry name" value="FATTY ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45847:SF8">
        <property type="entry name" value="FATTY ACID AMIDE HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="39" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="39" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="39" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="39">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="40">
        <location>
            <begin position="82"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <begin position="172"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00052634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00052709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00052818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00052857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00052906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00073178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00077111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00077157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00077216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="RefSeq" id="XP_013930689.1"/>
        </source>
    </evidence>
    <sequence length="514" mass="56656" checksum="4C9A8DCBCE6876FF" modified="2020-10-07" version="1">MMQEKLRDILLQNGINPPIVLAVACGSVTVVVLWKWVIHREIQRKMKEARQWRNSSLEQMKKVAWKLKQKHYIKQVVFSLPKGHISTCGLVQYLDKVEVDDSVIVKVLKKQGAIPFVKTNIPQSMINYDCSNLIFGQTKNPLNHKKSPGGSSGGEGALIAGGGSVLGFGTDVAGSIRLPSSFCGLCGLKPTGSRLSTLGLASPVAGMKSVTATIGPMARDVDSLALCMKALLCDELFHLDPSVPPLPFREEIYTSSNLLRIGYYKDDGYFEPSPSMKRAVQETKNLLQAAGHTLIPFVPPRLNYVVDELFTKGIFSDGAANLLAKFKGDIVDPGLKSQINCYSIPTILKRILALILKPVYPRIARDLNALCGVGSAKHLWKQHAAVAAYREEFIAEWRKLKLDVILCPVLGPAFNEGYPGKLFAATSYTNLYNVLNFPAGVVPVTNVTQADEEELKQYEGHYADPWDKRLKEAVTDAVGLPVAVQCVALSWEEELCLRFMKEVETLTCKNRLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3U6</accession>
    <name>A0A6I9Z3U6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ARF GTPase-activating protein GIT2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GIT2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931322.1">
        <property type="nucleotide sequence ID" value="XM_014075847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3U6"/>
    <dbReference type="GeneID" id="106556836"/>
    <dbReference type="KEGG" id="tsr:106556836"/>
    <dbReference type="CTD" id="9815"/>
    <dbReference type="OrthoDB" id="5588096at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036465">
        <property type="term" value="P:synaptic vesicle recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000013">
        <property type="entry name" value="ARF GTPase-activating protein GIT1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000003">
        <property type="entry name" value="ARF GTPase-activating protein GIT2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000015">
        <property type="entry name" value="ARF GTPase-activating protein GIT2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047161">
        <property type="entry name" value="GIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032352">
        <property type="entry name" value="GIT1/2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097:SF4">
        <property type="entry name" value="ARF GTPASE-ACTIVATING PROTEIN GIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16559">
        <property type="entry name" value="GIT_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="11">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="166"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="377"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="465"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="496"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013931322.1"/>
        </source>
    </evidence>
    <sequence length="716" mass="79448" checksum="C81E5046031D2D46" modified="2020-10-07" version="1">MAKRLRSSDVCADCSAAEPSWASVNRGVLLCDECCSVHRSLGRHISQVRHLKHTPWPATLLQMVETLYNNGANSIWEHSLLDPASVMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVAAKTGQILQAELLAVYGADPGTPDSCGKTPVDYARQGGHLELAERLVEIQYELTDRLAFYLCGRKPDHKGRQHFIIPQMADSSLDVSELAKAAKKKLQLLNNHLFEELAMDVYDEVDRRETDAVWLATQNHSTLVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILGDAKRRQQGNPVPSATENVELILKAVNSQPGPGGHEADQPDYDSVASDEEADLESSSGKASRQKSLDSDLSDGPITAQEYLQVKNALVASEAKIQQLLKVNINLSDELRTMQKKLQSLQSENTSLRRQATPNTHPASAGSEYADVPSIRRPSLRSSRPMSMYETGSTQKPYLPLTEAAYPEEHIVPRLQLFHMSRLEKQSSLPEGDYDNTGPALELDETGLIRKARQRSVLGPEGKDPESHPSFPSTEDVIRKTEQITKNIQELLRAAQESKHESYIPCSERIHLAVTEMAALFPKKPPSELVRTPLRLLTSSAFRLHSECVKALPPESCSTADVQLVTQQVIQCAYDIAKAAKQLVTITTKENAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4M9</accession>
    <name>A0A6I9Z4M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cap-specific adenosine methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphorylated CTD-interacting factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PCIF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CAPAM family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.62" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013930815.1">
        <property type="nucleotide sequence ID" value="XM_014075340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4M9"/>
    <dbReference type="GeneID" id="106556340"/>
    <dbReference type="KEGG" id="tsr:106556340"/>
    <dbReference type="CTD" id="63935"/>
    <dbReference type="OrthoDB" id="193787at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016422">
        <property type="term" value="F:mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099122">
        <property type="term" value="F:RNA polymerase II C-terminal domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000020">
        <property type="entry name" value="Phosphorylated CTD-interacting factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000036">
        <property type="entry name" value="Phosphorylated CTD-interacting factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039881">
        <property type="entry name" value="PCIF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022035">
        <property type="entry name" value="PCIF1_WW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21727:SF0">
        <property type="entry name" value="MRNA (2'-O-METHYLADENOSINE-N(6)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21727">
        <property type="entry name" value="PHOSPHORYLATED CTD INTERACTING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12237">
        <property type="entry name" value="PCIF1_WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="WW" evidence="11">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="114"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="665"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="684"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013930815.1"/>
        </source>
    </evidence>
    <sequence length="703" mass="80004" checksum="8F7E538691F71704" modified="2020-10-07" version="1">MANENHGSSREEAALMSHSPSTSHQNQPSSPKAARLVQDLPDELVQAGWEKCWSKRESRPYYFNRFTNQSLWEMPLLGQHDVISDPLGLNAAPVPADGGAGEAAAAESRLWKRKLSEEGQPSSNLAKKPKMEVPGNPTAQPAPNISSTPGTPLLKAWGVSPEERQAALLRPTEVYWDLDIQTNAVIKQRAPADVLTPHPEVELLRSQLMLKLRQHYRELCQQREGIDPPRESFNRWMLERKVVDKGSDPLLPSNCEPVVSPSMFREIMNDIPIRLSRIKFREEAKRLLFKYAEAAKRLIESRSASPDSRKVVKWNVEDTFSWLRRDRSASKEDYMDRLEHLRKQCGPHVSAAAKDSVEGICSKIYHISLEYVKRIREKHLAILKEHSISAEVEPPDVQDRLVYCYPVRLAVPSPPLPSAEMHMESSLVCVRYKGEVQKVSRSYFSKLWLLYRYSCIDDSGFEHFLPRVWCLLRRYQMMFGVGLYEGTGLQGALPVHVFEALYKLFGVSFECFASPLNCYFKQYCSAFPDTDGYFGSRGPCLDFFPISGSFQANPPFCEELMDVMVTHFEKLLEASSEPLSFIVFIPEWRDPPPTALTRMEQSPFKRQQLILPAFDHEYRSGSQHLCKKEEMYYKAVHNTTVLFLQNNAGFSKWGPSPERLHELLTAYKHSGRAHPAASAASSAPDKEQEPVREQSTSRESTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4Y5</accession>
    <name>A0A6I9Z4Y5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acyltransferase PGAP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Post-GPI attachment to proteins factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PGAP2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the fatty acid remodeling steps of GPI-anchor maturation where the unsaturated acyl chain at sn-2 of inositol phosphate is replaced by a saturated stearoyl chain. May catalyze the second step of the fatty acid remodeling, by reacylating a lyso-GPI intermediate at sn-2 of inositol phosphate by a saturated chain. The fatty acid remodeling steps is critical for the integration of GPI-APs into lipid rafts.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PGAP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931269.1">
        <property type="nucleotide sequence ID" value="XM_014075794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4Y5"/>
    <dbReference type="GeneID" id="106556786"/>
    <dbReference type="KEGG" id="tsr:106556786"/>
    <dbReference type="CTD" id="27315"/>
    <dbReference type="OrthoDB" id="68581at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039545">
        <property type="entry name" value="PGAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12892">
        <property type="entry name" value="FGF RECEPTOR ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12892:SF11">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="12">
        <location>
            <begin position="21"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931269.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="29408" checksum="6435AF981060E364" modified="2020-10-07" version="1">MLPVALPLERERDGALLRLRFTWLAAGTVGCPLGGFLFCLAWSLLFHFHEATATHCGVPNYLPSLSAAIGAATPERYVWRLCIGLHSAPRLLVASAYWRHYRGCPCLHPRYSRLCHAVLALSLAENLGLLLLTYVSSAENHGFHQHAFGLFIVSALGHMFLTCLLWRLTKKYAISPEERKSYKWKWWLFLFNLLTFLMAVCCYFRHNLYCEAGVYTMFAFLEYLVVLSNMAFHMTAWWDFGNKELVVSSQLEDKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z8R9</accession>
    <name>A0A6I9Z8R9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribose-phosphate pyrophosphokinase 2</fullName>
            <ecNumber evidence="5">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphoribosyl pyrophosphate synthase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PRPS2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer. The active form is probably a hexamer composed of 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013932165.1">
        <property type="nucleotide sequence ID" value="XM_014076690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z8R9"/>
    <dbReference type="GeneID" id="106557431"/>
    <dbReference type="KEGG" id="tsr:106557431"/>
    <dbReference type="CTD" id="5634"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000011">
        <property type="entry name" value="Putative ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="17">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013932165.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="35367" checksum="75D05C18E48196F5" modified="2020-10-07" version="1">MPNIVLFSGSSHQDLSQRVADRLGLDLGKVVTKKFSNQETSVEIGESVRGEDVYIIQSGCGEINDNLMELLIMINACKIASSSRVTAVIPCFPYARQDKKDKDQGRWSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLQWIKENIVDWRNCVIVSPDAGGAKRVTSIADRLNVDFALIHKERKKANEVDRMVLVGDVTDRIAILVDDMADTCGTICHAADKLVSAGATKVYAILTHGIFSGPAISRINNAAFEAVVVTNTIPQEEKMKHCPKIQVIDISMILAEAIRRTHNGESVSYLFSHVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-09-12" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2U8BQE7</accession>
    <name>A0A2U8BQE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">G protein beta subunit 1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="10 11">Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 10 11" type="primary">GNB1</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2018" name="Mol. Biol. Evol." volume="35" first="1376" last="1389">
            <title>Shifts in Selective Pressures on Snake Phototransduction Genes Associated with Photoreceptor Transmutation and Dim-Light Ancestry.</title>
            <authorList>
                <person name="Schott R.K."/>
                <person name="Van Nynatten A."/>
                <person name="Card D.C."/>
                <person name="Castoe T.A."/>
                <person name="S W Chang B."/>
            </authorList>
            <dbReference type="PubMed" id="29800394"/>
            <dbReference type="DOI" id="10.1093/molbev/msy025"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="EMBL" id="MH011510">
        <property type="protein sequence ID" value="AWC68144.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912152.1">
        <property type="nucleotide sequence ID" value="XM_014056677.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912153.1">
        <property type="nucleotide sequence ID" value="XM_014056678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2U8BQE7"/>
    <dbReference type="GeneID" id="106541288"/>
    <dbReference type="KEGG" id="tsr:106541288"/>
    <dbReference type="CTD" id="2782"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="222"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AWC68144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013912152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912153.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="37264" checksum="4C2F89647279869B" modified="2018-09-12" version="1">MSELDQLRQEAEQLKNQIRDARKACADATLAQITANIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFVDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDSKCFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMVYSHDNIICGITSVAFSKSGRLLLAGYDDFNCNVWDTLKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WWF2</accession>
    <name>A0A6I9WWF2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Keratin, type I cytoskeletal 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytokeratin-14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Keratin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106537584</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907239.1">
        <property type="nucleotide sequence ID" value="XM_014051764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WWF2"/>
    <dbReference type="GeneID" id="106537584"/>
    <dbReference type="KEGG" id="tsr:106537584"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF368">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="4" id="KW-0416">Keratin</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="14">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013907239.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="23634" checksum="D4F46622BE22B197" modified="2020-10-07" version="1">MRCITSLDVASAKERIQKSEMRLLQSQMGGEITVEMDAAPGVDLTKVLAEMREQYEGLAEKNRRDAEQWFFSKTEELNREVAINTEQLQSDQSEVIELRRSLQGLEIDLQAQLSMKAALEGTLADIESRYSIQLAQIQGLIINVEEELATLRCDMERQNHEYKILLDVKTRLEQEIATYRRLLEGEETYISPQYSSAVEGKDLPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WWS3</accession>
    <name>A0A6I9WWS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">POU1F1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907374.1">
        <property type="nucleotide sequence ID" value="XM_014051899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WWS3"/>
    <dbReference type="GeneID" id="106537706"/>
    <dbReference type="KEGG" id="tsr:106537706"/>
    <dbReference type="CTD" id="5449"/>
    <dbReference type="OrthoDB" id="6358449at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000150">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF84">
        <property type="entry name" value="NETRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="13">
        <location>
            <begin position="174"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="262"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="264"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013907374.1"/>
        </source>
    </evidence>
    <sequence length="341" mass="37872" checksum="1A4C407D73183D4F" modified="2020-10-07" version="1">MSCQVFSSADTFIPLTSDSSPTLPLIMHHSAAECLPVSNHATNVVSTGTVSLIIKLLFLSVCHFTGFISSPSRPSLIGIKPTAPDVLAASLSQSRILQTCSMSHPNVVNGVGTLQGGLTPCLYKFPEHALSASSCALGHSFTSMHPSLLGADPTTDFKQEFRKKAKSLDEPIDMDSPEIRELEKFANEFKLRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKSILTKWLEEAEQVGALYNEKVGMNERKRKRRTTISIAAKEALESHFAEQSKPSSQEIMRMAEGLNLEKEVVRVWFCNRRQREKRVKTSLHQNTFNSIIKDHQEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYB4</accession>
    <name>A0A6I9WYB4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Probable C-mannosyltransferase DPY19L1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">LOC106537519</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907150.1">
        <property type="nucleotide sequence ID" value="XM_014051675.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907151.1">
        <property type="nucleotide sequence ID" value="XM_014051676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYB4"/>
    <dbReference type="GeneID" id="106537519"/>
    <dbReference type="KEGG" id="tsr:106537519"/>
    <dbReference type="OrthoDB" id="6019623at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018732">
        <property type="entry name" value="Dpy-19/Dpy-19-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31488:SF1">
        <property type="entry name" value="C-MANNOSYLTRANSFERASE DPY19L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31488">
        <property type="entry name" value="DPY-19-LIKE 1, LIKE (H. SAPIENS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10034">
        <property type="entry name" value="Dpy19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013907150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013907151.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="23800" checksum="1B24680826979227" modified="2020-10-07" version="1">MRLKLFLTPHMCIMASLICSKQLFGWIFHKIQPGTLVLAILAMMAFEGIANLQKEWSIVGEFSNLPQEELLLWIKANTKPDAVFAGAMPTMASVKLSAQRPIVNHPHYEDAGLRARTKIVYSMYSRKPAKEVKRELLKMGVNYYILEDSWCLRRTKPGCSMPEIWDIEDLTNAGKIPLCNLMCKDSRPYFRTVFNNNIFKILEVTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0M2</accession>
    <name>A0A6I9X0M2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ankyrin repeat and sterile alpha motif domain-containing protein 1B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106538049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907901.1">
        <property type="nucleotide sequence ID" value="XM_014052426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0M2"/>
    <dbReference type="GeneID" id="106538049"/>
    <dbReference type="KEGG" id="tsr:106538049"/>
    <dbReference type="OrthoDB" id="10039052at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09499">
        <property type="entry name" value="SAM_AIDA1AB-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000490">
        <property type="entry name" value="ANKS1B isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000016">
        <property type="entry name" value="Ankyrin repeat and sterile alpha motif domain-containing protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033635">
        <property type="entry name" value="ANKS1/Caskin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041880">
        <property type="entry name" value="SAM_ANKS1_repeat1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF3">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="193"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="225"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="809"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="880"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="366"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="739"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="750"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013907901.1"/>
        </source>
    </evidence>
    <sequence length="924" mass="101381" checksum="3CBFAC9226B38555" modified="2020-10-07" version="1">MGKDQELLEAARTGNVALVEKLLAGRKGGLLGSGSGPIPLSNLLSIWRGPNINCTDSSGYTALHHAALNGHKDVVIKLLQYEASTNVADNKGYFPIHLAAWKGDVEIVKILIHHGPSHSRVNEQNNENETALHCAAQYGHSEVVALLLEELTDPTIRNNKLETPLDLAALYGRLRVVKMIINAYPNLMSCNTRKHTPLHLAARNGHKAVVQELLEAGMDVSCQTEKGSALHEAALFGKVDVVRLLLETGIDANIKDNLGRTVLDILKEHPSQQSLQIAALLQDYIEKGNSEVPEESIEEYLVSDHHSSVLSPEDSPIQTSKSEAVTGELSKLLDEIKLCQEKDYLEDMSHTISDDYLDDANKVSEEETVANGNRDKSNIRSSSTNLEQTEEEEEGGTENSCGPTGLWEALTPCNGCRNLGFSSLTQETFPKKRSATLEAVPSVSSETFPLENENGLCDFLDTTVTKKPCSLEITRGVSSKIDNAPQVAITAPGTGNHRNSSTGPTPDCSPPSPDTALKNIVKVIRPQAKQRTSIVSAFEFNRLNQSHDYFEINSCVRYASFNSSSPVSPANCSTASVEDSIEENNIPGTQQTSIDSDHLEAHPFEQFAGLLHGSSPACDPPENPFQLYRKICPKEDLNQKISLNLTILNPQHSLQENSAVLLIKKKKPPILNRTIFPSKNILGEANTLAGITRTGDQWVVNAAGIVERACTLGRIRSLPKTLLDIHLSKYISKSDSNLVSYPSNDHKARQNWNESSAIQETSRRNSERTPSFTSEWEEIDKIMSSIDAGITTGLEEITDGTATPRCPVQTVGQWLENIGLPQYENHLLANGFDNVQFMGSNVMEDQDLLEIGILNSGHRQRILQAIQLLPKMKQTGSDGYNPTSVAEWLDSLELGDYIKPFLINGYTSMDLAKKIWDIELINVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0W4</accession>
    <name>A0A6I9X0W4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LOW QUALITY PROTEIN: adenylate cyclase type 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ADCY10</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907437.1">
        <property type="nucleotide sequence ID" value="XM_014051962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0W4"/>
    <dbReference type="GeneID" id="106537742"/>
    <dbReference type="KEGG" id="tsr:106537742"/>
    <dbReference type="CTD" id="55811"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000017">
        <property type="entry name" value="Adenylate cyclase type 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000021">
        <property type="entry name" value="Adenylate cyclase type 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16305:SF28">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16305">
        <property type="entry name" value="TESTICULAR SOLUBLE ADENYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="42"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="502"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013907437.1"/>
        </source>
    </evidence>
    <sequence length="1268" mass="143325" checksum="8277F0E03995745A" modified="2020-10-07" version="1">MTNKTDQIEISTLGKLAAHVPDIIVYGDFAQELPFIESFDGVLLFMDISGFTALTEKFSMNTSLDRGADELTQTLNHYMGDIVEGFVLLFPGDALLALWKVERCHLNEIITLALKCSFSIQQEYGVRDTEVGLELRVKIGVSAGHISKVVIGDNRRQHFVVTGRAVEEVRLAQNLAKASEIIMSPNCWELCDRNLIEVEKIKNERAVKVGTFLGKSPFCFLLHCNAVSLAQQAVLLASLVASCELHQQWVSTCTTIGLGAGQSRSLSCELTAAFNKLLWKTGCTFPNKTSACFIFSQPSYSNSIHLARVIDDGNSHGSSGMGTNSLLLVTCKEVKYIRNVPDFDTNEFFDRCAEYLNQNHICDSFEVLRIASVLGPDEELEKRLRKYVMKNVLKKIDDNQPVEYLSELRPVTVVFVNMQFEENANTNHVCKAIQDANDMIAGTILPVSGNINKVFMFDKGCTFLCLFGLPGDKQADESTNALDSAHKIHAFCSKELMKVKIVSIGVTAGPVFCGVVGHRVRHEYTVIGRKVNLAARMMMFYPGLVSCDALTYSKSKLPHYFFEELPLIEMKGVVNPGTVYQFLGITEKAFCYIHYLDFLDVSLIVWVVAMELTKINAWQNFFAVRTLMAMFLGVDVCKTYDARQYVILSKLRGILEEQYLCLLNNLFHVKAAEKEVIIFAIDEAQYIDKASWDFLDNLLKSVPIFIVMALSPLNHKGRILCSSAAQILNSPNTTFIRLRELSPSVIIQKACQDLGVVRIALAALDNMNPSEQMVVKCAAVIGVTFATDMLLYILPEWTKRKMYQTLAALVESRIFKSGSKRKEVNAMRRMLSKESSIRNSELKSQREESDLGSVISNRLELEETVMHCKIMEFCTPLLQEAAYELWLKNQKKALHFKCANYLKWLAHRCKCCGEGDFIPYHRYAVDGMLQKFYSQDYKIKTIKEEVLNEAALILVSETLKKLEASHQEVRELLVFGDYHIEYRNEYFLFCYSYLAFTYLNTAENLLRPSDYKHKLISQFEVSVLYSLKGEVSYNMGQITSAKKLIKKALSLLKKSFPITVVGAFCMFMVETSKHLSHQNKQTLWQISSGREKRLAALFQQSHCLSLLWQLFNLDVAGSRKTFTRLAALMQVNCAEESQDESQIISSYMDFSLCYQMMGCQHEWMKYELMAIKRSSHLQVVGGGLLTIAKLASSLSYMKLCLGNIPLAIQLGYRAHELYEQLKRPNLDATVLHDIFKALYLSTRLDLSLPICTHLQGYLGFLLEQNPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1C7</accession>
    <name>A0A6I9X1C7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">28S ribosomal protein S11, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MRPS11</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909059.1">
        <property type="nucleotide sequence ID" value="XM_014053584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1C7"/>
    <dbReference type="GeneID" id="106538939"/>
    <dbReference type="KEGG" id="tsr:106538939"/>
    <dbReference type="CTD" id="64963"/>
    <dbReference type="OrthoDB" id="1654884at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013909059.1"/>
        </source>
    </evidence>
    <sequence length="117" mass="12439" checksum="0A5756276CA3C153" modified="2020-10-07" version="1">MWISNQTLFLSSHDICSTHIQVFTAEKVPVVLLTCGYMGFKNAKKSTALAAQTTGIAAGNKAAKKGVYHVRVVVKGLGPGRLAAIQGLTLGGLEVISITDNTPVPHNGCRPRKARRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5N6</accession>
    <name>A0A6I9X5N6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106539311</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909561.1">
        <property type="nucleotide sequence ID" value="XM_014054086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5N6"/>
    <dbReference type="GeneID" id="106539311"/>
    <dbReference type="KEGG" id="tsr:106539311"/>
    <dbReference type="OrthoDB" id="413723at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000003">
        <property type="entry name" value="kinesin light chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF2">
        <property type="entry name" value="KINESIN LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
        <property type="entry name" value="KINESIN_LIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="264"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="306"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="168"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="108"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909561.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="46690" checksum="5D80D1E35DFC7CE6" modified="2020-10-07" version="1">MTVVTQVGAMATMLYPREEKLGKMSQDEIVLGTKAVVQGLETLRSEHHSLLAALLDTIGRLEQPPPQQQQPHEKGAAQEKALLLRKSLEAIELGLGEAQVIIALSSHLSAVESEKQKLRAQVRRLVQENQWLRDELAATQHKLQRSEQSVAQLEEEKKHLEFMNQIKKFDEDVSPSDEKNGEASKETLDDLFPNDDDQGQGQTHHSGESAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKYHPDVAKQLNNLALLCQNQGKYEEVQYYYQRALEIYESRLGSDNPNVAKTKNNLASSYIKQGKYKEAETLYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6W0</accession>
    <name>A0A6I9X6W0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="7">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Lysophospholipase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Lysophospholipase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC106537496</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1-(9Z-octadecenoyl)-2-acyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57875"/>
            <dbReference type="ChEBI" id="CHEBI:78421"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:41721"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013907128.1">
        <property type="nucleotide sequence ID" value="XM_014051653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6W0"/>
    <dbReference type="GeneID" id="106537496"/>
    <dbReference type="KEGG" id="tsr:106537496"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF22">
        <property type="entry name" value="ACYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="25">
        <location>
            <begin position="11"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00045255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013907128.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13181" checksum="7E688AB526943051" modified="2020-10-07" version="1">MCGNTMSAPLPAIVPAAKKATAAVIFLHGLGDTGHGWAEAFAGIRSPHVKYICPHAPVMPVSLNMNMAMPSWFDIFGLAPDSQEDEAGIKQASENVNTLIEQEVRNGIPSNRIILGGFSQVILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7V8</accession>
    <name>A0A6I9X7V8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein sel-1 homolog 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106540837</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013911574.1">
        <property type="nucleotide sequence ID" value="XM_014056099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7V8"/>
    <dbReference type="GeneID" id="106540837"/>
    <dbReference type="KEGG" id="tsr:106540837"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042756">
        <property type="entry name" value="Sel-1L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44444">
        <property type="entry name" value="PROTEIN SEL-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44444:SF1">
        <property type="entry name" value="PROTEIN SEL-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013911574.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="50106" checksum="03D7A29B4DD65F18" modified="2020-10-07" version="1">MLFWGQQGVGKNLRKAVEWYAKGALENEDPVSLFDYSIVLFKGQGVKRNRRLAFQLMKKASSKGFPQAVNGLGWYYHNFKRDYGKAAKYWLKAEAMGSPDASFNLGVLHLDGVYPGATGRNRTMAAGYFYKAAEKGHMEGTLHCAHFYITGNLPSFPRDPEKAVLWAKHVAEKNGYLGHVIQKGLRAYLELSWNEALLYYILSAETGIEVAQTNLAHICEEKPNLTNRYFSTDCVRKYYNFSISQSTAPSFAYLKVGDFYYYGAQNQTRDLNMSARMYAQAALMGDAQGFFNLALLLEEGYPIPSSLLDRLEIDSTAHSRNMSLHQELYERCWNYSNQDSVNPCSLALLYLRLRIWSNNIFHSDLIYYLGCFSPYLLVAFVIWRFWSLSAVARLDNSAVAEGGPLPRDGEPNQRRSAEREASPDPNGFEEDSINPQHSSSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8M2</accession>
    <name>A0A6I9X8M2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cytochrome P450 2G1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106541085</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911889.1">
        <property type="nucleotide sequence ID" value="XM_014056414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8M2"/>
    <dbReference type="GeneID" id="106541085"/>
    <dbReference type="KEGG" id="tsr:106541085"/>
    <dbReference type="OrthoDB" id="3934656at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008392">
        <property type="term" value="F:arachidonate epoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019373">
        <property type="term" value="P:epoxygenase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000001">
        <property type="entry name" value="Cytochrome P450, family 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008067">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP2A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF424">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01684">
        <property type="entry name" value="EP450ICYP2A"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013911889.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="56296" checksum="AF8E1901FB64A508" modified="2020-10-07" version="1">MELGGTVGIFLGICFSCLLLISAWKRMHKEGKLPPGPTPLPFLGNILQVKTSEPFKSFLALKEKYGPVFTVYLGPRRVVVLCGHDTVKEALVDQAEEFSGRGEIASIDRNFNGFGVAFANGERWKQLRRFSLTTLRNFGMGKRSIEERIQEEAQYLMEEIRKTKGQPFDPTFFLSRTVSNVISSVVFGSRFDYEDKTFLSLLQMINESFMEMSTPLAQLYDMYSCIMQYLPGRHNRVYYLVEDLKDFIAGKIKTNQATLDPSNPRDFIDCFLIQMEKEKGNPSTEFNLKNLVLTTLNLFFAGTETVSSTLRYGLLFLMKYPEVEEKIYKEIDQVIGPSRLPATEDRLKMPYTDAVVHEIQRVTDIVPMGVPHTVIRDTQFRGYLLPKGTDVFPLLGSALRDPKYFSDPENFNPGHFLDENGRFKKNEAFVPFSSGKRVCLGEAMARMELFLYFTTILQNFSLKSSLHPKDIDVSPKMSGFGNIPPVYQLSMLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8S0</accession>
    <name>A0A6I9X8S0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RalBP1-associated Eps domain-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">REPS2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910751.1">
        <property type="nucleotide sequence ID" value="XM_014055276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8S0"/>
    <dbReference type="GeneID" id="106540226"/>
    <dbReference type="KEGG" id="tsr:106540226"/>
    <dbReference type="CTD" id="9185"/>
    <dbReference type="OrthoDB" id="10045710at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF64">
        <property type="entry name" value="RALBP1-ASSOCIATED EPS DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="5">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="5">
        <location>
            <begin position="266"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="299"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="432"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="579"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="622"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="589"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013910751.1"/>
        </source>
    </evidence>
    <sequence length="670" mass="74136" checksum="197FEBE547ADC170" modified="2020-10-07" version="1">MEPEGTAFTGPAATGTPGFFLARNEQQYFSELFSLCGGGGQPTPEAGSRLHPPALGGSKVAELFRASQLPAEMLHQITDLCGAKHVGYFGRTQFYAALKLIAAAQSGLPVRIESIQNELPLPRFSALKSDTEIRYINLSEAHGAKFQSPHTTLEINCFRRSGEEEKQEEVKSPPMSPIVSHPASPSNYQKIPLGYGYNILRSGLEQQHGGSYEARHSIQDGLTSGNYGPKSIPTHSCVVQSLSIEREPQDNSSNYPDDLWRVTEEQRDYYINQFKSLQPDLNSFISGSVAKNFFTKSKLPIPELSHIWELSDVDCDGALTLAEFCVAFHLVVARKNGYLLPDSLPETLLPDYLHAASLKPKGEYILNSYSESLSVNQSTHLSRTELPIDEKPVKAALIQDTVTDDQGLKVSDSTQAAPKEFKVSQHLPFKTVAQESNTLKTRPRSRSYSSTSVEDPMNKGGEPPAPPPRPQKSHSRASSLDLNNIFQQNTHVRSGWIPPPPALPPRPFVSQVSLPVINTATSTQPKSSYMNFTLQEQIEQDSEINSHLPVNMSPLQESSPAKKDLVLTQLPSKPIHRKFQPESHLLENQEVSPTKSATASSLPTIKPHHAFPKQSLKQKKAIQTAIRENKEANAVLVRLNSELQQQLKEVHKERIALETQLEQHRPVTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X949</accession>
    <name>A0A6I9X949_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106541257</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013912114.1">
        <property type="nucleotide sequence ID" value="XM_014056639.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541257"/>
    <dbReference type="KEGG" id="tsr:106541257"/>
    <dbReference type="OrthoDB" id="774951at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20870">
        <property type="entry name" value="C1_A_C-Raf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17135">
        <property type="entry name" value="RBD_CRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000015">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000004">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003116">
        <property type="entry name" value="RBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02196">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00455">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50898">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RBD" evidence="6">
        <location>
            <begin position="76"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="5">
        <location>
            <begin position="158"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="302"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912114.1"/>
        </source>
    </evidence>
    <sequence length="449" mass="50374" checksum="3A3D7A8AC014D05D" modified="2020-10-07" version="1">MGYQCMITELHTVWLLKAASMDHIHGAWKTLPDGIGLKDSVFNGSSCISPTIVEQFGYQRRASDDGKLLDSSKTSNTIRVFLPNKQRTVVNVRNGMTLHDCLMKALKVRGLQPECCAVFQLPTEHKGKKARLDWSTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAYCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPHSNIGDSGISAPPLMTRRIRESVSRMPVNSQHRYSTPHVFTFSGTNPSPECSLSQRQRSTSTPNVHMVSTTMPVDNRIIEDAIRSHSESASPPAMSGSPNNTSPTGWSQPKTPVPAQRERAAXHFAYFTDHWDGSSSDKWVQNTIRHGLSLEFLSSPPKFFRICPVSNNPARRLLMQEAIQHLLDIRDIQPVPLDQQGQGHYSILFVIPKASGDWRAILDLKHLNSCSAVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBZ8</accession>
    <name>A0A6I9XBZ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="4">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106538661</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013908703.1">
        <property type="nucleotide sequence ID" value="XM_014053228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBZ8"/>
    <dbReference type="GeneID" id="106538661"/>
    <dbReference type="KEGG" id="tsr:106538661"/>
    <dbReference type="OrthoDB" id="3200163at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00312">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000011">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027164096" description="Carboxylic ester hydrolase" evidence="4">
        <location>
            <begin position="18"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="5">
        <location>
            <begin position="29"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013908703.1"/>
        </source>
    </evidence>
    <sequence length="561" mass="62656" checksum="0C1C98389BBADCF3" modified="2020-10-07" version="1">MKWWLFGIVIESLLVAGIPTEGERQEHLEVITKYGHLRGKLADVDGMDEPVKSFLGVPFAKPPTGSLRFSPPQPPEPWSHMRDATSHPPMCLQDLGWVDVLKEIVNITLPNLMVSEDCLYLNIFTPDMEAKLPVMVWLPGGGLVNGGASFYDGSALSAYGNVVVVAVQYRLGILGFLSTGSQEASGNWGLLDQVAALQWVQENIEAFGGDPSCVTIFGESAGGFSVGAQILSPLSKGLFHRAISESGTVQLPGFFTQHPEILAQKVASAADCETTSPAMLLCLRNKTEEELNSLISRLPPELHLIPAVVDGEFILKAPEELLASKELHAVPYLIGVNNNEYGWFLYGSSLVQNSSYIREGMDRERIAAALQRMASIWNYPPEIMQMILDKYLEATEDPVELRSRFQKIMEDAIFVIPSLQAARYHRDSAAPVYFYEFQHRPTAFKDTKPDFVKADHGDELGFVFGGPFLRSKSFTGEATEDEKQLSRTIMKYWSNFARNGDPNGEGLVEWPSYGLQEEYLELNLEQRKSEKLRNSYVDFWLKALPEKLKTIMEEEKTHPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDE6</accession>
    <name>A0A6I9XDE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">5-azacytidine-induced protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">AZI2</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912235.1">
        <property type="nucleotide sequence ID" value="XM_014056760.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912236.1">
        <property type="nucleotide sequence ID" value="XM_014056761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDE6"/>
    <dbReference type="GeneID" id="106541350"/>
    <dbReference type="KEGG" id="tsr:106541350"/>
    <dbReference type="CTD" id="64343"/>
    <dbReference type="OrthoDB" id="8744179at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024581">
        <property type="entry name" value="TBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051891">
        <property type="entry name" value="TBK1-IKBKE_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14432:SF6">
        <property type="entry name" value="5-AZACYTIDINE-INDUCED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14432">
        <property type="entry name" value="PROSAPIP2 PROTEIN/5-AZACYTIDINE INDUCED GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12845">
        <property type="entry name" value="TBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Tbk1/Ikki binding" evidence="9">
        <location>
            <begin position="215"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="125"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013912236.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="43754" checksum="BAAF13B003234EDE" modified="2020-10-07" version="1">MEELAEDDIDILNHEKADTHHRQDGQFPTPVFSGEESVASHFALVTAYEDIKKRLKETERENSFLKKRVRLLEEKLLESHLEEATSSVGREQVNKAYQAYRESCIERDNLKSQLDKTMKENMEYVKILNEQLQSKEVELLQLRTEVETQQVMKSFNSNPSNWEIDKLNSELKMHNLEVDLEKLREECNHLRKELQKSVESQESLINGELIQQQNIHSERIQQVYRELKREMSNLHLVTEVQVEMLKKLKLIPSTTKKVPSYTSVQCIEDLERDATNLHLGASGAICKNRLLQTNDDVFGNLASSSPCTNEKFSSSCESPFLEHNSYGKNSLEDNSWVFPSPPKPSETVFWEMKNRVTHLNFPGNNLDQYNQNCLHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEK2</accession>
    <name>A0A6I9XEK2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable cationic amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SLC7A14</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912292.1">
        <property type="nucleotide sequence ID" value="XM_014056817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEK2"/>
    <dbReference type="GeneID" id="106541389"/>
    <dbReference type="KEGG" id="tsr:106541389"/>
    <dbReference type="CTD" id="57709"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000010">
        <property type="entry name" value="probable cationic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF17">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="585"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="11">
        <location>
            <begin position="557"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="643"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="646"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013912292.1"/>
        </source>
    </evidence>
    <sequence length="700" mass="76642" checksum="5B0522F9721136D4" modified="2020-10-07" version="1">MYVVSGLVAKEMAGPGVIVSFIIAAVASILSGVCYAEFGVRVPKTTGSAYTYSYVTVGEFVAFFIGWNLILEYLIGTAAGASALSSMFDSLANHTISHWMIDKVGTLNGLGKGEESYPDLLALVIAAIVTIIVALGVKNSVGLNNVLNVINLVVWIFIMIAGFTYVNGDYWAEGQFLPFGWSGVLQGAATCFYAFIGFDIIATTGEEAKSPNTSIPYAITASLVICLVAYVSVSIILTLLVPYNDIDTESPLMEMFVAHGFYTAKFIVAIGSVAGLTVSLLGSLFPMPRVIYAMAGDGLLFRFLAHVSSYTETPVVACIVSGFLAALLSLLVSLRDLIEMMSIGTLLAYTLVSVCVLLLRYQPESDIDGFVKFLSEEHTKKKEGILADCEKEVCSPVSEGEDFAGPPTNTCGAKNLPSLGDNEMLIGKSDKSTYNVNHPNYGTVDMTSGIEADESENIYLIKLKKLVGPRYYTMRIHLGLPGKMDRPTAATGQTVTTCVLLLFVLMFVFCSFIIFGVDYLYDQSWWAILLVVLMVLLIVMLVFVILQQPENPKKLPYMAPCLPFVPAFAMLVNIYLMLKLSKITWIRFAVWCFVGLLIYFGYGIWNSTLEITAREEALHQSTYQRYDVDVDPFSIEEGLDSAGNEKYQDWDPADDKGFSYQQMAEGKESSRTSSKSKNKAKHKPNSDALISNDELDYSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEK7</accession>
    <name>A0A6I9XEK7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Synaptotagmin-6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SYT6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909538.1">
        <property type="nucleotide sequence ID" value="XM_014054063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEK7"/>
    <dbReference type="GeneID" id="106539297"/>
    <dbReference type="KEGG" id="tsr:106539297"/>
    <dbReference type="CTD" id="148281"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08403">
        <property type="entry name" value="C2B_Synaptotagmin-3-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000005">
        <property type="entry name" value="Synaptotagmin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000011">
        <property type="entry name" value="Synaptotagmin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF45">
        <property type="entry name" value="SYNAPTOTAGMIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="219"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="351"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909538.1"/>
        </source>
    </evidence>
    <sequence length="551" mass="62648" checksum="15FB3D3AB9C41C56" modified="2020-10-07" version="1">MSAAKQETQCQTAARIVTDLCRQKSLPQLDLETCKEFLLFPDLNGPEPELSISLLAVIVIVCGLALVAVFLFLFWKLCWVPWRNKSISTNTPTTQNLAEHDKDNMADKLKDTGTLGFLEAAVKISHTSPDIPAEVQMSMKDHLMRRTRMQRQTTEPASSTRHISFKRHLPRQMHVSSVDYGTDLPLAAEQPTCIGRIKPELYKQKSVDSEDPKKEAEKTCGKINFSLKYDYENETLIVRILKAFDLPAKDLCGSSDPYVKIYLLPDRKRKFQTRVHRKTLNPTFDECFQFPVPYEELTNRKLHLSVFDFDRFSRHDMIGEVILENLFEASDLSRETSIWKDIQYATTESVDLGEIMFSLCYLPTAGRLTLTVIKCRNLKAMDITGYSDPYVKVSLLCDGRRLKKKKTTIKRNTLNPTYNEAIIFDIPPENMDQVSLLISVMDYDRVGHNEIIGVCRVANNAEGLGRDHWNEMLAYPRKPIAHWHPLVEVKKSFKEQCPGLNAPKAVMPVAEPSNPLSTMMLPSSKYSKWHGRAASFDSQSSCPSPKPPTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XF10</accession>
    <name>A0A6I9XF10_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Geranylgeranyl transferase type-1 subunit beta</fullName>
            <ecNumber evidence="4">2.5.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Geranylgeranyl transferase type I subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PGGT1B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.59" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013912462.1">
        <property type="nucleotide sequence ID" value="XM_014056987.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541511"/>
    <dbReference type="KEGG" id="tsr:106541511"/>
    <dbReference type="CTD" id="5229"/>
    <dbReference type="OrthoDB" id="24893at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02895">
        <property type="entry name" value="GGTase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041960">
        <property type="entry name" value="GGTase_I_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="13">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="13">
        <location>
            <begin position="89"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013912462.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="33127" checksum="BD1404A9B8717428" modified="2020-10-07" version="1">MASTVLVEDEGGFGDGERLDFLKERHVRFFQRSLQILPERYSSLETSRLTVAFFALSGLDMLDSLDVVNKEDIIEWIYSLQVLPTEDISSFCAVLEGSENDMRFIYCAACICYMLNSWSGMNTKKAIDYIRRSMSYDNGLAQGAELESHGGSTFCGIASLCLMGKLEEVFSEKELKRIRRWCIMRQQNGYQGRPNKPVDTCYSFWVGATLKLLKIFQYTNYEKNRNYILSTQDRLVGGFAKWPDSHPDALHAYFGICGLSLMGESGIRKVHPALNVSTXMQVSRIVPVHCLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG32</accession>
    <name>A0A6I9XG32_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Exostosin-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EXTL1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910008.1">
        <property type="nucleotide sequence ID" value="XM_014054533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG32"/>
    <dbReference type="GeneID" id="106539688"/>
    <dbReference type="KEGG" id="tsr:106539688"/>
    <dbReference type="CTD" id="2134"/>
    <dbReference type="OrthoDB" id="5954868at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261">
        <property type="entry name" value="ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261:SF3">
        <property type="entry name" value="EXOSTOSIN GLYCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="14">
        <location>
            <begin position="103"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="15">
        <location>
            <begin position="472"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="664"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013910008.1"/>
        </source>
    </evidence>
    <sequence length="736" mass="85426" checksum="A03FB7EF8998618B" modified="2020-10-07" version="1">MQAKKKYLFVTLLATWLLLVLFGGDQIRRFPFFSNKKAEVLKTWPRWADEVLLKNFAGSEELQSTERGHSDESPQSRQQLKLSLYKNSRCRMETCFDFSKCRQHGFKVFVYPLEGGDPVSESYLKIISSIEESRYYTSNPEEACLFVLNIDTLDRDHLSIQYVHNINEKIQGFQLWNDGQNHLIFNLYSGTWPNYTEDLGFDIGQAILAKASFYVENFRPGFDISIPLFSKEHPQKGGERGWLYKTSLPPKKKYLLVFKGKRYLTGIGSDTRNALRHIHNGKDIISLATCKHGKDWEKHKDKRCDKDNIEYEKFDYQELLHNSTFCIIPRGRRLGSFRFLEALQAACIPVLLSNGWELPFSEVIDWSKSSIVGDERLLLQIPSTVRCLNSDKILAFQQQTQFLWETYFSSVEKIVQTTLEIIKDRIFHQESRPKFFWNTLPGGLVALPEYSTHLNDFPFYYPQQGSSPSEKFTALIWAVSPIVSLSQPILKLTQAVSRSQYCAKILVLWSCEKPPPQKWPPTTVPLTVILSSNKVSERFLPYSALGTDAVLSLDEYTSLSTSEVDFAFVVWRKFPDRIVGFPMRSHFWDTGKNQWIYTSKWTNEFSMVLTAAAFYHRYYHSLFSNYLPAKLRNFVDQIANCEDILMNFLVSAVTKLPPIKVTQKKHLRESPPQQLLKSAPPSNSSLHFAQRQECVNHFAAWFGYMPLVHSQFRLDPVLFKDQVSVLRKKYRQMERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH64</accession>
    <name>A0A6I9XH64_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable ATP-dependent RNA helicase DHX40</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106542087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913207.1">
        <property type="nucleotide sequence ID" value="XM_014057732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH64"/>
    <dbReference type="GeneID" id="106542087"/>
    <dbReference type="KEGG" id="tsr:106542087"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF271">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX40-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="125"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913207.1"/>
        </source>
    </evidence>
    <sequence length="218" mass="24337" checksum="8A69834498E35BF6" modified="2020-10-07" version="1">MSQETAIKYLTDGCLLRQILADPHLAQYSVVILDEAHERSLCTDILFGLLKQLFHGEKEIQRKEHLKVVVMSATLDVEKFSAFFGNCSVVEIPGRKYLVEEIFCNALGPRDANNSAFITETVRVTLDVHLNGSAGDILVFLTGQSEIERACELLFQKAEMIDYRFEVRDRSVDGLLILPLYGCMPTDQQRQIFVSPPPGIRKCVVSTNIAATSLTIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHG8</accession>
    <name>A0A6I9XHG8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Metabotropic glutamate receptor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">GRM1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915159.1">
        <property type="nucleotide sequence ID" value="XM_014059684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHG8"/>
    <dbReference type="GeneID" id="106543622"/>
    <dbReference type="KEGG" id="tsr:106543622"/>
    <dbReference type="CTD" id="2911"/>
    <dbReference type="OrthoDB" id="425344at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008066">
        <property type="term" value="F:glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007206">
        <property type="term" value="P:phospholipase C-activating G protein-coupled glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15449">
        <property type="entry name" value="7tmC_mGluR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06374">
        <property type="entry name" value="PBP1_mGluR_groupI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000219">
        <property type="entry name" value="Glutamate metabotropic receptor 5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000001">
        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000337">
        <property type="entry name" value="Metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001256">
        <property type="entry name" value="GPCR_3_mGluR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000162">
        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019588">
        <property type="entry name" value="Metabotropic_Glu_rcpt_Homer-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10606">
        <property type="entry name" value="GluR_Homer-bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01051">
        <property type="entry name" value="MTABOTROPC1R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01229">
        <property type="entry name" value="GluR_Homer-bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="513"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="581"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="703"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="731"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="21">
        <location>
            <begin position="511"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="797"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="988"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="803"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="993"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1033"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1062"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013915159.1"/>
        </source>
    </evidence>
    <sequence length="1105" mass="123749" checksum="EFEB5875D230DBB1" modified="2020-10-07" version="1">MFHTLDKINADPFLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDALGRCLPDPLHHHQIRAKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDILQARAMLDIVKRYNWTYVSAVHTEGNYGESGMEAFKELAAQEGLCIAHSDKIYSNAGEKSFEKLLQKLRERLPKARVVVCFCEGMTVRGILMAMRRMGVFRELLLIGSDGWADRDEVIEGYEAEASGGITIKLQSPDVVSFDDYFLKIKLETNKRNPWFPEFWQHRFQCRLPGHPLENPNFQKNCTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHTLCPGHVGLCDAMKPIDGSKLLESLLNSSFSGVSGEEVRIDENGDAPGRYDIMNLQQIESNRYDYVHIGTWHEGVLNIDDNRIQMNKTEMVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEFVQDEFTCKACELGWWPNDELTGCQPIPVKYLEWRDIESVVAVAFSCLGILITMFVTLIFALYRDTPVVKSSSRELCYIILAGIFLGYICPFTLIAKPTVTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPHFMSAWAQVVIASILISVQLTLVITLIIMEPPFPVLSYPSIKEVFLICNTSNLGVVAPVGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVGDGKTPCRSNTFLSIFRRKKPGTGNPNSNGKSVSWSEPGGREIPKGQHMWHRLSVHVKNKELRCNQTAVIKPLTKSYPGPSKSLTFSDISSKTLYNVVEEEASESVHFSQMSPSMVVHRRVMVMPPLQAAAEDIQSPKVLPPPPPPPPPPRFIMEQLQGVVNKFATGIPDFNVVMPVSGAGNGLRPIYNPPAMQPQILPLQMGTFSKVPVSLPSEEEEEEEEEEEEEEEEEEEEEENEKFKLIQDYVYERPGNLEEEEEEEEEQATSKLTLEDSPALTPPSPFRDSVASSSSVPSSPVSESVLCIPPNATYTTVVLRDYKQSSSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI10</accession>
    <name>A0A6I9XI10_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cytochrome-b5 reductase</fullName>
            <ecNumber evidence="2">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106540106</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013910588.1">
        <property type="nucleotide sequence ID" value="XM_014055113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI10"/>
    <dbReference type="GeneID" id="106540106"/>
    <dbReference type="KEGG" id="tsr:106540106"/>
    <dbReference type="OrthoDB" id="432299at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000001">
        <property type="entry name" value="Cytochrome b5 reductase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000019">
        <property type="entry name" value="cytochrome b5 reductase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="9">
        <location>
            <begin position="20"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="10">
        <location>
            <begin position="133"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013910588.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="59880" checksum="151FDDA9C849C7E8" modified="2020-10-07" version="1">MDWIRLTKSGQDLTGLKGRLLEVTEEELMKHNKREDCWICIRGLVYNVTPYMEYHPGGEDELMKAAGIDGTDLFDQVHRWVNYESMLKECLVGRIVLKPATSVKEICLPVTEENRTLNGILPGKKVLDPYSKEIIPSYDWFQTEDSVTVVIYTKQKDVDSESVIADVQERTLRGEIIVKDFSYLIHVELSYAVYEDIIVQISEKVGKVEFVLKKKENLSWKKLGQPLKRHNSFVKCTDRGNTFYCSAQSSFLLWVVLLHGLSLGTEIVKPYTPILSCLVSPFKISSSNDKAQIFFMIKIYPGGLFTPVLDTLQIGDYISLSNPQGIFKTSQLEDVEDLFLLAGGTGFTPMVKLLDYAFSNSATLRYFSVLRPIINVSFSFGSFKAFSFYCFDNICFYVIRVWNAESHQYEENCVLSAEITIKKRSSAGSGKSALGQAKKMRGLPCSRFIPTTSNLRFDIQFVLSEPKKNWAGKQGKISLSLLYEFVKKSKPESKILICICGPFPFMEQGIQFLQTLGHSGNEVHCFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI42</accession>
    <name>A0A6I9XI42_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Synaptic vesicle membrane protein VAT-1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">VAT1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913731.1">
        <property type="nucleotide sequence ID" value="XM_014058256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI42"/>
    <dbReference type="GeneID" id="106542503"/>
    <dbReference type="KEGG" id="tsr:106542503"/>
    <dbReference type="CTD" id="10493"/>
    <dbReference type="OrthoDB" id="203908at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010637">
        <property type="term" value="P:negative regulation of mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08275">
        <property type="entry name" value="MDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052100">
        <property type="entry name" value="SV-ATPase_mito-regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44054:SF1">
        <property type="entry name" value="SYNAPTIC VESICLE MEMBRANE PROTEIN VAT-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44054">
        <property type="entry name" value="SYNAPTIC VESICLE MEMBRANE PROTEIN VAT-1 HOMOLOG-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="75"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913731.1"/>
        </source>
    </evidence>
    <sequence length="420" mass="44703" checksum="FB654B8C9006884F" modified="2020-10-07" version="1">MSGEETVGEDAGAPQTSTVAPPAEQQQNEGAVVEATATGGEGKAAEGTSEEAPAVSTSTTASGRPYRALVLTGFGGYEKVKVQIRQLPQAEPSPGAGQVCVQVRACGLNFADLLARQGLYERLPSPPVSPGMEAAGIVLAVGEGVSSPKVGDKVMIMARSGLWQEIVTVQANQSFPMPEGMSFEEAAAFLVNYITAYMVLFDFGNLRPGQSVLVHMAAGGVGTAAVQLCKTMENITIFGTASASKHEALKENGVTHPIDYRAADYVAEVRKICPKGVDVVLDPLGGSDTTKGFHLLKPMGKLVSYGVANLLTGQRKNLMAMAKTWWNQFSINALQLIHLNKAVCGYHLGYLEEEVEVLAAVVTKLTALYNQGKIKPKVDSVWPFEQVVDAMKQMQEKKNVGKVVLVPEAPPKDENKKTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM24</accession>
    <name>A0A6I9XM24_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable fibrosin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106545006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916919.1">
        <property type="nucleotide sequence ID" value="XM_014061444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM24"/>
    <dbReference type="GeneID" id="106545006"/>
    <dbReference type="KEGG" id="tsr:106545006"/>
    <dbReference type="OrthoDB" id="10060000at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023246">
        <property type="entry name" value="AUTS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429:SF24">
        <property type="entry name" value="FIBROSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429">
        <property type="entry name" value="FIBROSIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15336">
        <property type="entry name" value="Auts2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02044">
        <property type="entry name" value="FIBROSIN1LPF"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916919.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="37537" checksum="1BA409E62482F200" modified="2020-10-07" version="1">MHVHVAYMILRHQEKIKLMQDDPHKLDFQNDLTGLPETGAFGSLPHSHELARPATLFTAAGVVQPGNASSFAPAAATHGSFLNPSPHIDPFGRPPSFASLGALSNGAFGGLGNPSFNSSAVFGQKDTPGAPAFPSPHEAWNRLHRTPPSFPTAPAWPKGGGSNGTERGSSQLDKESEKPEGPVIKDEKDRDPGFFSRHSLRSSPATPTSKNPSLIHEEPPSRPHLQAEREHRYGSPASSGHASRSPYPELPLKKEVKIKEEPDMPGFEGALRTGASYHSTLHVSHPLGTLAVFERPQAGTGGSVSPYLVAAPPSAASYAALEAWREPYHHRGLELHPLQRLPRYLETPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XN48</accession>
    <name>A0A6I9XN48_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integrin-linked protein kinase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106545373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917374.1">
        <property type="nucleotide sequence ID" value="XM_014061899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XN48"/>
    <dbReference type="GeneID" id="106545373"/>
    <dbReference type="KEGG" id="tsr:106545373"/>
    <dbReference type="OrthoDB" id="9944853at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000245">
        <property type="entry name" value="Integrin-linked protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF57">
        <property type="entry name" value="INTEGRIN-LINKED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="108"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013917374.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="23344" checksum="C6D2806600ED97B1" modified="2020-10-07" version="1">MFIPPGPKEKTGFPKTPRALSIHLSASPCQFPWVNLGRGGGRNGRKRQWGSSSPSSLTAPSALPERAEKSGQSLTRLPYKDTFWKGTTRTRPRNGTLNKLAGIDFRQLSLGHKLNENQSGELWKGRWQGNDIVIKVLKIRDWTTRKSRDFNEEYPKLRIFSHPNVLPVLGACQSPPAPHPIIITHWMPYGSLYNVLHEGTSESCHQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNU9</accession>
    <name>A0A6I9XNU9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box/LRR-repeat protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FBXL6</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917644.1">
        <property type="nucleotide sequence ID" value="XM_014062169.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106545564"/>
    <dbReference type="KEGG" id="tsr:106545564"/>
    <dbReference type="CTD" id="26233"/>
    <dbReference type="OrthoDB" id="3134645at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013917644.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="28692" checksum="3E01673E69C4A1AF" modified="2020-10-07" version="1">MRTQXCCPDLRLLEVNAEIEQSTQEFQLPIEQLQAACPQLQVLRLLNLTYYPKQPPSSSPPSLGFPQLEELCLATTAFSYVDNHMLCRILSTSSRLRVLDLRGCVRVTPEGLEQLPCPDLEQLYLGLHYSDSSLSRPLEGSPLLTWKWRHSLRELDLTSQSFSEEDLEQAMAAFTQGGGEAGAPQLRFLSLTGTKVALRTVRSLIASCPALTYLNLSSCRQLPRGSKKAYRGPEEIRQCLSQLLSRSEEDPGTPAGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNW3</accession>
    <name>A0A6I9XNW3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106541483</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912428.1">
        <property type="nucleotide sequence ID" value="XM_014056953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNW3"/>
    <dbReference type="GeneID" id="106541483"/>
    <dbReference type="KEGG" id="tsr:106541483"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13954">
        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF357">
        <property type="entry name" value="OLFACTORY RECEPTOR FAMILY 6 SUBFAMILY Q MEMBER 1 (GENE_PSEUDOGENE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="43"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013912428.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35667" checksum="6CB2615E7E65542C" modified="2020-10-07" version="1">MQSPHENWTLVTEFVLASFTGIQEAWLPLFGFFLIMYLLTLMENGILVLVVATGKRLHTPMYFFLIHLSFLDIWYTSLTVPKMLAGFGYPAGQRISYSACLTQLYLFTFLGSTECFLLAAMAYDRYVAICIPLRYQEIVDQNTCLILAIGSWLTGFLTPVLPVYFMSQLTFCGPNVIDHFFCDASPLLILSCTDISLKESVDFLVSLFVLLISSSVIITSYVHIIITVLRINSSVGRQRAFSTCTAHLMVVAVFYGTLFFMYVRIKVTSSINLNKVVSVFYAIVTPVLNPLIYSLRNTEVKAALCRIIFKKKPVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP18</accession>
    <name>A0A6I9XP18_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase TRIM36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TRIM36</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912463.1">
        <property type="nucleotide sequence ID" value="XM_014056988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP18"/>
    <dbReference type="GeneID" id="106541512"/>
    <dbReference type="KEGG" id="tsr:106541512"/>
    <dbReference type="CTD" id="55521"/>
    <dbReference type="OrthoDB" id="10040278at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007340">
        <property type="term" value="P:acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19848">
        <property type="entry name" value="Bbox1_TRIM36_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19778">
        <property type="entry name" value="Bbox2_TRIM36_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16756">
        <property type="entry name" value="RING-HC_TRIM36_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.40:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040859">
        <property type="entry name" value="Midline-1_COS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047066">
        <property type="entry name" value="TRIM36_Bbox1_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047065">
        <property type="entry name" value="TRIM36_Bbox2_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027726">
        <property type="entry name" value="Trim36_HC-RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF18">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18568">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="COS" evidence="12">
        <location>
            <begin position="279"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="351"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912463.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="63564" checksum="2A94AE639924304C" modified="2020-10-07" version="1">MEGDRLEPVVTIKNIERELICPACKELFTHPLILPCQHSICHKCVKEILYLTFEDSFNDAASETSNHSSPRSRMNSPSLDRIDRVNRSGWKRNSLTPRTTTFPCPGCRHDVDLGERGISGLFRNVTLETIVERYRQAARAATAIMCDLCKPPAQESTKSCMDCGASYCNECFKIHHPWGTLKAQHEYVGPTTNFRPKVSVNMLSCFNLKKKQCNGERAKEEASNNFDKLFHVLEEKKNTAIRAIETSKHLRMDKLHSQIEEYQGLLENNGLVGYAQEVLKETDPSCFVQTAKQLHDRIQKATESLKSFRPAAQASFEDFVLDIEKQQEALENMCFHSNENVNIQPYEYYRISILMCPEHEMEKVNMYCEICRRPVCHLCKLGGTHANHRVTTMSSAYKTLKEKLSQDIHYLISKESHVKNQITELGLLVKEAEYSFFRYEQDSGHDSGSEDIFAESLQPFTLITIGMKKMFIPKASSASAAPKDCVNRVLPMPSNIGICLDCDKGSIGFYDADRMKCIYERPVECSGKMYPAFALMGSSGIHLEETIAAKYLQYQDEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP57</accession>
    <name>A0A6I9XP57_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 13C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106541543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912503.1">
        <property type="nucleotide sequence ID" value="XM_014057028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP57"/>
    <dbReference type="GeneID" id="106541543"/>
    <dbReference type="KEGG" id="tsr:106541543"/>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF125">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912503.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="28425" checksum="A3062AA8220C4A38" modified="2020-10-07" version="1">MTLAELSLQTTNENWTPCILNDAAKIIYKLLSLDSLSIYWNVESEMYYRSSREQILERLKKGVPSMNQELPDYQYIFKPVSASAKLYLNPHAEEELETPKVDCAMEVQSIAVELTKPQYLSMIDLLESIDYMVRNAPYRKYRPDVPLHRNTKQWWKYAGNCILDLHIKRYMQMWSWDHIKSHRQLLKSYKNVYKVKLTQAKLSEENQKQIQDLEKALDVFNIVLARQQAQTEVVRSGQKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ62</accession>
    <name>A0A6I9XQ62_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="36">Potassium channel subfamily K member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="42" type="primary">KCNK1</name>
    </gene>
    <organism evidence="41 42">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="42" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>L-glutamate(out) = L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:66336"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>Li(+)(in) = Li(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78551"/>
            <dbReference type="ChEBI" id="CHEBI:49713"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NH4(+)(in) = NH4(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28747"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="35">Homodimer; disulfide-linked. Heterodimer with KCNK2; disulfide-linked. In astrocytes, forms mostly heterodimeric potassium channels with KCNK2, with only a minor proportion of functional channels containing homodimeric KCNK1. Interacts with KCNK3 and KCNK9, forming functional heterodimeric channels. Interacts with GNG4. Identified in a complex with PSD and ARF6; interacts only with PSD that is bound to ARF6. Interacts with UBE2I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 38">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912843.1">
        <property type="nucleotide sequence ID" value="XM_014057368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ62"/>
    <dbReference type="GlyCosmos" id="A0A6I9XQ62">
        <property type="glycosylation" value="1 site, No reported glycans"/>
    </dbReference>
    <dbReference type="GeneID" id="106541827"/>
    <dbReference type="KEGG" id="tsr:106541827"/>
    <dbReference type="CTD" id="3775"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000076">
        <property type="entry name" value="Potassium channel subfamily K member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005408">
        <property type="entry name" value="2pore_dom_K_chnl_TWIK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001779">
        <property type="entry name" value="2pore_dom_K_chnl_TWIK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF59">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038061">
        <property type="entry name" value="K_channel_subfamily_K_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01096">
        <property type="entry name" value="TWIK1CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01586">
        <property type="entry name" value="TWIKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="24" id="KW-0966">Cell projection</keyword>
    <keyword evidence="26" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0967">Endosome</keyword>
    <keyword evidence="23" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="25 38 42" id="KW-0407">Ion channel</keyword>
    <keyword evidence="20 36 38" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="21 36 39" id="KW-0472">Membrane</keyword>
    <keyword evidence="17 36" id="KW-0630">Potassium</keyword>
    <keyword evidence="15 36" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="12 36" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0770">Synapse</keyword>
    <keyword evidence="13 38 39" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 39" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 36 38" id="KW-0813">Transport</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="39">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="39">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="39">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="39">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="39">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="40">
        <location>
            <begin position="93"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="40">
        <location>
            <begin position="190"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="37">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00036683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00044635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00044657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00044691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00046361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="PIRNR" id="PIRNR038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="PIRSR" id="PIRSR038061-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="RefSeq" id="XP_013912843.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="37949" checksum="21F33A9FDEFE06DB" modified="2020-10-07" version="1">MLQSLAGSSCVERHRSAWCFGLLVLGYLLYLVFGAVVFSSVELPYEDLLRQELRKLKQRFVEEHECLSEEQLESFLARVLQASNYGVSALSNASGSWNWDFTSALFFASTVLSTTGYGHTVPLSDGGKAFCIIYSVIGIPFTLLFLTAVVQRIIVYVTRRPVLYFHIHWGFSKQIVAIIHAVILGFVTVSCFFFIPAIIFSVLEDDWNFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKIFYVKKNKEEDQVHITEHDQLSFSSISDQAASMKEDQKIEEPFVTPQHAVNCDDFINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR45</accession>
    <name>A0A6I9XR45_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106542057</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the InsP3 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913148.1">
        <property type="nucleotide sequence ID" value="XM_014057673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR45"/>
    <dbReference type="GeneID" id="106542057"/>
    <dbReference type="KEGG" id="tsr:106542057"/>
    <dbReference type="OrthoDB" id="76898at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.30:FF:000001">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor, type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.30">
        <property type="entry name" value="IP3 receptor type 1 binding core, RIH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF3">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00779">
        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="2" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RIH" evidence="4">
        <location>
            <begin position="60"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913148.1"/>
        </source>
    </evidence>
    <sequence length="756" mass="87142" checksum="FBA6646C9EB8DEED" modified="2020-10-07" version="1">MLKIGTCQTKEDKEAFAIVSVPLSEVRDLDFANDANKVLASMVKNLENGSVTQNERRFVTKLLEDLIFFVADVHNNGQEVLDVVVIRANRERQKLMREQNILEQIFGILKAPFKEKDGEGAMLRLEDLGDQRYAPYKYMLRLCYRILRHSQQDYRKNQEYIATNFCIMQSQIGYDILAEDTITALLHNNRKLLEKHITAKEIETFVSLLRRNREPRFLDYLSDLCVSNTAAIPVTQELICKYMLSPGNADILIQTKLVSMQMDNPLNCSSLSDDMEEEEVWLYWIDSNKEPHGKAIRHLAQEAKEGTKTDLEVLTYYRYQLNLFARMCLDRQYLAINQISTQLSVDLILRCMSDESLPYELRASFCRLMLHMHVDRDPQETGVPVKYARLWTEIPTKITIHEYDSFTDSSRNDMKRKFAFTMEFVEDYLKEVVNQPFPFGDKEKNKLTFEVVHLARNLIYFGFYSFSELLRLTRTLLAILDIVQAPMSSYLERLSKFQDGGNNVMRTIHGVGEMMTQMVLSRGSIFPASSPDILPSVHRSKQANLMESEDVTVMDTKLKIIEILQFILSVRLDYRISYMLSIYKKEFGESSENVENSTLSPSDSPTLTSVAVPDIDEIAAEAETMFAGRKEKNAVQLDDEGGRTFLRVLIHLIMHDYPPLLSGALQLLFKHFSQRSEVLQAFKQVQLLVSNQDVDSYKQIKTDLDQLRLTVEKSELWVQKTSNYENGVLGENQTKGSDEPNEVSIRSFTSFLLSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSE3</accession>
    <name>A0A6I9XSE3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tubulin alpha chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106546598</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918979.1">
        <property type="nucleotide sequence ID" value="XM_014063504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSE3"/>
    <dbReference type="GeneID" id="106546598"/>
    <dbReference type="KEGG" id="tsr:106546598"/>
    <dbReference type="OrthoDB" id="6073114at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000014">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000079">
        <property type="entry name" value="Tubulin, alpha 4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="10">
        <location>
            <begin position="97"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013918979.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="29404" checksum="81E1ED815B2E78EA" modified="2020-10-07" version="1">MYLQANPWPRPNSSESRCKNAPPSLRFEFSESSATKQQLCSHLKQELTAVRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIEYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSN4</accession>
    <name>A0A6I9XSN4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenosine deaminase</fullName>
            <ecNumber evidence="5">3.5.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ADA</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013916746.1">
        <property type="nucleotide sequence ID" value="XM_014061271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSN4"/>
    <dbReference type="GeneID" id="106544871"/>
    <dbReference type="KEGG" id="tsr:106544871"/>
    <dbReference type="CTD" id="100"/>
    <dbReference type="OrthoDB" id="272271at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060205">
        <property type="term" value="C:cytoplasmic vesicle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060169">
        <property type="term" value="P:negative regulation of adenosine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009168">
        <property type="term" value="P:purine ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042110">
        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01320">
        <property type="entry name" value="ADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000057">
        <property type="entry name" value="Adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF43">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="11">
        <location>
            <begin position="119"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916746.1"/>
        </source>
    </evidence>
    <sequence length="464" mass="52208" checksum="7BC422BC0421F801" modified="2020-10-07" version="1">MSNEENTREGSAPRSCGVDSGRGSRDNKQLSNYKEISPWHSANHRRAEREVCSFQGKTSGVLSAGVSASNSQPKKQRLLGVQEKRQVRPRTCCSLTLGLSKTGGSRSMLPPPLTAELARIPVHLDGAIKPETILHFGKKRGIHLPADTTDGLLKQITCDKPGCLTTFLQKFDLYMPAIAGDREAIRKIAYEFVEMKAKEGVIYVEVRYCPHLLANCEVDPIPWNQKKGDVTPDDVVNCVNQGLKDGEKDFNIKVKSILCCMRDKPCWSPEILRLCKKYWNDTVVAIDLAGDESIIADCSGHLEAYKEAESSGIHRTVHAGEVGSSQVIKEAVNILKAERIGHGYHVFEDEQLYKQLLEQNVHFELCPWSSYLTGACKTSFKEHPVKRFKNDGANYSLNSDDPLIFRSTINTDYRITQEYMEFTEQEFEKLNINAAKSCFLEEKEKKELVYKLQKAYGMVKSTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT56</accession>
    <name>A0A6I9XT56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="9">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PDK3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_013916941.1">
        <property type="nucleotide sequence ID" value="XM_014061466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT56"/>
    <dbReference type="GeneID" id="106545023"/>
    <dbReference type="KEGG" id="tsr:106545023"/>
    <dbReference type="CTD" id="5165"/>
    <dbReference type="OrthoDB" id="241648at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.20:FF:000001">
        <property type="entry name" value="[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000007">
        <property type="entry name" value="Mitochondrial pyruvate dehydrogenase kinase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF21">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE ISOZYME 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="212"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916941.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="43571" checksum="DD2A71722C010FD6" modified="2020-10-07" version="1">MKTNSFLSTGRDNACEKTSYMFLRKELPVRLANTMREVNLLPENLLNRPSVKLVQSWYMQSFLELLQYENKSPEDPHVLDNFLDILIKVRNRHNDVVPTMAQGVIEYKEKYGFDPFVSSNIQYFLDRFYTNRISFRMLINQHTLLFGGVTNPAHPKHIGSIDPTCNVADVVEDAFATAKMLCEQYYQVAPDLEIEEFNAKAPGRPIQVVYVPSHLFHMLFELFKNSMRATVELYEGKCKSYPSIKTLVTLGKEDLSIKISDKGGGVPLRKIDRLFNYMYSTAPRPSLEPSRAVPLAGYGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNPSSEPRDASKYKANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTW9</accession>
    <name>A0A6I9XTW9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sterol regulatory element-binding protein cleavage-activating protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106547038</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919539.1">
        <property type="nucleotide sequence ID" value="XM_014064064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTW9"/>
    <dbReference type="GeneID" id="106547038"/>
    <dbReference type="KEGG" id="tsr:106547038"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032936">
        <property type="term" value="C:SREBP-SCAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045540">
        <property type="term" value="P:regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057042">
        <property type="entry name" value="Beta-prop_SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030225">
        <property type="entry name" value="SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378:SF1">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24017">
        <property type="entry name" value="Beta-prop_SCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="SCAP beta-propeller" evidence="10">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919539.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="15798" checksum="58913E40C5D26812" modified="2020-10-07" version="1">MVLASGGQDGAICLWDVLTGSRVSHICAHRGDVTSLTCTTSCVISSGLDDVISIWDRASGIKHYSIQQDMGCGASLGVISDNLLVTGGQGCVSFWDIGYGDLLQTVYLGKSNESQPARQILVLENAAIVCNFGSELNLVYVPSVIEKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XU97</accession>
    <name>A0A6I9XU97_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein 395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZNF395</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914258.1">
        <property type="nucleotide sequence ID" value="XM_014058783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XU97"/>
    <dbReference type="GeneID" id="106542916"/>
    <dbReference type="KEGG" id="tsr:106542916"/>
    <dbReference type="CTD" id="55893"/>
    <dbReference type="OrthoDB" id="5950721at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052253">
        <property type="entry name" value="CR1/CR2-DNA-binding_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031940">
        <property type="entry name" value="DUF4772"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006">
        <property type="entry name" value="PAPILLOMAVIRUS REGULATORY FACTOR PRF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006:SF6">
        <property type="entry name" value="ZINC FINGER PROTEIN 395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15997">
        <property type="entry name" value="DUF4772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01366">
        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DUF4772" evidence="10">
        <location>
            <begin position="7"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="228"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914258.1"/>
        </source>
    </evidence>
    <sequence length="605" mass="66460" checksum="B6AEAE9CE9CB7786" modified="2020-10-07" version="1">MASVLSRRLGKRSLLGARISAPPLLGDGMLMTAQVHPLQPEHTRASSHQSILSYEDGHFKEHVEASGNQNQNWPVLQPGQKVYVFYNGQESAGLVEYHDPVSHEVKVLLPEKGFYVTRKDDEVRLAEVMMSPLPKLEMDLGTPTTMDYSPVPVVMEHCTSAADIAVTSALRSVSRSIDVPKRKPDAAVEMDEMMAAMVLTSLSCSPVVQSPPNNEASISTSQATCDAWKESGDMSDGGSSTTSGHWSGISTPSPPHSEASPKYSNEAFSSPRADDGFETDSDPFLFEEPAPRKRKVRSRSLSFSEQPPPPPVLKSQLIVASPPRAPSGTRKVRGEAKKCRKVYGIEHRDQWCTACRRTSEFVRKCVSISVDKGGLAELMIEMIVSAFATKHWKRLLGSYLIYSISPSFIIPCRDGANSDQRKREEDFYYTEMQVKEEALPESLVVAPNPTAGSSPIVIQQAASQPEPLILDQATPLEPHLPSSALSQSAPSSFWHIQADHAYQALPSIQIPVSPHIFTSISWAAATSTIPTLSPVRSRSLSFSEQPPPPPVLKSQLIVASPPRAPSGTRKVRGEAKKCRKVYGIEHRDQWCTACRWKKACQRFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV69</accession>
    <name>A0A6I9XV69_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Msx2-interacting protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106543155</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914548.1">
        <property type="nucleotide sequence ID" value="XM_014059073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV69"/>
    <dbReference type="GeneID" id="106543155"/>
    <dbReference type="KEGG" id="tsr:106543155"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12348">
        <property type="entry name" value="RRM1_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000150">
        <property type="entry name" value="msx2-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034172">
        <property type="entry name" value="SHARP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="6"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="244"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914548.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="35447" checksum="48B0242D32E4695E" modified="2020-10-07" version="1">MVRETRHLWVGNLPENVREEKIIEHFKRYGRVESVKILPKRGSEGGVAAFVDFVDIKSAQKAHNSVNKMGDRDLRTDYNEPGTIPSAARGLDDTVSIASRSREVSGFRAGGGPTYGPPPSLHAREGRYERRLDGASDNRERAYEPSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYSSRSRSPSRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDLTREVRGRRPERSYQHSRSRSPHSSQSRTQSPQRLASQASRPTRSPSGSGSRSRSSSSDSISSSSSTSSDRYRPPSAGVGHLLLWVPVLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVW4</accession>
    <name>A0A6I9XVW4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106546026</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106546026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918257.1">
        <property type="nucleotide sequence ID" value="XM_014062782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVW4"/>
    <dbReference type="GeneID" id="106546026"/>
    <dbReference type="KEGG" id="tsr:106546026"/>
    <dbReference type="OrthoDB" id="9047372at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013918257.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23485" checksum="8B916307DE9B9BA9" modified="2020-10-07" version="1">MNPSSASEMRPYECNQKHQCFSAMEPGLSLTGDWEAPEEEEEKEEEEDSSQFLNVHMRRPRTLRGSSHTYDSFRRHSWEPGKVLEEDTDFDQRSLSLKELAPDGLDSTEQLDGHSLGRRDPQRAPIIHSNNEMGSLFSQDEEDEDGQRMSQVSLPDSGQTNLESKFHRNTQKKVPRIPQFSDEYKYSMFCDELVPCSSPVSQHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWE3</accession>
    <name>A0A6I9XWE3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable uridine nucleosidase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106543455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914963.1">
        <property type="nucleotide sequence ID" value="XM_014059488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWE3"/>
    <dbReference type="GeneID" id="106543455"/>
    <dbReference type="KEGG" id="tsr:106543455"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02649">
        <property type="entry name" value="nuc_hydro_CeIAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914963.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="27050" checksum="77136D0590637DD0" modified="2020-10-07" version="1">MKKLLLIDADCGVDDAQAIMMALANPSVEVLGITCTYGNNLLENTSRNVLRVLQVCNKLEIPVYPGAAAPLLGGPVIGAQFHGEDGLGDAPDPNAPGTELLQKENAVTAILRIINGRPGQVSLVALGPLTNIALAVKMDPSLPKKLKNLFIMGGNTESRGNIKVCGEFNFATDPEAAYIVLNEYTCPMYIAAWEFTCACSLPWVRAFSTLLSILGISFTEFNGVSCWVDRLIKLGCIGSLYSLLEWRCAASAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX73</accession>
    <name>A0A6I9XX73_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin-protein ligase RNF217</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RING finger protein 217</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">RNF217</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the RBR family. RNF217 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013920814.1">
        <property type="nucleotide sequence ID" value="XM_014065339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XX73"/>
    <dbReference type="GeneID" id="106548036"/>
    <dbReference type="KEGG" id="tsr:106548036"/>
    <dbReference type="CTD" id="154214"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20342">
        <property type="entry name" value="BRcat_RBR_RNF217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20350">
        <property type="entry name" value="Rcat_RBR_RNF217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16622">
        <property type="entry name" value="vRING-HC-C4C4_RBR_RNF217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000008">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000264">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047551">
        <property type="entry name" value="BRcat_RBR_RNF217"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047552">
        <property type="entry name" value="Rcat_RBR_RNF217"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047550">
        <property type="entry name" value="RNF217_RBR_vRING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="413"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="173"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013920814.1"/>
        </source>
    </evidence>
    <sequence length="458" mass="50351" checksum="79A4E404101D677B" modified="2020-10-07" version="1">MGERLSTASSPPEPDGAAPEPRLSGTETQPPLPTVSGRTPRPQQEDDAGPPPTEPSGQEAGDGGGAPLEEGPHSEAPSSNSCRLPLRPVSRAESEQSRDGPPPLPSPPLPPTPGEEGRQEDPPPAVSPPPPPSQPSAVGVGGKGTPSGNVEVDLYLLPESFSGLISGDFGTLLVLSCRVCLEEKPLKPLPCCKKPVCEACLKRYLSSQVQVGQADIPCPITECSEHLDETTILFNLPHDDIIKYKYFLELGRIGSSTKPCPQCKHFTTFKRKGHIPTPTKMENKYKIQCPTCQFVWCFKCHSPWHEGVNCKEFKKGDKLLRHWASEIEHGQRNAQKCPKCKIHIQRTEGCDHMTCSQCNTNFCYRCGERYRQLRFFGDHTSNLSVFGCKYRYLPESPHLRRLVRGSVCGGKLLIAPAILVLGLVVGAVVVLIGLFVFPIYCLCKKKKKRPQTEMPWYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXT2</accession>
    <name>A0A6I9XXT2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106546314</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918631.1">
        <property type="nucleotide sequence ID" value="XM_014063156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXT2"/>
    <dbReference type="GeneID" id="106546314"/>
    <dbReference type="KEGG" id="tsr:106546314"/>
    <dbReference type="OrthoDB" id="2018427at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF50">
        <property type="entry name" value="MYOSIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin tail" evidence="4">
        <location>
            <begin position="373"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin tail" evidence="4">
        <location>
            <begin position="489"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="58"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="338"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="696"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013918631.1"/>
        </source>
    </evidence>
    <sequence length="749" mass="85408" checksum="2D839186612E604E" modified="2020-10-07" version="1">MRVPMEGTSRDFLLPHPTLVLGLSLLVWRTWIINMKNCKTPPNFKKLIASTISHSIASSMKQQHSKDPVLKKAQPPSTLQGAGKKASDPVLPPEERDAPGSPSLASGGSVSGEEPMRGLVMSDEEDTGKQDIPSFAGLFQPSLFKQLLTKTKLGGSDSTPTPESQKDPTKTMFVKKPLDKEEVPVPPLFLEVVTTSRHLLWLKNWQVDNKLKFHLTSTPYTRGNLFGEALEPILVENKEKKKVLPLTTKCQENRPAPYRKGSFCTSESEFQQNKFIPYDLEELGRRSRGMAATISQIMGQSGLTIAHWHLLLSIRIDIQLEDNWEQEKKIRAEVEKARRKAEGDLKMTIENLNEMERTKLDLEEVVKPLLNVARQEEEMKAKEEELRNAMSKTQELVDRIKELEEKLATLSQEKNDLTIQLQAEQENLTDAEERLTQMMKAKMDLESQISDMKERLEEEESSAASLSAHKRKLEAELNDLKRDLEGLETTLAKTEKEKQARIEELEEELEAERSIRAKIEKQRSDLSRELEELTDRLEEAGGATTAQIEQNRKREAELLKLRRELEEAALQSEATASTLRKKHTDAMAEMTEHLENLQRVKSKLEKDKQVMKAEIDDLSSSMENVQKSKINAEAHVRRLEDNLAEVNARLAEMERNQAETNAVRNRLQGRLSSSPSQSNAACAALDKKQRAFDKMMAEWQQKCEELQLEVDNSQKECRMYMTENFKLKTAYEEALEQLESVKKENKTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0I7</accession>
    <name>A0A6I9Y0I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">USP12</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C19 family. USP12/USP46 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013919806.1">
        <property type="nucleotide sequence ID" value="XM_014064331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0I7"/>
    <dbReference type="GeneID" id="106547239"/>
    <dbReference type="KEGG" id="tsr:106547239"/>
    <dbReference type="CTD" id="219333"/>
    <dbReference type="OrthoDB" id="27652at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000003">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF647">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="USP" evidence="11">
        <location>
            <begin position="39"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013919806.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="42613" checksum="D5D96A076DADEB3E" modified="2020-10-07" version="1">MEILMTVSKIASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKSQPRKKESLLTCLADLFHSIATQKKKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTVADILQEEKKQEKQNGRLPNGSIDSENNNSPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPMILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEDFYGLTSDISKNSESGYILFYQSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0Z6</accession>
    <name>A0A6I9Y0Z6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Protein FAM204A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">FAM204A</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916636.1">
        <property type="nucleotide sequence ID" value="XM_014061161.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916637.1">
        <property type="nucleotide sequence ID" value="XM_014061162.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916638.1">
        <property type="nucleotide sequence ID" value="XM_014061163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0Z6"/>
    <dbReference type="GeneID" id="106544782"/>
    <dbReference type="KEGG" id="tsr:106544782"/>
    <dbReference type="CTD" id="63877"/>
    <dbReference type="OrthoDB" id="2418792at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037690">
        <property type="entry name" value="FAM204A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14386">
        <property type="entry name" value="PROTEIN FAM204A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14386:SF2">
        <property type="entry name" value="PROTEIN FAM204A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013916638.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="27358" checksum="53463A2C5FE27601" modified="2020-10-07" version="1">MWSGLLPPGVNEADVAISSDDEETVSLTENILNEKQEEKKFLDKQACDHEGKNMAENQLPSTEEQECLACLPGVSLDMWNKFQNLKKKNLDMKNQEKCQRYRRQKRKRSQKGKMKSSEEITESQESVKEKHFKELTQYFGINDRFKPLESKETAQKSGLEISIDKCVADGNIAQAEELSDRLATRELGVKIAKAVACRNFVKAKQDVEVTQEARKKKKLAWGFEAKKRWETKSNMGYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1P0</accession>
    <name>A0A6I9Y1P0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox protein aristaless-like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ALX4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916918.1">
        <property type="nucleotide sequence ID" value="XM_014061443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1P0"/>
    <dbReference type="GeneID" id="106545005"/>
    <dbReference type="KEGG" id="tsr:106545005"/>
    <dbReference type="CTD" id="60529"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000710">
        <property type="entry name" value="Paired box 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF322">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="216"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="7">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="3">
        <location>
            <begin position="218"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916918.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="38726" checksum="DDFE38BC46AA5BAE" modified="2020-10-07" version="1">MNAESCVSFCDPSAATMDSFYNAASQGSVDGSSPFRVFPGSSDKFSSAFLASKGQSFGDSGGGGGGSSGSSNNSPKRRSRYSQPECPSLDGSGAQQPQALPTAAAASSTTNTAAAFNKYHLQPPPPLYGQRGSCKSPPSGNLKLQDTSGHNGALQLSCFAKESSLGETELQPGSDSAGMDSSYLSVKETSVKTSQERASNDLPSPMDKTDSESNKGKKRRNRTTFTSYQLEELEKVFQKTHYPDVYAREQLAMRTDLTEARVQIQNPSWIGNNGAASPVPACVVPCDSVPSCMSPHAHPHTTGGVSEFLSVSGPGSHVGQTHMGSLFGSAGISPSINGYELNGEPDRKSSSIAALRMKAKEHSAAISWAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1S9</accession>
    <name>A0A6I9Y1S9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Muscle M-line assembly protein unc-89-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910745.1">
        <property type="nucleotide sequence ID" value="XM_014055270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1S9"/>
    <dbReference type="GeneID" id="106540219"/>
    <dbReference type="KEGG" id="tsr:106540219"/>
    <dbReference type="OrthoDB" id="9908116at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910745.1"/>
        </source>
    </evidence>
    <sequence length="699" mass="80315" checksum="950BC31CDADD6F5B" modified="2020-10-07" version="1">MKPINYPLIYVLEEERKKVTAEKKGVPEIKKKEVEEEKMVIHGKKDVSDIQAKRKKEEKRKTPERTVLSVVKKADTKEVVSTKDLKKPAKTPTSPQPVSVKVATPADKPKKAAHVKESETHKKEKPIEIAKVPKKHVHVKKTEPDKKEKSIEIAKLPKKAVYANKTEADKKEKPIDLAKLLKKAVLAKKPEPHKKEKPREMVKVTKKDVHENKTEPHKKEKPLETAKLLMKDIHVKESEPQKKEKPREMAKVPKKDVHVNKTESHKMEKTLETARLPMKDIHIKESDPHKKEKPREMAKLPKKDVHVKKSEPHRKEKPLETAKLPMKDIHVKESAKLPKKDVLINKTAKLLMKVIHIKESEPHKKEKPREMAKVPKKEVNKAETHKKEKPIDIAKLPKKDVHVKETAPQPKKDIHIQESAKMPKKHVRVKESETHKKEKPTETAKLPKSDVHLKETESHKKEKLAETAKLPKKDIHVKESAKVSMEDVHVNKTECHPQLANKQPNVEVIFWSSYFPEASKKGKQIETAKLPKKAVHADQTAKLPKKAVHADQTDKLLKKDIHVKESEADKKEKPIEIAKLPKKPVQASKTETDKKEKPVEIAKLPKKDIHIKETALQEKKEERPSKAGEQDEKKAEKSLREKKEKHGKTVEKTPLKVKHDKAAVRYYQCVFVNGINGYTPRYSVTQEKPADPKLKARKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4H9</accession>
    <name>A0A6I9Y4H9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dermatopontin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DPT</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dermatopontin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918898.1">
        <property type="nucleotide sequence ID" value="XM_014063423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4H9"/>
    <dbReference type="GeneID" id="106546536"/>
    <dbReference type="KEGG" id="tsr:106546536"/>
    <dbReference type="CTD" id="1805"/>
    <dbReference type="OrthoDB" id="8545119at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026645">
        <property type="entry name" value="Dermatopontin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15040:SF2">
        <property type="entry name" value="DERMATOPONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15040">
        <property type="entry name" value="DERMATOPONTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14704">
        <property type="entry name" value="DERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026772863" evidence="5">
        <location>
            <begin position="19"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013918898.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="23737" checksum="E665AB3488517F51" modified="2020-10-07" version="1">MDLILLCMSLPLFTVAWGQYGNYYHSYDDYRANDDWVNLYRQGFNFQCPHGQVIVAVRSMFSKKEGSDRLWNYACMPTPDTLGEPTECWWEEINRAGLQWYQTCSNNGLVAGFQSQYFPSVLDREWQFYCCRYSRRCPYSCWMTSEHPGHYGEDMDMMLYTYDHYIRGATTTFSGVERDRQWKFIVCRMTNFDCQFQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4W7</accession>
    <name>A0A6I9Y4W7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Selenoprotein Pb-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106546850</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919298.1">
        <property type="nucleotide sequence ID" value="XM_014063823.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546850"/>
    <dbReference type="KEGG" id="tsr:106546850"/>
    <dbReference type="OrthoDB" id="6134775at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001887">
        <property type="term" value="P:selenium compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007671">
        <property type="entry name" value="Selenoprotein-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037941">
        <property type="entry name" value="SeP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10105">
        <property type="entry name" value="SELENOPROTEIN P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10105:SF4">
        <property type="entry name" value="SELENOPROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04592">
        <property type="entry name" value="SelP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0711">Selenium</keyword>
    <keyword evidence="4 10" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026905758" evidence="7">
        <location>
            <begin position="23"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein P N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="215"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="10">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919298.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29466" checksum="B358B906D657BFCA" modified="2020-10-07" version="1">MGYLPPTTKALLLGLVISATVAEGVVVENQTRICQPAPLWKTNGIDPMAGMEGQVTVVVLLKASUQFCLTQAASLGSLREKLSGDGVANVSFMIVNEKTPSSRAMYWELKRHAPKGIPVYQQQILEPDVWQILQGNKDDFLIYDRCRKLTFHIQLPYSFLHLPYVEAAVRYTFRKDYCGNCSWYYLNSSQEVNNTASIPVMTTEAPRDKVKSLEVLKSQPEKKNQEAPNFKQNHSHHGGTNTAQPSLHSQNHSVNHHTRESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5N1</accession>
    <name>A0A6I9Y5N1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein LTV1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LTV1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913290.1">
        <property type="nucleotide sequence ID" value="XM_014057815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5N1"/>
    <dbReference type="GeneID" id="106542165"/>
    <dbReference type="KEGG" id="tsr:106542165"/>
    <dbReference type="CTD" id="84946"/>
    <dbReference type="OrthoDB" id="5852896at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="178"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="381"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="414"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913290.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="54592" checksum="6A707A747C904ABE" modified="2020-10-07" version="1">MPHRRKKPFIEKKKAVTFHVVHRSQKDPLTADDSAPQRVLLPTLKRNDEKRKEEQRKYGVFFDDDYNYLQHLKEASGPSELVPSSFPNKGRIVVSTEGEVDEIQHILAPTINLPSSVFASEFEEDVGLLNKAAPVPGPRPDLDPDIIAALDDDFDFDNPDNLLEDDFILAANKPGSLEQEKELSCLSEDEDSWEDVEEEEDGEDEEYDSEGPLSDDSDFKGKMKEFLFMEEETKSRFTEYSMTSSVMKRNEQLTLLDERFEKFFEQFDDDEIGPLDNAELEGFIQSDSARLQEVLNDYFREKAKDCVKLDDIETKKEPELPLNEEEDEIEEMVTLIIEKPKEKWDCESILSTYSNLYNHPKVIEVPKKLKSVKVSKKTGIPLDVLPQRGPTAKQTEQMQRINDSDLPRISTQPRSKDESKEDRKARKQAIKEERKERRIEKKANKSAFKQEKTRQEKELLNVKQNLQGLKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6G3</accession>
    <name>A0A6I9Y6G3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">F-box only protein 47</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FBXO47</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920648.1">
        <property type="nucleotide sequence ID" value="XM_014065173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6G3"/>
    <dbReference type="GeneID" id="106547903"/>
    <dbReference type="KEGG" id="tsr:106547903"/>
    <dbReference type="CTD" id="494188"/>
    <dbReference type="OrthoDB" id="9858120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd22112">
        <property type="entry name" value="F-box_FBXO47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056622">
        <property type="entry name" value="ARM_FBXO47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038946">
        <property type="entry name" value="FBXO47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34098">
        <property type="entry name" value="F-BOX ONLY PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34098:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24467">
        <property type="entry name" value="ARM_FBXO47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBXO47 ARM repeats region" evidence="2">
        <location>
            <begin position="225"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920648.1"/>
        </source>
    </evidence>
    <sequence length="483" mass="55691" checksum="856ECC345ABD5132" modified="2020-10-07" version="1">MGIKDYLMQCNEYLSNFWGINNEKNEIGFRMDPSTSTMQGEKSASPTRNNSTLSPNEKQNCSNAMPTYFSFMPTLGDFKGFPLEIFEFMLGYLSVKDISMMSMVSKIIRDRIINYISTPSGNRKFFPQDFHNQDQSDSAKSSILQHYSSLGLLFKRCTLLLPTKERLKYIYKRFLEIPCFQFYGCADPLHCLGLQCYGVFLQTVTAGWDEDECERVFNFLCDVTSLPQRIQRVVSSKPGRFRKLELRIRTFCRGVLLKHWILQSDSVFWLTCILKPWPSVIQARLLYLMFGPASTEDGNVDWRNMIQGPTDENNLKELADAVKLLYDTEKDEWLANDIVSLVDELAVIPQEWHLENSARFLILCGNNICFPFMASKAVDGRAIELARLVVFLALVCEKDLYCMGWSVKMMQKVCNVFSSTTEKNVFLQSIENAFAQMIMDALQLVISGEHDEEETSFLNLFHLVNAQANFHKEILYLTMNYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6U2</accession>
    <name>A0A6I9Y6U2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106548207</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013921018.1">
        <property type="nucleotide sequence ID" value="XM_014065543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6U2"/>
    <dbReference type="GeneID" id="106548207"/>
    <dbReference type="KEGG" id="tsr:106548207"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002997">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF73">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026803991" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="8">
        <location>
            <begin position="61"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="68"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="274"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013921018.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="36489" checksum="8D57CF09A68619E5" modified="2020-10-07" version="1">MARVCLLLLCLKMCEIHTQEGRWETNWKRNDSDVMNDLLDQWKRNDSNALDEDWKQRLPKGIIIGVRKGGTRALLEMLSLHPQVAAAHSEIHFFNVEENYQRGLDWYSQQMPNSHSTQITVEKTPGYFASLEAPERIYTMDRSVKLLLIVRDPVERLVSDYTQILYNRKARQKPYQSLEEILRRGQELNTDYKAMQRSLYALHLARWLEFFPTTQIHVVDGGGLIRQPLSEMRHVEQFLGLEPYLGPDNFYFNETKGFFCLQAAGERHCLDPSKGRPHPTVDELLLERLCTYFSQHNEDFFAMVGRTFNWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7F6</accession>
    <name>A0A6I9Y7F6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-ESSS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase ESSS subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NDUFB11</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Complex I is composed of 45 different subunits. Interacts with BCAP31.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB11 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915090.1">
        <property type="nucleotide sequence ID" value="XM_014059615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7F6"/>
    <dbReference type="GeneID" id="106543567"/>
    <dbReference type="KEGG" id="tsr:106543567"/>
    <dbReference type="CTD" id="54539"/>
    <dbReference type="OrthoDB" id="5917019at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019329">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ESSS_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13327:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 11, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13327">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE ESSS SUBUNIT, MITOCHONDRIAL PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10183">
        <property type="entry name" value="ESSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013915090.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="17103" checksum="B3890752D5AEF6F5" modified="2020-10-07" version="1">MSTMAALRRLAGSQRLLGLPVRAVSSGQTANTTVSARISPASRPMDVVEWHPMEKEEEKSPFVKNPDFHGYSDDPVTDVQNMRATFFFGISVCLVLGTTYLYYLPDHKNLHWARREAERRVKERTEQGLPILSSYYYDPSKIVLPPEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7P5</accession>
    <name>A0A6I9Y7P5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MIT domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915535.1">
        <property type="nucleotide sequence ID" value="XM_014060060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7P5"/>
    <dbReference type="GeneID" id="106543953"/>
    <dbReference type="KEGG" id="tsr:106543953"/>
    <dbReference type="OrthoDB" id="19553at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061952">
        <property type="term" value="P:midbody abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.30">
        <property type="entry name" value="MITD, C-terminal phospholipase D-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052817">
        <property type="entry name" value="MIT_domain_contain_protein1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032341">
        <property type="entry name" value="MITD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038113">
        <property type="entry name" value="MITD1_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21222">
        <property type="entry name" value="MIT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21222:SF1">
        <property type="entry name" value="MIT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16565">
        <property type="entry name" value="MIT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MITD1 C-terminal phospholipase D-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915535.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13770" checksum="B9D49EF082593715" modified="2020-10-07" version="1">MLVKGPCRMKTIHLLTSHEEGHGKNQQMGSLEEIQQSLRNFGITLDISYSSSIHDREIRFNNGWMFKIGRGLDYFKKPQGRFCLGYCDFDLRPCHETTVDIFHNNYTKKTIPSETTPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8Y0</accession>
    <name>A0A6I9Y8Y0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyl-CpG-binding domain protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Methyl-CpG-binding protein MBD5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106545602</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Non-catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1). Important for stability of PR-DUB components and stimulating its ubiquitinase activity. As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3. The PR-DUB complex is an epigenetic regulator of gene expression, including genes involved in cell growth and survivability. MBD5 and MBD6 containing complexes associate with distinct chromatin regions enriched in genes involved in different pathways. Heterochromatin recruitment is not mediated by DNA methylation. The PR-DUB complex is an epigenetic regulator of gene expression, including genes involved in development, cell communication, signaling, cell proliferation and cell viability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Core component of the polycomb repressive deubiquitinase (PR-DUB) complex, at least composed of BAP1, one of ASXL1, ASXL2 or (probably) ASXL3, and one of MBD5 or MBD6. Distinct combinations of ASXL and MBD proteins may preferentially bind specific histone modification marks. The PR-DUB core associates with a number of accessory proteins, including FOXK1, FOXK2, KDM1B, HCFC1 and OGT; KDM1B specifically associates with ASXL2 PR-DUB complexes. Interacts (via MBD domain) with ASXL1, ASXL2 and ASXL3 (via PHD domain); the interaction is probably direct, mediates association with other PR-DUB complex core components.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917710.1">
        <property type="nucleotide sequence ID" value="XM_014062235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8Y0"/>
    <dbReference type="GeneID" id="106545602"/>
    <dbReference type="KEGG" id="tsr:106545602"/>
    <dbReference type="OrthoDB" id="641149at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010369">
        <property type="term" value="C:chromocenter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20141">
        <property type="entry name" value="PWWP_MBD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000035">
        <property type="entry name" value="Methyl-CpG binding domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16112">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, DROSOPHILA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16112:SF18">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="PWWP" evidence="11">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="318"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="373"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="492"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="509"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013917710.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="57784" checksum="DC77DAF3B598254F" modified="2020-10-07" version="1">MFPSNQQQQLLQGYQNLQGFQGQLPVPGPPNNPNPMACLFQNFQVRMQEETAFLNKRIISQIGMPPVPESSSAAIPPFQDTVCNLQQRTEPSVGQQAKDINLAAQSDGSVDAIYKAVVDAAGKGMQVVITTAVNSTTQMSPIPALSAMSAFTASIGDPLNLSSAVNAVIHGRNIGISDHEDRARNIARGARLLKNSEHLKNSSDGDSLEYYKPTGCNTPKKQWEGEQSPGGEMNRWKCEEVLDHSSHILSSPCHERSNNISTLPLVQGKQHPVLLPQRNCHSDKMVEENFRYSNYKRTMMSFKERLENTVERCAHINGNRPQQSRGFGELLNPSKQDLGVDEQSPSSSNSFENSLIKDYIHYNGDFNAKSINGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWSQIKGLSSWPGKLVREDDVRNSCQQTTEDGKVEPDKLKTLTEGLEAYNHVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGNVHQIPQGDRQLKPPKPKRRKISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9A9</accession>
    <name>A0A6I9Y9A9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GPI-anchored protein PB15E9.01c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106546102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918355.1">
        <property type="nucleotide sequence ID" value="XM_014062880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9A9"/>
    <dbReference type="GeneID" id="106546102"/>
    <dbReference type="KEGG" id="tsr:106546102"/>
    <dbReference type="OrthoDB" id="10070537at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEA" evidence="2">
        <location>
            <begin position="672"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="511"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="636"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918355.1"/>
        </source>
    </evidence>
    <sequence length="873" mass="94054" checksum="4DF8ADC3B0C44E6A" modified="2020-10-07" version="1">MARSHLDPEDSTAIHPYEESVTASPPIHSLVALVGSSTQPSTTDSILQASRQLGDNFTTPLTSSSPPSSSTVEAGFSPDAANDGSDRMVPRSIDTIPTSVAGTPRNQVSLGVTQLAFATATVGPLKEANPSLEPPSSLLKEHIESFPTTLSFGTSKEDEISLVSHSILNSSLVEAIKLEPKDNTSSIVESLVGPTVSSIDGPISVSFQTNLLDTTDDRKPLETQHYGRKRQISASMEEIPTSKSPPTAMEADSVRKLTNVQPVDALPKRGTGAPPLSPLSSTTLMSKMRQTSKDSSEPETITDFLVTKGFSLGTTKTDSAVPSFPYLDIGDVLHPIGATSTRLTYTDLLEGYTTSPSVESLGSSSVEQKLSSPSQSHPTVSLPGLIEFDRSSVPPSHRSLSSSMTSIPFSGISVPETTSKTFINITEGHLVRIGQVMPRGTKSFSATPLPLESRSTGGGIALSLPTKVPRVGLRTSSEMATLENAMGHQVTTTSTANNIQAIGRSTLPIRSSSSTTSSNSSVPAYPSSVLETRSSQGTPIFSASATLWTTQRTLPVEPKTTCRSISCPVSISTPPQNNRSAQSMTPQPFLARKDLSTISGTRSPKESTSLAGASGSRSEERDATALPRTKIVTRVQQDPSSQQPSSKFTTSPFNPSAKEDLTLGTFASTVPAVRVLSLWFRLLKINYTNSLMNKSCESYRRLEKEIKLTLKKILSTYENFLHAKVLRFLNGSVVVESQVAFQATGLLPTPSDIIRTIVTEVESRKIDAFFDWRIDVRSLHLNGFGLKNLEPENLAVFFSLLELGSTFGDENFAQEHLESLKIKMKQLLGTRYAVHLISLIDNRYVVTFLGRINSGHFSRLDTLTNPKQLHLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA31</accession>
    <name>A0A6I9YA31_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histone deacetylase complex subunit SAP130 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SAP130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP130 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919715.1">
        <property type="nucleotide sequence ID" value="XM_014064240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA31"/>
    <dbReference type="GeneID" id="106547173"/>
    <dbReference type="KEGG" id="tsr:106547173"/>
    <dbReference type="CTD" id="79595"/>
    <dbReference type="OrthoDB" id="10048604at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024137">
        <property type="entry name" value="His_deAcase_cplx_SAP130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031963">
        <property type="entry name" value="SAP130_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13497">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13497:SF3">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16014">
        <property type="entry name" value="SAP130_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP130 C-terminal" evidence="8">
        <location>
            <begin position="649"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="567"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="637"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="848"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="637"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919715.1"/>
        </source>
    </evidence>
    <sequence length="1071" mass="113236" checksum="F632867EF70766E8" modified="2020-10-07" version="1">MSSQQFPRTGVPPPSLGPAPPISAGGPAGLINPAATGEAFLPRTMFHGIVNDEPNRELEMAPRDHVVPAGSLQSREEKQETVVVRPYPQVQMLTQHHTVPSGVPVTVAAPPTHLAPAVPLTFSEGLIKPPLKPAIPSRPIAPAPPAPPSTLSGPTKVPGQVTVTMENSIPQASAIPVATISGQQGHPNSVHHIMTTNVQMSIIRSSAPGPPLHIGASHLPRGAAAAAVMSSSKVTTVLRPASQLPNAATAQPAVQHIIHQPIQSRPAVTTSSTIPPAVVATVSATRAQSPVITTTTTHATESTLSRPALSIQQHPPSAPISIQRPAQPRDTATRITLPSHPAIGTQKQQLHPMAQKTIFSTGTPVAAATVAPILATNTIPSVTTAGSIPHTQAAPTTIVTVTMPSHSSHATAVTTSNIPVAKVVPQQITHTSPRIQSDYTAERSNLIPISSHRASPNPVAMEARNDNRQSVPVQFQYFLPTSYPSYPLAAHTYTPITSSVSTIRQYPVSAQAPNSAIAAQTGVGVASTVHLNPMQLMTVDASHARHIQGIQSTPIGAQGIQPAPISAQGIQSTPIGTQGLHPATPIGTQGLQTAPLGPQQPQTETKTSVVLADGATIMANPISNTFNSASASTTVVQTHSQSANSSTSAQGSSPRPSILRKKPTTDGLAVRKSLIPPQPTEVAGPRVESSVRSASGSPRPAGVKPKPEIHVSMATPVTVSVEAVSIQSNEQPPVSVPSSQQPQSAIPTIITAASPPSQPTAALSSIPGTLPAPPPTSATVVTVPPPPSTISGILSTVLGPVVPEIKIKEEVEPLDIMRPVSAVPPLSTNSVSSSVTLLANNLTVPQSDLLPGASPRKKPRKQQHIISTEEGDMMETNSTDDEKSTTKSLLIKAEKRKSPPKEYIDEEGVRYVPVRPRPPITLLRHYRNPWKAAYHHFQRYSDVRVKEEKKAMLQEIANQKGVSCRAQGWKVHLCAAQLLQLTNLEHDVYERLTSLQEGIIPKKKAATDDDLHQINELIQGNMQRCKLVMDQINEARESMLKVLDHKERVLKLLNKNGTVKKVSKLKRKEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAR4</accession>
    <name>A0A6I9YAR4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exosome component 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">EXOSC10</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920880.1">
        <property type="nucleotide sequence ID" value="XM_014065405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAR4"/>
    <dbReference type="GeneID" id="106548088"/>
    <dbReference type="KEGG" id="tsr:106548088"/>
    <dbReference type="CTD" id="5394"/>
    <dbReference type="OrthoDB" id="9909598at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920880.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13876" checksum="AB2C94F6D586D129" modified="2020-10-07" version="1">MQRPFVFPWLCFQQEHATPKREAEDQNPATETLKRKKAKPEAPQPEADPAASCFTPFDYSKSDFRVFAGSSKSKPPRQFNPAIQAAGNKKFSGASKSQQPAGTKSMSFTAGKSNRGFRHNWPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCV6</accession>
    <name>A0A6I9YCV6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein FAM110A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FAM110A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM110 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922001.1">
        <property type="nucleotide sequence ID" value="XM_014066526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCV6"/>
    <dbReference type="GeneID" id="106549014"/>
    <dbReference type="KEGG" id="tsr:106549014"/>
    <dbReference type="CTD" id="83541"/>
    <dbReference type="OrthoDB" id="10028183at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025740">
        <property type="entry name" value="FAM110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025741">
        <property type="entry name" value="FAM110_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025739">
        <property type="entry name" value="FAM110_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14758">
        <property type="entry name" value="AGAP005440-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14758:SF4">
        <property type="entry name" value="PROTEIN FAM110A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14160">
        <property type="entry name" value="FAM110_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14161">
        <property type="entry name" value="FAM110_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosome-associated FAM110 N-terminal" evidence="4">
        <location>
            <begin position="14"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Centrosome-associated FAM110 C-terminal" evidence="3">
        <location>
            <begin position="248"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013922001.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="39429" checksum="FD1C84DE7B12A41B" modified="2020-10-07" version="1">MPVETLQASRAMSTVSATAPFTSAMPFRILNKGPEYFRRAPASTSRKLSAVERLEADKAKYVKSQQVATTKQDPIRPLVLKQPLFTPGVRRAIFTPNRKVPQVGRRGEGGGGPKSSLNLEILNNLINFCDSPLAFPKPDRSPSDSPWRAKVHSRNLDSLPSTPKKKPATDMSKLSQSSAASKPSSTVAVRRVDVRPCGVSPARGTPAIQLSPLPAPLAPPQNLPATPLSCPSPRGEPRTDSAKPQTLLHRSKSDLSDRYSRATADLERFFNYCGLDPEDVGDMEMERFTRASSDIVSIKFHSVSTSSSVGTHSRRSTVTVEDKQAERMPYGISIIERNARVIKWLYGLRQAKEAQKVSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCX2</accession>
    <name>A0A6I9YCX2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Isocitrate dehydrogenase [NAD] subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">IDH3A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:23633"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921909.1">
        <property type="nucleotide sequence ID" value="XM_014066434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCX2"/>
    <dbReference type="GeneID" id="106548931"/>
    <dbReference type="KEGG" id="tsr:106548931"/>
    <dbReference type="CTD" id="3419"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000003">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 13" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="14">
        <location>
            <begin position="33"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013921909.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="40079" checksum="A03DBCA07951B99E" modified="2020-10-07" version="1">MATCSWVPKVFRILGGFRIQKQVTRNFASNPQTVTLIPGDGIGPEISAAVAKIFEAAKVPVQFEERKVTAIQGPGGKWMIPQDAKDSMDKNKIGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDIVTIRENTEGEYSGIEHLIVDGVVQSIKLITEDASKRIADFAFEYARNNQRSKVTAVHKANIMRMSDGLFLRKCREAAENCKDIKFNEMYLDTVCLNMVQDPSRFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDLANPTALLLSAVMMLRHMGMHDFAMKIETACFDTIKDQKVLTKDLGGNAKCSEFTAEICRRVRAME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE23</accession>
    <name>A0A6I9YE23_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Four and a half LIM domains protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">FHL2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922417.1">
        <property type="nucleotide sequence ID" value="XM_014066942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE23"/>
    <dbReference type="GeneID" id="106549323"/>
    <dbReference type="KEGG" id="tsr:106549323"/>
    <dbReference type="CTD" id="2274"/>
    <dbReference type="OrthoDB" id="274660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070885">
        <property type="term" value="P:negative regulation of calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09426">
        <property type="entry name" value="LIM2_FHL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09431">
        <property type="entry name" value="LIM3_Fhl2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09433">
        <property type="entry name" value="LIM4_FHL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000013">
        <property type="entry name" value="Four and a half LIM domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000030">
        <property type="entry name" value="Four and a half LIM domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000048">
        <property type="entry name" value="Four and a half LIM domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205:SF3">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="148"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="268"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922417.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="37221" checksum="8AFAC7D6FBB4B1FE" modified="2020-10-07" version="1">MLELILGELFSCRVNALGCFNHFSGYFCACSSNKLDGFETECMSLFIAIEATNEFGSPRLTVRLCSCNLVLSVLVYNAVVNLSQGYGVFLHVGLCGLGFLQDLSYKDRHWHETCFHCFQCKNSLVDKPFAAKEEHLLCTECYSNEYSSKCSECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQFAMQCVQCKKPITTGGITYREQPWHKECFVCTGCKKQLSGQRFTSRDEFAYCLNCFCSLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPECGKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YED7</accession>
    <name>A0A6I9YED7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Netrin receptor UNC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">UNC5A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the unc-5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922370.1">
        <property type="nucleotide sequence ID" value="XM_014066895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YED7"/>
    <dbReference type="GeneID" id="106549296"/>
    <dbReference type="KEGG" id="tsr:106549296"/>
    <dbReference type="CTD" id="90249"/>
    <dbReference type="OrthoDB" id="5973910at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033564">
        <property type="term" value="P:anterior/posterior axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000001">
        <property type="entry name" value="Unc-5 netrin receptor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000002">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000008">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000003">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000037">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000039">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057755">
        <property type="entry name" value="UNC5A-D-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033772">
        <property type="entry name" value="UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF4">
        <property type="entry name" value="NETRIN RECEPTOR UNC5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25609">
        <property type="entry name" value="Unc5_NetrinR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17217">
        <property type="entry name" value="UPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 14" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027140627" description="Netrin receptor UNC5" evidence="14">
        <location>
            <begin position="37"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="166"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="16">
        <location>
            <begin position="507"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013922370.1"/>
        </source>
    </evidence>
    <sequence length="908" mass="99832" checksum="DEE34860C050711D" modified="2020-10-07" version="1">MAGWLQLGASARVGTGLWQVLLAGTILTSHIHPNDAQQSATVPIPVPGSGSDLLPHFLLEPDDVYIVKNKPVTLTCKAVPATQIYFKCNGEWVHQGDHIIQRSKDKSTGLPLMEVRIDISRQQVEKIFGLEEYWCQCVAWSSSGTTKSQKAFVRIAYLRKNFEQEPVAKDVSLEQGIVLPCRPPEGIPPAEVEWLRNEELVDPTLDPNYYITLEHSLVVKQARLADTGNYTCVAKNIVARRRSSPAAITVYVNGGWSTWTDWSACSTSCGRGWQKRSRSCTNPAPLNGGAFCEGQNVQKSSCATLCPVPGGWSAWSLWSVCGADCTHWRSRECSDPVPRNGGDECQGPELETRNCTSELCTHETGGSEDVALYVGLIAVAVCLVLLLLVVTLVYCRKKEGLDSDVADSSILTAGFQPVGIKPTKADNSHLLTIQPDLSTTTMTYQGSLCSRQDGPGKFQIGNGHLLSPLGSGRHTLHHSSPSADPVDFVSRFSTQSFFRSLPRGGANMAYGTFNFLGGRLMIPNTGVSLLIPPDAIPRGKIYEIYLTLHKQEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAIDHCVEPNPETWNLRLKKQSCEGTWEDVLQLGDPGSSELFHCQLEPQACYVFTEQLGRFALVGESLSMAASKRLKLVLFAPTTCTGLEYDVRVYCLSDTRDALKEVVQIEKQMGGQLIGAPRILHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHLWSGLQPYLHCTFTLERLSTNTCQLACKIWIWQVEGDGQSFNIDFNIAKDARFSDWLPPDNEAGSPALVGPSAFKIPFLIRQKIISSLDAPVARGADWRALAQKLHLDSHLSYFASKPSPTAMILNLWEARHFPNGNLNQLAAAVAEVGKQDSSLFMVSEAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEE9</accession>
    <name>A0A6I9YEE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Caskin-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CASKIN2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">May not bind CASK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922380.1">
        <property type="nucleotide sequence ID" value="XM_014066905.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549302"/>
    <dbReference type="KEGG" id="tsr:106549302"/>
    <dbReference type="CTD" id="57513"/>
    <dbReference type="OrthoDB" id="6156898at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09497">
        <property type="entry name" value="SAM_caskin1_2_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09498">
        <property type="entry name" value="SAM_caskin1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12063">
        <property type="entry name" value="SH3_Caskin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000032">
        <property type="entry name" value="caskin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000053">
        <property type="entry name" value="caskin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000139">
        <property type="entry name" value="caskin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000062">
        <property type="entry name" value="caskin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000028">
        <property type="entry name" value="caskin-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000042">
        <property type="entry name" value="caskin-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033635">
        <property type="entry name" value="ANKS1/Caskin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032232">
        <property type="entry name" value="Caskin1-CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035497">
        <property type="entry name" value="Caskin1/2_SAM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035498">
        <property type="entry name" value="Caskin1/2_SAM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035499">
        <property type="entry name" value="Caskin2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032117">
        <property type="entry name" value="Caskin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF18">
        <property type="entry name" value="CASKIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16907">
        <property type="entry name" value="Caskin-Pro-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16632">
        <property type="entry name" value="Caskin-tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16600">
        <property type="entry name" value="Caskin1-CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0728">SH3 domain</keyword>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="281"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="509"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="578"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="403"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="711"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="804"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="903"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="711"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="738"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="811"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="826"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="953"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1021"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1032"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1064"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1086"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1116"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1129"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1150"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1186"/>
            <end position="1199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013922380.1"/>
        </source>
    </evidence>
    <sequence length="1475" mass="159686" checksum="22CD613B76F338C3" modified="2020-10-07" version="1">MGREQELIQAVKNEDVPGVQKLVAKIKVSKSKLLGSTKRLNVNYQDVDGFSALHHAALVGSLELISLLLEAQAIVDIKDTNGMRPLHYAAWQGKVEPVRLLLRASASVNMASLDGQVPLHLSAQYGHYEVSEMLLQHQSNPCLINKAKKTPLDLACEFGRLKVAQLLLNSHMCVALLEGQFKDTSDPNYTTPLHLAAKNGHKEIIRQLLKAGIEINRQTKTGTALHEAALYGKTEVVRLLLQGGIDVNIRNTYNQTALDIVNQFTTSHASKDIKQLLREASGILKVRALKDFWNLHDPTALNIRAGDVITVLEQHADGRWKGHIHDAQKGTDRVGYFPPSIVEVISKRTGLTLSRMVSTHQCQSHAKGLQVTTATPPGGLQLHSDTSPPQAPLSLPTGWGHLTLTRTAPAPENPAGDRNSVGSEGSIGSIRSAGSGQSTEGTNGQFMGLLIENVKPLLPGGEDLQQHILSSNSHNGQTSSLGTQTHQNFSNDRPFSHQYLHPEKLLEGKDAAAIYNWLSNFQLECYTANFLNAGYDVPTISRMTPEDLTAIGVTKPGHRKKISTEIGQLSIAEWLPNYIPDDLMEWLSAIGLPQYHKKLVSNGYDSINIVTDLTWEDLQEIGINKLGHQKKIMLAVKRLTDLCKSQSQAKVNTLRRKIPGTLEIVTIETVETGECHSPLTPKMTTFQDSELSHELQTAMSNSCQGTLTIKSTQGISRSQESIGARSRGSGHSQENMLSQSTSSSHSQESLDSGEGSSGSSGGHSFTQLHPVENSAITQGRPNPDHYGKFIPLLNGPDGVNGYSSSCVGSPLRERNLPEGMDHFHRSSASPEPSSPLSVPSPCTFPHAASKGTAPYVFMYPHVSLKAQLAPPPFMPEQAKPSTHLSVQRTALQSTAQKAFSYLHSHCGPTEPPQVPPKPRPEQQNGEGFKHKKRSQSLNRYALSDGEPDEEEPASSSVGSYATLTRRPGRSQVARTQPPSEAKVLRSQSFAIRARKKGPPPPPPKRLSSVSSPLAVDAEFEQGQQTQNGSSLSKDDGDNTNDRACLEKNTAAKLDRPIYDGSPAQPLQSSQIPSCGQEGTKKGGLHNSVQGGLVNTNTLNGRSQVLEGNTPRRRTFSESAVTMTEALQHNLEDIRSDTEEDGPVEKDDSAKSPCSSQNSSSECIPFAEEGNLTIKQRPKPLGQMKNDAPAPDSDSAPAQSNGKESQEDSAKEGPMLEFNLTESDTVKRRPRCKEREPLQNVLKAFGMTTAPQYAQAQAVSISGPSLHMMEALPTTGPLEDDHMAFQIVEIEKSILSLEKGISKLPVSLKPASPGTSHSERHGGSAQLRIPASVTDMTAMPTSIASTKLVFSGPKTIYQQVLPNSHHPGPSRAAHEMASGASSPYPIKPGLSNKPAFNFGVPHPPRPSSLCPGVIQTQQRLEQTSSSLASTLEAAEKEIIAEEAESCYGTTHSAKNILEDISNMFNDLAEQLDAMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YER6</accession>
    <name>A0A6I9YER6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein strawberry notch homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106549407</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922534.1">
        <property type="nucleotide sequence ID" value="XM_014067059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YER6"/>
    <dbReference type="GeneID" id="106549407"/>
    <dbReference type="KEGG" id="tsr:106549407"/>
    <dbReference type="OrthoDB" id="421838at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000282">
        <property type="entry name" value="Strawberry notch homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057332">
        <property type="entry name" value="SBNO_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF8">
        <property type="entry name" value="PROTEIN STRAWBERRY NOTCH HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25373">
        <property type="entry name" value="SBNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Strawberry notch AAA" evidence="7">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Strawberry notch AAA" evidence="7">
        <location>
            <begin position="662"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="SBNO alpha/beta" evidence="8">
        <location>
            <begin position="931"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="626"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="631"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013922534.1"/>
        </source>
    </evidence>
    <sequence length="1027" mass="114942" checksum="7ECC846DDD375CF9" modified="2020-10-07" version="1">LPNGDRAGFLIGDGAGVGKGRTIAGIIYENYLLGRKRAVWFSVSNDLKYDAERDLRDIGAKNILVHSLNKFKYGKISSKHNGSVKKGVIFATYSSLIGESQSGGKYKTRLKQLLHWCGDDFDGVIVFDECHKAKNLCPVGSSKPTKTGLAVLELQNKLPKARVIYASATGASEPRNMAYMNRLGIWGEGTPFREFSDFIQAVERRGVGAMEIVAMDMKLRGMYIARQLSFSGVTFKIDEVHLSQHYVKMYNKSVKMWVSAREKFQQAADLIDAEQRMKKSMWGQFWSAHQRFFKYLCIASKVKRVVQLAREEIKNGKVSDLSSWNDRKPKIPKENTLELRELREEMTREVKKARKTGGLAGSSSDESFSESEASENEESDNESSRFLSSGDDDDFNPFRDESSEDDEDDPWLIRKEHKKNKEKKKKKSIDPDSIQSALLASGLGSKRPSSLSSTAVTPPTNVSGKSPKEGVLTCFGCQEVSAWQRKVQPPSTTTFVLNQINQLPTLGTTLVMSKTTPVTSTRQTITVAKIIQTSTTTRPSVTLPVARNTLTTPVPKDQIQLKDLLKNNSLNELMKLKPPPNIAPPVATAATDMSNGAVKKETSTKEVTRIWVNDLKMRSFSPTMKVPVTKEEEEPEEEDEEEMGHAETYAEYMPIKLKTGLRHPDPVVETSSLSSVTPPEVWYKSSIPEESIDSGWLSALQLEAITYAAQQHETFLPNGDRAGFLIGDGAGVGKGRTIAGIIYENYLLGRKRAVWFSVSNDLKYDAERDLRDIGAKNILVHSLNKYGRNALEIVMKSIVNLDSPLVSPPADYPGEFFKDVRQGLIGVGLINVEDRSGILTLDKDYNNIGKFLNRILGMEVHQQNALFQYFSDTLNAVIHNAKKNGRYDMGILDLGSGDEKVRKADAKKFLTPGYSTSGHVELYTISVERGMSWEEATKLWSEQRGPEDGFYLSLQIRNSKKTAILMKEVNPKKKLFLVYRPNTGKQLKLETYSEIKKKYKKVSSDDAVTHWVEQYTSSADTCTHAYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFH4</accession>
    <name>A0A6I9YFH4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Major histocompatibility complex class I-related gene protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106549729</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922922.1">
        <property type="nucleotide sequence ID" value="XM_014067447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFH4"/>
    <dbReference type="GeneID" id="106549729"/>
    <dbReference type="KEGG" id="tsr:106549729"/>
    <dbReference type="OrthoDB" id="8936120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000001">
        <property type="entry name" value="H-2 class I histocompatibility antigen, alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
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        <location>
            <begin position="1501"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1516"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1557"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1576"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1589"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1623"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1646"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924418.1"/>
        </source>
    </evidence>
    <sequence length="1689" mass="187778" checksum="C02F9FA7E031C12A" modified="2020-10-07" version="1">MADDRKDEAKAPHWTTGQLAEASSHPHAAEMKEQSGPGEGLVRSSNGFPYKEDEAGEYGAHGPQTSYSRTKENGINGELTTGEKDTAEEVSARIVQVVTAEAVAVLKSEQEKEAPFKDQPAQLPLAVEETANLPPSPPPSPASEQTGTLEEASKMEIHGQEGTSLFAATPLSTKEEEKGRESSEHPAQDALVPQPAKTETIEKREPQLTGEEMTFPSRLGETLDATLQEKEEKTSTPQEQRDLLTELHKKSTLDEKATETQKSATEASPLESKDDQITVTKNDFTSPASIGSSSLSESIADVGTVTSAFFPETLPGVRHTSEPEESSKTTEKSAITEITKDLSKDQTMSKGQMKIQEEDFAVVGPSASQKAIVEQTPSQVPLWEEEKDMTEDESDEEKYSMSSKQKLRELDDQPFAVCDQKPTVLGKDALGKDVDPKEAYDAPKTEDLGITGESTAWNVKKDEKTPGAEVMADRFSKDFSPEKYEEHLDTAALHETIEKSPSEAHKEPQEDKRSEKEGKGEVFPVADSDPHQMRTEEDKSGMSTYFETSTLKDEAVKSDKVQQGNDYYELSDSKDQTYDIDHKNSLVSEKETDEYQPEAHRQPSPPAHQIGYSTLAQSFPEDEAKEPSSPPERMYTIDPNVYGDKRELHNKNKDDLTLSRSLGLGGRSAIEQRSMSINLPMSCLDSIALGFNFGRGHDLSPLGSDILDTASGDEGDDYLPATTPLIEKAPRFPTESREDDDEEEEAEEIAEEEAEEEERIEESKFKESGEIQVEPLYESPLLAKDYYKNGTVMAPDLPEMLDLAGTRSRVASVSADTEVAQRKSAPSEVVVEDSSAVQQLMTDEDHMTLRADSQVEDLGYCVFTKYTVPLPSPVQDSENLAGESCSYEEKMRRGVVTDLSLIEVKLAAAERSCEGKEVSGDSPVWGQDFEQERKAIDKLDTVLEKSEEQADAKEVSFPEDTEHEIKLGAELADVNDKTLVEPEKKSSLIKEEENAIHVAANIDKAELSCKPDYNAIKHDMDSAARQVEQEYQSKLGGHITELVSEQNKEDPEKILFALHQEPDLCATLESNNIKDAAKLSEIEIKEKGPKPDLVHQEAVDKEESYESSGDNGQVQEGLSGEISKPPEGKIQREIPPLPEEEHAEKSIPKVIQEEPAETQMESIMEHKVDQADHVEITETEKVEKLPHDLKYEKEEKVEEEQKEEIKQTEEQEEEVKHTKEEIKYREEHEKEIKNVEQQEDAKHVKEPEVEIEKDEYEEKTKEEEEEEIKQAEGQEEEKEKEEETSKQTSKDEDLESPSEEVCKAIPVEIPELKGVIESVVTVEDDFITIVQTTIDEGEANSHSVRFAETAQPETAGEGPCTEELDMEAEVIAHVEADSEEKSLEPPGSAEQEEIPATDYKTETYDDYKDETTMDDSVLDTDSIWVDTQDDDRSIMTEQLETVPKEEKSERELRRPSLDKHKKEKPLKTGRGRISTPERKIAKKEPSTVSRDEVRRKKAVYKKAELSKKAEVQAHSPSRKIILKPAIKHTRPTHHSCVKRKQTAAGGEINQMPGVFKQAKEKLSDGITKSPEKRSSLPRPSSILPARRGISADRERDENSFSLNTTISSIRRTTRSEPIRSRTGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYSRTPHTPGTPTSAILVPTEKKVAIIRTPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJW5</accession>
    <name>A0A6I9YJW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase RNF170-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106550647</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924060.1">
        <property type="nucleotide sequence ID" value="XM_014068585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJW5"/>
    <dbReference type="GeneID" id="106550647"/>
    <dbReference type="KEGG" id="tsr:106550647"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16553">
        <property type="entry name" value="RING-HC_RNF170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038896">
        <property type="entry name" value="RNF170"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22894">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22894:SF2">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="31"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013924060.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="24340" checksum="FF4E9895E1E32D88" modified="2020-10-07" version="1">MSSLEKNCVPSSSKIERLQQWQQCYPSDLSCPICLQTANFPVETNCGHFFCGYCLIEYWKHGSWLSAINCPLCRQKVVFLYTCSENQPDKKNKRTFYDIRTYNKRFSGQPQSFIDHLYDMPFLLPVMLRGIFTLAALMWIFLFRVVVCFFGTIMCTTCPIEGTLEPLCGILHTADNLAVIILLLISFINIGPQMESEGANRTISREETITVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK77</accession>
    <name>A0A6I9YK77_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Ras and EF-hand domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RASEF</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds predominantly GDP, and also GTP. Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Interacts with the dynein-dynactin complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924642.1">
        <property type="nucleotide sequence ID" value="XM_014069167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YK77"/>
    <dbReference type="GeneID" id="106551141"/>
    <dbReference type="KEGG" id="tsr:106551141"/>
    <dbReference type="CTD" id="158158"/>
    <dbReference type="OrthoDB" id="9879408at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000019">
        <property type="entry name" value="RAS and EF-hand domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001348">
        <property type="entry name" value="Ras and EF-hand domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="186"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="264"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013924642.1"/>
        </source>
    </evidence>
    <sequence length="737" mass="82876" checksum="B1EE749AB35B0AAA" modified="2020-10-07" version="1">MGSSAGEQVDLDEGGSLRSLFLACDVNGSGRIERDDFGSLCAELQVRPAEAEAIFQRLDTDRDGAITFREFVRGFRGIAQPQRRRSDREELPGEESDAFGAFAEIVAAAAAARGSAVGPSQAWKEFELRLGEEAGFIPRQEQVCILYQNINIVEPRLIQPYENVIKNFIREIKLQSTEMETMAIAVKRAQDKAAMQLGELEEEMEQRIQATEHKVKKEEKRKAEEALNDLKRQYETEVGDLQVTIKKLKMLEEQSKHINQKEDLAVLKRRIHDLILENQKLKKDLMEAQTNIAFLQSELDALKSDIADQSLNSERDLDMIREYTEDRDSLERQIAILQSANRKLHDSNDGLRSALENSLSIYNRSLRLTNISPGNTMSRSSPKCGRGHSPQNPQYDRLSHSSYVDEEYDSLALCDPMQRINCEVDSLPESCFDSGLSTLRDSNEYDSEVESKHQRTSERTQCLQESFPGDASDTDVPEIRDEEVYSPDDVNTVLCWKPDQSVSRSSSGVSTRKHIPAFSPQVDSIHSNSRYPSSDKAYKIVLAGDAAVGKSSFLMRLCKNEFRGNTSATLGVDFQMKRLIVDGEPTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWVDMISDATHENIPIMMVGNKSDLRQTASEEGLTCVPLSYGEKLAMAYSALFCETSAKDGSNIVEAVLHLAREVRKRSDSKDDDKTVTKLAGTSLKKSSLTKNCCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK99</accession>
    <name>A0A6I9YK99_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium voltage-gated channel subfamily H member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">KCNH3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924581.1">
        <property type="nucleotide sequence ID" value="XM_014069106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YK99"/>
    <dbReference type="GeneID" id="106551094"/>
    <dbReference type="KEGG" id="tsr:106551094"/>
    <dbReference type="CTD" id="23416"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000061">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003950">
        <property type="entry name" value="K_chnl_volt-dep_ELK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF481">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY H MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01465">
        <property type="entry name" value="ELKCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="352"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="488"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="705"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="553"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="744"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013924581.1"/>
        </source>
    </evidence>
    <sequence length="792" mass="85554" checksum="B9B175A7DC6ED127" modified="2020-10-07" version="1">GVFGEKPALPEYKVAAARKSPFILLHYGTFKAGWDGLILLATLYVAVTVPYSVCFNGTKDERLPETSRAPPSVCDLSVEILFILDIVLNFRTTFVSKSGQVVFDPHAIFLHYLTRWFVLDLLAALPFDLLYAFKVNVYFGAHLLKTVRLLRLLRLLPNLDRYSQYSAVVLTLLMTVFALLAHWVACVWYFIGQREVESSPSQLPGIGWLQELARRLETPYYLAQRNSSGEEEPTNQTSSLNGTTWELLGGPSLRSAYITSLYFALSSLTSVGFGNVSANTDSEKIFSICTMLVGGVLIPSRTKAASLLSQGKGNFLTPLLCLPFRQLLHSLPDELRADIALHLHKELLQLPIFEGASRGCRRSLSLTVRTSFCTPGEYLIRQGDALQALYLICSGSMEVLKDGTVLAILGKGDLIGCHISSKEPGVQANADVRGLTYCDLQCLSLPGLLASLELYPEFASKFLRELPCELSYNLGRGSVFQVSGAGRSSGAHSHLPSRSGMPRHVASETSSRPTALRLHSVPCSGPPDLSPRVVDGIEDGSNSDKPMFNFRLGPSGPDYSSPSPGTDSGLLTIPPELSGADTIEKLRQAVAELSGQVQQMREGLYSLRQAVQLFLLSQTSAQQRVDPSACGSFPHPDAPATPLLSPLRVDTGLSPAFCSHSAPPAPCPNGACGKVQWMWTPPAGQNSPWPGTFWTLGCQARDSEAGVLSPTGIGPKDPGNNTPMPNLSQRESSLPQACSTTDPAYHRSSPETKPSRTQDCDSSGLEMVLIGAGDDGSTCSGPCWIEEEGTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKN4</accession>
    <name>A0A6I9YKN4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106551111</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924599.1">
        <property type="nucleotide sequence ID" value="XM_014069124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKN4"/>
    <dbReference type="GeneID" id="106551111"/>
    <dbReference type="KEGG" id="tsr:106551111"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000024">
        <property type="entry name" value="Vomeronasal 2, receptor 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="496"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="12">
        <location>
            <begin position="388"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924599.1"/>
        </source>
    </evidence>
    <sequence length="603" mass="68472" checksum="4E015E07A1785E92" modified="2020-10-07" version="1">MTLGFHIVDSYNDEKTFYHSVLQLLFKSQQFALNYNCNSKKNVIAMIAGLNSDISFQVANILTLYKVPQLAYGSFASERREGSNPSFFRMALGEDHQVQGIFYLLRNFGWTWIGLFAQDSDGAEHFLFSLEALFPQNGICSAFTQIIPQQHLENINSWNKLAELVEKLKLHFTESKASTFIIYGDTSTLLYVASLLVQVYDECNEKACGGKVWIMTDQVDFSEELIDNIHMFQGALSFMLHSSEIKAFRAFLHLLRTKVNNLEDFWNQDLKCLFSTSDQVGKKKCTREESFQSIPTSHFEMTMNGHSYSIYNGVHAVAHALQSVGSARANSRTTRERKRLQPWQVAPHSVCNEHCQPGYQKKKKEGKKFCCYDCASCPEEKISDKKGFLCPLLFLGQPMRVTCLLRQPTFGMTFSMAISCVLAKTVTGVVAFMATRPGSNMRKWVGKRLPNSIVLCCFLIQAILCVGWMTIFPPFPDLDKYSFTKRIIAECNEEPIVIFFLVLGYMGLLSIISFMVAFLARNLPESFNEAKFITFSMLVFCNIWLAFLPAYLSTKGKEKLAVEIFSILASSAGLLSCIFVPKCYIILFKPELNSKEQLTKRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YL33</accession>
    <name>A0A6I9YL33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Fumarylacetoacetase</fullName>
            <ecNumber evidence="4 15">3.7.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Fumarylacetoacetate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106551372</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>4-fumarylacetoacetate + H2O = acetoacetate + fumarate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10244"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18034"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="3.7.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.2" evidence="4 15"/>
    <dbReference type="RefSeq" id="XP_013924947.1">
        <property type="nucleotide sequence ID" value="XM_014069472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YL33"/>
    <dbReference type="GeneID" id="106551372"/>
    <dbReference type="KEGG" id="tsr:106551372"/>
    <dbReference type="OrthoDB" id="9971669at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004334">
        <property type="term" value="F:fumarylacetoacetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902000">
        <property type="term" value="P:homogentisate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.230">
        <property type="entry name" value="Fumarylacetoacetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005959">
        <property type="entry name" value="Fumarylacetoacetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015377">
        <property type="entry name" value="Fumarylacetoacetase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036462">
        <property type="entry name" value="Fumarylacetoacetase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43069">
        <property type="entry name" value="FUMARYLACETOACETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43069:SF2">
        <property type="entry name" value="FUMARYLACETOACETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09298">
        <property type="entry name" value="FAA_hydrolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63433">
        <property type="entry name" value="Fumarylacetoacetate hydrolase, FAH, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Fumarylacetoacetase N-terminal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="16">
        <location>
            <begin position="55"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605959-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605959-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605959-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF09298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013924947.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="18373" checksum="13BFDC447A5CED04" modified="2020-10-07" version="1">MALGWEAWTEARSWLQKILSDKEPTLRDNAELRKRAFISQASAIMHLPAEIGDYTDFYSSRQHATNVGVMFRGKENALMPNWLHLPVGYHGRASSVVISGTPIKRPVGQMCPNESKSPLVAASKRLDIELEMAFFVGPGNMLGVPIPIGEAHKHIFGMVLMNDWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLD3</accession>
    <name>A0A6I9YLD3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Ribosome biogenesis protein BMS1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106551371</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924946.1">
        <property type="nucleotide sequence ID" value="XM_014069471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLD3"/>
    <dbReference type="GeneID" id="106551371"/>
    <dbReference type="KEGG" id="tsr:106551371"/>
    <dbReference type="OrthoDB" id="10260897at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="106"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924946.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="52238" checksum="427AB8CDDE17E144" modified="2020-10-07" version="1">MDLTNVQKFKGFHFKTKTGIDQHQSQISNFGKINHRKKHSGPKAGKKKKRHLKDLGIEDEEDARKRNPKAFAVQSAARMARSFHRTQDLKTKKHHIPVVDRTPLEPPPVVVVVVGPPKVGKSTLIRCLIKNFTRQKLVEIQGPVTIVSGKKRRLTIIECGCDINAMIDLAKVADLALMLIDASFGFEMETFEFLNICQVHGFPKIMGVLTHLDSFKNNKQLKKTKKRLKHRFWTEVYQGAKLFYLSGMVHGDYQKQEIHNLGRFISVMKFRPLIWQTTHPYVLVDRMEDLTNPEAIRVNPKCDRKVSLYGYLRGAHLKNKSQIHMAGVGDFTISDVSFLPDPCALPEHQKKRALNEKEKLIYAPLSGLGGLVYDKDAVYIDLGGSHSHRGREEQERPNHELVQTLILASMVECAEMRVWTSSWFGLSCSSLPRWEWLPPRSIYTASLSYTKPPSPESGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLD4</accession>
    <name>A0A6I9YLD4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Cyclic nucleotide-gated olfactory channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CNGA2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925193.1">
        <property type="nucleotide sequence ID" value="XM_014069718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLD4"/>
    <dbReference type="GeneID" id="106551589"/>
    <dbReference type="KEGG" id="tsr:106551589"/>
    <dbReference type="CTD" id="1260"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000002">
        <property type="entry name" value="Cyclic nucleotide gated channel alpha 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000030">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.300:FF:000002">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032406">
        <property type="entry name" value="CLZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF3">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED OLFACTORY CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16526">
        <property type="entry name" value="CLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="3" id="KW-0140">cGMP</keyword>
    <keyword evidence="10" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0552">Olfaction</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="380"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="528"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013925193.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="68270" checksum="1503972DA1A05518" modified="2020-10-07" version="1">MVVRGWAKKGKKEEERVDSFLARFRGPDVKGLVTDSATAGGDGLAGQRHKKKKWKVLVVDPAGNWYYRWLLVIAIPVLYNWCVLVARACFNELQRKYLLCWLLLDYLADGIYIGDIVIRMNTGFLEQGLLVKDFKKLRAKYFATLQFKLDILSILPTDLVYFAVGIHRPELRFNRLLHFPRMFEFFDRTETRTSYPNIFRICNLVMYILVIIHWNACIYYAISKAIGFGEDTWVYPNISDPEYGYLTREYIYCLYWSTLTLTTIGETPPPVRDEEYLFVIFDFLIGVLIFATIVGNVGSMISNMNANRAEFQAKIDAVKHYMHFRKVSREMEAKVIRWFDYLWTNKKTVDEWEVLKNLPDKLRAEIAINVHLETLKKVRIFQDCEAGLLVELVLKLRPQVFSPGDYICRKGDIGKEMYIIKEGKLAVVADDGITQFALLTAGCCFGEISILNIKGSKMGNRRTANIRSIGYSDLFCLSKEDLMEAVTEYPDAKRVLEERGREILTKEGLLDEAAAAESIETMDVGQKLDRLETSLETLNTRFSRLLAEYDAAQMKLKQRLTSLEAKLKEDKEEELFLEGIISPLAGEEEKTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLP1</accession>
    <name>A0A6I9YLP1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DET1- and DDB1-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DDA1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924700.1">
        <property type="nucleotide sequence ID" value="XM_014069225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLP1"/>
    <dbReference type="GeneID" id="106551185"/>
    <dbReference type="KEGG" id="tsr:106551185"/>
    <dbReference type="CTD" id="79016"/>
    <dbReference type="OrthoDB" id="8598182at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033575">
        <property type="entry name" value="DDA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018276">
        <property type="entry name" value="DDA1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879:SF2">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10172">
        <property type="entry name" value="DDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DET1- and DDB1-associated protein 1" evidence="5">
        <location>
            <begin position="3"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013924700.1"/>
        </source>
    </evidence>
    <sequence length="102" mass="11853" checksum="64B13C254BDB8FB7" modified="2020-10-07" version="1">MADFLKGLPVYNKSNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKNAAKKRDPEQVEIEGENSAPPRKIARTDSQDMNEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMA9</accession>
    <name>A0A6I9YMA9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Tumor protein p53-inducible nuclear protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">TP53INP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
            <location evidence="10">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925367.1">
        <property type="nucleotide sequence ID" value="XM_014069892.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925368.1">
        <property type="nucleotide sequence ID" value="XM_014069893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMA9"/>
    <dbReference type="GeneID" id="106551745"/>
    <dbReference type="KEGG" id="tsr:106551745"/>
    <dbReference type="CTD" id="94241"/>
    <dbReference type="OrthoDB" id="10041339at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029431">
        <property type="entry name" value="TP53INP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671">
        <property type="entry name" value="DIABETES AND OBESITY REGULATED, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671:SF0">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14839">
        <property type="entry name" value="DOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="153"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013925367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925368.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="30195" checksum="E669812700884605" modified="2020-10-07" version="1">MFQRLNNMFVGELSDLPSQEPEFSEKEEEEEWILVDFIDSCSNFSTVDEDEEEEEVDDICKPPPDDHSPIFSCLPDSLDCLADASESCFIQLDSCSMEESWFITPPPCFTAGGLTTLKVETSPLENLLIEHPSMSVYAIHNTCHNLNKNNCDEKEEQEEEEEEEEEVVESHDESNHRSEVQNKMDQHVQCYIAALAAHSTFLEQSTKSFRPSQWIKEFSERQSLNRNSLRRQNLTRDPYTRQLKSSGFFVHQPCQRQYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMQ9</accession>
    <name>A0A6I9YMQ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane 4 L6 family member 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TM4SF18</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the L6 tetraspanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925522.1">
        <property type="nucleotide sequence ID" value="XM_014070047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMQ9"/>
    <dbReference type="GeneID" id="106551883"/>
    <dbReference type="KEGG" id="tsr:106551883"/>
    <dbReference type="CTD" id="116441"/>
    <dbReference type="OrthoDB" id="8697884at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008661">
        <property type="entry name" value="L6_membrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198:SF14">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05805">
        <property type="entry name" value="L6_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013925522.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22349" checksum="E7092D5822CB883F" modified="2020-10-07" version="1">MGLQKCSGCLSCLLIPLALWSIVINILLYFPDGKSSYASSNNHTNYVWHFEGICFSGIMILILAALLVILDRDTFFECFQSESCTRTYRRFISVVLSLLGIAFSGYSLIISALSLVQGPYCKTSAGWEYAFKSTAGSYLMDPTSWSKCTEPAYVVDWNIILLSILIVLSGLQILICLLKIIAELRQILCGNYPIFVQPALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP29</accession>
    <name>A0A6I9YP29_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cullin-9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106551832</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925465.1">
        <property type="nucleotide sequence ID" value="XM_014069990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP29"/>
    <dbReference type="GeneID" id="106551832"/>
    <dbReference type="KEGG" id="tsr:106551832"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20347">
        <property type="entry name" value="BRcat_RBR_CUL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20359">
        <property type="entry name" value="Rcat_RBR_CUL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047561">
        <property type="entry name" value="BRcat_RBR_CUL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047560">
        <property type="entry name" value="Rcat_RBR_CUL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771:SF4">
        <property type="entry name" value="CULLIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771">
        <property type="entry name" value="CULLIN AND GALACTOSE-BINDING DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="117"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="348"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013925465.1"/>
        </source>
    </evidence>
    <sequence length="406" mass="47060" checksum="92658CEF219540C9" modified="2020-10-07" version="1">HKYLLDIFNKNSKTNIHAFLIQYEKALLRGYVECCSNLTWCTNPQGCDQILCKEGLCYGEACSKCSWISCFSCNFPEAHYPASCSHMSQWMDDGGFYEGMTVEAQSKHLAKLISKRCPSCQAQIEKNEGCLHMTCAKCNHGFCWRCLKPWKPTHKDYYNCSAMVSKAARQEKRFQDYNERCIFHHKAKEFAMNLRSRVSTVSDVPQIRTLTFVVDACKVLEQARKVLAYSCIYSYYNQDTEKMDIMEQQSENLELHTNALQILLEEMLLHCQDLASSIRLLKAKHFSIGLELVRRIQERLLSILHHATQDFRVGFQTRQSSEQGGIKLSNVLNGTSAYKQLIFYEGSDSPDTDDGGKEEDEEDEYVPEWQEYDEDLDDDDDISYDDESENLERDCFFFDDEDEPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR88</accession>
    <name>A0A6I9YR88_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Guanine nucleotide-binding protein-like 3-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GNL3L</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926666.1">
        <property type="nucleotide sequence ID" value="XM_014071191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR88"/>
    <dbReference type="GeneID" id="106552816"/>
    <dbReference type="KEGG" id="tsr:106552816"/>
    <dbReference type="CTD" id="54552"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000002">
        <property type="entry name" value="Guanine nucleotide-binding protein-like 3 (nucleolar)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000493">
        <property type="entry name" value="Guanine nucleotide-binding protein-like 3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF33">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="7">
        <location>
            <begin position="113"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013926666.1"/>
        </source>
    </evidence>
    <sequence length="554" mass="61792" checksum="6F7E2F981D06CCD5" modified="2020-10-07" version="1">MTRHKQKKSSKKSKHSKKKDAKSHRKKDSGVPQLSRFKEHVKKELALKQKRVKEMQERQQMARQKEIGRFRSLEGLQKDALHRQKCFEQKEMDLKQLAKHSLLEKDSSCKAYYKEFRKVIEAADVILEVLDARDPQGCRCPQVEEAVVQAGTNKRLVLVLNKIDLVSKEIVAKWLKYLRNEFPTVAFKSSTQQQSKNLQRSHVPVARASSDLLGSAACVGADCLMKVLANYCRNQDVRTTITVGVVGFPNVGKSSLINSLKRTRACSTGATPGITKSLQEVQLDKHIKLLDCPGIVMASPTSDAAMILRNCIRIEQLADPVSPVEAILKRCNKQQIMQHFSVPDFQNTMEFLAYLAKRQGKLKKGGMPDHEKAAKTVLNDWTSGKISYFTHPPETHTLPTHISAEIVTEMGKAFDFEALEQENEKALANLISGPVGISLEGSGLTSGAEIEMAEEEGLHITEEEGTKMEEDKDDELGPMTIELKSKNKTLIEAAKAVPRAPNLGEISALDPLYQGQGLRAASKKRRKRQKRADKIATKLSASLTAAMNFSLKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRV1</accession>
    <name>A0A6I9YRV1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit</fullName>
            <ecNumber evidence="5">2.4.1.255</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">O-GlcNAc transferase subunit p110</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">O-linked N-acetylglucosamine transferase 110 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">OGT</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013926340.1">
        <property type="nucleotide sequence ID" value="XM_014070865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRV1"/>
    <dbReference type="GeneID" id="106552555"/>
    <dbReference type="KEGG" id="tsr:106552555"/>
    <dbReference type="CTD" id="8473"/>
    <dbReference type="OrthoDB" id="9991317at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000013">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000019">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.150:FF:000001">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11380:FF:000001">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000012">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037919">
        <property type="entry name" value="OGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029489">
        <property type="entry name" value="OGT/SEC/SPY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13844">
        <property type="entry name" value="Glyco_transf_41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 25" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="17" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 22" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="123"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="191"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="225"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="328"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="362"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="396"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="430"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="23">
        <location>
            <begin position="477"/>
            <end position="1016"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013926340.1"/>
        </source>
    </evidence>
    <sequence length="1046" mass="116747" checksum="DD13C2BD13622CE8" modified="2020-10-07" version="1">MAASVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYKRAIELQPHFPDAYCNLANALKEKGSQVAEAEECYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVTIVADQLEKNRLPSVHPHHSMLYPLSHNFRKAIAERHGNLCLDKINVLHKPPYEHPKELKASEGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPEKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQVPCNGKAADRIHQDGIHILINMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDKETSPIEVAEQYSEKMAYMPNTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADSSAALSMPVIPMNTIAEAVIDMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDSVVYCNFNQLYKIDPATLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQSRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKSRQEYEDIAVKLGTDLEYLKKIRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHCAAGNKPEHIVNSLESGESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRW5</accession>
    <name>A0A6I9YRW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RUS1 family protein C16orf58 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106552836</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RUS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926689.1">
        <property type="nucleotide sequence ID" value="XM_014071214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRW5"/>
    <dbReference type="GeneID" id="106552836"/>
    <dbReference type="KEGG" id="tsr:106552836"/>
    <dbReference type="CTD" id="64755"/>
    <dbReference type="OrthoDB" id="364779at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF31">
        <property type="entry name" value="RUS FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="7">
        <location>
            <begin position="43"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="8">
        <location>
            <begin position="281"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926689.1"/>
        </source>
    </evidence>
    <sequence length="439" mass="48044" checksum="D35E11FB474B22A1" modified="2020-10-07" version="1">MSDMEIQTLCTESYGSRSARRYQALPNGKLSSVADASEAWHFRSLHHIFMSIFLPQGYPESVSTDYLAYQFWDTIQAFASSITGTLATQAVLKGVGVGDETSTVAAATVTWILKDGTGMLGRIAFAWSKGSKLDCDAKQWRLFADVLNDVAIFMEIVAPAFPACFTLIVCTSGFFKCIVGVAGGATRAALTMHQARRDNMADVSAKDGSQETLVNLAGLVFSLFLIPLVADNLSLTYAFYALFTVLHLYANYQAVRAVCMETLNRARLHLVLQHYLKREELPSPAVINPQEPLLLGFRQQLKVTLGAPLHTVTSSVADFQKALEGNTSNYLIFFNQPAGVISIILHRQADSVDVIKAYTHALLLEALLHQDVETCTLERGSLLSLQHQLCKGDDLGSECGGRNELGLGIGGESGGLIIAWWDWKLTPQVPFLACLIRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTB6</accession>
    <name>A0A6I9YTB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Spermine synthase</fullName>
            <ecNumber evidence="9">2.5.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Spermidine aminopropyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SMS</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = spermine + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:45725"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="EC" id="2.5.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19974"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Dimerization is mediated through the N-terminal domain and seems to be required for activity as deletion of the N-terminal domain causes complete loss of activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.22" evidence="9"/>
    <dbReference type="RefSeq" id="XP_013927301.1">
        <property type="nucleotide sequence ID" value="XM_014071826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTB6"/>
    <dbReference type="GeneID" id="106553354"/>
    <dbReference type="KEGG" id="tsr:106553354"/>
    <dbReference type="CTD" id="6611"/>
    <dbReference type="OrthoDB" id="5953636at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016768">
        <property type="term" value="F:spermine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000005">
        <property type="entry name" value="Spermine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.110:FF:000002">
        <property type="entry name" value="spermine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000059">
        <property type="entry name" value="spermine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015576">
        <property type="entry name" value="Spermine_synthase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040900">
        <property type="entry name" value="SpmSyn_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46315">
        <property type="entry name" value="SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46315:SF1">
        <property type="entry name" value="SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17950">
        <property type="entry name" value="SpmSyn_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 12" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="13">
        <location>
            <begin position="121"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927301.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="43950" checksum="798F5D434C07187E" modified="2020-10-07" version="1">MAAARHSTLDFMLSATADCNAIFKSLQSIFQKEGMTETIHNWENHGYLATYVNKNGCFANLRIYLHGLVLVDVQSCNDLNEREETEQLLNKVEEKMKELFHKSIKRIKRLPVIMRGDAIDRYWPTADGRLVEYDIDEVVYDEESVYQNIKILHSKQFGNILILSGDVNLAESDLAYTKAIMGSGKEDYTNKEVLILGGGDGGILYEIVKLKPKMVTMVEIDQMVIDGCRKYMRKTCGDVLDNIKGECYQLWGCNPGLGWLDETTRQVLIEDCIPVLKRYAKEERMFDYVINDLTAIPISTSPEEDSTWEFLRMILDLSMRVLKPDGKYFTQGNCVNLTEALSLYEEQLGRLYCPVEFSKEVVCVPSYMELWVFYTIWKKTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTG2</accession>
    <name>A0A6I9YTG2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nicotinamide N-methyltransferase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106553046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926945.1">
        <property type="nucleotide sequence ID" value="XM_014071470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTG2"/>
    <dbReference type="GeneID" id="106553046"/>
    <dbReference type="KEGG" id="tsr:106553046"/>
    <dbReference type="OrthoDB" id="10050085at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000065">
        <property type="entry name" value="Phenylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000940">
        <property type="entry name" value="NNMT_TEMT_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053384">
        <property type="entry name" value="SAM-dep_methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041360">
        <property type="entry name" value="GntF_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10867:SF32">
        <property type="entry name" value="NICOTINAMIDE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10867">
        <property type="entry name" value="NNMT/PNMT/TEMT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01234">
        <property type="entry name" value="NNMT_PNMT_TEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000384">
        <property type="entry name" value="PNMTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51681">
        <property type="entry name" value="SAM_MT_NNMT_PNMT_TEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="59"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013926945.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29121" checksum="0BE23395F579AE74" modified="2020-10-07" version="1">MEFTGGDVYQKSFDPKVYLEYFHFGKGSVEDEFLEFILKCFCKIFSSGVLKGGTLIDIGSGPTIYQLIPACGSFKNIIATDYLDRNCQEITKWLKKEPGAFDWSPVVQYVCQLEGNRETCAEKEAKLRRSIKQVLRCDVHQSNPLAPISLPQADCLLSTECLESACNDQPSFHAALKNLSSLLKLGGHLVLGGVLGCTFYRVGPQRFSSMLFTEEMLKNGLSAAGFAVVEFNVEPRVFKTNMEDCDFSGKYVILARKDKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX19</accession>
    <name>A0A6I9YX19_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Growth factor receptor-bound protein 14-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106554974</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GRB7/10/14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929203.1">
        <property type="nucleotide sequence ID" value="XM_014073728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX19"/>
    <dbReference type="GeneID" id="106554974"/>
    <dbReference type="KEGG" id="tsr:106554974"/>
    <dbReference type="OrthoDB" id="5990423at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01259">
        <property type="entry name" value="PH_APBB1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000062">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000133">
        <property type="entry name" value="growth factor receptor-bound protein 14 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039665">
        <property type="entry name" value="PH_APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF22">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Ras-associating" evidence="7">
        <location>
            <begin position="185"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="313"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="433"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013929203.1"/>
        </source>
    </evidence>
    <sequence length="510" mass="58651" checksum="7DFCB03813F73C9E" modified="2020-10-07" version="1">MEQIQDINKLVVVAQFSADVTLNTMKFASRAMPLSVVSHRLLWLRGWQADNKAKWQLATAAYMGGSLLGISLDAILVEDKNKKKALPTSTKKVEQRSTPYYRRDIHGCLGPQYGDPCLHLEFLSLLPNCFRVFPPAKNVDKCQQMRQAIQHLLDIAAIERVPEDQAAPPAKSISSVQQSLHIGIAPRVIKVYSEDETSRAFEIPSDITSRDLCQLLILKNHYVDDHSWTLFERLTYLGLERIVEDHEMIVEVHSKWGIEEDNKFYFRKNYAKYEFFKAPEIFFPDHLVSLSNESNGTTNHAQILQMFLSSTAYPEIHGYLHFKEQGKKTWKKMYFLLRRSGLYFSTKGTSKEPRHLQLFSEFSCSDIYMSLRGKKISGVPATFGFCFKPYKAGGTRDLKYLYADSEQSRTCWMTAIRLLKDFCITIVIGKLVFLVRDSQSNPKTFVLSLSHGLKIKHFQIVPLEEEGKLFYTLDDGHTRFSDLIQLVEFYQLNKGILPCKLKHYCARIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXF7</accession>
    <name>A0A6I9YXF7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 32-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106554542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928719.1">
        <property type="nucleotide sequence ID" value="XM_014073244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXF7"/>
    <dbReference type="GeneID" id="106554542"/>
    <dbReference type="KEGG" id="tsr:106554542"/>
    <dbReference type="OrthoDB" id="8195690at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000226">
        <property type="entry name" value="leucine-rich repeat-containing protein 32 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="646"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928719.1"/>
        </source>
    </evidence>
    <sequence length="680" mass="77744" checksum="95F441AC90E286ED" modified="2020-10-07" version="1">MHLVMLCQSDVIVHLIILCPLCRYNAIQAFYTKMIHFLLLLQQRNLEALCRNVDLHQVPPDLPPYVSKIDLSGNKIRNVTESPLTFFASLRHLDLSSNLIGFVKPGIFAEMKNLEDINLANNRLYLLAQEDLWVGLLPHVRRLDLSRNSLYSGMAEHFIHQAPSLRYLSLAENSIIHLSHSMFLGSPELVEVNLHNNMIMEIEEGVFESLTRLSKLDLSMNSLTCIVEFNLRQLEFLDLSRNSLESFHSTQSEEDFNLIWLDLSQNKLLRFPVLPQGKKLTYLNLTNNIVQFVMVDSRNDVDYQWEDGPQYPNNSHPPYLPALLSLDLSYNEIQSIPSQFFDSMLSLQFLNLSKNCLQTFGVTNELASLSVLDLSCNSLKDLLLDTNALSGLQELHLQDNHLQDLRPDIFQGLPHLQRLDLRHNKVHLCHTHSVAGRHRLSPNVPRGCVSFVRVPELQYLYLSANRLKDLPMYNFFGTKLRVLDVSMNWGLQIGAQSLVGLEFSLEYLDLHGNGLTTLNVDFSLFPHLRYLNLSDNRLSWLPAWSEGCCVLEVLDLHNNSFNSLKNSQIPALEKNLRNLYLVGNPLSCCGNVWLSQMIHKAVVEIPDLDLVKCQFARSLGFEEEEVNVSHVRPEDCEREDLKKTSILILLATLLVLSLIVVGVGLLCCYRRNLFGRHYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY05</accession>
    <name>A0A6I9YY05_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Delta-like protein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106554718</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928924.1">
        <property type="nucleotide sequence ID" value="XM_014073449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY05"/>
    <dbReference type="GeneID" id="106554718"/>
    <dbReference type="KEGG" id="tsr:106554718"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000255">
        <property type="entry name" value="Sushi, nidogen and EGF-like domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000472">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF9">
        <property type="entry name" value="NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="47"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="85"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013928924.1"/>
        </source>
    </evidence>
    <sequence length="275" mass="29779" checksum="F79204869E413E20" modified="2020-10-07" version="1">MDVHLKGISSDRLFPTFATRGGRCLDLGRSAFCKCRPGFAGSRCELNIDDCACNPCANGGTCIDGPNSYSCSCTLGYGGKDCSVRMDACSSSPCQNSGTCYTHFSGHVCECPSGFMGSNCEFRVQLPTPVSSPWMAEGPFPTALAVSFALGLLTLLLAACAILALLRHMRNGPHPLKSRVCNDLAAVNNFRERESRLLPRGCFKVSNKDGRFDGDSFNCKRKLLDQSYESICKKPDNKPSELSWPVVAECPKDGAYHPIYIIPGQVEPCVYATEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYP8</accession>
    <name>A0A6I9YYP8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Perilipin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106554943</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929169.1">
        <property type="nucleotide sequence ID" value="XM_014073694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYP8"/>
    <dbReference type="GeneID" id="106554943"/>
    <dbReference type="KEGG" id="tsr:106554943"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF11">
        <property type="entry name" value="PERILIPIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929169.1"/>
        </source>
    </evidence>
    <sequence length="88" mass="9300" checksum="394F2D4853956408" modified="2020-10-07" version="1">MSSEEKAENHLVPDGQALEQQNVISRVAGLTLVSSTCDMVSSAYTSTKETSPYLKSVCDVAEKGVKTLTAAALCGAQPLVTRLEPQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0E5</accession>
    <name>A0A6I9Z0E5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 58</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CFAP58</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929330.1">
        <property type="nucleotide sequence ID" value="XM_014073855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0E5"/>
    <dbReference type="GeneID" id="106555087"/>
    <dbReference type="KEGG" id="tsr:106555087"/>
    <dbReference type="CTD" id="159686"/>
    <dbReference type="OrthoDB" id="264785at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049270">
        <property type="entry name" value="CFAP58_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083:SF31">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21771">
        <property type="entry name" value="CFAP58_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 58 central coiled coil" evidence="4">
        <location>
            <begin position="366"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="853"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="106"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="645"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="784"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013929330.1"/>
        </source>
    </evidence>
    <sequence length="876" mass="103684" checksum="67D83D39007CFB56" modified="2020-10-07" version="1">MAEEKYDKPIIEENVFEALEKDFQEVISELTGDQSLEKFRVEYEKLHAVLKKSYENEKRLMAKCRELNAEIVVNSAKVATALKLSQDDQTTITSLKREIEKAWKMVDAAYEKEQKAKETIMSLKEEILNLTKLVEQGSGLSLGQEHNLRELLKFKEEVTKERDQLLAEVVKLRDSVANMADQQQEAEKTKLEAESTIAQLHQEIQMRQNEASRETRKKEKMERELKQLQNDLEFKMADIKTMQQGLNKSKEELLKCEQQLKEQKILNERATKELEQSTARNTKLQLENEQHSLAIEQLMQENQQKTTELRAREDEVSQMRQEIAKLTKMREVMQRKLRVVEENKTQAECERDTLKNQITGLEKELEAAKKQAEVDKKAIDELVRERDILNKNLLKAASVTQKQMNLVKLHEQSKKNLEEEIQNYKDEAQKQRKIIFQLEKERDRYINEASDLTQKVLHHMEDIKLREMQIFDYKKKIAESETKLKQQQNLYEAVRSDRNLYSKNLIEAQDEINEMRKKLKIMTHQVDQLKEEISVKEAALVKVHLDHQRIEKEKEALKGELLKMRQQALETKHFIEKQEEEERKLLKIISEADAERLRQKKELDQVISERDILGSQLVRRNDELALLYEKIKIQQSILNKGEIQYRQRIEDIRLLKLEIKKLRREKAILGKSVANVEELRQEVYHMQKELLKERTRCRALEEELENPMNVHRWRKLEASDPSTYELIQKIHALQKRLISKTEEVVEKELLLQEKEKLYVELKHILARQPGPEAAEQLQLYRHTLREKTKQLKVLSSELNMYESQSQEYKYEIERLANELLNIKKKYLTQKRKEQENREKEKALSNIEHDFSGQRARVPRFTGGGFPLSNPPPKIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0S4</accession>
    <name>A0A6I9Z0S4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Wilms tumor protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">WT1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929899.1">
        <property type="nucleotide sequence ID" value="XM_014074424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0S4"/>
    <dbReference type="GeneID" id="106555553"/>
    <dbReference type="KEGG" id="tsr:106555553"/>
    <dbReference type="CTD" id="7490"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072078">
        <property type="term" value="P:nephron tubule morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000228">
        <property type="entry name" value="Wilms tumor 1-KTS isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000241">
        <property type="entry name" value="Wilms tumor 1-KTS isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000976">
        <property type="entry name" value="Wilms_tumour_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF49">
        <property type="entry name" value="WILMS TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02165">
        <property type="entry name" value="WT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00049">
        <property type="entry name" value="WILMSTUMOUR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="325"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="386"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013929899.1"/>
        </source>
    </evidence>
    <sequence length="421" mass="47000" checksum="E728102388315D4A" modified="2020-10-07" version="1">MGSDVRDLNALLPPSVPPLPGNGNCSMPVSSATQWAPVLDFPPAASYGSLAPPPSAFIKQEPSWSSGEPHEDQCLSAFTVHFSGQFTGTAGACRYGPFAPPPPPSQAPSGQARMFPNNPYLPNCLESQQAIRNQGYGTVAFDGAPSYGHTPSHPGAQFTNHSFKHEDPIGQQTPLGDQQYSVPPPVYGCHTPTDSCSSSQALLLRNPYNSDNIYQMTSQLECMTWNQMNLGSTLKGHGTGYESDNHAAPMLYSCGAQYRIHTHGVFRGIQDMRRMSGVAPTIVRSASDTNEKRPFMCVYPGCNKRYFKLSHLQMHGRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHNRNMTKLQLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1D4</accession>
    <name>A0A6I9Z1D4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106555735</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013930104.1">
        <property type="nucleotide sequence ID" value="XM_014074629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1D4"/>
    <dbReference type="GeneID" id="106555735"/>
    <dbReference type="KEGG" id="tsr:106555735"/>
    <dbReference type="OrthoDB" id="10070999at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd05609">
        <property type="entry name" value="STKc_MAST"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Microtubule-associated serine/threonine kinase 1a"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.510.10:FF:000012">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 2 isoform X1"/>
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    <dbReference type="FunFam" id="1.20.1480.20:FF:000001">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1480.20">
        <property type="entry name" value="MAST3 pre-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR037711">
        <property type="entry name" value="MAST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015022">
        <property type="entry name" value="MAST_pre-PK_dom"/>
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        <property type="entry name" value="MAST_pre-PK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF224">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED SERINE_THREONINE-PROTEIN KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08926">
        <property type="entry name" value="DUF1908"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="444"/>
            <end position="717"/>
        </location>
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    <feature type="domain" description="AGC-kinase C-terminal" evidence="16">
        <location>
            <begin position="718"/>
            <end position="792"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="38"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="918"/>
            <end position="954"/>
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            <begin position="1"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="123"/>
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        </location>
    </feature>
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        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
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        <location>
            <begin position="150"/>
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        </location>
    </feature>
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        <source>
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            <dbReference type="PROSITE" id="PS50011"/>
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            <dbReference type="PROSITE" id="PS51285"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_013930104.1"/>
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    </evidence>
    <sequence length="978" mass="110026" checksum="7A62B5A63AE68D3E" modified="2020-10-07" version="1">MPFRKSSNPEVSSGPGKSLKFKRQLSEDGRPFRRGSLGGALTGRYLLPNAVTQHSWQTGETSNLVRMRTQALGQSAPSLTASLKELSLPRRGSFCRTSNRKSLICNGQSSAMPRPHSPLSAHAGSSPQDSPRNFSPSASAHFSFARSHGHRNERTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCATESIASDNRCRTTQMRPRSRSLSPGRPPACCDHEIIMMNHVYKERFPKATAQMEERLQEIITNHSPENILPLADGVLSFTHHQIIELGRDCLDKSHQGLITSRYFFELQHKLDKLLQEAQERSESGVLAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEIAQLTNYDSGAAATPETDEPNSNASLKLKRKPRENDFETIKLISNGAYGAVYLVRHKETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETKRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWSMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLIALLLRQNPLERLGTGGTYEVKQHQFFQNLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEEEEDTNDEDFNLEIRQFSSCSHRFSKVFSNIDRVTIQTEEKENIVEGTTNATLTSSESSCWSSEYSDMQQISTSVSSDTESVKQRHSSGLLPKLAISAEGDSEELLCSVGSHKLQEKTVFISEETVQDEVEVTTPASTISNSTLSAGSFSEHMDQINGRNESVDSTDNFSKPSTEQASHMARQRLETTEKKKIPGKVTKSLSASALSLMIPGGRYCLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2J8</accession>
    <name>A0A6I9Z2J8_9SAUR</name>
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        <submittedName>
            <fullName evidence="8">Ly6/PLAUR domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LYPD1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="term" value="F:acetylcholine receptor inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TFP_LU_ECD_LYPD1"/>
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    </dbReference>
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        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10036">
        <property type="entry name" value="CD59 GLYCOPROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10036:SF7">
        <property type="entry name" value="LY6_PLAUR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPAR_LY6"/>
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    </dbReference>
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        <property type="entry name" value="LU"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027025020" evidence="5">
        <location>
            <begin position="21"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="6">
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            <begin position="23"/>
            <end position="121"/>
        </location>
    </feature>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013931283.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="15235" checksum="A08977390F2D8CEF" modified="2020-10-07" version="1">MWLLAVTTTFWGLLLAPGFALQIQCYQCEEFQLNNDCSAPEFIVNCTVNVQDMCQKEVMEKSSGILYRKSCASSAACLMTSAGYQTFCTPGKINSVCISCCNTPLCNGPRPKKRGNTGTMPRAGVLTTILLSSLPFLSHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z331</accession>
    <name>A0A6I9Z331_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Copine-7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
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            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
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            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930634.1">
        <property type="nucleotide sequence ID" value="XM_014075159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z331"/>
    <dbReference type="GeneID" id="106556176"/>
    <dbReference type="KEGG" id="tsr:106556176"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000163">
        <property type="entry name" value="Copine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF6">
        <property type="entry name" value="COPINE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930634.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="22083" checksum="7AB7E8A685122B00" modified="2020-10-07" version="1">MNEVLDPAANLGVVSATTCVSKVELRICCKHLLDRDTLNKSDPCVVLLMQSQGQWAEVERSEVIRSNLNPVFAKVFTVDYYFEEVQKLRFEVYDIHGHGGPGTHDDDFLGGMECTVGQIVAQKRVSKPLFLKYGKPAGKSTITVTSEEIFGDNGFVELFFRAKKLDDKDLFSKSDPFLEIYRINDDGSEQLVHRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3R4</accession>
    <name>A0A6I9Z3R4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ectonucleoside triphosphate diphosphohydrolase 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106556060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930500.1">
        <property type="nucleotide sequence ID" value="XM_014075025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3R4"/>
    <dbReference type="GeneID" id="106556060"/>
    <dbReference type="KEGG" id="tsr:106556060"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046036">
        <property type="term" value="P:CTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006256">
        <property type="term" value="P:UDP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000057">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF29">
        <property type="entry name" value="ECTONUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="183"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013930500.1"/>
        </source>
    </evidence>
    <sequence length="209" mass="23599" checksum="8455518B5030AE2C" modified="2020-10-07" version="1">MVDCGSSGSRIFVYWWPRHNGNPHDLLDIKQMRDKNRKPVVMKIKPGISEFATSPEKVSDYIFPLLNFAAEHIPRAKHKETPLYILCTAGMRILPESQQKAILEDLLTDIPVHFDFLFSDSHAEVISGKQEGVYAWIGINFVLGKFEHMDEEDEDVVEVHVPGSESKEEVVRKRTVGILDMGGVSTQIAYEVPKTVSFASSQQEEVAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3S5</accession>
    <name>A0A6I9Z3S5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Phosphatidylinositol phosphatase PTPRQ</fullName>
            <ecNumber evidence="7">3.1.3.67</ecNumber>
            <ecNumber evidence="6">3.1.3.86</ecNumber>
            <ecNumber evidence="5">3.1.3.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">Receptor-type tyrosine-protein phosphatase Q</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">PTPRQ</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="36">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25528"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="EC" id="3.1.3.86"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:25529"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.67"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:25018"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32955"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:32956"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:39019"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="EC" id="3.1.3.95"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:39020"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="27">Interacts with TPRN. TPRN, CLIC5 and PTPQR form concentric rings at the base of stereocilia and may form a complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Basal cell membrane</location>
            <topology evidence="26">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Stereocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.67" evidence="7"/>
    <dbReference type="EC" id="3.1.3.86" evidence="6"/>
    <dbReference type="EC" id="3.1.3.95" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013931748.1">
        <property type="nucleotide sequence ID" value="XM_014076273.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106557125"/>
    <dbReference type="KEGG" id="tsr:106557125"/>
    <dbReference type="CTD" id="374462"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="CDD" id="cd14616">
        <property type="entry name" value="R-PTPc-Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000937">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001431">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000041">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001685">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001147">
        <property type="entry name" value="phosphatidylinositol phosphatase PTPRQ isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000478">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000869">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001474">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001645">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002575">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL PHOSPHATASE PTPRQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="10 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 30" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="2870"/>
            <end position="2892"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="31">
        <location>
            <begin position="202"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="423"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="782"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="871"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="1046"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="1137"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="1236"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="1382"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="1639"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="1739"/>
            <end position="1828"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="1833"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="1928"/>
            <end position="2016"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="2021"/>
            <end position="2121"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="2126"/>
            <end position="2219"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="2224"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="2316"/>
            <end position="2409"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="2410"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="2505"/>
            <end position="2603"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="34">
        <location>
            <begin position="2608"/>
            <end position="2709"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="32">
        <location>
            <begin position="2967"/>
            <end position="3223"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="33">
        <location>
            <begin position="3143"/>
            <end position="3214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00045090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00060472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00063599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00071873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00082323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="RefSeq" id="XP_013931748.1"/>
        </source>
    </evidence>
    <sequence length="3262" mass="366622" checksum="5FB8FA37D73832DE" modified="2020-10-07" version="1">MDKTMCFLIVTQYVFQITNNLKCDKVKNPSRAVSLEWSKFPENQYPDFYIITYHIPDDFAQKNMKSVPTIQVKVQKVTVLLEEGKKYDVMIQSVKSGKVLYAKSFKIYGIPESSVKTRVTSTSVLFNWNMLVHDFSIGIFHKNISYIFKNKERVYEWTDLRPATLHTFSLQFKQLDLEFINVYQILEFEIETGFCSDGWTAFKQSCYKVDTDSKPWNMAQHQCELFSTGAHLVDIRNKEEYTFLTSYLQSFNQLNLVWTGLNDIKEEGRLMWTDDSLYLGESEFSSLSLIPENETDCYAFQRNPFVPDYLFIGFFCYISLPYICEYELSSVHENFTFKIQDVSTNEATFLWNDLNGWLNSNFQLNIKYYSEHSEQNSKFFTLNSTCTKIFNLDPGHSYKFLLSAQSPKGAQISLHPVVTIETRPLHLHNVKATRVTSSEIFLQWDSPASSFNASFHHYLVNIFDSKTIKWKTFSFGKTNTSAVIRSVKSYNQYWTYVQSVAEKGTHSCYEDPIEIITGVTPPTGLLIHPEDVGEDHVIISWKLPEEGQESYIQVKPTAFKDKSMMFLVNNTEKFKIEPLIPGMTYDIGVATIISGNKSDLTTIQCTLKPKPVQMVIPYEIHSTFLILFVKVPALGIIDGIHIISKEGTNATFTLLNDGKVTIEKLTPGTEYYFFVFTFSRHMLSSVHHLPAVKTCLAVPLNIYEGRITNISIQIIWDQAEGNFQQYEVTCTNCADTYRVQKVTQEVAEFSNLIPGFLYNFMVKTEKEGYKDSPPVSIQVETEPSSVKYMNLSKDSGSITVTWPPAFGIVDGYITTLVGKNYSKEERLSSDKRKFTFDGLSSGTEYFVQIVTTNRLKNSFPTFLRVTTDPDPPLALQALRIEENTVYLFWKLPNGGLDNFELSYCLITGNKTLQTTIMSDNRAVVKNLMPGVEYIFQIKTIRGQAASIPAKIKITTKPMKICGLTLTLRNTTSAKLTWNPSETDFSHYKASVSNKTFTKEYTILGPETDHIITNLTPGSIYNFTLQRVKGSVESAVSFTEFVTEPEKPQKLTVFNVSSHSFSLHWRLPNGCVEQFYVQLAPPHGFVSLQDMGSGEYQAEILNTTPGTIYNVTVSSVSSSVYSLPVSKTVTTNVTNPGPPVFLAGEKVGSAGILLSWNTPHFPNGRIISYIVKYKEVCPWMQNIYTQVTTKPDSLEVLLTNLNPGTTYEIQVAAENSAGIGIFSVPFLFQTAESAPGKVVNLTVEAFNHSAVNLIWFLPQQPNGKITSFKISVKHARSGIIVKDVSVKVEDLLSGRLPECTDNSESFLWSTTTPSTTSSKTTTFTSSTVSPKAMSSVWNEPVSFVVTNLRPYTTYLFEVSAVTTEAGYIDSAIVRTPESVPEDPPQNLATGNVTGKSFSISWEPPNIVTGKFTYKVELYGQTVRILDNNTKDLKFTFNNLIPFTLYDVFVAAETSAGVGPKSNITVFTPAGVPGPVFDLHVVEEAASFIRIAWKKPQQPNGVITQYRVKVFHEETEEILEDTILTEKIKHFTESVDPNMTEEYALSSSPNKIKTVTSLYEGSGEMFPRVHEFSPVKYTSIPVDNFLTVNEDAGLYLNSVEQLSYRVNALQPFTKYAIAVSAFTIIGEGPPAILISKTSEQVPSSVENIDYKNISSSSILLFWDKPLNPNGKITHYTIYAMEVDTNKAFQLTTSEKHILITDLKKYTDYKMRVTASTTVGESALSGDNDIFIRTLEDEPSSPPQNVEALDVTASEVKLRWLPPEIPNGIITHYEIMSNNGSILITKNATTTHLTLNELKPYTLYNISVRAFTRLGHGNQSSDPLSVRTAEAVPDFPPQNITYRNMSSSVIELSYFPPSVPNGIIKTYTIYLREMNGSEVRVINTTHLSLIITGLKKYTKYLAEVSSSTVKGQGPRSAPLDIITDEDAPSSPPRLLTVKQLSDAIVVLSWQPPLEPNGIILYYTVYIWDIMSRNINASELSLELTDLEYNKKYSVYVTASTRFGDGNIKSDTTSFRTSEGAPGDPPKNLWYKNLTSSSVRVFWNPPQKPNGVIQFYTIYYKNDSDTYVQNFTTSTIDSSADNSTLSIVLNNLAKWKHYTLWLTANTALGNGNQSSEIMQLYTDQDLPDGPVTQLSYQNISSTVINVSWIPPMQPNGLIFYNISLTMKDTQTNDEILSLLLYDTNIIFNKLEKYANYLLNITPATEKGSSEMYTLSVYIRTEEDVPDSAPILKTYRNLSSTSVMLSWDPPYRPNGIIRGYDLKLQGPEKNISFTTSNNSIILEDLLPFTLYSFFASAQTVKGPGPYSVLMFSTDESVPLAPPENLTVINYTYNSAWLKWDPSPRPNGVIRRYVLKISENSSQATFYQNISGSHNEANIFGLEPFSVYSISVSAFTKVGNGNQFSNHVQFTTMESAPGIVQNTQCIATSWQSILLQWDPPLKTNGIITDYIITFSENSTISSSHNETVCILRNLLSNTTYQFKIKAATAAGEGKEQLCNTSTFPETAPDAPPANVTAVATSPYSINISWNEPTIITGPTFYHIDISSVDADYYQASFVKTNYEDKTLSISGLRAFTRYSIVVTAFVEDVQTAHTEGKSSSSVIVSTFEAVPRDPLNNVTFQKIPDEVTKFQVMFVPPSELNGNIQEYQAMVYKDDDIDIFQVYNLSIIDRTNKSITAMIEGLKGGHTYNISVYAINSAGAGPKIHLKITTDIKEPPRPSKKPAPVYDTSGTLLATATTITIHMPVCYYNDDHGPIKKIQILVAEAGVQNDENITKWYDAYFNKARPYLVNEGFPNPPCIKGNEGVSTKEDIYVIGADSTCMIPGSEEKICNGPLKPKNQYVFKFRATNIRGQYTDSDYSEPIKTLGDGLSERAVEIILAVTLCILSIVLLVAIIYAFARIRQKQKEGGTYSPRDAEIIDTKFKLDQLITVADLDMKDERFTRLLSYRKSIRPIGKKCFLQHVEDLCTNNNIKFQEEFLELPKFLPDLALTDADLPWNKSKNRFPNIKPYNNNRVKLMPDACVPGSDYINASYVSGYLCPNEFIATQGPLPGTVGDFWRMVWETRAKTVVMLTQCYEKGRIRCHQYWPEDNIPVTFFGDIVITKLVEDVQIDWTTRDLKIEKHGDCMMVRQCNFTAWPEHGVPETSASLVHFAKLVRGSRSHDNTPIIVHCSAGVGRTGVYIALDHLIQHINDHDFVDVYGLVAELRSERMCMVQNLAQYIFLHQCVLDLLTIKNNQPLCFVNYSVLQKMDSLDAMEGDVELEWEETTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5R9</accession>
    <name>A0A6I9Z5R9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aquaporin-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">AQP5</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Plays an important role in fluid secretion in salivary glands. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Seems to play a redundant role in water transport in the eye, lung and in sweat glands.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer; each monomer provides an independent water pore. Interacts with TRPV4; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931291.1">
        <property type="nucleotide sequence ID" value="XM_014075816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5R9"/>
    <dbReference type="GeneID" id="106556807"/>
    <dbReference type="KEGG" id="tsr:106556807"/>
    <dbReference type="CTD" id="362"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015670">
        <property type="term" value="P:carbon dioxide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000003">
        <property type="entry name" value="Lens fiber major intrinsic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023276">
        <property type="entry name" value="Aquaporin_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF38">
        <property type="entry name" value="AQUAPORIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02017">
        <property type="entry name" value="AQUAPORIN5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013931291.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="28170" checksum="FB4170D6D7F32319" modified="2020-10-07" version="1">MRKEVCTVVFLRAVFTEFLATMIFVFFGLGSALKWPSALPTVLQISLAFGLAIGTMVQTFGHISGGHINPAVTIAFFVGNQISFLRMMFYIVAQLLGAIAGAGILYGVTPPNTRGNLAANGLSNNTTAGQAVVVEIILTFQLVLCIFASTDNRRNGNVGSPSLSIGLSVTLGHLVGIYFTGCSMNPARSFGPAVVMKRFTSAHWVFWVGPISGAILASLLYNYLLLPHSMNMSERVAIIKGTYESEEEWEEKEKSMEMSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z660</accession>
    <name>A0A6I9Z660_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calglandulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MYL4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the cellular control mechanism of the secretion of toxins from the gland into the venom.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Myosin is a hexamer of 2 heavy chains and 4 light chains.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931421.1">
        <property type="nucleotide sequence ID" value="XM_014075946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z660"/>
    <dbReference type="GeneID" id="106556907"/>
    <dbReference type="KEGG" id="tsr:106556907"/>
    <dbReference type="CTD" id="4635"/>
    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000019">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000056">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF1">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="4" id="KW-0518">Myosin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="22"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013931421.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19104" checksum="ED424B9DF434A8C1" modified="2020-10-07" version="1">MSLQPRKKRRRIEEEEIEFSADQIEEFKEAFMLFDKTPTGEMKITYSQCGDVMRALGQNPTNAEVLKVLGKPKAEEMNSKMLDFETFLPMLQHIARSKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTENEVEQLMAGQEDANGCINYEAFVKHIMSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6C9</accession>
    <name>A0A6I9Z6C9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Probable ATP-dependent RNA helicase DDX56</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">DEAD box protein 56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">DDX56</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Nucleolar RNA helicase that plays a role in various biological processes including innate immunity, ribosome biogenesis or nucleolus organization. Plays an essential role in maintaining nucleolar integrity in planarian stem cells. Maintains embryonic stem cells proliferation by conventional regulation of ribosome assembly and interaction with OCT4 and POU5F1 complex. Regulates antiviral innate immunity by inhibiting the virus-triggered signaling nuclear translocation of IRF3. Mechanistically, acts by disrupting the interaction between IRF3 and importin IPO5. May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">May form homooligomeric complexes. Interacts with IRF3. Interacts with OCT4 and POU5F1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013931390.1">
        <property type="nucleotide sequence ID" value="XM_014075915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6C9"/>
    <dbReference type="GeneID" id="106556879"/>
    <dbReference type="KEGG" id="tsr:106556879"/>
    <dbReference type="CTD" id="54606"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000939">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001046">
        <property type="entry name" value="Probable ATP-dependent RNA helicase ddx56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 23" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="5"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="36"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="228"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="469"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="17">
        <location>
            <begin position="5"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013931390.1"/>
        </source>
    </evidence>
    <sequence length="554" mass="62044" checksum="886BEE4DFA1B3CDE" modified="2020-10-07" version="1">MAAEGSFGQMGLDARLLQAVAELGWAAPTPIQAKAVPLALEGKDLLAKARTGSGKTGAYALPLLQRLLDAKAAALVTEQAVRGLVLVPTKELARQVLQMLRRLAAYCARDIRTLDLAGPEDLGAQRPLLMEKPDVVIGTPSRVLAHVQMQNLRLQNSLEILVIDEADLLFSFGFEEDLKNLLCHLPKIYQTFIMSATFNEDVQTLKELVLHNPVTLRLQEAPLPDSSQLSQFQIQCETEEEKFLLLYALLKLSLVRGKVLLFVNRIDRCYRLKLFLEQFGIAACVLNSELPAQSRCHIISQFNRSVYNYIIATDEQHLAEPEESGRKRKKGTKGEKGKDQEYGVSRGIDFHNVSAVFNFDFPPSFESYIHRAGRTARVHNPGTVLTFVLPSERLLLAKIEEVLTAESTSKCTLTPYQFRMREIEGLRYRCQDAMRSVTKQAIKEARLREIKEELLNSEKLKSLRVSGQQSQPWLGNASGGNARPPLARARGTPCTASGAPRRNFGPWPRPNKPPAPASGGMSERDRNWLPFLRCQKCHEAALTHFQFFFFLRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6K7</accession>
    <name>A0A6I9Z6K7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ataxin-7-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ATXN7L2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931470.1">
        <property type="nucleotide sequence ID" value="XM_014075995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6K7"/>
    <dbReference type="GeneID" id="106556945"/>
    <dbReference type="KEGG" id="tsr:106556945"/>
    <dbReference type="CTD" id="127002"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052237">
        <property type="entry name" value="Ataxin-7-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117">
        <property type="entry name" value="ATAXIN 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117:SF5">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="2">
        <location>
            <begin position="270"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="746"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931470.1"/>
        </source>
    </evidence>
    <sequence length="782" mass="84636" checksum="5A94E75CF47CCFFF" modified="2020-10-07" version="1">MAVRGRAAVVAAVAMAAVDRRLPNLDDFAGQSWSAWVERVGPPTTETVSDLEENSRNGRKKIDAMTLIKDDMSIFGHCPAHDEFYLVVCNHCGQVVKPQAFQKHCERRHGPLGKLCGQVQPSSNLQKCHAVNGRPIVGGSHGSTKSWREKSQSFFNQTQHVPEKTDKMPKDNLCLFVPVVNLEKISSLPKSDGDGIKTPALLSDTAIPKQSPVNSKEVLGKPSLLASPKELMTSAKAGSTSIIMADTLNRKLENASSLSEKELGVVKSLKKLSRKECDLNRQCGVVNPETKKVCTRLLTCKIHSVHQRREVQGRTKDFDVLVAELKASTKKGESPKDKSPVRKDIASERLLQDSSSMSQTSLVLANVSASSSHAKQPYSHCALSRSGVSSESDPDDVPISSSEIDNRLYRSPASKSTGQVTSEESEEDATDDSRKLDCRYATRRPHPQAFCTFGSRLMSPGCYVFSRRLDRFCSALSSMLERHLSSHMWKRIPPAADHPISTSSPMLLGFSTSNTSPNYNSSLVCSLPHSTPSRTSVSWATSVTMASRDSRNHHSVNYAVGSPHAAAACSQSDCMGGSQSITSPLPANTPSPSFSKLPSNKASKSSKAKEMAGNVEPEALARKRKQSPSVPASPPYKQSCFLDLGKNKAVGCQVSHPSAKAKTSVVASSNLSLNGTVSSGARVKRVNHLDCRASSHMPVKASSLENQGCSLNGPKTLLTNCISDEEAKRRKNSATYCRPVKTKHLPSSMPSCSSSDLGGTIRRKKPVVSFEEKQNTMKSKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7D0</accession>
    <name>A0A6I9Z7D0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protease serine 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106557354</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932049.1">
        <property type="nucleotide sequence ID" value="XM_014076574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7D0"/>
    <dbReference type="GeneID" id="106557354"/>
    <dbReference type="KEGG" id="tsr:106557354"/>
    <dbReference type="OrthoDB" id="10051896at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035821">
        <property type="term" value="P:modulation of process of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF21">
        <property type="entry name" value="TRANSMEMBRANE SERINE PROTEASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013932049.1"/>
        </source>
    </evidence>
    <sequence length="287" mass="32605" checksum="818A3064CB80618C" modified="2020-10-07" version="1">SEPYMTAVSTNRGECTARVKGITSSFSATQRHCPTLFEIDFGQFSEAVSRAKASCDLEDPEMWRAVIGLHHLFKHKTHTIKKRIKEIKIFHYYNSAKYEDDIAVFKLSKPVIFNDYIQPICLPNADLALTSEMKCFISGWGTNKEKGKGTLILQEAQLQIFSLDTCNQFDWHAGSIPDTAFCAGSETGDVDTCQGDSGGPLVCYLSDSKYYIVGITSYGVGCGRPKYPGVYTNLPKYMFWVYYLFFNPQEFVESNLMNVYKTMLEKQLFHVLNYFSSEKYALSFETM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7D8</accession>
    <name>A0A6I9Z7D8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LOW QUALITY PROTEIN: DENN domain-containing protein 2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DENND2A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013932054.1">
        <property type="nucleotide sequence ID" value="XM_014076579.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106557358"/>
    <dbReference type="KEGG" id="tsr:106557358"/>
    <dbReference type="CTD" id="27147"/>
    <dbReference type="OrthoDB" id="10266080at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000004">
        <property type="entry name" value="DENN domain-containing protein 2C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF3">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="567"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="377"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="499"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="711"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="719"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013932054.1"/>
        </source>
    </evidence>
    <sequence length="1123" mass="127628" checksum="5893D594AEB66C25" modified="2020-10-07" version="1">MPAANTMTGSAVPSVESKPEIIICKNIERIRFASLNNPAAKTTFWGGLSKHNWSKSVAFPAKLLLKAELDSRAESPLKTVEKLMLDPRLDCFQQNMLSPKMIIGDPSVDLNVRESSRILRKQMGGCPNLRSVGKVSMKNKPLSIKDKISEWEGKKEIPAQVSLWKEEDQSVKEEPSPVLGVMEKMARHSVAMRDVDNKRLPSCKGPSKEDDRHVGTFKNAGQHVVRKADVKSREEECHFNVGKCTELKDSKKEVQKENISVLSQVKKLEQALKCGSAEAQPQLPGTYYSPHCLQEKTGEDQEPPEGQESTNRIEVSKWLLGLDTEAREPIFGTLEEVKASCVKCRSRNEENVYTEPGLPEKKPFINPLPKPRRTFKHEGEEGWIPPARNKRLLPPLPSIPPPPLPSSPPPSAISRRLRNGKLKANADHRKSYEFEDLLQSSSENGRVDWYAQSKMALTRTLSEENVYEDILVMILALATDAQQLVLKINAIYEARRGKKRVKRLSQSTESSSGRVTDENSESDSDTEEKQRAHSQRLVHVKSRLKQAPKYHTLERDLIEYQERQLFEYFVVVSLHKKQACAPYVPEVTQQFPLKLERSFKFMREAEDQLKAIPQFCFPDAKDWTPVSQFSRKKFYFVLYLLPWFSIPDLPAKENPYEDIETNSHCLGKKCGLNFPASPSSIPGTPTKVPSKPIFFRQNSERRSFKLLDIRKPNRDGTYSPSKISPPSTPSSPDDTFFSLGDSQNDLKQLFEIVQWFGMFQVIQQKPSGKGKRLPEVYCIVSRLGCFNLFSKILDEVEKRRGISPALVQPLMRSVMEAPFPALGRTISIKNFLPGSGTEVMLAGEFWELKSAKGKKLPIXIRTTLQKXMKXLSLQXYCYYKESXLLLSFFSSSTLSKCCHATVALLYPFAWQHTYIPVLPPSMIDIVCSPTPFLIGLLSSSLPRLKELPVEEVLVVDLVNNRFLRQMEDEDSILPRKLQGALEHILEQRNELANEKEEVTLNGKQEASPLNEVVSEAFVRFFVEIVGHYSLFMIPAEKEEKALQREAFRKSVSSKSLRRFLEVFMETQMFGGFIQERELRKQGAKGLFEVRAQEYLETLPSEEQSGVNKFLKGLGSRMKFLHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z850</accession>
    <name>A0A6I9Z850_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rotatin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106557279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931965.1">
        <property type="nucleotide sequence ID" value="XM_014076490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z850"/>
    <dbReference type="GeneID" id="106557279"/>
    <dbReference type="KEGG" id="tsr:106557279"/>
    <dbReference type="OrthoDB" id="9048576at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010457">
        <property type="term" value="P:centriole-centriole cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032053">
        <property type="term" value="P:ciliary basal body organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030791">
        <property type="entry name" value="Rotatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029249">
        <property type="entry name" value="Rotatin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31691">
        <property type="entry name" value="ROTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31691:SF1">
        <property type="entry name" value="ROTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14726">
        <property type="entry name" value="RTTN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rotatin N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931965.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16022" checksum="14E7D5C02361F8E4" modified="2020-10-07" version="1">MKEEVLNLMTNLLKCPSVVEQLVGIGAVEFFTQLRPNVDCSLQAVIDGILDGLFVLPSDVMLDYHTNLSEAGPEANPVILKEPHAGYFHQGKNHSQESEMPPKQPTGRIKDYKISLTAEITPNLLACQVSSSWGFTQEWQRINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z898</accession>
    <name>A0A6I9Z898_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">2-oxoglutarate dehydrogenase complex component E1</fullName>
            <ecNumber evidence="6">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">OGDH</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:12189"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013932010.1">
        <property type="nucleotide sequence ID" value="XM_014076535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z898"/>
    <dbReference type="GeneID" id="106557321"/>
    <dbReference type="KEGG" id="tsr:106557321"/>
    <dbReference type="CTD" id="4967"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000007">
        <property type="entry name" value="2-oxoglutarate dehydrogenase e1 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000001">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF7">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="23">
        <location>
            <begin position="664"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013932010.1"/>
        </source>
    </evidence>
    <sequence length="1034" mass="117335" checksum="A980D94E8191634E" modified="2020-10-07" version="1">MFHLRTCASKLRPLTASQTIKTVSQNKPPPAPRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYYAWLENPKSVHKSWDIFFRNANAGAAPGTAYQSPPLLNTSLSTLAQSLVQAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSVPADIITSADKLDLAAFKERLRVLTVGGFYGLHESDLDKVFHLPTTTFIGGNESALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFSSEEKRTLLARLVRSTRFEEFLHRKWSSEKRFGLEGCEVLIPALKTIIDKSSEKGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDVKYHLGMYHRRINRVTDRTITLSLVANPSHLEAADPVVQGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTYGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNADDPEAVVYVCNVAAEWRSTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAETLISQAVVNQPEYEEEISKYDKICEEAHARSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLTEEDLVHIGKVASSVPVENFTIHGGLSRILKTRGELVANRTVDWALAEYMAFGSLLKEGIHIRLSGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVVWEAQFGDFHNTAQCIIDQFICSGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVFPKLDDFDVRQLYDCNWIVVNCSTPANFFHVMRRQILLPFRKPLIVFTPKSLLRHPEARSSFDEMLAGTRFQRVIPDTSLAAQNPEQVKRVLFCTGKLYYELIRERKTRNMEADVAITRVEQLSPFPFDLLLKEFHKYPNADLAWCQEEHKNQGYYDYVKPRLRTTIDRAKPVWYAGREPAAAPATGNKKNHLTELQRLLDTAFNLDSFKGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X156</accession>
    <name>A0A6I9X156_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">General transcription factor II-I repeat domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106538169</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908076.1">
        <property type="nucleotide sequence ID" value="XM_014052601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X156"/>
    <dbReference type="GeneID" id="106538169"/>
    <dbReference type="KEGG" id="tsr:106538169"/>
    <dbReference type="OrthoDB" id="10061052at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040647">
        <property type="entry name" value="SPIN-DOC_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF11">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18658">
        <property type="entry name" value="zf-C2H2_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="10">
        <location>
            <begin position="629"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="841"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="869"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="897"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="925"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="953"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="592"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="707"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013908076.1"/>
        </source>
    </evidence>
    <sequence length="980" mass="111742" checksum="66D0D825B092E70B" modified="2020-10-07" version="1">MAELRVDIENSCFQKRWEAEYLFVDIEGTAVCLVCRDIMAVFEEYNMRWHYETKHHDRYKNMSVQQKIKTVEELRKNLMSFIKAKTQREAAVKATFIVAEEIAKAAWPFTEGEFLKRCMVKVCEVLCPDQKEAFLNLSLSRNTIAGRIGELATVLQGQLVEKGKDFIAYSLAVDESSDISDVAQLTIFIRGVDPSLCVTEEFLDMKSMHGTTTGKDIFEAVSKCVNDMKLPWDKLTGLTTNGTPSMRGEESGLVGRVREKMKSENCTGELTAYHCIIHQETLCGNILKMEHIMGTVTQTINFIRANGLNHHQFKSFLEEMHSELGDLPYHAAARGISQEKVLSRFFELRLDICQFVESKGKDSTVLRDEKWLCELAFLCDVTKHLAALKLQLQGRDFAITDMYDAVKSFQVKLQLWEAQMQHGNLSDFPCCQTIISQVSTAVFSHAQFADQLSTLHMEFARRFVEFEAQTFDFGLLSNPFSVDVKEAPADIQMELMELQCSDTLKAKYDSVGSAQFARFIPETMPQLRLRAARMLCMFGNTHLCEKVFSVIRANKRAHRSLLTDAHLRSILRVSTAQNLTLNMDELVARKRCQTSRSEKKGQPMKMARVPPDRGEALSDLSATTSQGSVSFEDVVVYFSEEEWALLDSSQRALHREVMLENSRNLAAVGYGQEHKDYQGPSLALFESVEKMFGIQGVLQKRAKSFLKNRRKKSSPSDRNEVVGLPTPQLQQPVGSEKYFGGDKTESAKIVFIQYCTNQTEEEQYERQEYGQNINLSSSLVLHHPSYIQETPYALINCVENFTLNSHLMSDGENHPYECGKGFSQKQLLILPPENPPMEKPYKCPECGKTFPHRCRLTVHEWIHSGEKPYICQVCGKRFRQTGHLSRHRRTHTGEKPYTCLHCGRKFSESSDLTKHVRSHTGERPYKCLECGKSFKCSSHFICHKRSHTGEKPFECSECGKSFSRHNHLSLHKTIHRGEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2G1</accession>
    <name>A0A6I9X2G1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Enkurin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ENKUR</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908516.1">
        <property type="nucleotide sequence ID" value="XM_014053041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2G1"/>
    <dbReference type="GeneID" id="106538532"/>
    <dbReference type="KEGG" id="tsr:106538532"/>
    <dbReference type="CTD" id="219670"/>
    <dbReference type="OrthoDB" id="2123594at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027012">
        <property type="entry name" value="Enkurin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052102">
        <property type="entry name" value="Enkurin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490:SF0">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490">
        <property type="entry name" value="ENKURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13864">
        <property type="entry name" value="Enkurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51665">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enkurin" evidence="7">
        <location>
            <begin position="163"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="47"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908516.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="30130" checksum="30F48DEC65ED0AE1" modified="2020-10-07" version="1">MTAMVMEESIYNLLPQIMEKSQITEKPRFVSAFRPYVKRAIQQSKSPWKTIGPPKVQVPSPKNFLQKHSKEPKLPTKKKDKRSKKAPVLSVPKRTDHPVMGVQCTRNFINTNASNAIVAVPKKPLPVYVDRRQGDRFLLETSGLVPKYLKKKDYGEIPKYVIKRNEDTKKAQEEYDDYVRETLRQKAMKRLTEAERETLLEGLKTNWEEVHQAFQNLSVEIDTLPKKLYKEKLETDMKQLELDIHTIEKHKVIYIANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X489</accession>
    <name>A0A6I9X489_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Suppressor APC domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106538981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909121.1">
        <property type="nucleotide sequence ID" value="XM_014053646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X489"/>
    <dbReference type="GeneID" id="106538981"/>
    <dbReference type="KEGG" id="tsr:106538981"/>
    <dbReference type="OrthoDB" id="10035013at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026828">
        <property type="entry name" value="SAPC2_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14907">
        <property type="entry name" value="FI14130P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14907:SF4">
        <property type="entry name" value="SUPPRESSOR APC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11414">
        <property type="entry name" value="Suppressor_APC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013909121.1"/>
        </source>
    </evidence>
    <sequence length="107" mass="12459" checksum="07E85199E887001F" modified="2020-10-07" version="1">MAVQGGYTILIIPLGTALSNLHALRFFLWMKQMQQLERERDALLQGLEMVNQVRDWLQCHLLEAQKRQKHMGAQAVSQDGRPFCLPRLWWVIRHKRGIASRAPIKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7M3</accession>
    <name>A0A6I9X7M3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Clathrin heavy chain 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106537708</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907378.1">
        <property type="nucleotide sequence ID" value="XM_014051903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7M3"/>
    <dbReference type="GeneID" id="106537708"/>
    <dbReference type="KEGG" id="tsr:106537708"/>
    <dbReference type="OrthoDB" id="2113814at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045334">
        <property type="term" value="C:clathrin-coated endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000005">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000082">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.730:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF7">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="16"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="165"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="312"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="458"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="607"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="753"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="902"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013907378.1"/>
        </source>
    </evidence>
    <sequence length="1154" mass="133406" checksum="AF70F3853AC95083" modified="2020-10-07" version="1">MRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDQELINVCNENSLFKSLSRYLVRRKDPELWASVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARRKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYEEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWGELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNFFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDRLYKDAMQYASESKDTELAEELLQWFLQEGKQECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLSKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9T6</accession>
    <name>A0A6I9X9T6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING finger protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">RNF17</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Seems to be involved in regulation of transcriptional activity of MYC. In vitro, inhibits DNA-binding activity of Mad-MAX heterodimers. Can recruit Mad transcriptional repressors (MXD1, MXD3, MXD4 and MXI1) to the cytoplasm. May be involved in spermiogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with MXD1, MXD3, MXD4, MXI1 and PIWIL1. Self-associates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912369.1">
        <property type="nucleotide sequence ID" value="XM_014056894.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541443"/>
    <dbReference type="KEGG" id="tsr:106541443"/>
    <dbReference type="CTD" id="56163"/>
    <dbReference type="OrthoDB" id="5800423at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20414">
        <property type="entry name" value="Tudor_TDRD4_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20415">
        <property type="entry name" value="Tudor_TDRD4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20418">
        <property type="entry name" value="Tudor_TDRD4_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000114">
        <property type="entry name" value="RING finger protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047845">
        <property type="entry name" value="RNF17-like_TUDOR_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047847">
        <property type="entry name" value="RNF17-like_TUDOR_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047850">
        <property type="entry name" value="RNF17-like_TUDOR_rpt5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16442">
        <property type="entry name" value="RING FINGER PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16442:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Tudor" evidence="17">
        <location>
            <begin position="578"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="17">
        <location>
            <begin position="814"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="17">
        <location>
            <begin position="1070"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="17">
        <location>
            <begin position="1314"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="953"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="14"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="957"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="976"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013912369.1"/>
        </source>
    </evidence>
    <sequence length="1457" mass="165400" checksum="5C40DFBCAC816D8B" modified="2020-10-07" version="1">MELKLNNVFRAKPVENDSKRMQDIEKALNKAKNNLEQLKVLEELLQQLITETKQEESKIADVITAKFTDCMASLNSRKRKIEAELVMNTSYYVTDIHSIQVALIQKRSNLIDAVKKAKELKTTNSLESCHSLNQALCNLNVSVEEEVLKLDNLKKRTLPRFCMDDEIIHVLANIGKFAYGATNVYNFEENSLKMNNELGTSHQVNVNLVKEVNVIPPSEESDKCFGGAHLQAMSPLSIQKHTAALSQATSIPDVIIEEIIEDDQEKVPEYVIVSCVINPCHFYVRKVSQKKTAIYLEKVLKHYCRNNYLSPTDILELGARILIKSKEHGMWCRAEIMELIPLLKANEEKACGLTKHKICDIAIMKVFLIDFGHPEALIISRVPNEVTVNPEHVTLKYMMIEDLCLVVRKMDLSLETRLRGIRNLALQCSLKGIVPKDSEKGWGRTARTEFLRMVNSKAVLMKIFREENGVLIVDLMKPPANKISSDMPVSLRDALVFLDLASFQNEFSDWSKNTVPLEYYPPIMPRDNTEVAAVVSYINSPGDFYIQLIEQGPEFAAFLKKVEEVYENEAGPDLQMLCPTHGQPCVAKFEDDGVWYRARVIGLPGHQEVEVKYVDYGNTAKINIKEMRKIKDEFLVLPEKAIRCKLAYIKPCKGATEWTVQSKDRFEQLIQDKCMLCFVTEKSEDNVLSVELYQSVCVSPKQSASVNNVLVEEGLASYIIGNNKMIVTPYNEIWDPDLEDICKPEKSSLQPEIEDLTEMEDLVLESNKELQVQINHVVSPSKIFVLFMLSEKILKRLQEKMNATYFETENKAIKWEVDMNCAAYVCEQNQWQRGQIIRIVSEKVVEVFLIDLGIVQTMDITCLRELEQNLKTIRPLAVECSLTNIRPSGGTEQWTATACDVLKSYLTGAVVNLIIQDTNLSPLPVKIFCKHERHCTDISEYMIQEGLALRKRAPKNDPNQASSNELQNNVDINKVASEKESPSKLEKPFGPSDTDSKQQELKVSVAKPLVVEAYKPPALPTLDCFSAVVSCVSDNGTIYVIPTSQEQKLKELMCNIQDNVKGLGLLKPYNWKSGEACVVRAADTMWYRGEVKDVGAGIVKVHYVDYGYTEKIPPCHLYPTILYAEIPPFSIPCHLYKTVPVGNIWQHDAVELLKEVLTKRSVKIRIMEQSNPPWGKVSVKLWFSGMSLSYFMAFHKHCITEEDGDSIPTWDSKSECSIETLEENCEISYEGLLKIDLKTSLLPPYTSPSLPILGEHFPVKVTHIVSPNEVYISMVHSNSTSQERDKEDSLDSDTLEKTLAQYNQNIETLPHLTDFQKDMPCLAEYSDGLWYRAKLISFVEFNPISVLVEFVDYGSTKTLPVSRLRQIPRKFMQYPAQAFRVLLAGFKPALHDSTTERIPYCPEWSLDALWAAMNCFESKNLSASSVTHSPEHIVFLYEEGHLFHMKLVEMGFAELTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XA43</accession>
    <name>A0A6I9XA43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein tyrosine phosphatase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PTPDC1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play roles in cilia formation and/or maintenance.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein-tyrosine phosphatase family. Non-receptor class PTPDC1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912469.1">
        <property type="nucleotide sequence ID" value="XM_014056994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XA43"/>
    <dbReference type="GeneID" id="106541517"/>
    <dbReference type="KEGG" id="tsr:106541517"/>
    <dbReference type="CTD" id="138639"/>
    <dbReference type="OrthoDB" id="542013at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14506">
        <property type="entry name" value="PTP_PTPDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000027">
        <property type="entry name" value="Protein tyrosine phosphatase domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049573">
        <property type="entry name" value="PTPDC1_PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="126"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="212"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="375"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912469.1"/>
        </source>
    </evidence>
    <sequence length="760" mass="87185" checksum="B11D34BAF529743B" modified="2020-10-07" version="1">MQGSPRRRLAVNLFSKFFQGRRHSSSDPLFQVLQRRRSSVVEVLSTSTHRVMVAISSVSPEELNAAFHERRRRSRRPTAKYTKVGERLRHVIPGHMQCSMACGGRACKYENPARWSEQEQAVKGLYSSWVTDNILAMSRPSTDLIGRYNIIEQFQRCDIKTVINLQRPGEHASCGNPLEQESGFTYLPEAFMEAGIYFYNFGWKDYGVASLTTILDMVKVMTFALQEGRVAIHCHAGLGRTGVLIACYLVFATRMTADQAILFVRAKRPNSIQTRGQLLCVREFSQFLIPLRNVFATCEPKAHAVTLSQYLIRQRHLLHGYESRHLKYVPKLIHLVCKLLLDLVENRQVIEEELIDMPDLTAEIEETVSQLASTELDKELRRQDSSTSEPFSLSGSNPLHPREHDCDPLCKSRTADHFLSLSRSKRRLSYSETDLRKFAFITEQGETLWSVPVQAPLCNKSNPPSKVNDCQPLHIELNQEVLIRNTFTFSTQGKFNPGKNEDDSSACHRSRHTKRVLRSKTFSSGLSYHQKEKEEDEEEEEEELKALNYNFVRKDIYGRRISSEDSESGTYDIKDLSDAIPHIIVQSELTQEARRILAAKALASLNEFMEAEEVKSKVEMWQKELNSQNGAWDKICAERDPFILSNLMWSWIEQLKEPLLTKNDVDVLAKNNTDFQEAFKLLEKGKYHTILCILNCVVNLQTIPTDVEELLLNRAIKAFTKQMNYDSESGSEVYSILKNVFKQILEHQRQYANEETGKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAG2</accession>
    <name>A0A6I9XAG2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Raftlin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RFTN2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the raftlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908233.1">
        <property type="nucleotide sequence ID" value="XM_014052758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAG2"/>
    <dbReference type="GeneID" id="106538309"/>
    <dbReference type="KEGG" id="tsr:106538309"/>
    <dbReference type="CTD" id="130132"/>
    <dbReference type="OrthoDB" id="9942562at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028169">
        <property type="entry name" value="Raftlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17601:SF1">
        <property type="entry name" value="RAFTLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17601">
        <property type="entry name" value="RAFTLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15250">
        <property type="entry name" value="Raftlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908233.1"/>
        </source>
    </evidence>
    <sequence length="425" mass="48012" checksum="6C8740BD581969F7" modified="2020-10-07" version="1">MGCGLRKLEDSDDSSPGKIFSTLKRPQVETKTDSAYEYVLLDFTLEVISNPEIIKICSILDIAKNVEKYYNKGYVVGAIHPVLLSPGQRRHFPASHIYRVVLVRLKFSHKNPPMREPRQPRLVIEECPLTYEKLTNETVKEVLEKVNDAAKRGMKFVGFITQSCSQSKICNGANLGEDMESDSTLCRRNREQRNHDSEENVRILNEGILSGQSSESGIQEEIHPDVKPVQKTDFQDGREGCPSPNKFKEKEETKLYTVFNVFEDDSISWTYHDGILSLKVSRKGPVISTLDADWLDLTTFYYRQGLSLIDSFVCWEASKADFGPKSLEGLFIYEEEPGFPGSERKGNDAIVVEQWTVIEGSEIKTDYGPLLHTLAEFGWLLTCVLPTPVIRLDSEGNLATKQIVFLQRPAMGNPAVQIPEVRITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAX0</accession>
    <name>A0A6I9XAX0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel subfamily G member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KCNG4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911451.1">
        <property type="nucleotide sequence ID" value="XM_014055976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAX0"/>
    <dbReference type="GeneID" id="106540740"/>
    <dbReference type="KEGG" id="tsr:106540740"/>
    <dbReference type="CTD" id="93107"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000024">
        <property type="entry name" value="Potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000019">
        <property type="entry name" value="Potassium voltage-gated channel, subfamily G, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF167">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY G MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="431"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="58"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013911451.1"/>
        </source>
    </evidence>
    <sequence length="509" mass="57798" checksum="8406573904BB6B82" modified="2020-10-07" version="1">MPIPAGSKEQDYGIASSKSWSHLLPIAEVKAPPAKGIHYQKVRRISCSDQLRDFDGKEEVMINVGGSKYLLPWSTLDEFPSSRLSKLKSCNSYEEITQVCDDYDEDTREFFFDRNPSAFGMIVSFLAAGKLMLLHNTCVLSFREELKYWGIAESRLENCCFQKLLQKVEELSEEHKEDELQRVKLVACAQKEETRYGRFMNNLRDMVENPQSGLPGKIFACLSVLFVATTTISLCVSTMPDLREEEGRGECSQKCYYIFIVETICVAWFSLEFCLRFIQAKSKCQFFKGPLNIIDFLAISPYYASLIMGEDEISEHDHHRPSGSSYLEKVGLVLRVLRALRILYVMRLARHSLGLQTIGLTVQRSAREFGLLMLFLCVALTLFSPLVYLAETESGKVLEFTSIPASYWWAIISMTTVGYGDMVPRSVPGQMVALSSILSGILIMAFPATSIFHTFSHCYAELRKRHKRLHAQLTRIKSANAVSERDTFNESESFASDDSEYPTKYIFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC53</accession>
    <name>A0A6I9XC53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">EF-hand calcium-binding domain-containing protein 4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">CRACR2B</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913163.1">
        <property type="nucleotide sequence ID" value="XM_014057688.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913164.1">
        <property type="nucleotide sequence ID" value="XM_014057689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC53"/>
    <dbReference type="GeneID" id="106542072"/>
    <dbReference type="KEGG" id="tsr:106542072"/>
    <dbReference type="CTD" id="283229"/>
    <dbReference type="OrthoDB" id="9837699at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="82"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="308"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913164.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="49010" checksum="E27BE5E7E6AB4FD4" modified="2020-10-07" version="1">MQRSGKKHSKTLHQEDKEVNGMTEGVSRSLSLPSPLLCLSDEGNTQVEMLEKAQELFRLCDKEEKGFITRFDMQRLQSELPFTTEQLEAVFDSLVQDNNGYLTPVEFSVGLGKFIGNEPHQNSENASHEETFESSWSEELDQMDEGEKLFCSVLEQFEASQIFEDQGEVQELWVRIRKEAPELLARFEEFLFHISSYIKELHHEKESMELVLKRRETDHDREIRCLYEEMEQQIKAERERLVQQESTRLEKNNMFQKELQNKEQEFEKILYRQKKLEHQLHCQHSEQLEMRIRNERLQILSENLLDQLERTKWDLEVAQSRLQELQKEAQAEQEQKNRDIFQASKNMQKEKQSLLRQLELLREMNKNLRDERDSVEAQKMVSLKKNILIPSHHSFACCHCCHHLGTFHLPGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFG0</accession>
    <name>A0A6I9XFG0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heterogeneous nuclear ribonucleoprotein A1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HNRNPA1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912911.1">
        <property type="nucleotide sequence ID" value="XM_014057436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFG0"/>
    <dbReference type="GeneID" id="106541878"/>
    <dbReference type="KEGG" id="tsr:106541878"/>
    <dbReference type="CTD" id="3178"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12761">
        <property type="entry name" value="RRM1_hnRNPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12582">
        <property type="entry name" value="RRM2_hnRNPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000048">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein a1 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000429">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein A1-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034516">
        <property type="entry name" value="hnRNPA1/3_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021662">
        <property type="entry name" value="HnRNPA1/A2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034845">
        <property type="entry name" value="hnRNPA1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026:SF2">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026">
        <property type="entry name" value="HOMOLOGOUS TO DROSOPHILA SQD (SQUID) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11627">
        <property type="entry name" value="HnRNPA1_LC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="118"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="324"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="335"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013912911.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="38145" checksum="463CB6CF20B3E1BB" modified="2020-10-07" version="1">MSKSEGSSDYGCDLSPNASPKEPEQLRKLFIGGLSFETTDESLRIHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYSTVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSSSGSFGSGRGGGFGGGDNFNRGSNFGGRGGFGSRSGGGGGGGGGGGGGYGSSGDGYNGFGNDGYGGSGPGYSGGSRGYGGSGSYDGYNNGGGGFGGGSGGSNFGGGGSYNDFGSYNNQSSNFGPMKGGNFGGRSSGPYGGSGGGYGGSGSSSSYGGGRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHW9</accession>
    <name>A0A6I9XHW9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106543779</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915334.1">
        <property type="nucleotide sequence ID" value="XM_014059859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHW9"/>
    <dbReference type="GeneID" id="106543779"/>
    <dbReference type="KEGG" id="tsr:106543779"/>
    <dbReference type="OrthoDB" id="9889152at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0552">Olfaction</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="39"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013915334.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="35275" checksum="ED5A043A798C9891" modified="2020-10-07" version="1">MGNSSRVTEFILRGLTDDPHLQLILFVTFFILYAITLLGNAGMIALIWISPQLHTPMYFFLTHLSFVDICCSSAITPQFLCHLLKEKKDISFVGCFTQIYFYGALSTAECFLLAIMAYDRYVAICHPLVYLVIMSQLKCVQLVVISYIIGLLHSLVHIIPASRLFFCGSNIIKNFFCEGPALLQLACSDISLNNLLKLVFIGFTLIATILAILTSYIYVLITILKMHSAKARRKAFSTCTSHLMVITIFYGGLAFVYAQPGKSHYTNQIASVLYVIVTPMFNPLIYSLRNQEVKVAFRKILGEKVALLFYTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJE1</accession>
    <name>A0A6I9XJE1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rho GTPase-activating protein 20-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106542764</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914057.1">
        <property type="nucleotide sequence ID" value="XM_014058582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJE1"/>
    <dbReference type="GeneID" id="106542764"/>
    <dbReference type="KEGG" id="tsr:106542764"/>
    <dbReference type="OrthoDB" id="9994905at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13319">
        <property type="entry name" value="PH_RARhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000217">
        <property type="entry name" value="Rho GTPase activating protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047887">
        <property type="entry name" value="ARHGAP20_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179:SF28">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE ACTIVATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22286">
        <property type="entry name" value="RHG20_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="196"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="784"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914057.1"/>
        </source>
    </evidence>
    <sequence length="883" mass="97124" checksum="B1A58FC26EA370E2" modified="2020-10-07" version="1">MEAMSPQQDHVGQGQSSSLTAGNRRSAAPEAKKQKMKSLAQRRQSAPSLVLSKALTKARTSAREGCFSPISPETCPLVQSFLSPARAFLLEGHVQLKTGLQTQERHLFLFTDILVIAKSKSQLHFKLKNQARLSEMWTASCLEEVCESSTNADRSFVLGWPTTNCVASFSSVEQKEKWFTSLQSRIKEEKAKDYPKSIPLKILAKDIGNCVYSKTLTITNLDTANDVILLALQQLGINGSEKDYQLWVNSGKEDAPYPLIGKCTKSVYVSVLVQFLIENCCRIFGEELTSVFADIMPKNDNREDGSDLSSFHLNDSSYDSLENELNDCADPAFDNLFKKRGKENRSRDSVLTLSDCDLDSPEAGDAQIKLSIKSQPVSISVGFHHKSSSREHSENESLCSVTSGYSSTAISDVLKSSRRHRRCSEPTIGLLDSKFTPLGGFHESAVRKASCDAVLSHTNEDYLEQFRSLQVEGQKLINQNLVMGIEVSSKDGPESRISEKKDVSNRLLLPPPLRLNICSRTSCSSLSSPGTSPSGSSMSSLDSAFSQFSDYSVFTPTEASSPLDCASQPQRKFGEASPDFNPFSGGHLGSGVSAFSNQANNNQTSSLIPIKKDNLEWLPHKNPVTLHPSTWLKSGASTMKNWTLRKRERASKQDEKKNTSVKITLESTTFPSESNLAQGQQGTGNVCATDVNSVAGHLGNGSPQDIGQFTSLPFCVMEAQEKRLKALELLAEQSKEEEEEENSTVFLPHKPPPPSLKPQSQNKPKPEADLGADHQGVTERKLNETARFPGVAVQQAADSKTDKSSLSRQPKDADINLENECYASSHPSVKAEDECSFQMAPNHSFSKTPEGTDANKMEWLKKLCSDPKFKDTDQNFFTEESYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK33</accession>
    <name>A0A6I9XK33_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Low density lipoprotein receptor adapter protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106542942</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914287.1">
        <property type="nucleotide sequence ID" value="XM_014058812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK33"/>
    <dbReference type="GeneID" id="106542942"/>
    <dbReference type="KEGG" id="tsr:106542942"/>
    <dbReference type="OrthoDB" id="9999955at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13159">
        <property type="entry name" value="PTB_LDLRAP-mammal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000137">
        <property type="entry name" value="Low density lipoprotein receptor adapter protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF35">
        <property type="entry name" value="LOW DENSITY LIPOPROTEIN RECEPTOR ADAPTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14719">
        <property type="entry name" value="PID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="6" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="PID" evidence="8">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914287.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="28941" checksum="6E9593F7125549C2" modified="2020-10-07" version="1">MLFNLKYLGMTLVEQPKGEELSATAVKRIVATAKASGKKLRKVTVKVSPRGIVLSDSITNEAIENVSIYRISYCTADKVHDKVFAYIAQSQHNENLECHAFLCSKKKMAQAVTLTVAQAFKVAFEFWQASKEEKEKRDKAILEAESASSLHSDTSSPKAPVTVENLLDLENVTKAPLNTNSVHADGRILGSPSSENNNVWAMNDELDEAFSRLAQSRTNPQVLDISLTSQDIQSAQILSPVDWDKVDSTMETEKDDQLFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK49</accession>
    <name>A0A6I9XK49_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Poly [ADP-ribose] polymerase 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PARP12</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914381.1">
        <property type="nucleotide sequence ID" value="XM_014058906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK49"/>
    <dbReference type="GeneID" id="106543018"/>
    <dbReference type="KEGG" id="tsr:106543018"/>
    <dbReference type="CTD" id="64761"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057602">
        <property type="entry name" value="Zfn-CCCH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF6">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23466">
        <property type="entry name" value="WWE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25261">
        <property type="entry name" value="zf-CCCH_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="14">
        <location>
            <begin position="354"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="15">
        <location>
            <begin position="469"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="446"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013914381.1"/>
        </source>
    </evidence>
    <sequence length="664" mass="76188" checksum="4741CA89E7CF5727" modified="2020-10-07" version="1">MDAGFAQEIFRRVCAAGGSLKVSEIKGRLDSGRLDSILQDKTKFTLVTREDPPSAAGSEEHRVVVATTAARLCRDHGKGKCAGDCRQLHLCRYFVYGSCRQQAARKPCKFIHDIKTGHNFSVLKEHGLDVLNSDELCQLLLQNDASLLPEICPYYNKGDGPYGSCTFKKICVKLHICQYYLQGECRFGSNCRHSHDIFNPDCYEKLEKWGMSQALISKIPLFYRNVWDIKNNISSKYRGRLGELEDVRENKVVSTTDIDGLDTICLYHIRKSCSFQDKCTRVHYDLPYRWQVLNGTLWKDMDNMKKIEQAFCDPGNESILQLGGPEQTSFIQYINFSSMTWGSAKIRRLSTPSSVTKPPHYILTTDWIWYWKDEHNLWKEYGKQDGDHFAATVTTFDLEKAYQSEAFTTLKFSAGTQNYEIDFKAMKQKNLKYHTERSVCRRPRFVSKEDVEKRKSSKTEQSKGSATNIPPHWDKTALPELGYKLISLLPSSSEYVKVQTNFQRTLPRVTISAIKRIQNTSLWEVYQWQKEQMKKANGGKDVDERHLFHGTSKKHVDAICQQNFDWRICGVHGTAYGKGSYFARDAAYSDNYCGVNSSSKTMFLAKVLVGDFTMGNSSYLRPPAKNNDSSLFYNSCVNTLLNPSIFVIFEKHQIYPEYLIEYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKU4</accession>
    <name>A0A6I9XKU4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SLIT-ROBO Rho GTPase-activating protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106543209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914621.1">
        <property type="nucleotide sequence ID" value="XM_014059146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKU4"/>
    <dbReference type="GeneID" id="106543209"/>
    <dbReference type="KEGG" id="tsr:106543209"/>
    <dbReference type="OrthoDB" id="5981864at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000020">
        <property type="entry name" value="SLIT-ROBO Rho GTPase activating protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051627">
        <property type="entry name" value="SLIT-ROBO_RhoGAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14166">
        <property type="entry name" value="SLIT-ROBO RHO GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="3">
        <location>
            <begin position="19"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914621.1"/>
        </source>
    </evidence>
    <sequence length="480" mass="56293" checksum="5CE6E43957511F7A" modified="2020-10-07" version="1">MSSQAKFKKDKEIIAEYETQVKEIRIQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRNLEKLAERFSSKIRSSREHHQFKKDQHLLSPVNCWYLVLTQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKAGDISVNLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAVSKYYIHDVSDLIDCCDLGFHASLARTFRTYLSAEYNLETSRHEGLDIIENAVDNLDARSDKHTVMDMCNQVFCPPLKFDYQPHMGDEVCQVSAQQPVQTELLMRYHQLQSRLTTLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESIKSAASETYMSKINIAKRRANQQETEMFYFTKFKEYLNGSNLITKLQAKHDLLKQTLGEGERAECGTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XL43</accession>
    <name>A0A6I9XL43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calglandulin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Troponin C, slow skeletal and cardiac muscles</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TNNC1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the cellular control mechanism of the secretion of toxins from the gland into the venom.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914667.1">
        <property type="nucleotide sequence ID" value="XM_014059192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XL43"/>
    <dbReference type="GeneID" id="106543233"/>
    <dbReference type="KEGG" id="tsr:106543233"/>
    <dbReference type="CTD" id="7134"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990584">
        <property type="term" value="C:cardiac Troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000033">
        <property type="entry name" value="Troponin C, slow skeletal and cardiac muscles"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF47">
        <property type="entry name" value="TROPONIN C1, SLOW SKELETAL AND CARDIAC TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914667.1"/>
        </source>
    </evidence>
    <sequence length="161" mass="18388" checksum="385BD4A77231453D" modified="2020-10-07" version="1">MFQNIGSQVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEDELSDLFRMFDKNSDGYIDLDELKLMLQGTGETITEDDIEELMRDGDKNNDGKIDYDEFLEFMKGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XL89</accession>
    <name>A0A6I9XL89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DnaJ homolog subfamily C member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">DnaJ protein homolog MTJ1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">DNAJC1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">May modulate protein synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via J domain) with HSPA5. Interacts (via cytosolic domain) with ribosomes. Interacts (via SANT 2 domain) with SERPINA3; the interaction delays the formation of the covalent inhibitory complex SERPINA3-chymotrypsin, but does not alter the catalytic activity of SERPINA3. Interacts (via SANT 2 domain) with ITIH4 (via C-terminus); the interaction protects ITIH4 against in vitro cleavage by kallikrein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Microsome membrane</location>
            <topology evidence="15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911598.1">
        <property type="nucleotide sequence ID" value="XM_014056123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XL89"/>
    <dbReference type="GeneID" id="106540853"/>
    <dbReference type="KEGG" id="tsr:106540853"/>
    <dbReference type="CTD" id="64215"/>
    <dbReference type="OrthoDB" id="1420887at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000044">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000180">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 22" id="KW-0732">Signal</keyword>
    <keyword evidence="3 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026960807" description="DnaJ homolog subfamily C member 1" evidence="22">
        <location>
            <begin position="37"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="23">
        <location>
            <begin position="54"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="25">
        <location>
            <begin position="496"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="24">
        <location>
            <begin position="501"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="402"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="461"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013911598.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="64952" checksum="9EA40136C9E4ED1E" modified="2020-10-07" version="1">MRENSQGALGCRAPPWTGLRWPQLLALVLLAPLAFAWESGDLELFDLVEEVPQNFYAFLGVEQDAPSADIRKAYRKLSLILHPDKNKDENAETQFRQLVAIYEVLKDEERRHRYDDILINGLPDWRQPVFYYRRVRKMSNEELALLLFIILTVGHYAVVWSIYLEKQLDELLSRKKKEKKKRSGGKNSDEVKLGVEKSERLVEKPQWRDLLPCKVGIWFYTTVKALPQLFQEARQFYIEYKETKVKEKEEALARAELETLQKEKKPKVKKPKSEFPVYTVAETNAYIPSYDHGTSIEDIEDQMDDWLEDRNKSLKKKAPEWMEEDLSQLTRSMVKFPGGTPGRWEKIAHELGRSVADVTMKAKQIKDSVSSTPGTIKFSELKTLGQSSRSSKANINLPDLIITQRDKEDDDQEEKTKYNYDPETINKTTTDEQETGLNEARHRKRKTIPKAAETILPGTKETTEDKCRGKRQKDFDTGEQEEYSDEEARKKEKCRSSDDLWTQNQQKLLELALQQYPKGTLERWDKIAKCVPGKSKEECMTRYKLLVELIQKKKMAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLR0</accession>
    <name>A0A6I9XLR0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Hexosyltransferase</fullName>
            <ecNumber evidence="16">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">B3GNT2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14389"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:134090"/>
            <dbReference type="EC" id="2.4.1.149"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with B3GNT8; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Golgi apparatus membrane</location>
            <topology evidence="2 16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="16"/>
    <dbReference type="RefSeq" id="XP_013911768.1">
        <property type="nucleotide sequence ID" value="XM_014056293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLR0"/>
    <dbReference type="GeneID" id="106540975"/>
    <dbReference type="KEGG" id="tsr:106540975"/>
    <dbReference type="CTD" id="10678"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008532">
        <property type="term" value="F:N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030311">
        <property type="term" value="P:poly-N-acetyllactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000010">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF25">
        <property type="entry name" value="N-ACETYLLACTOSAMINIDE BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013911768.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="46616" checksum="558F74C94FA6A8C0" modified="2020-10-07" version="1">MIVGRRRLKLLGILMMVNFFIYVIVEVSKNSGQEKNSKQQVILPRSRFWRKYIPHKAYWNKQQEKLEHLYNPIFALLFNMTVEERLSSNNSFLNSCDPDPSLSSDLKDFEDLPDRFKDFFHYLRCRNYSLLVDQPHKCKHKPFLLLAIKSLIPHFDRRQAIRESWGREINLGDITIVRVFLLGQIPPEDNYPNLSDMLKFESETHQDILLWNYRDTFFNLTLKEVLFLKWVSTTCPDAQFVFKGDDDVFVNTHQILDYLKSLTKEKAKDLFIGDVIRDAGPHRDTNVKYYIPHSIYEGSYPPYAGGGGFLYSGDLALRLANISDQVLLYPIDDVYIGMCLQRLGLSPEKHKGFKTFDIEEKQRDNICSYTNLMLVHNRNPQEMIKIWTNLQDPHLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLY1</accession>
    <name>A0A6I9XLY1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">StAR-related lipid transfer protein 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106543439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914932.1">
        <property type="nucleotide sequence ID" value="XM_014059457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLY1"/>
    <dbReference type="GeneID" id="106543439"/>
    <dbReference type="KEGG" id="tsr:106543439"/>
    <dbReference type="OrthoDB" id="10003330at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04375">
        <property type="entry name" value="RhoGAP_DLC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000007">
        <property type="entry name" value="rho GTPase-activating protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000009">
        <property type="entry name" value="StAR related lipid transfer domain containing 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12659">
        <property type="entry name" value="RHO-TYPE GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12659:SF3">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="17"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="4">
        <location>
            <begin position="268"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013914932.1"/>
        </source>
    </evidence>
    <sequence length="468" mass="53254" checksum="E4F1617EFB06E065" modified="2020-10-07" version="1">MKRNKLPNYRGKMVFGVPLIVSVQRTGQPLPQSIQQAMRYIRSRCLDQVGIFRKSGVKSRIQNLRQMSEASPDYVNYEGQSAYDVADLLKQYFRDLPEPVFTAKLTETFLQIYQFVAKEQRLQAVQAAIMLMPDENREVLQTLLYFLSDVASSEENQMTVGNLAVCLAPSIFHLNVSKKESTSPRSVHRRSTVGKPEQKELNENMAATQGLSHMVANCKKLFQIPQEMILQLSSSYLLADAQPLSLPELMEENLQGEIQDSHPVLEASIRGLLKESSERFKGWIGMLGPQNTELSCKKVEDGHPLRLWKVSTEVAAPPHVVLKRILRERHFWDEDLLQGHVVEALDKNTEVYHYVTDSMAPHPRRDFVVLRSWRTDLPGGRCLLVSFSLEHQKLPLEVGVRATVLTSQYLMEPCGSGHSRVIHICQTDLRGRSPDWYNKVFGHLCAMELARIRDSFPVFNPCGPETKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMD5</accession>
    <name>A0A6I9XMD5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitotic interactor and substrate of PLK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MISP</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917064.1">
        <property type="nucleotide sequence ID" value="XM_014061589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMD5"/>
    <dbReference type="GeneID" id="106545128"/>
    <dbReference type="KEGG" id="tsr:106545128"/>
    <dbReference type="CTD" id="126353"/>
    <dbReference type="OrthoDB" id="9449914at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029304">
        <property type="entry name" value="AKAP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042779">
        <property type="entry name" value="MISP/MISP3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18839:SF7">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 2 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18839">
        <property type="entry name" value="MITOTIC INTERACTOR AND SUBSTRATE OF PLK1 MISP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15304">
        <property type="entry name" value="AKAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A-kinase anchor protein 2 C-terminal" evidence="3">
        <location>
            <begin position="593"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="262"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="546"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="613"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="678"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="683"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013917064.1"/>
        </source>
    </evidence>
    <sequence length="799" mass="91399" checksum="5FFFB25321040E29" modified="2020-10-07" version="1">MTQEIPITEEHLPQTEDSICEISLPKMKSSTRDIPFNSPQNTELNYEDQHEQSGQTVVLQQEMRPEGKESEIFDLGQPELKVENGWKMLDPTEDRSWRVMTKSINGRTDHWTPSSDRESKLEVLRSGSHYAIRAYKGEKKPSRLYEEDEEELRYQVPSQDVSPEITKELEDERQEIIRRQVVRKSNTMAEKWSSTNELEAIDTSSLGKVAVEPKKDNLTSFALCFDNPSPDWAIIPIDPENIDTEQINFAAARQQFLKLEKTNPKMLLEPKRHVPSPRELKATSHTYGRKEPQNPEMPRNDKELGESSQRQLRENSIGGDLLILPQREARSYFETNFVSAEKMPSGLEVDMSFQGNVNERTFNKESGNPLARGQDPAKEPEVTNETPIEREIRLTMEREENLWKERGIQRISTRDDLVEIRSKLLLSSPVSSSASSRKGKDKPHTSFYVLREIEQEAKREEDLQKEGRLLGMYDRGLRQELAERRKVFEREEEEVPLPPLGKQKKQGELLETLHGNSATLCRNVVPPGEDRERTASQNVNYSSEIHRPSSVPAFGKKTPGNEPSLKTYPTDSCAKMDLPSRGPPCNRDLKAPRDKFVLKKQHFAVPVRKLQFSFPEDQGPRSLQRKERHPQKPAPREELYTLKTWRPRTSILIDQEIQDALQRELELQEQRRKISLTAQELPSSQVSSQSSAASGDDGRYSVSSSPVFVPNSPVRLPTSPILYRPGLTTNSPESSFGKCQSPIQCRIRQKEDGKVMSGKRFEFMEGGLRQEEEFLAVLIQASQDHEADSSSPKNLFYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP11</accession>
    <name>A0A6I9XP11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphotransferase</fullName>
            <ecNumber evidence="2">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106544085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013915712.1">
        <property type="nucleotide sequence ID" value="XM_014060237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP11"/>
    <dbReference type="GeneID" id="106544085"/>
    <dbReference type="KEGG" id="tsr:106544085"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050796">
        <property type="term" value="P:regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000020">
        <property type="entry name" value="Hexokinase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF3">
        <property type="entry name" value="HEXOKINASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase C-terminal" evidence="3">
        <location>
            <begin position="33"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013915712.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="17165" checksum="BF7C0913ABE949FA" modified="2020-10-07" version="1">MNIKDFEMDVVAMVNDTVATMISCYYEDHRCEVGMIVGTGCNACYMEEMENVELVEGNEGRMCVNTEWGAFGESGELDEFLLEYDRVVDETSFNPGQQLFEKIIGGKYIGEIVRLVLLKLVNENLLFNGEASEKLKTRGSFESRFISQIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPV3</accession>
    <name>A0A6I9XPV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Runt-related transcription factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Acute myeloid leukemia 3 protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Core-binding factor subunit alpha-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Oncogene AML-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Osteoblast-specific transcription factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Polyomavirus enhancer-binding protein 2 alpha A subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">SL3-3 enhancer factor 1 alpha A subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">SL3/AKV core-binding factor alpha A subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">RUNX2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with DDX5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918059.1">
        <property type="nucleotide sequence ID" value="XM_014062584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPV3"/>
    <dbReference type="GeneID" id="106545883"/>
    <dbReference type="KEGG" id="tsr:106545883"/>
    <dbReference type="CTD" id="860"/>
    <dbReference type="OrthoDB" id="10029800at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007178">
        <property type="term" value="P:cell surface receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002062">
        <property type="term" value="P:chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.720:FF:000001">
        <property type="entry name" value="Runt-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.770.10:FF:000001">
        <property type="entry name" value="Runt-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.770.10">
        <property type="entry name" value="Signal Protein Aml-1b, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000040">
        <property type="entry name" value="AML1_Runt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013524">
        <property type="entry name" value="Runt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027384">
        <property type="entry name" value="Runx_central_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013711">
        <property type="entry name" value="RunxI_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950">
        <property type="entry name" value="RUNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950:SF7">
        <property type="entry name" value="RUNT-RELATED TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00853">
        <property type="entry name" value="Runt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08504">
        <property type="entry name" value="RunxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00967">
        <property type="entry name" value="ONCOGENEAML1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51062">
        <property type="entry name" value="RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Runt" evidence="23">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="121"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="283"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="322"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013918059.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="37508" checksum="B4DCDE3C61D48662" modified="2020-10-07" version="1">MVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVLTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSERLSDLGRIPHSSMRVGVPTQSPRPPLNSAPSPFNPQGQSQITGASELSPFSDPRQFTSISTLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYPGSSQNQSGPFQTSSTPYLYYGTSSGSYQFPMVPGGDRSPSRMLPPCTTTSNGSTLLNPNLPTQNDGVEADGSHSSSPTVLNSSGRMDESVWRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ02</accession>
    <name>A0A6I9XQ02_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein C11orf65 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">LOC106544202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2 3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915881.1">
        <property type="nucleotide sequence ID" value="XM_014060406.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915882.1">
        <property type="nucleotide sequence ID" value="XM_014060407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ02"/>
    <dbReference type="GeneID" id="106544202"/>
    <dbReference type="KEGG" id="tsr:106544202"/>
    <dbReference type="CTD" id="103186530"/>
    <dbReference type="OrthoDB" id="10253073at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33504">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE) 1 BETA SUBCOMPLEX, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33504:SF2">
        <property type="entry name" value="PROTEIN MFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013915881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915882.1"/>
        </source>
    </evidence>
    <sequence length="309" mass="36394" checksum="2A04FC8C72D21CE0" modified="2020-10-07" version="1">MQDDSKKKYGTPGKRIINPKNLIELLDDKKEDNRNKAAFIIQKTWKKWLDMGVFEYYKELIGFKQYGEPSQLMKYIEPREAEFLDAAAGVHIKFRLGGDKFPPSIYYKIFTHRPIVDLCANSPKDYAKIATKPNLRKLEKGSVNESTSDWYKRIENNGWRLLSIRYWKGVDPLTTKDNTKIQEFHHSPQQRKRNMVKKRKERKIAWLKKMYFGENLQVKTEKPEAIALIQRATEGLIESMEKEGIDQVMEWEVDEMLKWTNALNYDEYVKLWKEVGTSKSSEGFHGKTFAVHSLCNFNLDKVFDRKLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ41</accession>
    <name>A0A6I9XQ41_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein-lysine methyltransferase METTL21D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">VCPKMT</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918154.1">
        <property type="nucleotide sequence ID" value="XM_014062679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ41"/>
    <dbReference type="GeneID" id="106545950"/>
    <dbReference type="KEGG" id="tsr:106545950"/>
    <dbReference type="CTD" id="79609"/>
    <dbReference type="OrthoDB" id="413520at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
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        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF44">
        <property type="entry name" value="PROTEIN N-LYSINE METHYLTRANSFERASE METTL21D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918154.1"/>
        </source>
    </evidence>
    <sequence length="115" mass="13597" checksum="5EC4AE2DB159B7D3" modified="2020-10-07" version="1">MNILCQVLFKPRGEDVTDFLPAPDFILMADCIYYKESLEPLVKTLRDLAGLDTFILCCYEERTVGQNPEVEKRYFELLQRDFELQKVPLGEHDEEYRSEDIHIFIMQKKKMNISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQE2</accession>
    <name>A0A6I9XQE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat flightless-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LRRFIP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LRRFIP family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014062789.1"/>
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    <dbReference type="GeneID" id="106546032"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:Wnt signaling pathway"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="leucine-rich repeat flightless-interacting protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRRFIP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19212">
        <property type="entry name" value="LEUCINE RICH REPEAT IN FLII INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19212:SF6">
        <property type="entry name" value="LEUCINE-RICH REPEAT FLIGHTLESS-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09738">
        <property type="entry name" value="LRRFIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="181"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="382"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="442"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013918264.1"/>
        </source>
    </evidence>
    <sequence length="533" mass="61375" checksum="6FE2781B90BB50B4" modified="2020-10-07" version="1">MGTPGSGRKRTPMKDRFSAEDEALGHIAREAEARLAAKRAARAEARDIRMRELERQQKESSSLYNEPLATTKNYRASLCDDGLYKRYSNHPPSEDSYYSSRASSSRSSPVLEERTDKPYSDLYTRPASRNSATPISGNSSRRGSGDTSSLVDPDTSLSELRDIYDLKDQIQDVEGRYMQGLKELKESLAEVEEKYKKAMVSNAQLDNEKNNLIYQVDTLKDVFEEREEQLAEYYRENEEKTKELERQKHMCSVLQHKVNELKESLCQRDELIEENQRMQQKIESISKEVFDLQETVNWKDKKIGALERQKEYFDCIRIERDELRDELANLKETMKRGEKHGLVIIPNCDINHEPVIGAITVVSQEAAQVLDSAGEGPLDVRLRKLAGEKEELLSQIKKLKLQLEEAQQKSSRNDSTNSDLTGLENGSDLQLIEMQRDANRQISDFKFKLSKAEQDITTLEQNVLRLEGQVVRYKTAAENAEKVEDELKAEKRKLQRELRTALDRIEEMEMTNSHLVKRLEKMKANRTALLSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR11</accession>
    <name>A0A6I9XR11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GBF1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum-Golgi intermediate compartment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014051670.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1"/>
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    </dbReference>
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        <property type="entry name" value="Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="GBF1-like_TPR"/>
    </dbReference>
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        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
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        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23325">
        <property type="entry name" value="TPR_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="11" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="17">
        <location>
            <begin position="705"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1441"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1747"/>
            <end position="1778"/>
        </location>
    </feature>
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        <location>
            <begin position="1442"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1457"/>
            <end position="1480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004399"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013907145.1"/>
        </source>
    </evidence>
    <sequence length="1866" mass="210004" checksum="5D27FB21CAE73DF8" modified="2020-10-07" version="1">MPRRLSTPAKAGPVVNDAGWMEGTTRIVDKNIYTVQGEINTVIGAIKRSARWSTHVRLDEERDPLLHSFSVLKEVLNNVTELSVIEPIVFLRPFLEVIRSEDTTGPITGLALTSVNKFISYGLIDSNHEGTAEGMENLADAVTHARFVGTDPGSDEVVLMKILQVLRTLLLAPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRSSQFKEEPKSYVGTNIRKLKMRAGGMSDSSKWKKQKRSLRNSCHVTKASQGIEQSAANGTGEVNVTDVSSPISSGNSENISSIVSPVTDSGLELSSQICSKDDLTDLQLVKSLGINTTMPLNDFRAREQLELQNECPQREKTPSASVESIPDVLEECTSVTEHSDSVSIHDMDYVNPRGVRFTQCSQKEGTTLVPYGLPCIRELLRFLISLTNPHDRHNSEVMIHMGLQLLTVALELTCVSNCPSLLGLIKEELCRYLFQLLNMERLNLYSASLRVCFLLFEKMREHLKFQLEMYIKKLMEIITMESPKMIYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCANLFEDLSKLLSKNAFPVSGQLYTTHLLSLEALLTVTDQIEARCQAKVLRGIYQQEKEILKPNTEIANIIREAITEELQTKLKSSTAELPETRSSSKDCLVTSHLKQQDCLNSDMENDTEKVIPRKPTRFSCFLPKTQELIMIKNKKKLLISGSEHFNQKPKKGIQLLQEKNLLATPMDNDQVAKWLRENPKLDKKMIGEFVSDRKNVDLLDSFVRTFHFQGLRLDEALRLYLEAFRLPGEAPVIHRLLETFTEYWHKSNGTPFANSDACFALAYAVIMLNTDQHNHNVRKQNVPMTLEEFRKNLKGVNGGKDFDQEMLEDIYYAIKNEEVVMPDEQTGLVKENYVWNVLLHRGATEEGVFLHVPPGSYDHDLFAMTWGPIVAALSYVFDKSLDETVIQKTISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPTVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAELLPKTMVEVEDFVDPNGKISLQREETPSNRGESTVLIFVNWLTLSGTEQSGMRGPSTENQEAKRMALECIKVCDPEKLITESKFLQLESLQELMKALISVSPDEETYDEEDCAFCLEMLLRIVLENRDRVTFVWQAMRDHLYHLCINAMEYCCLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLMMKPSMLNRASHQIAYGLHELLKTNAANIHSGEDWYTLFTLLECIGSGVKPPLALQPTRIENDTGAQSDSEIYHPNETSLDRGYTSDSEVYADHSKQGKLHRSVTDVDVVNSGWLVVGKDDIDNSTMAGLSRLGNSPLVNQYSLTVGMDLGPHNTKSLMKCVESLSFIVRDAAHVTPENFELCVKTIRIFVEASLNGGCKSQEKRGKNHKYDSKSARFKKKAKENSLRHFKTSSQHQHRSTSEDEDEDESVPASYHTVSLQVSQDLLDLMHTLHTRAATIYSSWAEEQQHLETSEKKIEANSRALWINCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQALDALEWESCFNKVLFPLLTKLLENISPADIGGMEETRMRASTLLSKVFLQHLSPLLSLPTFAALWLTILDFMDKYMHAGSSDLLPEAIPESLKNMLLVMDTAGIFHNADSRTGYSDLWEITWERIDCFLPKLRDELFKQTVIQDPVSTLPVEVHLPKASISAQTHSSGEDSRPSTHSASSEKSPELAASDSENACISSHFGSHFASPATPGKMNMATDIPPAVAQQPLILQPLASPLQVGVPRLSLPIILNPALIEATSPVPLLATQQSTDSTISEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS60</accession>
    <name>A0A6I9XS60_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Neurofilament heavy polypeptide-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106544989</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916897.1">
        <property type="nucleotide sequence ID" value="XM_014061422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XS60"/>
    <dbReference type="GeneID" id="106544989"/>
    <dbReference type="KEGG" id="tsr:106544989"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005883">
        <property type="term" value="C:neurofilament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099184">
        <property type="term" value="F:structural constituent of postsynaptic intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045110">
        <property type="term" value="P:intermediate filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23214:SF1">
        <property type="entry name" value="NEUROFILAMENT HEAVY POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23214">
        <property type="entry name" value="NEUROFILAMENT TRIPLET H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="301"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="211"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="312"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="382"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="466"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013916897.1"/>
        </source>
    </evidence>
    <sequence length="694" mass="75158" checksum="8295DAFF0E332E9C" modified="2020-10-07" version="1">TPKEEAKSPDKAKTPVKEKVKSPEKAKTPIKEKPKSPEKDTAPQKGPAKPATKAPEKEVEVPAQPLAEKKASKKEEETAKPETPTKEKTEDQGDKKQSPKAKSPTLDGKKTPAAPKAEDSKGVEEATKPPSKPETGKEVEKGTKDPGPKSPAEEAKAKSPSPTAKKEAEKVKAEEKKEEPKKEKAEKEEEKPKEDAKKTLSPKEPPAGLKDTPKPTKTEKAEKSSGMDPKDSKPAEEKAKKSTVLIPHSLSLFGNRKLLEGEECHFGGGMGFFSFPEIAPKGPSIPVHLKVKSEEKIKVVERSEKETVIVEEVVEKEEEAEEEEEEEEAKEEKEEEEEEEEEEAEEEKEGEEESQEGKEDGEGEEREEAGTGKGSPPTEKTPSSEKEPTLKEEEAKSPEEVKSPAKDEAKSPAKVGSPTKEESQPPAKVVSPTKEFLKIPGVKPVEEATSPIKEESKSPAKPASPAKDESQTPEKLKSPMKEAERPPAVKAPEKVASPTKEDLKSPAKEAEKPPVVKVPEKVASPTKEDLKSPEKPKSPAKEAEKPPAVKVPEKVASPTKEDLKAPEKPKSPAKEAEKPPAVKVPEKVTSPTKEDLKPPAKEAEKPPAVKVPEKVTSPTKEDLKPPAKEAEKPPAVKVPEKVASPTKEDLKPPAKEAEKPPAVKVPEKVASPTKEDLKPPAKEAEKPPAVKVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSN2</accession>
    <name>A0A6I9XSN2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zona pellucida-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106545156</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917092.1">
        <property type="nucleotide sequence ID" value="XM_014061617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSN2"/>
    <dbReference type="GeneID" id="106545156"/>
    <dbReference type="KEGG" id="tsr:106545156"/>
    <dbReference type="OrthoDB" id="9045220at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002199">
        <property type="term" value="C:zona pellucida receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010857">
        <property type="entry name" value="Sp38-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15443">
        <property type="entry name" value="ZONA PELLUCIDA BINDING PROTEIN SP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15443:SF5">
        <property type="entry name" value="ZONA PELLUCIDA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="26"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013917092.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="44165" checksum="A6FDE1736494E2B2" modified="2020-10-07" version="1">MEMDCSHTLFLDLYKSDNETFSGQAPIEEKETELIFVHIGAKEFTVPCKPEKIDVVIGMNPTYIWSLENGENLTLHSGASLILHEFRAEESGRYTCSISFTEEDQLHTMTFSHTVVGYHIRGELEVLLIFQSNSCDKQLTREFVRTLHAQLSQLVFHLRCELLSGSTTCFPTVEKPLDEFNLQVELKVTPFGKDWDKSCYPQIDSMALECYHSAVQKNLQEAKEAMTEFMDKNKNFSLETSNGSDVSFVNTFFNFLEEGKCHEGYGQTQELQSRCPDCCTLCPPGTYSVATTDSCVLCPIGSYSLHYGTIICISCNNNWLTSHPGARTSADCMNMRAVAIAFAVAGRNIVPLLKLMERPKYKKKVLLLLQLMNRPKYKKKLQLLLMTLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSW6</accession>
    <name>A0A6I9XSW6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Alpha/beta hydrolase domain-containing protein 17B</fullName>
            <ecNumber evidence="4">3.1.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 21" type="primary">ABHD17B</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Postsynaptic density membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the AB hydrolase superfamily. ABHD17 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013917176.1">
        <property type="nucleotide sequence ID" value="XM_014061701.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917177.1">
        <property type="nucleotide sequence ID" value="XM_014061702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSW6"/>
    <dbReference type="GeneID" id="106545212"/>
    <dbReference type="KEGG" id="tsr:106545212"/>
    <dbReference type="CTD" id="51104"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099175">
        <property type="term" value="P:regulation of postsynapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000008">
        <property type="entry name" value="Alpha/beta hydrolase domain-containing protein 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF48">
        <property type="entry name" value="ALPHA_BETA HYDROLASE DOMAIN-CONTAINING PROTEIN 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7 20 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="18">
        <location>
            <begin position="90"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013917176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013917177.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="32241" checksum="A90386DF27498B7F" modified="2020-10-07" version="1">MNNLSFSELCCLFCCPPCPGKIASKLAFLPPDPTYTLMCDESGSRWTLHLSDRADWQYSSREKDAIECFMTRTSKGNRIACMFVRCSPNAKYTLLFSHGNAVDLGQMSSFYIGLGSRINCNIFSYDYSGYGASSGKPTEKNLYADIDAAWVALRTRYGIRPENVIIYGQSIGTVPSVDLAARYESAAVILHSPLTSGMRVAFPDIKKTYCFDAFPNIDKISKITSPVLIIHGTEDEVIDFSHGLALFERCQRPVEPLWLEGAGHNDVELYVQYLERLKQFVSQELVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XU45</accession>
    <name>A0A6I9XU45_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 280D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZNF280D</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function as a transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914213.1">
        <property type="nucleotide sequence ID" value="XM_014058738.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542886"/>
    <dbReference type="KEGG" id="tsr:106542886"/>
    <dbReference type="CTD" id="54816"/>
    <dbReference type="OrthoDB" id="10032537at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000298">
        <property type="entry name" value="zinc finger protein 280D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025243">
        <property type="entry name" value="DUF4195"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059074">
        <property type="entry name" value="zf-C2H2_Z280C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057618">
        <property type="entry name" value="Znf_POGZ/Z280C-D-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF34">
        <property type="entry name" value="ZINC FINGER PROTEIN 280D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13836">
        <property type="entry name" value="DUF4195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25414">
        <property type="entry name" value="zf-C2H2_Z280C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25429">
        <property type="entry name" value="zf-POGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="383"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="443"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="79"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="166"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="518"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="801"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="978"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1053"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1111"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1203"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="608"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="807"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="831"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="887"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="926"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1007"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1133"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1175"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1222"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1290"/>
            <end position="1301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013914213.1"/>
        </source>
    </evidence>
    <sequence length="1308" mass="139720" checksum="70AC0F098D78033A" modified="2020-10-07" version="1">MAELFMECEEEELEPWQQKAKPIVVDDDDEDDDEPIFVGEFLSTKPTNTYILNRVNPNSSRIGVQNGAPIRGAVAVSPGFHSSGRPATSTLAVQPQPRVSSISGSSQVLQKPLSGCSSPQEGAKSSAGISQPVNISAPPQSLSRNLHIPASAQSASRPVATVTAQPVALNRVTPDRTSGLTFGVRQNSGGSQYQSGSPMNTAGLSKRPSTSEASSITPKKAKANETGSESESPLLSSVQPPDDLALLEKDVASSNIKNGAPFPQACPKCNIHFNLLEPLKNHIKYCCPDMVHTFPGITKTDCPGLSPKIAEAEKGKLIMLVNDFYYGKHEGNLQQVRQEQKTHTTFKCASCMKVLKSNIRFMNHMKHHLELEKQNSESWESHTTCQHCYRQFPTPFQLQCHIESTHTPHESSTICKICELSFETEQVLLQHMKDNHKPGEMPYVCQVCNYRSSAFSDVEKHFRSIHENTKNLLCPFCLKVIKIGAPYMHHFMRHQKKGIHRCTKCRLQFLTSKEKLDHKSQHHRTFKKPVQLEGLPPGTKVTIRASSGSLQPASVTTSPASTNPSALLLSPGTKAANGKAQNKPAASCKGKAKLKPLSVQKKLLPGTDGSGGSKNHSGGSGSSSGSSGGGSKKANKVANTALNNLRCPSIQKCIECYSDIRTFASHFPSYVRCSLCRYSTSCSKAYVNHMMGFHSTRQTKRFWIYKTHSDELRGTTVVCLNCDFLTDDSGLDKMATHLNESQTHSCQVIVEHVPVGRFTAAAVSGLAKDALLNDEIEEKSEKHLVPTVCEEQQGEACISESPQMKGSVRETHADLPGKENTDTTLAADSEGTERPVPENRFSSEFEACSRSPAIRGALGGLENQEESVPAARSEGKPSLLEEDDPVSGPEPEAACPEESSATSPGKQMEGYLSDVQKEGPVLLSAAATSEPHSCSTDSSIKGSADPENPSLVAAKSKASLPVPEGACASELIGSPSKVGLAEDCCPPSQNDFGPLAAEQDGNTTATESSSGSELPAGSSDSTVPEAARDADATRNNCDVGLLVCKGERIEDAFSSGDSAHSDGSEDLAGEGGEDRIEEGDKAMSVTVQCGGSVSELVGDESSIAELCTFSKNSPTGMVRDPLGNPAADEHEGSPSSSGVKNSTIPELQENVLESSCGKAVFDKQGEAEPTELESNKSSACSEWTGSDVQTKVASLEEAQLADVNMDSTPLVSNEEEVSFEQFLRRRGEPDSAHSDASEQGSIHLEPLTPSEVLEHEATEILQQKGGAPASAKKTGLGSEPADLSSGGGSSPNQEDLTSNQEEANDETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUC1</accession>
    <name>A0A6I9XUC1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tetratricopeptide repeat protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TTC4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TTC4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914278.1">
        <property type="nucleotide sequence ID" value="XM_014058803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUC1"/>
    <dbReference type="GeneID" id="106542932"/>
    <dbReference type="KEGG" id="tsr:106542932"/>
    <dbReference type="CTD" id="7268"/>
    <dbReference type="OrthoDB" id="420195at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21380">
        <property type="entry name" value="CTWD_Cns1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044059">
        <property type="entry name" value="Csn1/TTC4_wheel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18972">
        <property type="entry name" value="Wheel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Cns1/TTC4 wheel" evidence="4">
        <location>
            <begin position="268"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013914278.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="44152" checksum="B22B4BE1E9CFE3EA" modified="2020-10-07" version="1">MAENEEADWDAFLDKFQGPQRYEGAFDPTTWEQEFDQIPIFMKNAPTEINPKENPDLACLQSLIFDGEQSSEEQAMMYKNEGNDYFKEKDYKKAVISYTEGLKKKCSDLELNTVLHTNRAATHFYLGNYRSALNDAVAARKLKPSHLKAIIRGALCHVELKHFSEAMAWCEEGLKLDPQEKKLLEIRTKADRLKRIEERDLRKAKFQEKKEQSQKEALLKAIKDRNIKLSPIPSADEMAISKDLAEVSLDGLSSENGSGLKVYLEDSGSLTWPVLFLYPEHGQTDFISAFHEESRFSDHLVVMFEESPAWDTERKYVPKKLELYFEDEERGDIYQINPETTLLEVLQHKRYFVKAGTPAFLVLVKDAPFSKSYLFGKTVHQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUD1</accession>
    <name>A0A6I9XUD1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">CD226 antigen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CD226</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919714.1">
        <property type="nucleotide sequence ID" value="XM_014064239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUD1"/>
    <dbReference type="GeneID" id="106547171"/>
    <dbReference type="KEGG" id="tsr:106547171"/>
    <dbReference type="CTD" id="10666"/>
    <dbReference type="OrthoDB" id="9937217at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002891">
        <property type="term" value="P:positive regulation of immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002729">
        <property type="term" value="P:positive regulation of natural killer cell cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000304">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042842">
        <property type="entry name" value="CD226"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47011">
        <property type="entry name" value="CD226 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47011:SF1">
        <property type="entry name" value="CD226 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027000889" evidence="15">
        <location>
            <begin position="18"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="17"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013919714.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="38048" checksum="F182CB686A33B6E3" modified="2020-10-07" version="1">MNCLVFLVMAFLQSYQSNVSLMAEGKHVDSIVKLSSTMALKCVCPMNASISQMVWEKEENQTKQLIAAFRLPYDLHIESNYKARVLVTNFTSDNKTLIFSNATEKDVGFYLCSFHTFPHGILEKTIHVVQSGAFEFSNLSIHHLITKPGENVTFKYPFNSDVAVNQMTWERVQPDCIDLIIQCADSEMPIYGSDYQNRVDCISESSFVLLDVTASDYGMYRFSYDKVNGENGTGWIKLSSNNFEPLFTKLHIIFIGSGAFLILIILIILIYRSFCVTKKRKRIKKMRDYKDTCRQSQHQYKNWAGQRSANSSQNPTATEDPIYANWEQVMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUP6</accession>
    <name>A0A6I9XUP6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homeobox protein OTX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">OTX1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919834.1">
        <property type="nucleotide sequence ID" value="XM_014064359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUP6"/>
    <dbReference type="GeneID" id="106547260"/>
    <dbReference type="KEGG" id="tsr:106547260"/>
    <dbReference type="CTD" id="5013"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000142">
        <property type="entry name" value="homeobox protein OTX2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003025">
        <property type="entry name" value="Otx_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013851">
        <property type="entry name" value="Otx_TF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793:SF9">
        <property type="entry name" value="HOMEOBOX PROTEIN OTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03529">
        <property type="entry name" value="TF_Otx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01255">
        <property type="entry name" value="OTXHOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="36"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="38"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="115"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013919834.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="37134" checksum="06EC26C73ABE28D1" modified="2020-10-07" version="1">MMSYLKQPPYGMNGLGLAGPAMDLLHPSVGYPATPRKQRRERTTFTRSQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQSGSGAKSRPAKKKSSPARESSGSESSGQFTPPAVSSSASSSSSSSNSSGPAPGGAALSNSAGAASGSASSAASLSSAPSAVAASSIWSPASISPSAGPEPLASSGASCMQRSVSGASSASYPMSYGQGGGYGQGYPAPPPPPPHSSSYFSGVDCGSYLPMHAPPHAHQLSPIAPSSLAGAHPHTHHPLGQSSNHHHHHHHHHHHQPGYGGPGLAFNSSDCLDYKEAAPTAASSWKLNFNSPDCLDYKDQASWRFQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV49</accession>
    <name>A0A6I9XV49_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">USP53</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919999.1">
        <property type="nucleotide sequence ID" value="XM_014064524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV49"/>
    <dbReference type="GeneID" id="106547382"/>
    <dbReference type="KEGG" id="tsr:106547382"/>
    <dbReference type="CTD" id="54532"/>
    <dbReference type="OrthoDB" id="205782at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010996">
        <property type="term" value="P:response to auditory stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000041">
        <property type="entry name" value="Inactive ubiquitin carboxyl-terminal hydrolase 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052398">
        <property type="entry name" value="Ubiquitin_hydrolase_53/54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975:SF6">
        <property type="entry name" value="INACTIVE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975">
        <property type="entry name" value="UBIQUITIN SPECIFIC PROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="5">
        <location>
            <begin position="30"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="401"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="668"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="803"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="412"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="434"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="674"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1089"/>
            <end position="1098"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013919999.1"/>
        </source>
    </evidence>
    <sequence length="1098" mass="123391" checksum="E67EB56615431B25" modified="2020-10-07" version="1">MAWAKFLRKPGGNLGKVYQPGSVMSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRGLTGHVCQGDACIFCALKTIFSQFQHSREKALPSNNMRHALAESFKDEQRFQLGFMDDAAECFENILERIHYHIVPSNGTDMCTSKSCITHQKFAMTLYEQCVCCTCGASSDPLPFTEFVRYISTTALCNEIDRMVERHERLKPEMFAQLLQAANTTDDFRNCPSNCGQKIKIRRVLMNSPEIVTIGLVWDSEHSDLTEDVIRNLATQLYLPGLFYRVTDEHAKNSELFLVGMICYASRHYCAFAFHTKSCKWVLFDDANVKEVGTKWKDVVSKCLRCHFQPLLLFYANPEGTPVSTEDALQHIIHWSHYKASTGNGEEFGFEKYNFLKSDCVKENGFEETSNQKSKKAQLENTAFTRNYTQPTNVRGSSKLGHNDHREKTKDISRECAQKAAEMKSYSSSLRKNPDRGSRRESGKQKDLSGEIHTNLKAGSPPSGTGFRHHSNERLYGSQGRGSYRHEKVHNQIRPTVQTFSSNKTEDGEKGNRMKTDNTAGYETDSSQDSKDKGSISSSRSRSRGWKPMRETLNVDSIFIDSEKREHSSKSKLNASNKPKHEKEQSSVICSKEIQSQKGLMTIYEDEAKQGGSQNSLDLEKKGNREKITGFVERKVHPDNWQAQRTESGYESSDHISNASANLDSPVTEGNSPVDPNSLKENIFCSDQNLSVRHSGNVLQTFSQLNKNSLEVLKKGEVNMHINYKPSNFSSISHLQIQRTDMPDCNKKSLPTLTVQTALKDHIEKEIKNSSINEQSSLPRSGENVPTETNICSADNSVFSYIGENGIMSERVQNNCESSSTHLQVPCPVSHTKTVNSTPILAFFLQQSKPKPCCTESTPSTGHKTSMYSNKYDDATETVYQNLPPPLPPKKYTLSHLHGIEQESTVELNFTDHPKIEKHVAIATSKSIPEASSFADEERLKAFHCNESSKMSFSPSLLGKDLCTVSNNPLNKRTCFTEPNSSSLDANANDVISLTTYFSVDNCMTDTYRIKYHQRPKLYFADTSGSNKERSLLSSGMQLDTSYHHTSESNHPTAQQKHKSNIGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW08</accession>
    <name>A0A6I9XW08_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">LOW QUALITY PROTEIN: protein TANC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TANC2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Postsynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TANC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917956.1">
        <property type="nucleotide sequence ID" value="XM_014062481.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106545818"/>
    <dbReference type="KEGG" id="tsr:106545818"/>
    <dbReference type="CTD" id="26115"/>
    <dbReference type="OrthoDB" id="5958958at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061001">
        <property type="term" value="P:regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000022">
        <property type="entry name" value="protein TANC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000036">
        <property type="entry name" value="protein TANC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000044">
        <property type="entry name" value="Tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058018">
        <property type="entry name" value="AAA_lid_TANC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058056">
        <property type="entry name" value="WH_TANC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF21">
        <property type="entry name" value="PROTEIN TANC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25520">
        <property type="entry name" value="AAA_lid_TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25521">
        <property type="entry name" value="WHD_TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 9" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="TANC1/2-like AAA+ ATPase lid" evidence="11">
        <location>
            <begin position="673"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="TANC1/2-like winged helix" evidence="12">
        <location>
            <begin position="769"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="992"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1108"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1141"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1174"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1207"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1240"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="1319"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="1366"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="1400"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="10"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="120"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="492"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1495"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1848"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1891"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2036"/>
            <end position="2060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="492"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1536"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1622"/>
            <end position="1641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013917956.1"/>
        </source>
    </evidence>
    <sequence length="2060" mass="227561" checksum="5AE7C00E2F68EE86" modified="2020-10-07" version="1">MFRNSLKMLLTGGKSNRKNRSSDGGSEEPQDRRQASIDYCQSHSGQGGSCAENDCAFEPEYAIPTLTVPEGMQHIRIMEGMSRSLPSSPLLTHQSISVRLQPMKKLTAPLRKAKFVESPRIPESELGSPTLNSTPKLDLDAYCPGETEQELGPPPSVDEAANTLMTRLGFLLGEKVTELQAGNQYNMEGQEDNQTSAVSQXISPCSTLTSSAASPPASSPCFTLPQTSAKDVIAKDCNYGAVTSPTSTLESRDSGIIATLTSYSENVDRAKYGGESSKELGSGGNLKPWQSQKSNIDSCLYRVDENMTASTYSLNKIPDRNLETILSQSVQSIPLYLMPRPNSVAATSSAHLEDLAYLDEQRHAPLRTSLRMPRQSMVGTRTQQDLRVRFAPYRPPDISLKPLLFEVPSITTESVFVGRDWVFQEIDVHLQSTDASVNRGVVIVGNIGFGKTAIISRLVALSCHGTRMRQIASDSPHASPKHVDANRELSLTQQPSAHPSITSGSCPGTPEMRRRQEEAMRRLAAQVVAYHYCQADNAYTCLVPEFVHNVAALLCRSPQFVAYREQLLREPHLQSMLSLRSCVQDPLASFRRGVLEPLESLHKERKIPNEDFIILIDGLNEAEFHKPDYGDTIVSFLSKTIGKFPSWLKLIVTVRTSLQEIIKLLPFHRIFLDRLEENEAIDQDLQAYILHRIQSSSEIQNNISLNGKMDNTTFGKLSSHLKTLSQGSYLYLKLTFDLIEKGYLVLKSSSYKVVPVSLSEVYLLQCNMKFPTQSSFDRVMPLLNVAVASLHPLTDEHIFQAINAGSIEGTLEWEDFQQRMENLSMFLIRRRDMTRMFVHPSFREWLIWRDEGEKIKFLCDPRSGHTLLAFWFSRQEGKLNRQQTIELGHHILKAHIFKGLSKKVGVSSSILQGLWISYSSEGLSMALASLRNLYTPNIKVSRLLILGGANINYRTEVLNNAPILCVQSHLGYSEMVALLLEFGANVDSSSESGLTSLGYAAAAGYLSIVALLCKKRAKVDHLDKNGQCALVHAALRGHLEVVKFLIQCDWTMAGQLQGVFKKSQAIQQALIAAASMGYTEIVSYLLDLPEKDEEEVERAQINSFDSLWGETALTAAAGRGKLDVCRLLLEQGAAVAQPNRRGIVPLFSAVRQGHWQIVDLLLTHGADVNMADKQGRTPLMMAASEGHLGTVEFLLGQGSSISLMDKEGLTALSWACLKGHLSVVRTLVESGAATDHADKNGRTPLDLAAFYGDAEVVQFLVDHGAMIEHVDYSGMRPLDRAVGCRNTSVVVTLLKKGAKIGPATWAMATSKPDIMVILLSKLMEEGDMFYKKGKVKEAAQRYQYALKKFPREGFGEDLKTFRELKVSLLLNLSRCRRKMNDFGMAEEFATKALELKPKSYEAYYARARAKRSSRQFSAALEDLNEAIKLCPNNREIQRLLLRVEEECRQTQVQLQQQPLAASESETQEEELYAVQDILEEEYLEQDVENISVGLQTESRPSPTLPIIQSPPSSPVHRDSAYISNSPLGSHQVFDFRSNNSVGSPTRQGYQPTSPALSPTHQNSQYRPSPPHTSPAHQPSSYRFSPPPIGSQGKDYQSPPPSPLRRGPQYRASPPADSVSVYRAQSSSPVRYQQESNITQLPSRPKSPLSKMTQRSFQMLPLPVAVPQQGLRLQPAKAQIVRSNQSSSAGHCSTVIPTGTYGQVSHSVANKFQSSLAEMAGGQNRLVYQTSIGGIVGDGRPVQHVQASLSAGAICQHGGLTKEDLPQRPSSAYRGGMRYSQTPQIGRSQSASYYPVCHAKLDLERSSLPSSQLGSPDISHLIRRPITVNSNEIKPHPPTPRPLLHSQSVGLRFSPSSSSISSTPNLTPPFRPSASVQQMEIPLKPAYDRSCDELSPVSPTQGGYPSEPARSRTTPFMGIIDKTARTQQYPHLHQQSRTWAVSSVDTVISPTSPGNLPQPETFSPPSSVSSLAFYNKTNNAQNGHLLEDDYYSPHGILANGSRGELLERVSQTSSYPDVKVARTLPVAQAYQDNIYRQLSRDSRQGQTSPIKPKRPFVESNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW18</accession>
    <name>A0A6I9XW18_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neuromedin-K receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">NK-3 receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Neurokinin B receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Tachykinin receptor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TACR3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920379.1">
        <property type="nucleotide sequence ID" value="XM_014064904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW18"/>
    <dbReference type="GeneID" id="106547669"/>
    <dbReference type="KEGG" id="tsr:106547669"/>
    <dbReference type="CTD" id="6870"/>
    <dbReference type="OrthoDB" id="5981855at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004995">
        <property type="term" value="F:tachykinin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902093">
        <property type="term" value="P:positive regulation of flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001681">
        <property type="entry name" value="Neurokn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001013">
        <property type="entry name" value="NK3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR TKR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925:SF1">
        <property type="entry name" value="NEUROMEDIN-K RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01026">
        <property type="entry name" value="NEUROKININ3R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="119"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013920379.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19501" checksum="3848F3FBF56C0CCD" modified="2020-10-07" version="1">VVKMMIVVVLTFAICWLPYHIYFILTGIYENWNRWKYIQQIYLATFWLAMSSTMYNPIIYCCLNKRFRAGFKRAFQWCPFIEVSSYDELELKTTRFHPTRQSSLFTVTRMESSMSVVFESGDGESSKSMRKKGAQEANTNGYSCRSSKASSAVSSYINSPYTSADEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWR8</accession>
    <name>A0A6I9XWR8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">creatine kinase</fullName>
            <ecNumber evidence="2">2.7.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CKB</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>creatine + ATP = N-phosphocreatine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17157"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57947"/>
            <dbReference type="ChEBI" id="CHEBI:58092"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:17158"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013915078.1">
        <property type="nucleotide sequence ID" value="XM_014059603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWR8"/>
    <dbReference type="GeneID" id="106543556"/>
    <dbReference type="KEGG" id="tsr:106543556"/>
    <dbReference type="CTD" id="1152"/>
    <dbReference type="OrthoDB" id="430219at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000026">
        <property type="entry name" value="Creatine kinase B-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000001">
        <property type="entry name" value="Creatine kinase M-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF23">
        <property type="entry name" value="CREATINE KINASE B-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="11">
        <location>
            <begin position="52"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="12">
        <location>
            <begin position="166"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="169"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="333"/>
            <end position="337"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="361"/>
            <end position="366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013915078.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="47588" checksum="953E1F16094D1A3E" modified="2020-10-07" version="1">MGRWRERLHFRQVFRAELARGEERFPFAVPLKVSLLSDRPVMPFSNSHNLSKMNYSAEDEYPDLSLHNNHMASILTLELYTKLRGKQTSSGFTVDDCIQTGVDNPGHPFIKTVGCVAGDEESYDTFCDLFDPVIEDRHGGYKPTDQHKTDLNADNLEGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLSVEALASLEGDFSGKYYALKNMTDAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDGRGIWHNDNKTFLVWINEEDHLRIISMQKGGNMKEVFARFCSGLTQLESLFKSQNYEYMWNSHLGYILTCPSNLGTGLRAGVHVKLPNLSKHEKFGDVLKRLRLQKRGTGGVDTAAVGGVFDISNADRLGFSEVELVQMVVDGVKLLIEMEKRLEKGQCINDLIPAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ54</accession>
    <name>A0A6I9XZ54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">hexokinase</fullName>
            <ecNumber evidence="6">2.7.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106539501</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013909775.1">
        <property type="nucleotide sequence ID" value="XM_014054300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ54"/>
    <dbReference type="GeneID" id="106539501"/>
    <dbReference type="KEGG" id="tsr:106539501"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24126">
        <property type="entry name" value="ASKHA_NBD_HKDC1_meta_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000015">
        <property type="entry name" value="Hexokinase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000001">
        <property type="entry name" value="Hexokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000020">
        <property type="entry name" value="Hexokinase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF28">
        <property type="entry name" value="HEXOKINASE HKDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="18">
        <location>
            <begin position="22"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="19">
        <location>
            <begin position="226"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase N-terminal" evidence="18">
        <location>
            <begin position="470"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="19">
        <location>
            <begin position="673"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013909775.1"/>
        </source>
    </evidence>
    <sequence length="917" mass="102556" checksum="681166D3D5D71ABC" modified="2020-10-07" version="1">MLAVHLVAFYFTKLKEDQIKKVDRYLYQMRLSDDTLHDVMTRFQAEMVKGLGRDTNPTAAVKMLPTFVRSLPDGSEKGEFLALDLGGSKFKVLHVKVAEEGKQHVQMESQFYPTPKEIIQGSGTDLFEYVADCLADFMETKDIKYKKLPLGFTFSFPCRQKKLEEGVLFSWTKHFKVRGVQQTDVVSNLCKALRKHKDIDIEVLALVNDTVGTMMTCGYDDQRCEVGVIIGTGTNACYMEEMSNIDLVEGDEGRMCINTEWGAFGDDASLEDIRTEFDKEIDSGSINPGKQLFEKMISGLYLGELVRLILLKMARKGLLFSGKLSTALCTKGKIETRHVAAMEKNKEGLRNTKEILKELGLDPSEEDCIAVQHVCTIVSFRSANLCATALAAILTRLRENKKLARLRTTVGIDGTLYKIHPQYAKRLHKVVRRLVPNCDVRFLLSESGSGKGAAMVTAVAYRLLSQRKQVNEVLALFKLTRENLIEVRNKMRTELECGLKKETHPAATVKMLPTFVCGTPDGTEKGKYLALDLGGTNFRVLLVKIRSGRNKSVQIYNKIFAIPLEIMQGTGEELFDHIVQCIADFLEYMGIKGARLPLGFTFSFPCRQTSIDKGALVGWTKGFKATNCEGEDVIDLLREAIKRRNEFDLDIVAVVNDTVGTMMTCGYEDPNCEIGLIAGTGSNVCYMEEMKHIEIIEGDEGKMCINTEWGGFGDNGCIDNFRTRYDQEVDSVSLNPGKQKYEKMTSGMYLGEIVRQILIDLTKRGLLFRGQISESLRRRGIFETKYLSQIESDRLALLQVRRILQQLGLDGTCDDSIIVKEVCGCVSKRAAQLCGAGLAAVVEKIRDNRKLEHLKTTIGVDGTLYKLHPHFSRILQETVKELAPQCDVTFMLSEDGSGKGAALITAVAKRMHHIAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0K3</accession>
    <name>A0A6I9Y0K3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Band 4.1-like protein 4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106544653</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916473.1">
        <property type="nucleotide sequence ID" value="XM_014060998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0K3"/>
    <dbReference type="GeneID" id="106544653"/>
    <dbReference type="KEGG" id="tsr:106544653"/>
    <dbReference type="OrthoDB" id="6235974at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM adjacent" evidence="2">
        <location>
            <begin position="14"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916473.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="42775" checksum="6CB8CB5ACF08C988" modified="2020-10-07" version="1">MPENESNSLSRKLGKLGSLGYKHRYSGKTALQMYKDISIQLPRPNQTVERSRSKTYPKRTSKVKQFETHCTSQVITYKESGENEGRAKIIAPSPIKSFKKSRNENSPETQRSKTSAPWEENCPQSGLYNSPSDRTRSPKFPHSRRHPSGGSENEPVQPFRRRSRSRCNTSSGSESENSNREHRKKRHRLRQENDMVDSAPQWEAVLRRQKEKNQTEPTHRRSKHRSRSRSPDVQAKEELWKHIQKDLIDPSGLSEEQLREIPYTKIETQGDPVRIRHSHSPRSYRHYRRSQCSDGERSVLSEVNAKADLVPPLPVTRSSDAHCLTAISQQRRNGSKDSLLEEKIQTSMNNVDGKHSAKTIKIVPASRLKIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0Y6</accession>
    <name>A0A6I9Y0Y6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gem-associated protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GEMIN4</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920021.1">
        <property type="nucleotide sequence ID" value="XM_014064546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0Y6"/>
    <dbReference type="GeneID" id="106547396"/>
    <dbReference type="KEGG" id="tsr:106547396"/>
    <dbReference type="CTD" id="50628"/>
    <dbReference type="OrthoDB" id="9875414at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033265">
        <property type="entry name" value="GEMIN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15571">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15571:SF2">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013920021.1"/>
        </source>
    </evidence>
    <sequence length="1091" mass="123550" checksum="2D7162ACF302D52D" modified="2020-10-07" version="1">MTKSPHPNIFSGNNVEETVVDNIYLPEVSHVVLPHVKLSCRPVTICGEVTILHGGFLLASRLCHPKSLSELAKSDWPVVGKPILEALQEICTSYSCYPPQPNLWKKKAAVVLWSKLILSGVPSDERWKSDAFFSVSNAIPEINHTVLFELFKAINAPRLFTQLLLALPDALCQKELRMFVEYVSKDTSPADAAFFLDVWWEIMKHKRGEEDRLVLLFQNVTHQHVLESEELDQPPKRFKSGPSSVPENSAVHLVLPILTEGLKTIKDNLGSSRMKCYALANLVDVLSALMTTECEAKVLSPQAYLEKISSVVTLWSSDGENRYSKKDLDAKVKEAERSMSLLDMVKLSKQSVFSNLSFLLSLLKEWSSDLLVFLNDPRQVCYESYRLLECLASLQKRSADCGPEDLDQARVMLELSKVIADLLKKVHPETWGKSDAGDLVASVAMVIIDKKMDRHKDVCSVFAKEKSWALSKDWVACLVKNKELFQDPELILKLLETVASLESSKDTSRNQKEQQTKMVMIILECYTELSLPDKNKVIAGVLATWGRQGVSKLMVAFSERFQEELNVAFNQIIKSASGETFKKAILAVSRLVVLNPEGTIKKISNLAIANLGTHQYLAEILCSFPALNFQEPQGQPDVSVHFLLECLKEAVWDRLSFPKEKEQFLEFLVHLMQPSGTSPLLSPPDVIQSLIFPFLKSDSPRIELCLQILDKALKVPSEQSWIHTCHPFPLVFSLCKLLDGFCQYWHQPQDQERCSLETKDLVVTNLAQLCDILLVQKDSLSPQLWTQSVAWLHKKISVLDWTIGLRVKNLFGEHFKNEVPATLFEVCKLPEEEWTSRPLPNYGPGSGLLAWLETCCVSTALREQMLVLLVINVDNPEEVNLFSKGFLVALVQVFPWCSQSEWRRLVHVIKSLLEREILYVPYSLEYVQYLPLLNLRPFAYHLQLSVLLLRAFQFLCGSSGTTWMPTEAWKHVGRLYCLGLSDLLGSVKTIARGQFHSAEEKNVIRELSFVYIQMFCHALHVAAMLPNHGTEEPLLLLSLEILSQYEMLYDLDESLGSALRKANEAHFLESIVENVTNKELRTMLLHKLHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2K1</accession>
    <name>A0A6I9Y2K1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Keratin, type I cytoskeletal 10-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106545311</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917303.1">
        <property type="nucleotide sequence ID" value="XM_014061828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2K1"/>
    <dbReference type="GeneID" id="106545311"/>
    <dbReference type="KEGG" id="tsr:106545311"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF137">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="310"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917303.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="46619" checksum="64B886A8C69CA2D4" modified="2020-10-07" version="1">MQNLNDRLASYLAKVHALEEANGDLEQKIRNWYQKSATHTNEGLYNDSKYNSSIDDLRNKIITASIRNASMILQIDNARLAVDDFRMKYENELFLHQNVQADINGLKKVLDELSMFKGDLEIQIESLIEELAFLKKNHEEEMKNWQGTVAGDINVEINAAPGVDLTAILNKMRTEYEVLAEQNRKDAETWFIEKSKELNMTINVSAKKTTTNKKEIADLRHTLQSLEIDLKTQLSLRQSLESTLAGTEGQYCGQLLQIQGMISNVENQVQQIREDMECQNADYRHLLDIKIRLENEIETYRRLIDGEASGKGMKELSGKKSESVPRSSKDLGSQNLGSEKGSGLRASGSEVRSTKTGTETSTRKTEARNIEFTTKDKEIKKTRVIKTIIEELDEHGRVTSSKIQSVEETQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y396</accession>
    <name>A0A6I9Y396_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PAT complex subunit CCDC47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Coiled-coil domain-containing protein 47</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CCDC47</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Rough endoplasmic reticulum membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCDC47 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014065771.1"/>
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    <dbReference type="GeneID" id="106548407"/>
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    <dbReference type="OrthoDB" id="10039147at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030867">
        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026872744" description="PAT complex subunit CCDC47" evidence="9">
        <location>
            <begin position="21"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="56"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="425"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034746"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00034875"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921246.1"/>
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    </evidence>
    <sequence length="478" mass="55404" checksum="5463C454B3935B51" modified="2020-10-07" version="1">MRSWWFLVTTFFILWGLSLAKFEEFDDDDLVEYDDNDFAEFEDVIEATESPQRIVTAEEEEEEATVELEGQDEGLDFEDADGQESDTESEPYDDEEFEGYEEKPDATLGKNKDPITIVDVPAHLQNSWESYYMEILMVTGLLAYIMNYIIGKNKNNRLAQAWFSSHRELLESNFTLVGDDGTNKEAISTGKLNQENEHIYNLWCSGRVCCEGMLIQLKFLKRQDLLNVLARMMRPACDQVQIKVTMNDDDMDTYVFAVGTRKALVRLQKEMQDLSEFCSDKPKTGAKYGLPEALAILSEMGEVTEGMMDTKMIHFLTHYADKIESIHFSDQFSGPKLMQEEGQPLKLPETKKTLLFTFNVPGSGNTSPKDMEALLPLMNMVIYSIDKAKKFRLNREGKQKADKNRARVEENFLKLTHVQRQEAAQSRREEKKRAEKERIMNEEDPEKQRRLEEAALRREQKKLEKKQMKMKQIKVKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3G0</accession>
    <name>A0A6I9Y3G0_9SAUR</name>
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        <submittedName>
            <fullName evidence="8">Serine/threonine-protein kinase ULK1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106545741</name>
    </gene>
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        <name type="scientific">Thamnophis sirtalis</name>
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            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
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            <taxon>Natricinae</taxon>
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    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013917858.1">
        <property type="nucleotide sequence ID" value="XM_014062383.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="ATG1-like_MIT2"/>
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        <location>
            <begin position="66"/>
            <end position="157"/>
        </location>
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        <location>
            <begin position="170"/>
            <end position="242"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917858.1"/>
        </source>
    </evidence>
    <sequence length="248" mass="28248" checksum="FC133C2ADB7E2C05" modified="2020-10-07" version="1">MGSRWVGHNFATNNDNNFSRRSLLTLCPPLPFQQEHTDILCSLRFTLAFVHYVMELAAIKGSANEMSSSVASEYQLQESVVADQISLLSKEWSYAEQLVLYLKVAELLSAGLQMAIDQIRAGKLCLSSTVKQIVRKLNDLYKSSVTSCHHLNLRLQRWFMDKQKLMDRINSITAEKLIFSHAVQMVQAAALDEMFHHREDCVQRYQKALLLMEGLLNFITEQGDVENINKCKMCIERRLSSLLTGICA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4X8</accession>
    <name>A0A6I9Y4X8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myoneurin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">MYNN</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="myoneurin isoform X1"/>
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        <property type="entry name" value="Myoneurin isoform X1"/>
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    <dbReference type="FunFam" id="3.30.160.60:FF:000816">
        <property type="entry name" value="myoneurin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000267">
        <property type="entry name" value="Zinc finger and BTB domain-containing 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001450">
        <property type="entry name" value="zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002586">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="13">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="361"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="502"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013919328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013919329.1"/>
        </source>
    </evidence>
    <sequence length="613" mass="68738" checksum="6F13553564D75A20" modified="2020-10-07" version="1">MQCSHHCEHLLESLNRQREVGFLCDCTIIIGEFQFKAHRNVLASFSEYFWAIYRDTSESNIFLDQNQVKADGFQKLLEFIYTGDLNLDSWNVKEIHQAADYLKVEEVVTKCKIKMEDFAFIANPSSTEISSITGNIALNQQTCLLTLRDYNNQEKSDAPSVDIAPSPVLTAALEKKSVQPKKRKKAFSSPKHPPSKPVQYTGDVAENSAMEMFLDANKLATEITEQAAQGGDNSELQLTPVVESETFAAQEILVQTMAVKPKQGKPHQNCALKEHCLSNITSDKNSYQLESSGEDLDQKYPKAKPVCNTCGKVFSESSSLRRHMRIHKGVKPYVCQLCGKAFTQCNQLKTHVRTHTGEKPYKCDLCDKGFVQKCQLVFHSRMHHGEEKPYKCDACNLQFATSSNLKIHARKHSGEKPYVCDRCGQRFAQASTLTYHVRRHTGEKPYVCDTCGKTFAVSSSLITHSRKHTGEKPYICGICGKSFISSGELNKHFRSHTGERPFICELCGNSYTDIKNLKKHKLKVHSDSENPLDSIALDPSSFSEQDSIPSEKSLLLESADVKPSDILPLPIGTEDHQVLLPVTDGQSSPSDTLLRSSITGYSEPQFIFLQQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4Y4</accession>
    <name>A0A6I9Y4Y4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cerebral cavernous malformations 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CCM2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919343.1">
        <property type="nucleotide sequence ID" value="XM_014063868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4Y4"/>
    <dbReference type="GeneID" id="106546878"/>
    <dbReference type="KEGG" id="tsr:106546878"/>
    <dbReference type="CTD" id="83605"/>
    <dbReference type="OrthoDB" id="5828470at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13516">
        <property type="entry name" value="HHD_CCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13166">
        <property type="entry name" value="PTB_CCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000004">
        <property type="entry name" value="Cerebral cavernous malformation 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032375">
        <property type="entry name" value="CCM2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026159">
        <property type="entry name" value="Malcavernin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21642:SF4">
        <property type="entry name" value="CEREBRAL CAVERNOUS MALFORMATIONS 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21642">
        <property type="entry name" value="CEREBRAL CAVERNOUS MALFORMATIONS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16545">
        <property type="entry name" value="CCM2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="3">
        <location>
            <begin position="67"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013919343.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="49992" checksum="769AA78E37EEF60E" modified="2020-10-07" version="1">MEEEGKRGKKPGIVSPFKRVFLKGEKGRDKKAQEKVTERRALHTVPLALPDRFEPDVLLNDYIEKEVKYLGQITSVPGYLNPSSRTEILHLIDTAKRAHQLPGQLTHEHDAVVSVSAYNVKLLWRDGEDLILRVPIHDIASVSYIRDDSSHLVVLKTAQDPGISPSQSLCAESTKALASGSLSESGVVPVEACCLVILATENKVTGEELCSLLSQVFHIVYTESTIDFLDRAIFDGATTPTRHLSIHSDDSSTKVDIKDPYETEASTFSFPESLDTAGNSPTSFSAQPSPPAKTASDSELSTTATELLQDYMMTLRTKLSSHEIQQFAMLLHEYRNGASVHEFCVNLRHLYGDSRKFLLLGLRPFIPEKDSQYFENFLETIGVKDGRGIITDSFGRYRWTVRSVSNSTTNGNGATGSSDDQSAPSEGDEWDRMISDISNDIEALGCSLDQDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y829</accession>
    <name>A0A6I9Y829_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Leukocyte receptor cluster member 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Lysophospholipid acyltransferase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Membrane-bound O-acyltransferase domain-containing protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20 21 22 23" type="primary">MBOAT7</name>
    </gene>
    <organism evidence="19 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:35999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73003"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36000"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-octadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36835"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:74243"/>
            <dbReference type="ChEBI" id="CHEBI:133606"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:36836"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = a 1-acyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + CoA</text>
            <dbReference type="Rhea" id="RHEA:37015"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:64771"/>
            <dbReference type="ChEBI" id="CHEBI:75243"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:37016"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CoA</text>
            <dbReference type="Rhea" id="RHEA:33195"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64771"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:33196"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916202.1">
        <property type="nucleotide sequence ID" value="XM_014060727.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916203.1">
        <property type="nucleotide sequence ID" value="XM_014060728.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916204.1">
        <property type="nucleotide sequence ID" value="XM_014060729.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916205.1">
        <property type="nucleotide sequence ID" value="XM_014060730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y829"/>
    <dbReference type="GeneID" id="106544455"/>
    <dbReference type="KEGG" id="tsr:106544455"/>
    <dbReference type="CTD" id="79143"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071617">
        <property type="term" value="F:lysophospholipid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF16">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 20 21" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013916202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013916203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013916204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013916205.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="54410" checksum="8C4413956BD72AE1" modified="2020-10-07" version="1">MSPDEIVYLAVLLTSIPVGFLFKQVGPKTKKYGAAVVGIILILVTCHIHILHSLITILGTWLIINISPRACHHLTLGWTFSYLLFFRTVTYFNYPEPTPFTNAVQLLLTLKLLSVANEVQELIQAKKQEMTTFTKSPMIGTVLQKPGLVEILCYSYCYVGLMTGPFYRYRTYHDWLSQIDPSVIPSWRPMLSRSRMIPIFGIFFLVVSHFFPLDYVRSDAFYERELPFRLFYMMPIFFVFRMRFYVAWLCAECACIAAAFGAYPVTAKSRSGGGPTVEYEPLSHRSADGEASSVAYDYETIKNIDPHGTDFCVKVKDGMRYWNMTVQWWLAQYIYKSAPFHSYVMRSAWTMLISAYWHGIHPGYYISFLTIPLCLAAEGAMESGFLKHLSPSGRLWGDWIQWFLKMRAYDYMCMGFVLLTFEDTIRYWSSVYYCIHVAAVFFFLLGTLLGTQRKRGPPKDKQGAPENPKQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8C1</accession>
    <name>A0A6I9Y8C1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dedicator of cytokinesis protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DOCK1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916665.1">
        <property type="nucleotide sequence ID" value="XM_014061190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8C1"/>
    <dbReference type="GeneID" id="106544801"/>
    <dbReference type="KEGG" id="tsr:106544801"/>
    <dbReference type="CTD" id="1793"/>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08694">
        <property type="entry name" value="C2_Dock-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12051">
        <property type="entry name" value="SH3_DOCK1_5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000044">
        <property type="entry name" value="dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.350:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000057">
        <property type="entry name" value="Dedicator of cytokinesis protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.350">
        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047025">
        <property type="entry name" value="DOCK1_5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047026">
        <property type="entry name" value="DOCK1_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042455">
        <property type="entry name" value="DOCK_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF1">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="9"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="8">
        <location>
            <begin position="426"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013916665.1"/>
        </source>
    </evidence>
    <sequence length="715" mass="82094" checksum="91D1CCC594BD5807" modified="2020-10-07" version="1">MTRWIPTKREEKYGVAFYNYDARGPDELSLQIGDTMHILETHEGWYRGYTLRKKSKKGIFPSSYIQLKEAIVEGKGQHETVIPSELPLIQEVTTTLREWSIIWRQLYIQDSREMFHNVRHMIYDLIEWRSQILSGTLPQDELNELKKKITAKTDYGNRILDLDLVVRDEDGNILDPDQTSTISLFRAHETTSKQVEERLLEEKSQKQNLDISREAKFAATPSFALFVNLKNVVCKIGEDAEVLMSLYDPLESKFISENYLVRWSSSGLPKDIDKLHNLRAVFTDLGSSKDRKREKISFVCQIVRVGRMEQRENNTRKLTSGLRRPFGVAVMDITDIINGKVDDEDKQHFIPFQPVAGENDFLQTVINKVIAAKEVNHKGQGLWVTLKLLPGDIHQIRKEFPHLVDRTTAVARKMGFPEIIMPGDVRNDIYVTLVQGDFDKGSKTTAKNVEVTVSVYDEDGKKLENVIFPGAGDEALSEYKSVIYYQVKQPRWFETIKVAIPIEDVNRSHLRFTFRHRSSQDSKDKSEKIFALAFVKLMRYDGTTLRDGEHDLIVYKAEAKKLEDFSTYLSLPSTKLELEEKGHSMAGKGMQNLGSCTISKDSFQISTLVCSTKLTQNVDLLGLLKWRSNTNILQQNLRQLMKVDGGEVVKFLQDTLDALFNIMMENSESETFDTLVFDALVFIIGLIADRKFQHFNPVLETYIKKHFSATLAYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8S8</accession>
    <name>A0A6I9Y8S8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase RNF139</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">RING finger protein 139</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RNF139</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Translocation in renal carcinoma on chromosome 8 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RNF139</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917450.1">
        <property type="nucleotide sequence ID" value="XM_014061975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8S8"/>
    <dbReference type="GeneID" id="106545423"/>
    <dbReference type="KEGG" id="tsr:106545423"/>
    <dbReference type="CTD" id="11236"/>
    <dbReference type="OrthoDB" id="4348522at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036513">
        <property type="term" value="C:Derlin-1 retrotranslocation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000166">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025754">
        <property type="entry name" value="TRC8_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF163">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13705">
        <property type="entry name" value="TRC8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="301"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="364"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="493"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="540"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="571"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013917450.1"/>
        </source>
    </evidence>
    <sequence length="607" mass="68949" checksum="7830C9DA690CC903" modified="2020-10-07" version="1">MNPGVLASSVVLILSQRSLFKFYMISCAFLLAATSVLVNYYAFLHINFHSAYYTTPFGVQLLPHKGPTLWMALSVLQLAFGIGYVTLLNIESIYSQLIVLDLLLPIAGLIFDLPLHFRQILVFASGSILTLHSLVILFTKMKWVYYSTRYVYLLIRHMYRIYGLQLLLEDTWKRIRFPDVLRIFWLSRLAIQAAVLLYVIKAAETDPSMKFYMISSDSFWELLCSLIISGCDSTLTVLGMSAVISSVAHYLGLGILAFIGSTDEDDRRLGFVAPVLFFILALQTGLSGLKPEERLVRLSRNMCLLLTAVLHFIHGMTDPVLMSLSASHVSSLRRHFPVLLVSACLFLLPILLSYILWHHYALNTWLFAVTAFCVELCLKVIVSITVYILFMIDGYYNVLWEKLDDYVYYVRSSGNIIEFIFGVIMFGNGAYTMVFESGSKIRACMMCLHAYFNIYLQAKNGWKTFINRRMAVKKINSLPEIKGERLQEIDDVCAICYHEFTTSARITPCNHYFHALCLRKWLYIQETCPMCHQKVDIDEKENPNLSNNNAFVAPDDNDPDENPAQGGEQEAPAEDELNDESTESEEETESSTQSSAAVPEQPSEHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAE2</accession>
    <name>A0A6I9YAE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106547571</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome c oxidase subunit 6B.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920270.1">
        <property type="nucleotide sequence ID" value="XM_014064795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAE2"/>
    <dbReference type="GeneID" id="106547571"/>
    <dbReference type="KEGG" id="tsr:106547571"/>
    <dbReference type="OrthoDB" id="1107506at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00926">
        <property type="entry name" value="Cyt_c_Oxidase_VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003213">
        <property type="entry name" value="Cyt_c_oxidase_su6B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11387">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000278">
        <property type="entry name" value="Cyt_c_oxidase_6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013920270.1"/>
        </source>
    </evidence>
    <sequence length="86" mass="10228" checksum="71BFE9F73C5D682E" modified="2020-10-07" version="1">MSEAIRRNIENYKTAPFDSRFPNQNQTRGCWQNYLDFHRCEKAMNAKGSDPYVCQWYKKVYSSLCPSFWVDSWDECRENGSFPGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAN7</accession>
    <name>A0A6I9YAN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neuroendocrine protein 7B2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SCG5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 7B2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920745.1">
        <property type="nucleotide sequence ID" value="XM_014065270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAN7"/>
    <dbReference type="GeneID" id="106547665"/>
    <dbReference type="KEGG" id="tsr:106547665"/>
    <dbReference type="CTD" id="6447"/>
    <dbReference type="OrthoDB" id="9922675at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046883">
        <property type="term" value="P:regulation of hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007945">
        <property type="entry name" value="Secretogranin_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12738">
        <property type="entry name" value="NEUROENDOCRINE PROTEIN 7B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12738:SF0">
        <property type="entry name" value="NEUROENDOCRINE PROTEIN 7B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05281">
        <property type="entry name" value="Secretogranin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0765">Sulfation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026712770" description="Neuroendocrine protein 7B2" evidence="11">
        <location>
            <begin position="23"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="174"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013920745.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24005" checksum="EFF749ED0667A51C" modified="2020-10-07" version="1">MGIAMTSTLLFSLSLLLACSMALTFSHSARSPDRVSEADIQRLLHGVMEQLGIARPRVEYPAHQAMNLIGPQNIEGGAHEGLQHLGPYGNIPNIVAELTGDNIPKDFSEDQGYPDPPNPCPIGKTVDDGCLENTPDTAEFSKDYQLHQHLFDPEHDYPSLSKWNKNLLFEKMKGESKRKKRNVNPYLQGQRLDNVVAKKSVPHFSDEEKDRAETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBU7</accession>
    <name>A0A6I9YBU7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Toll/interleukin-1 receptor domain-containing adapter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TIRAP</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921616.1">
        <property type="nucleotide sequence ID" value="XM_014066141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBU7"/>
    <dbReference type="GeneID" id="106548710"/>
    <dbReference type="KEGG" id="tsr:106548710"/>
    <dbReference type="CTD" id="114609"/>
    <dbReference type="OrthoDB" id="9424455at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035663">
        <property type="term" value="F:Toll-like receptor 2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035662">
        <property type="term" value="F:Toll-like receptor 4 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000343">
        <property type="term" value="P:positive regulation of chemokine (C-X-C motif) ligand 2 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032760">
        <property type="term" value="P:positive regulation of tumor necrosis factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017279">
        <property type="entry name" value="Tol-interleuk_rcpt_adapt_Tirap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22662">
        <property type="entry name" value="TIRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22662:SF0">
        <property type="entry name" value="TOLL_INTERLEUKIN-1 RECEPTOR DOMAIN-CONTAINING ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="2">
        <location>
            <begin position="82"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921616.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25633" checksum="FB5ADCF58050423A" modified="2020-10-07" version="1">MEEIEKYQAKMGKNLGWFKTLLQKPRKRPGASNGTPGCPLSSPSPPVQRSPSTLPRSLTGSPKLGCSIARIVSHSDSARWSKLYDVCICHSEGDLEFVEALVAYLESQPEHFRCFLQLRDAVPGGAVVTELCNAVQNSHCWVLLITPNFLKDPWCCYQMHHALTEAPMANGRTIPVLKDIDRTDYPRELRCIYYISVVLQENSFRQIKETILRYLLDFCQNTRSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDB3</accession>
    <name>A0A6I9YDB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Synapse-associated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">BSD domain-containing signal transducer and Akt interactor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SYAP1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a role in adipocyte differentiation by promoting mTORC2-mediated phosphorylation of AKT1 at 'Ser-473' after growth factor stimulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts (via phosphorylated form and BSD domain) with AKT1; this interaction is enhanced in a mTORC2-mediated manner in response to epidermal growth factor (EGF) stimulation and activates AKT1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922161.1">
        <property type="nucleotide sequence ID" value="XM_014066686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDB3"/>
    <dbReference type="GeneID" id="106549121"/>
    <dbReference type="KEGG" id="tsr:106549121"/>
    <dbReference type="CTD" id="94056"/>
    <dbReference type="OrthoDB" id="47923at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071364">
        <property type="term" value="P:cellular response to epidermal growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045600">
        <property type="term" value="P:positive regulation of fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="7">
        <location>
            <begin position="166"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00065448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013922161.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="40893" checksum="1C43A32683108E3A" modified="2020-10-07" version="1">MLRGLSGWLGMNWAEEEGKPSPPKGEAETGGEEVPERDAPPPRYEEEGLKGGPDTDPLFNQAKGLGSYIVSFATTATKKISESVAETAQTIKKSVEEGKIENIIDKTIIGDFQKEQEKFVQQQHTKKSETAMPPWVDSNEEETIQQQILALSADRRNFLRDPPAGVQFNFDFDQMYPVAMVMLQEDELLNRMRFDLVPKHVKEEVFWRNYFYRVSLIKQSAQLTALAAQQAVRKEKNGSEEDRQLTDTVRPKMPPVAIKSQLKHQEDDEEISTSPGVSEFVSDAFDACNLNQEDLRKEIEQLVLDDEKKETTTVDEETADWEKELQQELQEYEVVTESEKQDDNWDKEIEKMLQIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF43</accession>
    <name>A0A6I9YF43_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Proline-rich protein 14 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PRR14</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922792.1">
        <property type="nucleotide sequence ID" value="XM_014067317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF43"/>
    <dbReference type="GeneID" id="106549618"/>
    <dbReference type="KEGG" id="tsr:106549618"/>
    <dbReference type="CTD" id="78994"/>
    <dbReference type="OrthoDB" id="6163216at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026320">
        <property type="entry name" value="PRR14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028149">
        <property type="entry name" value="Tantalus-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14522">
        <property type="entry name" value="EMO2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14522:SF2">
        <property type="entry name" value="PROLINE-RICH PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15386">
        <property type="entry name" value="Tantalus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tantalus-like" evidence="3">
        <location>
            <begin position="590"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="389"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="659"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="661"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013922792.1"/>
        </source>
    </evidence>
    <sequence length="706" mass="79244" checksum="FCA2D0646C942926" modified="2020-10-07" version="1">MECTSNGGCSRLPTSEMTTRRRKIIFRPAAEEEGTPSRLREPLDRGRTSLPLSEDEKWSPKKRQRLQQGSPPKADEGRDPCGGMGETLWDVASPIEKKQRKVLAIDLEDFSTTQVPEVPQRCSLLQRPALLPSKKLLQSSFGNETKVSWSRNRQDSTVCRSLLPKLGSRPCKEPQEQLAPMCQKLGEHRLSPPRCRESERLQAKRQRLQNGVPLINGREGTRATPEEEAHRPIPTLEDCQLTQAQQDAGVGQELLENFPPPCRSDHRELPHLPSSMKTPVSSDLHDQEHHTGPSSETSSSTSVSTLLRPSMVPRFRPWGLAPVFQSMRSKLEAFAEIFFSPSKPSLPPIERSPSPSPCPPANGDEAATTPSATPRPGVKIEVKIAISEPRPRKRSSCHEEEREEADRLVVSGRPPIRQWRLSQGEPAPQPHLGRSYSCPDFPRACSWQSVSSVLSPLLQLRQRRHTVCSLEVSRELDRPTLPCLRKEVYPFSSPPARLLPRPLVHTTYCDPSSYSSLTPSCCHEPDPAHPRKASLSPDGGQKVPAVGLDMVASEPLMLSEDETKGSQENTLEKVSCIRIRKTPARHQANLTPMGLPRPVRLNKKVFSLEEIYTNKNYHTPTEKRSFETIFEVPVERKGTLVFTSQRKLKRAIQFQEVGLPRKQRKPHLRKGLRAKGKPAQSQSAKVEELLQQKLAELHDLSEEEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG96</accession>
    <name>A0A6I9YG96_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Voltage-dependent L-type calcium channel subunit alpha-1C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923221.1">
        <property type="nucleotide sequence ID" value="XM_014067746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG96"/>
    <dbReference type="GeneID" id="106549981"/>
    <dbReference type="KEGG" id="tsr:106549981"/>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005451">
        <property type="entry name" value="VDCC_L_a1csu"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01635">
        <property type="entry name" value="LVDCCALPHA1C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923221.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13327" checksum="43FACA42B9709F7B" modified="2020-10-07" version="1">MEGETKEEKIELKSITADGESPASNKSNTDEYQPNENEEKNPYPTAETPGEEDEEEQPEMPVGPRPRPMSELHLKEKAVPMPEATAFFVFSPSNKYVVSCMNLCHHENSPNNMLLSKCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHN7</accession>
    <name>A0A6I9YHN7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von Willebrand factor A domain-containing protein 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923706.1">
        <property type="nucleotide sequence ID" value="XM_014068231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHN7"/>
    <dbReference type="GeneID" id="106550336"/>
    <dbReference type="KEGG" id="tsr:106550336"/>
    <dbReference type="OrthoDB" id="5186at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039891">
        <property type="entry name" value="VWA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21610">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21610:SF9">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923706.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="29596" checksum="65CF1331A9DB1049" modified="2020-10-07" version="1">MGGNVRLWETSLESLEKSLQEWRTMIGQEDGRPVQITIQRESGLDVTSPKHGKIDPDNAPHVGGNTWAGGTGGRDTAGLGGKGGPYRLDAGHQVYQISQAEKDAVPEEVKRAAREMGEKAFKQRLKEIQMSEYDASTYERFSGAVRRQVQSLRIILDNLQAKGKERQWLRHQAVGELDDAKIIDSLTGEKSIYKRRGELDPQLGSPQQKCKRLSLVVDVSGSMYRFNGVDRRLERSMEAVCMVMEAFENYEDKFKVTPRGKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHU7</accession>
    <name>A0A6I9YHU7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 11 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ALS2CR11</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923757.1">
        <property type="nucleotide sequence ID" value="XM_014068282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHU7"/>
    <dbReference type="GeneID" id="106550381"/>
    <dbReference type="KEGG" id="tsr:106550381"/>
    <dbReference type="CTD" id="151254"/>
    <dbReference type="OrthoDB" id="2144823at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031462">
        <property type="entry name" value="CTSRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048363">
        <property type="entry name" value="CTSRT_C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21665">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED TARGETING SUBUNIT TAU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21665:SF2">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED TARGETING SUBUNIT TAU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15729">
        <property type="entry name" value="CTSRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027024619" evidence="2">
        <location>
            <begin position="19"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Cation channel sperm-associated targeting subunit tau C2" evidence="3">
        <location>
            <begin position="13"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923757.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="37405" checksum="72FC775024BF7157" modified="2020-10-07" version="1">MFSLFFIVNIFATYFVFQVPKQKSDPRNRIVLELVGFEGPKDFPRLFGNVTMHLYEVIQKQSFTETCAMRIRNMIFCTAEVQFMFCYGCLGYGYSHQLKLPGADPAQAVAYSMFLRVPPPEDRKDHSSNVIKPRHMDYPAFLSPDLKVTVGSPELESPKETTEQYKTLQEALKEPARERLEKMKKEYRTLKTWREKAEYLDQLILKRGPRSKPQTKLSRFKEIVGKMHTPIPHGSPVPQESDVYLRSEPEVKEVKQVSDYQKFPDIPPDLSNMHLQITAPVPSRVGSDSEESEKSEICTPVEQIELPALTAVPSRPHSLEFSDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIB2</accession>
    <name>A0A6I9YIB2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106550506</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family. AQP11/AQP12 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923947.1">
        <property type="nucleotide sequence ID" value="XM_014068472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIB2"/>
    <dbReference type="GeneID" id="106550506"/>
    <dbReference type="KEGG" id="tsr:106550506"/>
    <dbReference type="OrthoDB" id="1580043at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000018">
        <property type="entry name" value="Aquaporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051883">
        <property type="entry name" value="AQP11/12_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016697">
        <property type="entry name" value="Aquaporin_11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023265">
        <property type="entry name" value="Aquaporin_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21191">
        <property type="entry name" value="AQUAPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21191:SF8">
        <property type="entry name" value="AQUAPORIN-12A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017529">
        <property type="entry name" value="Aquaporin_11/12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02025">
        <property type="entry name" value="AQUAPORIN12"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013923947.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="33356" checksum="2A3816E52AD6D104" modified="2020-10-07" version="1">MSGLNVSIIFFFSVVTLCEFARRFFKRIFPSKVYFCLAGELANSFQLCACYLELKMLREIGPWGGGFGPDVSLTLLFVIFLAHGASFDGTAANPVASLQEFLALDCSFVGSIVKILAQFLGMEAACAFTKKYWSKEFTHFHMIQNLMAQDCSSSLNSSISQGIFVEAMGSFLLQLIILKLHTCSPMYRVPIVALSITALTYTAAPVTGAFFNPALASAMTFSCSGNSLLEYMQVYWLAPISGMLLALFVYQGNIPRLFQTNLLYSQKKKYRIFKGKVTSNSVAEERQTKTEKKNSRSGLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIR0</accession>
    <name>A0A6I9YIR0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribonucleoside-diphosphate reductase subunit M2 B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RRM2B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924258.1">
        <property type="nucleotide sequence ID" value="XM_014068783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIR0"/>
    <dbReference type="GeneID" id="106550808"/>
    <dbReference type="KEGG" id="tsr:106550808"/>
    <dbReference type="CTD" id="50484"/>
    <dbReference type="OrthoDB" id="10248373at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030475">
        <property type="entry name" value="RNR_small_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF19">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00368">
        <property type="entry name" value="RIBORED_SMALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924258.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="29037" checksum="336CE39731E457A3" modified="2020-10-07" version="1">MSEPRGSCPGSYGTVRPESSEELPRDGLENDLKSNEEPLLRKNPHRFVIFPIQYPDIWKMYKQAQASFWTVEEVDLSKDLTHWNNLKPEEKYFISHILAFFAASDGIVNENLVERFSQEVQIPEARCFYGFQISIENVHSEMYSLLIDTYIKDPEKRLKCGGEFLTEALPVGLIGMNCILMKQYIEFVADRLLLELGFPKVFQAENPFDFMENISLEGKTNFFERRVSEYQHFAVMSQANNKVFTLDADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIS4</accession>
    <name>A0A6I9YIS4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ADP-ribosylation factor-like protein 9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ARL9</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924273.1">
        <property type="nucleotide sequence ID" value="XM_014068798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIS4"/>
    <dbReference type="GeneID" id="106550817"/>
    <dbReference type="KEGG" id="tsr:106550817"/>
    <dbReference type="CTD" id="132946"/>
    <dbReference type="OrthoDB" id="25466at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053254">
        <property type="entry name" value="Arf-like_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46724:SF2">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46724">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="70"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="171"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924273.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="25543" checksum="6FF566F3E677F785" modified="2020-10-07" version="1">MAGSLRQFAFVGTAVAVTGGVAYVLWRNYLSTQEPPGRDDQQSRLRVQEEGTGPPAGQDIPSNKQILVLGLDGAGKTSVLYSLATNHVKRNTVPTKGFNAVCVNTEETKMEFLEIGGSESLREYWKMYMPRVLLLIYVVDSADHERFPVAKTLLHQLVQNNSTLPVMILANKQDLADAYCITDIHDALALSEIGDERKIFLIGTHVAKEGLEISSGLKDTRELITQLVSEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIX2</accession>
    <name>A0A6I9YIX2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-binding cassette sub-family F member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ABCF2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924182.1">
        <property type="nucleotide sequence ID" value="XM_014068707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIX2"/>
    <dbReference type="GeneID" id="106550748"/>
    <dbReference type="KEGG" id="tsr:106550748"/>
    <dbReference type="CTD" id="10061"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000467">
        <property type="entry name" value="ATP-binding cassette sub-family F member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF15">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="82"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="392"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="158"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013924182.1"/>
        </source>
    </evidence>
    <sequence length="620" mass="70518" checksum="4B1CFA27C659A500" modified="2020-10-07" version="1">KKAAKKKEAARRKDAGKARPRPGRGPDEEDEGGGGGGGEANGRPEEPELDEASRALEAFELKKAAARAVTGALASHPNSTDVHVISLSLTFHGQELLSDTRLELNSGRRYGLIGLNGTGKSMLLSAIGKREVPIPEHIDIYHLTREMPPSQKTPLQCVMEVDTERAMLEREAERLAHEDAECEKLMELYERLEELDADKAEARASRILHGLGFTPAMQRKSLKDFSGGWRMRVALARALFIRPFMLLLDEPTNHLDLDACVWLEEELKTFKRILVLISHSQDFLNGVCTNIIHLHNRKLKNYTGNYDQYVKTRVELEENQMKRFHWEQDQIAHMKNYIARFGHGSAKLARQAQSKEKTLQKMMASGLTERVINDKTLSFYFPSCGKIPPPVIMVQNVSFRYSKDGHWIYNNLEFGIDLDTRVALVGPNGAGKSTLLKLLTGELLPTDGMIRKHSHVKIGRYHQHLQEQLDLDLSPLEYMLRCYPEIKEKEEMRKIIGRYGLTGKQQVSPIRNLSDGQKCRVCFAWLAWQNPHMLFLDEPTNHLDIETIDALADAINDFEGGMMLVSHDFRLIQQVAQEIWVCEKKTITKWPGDILSYKEHLKSKMLVDEEPQLTKKMQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIZ6</accession>
    <name>A0A6I9YIZ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SLC4A2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72363"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924186.1">
        <property type="nucleotide sequence ID" value="XM_014068711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIZ6"/>
    <dbReference type="GeneID" id="106550750"/>
    <dbReference type="KEGG" id="tsr:106550750"/>
    <dbReference type="CTD" id="6522"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000004">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001717">
        <property type="entry name" value="Anion_exchange"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018241">
        <property type="entry name" value="Anion_exchange_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF14">
        <property type="entry name" value="ANION EXCHANGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00165">
        <property type="entry name" value="ANIONEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00219">
        <property type="entry name" value="ANION_EXCHANGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00220">
        <property type="entry name" value="ANION_EXCHANGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="767"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="810"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="842"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="900"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="930"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="990"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1030"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1090"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1115"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="15">
        <location>
            <begin position="327"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="14">
        <location>
            <begin position="696"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="14">
        <location>
            <begin position="893"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013924186.1"/>
        </source>
    </evidence>
    <sequence length="1240" mass="137881" checksum="0DB917C83541B9B6" modified="2020-10-07" version="1">MNRGRVSAEHRHVVSGASEGPEPPASPPFAGPQEEAEEEEEKDLSRALRVERFEELLQDRHPRGVDEAGRSYSEEDFEYHRQSSRHIHHPLSAHLPSEGRRKKGSQSKKKKTPRARASVAGETPTIEEEAEGGQEEDGEDDTCDTETEPTPEELLPNSSPEGVQFFLQEDDAERQSPLSGEPACTTPLPSGPRADTGSPQAAGAAKSSSSDPEDSGDAGVAEGGSPPERLLSKSQPGHRSYNLNERRRIGSMTGMEQARYQKVPTDESEALTLVSADLDYMKSHRFEDVPGVRRHLVRKSTKGQVVHLGKDRQEASARLRKHDRKPHEVFVELNELTLDKNQELQWKETARWIKFEEDVEEETDRWGKPHVASLSFRSLLELRRTLSHGAVLLDLDQKTLPGVAHQVVEQMIISDQIRAEDRANVLRALLLKHSHPSDEKEFSFPRNISAGSLGSLLGHHHSANHVGEGPEPTVTDPLIGPVGEHETRVDMEKETLQKEVASPVLSGTITRSKSKHELKLLEKIPDNAEATVVLVGCVQFLDQPTMAFVRLQEAAQLDAVLEVPVPVRFLFVLLGPSSANMDYHEIGRSISTLMSDKQFHEAAYLADDRHDLLQAINEFLDCSVVLPPSEVQGEELLRSVAHFQREMLRRREHQERRLMAGAALEPKSPEEKALLKLKVEEEEEELEADPLQRTGRPFGGLVRDVRRRYPKYLSDFRDALDPQCLAAVIFIYFAALSPAITFGGLLGEKTEGLIGVSELIISTAIQGVLFCLLGAQPLLVIGFSGPLLVFEEAFFTFCTSHGMEYLVGRVWIGFWLVLIVLAIVAFEGSFLVRFVSRFTQEIFAFLISLIFIYETFFKLIKIFQAHPLHGCGNGTSGIPGLENGTISRDASEMTGQPNTALLSLVLMAGTFFIAFFLRKFKNSRFFPGRVRRVIGDFGVPIAILLMVLLDSSIQDTYTQKLSVPSGFSVTSPDKRGWVINPLGSSKVFPVWMMVASVLPAVLVFILIFMETQITTLIISKKERKLQKGSGFHLDLLLIVAMGGFCALFGLPWLAAATVRSVTHANALTVMSKAVAPGDKPKIQEVKEQRVTGLLVAVLVGLSIVIGDLLRRIPLAVLFGIFLYMGVTSLNGIQFYERLHLLLMPPKHHPDVPYVKKVRTMRMHLFTLLQLGCLAVLWAVMSTVASLAFPFILILTVPLRLCLLSRIFTEREMKCLDAAEAEPILDEREGVDEYNEMPMPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJI1</accession>
    <name>A0A6I9YJI1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spliceosome-associated protein CWC15 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CWC15</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC15 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924402.1">
        <property type="nucleotide sequence ID" value="XM_014068927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJI1"/>
    <dbReference type="GeneID" id="106550936"/>
    <dbReference type="KEGG" id="tsr:106550936"/>
    <dbReference type="CTD" id="51503"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013924402.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="26391" checksum="7A770870364BB899" modified="2020-10-07" version="1">RPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEEKERVAVREKNRDRPAREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEEEDEDYEEESDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPLQPQINFKVKRRWDDDVVFKNCAKGVDEMKKDKRFVNDTLRSEFHKKFMEKYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK73</accession>
    <name>A0A6I9YK73_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein HEXIM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HEXIM1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HEXIM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924637.1">
        <property type="nucleotide sequence ID" value="XM_014069162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YK73"/>
    <dbReference type="GeneID" id="106551137"/>
    <dbReference type="KEGG" id="tsr:106551137"/>
    <dbReference type="CTD" id="10614"/>
    <dbReference type="OrthoDB" id="10058500at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097322">
        <property type="term" value="F:7SK snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024872">
        <property type="entry name" value="HEXIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469">
        <property type="entry name" value="HEXAMETHYLENE BISACETAMIDE INDUCIBLE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469:SF8">
        <property type="entry name" value="HEXIM P-TEFB COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15313">
        <property type="entry name" value="HEXIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02094">
        <property type="entry name" value="HEXIMFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924637.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="32886" checksum="2BDF507127470EF1" modified="2020-10-07" version="1">MLPHFSEELTVPVVGESQSLVAPGINQIEDQAFGAAADTQDAGDGEQPDLQEQEYCGSFDQSCRSQSGGEEEKQLGKKKHRRRPSKKKKHWKPYYKLSWEEKKKFDEKQSQRASRMRAEMFAKGQPVAPYNTTQFLMEDHDQKEPDLKTSLYPKRSATKSDETSEEEFVEEEDGGSDGMGGDGTEFLQKDFSETYERYHVESLQNMSKQELIKEYLELEKCLSKMEEENNRLQMENKKFSGDSTEDPRVSQLVEELDKLRAENQKLLKEKEPGREQENNFSKLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKX0</accession>
    <name>A0A6I9YKX0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calglandulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MYL1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the cellular control mechanism of the secretion of toxins from the gland into the venom.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924420.1">
        <property type="nucleotide sequence ID" value="XM_014068945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKX0"/>
    <dbReference type="GeneID" id="106550957"/>
    <dbReference type="KEGG" id="tsr:106550957"/>
    <dbReference type="CTD" id="4632"/>
    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043292">
        <property type="term" value="C:contractile muscle fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000019">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000056">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF3">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1_3, SKELETAL MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="4" id="KW-0518">Myosin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924420.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="16825" checksum="CB2DA475E556C12F" modified="2020-10-07" version="1">MQSFSADQIADFKEAFLLFDRTGESKITLSQVGDVIRALGQNPTNSEIKKILGNPSNEEMNAKKIGFEEFLPMLQAAANNKEQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEELMKGQEDSNGCINYEAFVKHIMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YL10</accession>
    <name>A0A6I9YL10_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acylamino-acid-releasing enzyme-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924465.1">
        <property type="nucleotide sequence ID" value="XM_014068990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YL10"/>
    <dbReference type="GeneID" id="106550997"/>
    <dbReference type="KEGG" id="tsr:106550997"/>
    <dbReference type="OrthoDB" id="416344at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000043">
        <property type="entry name" value="acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="89"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924465.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="33009" checksum="4F36B24A78F7B098" modified="2020-10-07" version="1">MVAFLPPAGKEADVNWVCLEETSPIQGISWDVHILQPSPDQDNPQYRGLDFEAILLKPTQVAEQTKLPLVVSPHGGPHSVFTTTWMLYPAVLCRMGFAVLLVNYRGSLGFGQDSVDSLPGNVGCQDVKDVQSCVEQMLQKEPLDSNRVALLGGSHGGFLSCHLIGQYPGTYKACVTRNPVVNMASMFGSTDIPDWCMTEIGLPYDQAALPVPEQWEKMLLHSPMQFVDKVQAPVLLLIGEEDRRVPPKQGLEYYHALRSRGIPTRMLQYPNDNHALSSVEAEADGFMNIALWLLQHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLE9</accession>
    <name>A0A6I9YLE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Microtubule-associated protein RP/EB family member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106551304</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924859.1">
        <property type="nucleotide sequence ID" value="XM_014069384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLE9"/>
    <dbReference type="GeneID" id="106551304"/>
    <dbReference type="KEGG" id="tsr:106551304"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000001">
        <property type="entry name" value="microtubule-associated protein RP/EB family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="13">
        <location>
            <begin position="184"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013924859.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="30054" checksum="77599E656C3C915C" modified="2020-10-07" version="1">MAVNVYSTSVTSDNLSRHDMLAWINESLQLTLTKIEQLCSGAAYCQFMDMLFPGSVTLKKVKFQAKLEHEYIQNYKVLQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKDYDPVAARQGQESCPVPTLVVPMLNKPKKNLGSGGTAPTRPMVAQRTTVANKPGPPMIKKAPGGIGEDESAELIQQINALKMTVEDLEKERDFYFGKLRNIELICQENEGESDPVLQRIVEILYATDEGFVIPDEGAPPEDQEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLS3</accession>
    <name>A0A6I9YLS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphatidylcholine-sterol acyltransferase</fullName>
            <ecNumber evidence="13">2.3.1.43</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Lecithin-cholesterol acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Phospholipid-cholesterol acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LCAT</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a sterol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a sterol ester + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:21204"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:35915"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="2.3.1.43"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.43" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013925328.1">
        <property type="nucleotide sequence ID" value="XM_014069853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLS3"/>
    <dbReference type="GeneID" id="106551707"/>
    <dbReference type="KEGG" id="tsr:106551707"/>
    <dbReference type="CTD" id="3931"/>
    <dbReference type="OrthoDB" id="190846at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004607">
        <property type="term" value="F:phosphatidylcholine-sterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000090">
        <property type="entry name" value="Phosphatidylcholine-sterol acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000183">
        <property type="entry name" value="Phosphatidylcholine-sterol acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="9" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026748355" description="Phosphatidylcholine-sterol acyltransferase" evidence="18">
        <location>
            <begin position="22"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013925328.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="49678" checksum="D2763C6F04AB99A5" modified="2020-10-07" version="1">MQSQAGWSAALVLALLQPGRAFWLLNVLFPPSTTPEAAGSNRTPPVLLVPGYLGNQLEAKLDKPDVVNWMCYRKTEDYFTIWLNLNMFLPFGVDCWIDNTRVVFNKTTRSITNAPGVHIRVPGFGQTFSVEYLDGGKLAGYMHTLVQHLVNNGYVRDQSVRAAPYDWRLGPTDQEVYYLKLRGLIEEMFEAHQKPVFLIGHSLGNLHILYFLVQQPQAWKDRFVQGFISLGAPWGGAVKPMRVMASGDHQGIPIMSSIKLREEKRISTNSPWLFPTHTAWPETHIFISTPFYNYTYRDYQRFFNDINFEDGWFMWNDSKELLAGLPPPGVEVYCLFGTGHPTPETYIYNDQFPYEDPVEIIYGDGDDMISWRSIELCKEWRKQQHQKVHVLELPGAEHLNMIFDNRTLQYISEILFGNLDNVTATQDLGGRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMY3</accession>
    <name>A0A6I9YMY3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106552117</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925778.1">
        <property type="nucleotide sequence ID" value="XM_014070303.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106552117"/>
    <dbReference type="KEGG" id="tsr:106552117"/>
    <dbReference type="OrthoDB" id="9018846at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000638">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000087">
        <property type="entry name" value="Zinc finger protein 354B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001343">
        <property type="entry name" value="Zinc finger protein 568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000852">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="13">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="201"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="564"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="592"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="620"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="676"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="704"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925778.1"/>
        </source>
    </evidence>
    <sequence length="731" mass="83458" checksum="01F971C4859D35BC" modified="2020-10-07" version="1">MIGGGDKRGPRVSLIGTAETCIEKKFLKGETTNWIIQNPSFKGFSYQDSKGPRELCSQLYQIHREWLRPEKNTKAQMLDLVILEQFLAILPPEMETWVRECGAETSSQAVALAEGFLLSTANDHKQGEQQVQKPFLLAAEDTEAWRDLLYSSQELSIQEVSQEGPSENLAVSKNKTLITLVGPSLFCGVEAAVVLPTRNSLSFDDVAVFFSLEEWDLLDFNQKILYNEVMSENARNMASTGVGWPESANYKQTSVEHAQTVRNNLGEEMFENPRGPETSECNQLHKRRAKSCTPQWAEIHFYLSQQGHKKERKCPTCGKTRRENSDLCKYCRTHDKAKKYKFGECDQNYNWSLLPTLHQMTQTVEKTYPCTECGKTFSKSSNLNAHKRIHTGEKLYQCMECGKMFRWSSGLTYHKRIHSGEKPYKCTECGKSFSGLSSFTSHRRMHSGEKPYKCTECGKCFSEFSSFTSHKRMHSGEKPYKCAECGKCFSCLSSFTSHKRIHSGEKPYKCRECGKNFTISSHLISHKRIHSGEKPYQCTECGKSFTLSSHLTSHKRIHSGEKPYKCVDCGKKFRWSCHLTSHKRMHLGEKPYKCTECGTSFINLSSFTCHKRIHSGEKPYQCTQCGKNFRSATNLNEHKRIHSGEKPYKCAECGKSFRWSSCLTSHRRIHTGEKPYKCTECGKDFRRSCSLTTHKRIHTGEKPYQCTECGKSFTTNXGLQLPEFPPTEILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN02</accession>
    <name>A0A6I9YN02_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106551546</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925140.1">
        <property type="nucleotide sequence ID" value="XM_014069665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN02"/>
    <dbReference type="GeneID" id="106551546"/>
    <dbReference type="KEGG" id="tsr:106551546"/>
    <dbReference type="OrthoDB" id="9046649at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008373">
        <property type="term" value="F:sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF12">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="18">
        <location>
            <begin position="550"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="761"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="844"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="809"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="849"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013925140.1"/>
        </source>
    </evidence>
    <sequence length="926" mass="103554" checksum="5CFADACDCB89739F" modified="2020-10-07" version="1">MFSLIWKELGQCCNASERLVVTKENTPLGSIINLDGDIYSNITADAELRSVLLKLFKLFFKTPVLAYPQLLEATRPPNELFKEFLLSSRFKNLLGYETDFINALRAYPNALFLIPAISFNYHLKVGYHAIRIMKDVGLAHKAFFLHPHYLSALDRYWKLKRMRELRLSTGFMFTSFALEFCDDITLYGFWPFFYDLNGKPISHHYYDNVLPLPFVHNMSEEFSHYLKMSAQGVLHLHLGAAWAWGRQEASAFVAVKHLLISNDVLVQYSEHLLLVLACDASPYGVGTVLCHQLPSGAEVPVAFFSHTLSASERNYAQIDKEALAIVARVRRFHHYLYGRPFVIVTDHKPLLGLLAGDRPAPQVLSPRMTHWFVFLASYEYQLNHRLGKQIAHADALSRCPLPEAPEDPAAVAPVFLIDNLNLPTSAADIAWLSARDQVISQVLDWVRRGWPKYPVDPVFLPFKSRLAELSVQRGCLLWGHQVVVPASLRTTVLQQLHHAHPGIVRMKALGRSYVWWPKLDQDIAAHVAGCPKCQSAQALPPKAKPRTWEPPSSPWSWVHVDFAGPIQGRMLFIAVDAFSKWVEVVHMPSTTADALVATLHHLFATHGLPDVLVSDNGPRIQHALISPSHPASNGLAERAVRSVKEALAKMDHLPWQQRIDDFLLAHHSTPSPESNTSPAELLMGRRLRTTLDCLHPAYATPTLLDSRGGTRSFGQGEAVYARNYGGHPLWLPGIVVDQTGPCSYRVRLNDGRTWRCHCDQLRGRHQPPTNLQPPTDPQLRASDAGPRSEEAARQHTTCAPNRSGDLPGSGSTTAELGDATATSCRADTSRQPTCSHQQIHTFEQATRGPEARKLRDNRQPVPQTEAATYLVGWHRPWLVDDLSDRHPVKKGPRQGIQILQAAPKGLACPAKSGGGQRIYKTTHARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN94</accession>
    <name>A0A6I9YN94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TRIM56</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013925379.1">
        <property type="nucleotide sequence ID" value="XM_014069904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN94"/>
    <dbReference type="GeneID" id="106551756"/>
    <dbReference type="KEGG" id="tsr:106551756"/>
    <dbReference type="CTD" id="81844"/>
    <dbReference type="OrthoDB" id="264520at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060340">
        <property type="term" value="P:positive regulation of type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001287">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF299">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="157"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="372"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013925379.1"/>
        </source>
    </evidence>
    <sequence length="687" mass="74927" checksum="F58EA40106AD41F2" modified="2020-10-07" version="1">MAADASSLWETLAGSFLTCNICLGHYCQPKILPCLHSYCQDCLKTLQGGAKRELRCPECREPVPLPAGIEGLKTNFFINGLLDLVPPVEKARVTCSLCPLIGQDASSVAVSHCIDCADHLCQACASGHRCSRLTHKHLVVDMEAYHSGKYNEEIRKRQAARCKEHKGENLQYFCIPCAAALCRECRLGHHLEHPCLSLSEAAKARRPVITGLLAGVAEKVQLIARGKASLEEEIKEMEVWNTNIRSVVEQTCAKAVEQLLSHKGQVLAQLSSYLEERKVASRLLLSELGFQEQVASSTVAFAQKVLSLGQEEEIVSLEQMISERLRQLQGFSWEALAVQVPKLRIQPNLLCGCNLFHLEFHEKPTAAAPEGYAQVARKKAKQKPPQGAEVGEACRQEQAPSGGSSGLLESGGPPSPNGPEVPRLTPKPVFFCSFWVKVPTDKKQPQITGLCPFGSGEILLADEQNKKLKRFSVQGEFKGTIPVPGDVAPFSVAAVGNKVVFTAASQLYVLNETGGIVWQKALKGGQASHAVTTCDGNCVVVSVAGHLEVFNMKGQLLERIVPEGSHDRSLVFLGRWKEGFVASDWYRRSVVLLGRKGDLVTEYQEEQLKESQPGSVCADAPGIIYVVLRELNKVVAFSEAGEVLGSFLTTENNIFKPRMVTIAGDGHFVAALTNGTVHVFKIKYQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNZ8</accession>
    <name>A0A6I9YNZ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Properdin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106552294</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926007.1">
        <property type="nucleotide sequence ID" value="XM_014070532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNZ8"/>
    <dbReference type="GeneID" id="106552294"/>
    <dbReference type="KEGG" id="tsr:106552294"/>
    <dbReference type="OrthoDB" id="446173at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049536">
        <property type="entry name" value="CFP_TSR-0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054019">
        <property type="entry name" value="CFP_TSR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052065">
        <property type="entry name" value="Compl_asym_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906:SF43">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22195">
        <property type="entry name" value="TSP1_CFP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18487">
        <property type="entry name" value="TSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026666024" evidence="7">
        <location>
            <begin position="29"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013926007.1"/>
        </source>
    </evidence>
    <sequence length="418" mass="46731" checksum="E745C05C425CAC03" modified="2020-10-07" version="1">MKTLGRKSLQFLVIVCWFFASLESPAEAENVLCYKTFDEISGTCNNLLGDEVAQEDCCLNHEYGFQSTEQGPCQSCREAKWSQWTSWSACSVSCTEGVQRRSRVCYGLPSEPMECPSGRREREMKSCLLKECCPIFFHVFLFNAAHGNWGNWGPWQHCSHTCSVEGSASQPQQQRTRPCNNPPPSSSPPGNPCPGASRESQTCTGLPFCPRDGNWGSWKPSQPCSVTCGVGQVVQNRQCDNPPPKYGGRACLGAAERRQPCTIKVPCPVNGHWSEWGAWQECTRHEIGFEIKCEEIPAIQRRTRKCIGYRNGGSRCSLSGRESRVCYHFDGCSVTGQWTTWSSWGLCEPACGPEPKKSRKRVCQAIYPNFPKVVVGENQKEQNVTFWGKPKPQCKQLEGQSLEVVEETPCRNVPPCED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPP1</accession>
    <name>A0A6I9YPP1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106551997</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">F-actin regulator involved in anterograde Golgi to endosome transport: upon ubiquitination via 'Lys-33'-linked ubiquitin chains by the BCR(KLHL20) E3 ubiquitin ligase complex, interacts with EPS15 and localizes to the trans-Golgi network, where it promotes actin polymerization, thereby facilitating post-Golgi trafficking. May play a role in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925655.1">
        <property type="nucleotide sequence ID" value="XM_014070180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPP1"/>
    <dbReference type="GeneID" id="106551997"/>
    <dbReference type="KEGG" id="tsr:106551997"/>
    <dbReference type="OrthoDB" id="1850764at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000076">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF20">
        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="235"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="358"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="401"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="163"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="626"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013925655.1"/>
        </source>
    </evidence>
    <sequence length="693" mass="75834" checksum="AD821C6425A8BD21" modified="2020-10-07" version="1">MMEREVCLWDSRRLNCSLTSVTLDASPKPLIPLFDLSSGLLVLAGKGEKGLFCYEVQPAQPAVVEVHQSFLETRTQGAAQVPRLALDVTACEVMRVLQLSDSAVVPISYLVPRKATHEFHKDLFPDCPGNVPAASAQAWWAGDNTQVELVSLDPAHRASPGFLSPCLPRLDQDSQHPPSAHVEAGDQAKHPDHSVGSSLSSPGSSLASPSSVAPSLPATSGFSSSPSQRSLQNILGPSSAFRHIEGVVLPRTTHITNLRGLSLTTPGECDGFCANQHRIAIPLLATGGQVAILELSQPGRLPDTPMPTIQNGAPISDLCWDPFDEQRLAIAGEDTKIRLWRVEPTGLQKQLLEPEVVLRGHTEKIYSIKFHPLASDVLASSSYDKSVRIWDVRTGRQELGLQGHTDQIFSLAWSPNGRCLATVSKDGRVRVYEPRQAAHPLQDGPGPKGGRGARVTWVCGGQHLLVSGFDSRSERQLSLHQAEALAAGPLATIGLDVSPSTLIPFYDVDTSVAFFTGKGDTRVFVYEVVPEAPFFLECNSFLSCDPHKGFHFLPKTACDVREVEIARALRLRQSSIEPVAFRVPRVKKEFFQDDLFPATQVWWEPALPATAWLKGANKQHRITSLQPKDMTPVSQAPKETPKRKYLPSSVYLEEKSDEQKKEELLSAMVAKLGNRDDPLPQDSFEGVDEEEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRB7</accession>
    <name>A0A6I9YRB7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calcium-activated potassium channel subunit beta-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">KCNMB4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926180.1">
        <property type="nucleotide sequence ID" value="XM_014070705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRB7"/>
    <dbReference type="GeneID" id="106552434"/>
    <dbReference type="KEGG" id="tsr:106552434"/>
    <dbReference type="CTD" id="27345"/>
    <dbReference type="OrthoDB" id="9932001at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015269">
        <property type="term" value="F:calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005513">
        <property type="term" value="P:detection of calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003930">
        <property type="entry name" value="K_chnl_Ca-activ_BK_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258:SF3">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03185">
        <property type="entry name" value="CaKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013926180.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="23929" checksum="FE2244D15CC7F607" modified="2020-10-07" version="1">MAKIRVAYEYTEAEDKTIRLGLFLIVSGILSLFILGFCWLNPALQDLQGKAANCTVLSVQRISEVFECTFTCGADCKGTSLYPCLQIYVNNSESNARALLHPDEHQLLTNPKCSYIPPCERENEKNSDIVMHWQIYWEDEVGSQPFTCYFNQHLRPDDVLLQRTHDESVLLHCFLWPVVTFLVGVLIVILTICAKSLAVKAETMKKRKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRI6</accession>
    <name>A0A6I9YRI6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC106553028</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the dynein light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926922.1">
        <property type="nucleotide sequence ID" value="XM_014071447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRI6"/>
    <dbReference type="SMR" id="A0A6I9YRI6"/>
    <dbReference type="GeneID" id="106553028"/>
    <dbReference type="KEGG" id="tsr:106553028"/>
    <dbReference type="OrthoDB" id="10033309at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21452">
        <property type="entry name" value="DLC-like_DYNLL1_DYNLL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000001">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF91">
        <property type="entry name" value="DYNEIN LIGHT CHAIN 1, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 24" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="22 24" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17 24" id="KW-0243">Dynein</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 24" id="KW-0493">Microtubule</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21 24" id="KW-0505">Motor protein</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013926922.1"/>
        </source>
    </evidence>
    <sequence length="89" mass="10350" checksum="15F6656D183BEB27" modified="2020-10-07" version="1">MSDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSF7</accession>
    <name>A0A6I9YSF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553279</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013927222.1">
        <property type="nucleotide sequence ID" value="XM_014071747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSF7"/>
    <dbReference type="GeneID" id="106553279"/>
    <dbReference type="KEGG" id="tsr:106553279"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000101">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927222.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="17104" checksum="FD82D6DF12F69044" modified="2020-10-07" version="1">MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKGKQESFYCTWIYISRDNMQMSLLFNFLLAISSEFKALRPVYTKVYGVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YST8</accession>
    <name>A0A6I9YST8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 180</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CCDC180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926700.1">
        <property type="nucleotide sequence ID" value="XM_014071225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YST8"/>
    <dbReference type="GeneID" id="106552845"/>
    <dbReference type="KEGG" id="tsr:106552845"/>
    <dbReference type="CTD" id="100499483"/>
    <dbReference type="OrthoDB" id="431588at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028089">
        <property type="entry name" value="DUF4455"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444:SF14">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14643">
        <property type="entry name" value="DUF4455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4455" evidence="2">
        <location>
            <begin position="58"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926700.1"/>
        </source>
    </evidence>
    <sequence length="556" mass="65485" checksum="BE995AEEA1D56A15" modified="2020-10-07" version="1">MPNDDLTENPVQYKKAVTLAHAKAKESESIIAAREVRGLADVIVSEKRTSNVVERIYENKKLCYEEYVAQFRHELACIGKEIEEYYVKPGKLLLTQLQESDQSFESYFRASESDITLISYAIEDFEKMWVLLFQESQQRRQWIRELDEALCQAEVTRAERITVVLSKYTKMLEDISYVLSSDVHRFIHKEAMMINQALLANRRAISKLFLNLMEADLKRDSSYRYQLEERKEVWKGVQKKHLISSFREFMESERIQNPISVQMELESMLEDQRILAQRRNEHLFSLGSLLPLEHTKAEINAWYESLVDLNKQIDTHNVQSMMRIRLNYEKVCQECLEKLQECKQKLIDLKICTHKEAEKIVNPNFYQLIGMLQVQFEAKIEKMDAELEQLAKNSEINCRHLYQYFQDVLAIFDVHHLKLTNQESDLQKALKDCRSKHENMNKLREMNLDIAVDKLRMQSTDEKLKACLEKVYFALDIIGNGYSIFYQDLVSKVITYPRRVRHELLFYSAAISRYFYVLGSYKGKTLKKPESSTEGKREEGEITQESQEIEETEEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSX7</accession>
    <name>A0A6I9YSX7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zonadhesin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106553142</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927059.1">
        <property type="nucleotide sequence ID" value="XM_014071584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSX7"/>
    <dbReference type="GeneID" id="106553142"/>
    <dbReference type="KEGG" id="tsr:106553142"/>
    <dbReference type="OrthoDB" id="5945029at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052749">
        <property type="entry name" value="Alpha-tectorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160">
        <property type="entry name" value="ALPHA-TECTORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160:SF8">
        <property type="entry name" value="VWFD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="726"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="13">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="13">
        <location>
            <begin position="250"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="652"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="692"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="701"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927059.1"/>
        </source>
    </evidence>
    <sequence length="778" mass="85192" checksum="66101086D0610C8C" modified="2020-10-07" version="1">MCGNFNNQTADDYLMSDGHLAVNDSQLGNSWKAPGDSDVGCQPDSRPDLNPSCSAQELERLSALCLEIMAPKYQACHALINPELFFHNCLYDMCQYQGLSSLLCDNLQAYVEACKSYGVTGISWRNSTFCPLPCPPHSHFSECASPCPATCSNLYAPASCQYPTPCVEGCTCDRGYVLSDLACVPMRECGCRDPQQEYHNSGDTWVTSGCQQRCKCLSGQIECQTFGCPTESQCTTSNQGLHYCRPISFHQCVISGDPHYRTFDNFVHHFQGRSTYTLTRTMGRVPDGLEGLSVDGRNHRRSIFHRFSFLREVYVEVLGFRVTLMQGRKMAVNGVRVTPPYSPTEGLQITQRGRRLIVETNFGFSVSYDGHNSAEIVLPSTYKERVGGLCGNYDGRRNNEYIKPDGTRARTLNAFGNSWQVSVKSKELDGGSWTLGRARARRQEPEEEEPESGFEMDCTPDQLAFVNGTRACGALSDPSGPFSACHARVSPGPFHESCVYDLCALFNDTELLCQALEAYAQLCREQEVDLGPWREKMGCSVECPAHTSYQPCMTACPTSCANLAAQSSCEAPCVEGCASNPGYVLSGLVSVPYGQCGCSSNGQYYQINETFVTEDCSKRCTCRATDLLECFPVGCGPGEICAVSDFIRGCFRDNACVSNPCQNEGKCQVTPEGFKCACVEGYIGPLCEDSPFESSTPAPPKTTTLSTTRTLPDVSQTTGSPPGPDLLTILLSVFIPLAVILLLTLIVLCVCRHRNHGYQMKLEEDHVSIISTTGFPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTS2</accession>
    <name>A0A6I9YTS2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106553902</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; sulfate assimilation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927963.1">
        <property type="nucleotide sequence ID" value="XM_014072488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTS2"/>
    <dbReference type="GeneID" id="106553902"/>
    <dbReference type="KEGG" id="tsr:106553902"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050428">
        <property type="term" value="P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000001">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000006">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF17">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="10">
        <location>
            <begin position="131"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927963.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="41601" checksum="D137B0CB87A895EA" modified="2020-10-07" version="1">MQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLAVTEEDKERLDGCTAFALLYDNRRVAIVRNPEFYEHRKEERCARQWGTTCKEHPYIKMVMEQGDWLVGGDLQVLDRIYWNDGLDQYRLTPADLKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLDRGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGILNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYDPTHGAKVLTMAPGLITLEIVPFRVAAYNKKKKCMDYYDSEHHEDFEFISGTRMRKLAREGQNPPEGFMAPKAWTVLTEYYQSLEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTU1</accession>
    <name>A0A6I9YTU1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106553149</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927065.1">
        <property type="nucleotide sequence ID" value="XM_014071590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTU1"/>
    <dbReference type="GeneID" id="106553149"/>
    <dbReference type="KEGG" id="tsr:106553149"/>
    <dbReference type="OrthoDB" id="9038399at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026704012" evidence="13">
        <location>
            <begin position="20"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="223"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013927065.1"/>
        </source>
    </evidence>
    <sequence length="491" mass="54803" checksum="20B4725E8F08B19A" modified="2020-10-07" version="1">MVWFLLLLMLLFLLPQATCRILSAKCLLTMGRDAVEPFNHYKPGLHLISGLFSATNLAKIQPFSFNEINKDPQLLPNITLGYNLQDNFRNRRMTLDGLLDLLSNGEANVPNYSCGRKKDMLVLLEEADSDISIHIAALLGIYKIAQPWPYSRCVEACQPGYFKVVQEGKPSCCYDCIPCAEGSISTQEDADHCSKCPEDQHQNKNRDQCIPKIITFLSYQENLGVILTSFALFLILTSSFVLGIFSKYRETPIIKANNQDLSYVLLVSLLLSFCTSFLFIGRPRRATCLLRQTVFSIIFSVAVSSVLAKTIMVVLAFLATKPGNRVRRWLGKSLANSILLSCSSIQVILSSIWLGVFPPFPDSDMHSQSGEIILQCNEGSTVMFYIALTYMGFLATICFMVAFLARNLPGAFNEAKLITFSMLVFCSVWISFVPTYLSTKGKYMVAVQVFSILASSAGLLGCIFLPKCYIILLRPDLNTKEKLILKNEMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU83</accession>
    <name>A0A6I9YU83_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interferon-inducible GTPase 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106553255</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927200.1">
        <property type="nucleotide sequence ID" value="XM_014071725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU83"/>
    <dbReference type="GeneID" id="106553255"/>
    <dbReference type="KEGG" id="tsr:106553255"/>
    <dbReference type="OrthoDB" id="422720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF10">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="14"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="241"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013927200.1"/>
        </source>
    </evidence>
    <sequence length="598" mass="66315" checksum="96B09BB2AE08DCA4" modified="2020-10-07" version="1">MTSPNSAAGSPDCGDVSIIFVGKSNVGKSALLNAVRDMTEGDVGSAPVGAPVKSEKPIMYPDPIYNNLLLWERSMEGEGHADQWMREADLIVLVTDDRFEDSHARLVLEARQAGKKVYFARSKADLELHTLKRLMGQRYDRSEVLRALREACTESLGTIPLVDPFVYLISAFEPGALDCPQLREDLLKDLHQYERLQKPTRLSFEEFSEKEIAEIQEAYELGGLSEVVTRIQCSLETIWNAQLDIAITGESGAGKSTFVNVLRGMTDEEEGAAATGVTETTMQPTAYPYPGHANVVLWDLPGIGTPNFLADKYLETVEFSRYDFFIILASERFKENHVRLAQAIVQQGKQFYFVRTKIDNDLEAEQRKKNPPAEAEVLEVIKKDCQSKLSKAGLSDAKVFLLSNFYLDKFDFQAFEETLEQELPAHKQQAFLLSLPNVSTVIIEKKKKVLLQEIWKVALISSLVAAVPLPGLAFACDVSILLKKLSSYRQDFGLDAASLSRLAERSGKSMEVLRKEIRSCLGRSINKDVVISLLGKATGTGLMVANLFLHRFPIYGALASGGISFHTTCSMLRQCLEELADDSQRVLMKACESHAGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV13</accession>
    <name>A0A6I9YV13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alpha-mannosidase</fullName>
            <ecNumber evidence="17">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MAN2A2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 H2O = 2 alpha-D-mannopyranose + an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:56052"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28729"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="3.2.1.114"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013927846.1">
        <property type="nucleotide sequence ID" value="XM_014072371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV13"/>
    <dbReference type="GeneID" id="106553803"/>
    <dbReference type="KEGG" id="tsr:106553803"/>
    <dbReference type="CTD" id="4122"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004572">
        <property type="term" value="F:mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000009">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF57">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 17" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="19">
        <location>
            <begin position="502"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013927846.1"/>
        </source>
    </evidence>
    <sequence length="1151" mass="130188" checksum="81261F5D5EBCA7D2" modified="2020-10-07" version="1">MKLKKQVTVCGAAVFCVAVFSLYLMLDHVQRDPARPLPGGHLPRSQISALQSRIEQLEQLLEENHEIIGRIKDSVLELTVGHEGRSALLPLRLANGSWALPADGRPGFFSVAPGDCRLGPRAARPEPALQMLAVSALLPYDNQDGGAWKQGFEITYEPHEWDAQPLQVFVVPHSHNDPGWIKTFDKYYVDQTQHILNNMVLKLQEDPRRRFIWSEISFFSKWWDNISSQKQTAVRRLIGNGQLEMATGGWVMPDEANSHYFAMIDQLIEGHQWLEKNIGVTPRSGWAVDPFGHSATMPYLLRRANLTSMLIQRVHYAIKKHFAATQNLEFIWRQSWDLDSSTDLLCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKAITSSNVAERAQLLLDQYRKKSKLFRSSVLLVPLGDDFRYDKPQEWDAQFLNYQRLFDFLNARPELHVQAQFGTLSDYFDALYKQMGIVPGMRPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSMGRVLEAHLRAAETLYSLALSHARHAGMESRYPLSDYAMLTDARRNLGLFQHHDAITGTAKEAVVVDYGVRLLHSLMNAKRVIINAAHYLVLADKESYHYDMAMPFLGTDETRLNQDSLPEKMVIKLDATPRFVVVFNPLEQERLTVVTLLVNTPRICVLNEEGESLAVQLSAQWESATDMVPDVFQVSVMIRLPALGLTILQLIKSFDNHNTLKSSVRLFLHGHDFAGHKYEAVPVQVLSTATDDFCLANQHMRACFSGPSGSLKSVHHIGEKQEQQLSSQFLVYGTRTTKDKSGAYLFLPDGEAKPYTTKDPPVVRVTEGPFFSEAAIYYQHIQEVVRLYNVPGVDGLSLEISCLVDIRDHVNRELALRFSSSIESKGRFFTDLNGFQIQPRKYLKKLPLQANFYPMPVMAYIQDSQHRLTLHTAQALGVSSLSSGQLEVILDRRLMQDDNRGLGQGLKDNKQTCNRFRLLLERFSSANKAQENQLTSFPSLLSHITSMHLNAEVLTMPLVQEKLPPPALRSFAPFSITVPCDFHLLNLRTLQGEDEALPSAETALILHRKGFDCALEARNLGFNCTTTQGVLSLGSLFQSLNLISLQPSSLTLMYPLTTASLNSTTIHLDPMEIATFRLRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVU5</accession>
    <name>A0A6I9YVU5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein myomaker</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TMEM8C</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928144.1">
        <property type="nucleotide sequence ID" value="XM_014072669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVU5"/>
    <dbReference type="GeneID" id="106554072"/>
    <dbReference type="KEGG" id="tsr:106554072"/>
    <dbReference type="CTD" id="389827"/>
    <dbReference type="OrthoDB" id="8770806at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021910">
        <property type="entry name" value="NGX6/PGAP6/MYMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319">
        <property type="entry name" value="FIVE-SPAN TRANSMEMBRANE PROTEIN M83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319:SF7">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12036">
        <property type="entry name" value="DUF3522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013928144.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24722" checksum="1B58B355D3E7DF02" modified="2020-10-07" version="1">MGSLAAKFLLPTVSSLAFLPTVSIAAKRQFHMEAMVYFFTMFFLLVCHACEGPGLIFLCFMKYELLQNFSIFGTAFSMWVSLIALAEFDEPKRSTLVMFGVLTIAVRIYHDRWGYGVYSGPIGTAVLAITVKWLQKMKEKKGLYPDKSVYTQQVGPGFCFGALALMLRFFFEDWDYTYVHSFYHCALAMSFVLLLPKVNKKAGNGGAPAKLDCSTLCCYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWC1</accession>
    <name>A0A6I9YWC1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18 19">Cationic amino acid transporter 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">LOC106554457</name>
    </gene>
    <organism evidence="16 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928595.1">
        <property type="nucleotide sequence ID" value="XM_014073120.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928596.1">
        <property type="nucleotide sequence ID" value="XM_014073121.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928597.1">
        <property type="nucleotide sequence ID" value="XM_014073122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWC1"/>
    <dbReference type="GeneID" id="106554457"/>
    <dbReference type="KEGG" id="tsr:106554457"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000009">
        <property type="entry name" value="Low affinity cationic amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004755">
        <property type="entry name" value="Cat_AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00906">
        <property type="entry name" value="2A0303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF20">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <end position="265"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="407"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="491"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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        <location>
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            <end position="599"/>
        </location>
    </feature>
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            <begin position="554"/>
            <end position="604"/>
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    <evidence type="ECO:0000313" key="19">
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            <dbReference type="RefSeq" id="XP_013928597.1"/>
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    </evidence>
    <sequence length="634" mass="68805" checksum="05E7D45352153993" modified="2020-10-07" version="1">MLKKTLTNFGKKLIRQRTVNLGSQETQLARCLSTVDLIALGVGSTLGAGVYVLSGEVAKDLAGPSIVICFFIAAVSSVLAGLCYAEFGARVPKAGSAYLYSYVTVGEIWAFTTGWNLILSYMIGTASVARAWSSTFDHIIGGHISTFFRNHTALHLENVLAEYPDFFALFLVLLLTGLLSFGVSESALVNKIFTAINLLVLSFVIVAGFVKGDVKNWNLSAEDCQNRTETLLESGQKVRFGNGGFFPFGFQGVLAGAATCFYAFVGFDCIATTGEEARNPQRSIPISIIISLIICFVAYFGVSASLTLMVPYFLVDKESPLPDAFKVVGWEPARYVVAIGSLCALSTSLLGSMFPMPRVIYAMAEDGLLFRFLFRVHARTKTPLVATVVSGIIAALMAFLFELKDLVNLMSIGTLLAYSLVAICVLILRYQPELFRFSKDLEMLEINGSEEEKMTINSTVGDGQNAFKEKFSCRSLFFPAEDNPTHVSGRIVYISTTAVAVTITALCGILAHEWEALLEGSIGWVIACVILLGILLAGTAVIWRQPESKTRLTFKVPGLPLLPLFSILVNIYLMMQLDAGTWARFAVWMAIGFAIYFGYGLQHSEEGQGAQQSPSAADKGLPSAHSELSQELAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWU7</accession>
    <name>A0A6I9YWU7_9SAUR</name>
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        <submittedName>
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        <name evidence="3" type="primary">LOC106554380</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <location>
            <begin position="268"/>
            <end position="413"/>
        </location>
    </feature>
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        <location>
            <begin position="276"/>
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            <begin position="392"/>
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    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="413"/>
        </location>
    </feature>
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    </evidence>
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    <sequence length="413" mass="46195" checksum="985454779A1078B5" modified="2020-10-07" version="1">MDSFDLALLQEWDLDSLCVYDLDRNMLRRKEWERRNQETQHEDSIFNTSYSLFSEPYKTNKGDELSSRIQNTLGNYDEMKELLTDHSNQSQLVGVPKACMSQASMNKMEEQFVAETMTHTQTSVCSPQSSLPVTLSGQLSKNATIGWQKSGRCLENQQRGKHRHGLSDSQSNNFKIANQKSSLEKIIHYVHTPSPSSSCSAIQGPVASLLVENIQTQQQSKLSCATETGVQAQERPAMHSNGHCVQNFHPALASKPNIIQQKPTAYVRPMDGQDQAPDKSPRLKVPDETSTLCTSYREMSSVKNESARTKSKMKKCDISKQEEDSGVGDDDCVEEILRDSQHVSTAHSNQRKDDVEPKNSEDCTLHTSMLEDDLKLSSDEEESDQQAAQKSTLRVLSDSSPANQQSSCRILGV</sequence>
</entry>
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    <accession>A0A6I9YX96</accession>
    <name>A0A6I9YX96_9SAUR</name>
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        <submittedName>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
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    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF57">
        <property type="entry name" value="ZINC FINGER PROTEIN 154-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
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    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="71"/>
            <end position="98"/>
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    </feature>
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            <begin position="99"/>
            <end position="126"/>
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            <end position="155"/>
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            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
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        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
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            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
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        <location>
            <begin position="734"/>
            <end position="761"/>
        </location>
    </feature>
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            <end position="449"/>
        </location>
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        <location>
            <begin position="825"/>
            <end position="886"/>
        </location>
    </feature>
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        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
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        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
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        <location>
            <begin position="842"/>
            <end position="858"/>
        </location>
    </feature>
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        <location>
            <begin position="860"/>
            <end position="872"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013928576.1"/>
        </source>
    </evidence>
    <sequence length="1000" mass="111082" checksum="7A7A52D7BCC0281F" modified="2020-10-07" version="1">MYMKEGTLPAGNLPPSLEDVPTFRCEDCDELFQSKMEMRRHKKYSCHAVGSLYESLSEEIKQEGMEDDQGHECKDCERMFPNKYSLEQHMIIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYNPGGLFAPGLPLTPSSLMDKSKTSPSLNHSGLGFGDYFPARPHPGAIPFSPAPPSFPALAPGFPGIFPPSLYPRPPLLPPAPLLKSPINHTQEAKLPSPLGHSPLPLMSAIGNSGHPLHVEERFNSAPLGNSYLEKLKARTSDLSDASDFEDINTTTGTDLETTTGTGSDLESDAESEREKTKEKSKPGESKADFGSGSAPTGSANSTSDLPIFYSQHSFFPPPEDQLLPMMGATNDSIKAIASIAEKYFGPGFMGMQEKKMGALPYHSMFPFQFLPNFSHSVYPFTERTLNHSLLVKAEPKSPRDLHKIGGACSESPFDLTTKPKESKAAHVLPARHGGEEQPLDLTISSRIRASHHGSRDARKNHVYGERKLAASDGVPKMNQAQMLPQPSLHYAKPSPFFMDPIYSRVEKRKVADSVGAIKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKTDAGGASLPPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRRVSQHAGVIANHLGNNASSPASESEHQALLDEKEDAYFSEIRNFIANSEMNQSTALAEKRPEIQNADGATHGLPNDKVEDVDEEEDELEEEDDDSLTGKSQDETVSPTAEPQGGYEDEEEEEAASLTMSFNRAQRCTEENEVGLLDLEQMSEFGKGLDFRQASEEAFDVKAVYNSTLDSEALKQTLFRQAKNQAYAMMLTLTKNTPFHPSSQNPLDAWLNMAGAASEPGTFNAVSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXG4</accession>
    <name>A0A6I9YXG4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable ATP-dependent RNA helicase DDX52</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">DDX52</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013929348.1">
        <property type="nucleotide sequence ID" value="XM_014073873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXG4"/>
    <dbReference type="GeneID" id="106555098"/>
    <dbReference type="KEGG" id="tsr:106555098"/>
    <dbReference type="CTD" id="11056"/>
    <dbReference type="OrthoDB" id="360161at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000759">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="210"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="331"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="527"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="539"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013929348.1"/>
        </source>
    </evidence>
    <sequence length="553" mass="62553" checksum="9BFDDBF2A9160915" modified="2020-10-07" version="1">MDAHELFRKLGAGAKFDVRRFRRDAERFGVLRTKEETTKTSEILGSLDFFGKKRKENELHEAQIKTLELSNLPLEEVAAPQKTQAAKKRKGDGQSTKERRKKKKGQETVAVIPGTENDGILWMSTLEAKLDIPKSKGKKGPKSQKLKQIRQEKINHFRNMHKIYIQGTDLPDPITSFEELGREYKIHSKIMQNVEAAGFQSPTPIQMQAIPVMLHGRELLACAPTGSGKTLAFSIPILTHLKQPMNKGFRALVLSPTRELASQTHRELLKLSEGIGFRIHLIHKAAETAKKFGPKSSQKFDILVTTPNRLIYLLKQDPPAIDLTRNAAVETVDQELLFVGSETGKLLAMRDLVKKGFTPPVLVFVQSIERAKELFHELVYEGINVDVIHADRTQQQRDNVVRSFRAGKIWVLICTALLARGIDFKGVNMVINFDFPSSAVEYIHRIGRTGRAGHTGKAITFFTEDDKPLLRSIASIIQRAGCPVPDYIKHFRKLQSKQKKKFTKKPLERAHILTSLKFLQKKAKKNKTLVQKMKKNSKEVQSNNSQKQATPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYQ9</accession>
    <name>A0A6I9YYQ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Golgi apparatus protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GLG1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Golgi outpost</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929838.1">
        <property type="nucleotide sequence ID" value="XM_014074363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYQ9"/>
    <dbReference type="GeneID" id="106555492"/>
    <dbReference type="KEGG" id="tsr:106555492"/>
    <dbReference type="CTD" id="2734"/>
    <dbReference type="OrthoDB" id="2015434at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001893">
        <property type="entry name" value="Cys-rich_GLG1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017873">
        <property type="entry name" value="Cys-rich_GLG1_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039728">
        <property type="entry name" value="GLG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884:SF1">
        <property type="entry name" value="GOLGI APPARATUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884">
        <property type="entry name" value="SELECTIN LIGAND RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00839">
        <property type="entry name" value="Cys_rich_FGFR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51289">
        <property type="entry name" value="GLG1_C_RICH"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0730">Sialic acid</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="915"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="24"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="160"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="371"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="443"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="681"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="810"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013929838.1"/>
        </source>
    </evidence>
    <sequence length="948" mass="108315" checksum="035AB4A34F18385C" modified="2020-10-07" version="1">MRLPLQSIAKLQLVQNKTARPDNEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTISEIKECADEPVGKGYLVSCLVEHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDQCRSDINFLKCGSIRPGEKDAHSQGEVVACLEKGLVKVAEDNEHRIKVSESCMKAILRVAELSSDDFHLDRHLYFACRDDREHFCETTQAGEGRVYKCLFNHKFEDAMSEKCRDALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRTREARLSYLLMCLESAVHRGSVHPFCSFVIPYRHLSYSGAYIIFLAQYFHSLQTLIQEVDPVADYRIDRALNEACESVIQTACKHIRAGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDSVLYRKCQGDASRLCHTHGWNETSEIIPPGAIFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRALDVKLDPALQDKCMIDLGKWCSEKTETGQELECLQDHLDDLVTECRDVVGNLTELESEDIQIEALLMRACEPIIQTFCHSCSDHLGTKKVELEEQADGGESDAWGHPPCYISFLSTFQTLFPLEKGISFFENVIEKVCSALAKVSHDGGWLHVLSLGLGGRMSLQALPSCTVSYLEEMFKHRRSFSLAELLFPCLPWQRFCPEADAKNMLQCLKQNKNSEVMDPKCKQMITKRQITQNTDYRLNPVLRKACKADIPKFCQSILSKAKEESELEGQVISCLKLKYADQRLSSDCEDQIRVIIEESALDYRLDPQLQMHCSEEIFTLCAEEAAAQEQTGQVEECLKVNLLKIKAEMCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSFAAKVAPAEGFSDLAMQVVTSPSKNYILSVISAGICVLFLIGLMCGRITKRVTRELKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0D6</accession>
    <name>A0A6I9Z0D6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Outer dense fiber protein 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106555668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ODF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930037.1">
        <property type="nucleotide sequence ID" value="XM_014074562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0D6"/>
    <dbReference type="GeneID" id="106555668"/>
    <dbReference type="KEGG" id="tsr:106555668"/>
    <dbReference type="OrthoDB" id="9948429at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902018">
        <property type="term" value="P:negative regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026099">
        <property type="entry name" value="Odf2-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23162">
        <property type="entry name" value="OUTER DENSE FIBER OF SPERM TAILS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23162:SF7">
        <property type="entry name" value="PROTEIN BCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="221"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="418"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930037.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="64975" checksum="B9750E4CA90BD727" modified="2020-10-07" version="1">MESNGEMNCMSYLASGEAENFCCIFQSCDSSFVKGHLGTESSSQLGKDLFHESIDKSDMHPAESEEVESLKENWISYNRFMNKHRSRMREPLPSLEDKPQESTLEESLNTSETKRSWEDAMKDKVAFQEMLHEAELAVNSAEMFLPSFKESLVRIAKACCISASDEITISMQENLLIKELEAIQKMKELLSWLLKSSKEKEIIGNETKDLDQTLSESKIYVESLRNEVIQQEKHILELSDQLQQEKAKIQKTYRHSEAIQTVQTHLQSQVEKKEEENNQLRAKFQTMEKNIAEWKRQVGEYKQKILAEKKRKVERRNALKRAAVVQKQRAEQMKMVVENLFSKVKEKEIQLSEAFSAPNIWKSHHKIIIEEKIKLEVQVETLKKQTTDLVMDLNRIEEDERKSTNKIIQKINFVASENKKISTENSRLKASLDDLEADFITAEAELKSLHEQVHQQEELVEQYKIEVAKLEVEAEELRTRYEKIMHDSRTITEGRDVERDAMKSQTEARQKEVEYARGLQKAAEEKLQKYQESLLSCQKSCVEKSKTLRELQAQVLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0D9</accession>
    <name>A0A6I9Z0D9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="8">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106555431</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="8"/>
    <dbReference type="RefSeq" id="XP_013929754.1">
        <property type="nucleotide sequence ID" value="XM_014074279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0D9"/>
    <dbReference type="GeneID" id="106555431"/>
    <dbReference type="KEGG" id="tsr:106555431"/>
    <dbReference type="OrthoDB" id="10257085at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000200">
        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="10">
        <location>
            <begin position="88"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="292"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013929754.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="40055" checksum="9C6C9BEFC7D8E81E" modified="2020-10-07" version="1">MAAALAAAEPGVTDGAGRGDRGDGSNHSADTLDRLLPTIVGTKSPRKRTTSQSKSEPPLLRTSKRTIYTAGRPPWYNEHGTQSKEAFVIGLGGGSASGKTTVARMIIEALDVPWVVLLSMDSFYKILTKEQQELAASNDFNFDHPDAFDFDLMIATLKKLKQGKSVKIPVYDFTSHSRKKEWKMLYGANVIIFEGIMAFADKDLLKLLDMKIFVDTDSDIRLVRRLRRDISERGREIDGVIKQYNKFVKPAFDQYIQPTMRVADIVVPRGSGNTVAIDLIVQHVHSQLEEGSAGFCSPVPPAPQDPQCPQEHTAGQGDAYHHPSSTVQTPQGEDYEGCTYSGKQVSPTSERSLEGKPGDYLLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0I3</accession>
    <name>A0A6I9Z0I3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Polycystin-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="36" type="primary">PKD2</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the polycystin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930503.1">
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="POLYCYSTIN-2"/>
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    <keyword evidence="9" id="KW-0633">Potassium transport</keyword>
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            <end position="946"/>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="1134" mass="129060" checksum="853C676DFF89E9AF" modified="2020-10-07" version="1">MAGAGGGGGRAGLCPPGGLEQLELGQLGPPSGSPSPPLSSCSRQAWSRDNPVFEPEEEDPPRVATAVAGPVLRMDVEWGRGEAASPTPDAAQGRQRRRRRRGDEEEEEEEEGGGERGRPFPAAAGDALPARFAPEEGDGEAAEPPPHRIAWPKGLASRLRGLWGTRLMEETTTRERYLKSVLRELITYLIFLVVLSILSYGMVNSNIYYYTRVMSQLFLDTPVSKMEKTNFRSLSTMGDFWKFTEGPLLDGLYWEMWYNNKTVAENRSFIYHENLLLGVPRIRQLKVRNASCSIPEDLKDEIKDCYDVYSVTNEDTAPFGLRNGTAWTYTGEKDLNGSSHWGLIATYSGSGYYQDLSKSREETSALIAVLKNNLWLDRGTRAVFIDFSVYNANINVFCIVRLLVEFPPTGGLLTSWQFQPVKLIHYISTFDFFLAACEIVFCLFVIYYVVEEILEIHIHRLFYFRNLWNCLDVLIITLSLAAIGLNIYQISTVDKLLKKLLEGQNSFPNFEPLAYWQTQFNNVAAVTMFFVWIKLFKFISFNRTMNQLSTTMSRCAKDVLGFAIMFFIIFLAYAQLAYLVFGTQVNDFSTLQDSVFTQFRILLGDFDFTEVEEANRILGPVYFTSFVFFMLFILLVGNFFLFLDIFVAGPLFKWKFILRYYFSIQLIRVLCYVSMYQKNKKKKGLNLTRSMYLPNPYTGNGRISLLYYYTKKWADLANIKSKRCRFYASLSGLGQLAMANSLWPFWHGTICHGTRVTRITKEVVEFTEGPLLDGLYWEMWYNNKTVAENRSFIYHENLLLGVPRIRQLKVRNASCSIPEDLKDEIKDCYDVYSVTNEDTAPFGLRNGTAAQSFPVYPHKEYIALLFQGYTKAMVKLKLKKTPVDDISESLRHCGGKLNFDELRQNLKGKGRTDAEIEAIFTKYDQDGDQELTELEHQQMRDDLEKEREDLELDRNSLNRPLSGRSFPRSLDDSEEDDDDDSGHSSRRRGSSSSGVSYEEFQVLVRRVDRMEHSIGSIVSKIDAVIVKLETMERVKLKRKEVLGRLLDGVAEDERLGCENEAHRDQMERLVREELEHWESDDVASQVSQRLGTPVGFNSHGRTRNPRPSSSQSTEGIDGGGGKEGGGGGNRGSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1I7</accession>
    <name>A0A6I9Z1I7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box protein I2-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106556414</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930888.1">
        <property type="nucleotide sequence ID" value="XM_014075413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1I7"/>
    <dbReference type="GeneID" id="106556414"/>
    <dbReference type="KEGG" id="tsr:106556414"/>
    <dbReference type="OrthoDB" id="5402974at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF384">
        <property type="entry name" value="FORK-HEAD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="7">
        <location>
            <begin position="115"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="115"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013930888.1"/>
        </source>
    </evidence>
    <sequence length="270" mass="29637" checksum="A007B56688FBBF71" modified="2020-10-07" version="1">MDGLEVQPPLSARDLLDAAAPFCDPRSLLPTSQPPKVGYGRGESSGGGGGPYLWLNGAPSPYLPGANGAPGSFVPSAAYGTGARQLLAAQAAVGPPELSWLSLASHQQDFFKLVRPPYSYSALIAMAIQSAPSNRLTLSQIYQYVAATFPFYKKSKGGWQNSIRHNLSLNDCFKKVARLQDDPGKGNYWMLDPNCEKMFDNGNFRRKRKRRSDAASGPGVHNKMGDKTAFLEIKCNYQLVRVSARNNDRNISEIVYIKDVDIKFKKVDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2B3</accession>
    <name>A0A6I9Z2B3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein Jade-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Jade family PHD finger protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">PHD finger protein 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">JADE1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the JADE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930369.1">
        <property type="nucleotide sequence ID" value="XM_014074894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2B3"/>
    <dbReference type="GeneID" id="106555950"/>
    <dbReference type="KEGG" id="tsr:106555950"/>
    <dbReference type="CTD" id="79960"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15671">
        <property type="entry name" value="ePHD_JADE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15679">
        <property type="entry name" value="PHD_JADE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000004">
        <property type="entry name" value="Jade family PHD finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000030">
        <property type="entry name" value="Protein Jade-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039546">
        <property type="entry name" value="Jade-1_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF79">
        <property type="entry name" value="PROTEIN JADE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="23">
        <location>
            <begin position="227"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="24">
        <location>
            <begin position="279"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="615"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="672"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="755"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="616"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="805"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00041998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013930369.1"/>
        </source>
    </evidence>
    <sequence length="862" mass="98185" checksum="BC448FC31CE34DC1" modified="2020-10-07" version="1">MKRGRLPSSSEDSDDNGSLSTWSQHSRSQPQRSSCSKNKDRKPSELAPSNLSSGQEIRELNCCHSPCQHQVFRTDLITAMKLPDSSQLNPDEYYVLADPWRQEWEKGVQVPVSPGTVPEPVARIVSETKAVVFSRPRKYILSSGSEPPELGYVDIRTLAESVCRYDLNYMDVAWLELANEEFKEMGMPELDEYTMERIMEEFEQRCYDNMNHAIETEEGLGIEYDEDVVCDVCQSPDGEDGNEMVFCDKCNICVHQACYGILKVPEGSWLCRTCALGVQPKCLLCPKKGGAMKPTRSGTKWVHVSCALWIPEVSIGSPEKMEPITKVSHIPSSRWALVCSLCNEKIGASIQCSVKNCRTAFHVTCAFDRGLEMKTILADNDEVKFKSYCLKHSSTKKLDEEILNELTGQENENGAHDHALGHLESYDSIAQNQEEAHRVSIRKQKLQQLEDGFYAFVNASEVAKALQLPEDSVEFLYQYWKLKRKSNFNKPLITPKKDEEDNLAKREQDVLFRRLQLFTHLRQDLERVRNLTYMVTRREKIKRSVCKVQEQIFNLYTNLVEHERASGMPSSFSSMENLVLFNSLPLNPDAPKIEDLKWHSAFFRKQMGMSLNHSLKKTEKREQMRRNSGHNNKPLLKQPSQKEALDSSVSFFSFEKTFAEAQFGAAQQKNGIVTEDHRKRDNHSHCDTIKVGLKEKPPKHNYKPLKSNESFQRQLENKRAVNHPSGRSAPGIRRDMVPKCNGSFFRINCNPTPVKVPTTPRSPVKNWGGFRIPKKGERQQQGEGQEEACRQNSNFSYSALGQVSPKDRAKNKLKVDSENDGYAPDAEMSDSETEMAEKKCRLGSNSTLPRRTDIIRRSILAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2D3</accession>
    <name>A0A6I9Z2D3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Attractin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106555663</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930030.1">
        <property type="nucleotide sequence ID" value="XM_014074555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2D3"/>
    <dbReference type="GeneID" id="106555663"/>
    <dbReference type="KEGG" id="tsr:106555663"/>
    <dbReference type="OrthoDB" id="9998912at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056732">
        <property type="entry name" value="GBD_ATRN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF4">
        <property type="entry name" value="ATTRACTIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24972">
        <property type="entry name" value="GBD_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Attractin GBD" evidence="3">
        <location>
            <begin position="48"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930030.1"/>
        </source>
    </evidence>
    <sequence length="125" mass="13955" checksum="564EFC0929CFC767" modified="2020-10-07" version="1">CTCSGHANICHMQTGKCFCTTKGIKGDQCQLCDSENRYLGNPLRGTCYYSLLIDYQFTFSLLQEDDRHHTAINFIANPEQSNKNLDISINASNNFNLNITWSIGSTGHPESLTEIATQIAVEMNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2I5</accession>
    <name>A0A6I9Z2I5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Delta-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">JAG1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Putative Notch ligand involved in the mediation of Notch signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 17">Membrane</location>
            <topology evidence="2 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930842.1">
        <property type="nucleotide sequence ID" value="XM_014075367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2I5"/>
    <dbReference type="GeneID" id="106556372"/>
    <dbReference type="KEGG" id="tsr:106556372"/>
    <dbReference type="CTD" id="182"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055123">
        <property type="term" value="P:digestive system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061008">
        <property type="term" value="P:hepaticobiliary system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042491">
        <property type="term" value="P:inner ear auditory receptor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090596">
        <property type="term" value="P:sensory organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030878">
        <property type="term" value="P:thyroid gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035239">
        <property type="term" value="P:tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000007">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000117">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000148">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000181">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000229">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000431">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000613">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.140:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3510:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000146">
        <property type="entry name" value="Putative neurogenic locus notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056986">
        <property type="entry name" value="JAG1_1/2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026219">
        <property type="entry name" value="Jagged/Serrate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF12">
        <property type="entry name" value="DELTA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01414">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23575">
        <property type="entry name" value="JAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02059">
        <property type="entry name" value="JAGGEDFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 17" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 15 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 15 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 17 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026961979" description="Delta-like protein" evidence="20">
        <location>
            <begin position="34"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1070"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="domain" description="DSL" evidence="22">
        <location>
            <begin position="185"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="230"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="296"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="336"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="374"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="412"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="450"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="487"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="525"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="591"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="630"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="668"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="706"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="745"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="783"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="821"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1174"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1191"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="656"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="694"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="732"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="771"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="809"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="847"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU280815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013930842.1"/>
        </source>
    </evidence>
    <sequence length="1220" mass="134380" checksum="AD808B6EC02BCF8F" modified="2020-10-07" version="1">MLLSKRRSQLTEFSLSLFLALLCLRVKVTLTSGQFEFEIISMQNVNGELQNGNCCDGTRNPIDKKCTRDECDTYFKVCLKEYQSRVSAEGACSFGLGSTPVIGGNTFNLRYSRNNERNRIVLPFSFAWPRSYTLLVEAWDYSNDSASADSIIEKASHSGMINPSRQWQVLKQNSGVAHFEYQIRVTCDEHYYGFGCNKLCRPRDDFFGHYTCDQNGNKTCLEGWMGPDCNKAICRQGCNPRHGSCKIPGECRCQYGWQGQYCDKCIPHPGCVHGTCNEPWQCLCETNWGGQFCNKDLNYCGTHPPCLNGGTCSNTGPDKYQCSCPEGYSGQNCEIAEHACLSDPCHNGGSCLETPTGFECVCALGWAAPTCMLNIDDCSPNPCGHGGTCQDLVDGFKCVCPPQWTGKTCQIDANECEGKPCINANSCRNLIGSYYCNCIAGWTGQNCETNIDDCVGQCQNGGTCKDLVNGYRCLCPPGYIGDHCERDINECASNPCLNGGHCQDEINGFQCLCLAGFSGNICQLDVDYCEPNPCQNGAQCFNYESDYFCSNCPDDYEGKNCSHLKDHCRSTPCEVIDSCTVAVASNSTPEGVRYISSNVCGPHGKCKSQSGGKFTCECNKGFTGTYCHENINDCQSNPCKNGGTCIDGINSYKCICSDGWEGTFCEANINDCSKSPCHNGGTCRDLVNDFFCECKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGNVFKCMCPAGWDGATCNIARNSSCLPSPCHNGGTCVVSGESFTCVCKEGWEGPTCTQNTNDCSPHPCYNNGNCVDGENWYRCECAPGFAGPDCRININECQSSPCAFGATCIDEINGYRCICPPGRGGPICQEVSGRPCIASGHIIPDGSKWDDECNSCQCVNGKVSCSKVWCGPKPCVINAKGHSECPAGHTCHPVKADHCFVPPCDNAGECWPSNQQPVKMTCSSDSYYQEHCANITFTFNKEMMTPGLTTEHICSELRNLNILRNVSIEYALYITCEPSHLASNEIHVAISAEDIQEENNPVKEITDKIIDLVSKRDGNSTLIAAVAEVRVQRRPIKSKTDFLVPLLSSILTVAWICCLVTAFYWCIRKRRKQSTHTHTASEDNTTNNVREQLNQIKNPIEKHGANAVPIKDYENKNSKIAKIRTHNSEVEEDDMDKHQQKTRFAKQATYTLVDRDEKPPNGTPTKHPNWTNKQDNRDLENAQSLNRMEYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2M2</accession>
    <name>A0A6I9Z2M2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formin-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930479.1">
        <property type="nucleotide sequence ID" value="XM_014075004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2M2"/>
    <dbReference type="GeneID" id="106556044"/>
    <dbReference type="KEGG" id="tsr:106556044"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000036">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000045">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF5">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="2">
        <location>
            <begin position="23"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="381"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930479.1"/>
        </source>
    </evidence>
    <sequence length="502" mass="58050" checksum="44D3B075A231E895" modified="2020-10-07" version="1">MGNAGSMDSQQTDFRAHNLPLKLPMPEPGELEERFAVVLNAMNLPPDKARLLRQYDNEKKWELICDQERFQVKNPPHTYIQKLKGYLDPAVTRKKFRRRVQESTQVLRELEISLRTNHIGWVREFLNEDNKGLDVLVEYLSFAQYAVTFDFESLENNMENSVDKNKPWSRSIEDLHRGSNLPSPIGNSISCSSRHSTLRYNTLPSRRTLKNSRLVSKKDDVHVCIMCLRAIMNYQYGFNMVMSHPHAVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHEIILSAFDNFKEVCGEKQRFEKLMEHFRNEDNNIDFMVACMQFINIVVHSVEDMNFRVHLQYEFTKLGLDEYLDKLKHTESDKLQVQIQAYLDNVFDVGALLEDAETKNAALERVEELEENISHLSEKLQDTENEAMAKIVELEKQLMQRNKELDVVREIYKDANSQVHTLRKMVKEKEEAIQRQSTLEKKIHELEKQGSIKIQKKGDGDINILPVGVSPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z390</accession>
    <name>A0A6I9Z390_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106556994</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013931558.1">
        <property type="nucleotide sequence ID" value="XM_014076083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z390"/>
    <dbReference type="GeneID" id="106556994"/>
    <dbReference type="KEGG" id="tsr:106556994"/>
    <dbReference type="OrthoDB" id="5232919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000002">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF41">
        <property type="entry name" value="HISTONE DEACETYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="12">
        <location>
            <begin position="58"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931558.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="38554" checksum="6F3319D575DEBEE8" modified="2020-10-07" version="1">MVHVDDNQPSARTQISLTTCVLPHPTVDQTIQQTSMTGVAYDTLMLKHQCLCGNYTSHPEHAGRIQSIWSRLQECGLLNKCERIRGRKASLEEIQLVHSEHHSLLYGTSPLNRQKLDPRKILDSALHKPFSLLPCGGLGVDSDTIWNELHSSGAARMAVGCVIELASKVVSGELKNGFAIVRPPGHHAEESTAMGFCFFNSVAITAKYLRDKLNIGKILIVDLDVHHGNGTQQAFYSDPSILYVSLHRYDEGNFFPGSGAPNEVGSGPGEGYNINIAWTGGLDPPMGDVEYLTAFRTVIMPITSEFNPDFVLVSAGFDVVEGHESPLGGYKVTAQCMYYFFLKKICVLTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3M8</accession>
    <name>A0A6I9Z3M8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger and BTB domain-containing protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ZBTB16</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930834.1">
        <property type="nucleotide sequence ID" value="XM_014075359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3M8"/>
    <dbReference type="GeneID" id="106556365"/>
    <dbReference type="KEGG" id="tsr:106556365"/>
    <dbReference type="CTD" id="7704"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18205">
        <property type="entry name" value="BTB_POZ_ZBTB16_PLZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001013">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000059">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF6">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="34"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930834.1"/>
        </source>
    </evidence>
    <sequence length="477" mass="51977" checksum="D599DE548BF06D33" modified="2020-10-07" version="1">MDLTKMGLIQLQNPSHATGLLQKANQMRLAGTLCDVVILVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKVEDLDDLLYAAEILEIEYLEEQCLKILETIQASEDNEAEVSMTDGGAEDEEDRKSRYIKNILLSKHSSEESGYSSLAGQGLMGTMVEHSPSVSTSFGLAAMSPTKTAVDSLMSIGQSLLQGALPRQALEDLHSANGRCPAVSELKTEAMQVEEASSHESPRSGELGASGGDKAEDKSKQGPGTPTRSSVITSARELHYPRDDNAEQPLEAVQGPPVGPDLLASHGEKPMGMYSVLPNHKGESMLGLPVSMAPTLHVQPALAVSMDFGSYGGLLPQGFIQRELFNKLGELAVGIKTEHRSVGEQCSVCGVELPDNETVEQHRKLHSAMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFLCDHCGAQFSKEDALESHRQTHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3Y1</accession>
    <name>A0A6I9Z3Y1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rap guanine nucleotide exchange factor 3-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106556124</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930570.1">
        <property type="nucleotide sequence ID" value="XM_014075095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3Y1"/>
    <dbReference type="GeneID" id="106556124"/>
    <dbReference type="KEGG" id="tsr:106556124"/>
    <dbReference type="OrthoDB" id="21144at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000015">
        <property type="entry name" value="Rap guanine nucleotide exchange factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="83"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="198"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="4">
        <location>
            <begin position="338"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930570.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="58777" checksum="D28AD2404FEBE7F6" modified="2020-10-07" version="1">MHLFRSYSYRIQLEGQLNNAPRIQGIRWTPLPESESTLVPSHNLKQASSNKIRRAGRLLCAHLVVMYPDLIRDHKHHLRLHRQCCSGKELVDALLSAGLSVQTRSQALGLCQVLMDEGVLTHVRQESYFQDRDAQFFRFVALEPSAEDRDGEDLLEALALLTQLGPTALLTTILRKPPNQRTDEELALIFEELLHVKAVAHLSSSVRRELAAVLLFESHAKAGTVLFSQGDKGTSWYIIWKGSVNVVTHDKGLVCTLHEGDDFGQLALVNDAPRAATIILREDSCHFLRVDKRDFNRILKDVEANTLRLKEHGKVVLVLEKNSQGSNSSHLAGTLGSSRYSVIAGTPEKILDYLLETMRSDSTLSDPVDTFLGDFLLTYSVFMPTPQLCRTLLQHFHSEPSEGSEQDKAAYILRKRQKILWLVNRWVSLYGRLLQAETCAVAFLQTMSECATQDARLGPLLREQIQDRRRNRVIENGNNCASPKLQVRNAVNWFAVQEEPFLNSSYTIRMKDKGHHDFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z411</accession>
    <name>A0A6I9Z411_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor AP-2-epsilon-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930964.1">
        <property type="nucleotide sequence ID" value="XM_014075489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z411"/>
    <dbReference type="GeneID" id="106556495"/>
    <dbReference type="KEGG" id="tsr:106556495"/>
    <dbReference type="OrthoDB" id="6252992at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF13">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2-EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930964.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="17705" checksum="FCB9D3164AD96B0F" modified="2020-10-07" version="1">MLVHTYSAMDRSDGLNGTSAGGRLPQLSSLNQAAAYSSAPPLCHTPASDFQPPYFPPPYPQPPLPYAQSQESAYPHLGDPYGGLNPIHQHQQSGWPSQRARQEEAAAAAAAAAAGLLSQTHRALGLDPRRDYPGMPRLLHGLADGAHGLADGPLGLHALAHPSLEDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5Z7</accession>
    <name>A0A6I9Z5Z7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">OXSM</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">May play a role in the biosynthesis of lipoic acid as well as longer chain fatty acids required for optimal mitochondrial function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:41853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41900"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:41901"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17 19">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931361.1">
        <property type="nucleotide sequence ID" value="XM_014075886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5Z7"/>
    <dbReference type="GeneID" id="106556865"/>
    <dbReference type="KEGG" id="tsr:106556865"/>
    <dbReference type="CTD" id="54995"/>
    <dbReference type="OrthoDB" id="5334845at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000015">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000024">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="20">
        <location>
            <begin position="42"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="18">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013931361.1"/>
        </source>
    </evidence>
    <sequence length="462" mass="48863" checksum="AF1FDBA0FA8FEE98" modified="2020-10-07" version="1">MMILQHLRIVFRATSLCLPSSQLQQYLNINKRCLNTRLVHGQRRVVVTGIGLVSPLGVGTQLVWNNLLQGNCGVVALEGEEYASIPCKVAACVPKGVDEGHFHEENFVIKSESKTMSSATIMAIGAADLALKDSGWSPQSDQDSLKAGVAIGMGMVPLENISDTALTFKTKGYNKVSPFFVPKILINMAAGHISIKHQLKGPNHAVSTACTTGAHAIGDSFRFITHGDADMMLAGGTEACISPLSLAGFARARALSTSFNSNPQKACRPFHPLREGFVMGEGAAVLVLEEYMHAIKRGVKPYAEILGYGLSADACHITAPDPNGDGALRCMSAAIKDSGISLEDVTYINAHATSTPLGDAAENQAIKDLFKDHASALAVSSTKGATGHLLGAAGAIEAAFTALACYHGILPPTLNLDQTEPLFDLNYVPLVPQEWKSTKRLIALSNSFGFGGTNASLCFASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZN7</accession>
    <name>A0A6I9WZN7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ethylmalonyl-CoA decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ECHDC1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907586.1">
        <property type="nucleotide sequence ID" value="XM_014052111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZN7"/>
    <dbReference type="GeneID" id="106537842"/>
    <dbReference type="KEGG" id="tsr:106537842"/>
    <dbReference type="CTD" id="55862"/>
    <dbReference type="OrthoDB" id="448450at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF27">
        <property type="entry name" value="ETHYLMALONYL-COA DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026841471" evidence="2">
        <location>
            <begin position="23"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907586.1"/>
        </source>
    </evidence>
    <sequence length="177" mass="19343" checksum="170176B363AEBA57" modified="2020-10-07" version="1">MNMCMFMQNILTRLMRLPLITAALVQGKALGGGAELTTACDFRLMTPESEIRFVHKQMGLVPGWGGAARLTQILGSSSALKLLSGAVKLDPQNALHIGLVDSILSSSDEHESLREMHTWLKLYTTGPAEVIQAVKKVVSAGRELQLEAALRREKEIFGRVWGGPANLQALAQRTKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0U9</accession>
    <name>A0A6I9X0U9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106537734</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907417.1">
        <property type="nucleotide sequence ID" value="XM_014051942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0U9"/>
    <dbReference type="GeneID" id="106537734"/>
    <dbReference type="KEGG" id="tsr:106537734"/>
    <dbReference type="OrthoDB" id="8612291at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013907417.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="24673" checksum="CC3EEB2BF6264370" modified="2020-10-07" version="1">MALVNRTIMQLAGILFCLLGWVLSCLTTYLPQWKNLNLELNELEIWTMGLWQACVVHEEGGMQCKEFDSFLALPPELQISRILMFISNGLGLSGLLLSSFGLDCLKIGEQQQDLKKRLLLLGGIFCWISGAAALVPVSWVAHTTVQEFWDETIPDIVPRWDFGEALFIGWFAGFCLIFGGSLLNCSVCSVDTQSSSVHYTVAGVLDACQHLESNNRPQSLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X166</accession>
    <name>A0A6I9X166_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sodium- and chloride-dependent glycine transporter 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106537823</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907557.1">
        <property type="nucleotide sequence ID" value="XM_014052082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X166"/>
    <dbReference type="GeneID" id="106537823"/>
    <dbReference type="KEGG" id="tsr:106537823"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF241">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GLYCINE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013907557.1"/>
        </source>
    </evidence>
    <sequence length="389" mass="44243" checksum="8A5F7CD8DBA0FB52" modified="2020-10-07" version="1">MRTDDVGKGYPRVVYFTATFPYVVLIILLIRGVTLPGAGAGMWYFITPKWEKLIDAMVWKDAATQIFFSLSAAWGGLITLSSYNKFHNNLYRDTLIVTCTNSATSIFAGFVIFSVIGFMANELKVDIEDVADQGPGIAFVVYPEALTRLPLSPFWAIIFFLMLLTLGLDTMFATIETIVTSVSDEFPKYLRTHKPLFTLGCCISFFIMGFPMITQGGMYMLQLVDTYAASYSLVIIAIFELVGISYIYGLERFCEDIEMMIGFQPNRFWRVCWAFVTPTILTFILSFSFYQWEPMTYATYHYPGWSMVLGWLMLACSVIWIPIMFVIKMHLAPGNFIERLKLVCSPQPDWGPFLAKHRGVRYQNMIDPLGTSSLGLKLPPKDFELEMQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1H4</accession>
    <name>A0A6I9X1H4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytosolic non-specific dipeptidase</fullName>
            <ecNumber evidence="13">3.4.13.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">CNDP dipeptidase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Threonyl dipeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">CNDP2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. Mediates threonyl dipeptide catabolism in a tissue-specific way. Has high dipeptidase activity toward cysteinylglycine, an intermediate metabolite in glutathione metabolism. Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis. Plays a role in the regulation of cell cycle arrest and apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(S)-lactate + L-phenylalanine = N-[(S)-lactoyl]-L-phenylalanine + H2O</text>
            <dbReference type="Rhea" id="RHEA:66724"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:167456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:66725"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:66726"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolysis of dipeptides, preferentially hydrophobic dipeptides including prolyl amino acids.</text>
            <dbReference type="EC" id="3.4.13.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-cysteinylglycine + H2O = L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:28783"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:28784"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-L-threonine + H2O = L-threonine + L-serine</text>
            <dbReference type="Rhea" id="RHEA:67372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:169955"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:67373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-L-serine + H2O = L-threonine + L-serine</text>
            <dbReference type="Rhea" id="RHEA:67364"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:169954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:67365"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-L-threonine + H2O = 2 L-threonine</text>
            <dbReference type="Rhea" id="RHEA:67360"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:169953"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:67361"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="25">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="25">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.18" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013908186.1">
        <property type="nucleotide sequence ID" value="XM_014052711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1H4"/>
    <dbReference type="GeneID" id="106538260"/>
    <dbReference type="KEGG" id="tsr:106538260"/>
    <dbReference type="CTD" id="55748"/>
    <dbReference type="OrthoDB" id="7832001at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05676">
        <property type="entry name" value="M20_dipept_like_CNDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000008">
        <property type="entry name" value="Cytosolic non-specific dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000014">
        <property type="entry name" value="Cytosolic non-specific dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017153">
        <property type="entry name" value="CNDP/DUG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF11">
        <property type="entry name" value="CYTOSOLIC NON-SPECIFIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037242">
        <property type="entry name" value="CNDP_dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 25" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="27">
        <location>
            <begin position="209"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="active site" evidence="23">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="23">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="24">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="24">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="24">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="24">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="26">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013908186.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="53245" checksum="E551D1D5BAAC03D9" modified="2020-10-07" version="1">MASALESLFEYVDKHQDLYVERLAQWVAIQSVSAWPEKREEIRRMMEVAAKDIERLGGTTELIDVGKQTLPDGSKIPLPPIILGKLGSDPCKKTVCVYGHLDVQPAALEDGWDSEPFVLIEREGKLYGRGSTDDKGPVLAWLNALEAYQKTKQEIPVNVKFCLEGMEESGSEGLDDLIFAQKDTFFKDVDYVCISDNYWLGKKKPCITYGLRGICYYFIEVECSDKDLHSGVFGGSVHEAMTDLIALMGSLVDKKGKILIPGIYETVASLTNEEKQLYDKIDFDLKEYAKDVGATKLLHDTKEMILMHRWRYPSLSLHGIEGAFSGSGCKTVIPRKVIGKFSIRLVPDMTPEVVTKRVEEYLSKKFAELHSPNKFSVKLGHGGKPWVSDFNHPHYMAGRRAMKTVFGVEPDLTREGGSIPVTLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRYNYIQGVKLLGAYLYEVAKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1Y3</accession>
    <name>A0A6I9X1Y3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein shisa-2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SHISA2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909264.1">
        <property type="nucleotide sequence ID" value="XM_014053789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1Y3"/>
    <dbReference type="GeneID" id="106539104"/>
    <dbReference type="KEGG" id="tsr:106539104"/>
    <dbReference type="CTD" id="387914"/>
    <dbReference type="OrthoDB" id="10025410at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040037">
        <property type="term" value="P:negative regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395:SF0">
        <property type="entry name" value="PROTEIN SHISA-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395">
        <property type="entry name" value="SHISA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027005237" evidence="7">
        <location>
            <begin position="36"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="8">
        <location>
            <begin position="37"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013909264.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="32057" checksum="8AE225670E3D63BA" modified="2020-10-07" version="1">MWLGRRWQLLAAPGGRLLLLLLCLLVGLRARGALGSGEYCHGWQDGASWREGFQCPEHFDEGDATICCGTCALRYCCSRPEVRLDQGMCDNDRQHGGAEHGRPDKDMPDSAAVPIYVPFLIVGSVFVAFIILGSLVAACCCRCLRPKQEPQQSRAPGGNRIMETIPMIPSASTSRGSSSRQSSTAASSSSSANSGARAAPTRSQANCCLPEGTMNNVYVNMPTNFSVLNCQQATQIVPHQGQYLHPQYVGYAVQHDSMPMASVPPFLDSLQSGYRPVQSPYPHSNSEQKMYPAVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2T1</accession>
    <name>A0A6I9X2T1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 151B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM151B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM151 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909584.1">
        <property type="nucleotide sequence ID" value="XM_014054109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2T1"/>
    <dbReference type="GeneID" id="106539332"/>
    <dbReference type="KEGG" id="tsr:106539332"/>
    <dbReference type="CTD" id="441151"/>
    <dbReference type="OrthoDB" id="190434at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026767">
        <property type="entry name" value="Tmem151"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31893">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 151 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31893:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 151B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14857">
        <property type="entry name" value="TMEM151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909584.1"/>
        </source>
    </evidence>
    <sequence length="486" mass="56774" checksum="D9566BE8E15D8FE3" modified="2020-10-07" version="1">MSPHHAKQQRPVKQSLSKSLCRESHWKCLVLSFLMYGCMGAMTWCHVTKVTRLTFDSAYKGKSMMYHDSPCSNGYIYIPLAFLVMLYVVYLVECWHCYTRNELQYKVDVDSVHERVQRMQQATPCIWWKAISYHYVRRTRQVTRYRNGDAYTTTQVYHERVNTHVAEAEFDYSNCGAKDISKDLIDLESFPATRLRFTKCFSFANVESENSYLTQRARFFTENEGLDDYMEAREGMHLKNVDFKEYMVAFSDPENLPWYISHYVFWVAALLTLSWPLRVLNEYRTSYVHYHIEKLFGFDSVAVTPAEERPFCRRMPRVNTVDSTELEWHIRSNQQLVPSYSEAVLMDLVGLSGCTTYSSCHYGSYRQNCEQCHRTISSSSIFSRSALSICNGSPRIPFSSSRFSLGRLYGSRRSCLWQSRSESLNEQSCPTEQTRLSSQVTMEEEEPPPYQDALYFPVLIVHRNEGCLNHDHRHLHRNGSCVETSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2W3</accession>
    <name>A0A6I9X2W3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DnaJ homolog subfamily C member 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">DNAJC7</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes. Recruits NR1I3 to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908177.1">
        <property type="nucleotide sequence ID" value="XM_014052702.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908178.1">
        <property type="nucleotide sequence ID" value="XM_014052703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2W3"/>
    <dbReference type="GeneID" id="106538256"/>
    <dbReference type="KEGG" id="tsr:106538256"/>
    <dbReference type="CTD" id="7266"/>
    <dbReference type="OrthoDB" id="765884at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000018">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000097">
        <property type="entry name" value="DnaJ homolog subfamily C member 7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45188:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45188">
        <property type="entry name" value="DNAJ PROTEIN P58IPK HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="223"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="269"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="341"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="15">
        <location>
            <begin position="394"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013908177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013908178.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="57357" checksum="E8F91151ACE50931" modified="2020-10-07" version="1">MATAGGDGDVIMAPAAPPTVASHSSMPLMLSEEDEGTRREAESFKEQGNAFYVRKDYNEAYNYYTKAIDACPSNASYYGNRAATLMMLGRFREALGDAQQSVRMDDSFLRGHLREGKCHLSLGNAMAASRCFQRVIELDHENPQAEQELKSAKAVLDYEKIAEVDFEKRDFRKVVFCMDRALEFAPACHRFKILKAECLALLGRYSEAQSVISDILRIDSTNADALYVRGLCLYYEDCIEKAVQFFVQALKMAPDHDKACLACRNAKALKAKKDEGNKAFKEGNYKLAFTLYTEALAIDPNNRKTNAKLYCNRGTVNSKLGKLCEAIGDCTSAIKLDDTYIKAYLRRAQCYMDTEQYEDAVRDYEKVYQTEKTKEHKQLLKNSQVELKKSKRKDYYKILGVDKNASEDEIKKAYRKRALMHHPDRHSGASAEIQKEEEKKFKEVGEAFTILSDPKKKARYDSGQDLEEDGMNMGDFDANNIFKAFFGTPGSFSFEASGPGNFFFQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4F6</accession>
    <name>A0A6I9X4F6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein C9orf72 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106539848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910229.1">
        <property type="nucleotide sequence ID" value="XM_014054754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4F6"/>
    <dbReference type="GeneID" id="106539848"/>
    <dbReference type="KEGG" id="tsr:106539848"/>
    <dbReference type="CTD" id="203228"/>
    <dbReference type="OrthoDB" id="10252077at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027819">
        <property type="entry name" value="C9orf72"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31855">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE C9ORF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31855:SF2">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR C9ORF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15019">
        <property type="entry name" value="C9orf72-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51835">
        <property type="entry name" value="DENN_C9ORF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013910229.1"/>
        </source>
    </evidence>
    <sequence length="480" mass="54360" checksum="FCC4AAF263ED9EDC" modified="2020-10-07" version="1">MSALCPPPSPAVAKTEIALSGESPLLAATFAYWDNILGPRVRHIWAPKTEQQLLSDGEITFLANHTLNGEILRNAESGAIDVKFFVLSEKGVIIVSLIFDGNWNGDRSTYGLSIILPQSELSFYLPLHRVCVDRLTHIIRKGRIWMHKERQHFQKSIMEGAERVEDQGQSIIPMLTGEVIPVMELLSSMKSHSVPEEIDIRDTVLSDDDIGDSCHESFLLKAISSHLQTCGCSVVIGSSAEKVNKIVQTLCLFLTPAERKCSRLCRNETSFKYESGLFVQGLLKDVTGSFVLPFRQVMYAPYPTTHIDVDVNTVKQMPPCHEHIYNQRRYMRSELTAFWRATLDEEMPLDTIIYTDESFTPDLNVFQDVQHQDTLVKAFLDQVFHLKPGLSLRSVFLAQFLLVLHRKALTLIKYIEDDTQKGKKPFKSLRNLKIDLDLTAEGDLNIIMAFAEKLKPGLHSFLFGQPFYTSVQEHDVLMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7A2</accession>
    <name>A0A6I9X7A2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamine and serine-rich protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">QSER1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910136.1">
        <property type="nucleotide sequence ID" value="XM_014054661.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539775"/>
    <dbReference type="KEGG" id="tsr:106539775"/>
    <dbReference type="CTD" id="79832"/>
    <dbReference type="OrthoDB" id="8447576at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052466">
        <property type="entry name" value="DNA_MethProtect_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025451">
        <property type="entry name" value="DUF4211"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14709:SF2">
        <property type="entry name" value="GLUTAMINE AND SERINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14709">
        <property type="entry name" value="GLUTAMINE AND SERINE-RICH PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13926">
        <property type="entry name" value="DUF4211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4211" evidence="2">
        <location>
            <begin position="1536"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1478"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1493"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1511"/>
            <end position="1527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013910136.1"/>
        </source>
    </evidence>
    <sequence length="1624" mass="179484" checksum="65CAE1C3A8C930F8" modified="2020-10-07" version="1">MMDRNYPPAPSYPDPLGSAASASSQPATVWAYERGVSSLKPSLNYGGGHSSHSEADLLRQTYAASQQLPVYTTTPHPSGLPGIFDNSTHSSNSNTKEASVMNFLSAIESRNTQTVSSGTTLLPQFRAPTWQPAGMHPSSELFVTGALPTSGTFPSTSVYQHPNAFSSRNFVTTPSLTLQDTTFSPTSNGLLTAHDPLLQIKTPQGTVPPALTFDRLGSYVLSTSIPPQSSTYRSAQESAPHLLQPQFSLLPSSLGGGPQQTPQVYNSTLFTGSTASIERALQRECSVIKHHQRPSNTQSVQAQLTSSQHSLQNYLPSTDESDFQDAPRPSTCSPAGDATQVSSSGPQQKTSQVSVELSQSYASAVPSPGFQSVSTTKAKQCSTKQPTRSTKTPKPQSAAPSVQTQSYSKIAHNQNSVISGQAQIYSTAQLPSLLSVSQSQNYISTQSQNVPPVSHAQVFSTIKTEKLPSLYKTLSFAGQSEVTTSESQALPYTSDQQVLTSVSNTFSGQTRDLSSSSQSENYSSSHSQGMSTISQPQISYSSQSQVLSVVSPSETFSSHIQNLSTTSPPQNYISLHSQVQESSSSQSQNFLPVVQSTPFEVRPNSPALQNNRSSSDTKSYIKRKSDPNLYQTSKQDDQFPMHNFQALQQQASLDSSPQRLTDGEINAQDTTYKVSKADNRYSQSVIRSNSRLEDQIVGLTFQGSKKEEIMVNSVTQISHITNTGNHDIKKAGSLLSTAQVNADSKELNQQESLMHKVHEVKVSEQQIHAVSSPSQLHTQPIRQSHQLSLPTSQVLLESPCDLQMLQQSILQSGLGYTKSVPQVQHIMNSQQYLQVDGHMIQGNGRHSQEQLHSQHTEVLKVNLSEPSKPLQHHMTPKDHFGQPNHESKNQFVPLSSVCFPESVLLNDERNILSNVDDILAATAAACGVTPSEFAKSTANEEEIQSVENGDIPKSQFHTIDVRHLTPSFNSSPALPGKPLNNISLNGGHVTLNLTAVPADQTKDRNLDQQHIEISAQGVPTRITTVEIERRQEQVSNQVKQQSSTRSESEAKNNVHMDGPLNIKDIDLISSRTLNEEITVSKTDYSMASDNADVPLQQIPMQHPRNENGNNTEDKSQLFSEEDPQKQKDRGQNQIKNVNEVDTNQKLLKRSGQCKRQNSRGIDANSPVPENCHETYQHQEKMRQKIKEVEEKQPEVRTGFIASFLDFLKSGPRQQFSAPAIRVPNRVRRPVTPLIRVPTATSSFDSFAENSVKNNYEDPKIKSEALPYFSFDNDNVRNNKDYQKSIYSPLSSSDTKIKSEIDKTYSQAITNTVVKKEASQMTVTVTDPQVKTCAAQFKTESVLTEPLAKPQVLVAIEGYTEDDHGDSDGEGIYRERDEFVVKIEDIEPLMAAFKRGVEPPAIWKVQKALLQKFVPEVRDGQREFAATNSYLGYFGDAKLKYKRVYVKFIENANRKEYVRVCSKKPKNSPVQSARIAHCKPSMNITKVPDPPVPKTVTTKVTSVKPKAKQPKTKAEPPPKKRKTWKEESPSTLQPQSDEEDTEPPAPIVARFLNTRAMKETFRNYMELLVSLALDPDTMQALEKSNDELLLPHMRKIDGMLNDNRKRLLSKLHLERPFKVLIIIFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7J4</accession>
    <name>A0A6I9X7J4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106537682</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907348.1">
        <property type="nucleotide sequence ID" value="XM_014051873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7J4"/>
    <dbReference type="GeneID" id="106537682"/>
    <dbReference type="KEGG" id="tsr:106537682"/>
    <dbReference type="OrthoDB" id="6145535at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15232">
        <property type="entry name" value="7tmA_OR13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="51"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013907348.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="36808" checksum="D5A4CFB0F4B07771" modified="2020-10-07" version="1">MTLKVFGHRMFEIKNHSAITEFILLGLTDSPQLQMFLFCVFAFIYATALAANILLMFIICIVKKLHTPMYFLVFNLSLVNILSISVITPRLLHSLLTNRKAISIHGCITQIYFFIWTLGTELFILSFMAFDRYAAICHPLQYTIIMRKEVCVGIAIFVWITGMLNTSIQAVLMLQLSFCDSNIINHFFCDIPPILDLTCSDTTIIHVSAIISDVLFGIVTCGLTLTSYFFILRTIFRIRSTEGKKKAFSTCSSHLFVISFYFSSVIYTYIRPPSSSLDKDKFIALLYTVVTPVVNPLIYSLRNKDVKDALKMLTVNLKMFPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X804</accession>
    <name>A0A6I9X804_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106540003</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910441.1">
        <property type="nucleotide sequence ID" value="XM_014054966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X804"/>
    <dbReference type="GeneID" id="106540003"/>
    <dbReference type="KEGG" id="tsr:106540003"/>
    <dbReference type="OrthoDB" id="9040591at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="229"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013910441.1"/>
        </source>
    </evidence>
    <sequence length="499" mass="55945" checksum="C667E2EF57C40421" modified="2020-10-07" version="1">MRLYEPYQFLQNLTLGYSIYDNYFNTLHTSDALLDMLSTGETNVPNYSCGRKDKLLALIDGADNDISIQMSTLVGTYKISQFHPFLKNNELCNLSLLKLYLDQNGDVAADLNIISWMFLSKEDPIREQLGSFEQQKLIVNQDSLSRLKLLNKSLPQSKCVESCHPGFVKQAREGEPLCCYDCIPCPEGTISTQEDAEKCTKCPDDQYPTKNRVRCIPKRIIFLSYEEHLSIILLFFALLLFLTTGFVLIIFLKYLDTPIVKANNRDLSYTLLVSLLLCFSSSSFFIGKPRKVICLLRQIVFSIVFSVAVSSVLAKTITVMLAFLAAKPGNKVRRWLGKSLANSIIFSCSGVQIFICALWLGVSPPFPDSDLHSQPGEIILQCNEGSVTMFYIVLGYMGFLATISFIVAFLARNLPGAFNEAKLITFSMLVFCSVWISFLPTYLSTKGKSMVAVQVFSILTSGAGLLSCIFFPKCYIIIFRPDLNTKEHLMIKVGKSNCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8X5</accession>
    <name>A0A6I9X8X5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPL17</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912024.1">
        <property type="nucleotide sequence ID" value="XM_014056549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8X5"/>
    <dbReference type="SMR" id="A0A6I9X8X5"/>
    <dbReference type="GeneID" id="106541190"/>
    <dbReference type="KEGG" id="tsr:106541190"/>
    <dbReference type="CTD" id="6139"/>
    <dbReference type="OrthoDB" id="10254664at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000003">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_uL22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057265">
        <property type="entry name" value="Ribosomal_uL22_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003260">
        <property type="entry name" value="PRK04223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013912024.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="21397" checksum="33379CCF610D1CA0" modified="2020-10-07" version="1">MVRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLKKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEVAQKKKISQKKLKKQKLMARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X942</accession>
    <name>A0A6I9X942_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Histone deacetylase 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HDAC10</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910267.1">
        <property type="nucleotide sequence ID" value="XM_014054792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X942"/>
    <dbReference type="GeneID" id="106539877"/>
    <dbReference type="KEGG" id="tsr:106539877"/>
    <dbReference type="CTD" id="83933"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019213">
        <property type="term" value="F:deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000005">
        <property type="entry name" value="histone deacetylase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF43">
        <property type="entry name" value="POLYAMINE DEACETYLASE HDAC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="5">
        <location>
            <begin position="52"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013910267.1"/>
        </source>
    </evidence>
    <sequence length="748" mass="83738" checksum="FC6E6AC14767750B" modified="2020-10-07" version="1">MLLVSSSGMVLLTGRSCSLSEAYVMAAGTALVYDGEMINHKLLWEDPDCNLEVPERLSSCYGCLQHYNLIERCISVSAREGTESEIMLIHSKDYVEIVKSTEKMGKEELQKVSAEYDAVYFHPTTYRCAKLAVGATLQLVDAVMSGKTRNGLTLIRPPGHHSQRSAANGFCIFNNVAIAAKYAQRKYGLQRVLIVDWDVHHGQGIQYAFDDDPSVLYFSWHRFEHQNFWPCLSESNYDAVGQGKGKGFTINLPWNQIGMGNSDYLAAFLHVLLPIAFEFDPELVLISAGYDSGIGDPEGQMKATPECFSHLTHFLMNLANGKLCAVLEGGYHLRSLSESVCMTLKALLGDPLPPLSGEMMPCLSARESIQNVRAAHKPYWKCLTYEDVTPVQDLSTTSCQAEKAELTQSNVELLSPQEVGKGDVNEVIKTDRFLETHMKSILSLKPPIKTAVAVDESGTQLLPPTVHIPKGLDMEEFKTTISAFHVKNMKEAALKSHLNMLAVLNKIIKKEVINGLAVSSTPSLSSAIAVAHCRNWKVEKILCVFVGDMDRKIELDDDKVLLINICAKHSEQVNTKHNISLNWKEDPKTNNFFSATFGFILPVAYSYQPNLTIVIVGANQNLGKNRISLLISLLQGLAESQILAVIQDSEEKIMQDVANILTGDFIPNFENFAPDLQENVEVIKELRAQFQQEWKLLQCSVKRRIRGDVTAIFQMFQYLRNCHKEWRESTYFPKKVRKETMDGRREAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9L1</accession>
    <name>A0A6I9X9L1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106541386</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912289.1">
        <property type="nucleotide sequence ID" value="XM_014056814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9L1"/>
    <dbReference type="GeneID" id="106541386"/>
    <dbReference type="KEGG" id="tsr:106541386"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018241">
        <property type="entry name" value="Anion_exchange_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF15">
        <property type="entry name" value="ANION EXCHANGE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00220">
        <property type="entry name" value="ANION_EXCHANGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="129"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="227"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912289.1"/>
        </source>
    </evidence>
    <sequence length="500" mass="56808" checksum="067048491149A3B1" modified="2020-10-07" version="1">LSIAMGSILRQIPLAVLFGVFLYMGVTSLTGIQLYERLLLIFMPSKHHPDHIYVIKVKTWRMNLFTCIQLACLVLLWVVKSTVASLAFPFVLIMTVPLRRYLLPRFFHERELKAKADLGDVSKLPFLFLFCQFCQSQNIEYLTGRVWIGFWLIVFIIIIVAAEGSFLVRYITPFTQEIFAFLISLIFIYETFYKLYKIFAEHPLLRFYPPNLQRNLNTSILSADSTALDIRMQPNTALLSLILMLGTFFIAFFMRKFKNSRFLGGKVRRIIGDFGIPISILVMVLVDYTITDTYTQKLNVPSVLSVTAPDKRGWFIHPLGSQQPFPLWMMFASAIPALLVFILIFMETQITTLIVSKKERKLLKGSGFHLDLLLIGTMGGLCALLGLPWLTAATVRSITHVNALTVMSKAIAPGEKPKIKEVKEQRVTGVLIAGLVGLSIAMGSILRQIPLAVLFGVFLYMGVTSLTGIQLYERLLLIFMPSKHHPDHIYVIKVKTWRMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9L3</accession>
    <name>A0A6I9X9L3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetylserotonin O-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Hydroxyindole O-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106540010</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.4" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013910447.1">
        <property type="nucleotide sequence ID" value="XM_014054972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9L3"/>
    <dbReference type="GeneID" id="106540010"/>
    <dbReference type="KEGG" id="tsr:106540010"/>
    <dbReference type="OrthoDB" id="9045249at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017096">
        <property type="term" value="F:acetylserotonin O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030187">
        <property type="term" value="P:melatonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000358">
        <property type="entry name" value="Acetylserotonin O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0471">Melatonin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="10">
        <location>
            <begin position="77"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="11">
        <location>
            <begin position="210"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910447.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33095" checksum="5E44E17F82A2086E" modified="2020-10-07" version="1">MVLGKTRGHRIGRRKKTLISRLVPSCGRIFKMVSSSADESHYSEISHQVISEQVNARSSSRSLGKVPGGYTRQVCKSNRSKEELQTFSNFMKEIWPVVGREVLSAFDLSQFPLICDLGGNTGGLAKELISLYPRCTVTIFDLPKLAEASKNHYLPSGETRITFHAGDFLKDPIPETDLYILARTLHDWSDEKCLQLLRKVYQACKPELIISTACELGVFDLLRESREQLSSTTIAERLSTSPIGMQKLLEACVGLKFLTLECKDGKGNLILNKKNLEMIPSHFSCTPIQLMRSGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAG4</accession>
    <name>A0A6I9XAG4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein delta homolog 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Epidermal growth factor-like protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">DLK2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulates adipogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912589.1">
        <property type="nucleotide sequence ID" value="XM_014057114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAG4"/>
    <dbReference type="GeneID" id="106541612"/>
    <dbReference type="KEGG" id="tsr:106541612"/>
    <dbReference type="CTD" id="65989"/>
    <dbReference type="OrthoDB" id="430340at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000607">
        <property type="entry name" value="Protein delta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000118">
        <property type="entry name" value="protein delta homolog 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000263">
        <property type="entry name" value="Protein delta homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF38">
        <property type="entry name" value="DELTA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027077188" description="Protein delta homolog 2" evidence="15">
        <location>
            <begin position="28"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="92"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="132"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="213"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013912589.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="43575" checksum="0F20EDFC22D1A2AE" modified="2020-10-07" version="1">MLRSFCLQLMSLLWILAAHHHFIQVAGDDCSDHCNLAHGSCEKDGKCRCDPGWEGKYCEHCVRMPGCGHGTCHQPWQCICQTGWAGKFCDKDVHVCEHQSPCRNGAECVYDRDGEYSCLCLEGFHGKDCELKTGPCEKARFPCKNGALCHDDNGFASNYTCKCLAGFTGAHCEIDVNDCLMRPCANGATCLDGINRFSCQCQVGFEGRFCTINIDDCASQPCTNGAKCYDRINDFDCLCPEGFSGKTCQLQAPEATWITESQPDDKDHNRALGSTTSSYPWMTQSEPAKVIVTGKRITNYSEKSNDDGLLISVKEVVTQQDSGLSQSQLILVLVFGGITILLVLVTVLLLLKNWQKGRRRSQSPSQTARKLQDQECQVGMLSTILIEPRKTTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAT9</accession>
    <name>A0A6I9XAT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Alcohol dehydrogenase 1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106540717</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911421.1">
        <property type="nucleotide sequence ID" value="XM_014055946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAT9"/>
    <dbReference type="GeneID" id="106540717"/>
    <dbReference type="KEGG" id="tsr:106540717"/>
    <dbReference type="OrthoDB" id="417550at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004745">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042573">
        <property type="term" value="P:retinoic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042572">
        <property type="term" value="P:retinol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000067">
        <property type="entry name" value="alcohol dehydrogenase 1-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF8">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 6 (CLASS V)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="8">
        <location>
            <begin position="34"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="7">
        <location>
            <begin position="205"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013911421.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="30496" checksum="6AB5CBF5D8767D78" modified="2020-10-07" version="1">MATAGKVIKCKAAIIWEPGKAFSIEEIEVAPPKSHEIRVKMVATGICRTDDHVWKGFFPNIDYPVIGGHEGAGIVESIGPEVTCVKPGDKVIPLCVPQCGQCDCCLSPKANCCLKSHIYKAFQSVMPDGTSRFTCRGKQIHHFLWISTFSEYTVMPEVSIVRIDDDAPLDKVCLFGCGFSTGYGAAINCAKVDPGSSCAVFGLGGVGLSVIMGCKAAGASRIFGIDINKDKFIKAKELGATECFCPRDFEKPIHEVLVQKTGLGVDYTFEVIGRIDTEVCNLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBP1</accession>
    <name>A0A6I9XBP1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Junctophilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">JPH1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the junctophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908603.1">
        <property type="nucleotide sequence ID" value="XM_014053128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBP1"/>
    <dbReference type="GeneID" id="106538601"/>
    <dbReference type="KEGG" id="tsr:106538601"/>
    <dbReference type="CTD" id="56704"/>
    <dbReference type="OrthoDB" id="284854at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030314">
        <property type="term" value="C:junctional membrane complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000001">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000003">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000012">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017191">
        <property type="entry name" value="Junctophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085:SF6">
        <property type="entry name" value="JUNCTOPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037387">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="434"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="565"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="472"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="576"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013908603.1"/>
        </source>
    </evidence>
    <sequence length="657" mass="72053" checksum="1D1AEE7C0208F191" modified="2020-10-07" version="1">MTGGRFDFDDGGTYCGGWEDGKAHGHGICTGPKGQGEYAGSWAHGFEVVGTYTWPSGNTYHGYWAQGKRHGLGVETKGKWMYRGEWSHGFKGRYGVRQSLTSPARYEGTWSNGLQDGYGVETYGDGGTYQGQWTGGMRHGYGMRQSVPYGMATVIRSPLRTSLASLRSEQSNGTVLHDITADSPAGTRGGFVLNFHSDPDSVGSKKKGGLFRRGSLLGSMKLRKSESKSSISSKRSSVRSDAAMSRISSSDANSTISFGDMDCDYSPVEDHVDATTTETYMGEWKNDKRNGFGISERSNGMKYEGEWLNNKRHGYGCTMLPDGTKEEGKYKNNVLVRGIRKQLIPIRNTKTKEKVERALEGAQRAAAMARTKVEIAASRTAHARAKADTAEQASQSARQECDVARAVARELSPGFYQPGPDYIKQRFQEGEIKEKPEEKAQENPPTPKESPHFYRKGTTPPGSPEESHKHSSASSAKPSPAEQGRKHNSTPEARLIQEKKNVVSENIVPVVHKPVFSKPPAVKEEVMAKHHQKLSPRNNPKSKENGELHDHYHGYFVKTNPTLYPYELGEDEDHSNPSSSAVKPAASAQKPSPTRAGNQLDAKESLKKSESSAPKNPANNDLSSMENEISLGPNSIMIVMVMLLNVGLAILFVHFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBZ3</accession>
    <name>A0A6I9XBZ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase TRIM17-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106538656</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908698.1">
        <property type="nucleotide sequence ID" value="XM_014053223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBZ3"/>
    <dbReference type="GeneID" id="106538656"/>
    <dbReference type="KEGG" id="tsr:106538656"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908698.1"/>
        </source>
    </evidence>
    <sequence length="218" mass="25934" checksum="0E697AF57F5EA8F9" modified="2020-10-07" version="1">MAGKSQEQTIQEELTCSICYELFRNPVMLECMHHFCKECIEKYWNGCPRIATCPQCRQKCPSRSFHPNFIVSNIAEKVRRSASEEHRRKTKMELQKVLQVYQRKREKLLEMKRRNEENKECLVKTSRKLKSEIQAAFQHLHQILREEEGRILMEMATEEEQYMFRLENASLQLIEEISELKKSMDQMQRRLDNSEISSGLQVESLPVRYVSGKQTNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD75</accession>
    <name>A0A6I9XD75_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pumilio homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PUM1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911797.1">
        <property type="nucleotide sequence ID" value="XM_014056322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD75"/>
    <dbReference type="GeneID" id="106541004"/>
    <dbReference type="KEGG" id="tsr:106541004"/>
    <dbReference type="CTD" id="9698"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035196">
        <property type="term" value="P:miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF1">
        <property type="entry name" value="PUMILIO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PUM-HD" evidence="10">
        <location>
            <begin position="825"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="845"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="881"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="917"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="953"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="989"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="1025"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="1061"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="1100"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="24"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="233"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="485"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="610"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="739"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="485"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="510"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="625"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="760"/>
            <end position="772"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013911797.1"/>
        </source>
    </evidence>
    <sequence length="1183" mass="126166" checksum="9FB6C754F3A7EEB9" modified="2020-10-07" version="1">MSVACVLKRKAVLWQDSFSPHLKQHSQETANPNMPVVLTSGTGSQAQTQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGSYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLPGKKFWESDDSSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQGFHVNNEVNSVLSPRSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDAENDDNDKGDKKNKGTFDGDKLGDLKEEGDVMDKANGLPVQNGIDADAKDFSRTPGNCQNSANDVDLLGPNQNGSESLAQLASTNGAKPVEDFSNMESQSVPLDPMEHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTDPYAAGLAAAATLGPAVVPHQYYGVAPWGVYPASLFQQQAAAAAATNSSNQQTSQQTQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVNTGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTSGPFRPLGTQQPQPQQQPTSNLASSSFYSNNSLTSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSNTGSGSRRDSLTGSTDLYKRTSSNLTPIGHSFYNGLGFSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFPSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPPDQQNEMVRELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPEQTLPILEELHQHTDQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAMLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPAQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDX7</accession>
    <name>A0A6I9XDX7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium-independent sulfate anion transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Solute carrier family 26 member 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SLC26A11</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Sodium-independent anion exchanger mediating bicarbonate, chloride, sulfate and oxalate transport. Exhibits sodium-independent sulfate anion transporter activity that may cooperate with SLC26A2 to mediate DIDS-sensitive sulfate uptake into high endothelial venules endothelial cells (HEVEC). In the kidney, mediates chloride-bicarbonate exchange, facilitating V-ATPase-mediated acid secretion. May function as a chloride channel, playing an important role in moderating chloride homeostasis and neuronal activity in the cerebellum.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72363"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>oxalate(in) + chloride(out) = oxalate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72263"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sulfate(in) + H(+)(in) = sulfate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28574"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912072.1">
        <property type="nucleotide sequence ID" value="XM_014056597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDX7"/>
    <dbReference type="GeneID" id="106541219"/>
    <dbReference type="KEGG" id="tsr:106541219"/>
    <dbReference type="CTD" id="284129"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000013">
        <property type="entry name" value="Solute carrier family 26 member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="426"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="20">
        <location>
            <begin position="467"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013912072.1"/>
        </source>
    </evidence>
    <sequence length="591" mass="64887" checksum="0382FA385BEC5726" modified="2020-10-07" version="1">MSERNQERWEQCCRATAKRLPVLTWLPKYSLQWLQLDLMAGLTVGMTVVPQALAYAVVAGLPVQYGLYSSFMGCFIYCFLGTSKDVTLGPTAIMSLLVSSYAFHDPSLAILLSFLSGCIQVAMGLLHLGFILDFISYPVIKGFTSAAAVTIGFNQVKTLLGLQHIPKQFILQVYYTFYRISETRAGDAILGVCCLIVLMVLQQMKKSIPRAHLMETLPVRISRYIIWTTATARNAFVVLSAGLVAYSFQVTGSQPFSLTGTIPQGLPLFQPPPFSIITPNQTVDFKEMVEAIGPGLAVVPLMGLLETVAIAKAFASHNNYQIDSNQELLAMGFTNLLGSFVSSYPVTGSFGRTAVNAQTGVCTPAGGLITGALVLLSLAYLTSLFYYIPKAALAAVIICAVAPMFDARIFRTLWQVKRLDLVPLCVTFFVCFWEVQYGIIAGVTVSGILLLYPLARPRIKIVRDKAIVVKPENGLQFPAIDFLRDTVHKQALSEPQQRSVILDCSHINNIDYTVVLGLSELLQEFQLRELCLIFIGLQAHVLEIFQAADLKGFRNFSTLNDAERSLGELYVATEDMLSDNSSTLTTTGIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XE87</accession>
    <name>A0A6I9XE87_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">LIM domain-binding protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LDB1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the LDB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909428.1">
        <property type="nucleotide sequence ID" value="XM_014053953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XE87"/>
    <dbReference type="GeneID" id="106539213"/>
    <dbReference type="KEGG" id="tsr:106539213"/>
    <dbReference type="CTD" id="8861"/>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030274">
        <property type="term" value="F:LIM domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000063">
        <property type="entry name" value="LIM domain-binding protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041363">
        <property type="entry name" value="LID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17916">
        <property type="entry name" value="LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51957">
        <property type="entry name" value="LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LIM interaction" evidence="6">
        <location>
            <begin position="340"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="288"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="371"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="306"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909428.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="46972" checksum="D3D5FC8E509EE5A3" modified="2020-10-07" version="1">MSVGCACPGCSSKSFKLYSPKEPPNGNAFTPFHSGTMLDRDVGPTPMYPPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTITFCLEDGPKRYTIGRTLIPRYFRSIFEGGATELYYVLKHPKESFHNNFVSLDCDQCTMVTQHGKPMFTQVSVEGRLYLEFMFDDMMRIKTWHFSVRQHRELIPRSILAMHVSSEAQDPQMLDQLSKNITRCGLSNSTLNYLRLCVILEPMQELMSRHKTYSLSPRDCLKTCLFQKWQRMVAPPAEPARQQPSKRRKRKMSGGSTMSSGGGNTNNSNSKKKSPASTFALSSQVPDVMVVGEPTLMGGEFGDEDERLITRLENTQFDAANGIDDEDSFNNSPALGANSPWNSKPPSSQESKSENPTSQASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFJ5</accession>
    <name>A0A6I9XFJ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Oxysterol-binding protein-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23 24" type="primary">OSBPL3</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23 24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With VAPA, may regulate ER morphology. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion. Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3. Also binds 25-hydroxycholesterol and cholesterol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Interacts with RRAS. Interacts (phosphorylated form) with VAPA. Interacts with OSBPL6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium tip</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909823.1">
        <property type="nucleotide sequence ID" value="XM_014054348.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909824.1">
        <property type="nucleotide sequence ID" value="XM_014054349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFJ5"/>
    <dbReference type="GeneID" id="106539544"/>
    <dbReference type="KEGG" id="tsr:106539544"/>
    <dbReference type="CTD" id="26031"/>
    <dbReference type="OrthoDB" id="1854502at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13287">
        <property type="entry name" value="PH_ORP3_ORP6_ORP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000011">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000001">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000004">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041680">
        <property type="entry name" value="PH_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF15">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15409">
        <property type="entry name" value="PH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="21">
        <location>
            <begin position="50"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="463"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013909823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013909824.1"/>
        </source>
    </evidence>
    <sequence length="888" mass="101566" checksum="B4E5143D76EB2CC0" modified="2020-10-07" version="1">MTEEKNLGVSQSALSHSRSTSSCSSKQGSRQDSWEIIEGLRGVMSCTQEPQKQEGYLLKKRKWPLKGWHKRWFFLDKGILKYAKNPADTEKGKLHGCIDVGLSVMSVKKSTKCIDLDTEEHIYHLKAKSQEVFDEWVAKLRHHRMYRQNEISMFPYDINNHFFSASTSIADSVTGLLESTANKKRGSMTQQNSVQAGSSMSFPCLNESRIPTCLKSSEDMERCSKDLSHCQNYLLEMTQLLQSMEVLHRTYSAPAINIQQGLLFESPKKEKRTHRRWRSRALGKDAKGMLQVPAVLSAPLRLHASNPNLSTLDYGDEKMYSNGSEVTSEFSKLQEDLCHIAHKVYFTLKSAFSTISTEKEKLKQMFDHDSIASPSAQIIGLKNALSSAIAQNTDLKERLCRIHAESMLLDSAANANSSPDFVKESSEHQNRSLVHQISNESRLSITDSLTEFFDAQEVLLSASSSENEVSDDDSYASDISDNISEDNLSNDIENERQTLDCAPDGIVECYTRRRTRLPAPCPNTSNISLWNILRNNIGKDLSKVSMPVGLNEPLNTLQKLCEELEYSELLDKAAFTPNPFERMVYVAAFAVSAYASSYYRAGSKPFNPVLGETYECIREDKGFRFFSEQVSHHPAISACHAESDNFIFWQDIRWKNKFWGKSIEIVPIGTTHVILPGFKDHYEWNKVTSCIHNILNGQRWIEHYGEIIIKNLNDDSCHCKLTFVKTKYWNRSVHDIEGNVLDKNGKVVHRLFGKWHENIYCGTPSSPNCIWRANPMPKDYEQWYGFTQFALEINEFESISRPLLPPTDTRFRPDQRFLEEGNIEGAEIQKQRIEELQRARRKVLEESNIEHHPRFFRKSADDSWVSNGTYLELRKNPGFSMLDNPVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFZ9</accession>
    <name>A0A6I9XFZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GDP-D-mannose dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106542013</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013913086.1">
        <property type="nucleotide sequence ID" value="XM_014057611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFZ9"/>
    <dbReference type="GeneID" id="106542013"/>
    <dbReference type="KEGG" id="tsr:106542013"/>
    <dbReference type="OrthoDB" id="10253554at2759"/>
    <dbReference type="UniPathway" id="UPA00128">
        <property type="reaction ID" value="UER00190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000924">
        <property type="entry name" value="GDP-mannose 4,6 dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715:SF1">
        <property type="entry name" value="GDP-MANNOSE 4,6 DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="GDP-MANNOSE 4,6-DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="7">
        <location>
            <begin position="8"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013913086.1"/>
        </source>
    </evidence>
    <sequence length="88" mass="10025" checksum="4E5F27CE15D019E1" modified="2020-10-07" version="1">MPYTSKRDGSYLAEFLLEKGYEVHGIVRRSSSFNTGRIEHLYKNPQAHIEGNMKLHYGDLTDSTCLVKIINEVKPSEIYNLGAQSHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGJ7</accession>
    <name>A0A6I9XGJ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MPST</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913251.1">
        <property type="nucleotide sequence ID" value="XM_014057776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGJ7"/>
    <dbReference type="GeneID" id="106542129"/>
    <dbReference type="KEGG" id="tsr:106542129"/>
    <dbReference type="CTD" id="4357"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000001">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000008">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008557">
        <property type="entry name" value="PRK11493.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF25">
        <property type="entry name" value="3-MERCAPTOPYRUVATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="25"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="174"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913251.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="32300" checksum="81E2683338128547" modified="2020-10-07" version="1">MSQQLLYRALVTAKWVSEVIKTPQTGSSVKLLDASWYLPKMKRNPQREFEDRHIPGAAFFDIDHCSDRTSPYDHMLPSATDFAEYVGKLGVSNDCHVVVYDASDQGLFSAPRVWWMFRAFGHEAVSLLNGGLKNWMKEGYPVTSGKSHPAPAEFHASLDKSMVKTYEQVKENIDSRQFQVVDARSAGRYAGTEPEPREGIEPGHMPGSVNIPFTEFLTETGLEKSSETIQSLFQEKKVDLSKPLAATCGSGVTACHVALGAYLCGKPDVAIYDGAWVEWFMRAQPEDIISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ94</accession>
    <name>A0A6I9XJ94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MOB kinase activator 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Mob2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mps one binder kinase activator-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MOB2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Stimulates the autophosphorylation and kinase activity of STK38 and STK38L.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Binds STK38 and STK38L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MOB1/phocein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914106.1">
        <property type="nucleotide sequence ID" value="XM_014058631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJ94"/>
    <dbReference type="GeneID" id="106542793"/>
    <dbReference type="KEGG" id="tsr:106542793"/>
    <dbReference type="CTD" id="81532"/>
    <dbReference type="OrthoDB" id="8170117at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000003">
        <property type="entry name" value="MOB kinase activator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="241"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013914106.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="30993" checksum="E6514B622C4A2C12" modified="2020-10-07" version="1">MVGDHCNHSSERPVLSQTPKTGISCKMVLQAVGKVLRKSKGKPNGKKPATEEKKLYLEPEYTKSRITDFEFKELVILPREIDLNEWLASNTTTFFHHINLQYSTISEFCTGESCQTMAVCNTQYYWYDERGKKIKCTAPQYVDFVMSSVQKLVTDEDVFPTKYGKEFPNSFESLVKKICKYLFHVLAHIYSSHFKETLALELHGHLNTLYVHFILFIREFNLVDPKETTIMDDLTEILCSSSGSSSSNGNGNGSGGGGSGSSSSSSAVAQNHVKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK91</accession>
    <name>A0A6I9XK91_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">LOC106542987</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013914347.1">
        <property type="nucleotide sequence ID" value="XM_014058872.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914348.1">
        <property type="nucleotide sequence ID" value="XM_014058873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK91"/>
    <dbReference type="GeneID" id="106542987"/>
    <dbReference type="KEGG" id="tsr:106542987"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000008">
        <property type="entry name" value="histidine--tRNA ligase, cytoplasmic isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="63"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="332"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914348.1"/>
        </source>
    </evidence>
    <sequence length="509" mass="58376" checksum="F4D02BEED9FB6F9C" modified="2020-10-07" version="1">MHWITVLRTASFRTQHLPVQFSILRLFSSHLPNQATENSEKQLSHQQNAGTSKGNLILKVPKGTRDFSPKQMAIREKILSTIISCFKRHGAVTIDTPVFELKELLQENYGEEAKLIYDLEDQGGEMLSLRYDLTVPFARFLAMNKIKRCKRYHIAKVYRRDSPSIKRGRFREFIQCDFDIAGQYDPMIPDAECLKVIHEILTAMQVGDFLIKINDRRILQGIFTICGIPEIKFERICSTLDKLDKISWEEVKNEMILEDGISPECVNRISEYVQLHGGQCLIEKLLKDPKLAQNRLAMEGLEDMKLLFEYLTLFGIVDQVSLHLSLARGLAYYTGVIYEAVLLCQKHGHLNESLSVSSVAGGGRYDELVAKFGSKRHKVPCVGLSIGVERIFSIIEQNNEMAKEKIRTTETQVLVATPQKNFLTIRMKLISELWNSGIKAEMLYKKNPRLLEQLQYCEETGIPLAIIIGEQELNDGVVKLRDVVTRKEVNVPQEHFIEEIKRRLEKHNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLW6</accession>
    <name>A0A6I9XLW6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger and BTB domain-containing protein 42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZBTB42</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the krueppel C2H2-type zinc-finger protein family. ZBTB18 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911828.1">
        <property type="nucleotide sequence ID" value="XM_014056353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLW6"/>
    <dbReference type="GeneID" id="106541028"/>
    <dbReference type="KEGG" id="tsr:106541028"/>
    <dbReference type="CTD" id="100128927"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18325">
        <property type="entry name" value="BTB_POZ_ZBTB18_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000114">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF20">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="444"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013911828.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="52827" checksum="65D9DD344C854962" modified="2020-10-07" version="1">MEFPDHSRQLLQCLSQQRHQGFLCDCTVLVGDAQFRAHRAVLASCSMYFHLFYRDQLDKRDIVHLNSDIVTAPAFGLLLEFMYEGKLEFNSLPVEDVLAAASYLHMYDIVKVCKGKLKDKELCGDEKMNGSGISLERDMRVLDEGVPLGRELSPGNKQKLIASEYERSAGKEKDSSYLGWSSEFVSINSALAEAELDTVAAEKTRANVCSSVGPLSQRSLNHSLASSGIECALDLSFKSVAGKDSFHPSYVFGQLASSGQQQSSKPLIKDEPESLSDGEESEVRCLENQHLGNSAKSLMTGLGNVFAGNGNSQEPEEDLDHEREVSEDDMDSSDIPSLGVLVPPGHICICPLCSKVFPSPHVLQLHLSSHFRDKDVSRTRLSPDGSVPTCTLCGKTFSCMYTLKRHERTHSGEKPYTCGQCGKSFQYSHNLSRHAVVHTREKPHGCKWCERRFTQSGDLYRHIRKFHCGLVKSLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMP0</accession>
    <name>A0A6I9XMP0_9SAUR</name>
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        <submittedName>
            <fullName evidence="5">A disintegrin and metalloproteinase with thrombospondin motifs 17-like</fullName>
        </submittedName>
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        <name evidence="5" type="primary">LOC106545227</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="TSP1_rpt"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013917194.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="16857" checksum="A043447C9566A8FF" modified="2020-10-07" version="1">MCTNPQGKCDAATRPRDEEECEDHTGCYEWKTGEWSKCSSTCGKGLQSRVVQCMHKVTGRHGNECPTFSKLAAYRQCHQQACNEKINVNTITSPRLAALTYKCTGDQWAVYCRVIREKNLCQDMRWYQRCCQTCRDFYASKMQQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMZ8</accession>
    <name>A0A6I9XMZ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calumenin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106543733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915286.1">
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    <dbReference type="KEGG" id="tsr:106543733"/>
    <dbReference type="OrthoDB" id="293868at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026898155" evidence="2">
        <location>
            <begin position="22"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915286.1"/>
        </source>
    </evidence>
    <sequence length="76" mass="8492" checksum="039B52C6748A2C2E" modified="2020-10-07" version="1">MVLGVQQVVGCLALCAFCAQSKPTEKKDRVHHDSQLSDKVHDDGQNFDYDHDAFLGVDEAKTFDQLTPEESKERLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNC8</accession>
    <name>A0A6I9XNC8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glia-derived nexin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SERPINE2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915447.1">
        <property type="nucleotide sequence ID" value="XM_014059972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNC8"/>
    <dbReference type="GeneID" id="106543869"/>
    <dbReference type="KEGG" id="tsr:106543869"/>
    <dbReference type="CTD" id="5270"/>
    <dbReference type="OrthoDB" id="671595at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044706">
        <property type="term" value="P:multi-multicellular organism process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030195">
        <property type="term" value="P:negative regulation of blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010757">
        <property type="term" value="P:negative regulation of plasminogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033363">
        <property type="term" value="P:secretory granule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19573">
        <property type="entry name" value="serpinE2_GDN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000006">
        <property type="entry name" value="Plasminogen activator inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000035">
        <property type="entry name" value="Serine protease inhibitor (serpin) 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.310.10:FF:000001">
        <property type="entry name" value="Serpin family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF48">
        <property type="entry name" value="GLIA-DERIVED NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027037522" evidence="6">
        <location>
            <begin position="21"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="7">
        <location>
            <begin position="35"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013915447.1"/>
        </source>
    </evidence>
    <sequence length="396" mass="44032" checksum="1465BB28CEFB2213" modified="2020-10-07" version="1">MYWHFPLLFAALTSTYVCFPQNHLSLEELGSDIGIQVFNQLVKTRAGDNVVVSPHGIASVLGMLQLGADGKTKKELTTVMRYSVNGVGKVLKKINKAIVSRRNKDIVKIANIVFANSGLKMETPFVSRNSEVFQCPVKNINFKDPNAASDTINLWVKNKTEGMIDSVLTPGDIDSVLTRLVVINAIYFKGLWRSRFQPENTKMRTFNEGDGKAYQVPMLAQLSVFRCGTTSTPNDLWYNTIELPYHGESISMLIALPAQSSTPLSAIIPHISTKTIQSWMTTMVQKRVQVILPKFTAEAETDLKGPLKELGIRDMFHKSKANFAKITRSENIHVSQLLQKAKIEVTEDGTKASAATTAIVTARSSPPWFVVDRPFLFFIRHNPTGAVLFMGQITKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP50</accession>
    <name>A0A6I9XP50_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">FYVE, RhoGEF and PH domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">FGD2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915641.1">
        <property type="nucleotide sequence ID" value="XM_014060166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP50"/>
    <dbReference type="GeneID" id="106544030"/>
    <dbReference type="KEGG" id="tsr:106544030"/>
    <dbReference type="CTD" id="221472"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046847">
        <property type="term" value="P:filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15741">
        <property type="entry name" value="FYVE_FGD1_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13236">
        <property type="entry name" value="PH2_FGD1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000061">
        <property type="entry name" value="FYVE, RhoGEF and PH domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000013">
        <property type="entry name" value="FYVE, RhoGEF and PH domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035941">
        <property type="entry name" value="FGD1-4_PH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF82">
        <property type="entry name" value="FYVE, RHOGEF AND PH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="15">
        <location>
            <begin position="135"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="352"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="16">
        <location>
            <begin position="492"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="578"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="457"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013915641.1"/>
        </source>
    </evidence>
    <sequence length="688" mass="79475" checksum="9DFEBC3942F2A14E" modified="2020-10-07" version="1">MMEEKQKLPETSVVNLVNIFENFRTCCSPEKEQILPQLRFQLTTPRPMAPSMLPPVKCEKAMGDIKKAAVIQPGQVHCSAESLEQQGQAPKMLPVKCLSSFRGKITVSDWRAKQASDSQPYFIMQSGSSKNQEPEEKKIALELLETEQAYVVRLHLLDQVFFSELMKEARTTKAFPEDVVKMIFSNISSIYQFHSQFFLPELKKRMEDWDSNPRIGDIIQKVAPFLKMYSEYVKNFDKAMHLITVWSEKALPFQELISNIQKQEVSANLTLQHHMLEPIQRIPRYELLLKDYIQKLPLESPDKEDAQKALEMIFTAAKHSNAAIAEMERLQNLWDVYHRLGCDDDIVDPSNEFIKEGPIQKISFRHNNTSERYLFLFNNLLLYCVPKVIQMGAEFQVRHRIDVNDMKVRELNDVQFPHAFLVSGKQRTLELQARSQEEMDAWLKTFQSAIDKKEKRSETFKAASQGQEAETQDSSQGESQELGKWAPQWIRDKLVTMCMRCREPFNPITRRRHHCRACGYVVCARCSEYKVELQYDKNGLKRVCAECYGFLTGNLLPVEQHGKHKGILEKESAEISGQSLMCSFLQLMDKNGKGSMRGWFVIPRDDPLVLYIYAAPQDVKACASIPLLGYHIKEPYQNNPRHIFQLVQSKQTHTLVAETEELKQRWMKAIEQSARGGEEEDDSCDDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPG0</accession>
    <name>A0A6I9XPG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Core histone macro-H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">LOC106545760</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Variant histone H2A which replaces conventional H2A in a subset of nucleosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917889.1">
        <property type="nucleotide sequence ID" value="XM_014062414.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917890.1">
        <property type="nucleotide sequence ID" value="XM_014062415.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917891.1">
        <property type="nucleotide sequence ID" value="XM_014062416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPG0"/>
    <dbReference type="GeneID" id="106545760"/>
    <dbReference type="KEGG" id="tsr:106545760"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02904">
        <property type="entry name" value="Macro_H2A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000013">
        <property type="entry name" value="Core histone macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.220.10:FF:000002">
        <property type="entry name" value="Core histone macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021171">
        <property type="entry name" value="Core_histone_macro-H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035796">
        <property type="entry name" value="Macro_H2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037942">
        <property type="entry name" value="Core_histone_macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="12">
        <location>
            <begin position="184"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="116"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate" evidence="9">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="9">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037942-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013917889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013917890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013917891.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="40269" checksum="5B6A6AFA2CFFA5DA" modified="2020-10-07" version="1">MSARGGKKKMSKISRSSRAGVIFPVGRMMRYLKKGTYKYRIGMGAPVYMAAVIEYLAAEILELAGNAARDNKKGRIAPRHILLAVANDEELNQLLKGVTIASGGVLPRIHPELLAKKRGAKSKSDTILSPTPEKRGRKPLGVKKSGKKAKTGKARTPKKNKQKENEKEGASNSTADDGPGDGFTILSSKSLVPGQKLSLTQSDINHIGSMKVEGIVHPTTGEIDLKEEVGKALEKAGGKEFLETVKELRKSQGPLEVTEAALGQATGLAAKFVIHCHIPQWGSDKCEEQLEETIKNCLTTAEDKKLKSVAFPPFPSGRNCFPKQTAAQVTLKAISSHFDDSSPSSLKNIYFLLFDSESIGIYVQEMAKLDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPS5</accession>
    <name>A0A6I9XPS5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Neutral amino acid transporter 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Solute carrier family 38 member 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">SLC38A9</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-asparagine(out) = L-asparagine(in)</text>
            <dbReference type="Rhea" id="RHEA:73423"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamine(out) = L-glutamine(in)</text>
            <dbReference type="Rhea" id="RHEA:73419"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-leucine(in) = L-leucine(out)</text>
            <dbReference type="Rhea" id="RHEA:73011"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosine(in) = L-tyrosine(out)</text>
            <dbReference type="Rhea" id="RHEA:68572"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the amino acid/polyamine transporter 2 family. SLC38A9 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915816.1">
        <property type="nucleotide sequence ID" value="XM_014060341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPS5"/>
    <dbReference type="GeneID" id="106544157"/>
    <dbReference type="KEGG" id="tsr:106544157"/>
    <dbReference type="CTD" id="153129"/>
    <dbReference type="OrthoDB" id="294730at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF244">
        <property type="entry name" value="NEUTRAL AMINO ACID TRANSPORTER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="471"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="502"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="22">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="22">
        <location>
            <begin position="294"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013915816.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="62854" checksum="27A2B5BB8E97A9CB" modified="2020-10-07" version="1">MEEDATPLLIPVGGDENDLGILNIQFSPEDLKSKRPFHVEPTNIVRVNDDAQRVTDEASAMNKRIHYYNRLTSPSDRALVAPDHVLPAPEEIYVYSPLGTACKIDSSTDGSGKNSSIVTIFMIWNTMMGTSILSIPWGIKQAGFTTGICIIPLMGMLTLYCCYRVVKSRSMIPSVDTSTWEFPDVCKYYFGSFGQWSSLLFSLVSLIGAMVVYWVLMTNFLFNTGKCIYNYIHDINVTDIVPGTNGSSRVICPSDSNHKVPGNETFEFTSIGAVGFEEFEKWWDKSKTVPFYLTIILLLLNFKSPSFFAKFNILGTVSVIYLVILVTVKAALLGFHLEFDWTATNIYFVPEFRIFFPQLTGVLTLAFFIHNCVITLLKNNRNQEKNVRDLSIAYFLVSFTYLYVGVLIFASFPSPPLSKECIEQNFLDNFPSNDIMSFLARIFLLFQMVTVYPLLGYLARVQFLGHVFGNVYPSFFHVLVSNIIIVGIGVTVARFYPNIGGIIRYSGATCGLAFVFVYPSIIYIISLHRENQLTWFALISHFLIILLGVANLMAQFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPW9</accession>
    <name>A0A6I9XPW9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fanconi anemia group D2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FANCD2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Fanconi anemia protein FANCD2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912753.1">
        <property type="nucleotide sequence ID" value="XM_014057278.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541745"/>
    <dbReference type="KEGG" id="tsr:106541745"/>
    <dbReference type="CTD" id="2177"/>
    <dbReference type="OrthoDB" id="27031at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11721">
        <property type="entry name" value="FANCD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029448">
        <property type="entry name" value="FANCD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32086">
        <property type="entry name" value="FANCONI ANEMIA GROUP D2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32086:SF0">
        <property type="entry name" value="FANCONI ANEMIA GROUP D2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14631">
        <property type="entry name" value="FancD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1401"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1413"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1423"/>
            <end position="1445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912753.1"/>
        </source>
    </evidence>
    <sequence length="1445" mass="163021" checksum="74E730B1F141D105" modified="2020-10-07" version="1">MASKRKLFKPHVNSENLIEELPKTKRPRISGKKKSVLQDNDIENNNAFGHLLKTAGITLKAGEKQNEIAVDGAIFQKKLHQALRKHPSYPQIVAEFISGLDSHIENRDQFRNCLLPCIPAQNQETNDAVHSYCESLIKLLLGIELLQPAVIKLLFEKLPEFFQESVGSDGLNLPRLIVNQLKWLDRIVDNKDLVSMIMQMVYVSPPSIQHDIITSLPEILEDSQHSEIAKQLSSLLKENIGLTVPVLDALSCLNLDIELLSEIRQSVMISVSAVKVEDLPVIVKFILHSMKATEATEVISDLRKKLNLESCAALPSIQASQSKLSSKGQSSLSASQMSINKDSFKLLFDVLKSTLKFQKCVLEGWIKAIENIPSASDHKVLDMIMLLIIYATSTKNKKQIERLLKSKIQSGCLREQLLQNAFRYHSLVMRDLFPAILSVAESFVNSVDPCVVSFGSCMYKQAFMAFDSYCQQEIVGALVTHVCGGNKIEVDVSLDTLTDLVSVHTSSVVPYVVFLKSILDYMDNLGPQQIRKLFHILSMLAFSQGHRNSHIQDDMHMVIRKQLSSTIPKYKRIGIIGAVTMVGSMAAKRSKENGKLLEREQLSKEQHRQITTLLELVFSCCGQVPHALALYYDELANLIQKGNLDLQVLAWIKDYVVQDFESDFVTDLHQIEDSTFPFSVKALYGLEDGESQEGIAINLLPLLSQSFSTNLEVTAAESKDKRLVSPVCLSPFFRMLRLCMSELNKGSMEDIDALLVCPLYLTNLEVAEKIESLSKEEREFLCSLLFHAVNWFREVVNAFCREEDANIKGKVLTRLQNITELQCTLEKCLAASPGYIPPLANFDSETLEAAPAFNTISVSKKKNKGQKRLKSDSSKKSFGDNSQVDENLEIIQAEAETSQQEKSSSPEKDPATPLIQLQNYSAYFRELDLEVFTILHCGLLTKSVLDSEMHTKIREVVQLEPTELLFLLEDLYRKMEHMLIPAPARRLPFVKGKGNKNAGFSHLCQSTSQKVIEYAAELLKPLCNHIENMHNYFQTLITENHGVIDGPGVNIQEHQLMSSCYQRLLQIFHFLFAWNGFSQHENHGLLKASLDILACRLKPGDRELPLEDLLSQSFQYLLNFQHSIPNIFCALNLTQLLLVIAEKSVANPKSKEIVSMTKGFLCQSWMQPSGTRQKGSQFNDALHALLTIYMEYTQDILKEIEDISSTGVPELIRSSKDGYSSTYPTLTRQTFPIFFRVMMTKLETSIRSLSAGKSDSSEIQLQKLLHWNLAVRDFHILVNLVKVFDSRPVLSVCLKYGRLFIETFLKSGMPLLDYSFKKHREDVQSLLKTLQLSTRQLHHICGHSKIHQDTGLTSHVPLLKKSLELFVYRVKAMLALNHCQEAFWVGILKNRDLQGEEILSQASQVTEAEETEMPTHSRTDPGEEKEDSETEDTVDTNDSDEDSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ04</accession>
    <name>A0A6I9XQ04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17 18">Pro-opiomelanocortin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17 18" type="primary">POMC</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17 18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Endogenous opiate.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Stimulates the adrenal glands to release cortisol.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the POMC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912782.1">
        <property type="nucleotide sequence ID" value="XM_014057307.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912783.1">
        <property type="nucleotide sequence ID" value="XM_014057308.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912784.1">
        <property type="nucleotide sequence ID" value="XM_014057309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ04"/>
    <dbReference type="GeneID" id="106541774"/>
    <dbReference type="KEGG" id="tsr:106541774"/>
    <dbReference type="CTD" id="5443"/>
    <dbReference type="OrthoDB" id="8962839at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000852">
        <property type="term" value="P:regulation of corticosterone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013531">
        <property type="entry name" value="Mcrtin_ACTH_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013593">
        <property type="entry name" value="Melanocortin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013532">
        <property type="entry name" value="Opioid_neuropept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001941">
        <property type="entry name" value="PMOC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050878">
        <property type="entry name" value="POMC-derived_peptides"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11416">
        <property type="entry name" value="PRO-OPIOMELANOCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11416:SF7">
        <property type="entry name" value="PRO-OPIOMELANOCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00976">
        <property type="entry name" value="ACTH_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08384">
        <property type="entry name" value="NPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08035">
        <property type="entry name" value="Op_neuropeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00383">
        <property type="entry name" value="MELANOCORTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01363">
        <property type="entry name" value="ACTH_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01364">
        <property type="entry name" value="NPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01365">
        <property type="entry name" value="Op_neuropeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-0257">Endorphin</keyword>
    <keyword evidence="7" id="KW-0372">Hormone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636605" evidence="11">
        <location>
            <begin position="24"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Pro-opiomelanocortin N-terminal" evidence="13">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Pro-opiomelanocortin/corticotropin ACTH central region" evidence="12">
        <location>
            <begin position="134"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Pro-opiomelanocortin/corticotropin ACTH central region" evidence="12">
        <location>
            <begin position="214"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Opiodes neuropeptide" evidence="14">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="89"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013912782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013912783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013912784.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="30354" checksum="22A67F8191149D72" modified="2020-10-07" version="1">MLNSQWSSLWAIMGVLLIHSVAGVHSLCWDNSWCWDMDTEVKMMECFRACRTGLSNESPVYPGNGHMQPLSEDIQKNIPWKKFGKSNSLGNKIKNPSGDSGADLFSASSETQESWEGDSESHQESREKQEGKRSYAMEHFRWGKPVGRKRRPIRVNPNGAADESEESYPEEFRRDLSWEMQYPELDSPEEKESSETIKMDKEKQLRKIKNDNTAYKMHHFRWNAPALSKKKRYGGFMTSEHSHTPLVNLFKNAIIKTAYKKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ54</accession>
    <name>A0A6I9XQ54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DDX55</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013915931.1">
        <property type="nucleotide sequence ID" value="XM_014060456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ54"/>
    <dbReference type="GeneID" id="106544234"/>
    <dbReference type="KEGG" id="tsr:106544234"/>
    <dbReference type="CTD" id="57696"/>
    <dbReference type="OrthoDB" id="7396459at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001022">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026782418" description="ATP-dependent RNA helicase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="153"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013915931.1"/>
        </source>
    </evidence>
    <sequence length="401" mass="45360" checksum="FDFFA56D388A75E9" modified="2020-10-07" version="1">MLLLLLYFFFSSQCLLIGGKNPMEDVERFKERGGNILVATPGRLEDMFRRKSEGLDLAKAVKSLDILVLDEADRLLDMGFEASLNTILDFLPKQRRTGLFSATQTQEVENLVRAGLRNPVRISVKEKGMAASNTQKTPTRLQNYYMICKADEKFNQLVHFLRQHKPEKHLLFFSTCACVEYYGKALESLIKNVKIMCIHGKMKHKRNRIFTEFRKLPSGILVCTDVMARGIDIPEVNWVLQYDPPSSASAFVHRCGRTARIGHLGSALVFLLPMEEAYISFLAINQKCPMEELNPQRNVVDVLPKLKSLNLADRAVYEKGMKAFVSCVQAYAKHECNLIFRIKGELAWLENGSDVDNEDMEELLNDTRLLKKLKRGKISEKEFEKTLLGGGKGGSGSDSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQE6</accession>
    <name>A0A6I9XQE6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mucin-3B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106546035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918269.1">
        <property type="nucleotide sequence ID" value="XM_014062794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQE6"/>
    <dbReference type="GeneID" id="106546035"/>
    <dbReference type="KEGG" id="tsr:106546035"/>
    <dbReference type="OrthoDB" id="7493297at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053311">
        <property type="entry name" value="Mucosal_Integrity_Assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37999">
        <property type="entry name" value="MUCIN-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37999:SF2">
        <property type="entry name" value="MUCIN-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026805199" evidence="3">
        <location>
            <begin position="18"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="4">
        <location>
            <begin position="57"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013918269.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="35519" checksum="2494200AA2F2D32E" modified="2020-10-07" version="1">MVTGALFLFFLVPCVEAELCYNGGIFDGIKCICDENLYYGPKCEFPLDEIPVIQLSVTVTVDAQVRVTNKEYNKSLEDLNSNYYREIQTQFKKQMKDVYGAVPGYKDVEILRMKNGSIIVEHKVISQVLTENNLELIQRIIKIIIEAVEHSLKDLHTEGKCIDPSGPELCFVPLPNSALIPSFSLNDTCKNVSDTKYADYYYPYIRNGTVRCITRCSSGTPDSMNCFNGVCRLSEIGPHCICNNLDVFWYLDSYCRLPIQKTVLGLSLALAVFFVLSIILVICLIKAKRKNSRNRRSDGANCDKIGQRNPIFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR04</accession>
    <name>A0A6I9XR04_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Synaptotagmin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SYT1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="13">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Chromaffin granule membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">Secretory vesicle</location>
            <location evidence="1 13">Synaptic vesicle membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916502.1">
        <property type="nucleotide sequence ID" value="XM_014061027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR04"/>
    <dbReference type="GeneID" id="106544680"/>
    <dbReference type="KEGG" id="tsr:106544680"/>
    <dbReference type="CTD" id="6857"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042584">
        <property type="term" value="C:chromaffin granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08402">
        <property type="entry name" value="C2B_Synaptotagmin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21963">
        <property type="entry name" value="Syt1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000007">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000016">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF239">
        <property type="entry name" value="SYNAPTOTAGMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="142"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="273"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013916502.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="47447" checksum="73525238F1542ABB" modified="2020-10-07" version="1">MAKESQHQALAALPGTTVATAIANNATEPVIPGGGKGKEDAFSKLKEKFMNELTKIPLPPWALIAIAIVAVLLVLTCCFCLCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKNLKDQDDDDAETGLTDGEDKEEAKEEEKLGKIQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKYETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEYKVAMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQQEEEVDAMLAVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRR7</accession>
    <name>A0A6I9XRR7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">LIM/homeobox protein Lhx9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106544880</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916757.1">
        <property type="nucleotide sequence ID" value="XM_014061282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRR7"/>
    <dbReference type="GeneID" id="106544880"/>
    <dbReference type="KEGG" id="tsr:106544880"/>
    <dbReference type="OrthoDB" id="9990008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000027">
        <property type="entry name" value="LIM/homeobox protein Lhx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF95">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN LHX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="48"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="50"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013916757.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="19957" checksum="3902BD7BFD0175FF" modified="2020-10-07" version="1">MDTEVEHIAYFQLSNVVVVVCLLFSQTGCNENEADHLDRDQQPYPPSQKTKRMRTSFKHHQLRTMKSYFAINHNPDAKDLKQLAQKTGLTKRVLQVWFQNARAKFRRNLLRQENGGVDKADGTSLPAPPSADSGALTPPGTATTLTDLTNPTITVVSSVTSNMDSHDSASPSQTTLTNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTH7</accession>
    <name>A0A6I9XTH7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106545130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917066.1">
        <property type="nucleotide sequence ID" value="XM_014061591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTH7"/>
    <dbReference type="GeneID" id="106545130"/>
    <dbReference type="KEGG" id="tsr:106545130"/>
    <dbReference type="OrthoDB" id="6621890at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013917066.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="27678" checksum="10F4F2CFB1A461FE" modified="2020-10-07" version="1">MKAQSLSVPIQAEGGGPQGRPRDGVPWLSPLQDAQVEFGFGIASHLLQLGSGGPQAGVGIPASPPAVPLHEMYQELYYFFGDSLATRFGTGSGLCPAGPMPASPPKTTDTKKEQPAFGTGDAVRKMPQLTASATVSPRDEESPRPDFSASRPALHRSASLARSRSTLRQQGHRPVARDVFQKCRFSLQGAPHFSFVIAGRQVEHRCQGRLFQTCFPQHHGPTHISRYAQKLPMIGRTSKPAKKWSHSDYSLYLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUU5</accession>
    <name>A0A6I9XUU5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ADP-ribosylation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ARF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908450.1">
        <property type="nucleotide sequence ID" value="XM_014052975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUU5"/>
    <dbReference type="GeneID" id="106538481"/>
    <dbReference type="KEGG" id="tsr:106538481"/>
    <dbReference type="CTD" id="375"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="69"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013908450.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="14128" checksum="7AEC4FDEA8CCBF6A" modified="2020-10-07" version="1">MEMVALSQDLLQLEEPLARSYLTIPVSLPGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVH8</accession>
    <name>A0A6I9XVH8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Vesicle-associated membrane protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106545688</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917801.1">
        <property type="nucleotide sequence ID" value="XM_014062326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVH8"/>
    <dbReference type="GeneID" id="106545688"/>
    <dbReference type="KEGG" id="tsr:106545688"/>
    <dbReference type="OrthoDB" id="10042941at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000013">
        <property type="entry name" value="Vesicle-associated membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013917801.1"/>
        </source>
    </evidence>
    <sequence length="114" mass="12801" checksum="0ABD044AFDB7A895" modified="2020-10-07" version="1">MSAPNPFQGVSSASSPGVPPQSNMTSNRRLQQTQAQVNEVVDIMRVNVDKVLERDQKLSELDNRADALQAGASQFETNAAKLKRKYWWKNCKMMIILGVLCTIILIIIIIYFCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW46</accession>
    <name>A0A6I9XW46_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">CD37</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908780.1">
        <property type="nucleotide sequence ID" value="XM_014053305.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908781.1">
        <property type="nucleotide sequence ID" value="XM_014053306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW46"/>
    <dbReference type="GeneID" id="106538718"/>
    <dbReference type="KEGG" id="tsr:106538718"/>
    <dbReference type="CTD" id="951"/>
    <dbReference type="OrthoDB" id="438211at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF263">
        <property type="entry name" value="LEUKOCYTE ANTIGEN CD37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013908780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908781.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29283" checksum="BA56C6DB5808AD08" modified="2020-10-07" version="1">MSHKGCLSVTKYFLFLFNVFFFILGSLVFSFGLWILFDQNNFASTLGSSYYALKIWSYVFSGVGILTMFLGFLGCLGSLKEVKCMLGLYFAFLLLLFVSQVIIAILIQTHSNTITSTVAGRVKTIIAGYGTNTSLSDGDSWDFVQEQFQCCGWKGWADWMENSRVRNASNKEYPCSCQNTSALHSANNGTTDKPALFCRAEAPLQINNRGCRESVQTWLNNNIISIVGICLGIALLELFLMMVSMFLCRTIGPNYDKLARYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWF0</accession>
    <name>A0A6I9XWF0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106547787</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920519.1">
        <property type="nucleotide sequence ID" value="XM_014065044.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547787"/>
    <dbReference type="KEGG" id="tsr:106547787"/>
    <dbReference type="OrthoDB" id="5967130at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000410">
        <property type="entry name" value="Olfactory receptor 1348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013920519.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23861" checksum="702ED6C7BF24F111" modified="2020-10-07" version="1">MEEANQTNVSYFILLGFTTTAELQILLFVLFLIAYLMILMENLIIITVIWINNNLHKPMYLFLCNLSFLEIWYITVIVPKMLGDFLSQDKRISFQGCLTQLYFFVTFVCSEYILLAVMAYDRYLAICYPLRYPVLMNNNFCVQLSAGCWICGLLTSMIKLSFIGXIQSNKLVSVLYTVIVPLLNPLIYCLRNKEINCALRKTFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXA9</accession>
    <name>A0A6I9XXA9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Chromodomain-helicase-DNA-binding protein 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CHD6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918441.1">
        <property type="nucleotide sequence ID" value="XM_014062966.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546177"/>
    <dbReference type="KEGG" id="tsr:106546177"/>
    <dbReference type="CTD" id="84181"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18668">
        <property type="entry name" value="CD1_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18663">
        <property type="entry name" value="CD2_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000184">
        <property type="entry name" value="Chromodomain helicase DNA binding protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000003">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000015">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006576">
        <property type="entry name" value="BRK_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037259">
        <property type="entry name" value="BRK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051493">
        <property type="entry name" value="CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056342">
        <property type="entry name" value="HTH_CHD6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850:SF1">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07533">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23078">
        <property type="entry name" value="HTH_CHD6-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00592">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160481">
        <property type="entry name" value="BRK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="291"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="375"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="473"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="787"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="86"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1166"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1329"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1862"/>
            <end position="1907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2001"/>
            <end position="2069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2311"/>
            <end position="2341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2580"/>
            <end position="2634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2678"/>
            <end position="2749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="126"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1166"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1333"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1871"/>
            <end position="1885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1886"/>
            <end position="1907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2022"/>
            <end position="2041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2048"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="2318"/>
            <end position="2341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="2580"/>
            <end position="2592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="2621"/>
            <end position="2634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013918441.1"/>
        </source>
    </evidence>
    <sequence length="2749" mass="312462" checksum="3641C25087850EF9" modified="2020-10-07" version="1">MIMKIQKKEKQLPNFKVLTHSPMSDDSITFECKPRSPFDGSPDQEDLSDLLTISDLASVSLAHKDHQNVLSVEVPSEIVQNMITCHNGMQDDGVGGTGVKKKRKKKELGQQELGNEREVKPKRKREPKEPKDPQKPKEPKKPKEPKQEGAKKPRKPREPKAPKESKEKKNILETSSKAKPKKSSMEQGPTPVEKKKKGKRKNDTSLDSLELDQGLLSLSGQNLEESVESVDSQKRRSGRQVKRRKYNEDLDFKVVDDDGETIAVLGAGRASALSASTLAWQAEEPPEDDANIIEKILASRMVQKEIYPGGATYEQEEFYVKYRNFSYLHCKWATMEELEKDPRISQKIKRFRNKQAQMKHIFTEPDEDLFNPDYVEVDRILEVAHTKDSETGEEVTHYLVKWCSLPYEESTWELEEDVDPGKIKEFEFLQIPPELKSLERPASESWQKLEKSRDYKNNNELREYQLEGMNWLLFNWYNRKNCILADEMGLGKTIQSITFLFEIYLMGLRGPFLIIAPLSTITNWEREFRTWTELNAIVYHGSQISRQMIQQYEMVYRDTQGIPLPGLFKFQVVITTFEMILADCPELKKIHWRCVVIDEAHRLKNRNCKLLEGLKLMCLEHKVLLTGTPLQNSVEELFSLLNFLEPQQFPSETAFLEEFGDLKTEEQVKKLQSLLKPMMLRRLKDDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGANQHNMPNLINTMMELRKCCNHPYLINGAEEKILEDFRKTHNSEAPDFQLQAMIQAAGKLVLIDKLLPKLIAGGHKVLIFSQMVRCLDILEDYLIQRRYTYERIDGRVRGNLRQAAIDRFCKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQDINRKGSNNGVQQLSKMEVEDLLRKGAYGALMDEEDEGSKFCEEDIDQILQRRTQTITIQTEGKGSTFSKASFVASGNRTDISLDDPNFWQKWAKIAELDTDARDEKESLVIDRPRVRKQTKHYNSFEEDELMEFSELDSDSDERPTRSRRLNEKTRRYLRAECFRVEKNLLIFGWGRWKDILTHGRFKWHLNEKDMEMICRALLVYCVRHYKGDEKIKSFIWDLITPTKDGQNQALQNHSGLSAPVPRGRKGKKTKSQMLLPEIKNADWLTTCNPEIVLHDDSYKKHLKQHCNKVLLRVRMLYYLKAEVLGEAADKAFEGTPAKDLDVLLPDIDYVEIPVDWWNADADKSLLIGVFKHGYERYNAMRADPALCFLEKVGMPDEKLLCAEQNVTDGVQDAPERGNTDKEESSADRLAGVEKQEEISDTCGPVPEKKDDNVAAQDGSDPDKSSWPVSSALTARLRRLVTIYQRCNRKELPPRPDVIGTCKHGYWLQEEMIRRANEMDPMNNEIQKRWTRREQADFYRTVSSFGVIYEPEKKVFDWTQFRSISRLEKKTDESLEKYFYNFVAMCKNVCRLPHWKDDGPTDPNIYVEPITEERAARTLYRIELLRKIREQVLRCPQLHERLKLCRPSLYLPVWWECGKHDRDLLIGTAKHGLNRTDYYIMNDPQLSFLDAYRNYAQNKRTGLLGEEHLCCHHQANSKLYYSPLYNQAERKQASAEAETENCAKLMISGNDLLQAENASQDGSCENFMSKVRSMISANYDESSLPDSLSCMMYDEKACNSEHSSFARDSPSCTDISSNATKVAEGKTDGDEDLSSCDAETIRESTYLDHLQFGSDQLEGQNLGQELSEKEAKPSESFAQETCNALQHMESQYMSPKAVPFERDGVESVLSIGVYGPSLHNFDIKVQPEKRLMGLLHENSLEEKLMQSQSHSEEEEEEEEEEEEEDHLETVTDIGKDRGEAKCRTQEEQKHNSCILTTLDALMEERNKILVESVSSELAASKCDSEPGMGEDDQAECESLEEHTPGLQEIQTCHHHYHHSARSQASAIQVELLKQHPVCMEGDLWGDDSEEKRDSPVTQSLYESEIKREEEECEHQDLDKRDGISQNQDGFEKFSEDESKSSAFAVPGESDDLHDVRAPTIAQLLQEKTLYSFSEWPKDRVIINRIDNICHAILKGKWPSSSQSFDIPYMMPTPALVSSTCNRSGYTEPEVSESSFSNGMLATQLPKETFLAPTFVKDESKHRQSYEFEMERDAKHRSLDQLAASHSHPSAVLNGWRDAVDLSRVPDGTSASSTPFSMSTNIPKLGTMNALQGSLGMDLSGILQAGLIHPVTGQIVNGSLRRDDAAIRRRRGRRKNVEGIDLILLKERSLQTGLPEDEGQTVTSNSNSDGPVITTSSQQTAPATNVQTDKIVPNKSLLDWLRQQSDYTLEIPGFGTNFIEKPKQRRQRCKDPSKLDINSLTGEERVPVVHKGTGRKLGGAMAPALKELPRWLDANMEYAVAADWSDIVKLSGFLPESKFHRILTEPVLRDVGPRRRGRRPRSELFKAPGIGADASSGMGPLFMNGLIAGMDLVGLQSMRNMQGIPLAGLVGFPAGFAAVPAGEDVKNTLSMLPMMLPGMAAVPQMFGVGGLLNPSIATTCTSTAPTSLASTTKSGTTHSENETEDKQNTPGSKIETVSEDKLSPNSFSDQSEPAITTSSPVAFNPFLIPGMSSGLIYPSMFLSPGIGMALPGLQQTRHSEATNLESQKRKKKKTKGELANVEPETLSLSDKEHANKQNCTEQMPYVSMEHERDVPAEEEEEEELKEIKNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXN6</accession>
    <name>A0A6I9XXN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="25">PARP</shortName>
            <ecNumber evidence="25">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">TNKS2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="25"/>
    <dbReference type="RefSeq" id="XP_013918576.1">
        <property type="nucleotide sequence ID" value="XM_014063101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXN6"/>
    <dbReference type="GeneID" id="106546264"/>
    <dbReference type="KEGG" id="tsr:106546264"/>
    <dbReference type="CTD" id="80351"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09524">
        <property type="entry name" value="SAM_tankyrase1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01438">
        <property type="entry name" value="tankyrase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000012">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000009">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000010">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000011">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000021">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000024">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase tankyrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF143">
        <property type="entry name" value="INVERSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="19 24" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 25" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 25" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0779">Telomere</keyword>
    <keyword evidence="8 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="358"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="394"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="427"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="523"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="556"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="589"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="676"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="709"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="742"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="26">
        <location>
            <begin position="874"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="27">
        <location>
            <begin position="956"/>
            <end position="1161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013918576.1"/>
        </source>
    </evidence>
    <sequence length="1163" mass="127352" checksum="A845E99222CA66CC" modified="2020-10-07" version="1">MLPQPPSSCCVVAGASSEPARELFEACRNGDVERVKRLVRPENVNGRDTAGRKSSPLHFAAGFGRRDVVEYLLQSGANVHARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPSIRNTDGRTALDLADPSAKAVLTGEYKKDELLESARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLASGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHNKSTIDLAPTPQLKERLAYEFKGHSLLQAARESDVNRVKKHLSLEIVNFRHPQTHETPLHCAAASPYPKRKQVCELLLRRGANINEKTKDFLTPLHVASEKAHNDVIEVVVKHEAKVNALDNLGQTSLHRAAHCGHLQTCRLLLSCGCDPSIISLQGFTASQMGNESIQQLLQGNVSFLFVLKIFLNFNKGQLGILTEFQTVKKLCTTHSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLCSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEASQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVTADDVSALLAAAMPLSVLPSCYKPQVINVSQNTGTSADSLSSIPSSPSNLSAASSLDSLSGSFSELSVVNTNSAEGAASLEKKEVLNVDFSINQFVRNLGLEHLIDILEREQITLDVLVEMGHRELKEIGINAYGHRHKIIKGVERLISGQQGLNTYLTLNTSGSGTILIDLSPDDKEFQSVEDEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVTEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPLHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIVKPEIATEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYM7</accession>
    <name>A0A6I9XYM7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lipoma HMGIC fusion partner</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LHFP</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909580.1">
        <property type="nucleotide sequence ID" value="XM_014054105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYM7"/>
    <dbReference type="GeneID" id="106539329"/>
    <dbReference type="KEGG" id="tsr:106539329"/>
    <dbReference type="CTD" id="10186"/>
    <dbReference type="OrthoDB" id="9938692at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019372">
        <property type="entry name" value="LHFPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489:SF12">
        <property type="entry name" value="LHFPL TETRASPAN SUBFAMILY MEMBER 6 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489">
        <property type="entry name" value="LIPOMA HMGIC FUSION PARTNER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10242">
        <property type="entry name" value="L_HMGIC_fpl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013909580.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18029" checksum="0FC65EC94E9D5737" modified="2020-10-07" version="1">MTGKMASSLTYAGVIWALFSFLCAGASCVGFFMPYWLLGSQLEKPVSFGTFRRCSYPVRDESRQMTVMVEQCGRYASFQAIPSVEWQICTVVTGLGSGLLLLVALTALMGCCVYELISRTVGRVAGGIQFLGGLLIGSGCALYPLGWDSEEVRQACGNLSDQFELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y018</accession>
    <name>A0A6I9Y018_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Serine/threonine-protein kinase PINK1, mitochondrial</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Serine/threonine-protein kinase Pink1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">PINK1</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="33">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="26">Upon mitochondrial depolarization, it forms a supercomplex with TOM and TIM23 complexes. PINK1-TOM-TIM23 supercomplex formation requires PINK1 interaction with TOMM20 and TOMM70 and is critical for PINK1 stabilization at the outer mitochondrial membrane, kinase activation and downstream mitophagy. Upon mitochondrial depolarization, interacts with TIMM23; the interaction is required for PINK1 accumulation at the outer mitochondrial membrane, kinase activation by autophosphorylation and PRKN recruitement to mitochondria. Interacts with PRKN. Interacts with FBXO7. Forms a complex with PRKN and PARK7. Interacts with NENF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion outer membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013920087.1">
        <property type="nucleotide sequence ID" value="XM_014064612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y018"/>
    <dbReference type="GeneID" id="106547436"/>
    <dbReference type="KEGG" id="tsr:106547436"/>
    <dbReference type="CTD" id="65018"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090141">
        <property type="term" value="P:positive regulation of mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000418">
        <property type="entry name" value="PTEN induced kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051511">
        <property type="entry name" value="MitoQC_Scaffold_Kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22972">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22972:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PINK1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 33" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="30" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026647021" description="Serine/threonine-protein kinase PINK1, mitochondrial" evidence="30">
        <location>
            <begin position="37"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="76"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00062732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00071830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00074253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_013920087.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="53268" checksum="833A2BBE8CDCEA54" modified="2020-10-07" version="1">MDAKSMRVPLGRLPPAPRRLGLALGLGLALLEPAAAEERRAASACRDIQTIFLPRNKPPKDPLGLLARRGFSLEDYFIGQSIGKGCSGAVYEAAAPAEPPGDGGRGPKRKEGAGGASEAQGAAEAEGWKEGFPLAIKMMWNISAGSTSEGILTTMSQELVPASGRALSGEFGAVARPRGGLCGKKWLKPHPNVIQVIRAFTSQVPLLPGATVDYPDVLPSSLSPSGIGHSRTLFLVMKNYPCTLRQFLSEGRPDPCVGALMILQLLEGVDHLVRQGVAHRDLKSDNILVEFDPAGCPGLVITDFGCCLADQRLGLKLPFPSWYVDRGGNTCLMAPEVATAVPGPGVVIDYSKADVWAVGALAYEILGPGNPFYGQGGPALDSRSYREEELPPLPAPAAPEVKELVGRLLRRNPAKRPSARVAANVLHLSLWGEAAVGRPGLPLPQLVAWLLRQSAAALMTERLGGGRGVVSGLKRGFLAGLDYEELCEAAALLCCWRGAACPAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0X4</accession>
    <name>A0A6I9Y0X4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Coiled-coil domain-containing protein 34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">CCDC34</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916613.1">
        <property type="nucleotide sequence ID" value="XM_014061138.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916614.1">
        <property type="nucleotide sequence ID" value="XM_014061139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0X4"/>
    <dbReference type="GeneID" id="106544761"/>
    <dbReference type="KEGG" id="tsr:106544761"/>
    <dbReference type="CTD" id="91057"/>
    <dbReference type="OrthoDB" id="5981665at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045323">
        <property type="entry name" value="CCDC34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025259">
        <property type="entry name" value="CCDC34/181"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23247:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23247">
        <property type="entry name" value="NY-REN-41 ANTIGEN L15 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13904">
        <property type="entry name" value="CCDC34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coiled-coil" evidence="2">
        <location>
            <begin position="90"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013916614.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="37818" checksum="C93B87652E6BE253" modified="2020-10-07" version="1">MPTSEPPKSRREAVYSSPASSDDEGAGKSSTFSLISDSFHPELSDFHHGGEGETTTITSRGSSIPEKMKHHHSLEQIKDTNKHSLAYNNLSPWEEWFLSKERELRARLQAKAFEEMNQQMEETKVKQERERKKLIAEEKHKEWVQKKNEEERKERQRKLKKEMREKEIKEFEEMQSKEKAKEMYKEWLKKKNTEDLQKKKDEKEKEKLREAELQEKKEKSERMFKEWLQNSKNKPRPASAGYAYVNGKLATYPDGNAYPAPAFCNPIPWKPIPVPPPKEEKYVSVKKNKRPLSSHTHRSSSVLFQKSRSNIYLGSLYRLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2H8</accession>
    <name>A0A6I9Y2H8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tripartite motif-containing protein 12A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106547981</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920741.1">
        <property type="nucleotide sequence ID" value="XM_014065266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2H8"/>
    <dbReference type="GeneID" id="106547981"/>
    <dbReference type="KEGG" id="tsr:106547981"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19762">
        <property type="entry name" value="Bbox2_TRIM7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="92"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="292"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013920741.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="43045" checksum="850693CCE78F8B55" modified="2020-10-07" version="1">MSTEELTRRLKEELICSICLDCFHDPVSIHCGHTFCQACITGYWVFINKKCFHCPKCRKTSRKKILKRNRELGNIAKIVPKLDEKTKEKEKAKEKVCQMHQEPLKLFCKNDQMAICVICDKSKEHKDHTVVPVEQEFQDQLSFLKTNREKLVKIQSVNTARNKGALNTIDAGIQKISLAFKQINQSLKEQKQQLLAQWNSLKAALKEQEIHNATISEEISCLDSFITMAEERWSILDLEFLQEMQNASMRLKQKKVLGFDELLPEMENRLAYQNEVVTSTLRKLEDALISAKREKSNSPIAKKRKSESHSRESRMHSSSSHQPDKRAPVGDNGLTSVKKKEDSNSPIEKKRRLEDQAFDYSPESRKPIQCPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2Y9</accession>
    <name>A0A6I9Y2Y9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LIM domain-binding protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106540580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911230.1">
        <property type="nucleotide sequence ID" value="XM_014055755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2Y9"/>
    <dbReference type="GeneID" id="106540580"/>
    <dbReference type="KEGG" id="tsr:106540580"/>
    <dbReference type="OrthoDB" id="44841at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF9">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zasp-like motif" evidence="3">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013911230.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="23829" checksum="BB8A7D28E22015FC" modified="2020-10-07" version="1">MAISHYLPNKSQRSKRPIPMPTSIPRIDSPMPVIPHQKVSSNSTADADYVERFNPNVLKDSALSTHKPIEVKGPGGKATIIHAQYNTPISMYSQDAIMDAIAGQAQAQGGELAGNLPVKDPHVDSASPVYQAVLKNQNKSEDELDDWSRRSSNLQSKSFRVLAQMTGTEYMQDPDEDALRRSRERFETERNGPRFAKLRNWHHGLSAQILNVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y365</accession>
    <name>A0A6I9Y365_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleolar pre-ribosomal-associated protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013911340.1">
        <property type="nucleotide sequence ID" value="XM_014055865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y365"/>
    <dbReference type="GeneID" id="106540669"/>
    <dbReference type="KEGG" id="tsr:106540669"/>
    <dbReference type="OrthoDB" id="72892at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039844">
        <property type="entry name" value="URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032436">
        <property type="entry name" value="URB1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500:SF0">
        <property type="entry name" value="NUCLEOLAR PRE-RIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500">
        <property type="entry name" value="NUCLEOLAR PRERIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16201">
        <property type="entry name" value="NopRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="URB1 C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911340.1"/>
        </source>
    </evidence>
    <sequence length="590" mass="69029" checksum="FE1B3540E235651C" modified="2020-10-07" version="1">MRAAAYHVLSSFRSHLEAARFREQKQLLYLMDVVQNGIKQPNLRFTFPLTLYIARVAQQILKPEEHMYTKLNRFLLSHQYLDLRKVPGFFHLFYSFDSEHKMEREWVLAIIGEGLRDKNCYQLYDYQRIFHIILSFFHSPLCDEATQYQILEILQNAAYITKAAYELIRDHSLLTWLLSVVEKRFLENKLLIHIISLIDTLWKTNLGNKQTSLSSSSQLKELKENRKFLPIQLINEFLHVLLILLNHIRTNLDPVAFIQFFSVLSSVLEYRTLVIKAFREMGRFVVNEHILSNNDVLILLYKWSIISKDVELQDDLHVVAQQYQIKELLRNTDRNKPRLHSQASIRLAQRRSKIEIEAAAISVWKEVYLNECRTLLRSVFIHWEPVLLETSAKLPTEQKGYRNSDLTCKIAYLVFRWLVTSFLDSNLNIQNALLTFWWLKKCGFGNNIILDYILKDGTLKRAVFKLYSSICNASEVMKLSELSVLNGIMLHLLESQGLAENNFHGAVKKFCLTEVTEVNKAASIFLSSIYIGDIWLGASQPDMFMTHIKLICGATDTERNDKKRLEFEDSMVSLCKDIYSFLSLHRHIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3G6</accession>
    <name>A0A6I9Y3G6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein OZF-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106540817</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911545.1">
        <property type="nucleotide sequence ID" value="XM_014056070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3G6"/>
    <dbReference type="GeneID" id="106540817"/>
    <dbReference type="KEGG" id="tsr:106540817"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000555">
        <property type="entry name" value="Zinc finger protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000250">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000931">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF76">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF46.1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013911545.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="47604" checksum="DE416FEE49B82619" modified="2020-10-07" version="1">QNSHLLRHQRTHTGEKPFECPICGKSFSENSYLVTHQRTHTGEKPFECPICGKSFSENSYLVTHQRTHTGEKPFECPICGKSFSENASLVIHQRIHTGEKPFECPDCGKCFTGNSNLMRHQRTHTGEKPFECPDCGKGFSRNFLLMNHQRTHTGEKPFECSDCGKCFSQNSHLIRHQRTHTGEKPFQCPDCGKCFIRNSHLMGHQRTHTGEKPFECPICGKCFSDNSSLVTHQRTHTGEKPFECSDCGKRFSQNYHLVRHQRIHTGEKPFQCPDCGKCFIRNSHLMRHLRTHTGEKPFECPICGKCFSDNSSLVTHQRTHTGEKPFECSDCGESFSQNSNLMIHQRTHIGEKSLECPDCGKRFSRNSYLVLHQWTHTMAKTFKCPVCGLYFKHKPSLIADKEIHMSQKLKSLEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3S0</accession>
    <name>A0A6I9Y3S0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CCT4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911765.1">
        <property type="nucleotide sequence ID" value="XM_014056290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3S0"/>
    <dbReference type="GeneID" id="106540973"/>
    <dbReference type="KEGG" id="tsr:106540973"/>
    <dbReference type="CTD" id="10575"/>
    <dbReference type="OrthoDB" id="275057at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03338">
        <property type="entry name" value="TCP1_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000010">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012717">
        <property type="entry name" value="Chap_CCT_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02342">
        <property type="entry name" value="chap_CCT_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013911765.1"/>
        </source>
    </evidence>
    <sequence length="543" mass="58511" checksum="8160041E53CD3A0C" modified="2020-10-07" version="1">MPENASTKGAFLNAASGNRNSGGNRRSYQDKDKPAQIRFSNISAGKAVADAVRTSLGPKGMDKMIQDGKGDVTITNDGATILKQMQVLHPAARMLVELSKAQDIEAGDGTTSVVVIAGSLLDACSRLLQKGIHPTIISESFQKALEKGIEVLTSMGQPVELSDRETLLNNATTALNSKVVSQYSSLLSPMSVDAVMKVIDPTTASNVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVANTGVNRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYTQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLGLHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTGDMLGSAEVAEEVNLNGSGRIVKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVVRCLVKKRALIAGGGAPEIELAIRLNEYSRVLSGMESYCVRAYGEALEIIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSISALTLATETVRSILKIDDVVNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3U1</accession>
    <name>A0A6I9Y3U1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 179</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM179 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911820.1">
        <property type="nucleotide sequence ID" value="XM_014056345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3U1"/>
    <dbReference type="GeneID" id="106541024"/>
    <dbReference type="KEGG" id="tsr:106541024"/>
    <dbReference type="CTD" id="388021"/>
    <dbReference type="OrthoDB" id="6423876at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029673">
        <property type="entry name" value="TMEM179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059010">
        <property type="entry name" value="TMEM179-179B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26158">
        <property type="entry name" value="Claudin_TMEM179-179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013911820.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="26607" checksum="9237BE22C7F0DE3E" modified="2020-10-07" version="1">MALSNFLFAQCICYFLAFLFSFIVVVPLSENSNDFHGRCLLFTEGMWLNANLTAERQRFTVQEWGPESACRFSLFAGLLSLLLAAVQAWRTLFFLCKGHEDSFFYAFLNLLISSFVVFVIFIASTIVTVGFNMWCDAITEKGSMPNSCEELQDIDLELNLENSSFYDQFAITQFGLWAAWLTWLGITILAFLKVYHNYRQEDLLDSLIHEKEFLLGRSSSQTSFQEEKSGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y478</accession>
    <name>A0A6I9Y478_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Golgi phosphoprotein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GOLPH3L</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918608.1">
        <property type="nucleotide sequence ID" value="XM_014063133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y478"/>
    <dbReference type="GeneID" id="106546297"/>
    <dbReference type="KEGG" id="tsr:106546297"/>
    <dbReference type="CTD" id="55204"/>
    <dbReference type="OrthoDB" id="2189106at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000005">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF4">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013918608.1"/>
        </source>
    </evidence>
    <sequence length="233" mass="26997" checksum="0C585E123F98DE08" modified="2020-10-07" version="1">MTTLIHRGKRVEVGRHAEKRLEAEEHAVVNQTLGEEDPGDSKDIRLTLMEEILLLGLKDKEGYTSFWNDCISSGLRGGILIELAMRGRVQLEPQSMRKKRLLDRKVLLKSDAPTGDVLLDETLRHLKATEPSETVQTWIELLTGETWNPFKLQYQLRNVRERIAKNLVEKGILTTEKQNFLLFDMTTHPVTNTTEKQRLVKRLQESLLDRWVNDPHRMDRRTSGARQGGFRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8P7</accession>
    <name>A0A6I9Y8P7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HBS1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">HBS1L</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the Pelota-HBS1L complex, also named Dom34-Hbs1 complex, composed of PELO and HBS1L. Interacts with the SKI complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917295.1">
        <property type="nucleotide sequence ID" value="XM_014061820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8P7"/>
    <dbReference type="GeneID" id="106545303"/>
    <dbReference type="KEGG" id="tsr:106545303"/>
    <dbReference type="CTD" id="10767"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16267">
        <property type="entry name" value="HBS1-like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04093">
        <property type="entry name" value="HBS1_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000039">
        <property type="entry name" value="HBS1-like translational GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000035">
        <property type="entry name" value="HBS1-like translational GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037189">
        <property type="entry name" value="HBS1-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109732">
        <property type="entry name" value="HBS1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="15">
        <location>
            <begin position="259"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013917295.1"/>
        </source>
    </evidence>
    <sequence length="685" mass="75639" checksum="AE81DC71B80595E4" modified="2020-10-07" version="1">MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSKRDKTSVSAEPLEEHDYEDSEHSNFISSHQLTGLEKAQLNSCLDHMKEVLGDSVPEKMLVEEVLSKNFDIQKALDSILAQDNKQNGKTKNEDNVIVGKPTKGKVRDSQAIRTDSDIVPKVTKMTISGKKQATGFEVPSANVEENGHNTFIPQKGFSAGDTIGNMGIAEILPKSTFPSQTIQASEDSSVVPTPVKKSAKSKQQLDIKAELEKRQGGKHLLNLVVIGHVDAGKSTLMGHLLYLLGNVNKRTMHKYEQESKKAGKASFAYAWVLDETGEERERGVTMDVGMTKFETKTKIITLMDAPGHKDFIPNMITGAAQADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERFQEIVNKLGQFLKQAGFKESDVAYIPTSGLGGENLVTKSQTSELAKWYTGKCLLEQIDSFKPPQRSVDKPFRLCVSDVFKDQGSGFCVTGKIEAGYVQVGERLLVMPPNETCTVKGIALHEQPVDWAAAGDHVSLTLTGMDIIKINVGFIFCCPKEPIKTCTRFRARVLIFNIEIPITKGFPVLLHFQTVSEPATIRKLLSVLHKSSGEVAKKKPKCLTKGQNALIELQTQRPIALELYKDFKELGRFMLRYGGSTIAAGVVVEIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9Y8</accession>
    <name>A0A6I9Y9Y8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KIF18A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919500.1">
        <property type="nucleotide sequence ID" value="XM_014064025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9Y8"/>
    <dbReference type="GeneID" id="106547007"/>
    <dbReference type="KEGG" id="tsr:106547007"/>
    <dbReference type="CTD" id="81930"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000027">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF73">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="11"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="113"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013919500.1"/>
        </source>
    </evidence>
    <sequence length="858" mass="97229" checksum="E7AAD627F2C11146" modified="2020-10-07" version="1">MSATDEDVCNHIKVVIRVRPENPKERDGNFQKVVQVVDKHLLVFDPKVEEVSFFHGKKLPNRDITKRQNKDLKFVFDAIFDEHSTQLEIFEDTTKTFLDGFLNGYNCTVLAYGATGAGKTHTMLGSPEDPGVMYLTMIQLYNSIDRMKEEKHCSIAVSYLEVYNEQIRDLLVNSGPLAVREDPQKGVLVHGITLHQPKSAEEILQMLDYGNKNRTQHPTDVNASSSRSHAVFQIYLRQQDKTASISQNVRIAKMCLIDLAGSERASATKAKGAVLREGANINRSLLALGNVINALADPKKKNQHIPYRNSKLTRLLKDSLGGNCRTIIIAAISPSSLFFDDTYNTLKYANRAKDIKSSIKSNVVSLDSHISQYVKICEQQKKEILMLKEKLEAYKMGKISVPSNQDTAISTNQQQVEISRLKEILKCVFANREEIRNEVLKLDLQLKENVLKTHYQKNCHEQVEIVCSCSPERLEKATGRLDHRLALLKSRRLHLEKKKEEETKSFEDNTNWLHRLENEMRLLGQDGQIPPELNRDLQCCHLKLEIEDLKTQIEYMKTLASQQEQQYKQTEKILNFSLPILRKQYIALKGMQLANGVIEDDFTELKQLIRREKAVIWADQIVEEESDQNDQLKMSAVMSFPQLVSYRNTPCNTPRNLTVEKVKTSAQRETQYGMPLHKRTRRKLIASPLTIQSLQDSSQSCPNSVVESLSEAFCSITYTPEVCKKPSKTLYTGTVHKATLEEMSHMAQIQEPLKQSSTKDVAYNVTPECHMNSTMLCKNNTDTINCPDAARSKSEAANSRNVLQRLGLPSLLNREPDAVCAKPSYMAMTSAASRKRSLLSSASSSGLTNQHDQVIAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC05</accession>
    <name>A0A6I9YC05_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106548734</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921658.1">
        <property type="nucleotide sequence ID" value="XM_014066183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC05"/>
    <dbReference type="GeneID" id="106548734"/>
    <dbReference type="KEGG" id="tsr:106548734"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921658.1"/>
        </source>
    </evidence>
    <sequence length="96" mass="10737" checksum="A8B0B9CAE6BC4D8A" modified="2020-10-07" version="1">MDMKRLKHSMWQLLTEVPKNQAEEESEGTEKKVNSSSVPKVFSSITKDLLQSLPSMMAKNLSVPLAFACLLHLANEKNLKLQGVEDLSEVLVLPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCB1</accession>
    <name>A0A6I9YCB1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch domain-containing protein 3-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921725.1">
        <property type="nucleotide sequence ID" value="XM_014066250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCB1"/>
    <dbReference type="GeneID" id="106548792"/>
    <dbReference type="KEGG" id="tsr:106548792"/>
    <dbReference type="OrthoDB" id="432528at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF1">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921725.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="41818" checksum="931CF4E1FD38AB93" modified="2020-10-07" version="1">MVDYQNILYIFGGMVDSAFTQKRNPLWMYDTDATKWIACRFPAITGEVQVPVNRKGHSAVVYRGNMYIYGGYIDIKGASQEFWTFHFDTKQWASLSAASSGSSPGPRHGHSAVVYGNGMYLFGGLMGLSEQKDFWMWNFRATNWFSIRRSQGPPRVVGHSALILEDSMLVLGGGISNSNPNSTLWQYHFPSQTWIKLPSSEALSSKAYHQMLPTGFGFQGTADCPLRSLGSLKSKEKGCSRLLTLSKQHTCFFEYLHEEPTYQILNNEGGSDIEMKTFCQSSRDSLGFTPYQTTTSAELNSDPAAELLCEERTSYLNVAKEQEVATMDLTDGGPSLDTLKNECGDIFESSVVMLLVGGKPLSSSSAISFWQIELNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCT9</accession>
    <name>A0A6I9YCT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">V-type proton ATPase subunit S1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106548977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921952.1">
        <property type="nucleotide sequence ID" value="XM_014066477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCT9"/>
    <dbReference type="GeneID" id="106548977"/>
    <dbReference type="KEGG" id="tsr:106548977"/>
    <dbReference type="OrthoDB" id="9985059at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033176">
        <property type="term" value="C:proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030641">
        <property type="term" value="P:regulation of cellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008388">
        <property type="entry name" value="Ac45_acc_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471:SF6">
        <property type="entry name" value="ATPASE H+ TRANSPORTING ACCESSORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026965482" evidence="1">
        <location>
            <begin position="26"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013921952.1"/>
        </source>
    </evidence>
    <sequence length="117" mass="13184" checksum="F723A554C1D6CB7C" modified="2020-10-07" version="1">MARGSAVSWRQLLYAFLVLCKETLSLDHVPMLLWSTDSSLHGLAKTLHEGHIMSMDKLYLLLKDTVLGPEPTNIVLFLQDRLSVEYFTQYNNFSGNESLFHNVQASITIPSGISLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD09</accession>
    <name>A0A6I9YD09_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">GPI transamidase component PIG-S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PIGS</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921944.1">
        <property type="nucleotide sequence ID" value="XM_014066469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD09"/>
    <dbReference type="GeneID" id="106548968"/>
    <dbReference type="KEGG" id="tsr:106548968"/>
    <dbReference type="CTD" id="94005"/>
    <dbReference type="OrthoDB" id="28748at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072:SF13">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013921944.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="21818" checksum="E78C468E4731C1EC" modified="2020-10-07" version="1">MVYNIEPEGSNETSLPRKVEVDMARTMGVFLAQLRLLFGISSGPLPEGALLESPGNEGLTDWELDRLLWSRTVENVATVSTTLTSLAQLLDKISNIVIKDAVASEVYHAVESARQAMAELHLGHLDSAFQASKAAATSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLTKMVWERKQRQKEPTKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDE1</accession>
    <name>A0A6I9YDE1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoglycerate mutase</fullName>
            <ecNumber evidence="10">5.4.2.11</ecNumber>
            <ecNumber evidence="10">5.4.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PGAM1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(2R)-3-phospho-glyceroyl phosphate = (2R)-2,3-bisphosphoglycerate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17765"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="EC" id="5.4.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="10"/>
    <dbReference type="EC" id="5.4.2.4" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013922045.1">
        <property type="nucleotide sequence ID" value="XM_014066570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDE1"/>
    <dbReference type="GeneID" id="106549041"/>
    <dbReference type="KEGG" id="tsr:106549041"/>
    <dbReference type="CTD" id="5223"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004082">
        <property type="term" value="F:bisphosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000007">
        <property type="entry name" value="Phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010713">
        <property type="entry name" value="PRK14115.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="7">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013922045.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="29009" checksum="04B2622C44904743" modified="2020-10-07" version="1">MAAAYRLVLVRHGESAWNLENRFSGWYDADLSPAGQEEARRGGEALRDAKYEFDICFTSVQKRAIRTLWTVLDILDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSFDIPPPPMEPDHPFYSTISKDRRYADLTEDQLPTCESLKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGMSEAAIMELNLPTGIPIVYELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDV4</accession>
    <name>A0A6I9YDV4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BAG family molecular chaperone regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BAG1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922342.1">
        <property type="nucleotide sequence ID" value="XM_014066867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDV4"/>
    <dbReference type="GeneID" id="106549268"/>
    <dbReference type="KEGG" id="tsr:106549268"/>
    <dbReference type="CTD" id="573"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01812">
        <property type="entry name" value="Ubl_BAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017093">
        <property type="entry name" value="BAG-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF16">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037029">
        <property type="entry name" value="BAG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="5">
        <location>
            <begin position="22"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="6">
        <location>
            <begin position="101"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013922342.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="22491" checksum="BC5576D0A4CD6A63" modified="2020-10-07" version="1">MNVVDSGNEKHNLQVDSQEDNQPTVHDLALLTEQVTGVPVSFQKLIYKGKSLKEMEQPLSTLGVKNGCKVMLIGKRNSPEEETELKKLKVLEKSVEQLAKKIDEVNKDLREIQKGFLAKNNQTEALSQLDKRIKGTAEQFMKILEQIDAIILPDNFNDSKQKKKGLVKKVQAFLAQCDTVEGNIGQEMDKLQSKNMVLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE38</accession>
    <name>A0A6I9YE38_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine-rich repeat-containing protein 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LRRC27</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922432.1">
        <property type="nucleotide sequence ID" value="XM_014066957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE38"/>
    <dbReference type="GeneID" id="106549334"/>
    <dbReference type="KEGG" id="tsr:106549334"/>
    <dbReference type="CTD" id="80313"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF35">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="53"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="392"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013922432.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="55536" checksum="B3991EB16566755E" modified="2020-10-07" version="1">MEEKWYYTENNGSGDNRQNTQRRSDSVSSSSRRIHRALEKLVSNSAVSLDLSRKDLQHLTAEMYRLFNLRYLHLEGNSLCVIPEDFFQSLSNLTWLDLRYNKLKELPSGIGCHKQLKTLLLERNPIKRLPVELGNVASLTALNLRHCPLEFPPLEVIQKGLSCILCFLRKNRHQNVEYAESVSPEMPPVEKLNLADIMKSSLDLSDGWPNEEEMIRFKKLRDELIQDERQDYPEDKISSNESLLTLRRTRTKESLSRKSSILSSRDKKNLFPAASSYDFIIQSKMAEECRLAALKEMRGKQALIEQRRKDKEVLQKWRKQSRKIREKKGIYTYSPQKGNTMVSQNAPYATDNIKSNDKLDSWREPSDLDERIETAQNINAIRATDTFRAAKYKEVQNRIKQHTQLMKEKRKKLRGLPKEEMEKANQDLETVEHLEGELTELKKDLHKEYRFTAFTGELLPTPDNLLPTNIFSKMTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YED6</accession>
    <name>A0A6I9YED6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein CMSS1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CMSS1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922526.1">
        <property type="nucleotide sequence ID" value="XM_014067051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YED6"/>
    <dbReference type="GeneID" id="106549405"/>
    <dbReference type="KEGG" id="tsr:106549405"/>
    <dbReference type="CTD" id="84319"/>
    <dbReference type="OrthoDB" id="1929311at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030:SF0">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14617">
        <property type="entry name" value="CMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922526.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="32300" checksum="906F404A60F013C9" modified="2020-10-07" version="1">MADTLDEPWWETPGGTKEPALSDNEEECANTSETVQQTSSSTLVSSKKRKKATECFLNVEKAKSKDEKEIEMKPKRKRKKKISDLLTETVPKPGVPADVQKMLNEHFGAKRSVIELEELKLPDSCFLPANDLTHSFSSYLKEICPKWAKLHKKHTEKSSVVMLVICSSALRCLELIRSMATFKGDGRVMKLFAKHLKIQEQMQMLEKDVIHVGVGTPGRIKALIQQEGLKLTSLKYFILDWNHRDQKLRRLIDIPEIKKETIELLEKELIKLCRNGSVKLGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEL6</accession>
    <name>A0A6I9YEL6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106549482</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922623.1">
        <property type="nucleotide sequence ID" value="XM_014067148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEL6"/>
    <dbReference type="GeneID" id="106549482"/>
    <dbReference type="KEGG" id="tsr:106549482"/>
    <dbReference type="OrthoDB" id="410592at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050900">
        <property type="term" value="P:leukocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF36">
        <property type="entry name" value="INTEGRIN BETA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07965">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="16">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="17">
        <location>
            <begin position="186"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013922623.1"/>
        </source>
    </evidence>
    <sequence length="334" mass="36593" checksum="2A477CE688874619" modified="2020-10-07" version="1">MVPSKRKNPCLNAQENCQSAFSYKHVLALTENASEFESRVGQQRISANLDDAEGGFDAIMQAAICKDQIGWRNVTSLLVFTSDGAFHTAGDGKLGGILMPNDGRCHLDANGVYSKSHLYVGNGQCRCGQCQCQANYTGSACECSLDTNGCDQEGKICNGHGHCACNRCQCDVGWLGSHCADHQMPCEVHRDCAECKAFGTGPLSQNCSNSCSQMVRMLVAPVDERWCQMKGGDGRLLIYLIEKDKTGSILLTVNDRKGPDSTRQPNLMPVLMSGLIVVSIGVLLITLPRAVVEICDRRKFRRLEKERKSALWSQTNNFKFKSATTRVTTKGEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YES6</accession>
    <name>A0A6I9YES6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 420-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106549519</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922683.1">
        <property type="nucleotide sequence ID" value="XM_014067208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YES6"/>
    <dbReference type="GeneID" id="106549519"/>
    <dbReference type="KEGG" id="tsr:106549519"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001485">
        <property type="entry name" value="Krueppel-related zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001997">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000475">
        <property type="entry name" value="zinc finger protein 32 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000873">
        <property type="entry name" value="Zinc finger protein 841"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013922683.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="48319" checksum="A0DEB381631B5B8C" modified="2020-10-07" version="1">MHKGEKPYKCLHCGNSFRQKVHLKSHEMIHTGEKPFQCIECGKSFHCSANLTVHHRIHTGEKPFQCLQCGKRIHSGEKPYQCTECGNCFSYKSHLNNHKRIHTGEKPYKCTECGKCFTRLGTLSIHKRLHTGEKPYKCLECGKNFGDSSQLTSHRRIHSGEKPYQCTECGNCFSYKSHLNNHKRIHTGEKPYKCTECGKCFTRLGTLSIHKRLHTGEKPYECRICGKCFITKNEHILHSRIHTGEKPYQCQECGKCFRDSGELTSHKRIHTGEKPYKCTECGMSFRKSGYLCIHKRIHTGEKPYTCHECGKSFSRNHSLRIHRRLHSGEKPYKCLECGKCYSDSGKLTSHMRIHKGEKPFKCIDCGKSFRDCWNLTVHRRIHTGEKPYECVECGKKSRTWNDFSHHKKTHNMKSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFX6</accession>
    <name>A0A6I9YFX6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphoinositide-3-kinase-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PIK3IP1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922899.1">
        <property type="nucleotide sequence ID" value="XM_014067424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFX6"/>
    <dbReference type="GeneID" id="106549716"/>
    <dbReference type="KEGG" id="tsr:106549716"/>
    <dbReference type="CTD" id="113791"/>
    <dbReference type="OrthoDB" id="9893972at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027080112" evidence="3">
        <location>
            <begin position="21"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013922899.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="18519" checksum="B2CBA6D1500850BB" modified="2020-10-07" version="1">MEAARLLALLLLALQHLCSAAEDATATPSPALPARTPASQEPPEAFQPVRGESAAVQPVVGISQRVKMRPGEKKDLGTLGYVLGIIMVVVLIGIGVGIVLGYLYKRGKDLKERHQQKVNEREMQRITLPLSAFSNPACELVEENVIVVHTNPTPVEEIQGGNTPLMGQAGTPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJT2</accession>
    <name>A0A6I9YJT2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Charged multivesicular body protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Chromatin-modifying protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CHMP7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924025.1">
        <property type="nucleotide sequence ID" value="XM_014068550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJT2"/>
    <dbReference type="GeneID" id="106550615"/>
    <dbReference type="KEGG" id="tsr:106550615"/>
    <dbReference type="CTD" id="91782"/>
    <dbReference type="OrthoDB" id="10250120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057471">
        <property type="entry name" value="CHMP7_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF21">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25239">
        <property type="entry name" value="WHD_CHMP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25880">
        <property type="entry name" value="WHD_CHMP7_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CHMP7 winged helix" evidence="12">
        <location>
            <begin position="154"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924025.1"/>
        </source>
    </evidence>
    <sequence length="452" mass="51104" checksum="CDE5BAC8CB8AD0D8" modified="2020-10-07" version="1">MSSPEEAWVAPAPERDLPLEWETDEERMAFLFSAFKQSREVNATEWDSKMGFWSELVLKWGRRKGGICTCLRELQQAFERRGSVPLSLGTVLRELLRRGKLQRESDFMSSVDSSWISWGVGVFILKPLKWTLSSVLGDSKMPEEEEILIYVEILKEKAEEVYNLYQNSTLSSHPIVGLSELRSLCTSICPDERTFCLVLLQLQKEKRVTVLEQNGNKIMKFARGLHAKVSPMNDVDIGVYQLMQSEQLLSQKVESLTQEAERYKEGARSACKAGKKQQAMRSLKSRKRVERRIAELHSKLDVVQGILDRIYASQTDQMVFNAYQAGLGALKLSMKDVTVEKAESLVDQIQELCDTQDEVAQTLAGVAINGSEADTEELERELDSLLLDSPKRQPYLPSVPPKPLSPASVGLPSFTDAELEAELEKLTYSDGELAQKTDRKASDRQRVLELHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJV3</accession>
    <name>A0A6I9YJV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">GALNTL6</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="RefSeq" id="XP_013924050.1">
        <property type="nucleotide sequence ID" value="XM_014068575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJV3"/>
    <dbReference type="GeneID" id="106550638"/>
    <dbReference type="KEGG" id="tsr:106550638"/>
    <dbReference type="CTD" id="442117"/>
    <dbReference type="OrthoDB" id="6119243at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23477">
        <property type="entry name" value="beta-trefoil_Ricin_GALNTL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000011">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000111">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000107">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase-like 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF129">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE-LIKE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="21">
        <location>
            <begin position="465"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013924050.1"/>
        </source>
    </evidence>
    <sequence length="615" mass="71393" checksum="8C875399F61EE81C" modified="2020-10-07" version="1">MKRKQKRFLQMTLLFLVTLIFLPNIGLWSLYREKHFMKSTVLGEQQVLSRCAKAEKRKQTLPVRFGDGQLYTWTDGLKRKDWHDYERIQKDAMRLGKGEQGKPYPVTEEDSDDSAYRENGFNIFVSNNIALERSLPDIRHPNCKDKVYLEKLPNTSIIIPFHNEGWTSLLRTIHSIINRTPNSLIAEIILVDDFSDREHLKEKLEEYMAHIDKVRIVRTKKREGLIRTRLLGASIAKGEVLTFLDSHCEVNVNWLPPLLNQIALNHKTIVCPMIDVIDHNHFGYEAQAGDAMRGAFDWEMYYKRIPIPPELQRTDPSDPFESPVMAGGLFAINRQWFWDLGGYDPGLEIWGGEQYEISFKVWMCGGGMFDVPCSRVGHIYRKYVPYKVPSGTSLARNLKRVAETWMDEFAEYVYQRRPEYRHLSTGDISAQKELRKHLKCKDFKWFMAAVAWDVPKYFPPVEPPPASWGEIRNVAANLCMDSKHGGTGTELRLDICVKDGSERTWSHEQLFTFGWREDIRPGEPLHTRKFCFDAISHSSPVTLYDCHGMKGNQHWSYKKDKTLFHTVSGSCIDCNPAEKKIFMNRCDPLSETQQWIFEHINMTVIEKIISKETSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMB0</accession>
    <name>A0A6I9YMB0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Endonuclease domain-containing 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ENDOD1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925528.1">
        <property type="nucleotide sequence ID" value="XM_014070053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMB0"/>
    <dbReference type="GeneID" id="106551889"/>
    <dbReference type="KEGG" id="tsr:106551889"/>
    <dbReference type="CTD" id="23052"/>
    <dbReference type="OrthoDB" id="8572289at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039015">
        <property type="entry name" value="ENDOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472:SF8">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN ENDOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026988933" evidence="3">
        <location>
            <begin position="23"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="5">
        <location>
            <begin position="62"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="4">
        <location>
            <begin position="63"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013925528.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="53337" checksum="A5BBF7B83B804326" modified="2020-10-07" version="1">MRAAFPACLLALLAWAASTGEGRVVGDDQPGFAECDAFFYGQAPPVGFPAEQVAKVCQKYRGEPRFATLYSTREKVPLFAAFRYTPGEAAGQEEQEEEQEARWLVEPQIDDPGNKQEEMMPEAEIAEALEHLGRNQALMADYVDSGYERGNLNPSYLHKDDHHVATHTLTNAVPLSPPFQELWHWEVEQLVTHSLAPNCENGKDLHLLSGAIPSSLKVKDKMAVPKSVWLAACCDNGAETWSVGFIKEANAESRLEDLTLEALEKKLPAGAELFKDHCGQDRQDPKKLEAVLRSAKDVQAKKPDKPRVKKPTQSKHTQTAKEESGFLKNLCHFIVTPIWKLVKFVCYLICQLVKGVWCLLRWIVQKILCAVWTFIKGISLALLDIFICLGRVGVSILNGIAKNIYNVLMVTYRILSVPVNLILDIVSFPFYTLGAIPGVLKDIASGIGGLFILVINATTSLVKGLNHVVSFLASKILPTTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMP0</accession>
    <name>A0A6I9YMP0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein FAM187B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106551769</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM187 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925396.1">
        <property type="nucleotide sequence ID" value="XM_014069921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMP0"/>
    <dbReference type="GeneID" id="106551769"/>
    <dbReference type="KEGG" id="tsr:106551769"/>
    <dbReference type="OrthoDB" id="6434091at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039311">
        <property type="entry name" value="FAM187A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32178">
        <property type="entry name" value="FAM187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32178:SF8">
        <property type="entry name" value="PROTEIN FAM187B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026673888" evidence="8">
        <location>
            <begin position="22"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="32"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925396.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="31613" checksum="05728E75A1A8AA28" modified="2020-10-07" version="1">MSSWAPLLAFLLGLLLSIAEGNHYEGHWLGQPCLPGQPCVLAFISNNPVVLRCPRSSYRGHITWQYLDPSWTDEQPLTFLRSGGTSWPLFSHDKLQRLRFKSRLIAGTGSLYISSPSREDSGLYTCRAGDATLAYYEVDFQDAERLHVSHAELGEASLGNVTVPLDQGAQATLFTSWSPWQSCNRCGHPGERKQVGFCYAQLTTMDSEGEMPMPCGMARQKYPTLPQRGPELRIETCQVRCVTSYLAGQEEPHWVPFLVFTNYHPQLKATAHLRCPASSIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMQ2</accession>
    <name>A0A6I9YMQ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox protein PKNOX1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PKNOX1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/MEIS homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925678.1">
        <property type="nucleotide sequence ID" value="XM_014070203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMQ2"/>
    <dbReference type="GeneID" id="106552021"/>
    <dbReference type="KEGG" id="tsr:106552021"/>
    <dbReference type="CTD" id="5316"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000004">
        <property type="entry name" value="Meis2 homeobox isoform 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032453">
        <property type="entry name" value="PKNOX/Meis_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="266"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="268"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="410"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="413"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="428"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925678.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="48746" checksum="68DA1499C02B6962" modified="2020-10-07" version="1">MQVVTELKTEQDPNCLETDAGGLSPSPVESQTPMDADKQAIYRHPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEKEGKPFFCEDPETDNLMVKAIQVLRIHLLELEKVNELCKDFCSRYIACLKTKMNSETLLSGESGSPYSPVQPQSSNFKHDKDSLDLLEQLSSWKESPENPIQSAITGTLSPQGIVVPASALQQGNVTMATVAGGTVYQPVTVVTPQGQVVTQALSPGTIRIQNSQLQLQLNQDLSILHQDDGSSKNKRGVLPKHATNVMRSWLFQHIGHPYPTEDEKKQIAAQTNLTLLQVNNWFINARRRILQPMLDSSCSETPKTKKKTAQNRPVQRFWPDSIASGVAQHQSNELTMSDGAVVTITAPVNMNVDSLQSLSSDGTTLAVQQVMMAGQSEDESVDSTEDDGTDLSTTNISGLALDNSDSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMV1</accession>
    <name>A0A6I9YMV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cullin-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CUL9</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925466.1">
        <property type="nucleotide sequence ID" value="XM_014069991.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551833"/>
    <dbReference type="KEGG" id="tsr:106551833"/>
    <dbReference type="CTD" id="23113"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056405">
        <property type="entry name" value="ARM_CUL7_CUL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021097">
        <property type="entry name" value="CPH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055486">
        <property type="entry name" value="CUL7/CUL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771:SF4">
        <property type="entry name" value="CULLIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771">
        <property type="entry name" value="CULLIN AND GALACTOSE-BINDING DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24742">
        <property type="entry name" value="ARM_CUL7_CUL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11515">
        <property type="entry name" value="Cul7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23168">
        <property type="entry name" value="CUL7_CUL9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="5">
        <location>
            <begin position="1132"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="608"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1427"/>
            <end position="1450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013925466.1"/>
        </source>
    </evidence>
    <sequence length="1590" mass="179194" checksum="BA17B789E5B73840" modified="2020-10-07" version="1">MVGEKRNGNLGSKLQAYAEELIRQRRGHDGHTEYLIRWSILSLDDNVENATQAASTENKMDNILMWMSAEEVYANCPTLLGKRKPEGQRVKEEKSPSTFPSDVTVDEASLHEMKADVRNLVQRANRQMARTSGPESSILNTVHVLSAYASIGSLTGVFKETGALDLLMKMLCNEEKQIRRSAGKMLRALASHDAGSRAYVLLSLSQQDGIEQHMDFDSRYTLLELFAETTSSEEHCMSFEGIHLPQIPGKLLFSLVKRYLCVTSLMDKLNSTTELGGDHQDSSAPSSSFGEKSRVQREFDFSMAMANLISELVRVMGWDRSHERELLSLTNAQPRIIRSIFQPKGSTCTAVQVPLVTSNPSPRKKQGQPFLTLSDFSSRSSYVEYVQENLKPGMTVRMLEEYEEIDAGDEGEFRQSNSGMPPVQVLWQSTGQTYWVHWHMIEIIGSGEQNEENAAHEKVSSLTENLKLPTATQTFYCKPLGGLYCLPYLVDQLYESSGMLSRAEWWELLFFIKKLEQHEQQEIIRFIQENHEEQLSELDEEGLIQLSISVELARKLLPLLSKYCQGCTQNDLQNSHIYLKYTFSKGSSEEGCQNMDITSAKDSGFSEAMASQKPKESISAEMSSPVLSTTEKTDTQLFNKLLHVEGLYSPEIPDEKAKAYFSGKVSGKRNSLEKLADIVDIIQKSSSEVALQVAGLRFIIKILEDEPGQERQIIKVESGPSIREKMVKMLVELLSNQLKEKLLMVTALRLLYVLMAKYDWRILFATEGGVRAVLGCMQEYSPSALVQQAGLAALKVLVGAGNCELLGTSGKSYPLNHSDAQMMREIFASIGSASSKESDNLLCVICIAIEKMLATPGASSAVQNGLLVLNMLINNHKGLAEQLGSCDLRSVLQSCCQTGQSSSEMLARIVLNRLAEHKLPLSQENAESETSFEFKDMDVPTLLKNLHETSFSKDVVLALEHCLCDEGVYPERNVTDILPDHEHFLLLLKTLEQLGAEKTVQMAMMRILNKFLDISQEDALPWHECIEPCLSSMSTHNSEREVVQEFIRFLHRLATTNKDCAVVMCRVGTKEALTKAMDKHNSNLLLVTELRDLVTDCEKYASLYKKMTTSILAGCIQMVLGQIEEHRRSHQPINIPFFDVFLRNLCQGSNVEVKEDKCWERIEVSSNPHRANKLIDKNPKTYWESNGSTGSHYINVYMHRGVVVRQMTLIVASEDSSYMPARIVVMGGENTSSITSELNTVNVLPTANRVVLLENMGRFWPIIQIKIKRCQQGGIDTRVRGIEVLGPKPTFWPIFKEQLCRRTYLFYTTKAHTWCQEILEDRMQLLQLFNRLNSALRHEQVFANRFLPDDEAAQALGKTCWEALITPLVQSITSPDSSGISPMSWLLTQYLENLDAAKNSRSRASIFNSRVRRLSHLLVHVDSSGPELEELKPPVKTNGKNGKNKESSSGVIKPAVNKPCGMTGITQCWKDVVQQQVKCFLESSWQATDFVEQYCRTYQRLRTAMEELFGQQISFMLALCHGFSGGLLQLSFLTAMHVSEQFARYIDRWIQQSWADSGNVETFQCLQQSLEPVLFLADLELANTFERFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNZ4</accession>
    <name>A0A6I9YNZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mas-related G-protein coupled receptor member H-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106551805</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family. Mas subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925435.1">
        <property type="nucleotide sequence ID" value="XM_014069960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNZ4"/>
    <dbReference type="GeneID" id="106551805"/>
    <dbReference type="KEGG" id="tsr:106551805"/>
    <dbReference type="OrthoDB" id="9045751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000193">
        <property type="entry name" value="Mas-related G-protein coupled receptor member E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026234">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334:SF69">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02108">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="59"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925435.1"/>
        </source>
    </evidence>
    <sequence length="348" mass="40002" checksum="B98D5AF2EE5AD985" modified="2020-10-07" version="1">MATILPVVMDAGMINLTYSYNEHHKTNAIDSKDSFYEPDCLKNVIYSLIVLICIPGFIGNGIVIQHLGFKIKRNPFTVYILNLAVAQSGTLIFLFLHGIQYLIDQGFHIEILEGFIWTYCTGQLFLTIISIDRCLALFFPIWYQYRQSICLSTMICCAAWIISLFIYAINYALFLTVWGKAVPPLYHFLTCTSVCAPLIAFSSLTLFVRSYLKSRMKTQEKLPKAILLALFFFLIFSLPLIDICIINVIYNEKRLKFMMYGYLFACLNSSVNPLIYFLVGRKKAYQTMCHGKMSLQRVFEEEEENEKQPIVQNDIPSCCSDKSGNNSYFYISQPGSEKLIQTNLLWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP48</accession>
    <name>A0A6I9YP48_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein-L-isoaspartate O-methyltransferase domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106552237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925936.1">
        <property type="nucleotide sequence ID" value="XM_014070461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP48"/>
    <dbReference type="GeneID" id="106552237"/>
    <dbReference type="KEGG" id="tsr:106552237"/>
    <dbReference type="OrthoDB" id="10257972at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOCS box" evidence="2">
        <location>
            <begin position="117"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013925936.1"/>
        </source>
    </evidence>
    <sequence length="177" mass="20490" checksum="A7BFB26319C1FED4" modified="2020-10-07" version="1">MAQFRTGNGLLTKITRTGPSAWQTKKILAVSFAPLIQPNHLDSGKSRLVHLPPVMVRSLQDLARIAIRGTIKKVIHQETASKNGNGLRNPPRFKRRRIRHRRMETIVFLDKEVFASRISNPLEDNNCEELEEDCREEVEKPMPELKPEPATNILREKILSLPLPDPLKYYLLYYREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPZ9</accession>
    <name>A0A6I9YPZ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 84-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106552574</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926367.1">
        <property type="nucleotide sequence ID" value="XM_014070892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPZ9"/>
    <dbReference type="GeneID" id="106552574"/>
    <dbReference type="KEGG" id="tsr:106552574"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000555">
        <property type="entry name" value="Zinc finger protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000848">
        <property type="entry name" value="Zinc finger protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000180">
        <property type="entry name" value="Zinc finger protein 689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF25">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926367.1"/>
        </source>
    </evidence>
    <sequence length="574" mass="65785" checksum="596040E9050954FC" modified="2020-10-07" version="1">MHTGKKSYHCPDCGKSFITNSQLVIHQRTHTGEKLFECPDCGKGFGQNSNLVEHQRTHTGAKPFKCPDCGKDFSLSSSLVRHQRTHTEEKHFECADCGKHFSQNYELMNHQRTHTGEKPFECPDCGIGFSHNSSLVNHQSIHTGEKPFECSDCGKCFTRNSSLATHKTTHTGEKPFECPDCGKCFSQNSRLVEHQRTHTGEKPYECPDCGKRFSQNYELMNHQRTHTREKPFECPDCGKRFSQNYRLVNHQRIHTGEKPFECPDCGKHFSQNSSMVNHQRTHSGEKPFECPVCGKSFSHNSNLVIHQRTHTGEKPYQCPDCGKRFSQNYCLVNHQRIHTGEKPFECPVCGKSFSHNSNLVNHQRTHTGEKPFECPDCGKCFSQNYRLVNHQRTHTGEKPFECPDCGKCFSQNYQLVNHQRIHTGEKPFECPVCGKSFSHNSNLVNHQRTHTGEKPFECPDCGKCFSQNYQLVNHQRIHTGEKPFECPVCGKSFSHNSNLVNHQRTHTGEKPFECPDCGKCFSQNYQLVNHQRIHTGEKPFECPVCGKSFSNRSYWFRHLCRPAGELQPSDCLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRH3</accession>
    <name>A0A6I9YRH3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear receptor ROR-alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106552717</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926534.1">
        <property type="nucleotide sequence ID" value="XM_014071059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRH3"/>
    <dbReference type="GeneID" id="106552717"/>
    <dbReference type="KEGG" id="tsr:106552717"/>
    <dbReference type="OrthoDB" id="8832025at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06968">
        <property type="entry name" value="NR_DBD_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000003">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044101">
        <property type="entry name" value="NR_DBD_ROR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805:SF3">
        <property type="entry name" value="NUCLEAR RECEPTOR ROR-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="156"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926534.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="38159" checksum="CC3724AA96865DC9" modified="2020-10-07" version="1">MMCLVSAAMKAQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQQRDHPQPPGEAEPLTPSYSITANGLTELHDDLSNYIDGQPPEGSKGDSAVSSFYLDIQPSPDQSGLDINGIKPEPVCDFAPTTGFFPYCSFTNGETSPTVSMAELGSLEVVFIRMCRAFDSQNNTVYFDGKYAGPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLLSADRSWLQEKVKIEKLQQKIQFALQHVLQKNHREDGILTKVRNLRQLSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRU3</accession>
    <name>A0A6I9YRU3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Membrane-associated guanylate kinase inverted 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MAGI3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927022.1">
        <property type="nucleotide sequence ID" value="XM_014071547.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106553113"/>
    <dbReference type="KEGG" id="tsr:106553113"/>
    <dbReference type="CTD" id="260425"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06730">
        <property type="entry name" value="PDZ0_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06731">
        <property type="entry name" value="PDZ1_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06732">
        <property type="entry name" value="PDZ2_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06733">
        <property type="entry name" value="PDZ3_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06734">
        <property type="entry name" value="PDZ4_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000005">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000006">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000012">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000001">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000279">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000002">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000042">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000003">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000131">
        <property type="entry name" value="membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF10">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16663">
        <property type="entry name" value="MAGI_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <keyword evidence="19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PDZ" evidence="17">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="16">
        <location>
            <begin position="118"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="15">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="15">
        <location>
            <begin position="343"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="17">
        <location>
            <begin position="414"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="17">
        <location>
            <begin position="584"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="17">
        <location>
            <begin position="734"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="17">
        <location>
            <begin position="859"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="17">
        <location>
            <begin position="1021"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="664"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1117"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1211"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1286"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1427"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="666"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1128"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1211"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1233"/>
            <end position="1250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013927022.1"/>
        </source>
    </evidence>
    <sequence length="1472" mass="161713" checksum="CFB01B53ED60BE6A" modified="2020-10-07" version="1">MSKTLRRKRHWLSKVQECSVSWGSGSGPDPDLLRGGAERGEFPYFGGRLLTVGDSGGGAGSVLLSGKPPAPGDVLLEVNGTPVSGLTHRDTLAVIRHFREPVRLKTVRPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFIHVDQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNDFDLESQRKRTTSVSKMQKMDTSLPEEEEEEEKVAINGNGGTEYKERHLDSSDWMKTVPSYSQTNSSVDFKNYLSREENLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHINQKTQFENPVLEAKRKKQLGQTDGGLSNPEHSKPFFTRDPSQLIGTLIQTSLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKISSGDVIVDINGTCVLGHTHADVVQMFQLVPVNQYVNMTLCRGYPLPDESEDPVVDIVTATPVLNGQPVTQGDTCILSQDLIPGTVVLDHNSKTGHILVNGRLNGPSTIDFGEERATGSSSGCSQTEMVTIPLIKGPKGFGFAIADSPAGQKVKMILDSQWCHGLQKGDVIKEIYHQNVQNLTHIQVVEVLKQFPVGAEVPLLILRGGPSSPAKTPSIDNKINKQENSGSLETIGEPVPHPMPFPPTSVKSSSPKLDPSEVYLKSKILFEDKPPNTKDLDVFLRKQESGFGFRVLGGDGQDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGITVKGKTHKQVLDLMTSAARNGQVLLTVRRKIYFGADKQPEEITSEVPFISQSGSPRLNRQEIINKQFPEAYDVVLQRKENEGFGFVILTSKNKPPRGVIPHKIGRVIDGSPADQCGKLKVGDRISAVNGQSIIDLSHENIVQLIKDAGNTVTLTVIAEEEHRGPPSGANSARQSPIVQHRVLGPVPASSVSTDRGAIEADGGKEQCNNYKHFSLTEVVGSLGGSTLSQNPGSYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEEGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQSGGNKVLLLLRPGTGLIPDHGGLDSFNPVSSSVTHGEHTSLSSSSHSAYTSEASHLPESEALMKFQMSEKLEKLKNTVPEKKSTLMENHLEIKKTVCPQRQVNKWDHTFIPDNKVMHSTAQFASGNAASKSRSASPQKLSNSPSRDHNQLPFYRSKESETKHLDENYLDHLNKSPKRNESGNLGTIENADIEKVLAKGKRDQSVSPQKYPKRERSREKLNIDFQSKTAANDISSSRLLKTEKVKLGVTKEITKPNFSTKNAKYPSEIQYAKVGEQLLSLSSTNPPGKKILEDETMKSSKQFQKSGLPDDSFISVDKNSRLEADEPLFLNKFTDNLELADNTKDDGMSKSESSSPVKKTIVPGPWKVPSTGHVTKAMGLTNKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRW6</accession>
    <name>A0A6I9YRW6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106553129</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013927047.1">
        <property type="nucleotide sequence ID" value="XM_014071572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRW6"/>
    <dbReference type="GeneID" id="106553129"/>
    <dbReference type="KEGG" id="tsr:106553129"/>
    <dbReference type="OrthoDB" id="77911at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44927">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44927:SF1">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="3">
        <location>
            <begin position="169"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013927047.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="38340" checksum="2EE69A68E16D216D" modified="2020-10-07" version="1">MDQTASGTGNEFFQYTAPKQPKKNLPAAGVGPPTQKPPAPAGAPSVFVATAVHAYRFTNGTYVKQGKYGAAVVGSHATQEYRILLYINQQQQITSARIHPGFVFTIQPNNYGTFYDDQRQNWSLMFESEKAAVEFGKQLCLAKYNSSLSPDSVVCQDLLLGDGPAVEVGDALEVSFTGWLLQNSALGQVFDSNVNKEKLLRLKLGSGKVIKGWEEGMLGLKKGSKRFLIIPPALAYGSQGVANRVPPDSTLAFEVEVKRVRLAKEAASNGQNVASRDTLAPSPGPNIEHLSADPAGLPPSTLPPKPGEPALRAKSNSLSEQLAVSLWFTRIGFHFSRFLISGFDLNQGWDSHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSQ9</accession>
    <name>A0A6I9YSQ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PIN2/TERF1-interacting telomerase inhibitor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PINX1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927166.1">
        <property type="nucleotide sequence ID" value="XM_014071691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSQ9"/>
    <dbReference type="GeneID" id="106553227"/>
    <dbReference type="KEGG" id="tsr:106553227"/>
    <dbReference type="CTD" id="54984"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF27">
        <property type="entry name" value="PIN2_TERF1-INTERACTING TELOMERASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="26"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927166.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="37592" checksum="455340FA87697161" modified="2020-10-07" version="1">MAMLAEPRHKLKWSVDPRNSTWSKDESKFGQKMLEKMGWSKGKGLGAQEQGSTEHIKVQVKNNTLGLGASVSQEDNWIAHQDDFNQLLAELNNNYTQDDPESTYKNRKKTFNLEEKSKISKKRVHYVKFAKGKDLSSRSEDDLSCIFGKRKVKKMQDDAATTDFLAEDSKKVIVPPDQIDGSNTVTSTLTVQEYFAKRMKKLKKTQHCTKVEPGNSPPAMVEKLECLESQTKKKKKRHKHAQDEAGEPTIICTGILDSSRDEVDTQKKKKRKKSKRLDTSNDLIYNESQIGNQKARQCSFETSKEENNDLGEQKSKKRKKSKKSRLECN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSW9</accession>
    <name>A0A6I9YSW9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CREB-regulated transcription coactivator 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106552861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926730.1">
        <property type="nucleotide sequence ID" value="XM_014071255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSW9"/>
    <dbReference type="GeneID" id="106552861"/>
    <dbReference type="KEGG" id="tsr:106552861"/>
    <dbReference type="OrthoDB" id="8947034at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008140">
        <property type="term" value="F:cAMP response element binding protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024786">
        <property type="entry name" value="TORC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024784">
        <property type="entry name" value="TORC_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589:SF6">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12885">
        <property type="entry name" value="TORC_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transducer of regulated CREB activity middle" evidence="2">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926730.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="27299" checksum="5DBC8928DC41D80E" modified="2020-10-07" version="1">MVEPPEFPLLSEAVVNRASPLFTPSLATAVVLTESQPISRLQLQAQKLRLAHSRGPYYGGSLPNVNQIGSGAAEFQGPLHSPLDSARGTRHHGLVERVQRDPRRMVSPLRRYVRQIDSSPYSPAYLSPPAEPSWRRTMPWGNFPVEKERLFRLPASLNRTNSDSALHTSVMNPNPPNAYLGPSQGGPPPNRKRGEWLMGGEVGVITRPGTCVSVCVSRERPGWAGEKVHTLARIWGFLPRRFEMMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTI7</accession>
    <name>A0A6I9YTI7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Actin-binding LIM protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106553336</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927284.1">
        <property type="nucleotide sequence ID" value="XM_014071809.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106553336"/>
    <dbReference type="KEGG" id="tsr:106553336"/>
    <dbReference type="OrthoDB" id="1746725at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.950.10:FF:000001">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032402">
        <property type="entry name" value="AbLIM_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF18">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16182">
        <property type="entry name" value="AbLIM_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="6">
        <location>
            <begin position="390"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013927284.1"/>
        </source>
    </evidence>
    <sequence length="458" mass="52032" checksum="780A3975C8A6DBFB" modified="2020-10-07" version="1">MRSEWEESQGDGGNISLGDWPEKTKERLEGAVLLKEESSTLHPNAGTCFKRIAEVTLTHTVRVFCYNKREKPILCIWLSGTPRCYEPLAPLPPSASAVPVWQDRTIASAKLRLLEYSAFMEVQRDPETISDGXHDESSPRTLSPTPSAEGYQDMRERMMHRSTSQGSIGSPVYSRHSYTPTMSRSPQHFHRPDQGINIYRKPPIYKQHDAAALAAQNKSFDDIIKSSKFPAAHAPAPNEIPKIETEYWPGPPSLAAVVGFDMRRRSTGRDEDLEELQRRRQLQEEQLMKLNSGLGQLILKEEMEKEKSLLASRCYDSPANSCLSPSHAAASKTSSLPGYGRNGLHRPMSTDFGQYNSYGDVSNATRDYQFNPDGLVTGMRMDRGVSMPNMLEPKIFPYEVLMVTNRGRNKILREVDRTRLERHLAPEAFQEIFGMTIQEFDKLPLWRRNDMKRKAKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTN9</accession>
    <name>A0A6I9YTN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">EMC1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927677.1">
        <property type="nucleotide sequence ID" value="XM_014072202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTN9"/>
    <dbReference type="GeneID" id="106553655"/>
    <dbReference type="KEGG" id="tsr:106553655"/>
    <dbReference type="CTD" id="23065"/>
    <dbReference type="OrthoDB" id="28092at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="11">
        <location>
            <begin position="11"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927677.1"/>
        </source>
    </evidence>
    <sequence length="141" mass="15980" checksum="1E9DA5E4D9F2F501" modified="2020-10-07" version="1">MRGSTELAKAVGLPSGAILSLPKALLDPRRPEVPTEQTREENLIPYSPDVQIHAERFINYNQTISRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRVWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YW78</accession>
    <name>A0A6I9YW78_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Semaphorin-4C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106554183</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928269.1">
        <property type="nucleotide sequence ID" value="XM_014072794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YW78"/>
    <dbReference type="GeneID" id="106554183"/>
    <dbReference type="KEGG" id="tsr:106554183"/>
    <dbReference type="OrthoDB" id="9934005at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF16">
        <property type="entry name" value="SEMAPHORIN-4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sema" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928269.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12191" checksum="86BB4B4B20B99152" modified="2020-10-07" version="1">LHSSVNSVTELSPGLFVCAENGWLHKAVALPSGVHLIEELQVFEEAQPIKSLVLSVPKRVLFIGSDTKVIQVPVANCSKYRTCSDCILAKDPYCAWTWNGSRCVRIDAYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWP8</accession>
    <name>A0A6I9YWP8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Homeobox protein six1a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106554866</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIX/Sine oculis homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929078.1">
        <property type="nucleotide sequence ID" value="XM_014073603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWP8"/>
    <dbReference type="GeneID" id="106554866"/>
    <dbReference type="KEGG" id="tsr:106554866"/>
    <dbReference type="OrthoDB" id="3501850at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000063">
        <property type="entry name" value="SIX homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031701">
        <property type="entry name" value="SIX1_SD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390:SF61">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16878">
        <property type="entry name" value="SIX1_SD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="105"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="107"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013929078.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="28784" checksum="06FD95EE814EC82B" modified="2020-10-07" version="1">CEVLQQGGNIERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILEGYQFSPHNHPKLQQLWLKAHYAEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRSVLREWYIHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAETKERENGEGCVLKEGHGLPLDRHLIPSSEDESSLIDSPSLLYPALQLSVPEGSSIHNAQPVDYLQNQPLHNGLLGSLTSTLVNLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX25</accession>
    <name>A0A6I9YX25_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Caspase recruitment domain-containing protein 14-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106554980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929208.1">
        <property type="nucleotide sequence ID" value="XM_014073733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX25"/>
    <dbReference type="GeneID" id="106554980"/>
    <dbReference type="KEGG" id="tsr:106554980"/>
    <dbReference type="OrthoDB" id="8795751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050700">
        <property type="term" value="F:CARD domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000003">
        <property type="entry name" value="Caspase recruitment domain family, member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14559">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14559:SF1">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="5">
        <location>
            <begin position="40"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="150"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="310"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929208.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="51265" checksum="B844BE669952A12F" modified="2020-10-07" version="1">MLSQHVLEGNSVDQQEQVSIMGETGVDDPSLVDQELRDMDEDRLWEMVEGHRYRIVRGVSPSRLTPYLRQAKVLGQLDEEEILHSPQFTNRAMRVGHMLDLLKSRGKNGALAFLESLKLHNPDIYVLITGLEPSIDHNNFSDLIESSQLTECLAKAVSSLQEELNQEKRQKVSLLQHCRRLKENNARLEAEAESLRGIETECNRMKRELSAHFHEALKLKDELYGLSLRYSSALQEKDLAITRCRSMQEELYLMKQELQRAQVSPCHQRKSPSRTSGDTQMQTDELLKLTEENQHLKSLLELETFSVVQKDILEQNLDDALEGKQELLNRLHSLREQAALAENQRKQCWEEKENTLIECQKMKVDCEIYKEQISALQAQIADLQKERNQAYNARDSAQLEISQSLLEKDSLRRKVFELTNEICDVRKKASMGDSVLTVSYDCGWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY79</accession>
    <name>A0A6I9YY79_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transient receptor potential cation channel subfamily A member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928891.1">
        <property type="nucleotide sequence ID" value="XM_014073416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY79"/>
    <dbReference type="GeneID" id="106554692"/>
    <dbReference type="KEGG" id="tsr:106554692"/>
    <dbReference type="OrthoDB" id="1661883at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050663">
        <property type="entry name" value="Ankyrin-SOCS_Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193:SF121">
        <property type="entry name" value="ADA2A-CONTAINING COMPLEX COMPONENT 3, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928891.1"/>
        </source>
    </evidence>
    <sequence length="78" mass="8695" checksum="6CFE111A9A47A197" modified="2020-10-07" version="1">MIKMCIEYGAQIDLKQNDNCTALHFAATQGATEILKLMMSSYTGEESIINALDGNKETLLHRAALFDHCELAEYLISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZM4</accession>
    <name>A0A6I9YZM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Zinc finger E-box-binding homeobox 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Smad-interacting protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Zinc finger homeobox protein 1b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">ZEB2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin. Represses expression of MEOX2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 24">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929060.1">
        <property type="nucleotide sequence ID" value="XM_014073585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZM4"/>
    <dbReference type="GeneID" id="106554848"/>
    <dbReference type="KEGG" id="tsr:106554848"/>
    <dbReference type="CTD" id="9839"/>
    <dbReference type="OrthoDB" id="7491548at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048023">
        <property type="term" value="P:positive regulation of melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000013">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000082">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000105">
        <property type="entry name" value="Zinc finger E-box binding homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000117">
        <property type="entry name" value="Zinc finger E-box-binding homeobox 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051574">
        <property type="entry name" value="ZnF_E-box_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391">
        <property type="entry name" value="HISTONE H4 TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391:SF11">
        <property type="entry name" value="ZINC FINGER E-BOX-BINDING HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15 24 29" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16 24 29" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18 24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="26">
        <location>
            <begin position="698"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="1045"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="1073"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="1101"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="24">
        <location>
            <begin position="700"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="99"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="801"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="879"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1162"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="827"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="886"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1173"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="1202"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1215"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1233"/>
            <end position="1252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00058733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00082894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013929060.1"/>
        </source>
    </evidence>
    <sequence length="1261" mass="141592" checksum="AD46BD269F145110" modified="2020-10-07" version="1">MGELKGGKRSRDPMQEMVGVTLKSLRVAESESRVHANEPSDPLLSMKQQIMADGPRCKRRKQANPRRKNVLNYENVLETGSETDEEDKLHIAEDDGIIGTLDQEPSPASVPNHESSPHVSQALLPREEEEDEMRESGVDHAWHNHDILQASVDGSEEMKEEYHTMGPEAAIQTTGNNGTVKNANCTSDFEEYFAKRKLEEGDGHSVSIAEYLQRSDTAIIYPEAPEELSRLGTPEANGQEENDLPPGTPDAFAQLLTCPYCDRGYKRLTSLKEHIKYRHEKNEENFPCPLCNYTFAYRTQLERHMVTHKPGTDQHQMLTQGAGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLISVNGRMRNNIKTGSSPNSVSSSPTNSAITQLRNKLENGKPLSMSEQSGLLKIKTEPLDFNEYKVLMTTHGFAGANPFMNGRLGATSPIGVHTSTQSPMQHLGVGMEAQLLGFPAIGSNLSEVQKVLQIVDNTVSRQKMDCSPEEISKLKGYHMKEPCSQPEEQGITSPGIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQLNNLKKEKLRTLIDLVTEDKMIENHNIPTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENMAPSKPGMFVDKQALLLSSVLSEKGMPSPINPYKDHMSVLKAYFAMNMEPTSDELLKISIAVGLPQEFVKEWFEQRKVYQHTISRSPPLERGNAKVVLAATNNTPIKDSLSARSPIKPVDSITSPSIAELHNSVTNCDTPLRLTKPTHFTNIKPVEKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKLMKEPKNIIATKNKTKSNSVNVDHNSVSSSSETSDEPLNLTFIKKEFSNSNSLDKSTNPLFAMNPFSAKPLYTTLPPQSAFPPATFMPPVQTSIPGLRPYPGIDQMSFLPHMAYTYPTGAATFADMQQRRKYQRKQGFQGELLDGTPDYMSGLDDMTDSDSCLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREAREKGHLETTELLMNRAYLQSITPQGYSDSEERESMPRDGESEREHEKEGEDGFEKLGRQDGDEEFEEEEEEESENKSMDTDPDTIRDEEEAGDHSMDDSSEDGKMETKSDHEEDTMEDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2U2</accession>
    <name>A0A6I9Z2U2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">LOC106555905</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport. Negatively regulated by ARL6IP5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>K(+)(in) + L-cysteine(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-cysteine(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:82559"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>K(+)(in) + L-glutamate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-glutamate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Apical cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Membrane</location>
            <topology evidence="28">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Recycling endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="28">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930311.1">
        <property type="nucleotide sequence ID" value="XM_014074836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2U2"/>
    <dbReference type="GeneID" id="106555905"/>
    <dbReference type="KEGG" id="tsr:106555905"/>
    <dbReference type="OrthoDB" id="5877963at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033229">
        <property type="term" value="F:cysteine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF109">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0915">Sodium</keyword>
    <keyword evidence="12 28" id="KW-0769">Symport</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="9 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 28" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013930311.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="20322" checksum="5FC742F7D282DC1B" modified="2020-10-07" version="1">MGKETPGKKEGCDCKRFLKNNWLLLSTILAVVLGIGLGLAVREYGNLSNLDKFYFAFPGEVLMRMLKLIILPLIISSMITGVAALDSSVSGKIGLRAVVYYFFTTALAVILGIVLVVTIKPGVSQKADEIGRMGSTPEVSTVDAMLDLIRNMFPENLVQACFQQVDSIFVFINRRSVKFWHLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3T1</accession>
    <name>A0A6I9Z3T1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calcineurin-binding protein cabin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CABIN1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930520.1">
        <property type="nucleotide sequence ID" value="XM_014075045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106556081"/>
    <dbReference type="KEGG" id="tsr:106556081"/>
    <dbReference type="CTD" id="23523"/>
    <dbReference type="OrthoDB" id="77564at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000076">
        <property type="entry name" value="calcineurin-binding protein cabin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="4"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="273"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1224"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1317"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1234"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930520.1"/>
        </source>
    </evidence>
    <sequence length="1546" mass="174842" checksum="6854521EAF34F00F" modified="2020-10-07" version="1">MLKYSTYKNLAQLAAQRDNIETAMEFYLEAVLLDSTDVNLWYKIGHVALRLTRLPLARHAFEEGLACNPDHWPCLDNLITVLYTLSDYTTCLYFICKALEKDNSYSKGLVIKEKIFEEQPCLRKDSLRMFLKGDLPVCDVPVSASETQTILEEALELRRKRQALMARKNEPDLKLAQPISLFTWKSLGESLMATYLHLTTCEPPRPSLGKQIDLLEYQDLDQHLESQANVAPVSVLHPSPVSSNPLVPLTEPLLTYTPLTPNFPSLTVLESVNSAGDASGGDKSKKGLKRKKIMEESGETAKRRSARVRNTKCKKEEKVDFQELLVKFLPSRLRKLDPEEEGPFNHYEIQSTTKDENSQPLGSHRMSLDLTVCMESEKQDVHKFLLANLNNGGILELMMRYLKVISQKFLVKWPPGLPEVVLNIYNNWRKHSSSLPNPLLRDCHNQHIREMMLMSLSCMELQLDQWLLAKGKSSMVSPRNGPAASLANGKFGPDFPGTHFLGDLLQLSFASSQRDLFEKSWLQFAVRVYWLKARFLALQGEMEQALENYDVCTQMLQHSAALKADSDSDKRMVIRLPNLHVDSMVSLEEIDKNLKSLERCQSLEELQRLYEAGDYRAVVQLLRPTLCLNGDSRTEHLELAISIPERPAQLLLLQDSLLRLKDYQQCLECSEVALNEAFQQMINTTTASAKEEWVATVTQLLQGISHSLSANSSSLSEVSALPNLTRFSHNLIQIIDSSMVVQEEPKEPFVSSVLPWIILHQIIQHEEENIQPLYCQHEKLQNPQEGDTPMLPPSLMLLNTAHEYLGRRSWCCNSDGALLKFYVRVLQKELLASTTEDTHPYKEELETALEQCFYCLYGFPSKKSKARYLEEHSTQQVELMWEDSLFMFEYFKPKTLPEFDSYKTSTVSADLANLLKKMATIVPQTDKPMLRLEEVSSYIEGTLSKVPSLPEGADFSPPVVTELYYLLADYHFKNKEQSKAIKFYMHDICICPNRFDSWAGMALARASRIQDKLNSNELKSDSLIWKQSTPVLSCFKRALEIDGSNLSLWIEYGTISYALHSFASRQLKQWKPELPSEVVQQMEERRNGMLETAKHCFTSASRCEGDGDEEEWLIHYMLGKIAEKQKQPPGVYLFHYKQAGHFLHEEAARYPKKIHYHNPPELAMEALEVYFRLHASILKLVGKPDSGADAELLVTFMKEAAEGSFARGEEKNTPKLSEKEKLCMADEDSHSSAGTLPGPGTSLLSSSGPGLTSPPYTATPVDHDYVKCKKSHQQATPDAAFISQQDPASLGLEDRSQDSMAVGLSDSNSTQDTFTELASLPDSNKKTFPEGRPLASCLDATAPGKEILVPTEERGKPEDPLGSPDACRAAEQKPSPQGPCKFSAPPLSQGDPNRPTELLCTSQFAQGLPTDLEEQRTFLTEHCIAAFHLCLSRFPQHYKSLYRLACLYACSPTHKNLPWARDVLLGSSFPWQQLRHMPAQGLFCERNKTNFFNGIWRIPVDEIDRPGSFASHMNRSIVLLLNVLSQLKDYPTLLKISSMLQRTPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z484</accession>
    <name>A0A6I9Z484_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Partitioning defective 6 homolog alpha-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106556574</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAR6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931034.1">
        <property type="nucleotide sequence ID" value="XM_014075559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z484"/>
    <dbReference type="GeneID" id="106556574"/>
    <dbReference type="KEGG" id="tsr:106556574"/>
    <dbReference type="OrthoDB" id="5868434at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060341">
        <property type="term" value="P:regulation of cellular localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06403">
        <property type="entry name" value="PB1_Par6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06718">
        <property type="entry name" value="PDZ_Par6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000031">
        <property type="entry name" value="Partitioning defective 6 homolog alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000030">
        <property type="entry name" value="Partitioning defective 6 homolog beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051741">
        <property type="entry name" value="PAR6_homolog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034868">
        <property type="entry name" value="PB1_Par6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14102">
        <property type="entry name" value="PAR-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14102:SF9">
        <property type="entry name" value="PARTITIONING DEFECTIVE 6 HOMOLOG ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="PB1" evidence="14">
        <location>
            <begin position="15"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="153"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="254"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="329"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013931034.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="39013" checksum="42A67F488AB2FBD5" modified="2020-10-07" version="1">MAKNHRTPARSSEPVIEVKSKFEAEFRRFAMKRSSVGGFQEFYQLIQRVHQIPCSEVLLGYTDIHGDLLPINNDDNYHKALSSANPLLRIIIQKRAEANASVFASNSLQRKKKGLLRPTHQRAKPHLLIGMPQDFRQISSIIDVDILPETHRRVRLHKHGSNKPLGFYIRDGVSVRVAHHGVEKVPGIFISRLVKGGLAESTGLLAVNDEILEVNGIDVAGKSLDQVTDMMVANSHNLIITVKPANQRNNVIRSSKASGSSGMSTDSTPSQQTPSPASQYLSNCSTAEGESDEEGDLVIESDLPASCPNGGLLDSLQHSLSLYSSQGSLPSLNSHSGSGSPMRGSRTGSLQEDGTIITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4H6</accession>
    <name>A0A6I9Z4H6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch-like protein 33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106556677</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931129.1">
        <property type="nucleotide sequence ID" value="XM_014075654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4H6"/>
    <dbReference type="GeneID" id="106556677"/>
    <dbReference type="KEGG" id="tsr:106556677"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF14">
        <property type="entry name" value="KELCH-LIKE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21536">
        <property type="entry name" value="BTB_KLHL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BACK" evidence="3">
        <location>
            <begin position="76"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931129.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="34335" checksum="0BD4254817C9FD14" modified="2020-10-07" version="1">MFTSGMKEAQQAKVPLCTQFTAADLGLIISFAYSGVLRGGWDYIFEAAQAALQYQVSGILDLCLDLFRYKLTPETCLDVLSFARAYGLPDIENTAEKFILNSFVRVTKTAKFLDLPINQLVDFLSSDSLYILNELEAFQGAVRWLTSDWAGRMNCAEKVLHCIRFPLMSNRELKQVRKEEMMADPGRLYNLMVQSLASIPPGPSKMEQLPCRVRYPEKVIVISGGDTLTKNMATRSPCQDFWFTHRFLNGIGLVKQVEWRRLGYLPEKPRFRHAVVVKDNKMYLFGGKHYYGVRDTMCSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5C5</accession>
    <name>A0A6I9Z5C5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Caprin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CAPRIN1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the caprin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931409.1">
        <property type="nucleotide sequence ID" value="XM_014075934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5C5"/>
    <dbReference type="GeneID" id="106556892"/>
    <dbReference type="KEGG" id="tsr:106556892"/>
    <dbReference type="CTD" id="4076"/>
    <dbReference type="OrthoDB" id="10062814at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028816">
        <property type="entry name" value="Caprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022070">
        <property type="entry name" value="Caprin-1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041637">
        <property type="entry name" value="Caprin-1_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22922:SF3">
        <property type="entry name" value="CAPRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22922">
        <property type="entry name" value="GPI-ANCHORED PROTEIN P137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12287">
        <property type="entry name" value="Caprin-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18293">
        <property type="entry name" value="Caprin-1_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Caprin-1 dimerization" evidence="10">
        <location>
            <begin position="124"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoplasmic activation/proliferation-associated protein-1 C term" evidence="9">
        <location>
            <begin position="350"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="411"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="512"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="455"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="525"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="568"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="633"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="667"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013931409.1"/>
        </source>
    </evidence>
    <sequence length="700" mass="77581" checksum="35409B6E2F0ED378" modified="2020-10-07" version="1">MPSATSSAMASNGSKAGSEATVAAAAASSQAAVSGGSTSSVQTEAMKQILGVIDKKLRNLEKKKGKLDDYQDRMNRGERLNQDQLDAVSKHQEVSNNLEFAKELQRCFMALSQDIQKTIKKTARREQLMREETEQKRLKTVLELQYILDKLGDDEVRSDLKQGTNGVSILTETELSTLDEFYKLVEPERDMNIRLNEQYEQASIHLWDLLEGKEKPVCGTTYKALKEIVERVLQTSYFDSTHNHQNGLCEEEETASAPAVEDSVTETEPEPTEEYTEPSEVESTEYVNRQFMAEAQFSNNEKEQVDEWTVETVEVVNSLQQPQATSPPVPDAHTLTAVAQADPLIRRQRVQDLMAQMQGPYNFMQDSMLEFENQALDPAIVSAQPMNPQTLQIPQMVCPPVHAESRLAQTTQVPVQPEATQVPLVSSTSEGYTASQPTYQPSHSTEQRPQKESIDQIQASITLSTDQTPTSSPLPVASQPQVFQAASSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQTGYSQTFSNQPHPVEQSELQQEQQLQTVVGTYHGSPDQSHQVAGNHQQPPQQNTGFPRNGQPYYNSRGVSRGGSRGTRGLMNGYRGPANGFRGGYDGYRPSFSNTPNSGYTQAQFNAPRDYPNYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGMAQMNAQQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5P9</accession>
    <name>A0A6I9Z5P9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ZDHHC18</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013931987.1">
        <property type="nucleotide sequence ID" value="XM_014076512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5P9"/>
    <dbReference type="GeneID" id="106557297"/>
    <dbReference type="KEGG" id="tsr:106557297"/>
    <dbReference type="CTD" id="84243"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF257">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="164"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931987.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="46076" checksum="F231E21C09B415C8" modified="2020-10-07" version="1">MKDCEYRQIPPGTPAAPAASGPPTSVAASTAPTTLAPRPRRKWEVFPGRNRFYCGGRLMLARHSSVFLLTVGLIVVTSGLFFAFDCPFLAHNLTIAIPIIAGILFFFVISCLLQTSFRDPGILPRATPSEAADLEKWIDNLGTSTYRPPARTMEVVINKYMVKLKYCYTCKMFRPPRTSHCSVCDNCVERFDHHCPWVGNCVGKRNYRFFYAFILSLSFLTAFIFACVITHLALRSQGNDFLTVLKTTPASLLELVICFFSVWSIFGLSGFHTYLIASNLTTNEDLKGAWSNKRGSEFANPYSHKSVLTNCCAVLCGPFYPSLIDRRGFVQADAGTPSTPKAEIPSFGTKSDASMYVQGTKDLLRTLCASWQNSRRTQETPVKYLKIQPLPPPVPLNPPGLSNFSIAASLSLSQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5X4</accession>
    <name>A0A6I9Z5X4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytosolic carboxypeptidase 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_013931336.1">
        <property type="nucleotide sequence ID" value="XM_014075861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5X4"/>
    <dbReference type="GeneID" id="106556841"/>
    <dbReference type="KEGG" id="tsr:106556841"/>
    <dbReference type="OrthoDB" id="10253041at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF5">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931336.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="32573" checksum="C69A04EC0D647291" modified="2020-10-07" version="1">MSGVLGFLEFPGTNRRVQYTISKGGSEALLQALVNAARTSVPDYDILLPLLRLLVRIGCKVAVGTMLGINGAMELLFQVITPYTKKHTITTRYEYDLILSPDINSAQYHQWFYFEVSAMKAAIPYHFNVINCDKPNSQFNYGMQPVMYSVKEALQGRPHWIRVGYDISYYKNHYRNCAPAAAGGPRGKSCYTLTFSLVFPHQEDVCYLAYHYPYTYSTMMSHLDILEQTRNTKKVFFKRQTLCHTLAGNACPLLTITAMPESESRNDLEQFRKSVLSFARPPLDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6G7</accession>
    <name>A0A6I9Z6G7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein FAM65A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106556970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RIPOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931516.1">
        <property type="nucleotide sequence ID" value="XM_014076041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6G7"/>
    <dbReference type="GeneID" id="106556970"/>
    <dbReference type="KEGG" id="tsr:106556970"/>
    <dbReference type="OrthoDB" id="9999654at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031780">
        <property type="entry name" value="FAM65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026136">
        <property type="entry name" value="RIPOR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15829">
        <property type="entry name" value="PROTEIN KINASE PKN/PRK1, EFFECTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15829:SF1">
        <property type="entry name" value="RHO FAMILY-INTERACTING CELL POLARIZATION REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15903">
        <property type="entry name" value="PL48"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM65 N-terminal" evidence="3">
        <location>
            <begin position="83"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="FAM65 N-terminal" evidence="3">
        <location>
            <begin position="243"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="FAM65 N-terminal" evidence="3">
        <location>
            <begin position="357"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="460"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="514"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="592"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931516.1"/>
        </source>
    </evidence>
    <sequence length="801" mass="88808" checksum="9376D8DD9F363FF7" modified="2020-10-07" version="1">MRCLRICWLREGCKGLQSGIQVLSAIAIDINSHVGKKLGNVVTDLEAQLNLPWFVFFIGSPSRWNSTMSLSVRPQRRSLVVRINRSQSFAGVNSTQDKSFRNFPAFSTPTVSRKSGSRVSRMFSMSHKSPPPKVPQPERLDEVYEALKKGLSAYLEVHQQELEKLSIQIRESKRNSRLGFLYDLDKVRHAGKEAENPRDSVRQDRIAPSCISSVSEHKMLSHLILDLSPLPLPLPSDLADVGSVIDELYEAYCIQRRLRDGAHNMVKAYTAGSPGSKEARESLAEAGKGYKEYTENMCLLESELESQLGEFHIKMKGRRGQTTSAIFKSTLSGIRNDTMSRLEWNVYCCSPVDRNLARLKHRLHNDIFMRYGRQRWKLRGRIEANSKQVWDSEEMIFLPLITEFLSIKVTELKSLASHVVVGNVSCETKDLFAALPQVVAVDINDLGTIKLSLEVNWNPFDKEDQPSSASTVNKTSTVNKRFSTYNQSPPDTPSMREQAFYNMLRQQEELENGAAWSISSESSDDSSSPQLSGGARHSLPRPIVQPAVQATAPAIEIAFTHPEDKEPAIREEAAMANGHVPYARTLSQISEASVDSPIAEERECPVPKEAGPWGAPPLETNSTPPSVGSPLDAQICEDEEQEDEPDGAAPVGEESEDVPGPAPEVQQPKSSLAPSASGQLQPSGISEPPRPKPVDSGLEEAINTLASALDDYRGQFPELQVLEQELKHLEEILLSRNQQAKESIEQLVYLCQTDKEAVRDAAKQSLLMCGDDGKSAHRRLEESLDNLPRIFAPGSMASTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7C3</accession>
    <name>A0A6I9Z7C3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="15">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BAP1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14 15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the PR-DUB complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C12 family. BAP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013931781.1">
        <property type="nucleotide sequence ID" value="XM_014076306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7C3"/>
    <dbReference type="GeneID" id="106557150"/>
    <dbReference type="KEGG" id="tsr:106557150"/>
    <dbReference type="CTD" id="8314"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000002">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.860:FF:000010">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF28">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52049">
        <property type="entry name" value="ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 14 15" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 14 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="17">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="273"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="371"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="492"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="609"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="714"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="493"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="14">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="14">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013931781.1"/>
        </source>
    </evidence>
    <sequence length="740" mass="82577" checksum="1A1CAA51FD74135B" modified="2020-10-07" version="1">MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDETSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCNSVDLGPTLSRMKEFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGISAVRTMEAFHFVSYVPIKGRLFELDGLKVYPIDHGPWAEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRMKYESKLHILKVNRQTVLEALQQLIRVTQPELVHTQKSQELQTPEEPRTANSKSSTPLETGRTQVIPESSQAEGTEDSRAQDLPASTQSPPSKAKLSSKPHVSSINGALSAPNPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVPQHQQYSDDEDDYDDDEDEEVCNTNPAISLIPCFLLPLALDSYKRKGLVKQEHLMGSGDTQLSMLQPNTINVLAEKLKETQKDLSIPLSIKTSSGGTAVAVVAHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEEESLLRVDCIRYNRAVRDLGPIISTGLLHLTEEGVFCPLTVTEGTKTSPPSNKSNEEVQIIVKAEAVDSTEPRDSKEKTGIGKSTDPPAGEKYVPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLPSLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WY13</accession>
    <name>A0A6I9WY13_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Centromere protein H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CENPH</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CENP-H/MCM16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907041.1">
        <property type="nucleotide sequence ID" value="XM_014051566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WY13"/>
    <dbReference type="GeneID" id="106537436"/>
    <dbReference type="KEGG" id="tsr:106537436"/>
    <dbReference type="CTD" id="64946"/>
    <dbReference type="OrthoDB" id="2274804at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040034">
        <property type="entry name" value="CENP-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008426">
        <property type="entry name" value="CENP-H_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48122">
        <property type="entry name" value="CENTROMERE PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48122:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05837">
        <property type="entry name" value="CENP-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centromere protein H C-terminal" evidence="10">
        <location>
            <begin position="49"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013907041.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="29377" checksum="8B9B46D991EBA0EF" modified="2020-10-07" version="1">MESRDEAGCPEIGDKAAPVAAFGKPTEGVTEEGARLRFNEDRMPDLLKLYRFKEQISQHLMEYKTIINSAAAEKIPDQDRNEKILEGSIEELERDLEEIRLSYQNKALALQRIQITDALRTKLKNEDDDSKFIWDTIKHIMMLSTAILKSKQQSRELEEKLNEVKKSRLELKRAGECKLAQIHDMKRKQKKDLENMEVGEMLKKVRKNVQKEIQMTTLIQNIFQNLITGSGVNWAKDPALKRIVLQLENNVTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYH2</accession>
    <name>A0A6I9WYH2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chromatin target of PRMT1 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106537561</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907206.1">
        <property type="nucleotide sequence ID" value="XM_014051731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYH2"/>
    <dbReference type="GeneID" id="106537561"/>
    <dbReference type="KEGG" id="tsr:106537561"/>
    <dbReference type="OrthoDB" id="446014at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052656">
        <property type="entry name" value="CTOP_PRMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48426">
        <property type="entry name" value="CHROMATIN TARGET OF PRMT1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48426:SF1">
        <property type="entry name" value="CHROMATIN TARGET OF PRMT1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01218">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Chromatin target of PRMT1 protein C-terminal" evidence="3">
        <location>
            <begin position="51"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013907206.1"/>
        </source>
    </evidence>
    <sequence length="141" mass="14614" checksum="DF8CE4294CECCC04" modified="2020-10-07" version="1">MGQRGLPRGAMRGGRGARGLLRGGIPLRGYSLLRGGRGLGPRMGLRRGGMRGRAGPGRGGLGRGAMGRGGLGGRGRGMAGRARGGFGGRGRGRGRGRGTTRPALTKEQLDNQLDAYMSKTKGHLDAELDAYMAQTDPETND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X004</accession>
    <name>A0A6I9X004_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amyloid beta A4 precursor protein-binding family B member 1-interacting protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">APBB1-interacting protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">APBB1IP</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MRL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908564.1">
        <property type="nucleotide sequence ID" value="XM_014053089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X004"/>
    <dbReference type="GeneID" id="106538571"/>
    <dbReference type="KEGG" id="tsr:106538571"/>
    <dbReference type="CTD" id="54518"/>
    <dbReference type="OrthoDB" id="6235964at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01259">
        <property type="entry name" value="PH_APBB1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16137">
        <property type="entry name" value="RA_MRL_RIAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000124">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 1-interacting protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000048">
        <property type="entry name" value="Ras association (RalGDS/AF-6) and pleckstrin homology domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039665">
        <property type="entry name" value="PH_APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF14">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B MEMBER 1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="12">
        <location>
            <begin position="173"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="303"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="435"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="545"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="552"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="589"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013908564.1"/>
        </source>
    </evidence>
    <sequence length="661" mass="74182" checksum="4E2B98EB432C4014" modified="2020-10-07" version="1">MEEACDDIDQMFSDLLGEMDLLTQSLEVEAMPISHQKSTDAEFNFAVGFKDLNESLTTLEDTDLDTLMADLVADISEVEKTTLKEPKNSSYNEQDAIMQPPAKADPICGTQSVLNNIPSPYEDTLPAPPAEMLMPLPPPPAIPLKPLTQEAQEQAKTDKIKLALEKLKEAKVKKLVVKVHMNDNSTKTLMVDERQVARDVLDNLFEKTHCDCNINWCLYEIYPELQIERFFEDHENVVEVLSHWTRDSENKILFLEKNEKYAIFKNPQNFYLSNKGKNEAKEMNEKSKEALLEESFCGTSVIVPEVEGALYLKEDGKKSWKRRYFLLRASGIYYVPKGKTKTSRDLACFIQFENVNVYYGIQYKMKYKSPTDYCFVLKHPQIQKESQYIKYLCCDDKQALHHWVTGIRIAKYGKALYDNYIGAVQKSELASRLAKPGNTKSIASGESSSKGSSHANGQIPQNINLNTENTAETGKSAVMPKIQINVKRPDDTFNVGQASSLPVQQVKQIRKPSGLHPPPPPQRRSSAITASLDLSAIAERENPSFLPNYEEFPPPPEFIPLPPNLEDLPPPPPPPPSPPLPDYYESPPDFTPPPPPSFPAPSPPPATKKKPPPPPRRQEEGACPVVRPKQSGSISRGHASEQGDFMSDLMRALQKKRSESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0D4</accession>
    <name>A0A6I9X0D4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neuropeptide Y receptor type 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NPY-Y2 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106538657</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908699.1">
        <property type="nucleotide sequence ID" value="XM_014053224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0D4"/>
    <dbReference type="GeneID" id="106538657"/>
    <dbReference type="KEGG" id="tsr:106538657"/>
    <dbReference type="OrthoDB" id="9046662at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001358">
        <property type="entry name" value="NPY2_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF22">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01014">
        <property type="entry name" value="NRPEPTIDEY2R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="102"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013908699.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="47219" checksum="2B1FDBDF1CA98C0A" modified="2020-10-07" version="1">MYSASQFLRILQDKHTSVILSTHKYKKIGMGLLDQVNSTIILEERISEKKSQSRWHADSLVTPSQGFQGGSSEIMMDSTKILGVQIILIMAYSLIILLGFIGNSLVIYMIVRYKTMRTVTNFFIANLALADLMVDTCLPFTLAYTLLDEWKFGAVLCHLVSCAQALSVHVSILTLTVIALDRYRCIVFHLDSRISKKISFTIIAITWLVAALLASPLAIFREYRYEEIPSINLRIAVCSEKWPSGSRDATIYSLSMLILQYVFPLSIICYAYIRIWFKLKSHISPSATNETHCRRKKTTKMLFMVVVVFAVSWLPFHAFQLAADLDMALALRDYKLLYTVFHVIAMCSTFANPLLYGWMNKNYRNGFLMFFRCQKKPERFYAEGSVRGRSFTFKATTLNGSIKHSTGDGQLPTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3N0</accession>
    <name>A0A6I9X3N0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Collagen alpha-3(IV) chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106538452</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908452.1">
        <property type="nucleotide sequence ID" value="XM_014052977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3N0"/>
    <dbReference type="GeneID" id="106538452"/>
    <dbReference type="KEGG" id="tsr:106538452"/>
    <dbReference type="OrthoDB" id="9398093at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1082">
        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026925870" evidence="4">
        <location>
            <begin position="27"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="73"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="263"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013908452.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="34953" checksum="835B7B54E0B4DC30" modified="2020-10-07" version="1">MEGLVRPKSWHLGLAVIYMLALGGWEQDGFPTAVAAKNCICNSKNQCFCDGIKGSKGEKGLPGYPGLPGQMGFHGPEGPPGPQGPKGFSGPVGPAGVKGSRGKPGIPGYSGPPGLPGEPGNAGPHGAPGIPGCNGTKGEPGLPGLLGQGGFPGLPGSKGIKGEKGASVNGFNQSYSSSKGNPGLPGVPGFQGKPGLQGLPGTIGPQGSSGFPGTVGRAGPQGPKGIMGINVIGSKGIKGDCGLIGPPGPSGTVIITLTGAENTTGLKGDKGDQGSPGHPGPSGEPGLYGDSYTIKGEPGNPGHQGRPGRDGGPGSPGLWGEKGEKGLPGLPGRFGTKVRKKKREREIFFLIKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5J0</accession>
    <name>A0A6I9X5J0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Autism susceptibility gene 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AUTS2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013909082.1">
        <property type="nucleotide sequence ID" value="XM_014053607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5J0"/>
    <dbReference type="GeneID" id="106538948"/>
    <dbReference type="KEGG" id="tsr:106538948"/>
    <dbReference type="CTD" id="26053"/>
    <dbReference type="OrthoDB" id="10060000at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023246">
        <property type="entry name" value="AUTS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429:SF5">
        <property type="entry name" value="AUTISM SUSCEPTIBILITY GENE 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429">
        <property type="entry name" value="FIBROSIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15336">
        <property type="entry name" value="Auts2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02044">
        <property type="entry name" value="FIBROSIN1LPF"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="637"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="664"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013909082.1"/>
        </source>
    </evidence>
    <sequence length="715" mass="78308" checksum="DEB8DCD927C03BEC" modified="2020-10-07" version="1">MIKSSWFYVRFKYAEKLNPGQNSCRDSDSESASGESKGFHRSSSRERLSDSSAPSSLGTGYFCDSDSDQEEKASDASSEKLFNTVTNKDPDLGVVTLTENEDQEARPLALPKISGLERSQEKSQDGGKEPSLEPVVPQDLCPQVPQPLVPPRLVPPTEARSPEPPAVHPAGSSYSEAPEKQPALEEAQFPTLPPSQIQHPPRPPSPGQPAHPNRPPSQLRPPSRSLAQQLSAYNSSSLSLNSLSSSRSSTPAKSQPPPPPPPHPPIAHHPSASPFPLSLPNHSPLHNFTPALQPSNHTHHPNMFAPPTALPPPPPLTSGTLQVPAHPAGTAYSEQDLLRQELNTRFLASQSADRGASLGPPPYLRTEFHQHQHQHQHTHQHTHQHTFTPFPHAIPPTAIMPTPAPPMVRNPGRNFDKYPTKVDPFYRHSLFHSYPPAVSGIPPMIPPTGPFGSLQGAFQPKTSNPIDVATRPGALPHTLLQKDPRLTDPFRPMLRKPGKWCAMHVHIAWQIYHHQQKVKKQMQSDPHKLDFGLKPEFLSRPPGPSLFGAIHHPHDLARPATLFSATSAAHPAGPTFGPPPHHSNFLNPAAHLETFNRPTTFTSLAAMSSNAFGGLGNHAVTPNAMYGHKDCPGMQSYSNPHEPWNRLHRTPPSFPTPPPWLKPGEQERSASAAAHERDRDVDKGDASLSKDDKERSSNFILQNFVATKYLELILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5W3</accession>
    <name>A0A6I9X5W3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UPF0258 protein KIAA1024-like homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106539380</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MINAR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909641.1">
        <property type="nucleotide sequence ID" value="XM_014054166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5W3"/>
    <dbReference type="GeneID" id="106539380"/>
    <dbReference type="KEGG" id="tsr:106539380"/>
    <dbReference type="OrthoDB" id="8920945at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039706">
        <property type="entry name" value="MINAR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009626">
        <property type="entry name" value="MINAR1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31530">
        <property type="entry name" value="MAJOR INTRINSICALLY DISORDERED NOTCH2-BINDING RECEPTOR 1 MINAR1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31530:SF4">
        <property type="entry name" value="MAJOR INTRINSICALLY DISORDERED NOTCH2-BINDING RECEPTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06789">
        <property type="entry name" value="MINAR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Major intrinsically disordered Notch2-binding receptor 1-like C-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909641.1"/>
        </source>
    </evidence>
    <sequence length="86" mass="10207" checksum="B4F967EBB5AAB1AE" modified="2020-10-07" version="1">MIDKALGKHVTPVTLKSTIKSNPLYDGILADDCWEKRRHNPSWTVQDYDRHSNTSLPYYIKNYKEETLEVFHSNPLLKQETLRRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7S1</accession>
    <name>A0A6I9X7S1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase ZSWIM2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ZSWIM2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910321.1">
        <property type="nucleotide sequence ID" value="XM_014054846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7S1"/>
    <dbReference type="GeneID" id="106539923"/>
    <dbReference type="KEGG" id="tsr:106539923"/>
    <dbReference type="CTD" id="151112"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16486">
        <property type="entry name" value="mRING-H2-C3H2C2D_ZSWM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16494">
        <property type="entry name" value="RING-CH-C4HC3_ZSWM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039903">
        <property type="entry name" value="Zswim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZSWIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540">
        <property type="entry name" value="RING FINGER AND SWIM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="7">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="141"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="224"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="339"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013910321.1"/>
        </source>
    </evidence>
    <sequence length="519" mass="59471" checksum="8D575622E2125013" modified="2020-10-07" version="1">MSRRRVSASPELSWRQDQALSTTMRIVRELGPTAFILQEEDRRAAELRVSLGNPHSCSCSVFLKEKDLCKHICWLLLKKFKLPRDHEYALQIGLLEREINYLLQSPQLSPQVKVIAHSLKELKTVDDGYVKQKNIDNEDICPVCQETLLKKMLPVTYCRFGCGNNIHIVCMKILIDHQRDLEKNSLVKCPLCRENFAPLKLILEEFRNCKKLVTAAEKQSLNKHLGIPCNNCRVLPIEGNCYKCTECSDYHLCHGCFQSFCHPSHTFSFREKRNQRWKSFEPKVQLSTTEENPKINPEEKFIEEKNKEQFSCIPQQVVNSIPNMVIRKGSPLLDPGLQCRLCLKIFCLGQVTRTLPCNHKFHRKCIDRRLQKENACPIDDHVVYNPLTWETAPSKDKVNQLGSQAKHLLAKQPGLDLFIPGTGLSIKQIKPHHVTEVSQSSFQGNWNKKKNSAETEQNLTLNGFSYPCTRDAGSSLLNTQKPRHLKFSRHMKGLVLIPKAHADTVTDKVVGTNLRLYAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8K1</accession>
    <name>A0A6I9X8K1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106540150</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910661.1">
        <property type="nucleotide sequence ID" value="XM_014055186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8K1"/>
    <dbReference type="GeneID" id="106540150"/>
    <dbReference type="KEGG" id="tsr:106540150"/>
    <dbReference type="OrthoDB" id="9044658at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910661.1"/>
        </source>
    </evidence>
    <sequence length="620" mass="70789" checksum="A490712E35E3ADCC" modified="2020-10-07" version="1">MIHTFPKELCLHPGQTKNMIESLFSQHGICSASIQRIPYLTHWDKFHEMIDKSTNIYNGIADEKIKTFIVSGDSLTISWVTTIMFFVCPGCKQSSLGKVWIMTAQVDYLLTGIQRKWDRQFFDGAIFFTIHSKQIPGHNEFLKFIKPPWIQEDGFFPLFWEQVFDCSLPSSGLTVVDGNICTGEDKLEDIPGSLFEVQLTGHSYGIYNAVYILAQALHALFLLQSSHGDLMKPKLLHYEDAQPWKRQKVGKVDPDALKGQELVINKEMIIWHRSFNQDSFRFLPWSQLSFLHAPFFHFSQIRHENTGQEIEVGGSATKKLITNLKPQTSYRFSLVGQRSEKGDLQQKVVVSTAANMLSRKPEVSYRHEIDGNVIVTLPDVRTSDPPVQAYYIVVLPLKKTKSGQFQNPYNSPEEMDLEELIQQISRLQRRSLRHSRQLDPHKAYIAARVHVLPSIFTLGDMKRYDNFENKALEAGQKYVIFILAVLQTNEPMYAASPFSDPIQMDSSDPQPKIEGEEGLIWVIGPVLAVVFIICIVIAILLYKNKPDSKRKDSEPRIKCLLNNAEITPHHPKDPVEMRRINFQTPGNQLHATLPLLLDGCMLLIPLHALAKFCSQRHTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X915</accession>
    <name>A0A6I9X915_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cationic amino acid transporter 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106540282</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910821.1">
        <property type="nucleotide sequence ID" value="XM_014055346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X915"/>
    <dbReference type="GeneID" id="106540282"/>
    <dbReference type="KEGG" id="tsr:106540282"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF20">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="11">
        <location>
            <begin position="466"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910821.1"/>
        </source>
    </evidence>
    <sequence length="546" mass="59823" checksum="7B684AD104D1D26A" modified="2020-10-07" version="1">MLVINNSQEGAESQLGYSCRKGPHPPKHNRASRTEWHEATGARRAQKRGFNLPGLFLPSGGKLKTRRPSPPFPPRSNHRRRSFLLWLPGLLSFGVSESALVNKIFTAINLLVLSFVIVAGFVKGDVKNWNLSAEDCQNRTETLLESGQKVRFGNGGFFPFGFQGVLAGAATCFYAFVGFDCIATTGEEARNPQRSIPISIIISLIICFVAYFGVSASLTLMVPYFLVDKESPLPDAFKVVGWEPARYVVAIGSLCALSTSLLGSMFPMPRVIYAMAEDGLLFRFLFRVHARTKTPLVATVVSGIIAALMAFLFELKDLVNLMSIGTLLAYSLVAICVLILRYQPELFRFSKDLEMLEINGSEEEKMTINSTVGDGQNAFKEKFSCRSLFFPAEDNPTHVSGRIVYISTTAVAVTITALCGILAHEWEALLEGSVGWVIACVILLGILLAGTAVIWRQPESKTRLTFKVPGLPLLPLFSILVNIYLMMQLDAGTWARFAVWMAIGFAIYFGYGLQHSEEGQGAQQSPSAADKGLPSAHSELSQELAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9N4</accession>
    <name>A0A6I9X9N4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC106541401</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2014" name="Mol. Biol. Evol." volume="31" first="2836" last="2846">
            <title>Parallel evolution of tetrodotoxin resistance in three voltage-gated sodium channel genes in the garter snake Thamnophis sirtalis.</title>
            <authorList>
                <person name="McGlothlin J.W."/>
                <person name="Chuckalovcak J.P."/>
                <person name="Janes D.E."/>
                <person name="Edwards S.V."/>
                <person name="Feldman C.R."/>
                <person name="Brodie E.D.Jr."/>
                <person name="Pfrender M.E."/>
                <person name="Brodie E.D.III."/>
            </authorList>
            <dbReference type="PubMed" id="25135948"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cell membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912319.1">
        <property type="nucleotide sequence ID" value="XM_014056844.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541401"/>
    <dbReference type="KEGG" id="tsr:106541401"/>
    <dbReference type="OrthoDB" id="2984333at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000006">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000005">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000003">
        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF237">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 3 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 17" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 17" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="14 17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <keyword evidence="8 17" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="862"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="950"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1190"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1229"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1306"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1427"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1509"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1539"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1564"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1626"/>
            <end position="1654"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1729"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="20">
        <location>
            <begin position="142"/>
            <end position="433"/>
        </location>
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    <feature type="domain" description="Voltage-gated Na+ ion channel cytoplasmic" evidence="22">
        <location>
            <begin position="557"/>
            <end position="701"/>
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    </feature>
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            <begin position="751"/>
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            <begin position="990"/>
            <end position="1184"/>
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            <begin position="1188"/>
            <end position="1459"/>
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        <location>
            <begin position="1508"/>
            <end position="1762"/>
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    <feature type="domain" description="SCN5A-like C-terminal IQ motif" evidence="23">
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            <begin position="1874"/>
            <end position="1904"/>
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            <begin position="435"/>
            <end position="465"/>
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            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
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        <location>
            <begin position="479"/>
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            <begin position="494"/>
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            <begin position="564"/>
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            <begin position="583"/>
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            <end position="1945"/>
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        <location>
            <begin position="1950"/>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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            <dbReference type="RuleBase" id="RU361132"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06512"/>
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            <dbReference type="Pfam" id="PF24609"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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            <dbReference type="RefSeq" id="XP_013912319.1"/>
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    <sequence length="1974" mass="225778" checksum="35F472D52800FE46" modified="2020-10-07" version="1">MAHALHAQPGPDCFFQFTQESLAAIEQRITEEKAKQKVEQEQKEEDHDESKPKPNTDLEAGKSLPFIYGDIPSRFVSQPLEDLDPYYANKKTFIVLNKGKTIFRFSATPALYMLSPFSTLRRISIKILVHSYPFTTEHNPTLFSIFIMFTILTNCVFMTLKDPPKWSWMVEYTFTGIYTFESLIKIFARGFCIDKFTFLRDPWNWLDFLVISFAYITEFVKLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTLFCLSVFALIGLQLFMGHLRHKCLYWPNNTSPEDPRFKEYYNGTELLWSKYMENKDHFYYLEGAKDALLCGNSSDAGQCPEGYICVPYGRNPDYGYTSFDSFSWAFLSLFRLMTQDYWENLYQQTLRAAGKGYMFFFVVVIFLGSFYLINLILAVVAMAYDEQNQATIEEALRKETEYQQMLEHLKRQQEEAQALMAAAAYKDFRDDGTLGRLSETSSELSSSKSAKERRNRRKKRRQRELSVGEPGGNNKMFPKSESDSSIRRKGFRFSLEGNRLAYESRVISPYQSILFPTRSNSRASFSSFKSPTVEGGSDADSEHSTFEENGSRNGSYFVVRSHSERRSSNISQTMFPMNGKMQSSVDCNGVVSLVGGPPVLLSPTGQLLPEVIIDKATTDDSPTTSEMENKKRQSSSFQISMDLLEDPTIRERAMSIASIITNTMEELEESRQKCPPCWYKFAHKYLIWNCSDRWLQIKKIIHLIVMDPFVDLGITICIILNTLFMSMEHYPIDDSFSSILKNGNLVFTGIFTAEMVLKIVAMDPYYYFQEGWNIFDSIIVTLSLMELGLQNVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYDMCKCKISEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVSGQPLCLTVFMMVMVIGNLVLLNLFLALLLSSFSSDSLAAPEQETEANNLQIAISRIQRGINYIKRKICEFVQIVFLQRCKATSGLSAADQQNDKEDQCIPNHTIVEINQTFGYQRPRMTTSCVDNTDHMSFINNPNLTVTVPIAVGESDFEHFNTEELTSISEVEETKEKTSLCSSTEGSTIIFASVGDKESDAAAKGPPQPKPCFTDGCVQKFKCCQIDIESGKGKCWWNLRKTCFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYIFILEMLLKWVAYGFQAYFTNAWCWLDFVIVDVSLVSLIANALDYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGTFFECVNKTDGVRISHLIVPFKNVCETLDYARWRNVKVNFDNVAAGYLSLLQVATFKGWMEIMYAAVDSTGIEEQPQYEHNLYMYLYFVGFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPSNKIQGFVFDFVTKQAFDIGIMILICLNMITMMVETADQDTSVEDILYWINLIFIVLFTAECLLKLIALRYYYFTIGWNIFDFVVVIFSIVGMCLSKIIEKFFVSPTLFRVVRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSQFAYVKREAGIDDMFNFETFANSMICLFQITTSGGWNYLLYPSLNKEPDCDPKKVHPGSSVLGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLGEDDFEMFYEVWEKFDPGATQFIELSKLFDFAASLEPPLLIPKPNKVQLIAMDLPIVSGDRIHCLDILFAFTKRVLGESDEMDSLRVQMEDRFMAANPSKVSYEPITTTLKRKLEEQSARVIQRAFRHYRLRKPVCNTDVQYLNRNIFPCKAEMEFDKFSLSLTLEKTERSSSTTSPPSYDSVTKPDKYEQEKSEKEEKGKDDKDYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9T4</accession>
    <name>A0A6I9X9T4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MCM6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013910517.1">
        <property type="nucleotide sequence ID" value="XM_014055042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9T4"/>
    <dbReference type="GeneID" id="106540055"/>
    <dbReference type="KEGG" id="tsr:106540055"/>
    <dbReference type="CTD" id="4175"/>
    <dbReference type="OrthoDB" id="1744952at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.870:FF:000001">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000091">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000004">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF73">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="346"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="663"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="666"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910517.1"/>
        </source>
    </evidence>
    <sequence length="822" mass="93212" checksum="E78D71B86F0F0452" modified="2020-10-07" version="1">MDLVAGGEAGLAAAQKQLRDEVAEKCQKLFQDFLEQFQNTDGEVKYLQDAEELIRPERNTLTVSFEDLEQFNQQLATTIQEEFYRVYPFLCRAVKTFAKDHGTVPPNKDFYVAFQDLPTRHKIREMTSARIGSLIRISGQVVRTHPVHPELVSGTFLCLDCQTVIKDVEQQFKYTQPNICRNPVCANRKRFLLDTNRSRFVDFQKVRIQETQAELPRGSIPRSVEVILRAEAVESAQAGDKCDFTGSLIVVPDVSQLSTPGVRAETGSRVTGKEGYESEGVRGLRALGVRELSYKLVFLACYVAPTNPRFGGKELRDEEQTAENIKNQMSVMEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEVKRGILLMLFGGVPKTTAEGTSLRGDINVCVVGDPSTAKSQFLKHVEEFSPRAVYTSGKASSAAGLTAAVVKDEESYEFVIEAGALMLADNGVCCIDEFDKMDMRDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPVGGRYDRAKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIDESIDRIYTLDEIRRYLLFARQFKPKISKESEDFIVEHYKRLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCSDEVHPKHVKEAFRLLNKSIIRVETPDINLDQDEEQSVEEPEDQDGVAREEDNVLTNGLVNGTSDPVDDSNKDVAPKASLRLSFPEYQRISNLIVMHLRRSEEENDAALKKSELINWYLKEIESEIDSEEELINKKKVIERVIHRLTHYDHILIELTHSGLKDSVEVQSFDEDPYLVVNPNYLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XB04</accession>
    <name>A0A6I9XB04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Sterile alpha motif domain-containing protein 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC106541763</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912769.1">
        <property type="nucleotide sequence ID" value="XM_014057294.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912770.1">
        <property type="nucleotide sequence ID" value="XM_014057295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XB04"/>
    <dbReference type="GeneID" id="106541763"/>
    <dbReference type="KEGG" id="tsr:106541763"/>
    <dbReference type="OrthoDB" id="434324at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052268">
        <property type="entry name" value="SAM_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20843:SF0">
        <property type="entry name" value="PROTEIN AVEUGLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20843">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="44"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912770.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="13493" checksum="3A828205FA521DF6" modified="2020-10-07" version="1">MIPMESNAKRGLGNLNGMEESQAEPAGLEKGSKEEEARRPVAQWTAAEVCAWLRGKTLGTPGSPLLEAANSHAISGKALLRLSEETLERMGIAPKSLREELLQEILLLRIQQEMEDLLDISDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEJ6</accession>
    <name>A0A6I9XEJ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thioredoxin domain-containing protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TXNDC16</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912287.1">
        <property type="nucleotide sequence ID" value="XM_014056812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEJ6"/>
    <dbReference type="GeneID" id="106541384"/>
    <dbReference type="KEGG" id="tsr:106541384"/>
    <dbReference type="CTD" id="57544"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040090">
        <property type="entry name" value="TXNDC16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057645">
        <property type="entry name" value="TXNDC16_3rd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22699">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22699:SF1">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24510">
        <property type="entry name" value="TXNDC16_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TXNDC16 third thioredoxin-like" evidence="3">
        <location>
            <begin position="9"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="161"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912287.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="67302" checksum="4BAEAD97DEB9B727" modified="2020-10-07" version="1">MLDLAKIKVEVAEDPEKISTTHLQLGLPLIFILSQEETLEADKRIAESVAWQLLGKAGLLLLSRNSVGLDVSSRYNVAIKTVEEDMPVKYLTLKDADEIVALVRNGEQSNPSLDDKAEEVYEEEEEEEGVENENNEQEIQDDEVVESAVRDRKRELSLKHIHTLTEESFLSILAETNHTAVLFYASWEAVSLVVMQSFLEVAAQLKGTPEISLARVNCWDWPHLCLRENVTQFPIMKMYTKEGAWLAYSGMWETKEMMKFIELSRNSCPVRLMTPEEIEEYLTDKTSPHHTVSVLGIFDSSMSEAREAFIEAGRILNGYVTTGIYYEEDATVLAHKYNVALPALLFAKPSTQQRNAFQLSQYNTQDIVKVIRHALLETFPEITVENLPDYFQMKKPLLILFSDGIPGEKEVEEMKHVAVGEDHKTFITCWLNLKNTPVGRSILKAYFGNSVVPVPLLLWISLHSGGHVFVFPSDQSISETNILAWIEKLKTKQEAPRATLSEKEWKPRLPAYNFLSMMEPTLPEFTIYTQESISIRQEEILGEKKAEAAVTKKPTKDSTMEELKPKKPTGRALRRAAPHLGAKEKQAKHHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XF99</accession>
    <name>A0A6I9XF99_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WASH complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 53</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CCDC53</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC53 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914339.1">
        <property type="nucleotide sequence ID" value="XM_014058864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XF99"/>
    <dbReference type="GeneID" id="106542981"/>
    <dbReference type="KEGG" id="tsr:106542981"/>
    <dbReference type="CTD" id="51019"/>
    <dbReference type="OrthoDB" id="268027at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000025">
        <property type="entry name" value="Putative WASH complex subunit CCDC53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019309">
        <property type="entry name" value="WASHC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13015">
        <property type="entry name" value="PROTEIN AD-016-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13015:SF0">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10152">
        <property type="entry name" value="CCDC53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013914339.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="20930" checksum="DB1FCFEC10075DC7" modified="2020-10-07" version="1">MDEDGLPLVGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSTTCEEKLSALSLRIQQIETLLNILDAKLSSVPGLEDEISNTSVMNGSASQTLVEPQTTNISPNVETSVPELGQQKTNGTDVITVAKDPHYARYLKMVQVGVPVMAIRNKMISEGLNPDLLETPDAPVPAWGDETNKGESSDSESSFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFI1</accession>
    <name>A0A6I9XFI1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Calcium-dependent secretion activator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CADPS2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912931.1">
        <property type="nucleotide sequence ID" value="XM_014057456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFI1"/>
    <dbReference type="GeneID" id="106541895"/>
    <dbReference type="KEGG" id="tsr:106541895"/>
    <dbReference type="CTD" id="93664"/>
    <dbReference type="OrthoDB" id="10063282at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990504">
        <property type="term" value="P:dense core granule exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033227">
        <property type="entry name" value="CAPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057457">
        <property type="entry name" value="CAPS_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12166">
        <property type="entry name" value="CALCIUM-DEPENDENT SECRETION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12166:SF7">
        <property type="entry name" value="CALCIUM-DEPENDENT SECRETION ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25341">
        <property type="entry name" value="C2_CAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="353"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="491"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912931.1"/>
        </source>
    </evidence>
    <sequence length="561" mass="63413" checksum="6B201765FEC874CE" modified="2020-10-07" version="1">MLDPSSSEEESDEVLEEERRDLLGVSGGSGPSNTSPRALPAASRDSGRQGSGGGVATRSVSPSPSLRSEGREEQERLQREEREKRLRLQLYVFIVRCIAYPFNAKQPTDMARRQQKLNKQQLQVVKERFQAFLNGETQIVADEAFCNAVRSYYEVFLKSDRVARMVHSGGCSANDFRDIFKKNIEKRVRSLPEIDGLSKETVLSSWIAKYDAIYRGEEDLYKQSTRMALSAVSELILSKEQLYEMFQQILGVKKLEHQLIYNACQLDNADEQAAQIRRELDGRLQMADQMAKERKCPEFIAKEMEHMFIEELRSSVNLLMANLESLPVSKGGPEFKLQKLKRSQNSAFLDIGDENEVQLSKSDVVLSFTLEIVIMEVQGLKSLAPSRIVYCTMEVEGGEKLQTDQAEASRPQWGTQGDFTTTHPRPVVKVKLFTESTGVLALEDKELGRVVLYPTSNSSKSTELHKMLVPKNSQDSDLRIKLAVRMDKPPHMKHCGYLYALGQKVWKRWKKRYFVLVQCEAQKALVCAGVERHHMDNGSGLPVEASEYALNKPYAISNAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFN4</accession>
    <name>A0A6I9XFN4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106539584</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909868.1">
        <property type="nucleotide sequence ID" value="XM_014054393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFN4"/>
    <dbReference type="GeneID" id="106539584"/>
    <dbReference type="KEGG" id="tsr:106539584"/>
    <dbReference type="OrthoDB" id="5967130at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF253">
        <property type="entry name" value="OLFACTORY RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909868.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35940" checksum="5D33DB2622803E52" modified="2020-10-07" version="1">MAERKEENQTEITEFHLMGFVGLGDLQIPLFCLFLVIYIATMVGNILIILLVVTNPHLHTPMYFFLSNLSCLEVCYSCNILPIMLANVLYGGQTTVSVTGCIMQHCLFCLLVIFECCLLLAMSYDRYVAICKPLHYTTLMNDKVCIQLIIGSWIHSLFVMSIVLYLMCQLKFCGPNEIHHFFCELNPISKLACSDIHLIELVTISLSALSILFPFFLILVSYIFIISTIMRMSSVSGRKKAFSTCSSHLIVVSIYFSTLIIVYMLPKTDGLREINKVLCLFYTIVTPLFNPLIYSLRNREVTEALKKLIATLFLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFX8</accession>
    <name>A0A6I9XFX8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC106541769</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106541769</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912777.1">
        <property type="nucleotide sequence ID" value="XM_014057302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFX8"/>
    <dbReference type="GeneID" id="106541769"/>
    <dbReference type="KEGG" id="tsr:106541769"/>
    <dbReference type="OrthoDB" id="9046590at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="498"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912777.1"/>
        </source>
    </evidence>
    <sequence length="808" mass="89881" checksum="E105E4DB03DD9E07" modified="2020-10-07" version="1">MLTILQNVISDCCHLLLFLLVAIIFVPATIWAFKKVKKIQACNKKQSSVLGKNKYHKHRSKEDKGYKLSHRTKTTSHSNLKAPTNVQIGITPQNEVIHWVSQQRSTGQRSRSRTPPSTSPKRHQTPQKPPPVRVRSPGPGQRSHSRTPPSTSPKGRQTPPKPPARARSPRPCTQTPLPSSKDLLPPPFIICQTTSHSNLKAPTNVQIGITPQNEVIHWVSQQRSTGQRSRSRTPPSTSPKRHQTPQKPPPVRVRSPGPGQRSHSRTPPSTSPKGRQTPPKPPARARSPRPCTQTPLPSSKDLLPPSFIICQATSHSSLKEPTNGNLSSMTPEEELTEVLNLLAQNESLLTKQPGRPRTPKKKEKDVRSKKPSTSKDGAGRSGSPKGKPAETQISLMLDRQTHRLSWADDMTEVPRESGDLPSFAQTSFLDTTFMCQNQLELPCIHQAQIELQQESFKLAKSPLKSQCLAHAIVESLDTEQATRDLYMCLAKGLENGHSQPSVEYPICLLCGRCAPYCPHPHPQHGPCLLVYPRLSVQDGEVYMNLGFLLKIKRHEANRWGLVQGKDASKLQHSKGHPSKQERSQSRSPSRSRSRRNAHEAAPPSRAAERPSRLARQRPTEAIDLRARRHRAGPRPSRATTPRSPPRSPKMESQPRKPAPEATQPKSPQKPPNILKKLLLCIKKVWAKVRRRREPPSTKRSSRTSLQKAASSPSLVDPSAPSGFPANGISHQKQGSLRKHAVTTTHAKQGHSHGHRKMSMKVSDHESFHPHKKVDSYRAKPASSGAPKKHSRHSPPRQLQRSPSRANLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMR5</accession>
    <name>A0A6I9XMR5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Serine/arginine-rich splicing factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Splicing factor, arginine/serine-rich 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">SRSF3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons. Also functions as an adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912058.1">
        <property type="nucleotide sequence ID" value="XM_014056583.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912059.1">
        <property type="nucleotide sequence ID" value="XM_014056584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMR5"/>
    <dbReference type="GeneID" id="106541209"/>
    <dbReference type="KEGG" id="tsr:106541209"/>
    <dbReference type="CTD" id="6428"/>
    <dbReference type="OrthoDB" id="5970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12645">
        <property type="entry name" value="RRM_SRSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000352">
        <property type="entry name" value="Putative serine/arginine-rich splicing factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 17" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="19">
        <location>
            <begin position="10"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="78"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013912058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013912059.1"/>
        </source>
    </evidence>
    <sequence length="164" mass="19316" checksum="02F0A45E33FF28A0" modified="2020-10-07" version="1">MHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSRNRGPPPSWGRRPRDDYRRRSPPPRRRSPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPY0</accession>
    <name>A0A6I9XPY0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KIF3C</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912763.1">
        <property type="nucleotide sequence ID" value="XM_014057288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPY0"/>
    <dbReference type="GeneID" id="106541758"/>
    <dbReference type="KEGG" id="tsr:106541758"/>
    <dbReference type="CTD" id="3797"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000017">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF76">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="10"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="374"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="621"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="722"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="739"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912763.1"/>
        </source>
    </evidence>
    <sequence length="762" mass="87302" checksum="8539D4A789F8750E" modified="2020-10-07" version="1">MANRPRVSETLKVVVRCRPMNRKEEATGYERIMDMDVKLGQVTIRNPKASPGELAKTFTFDAVYDATSKQADLYDETVRPLIDSVLQGFNGTIFAYGQTGTGKTYTMQGIWADPEKRGVIPISFEHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLAKDQSKKMDLKENPETGVYIKDLSSFVTKNVKEIEHVMNLGNQTRSVGSTNMNEYSSRSHAIFVITVECSETGPDGEDHIRVGKLNLVDLAGSERQSKMGAQGERPKEASKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNAKTIMVATLGPASHSYDESLSTLRFANRAKNIKNKPRINEDPKDTLLREFQEEIIRLKAQLEKRGMLSKKRRRSSRRKKTIDGEGTPEGEEPEDNEDGGLEKNMKNYLKEQKEKLEEEKAAIQDDHSLVNEEKQKLLEEKEKMIEELRREQEATELLATKYKAMESKLLIGGRNIMDHTNEQQKMLELKRQEIAEQKRHEREMQQEVLLRDEETMELRETFTSLQQEVEIKTKKLKKLYAKLQAVKAEIQDQHDEYIRVRQDLEEAQNEQTRELKLKYLIIENFIPPEEKNKIMNRLYFDCEEDQWKFQPLVLTPGNSSSSSSNSNSTTGTQMKKRPTSAVGYKRPISQYARLAMSMGSHPRYRAENIMFLELDVSPPAVFEFEITQDQSEQDPRALHLERLMRLDSLMERPAASRVRKSRSWCQTPRTLPSSATQVSLSSSSQNPSIPLAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQG0</accession>
    <name>A0A6I9XQG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">FN1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7. Interacts with COMP. Interacts (via type III repeats 9-14) with TNFAIP6 (via CUB domain); this interaction enhances fibronectin fibril assembly. TNFAIP6 may act as a bridging molecule between FN1 and THBS1. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth. Interacts with FST3 and MYOC. Interacts with SVEP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916282.1">
        <property type="nucleotide sequence ID" value="XM_014060807.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106544514"/>
    <dbReference type="KEGG" id="tsr:106544514"/>
    <dbReference type="CTD" id="2335"/>
    <dbReference type="OrthoDB" id="261433at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043394">
        <property type="term" value="F:proteoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007044">
        <property type="term" value="P:cell-substrate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00061">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000004">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000006">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000018">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000099">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000417">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000622">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000017">
        <property type="entry name" value="Fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000020">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000227">
        <property type="entry name" value="Fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000300">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000306">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000317">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000336">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000364">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000382">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000433">
        <property type="entry name" value="fibronectin isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000083">
        <property type="entry name" value="Fibronectin_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF8">
        <property type="entry name" value="FIBRONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00039">
        <property type="entry name" value="fn1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00058">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01253">
        <property type="entry name" value="FN1_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51091">
        <property type="entry name" value="FN1_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0011">Acute phase</keyword>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0133">Cell shape</keyword>
    <keyword evidence="13 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="12" id="KW-0558">Oxidation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0765">Sulfation</keyword>
    <feature type="domain" description="Fibronectin type-I" evidence="19">
        <location>
            <begin position="42"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="20">
        <location>
            <begin position="168"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="20">
        <location>
            <begin position="228"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="19">
        <location>
            <begin position="281"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="19">
        <location>
            <begin position="329"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="19">
        <location>
            <begin position="372"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="422"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="529"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="622"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="720"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="810"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="900"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="987"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1082"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1171"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1264"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1352"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1446"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1538"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1626"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1718"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1807"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="2001"/>
            <end position="2089"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="19">
        <location>
            <begin position="2100"/>
            <end position="2144"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="19">
        <location>
            <begin position="2145"/>
            <end position="2187"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="19">
        <location>
            <begin position="2189"/>
            <end position="2229"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013916282.1"/>
        </source>
    </evidence>
    <sequence length="2277" mass="250823" checksum="4C9F84271DDC0EF5" modified="2020-10-07" version="1">MRLDGNVDYIKKSSICPRGYNHHEWVQTVEIAQLYGLFFSIDRCNDQDTKRSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHSSLQATGIGSASATITDVRTALYQPQPQPAPSGHCATDSGMMYILGMQWLKTQGSQHMLCTCLGNGISCQETAVTQTYGGNSNGEPCVFPFTYHGKTFYSCTSEGRQDGNLWCSTTSNFDQDKSYAFCTEQNVLVQTRGGNSNGALCHFPFLYNNRNYTDCTSEGRRDNMKWCGTTSNYDVDEKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDGVTYDVNQTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSESQTFYQIGDSWEKHAHGVRYQCYCNGRGIGEWHCQPLNTGSAGHPVQVIVTDVPIQPDSHPIQWIAPESSHVSNYILRWKPKNSGVRWKEARIPGYLNSYTISGLKPGVLYQGQLISVQNYGQKEMTRFEFTTSSTTSISSSRVTGETTVLPSVVSISESVTEITANSFVVSWVSASDTVSGFRVEYELSEDGDEPKYLDLPNTVNSVSIPNLLPGRNYIIKVYQISEEGEQDLILSTTQTTAPDAPPDHLVENVDDTSILVSWSRPEAPITGYRIVYTPSLEGSSTELNLPESATSVTLSDLMPGVQYNISIYAVEENQESAPIFIQQKTTGVLMADEIHPPQDLQFLEVGSDKITIMWRAPESAVAGYRVEVIPINRPGEHGQRLPISRHTFAEVTELLPGTTYLFKIYAVGQNTESEPLTGEQTTKLDGPTNLRIVSETPSTALVTWKPPRARIAEYHLTLQPIPDGLPKQYTVGPSAAKYLLRNLQPGSEYTVSLVAVKGHQKSNPEKGTFTTLQSAGPVPPYNTEVTETSIIVTWIPAPRLGFKLGVRPSQGGEAPREVISESGSIVISGLTPGVEYIYSINVIKDGQERTTPIVKTVITPPSPPTNLHLDSNSDTGFLTVSWEKSTTPDITGYRITTVPTNGQEGYTLEEQVKAEDTSCVFENLSPGVQYNVSVYTLKDNQESLPISETITQEVPQLTDLNFVDVSDTSIGLKWTPINTSAIIGYRIKVIATGETVPIFEDFVDSSVGYYMVTGLEPGIDYEISVITLINGGESAPTTLIQQTAVPPPTDLRFTNVGPDTIRVTWTPPASVELSSFLVRFSPMKNKDDVAELSISPSDSAVVLTNLLPGTVYLVKVYSVYEQHESKPLSGEQKTGLDSPTGLDFSEITAHSFTVHWIAPRSPISGYKIRHHLEQGGSRPKEVRVAPSRNSITLTDLIPGSEYVVSVSAFYGKEESLPLIGQQSTVSDVPRDLQVTATGPTSLVVSWDAPAVTVRYYRITYGETGGSGPAQEFTVPGHVSTAPISDLKPGVDYTITVYAVTGRGDSPASSKPVSVNYQIEVDTPSQLQVTDVQDNSLTVKWQPSSSPVTGYRVTAAPKDGHSPTKTRVLSAEQTEVTIDELQPTAEYVVSVYAQGPNGESQPLVEKVVTNVDPPKGLAFTAVDVDSIKIAWESPQGQVTGYRVTYSTPEDGIQELFPAPEGEDETAELHGLRPGSEYTVYIVALHNDLESLPLIGTQTTAIPPPTNLKFIQVAPTSLTLTWTAPNVKLTGYRVRINPKEKTGPMKEINLAPDSTSAVISGLMVATKYEVNIYALKDSLTSRPTQGVVNTLENVSAPRRARVTDVTERTITITWRTKTEAITGFQIEAVPAGGQNPIQRTISPDVRTYTVTGLQPGTDYKIFIYALNENARSSPAELDASTAIDAPSNLRFLTTTSNSLLISWQPPRAKITGYMIKYEQVGGPTRELIPRPRPGITEATITDLEPGTEYIIYIIAVKNNQKSEPLTGRKKTDELPRLVTPPHSGKKMTEILDVPSVLENVPYLTNNPYDNGNGIPIPGSSGQQPILSNPGQQVIVEEFASRTSPTPATPDREEQPVVLIHHPFQVPDVDKPSYHHTLLSKDNYTRPQEALSRTTISWTPYTDSSEYIISCQPVSAEEEPIEIKVPGEYSSATLMGLTRGATYNIIVEAVENRRRHRVLEETVTVGNDVSERLSPPTDDVCYDTFTGSFYSIGDEWERLSDTGFKLWCQCLGLGSGHFRCDSSKWCHDNGMNYKIGEKWDRHGENGQVMSCTCLGNGKGEFKCDPHEATCYDDGKMYHVGEQWQKEYLGAICSCTCYGGQQGWRCDNCRRPGASEGSPSQTYLQREQQSPNTNIHCPIECFYPLHLQSDAQNQRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR99</accession>
    <name>A0A6I9XR99_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger protein 385B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZNF385B</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916607.1">
        <property type="nucleotide sequence ID" value="XM_014061132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR99"/>
    <dbReference type="GeneID" id="106544755"/>
    <dbReference type="KEGG" id="tsr:106544755"/>
    <dbReference type="CTD" id="151126"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000121">
        <property type="entry name" value="zinc finger protein 385B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000983">
        <property type="entry name" value="zinc finger protein 385B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000293">
        <property type="entry name" value="zinc finger protein 385B isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051845">
        <property type="entry name" value="Znf385"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF8">
        <property type="entry name" value="ZINC FINGER PROTEIN 385B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013916607.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="37303" checksum="9BC41E47A398160A" modified="2020-10-07" version="1">MVQMDPVQKAVINHTFGVSLPLKKKQVISCNVCQLRFNSDSQAEAHYKGSKHAKKVKALEATKTRPKASPSKNSAMANMNGSATPVPANISNKSDRAILKANDSSQLSRAETITFIPESASVAAAAAVATATITTPLWPVSSKSTNGTTIAISESEEEKAKKLLYCSLCKVAVNSLSQLEAHNTGSKHKTMVEARNGAGPIKSYPRPGSRLKTHGATIGSGLQNKTFHCEICDVHVNSEIQLKQHISSRRHKDKVAGKPTKPKYSPYNKLQRNPNILAAKLAFQKDILKPLAPAFLSSPLAAAAAAVSTALTLPPRPSAALFQAPAIPTALLRAGHGPLRATPASILFAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRP5</accession>
    <name>A0A6I9XRP5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106542227</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013913368.1">
        <property type="nucleotide sequence ID" value="XM_014057893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRP5"/>
    <dbReference type="GeneID" id="106542227"/>
    <dbReference type="KEGG" id="tsr:106542227"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase C-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013913368.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="13011" checksum="5BA17F21F53BAB98" modified="2020-10-07" version="1">MMLTALYCQKAGLTFVSVHDCFWTHAATVDLMNKICREQFVALHSQPILEDLSKFMLEKYCSNTTIPEGELTKKNQRAVQARIQELKDLLPKIPKKGNFKLKKVKRSIYFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XST0</accession>
    <name>A0A6I9XST0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">DAP3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917142.1">
        <property type="nucleotide sequence ID" value="XM_014061667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XST0"/>
    <dbReference type="GeneID" id="106545187"/>
    <dbReference type="KEGG" id="tsr:106545187"/>
    <dbReference type="CTD" id="7818"/>
    <dbReference type="OrthoDB" id="274828at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008092">
        <property type="entry name" value="Ribosomal_mS29_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01716">
        <property type="entry name" value="DEATHASSOCP3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917142.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19053" checksum="E5E6011384F2ABCD" modified="2020-10-07" version="1">MAWVKYASDTVGVILKELKQQSGQGSFRLLVAVDGINSLWGKTALKHNKQEVTVEELTLVHNLKKMVKNDWAGGAIVATLSQTGAPFAPRPLYLPHELLGRDGFAALDPFVPIEVRNYTDMEFEACYQYYLERKWLQHEKANTKEGRLELRFLSGRNPGLFERISAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT01</accession>
    <name>A0A6I9XT01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">N-alpha-acetyltransferase 30</fullName>
            <ecNumber evidence="13">2.3.1.256</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">N-acetyltransferase 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">N-acetyltransferase MAK3 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">NatC catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">NAA30</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-methionyl-L-isoleucyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-isoleucyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50524"/>
            <dbReference type="Rhea" id="RHEA-COMP:12713"/>
            <dbReference type="Rhea" id="RHEA-COMP:12714"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133379"/>
            <dbReference type="ChEBI" id="CHEBI:133380"/>
            <dbReference type="EC" id="2.3.1.256"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-methionyl-L-leucyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50520"/>
            <dbReference type="Rhea" id="RHEA-COMP:12711"/>
            <dbReference type="Rhea" id="RHEA-COMP:12712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133377"/>
            <dbReference type="ChEBI" id="CHEBI:133378"/>
            <dbReference type="EC" id="2.3.1.256"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-methionyl-L-phenylalanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50528"/>
            <dbReference type="Rhea" id="RHEA-COMP:12715"/>
            <dbReference type="Rhea" id="RHEA-COMP:12716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133382"/>
            <dbReference type="ChEBI" id="CHEBI:133383"/>
            <dbReference type="EC" id="2.3.1.256"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-terminal L-methionyl-L-tryptophyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-tryptophyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50560"/>
            <dbReference type="Rhea" id="RHEA-COMP:12724"/>
            <dbReference type="Rhea" id="RHEA-COMP:12725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133386"/>
            <dbReference type="ChEBI" id="CHEBI:133387"/>
            <dbReference type="EC" id="2.3.1.256"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-methionyl-L-tyrosyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-tyrosyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50532"/>
            <dbReference type="Rhea" id="RHEA-COMP:12717"/>
            <dbReference type="Rhea" id="RHEA-COMP:12718"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133384"/>
            <dbReference type="ChEBI" id="CHEBI:133385"/>
            <dbReference type="EC" id="2.3.1.256"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetyltransferase family. MAK3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.256" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013919199.1">
        <property type="nucleotide sequence ID" value="XM_014063724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT01"/>
    <dbReference type="GeneID" id="106546767"/>
    <dbReference type="KEGG" id="tsr:106546767"/>
    <dbReference type="CTD" id="122830"/>
    <dbReference type="OrthoDB" id="249099at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000010">
        <property type="entry name" value="Putative N-alpha-acetyltransferase 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044542">
        <property type="entry name" value="NAA30-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="19">
        <location>
            <begin position="252"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="103"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013919199.1"/>
        </source>
    </evidence>
    <sequence length="403" mass="45550" checksum="88E8D6D4D5A1E083" modified="2020-10-07" version="1">MAEVPPGPSSLLASPPGAAAALLSPFPGTAEEEEEEEEDEEKGGRRFCRREEEEEEAEEEGMREGSLPRRRVCQPHRHYHQLNGLIVGSDLRHLRSSLKDKVLSHSNENQCPPNSKKNSKATISTGPGKTMGHGQEPQHPCQGGERETSPSSLSNHHQVPCPQARTAQSQQQHHHHHHHHASAPSAGDRNGLVGGGELEEGEEDQELEREEESLLLLSRSLASTCSLQKCSMGSHTDTSWPPQQVKEEKGDLTIRYVRYESELQMPDIMRLITKDLSEPYSIYTYRYFIHNWPQLCFLAMVGDECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRKGIGTNLVKKAIYAMVEGDCDEVVLETEITNKSALKLYENLGFVRDKRLFRYYLNGVDALRLKLWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUH1</accession>
    <name>A0A6I9XUH1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Polycomb complex protein BMI-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106545612</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917722.1">
        <property type="nucleotide sequence ID" value="XM_014062247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUH1"/>
    <dbReference type="GeneID" id="106545612"/>
    <dbReference type="KEGG" id="tsr:106545612"/>
    <dbReference type="OrthoDB" id="1305878at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000106">
        <property type="entry name" value="Polycomb complex protein BMI-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000122">
        <property type="entry name" value="polycomb group RING finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825:SF21">
        <property type="entry name" value="POLYCOMB COMPLEX PROTEIN BMI-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING, POLYCOMB GROUP COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="224"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="255"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013917722.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="34974" checksum="8C4B37F40671A470" modified="2020-10-07" version="1">MHRTTRIKITELNPHLMCVICGGYFIDATTIIECLHSFCKTCIVHYLESSKYCPICDVQVHKTKPLLNIRSDKTLQDIVYKLVPGLTGCPSRTSLSGAEHSSCGELNLYTKGGNQCRLATTYNSICPFSRVEQKGSNEKEKANEEAKDKRYLRCPAAMTVMHLRKFLRSKMDIPNAFQVDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRQTCKRMKIGGQQRDGLHNSGDLESDSGSEKAGSPMGGAPSTSSCVLSPSTPVQSPHPQFPHISSTMNGTCGSPSSNHHASFTNRTPKVSVNSCSVTSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUT2</accession>
    <name>A0A6I9XUT2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PLIalpha-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106547281</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919869.1">
        <property type="nucleotide sequence ID" value="XM_014064394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUT2"/>
    <dbReference type="GeneID" id="106547281"/>
    <dbReference type="KEGG" id="tsr:106547281"/>
    <dbReference type="OrthoDB" id="10255512at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027002403" evidence="4">
        <location>
            <begin position="20"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="123"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013919869.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18230" checksum="04EC346402EAFE86" modified="2020-10-07" version="1">MQLLLLSSLLLLGLSLANGHDTDPEGQLLNSLSEDVGRLKEKVKELENAFFTVHRARSFGSGSERLYVTNKKVGKFGELGNICTQAGGRIPSPQLLNENEAFASVLGRHNKEAYLEVRNSATFTNWAEGEPNNTDGSKACIKTDAQGAWHSASCEEDLLVVCEFSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVA6</accession>
    <name>A0A6I9XVA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PFN3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with ACTRT3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the profilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914583.1">
        <property type="nucleotide sequence ID" value="XM_014059108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVA6"/>
    <dbReference type="GeneID" id="106543186"/>
    <dbReference type="KEGG" id="tsr:106543186"/>
    <dbReference type="CTD" id="345456"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000010">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005454">
        <property type="entry name" value="Profilin1/2/3_vertebrate"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13936">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13936:SF2">
        <property type="entry name" value="PROFILIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01639">
        <property type="entry name" value="PROFILINMAML"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914583.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="14598" checksum="3CAB3453FFC5C87F" modified="2020-10-07" version="1">MGDWKAYINTVLKDKNIEDVAIVGHSDNKCVWASKPGGLLAAISPQEVGLITGQDRKSFLQTGITIAGKKCSVIRDNLLLEKDAVMDTRTKGGDSRSICIGKSSKALIFLMGKKGVHGGALNKKVHDMIASMKAKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWX9</accession>
    <name>A0A6I9XWX9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 81</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CCDC81</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920714.1">
        <property type="nucleotide sequence ID" value="XM_014065239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWX9"/>
    <dbReference type="GeneID" id="106547953"/>
    <dbReference type="KEGG" id="tsr:106547953"/>
    <dbReference type="CTD" id="60494"/>
    <dbReference type="OrthoDB" id="125906at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026295">
        <property type="entry name" value="CCD81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040673">
        <property type="entry name" value="CCDC81_HU_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028034">
        <property type="entry name" value="HU-CCDC81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14362">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14362:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14908">
        <property type="entry name" value="HU-CCDC81_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18289">
        <property type="entry name" value="HU-CCDC81_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC81 HU" evidence="2">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC81 HU" evidence="3">
        <location>
            <begin position="103"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013920714.1"/>
        </source>
    </evidence>
    <sequence length="615" mass="70732" checksum="DF0DC8FC6453CFAE" modified="2020-10-07" version="1">MLDMIQNAITQAGKNLFITLPKLTSEDVVNIWDSVSEFVEKQLSMKKGVQIPGLGTFSFLREKLHVGSNKPIFLQRPVFLFSEKLAQIHGLKFNKIRIPGDIPIVQLNFIVLSLDSPFNRDMVEGCVRETLMSFSRSIATKQTVEFTFKGIGVLIVRDNKVKMKFYKDFLHTMDGSGTLLKALTNRPGTVDSVLSSRDASTPPFPPPNTAVFPRFEIKEMETIAEEGEKSSKEQEQLDKESSKKENMISKRFLSRQAIFPAKVTGISLTDELEKNLKPKPPPSSKQPTPIPDALKIEQEVSRIQTPAPRSQTSSPICDEHGRAGQEMCYLCMQRAQRNIPVYLGEEKRRKEKIEDCILAQYQAIKDHEALQKELVLGLETREQNQKVASYNLGVAEAMRKQKNEKLAEPFRSYIFEKRPSSSTPHEKQEEYAQHLDKQLESRKAREAKQKQEQDFIDRLQQVQLAEELAAQRAKYLREKLEENQTYKTALDTQIKLRPAPLPEFVPDSTEIIFGKNDMNEEKMEERRKRAREYSKYQLQAVADRKRTAILNELVDQRRAADMFQRAKKQAGDKLMLLDQCARYRRCYQCKKGLNKFGESKIWTDSKYIPGSRLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX32</accession>
    <name>A0A6I9XX32_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transforming growth factor-beta-induced protein ig-h3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106546382</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in cell adhesion. May play a role in cell-collagen interactions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to type I, II, and IV collagens.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918722.1">
        <property type="nucleotide sequence ID" value="XM_014063247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XX32"/>
    <dbReference type="GeneID" id="106546382"/>
    <dbReference type="KEGG" id="tsr:106546382"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000002">
        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000007">
        <property type="entry name" value="Transforming growth factor-beta-induced protein ig-h3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF82">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA-INDUCED PROTEIN IG-H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EMI" evidence="8">
        <location>
            <begin position="90"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="7">
        <location>
            <begin position="148"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="7">
        <location>
            <begin position="308"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="7">
        <location>
            <begin position="432"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918722.1"/>
        </source>
    </evidence>
    <sequence length="613" mass="67974" checksum="C5A613DB6D27C70B" modified="2020-10-07" version="1">MADPTMRLLTEAHQSDIVTLRKQLLGVLVSMSWRVDNEETLESPSSNRRWHEADVARPCICLFLMYSQDMFECQAVNGEMHNNISPLICSPNVCAVLKLIGTNKKYMPNCKLWYQKKICGKSTVVAYECCPGYEEVPGEKGCPAALPLANLYETLGVVGATTTQIYSASSNLSAEITGPGSYTIFAPTNEAWASLPAEVLDSLVSNVNIELLNALRYHMVNKRILTDELRHGLSLPSMYQNIDIKIHHYPNGFVFGKGGLVNNFCINKDNHRQHLDLSYILLKHTGDWKPGESAFRTSLLSYKLGKKAAKTLLELGKESDVSTSIDLFRQVGLGSHLTGNERLTVLAPINSFYKDHSPSPNRNLLQNHIIKEQLSSKYLFHGQKLETLGGKELRVFVYRNNLCIENACIAAHDKRGRFGTVFAMDKMLSPPMGTVMDVLKADDRFSTLVAAIQSAGLTETLNRPRSFTVFAPTNEAFQSMPQRELNRLMGNAQELANILKYHIGDEILVSGAVGAAVRIKSLQGDKLEVSSRNHIININKMPVAEADIMATNGVIYAVNSILQPPASRHNERGDEPADPSLEIFKHASALSKISLMDARLAPAYSRLLARMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY46</accession>
    <name>A0A6I9XY46_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lipoyl amidotransferase LIPT1, mitochondrial</fullName>
            <ecNumber evidence="11">2.3.1.200</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Lipoate biosynthesis protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Lipoate-protein ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Lipoyl ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Lipoyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">LIPT1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lipoyl amidotransferase that catalyzes the transfer of lipoyl moieties from lipoyl-protein H of the glycine cleavage system (lipoyl-GCSH) to E2 subunits of the pyruvate dehydrogenase complex (PDCE2). Unable to catalyze the transfer of octanoyl from octanoyl-GCSH to PDCE2. In vitro, it is also able to catalyze the transfer of the lipoyl group from lipoyl-AMP to the specific lysine residue of lipoyl domains of lipoate-dependent enzymes but this reaction may not be physiologically relevant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-lipoyl-5'-AMP + L-lysyl-[lipoyl-carrier protein] = N(6)-[(R)-lipoyl]-L-lysyl-[lipoyl-carrier protein] + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20473"/>
            <dbReference type="Rhea" id="RHEA-COMP:10500"/>
            <dbReference type="Rhea" id="RHEA-COMP:10502"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:83091"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + L-lysyl-[lipoyl-carrier protein] = L-lysyl-[glycine-cleavage complex H protein] + N(6)-[(R)-lipoyl]-L-lysyl-[lipoyl-carrier protein]</text>
            <dbReference type="Rhea" id="RHEA:16413"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10500"/>
            <dbReference type="Rhea" id="RHEA-COMP:10501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10502"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="EC" id="2.3.1.200"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16414"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LplA family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.200" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013915533.1">
        <property type="nucleotide sequence ID" value="XM_014060058.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915534.1">
        <property type="nucleotide sequence ID" value="XM_014060059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY46"/>
    <dbReference type="GeneID" id="106543952"/>
    <dbReference type="KEGG" id="tsr:106543952"/>
    <dbReference type="CTD" id="51601"/>
    <dbReference type="OrthoDB" id="201621at2759"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00595"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017118">
        <property type="term" value="F:lipoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.50:FF:000005">
        <property type="entry name" value="Lipoyltransferase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000045">
        <property type="entry name" value="lipoyltransferase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004562">
        <property type="entry name" value="LipoylTrfase_LipoateP_Ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00545">
        <property type="entry name" value="lipoyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561">
        <property type="entry name" value="LIPOATE-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561:SF3">
        <property type="entry name" value="LIPOYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="17">
        <location>
            <begin position="57"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013915533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013915534.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="42285" checksum="88F73552D2550F1F" modified="2020-10-07" version="1">MIIQFSWKKCFPLYCVLKVPAAGFKSTKERGLILQSVSQDVYQNLALEDWIHDNMDLEKRHVLLLWRNSSAVVIGRHQNPWQECNLGIMRQKGIKLARRRSGGGAVYHDKGNINLTFFTDRKKYERIENLKLVVKALKSLRPQLDVHATERYDLLLNKTFKISGTAAKLGRTAAYHHCTLLYSTDKLVLTSALKTPNSGIQSNATPSVPALVRNLYEEDPTLTCEMLMEAIAGEYASHHHIENHIHVINPADETEFPGINNTAIELKAWNWLYGKTPKFKISTSFNIGHEESNVEIQISMDIKNGNIETCQIDVPQNWLPLDVCVELRNSLIGNRFCPNENTAVTTALLRMWANSTLHSKLHILCEKIITLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZS0</accession>
    <name>A0A6I9XZS0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Osteoclast-stimulating factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">OSTF1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910010.1">
        <property type="nucleotide sequence ID" value="XM_014054535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZS0"/>
    <dbReference type="GeneID" id="106539686"/>
    <dbReference type="KEGG" id="tsr:106539686"/>
    <dbReference type="CTD" id="26578"/>
    <dbReference type="OrthoDB" id="207120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11772">
        <property type="entry name" value="SH3_OSTF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000158">
        <property type="entry name" value="Osteoclast-stimulating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24155">
        <property type="entry name" value="OSTEOCLAST-STIMULATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24155:SF10">
        <property type="entry name" value="OSTEOCLAST-STIMULATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="12"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013910010.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="21863" checksum="72056362E33D86F0" modified="2020-10-07" version="1">MSKPPPKPAKPGQVKVFRALYTFEPRTPDELYFEEGDIIYISDMSDTNWWKGTCKGRTGLIPSNYVAEQAESIDNPLHEAAKRGNLSWLRECLDNKVGVNGLDKAGSTALYWASHGGHKDIVEVLLTQPKVELNQQNKLGDTALHAAAWKGYEDIVEILLGKGARTDLRNNEKKLALDMATNAACASLLKKKQGQGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0R5</accession>
    <name>A0A6I9Y0R5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Short-chain dehydrogenase/reductase family 42E member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">SDR42E1</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916547.1">
        <property type="nucleotide sequence ID" value="XM_014061072.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916548.1">
        <property type="nucleotide sequence ID" value="XM_014061073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0R5"/>
    <dbReference type="GeneID" id="106544719"/>
    <dbReference type="KEGG" id="tsr:106544719"/>
    <dbReference type="CTD" id="93517"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000138">
        <property type="entry name" value="Short-chain dehydrogenase/reductase family 42E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013916547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916548.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="43583" checksum="37F455952E013310" modified="2020-10-07" version="1">MERGNPSKEAVLITGGSGYFGFRLASALAKSQVDVILFDVSKPTQEMPKGVTFVQGDICAIHEVEEAFQGVSCVFHVASYGMSGREQLNPKLIEEVNVKGTDNIIESCRRVGVPKLIYTSTYNVVFGGQVIENGDESWPYLPLHLHPDHYSRTKALAEMKVLEANGTVLRDGRGILRTCAIRPAGIYGPGEQRHLPRIVNYVEQGWFRCVYGNPSSLVDFVHVDNLVQAHILAAEALGATKNHTAAGQAYFISDGRPVNNFEFFRPLVEGLGYTYPSLRLPLPLVYFFAFVTELVHFAVGRLYNFQPLLTRTEVYKTGVTHFFSLAKARRELDYKPQHYSLREVVEWFRNHGHGQKFSKSSEKHFIRNAALVFLAAVMVLWWSWGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y133</accession>
    <name>A0A6I9Y133_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Immediate early response gene 5 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">IER5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IER family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920592.1">
        <property type="nucleotide sequence ID" value="XM_014065117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y133"/>
    <dbReference type="GeneID" id="106547852"/>
    <dbReference type="KEGG" id="tsr:106547852"/>
    <dbReference type="CTD" id="51278"/>
    <dbReference type="OrthoDB" id="6358394at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008653">
        <property type="entry name" value="IER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15895">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05760">
        <property type="entry name" value="IER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920592.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="28366" checksum="4D822FDAC75FA847" modified="2020-10-07" version="1">MECKLEAHRIVSISLGKMYSARGQRGGVKLHKNLLVSLVLRSARQVYLSELGCSPSPPLAAAAAAPPSAAAAPNPLREGGEEGGRPPLPFFSPPRAFLPPARAMLPCDPRTSPWCRKLPSSGWEVQRLPRCCGSCCGSSFASEAGKRPAEAAPALDCSRKRNAALHPEETSPGTSSPLKKSRREKEEEEDDMETGNVANLISIFGSSFSGLLSKKSCGGNNPRRRRQEKEEEEEAGGGEAEPGQICCDESVLRNLNPWSTAIVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1I4</accession>
    <name>A0A6I9Y1I4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RPA-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RPAIN</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916843.1">
        <property type="nucleotide sequence ID" value="XM_014061368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1I4"/>
    <dbReference type="GeneID" id="106544948"/>
    <dbReference type="KEGG" id="tsr:106544948"/>
    <dbReference type="CTD" id="84268"/>
    <dbReference type="OrthoDB" id="435311at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028156">
        <property type="entry name" value="RIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028159">
        <property type="entry name" value="RPA_interact_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028155">
        <property type="entry name" value="RPA_interact_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028158">
        <property type="entry name" value="RPA_interact_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31742:SF1">
        <property type="entry name" value="RPA-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31742">
        <property type="entry name" value="RPA-INTERACTING PROTEIN RPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14768">
        <property type="entry name" value="RPA_interact_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14767">
        <property type="entry name" value="RPA_interact_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14766">
        <property type="entry name" value="RPA_interact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RPA-interacting protein N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="RPA-interacting protein central" evidence="7">
        <location>
            <begin position="59"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="RPA-interacting protein C-terminal" evidence="8">
        <location>
            <begin position="139"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013916843.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="25247" checksum="95A1BED2B61656C1" modified="2020-10-07" version="1">MAGLVPRHQALYKGFRTPPWKETFRQRCVDRLKSSRAKLLERYRQVGENGASRPNKTLLVQEVMEVEWKALHSVDSQLPSLRKHDSSQMLEELAVLDEIQQELILQEQLAIEEYEQSLKFEEECLKAMLEGLDAERHIICPVCRRNNLSVMSNMVACQCGLCINSLGMTEEKLQLLLEEGLMEHSQNCQHCPEFTVTNMMEEANLLMSCQACDSWTVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2H5</accession>
    <name>A0A6I9Y2H5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zona pellucida-like domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cupulin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZPLD1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Glycoprotein which is a component of the gelatinous extracellular matrix in the cupulae of the vestibular organ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911015.1">
        <property type="nucleotide sequence ID" value="XM_014055540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2H5"/>
    <dbReference type="GeneID" id="106540442"/>
    <dbReference type="KEGG" id="tsr:106540442"/>
    <dbReference type="CTD" id="131368"/>
    <dbReference type="OrthoDB" id="9274484at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF24">
        <property type="entry name" value="ZONA PELLUCIDA-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026647022" description="Zona pellucida-like domain-containing protein 1" evidence="15">
        <location>
            <begin position="20"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="16">
        <location>
            <begin position="43"/>
            <end position="320"/>
        </location>
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    <sequence length="416" mass="45745" checksum="75FDBBBF6FD61A38" modified="2020-10-07" version="1">MERIWLPLLLSVKLLSVTAQFNGYNCDANLHSRFPAEQDINVYCGVQAISMKINFCTVLFSGYSETDLALNGKHGDSHCRGFINNNTFPAVVIFIINLSTLESCGSSLMVTSVPGINVYGNASTVQIGNISGYIDTPDPPTIISYLPGLLYKFSCSYPLEYLVNNTQLASSSAAISVRENNGTFISTLNLLLYNDSTYSQQLIIPNTGLPLKTKVFAAVRATNLDGRWNVLMDYCYTTPSGNPNDNLRYDLFFSCDKDPQTTIIENGKSQMGRFSFEVFRFVKHKNQKMSAVFLHCITKLCRADDCPFLVPACSNRKKRDVMKQTTWTPQSISGSAVISAGPIITRTEEIPTNNSQLGSPNGFSFQLNSVTSALISGIVILGIISTISCVLSLLLFYRKGHSSNLILSGVRNPVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2X4</accession>
    <name>A0A6I9Y2X4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Xin actin-binding repeat-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">XIRP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Xin repeats bind F-actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Xin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921026.1">
        <property type="nucleotide sequence ID" value="XM_014065551.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106548214"/>
    <dbReference type="KEGG" id="tsr:106548214"/>
    <dbReference type="CTD" id="129446"/>
    <dbReference type="OrthoDB" id="6129702at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012510">
        <property type="entry name" value="Actin-binding_Xin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030072">
        <property type="entry name" value="XIRP1/XIRP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22591">
        <property type="entry name" value="XIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22591:SF1">
        <property type="entry name" value="XIN ACTIN-BINDING REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08043">
        <property type="entry name" value="Xin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51389">
        <property type="entry name" value="XIN"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        </source>
    </evidence>
    <sequence length="3338" mass="379556" checksum="4CDDDA25BFC38211" modified="2020-10-07" version="1">MKSILLKERGEFQWSWSPLDCIFRGLGTLSHKGKQERIFNGGLPRSSNTGSTGSKKSGEGLSEGIKESDHGPLEIQEAISLKERMALYQAAASKVDISNSSANALEESEACRIPGGLASVKNQFEKGEAQYQYRQKSVQKRTSQSQTMMSSSRTETSINEATSKGVQLETFQSEKVSHQGQASMEAKKMSTTTQNIDRTGILFSLLKIENGYQEMVWPSANVHSSAEATSTTRGQGTYMARKTEHTSAASTLDCNKFLKYGSTEEFPPPPSPDLLQVPSEMTEFSQSPEPPLSPSKQVLPKDLYSRQRNLYELNRLYKHIHPELRKNLEKDYFKDISDIVCGSNTEIERSIGGEVLQAKQVFENSENSPQKSMSPERENLEWDEILKGEVQSMKWIFENQPLDSIKDESSDQDNVKTIAGQEIIAGGDVKYTTWMFETQPIDALSANFSDDAGAADRIPDLARGDVRTGTWMFETQPLDAMNKIHHEQEEELNENSVEEITGGDVKTVKYMFETQNLDSLGQLYSVDEAKLLQLRSELKEIKGDVKRSIRHFETLPMYVIRNDLGQMLEIKTVHREDLEKGDVKTVHWMFETQPLDVINKDSVDIKVVRGISMEENVKGEVSRAKWLFETQPLDTIKECEEPAAEKEVILGADVCRKCWLFETQPLDTLKENEDPIISPTVEIIGGDVNTTKHLFETLPMDLLKDSPEVEKLQKMAATEEEKGDVRHQKWIFETKPLEQIREERKEFVRTVKLEEIDKGDVSSCKHAFETCWLSKRNDSHKIHVEGVTRGAVKLNKDIFETTPLYAIQDNLGKYHQVKTIRQEEVIRGDVRNCRWLFETRPIDQFDESIEKMEIIKGITSKEVQSGDVKTAKWLFETQPLDAIKYFSNVEEEESEQQTEVTEVVKGDVRMCKWLFETQPMESLYEKETTVTNTDEVQRGDVKTCTLLFETQSLDAIGVESETAKLHMVDQEDIQGSDVRTACFLFETEKLENIQGGEEDNELKRIVEIDIQPGDVSTMKYKFETQPLDSLSIDSEEVLHKIKTISREDIQKGDVLRCCWLFENRSLGEISEDKEERTSAQTVTDVQDGHVKNGCFIFETFSLDQIKDEDGMEENTKKTINHEEITKGDVKSYKMLFETQPLYAIQDKEGYYHEVTTVKKEEVLHGDVRGTRWLFETKPLGSINESENVYVIKSVTQEDIQKGNVSSVRYRFETQSLDTISDEEKLFVPTVNSVQGGDVKANKQLFESEETQRDKYVRTVNVSEIQQGNVKTCSWLFETHTIDEIRGEDSEYKDIQTVTREEIQEGDVQHAVWLFENQPLDAIKENDESNTEIDKEEIPQADVKTTTWLFETTPFHEFNESRIEKEEIVGKSVKETLKELYSQKVVETHGIILEADEIGDVRMAKYRLMNQEAPEIQKEEIIKGDLANIMINLLSKPSTLERESKVNEDEKGNVDLTKKQLMNSSTAMQVEKEEVMRGDIQQAIKNLFNQDRFVKRGILIQESERGDVNMTIYSLFHKKESNKTEQDEVIGGDVKRTIHSLLSSVTNHEIAERPKIDDSERGNVQFFTTCIEAGALDYLKLLQSETNEAFARKNQDEEEEIVGGDVEGTKLLLKKKKSQIQRTVNEADIIPGDVCNALKIFTTEPQDTSCHVDKEEIIKGDLKTTLNSLSQAINQTTVTEKEEIAKADIHAILKSLQESVHQPRETEKPEVVPGDIKQTIESLEKTKQTKNEVWREELVQRDLESTLRPFKAVQQSFLETDKEVVRGNTQTAVESLLDTSEETKLRQYQRFTEAQAKQSKERLLKPSQQLAQNKVNPTESLKNNDTFLQKEMMSQRKSFLSEDAKAIHLDPRWNTKSYEMERRNMKALPKSQHKVTEKADKSSVDKVTAKRHRDQYVIDQSTLCNNVEKNERAEESEASRILMKGHATNEMQSAKQMTGKKQSIFHEYKNEKYVGVRSQGETKVKLAQPTCDRQSSNYRGTEQLINQSTGLISKAAGHLETKEVQQDRKQSQHSKEVCRGKKNNVVASQAQVSMASEQNVKTNQKVRVTQEMHRQFKEAERKQKCSEKSILKYSGKYNVEGAEEDLRVQVKNLGNPYKNPKGSDRSEIDSPPPPPPPPPPPLPLASSEVDFPLPPPPPHLLMPCDRRRLPSPPPPSNEEVKSVHESFPPPPPTAEEKCDNKLTSHRPPPPLPQARERFFKSLEKTVQSQSGTSYNSKHLQAELMKELEASQGKTSRKMQPRVIQMHKEIDKTNTVEERAESSMFRNTVSHERKEKTVCTKNEDVKRAASAVDTINLEVSLSNKTFPHFRSPSLPAEATQASPKPYVRKFKTPLMIAEEKYKLQKEEMEQKQSKDVCQLQVRRNSESWGRLAQAESEGGLPGPKSYKQDEITPEGSLPSEVPETPAEPECQRKPQVINSEADDPQSLPKQFTAEQSQKVSKRSAEEHIRKEAVHESQRLTKQSASSEKVQTHKEHAMSQKEQHQKEEQLHVPNNKTAYPSFKVKTIQVSACDHTLPKGHRDSMIYKKQEGSSGVVRQQVQEKQKESISKKSAAGNCMKVDGKIHMEKTLFKHAEGRQEIGDKESSETKQRLVQRKETEKDKMKQENNSLAMEGKQSQVIAHLKEEKGIIQRKEQMTSSKSEKMVKQKIINIHNQFQTVHSKPESTFVLEANVQNKNLSQEKNNLQGQGEYTWEITSEQKKSPHKTPQPKKEPMQEKTLPSSSPKGSLQKSEKDSVDILELLRKREEIQQLLSRMKEFEMEPSKNGMKTFQVFLSVIPEWLVGQEKKQDLSYIAQEDSVEKMREELLVIKDQASKILHSCEEAIQAAMVSTNSLKCKKNVHCARESSQKIAKVTVGSSRKDSQMAKEMSKDTMAHQEVKQKVSANRFSETTTSSPSLRMRAPSPTFITIESRCVESPLRDLLSPIQRESTPVPPSPPPRSTTPTSKIQTSSARSEQLAKLKDSTAKLSQGAIPIRSITPIPIVEKRSEIVKSPASLRRQIKIDTRQLDLCKVPFPKDTCVTAETVAKVKETHEESQVNQMYVSQKGRSIPEEPRGQSVGSISITHGFEVPKTGPKGLRHRYEASDQVIHIRKEPELPERFGSDYKYKTDAMLQTFNDQTLSERKFKNKYVENGIMNDRSKEVRCRSQKDRLSQTSQESDNRKDTFQKCPGKHQRESKETNSQKQNQRVIKEHSCEPRVCFDAKSPLGCSYGMESFENTVIESRTAASTAQSTDGSQSGFGFKRAPPTYEDVVSGHILDIAAPNSPEDLLRNFQKTWQESERVFQSLGYTMSEATEAEVRSSFHEEAEFISESATSGKGNMPTLSKESLSNGVPSCRQADFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2Y2</accession>
    <name>A0A6I9Y2Y2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cystatin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CSTA</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917518.1">
        <property type="nucleotide sequence ID" value="XM_014062043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2Y2"/>
    <dbReference type="GeneID" id="106545470"/>
    <dbReference type="KEGG" id="tsr:106545470"/>
    <dbReference type="CTD" id="1475"/>
    <dbReference type="OrthoDB" id="2429551at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000001">
        <property type="entry name" value="Cystatin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF22">
        <property type="entry name" value="CYSTATIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="7">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917518.1"/>
        </source>
    </evidence>
    <sequence length="98" mass="11472" checksum="C2F147ADD6EC75A6" modified="2020-10-07" version="1">MSCGGLSEPKAATAETQQITQEIKSQLEEKESRNFNIFDAVLYKAQMVAGVNYFIKIHVGNEEYYHVRVYKRLPHENKPIELTNYQSKKEKHDDLTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3B3</accession>
    <name>A0A6I9Y3B3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger and BTB domain-containing protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZBTB11</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921281.1">
        <property type="nucleotide sequence ID" value="XM_014065806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3B3"/>
    <dbReference type="GeneID" id="106548439"/>
    <dbReference type="KEGG" id="tsr:106548439"/>
    <dbReference type="CTD" id="27107"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18202">
        <property type="entry name" value="BTB_POZ_ZBTB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001897">
        <property type="entry name" value="Zinc finger and BTB domain containing 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000002">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001072">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001086">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000070">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048060">
        <property type="entry name" value="ZBTB11_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF41">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="214"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="572"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="600"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="650"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="678"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="706"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="734"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="765"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="793"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="143"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="440"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013921281.1"/>
        </source>
    </evidence>
    <sequence length="822" mass="93387" checksum="9D09CB84335365D8" modified="2020-10-07" version="1">MSSEESYLAILRYLTNEREPYAPGTEGNVKRKIRKAAACYVVRGGTLFYQRRQRDQQRFAELEVVLQAERRGRLIRAAHLETDGSHRTRLQTWQRLSRRYWWRGILKQVKDYIKECSKCQEKLDRSRSLSDPSEMLEELGLDAVSHEDSNETDDEINNSTTLPAASPKPVKKKPIAKHELVFVDSKGLVKQSSPKHSQSVLNQLNQQRLSSQFCDVTLLIEGEVYKAHKSILAANSEYFRELFIEKGAVSSHEAVVDLSGFRKASFLPLLEFAYTSELVFDFCNMAAVAELAQHLLMTEVLAICENVHKQMEDKQITVYQKGDVQVVEVAQTISEQNEIEANPVASTEQAIATEDPGATVNGTSSNTEAEEMTVCETALEIVSEPPAKNLPELPVDTPALETTENEEKAPVVECSTNQTITVVQTEPSVAEQVIISTQAENEADQNMNGDPNVNSELNVSSEANAEVDVEDSQEGLKRKRGRPCKMQDIPESQPETTALSQDDTYKSKLRQRSVSEGGYIRLHKGTEKKLQNRKPGPKSAIQQVAMKLVQRGKKMKQPKREIIEHSEAEAQHKCSECGMIFQRRYALIMHTLKHERTKNYKCPLCKKEFQYGASLRAHLVRHTQKNEVTVATANIEESGAATKGRTKREFICDICGRSLPKLYSLRIHMLKHTGVKPHACKICGKSFTYKHGLKMHLVLHEAQKQFKCESCDKSFVTKRSLQEHVSIHTGESKYFCSHCGKSFHRASGLSKHLKKHQPKPEIRGYQCNQCEKSFYEPRDLRQHMNKHLGVKPFQCQFCGKCYSWKKDWYSHVKSHSVTDPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3Q5</accession>
    <name>A0A6I9Y3Q5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-amino-acid oxidase</fullName>
            <ecNumber evidence="5">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106545932</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="17">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013918133.1">
        <property type="nucleotide sequence ID" value="XM_014062658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3Q5"/>
    <dbReference type="GeneID" id="106545932"/>
    <dbReference type="KEGG" id="tsr:106545932"/>
    <dbReference type="OrthoDB" id="333024at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050711">
        <property type="entry name" value="ET-N_metabolism_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11938">
        <property type="entry name" value="FAD NADPH DEHYDROGENASE/OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11938:SF91">
        <property type="entry name" value="NADPH:ADRENODOXIN OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013918133.1"/>
        </source>
    </evidence>
    <sequence length="216" mass="24300" checksum="B4B76C9DDA60D7EE" modified="2020-10-07" version="1">MVQWLAGEQKCKNLKPDLNSETAVILGHGNVALDIARILLSPLEILRKTDITEESFSALASSKVRRVYLIGRRGPLQVAFTIKELREMINLPSVRPFLDPADFRDLGDAIKDIPRPKKRLMELMTKTALEEPPEEVGGRWASASREWRLKFLRSPVEVLPTMDGKRARAIQLAVNRLEVRSQTFSGPSALLTFALSLIYPFKVGNNLHTDIFLAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3T0</accession>
    <name>A0A6I9Y3T0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106540996</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911790.1">
        <property type="nucleotide sequence ID" value="XM_014056315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3T0"/>
    <dbReference type="GeneID" id="106540996"/>
    <dbReference type="KEGG" id="tsr:106540996"/>
    <dbReference type="OrthoDB" id="435430at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903527">
        <property type="term" value="P:positive regulation of membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13251">
        <property type="entry name" value="PH_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11965">
        <property type="entry name" value="SH3_ASAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000001">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000006">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038016">
        <property type="entry name" value="ASAP1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF2">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="11"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="751"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="408"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="476"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="627"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="507"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="693"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013911790.1"/>
        </source>
    </evidence>
    <sequence length="813" mass="89385" checksum="E246A2F2E5CA9E65" modified="2020-10-07" version="1">MHQLQGNKEYGSEKKGYILKKSDGIRKVWQRRKCTVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLITHNRTYHFQAEDEQDYVAWISVLTNSKEEALNMAFRGEQSTGENSLEDLTKAIIDDVQRLSGNDVCCDCGSPGTWVYINNELLRKCVFFVARSSLGHQDQRFSLPSFWAHAGVHLQGTSVPAECVLWLAKNIGNNSFNDIMEGNLPTPSPKPTPSSDMTARKEFITAKYVDHKYSRKTCASAAAKLNELLEAVRSRDLLALLQVYAEGVELMEPLLEPGQEPGETALHFAVRTADHTTLHLIDFLVQNCGNLDKQTVLGNTALHYCSIYNKPECLKLVLKGKPTLHIVNQAGETALDTAKRQKAIQCEDLLSQAKVGKLNPHVHVEYEWNLRQEEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKMAIPGLSTPREKQRLSYGAFPHQIFVSGSIDSATSPTTVDGPPLPPRNAGKGPTCPPSTLPLSTPASSGSSTLSKKKPPPPPPGHKRTLSDPPSPQPHGPQNKVQMSWGNDLGPPATKTANKFEGMSQQASTGSAKSALGPRVLPKLPQKVALRKIETIHLPSVDKSSHHSEIFQKSASLCIDLPQKPQPADLAPKPGELPPKPQLGDLPPKPQLADLPPKPQMKDLPPKPQLGETLAKSPGGEQSPRSQPPEANQKSPNMDLSPKIQSKEMVQKQASEDSGDTTPGSAETPVPLPRKINVARNKVRRVKTIYDCQADNDDELTFVEGEIIIVTGEEDQEWWIGHIEGKPERKGVFPVSFVHILSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y679</accession>
    <name>A0A6I9Y679_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Phospholipase A2</fullName>
            <ecNumber evidence="3 8">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106547743</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The N-terminal C2 domain associates with lipid membranes upon calcium binding.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="3 8"/>
    <dbReference type="RefSeq" id="XP_013920468.1">
        <property type="nucleotide sequence ID" value="XM_014064993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y679"/>
    <dbReference type="GeneID" id="106547743"/>
    <dbReference type="KEGG" id="tsr:106547743"/>
    <dbReference type="OrthoDB" id="419768at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040723">
        <property type="entry name" value="cPLA2_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF22">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18695">
        <property type="entry name" value="cPLA2_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 7 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="107"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="367"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013920468.1"/>
        </source>
    </evidence>
    <sequence length="909" mass="102977" checksum="A6861899179481C7" modified="2020-10-07" version="1">MSHYLTLADIPATRGRKFGYADNLALTVQDKNLEVLDCLLSEALKSLRKFFADWRLIPNIIKTITICHSKSQESEQAAEGSACSSASSPLLMLSSAMLGRLSTRMLPLLAVNIIKKKDMEFEVHPYYNLTVKILRARNIHGLDLLSKSDCYVALKLPTASPLIKRTPVVYNSSDPEWNETFQYRIHSAVKNILELSFYNRGILLSSLNSYDTSVAFDVGNIAPGQNLTHTFVLNSEAQPCLCIKGTINKRKIAPWIIRDNCQINLSVPGSFENQLTMFCGENLEENHEEIPFIFHVNKEVSSHLNLKLLQTIAILEEGWSEDLQVKTSLVGNAIIPVSELPLGKEVELTVPLGRDQALDLTVKVEERNHPIDMDMRIGFKLSKGEQEFLARRRSIVSHALKKVLKLRVPPWKHEVPIVAVLASGGGMRAMTSCYGSLSGLQQLGLLDILTYFSGISGSTWCMCMLYKDPYWSQKNLQNAVNSAQKIVTSGKVGAFSAEQLAYYFQELIALEKTGRKATLVDLWGLIIEYILNNKKDPTKLSDQQKAVIKGQNPYPIYAAQNVRLNIKTSEFTEWVEYTPYEFGIYKYGAFIRMEDYGSKFFMGLLLEKHEEPRICFLQGIWASAFAGNIDDMWEDAEISQLKFTESVKDAIRIADELLGSQSLDPSLKQSQGIMPGGVFTHLFSNLVKSRITTGGNFNFLHGLHLHRNYVNDQEFVAWKGTHLDAIPNQLTPQESSLHLVDGGFAINSPFPLVLQPERDVDVIFSFNYSWQAPFEVLHQAQRYCEERGIPFPHIAVTDSDKKQPKECYMFLNATDPRAPIILHFPLVNETFKKYKAPGIKRETEEEKSFGDFQLYTEDSPYSSTNLTYKPIQFDRLLQLNCYNIINNKDTITKALTMAMMRKKFKKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6F7</accession>
    <name>A0A6I9Y6F7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Neural cell adhesion molecule 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">NCAM2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913995.1">
        <property type="nucleotide sequence ID" value="XM_014058520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6F7"/>
    <dbReference type="GeneID" id="106542709"/>
    <dbReference type="KEGG" id="tsr:106542709"/>
    <dbReference type="CTD" id="4685"/>
    <dbReference type="OrthoDB" id="190835at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05870">
        <property type="entry name" value="IgI_NCAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000086">
        <property type="entry name" value="Neural cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000381">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000436">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000450">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000528">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000636">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009138">
        <property type="entry name" value="Neural_cell_adh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF231">
        <property type="entry name" value="NEURAL CELL ADHESION MOLECULE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01838">
        <property type="entry name" value="NCAMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026893606" description="Neural cell adhesion molecule 2" evidence="16">
        <location>
            <begin position="19"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="693"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="111"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="207"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="300"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="399"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="493"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="591"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013913995.1"/>
        </source>
    </evidence>
    <sequence length="759" mass="84576" checksum="8D47329F77565C9B" modified="2020-10-07" version="1">MNLPVLYLFGLFLSSGQALQVSISLSKVEVSVGVSKFFTCTVIGEPESIDWYNPQGEKIISNQRVMVQKEGVRSRLTIYNANVEDAGIYRCQATDSSGFSQEATVVLEIYQKLTFQDVSSPQEFVQGEDVQVICHVISSPAPIVSWLYQNEEVSNIADNRFAMLPNNNLQILKINKSDEGVYRCEGRVEARGEIDFRDIIVIVNVPPIITLLQKSFNATADRGEAVTLFCKATGSPEPEINWYRKGKRIEESDKYTLRGRNMELTIKDIKNVDSGPYICEARNKAGRVTNQTFMQVFVQPQIIQLKNETTFENGQATLICEAEGEPVPEITWKRAVDGITFSEGEKNPDGRIEVKGQYGRSSLHIKDVKLTDSGRYDCEAASRIGGHQKSMYLDIEYAPIFVSNQTIYYSWEGNPINISCDVLSNPAASLQWRRGKLVLPVENTIHLKTHSSENKLILEIAPTSDIDFGHYNCTATNRIGKRYQEYILALADVPSSPHGVKIVDVSQTFAKVSFNKPDSHGGVPIHHYQVDVKEVTSETWKVVLSHAVQTMVILSNLEPNTTYEIKVAAVNGKGQGEYSKIEIFQTLPVREPSPPSIHGQPGSGKSFKLSITKQDDGGAPILEYIVKYRSKDKEEQWQEKKVHGNKDFIILEHLQWTMGYEVQITAINRLGYSEPTLYEFIMPPKPNIITGTLFNGLGLGAVIGLGVAALLLILVVTDVSCFFVRQCGLLMCITRRICGKKSGSSGKSKELEEGKAAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8F8</accession>
    <name>A0A6I9Y8F8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neuropeptide B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NPB</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuropeptide B/W family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916850.1">
        <property type="nucleotide sequence ID" value="XM_014061375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8F8"/>
    <dbReference type="GeneID" id="106544952"/>
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    <dbReference type="OrthoDB" id="9942334at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013297">
        <property type="entry name" value="Neuropept_BW_pre"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28553">
        <property type="entry name" value="NEUROPEPTIDE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28553:SF1">
        <property type="entry name" value="NEUROPEPTIDE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15180">
        <property type="entry name" value="NPBW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01888">
        <property type="entry name" value="NROPEPTIDEBW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026936386" evidence="7">
        <location>
            <begin position="25"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916850.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="13725" checksum="CBFA3CCF25DA2F55" modified="2020-10-07" version="1">MSGPPILALLCLTLALLLSPPAGAWYKQAAGPSYYSVGRASGLLSGIRRSPYMRRSDDNGAADSSAEDGPAASSLTMSESRQNFGGLRNLAVCVKEVAPELQSCQLLPGVPSLIQCKADVTVSLDPTDCTIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y986</accession>
    <name>A0A6I9Y986_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell division cycle protein 16 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CDC16</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918240.1">
        <property type="nucleotide sequence ID" value="XM_014062765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y986"/>
    <dbReference type="GeneID" id="106546008"/>
    <dbReference type="KEGG" id="tsr:106546008"/>
    <dbReference type="CTD" id="8881"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="446"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="480"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="578"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918240.1"/>
        </source>
    </evidence>
    <sequence length="621" mass="71688" checksum="4CDC67917DBC14B7" modified="2020-10-07" version="1">MNLERLRKRVRQYIDQQQYQSALFWADKVASLSHEDPQDIYWLAQCLYLTSQYHRAAHALRSRKLDKLHEACQYLAARCHYAAKEYQQALDILDMEEPINKRLFEKYLKDESAVKDCSNDWEISQTSIKSSICLLRGKIYDALDNRTLATFSYKEALKLDVYCFEAFDLLTSHHMLTAEEEKELLESLPLRKQCTEEEQVLLHFLFENKLKKYNKPSETVIPESVDGLQDNLDVVVSLAERHYYNCDFKMCYKLTSVVMEKDPFHASCLPVHIGTLVELNKANELFYLSHKLVDLYPNNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLERTYGPAWIAYGHSFAVESEHDQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGDWKTSEKWFLEALEKIKAIGSEVTVDKWEPLLNNLGHVCRKLKKYEEALEYHRQALVLIPQNASTYSAIGYVQSLMGNFESAIDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGTAIKDKLRCYDFDVHTMKTLKNIISPAWGALEFDIERQTTEESNMALETPQRKTTEGPPPLDETFEIEMNESDMMLETSMSDHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA08</accession>
    <name>A0A6I9YA08_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Centrosomal protein of 68 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CEP68</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919600.1">
        <property type="nucleotide sequence ID" value="XM_014064125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA08"/>
    <dbReference type="GeneID" id="106547084"/>
    <dbReference type="KEGG" id="tsr:106547084"/>
    <dbReference type="CTD" id="23177"/>
    <dbReference type="OrthoDB" id="9448174at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF2">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013919600.1"/>
        </source>
    </evidence>
    <sequence length="661" mass="75008" checksum="BFD4F72CBDAE8E99" modified="2020-10-07" version="1">MEFLYSILLQVSPCLTIQSSDRFYKKKPKYLERKLLIDKNLNGSDMRHFSRSDLNSEKHQDGVKCSDGTKPFHNMLEFSRVLPTKAPTAMFLSPAAGSLSDRDTDCRRQALVIPYSSTPRHLPDKRSPHFSAKMSVLPRNFSSSQPLEDTGFESQELKRSRSVSATGACPSYEMPVPWYQNNLTDYEESSKLGMQKLTPARSCSVAGQMRGMSAFQADYWACAIPDFLPPSPDRQSPLWNPNKEYEDLLDYTYPLRPKYKLPKNLKYSTVHDSGIDLDSLSISPESTLKSVSMWDQEHKTRESPTIQRLSNPFLKKLECSVPVSHYRISPIGKMSLAEGGIDISGRMTPSLSPRCSEPSHSAFVTEECHKKRHGGVSRRRATSGNFLRTTSVLAFQQEWATDDEYLSLPPKLMELETLAQRLAKVTLTIKKPEFGQVQDDFPCISVNGEQLLSEVQGDSSDGNESQWELYSDSFHTCNFQEPDEMDILRDRTSKEQESESRETASTDFLEIRCPEFLSMKEKKDHHSLAQCIKVFCCQLEELIGWLQKVAEVTEQWIPPKPDVESVKASFQNYLELKKDLAKYQALTESVLQDGENLLKCMSTNSPVLQETLGLIAKHSEMLKSHPERLYESILTAMCTLDDGLKKHCDVQKTAVHKDISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB51</accession>
    <name>A0A6I9YB51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Splicing factor U2AF 65 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106548480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921332.1">
        <property type="nucleotide sequence ID" value="XM_014065857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB51"/>
    <dbReference type="GeneID" id="106548480"/>
    <dbReference type="KEGG" id="tsr:106548480"/>
    <dbReference type="OrthoDB" id="10266058at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12230">
        <property type="entry name" value="RRM1_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001887">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="144"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921332.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="28101" checksum="A7573FC1167D30CB" modified="2020-10-07" version="1">MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRERRNRDQRSVSRDRRRRRSPLSLSGPLTRSPRHEKKKKIRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBE8</accession>
    <name>A0A6I9YBE8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zonadhesin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106548588</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921456.1">
        <property type="nucleotide sequence ID" value="XM_014065981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBE8"/>
    <dbReference type="GeneID" id="106548588"/>
    <dbReference type="KEGG" id="tsr:106548588"/>
    <dbReference type="OrthoDB" id="5945029at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052749">
        <property type="entry name" value="Alpha-tectorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160">
        <property type="entry name" value="ALPHA-TECTORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160:SF8">
        <property type="entry name" value="VWFD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MAM" evidence="9">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="10">
        <location>
            <begin position="146"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="10">
        <location>
            <begin position="622"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="1019"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="1063"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="77"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="1028"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="1047"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013921456.1"/>
        </source>
    </evidence>
    <sequence length="1084" mass="117380" checksum="D2EA8F5B2B3D5D25" modified="2020-10-07" version="1">MCFSFWYHMYGVAQKMALHFYVILDDAPPFLVWSETGSKGNRWKTGEFGIAHQGRVKILLEGVRGEDFRSDVAVDDISVQGGDCPSRTTSTPGPTTTRTPPTPSQEPTPTTTEPTTPEPTPTPTEPTTAEPEPTPSPTRPTVPAPSICTASGDPHYTTFDGQVHHFMGNCTYTLAKACSNSTRGLEAFDVSTTNEHRGSNRAVSYVHSVHVAVHGSQVSLLKNRKVNVNGTRRNLPVYIGGNKIVVRLSGSYVILITDFGLSVRFDGNHYVEVAMPAEYQGLLCGLCGNFNGNPGDDNLKPDGEPAGDSTQLGNSWLVPDNSSICSSPAESCDPKLEEEIQRNSACRLIADPTGPFGQCHALVDPARFLENCIYDVCLTQGQQTSVCHGLQAYAASCANAGLCVEWRNSTLCPVSCPVNSHYSSCGTSCPSSCVKPPGLPPCSPLPTDGCFCNEGFVLSGDTCVPEADCGCVDGQGNYYQVGFPPLGREAGRTRSGPASLAADLHSKGTILHTRRSIRTDFNIYAGELCEKPPGGQCLEGCVCHQGFVLSDDQCVPLSQCGCQDKDGRYHKIGESWLTPHCSQKCRCRKGGAIKCQDFGCEPGEVCDTKKDGKLHCKPTGFGNCLVTGDPHYLTFDGLLHHFQGLHTYVLAQTRPDASDRLEPLSIEGTNRLVAGSGQPSVLKELRIRVYNHTVLFRQRRKLVVDGVATEPPARPHEGLRIQQSGRQIFLESDFGLSVSFDGLENSGILLPNSYKRSLEGLCGNFDGRFKNDFAKPDGTLVKDVQTFGESWRVPVQRGSSHLRRNRVAEEPPEEAELDVGFSPLCSEEDIKVFSGNSACGILADPKGPFRGCLAHQDPDPFLQSCVFDMCREANRSARLCPILEQYAKGCQSKSLSVADWRAASGCAVQCQPHSHYNPCMSACPPSCSDLAAPASCSSPCLEGCECESGYVLSGFDCVPFRDCGCSFLGKYYKVGDRFMADDCSQDCVCRDSSSLDCKATGCPQDFHCRIFNFTRGCYQADPCEPNSCANNGTCVLDDSPDTFHCECLENYEGLLCETRVTPEADPCQPNPCLNNGICVRHNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC21</accession>
    <name>A0A6I9YC21_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Splicing factor, suppressor of white-apricot homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Splicing factor, arginine/serine-rich 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Suppressor of white apricot protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SFSWAP</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921678.1">
        <property type="nucleotide sequence ID" value="XM_014066203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC21"/>
    <dbReference type="GeneID" id="106548753"/>
    <dbReference type="KEGG" id="tsr:106548753"/>
    <dbReference type="CTD" id="6433"/>
    <dbReference type="OrthoDB" id="5836667at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000003">
        <property type="entry name" value="Splicing factor, suppressor of white-apricot homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000005">
        <property type="entry name" value="Splicing factor, suppressor of white-apricot homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040397">
        <property type="entry name" value="SWAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13161">
        <property type="entry name" value="SPLICING FACTOR SUPPRESSOR OF WHITE APRICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13161:SF15">
        <property type="entry name" value="SPLICING FACTOR, SUPPRESSOR OF WHITE-APRICOT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SURP motif" evidence="16">
        <location>
            <begin position="4"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="16">
        <location>
            <begin position="256"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="58"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="201"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="315"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="390"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="533"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="443"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="571"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013921678.1"/>
        </source>
    </evidence>
    <sequence length="605" mass="66741" checksum="9397F37649B50C4C" modified="2020-10-07" version="1">MHAIIERTANFVCKQGVQFEIMLKAKQARNSQFDFLRFDHYLNPYYKFIQKAMKEGRYAASSEKKKEEKKNAMEDGDEDDDDDDDDNEGNYLHPSLFASKKRSRLEELMKPLKVVDPDHPLAALVRKAQAENASAAQAGEGTAAPSSQMEYSSDPTMAAMYYSYYMLPDGTYCFAPPPPGIDMATYYSALPAGVTVSSTTGVATITAPPPPGTTPPPSSSEASEDTSGALPGTSTSASSIPPVVAVIPPPPDIQPVIDKLAEYVARNGIKFETSVRAKNDPRFEFLQPWHQYNAYYEFKKQFFLQKEASDSSKVASLSEDVSTDTPSVAPGETQFPAEQSPEDADSAVEGNNAKDAAAIKISNDGKLIKVSFAPISFAIRAKENDMLPLEKNRVKLDDDSDEEEEEGKEGQENASSTSNSAALLASSSSAPEEKKPQLTQEELEAKQAKQKLEDRLAAAAREKLAQASKESKEKQLQAERKRKAALFLQNLKNPLADMEVEKMEESAFNLETVSNIPCALLTGIRTLPVLEAKVPERPTSKCRDLPREEEKEKKKKKHKKRSRSRSQTPPSKYYSSSSSSKAKSRSHSKAKHSLPTAYRTVRHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCM6</accession>
    <name>A0A6I9YCM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Signal-induced proliferation-associated 1-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SIPA1L1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921809.1">
        <property type="nucleotide sequence ID" value="XM_014066334.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106548847"/>
    <dbReference type="KEGG" id="tsr:106548847"/>
    <dbReference type="CTD" id="26037"/>
    <dbReference type="OrthoDB" id="2499658at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06745">
        <property type="entry name" value="PDZ_SIPA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000002">
        <property type="entry name" value="Signal-induced proliferation-associated 1-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021818">
        <property type="entry name" value="SIPA1L_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF10">
        <property type="entry name" value="SIGNAL-INDUCED PROLIFERATION-ASSOCIATED 1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21022">
        <property type="entry name" value="Rap-GAP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11881">
        <property type="entry name" value="SPAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="6">
        <location>
            <begin position="596"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="950"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="136"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1071"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1144"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1247"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1307"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1361"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1425"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1615"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1710"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1071"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1151"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1188"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1261"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1277"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1379"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1617"/>
            <end position="1631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013921809.1"/>
        </source>
    </evidence>
    <sequence length="1773" mass="196556" checksum="6C99FEC8E848DB7C" modified="2020-10-07" version="1">MTSLKRSQTERAVATGISDVAPKVHSDDFYMRRFRSQNGSLGSSGMAPVGPPRNEGSHHLTSTPGVPKMGVRARIADWPPRKENVKENSRSSQEMDTSNCLDSMSSKSSPGSQGSSINLNSSDSVMLKSIQNTLKNKARQSENLDSRFLTPDGYHSSPRKALRRIRQRSNSDITLSELDVDSYDECISPSYKSGPSLHREYGSTSSIDKQGTSGGSFFDLLKDFKDDKSDQRGSGSSKFSELLIASGSKGSGFSLDVIDGPITQRENLRLFKEREKPLKRRSKSETGDSSIFRKLRNAKGEGDLGKTSDLEDSRSEDSVKPWTCPKCFAHYDVQSILFDLNEVAVNRHNVIKRRNTTTGASAAAVASLASGPLSHSASFNSPMGSTEELNSKGTLNVDQGDDKSNDLVLSCPYFRNEIGGEGERNISLTKSNSGSFSGCESASFESTLSSHCTNAGVAVLEAPKENLVLHLDRAKRYIVEHVDLGAYYYRKFFYQKEHWNYFGADENLGPVAVSIRREKPEEIKENSSQYSYRIIFRTSELTTLRGTVLEDAIPSTAKHCTARGLPLKEVLEHVVPELNIHCLRLAFNTPKVTEQLMKLDEQGLSYQLKVGIMYCKSGQSTEEEMYNNESAGPAFEEFLQLLGERVRLKGFEKYRAQLDTKTDSTGTHSLYTTYKEYEIMFHVSTMLPYTPNNKQQLLRKRHIGNDIVTIVFQEPGAESFSPKNIRSHFQHVFVIVRVHNPCTDNVCYSVAVTRSRDVPSFGPPIPKGITFPKSNVFRDFLLAKVINAENAAHKSEKFRAMATRTRQEYLKDLAEKNVTNTPIDPSGKFPFISLASKKKEKSKPHPGIELHSSGAVIWILHAKDYSKGFDIDCLLGISNEFVVVIEQDTKSVVFNCSCRDVIGWTSTDTSVKIFYERGECVSVESFKNPEDIKEIVKRLELVTKGCESVEMTLRRNGLGQLGFHVNYEGIVADVEPYGYAWQAGLRQGSRLVEICTVAVATLSHEQMIDLLRTSVTVKVVIIPPHEDCTPRRSFSEIHRMPVMEYKMNDGGVPYELKFPFRNNNKWQRNANKGQASHVQQMPSQMQSPITTRVASGKGEGKMPPPDRAANIPRSISSDGRPLESRRLSPGSDVYVTVSSMALARSQQCRNSPSNLSSSSETGSGGGTYRQKSMPEGFGMSRRSPASIDRQNTDTSGSGKSTPSWQRSEDSIVDQMEATCHLPAMSKVLPSFRESPTGRLMRQDPVVHLSPNKQGHSDSHYSSHSSSNTLSSNASSVHSDEKWYDSGDRTESELNSYNYLQGTSADSGIDTTSYGPSHSNSASLGNSATSPRSGLIKEKVASLWHSSSEVVSIADRTLEKDSHMMDRKTESSLSLDIHNKSQPSSNPLTRENSAFSLSDAGSHASTMGSRHSASSIVFTSARSSPKEELHSATSPQLAPSFSSSSSSSGPRTFYPRQGATSKYLIGWKKPEGTINSVGFMDSRKRHQSDGNEMAHPRLRASARDLRASPKRASKSNIEEELKKLIDLDSPTPESQKNFKSPFPTTPISRRALQRTLSDESIYSSQREHFFPSRASLLDQALPNDVLFTSTYPSLPKSLPLRRPSYTLGMKSLHGEFSASDSSLTDGQEQRTQPMPDPGLMPLPDSASDLDWSNLVDAAKAFEVQRASFFASSDENHRPISGASSNDQAEDQLSAQMKPYTDKESPPLASKVNQLESMLRILQEDLKKEKEDKANLQAEVQHLREDNLRLQEESHNASEKLKKFTEWVFNTIDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCS7</accession>
    <name>A0A6I9YCS7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BTB/POZ domain-containing protein KCTD14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KCTD14</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921971.1">
        <property type="nucleotide sequence ID" value="XM_014066496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCS7"/>
    <dbReference type="GeneID" id="106548992"/>
    <dbReference type="KEGG" id="tsr:106548992"/>
    <dbReference type="CTD" id="65987"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057890">
        <property type="entry name" value="KCTD7/14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF3">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25611">
        <property type="entry name" value="KCTD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="67"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921971.1"/>
        </source>
    </evidence>
    <sequence length="287" mass="32937" checksum="EFDCA5C94618CC74" modified="2020-10-07" version="1">MNIEELQGRDKNLERKTSLFLAITHQLLTGKPLRHQCQRRRGEKMSNSPEPKILGKQTPATLPTKCQIVELNIGGDIFTTTLSTLKKYPGTILAEMFGQAKPRTDSLGRVFIDRPGTYFKYILEYLRSGQVPLKDVEEVYKEATYYDIKPLIKQLEESPQIFGELVARKQFLARVPNYSENIELMIRIARAEAVASRKSSVIFCVMKTLEDVARCSDALNSLDTSKESVVQFGPWKAAPDNRDLLDCIKMDMDSKGYKTSFQGFIPDKGFRFSSKNDFFYKCLFQWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDA3</accession>
    <name>A0A6I9YDA3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox protein HMX2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HMX2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the HMX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922151.1">
        <property type="nucleotide sequence ID" value="XM_014066676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDA3"/>
    <dbReference type="GeneID" id="106549112"/>
    <dbReference type="KEGG" id="tsr:106549112"/>
    <dbReference type="CTD" id="3167"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000053">
        <property type="entry name" value="H6 family homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051300">
        <property type="entry name" value="HMX_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110:SF4">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="55"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="57"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013922151.1"/>
        </source>
    </evidence>
    <sequence length="181" mass="20301" checksum="C0374D5922AC9884" modified="2020-10-07" version="1">NSKGSGAALGSDRTPPFLSPSQPDFKEEKEKPFAPNSPACSADRQRDGMERQSGSAKKKTRTVFSRSQVYQLESTFDMKRYLSSSERACLASSLQLTETQVKTWFQNRRNKWKRQLSAELEAANMAHASAQTLVGMPLVFRDNSVLRVPVPRSIAFPAPLYYPGSNLSALPLYNLYNKLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDJ2</accession>
    <name>A0A6I9YDJ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Stabilin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106549181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922233.1">
        <property type="nucleotide sequence ID" value="XM_014066758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDJ2"/>
    <dbReference type="GeneID" id="106549181"/>
    <dbReference type="KEGG" id="tsr:106549181"/>
    <dbReference type="OrthoDB" id="8924685at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000005">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24038">
        <property type="entry name" value="STABILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24038:SF8">
        <property type="entry name" value="STABILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAS1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="5">
        <location>
            <begin position="116"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013922233.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="39110" checksum="9E5252318C9E5BAF" modified="2020-10-07" version="1">MVGKGPYTIFVPHADYLQKTTTFAEWEKAGCIKDLLRYHTVICQMLLSDDLESQDSITALSGHKIRVSVKENSLYLNDEVKIIESDITRENGVIHFIDGILAPYDLQHHSIPCNQSEKTIIEVAEAHGYNIYSKLLKETGLLNLVNNTLHQPFTMLWPTDEAFNSLPAEMQQWLYHKDHRSKLSAYLKGHMVRDIKVLASKLTELSSLRTLHGSTISFSCSKTNAGDIVVDNGNAKIVQRYMEFNVGIAYGTDHLLEPPDLGSRCDEFQTVEISHSLDQGSPTLDLWPSTIPRYASNWSTQTSEAPSMGCNQHAKPRPLQSMEKPFSTELVRGAKNVGSAALDKKKTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDJ5</accession>
    <name>A0A6I9YDJ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ryanodine receptor 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106546199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922241.1">
        <property type="nucleotide sequence ID" value="XM_014066766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDJ5"/>
    <dbReference type="GeneID" id="106546199"/>
    <dbReference type="KEGG" id="tsr:106546199"/>
    <dbReference type="OrthoDB" id="300855at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005790">
        <property type="term" value="C:smooth endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005219">
        <property type="term" value="F:ryanodine-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014808">
        <property type="term" value="P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006941">
        <property type="term" value="P:striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399">
        <property type="entry name" value="B30.2/SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399:SF9">
        <property type="entry name" value="RYANODINE RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013922241.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="13558" checksum="5997F08A361C2504" modified="2020-10-07" version="1">MVSCLHILAQTLDTRTVMKSGSEMVKAGLHAFFENAAEDLEKTSENLKLGKFTHSRTQIKGVSQNINYTTVALLPILTSIFEHVAQHNFGTDLLSFSANLLLLLDIEFHCLRFMDYVNSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDR0</accession>
    <name>A0A6I9YDR0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peroxisomal membrane protein 11A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PEX11A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922301.1">
        <property type="nucleotide sequence ID" value="XM_014066826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDR0"/>
    <dbReference type="GeneID" id="106549235"/>
    <dbReference type="KEGG" id="tsr:106549235"/>
    <dbReference type="CTD" id="8800"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF22">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013922301.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="28292" checksum="FA2FAB333E7D4CF5" modified="2020-10-07" version="1">MMEAFVHFTNQTQGRERLFRATQYSCMLLSYILERKSHREKVVMKLRLLESSMGSGRKMFRLGNIVHAMVATHQASQLPYAVPRFCLTASNVNRVMYFACDTVLWVKSIGLISELNQKKWRNWATKCYYYSLVINLIKDFYELCWRMKQTACSKKPEKALTLGKKKFQFLTNGELQTFLLLVFFTLKDYPPLLLDTVKNLCDIFSPLDRLGIYKTNPGVIGLCGLISSLVGILTVMRPHLRLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEG4</accession>
    <name>A0A6I9YEG4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transmembrane protein 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TMEM43</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM43 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922556.1">
        <property type="nucleotide sequence ID" value="XM_014067081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEG4"/>
    <dbReference type="GeneID" id="106549429"/>
    <dbReference type="KEGG" id="tsr:106549429"/>
    <dbReference type="CTD" id="79188"/>
    <dbReference type="OrthoDB" id="410725at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012430">
        <property type="entry name" value="TMEM43_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07787">
        <property type="entry name" value="TMEM43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013922556.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="32151" checksum="E3E21B2567E1F181" modified="2020-10-07" version="1">MYQWVEYEDSKEYEEDGEVKKETRYSYNTEWKSEVVNSRNFDREIGHKNPSAMAVESFTAVASDVQVGKFFLSRGLIEKINNFKQMSLSKLEDPHADVIRSGDYFFHSENPRRPEVGDLRVSFFYAGLSEDFSRMTLPDMVTIIARQQGDHLVPYQTKSGDVLNVLYPGELTAEEVFQKEHESNSMKTWGLRAAGWLSMFLGISLMTRIIYTLVDWFPVVRDLVNIGLKAFAFCLATSLSLLTISVGWLFYRPFWALLTALLAVVPILIARSQVQPKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFM8</accession>
    <name>A0A6I9YFM8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial fission factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106549571</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion outer membrane</location>
            <topology evidence="9">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the Tango11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922740.1">
        <property type="nucleotide sequence ID" value="XM_014067265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFM8"/>
    <dbReference type="GeneID" id="106549571"/>
    <dbReference type="KEGG" id="tsr:106549571"/>
    <dbReference type="OrthoDB" id="5986838at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090141">
        <property type="term" value="P:positive regulation of mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090314">
        <property type="term" value="P:positive regulation of protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039433">
        <property type="entry name" value="Mff-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008518">
        <property type="entry name" value="Mff/Tango-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16501:SF17">
        <property type="entry name" value="MITOCHONDRIAL FISSION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16501">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05644">
        <property type="entry name" value="Miff"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8 9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Mff-like" evidence="11">
        <location>
            <begin position="13"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013922740.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="22934" checksum="65249E4F49035806" modified="2020-10-07" version="1">MSTAAFQSPAAEMAEINRIQYEMEYTEGINQRMRVPEKLKVAPPNADLDQTCPEEGLPNASVTMQVPERIVVAGNSEDIPHSRPPDLDLLQSTPFKSLSLKTPPRVISLSDRPLDFLDLERPPPHTPQNEEIRTVGRLKRERSMSENATRQNGQLMRNEAVVTPSPQQVRVCSPQKFSEDGPSLYSTRGILSLIQSSTPRAYQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFP0</accession>
    <name>A0A6I9YFP0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="22">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">GAPDH</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 22">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-nitroso-L-cysteinyl-[GAPDH] + L-cysteinyl-[protein] = L-cysteinyl-[GAPDH] + S-nitroso-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:17089"/>
            <dbReference type="Rhea" id="RHEA-COMP:17090"/>
            <dbReference type="Rhea" id="RHEA-COMP:17091"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:149494"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66685"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 22">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="22"/>
    <dbReference type="RefSeq" id="XP_013923068.1">
        <property type="nucleotide sequence ID" value="XM_014067593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFP0"/>
    <dbReference type="GeneID" id="106549842"/>
    <dbReference type="KEGG" id="tsr:106549842"/>
    <dbReference type="CTD" id="2597"/>
    <dbReference type="OrthoDB" id="1152826at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000319">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000636">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF111">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12 22" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 19 22" id="KW-0520">NAD</keyword>
    <keyword evidence="19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="23">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="20">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047698"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048005"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
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    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
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    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013923068.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="35780" checksum="32593D49D255E55C" modified="2020-10-07" version="1">MVKVGINGFGRIGRLVTRAALQSGKVQIVAINDPFIDLSYMVYMFKYDSTHGRFSGSVQAENGKLVVNGHAITVFQERDPANIKWGEAGAEYVVESTGVFTTTEKASAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDKSLKVVSNASCTTNCLAPLAKVIHDNYGIVEGLMTTVHAVTATQKTVDGPSGKQWRDGRGAAQNIIPASTGAAKAVGKVIPDLNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKAASEGPMKGFLGYTEDQVVSSDFNGDTRSSIFDAAAGIALNDHFVKLISWYDNEFGYSNRVVDLMVYMASKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFS0</accession>
    <name>A0A6I9YFS0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC106549878</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2 chloride(in) + H(+)(out) = 2 chloride(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29567"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with NEDD4 and NEDD4L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Membrane</location>
            <topology evidence="24">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923108.1">
        <property type="nucleotide sequence ID" value="XM_014067633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFS0"/>
    <dbReference type="GeneID" id="106549878"/>
    <dbReference type="KEGG" id="tsr:106549878"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.20:FF:000001">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1280.20:FF:000001">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1280.20:FF:000002">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.20">
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002247">
        <property type="entry name" value="Cl_channel-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF7">
        <property type="entry name" value="H(+)_CL(-) EXCHANGE TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01116">
        <property type="entry name" value="CLCHANNEL5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0050">Antiport</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18 23" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20 24" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 24" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 24" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="25">
        <location>
            <begin position="622"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="25">
        <location>
            <begin position="712"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00029360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013923108.1"/>
        </source>
    </evidence>
    <sequence length="782" mass="86707" checksum="F75183EC2388709C" modified="2020-10-07" version="1">MLDFSMADDVPPLDRDMAEGFSSYNGGGMNGPRQVMDFLQEPLPGVGTYEDFNTIDWVREKSRDRDRHREIASKSKESTWALVHSVSDAFSGWLLMLLIGLWAGALAGLIDITAHWMTDLKEGVCLNGFWYNHEHCCWNSPQTTFQDRDKCPEWRTWAQLLTGREEGGALGYVLNYFLYVLWALLFSFLAVSLVRGFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLAVSSGLSLGKEGPLVHVACCCGNILCHLFTKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEFHSPWHLLELAPFILLGIFGGLWGAFFIRSNIAWCRRRKTTKLGRYPVLEVLVVTALTALLAFPNKYTRMSTSELISELFNDCSLLDSSQLCDYLSAPNSTQGDDLPDRAAGPGLHVATWQLMLALLLKIFITIFTFGMKVPSGLFIPSMAVGAIAGRLLGVGMEQLAYHHHDWLIFKGWCSPGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADAIGREGIYDAHIRLNGYPFLEAKEEFTHKTLAMDVMRPRRSDPALTVLTQDTMTVEDVEAIINETTYSGYPVVVSRESQRLVGFVLRRDLVISIENARKKQEGIVSSSVIYFTDHCPPLPPCSPSMLKLRGILDLSPFTVTDQTPMEIVVDIFRKLGLRQCLVTHNGKLLGIITKKDVLKHIAQMANQDPDSILFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGD5</accession>
    <name>A0A6I9YGD5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTPase IMAP family member 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106550006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923256.1">
        <property type="nucleotide sequence ID" value="XM_014067781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGD5"/>
    <dbReference type="GeneID" id="106550006"/>
    <dbReference type="KEGG" id="tsr:106550006"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF170">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="14"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013923256.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="25791" checksum="1C51079B34DC1E43" modified="2020-10-07" version="1">MGAAESAYQEAGSVADLRIVMVGKTGNGKSATGNTILGREAFTSKMEATSVTDECKSETKVLANGRTLTVIDTPGFFDTKYPEPVTLAEIKKCVSLCSPGPHVIIQVIRMGHFSKEEMKVSEIINSIFSMKAKAYLVILFTRKEDLEGRPLQEVLSEGGKSLEPLRKQIKSCGNRYLAFNNKAEGKERQDQVDELIRMVDGLRGKNWLQPYYTKEMMEEDKKKAPGWLCTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGN1</accession>
    <name>A0A6I9YGN1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">N-acetylglucosamine-1-phosphotransferase subunits alpha/beta</fullName>
            <ecNumber evidence="17">2.7.8.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">GlcNAc-1-phosphotransferase subunits alpha/beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Stealth protein GNPTAB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">GNPTAB</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(4)-[alpha-D-mannosyl-(1-&gt;2)-alpha-D-mannosyl-(glycan)]-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-[6-(N-acetyl-alpha-D-glucosaminyl-1-phospho)-alpha-D-mannosyl-(1-&gt;2)-alpha-D-mannosyl-(glycan)]-L-asparaginyl-[protein] + UMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13581"/>
            <dbReference type="Rhea" id="RHEA-COMP:14507"/>
            <dbReference type="Rhea" id="RHEA-COMP:14508"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:140357"/>
            <dbReference type="ChEBI" id="CHEBI:140369"/>
            <dbReference type="EC" id="2.7.8.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the stealth family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.17" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013923055.1">
        <property type="nucleotide sequence ID" value="XM_014067580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGN1"/>
    <dbReference type="GeneID" id="106549829"/>
    <dbReference type="KEGG" id="tsr:106549829"/>
    <dbReference type="CTD" id="79158"/>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003976">
        <property type="term" value="F:UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046835">
        <property type="term" value="P:carbohydrate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016256">
        <property type="term" value="P:N-glycan processing to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21599">
        <property type="entry name" value="RRM1_GNPTAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21600">
        <property type="entry name" value="RRM2_GNPTAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.320:FF:000002">
        <property type="entry name" value="N-acetylglucosamine-1-phosphotransferase subunits alpha/beta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041536">
        <property type="entry name" value="GNPTAB_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035993">
        <property type="entry name" value="Notch-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047141">
        <property type="entry name" value="Stealth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031358">
        <property type="entry name" value="Stealth_CR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021520">
        <property type="entry name" value="Stealth_CR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031357">
        <property type="entry name" value="Stealth_CR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031356">
        <property type="entry name" value="Stealth_CR4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24045">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24045:SF0">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-1-PHOSPHOTRANSFERASE SUBUNITS ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18440">
        <property type="entry name" value="GlcNAc-1_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00066">
        <property type="entry name" value="Notch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17101">
        <property type="entry name" value="Stealth_CR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11380">
        <property type="entry name" value="Stealth_CR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17102">
        <property type="entry name" value="Stealth_CR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17103">
        <property type="entry name" value="Stealth_CR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01137">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00004">
        <property type="entry name" value="NL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90193">
        <property type="entry name" value="Notch domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51912">
        <property type="entry name" value="DMAP1_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50258">
        <property type="entry name" value="LNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1213"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="domain" description="LNR" evidence="25">
        <location>
            <begin position="438"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="DMAP1-binding" evidence="26">
        <location>
            <begin position="699"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="24">
        <location>
            <begin position="1008"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013923055.1"/>
        </source>
    </evidence>
    <sequence length="1259" mass="144275" checksum="73DEE29B636346AE" modified="2020-10-07" version="1">MLLKLLQRQTYTCLSHRYGPCLCLGGIVLLIVSALQFGEVVLEWSRDQYHVLFDSYRDNVAGKSFQNRLCLPMPIDVVYTWVNGTDVELIKELQQVRKQMEEEQKTIREILGKNVTEPTKKSEKQLECLLTHCIKVPMLVLDPALPNNITLKDLPSVYPLLRSANNIFSVAKPKNPSTNVTVVVFETAKEAEDAHSLMLKESIKQTAWRGYLTTDKEVPGLVIMQDLVLLKGFPLTYKETNQLRAKLPEVLVSKIKQLQLYSEASVALLQLIDPKVFQELNKQAKKNMTIEGKELTLNPAYLLWDLSAISQSKQDEDISPSRFEDNEELRYSLRSIERHAPWVRHIFIVTNGQIPSWLNLDSPRVTIVTHQDIFQNMSHLPTFSSPAIESHIHRIKGLSQKFVYLNDDVMFGKDVWPDDFYSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKACNNSACDWDGGDCIGNSVGSRYGAGAGAVGGIGNVPAWQFGAGISGVSYCNQGCANSWLADKFCDQACNVPSCGFDAGDCGPDHFGELYRVTLQKNQTHYIIPKGESLPYFSFAEIAKKGLEGAYSDNPIIRHASIANKWKTIHLLMHSGMNASVIHFNLTFQSKDEQEFKMQITLEVDTREESKLNTTATQQPEGDLKATTSVPEAEMIFEDIPEERRFPRVKKRLNGTFNNLDEETVIQAVNMSALPEDVKIALQNLELQLIVGDITQKGYNLSKAALLRSLQLMPTERKMGLNDLEESRKRDKGLKIVGKTMLWPRPSQSDNEGKIVATDKMHNISLPGFAPTSSILVEMKVTNDFSLSYRSTVKPKPTSVVRNVDKKHGSQDKALNILMQKEIQERKIEQRKKKEIDVNPPFSEADIAEGPRRKLQYYTGNYQGFLPWEKHKYFQDLLDEEESLKKELAYFIESKVVGRQLKDTFADSLRYVNKLLNGKFGFTSRKVPAHMPHMIDRKVMQELQDLFPEEFDKTSFHKVRHSEDMQFAFSYFYYLMSAVQQLNISQVFDEIDTDHSGILSDREIRTLATRIHELPLSLQDLTGLEQMLINCSKSLPLNITQINIIPPTQEAYYDPNLPPVTKGLLMNCKLVTDRIRKTYKDKNKYRFEIMGEEEVAFKMIRTNVSHVVGQLDDIRKNPRKFVCLNDNIDHNHKDAQTVKAVLRDFYESMFPIPSQFELPREYRNRFLHTQELQEWRAYRDKLKFWTHCVLVTLIVFTVTSFFAEQLIALKRKIFPRRRIQKEVCYERMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGX3</accession>
    <name>A0A6I9YGX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein-methionine sulfoxide oxidase MICAL1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106550209</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923548.1">
        <property type="nucleotide sequence ID" value="XM_014068073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGX3"/>
    <dbReference type="GeneID" id="106550209"/>
    <dbReference type="KEGG" id="tsr:106550209"/>
    <dbReference type="OrthoDB" id="20799at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF35">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51848">
        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BMERB" evidence="4">
        <location>
            <begin position="67"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013923548.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="25541" checksum="CA56DCACC94C92EC" modified="2020-10-07" version="1">MQSKYSIFCNHSCFSLADFLEVDSDSEEEEEEEEEGNLDPGPSMHFLSLTEETQYPTWKKTLLRRSKEAEMKRFCKAQAIQRRLEEIEVTFRELEKQGIKVEQSLREEAGTESELKKQWMNQLLTLIQKKNSLMSEESDLMIDVQELKLEEQQWQLDQELRRYYNLEDSLKTSEDYESEKIILSQLVAIVNQRSALIEMQERKRLSELSELSMHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHY2</accession>
    <name>A0A6I9YHY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106550560</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923968.1">
        <property type="nucleotide sequence ID" value="XM_014068493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHY2"/>
    <dbReference type="GeneID" id="106550560"/>
    <dbReference type="KEGG" id="tsr:106550560"/>
    <dbReference type="OrthoDB" id="9975554at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="43"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013923968.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="35508" checksum="A26F5937F6C0E336" modified="2020-10-07" version="1">MEKNQENQTNFVGFILLGFSSSPEFQGLLFPVFLCMYLLCLLGNLVIILLIFSSASLLHAPMYFFLCHLSLADLGLSSTIAPQMLQNLLSCTNTISFSGCLTQVYFFVSFCAADNFLLASMAFDRYVAICRPLHYATIMSYRFCIQLAAVCWLLAYAHSLLYVLSMCNFKFCASRKIPHFFCDLHPLLKLSCSDTSSVEMLLVSEGTAILLGPLLLIIFSYLFIAFKVLKIPPGHRKYKAFSTCLSHLTTVALFYGTLLGIYIRPSSTYAGSTLKIASILYTVVTPMLIPFIYSLRNRTMHEAMKRILRGWFQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJE7</accession>
    <name>A0A6I9YJE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spermatogenesis-associated protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SPATA20</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923900.1">
        <property type="nucleotide sequence ID" value="XM_014068425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJE7"/>
    <dbReference type="GeneID" id="106550498"/>
    <dbReference type="KEGG" id="tsr:106550498"/>
    <dbReference type="CTD" id="64847"/>
    <dbReference type="OrthoDB" id="1923667at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="2"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923900.1"/>
        </source>
    </evidence>
    <sequence length="710" mass="80645" checksum="2ABC0B6DD10AB984" modified="2020-10-07" version="1">MDEPVDWYPWGQEAFDKAKREDKLIFLSVGYSTCHWCHVMEQESFQNEEIGQILNENFVCIKVDREERPDVDKVYMTFVQATSSGGGWPMSVWLTPDLKPFVGGTYFPPEDGIYQVGFRTVLLRILEQWKRNKSTLLENSQKILSALLARTDVGVRGDEAPPSLKEVTTRCFQQLSETYDEEYGGFSESPKFPTPVNLNFLFSYWALQRSNTEGARALQMALHTLKMMAYGGIHDHIAQGFHRYSTDQRWQVPHFEKMLYDQGQLAVVYARAFQISGDEFFADTAADILLYVSRDLSDTSGGFYSAEDADSYATVQSEKKQEGAFCVWTAEEIRQLLPDPVEGIQEEKIVADVFMHHYGMKEDGNVNPMKDPHNELKRKNVLIMQYSLELTAARFGLGLEELKTLLVKSRQKLYKARTQRPRPHLDSKMLASWNGLMISGFAQCGTILGKKEYIDRAVCAASFLQNHMFNASSGKLLRTCYQGQGNTVTHSSVPIHGYLEDYVFVIQALFDLYEATLNPSWLEWAVQLQQKQDELFWDPKGFAYFSTEAADPSLLFRIKDDQDGAEPSPNSVAVSNLLRAASYTDCEEWVKKAGQILTVFSERLVKIPVVLPEMARATAAFYLTLKQVVICGDLKGEDTQELLHCYYSTYTPNRVLILADGDTTGFLYHQLGFLSSLEKKDGKATAYVCENFTCSLPVTSSQELRRRLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKD1</accession>
    <name>A0A6I9YKD1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Small nuclear ribonucleoprotein Sm D3</fullName>
            <shortName evidence="13">Sm-D3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">snRNP core protein D3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SNRPD3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924828.1">
        <property type="nucleotide sequence ID" value="XM_014069353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKD1"/>
    <dbReference type="SMR" id="A0A6I9YKD1"/>
    <dbReference type="GeneID" id="106551285"/>
    <dbReference type="KEGG" id="tsr:106551285"/>
    <dbReference type="CTD" id="6634"/>
    <dbReference type="OrthoDB" id="6425924at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013924828.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="13916" checksum="59A6E78E860AA3E0" modified="2020-10-07" version="1">MSIGVPIKVLHEAEGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQVYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGMGRGNIFQKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKM7</accession>
    <name>A0A6I9YKM7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Testis-expressed sequence 2 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106551356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924928.1">
        <property type="nucleotide sequence ID" value="XM_014069453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKM7"/>
    <dbReference type="GeneID" id="106551356"/>
    <dbReference type="KEGG" id="tsr:106551356"/>
    <dbReference type="OrthoDB" id="26740at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21675">
        <property type="entry name" value="SMP_TEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924928.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="25025" checksum="C902B4D5AA27C0D1" modified="2020-10-07" version="1">MTLETKMNLTKLGKEPLGEALKSGEIGKEGFRPRAYYLADSDEESSSAGSSEEDDVPEASGSEKALVAGAEGYGAVSRTSKIMRFVDKITKSKYFQKATETEFIKKKIEEVSNTPLLLTVEVQECKGMLAINIPPPPTDRIWYGFRKPPYLELKARPKLGEREVTLVHVTEWIEKKLEQEFQKVFVMPNMDDLYIPLMHSVTDPRSSMCPLKDSEDTELEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKU2</accession>
    <name>A0A6I9YKU2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dedicator of cytokinesis protein 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106551297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924852.1">
        <property type="nucleotide sequence ID" value="XM_014069377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKU2"/>
    <dbReference type="GeneID" id="106551297"/>
    <dbReference type="KEGG" id="tsr:106551297"/>
    <dbReference type="OrthoDB" id="9189685at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dedicator of cytokinesis C/D N-terminal" evidence="1">
        <location>
            <begin position="49"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924852.1"/>
        </source>
    </evidence>
    <sequence length="104" mass="11629" checksum="19A13C8239595AB5" modified="2020-10-07" version="1">MAAPERRAFAHRINRTVAAEVRKHVCREYGNSPQLSKKRGGTLQSLPLTEVVEPVDFEKYLNTHLPDAEPGPLSDLIEFPADDLEVSHEPRECRTLEPGVPEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YL50</accession>
    <name>A0A6I9YL50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Oxidative stress-responsive serine-rich protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Oxidative stress-responsive protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Peroxide-inducible transcript 1 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106551512</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925108.1">
        <property type="nucleotide sequence ID" value="XM_014069633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YL50"/>
    <dbReference type="GeneID" id="106551512"/>
    <dbReference type="KEGG" id="tsr:106551512"/>
    <dbReference type="OrthoDB" id="10045817at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008494">
        <property type="entry name" value="DUF776"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31383">
        <property type="entry name" value="OXIDATIVE STRESS-RESPONSE SERINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31383:SF2">
        <property type="entry name" value="OXIDATIVE STRESS-RESPONSIVE SERINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05604">
        <property type="entry name" value="DUF776"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013925108.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="31569" checksum="127555575057A0E4" modified="2020-10-07" version="1">MLENEENLGAAFKKLRVDSERSTASLPVSEGASPQTFVGSTDESKVKMTFGSKESWHWCLRKPLRGTIRTQRCRRSKSPVLHPPKFIHCTKQMASGSQSMHKNLTDAFQSNKDLDVPKKFSENELGSHPLEFGKKESVVKDSEASMIGIPKNISEYIGNVLPTEILKVSDLSDFQSVSEQNKGNFCACVKNECQCKQWQNLNVYMFSDIQNSLRCVSERAAHVQNNPQLTSRISSSSLKSCSEQARAHVDDVTIEDLSGYMEYFLHIPKEMSHMAEMMYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMD9</accession>
    <name>A0A6I9YMD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insulin-like growth factor-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IGFBP1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Binds equally well IGF1 and IGF2. Interacts with integrin ITGA5:ITGB1. Interacts with VHL; this interaction inhibits HIF1A degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925296.1">
        <property type="nucleotide sequence ID" value="XM_014069821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMD9"/>
    <dbReference type="GeneID" id="106551679"/>
    <dbReference type="KEGG" id="tsr:106551679"/>
    <dbReference type="CTD" id="3484"/>
    <dbReference type="OrthoDB" id="9926277at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031994">
        <property type="term" value="F:insulin-like growth factor I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031995">
        <property type="term" value="F:insulin-like growth factor II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043567">
        <property type="term" value="P:regulation of insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.20:FF:000001">
        <property type="entry name" value="Insulin-like growth factor binding protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000002">
        <property type="entry name" value="Insulin-like growth factor-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022322">
        <property type="entry name" value="IGFBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022321">
        <property type="entry name" value="IGFBP_1-6_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017891">
        <property type="entry name" value="Insulin_GF-bd_Cys-rich_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551:SF6">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01976">
        <property type="entry name" value="IGFBPFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01977">
        <property type="entry name" value="IGFBPFAMILY1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00222">
        <property type="entry name" value="IGFBP_N_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026848390" description="Insulin-like growth factor-binding protein 1" evidence="10">
        <location>
            <begin position="23"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="12">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="11">
        <location>
            <begin position="174"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925296.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="28605" checksum="0782B309227DB960" modified="2020-10-07" version="1">MTYPGFSCQLLLLFLGPGFSLGAALHLMHCAPCTEEKLRLCPPVSANCPETSHQPGCSCCHSCALQLGEPCGIYTARCSQGLSCQVQVDQARPLYVLTQGQGTCLPIAGRTETAESVEPEDMPTEGDEITADHLVNYEVAFSAEQDKSASWDANNIYESLRADKPAEVKKRREQGPCQKELYKALDKLAKAQQTTGGQLYRFYLPNCNKNGFYHSKQCETSLDGNPASCWCVYPKNGKRIPESPEMMGNLECEQFISSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMR7</accession>
    <name>A0A6I9YMR7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Teneurin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">Protein Odd Oz/ten-m homolog 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Tenascin-M2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Teneurin transmembrane protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="41" type="primary">TENM2</name>
    </gene>
    <organism evidence="40 41">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Induces gene transcription inhibition.</text>
    </comment>
    <comment type="function">
        <text evidence="34">Involved in neural development, regulating the establishment of proper connectivity within the nervous system. Acts as a ligand of the ADGRL1 and ADGRL3 receptors that are expressed at the surface of adjacent cells. Promotes the formation of filopodia and enlarged growth cone in neuronal cells. Mediates axon guidance and homophilic and heterophilic cell-cell adhesion. May function as a cellular signal transducer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">PML body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Postsynaptic cell membrane</location>
            <topology evidence="27">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Presynaptic cell membrane</location>
            <topology evidence="28">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="35">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925693.1">
        <property type="nucleotide sequence ID" value="XM_014070218.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106552030"/>
    <dbReference type="KEGG" id="tsr:106552030"/>
    <dbReference type="CTD" id="57451"/>
    <dbReference type="OrthoDB" id="442731at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000176">
        <property type="entry name" value="Si:ch211-12m10.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000016">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000021">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000026">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000474">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000003">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.180.10.10:FF:000001">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000061">
        <property type="entry name" value="teneurin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022385">
        <property type="entry name" value="Rhs_assc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009471">
        <property type="entry name" value="Ten_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056820">
        <property type="entry name" value="TEN_TTR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057629">
        <property type="entry name" value="Teneurin1-4_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03696">
        <property type="entry name" value="Rhs_assc_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF8">
        <property type="entry name" value="TENEURIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23093">
        <property type="entry name" value="GBD_Tenm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06484">
        <property type="entry name" value="Ten_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23538">
        <property type="entry name" value="Teneurin_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25020">
        <property type="entry name" value="TTR_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51361">
        <property type="entry name" value="TENEURIN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="26" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="21 35" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 35" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="23" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20 37" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="40" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0678">Repressor</keyword>
    <keyword evidence="18" id="KW-0770">Synapse</keyword>
    <keyword evidence="22" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12 37" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 37" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Teneurin N-terminal" evidence="39">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="38">
        <location>
            <begin position="544"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="38">
        <location>
            <begin position="578"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="38">
        <location>
            <begin position="673"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="36">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="36">
        <location>
            <begin position="105"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="36">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="36">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="36">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="36">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="35">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="35">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="35">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="35">
        <location>
            <begin position="698"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00037805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00046300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00057432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00068165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00077041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00081436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00083964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00093288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS51361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="RefSeq" id="XP_013925693.1"/>
        </source>
    </evidence>
    <sequence length="2641" mass="293490" checksum="BF63A09466E30DF7" modified="2020-10-07" version="1">MLHTANKGRKASTETDFHTQLSETLKDRQTGWQQLAETKNSLIRRPIPPTSSSSLLPSAQLPSSHNPPQVSCQMPLLDSNTSHQIMDTNPDEEFCPNSYLLRACTGPQQTSSSGPSNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRNQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGTTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRNAFKLKKPSKYCSWKCAALSAIAAAILLAILLAYFIAMHMLGLNWQLQPADGHTFNNGIRTGLPGSDDVATMPSGGKGPWATRNNSIDSGEAEVGRRITQEVPQGVFWRSQIHIAQPQFLKFNISLGKDALFAVYIRRGLPPSHVQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEVVSFSTVILDSVQDCPRNCHGNGECVSGICHCFPGYHGADCAKAACPVLCSGNGQYTKGACLCYSGWKGPECDIPISQCIDPSCGGHGSCIEGNCVCSVGYKGENCEEVDCLDPSCSNHGVCVNGECLCSPGWGGLNCELPRAQCPDQCSGHGTYLTDTGLCNCDPNWMGPDCSVEVCAVDCGTHGVCIGGACRCEEGWTGIACDQRVCHPRCTEHGTCKDGKCECREGWNGEHCTIDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLIDCLDPDCCLQSTCQNSLLCRGSRDPLDIIQQSHSGSEAVKSFYDRIKLLVGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLTLKTEENSIPSCDLSGFIRPDPVIISSPLSTFFSDAPGRNSIVPETQVLHEEIEIPGANIKLCYLSSRTAGYRSLLKITMTQSLVPLNLIKVHLMVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQKVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHVLNIKSGILHKGSGENQFLTQQPAVITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLFVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIFRVKSLTGAKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAVDATLMSPRGIAVDKYGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVTQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHAGILYISETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYAGDDAYATDAILNTPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNSFNQYEIASPREQELYIFNIDGIHQYTLSLVTGEYLYNFTYSADNDVTELVDNNGNSLKIRRDTNGAARHFLMPDNQIVTLAVSTNGGLKAVSTQTLELGLMTYHGSSGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCSNGTLRVMYANGMSISFHSEPHVLAGTVTPAIGRCNISLPMENGLNSIEWRLRKEQIKGKITVFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQLGRPFLWLPSSGLAAVNVSYFSNGRLAGLQRGAMSERTEIDKQGRIIARMFADGKVWSYTYLEKSMVLLLQSQRQYIFEYDSSDRLHAVTMPSIARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIAYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPIISEIPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVTKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPNWRYSYDLNGNLHLLNPGNSIRLMPLRYDLRDRITRLGDMQYKIDDDGFLCQRGSDTFEYNSKGLLTRAYNKANGWSVQYRYDGLGRRASYKTNLGHHLQYFYADLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYIASDNTGTPLAVFSINGLMIKQLQYTAYGEIYHDSNPDFQIVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSNELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPSYELSESQACEHGQLITGVQQTTERHNQAFMALEGQVISKKLHASRREKAGHWFATTTPIIGKGIMFAVKEGKVTTGISSTATEDSRKIASVLNNAYYLDKMHYSIEGKDTHYFVKIGSSDGDLVTLAMTSGRKVLDSGVNVTVSQPTLLINGRTRRFTNVEFQYSTLLINIRYGLTPDTLDEEKARVLDQARQRALGSAWAKEQQKARDGKEGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMU0</accession>
    <name>A0A6I9YMU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphodiesterase</fullName>
            <ecNumber evidence="12">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106551821</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:16958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14653"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57867"/>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <dbReference type="EC" id="3.1.4.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:14654"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="12">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013925451.1">
        <property type="nucleotide sequence ID" value="XM_014069976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMU0"/>
    <dbReference type="GeneID" id="106551821"/>
    <dbReference type="KEGG" id="tsr:106551821"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013706">
        <property type="entry name" value="PDE1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08499">
        <property type="entry name" value="PDEase_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="2" id="KW-0140">cGMP</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="13">
        <location>
            <begin position="126"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="203"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925451.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="48938" checksum="36337038776850CD" modified="2020-10-07" version="1">MDDCVTIKKKHLQRPIFRLRCLVKQLEKGDVNVVELKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQSDSVPLEVRDWLASTFTRKMGMTKRRPEEKPKFRSIVHAVQAGIFVERMYRRTSSMIGLAYPAEVIATFKDVDKWSFNIFSLNEASGGHSLKFMMHELFTRYDLLSRFKIPVPCLISFGEALEIGYSKYKNPYHNLIHAADVTHTVHCIMLLTGIMHWLTELEILAMIFAAAVHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYKIMQDEEMNILVNLTKDEWRDLRNLVVEMVLSTDMSGHFQQIKTMRHTLQQTEGIDKARAMSLMLHAADISHPSKLWVLHYRWTKALMEEFFRQGDKEAELGLPFSPLCDRKSTMVAQSQIGKGWFLLKKSYNERKKCSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNC7</accession>
    <name>A0A6I9YNC7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein KIAA0408 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">KIAA0408</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925661.1">
        <property type="nucleotide sequence ID" value="XM_014070186.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925662.1">
        <property type="nucleotide sequence ID" value="XM_014070187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNC7"/>
    <dbReference type="GeneID" id="106552009"/>
    <dbReference type="KEGG" id="tsr:106552009"/>
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    <dbReference type="OrthoDB" id="10036174at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027882">
        <property type="entry name" value="SOGA1/2-like_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15705">
        <property type="entry name" value="MCG7194, ISOFORM CRA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15705:SF1">
        <property type="entry name" value="RIKEN CDNA 9330159F19 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14818">
        <property type="entry name" value="SOGA1-2-like_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOGA 1/2-like coiled-coil" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="472"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="617"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013925662.1"/>
        </source>
    </evidence>
    <sequence length="639" mass="72765" checksum="2DB137970C234602" modified="2020-10-07" version="1">MDLQTQLERMTEKNWSKEKMELLERFDSERKEWECQWKVMQKKIEELYQEVKLRRENNMSISENKPIHNKAEQQTCSPTLEWSDPPGLYSCSPDKESLQSQAEKENKCVKNKKRNHAFAKDHLTFQSSKESEDCVDLKTTKNYTQDLNIALKELAKVSEDLCTYQEEIRKKANHRRKKMFPFFGESIESGNALITQETSPAYYKSPPLAAVASETEKQNNRKNLISTNRHLKETPSTALSGVEETSFLPWQRREGPPVPPRSTSRHLTSSLADVSEALAKHVEQRNSCKTQENPDGSPFIYPAAPLTMSDECKSLSGPTENQDQYNDHKLPAAILHSVWSCEASKIVNGPENGSSRHALQKGSSDGYMMTPKQHPKFQSSVHCTSNPYIAACDTLEDSGYETGKTQRNETLEAKIDEFNRTVFHTDKGKKCLLQNQTELPGVPKAHCGLQNSLNNRTPNEESTYVLNSKLSSKKEEKANRPSKDVKTKRQQKQINGVLSGYQHMLHEHDWRPSNLSGRPRSADSRSNYGVVEKLLKNYEKQVQSEAEFTEGSFRKIGHYPVIQIEQGKQEQQKNSVRHLGLYGKEKQKFPEVSLAAKQSNGKAFSRPARPANRRLPSRWASRSPSAPPAMRRTLLNEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNH9</accession>
    <name>A0A6I9YNH9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Disheveled-associated activator of morphogenesis 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106551838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925474.1">
        <property type="nucleotide sequence ID" value="XM_014069999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNH9"/>
    <dbReference type="GeneID" id="106551838"/>
    <dbReference type="KEGG" id="tsr:106551838"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001914">
        <property type="entry name" value="Disheveled associated activator of morphogenesis, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF16">
        <property type="entry name" value="DISHEVELED-ASSOCIATED ACTIVATOR OF MORPHOGENESIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="1">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013925474.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="23657" checksum="E91D2FD904F8DCF9" modified="2020-10-07" version="1">MESYEVHEDVEQNGYHHWTQNPSVWFVTRFIDLDGLSCILNFLKSMDYETSESRIHTSLIGCIKALMNNSLGRAHVLAHSESINVIAQSLSTENIKTKVAVLEIMGAVCLVPGGHKKVLEAMVHYQKYASERTRFQTLINDLDRSTGRYQDEVSLKTAIMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNP3</accession>
    <name>A0A6I9YNP3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Methionine-R-sulfoxide reductase B1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MSRB1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Acts as a regulator of actin assembly by reducing methionine (R)-sulfoxide mediated by MICALs (MICAL1, MICAL2 or MICAL3) on actin, thereby promoting filament repolymerization. Plays a role in innate immunity by reducing oxidized actin, leading to actin repolymerization in macrophages.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 20">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (R)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:21260"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58773"/>
            <dbReference type="EC" id="1.8.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926103.1">
        <property type="nucleotide sequence ID" value="XM_014070628.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106552374"/>
    <dbReference type="KEGG" id="tsr:106552374"/>
    <dbReference type="CTD" id="51734"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052150">
        <property type="entry name" value="MsrB_Met_sulfoxide_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46755">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46755:SF5">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0711">Selenium</keyword>
    <keyword evidence="10 19" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="17">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="19">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013926103.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="13036" checksum="CA7C55A1281BC786" modified="2020-10-07" version="1">MCAYLIPPCHIYSSFSPSPSLLTGIYVCSKCGYELFSSQSKFLHSSPWPAFTHPIHSDSISKYLERPGAFKVSCGKCGNGLGHEFLDDGPQKGQSRFUIFSSSLTFVHKAQSGKNMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPC9</accession>
    <name>A0A6I9YPC9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 17">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11 17">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">USP5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 23">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 17">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11 17"/>
    <dbReference type="RefSeq" id="XP_013926016.1">
        <property type="nucleotide sequence ID" value="XM_014070541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPC9"/>
    <dbReference type="GeneID" id="106552273"/>
    <dbReference type="KEGG" id="tsr:106552273"/>
    <dbReference type="CTD" id="8078"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14383">
        <property type="entry name" value="UBA1_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14386">
        <property type="entry name" value="UBA2_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000016">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000070">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000256">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000033">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000042">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041812">
        <property type="entry name" value="UBP5_UBA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF103">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 11 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11 17" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 17" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11 17" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 11 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="20">
        <location>
            <begin position="173"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="19">
        <location>
            <begin position="324"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="650"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="718"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="814"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="219"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="193"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013926016.1"/>
        </source>
    </evidence>
    <sequence length="854" mass="95656" checksum="32376C1A48B6FD59" modified="2020-10-07" version="1">MAELSEALLSVLPSIRVPKAGDRVHKDECAFSFDTPESDGGIYICMNTFLGFGKQYVTKHYEKTGQRVYLHLKKTRRLKEENNSSAGDPPRKKPTRLAIGVEGGFDLPEEKYEYDEHVKIVILPEHLDIPQDGLDGLPDMVKDRISNAIEAILTADSASRKQEVQAWDGEVRRVSRHAFSLQQLQNGIRIPPCGWKCSKCDMRENLWLNMTDGSISCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPNLAEHLAHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVQLKPLYGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQSAPTDPTQDFSTQVAKLGHGLLSGEYSKPALSVGEQQLDQKGIQEGIAPRMFKSLVGKGHPEFSTNRQQDAQEFFLHFINMVERNCRSSENPNEVFRFLVEEKLKCLATEKVKYTQRVDYIMQLPVPMEAALNKDELLEYEEKKRLAEEEKQPLPELVRAKVSFTSCLEAYGAPEQVDDFWSTALQAKSVALKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPDELDISALHGTGLQPEEEEMPDIAPPLVTPDEPKGSLGFYGNEDDDSFCSPHFSSPTSPMLDESVITQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLVLPGSSGPGSTITCPDPPSEDSVATIVSMGFSRDQAIKALKATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAAESISESIPVGPKVRDGPGKYQLFAFVSHMGTSTMCGHYVCHIKKDNRWVIYNDQKVCASEKPPKDLGYIYFYQRIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQF4</accession>
    <name>A0A6I9YQF4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">F-box only protein 48</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FBXO48</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926149.1">
        <property type="nucleotide sequence ID" value="XM_014070674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQF4"/>
    <dbReference type="GeneID" id="106552412"/>
    <dbReference type="KEGG" id="tsr:106552412"/>
    <dbReference type="CTD" id="554251"/>
    <dbReference type="OrthoDB" id="10257471at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042627">
        <property type="entry name" value="FBXW2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436">
        <property type="entry name" value="F-BOX/WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="39"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013926149.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19817" checksum="6E93B4AA48D13AAB" modified="2020-10-07" version="1">MKNGSKQKSSKQNQQTVETTTCKKLFFKSSKLKKHNSQEDFVKLFPLEVSLKIFSKLDIRSLCNAAITCKSWNQTIEHCDDLWKHHCLNIRAVCQREVDSDRGKGYSWKVTLHRNYWKSKVKYEWLSGKYSNIQSRSNLPEKCMYPMDVDTWGEILEAELERVLYRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR37</accession>
    <name>A0A6I9YR37_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">YIF1A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926100.1">
        <property type="nucleotide sequence ID" value="XM_014070625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR37"/>
    <dbReference type="GeneID" id="106552372"/>
    <dbReference type="KEGG" id="tsr:106552372"/>
    <dbReference type="CTD" id="10897"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF2">
        <property type="entry name" value="PROTEIN YIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013926100.1"/>
        </source>
    </evidence>
    <sequence length="161" mass="17910" checksum="1FE5427807AD578F" modified="2020-10-07" version="1">MAYPPGYGGPVSKHRTRIPPVPQSPDAPQLFDDTSSGYGPPQAGYPPPGIDMSFNQIFADPVANVAMAYGSSIASQGKEIVHKEIHRFVPINKLKYFFAVDTTYVMKKLGLLIFPYVHQNWEVRYHRDIPLTPRQDINAPDLYIPSMAFITYILLAGMALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRB3</accession>
    <name>A0A6I9YRB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U8 snoRNA-decapping enzyme</fullName>
            <ecNumber evidence="8">3.6.1.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">IDP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Inosine diphosphate phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Nucleoside diphosphate-linked moiety X motif 16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">m7GpppN-mRNA hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106552430</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>IDP + H2O = IMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35207"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="EC" id="3.6.1.64"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:35208"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>dIDP + H2O = dIMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35211"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:62286"/>
            <dbReference type="EC" id="3.6.1.64"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:35212"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Nudix hydrolase family. NUDT16 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.64" evidence="8"/>
    <dbReference type="RefSeq" id="XP_013926175.1">
        <property type="nucleotide sequence ID" value="XM_014070700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRB3"/>
    <dbReference type="GeneID" id="106552430"/>
    <dbReference type="KEGG" id="tsr:106552430"/>
    <dbReference type="OrthoDB" id="5950381at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990003">
        <property type="term" value="F:IDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990174">
        <property type="term" value="F:phosphodiesterase decapping endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016077">
        <property type="term" value="P:sno(s)RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054754">
        <property type="entry name" value="NudT16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31699">
        <property type="entry name" value="NUDIX T16 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31699:SF5">
        <property type="entry name" value="U8 SNORNA-DECAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22327">
        <property type="entry name" value="Nudt16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="17">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013926175.1"/>
        </source>
    </evidence>
    <sequence length="172" mass="19670" checksum="BEC749838472763B" modified="2020-10-07" version="1">MFMFLPGVKCYWFRPVRKDAMQMRFDGRLGFPGGFVDPRDISLEEGLNRELCEELGPGAASLHLAEKDYLSSHASEQPQRVVMHFYAKQLSLEEFRRVEEGAIQAKDHGFEVMGLIRVPLYILRDGVGGLPMFLSNTFIGNAREQLIHALETLQLMPAEQLQKAIRIPQKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS11</accession>
    <name>A0A6I9YS11_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of G-protein signaling 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926946.1">
        <property type="nucleotide sequence ID" value="XM_014071471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS11"/>
    <dbReference type="GeneID" id="106553043"/>
    <dbReference type="KEGG" id="tsr:106553043"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926946.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="17733" checksum="7B8C47746D112F28" modified="2020-10-07" version="1">MPNTARYSSPPRVTLGDASVTEKNLEPNRLGSDLIPSLLERAQNSMDSELDNCHSSSNAVNSKVSPKPKLALSLGRFLKWRCLNSPVFATLSPKHPAVVQGKVQPLEDLSQHLQPKSKKASIAFFQISLESKIHPMDPRVEEDHHPWPYKEFAKEVICP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS17</accession>
    <name>A0A6I9YS17_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ketimine reductase mu-crystallin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CRYM</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926729.1">
        <property type="nucleotide sequence ID" value="XM_014071254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS17"/>
    <dbReference type="GeneID" id="106552860"/>
    <dbReference type="KEGG" id="tsr:106552860"/>
    <dbReference type="CTD" id="1428"/>
    <dbReference type="OrthoDB" id="41492at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070324">
        <property type="term" value="F:thyroid hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042403">
        <property type="term" value="P:thyroid hormone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000241">
        <property type="entry name" value="ketimine reductase mu-crystallin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926729.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="25647" checksum="8DB8268898735284" modified="2020-10-07" version="1">MNSAPVFLDAGAVEKHWPASGALLSALEAALAAFSSGAEGGVVQPLRTVVPIQKHNGTSNVYAYLMPAVSEVLCILGAGVQAYSHYELFTQLFSFKEVRIWNRTKKNAEKFASKVNGPVRVCSSVQDAVNGADVIVTVTMATQPILHGEWVKPGAHINAVGASRPDWRELDDNVMSNSVLYVDSKEAALRESGDVILSGVRRSMVFIPQLSVIFESFSGMAIEDTITAKMVFEGWSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSD2</accession>
    <name>A0A6I9YSD2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein phosphatase 1 regulatory subunit 36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PPP1R36</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927458.1">
        <property type="nucleotide sequence ID" value="XM_014071983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSD2"/>
    <dbReference type="GeneID" id="106553477"/>
    <dbReference type="KEGG" id="tsr:106553477"/>
    <dbReference type="CTD" id="145376"/>
    <dbReference type="OrthoDB" id="6724830at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Pro_pase_1_reg_su_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21055">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21055:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14895">
        <property type="entry name" value="PPPI_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927458.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="49794" checksum="1FD32E75B1A56DCB" modified="2020-10-07" version="1">MSQIQFLELPKGVYLPLTIINPGLWYWKEDTRTLEFSCAQLKEEPKDSKEKNKKGKTIQFQELGAKPLERSGPNYFRKLFTLSVSSGEDKSPRPNKRALSELITLEDVKYAALFLAHEDDSIHISSFSLVMRCKQLDDFLMALLYYISFYLEKITLEMKPKSFMGKPSILEKKELKENQMKLAVTKKHLAEMYCILILGLGMSDQHHMACGKKKISSSRKDREFFECLYNFASYVAFVTFRQKNFKEIQQEVGRLLRSNIFNSALRERTKYSREMKTDITKQLLKATFAFRKRTDVKQPAIRSVMDQRSPVLSTLLPLAKDSAQYLFQDHYLRPSKTAPRYIPEELPELFSIVSPTKIGIIGSNRNKFVPHTLLPIGSTEDEKENGENLEESVSTVMSQDITSATSRGIQSGMSHQSLAVSRATTEGDYIENPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSJ7</accession>
    <name>A0A6I9YSJ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fibroblast growth factor receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106553189</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927111.1">
        <property type="nucleotide sequence ID" value="XM_014071636.1"/>
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    <dbReference type="GeneID" id="106553189"/>
    <dbReference type="KEGG" id="tsr:106553189"/>
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    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005007">
        <property type="term" value="F:fibroblast growth factor receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001927">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF131">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013927111.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="29183" checksum="B530835EA07C5ADD" modified="2020-10-07" version="1">MTPEDCNRSKYESVAKHPNFDHMTMGYAAMILSCIHRDLAARNVLVTEDIVMKIADFGLARDIHHIDYYKKTTNVSGISQRGLKLFHFQALCQVPNSIQNPACNCCSIRQDVSCMLTRWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVAMTSNQEYLDLSLPLDQYSLSFPDTRSSTCSSGEDSVFSHDPLPDEPCLPKHPPHLANGGLKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTF7</accession>
    <name>A0A6I9YTF7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transmembrane protein 119</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TMEM119</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927602.1">
        <property type="nucleotide sequence ID" value="XM_014072127.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106553587"/>
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    <dbReference type="OrthoDB" id="8943443at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030501">
        <property type="term" value="P:positive regulation of bone mineralization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033690">
        <property type="term" value="P:positive regulation of osteoblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031453">
        <property type="entry name" value="TMEM119"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28645">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28645:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15724">
        <property type="entry name" value="TMEM119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026712775" evidence="3">
        <location>
            <begin position="21"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013927602.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="32084" checksum="238913B86D78055E" modified="2020-10-07" version="1">MAAMSDLCLLLLLMLPFCASRSVQNLALEESEGSGDSEGAPPDPTTARAALSVTPTLGSLVVDSVNGTLHSFNLLDGIVNFFQTYMLLIVVVGSLLLIFLSIVCAAVITQQKHKASAYYPASFPKKKYVDQNDKAGGAKAFSEVPEKPSDESQEEPVDSTKQLQADILAAAQNLKSPTKAVMANGKSGPVEDPPVEGKEEGAPKTEEAEESSSKEEEEEEEEEGKSPTEAEMPPETPAPNSLADAETRKEEVPPGVETHPSPPEESKEAPPTEKPASEGQKEQKEELSAPLTLDPTEEPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTI4</accession>
    <name>A0A6I9YTI4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 116</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927627.1">
        <property type="nucleotide sequence ID" value="XM_014072152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTI4"/>
    <dbReference type="GeneID" id="106553616"/>
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    <dbReference type="CTD" id="89894"/>
    <dbReference type="OrthoDB" id="10070607at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022343">
        <property type="entry name" value="GCR1-cAMP_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02001">
        <property type="entry name" value="GCR1CAMPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="43"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013927627.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="41630" checksum="57C965B470D65178" modified="2020-10-07" version="1">MASFAPGAATQELLLPLQDLERAQLFAVIHWIQAVTATLSIMGSSSVIGYTVFQNTARSPEVRPLVYLSLSDLFLGLCWLTGALLYSREASHPTGEDNTCYNLQVTGQIFYVASFLYTVNYTWQLYTDLRGKFNQHLHSQMPQMTGYSNRLGRIAILFSSLVPIFLMAPVLGLGNHFQCPQNVTAEHGCLLLHTGNMLSTGSSQHVTCAVLCLYSTGVFLVSFMLSFLAILTLLIRARMLYKRFVNSMGYVGDQQWEMLKVVEQAVVLYPVVFFCCWGPAFILGIVKLARMDNASVYMALYVLEALTASSQGFLNCVVYGWTQHMFHCVKRKACRDVNTQTPLLRSQKRFYASTLPARSQAVAKPSSPATTTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUQ1</accession>
    <name>A0A6I9YUQ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DmX-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927746.1">
        <property type="nucleotide sequence ID" value="XM_014072271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUQ1"/>
    <dbReference type="GeneID" id="106553711"/>
    <dbReference type="KEGG" id="tsr:106553711"/>
    <dbReference type="OrthoDB" id="342131at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000254">
        <property type="entry name" value="dmX-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052208">
        <property type="entry name" value="DmX-like/RAVE_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950:SF13">
        <property type="entry name" value="DMX-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950">
        <property type="entry name" value="RABCONNECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927746.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="33817" checksum="CC2BCC88BF5FDDE8" modified="2020-10-07" version="1">MQRVSNLSTSSMPWLGSGQTSTGAGILIKRNLNNVKRMTSHPFHQYYLTGAQDGSVRMFEWTRPQQLICFRQAGNARVTRMYFNSQGNKCGVADGEGFLSIWQVNQASSNPKPYMSWQCHSKTTSDFAFITSSSLVATSGQSNDNRNVCLWDTLVSSSNNLIHAFTCHEHGATVLHYAPKHQLLISGGRKGHISIFDIRQRQALHTFHAHDSSIKALALDSSEECFCTGSAEGNVKVWRLTGYGLIHSFKHEHAKQSIFRNLSTGVMQIEMAPGNRIFSCGADGTLKMRVLPNAFNIPSDLCDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVL8</accession>
    <name>A0A6I9YVL8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arginine/serine-rich coiled-coil protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RSRC2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RSRC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927675.1">
        <property type="nucleotide sequence ID" value="XM_014072200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVL8"/>
    <dbReference type="GeneID" id="106553653"/>
    <dbReference type="KEGG" id="tsr:106553653"/>
    <dbReference type="CTD" id="65117"/>
    <dbReference type="OrthoDB" id="1928974at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028124">
        <property type="entry name" value="SMAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22426">
        <property type="entry name" value="ARGININE_SERINE-RICH COILED-COIL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22426:SF2">
        <property type="entry name" value="ARGININE_SERINE-RICH COILED-COIL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15477">
        <property type="entry name" value="SMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small acidic protein-like" evidence="4">
        <location>
            <begin position="335"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013927675.1"/>
        </source>
    </evidence>
    <sequence length="418" mass="48202" checksum="FD44713905789959" modified="2020-10-07" version="1">MAASDSEREANPPEKSSPEREKKKERSDASSSPRTSKHHYSRSRSRSRERKRKDNDGKKYRSRSRSKEARRHGSKEKSSKKHRPEEHNDKDHPSDKGRESLNSSSENGEERHKRKERKPSRGRSHSRSRSRERRHRSRSRDRKKSRSRSREKKRRVRSRSRSKSRHRHRSPSRSRSRSRSRERKKRPEKPRRFSRSHSRTPSPPPFRGRNTAMDAQEALARRLERAKKLQEQREKELVEKQKQQEMVAAAAATGGSVINVAALLASGTQVTPQIAMAAQMAALQAKALAETGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNFGNKDQNVKFRKLMGIKHEEEAGGAASSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSSMRGMDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWL0</accession>
    <name>A0A6I9YWL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PSMA5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928404.1">
        <property type="nucleotide sequence ID" value="XM_014072929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWL0"/>
    <dbReference type="GeneID" id="106554283"/>
    <dbReference type="KEGG" id="tsr:106554283"/>
    <dbReference type="CTD" id="5686"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03753">
        <property type="entry name" value="proteasome_alpha_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000019">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033812">
        <property type="entry name" value="Proteasome_alpha_type_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013928404.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="26455" checksum="0394C7ECCFEAF116" modified="2020-10-07" version="1">MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDSHIGCAMSGLIADAKTLIDKARVETQNHWFTYNEIMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGKKFHMYTKEELEDVIKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYC8</accession>
    <name>A0A6I9YYC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">T-box transcription factor TBX6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106554847</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929054.1">
        <property type="nucleotide sequence ID" value="XM_014073579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYC8"/>
    <dbReference type="GeneID" id="106554847"/>
    <dbReference type="KEGG" id="tsr:106554847"/>
    <dbReference type="OrthoDB" id="6831739at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF100">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="9">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="T-box" evidence="9">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="147"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929054.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="26695" checksum="8D1BE836BFFBB34C" modified="2020-10-07" version="1">MYHPSEIFPQYGSSYALRPPPPPGAAYPQSQAPQYEAFRYSELEAAPKLEGFFSGLDPPGRLLPPTPGLPYGSSQTLPTGPPTTAPLPPGVRMSLENSELWKRFSGIGTEMIITKAGRGTADRSFAVSRAAAQCKITQMKIESNPFAKGFRENGMNSKREREARIKRKMKTNECEANEGESESKKGPCDSTLTEEGPLEQQPPPALPQPSCSFYPPTGINLNGAEAYLQHPAAFHGLRGPASPYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYY6</accession>
    <name>A0A6I9YYY6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEAT repeat-containing protein 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106554602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928790.1">
        <property type="nucleotide sequence ID" value="XM_014073315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYY6"/>
    <dbReference type="GeneID" id="106554602"/>
    <dbReference type="KEGG" id="tsr:106554602"/>
    <dbReference type="OrthoDB" id="66533at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013928790.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="19734" checksum="F4FCC6B1FD5DB0DB" modified="2020-10-07" version="1">MAAEACMAGGDSAAAEPNPAWIESGLCLQRYLARLKALRLAPDPSQAPGGENASRRRPELHLLFDELMSETCVPGGAAPWGPKPEDICELIVQACKLVQLNQEHLVNKVCQLIHQLLNRFQVRKLNFFCHILVEHLILSEVLFQLYCPWGFHGKDTGVGCYFLLHWITFSQFFSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZ19</accession>
    <name>A0A6I9YZ19_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dickkopf-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106555047</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with LRP5 and LRP6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dickkopf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929289.1">
        <property type="nucleotide sequence ID" value="XM_014073814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZ19"/>
    <dbReference type="GeneID" id="106555047"/>
    <dbReference type="KEGG" id="tsr:106555047"/>
    <dbReference type="OrthoDB" id="6359792at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039706">
        <property type="term" value="F:co-receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23274">
        <property type="entry name" value="Dkk3_Cys2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23014">
        <property type="entry name" value="Dkk3_N_Cys1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.80.10:FF:000003">
        <property type="entry name" value="Dickkopf WNT-signaling pathway inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006796">
        <property type="entry name" value="Dickkopf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039863">
        <property type="entry name" value="DKK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047300">
        <property type="entry name" value="Dkk3_Cys2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047301">
        <property type="entry name" value="Dkk3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113:SF8">
        <property type="entry name" value="DICKKOPF-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113">
        <property type="entry name" value="DICKKOPF3-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04706">
        <property type="entry name" value="Dickkopf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027012746" description="Dickkopf-related protein 3" evidence="13">
        <location>
            <begin position="22"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf N-terminal cysteine-rich" evidence="14">
        <location>
            <begin position="145"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013929289.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="39578" checksum="776BB67493F78C58" modified="2020-10-07" version="1">MFLLGSIRLLFLPALLGMVFSAPASQSKEKEQPQEIVLSFPKDETSLNEMLQEVEELMEDTQYKLTNAVKEMEAEEEGSKKIMGIEFQKLPANYHNESYTDTKVGNKTIHRHQEIDKVTDNKTGSTSYSETIITSIQEDRKRNHECIIDEDCEIGKYCAFSDLEYNCHICKPQHAHCSRDMECCGQQLCVWGECMQAVSKGENGTICENQRDCNPGLCCAFTKDLLFPICTPFPGEREPCRNPSDGLLNLITWELEPDGALDRCPCAHGLTCQKQSYSSDSLCELSLNKTQSEDKKQLLADELSLLDFVTQDIPDEYEERLIKEVQKELEDGLADSGKLAMTSDFFGEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z040</accession>
    <name>A0A6I9Z040_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">COP9 signalosome complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GPS1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929932.1">
        <property type="nucleotide sequence ID" value="XM_014074457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z040"/>
    <dbReference type="GeneID" id="106555583"/>
    <dbReference type="CTD" id="2873"/>
    <dbReference type="OrthoDB" id="422427at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000002">
        <property type="entry name" value="COP9 signalosome complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048624">
        <property type="entry name" value="CSN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21151">
        <property type="entry name" value="CSN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="305"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929932.1"/>
        </source>
    </evidence>
    <sequence length="527" mass="59512" checksum="8449E283BE8D1EAF" modified="2020-10-07" version="1">MRDSSVPSSASSSVTDLYNTPRSSRSDLFLPGTSGDFSLSACLSACTLLYEGAVEPMQIDVDPQEDQQNAPDINYVVENPTLDLEQYAASYSGLMRIERLQFIADHCPQLRVEALKMALSFVQRTFNVDVYEEIHRKLTEATRELQNTPDAVPDGGIEPPPLDTAWVEATRKKALLKLEKLDTDLKNYKGNSIKESIRRGHDDLGDHYLDCGDLSNALKCYSRARDYCTSAKHVINMCLNVIKVSVYLQNWSHVLSYVSKAESTPEIAEQRGERDSQTQAILTKLKCAAGLAELAARKYKQAAKCFLLASFDHCDFPELLSPSNVAVYGGLCALATFDRQELQRNVISSSSFKLFLELEPQVRDIIFKFYESKYASCLKMLDEMKDNLLLDMYLAPHVRTLYTQIRNRALIQYFSPYVSADMRKMATAFNTTVAALEDELTQLILEGLINARIDSHSKILYARDVDQRSTTFEKSLLMGKEFQRRAKAMILRAAVLRNQIHVKIHREDEGREEGYARHLKEAAGNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1J6</accession>
    <name>A0A6I9Z1J6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nephrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NPHS1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929921.1">
        <property type="nucleotide sequence ID" value="XM_014074446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1J6"/>
    <dbReference type="GeneID" id="106555575"/>
    <dbReference type="KEGG" id="tsr:106555575"/>
    <dbReference type="CTD" id="4868"/>
    <dbReference type="OrthoDB" id="10028801at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF136">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027118179" evidence="8">
        <location>
            <begin position="25"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="988"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="238"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="335"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="435"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="562"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="758"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="854"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="873"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1054"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1058"/>
            <end position="1084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013929921.1"/>
        </source>
    </evidence>
    <sequence length="1173" mass="128530" checksum="CFC048F1F3B10993" modified="2020-10-07" version="1">MSYCGLLVPLIVYFILEVNKGCQAEMLQQVFRVEPENVTIPEGETAMLKCEVENPSGTVQWVKEGLLLGPNWNIPRFPRYSMTGDTSKGIYNLQVIKSQLEDDAFYECQVGPTEKSTGIISHRVQLTVLVPVKSPLFVEYEANSVVTWIAGMEYTLHCQVKDARPPAEISFCKADKELADVTSTIQPGSADKLFSTEAMLRFTPQSSDNKELLTCKAVNTVTLIPVIVSFTMNILFPPQLPVIKGYTGVVVKNKELKLTCISPSGNPLATLQWLKNDEVISRNWETDDTNQLSRSSLSLKITPEDNMAIVSCQALNQVLSLPLQSNIVLKVTFPPEQVKITGSSSTEENQEIFLTCSTSTSNPPVMLRWWLGWRELNATETAISEAEYGGMVTVSNLTYIAYREDNDLPLICEAFNEAIMYTKSASVTLRVKYPPQKIWLNVPPPETYFRAGVEIKLICFASGGNPLPRLDWYKVTCTLAGGVRVQLRQPQESGVSKPCLGKQDIKLVREGASSASSGNIISKELVLITSPSDNMATYRCNATGPPSTPPITASTSLLVQFPPLHATITATDEKVRRGQSVTLTCKSGSSNPPARLTWLQDGKRLVGTNLGQRKAEYGGFSASERVTLVVSSADHGKRVECYAYSSVLSEGVNTFYQLTVLFPPEFSPEQTHLVQAVEHGSAQLPLLVSASPPEITYRWSFLGEMILTEGSPRYHLRDGGSLEIWNVTRADAGKYRIHCENEEGQNETTITLNVHYSPSIRSIADPTYVDLGDAAEIVCQADANPLPNFQWRWLGDLERSLEDLGFKLLSEGLLATLQIQAAQRTHAGVYECQVDNGISPAAKSSARLIVRYPPEIIKGPGQRKVAASGDGRSQAALQCSAQGVPSVLFSWAKNGVSLDLQSPRYHWPGAPSFLYIDVFPPQSPTFTLTGLQPNTPYNVSVNARNALGESDFADGGAGLSITTEERIEIPVDKEPEIPFPSEPESSILPLPVVVLLGGLGGFLLVSNLFLLGCLVFRKRTQTPKGDGIEGSTRGKVRFGNSYTVSKWLKVGGQPAQLEGSSSEGSSTKTSAAVSTATTDSSVTDPNISLPWMGPQEHHEYEEVGGPRRLEDMGPLYDEWNPNDEPWINEYSHVYQGFGRRPAASTKTAPIYDSVANYLPLDTELPFEQQGELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1S3</accession>
    <name>A0A6I9Z1S3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Integrin beta-1-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ITGB1BP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930229.1">
        <property type="nucleotide sequence ID" value="XM_014074754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1S3"/>
    <dbReference type="GeneID" id="106555841"/>
    <dbReference type="KEGG" id="tsr:106555841"/>
    <dbReference type="CTD" id="26548"/>
    <dbReference type="OrthoDB" id="10261079at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06488">
        <property type="entry name" value="p23_melusin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1130.20:FF:000001">
        <property type="entry name" value="Cysteine and histidine-rich domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1130.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007051">
        <property type="entry name" value="CHORD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039790">
        <property type="entry name" value="CHRD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983:SF2">
        <property type="entry name" value="INTEGRIN SUBUNIT BETA 1 BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04968">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51401">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0401">Integrin</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CHORD" evidence="6">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CHORD" evidence="6">
        <location>
            <begin position="153"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="5">
        <location>
            <begin position="223"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="318"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="338"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930229.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="38845" checksum="FF05BFE7097DD709" modified="2020-10-07" version="1">MALLCYRKGCGQRFDPEKNSQDACLHHPGFPIFHDALKGWSCCKKRTTDFSEFLSIKGCTKGFHSNEKPPEPEDASAQPKPCTADLVPEPKSTEVLQRERPSSDEPKQPLLVKVAQALQQVLEKLNASTAPQPAPHGLANPGGSSETSIGTACRNAGCKATFQGEESKTESCQFHPGVPVFHEGMKYWSCCGIKTTDFAAFLEQKGCSRGKHTWVTRQEDKKLVSCRQDWHQTSSQVVVTLYARTPLPHLSSIKANRTGLDIRVAFEGEKFFQAQLDLWGVINVEKSFASLLPSKVEIALKKASPIAWARLEQPHSQAEALQPLSQEVATPGSSLEAPLEESDDSLGWSEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z250</accession>
    <name>A0A6I9Z250_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106556244</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930707.1">
        <property type="nucleotide sequence ID" value="XM_014075232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z250"/>
    <dbReference type="SMR" id="A0A6I9Z250"/>
    <dbReference type="GeneID" id="106556244"/>
    <dbReference type="KEGG" id="tsr:106556244"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000005">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="9"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013930707.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="13613" checksum="FA81E537B289A9D0" modified="2020-10-07" version="1">MAGGKAGKDSGKTKTKTVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2E2</accession>
    <name>A0A6I9Z2E2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger SWIM domain-containing protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106556767</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931228.1">
        <property type="nucleotide sequence ID" value="XM_014075753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2E2"/>
    <dbReference type="GeneID" id="106556767"/>
    <dbReference type="KEGG" id="tsr:106556767"/>
    <dbReference type="OrthoDB" id="10013584at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048370">
        <property type="entry name" value="ZSWIM4-8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22619">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN CONTAINING PROTEIN 4, 5, 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22619:SF4">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21055">
        <property type="entry name" value="ZSWIM4-8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZSWIM4-8 C-terminal" evidence="4">
        <location>
            <begin position="128"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013931228.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="35448" checksum="E0A36F10241F74FC" modified="2020-10-07" version="1">MRMTLNTMTWRRREMVRWLVSCATEIGVQALMNIMQNWYTLFTPIEATTIVAVTGTTHATLLRLSLGSGQRDELCSCARTLALQCAMKDPQNCALPALTLCEKNHAAFEAAYQIVLDSAASAGMGHSHLFTIARYMEHRGLPVRAYKLATLAFSHLNIAFNQDTHPALNDVLWACSLSQSLGKNELAAIVPLVIKSVQCAPMLSDILHRWSLPPPGLASMGGGRRGAGGTGTGGKLFAMDKAPLCQLLEAAMAAYISTTHSRLTHISPRHYGEFIEFLGKARETFLMAHEGHLRFSQFLDNLKQTYKGKKKLMLLVRERFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2P6</accession>
    <name>A0A6I9Z2P6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ankyrin repeat domain-containing protein SOWAHB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SOWAHB</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOWAH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930504.1">
        <property type="nucleotide sequence ID" value="XM_014075029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2P6"/>
    <dbReference type="GeneID" id="106556064"/>
    <dbReference type="KEGG" id="tsr:106556064"/>
    <dbReference type="CTD" id="345079"/>
    <dbReference type="OrthoDB" id="60433at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058889">
        <property type="entry name" value="WHD_SOWAHA-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491:SF3">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN SOWAHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25877">
        <property type="entry name" value="WHD_SOWAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SOWAHA-C winged helix-turn-helix" evidence="6">
        <location>
            <begin position="4"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="212"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="276"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930504.1"/>
        </source>
    </evidence>
    <sequence length="615" mass="66022" checksum="B9935487E20330F8" modified="2020-10-07" version="1">MAQELSQAELLDFLCRAGGRVANAALLGHFKRFLRDPRADPGQLQRRRHLFKGFVNSVATVSQDGPAAATYVVLKKRYRDLIGEEESEPPPRAPVGRSPPPQLQQAPKSPGAAAAAAAAALRETGRFSDGEVAAARRKAGRCPPASPGVGSRPSPPLGCLGRERKGPRPDAPDPPQSSNGLGLPIGEMGALPNGGSAQQRIRDWVVTHHAASMPSQPSLAGEEAFPVPAWSQSSDRSSCRPGGSLDWGSSEGWWEPPVPVFRSIRSRLSLEDLEGHLEQESSVSEEGSSASWGSGSHPRDGEADGSGIRNSAGHEGPGDAPHSNGLMVVMTPLVMPLVQDAKPPPHVALLHRIVSRATTMGKTESQDLLKTVRPPSPNLDAPLSPRLAPRQRSVANVPLAERLAPNSDGSAEHRSSLVPLDGREHTWLVKIATGSWMQVRALLQEDPHLVLHRDFVSGFTVLHWLAKHGNAQALQDFVAGAEEAGIVLDVNVRSGCGYTPLHLAAIHSHPLVMKILVQRLHCRTQVRDSSGKRAWQYLSANASGEIWQLLGGPRGRTIFPTHPIIRPAAVSSSSTSLARKVSRKPSLASYLKPQHTKRKIGCKCSVLQEIEEYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2R8</accession>
    <name>A0A6I9Z2R8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neural cell adhesion molecule L1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106556463</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930932.1">
        <property type="nucleotide sequence ID" value="XM_014075457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2R8"/>
    <dbReference type="GeneID" id="106556463"/>
    <dbReference type="KEGG" id="tsr:106556463"/>
    <dbReference type="OrthoDB" id="6244967at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026966">
        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Neurofascin/L1/NrCAM C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930932.1"/>
        </source>
    </evidence>
    <sequence length="85" mass="8834" checksum="C27F094A680B5DB6" modified="2020-10-07" version="1">MKDETFGEYSDNEEKPFTSSQPSLNGDIKALGSDDSLADYGGSVDVQFNEDGSFIGQYSGQKGKEGGGNDSSGATSPTNVVTALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3J2</accession>
    <name>A0A6I9Z3J2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106556002</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013930425.1">
        <property type="nucleotide sequence ID" value="XM_014074950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3J2"/>
    <dbReference type="GeneID" id="106556002"/>
    <dbReference type="KEGG" id="tsr:106556002"/>
    <dbReference type="OrthoDB" id="8815311at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09931">
        <property type="entry name" value="SH2_C-SH2_SHP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10340">
        <property type="entry name" value="SH2_N-SH2_SHP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000012">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000018">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46559:SF7">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46559">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="6"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="112"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013930425.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="34914" checksum="3FC2C55E8E7040D3" modified="2020-10-07" version="1">MISRRWFHPNISGIEAEKLLLTRGIHGSFLARPSKSNPGDFTLSVRRNDEVTHIKIQNTGDYYDLYGGEKFATLAELVQYYTEQEGLLRERNSNTIELRFPLNCQDPTSERWYHGHLTGKEAEKLLTDKAKPGSFLVRSSQSKPGDFVLSVLTNEDKADTGDRKPRVTHIMIRYQLDGKYDVGGGERFDTLADLVEHYKKNPMVEKSGVVVHLKQPFNATRIIAANIENRVKELNKMADQSEKAKQGFWEEFEMLQQQECKFLYPRKEGQQAENKAKNRYKNILPCKNSESGTILKVGNILSLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z405</accession>
    <name>A0A6I9Z405_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sodium/potassium/calcium exchanger 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106556491</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930959.1">
        <property type="nucleotide sequence ID" value="XM_014075484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z405"/>
    <dbReference type="GeneID" id="106556491"/>
    <dbReference type="KEGG" id="tsr:106556491"/>
    <dbReference type="OrthoDB" id="2127281at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF42">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="136"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013930959.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="23011" checksum="27DD78291A9525BE" modified="2020-10-07" version="1">MYFIYILIMKFNANLRHMFERRTKNVTNMVNGLANNTEMDDSSNCDATVVLLKKGSRNFHHKASVIMVDELLSAYPHQLSFSEAGLRIMITSHFPPRTRLSMASRMLINERQRLINSRVYTNGESEIAIKVPFKHTMENGSGLNNSAKGSTNGTRRDEEAAAGGNETENENDDNENNENDEEEEDDDDDDDDDQGPYTPFDPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5B0</accession>
    <name>A0A6I9Z5B0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">DNA polymerase</fullName>
            <ecNumber evidence="26">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">POLB</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="26">DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.</text>
    </comment>
    <comment type="function">
        <text evidence="23">Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24 26">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 5'-end 2'-deoxyribose-2'-deoxyribonucleotide-DNA = (2E,4S)-4-hydroxypenten-2-al-5-phosphate + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76255"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:18657"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:195194"/>
            <dbReference type="ChEBI" id="CHEBI:195195"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 26">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 26">Belongs to the DNA polymerase type-X family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="26"/>
    <dbReference type="RefSeq" id="XP_013931394.1">
        <property type="nucleotide sequence ID" value="XM_014075919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5B0"/>
    <dbReference type="GeneID" id="106556881"/>
    <dbReference type="KEGG" id="tsr:106556881"/>
    <dbReference type="CTD" id="5423"/>
    <dbReference type="OrthoDB" id="205514at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000002">
        <property type="entry name" value="DNA polymerase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000026">
        <property type="entry name" value="DNA polymerase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.210.10:FF:000008">
        <property type="entry name" value="DNA polymerase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000021">
        <property type="entry name" value="DNA polymerase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019843">
        <property type="entry name" value="DNA_pol-X_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028207">
        <property type="entry name" value="DNA_pol_B_palm_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018944">
        <property type="entry name" value="DNA_pol_lambd_fingers_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002008">
        <property type="entry name" value="DNA_pol_X_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF42">
        <property type="entry name" value="DNA POLYMERASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14792">
        <property type="entry name" value="DNA_pol_B_palm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10391">
        <property type="entry name" value="DNA_pol_lambd_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00869">
        <property type="entry name" value="DNAPOLX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00870">
        <property type="entry name" value="DNAPOLXBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81585">
        <property type="entry name" value="PsbU/PolX domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00522">
        <property type="entry name" value="DNA_POLYMERASE_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="27" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 26" id="KW-0227">DNA damage</keyword>
    <keyword evidence="18 26" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15 26" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="19" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="9 26" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20 26" id="KW-0539">Nucleus</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 26" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase X" evidence="29">
        <location>
            <begin position="42"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="28">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="28">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; Schiff-base intermediate with DNA; for 5'-dRP lyase activity" evidence="25">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00044678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR622312-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU366014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="SMART" id="SM00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013931394.1"/>
        </source>
    </evidence>
    <sequence length="363" mass="41220" checksum="437DF138A7EABAD1" modified="2020-10-07" version="1">MPKLVLGGRVRVPAANGPAHAHDVTCRAAAWDSHFVRQARVRSQRDLEELANYEKNVNRAIHKYNAYRKAASVISKYPSKIKSGSEAKKLDGVGTKIAEKIDEFLSTGKLRKIEKIRQDDTSSSINLLTRVSGIGPAAARKLVDEGIKTLEDLKKNENKLNHHQRIGLKYFKDFEKRIPRKEMLEMQDIVLNEVKKVDPAYIASVCGSFRRGAESSGDMDILLTHPDFTSESAKKPKLLHQVVEQLESIRFVTDTLSKGETKFMGVCQLPGKDDDTVYPYRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRTHALEMGFTLNEYTIRPLGITGVAGEPLPVDSEKDIFDYIQWPYREPKERSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WY61</accession>
    <name>A0A6I9WY61_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Spectrin beta chain, non-erythrocytic 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106538029</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907874.1">
        <property type="nucleotide sequence ID" value="XM_014052399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WY61"/>
    <dbReference type="GeneID" id="106538029"/>
    <dbReference type="KEGG" id="tsr:106538029"/>
    <dbReference type="OrthoDB" id="5865767at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0117">Actin capping</keyword>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="202"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="315"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013907874.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="41109" checksum="B8A438E92C900367" modified="2020-10-07" version="1">MDWLQIVMDVLMFGRDASMAEAWLASQEPIVRSTELGDNVDEVENLIKRHEGFQKSVAAWEERILALEKLTTLEEKEHREQEEARKRTSPAPTPLEPTVMIPQTDNTDGHHLPSTTQTNGSPGQQGLTLELPSQNGLDPDSESPQISESSSAVNGSQLDSVSKGKEPSPPASPKLSSKPAVFSTDPAQSVTLPPRTPDDSASGQMGGILHRKQEMESHGKKAANRSWQTIYCVVQKNSFGFYKDSKHATNSIPYHGEAPVSLQGAQCNVALDYKKRKHVFKLGLTDGKEYLFQAKDEAEMSTWMRVINAAAASALTPQDPAEDQPALISKGMTRAMTMPAGPPHSASEGTVTLRSKEAKEKDREKRFSFFKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZF5</accession>
    <name>A0A6I9WZF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Dexamethasone-induced Ras-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RASD1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907511.1">
        <property type="nucleotide sequence ID" value="XM_014052036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZF5"/>
    <dbReference type="GeneID" id="106537791"/>
    <dbReference type="KEGG" id="tsr:106537791"/>
    <dbReference type="CTD" id="51655"/>
    <dbReference type="OrthoDB" id="265044at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04143">
        <property type="entry name" value="Rhes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000475">
        <property type="entry name" value="GTP-binding protein Rhes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF4">
        <property type="entry name" value="DEXAMETHASONE-INDUCED RAS-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013907511.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="31765" checksum="3CEC58C4FD37C93F" modified="2020-10-07" version="1">MRLAEMIKKMGPCEVELNIPPKNCYRMVVLGSSKVGKTAIVSRFLSGHFEDQYTPTIEDFHRKLYSIRGEVYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRDSFEEVQRLKQQILETKSCLKNKTKENIEVPLVICGNKGDRDFYREVESREIEQLLGQDSKKCPYFEISAKKNSSLDEMFQALFTMAKLPNEMSPDLHRKVSVQYCDLLHKKTLKGKKLLKDSSGDGTGEAYGIVAPFARRPSVHSDLMYIREKAIRGSQAKDKERCVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0Q9</accession>
    <name>A0A6I9X0Q9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="18">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">B4GALT6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17950"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:31496"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Golgi apparatus membrane</location>
            <topology evidence="18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_013907372.1">
        <property type="nucleotide sequence ID" value="XM_014051897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0Q9"/>
    <dbReference type="GeneID" id="106537705"/>
    <dbReference type="KEGG" id="tsr:106537705"/>
    <dbReference type="CTD" id="9331"/>
    <dbReference type="OrthoDB" id="10038994at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008489">
        <property type="term" value="F:UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006688">
        <property type="term" value="P:glycosphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000037">
        <property type="entry name" value="Beta-1,4-galactosyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF47">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="108"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="247"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013907372.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="44513" checksum="E9A67E64E223678F" modified="2020-10-07" version="1">MPLFRTALRVSNRSILVFIFFFSFSSSCLYFIYVAPGMANTYLFMVQARGIMLKENVKTIGHMIRLYTNKNATINGTDYPEGNSTSDSIAQTVMYLPENFTYSPSQSCPEKLPYMRGFIEANMSEISLEEIQQMFSKDLDIEPGGHWKPKDCKPRWKVAILIPFRNRHEHLPIFFRHLIPVLQKQRLEFAFYVIEQIGTQPFNRAMLFNIGFKEAMKDSDWDCVIFHDVDHLPENDRNYYGCGEMPRHFAAKLDKYMYILPYNEFFGGVSGLTVEQFKKINGFPNAFWGWGGEDDDLWNRVHSVGFNVTRPEGELGKYKSIPHHHRGEVQFLGRYKLLRYSRERQFIDGLNNLAYIPSIFANKLYTNITVNLVPELAPVQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1A5</accession>
    <name>A0A6I9X1A5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nanos homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NANOS2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the nanos family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908121.1">
        <property type="nucleotide sequence ID" value="XM_014052646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1A5"/>
    <dbReference type="GeneID" id="106538210"/>
    <dbReference type="KEGG" id="tsr:106538210"/>
    <dbReference type="CTD" id="339345"/>
    <dbReference type="OrthoDB" id="5864971at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.30:FF:000001">
        <property type="entry name" value="nanos homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.30">
        <property type="entry name" value="Nanos, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008705">
        <property type="entry name" value="Nanos/Xcar2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038129">
        <property type="entry name" value="Nanos_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024161">
        <property type="entry name" value="Znf_nanos-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12887">
        <property type="entry name" value="NANOS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05741">
        <property type="entry name" value="zf-nanos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51522">
        <property type="entry name" value="ZF_NANOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nanos-type" evidence="10">
        <location>
            <begin position="95"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013908121.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="18786" checksum="C0093687212388C2" modified="2020-10-07" version="1">MFSASSQSSPAGVPAVAQGGFFEPWKDYLNLSKVVMEIVEERKKPQRWSPPWEDPLDVLVLHWMNGGGNSKTHSPKTDAARGGDPPPAPPTQQGICSFCKHNGESKKVFSSHALKRADGVVVCPILRNYTCPLCGATADKAHTLKYCPHNKGQQSLYRRSGRNSAGRMIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1M6</accession>
    <name>A0A6I9X1M6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Grainyhead-like protein 3 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GRHL3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907727.1">
        <property type="nucleotide sequence ID" value="XM_014052252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1M6"/>
    <dbReference type="GeneID" id="106537947"/>
    <dbReference type="KEGG" id="tsr:106537947"/>
    <dbReference type="CTD" id="57822"/>
    <dbReference type="OrthoDB" id="7680836at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF6">
        <property type="entry name" value="GRAINYHEAD-LIKE PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25416">
        <property type="entry name" value="GRHL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="7">
        <location>
            <begin position="224"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013907727.1"/>
        </source>
    </evidence>
    <sequence length="600" mass="67966" checksum="CF6E35874337A900" modified="2020-10-07" version="1">MSNDVDFMSVRLLKNDGMGLQKYSYMNEDEAWKSYLENPLTAATKAMMRVNGDDDSVAALSLLYDYYMVPKEKRILPSGTQGRSEVGKRHCHGMEYEPEISSFEGSAQLMKLLAENVSVNQEYLDLPKKNGLAFEGLSSPHKPPALPPGPSKLESNPENYMITSGDMYDNGSLNSLFDSIHVAPPQQRWQPDSTFKEDPEESLIFNDILKAEAPCSEGYSISDGKTDFEYTLGSPKAIHIKAGDSPMAYLNKGQFYPVTLRTVGDAKCLHLSSNKVKSVVMVVFDNEKIPMEQLKFWKHWHSRQPTAKQRVIDVADCKENFNTVQHIEEVAYNALSFVWNIAEEAKVFIGVNCLSTDFSSQKGVKGVPLNLQIDTYDYSTGTNRLVHRAVCQIKIFCDKGAERKMRDDERKQFRRKGKCLDANNNGLKSCLVSSFRSNEITYLKPQSDLDTQPVLFIPNVHFSNLQRCTTVLPTAGIPHDSHSLPLKRSCPSFPDEFDPIAAKQAKEEDPQRVLLYVRRETEEVFDALMLKTPDLKGLRNAISEKYGLPEESIYKVYKKCKRGILVNMDNNIIQHYSNHMAFLLDVVEAEEKFRVTLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1P8</accession>
    <name>A0A6I9X1P8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-box only protein 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">FBXO30</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with SKP1, CUL1 and RBX1/ROC1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909178.1">
        <property type="nucleotide sequence ID" value="XM_014053703.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909179.1">
        <property type="nucleotide sequence ID" value="XM_014053704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1P8"/>
    <dbReference type="GeneID" id="106539035"/>
    <dbReference type="KEGG" id="tsr:106539035"/>
    <dbReference type="CTD" id="84085"/>
    <dbReference type="OrthoDB" id="5918172at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22174">
        <property type="entry name" value="F-box_FBXO30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.150:FF:000001">
        <property type="entry name" value="F-box only protein 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.150">
        <property type="entry name" value="TRAF-like zinc-finger, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031890">
        <property type="entry name" value="Fbxo30/Fbxo40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043013">
        <property type="entry name" value="Znf_TRAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15933:SF13">
        <property type="entry name" value="F-BOX ONLY PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15933">
        <property type="entry name" value="PROTEIN CBG16327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15966">
        <property type="entry name" value="F-box_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15965">
        <property type="entry name" value="zf-TRAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="11">
        <location>
            <begin position="63"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="12">
        <location>
            <begin position="626"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="10">
        <location>
            <begin position="63"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909179.1"/>
        </source>
    </evidence>
    <sequence length="761" mass="84594" checksum="C3D59A50A4B2C1FB" modified="2020-10-07" version="1">MQAMSSSEEKTEPATMEDLQHLHCVNCISRRCMTRSEAGISCDLIGCPMVCGAVFHLCKAEEHRLLCPLERVPCLNSGFGCPFTVTRNKIAEHLETCPASVVCCTMEWNRWPISYADRKSYENLSKDVDEIEQLDMALALQDQRMLLESLKVATMTSKTPDPILKPREQSSVASAPDAAVANGLLPVDEESFGALYQATVETTKSLATALDILNTATSLLSSKLSILPCKRNADCCVKEHSSMQAMVEHDSEEENSMGAVGGIKLDILNQNGQLEQNGSSDICYDTTKHYKLNMSLDNIPLCNGFHLDDSSTEKLPQNEELNICNSSQFNVANSIHSASPCDKAANVCNSVPVAQVKYEIPLHSLSNGTTDHIYMSHDCNDEQLQREIEQEKLRNNVFSFIPQHTYNYLANYCLSASKKDKAVDTSDLEITEDPMDLQGIDLITAALLFCLGDSPGGRGISDCRIIDGYHVDFGTQTFSLPSAILATNTMVGEIASASACDHANPQLSNPSPFQTLGLDLVLECVTRYQTKRSMFTFVCGQLFRRNEFSSHFKNVHGDIHAGLNGWMEQRCPLAYYGCTYSQRRFCPSTQGAKIIHDRHLRSFGVQPSISTVLSEPDKRCCLGLHSDNLSNLPFEVLQHIAGFLDGFSLCQLSQVSRLMRDVCGSLLQARGMVILLWEKRIYSDGSFSWQIKEKVWRFSTAFCTVNEWKFADIVSMADHLKKCSYNAVEKREEAVPLPCMCVTRELTKEGRSLRSVLKPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2J4</accession>
    <name>A0A6I9X2J4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Programmed cell death protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PDCD2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908546.1">
        <property type="nucleotide sequence ID" value="XM_014053071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2J4"/>
    <dbReference type="GeneID" id="106538561"/>
    <dbReference type="KEGG" id="tsr:106538561"/>
    <dbReference type="CTD" id="5134"/>
    <dbReference type="OrthoDB" id="443682at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298">
        <property type="entry name" value="PCDC2 PROGRAMMED CELL DEATH PROTEIN 2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298:SF4">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="139"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013908546.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="39839" checksum="9065B530382BA853" modified="2020-10-07" version="1">MCSHQVELGFVEEAAPWRLRSDQFPCKVGGRPAWLGEAGLPGPSELRCGVCGQPCAFLLQLYAPVQERPEAFHRSLFLFGCRQPSCYRPAGPAPGSLKVFRNQLPRKNDTYSYNPPPEEPPLEELPSVNLQLKCGAMLCRVCGCLGPSTCSKCHKAHYCSKDHQIKDWKAGHKVSCLQPEDVIPDHKFLFPEYEIVREHEELDSVAVNDTEQEDLEKNDDTTDEASELLEEMSLEMMAKHETLEDNFFQIFKERISLEPEQIIRYCRNGEGPIWISGLNIPQDTDIPNCQCGSKRVFEFQVMPQLLAHLNVDSLGESIDWGTLAIYTCTVNCDLGNHYAEEFIWKQDISNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2Y2</accession>
    <name>A0A6I9X2Y2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CD99 antigen-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106538272</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD99 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908197.1">
        <property type="nucleotide sequence ID" value="XM_014052722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2Y2"/>
    <dbReference type="GeneID" id="106538272"/>
    <dbReference type="KEGG" id="tsr:106538272"/>
    <dbReference type="OrthoDB" id="8963727at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034109">
        <property type="term" value="P:homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000391">
        <property type="term" value="P:positive regulation of neutrophil extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072683">
        <property type="term" value="P:T cell extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022078">
        <property type="entry name" value="CD99L2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076:SF15">
        <property type="entry name" value="CD99 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076">
        <property type="entry name" value="CD99/MIC2 PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12301">
        <property type="entry name" value="CD99L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026727802" evidence="9">
        <location>
            <begin position="22"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013908197.1"/>
        </source>
    </evidence>
    <sequence length="185" mass="19199" checksum="6F954C4C8E007254" modified="2020-10-07" version="1">MGRLALSLPLALLLLAALCRGDDLFDLKDALGTEAPTKRPSAVTKKTDDGFNLEDAVFGDDDSPKPTAPPPQPKPGSHGHSGGFEDSDLFDGDLPPDNPKSGHPSNPSSGRDDSGSAGAKTDSPGMLAGIISSVVVAIAGALSSFIAYQKKKLCFKQSDQENIPVESQQGAHAAPAVQRTLLQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3R5</accession>
    <name>A0A6I9X3R5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106538841</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908941.1">
        <property type="nucleotide sequence ID" value="XM_014053466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3R5"/>
    <dbReference type="GeneID" id="106538841"/>
    <dbReference type="KEGG" id="tsr:106538841"/>
    <dbReference type="OrthoDB" id="9025240at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000078">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000044">
        <property type="entry name" value="Histone H3.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="11 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="44"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013908941.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="16228" checksum="D7CC728AD1E5C8CE" modified="2020-10-07" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRFIYLFRVTIMPKDIQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6Q6</accession>
    <name>A0A6I9X6Q6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein KRI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">KRI1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909941.1">
        <property type="nucleotide sequence ID" value="XM_014054466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6Q6"/>
    <dbReference type="GeneID" id="106539641"/>
    <dbReference type="KEGG" id="tsr:106539641"/>
    <dbReference type="CTD" id="65095"/>
    <dbReference type="OrthoDB" id="10252032at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="4">
        <location>
            <begin position="492"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="621"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013909941.1"/>
        </source>
    </evidence>
    <sequence length="713" mass="83746" checksum="55BE886A1C08CC6C" modified="2020-10-07" version="1">MPGQPALRVNAAFAARYGKYRRREELQRLQDKYGEEDEASSSESASSEEEELDPKLERDFYSMLGLLKTKDPRIYQKNVTFYTEEDSCSATVSKKTHIKKKEKPMLLKDYERKVMVEKAGKYEDEEEEEITVKKPLTYIEEQKELKEGFRKFVLESDEEEGDNSSALLQKRVKSKEEKEHEEADYISWLKGRKKLEAENELQDLIPLKTYWNDPKLDEGEQFLRDYILNQGYKEGSSEEEEEEEEEHQPKGLPGIQLAVSDSSDEGEFFLKKQEDFERKYNFRFEEPEAQQVKTYPRIIATSVRRKDEHRKEKRQKTQERKIKEREQKQQELKHLKNLKRQEILERLEKLHKITGRAGNSFQESDLEGDFDPHLHDQLMEVSLLSFILCQSASMQGVQEEGKPNELCSVSSEWNWDKWIGRDEGMWQQEEDHQPHCEDPDFVMDANYDPSQQPTHSKRKKQLEVPLLGKKKSRSPFAEAVSQGKKPFDPETKTFEQYLDEFYHLEYEDLIGDLPCRFKYRTVVPCNYGLSTEEILAADDKELNRWCSLRKTCMYRSEKEELQEKAVYEKKSQDAWKKKRVLKSFQTGMEEEKEGEQFQPKKKKKSKETKCFGNSSKETQLVSASCEEEGRLFPKTTTDSVTGEELDAPASDGSPSLPPRIQRSKKRGRRGLVLGTKIRMKGSAFSCQRLQAYGLNPKALRYRQLLREKRRKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6T3</accession>
    <name>A0A6I9X6T3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Centromere/kinetochore protein zw10 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC106540537</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="21">Interacts with NBAS and KNTC1/ROD; the interactions are mutually exclusive and indicative for its association in two different vesicle tethering complexes. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Component of the NRZ complex composed of NBAS, ZW10 and RINT1/TIP20L; NRZ associates with SNAREs STX18, USE1L, BNIP1/SEC20L and SEC22B (the assembly has been described as syntaxin 18 complex). Interacts directly with RINT1/TIP20L bound to BNIP1/SEC20L. Interacts with C19orf25 and ZWINT. Interacts with ZFYVE1. Interacts with RAB18 and this interaction is enhanced in the presence of ZFYVE1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZW10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911169.1">
        <property type="nucleotide sequence ID" value="XM_014055694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6T3"/>
    <dbReference type="GeneID" id="106540537"/>
    <dbReference type="KEGG" id="tsr:106540537"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.150:FF:000001">
        <property type="entry name" value="centromere/kinetochore protein zw10 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048343">
        <property type="entry name" value="ZW10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055148">
        <property type="entry name" value="ZW10_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20666">
        <property type="entry name" value="ZW10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22766">
        <property type="entry name" value="ZW10_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="20" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Centromere/kinetochore protein zw10 C-terminal" evidence="23">
        <location>
            <begin position="64"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="ZW10 C-terminal helical" evidence="24">
        <location>
            <begin position="212"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00069312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF20666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF22766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013911169.1"/>
        </source>
    </evidence>
    <sequence length="367" mass="41447" checksum="C683D5B22F2A7A14" modified="2020-10-07" version="1">MAARNLMTSEIHNTVKVSPDSLPALPALPASGAGDQPKVPEGSELLLKQVTNLENKTKLSRHTFSFPTCRISDSVQRLMALAYQTLQEASASTDQCCIQLFYLVRNIFHIFCDVVPMYHKENLQKLPQLAALHHNNCMYIAHHLLTMGHQFRSRLSDGTATFVDLVPGFRKLGTKCFLAQMRVQKDELLERLSISRNFSNLDDEENYSAANRAVRQVLHQLKRLGKIWQDVLPVNIYCRAMGTLLNTALVEIISRVLALEDISAENADRLHALCQTVVDEGPRVFVPLPEEKENRPFQEEVPVYVPKWMMFQELMLLLQASLQEIVDRWAGSKGPLAAEFSPSEVKNLIRALFQNTERRAAALASIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7J9</accession>
    <name>A0A6I9X7J9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106537686</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907353.1">
        <property type="nucleotide sequence ID" value="XM_014051878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7J9"/>
    <dbReference type="GeneID" id="106537686"/>
    <dbReference type="KEGG" id="tsr:106537686"/>
    <dbReference type="OrthoDB" id="6145535at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15232">
        <property type="entry name" value="7tmA_OR13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="5"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013907353.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="31288" checksum="1D40CCF37E496728" modified="2020-10-07" version="1">MALIANFILILTIYSYRKLHTPMYFLIVNLSLVNVFSISVTIPKLLHNLLTGRKTISFYGCIVQVYLFIWALGTELLLLSFMAFDRYAAICHPLQYSVIMKKEVCLGIASIVWIFGMINSGIHAGLMLKLSFCNSNIINHFFCDIPPLLQLSCSDTHPNETMAFISDVIFGICSCGLTFTSYFFILKTISSLHSTEGKKKAFSTCSSHFIIVSIFFSSVIYTYIRPSSVYALDQDKLISLLYSVVTPIVNPVIYSLRNKDFIEGFKTLIRRIRLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8G0</accession>
    <name>A0A6I9X8G0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rhomboid protease</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PARL</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013907613.1">
        <property type="nucleotide sequence ID" value="XM_014052138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8G0"/>
    <dbReference type="GeneID" id="106537860"/>
    <dbReference type="KEGG" id="tsr:106537860"/>
    <dbReference type="CTD" id="55486"/>
    <dbReference type="OrthoDB" id="10260614at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000005">
        <property type="entry name" value="Presenilins-associated rhomboid-like protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="13">
        <location>
            <begin position="181"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013907613.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="38982" checksum="D2043F64567BC5FD" modified="2020-10-07" version="1">MGWRCCTRVWVKQGLWGSGPWARRSILFTHPKNGFRRPALKTGTKLQKSSLTPPVKDLDFHESSRSLKKLVKPFFFTIGFSGCAFGTAAIWQYEHLRTKFQNYFENAQADWLDKIQPQKGLHFRKQINLWWNSLSNGQRAVSGIIAANVLVFCLWRIPALSRIMVTYFTSNPASRVVCFPMLLSTFSHFSFLHMAANMYVLWSFSTSIVSILGREQFMAVYLSAGVVSTFVSYVAKMATGKFGPSLGASGAIMAVLGAVCTKIPEAKLAIIFLPMFTFTAGNALKAIIALDSVGLILGWRFFDHASHLGGVLFGIWYVMYGNKLIWNNREPVVKAWHTVKTWRPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8V8</accession>
    <name>A0A6I9X8V8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cytosolic carboxypeptidase 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106537930</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:60004"/>
            <dbReference type="Rhea" id="RHEA-COMP:15519"/>
            <dbReference type="Rhea" id="RHEA-COMP:15675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:143623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:60005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907708.1">
        <property type="nucleotide sequence ID" value="XM_014052233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8V8"/>
    <dbReference type="GeneID" id="106537930"/>
    <dbReference type="KEGG" id="tsr:106537930"/>
    <dbReference type="OrthoDB" id="10253041at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06907">
        <property type="entry name" value="M14_AGBL2-3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000011">
        <property type="entry name" value="cytosolic carboxypeptidase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF23">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="282"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="576"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="628"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="998"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1032"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="635"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1042"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013907708.1"/>
        </source>
    </evidence>
    <sequence length="1054" mass="121572" checksum="D47163FFBF797C1F" modified="2020-10-07" version="1">MQDEDSFIPYGAEDLEQCAFVADSFCDQLLPRTTQILLEYNSGKWVPRLREPRDLYGLSSAGDLHLVRWPHQYEVIREKIKHIEWIPSHPEPLYNPTGLEIEPPCPDPGEGAVVYLSDEANKDPNFRYSRIGGSFPSKQVLPQTWNDWDNTLIFEARFESGNLQKVVKINDFEYQLTLRTDLYTNKHTQWYYFQVTNTQAGMPYRFTIVNFTKPTSLYNRGMRPLLYSEAEAKIHKVGWQRTGDQIKYYKNNFSNDGHQYFSLTWTFQFPHDRDTCYFAHCYPYTYSNLQDYLSAIARDPRRSKFCKIRILCHSLARNIVYVLTITNPLKDFNREKCKSAVILTARVHPGETNSSWMMKGFLDYLLGDSDNAQLLRDTFIFKVVPMLNPDGVIVGNYRCSLAGKDLNRNYRSELKESFPSIWYTRSMIKRIMEERNVLLYCDLHGHSRKENVFMYGCEKKEQDERACVLQRIFPFLMSKNCPDKFSFQDCNFKMQKGKEGTGRVVVWKMGVNNSYTLEVTFCGSKLGNRNATHFSAKDLESIGYNFCDSLLAFCTEKKSKYNECLKELEELVKQDDPAASLNDIGDRGDSSSDSSDSNEFTDKTEGQTKSKKKYLKVKKERSFVLESSGRRYSKHLHEPVNESKKPEDLSHRVSKTQEQIIKNAQVFQKLKLMELQNIEREKYEITKNVESFSRTTNAKKSKTLAGVHELFKETSSILKSKPDQMAFPSKKDAIPNVPSKYTLQYLSHYFPEQGLNLGWDLTHPFKHVVSQRLFLPPTAHNQMCNTPRKNSALLGCRFPRVENVKKEVSVHQKTSLIQMDSHPSSLYLSQPEEENHSEGGCVTLQLEEKELQNIALAQEIHLNAPYNKPVVWRLEKYQTGKTNSCREKTSPGIKGFLEAQKSILPGSKEKVKELSPARNAKTNKIGGGNCEGGLKLNRSSINQDLLLSLPLMSEEKYSQPTEKYLRSERHLKSLGRHRPRLATLLTTKGSNKTILVKHSEEDETSSPLLQSRPKMPPRNLLCQSENKFFPKRCKSDRPSVQDLPNTTERISFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9B6</accession>
    <name>A0A6I9X9B6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Eyes absent homolog</fullName>
            <ecNumber evidence="15">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">EYA2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 19">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14 15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the HAD-like hydrolase superfamily. EYA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013912194.1">
        <property type="nucleotide sequence ID" value="XM_014056719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9B6"/>
    <dbReference type="GeneID" id="106541321"/>
    <dbReference type="KEGG" id="tsr:106541321"/>
    <dbReference type="CTD" id="2139"/>
    <dbReference type="OrthoDB" id="167668at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001240">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02601">
        <property type="entry name" value="HAD_Eya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12350:FF:000001">
        <property type="entry name" value="Eyes absent homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028472">
        <property type="entry name" value="EYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006545">
        <property type="entry name" value="EYA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042577">
        <property type="entry name" value="EYA_dom_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038102">
        <property type="entry name" value="EYA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01658">
        <property type="entry name" value="EYA-cons_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10190">
        <property type="entry name" value="EYES ABSENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10190:SF7">
        <property type="entry name" value="EYES ABSENT HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 15" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="200"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR628472-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR628472-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013912194.1"/>
        </source>
    </evidence>
    <sequence length="534" mass="59123" checksum="0D856E1B8E09EE9A" modified="2020-10-07" version="1">MLDLVTPPSLTVNKEYPDILKLNLSNTYATVPDDIEGNRKSAPQHSFHLYTTKHYPHIVTVPSFATMATYGQTQYSAGIQQTAAYTAYPPPGQPYGIPSYSIKTEDSLSHSPGQSGFLSYGSSFSTATSGQTPYAYQMHGTSGIYQGPNGLTGSAGFSTGLHQEYSSYPSFTQSQYSQYYNSSYNSSYMSANNISPSTIPTSPYSVQESSHNVSGQSTESLPGEYTTPVKGIESDRQHRGTDGKLRGRSKRNSDPSPAGDTDIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHIDDVSSDDNGQDLSTYNFSADGFHSSNTGANLCLGSGVHGGVDWMRKLAFRYRRVKELYNTYKNNVGGLIGAPKRESWLQLRAELEALTDLWLTHALKALDLIHSRPNCVNVLVTTTQLIPALAKVLLYGLGVVFPIENIYSATKTGKESCFERIMQRFGRKAVYIVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9X7</accession>
    <name>A0A6I9X9X7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Differentially expressed in FDCP 6 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">DEF6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908063.1">
        <property type="nucleotide sequence ID" value="XM_014052588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9X7"/>
    <dbReference type="GeneID" id="106538165"/>
    <dbReference type="KEGG" id="tsr:106538165"/>
    <dbReference type="CTD" id="50619"/>
    <dbReference type="OrthoDB" id="8434295at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13273">
        <property type="entry name" value="PH_SWAP-70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000172">
        <property type="entry name" value="differentially expressed in FDCP 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057836">
        <property type="entry name" value="EF-hand_SWAP70_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057837">
        <property type="entry name" value="PH_SWAP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14383:SF2">
        <property type="entry name" value="DIFFERENTIALLY EXPRESSED IN FDCP 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14383">
        <property type="entry name" value="SWAP-70 RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25530">
        <property type="entry name" value="EF-hand_SWAP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="213"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908063.1"/>
        </source>
    </evidence>
    <sequence length="629" mass="73827" checksum="5E640C019AC5D164" modified="2020-10-07" version="1">MELRTELLKSIWYAFTALDAEKSGKVSKSQLKVLSHNLYTVLCIPHDPVALEEHFKDDDDGPVSSQGYMPYLNKYILDKVAEGAFVKESFDELCWTLTAKKNYNPDRNGNSVVSHGDAFKLWCLFNFLSEDKYPLVMVPDEVEYLLKKICTAMSIELNSGELDDYFSQETQQQNGLIVWQFLDLVNSGRLLRGIEREPISMAIEEVYQEIIGDVLKQGYLWKKGHLRRNWCERWFTLKPSDLAYYVSEERKEKKGSICLDRNSCVEILPDKDGKRCMFCVKTSSRTYEMSASDTKQRQEWTLAIQMAIRLQAEGKKSLHKDLKQKRREQREQRERKKAAKEEEMQRLKQLQEEKERKLQELELLKEAQKQAELLLQEEEQRRQQQHEEMQKMLEIQLQEAEQARASMQAEMVLKEAEAERQRKRIAELEDMQERLQDALQQEIKARQDEGAVRYEQARLLAEEEEKLKQLMKLKEEQEDYIIKAQHEKLALKQEMESKSKFLEETKLQLEEVRFNRERMDQDVMIAQQKLQQASTNVKHWNIQMNRLMHPIAPGEKRTMTSGGFPGFNPAILSKRESSVKLKQNMEKKKSDPEKNESKENINGETEKQMLMPEKDDSGSETPCLASEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAS5</accession>
    <name>A0A6I9XAS5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">High mobility group protein HMGI-C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HMGA2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMGA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908313.1">
        <property type="nucleotide sequence ID" value="XM_014052838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAS5"/>
    <dbReference type="GeneID" id="106538375"/>
    <dbReference type="KEGG" id="tsr:106538375"/>
    <dbReference type="CTD" id="8091"/>
    <dbReference type="OrthoDB" id="9946651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000116">
        <property type="entry name" value="HMGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000637">
        <property type="entry name" value="HMGI/Y_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23341:SF4">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN HMGI-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23341">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEINS HMG-A AND C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00930">
        <property type="entry name" value="HIGHMOBLTYIY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00354">
        <property type="entry name" value="HMGI_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013908313.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="12090" checksum="B08C64DA9772204F" modified="2020-10-07" version="1">MSARGDGAGQPSTSSAAAKEQPAAAEPPKRGRGRPRKQPQETTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWPQQVVQKKPTKDQPEETSSQESAEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAY4</accession>
    <name>A0A6I9XAY4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Potassium channel subfamily K member 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106541748</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912754.1">
        <property type="nucleotide sequence ID" value="XM_014057279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAY4"/>
    <dbReference type="GeneID" id="106541748"/>
    <dbReference type="KEGG" id="tsr:106541748"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000070">
        <property type="entry name" value="Potassium channel, subfamily K, member 12 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005410">
        <property type="entry name" value="2pore_dom_K_chnl_THIK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF11">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01588">
        <property type="entry name" value="THIKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="95"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="208"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013912754.1"/>
        </source>
    </evidence>
    <sequence length="412" mass="45813" checksum="49F4776A4B4A3B20" modified="2020-10-07" version="1">MMSIRRLKGCCCMSHFNEDNGRFVLLAVLIGAYLTVGATIFSAIESPSEAEAQHRWNGTLHNFSQIFNISVPELKAFLRSYEAAMAAGIRVDALRPRWDFTGAFYFVGTVVSTIGFGMSTPATVAGKAFLIVYGLFGCAGTILFFNLFLERIISLLALVMKACRERQLRRSGLLPPTCRRGSALSDADSLIGWKPSVYHVMLILGIFAITIACCASAMYTAVEGWNYIDSLYYCFVTFSTIGFGDLVSSQNAVYQNQGLYRFGNFTFILMGVCCIYSLFNVISIVIKQVLNWMLRKFRCRCCPDCNKPNTRLGRRNAITPGSRFRRHNFSMETDGQYDSDTEGRRLSGEMISMRDLTASNKVSLALLQKQLSETANGYPRNVCISTRQNDFSGGVGALAIMNNRLAETSDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XB34</accession>
    <name>A0A6I9XB34_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Transmembrane and ubiquitin-like domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">TMUB2</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911041.1">
        <property type="nucleotide sequence ID" value="XM_014055566.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911042.1">
        <property type="nucleotide sequence ID" value="XM_014055567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XB34"/>
    <dbReference type="GeneID" id="106540463"/>
    <dbReference type="KEGG" id="tsr:106540463"/>
    <dbReference type="CTD" id="79089"/>
    <dbReference type="OrthoDB" id="161999at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17132">
        <property type="entry name" value="Ubl_TMUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040352">
        <property type="entry name" value="TMUB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557">
        <property type="entry name" value="PROTEIN C7ORF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557:SF4">
        <property type="entry name" value="TRANSMEMBRANE AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="155"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="71"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013911042.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="32251" checksum="D59FCFE98A2ADBA4" modified="2020-10-07" version="1">MEPPEITIIEGVGDEVTVVAGIVVLIMALVLAWLSTYVADGSNHLLGTVVANGDSSVIHLNPIENYVGNSVSEQSEPQNATENTEEKADEGSASGTSPTVEQVDNSSGADTLDCLLDIQGLPQRTLSIDGVSQESQGVPQTVANMEESEPNTGFIKVRLKFLNDTEEVAIVKPEDTIGILKSKYFPGQESQMKFIYQGQLLQDQARSLQSLNILDNCVIHCHLSQTVSAIPDTVVAPSEAGGITLSMSDLMIPIFMLMLAVIWYFRINYRQLFTAPATISLVGVTVFFSFLVFGMYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC71</accession>
    <name>A0A6I9XC71_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">45 kDa calcium-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Stromal cell-derived factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SDF4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CREC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911861.1">
        <property type="nucleotide sequence ID" value="XM_014056386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC71"/>
    <dbReference type="GeneID" id="106541058"/>
    <dbReference type="KEGG" id="tsr:106541058"/>
    <dbReference type="CTD" id="51150"/>
    <dbReference type="OrthoDB" id="9978834at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16225">
        <property type="entry name" value="EFh_CREC_cab45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000207">
        <property type="entry name" value="Putative 45 kDa calcium-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027240">
        <property type="entry name" value="CAB45_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF98">
        <property type="entry name" value="45 KDA CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="182"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="227"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013911861.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="36483" checksum="EC973EE462B163B1" modified="2020-10-07" version="1">MVSKRVFICSLGSVYLSLLFVFLLMDVYARPANSSVLKEKMADNKDDNEILPPDHLNGVKMELDGHLNKEFHQEVFLGKEMEEFEEDSEPRRNRKKLVVIFSKCHVSWDEYKIKFLASKGMNEKEVADKIKNNEELKIDEETQEVLDNLKDRWYQADNPPADLLLNEEEFLSFLHPEHSRGMLKFMVKEIVRDLDQDGDKKLTLSEFISLPVGTVENQQAQDVDDDWVKDRKKEFEDVIDADRNGIVTMEELEEYMDPMNEYNALNEAKQMIAVADENQNHHLELEEILKYSEYFTGSKLMDYARNVHEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCA6</accession>
    <name>A0A6I9XCA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">SLC6A17</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911487.1">
        <property type="nucleotide sequence ID" value="XM_014056012.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911488.1">
        <property type="nucleotide sequence ID" value="XM_014056013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCA6"/>
    <dbReference type="GeneID" id="106540765"/>
    <dbReference type="KEGG" id="tsr:106540765"/>
    <dbReference type="CTD" id="388662"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032328">
        <property type="term" value="P:alanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015816">
        <property type="term" value="P:glycine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015820">
        <property type="term" value="P:L-leucine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002438">
        <property type="entry name" value="Neutral_aa_SLC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
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    <sequence length="725" mass="81237" checksum="86C71C1890A031D6" modified="2020-10-07" version="1">MPKSSKVTQREQSNEPVTESVADLLALEEPVDYRHSVLNVASENWNKQQVGEEAEDDRPAWNNKLQYILAQIGYSVGLGNVWRFPYLCQKNGGGAYLVPYFILLILIGLPLFFLELAVGQRIRRGSIGVWNYVCPRLGGIGFASLLVSLFVGLYYNVIIGWSIFYFFKSFQYPLPWSECPIFKNGSVAIIEPECDKSSATTFFWYRDALDISSSISESGGLNWRMTLCLLTAWFIVGLAMIKGIQSSGKVMYFSSLFPYVVLICFLIRGLLLRGAVDGILHMFTPKLDKMLDLQVWREAATQVFFALGLGFGGVIAFSSYNKQDNNCHFDAALVSFINFFTSILATLVVFAVLGFKANVMNEKCVVENAEKILGYLNTDILSHALIPPQVNFSHLTAKDYTEMYAVIKTVKKDQFENLGLDPCLLEDELNKSVQGTGLAFIAFTEAMTHFPASPFWSVMFFLMLINLGLGSMIGTMAGITTPIIDMFKVRKEALTVGCCIFAFLVGLIFVQRSGNYFVTMFDDYSATLPLTLVVILENITVAWIYGTKKFMQELTEMLGFRPSSYYFYTWKYISPICMAGLMTASIIQLAVNPPGYSAWIREEATEKFLYYPKWAMGILVSLIILATLPLPVIFILRQFHLLSDGSNALSVTYKKGRMMKDISNLEDNDETRFILSKVPSETPSPMPAHRSYLGPGSTSPMEMSNVANGRYSSGYLMDTTPESEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCS6</accession>
    <name>A0A6I9XCS6_9SAUR</name>
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        <recommendedName>
            <fullName evidence="10">Nipped-B protein</fullName>
        </recommendedName>
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        <name evidence="14" type="primary">NIPBL</name>
    </gene>
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        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014057924.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
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    <dbReference type="GO" id="GO:0140588">
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    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:establishment of protein localization to chromatin"/>
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    </dbReference>
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        <property type="term" value="P:regulation of gene expression"/>
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    <dbReference type="InterPro" id="IPR033031">
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    <dbReference type="PANTHER" id="PTHR21704">
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    <dbReference type="Pfam" id="PF12765">
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    </dbReference>
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    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="12">
        <location>
            <begin position="2233"/>
            <end position="2413"/>
        </location>
    </feature>
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            <begin position="602"/>
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        </location>
    </feature>
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        <location>
            <begin position="976"/>
            <end position="1004"/>
        </location>
    </feature>
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        <location>
            <begin position="1016"/>
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        </location>
    </feature>
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        <location>
            <begin position="1647"/>
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        </location>
    </feature>
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        <location>
            <begin position="2434"/>
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    </feature>
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        <location>
            <begin position="2616"/>
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        </location>
    </feature>
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        <location>
            <begin position="130"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="201"/>
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        </location>
    </feature>
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        <location>
            <begin position="262"/>
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        </location>
    </feature>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913399.1"/>
        </source>
    </evidence>
    <sequence length="2768" mass="313020" checksum="1B15AEF402BF58BD" modified="2020-10-07" version="1">MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNGRIAEEVNCLLACRDENLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARNPNVFREKSMQNRYGVQSGMMMPQYKLSQNSMHGSPASSNYQQTTISHSPSSRFVPPQSSSGNRFMAPQNSPVPSPYAPQSPAGYLPYSHPPSYTPHPQMQQASVSSPIVSGGMRNIHENKITSQLGSSTNHHPDNTRHGSSEDYLHMVHRISSEDGDSSIRNTTSFSLRSPQSVCSPAGSDGTPKGSRPPLILPSQPPPYSSPRDVPPDILLDSPERKQKKQKKMKLVKDEKDQTEKAAMYDIISSPSKDSTKLTLRLSRIRSSDMDQQDDMLSGLENSNASEGDISFNVQYPGQTSKTPVTPQDINRPLNAAQCLPQHEQTAFLQAQQVPVLQPNTSVSAKQPQNPLVQTHPQVSQHGTITPYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSFPQEAGGATGGNKPASQDTNSAGNGSRPALMVSIDLQQAGRADSQAVLTQDLDIIKKPEEIKQCNDGSLFIAQDDVIGVLKLKTENFPETLRKKSDSEGIKMEVQHNENKQTGIQQNEGKRIEIKHCESKQAEVKHESKHENRQVDVKQNEAKQNNGKQEARQRPETPKQKNEVRPDTPKQKNEGRPETPKQKNEGRPETPKHRHENRRDSNKSSLEKKLDVSKHKMDIKSDPSRVKSEKSDSLKQRTDGRSISDSVRRDHDGLKQKTEDRGESERYRGDPSKLRRPEYLRSSSKSEHDSKHIIRTDNTKAERLDRKHRHESGESRDKLSSGDQRSRPDSPRIKQESKLRTDRPSFKSQNSKDDRRTDGNKSKMDSSKAHGDSKTEFPSYLLGGRSGALKHFIIPKIKRDKDGNVMHEVRKSEFKGEPKNKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKFIKDREDKSRSSCFRSNKNKSSKSSKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNESEEAFETSRKRHRKDRNDDKAWEYVEHDRRSGDHKRSAHYHEGRRSSGSSRYRGRSPEDSDVDDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFSASVENILDNLEDMDFTAFGDDDEMPQELLLGKHQLSELGSESAKIKAMGIMDRLSTEKTVKVLNILEKNIQDGAKLSTLLNHNNDTEDEERLWRDLIMERVTKSADACLTAINIMTSPHMPKAVYIEDVIERVIQYTKFHLQNTIYPQYDPVYRLDPHGGGVLSSKAKRAKCSTHKQRVIVMLYNKICDIVSSLAELLEIQLLTDTTILQISSTGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDTDGEPMYIQMVTALVLQLIQCVVHLPSGEKDSNSEEESNKKIDQDVLITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIARILKQVSGGDDEIQQLQKALLDYLEENTETDASLLFSRKFYIAQWFRDTTMETEKAMKSQKDEDSSEGTHHSKEVETTGQIMHRAEGRKKFLRKIIKTAPSQFSTLKMNSDTVDYEDACLVVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDASYKPVKKACTQLVDNLVEHILKYEESLADSDNKGLNSSRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSNQNDFMVICNVAKILELVVPLMEHPSETFLATMEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGALLKLKSQHQEDPNSTILTANKPALLRSLFTVGALCRHFDFDQEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPGLMFEQEVKTLYNNILSDKNCSVNLKIQVLKNLQTYLQEEDTRMQQADREWKKVAKQEDLKEMGDISSGMSSSIMQLYLKQVLEAFFHAQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPSMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCRKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVNMLLYIADNLGCFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKYKKEKKPSSDEESESESNSESDSESEEEIQKPRKSSRKRANSDSDTDEENDINAVMKCLPDNSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDTKIQKYSPSESAKVYDKAINRKAGIHFHPKQTLDFLRSDMANSRLTEDVKRNIVKQYLDFKLLMEHLDPDEEEEDGEVSASTNARNKAITSLLGGGSPRNNAAETEEDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSNGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCX3</accession>
    <name>A0A6I9XCX3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PDE7A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013911697.1">
        <property type="nucleotide sequence ID" value="XM_014056222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCX3"/>
    <dbReference type="GeneID" id="106540918"/>
    <dbReference type="KEGG" id="tsr:106540918"/>
    <dbReference type="CTD" id="5150"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000004">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="136"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="452"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="212"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911697.1"/>
        </source>
    </evidence>
    <sequence length="484" mass="55728" checksum="4F1C0A6ED9D3C73B" modified="2020-10-07" version="1">MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPNPRQLSQRRGAISYDSSDQTALYIRMLGDVRVRSRAGFERERRGSHPYIDFRIFHSNSEFEVSVSARNIRRLLSFQRYLRSSRFFRGITLPNSSNILDDDYNGQAKCMLEKVGNWNFDIFLFDRLTNGNSLVTLTFHLFNLHGLIEYFQLDTMKLRRFLVMVQEDYHSHNPYHNAVHAADVTQAMHCYLNEPKLFQSLTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNTSVLENHHWRSAVGLLRESGLFAHMSLESRQLMESQIGALILATHISRQNEYLSLFRTHLDKEDLCLLEEDHRHLVLQMALKCADICNPCRTWELSKQWSEKVTEEFFNQGDIEKKYCLGVSPLCDRQTETVANIQIGFMTYLVEPLFAEWARFSNTKLSQTMLGHMGLNKASWKGIQREQSSSGDDTDAACEETDSDVVPQEHQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XE51</accession>
    <name>A0A6I9XE51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Midasin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106541520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912476.1">
        <property type="nucleotide sequence ID" value="XM_014057001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XE51"/>
    <dbReference type="GeneID" id="106541520"/>
    <dbReference type="KEGG" id="tsr:106541520"/>
    <dbReference type="OrthoDB" id="422220at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01460">
        <property type="entry name" value="vWA_midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000028">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="1064"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="362"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="437"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="474"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="506"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="601"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="630"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="670"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="696"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="709"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="739"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="771"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="827"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="863"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="877"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013912476.1"/>
        </source>
    </evidence>
    <sequence length="1276" mass="144524" checksum="84E4C11227729356" modified="2020-10-07" version="1">MQCVVLLEQLSWFIECCPEDQTLNTSRTEIKSEGVELNSSTILKSLPELDPSELQYLTPVSPEQLPAACRMRKQDTIYQQLASKVKEMLVEVKAVKSDVDRIKQVSSETLFHSWKSFEVCSSGVDYLLKVSTQLQDIELLFTPPDSQVNQIGSQMALIRSLIYIQGEINSSVDDFAIWKTQLFSSISHCEDNETSNDGFVEHFSEEIELAIHVVLSSVQCLVERRKDNEEIFPKEDGEESSVDGLQTGHLTKLLDEEICSDVNSLHIQKIISVISELLVELKTYGEDYTAYKCKLFNQACHLLVRLMPILCKYSNIVLFYLTVSLATHRSTAKLLFVLTNIFTEIAQKGFCLPKEIIEDGGQGGTEFHDYEDGGIGEGEGKKDVSDKIENEDQVEDTYQKGQEKEKEDPDSKPDIEGEDNAIEMSEDFEGKTYDGDQEKEEEDDKSDEDEDLDKQMGNLGDGDADKLDERLWGDDDEEEEEEEEEEEEEDGKTETGPGMDEGDSELVAKDDNIGVDNQNEKKSPNDEEKEKQADDSETKEKIHEQIDEGEYDENEVDPYHGRQETQPEPEALELDHELNLGNEDMDEEDKEEEEGENPFEIEEKAIDVDEKEAEDKNTGDMDEDGQDSEQVEKDPSEDKIEQDKNEEDKNEEGTENSDANTGEEEDSTVEEQKAEEKEQNISTADQGLCPQKIDEAGNDTENEEQLPEALERMEHEAYGETGEENLQSDTAVEMVGTASEKDQAKEEHGSGTSDANQPEGHESELMAHLASQKQSRKNTQSYKRKPGQADNERAPGDQSQPVHKRLRTIESSQEADERPVQPGQRQVEADTFEHIKQGSESYDAQTYDVASEEQQKPILPVNEDNEETISDDLAMETEDHAAEELKTSEVEKLNPEAKMSGTSKSGPEQIEADSQVPNSEDAEERESNSLLPEVKQDRNKHSTIHTAHQFLKDPNAQHIFKNPEELRQELEKQLEAWKIRNPLEGDAVAEEMWQRYLLLTEPLSQQLCEQLRLLLEPTQAAKLKGDYRTGKRLNMRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLALDDSASMVDNHSKQLAYESLAVIGNALTLLEVGQIAVCSFGETVQLLHPFHEQFNDRSGARLLQLCKFEQKKTKIAQFLESSINMFAVAQHLSSNINPETAQLLLIVSDGRGLFLEGKERVKAAVQSVQNANIFVIFVILDNPNSRDSVLDIKVPVFTRPGELPEIRSYMEEFPFPFYIILRDVNALPETLSDALRQWFELVTASDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XE86</accession>
    <name>A0A6I9XE86_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rho guanine nucleotide exchange factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106542680</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913954.1">
        <property type="nucleotide sequence ID" value="XM_014058479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XE86"/>
    <dbReference type="GeneID" id="106542680"/>
    <dbReference type="KEGG" id="tsr:106542680"/>
    <dbReference type="OrthoDB" id="2272012at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000006">
        <property type="entry name" value="Rho guanine nucleotide exchange factor (GEF) 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015212">
        <property type="entry name" value="RGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45872:SF4">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45872">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09128">
        <property type="entry name" value="RGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="491"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="273"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913954.1"/>
        </source>
    </evidence>
    <sequence length="733" mass="83373" checksum="40892084F698F471" modified="2020-10-07" version="1">MELEEGYNGSSALGAQGGVAMSIIGAEDEDFENDIEPFQHPDAQTSLFQNLEILMKHPAYLMVFIQHVVLQFDSSPVLCYLHVDLILNRLGPKEAKKQFVDFCHVFLDKTGLLRVPIPTVVQFELDRNRPDLIPEDQLKKYLQDIQAFQEVEISQQLEDFREKRKMGMTPGEKELFELEGYRTKDKGIREAKEKQLAEQLLVKLEEMHLTISTDEEKSSAIYTAIVTYMKHLGVKAKTADNKKSKANFFRKKISGTKKPEEAIKSRKGFSILDPARWNRGDSHSAEKTPALDKKAKLSEGTSGRSKGNPFVQDPSSVSVTVHPPPGESNDGEQGLDSPTLGADSGDSPVTEQALAGEQLPLEESTENERRRKRLTGKLGRSESLRVYERKRSQRGSAKGKQPRSRSDVDIEAAARANELEGKPTLEQARQKQGSRNLEPGGAGGGEPPPSFLLPQSEEFEPRVSELELDPPNWRELVPPDTLLRLKKSEVKRQEVINELFITEHAHLRMLRVLLEVFYQPLLTEGFFTESELVNIFPSLEDLIDEHKVFLENMKRLREENNLIVSEIGSTLLARFDGSEGNWFQKISSRFCSRQSFALEQLKAKQKKEARFNQFIQEAESKPRCRRLQLKDIIPIEMQRLTKYPLLLHSIAKCTEEAEERQKVEKAAECCRQILNHVNQEVRVMENLLKLKDYQRRLDLSNLKQSTDPLLSEFRVSPNAVGTEEAMTVNELPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFC5</accession>
    <name>A0A6I9XFC5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein SLX4IP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SLX4IP</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914369.1">
        <property type="nucleotide sequence ID" value="XM_014058894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFC5"/>
    <dbReference type="GeneID" id="106543006"/>
    <dbReference type="KEGG" id="tsr:106543006"/>
    <dbReference type="CTD" id="128710"/>
    <dbReference type="OrthoDB" id="9933290at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031479">
        <property type="entry name" value="SLX4IP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28557">
        <property type="entry name" value="PROTEIN SLX4IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28557:SF1">
        <property type="entry name" value="PROTEIN SLX4IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15744">
        <property type="entry name" value="UPF0492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013914369.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13823" checksum="9E80B951B31387D1" modified="2020-10-07" version="1">MTLDKLVIKCGNFAVLVDFHIASQDRMWFSDHQREEVCLLLKDAVESRVNQYLKARKLHGRGKQTEHGESNSLSLRADEFHISAYFVKRWANLRCIGQRHGGKLPMSPFGVLWRNKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFD0</accession>
    <name>A0A6I9XFD0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pecanex-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">PCNXL4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the pecanex family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912582.1">
        <property type="nucleotide sequence ID" value="XM_014057107.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912583.1">
        <property type="nucleotide sequence ID" value="XM_014057108.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912584.1">
        <property type="nucleotide sequence ID" value="XM_014057109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFD0"/>
    <dbReference type="GeneID" id="106541605"/>
    <dbReference type="KEGG" id="tsr:106541605"/>
    <dbReference type="CTD" id="64430"/>
    <dbReference type="OrthoDB" id="5979286at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039797">
        <property type="entry name" value="Pecanex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007735">
        <property type="entry name" value="Pecanex_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12372">
        <property type="entry name" value="PECANEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12372:SF6">
        <property type="entry name" value="PECANEX-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05041">
        <property type="entry name" value="Pecanex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="575"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Pecanex C-terminal" evidence="8">
        <location>
            <begin position="1027"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="795"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="815"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="832"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013912584.1"/>
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    </evidence>
    <sequence length="1189" mass="134763" checksum="DC5D6B9BB312C238" modified="2020-10-07" version="1">MGPDVPLLNDHKQEFLLKRFPQSILGGPQFKLGYCAPSYIYLNQILLFLIPWILGGIGTLMYQLNVLQDYGTAALSGGLMLVAAIIVQGINLYARQKTAPVERMHVHNILTEEDEWEFSSCIGLETIKFIIPGKKYIINVFFHSLVAGVLCGLGTWYLLPSRIASLYGDNIGTTVVIFVFGWLTVCIGEYSLIVNTASETATYQALDTYEIVPLMRPFYIFVFIAVDLAHRFVADAAALKLVNQVMHIVFLSLPLLWTLGILPPLDALFLWGMEQVLELGLGGSPMSNNVRLLAMFIISLGTAVASYFIPNTLGVVLFMTGCGFILSLNLNEIGLTLKYTMISCLASSKFKSTSSCRTQFVWKELMFYFIVLTFALLEAGLLHFFLGFQTFSKTSLQGVISYILIVLLLAQWILREVQTVYLFGLFRNPFYPKDLMTTNVSGRKEKNLKKIGMLRKILLTLVSPFAMIAFLSIDNSLHQLSSIYISIGFSRSFRMVWQDTEHALLDMVVVSAVQLLVFTTDLWWNKSIDTGIRLLLVGIIRTRLFQFVSKLQFAITVLLTSWTEKKQRRKSTATLITLNIAFFPIVLAIIVLSSLLSSPLLSLFSLPIFLIGFPRPVRSWPGPVGMMTCVGPDAVYYQQMIPSLRTALQSAFSTGRLGFCSPGSHYLCRFQDRLIWILVLERGYTYCCLNIKGLELQETSCHTAEAHRVDDIFAMAFEHQEHNKMFSLNPHFGNILTPCAALPVKLYSDARNTLSGIIDSPENRKQFKEDFIKVLLWMLIQYCYKKSKTPRPLEKMCKPQQEYSSAGPSEVLNKTIERSDSWKDEDGLSMESTEDWTDDSNLFDDEPSKKTLKTNVTVPQDGSPRPFYSLPGSVEIHTTDDEVLEVASVNKLYKTVVFGLPVVDAGKHWGNMDSSPIKFSNFYSELLSIPEEWRTAPLSAAKFHQMKQKFPVEWYHFLLGQLNLFHLKENPSDLLKDLFKDTALKDLYAQSALSCYIAMFGLDDTIVSPGHIFRAYNGSLPWSVCLEWLIGKQELYQLTLKTFRYTVKLMVDKASLGPVEDFQELLNYLKEYENDWYIGLVSEKEWPQAVLQETPYLFSLGHDPNMGVYTGRILTLQEFRVQVGKLNEEAVRGQWGNLSWELLYATNDDEERYSIQAHPILLRNLTIQAADPPLGYPVYSSEPLHLTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHN2</accession>
    <name>A0A6I9XHN2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">cAMP-dependent protein kinase type II-beta regulatory subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106542253</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014057917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHN2"/>
    <dbReference type="GeneID" id="106542253"/>
    <dbReference type="KEGG" id="tsr:106542253"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000027">
        <property type="entry name" value="Protein kinase cAMP-dependent type II regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF156">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE II-BETA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0114">cAMP</keyword>
    <keyword evidence="3 7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="114"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013913392.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="28541" checksum="814F4E9535604BAF" modified="2020-10-07" version="1">MSQVLDAMFEKLVEGGEHVIDQGDDGDNFYVIDEGTYNIVVNCDGVGRCVGTYDNRGSFGELALMYNTPRAATIIATSPGTIWGLDRVTFRRIIVKNNAKKRRMYENFIESLPFLKSLELSERLKVVDVIGTKIYKDGEQIIVQGDTADSFYIVESGEVKITMKRKGKQDIDGNEAVEIARYTRGQYFGELALVTNKPRAASAFALGTVKCLIMDVQAFERLLGPCMEIMKRNIANYEEQLVALFGTNMDISEPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ17</accession>
    <name>A0A6I9XJ17_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 42A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CCDC42</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910918.1">
        <property type="nucleotide sequence ID" value="XM_014055443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJ17"/>
    <dbReference type="GeneID" id="106540360"/>
    <dbReference type="KEGG" id="tsr:106540360"/>
    <dbReference type="CTD" id="146849"/>
    <dbReference type="OrthoDB" id="2134857at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF8">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="3">
        <location>
            <begin position="42"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="103"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013910918.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="23158" checksum="65A5D3BA3DB5782C" modified="2020-10-07" version="1">MTTMDEEDLSEYFRMQYGQKLLKLLMKFPVTEEQSPSPSIRLLEKKKEAKLVHQAMEAQKEAFKTRMESLNNRWEELGTKEVQLKAYIKKFEQFIQENDQKRIRALKKANKERELKKQRVKELAKVKLEIAALKQQHQKLYNKLQQYSIFNKYLEKVVQVSEIQQFIQDLSDIWGEVKKKDQNRSQIGFPKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ24</accession>
    <name>A0A6I9XJ24_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger CCHC domain-containing protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ZCCHC8</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915764.1">
        <property type="nucleotide sequence ID" value="XM_014060289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJ24"/>
    <dbReference type="GeneID" id="106544126"/>
    <dbReference type="KEGG" id="tsr:106544126"/>
    <dbReference type="CTD" id="55596"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052115">
        <property type="entry name" value="NEXT_complex_subunit_ZCCHC8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13316:SF0">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13316">
        <property type="entry name" value="ZINC FINGER, CCHC DOMAIN CONTAINING 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915764.1"/>
        </source>
    </evidence>
    <sequence length="589" mass="65007" checksum="F1058680C12ED51A" modified="2020-10-07" version="1">MAAEVDFGDRELFQQLEADEEAPGLEPDPAEEASELRERLRESEETIRQLREENQELRRKLNNLARPSGLTVDDCRLDGPLLQILFMNNDVSKQYHQEVEDFVSSLVQKYEEQRRSELDKACFNVKPQPSSFVLEEDHKITSSNVSKKIKEAFSVVGSVLYFTNFCLDKLGQPILNENPQLTDGWEIPKYQQVFTQILSLEGQEIQVKSKSMSSSASSEGEEESHQQNPCVSYDLSKLINYPGFNMSTPSGVTDEWRMFGSVPLQPSQQKEIFAHYLSTYHSANLKSKPKRPPPRQSSHHSKRQKGSSAEVSPADMEIDSDLEMPPSFPDYDGFHFQPPLPPGSPLTATPPPLPQGTPPATPPSLTPPQPLSYVPAALPWTPSAPRPEDSAMDEDTLTLEELEEQQRLIWAALEQAESGNSDSDLPAGTPMASNSGTSSPCRTELSIALEQAGPQQLKEVSKAEPTDKPEQIPQTPEEPPEEPGPQPAIELVDLTAEREAIPTDLGGQTCSSSDPGGLLNHSDAPEIPSASQPPPSTPPGLVPDMSKFAAGITPFEYDNLSESTGTYLRIRSALKKYPRSQLKNKKLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLM9</accession>
    <name>A0A6I9XLM9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular tyrosine-protein kinase PKDCC-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106543350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914827.1">
        <property type="nucleotide sequence ID" value="XM_014059352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLM9"/>
    <dbReference type="GeneID" id="106543350"/>
    <dbReference type="KEGG" id="tsr:106543350"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042983">
        <property type="entry name" value="PKDCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448:SF2">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013914827.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="18401" checksum="24705807974C817A" modified="2020-10-07" version="1">MENTRYFFTYLLPYTAPIALQPILKDILNATGDLRYGVNETLRAFQKVLHLYKSGLYLQKKPSHLKEYFVAKGFHSMDTEDYKCWPSYSPLRCMLSVHNVEEAASICSYHPQCQHFIITSQRTWTGRPLAIFQSNLTYLIPGSTMEVYVKRSAFSRGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP06</accession>
    <name>A0A6I9XP06_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="23 24">Protocadherin Fat 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23 24" type="primary">FAT1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23 24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917724.1">
        <property type="nucleotide sequence ID" value="XM_014062249.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917725.1">
        <property type="nucleotide sequence ID" value="XM_014062250.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106545614"/>
    <dbReference type="KEGG" id="tsr:106545614"/>
    <dbReference type="CTD" id="2195"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000152">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000154">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000015">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000021">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000026">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000037">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000041">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000051">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000052">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000064">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000065">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000067">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000071">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000075">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000079">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000080">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000089">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000107">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000161">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000053">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000058">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000059">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000061">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000084">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000057">
        <property type="entry name" value="protocadherin Fat 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000035">
        <property type="entry name" value="Protocadherin Fat 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="F8A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16797:SF4">
        <property type="entry name" value="40-KDA HUNTINGTIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16797">
        <property type="entry name" value="FACTOR VIII-ASSOCIATED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915886.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="30862" checksum="B6DE381B3418279B" modified="2020-10-07" version="1">QESLPYAAWCQLAVARCAQSLGHPAGEAAALAEAARLWLRHERELRLRQGPGLGLSEYLPAAHGCVAFAARLHVDLGQPALAAGLWLELGNELRSAGEPSQAVPAFLRAADLLAAGHLPLEALHGLRDVASCLLLSRDYDGALAMFTQAQQLAQGGGGPSTNAPASSSSSSSSSSCGGAPSLPGAFLDHVLHCEVTRLLLLLLLQPPPSKLLPEHAQILEKYSWEALDASSGYVPPELFLLLQSAVMACQEKDLESLKVLQKELWPLLTAEQNNLLHLVVQEMITPSGQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQV5</accession>
    <name>A0A6I9XQV5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubby-related protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Tubby superfamily protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Tubby-like protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TULP4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913063.1">
        <property type="nucleotide sequence ID" value="XM_014057588.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541990"/>
    <dbReference type="KEGG" id="tsr:106541990"/>
    <dbReference type="CTD" id="56995"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000262">
        <property type="entry name" value="Tubby like protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.90.10:FF:000002">
        <property type="entry name" value="Tubby like protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056159">
        <property type="entry name" value="Beta-prop_IFT121_TULP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF2">
        <property type="entry name" value="TUBBY-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24797">
        <property type="entry name" value="Beta-prop_WDR35_TULP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="16">
        <location>
            <begin position="373"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="828"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1421"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="845"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913063.1"/>
        </source>
    </evidence>
    <sequence length="1585" mass="174609" checksum="EB804FB59048F0F4" modified="2020-10-07" version="1">MYAAVEHGPVLFSDSNILCLSWKGRVPKSEKEKPVCRRRYYEEGWLATGNGRGVVGVTFTSSHCRKDRNTPQRINFNLRGHNSEVVLVRWNEPFQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHVLLHESDGILSMSWNYPSFLVEDSSESDTDSDDYSPPQDGPAVYPVLVQNTKQLLTVSFTSGDISLMNNYDDLSPTTIRSGLKDVVAQWCTQGDLLAVAGMEKLSQLAELNAASFLKSALVKFYNIHGEHIYTLETPVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQTIASSLRDDKDISKLTLPPRLCSYLTTAFTPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKADEVYGNSLISTMIDSCNCSDSSDIELSDDWAGKKSPKITRASKSPKLPRINIEARKSPKLSRAAQEISRSPRLPIRKPSIGSPSLTRREFPLEDIIQHSYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEVRKISTDYINLPVFNPNVFSEDEDDLPVTGASGVPENNPPCTVNIPIAPIHSSAQAMSPTQSIGLVQSLLANQNVQLDVLANQTTAVATADHVADNAVHYPIPSRYSNPGQVIFGGVEMGRILQVPPQLPLPPQNAIQMAIVDHRDGDRDHEHLQKAKALRPIPQVAEGDTAVFSTAQEIQLNKINPPPPYPGTIPAAPTTAPPPPPPPPVPPQPPVDLCLKKGDFSLYSAAHYQTPLGYERITTFDSSGNVEEVCRPRTRMVCAQNTYTLPGPGSSATLRITSSEKKIQQPCTSATLNRLTVPRYSIPTGDPPPYPEIAAQLAQGRSITQRLDNSIIHATLRRNSREAALKMAQLVDSQRATLQFPLKLKSNVVGAQYQQRVPTALYTCSQCSSNTNNGISNTNNGNGNISNINASSGTSGLSTMRPDVTTGSSSQHNSVIAHSASTSPLASQSSYNLLNPLDNSRERTDYVNSAFMEDEALSQHCPVEKSARHIPVAMAEPNIAVKRPPPYQWEPMVSEEIWVPQERTAHSTLPNPGKSNPLVIGQAQHLDISRATFVSPKSPTSPTATFQMSYGVGAPYLGNYSIPPPPLPPLQGMQVPCSPKEALASSQMVQQEPSVVLQPAYPSNLSYCPLPPMYPGNSTCSSLQLPPMALHPWNSYSTCPPSVQNTQNILPPKALLVVDKSIMSPPPVEPTGHVGSDVMAETADNFQEVLSLTESPISQRTDKFGKKNRKRLDSRADEGNMQAITEGKVKKEARTTLTDFNSLIASPRLGREKKKLKSQKDQLKSKKMNKMNEFQDSSESEPELFISGDELMNQSQGSKKGWKTKRNLRTASELDEFKCRKANEKEDGRLGNQGFVYVMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRC7</accession>
    <name>A0A6I9XRC7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="17">ATP-PFK</shortName>
            <shortName evidence="17">Phosphofructokinase</shortName>
            <ecNumber evidence="17">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PFKL</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 18">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="17">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17 18">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homo- and heterotetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013907260.1">
        <property type="nucleotide sequence ID" value="XM_014051785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRC7"/>
    <dbReference type="GeneID" id="106537603"/>
    <dbReference type="KEGG" id="tsr:106537603"/>
    <dbReference type="CTD" id="5211"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Eukaryotic_PFK"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
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        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
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        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041914">
        <property type="entry name" value="PFK_vert-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF14">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE, LIVER TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 17" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="25"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="409"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="17">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="17">
        <location>
            <begin position="408"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <begin position="95"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="171"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <begin position="215"/>
            <end position="217"/>
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        <ligand>
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        </ligand>
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        <location>
            <position position="271"/>
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        </ligand>
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        <location>
            <position position="299"/>
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        </ligand>
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        <location>
            <begin position="305"/>
            <end position="308"/>
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        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
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        </ligand>
    </feature>
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        <location>
            <begin position="534"/>
            <end position="538"/>
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        <location>
            <position position="572"/>
        </location>
        <ligand>
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        </ligand>
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            <begin position="579"/>
            <end position="581"/>
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        </ligand>
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        <location>
            <position position="635"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
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        <location>
            <position position="661"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
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        </ligand>
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        <location>
            <begin position="667"/>
            <end position="670"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRQ6</accession>
    <name>A0A6I9XRQ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/threonine-protein kinase LMTK2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106542242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913383.1">
        <property type="nucleotide sequence ID" value="XM_014057908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRQ6"/>
    <dbReference type="GeneID" id="106542242"/>
    <dbReference type="KEGG" id="tsr:106542242"/>
    <dbReference type="OrthoDB" id="8951227at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24417">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE LMTK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24417:SF8">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE LMTK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026946025" evidence="2">
        <location>
            <begin position="28"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913383.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="17017" checksum="C5C9EF02BEC57307" modified="2020-10-07" version="1">MPGRLLPPGIGWGGLGLLCCWLGGGQTAPLPPAGPGEETAFEVSYSLVVLSAGGLLVLILLLVNCISCCKDQEINFKEFEDNFDDELDFTPPAEDTPTNQSPAEVFTLTVPSVSLPAPSQFQLPTGRSVIPIWQLPFLSCPLHHGTRWSSAGVTFKFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSC4</accession>
    <name>A0A6I9XSC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106544785</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013916646.1">
        <property type="nucleotide sequence ID" value="XM_014061171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSC4"/>
    <dbReference type="GeneID" id="106544785"/>
    <dbReference type="KEGG" id="tsr:106544785"/>
    <dbReference type="OrthoDB" id="9995210at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000019">
        <property type="entry name" value="Glutaminase liver isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF33">
        <property type="entry name" value="GLUTAMINASE LIVER ISOFORM, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013916646.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="33640" checksum="5541F717C91F9EF1" modified="2020-10-07" version="1">MAGNEYVGFSNTTFQSEKEIGDRNYAIGYYLKDKKCFPCGADMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGECVLSDEAIRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGVMCLSPPLDKAGNSTRGIGFCQELVSLFNFHNYDNLLHCTRKMDPRREGDEVRNKTVVNLLFAAHSGDISALRRFALSAMDMEQKDYDSRTALHVAAAEGHLEVVKFLIEACRVNPFVKDRWGNIALDDAIQFHHLEVVKLLKDYQESYILGSRKVKEAAEDLSKENLESMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSG1</accession>
    <name>A0A6I9XSG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">L-amino-acid oxidase</fullName>
            <ecNumber evidence="2">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">OSGIN1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013916681.1">
        <property type="nucleotide sequence ID" value="XM_014061206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSG1"/>
    <dbReference type="GeneID" id="106544816"/>
    <dbReference type="KEGG" id="tsr:106544816"/>
    <dbReference type="CTD" id="29948"/>
    <dbReference type="OrthoDB" id="412005at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029731">
        <property type="entry name" value="OSGIN1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15192:SF15">
        <property type="entry name" value="OXIDATIVE STRESS-INDUCED GROWTH INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15192">
        <property type="entry name" value="PROTEIN CBG05349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013916681.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="37299" checksum="09EF967C50ABC649" modified="2020-10-07" version="1">FYRGFRNNRATAGDIVQYYQYYVAKKGLKKNFVCGTLVTSVKRLNSDSEESYGGQDSKDRSSMWDLSQGSQENGSSLFQVDGFVTNIAGTHPFSVYAENVVLATGTYDSPTHLGIQGEHLPFIHHTLSALEEAVKNKEVGVMSDPILIVGAGLTAADAVLFAHHYNIPVIHVFRRRVNDPALIFNQLPKTMYPEYHKVHQMMREQANPYPGPYEYYVSLPEHHVIRFSEDRKCIFADKSGRQKIHPISMAFVLIGSNPNLAYLPNNGMDLAVDCEQPVSSKRNPVDVDLFTYESVHEKGLYALGPLAGDNFVRFVQGGALAIASSLLKKVNKNPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSJ7</accession>
    <name>A0A6I9XSJ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">endothelin-converting enzyme 1</fullName>
            <ecNumber evidence="4">3.4.24.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106545115</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.</text>
            <dbReference type="EC" id="3.4.24.71"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.24.71" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013917052.1">
        <property type="nucleotide sequence ID" value="XM_014061577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSJ7"/>
    <dbReference type="GeneID" id="106545115"/>
    <dbReference type="KEGG" id="tsr:106545115"/>
    <dbReference type="OrthoDB" id="6475849at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF127">
        <property type="entry name" value="EEF1AKMT4-ECE2 READTHROUGH TRANSCRIPT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="13">
        <location>
            <begin position="118"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013917052.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24664" checksum="23F3B61E93640F13" modified="2020-10-07" version="1">MSVALQELGSNMTEYKRAMFQEDEVLDATGDGSNTSPDSVEVGFRKGDGLLLSRLGSQIHLKLVFWVISLFVAVLLLSTIIAMAIQYSKDPSHTTCLTEACVIVAGKILEALDPGVKPCDDFYQYACGGWIKRNPLPDGRSKWSTFNSIWDQNQAIMKHLLENATFNSSSEAERKTQRYYLSCLREQKIEELGSQPLIDLIEKIAVSEDLGFQMLEKLWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTR2</accession>
    <name>A0A6I9XTR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">TRIM24</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013914083.1">
        <property type="nucleotide sequence ID" value="XM_014058608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTR2"/>
    <dbReference type="GeneID" id="106542786"/>
    <dbReference type="KEGG" id="tsr:106542786"/>
    <dbReference type="CTD" id="8805"/>
    <dbReference type="OrthoDB" id="1870062at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19845">
        <property type="entry name" value="Bbox1_TIF1a_C-VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19828">
        <property type="entry name" value="Bbox2_TIF1a_C-VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05502">
        <property type="entry name" value="Bromo_tif1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15624">
        <property type="entry name" value="PHD_TIF1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000123">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000024">
        <property type="entry name" value="Transcription intermediary factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000074">
        <property type="entry name" value="Tripartite motif containing 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF4">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR 1-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 19" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="13 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="24">
        <location>
            <begin position="88"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="24">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="23">
        <location>
            <begin position="751"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="22">
        <location>
            <begin position="858"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="407"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="585"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="712"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="942"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="518"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="589"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="624"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013914083.1"/>
        </source>
    </evidence>
    <sequence length="976" mass="108042" checksum="2619AFD7DC15E547" modified="2020-10-07" version="1">MLPPPAAPSPGGGGGGAPKDPPLPPPPPLLQPSSAPSSPVSAPPSPLHCNPVGVVRCPICGQECAERHIIDNIFVKDSTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTNQRPVFCPYHKKEQLKLYCETCDKLTCRDCQLQEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKNKYVKYTGEQIQNRIHEVNRNQKLVEQDVKVAIFTLMVEINKKGKALLHQLECLAKEHRAKLLQQQQEVSGLSKQLEHVMNFSKWAVSSGSSTALLYSKRLITYRLRYLLRARCDALPVSNTTIQFHCDPSFWAQNIFNLGSLVIEDSELPPHMPKSPVTEPNIQSQSGLPSNQLSKFPTQISLAQLRLQHMQQQVLAQRQQAQRRAGPVGFPEPRIAGAIHQAPPPPPAPLPPPPPPQQPPPRLVNFQNHNFKSIGPVPLAQPVRFPQNQSIPHASVKPSPLQMAILAQQAIKQWQIHSGQVLATSSSASSTTSTPSSPTINSAAGNDGKTFAPSMIDLNSTMSGSCTLPSLPDIDCSGSVPLESIGKREAFAEHTQTKQYSRKTVQSPNSSVPFPGLTGLVTVSGVHPPMRSPSASSVGSRGSSTSSGKPSGADSTQRVPVVMLEPIRIKQESGAPSENFDFPVVTVKQETDEESRNQNSRFPRTILPTVLLNSNLSSEASALRTEAPDSTGDQPSNGKATWIGSSHGGDGRKDDDPNEDWCAVCQNGGELLCCDKCPKVFHLSCHVPTLRNFPSGEWICTFCRDLFKPEVEYDCGVSSHVSEEKRKVEGGPGLAPIDQRKCERLLLYLYCHEMSLAFQDPVPPTVPDYYEIIKKPMDLSTIKNRLQAQHTIYSKPEDVIADFRLIFRNCAEFNEPDSEVADAGMKLSSYFEELLKNLFSDRKFSVQPYCHSDKNSPSPTDDSDDEFVQPRKKRLKWEEHHFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV53</accession>
    <name>A0A6I9XV53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc transporter ZIP10-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106547385</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920004.1">
        <property type="nucleotide sequence ID" value="XM_014064529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV53"/>
    <dbReference type="GeneID" id="106547385"/>
    <dbReference type="KEGG" id="tsr:106547385"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF14">
        <property type="entry name" value="ZINC TRANSPORTER ZIP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013920004.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="14477" checksum="656E6CAD75C7F9B4" modified="2020-10-07" version="1">MLIIEPRGCVNVYIHSGDFAVLLKAGMTVKQAIVYNLLSAMMAYVGMLIGTAVGQYANNITLWIFAVTAGMFLYVALVDMLPEMLHGDGDNEEHGYCPVGQFILQNLGLVLGFAIMLVIALYEDKIVLDIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV61</accession>
    <name>A0A6I9XV61_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Centrosomal protein kizuna</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Polo-like kinase 1 substrate 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106543149</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the kizuna family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914543.1">
        <property type="nucleotide sequence ID" value="XM_014059068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV61"/>
    <dbReference type="GeneID" id="106543149"/>
    <dbReference type="KEGG" id="tsr:106543149"/>
    <dbReference type="OrthoDB" id="8015657at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026742">
        <property type="entry name" value="Centrosomal_kizuma"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16299">
        <property type="entry name" value="CENTROSOMAL PROTEIN KIZUNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16299:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN KIZUNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="276"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914543.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="43675" checksum="1536358DE45175CD" modified="2020-10-07" version="1">MMTGNVRGARDPTVAGCDEGDYDERIRRIQARLRHSESKRMELERKMLEYSTPDTSITKLKYTNLKKYLNEICERQKKSLLRNQDLLKEFDCIEAHIRKFASRSESLQMLKAAHEKEIKNRLLVERKDTLKQEMRDATEHQMVPEATQSGINARTAMSRGLYHTATIFMGRQMSAVSSVEDFSTLQKSEFKKSFSISDPHSYRQPSQSCYMTDSCVVQTNSDLQYSNKSDKIDGKTSLLKGEEMPVTSSVSYESEITSLKGESIANNDTDNFVENKVYPEHKPPLHSKLSSENRSTSSKSYNSCKSLTREHSLQYEQETKEPTSLIGDPGVLVSANEYSEEKSPVLESTMHLEKNILQEEDKSLSSSTDLTVSLSEEEEESNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWE1</accession>
    <name>A0A6I9XWE1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Renin receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ATPase H(+)-transporting lysosomal accessory protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">ATPase H(+)-transporting lysosomal-interacting protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Renin/prorenin receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ATP6AP2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Multifunctional protein which functions as a renin, prorenin cellular receptor and is involved in the assembly of the lysosomal proton-transporting V-type ATPase (V-ATPase) and the acidification of the endo-lysosomal system. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, may also play a role in the renin-angiotensin system (RAS). Through its function in V-type ATPase (v-ATPase) assembly and acidification of the lysosome it regulates protein degradation and may control different signaling pathways important for proper brain development, synapse morphology and synaptic transmission.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Interacts with renin. Accessory component of the multisubunit proton-transporting vacuolar (V)-ATPase protein pump. Interacts (via N-terminus) with ATP6AP1 (via N-terminus). Interacts with ATP6V0D1; ATP6V0D1 is a V-ATPase complex subunit and the interaction promotes V-ATPase complex assembly. Interacts with TMEM9; TMEM9 is a V-ATPase assembly regulator and the interaction induces the interaction with ATP6V0D1. Interacts with VMA21 (via N-terminus); VMA21 is a V-ATPase accessory component.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell projection</location>
            <location evidence="13">Dendritic spine membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasmic vesicle</location>
            <location evidence="15">Autophagosome membrane</location>
            <topology evidence="15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cytoplasmic vesicle</location>
            <location evidence="14">Clathrin-coated vesicle membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">Secretory vesicle</location>
            <location evidence="12">Synaptic vesicle membrane</location>
            <topology evidence="12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908860.1">
        <property type="nucleotide sequence ID" value="XM_014053385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWE1"/>
    <dbReference type="GeneID" id="106538771"/>
    <dbReference type="KEGG" id="tsr:106538771"/>
    <dbReference type="CTD" id="10159"/>
    <dbReference type="OrthoDB" id="7866065at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056780">
        <property type="entry name" value="Renin_r_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012493">
        <property type="entry name" value="Renin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057318">
        <property type="entry name" value="RENR_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13351">
        <property type="entry name" value="RENIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13351:SF1">
        <property type="entry name" value="RENIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07850">
        <property type="entry name" value="Renin_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25294">
        <property type="entry name" value="RENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 26" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026662705" description="Renin receptor" evidence="22">
        <location>
            <begin position="22"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Renin receptor N-terminal" evidence="24">
        <location>
            <begin position="21"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Renin receptor-like C-terminal transmembrane spanning segment" evidence="23">
        <location>
            <begin position="279"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF07850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF25294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013908860.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="39736" checksum="7BA8CCF7F582083D" modified="2020-10-07" version="1">MMAPRWGVLWVVSVVLAVVSANEFSVLRTPESVIFREGNWPIPGERISDVAALSMGFSIEEDLSWPGLAVGDLFRRPRATVLVTVTGIDKLTMPKNGISYPVENAVPFSLDGVANAIHTLFSEETPVVVQLAPSEERVYMVGKANSVFEDLSVTLRQLRNRLFQDNSILASLPLNSLSRNNEVDLLFLSELQVLHDITNLLSRHKHLAKDHSPDLYSLELSGLEEIGKRYGEDSQQFKDASQILADSLQKFADEMYNIYSGNAVVEVVTVKTFDTPFVRKSRSILQSSESKPENPYNLGYAYNFNYAVVFNIIFWIMLGLVLAVIVISYNLWNMDPGYDSIIYRMTNQKIRMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y356</accession>
    <name>A0A6I9Y356_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SLC52A2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>riboflavin(in) = riboflavin(out)</text>
            <dbReference type="Rhea" id="RHEA:35015"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the riboflavin transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917648.1">
        <property type="nucleotide sequence ID" value="XM_014062173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y356"/>
    <dbReference type="GeneID" id="106545568"/>
    <dbReference type="KEGG" id="tsr:106545568"/>
    <dbReference type="CTD" id="79581"/>
    <dbReference type="OrthoDB" id="9995836at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009357">
        <property type="entry name" value="Riboflavin_transptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52, RIBOFLAVIN TRANSPORTER, MEMBER 3-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06237">
        <property type="entry name" value="SLC52_ribofla_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013917648.1"/>
        </source>
    </evidence>
    <sequence length="424" mass="45207" checksum="30F5B41A27328017" modified="2020-10-07" version="1">MGLLVHLLVCLLGTGSWVAINGVWVELPLIVPRVPEGWLLPTYLTVIIQLANLGPLAFTLAHRFLRADEVGVIYGLLALGCLACLLLAFFWQETRPLGASRYSLALLVLLFFLALVDCTSSVTFLPYMRRLPSRHLTTYFVGEGLSGLLPGLVALGQGASAARCVNASLVGNGTTELLAEYQEAHFSIRTFFLLLSAMMALSLAAFLVLNHLPGLAKRYRARQGNPDDAAAAVAGHPMLRAPDSGCRAAPSRAQKACVFGLMAWANALTNGLLPPLQSYSCLPYGRPAYHLSAALGSLANPLACFLGMFLVSRSLRLLALLSGAGSLLACYIISMATLSPCPPLLHSPLGVLLIVICWVLFTGLFSYVKLMVGKILRDEGRSALVWCGAVVQLGSMVGALIIFPLVSVYGFFHSGDPCSSSCPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3A1</accession>
    <name>A0A6I9Y3A1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Epidermal growth factor receptor kinase substrate 8-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">EPS8L1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EPS8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921256.1">
        <property type="nucleotide sequence ID" value="XM_014065781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3A1"/>
    <dbReference type="GeneID" id="106548415"/>
    <dbReference type="KEGG" id="tsr:106548415"/>
    <dbReference type="CTD" id="54869"/>
    <dbReference type="OrthoDB" id="4680325at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900029">
        <property type="term" value="P:positive regulation of ruffle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01210">
        <property type="entry name" value="PTB_EPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09540">
        <property type="entry name" value="SAM_EPS8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11764">
        <property type="entry name" value="SH3_Eps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000023">
        <property type="entry name" value="Epidermal growth factor receptor kinase substrate 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000261">
        <property type="entry name" value="Epidermal growth factor receptor kinase substrate 8-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039801">
        <property type="entry name" value="EPS8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055093">
        <property type="entry name" value="EPS8_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033928">
        <property type="entry name" value="EPS8_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035462">
        <property type="entry name" value="Eps8_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287:SF19">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE 8-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE EPS8-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22975">
        <property type="entry name" value="EPS8_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PID" evidence="7">
        <location>
            <begin position="36"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="431"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013921256.1"/>
        </source>
    </evidence>
    <sequence length="672" mass="75653" checksum="73E592E84E94B64E" modified="2020-10-07" version="1">MSDSGSLFGDVHKPSARAIYEQRKKYSNFIVADVSQYAVNHLVTFSIGEEDDLTSVEDAIRKLCIMDKQGKIWVQEMLLQVNGSAIKLLDINSKEELENFALPTVAYCDVVCPESRARSLLVLMCQETTQLRPDIHFFDCEQIRAEWIQQDISSALLDFNTGGDIQRREALRAAQNMLDNQEVFSQPPMQVPSRTKIVAPGPREDAILIGEPDLSNIPIEQNVELLNRIFDDVEAFVRKLQKCAEAFRDLNQYRSSARGRLRTPREGLLTIRAKPPSQEEFEDALSKIKYSFSLLARLKPNISGPSSEELICILFKPLKMIVDASGGVEFASELRNPMLTLEAVTLMQSCLGEWETELWHSLGENWTKPRVDFPRGYAASYNLTFCSGWEPPNQDAFGRLWEDPVEMQNRHEERRNQQSAPTIAANGCKHLEKKSVICTHNFTARNSNELSVLQGDILEVLDDSKKWWKAQNCYGQVGYVPYNILAPMLNGDHSSIQNDKEIIVIHEINPIITEIPPPQPPAKTKNLQPDAVKWVSTEDLDVDDSKKDHFTMVNEELVLRLANGTGGGSKPLHIPRTLDTDAPLDYNSNSDQVQTWLEAKGFSPMIVSALGILNGAQLFSLKKDELRAINPEEGPRVYSQVTIQKALLKDSGKMSELEAVMARQKRKAEGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3Z4</accession>
    <name>A0A6I9Y3Z4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Receptor-interacting serine/threonine-protein kinase 4</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Ankyrin repeat domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">RIPK4</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Serine/threonine protein kinase. Required for embryonic skin development and correct skin homeostasis in adults, via phosphorylation of PKP1 and subsequent promotion of keratinocyte differentiation and cell adhesion. It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with PRKCB. Interacts with TRAF1, TRAF2, TRAF3 and TRAF5. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013911945.1">
        <property type="nucleotide sequence ID" value="XM_014056470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3Z4"/>
    <dbReference type="GeneID" id="106541128"/>
    <dbReference type="KEGG" id="tsr:106541128"/>
    <dbReference type="CTD" id="54101"/>
    <dbReference type="OrthoDB" id="195446at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14025">
        <property type="entry name" value="STKc_RIP4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000288">
        <property type="entry name" value="Receptor-interacting serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000260">
        <property type="entry name" value="Receptor-interacting serine/threonine-protein kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF65">
        <property type="entry name" value="RECEPTOR-INTERACTING SERINE_THREONINE-PROTEIN KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="13 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 29" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17 29" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="22"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="468"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="501"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="534"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="601"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="634"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="667"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="700"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="732"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="290"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="290"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00059729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00063777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00071067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013911945.1"/>
        </source>
    </evidence>
    <sequence length="788" mass="87820" checksum="18F61067877CB558" modified="2020-10-07" version="1">METEGRSSWAMGLLKTFDSSEFVSWEKIGSGGFGQVYKARHVQWKTCLAIKCSPSLHVDEKERMELLEEAKKMEMAKFRYILPVYGICKEPVGLVMEYMETGSLEKLLASEPLPWELRFRIIHETAVGMNFLHCMSPPLLHLDLKPANILLDAHYHVKISDFGLAKCNGLSHSHDLSMDGLCGTIAYLPPERIKEKNRYFDTTHDIYSFSIVIWGVLTQKKPFSEENNILHIMVKVVNGHRPELPPVSKSRPRACNNLIKLMQKCWQDDPSKRPTFKEITSETEELCEKPDYENKEMTVEEDAKVSQTYPKEMSSISQLKQEPDPASVKDYSLSELLSQLDSGISQTEQTEELSRSASESRLATSDKRLSGVSSVDSAFSSRGSLSLSFERENSGSDIGTTDIQKRKLAEAIISGDTSKLMKILQPQDVDLILDGNGGLLHLAIESGQEECVKWLLLNNANPNLTNRKSSTPLHIAVEKKNKSIIELLLTKKINVNAKDEDQWTALHFAAQNGDELITRMLLEKNALLNEVDFEGRAPIHVACQHGQETVVRIFLRKGIYVDVRGKDNWVPLHYAAWQGHLPIVKLLAKQPGVNVNAQTVDGRTPLHLAAQRGHYRVARILVQLQSDVNIRNAFFHTALHVAAETGHTSTSRLLLSRGADIEATTAEGCTALHLAARNGHLSTIKLLMEEGSNMLARGPLNRTALHLAAENGHDEVVKVLVNRENINDSDEEGLVALHLAAKGGHTQTAEVLLNHGACISMQNFAFQTPLQLAKQNGSNTIITLLSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4B0</accession>
    <name>A0A6I9Y4B0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lon protease homolog 2, peroxisomal-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912180.1">
        <property type="nucleotide sequence ID" value="XM_014056705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4B0"/>
    <dbReference type="GeneID" id="106541314"/>
    <dbReference type="KEGG" id="tsr:106541314"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="1">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912180.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="14665" checksum="B348806A6C27381F" modified="2020-10-07" version="1">MPQSMPEYALTRNYLELMVELPWSKNTKDCLDIRAARVLLDNDHYAMEKLKKRVLEYLAVRQLKNNLKGPILCFVGPPGVGKTSVGRSVAKTLGREFHRIALGGVCDQSDIRGHRYFKSLLLLLLLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5E8</accession>
    <name>A0A6I9Y5E8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of G-protein signaling 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RGS13</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
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            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913080.1">
        <property type="nucleotide sequence ID" value="XM_014057605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5E8"/>
    <dbReference type="GeneID" id="106542007"/>
    <dbReference type="KEGG" id="tsr:106542007"/>
    <dbReference type="CTD" id="6003"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF32">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="1">
        <location>
            <begin position="34"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913080.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="19831" checksum="BA80AFF1A45BAAA8" modified="2020-10-07" version="1">MNRSICWLCSLFRDGTNRVISKIHLEEVLQWSQSFEKLMATKYGPIVYKSYLKTEYSDENMEFWLACEKYKKILSERKRVSEAQKLFKEFIQPQAPKEINIDSPVREGITLKIQEPTQCCFDEAQKIVYLHMERDSYPRFLSSEIYQSLIVTLSAQQQSTYCLKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5G8</accession>
    <name>A0A6I9Y5G8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 28B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCDC28B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913130.1">
        <property type="nucleotide sequence ID" value="XM_014057655.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106542046"/>
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    <dbReference type="CTD" id="79140"/>
    <dbReference type="OrthoDB" id="9977011at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025271">
        <property type="entry name" value="CCDC28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13400">
        <property type="entry name" value="CHEMOKINE C-C MOTIF RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13400:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 28B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13270">
        <property type="entry name" value="CCDC28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913130.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="31486" checksum="AF16B82BCDA4E541" modified="2020-10-07" version="1">MQGFPKSSLGHHSVTFAWPPPFCLLFSCRLGGRERDPTMEERKKKSPKAHLSQPVPASALRKLPGPASKSASFSLGTPHLPSPRPRAKFKRASKETSRVPQLGGNAGSVVGTPLQHSFLTDVSDVYEMEGGLLNLLNDFHSGKLQAFGRECSFEQLEHVREMQEKLARLHFSLDVYVEELVEDQKKTVADRNLDQLLTNLEELSNSMYPFCRAQWGSPGKLVRRWERKKDVNGVPFQRTLWPMGRQCMGVHGDGGILCIGLHCALSWTPESKGQPISCLQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y686</accession>
    <name>A0A6I9Y686_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 247</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMEM247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920488.1">
        <property type="nucleotide sequence ID" value="XM_014065013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y686"/>
    <dbReference type="GeneID" id="106547760"/>
    <dbReference type="KEGG" id="tsr:106547760"/>
    <dbReference type="CTD" id="388946"/>
    <dbReference type="OrthoDB" id="9427125at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029200">
        <property type="entry name" value="TMEM247"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36691">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36691:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15444">
        <property type="entry name" value="TMEM247"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920488.1"/>
        </source>
    </evidence>
    <sequence length="425" mass="50004" checksum="121D107EB5DE0C26" modified="2020-10-07" version="1">MPVCPANLSVMTDNLANGDQQMLLETLQQIEIINCPLPLEAVVKKEKNVCKDDKYDYAEEQEQRKARHTQTHETDSEIERMRLDFELTVLKRQHEENDKQRLHEEKMEHIRQGSPSRSGAHMEEEQFGGKLDPITEIELEKLRMEFELAKLKHISEENERQRQHEQKLYEEKEKQRQHEEKMEQMRQRQGNLQIVSQRVSGCAPAEATAETASVAFAELGKMRIELQLALDVYRPEMKEKKLSGEQNGHQESPKCEQQLETTSEVKKADMPPEQQNDGVTEHLGLQVPRIVVNGAESQWSSSRLDLAIETELEKRRMEFELTRLRYEHEENERQRQHEQKMEQLRQQRQHKEKMEEMRLQAPSGQVSGGGHHFLLPHDPLTLFLYCFIFIHLIYIAKELIFFFFKKHGMFAIGAVLIFAIKIFWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y709</accession>
    <name>A0A6I9Y709_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">C3a anaphylatoxin chemotactic receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106543082</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914465.1">
        <property type="nucleotide sequence ID" value="XM_014058990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y709"/>
    <dbReference type="GeneID" id="106543082"/>
    <dbReference type="KEGG" id="tsr:106543082"/>
    <dbReference type="OrthoDB" id="10044919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF48">
        <property type="entry name" value="C3A ANAPHYLATOXIN CHEMOTACTIC RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="50"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914465.1"/>
        </source>
    </evidence>
    <sequence length="320" mass="36578" checksum="F8BE6B8ED4611BD8" modified="2020-10-07" version="1">MNSSLTALWLPNASWPSSLETVSDVNRFQVLSSSLKFVFYMFIFLLGSLGNGIVIWITCRQVSRTISCVWFFNLALADFLFSMSRIAPLVAVKKGWIFGSFACKANGLIKYLNMFCSEFLLAAISMDRAACIAYPLWARRHRNIRLVWLAAVVAWIMAIIISIPFYLYRKVDTKNNRTECRVMLKGEEPEVQVTIYMLRLICGFLIPFTIIVVCYGIIIMVLRRRQTSLHSKKSFKVILALVVTFSLCWLPYHITQLLTLPEVKGPTFLFIKLAAAFLAYLNSCANPFLYFFMGVDLHTKLTLYSIIMALRRTLLDESSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7W7</accession>
    <name>A0A6I9Y7W7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H/ACA ribonucleoprotein complex subunit DKC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dyskerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DKC1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915895.1">
        <property type="nucleotide sequence ID" value="XM_014060420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7W7"/>
    <dbReference type="GeneID" id="106544212"/>
    <dbReference type="KEGG" id="tsr:106544212"/>
    <dbReference type="CTD" id="1736"/>
    <dbReference type="OrthoDB" id="10250002at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="9">
        <location>
            <begin position="45"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="294"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="422"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013915895.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="59168" checksum="83DB04F670BD092E" modified="2020-10-07" version="1">MADGTDSVPKKHKKKKKSLPDEDVADIQHTEEFLIKPESRVAQLDTSQWPLLLKNFDKLNVRTAHYTPLPSGCNPLKREIAEYVRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIESEMQLSRAIETLTGALFQRPPLIAAVKRQLRVRTIYESKLVEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMGEKDHMVTMHDVLDAQWQYDNNKDETYLRRVIFPLEKLLVSHKRLVMKDSAVNAICYGAKIMLPGLLRYDDGIEVNQEIVVITTKGEAICIAIALMTTAVISTCDHGVVAKIKRVIMERDTYPRKWGLGPKASQKKMMIKQGLLDAHGKPNEKTPQTWKKDYIDYNRVSVKKETEEKTEPEEIQEQPERMPKKKRKIESDGDPEPVASAPPASPEEEQAEMKKKKKKKKEKRKMESAESSGELVEMVSDLSVKKKKKKKKREEIEESSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y900</accession>
    <name>A0A6I9Y900_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP:AMP phosphotransferase AK3, mitochondrial</fullName>
            <ecNumber evidence="8">2.7.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Adenylate kinase 3</fullName>
            <shortName evidence="8">AK 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">AK3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + AMP = GDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:29863"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:13749"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adenylate kinase family. AK3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.10" evidence="8"/>
    <dbReference type="RefSeq" id="XP_013917810.1">
        <property type="nucleotide sequence ID" value="XM_014062335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y900"/>
    <dbReference type="GeneID" id="106545693"/>
    <dbReference type="KEGG" id="tsr:106545693"/>
    <dbReference type="CTD" id="50808"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046899">
        <property type="term" value="F:nucleoside triphosphate adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046039">
        <property type="term" value="P:GTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046041">
        <property type="term" value="P:ITP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenylate_kinase_AK3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
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        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
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        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AK3/Ak4_mitochondrial"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="10">
        <location>
            <begin position="128"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="8">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="8">
        <location>
            <begin position="127"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="161"/>
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        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
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        <location>
            <position position="172"/>
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        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
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        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013917810.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="25636" checksum="4A77AF834445B18C" modified="2020-10-07" version="1">MAVRAALMRALIMGAPGSGKGTVSSRIVQHFALKHLSSGDILRDNMNKKTEIGILAKSYIEKGQLIPDDVMTQLMLSELKILDQNHVLLDGFPRTVPQAESLDKVCPIDTVIVLDVPFETIKQRLTARWIHPASGRVYNLEFNPPKISGHDDHTGDPLVQRDDDKPETIMKRLKSYEAETKPVLEYYRQKGVLESFSGTETNKIWPHVYRFLQTKLPEVSQKGTATES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC84</accession>
    <name>A0A6I9YC84_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tumor necrosis factor ligand superfamily member 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Osteoclast differentiation factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Osteoprotegerin ligand</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Receptor activator of nuclear factor kappa-B ligand</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">TNF-related activation-induced cytokine</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106548815</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tumor necrosis factor family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014066281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC84"/>
    <dbReference type="GeneID" id="106548815"/>
    <dbReference type="KEGG" id="tsr:106548815"/>
    <dbReference type="OrthoDB" id="8783336at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045672">
        <property type="term" value="P:positive regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00184">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000008">
        <property type="entry name" value="Tumor necrosis factor ligand superfamily member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471">
        <property type="entry name" value="TUMOR NECROSIS FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471:SF3">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0202">Cytokine</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="THD" evidence="20">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="22">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013921756.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="16398" checksum="86E458305E075943" modified="2020-10-07" version="1">NIFSSLGTGKVNLTSWHHDKGWANVLNMTFSDGRLIANHDGFYYLYANVCFRHHETLGNLTGQALQLMVSVIKTDARRRNSVVLMKGGSTKNWSGESEFHFFSVNVGGFFKLKSGEMVNIQVSYPSLLDHSPEGTYFGAFKIRDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCV7</accession>
    <name>A0A6I9YCV7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATPase family AAA domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ATAD5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921870.1">
        <property type="nucleotide sequence ID" value="XM_014066395.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106548897"/>
    <dbReference type="KEGG" id="tsr:106548897"/>
    <dbReference type="CTD" id="79915"/>
    <dbReference type="OrthoDB" id="9996895at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061860">
        <property type="term" value="F:DNA clamp unloader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000846">
        <property type="entry name" value="ATPase family AAA domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF21">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="908"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="976"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921870.1"/>
        </source>
    </evidence>
    <sequence length="1600" mass="180030" checksum="DA65BD69BFEDDF7B" modified="2020-10-07" version="1">MAVFAAATASPTDGAAQSCTKRKEDNISIKAITNYFSPLGKNVESVLSPPKSNNIMDYFKKTSINEKAPSPLLDQESKANVVSLENSSKPPSKLKSRGKWNKLNNKLKTDKTPEPLVEVNKDNDKETPNPQEENSKASFMNHNDIALIDLTNEEQALNVCGEGLLNKVSYKNEERKSKIIKSNTKKLKRRKPKNEMDLFENSIPETQTNDSHKETNKQIASVTESENKTVVLTLDPEPRAPVTSYNTVTVSFEDFLKSQGEDFEQIPRKQTPDDFVALDKTDVDQDTQDLPLKKVTVLAQIHSVPPKSLRSQKIASIFVNPKRKAKVKKSSASSEMEYIEQVTQKRKSNVVIAEEELELAVLESAGSETVKSKCKAEERYQFMKAFRQPGADVVKNGAKRGPSKQKDVEEKLASHKLEEEECEDESNQKPANNALMAYDSGPDHDLMKKCDRTRVRNKSKKRKGIVEAKEKAFNINDNQNNNAHGPINRTTPTEKEKEFSQKSKRLRRHCRQKRSQSTPEKAMVSQPVTENPPDACPLQTSTPKTKRSFKNDLYTAEVITEPFDSNSPIRMKFTRINTSDRSAHQNVKMNSTSSNIFKAKKLVEKAKAVQHNRISRTMEEAFHISLRRSSRQRALAEKKHLEKIENSQALDSGTVSDQIENPKKLQSVNDVLGKRTKFGKATKKSNGKEKMSLAIRKKNKKSTDAAVIVIKSSEDESENSQDYTEFKAKREFLMSGLPESLKKKIAKKAAVLEAYSAANSCFQKVIHVQQKDNGCLMWRLAPPSCRFFTRLKDVNSEVTDVAKLTLSLGELSVLTTQLNSNDSAVVVVNFAGEDTLWTEKYQPQNSSELIGNIEAIKKLHRWLCEWKRKASWEENRTQKEKKNGSKDDSSEHLDFVDDTSVSDEENVLCNTMLLIGPPGIGKTAAVYACAQELGFKIFEVNASCQRSGRQILSQLKEATQSHQVNKQGVHAHKPCFFNHYSSTKSPKKQSPKKEWSPRKFSGSPRKVGTKQSLMPKTLDNYFKLTSKLKNQEEKVPRSNKENRKSLTEKKPELKSGERRNKEEESSNKCATSLILFEEVDIIFDEDTGFLNAVKTFMSTTKRPVILTTNDPLFSETFDGCFEQITFRVPSLINVASYLQVLCLAENLRTDIRDFAALLTVNNCDIRQSILYLQFWVQSGGGYMKEKGNRTEIKGTQQNVSIEKSTSSNSRQLILEDIPKCSTGCTENLLGLKNIFLPSEDLFSFLKHEIGTKEDWNKLIHLLTEFQMRNTDLTYSNLEIISSLPLNIIPEAIEIASPACSFIAEKALSSTCIEIDCLEGAPPVKKAKRSKCQKKIAVLEDSDLFERELNYSGFVTLSPDTSASCLEQTGPAEPMNVGPVAPKDGTSVSAKDSVLVSQCLNSLTEYLDNMSLLDCCFTEPTQASKQASKYGDFIWTKGKIKNGLSDEFSVEHTDWWSSQSFCELKATMEALSFNKCSKSILKIMENCLNCTKTLGKNEFEELTLHVSKERADLYFGQSAANSSVHYNAQKRIDLIKNVFSSAPLSLNSRQASVMEYLPILRNICKSEKLKQQGKTKRRFLHYLEGIHLPKEILNSLATDFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDI2</accession>
    <name>A0A6I9YDI2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Regulator of nonsense transcripts 3B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">UPF3B</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RENT3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922212.1">
        <property type="nucleotide sequence ID" value="XM_014066737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDI2"/>
    <dbReference type="GeneID" id="106549163"/>
    <dbReference type="KEGG" id="tsr:106549163"/>
    <dbReference type="CTD" id="65109"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12728">
        <property type="entry name" value="RRM_like_Smg4_UPF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000067">
        <property type="entry name" value="regulator of nonsense transcripts 3A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034979">
        <property type="entry name" value="UPF3B_RRM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF1">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="UPF3" evidence="9">
        <location>
            <begin position="59"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="219"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="219"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="343"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="473"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922212.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="58404" checksum="6561647A27B45165" modified="2020-10-07" version="1">MKEDKENARPKEKRGGPLAPTGLGAAMAGADAKAGGEPDRLERPKDKKETLSKDETFFFKVVIRRLPPSLTKEQLEEHLQPLPEHDYFEFFSNDSSLYPHMFSRAYINFKNQEDIVLFRDRFDGYVFIDHKGLEYPAIVEFAPFQKAAKKKNKKKDAKAGTIDDDPEYKKFLESYSADDEKLTSTPETLLEEIEARNKELIAKKTTPLLNFLKNKQRLREEKREERRRRELERKRQREEERRKWKEEERRKRKEAEKTKKVERCPEKERDKSKDEPKIKLLKKPERDERDFEKKEKAKRLEKENLREDKIASPIGSVPAKRSDGEAKEEKTKKPVYPLQFEDECGKDYREREYDRDRDYERIQRDKLRRQEEDRRRQKERFEKEKVFRRKEDDVKRERDSLREKGKRADFMEYVGNPEKTEKGTKEDKRDDLAKKDRIRNKDRPAMQLYQPGARSRSRLSAYDESSPKSCDQGADKNPELEASNLKEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDZ6</accession>
    <name>A0A6I9YDZ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein KIAA2022 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">KIAA2022</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922386.1">
        <property type="nucleotide sequence ID" value="XM_014066911.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549304"/>
    <dbReference type="KEGG" id="tsr:106549304"/>
    <dbReference type="CTD" id="340533"/>
    <dbReference type="OrthoDB" id="9878678at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033629">
        <property type="term" value="P:negative regulation of cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000048">
        <property type="term" value="P:negative regulation of cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001953">
        <property type="term" value="P:negative regulation of cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001223">
        <property type="term" value="P:negative regulation of neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032757">
        <property type="entry name" value="DUF4683"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042794">
        <property type="entry name" value="Nexmif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46946">
        <property type="entry name" value="NEURITE EXTENSION AND MIGRATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46946:SF1">
        <property type="entry name" value="NEURITE EXTENSION AND MIGRATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15735">
        <property type="entry name" value="DUF4683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4683" evidence="4">
        <location>
            <begin position="284"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="348"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="739"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1148"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1356"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1426"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1148"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1441"/>
            <end position="1461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013922386.1"/>
        </source>
    </evidence>
    <sequence length="1471" mass="160794" checksum="D142112872BC065E" modified="2020-10-07" version="1">MDDQQDKECASEDQEIILINGVKEDESSHLDGDEKPLRGAADAAAPFLGVTSTGAPQKESRSQPAPTSKKACWLSPPSPLRLADVPDHISDDSSSIHAISLTSCVTKGMSAWSLPGDCEKAPFTIMEPGSMSAFTGDCLMQPSRTCLGCFIESKDSLDPEPGISLKVGDINRDYDSCSVSDIGIHCMSTGETMRYGDQLLSDQLLSFPVHKSRAADKREAEKSDSDSEDPTQKNYYEGLLLDKCNGEEPLLTNPNQEWGYFESFISESKIELLDLCSKNELSVNLFSEEDVDNYMFDDDDSTLESDVCSLKIRYESFQDNVKEKTGGLQEDTQFNFFPSVFSGCPKRDNRTALKRGPRGATEPSQFKSEETIIWGEEEGDGQQEEEEEEEEEDEEGGESEKVALNKSCTSTELVQYISPKRNHFLELVNSTEDSGESSDYSTCTESSFDVLQDLKDCNKYLSRDHSTSFIQQNYGLRAKRKVRYSEDYLYDVDSIESEKIVDKKEWIPDGPKEEDDDDWCPKKRRKVSRKEPPVIIKYIIINRFKGEKHMLIKLGKVDPSEMTVTLSEELLSKYAQLAPLKSFWQERWQTRLNFLKSALYHKQSFYLNGSEVAFLSHPRKRKGKIAHRHRIQRIKAIEQPVGKAGSCSSNSKQLCCRITGEDGGLKGIQAMAITAPSCVNGLHLSDIAGLALPRSKAQEREFKGPERKVLRRIKFKSEARLKCKRLKAASALAEVSAALEDSESATGSKDESGPCTADGSPLSESQEDKQAKNALSFLPTTCTSDKPATSATLAASVPLIPGGYLQTLLDASDLSHSTGIAYFSPAPSEQQPQQETLPSLVQAEKAFCVLQPAQSCILSPPSESELQQSPSPLEIEPSNFQSVWPAGKAGSPSSQGFAGQLPDASLLSAVEFNNCAGSDGLPASGYSQVHLSNSKVPHQKKFLLEGQQLQSDDSYQSCPFGNREGNFPFQRGSLSTDDGRLVSFDSVGSLSATSSNYSSLSLKSCEKDGDDEIADDFLAHCSPKLVIQQSIDEITPLKESTDLLDISNFTPDKFRQSSLSEMSPPDTPNLSPQIAGPEAKTLLGNMKCFPEGSQVGLSNTEKVNWSCGVLQAPDQADNGFTLNNHQFQFHMFTDEDSVSLLEKNSSLSTFNEPSAQVSTNSKATKSKRRSSSNKNLGANQSPTPKNVRKKSPKANKSSEKAPSRNSRQSPKTSRKGKNGAGVNGEKALGDRVPGLLNSANSSAKMLVESIQQGSPNALRIGKANGEWPLGKDSSGGWSETSIGSANSLLDDDQREFEEPSNILSNIASGMADVQRFMMASIEPLWGPVGHSDVSDLFRSPESNSLKLKTLKILAGTSPESKKKVNGSSSGAGRNHKSGNKGLSKTGSRAAPGDPGRSSSSAGYAADIHSPFFDKSYSNLSTLAKNEPTHKKLYRHKSTSKSLRDENCKVKRAEREQGHKDPTTVVSFEKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGG9</accession>
    <name>A0A6I9YGG9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cartilage intermediate layer protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CILP2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923099.1">
        <property type="nucleotide sequence ID" value="XM_014067624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGG9"/>
    <dbReference type="GeneID" id="106549867"/>
    <dbReference type="KEGG" id="tsr:106549867"/>
    <dbReference type="CTD" id="148113"/>
    <dbReference type="OrthoDB" id="9929167at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056257">
        <property type="entry name" value="CILP-1/2_8th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056256">
        <property type="entry name" value="CILP-1/2_b-sand_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056258">
        <property type="entry name" value="CILP-1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056255">
        <property type="entry name" value="CILP-1/2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039675">
        <property type="entry name" value="CILP1/CILP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025155">
        <property type="entry name" value="WxxW_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031:SF0">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23591">
        <property type="entry name" value="CILP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23708">
        <property type="entry name" value="CILP_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23730">
        <property type="entry name" value="CILP_8th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23599">
        <property type="entry name" value="CILP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13330">
        <property type="entry name" value="Mucin2_WxxW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1195"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="236"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013923099.1"/>
        </source>
    </evidence>
    <sequence length="1250" mass="140252" checksum="A806412003381327" modified="2020-10-07" version="1">MYPSVKWASFWMACCGKPCCRVATLGNTSKSHGSLFPPLLPAGEWTSWFNVDHPGGEGDFETLEAIRFYYPERICARPLSIQARTTKWELPKEVGEVVRFSPQEGFHCLNKEQLSNRTCSNYHVRFLCPVACQLSCAIGIPNKDCTGCICPNHTLLGTVRRADGMPLADVPIALADQPLTPLAQSNRWGQFTIRGICAGDSTNVTAKLERFAPGSASPVVNGSGTSLVEITLHRLEQLYMVTSPESKVRMVGEKVRFCCSAQGTPEPQRYYWYHNGMLLNWKTYNYSRSLVLRDLRTHQAGFYHCKASNNHSSIKSSAAHLTVISPDVPACRAQPEEYLIKLPDDCFQEATGSNFYNVGHCPSTPCAGNSSDGLRCQDATQHCCGTQRMEVREIHCTGYILPVKVVAECGCTTCTRPKVLVRGKASAADDGEPLRFGEIYLGAEKIGFTGYQGTFTIEVPPNTERLVVKFVDRLQKFVDAIKVFPLDQRGGAVYQEVKLMRKKKPVDLASMESNTIPLGEVSGEHPIGEIVIPPGSFFRSDGQVYNGTVKASITFVDPRDIGTVSTASSDLNFINAEGDLFPLRTYGMFSMDFWEEETHQGLEVGRAEIKMDTDLIKTLEHTEKMKLWSLNPITGLWEEEASLKLAKKKRKKREERNFLIGNLEVRERRLFNLDVAERRRCFVKVRTYINEKFYPSEQLEGVIVTLVNLEPRPGYPSNPRAWGRFDSVVTGPNGACLPAFCDSERADAYTAYVTATMGGEELEAVPSSPKLNPRAIGVSQPYLNKLGYQRTDHEDPALKKNAFRINLAKPNQNNIDETNGPIYAYRNLKECDQAPVTANHFRFYRVEEDKYEYNVVPFKETDLTTWTGDYLSWWPNPQEFRACYIKVKINGPHEYMVRSRNVGGSHPRTRGQLYGLRDSRSIRDVDILNSSAACVEFKCSGMLFDQGMVDRTVVSVIPQGSCHRTAVNSYLREYLSRHPPLVENNDTMAFSMLAPVDPLGHNYGIYTVTDQNPHLAKEIAIGRCFAGASDGFSREMKSDRGTALTFTCYEKPVGTKSFFQRLLESPSQTLTEMRREMSANEQLRGPSQVVSYPSGLRTVLQRRPSRRRRVGTSPIQQQGLEKQYSSYASFSEAGHLRVLLPSAAFPLEILEFLIVRRLHDAPPSSWNVTEASRSVAGASPFQTFFTPYLEQARQIWPWLLGAAVIGAIIAMVVKGLITLGTRKKKRSMEETQPLLMEDENYPHLTYQSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHX3</accession>
    <name>A0A6I9YHX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein SFI1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923786.1">
        <property type="nucleotide sequence ID" value="XM_014068311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHX3"/>
    <dbReference type="GeneID" id="106550407"/>
    <dbReference type="KEGG" id="tsr:106550407"/>
    <dbReference type="OrthoDB" id="195843at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052270">
        <property type="entry name" value="CACF_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028:SF4">
        <property type="entry name" value="PROTEIN SFI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923786.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="49606" checksum="7EEBC94EDB1EAEA8" modified="2020-10-07" version="1">MLLQRQGEQLLARRLSAAAFAWWKVQLADRRQEQHQTTRALWHWSQSLQQKVLNAWRGFMQEQLRKKRRMAWAAESYQAELLREGACRILRYVVAVKQHRECLQAQHHLQAACQRHHLVYRCAMIWKQKALSRKPSLALSGIPAKKHVTFEVPRLGHVPPGVPRRQANLPGVPRDCQAAGDSILSDLYTARQVRLQPRRPDFLVPFPEKRGLPHAGKWLESSAGFAQPMAAGPLLPVPPSIGPSPSPFSGCGDSSGSFPKPGPPPPRSPWLHSPTSAAPELQPPSSFTLGTKRASVETPSLSPLAAAPGKERPMVVSRGPLLAPKDFTRGRRSPPLCGKAETKRGKTEIQPLEDELQCIGQKMQDYYSQQQELKFCQRQERLLSKWLELRGVGEPSDAQEIREKLGQLKVQIASLVSTLEGERQLMQKYMSRVQAIRAALKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI31</accession>
    <name>A0A6I9YI31_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kininogen-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923861.1">
        <property type="nucleotide sequence ID" value="XM_014068386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI31"/>
    <dbReference type="GeneID" id="106550463"/>
    <dbReference type="KEGG" id="tsr:106550463"/>
    <dbReference type="OrthoDB" id="9937817at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923861.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20629" checksum="DF6A500775A8FA3F" modified="2020-10-07" version="1">MRPWVRLQVPEVTCSETQISQNYLRVPPGFTPFRSALPVANDPEGPRVVEEFHIDPRGRRAHEKKQGHGHRHRHGHRHKHKKSKESSSEEVAVLPAIETPSQLPTSKAFLEQEANSRSPHGEPVGFPDASPVHSSSTDSPVFLHHLPDLPEPPKCPGTPWKPERAESEDTLFNDFDLYDALQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YII4</accession>
    <name>A0A6I9YII4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Uncharacterized protein LOC106550749</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">LOC106550749</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM176 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924183.1">
        <property type="nucleotide sequence ID" value="XM_014068708.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924184.1">
        <property type="nucleotide sequence ID" value="XM_014068709.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924185.1">
        <property type="nucleotide sequence ID" value="XM_014068710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YII4"/>
    <dbReference type="GeneID" id="106550749"/>
    <dbReference type="KEGG" id="tsr:106550749"/>
    <dbReference type="OrthoDB" id="8951938at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009281">
        <property type="entry name" value="TMEM176A/TMEM176B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15756">
        <property type="entry name" value="LR8/HCA112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15756:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 176A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013924185.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="29980" checksum="D9F8C7B5B6620A46" modified="2020-10-07" version="1">MAGTVLKIAGSEAALGGSGGTVVNITLRQESGMAYLCKGLASLGRRKAAAKDATGAPPARHPHGGEQKVLGGAQILLGAVCIGLGVILSLTPNPAYIDYYYYRRVLDNGVPFWTGSLFILSGSFSVVGARHGGNWVHLATFLNLGSVVAGSVAFALGLAEFPSLTYQPLFVESLCKHRAEERGYESWGLTASPSYDSQREHERARECQEALWAFLNMWSSSQILLLILHSAALAIALFCFGYGLLRLCRSCWGGWKDYMAVEEADTPPAPEEPGKEQNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJG7</accession>
    <name>A0A6I9YJG7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hematological and neurological expressed 1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">HN1L</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JUPITER family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924392.1">
        <property type="nucleotide sequence ID" value="XM_014068917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJG7"/>
    <dbReference type="GeneID" id="106550929"/>
    <dbReference type="KEGG" id="tsr:106550929"/>
    <dbReference type="CTD" id="90861"/>
    <dbReference type="OrthoDB" id="6367565at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930">
        <property type="entry name" value="GEO05313P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930:SF5">
        <property type="entry name" value="JUPITER MICROTUBULE ASSOCIATED HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17054">
        <property type="entry name" value="JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="109"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924392.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="20532" checksum="4CE14FEC8DC36704" modified="2020-10-07" version="1">MFQGPESDPSRSSSRVLQPPGGQSSDLFGSAEVSSSSKTQKMASNIFAAAEEPMNFPKRTNPPGGKDSGIFEDHKPNASRQFINPPGGKTSNIFGTPSPLSAVKAHPNKPKDHTFLFKDRGDSPRETKVPEDQRPQREELSEKKDLPKEIPKDPGPKIDDHEPRLGPRPRSHNKVLNPPGGKSSIAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJY0</accession>
    <name>A0A6I9YJY0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="16">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24 25 26 27" type="primary">WWP1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24 25 26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 16">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="16"/>
    <dbReference type="RefSeq" id="XP_013924678.1">
        <property type="nucleotide sequence ID" value="XM_014069203.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924679.1">
        <property type="nucleotide sequence ID" value="XM_014069204.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924680.1">
        <property type="nucleotide sequence ID" value="XM_014069205.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924681.1">
        <property type="nucleotide sequence ID" value="XM_014069206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJY0"/>
    <dbReference type="GeneID" id="106551170"/>
    <dbReference type="KEGG" id="tsr:106551170"/>
    <dbReference type="CTD" id="11059"/>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04021">
        <property type="entry name" value="C2_E3_ubiquitin_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000122">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase HACE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000093">
        <property type="entry name" value="NEDD4-like E3 ubiquitin-protein ligase WWP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF299">
        <property type="entry name" value="NEDD4-LIKE E3 UBIQUITIN-PROTEIN LIGASE WWP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11 16 18" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="20">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="21">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="21">
        <location>
            <begin position="378"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="21">
        <location>
            <begin position="453"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="21">
        <location>
            <begin position="493"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="22">
        <location>
            <begin position="584"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="202"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="251"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="258"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="17 18">
        <location>
            <position position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013924678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013924679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013924680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013924681.1"/>
        </source>
    </evidence>
    <sequence length="918" mass="105031" checksum="629E161013B28901" modified="2020-10-07" version="1">MAAASPTSGSSNNFDGRLQMQVTISSAKLKRKKNFFGLAAIYAELVADNEVKKTSKSGSSSNPKWEEQLTVNVTPQTTLEFRVWSHHTLKADALLGKATIDLRQALEVHNRRLEHVKEHLKLSLENKSGLIQTGELIIVLDGLVLETESLSNRSSLVTIEVQQNGDALHENRETPARTTSRLDNQTPSSSIVQISFPIHAVNGDNGDNSSSLHIVTRSKNSPIPKPLSSEQDNNIVSCESIMEADNLSISDIPMDSTEAPSTSADCTTTASETPIETEATTSNAESSTSTTAFNEFSTQPVVQPTPSDSVSSSTENNATPMTEVAKPSANVSGEAIRQQPGNTSVEPLPPGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLSAENDPLGPLPPGWERRVDSNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFNDPRTGKLSANKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLNKKLTIKDLESIDTEFYNSLIWIRDNNIEECNLEMYFCVDMELLGKVTSHELKSGGSNLLVTEENKEEYIGLMAEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDMADWQRNTVYRHYTRNSKQIMWFWQFIKEADNEVRMRLMQFVTGTCRLPLGGFAELMGSNGPQKFCVEKVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJZ3</accession>
    <name>A0A6I9YJZ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ubiquitin carboxyl-terminal hydrolase 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">USP25</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924481.1">
        <property type="nucleotide sequence ID" value="XM_014069006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJZ3"/>
    <dbReference type="GeneID" id="106551008"/>
    <dbReference type="KEGG" id="tsr:106551008"/>
    <dbReference type="CTD" id="29761"/>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02665">
        <property type="entry name" value="Peptidase_C19I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20486">
        <property type="entry name" value="USP25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF666">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013924481.1"/>
        </source>
    </evidence>
    <sequence length="793" mass="92042" checksum="C4A63A04F85A66C3" modified="2020-10-07" version="1">MFGQYPLQVNGFKDLHECLEAAMVEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPPVLYLDRYMHKNRETTRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPIEDLSTSAVPSGPLSTHAIPSTVEQQGPSTETQSAAPTQRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRKEVENDTRDLQESIARIQRTIELMYSDKSMLQVPYRLHAVLVHEGQANAGHYWAYIFDSYQQRWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEPLFIQEEFNKDTGHMIFGLDTLPPDLRDFVKEDNKRFEKELEEWDAQLSLKAQQEKISPVPRVPMSSTTQEPEYLEQPSTADFSKQLKEDSIRAITKAFTEQEDKNPETIMHMNSHNDPIGTAPNQKVVEVTIPDIGTYVVESEEGGYDDEVMLTPNMQGIIMAIGKATHIYDRCGPEAGFFQAIKFEYARLLKLAQEDTPPERDYRLHHAIVYFIQNQAPKKIIERTLLEQFADHNLSFDERCRNVMKVAQAKLQMIKPDEVNMEDYEWWHQEYINFRETTIYLMVGLEHFQKRNFKEALLYLICAYHKNKELSANGLYRGHDEELISHYRRECLLKLNECAAAQFESGDDQQVNKGLEIMNELIVPCLPLLLVDETEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFNDSPKLPSYSTNELCERFARIMLSLSRTPADGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK12</accession>
    <name>A0A6I9YK12_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endoplasmic reticulum membrane-associated RNA degradation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ERMARD</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924354.1">
        <property type="nucleotide sequence ID" value="XM_014068879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YK12"/>
    <dbReference type="GeneID" id="106550892"/>
    <dbReference type="KEGG" id="tsr:106550892"/>
    <dbReference type="CTD" id="55780"/>
    <dbReference type="OrthoDB" id="49386at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025209">
        <property type="entry name" value="DUF4209"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039635">
        <property type="entry name" value="ERMARD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31701">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE-ASSOCIATED RNA DEGRADATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31701:SF2">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE-ASSOCIATED RNA DEGRADATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13910">
        <property type="entry name" value="DUF4209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4209" evidence="1">
        <location>
            <begin position="114"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924354.1"/>
        </source>
    </evidence>
    <sequence length="343" mass="38624" checksum="8794137365862F7D" modified="2020-10-07" version="1">MICEAGFERKTSCAVSSFICDSGEINWEFITKHVQYGEQDSILDYINSMRSLGPLCESIHLHLKSLTVEQFENQFVVWLQWTNCPEIFLEMIDTIKNPHGAAVALSLMKLTSCLERALGDVFLLIGKDCPFLLRDLLASPELVSIFGQPVMDVLKVFIGSPDSLNLRNILWHGFVSVEEIPVKYFSMLLFLTAGLGQLLNNYCLQAHSALIHRPYVSFTHLKELHIFPDLNQELLSLAKELVTKSNIVLKTMIPFWIAAITSFQQARYADCVILLLPQLEGGLRVLFTAVNKCPSRLMTAESSSLYTTFDEILAKQLNNEEINQLPIVLGESAMSSADFHKMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKQ2</accession>
    <name>A0A6I9YKQ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pleckstrin homology domain-containing family B member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PLEKHB2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924731.1">
        <property type="nucleotide sequence ID" value="XM_014069256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKQ2"/>
    <dbReference type="GeneID" id="106551207"/>
    <dbReference type="KEGG" id="tsr:106551207"/>
    <dbReference type="CTD" id="55041"/>
    <dbReference type="OrthoDB" id="2157866at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13265">
        <property type="entry name" value="PH_evt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000073">
        <property type="entry name" value="Pleckstrin homology domain-containing family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039680">
        <property type="entry name" value="PLEKHB1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309:SF8">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY B MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924731.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24863" checksum="0DBE2BA1575B3FD9" modified="2020-10-07" version="1">MAMVKSGWLLRQSTILRRWKKNWFDLWSDGQLLFYDDQHRHDLEDRVHMKIDSINIRGGNECRGFQPPEGKAKDCLLQIICRDGKVLNLCAESTDDCLAWKIALQDARTNETYVASEVMYDDNAISSAPPPYTAYATPSPEVYGYPQYNGAYPGTQIIYTSNGQTYAMPYQYPNQGTYGPLPANHVIIRERYREPDGDLALGMLAGAATGMALGSLFWVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKZ8</accession>
    <name>A0A6I9YKZ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear speckle splicing regulatory protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 55</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NSRP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSRP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924709.1">
        <property type="nucleotide sequence ID" value="XM_014069234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKZ8"/>
    <dbReference type="GeneID" id="106551191"/>
    <dbReference type="KEGG" id="tsr:106551191"/>
    <dbReference type="CTD" id="84081"/>
    <dbReference type="OrthoDB" id="9048633at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042816">
        <property type="entry name" value="Nsrp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018612">
        <property type="entry name" value="NSRP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31938">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31938:SF4">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09745">
        <property type="entry name" value="NSRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear speckle splicing regulatory protein 1 N-terminal" evidence="6">
        <location>
            <begin position="28"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013924709.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="51962" checksum="D7ADF9900FE6E361" modified="2020-10-07" version="1">MDDSDEESSVGESLQKEALKKQAMKQTKLEIQRALAEDSTVYEYDNIYEDIQQKKAESQASSLAGKAEKKPKYIQNILQAAELRKKEQERRMEKKIQKERESEGDAFQDKEAFVTSAYKKKLQERAEEEQREKKEAAIEASLDVTKQKDLSGFYRHLLNQTVGEEKVPHCSLRGAGFKGEDPAEKPTGSHQNLESKTGTCVPGKGEENLDADSDSRSHSSDGEETVAEGVPERRGEQRASGAGDGDLRGRQSKQSPRDSRSPSKERPPRSPPAEQRDRQRDEAHQSDDRNSQRRHGKDERSRSDGHHRRREDREDRQKRKDWEEEEEEKREQRRGRDKKEHRAREKDRDGNREKGRGRDSDRDEKTRRKHSSGSVAQEDPQTQGGNLPSPEKSAEVTHKASEDSRETLEKPAEKVNKFAKRSNQETVSSARDRYLSRQMARVGPKAYVEKEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN52</accession>
    <name>A0A6I9YN52_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RNA exonuclease 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106551947</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925586.1">
        <property type="nucleotide sequence ID" value="XM_014070111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN52"/>
    <dbReference type="GeneID" id="106551947"/>
    <dbReference type="KEGG" id="tsr:106551947"/>
    <dbReference type="OrthoDB" id="206335at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000021">
        <property type="entry name" value="RNA exonuclease 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031736">
        <property type="entry name" value="REXO1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF62">
        <property type="entry name" value="RNA EXONUCLEASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15870">
        <property type="entry name" value="EloA-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="496"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013925586.1"/>
        </source>
    </evidence>
    <sequence length="658" mass="73479" checksum="758D6B7CE5D8B691" modified="2020-10-07" version="1">MLQMSMQSLSSSSSSSSDDDEVDYSHLENDLDFESDPMEECLRIFNESTDVKTEDKGRMGKQMSKEEGSEEKLTEDSLTTLFPGQKKRISHVTKQGHVEVPSKPVVRPYRPPTAQEVCYQRIQLAQKQAAQLAQQRPKQPLPPPVKIPVAPQRTSIVMHKGERKRVAHVPNAALSAVSKLAPGNLKKTIPPARVASSNGVDASALKPRTLMGMASKTTTTGLPKRIAHTPTLQSTTLKRPVIPTEYGAKVPTNIRQRYLNLFIDECSKVCPSQQAAFDKALAEEKVVYERSTSRNIYLNVAVNTLKKLRTLLPSPSQEVQKFSNRKVVSHEAMLGGKLAARTSYTLNRSTSLRLEDLTGATLYRRLKEYVLTEDDLKEHGYPLPCPERPGRAVVFAAEEKKLADGSCRICCRCGTEYMVSPSGNCVRKEECIHHWGRLRKQRVPGGWETHYSCCSGAVGSLGCQVAKQHVHDGRKESLEGFVKTFEKLTRADGHPGIYALDCEMCYTKQGLELTRITVINSDLRVVYDTFVKPDSKVVDYNTRFSGVTEADLENASITLRDVQAVLLSMFSTDTILIGHSLESDLFALKLIHSMVVDTAVVFPHRLGLPYKRALRTLMADYLKRIIQDSVEGHDSSEDARACMELMIWKIKEDAKVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP82</accession>
    <name>A0A6I9YP82_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Myoferlin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106552523</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926298.1">
        <property type="nucleotide sequence ID" value="XM_014070823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP82"/>
    <dbReference type="GeneID" id="106552523"/>
    <dbReference type="KEGG" id="tsr:106552523"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04017">
        <property type="entry name" value="C2D_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000026">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037723">
        <property type="entry name" value="C2D_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF55">
        <property type="entry name" value="MYOFERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926298.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="23393" checksum="53D8AC6A4ECA2E54" modified="2020-10-07" version="1">MSPFPCCTLSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEIEIYGDPQTVAQNPPQVVVDLFDNDQVGKDEFLGRTSCSPMVKLNPDIDINPKLLWYPVKNGGKACGDVLLAAELILNEKGGTNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGVMVESVVIKNLKKTPNFPGSVLFMKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPF1</accession>
    <name>A0A6I9YPF1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC106552317</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106552317</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926036.1">
        <property type="nucleotide sequence ID" value="XM_014070561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPF1"/>
    <dbReference type="GeneID" id="106552317"/>
    <dbReference type="KEGG" id="tsr:106552317"/>
    <dbReference type="OrthoDB" id="8908372at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026886071" evidence="2">
        <location>
            <begin position="21"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="20"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="167"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013926036.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="30351" checksum="68028636B9697574" modified="2020-10-07" version="1">MAQPFLFLLFLLSFFTGSLAQFTLTQPPSVSVSSGGTVRITCTRAGGSFSDHYVYWYQQKPGSKPLAVIIEDSERPSGIPERFSGSVDLSSKTATLSISNVQPEDEADYYCFSGENNQCTLNLSMTRRGPPRRNVGFRRSVLAHFSAMAQPFLFLLFLLTFFSGALAQFTMTQPPSVSVSPGGTVRITCTRTGGSFSDYYVSWYQQKPGTKPLAVIYKDSERPSGIPERFSDSYDSSSNSATLTISNVQPEDEADYYCLTYDGNNQCTVIALDGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPK6</accession>
    <name>A0A6I9YPK6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Myc box-dependent-interacting protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Amphiphysin II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Amphiphysin-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Bridging integrator 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">BIN1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <location evidence="16">Sarcolemma</location>
            <location evidence="16">T-tubule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926086.1">
        <property type="nucleotide sequence ID" value="XM_014070611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPK6"/>
    <dbReference type="GeneID" id="106552363"/>
    <dbReference type="KEGG" id="tsr:106552363"/>
    <dbReference type="CTD" id="274"/>
    <dbReference type="OrthoDB" id="446293at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048156">
        <property type="term" value="F:tau protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12139">
        <property type="entry name" value="SH3_Bin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000013">
        <property type="entry name" value="Amphiphysin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000029">
        <property type="entry name" value="myc box-dependent-interacting protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003005">
        <property type="entry name" value="Amphiphysin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035471">
        <property type="entry name" value="Amphiphysin-2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003023">
        <property type="entry name" value="Amphiphysin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46514">
        <property type="entry name" value="AMPHIPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46514:SF4">
        <property type="entry name" value="MYC BOX-DEPENDENT-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01251">
        <property type="entry name" value="AMPHIPHYSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01253">
        <property type="entry name" value="AMPHIPHYSIN2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="25">
        <location>
            <begin position="28"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="24">
        <location>
            <begin position="398"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="274"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013926086.1"/>
        </source>
    </evidence>
    <sequence length="471" mass="52121" checksum="E7D3B7CEC0013D79" modified="2020-10-07" version="1">MAEMGKGVTAGKIASNMQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLSEGSKLQKDLRTYLTSVKAMHEASKRLTECLQEVYEPEWPGRDDTNKIAENNELLWTDFHQKLVDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKPVSLLEKAAPQWCQGKLQAHLVAQTNLLRNQAEEELVKAQKVFEEMNIDLQEELPSLWNSRVGFYVNTFQSIAGLEENFHKEMGKLNQNLNDVLVTLEKEHATSTYPVKAQPSDSTKANKTPSSPPDGTPITSPEIKAVNHEPEPSTLEAPGGGIPKSPSQSTEATPAEADGAGKGGTAGAEAATKPETDSGSASSSLPTVVVDSATVNGTDENRAISERSDMPPGFLFKVQAMHDYAATDNDELQLKAGDVVLVTPFENPDEQDEGWLMGVKETDWLQNKAFDQCRGVFPENFTERVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPR7</accession>
    <name>A0A6I9YPR7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">YBEY</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925904.1">
        <property type="nucleotide sequence ID" value="XM_014070429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPR7"/>
    <dbReference type="GeneID" id="106552210"/>
    <dbReference type="KEGG" id="tsr:106552210"/>
    <dbReference type="CTD" id="54059"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00043">
        <property type="entry name" value="rRNA maturation RNase YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="ENDORIBONUCLEASE YBEY, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986:SF1">
        <property type="entry name" value="ENDORIBONUCLEASE YBEY, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925904.1"/>
        </source>
    </evidence>
    <sequence length="164" mass="19345" checksum="663A278B8C6E236D" modified="2020-10-07" version="1">MSLIIRNLQRAIPIRRVPLRKNIEQLRYILGVQKFDLGVICVSNMYIQQINRTYRQKNIPTDVLSFPFHENLKGGELPQPCFQNDNLGDIFLGVEYIYQQCRQNHDDYNSILTVTVAHGMCHLLGYKHNTEKDWQQMYEKETQILHELSKLTGTNFEPLSKHHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPS4</accession>
    <name>A0A6I9YPS4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PAM16</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925685.1">
        <property type="nucleotide sequence ID" value="XM_014070210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPS4"/>
    <dbReference type="GeneID" id="106552024"/>
    <dbReference type="KEGG" id="tsr:106552024"/>
    <dbReference type="CTD" id="51025"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925685.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="16507" checksum="C4D3B241342470D8" modified="2020-10-07" version="1">MAASGTKGRSFRSRSCGRPELRSAPDMAKYLVQIVLVGAQVVGRAFARALRQEFAASQAAADTRGRTGGQSWAASSLSGISLQEAQQILNVSTLSPEEIQKNYEHLFKVNDKSVGGSFYLQSKVVRAKERLEEELRIQSPREEDSPHKPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ38</accession>
    <name>A0A6I9YQ38_9SAUR</name>
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        <submittedName>
            <fullName evidence="11">Microtubule-actin cross-linking factor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MACF1</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
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    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_014071153.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:keratin filament"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:keratin filament binding"/>
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        <property type="term" value="F:structural molecule activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="SPEC"/>
        <property type="match status" value="7"/>
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        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
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        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
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        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X9"/>
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        <property type="entry name" value="MICROTUBULE-ACTIN CROSS-LINKING FACTOR 1, ISOFORMS 1_2_3_4_5"/>
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        <property type="entry name" value="DELLA"/>
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    </dbReference>
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        <property type="entry name" value="PLEC"/>
        <property type="match status" value="16"/>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="203"/>
            <end position="260"/>
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        <location>
            <begin position="2084"/>
            <end position="2111"/>
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            <begin position="2356"/>
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        <location>
            <begin position="2535"/>
            <end position="2560"/>
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    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="4227"/>
            <end position="4289"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2092"/>
            <end position="2103"/>
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    </feature>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="4528" mass="512038" checksum="E2071265D5FBDC8A" modified="2020-10-07" version="1">[encoded data, 4528 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRC4</accession>
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        <submittedName>
            <fullName evidence="3">Telomere repeats-binding bouquet formation protein 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">CCDC79</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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            <taxon>Serpentes</taxon>
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                <consortium name="RefSeq"/>
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        </source>
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        <property type="component" value="Unplaced"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="TERB1"/>
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    </dbReference>
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        <property type="entry name" value="TELOMERE REPEATS-BINDING BOUQUET FORMATION PROTEIN 1"/>
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    </dbReference>
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    <dbReference type="SUPFAM" id="SSF46689">
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    </dbReference>
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        <location>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926484.1"/>
        </source>
    </evidence>
    <sequence length="790" mass="90907" checksum="C944CBC2AE0FEDFB" modified="2020-10-07" version="1">MENGKDQKITCDMKTDLNLLLECLKYQMDHPTSQKEALVTIYSVCQQNSAACDYFREIGGLIFVWNLAKSQMHSLVQEAALFTLGGLAENNVFCQQMLCTAELFKDLRTFLTNKDSSMNLKRMSVYVLLVLVSNNNSGQTHMRESGCLSVLLQLFRIIPSESQMNLFDHKIDQYHLWSSVCSNLCVCVNNPQNEENQKACSLVFPYAKDWLQSSMKAEIIRPICSFIGLTVANNSRTQNFFISIGGLAVLDELLLKLIHGSLENNTNLKLAVVVIKTLDACIANNATVGIHLSKYNIVPNLLKLLSYNILDSGERFSIILTLGHCTEVCEENQYTLIKNNGLPLMIQALTEMQDDELNKAATFVLENCKQMTEKLSVKMNQHSFQEDERRILEVDWENKERILEDYWNEAKQILNRIKGLENEQEENIRREILINEDTPQEKFFQAMTQYRQTNSSKQPLENSKICSKMKHPRQNTISKVQNTSKFEKSSSSREQLKSAVWNSTDASLTQNDHNNLYAAEKTTTPNINEAYITNKNSFCDFRKVDAQASEIIFKKPDCVVKSPMRKIQHSELKYPRNPSRGMGHILTVPPKIRELRCSGCRIGRLSLNSRNFSKTLQSCPYLCEQHKIILESEMEYKRKLRRSIVAHENTSAYNSNHNDRRVSCKRSSTTVKELANFQCDAHSKNQTMKQNYSLQDLDLSENHPFEKESTECCSIQNTETLSNRSTTTLKKRRIRKDFTSEEISYLFEGVRKMGHHWNSILWAYPFQKGRTNVDLAKKYCKLQVFFVLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS08</accession>
    <name>A0A6I9YS08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine palmitoyltransferase 1</fullName>
            <ecNumber evidence="5">2.3.1.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Long chain base biosynthesis protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Serine-palmitoyl-CoA transferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SPTLC1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-serine + hexadecanoyl-CoA + H(+) = 3-oxosphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:14761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="EC" id="2.3.1.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:14762"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dodecanoyl-CoA + L-serine + H(+) = 3-oxotetradecasphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35679"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:71008"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:35680"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>octadecanoyl-CoA + L-serine + H(+) = 3-oxoeicosasphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:33683"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:65073"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:33684"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tetradecanoyl-CoA + L-serine + H(+) = 3-oxohexadecasphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:71007"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:35676"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013926405.1">
        <property type="nucleotide sequence ID" value="XM_014070930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS08"/>
    <dbReference type="GeneID" id="106552613"/>
    <dbReference type="KEGG" id="tsr:106552613"/>
    <dbReference type="CTD" id="10558"/>
    <dbReference type="OrthoDB" id="3168162at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000049">
        <property type="entry name" value="serine palmitoyltransferase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF2">
        <property type="entry name" value="SERINE PALMITOYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="20">
        <location>
            <begin position="101"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013926405.1"/>
        </source>
    </evidence>
    <sequence length="475" mass="53393" checksum="64A8C298A93E7FDE" modified="2020-10-07" version="1">MAAEPMGQQWVLVEMVQAFYEAPAYHLILEGILILWIIRLLFSKTYKLQERSDLTPKEKEELIEEWQPEPLVTPVPKDHPALNYNIVKGPPAHNIIVNDKECINFASFNFLGLLDNERLKNAAQASLAKYGVGTCGPRGFYGTFDVHLELEEKLARFMKTEEAILYSYGFATIASAIPAYSKRGDILFVDEAACFAVQKGLQASRSNIKFFKHNDMADLEYLLQEQEIEDQKNPRKARVTRKFIVVEGLFINTGDICPLPELVKLKYKYKVRLFLEESFSFGVLGEHGRGVTEHFGISIDDIDLISANMENSLASIGGFCCGRSFVIDHQRLSGQGYCFSASLPPLLAAAAIEALNIMQENPGIFQVLKQKCKQIHKALQGIPGLRLVGQSFSPALHLQLEESTGSRESDKRLLKEIVEYCMNRGIALTQARYLEKEEKYLPPPSIRVVVTVEQTEKELDKAASLIREAAESVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS45</accession>
    <name>A0A6I9YS45_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Paired box protein Pax-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106552643</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926445.1">
        <property type="nucleotide sequence ID" value="XM_014070970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS45"/>
    <dbReference type="GeneID" id="106552643"/>
    <dbReference type="KEGG" id="tsr:106552643"/>
    <dbReference type="OrthoDB" id="3225452at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022130">
        <property type="entry name" value="Pax2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF19">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12403">
        <property type="entry name" value="Pax2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired-box protein 2 C-terminal" evidence="8">
        <location>
            <begin position="158"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926445.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="28569" checksum="390287B7D8807ED7" modified="2020-10-07" version="1">MAMVEDLQLRIIRTKVQQPFHPTPDGSGTGVTAPGHTIVPSTASPPVSSASNDPVGSYSINGILGIPRSNGEKRKRDEEVTESSVPNGDSQSSVDSLRKHLRADTFTQQQLEALDRVFERPSYPDVFQASEHIKSEQGNEYSLPALTPGLDEVKSSLSTSANPDLGNNVSGPQTYPVVTGRDMASTTLPGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNPYSHPQYTTYNEAWRFSNPALLSSPYYYSATSRGSAPPTAAAAYDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSQ1</accession>
    <name>A0A6I9YSQ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Small serum protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106553369</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-microseminoprotein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927317.1">
        <property type="nucleotide sequence ID" value="XM_014071842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSQ1"/>
    <dbReference type="GeneID" id="106553369"/>
    <dbReference type="KEGG" id="tsr:106553369"/>
    <dbReference type="OrthoDB" id="6076852at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1900">
        <property type="entry name" value="Beta-microseminoprotein (PSP94) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008735">
        <property type="entry name" value="PSP94"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10500">
        <property type="entry name" value="BETA-MICROSEMINOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10500:SF7">
        <property type="entry name" value="BETA-MICROSEMINOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05825">
        <property type="entry name" value="PSP94"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026786112" evidence="5">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013927317.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12386" checksum="C20E261FE4CADD18" modified="2020-10-07" version="1">MKVFFSLILFSLVLATCQGACFLDTFKLEFKDGKPVSSTVCVDVFDQTRHLIGSTWNTANCFRCECDNDGMSCCHRYGGIGLIEGCKTVINPVTCEQEHYRIDDPSQRCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTF1</accession>
    <name>A0A6I9YTF1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Pro-neuregulin-3, membrane-bound isoform-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106553583</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927597.1">
        <property type="nucleotide sequence ID" value="XM_014072122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTF1"/>
    <dbReference type="GeneID" id="106553583"/>
    <dbReference type="KEGG" id="tsr:106553583"/>
    <dbReference type="OrthoDB" id="9939684at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040180">
        <property type="entry name" value="Neuregulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100">
        <property type="entry name" value="HEREGULIN-NEUREGULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100:SF18">
        <property type="entry name" value="PRO-NEUREGULIN-3, MEMBRANE-BOUND ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013927597.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="38514" checksum="314362986606D7FE" modified="2020-10-07" version="1">MSEGVASPPGAAAAAEEGAAAAGGGPEAADGGPEAAGREVRCGDCLVWNRQQTWLCVVPLFIGFVGLGLSLMLLKWIVVGSVQEYLPTELMDSKGIEQDPFFLSKQPTTPPKSAELTASPGTAAAAAASRTPNQISTRLTTMTKAPTRFPATRGPVRAVPPRSTTARHTLAPPTVLASSTAAVAVPFSTTLLVARPAPGAPRSPPVPLWPTAAYTTSSYKTYLQDSAPSWTLSPFQEASTTTTATPETHSSPKYHTTTYSIEQSEHFKPCKDKDLAYCLHDGECFVIETLTGSHKHCRSLQPFSSKRQIQVGGAIWILAVVTVCMFADLYIGNEHLILLAEASGWNNFYIRESGKVTLSHED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTP0</accession>
    <name>A0A6I9YTP0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-binding cassette sub-family A member 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106553442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927416.1">
        <property type="nucleotide sequence ID" value="XM_014071941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTP0"/>
    <dbReference type="GeneID" id="106553442"/>
    <dbReference type="KEGG" id="tsr:106553442"/>
    <dbReference type="OrthoDB" id="6512918at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF98">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE ABCA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013927416.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="18823" checksum="ACD809CEF868914D" modified="2020-10-07" version="1">MTGRETLSMYARLRGIPERYIGSCVENMLRGLLLEPYANKLIRTYSGGNKRKLSAGISLIGGPPVVFLDEPSTGMDPVARRLLWDAVARTRECGKSIISTSHSMEECEALCTRLAIMVNGQFKCLGSPQHLKSKFGSGYTLLAKTHWDEEGHVEDFKAFVERTFPGKPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUI8</accession>
    <name>A0A6I9YUI8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 585A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106553357</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927305.1">
        <property type="nucleotide sequence ID" value="XM_014071830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUI8"/>
    <dbReference type="GeneID" id="106553357"/>
    <dbReference type="KEGG" id="tsr:106553357"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Zinc finger protein 200"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
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        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Zinc finger protein 35"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Zinc finger protein 45"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger protein 70"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="24"/>
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        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="22"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="24"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="14"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
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    <sequence length="1165" mass="132228" checksum="2A1BD402F61C0291" modified="2020-10-07" version="1">MPVNFKEVAMYFPEDQDLMSDINQRSLYESGMQENAGGGQIHEKKEDVLGQNYNQNQQTGTSPGAAGKVVSLFFEEGEPTVKHLNPERQRQYHLAKRRDSSAAYTQVPQPPGQGASGKTRRRSKIPRTCNECGKTFAQASNLLAHKRIHTGEKPYKCVHCGKSFTERSNRNRHQRTHSGQKPYNCLDCGKSFSFRSDLIRHGITHTGERPYKCSDCGKSFSHASTLIRHENIHTGEKPYSCADCGKCFTQSSSLLAHKRLHTGETPFICPICGESFNWKSHLVTHKRTHTGERPYKCGKCEKSFIEKSKLNRHQRTHAEKELYGCGECGRIFSLRSNLVRHETAHRAAKSHNCLSCGKSFNQAAKLMRHERVHTGEKPYSCSGCGKSFSQTSELLRHERIHTGEKPYKCSACGKCFNRKSHLSTHEATHLADLPRGGLQIRRVYDSSSLFIAGLSPHQQTHAEGQAYSGSESEKDYSHSSIFIAGLNPSRPTHVGDQLHEWPNSETSYGHCSLLITGVNPQEGTQAEEPLFQCLDRETSYTRSPLFKSIMNPHQVALSGGQSISCVDREKNCSDPSLFQKEDKLFKCSECGKSFNWQSHFTRHKRIHTGEKPYCCTDCGKSFNRRSHLVRHSRTHNGLSLYSQTVEESSVQDLVFLNPRESMQERTVINTQSVGASSFLPCTRLPPGNLTILPAEPTQELKRLLTWKKIEYVELILNDTLYKKVKGKGYLQIQKKMEVPGNQWRGQLDNQVWQAVMQDSLCEEGKSSLNENIIQEGIQHIFDLPNKPRISVVEKPHGATGGEDSMDCKSQLMVHESIHVREKRNICSTCGKLFCYSPRRFEDKMTLTEGNTYQCSECDKNFCKDATLAIHRRTNTEEKQYVCLDCGKTFNRNSHLVIHQRTHTGEKPYECPDCGKSFSRKSNLLIHKRIHTGEKPYSCQDCGKSFSRNSNLVTHERTHTGEKPYECQYCGKCFSQNSHLVIHQKIHTGEKPYECLECGKSFSWNSHLVIHQRTHTGEKPYGCPVCGKTFSQKSDLMMHQRTHTGEKPYKCPLCGKTFNRNSHLLIHQNTHTGVKPYECLECGKTFSQSSHLLIHQRSHTGEKPYECQECGKGFSTRSNLINHLGLHTGEKPFKCLECGRCFTQYSCLAAHKKIHMRQKAMTVEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUX4</accession>
    <name>A0A6I9YUX4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PAK2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013928403.1">
        <property type="nucleotide sequence ID" value="XM_014072928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUX4"/>
    <dbReference type="GeneID" id="106554254"/>
    <dbReference type="KEGG" id="tsr:106554254"/>
    <dbReference type="CTD" id="5062"/>
    <dbReference type="OrthoDB" id="1022360at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06655">
        <property type="entry name" value="STKc_PAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035064">
        <property type="entry name" value="STK_PAK2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF21">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="246"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="162"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013928403.1"/>
        </source>
    </evidence>
    <sequence length="521" mass="57745" checksum="27EFCD1B54971421" modified="2020-10-07" version="1">MSDNGELEDKPPAPPVRMSSTIFSSGGKDPLSANHSLKPLPSVPEERKPRNKIISIFSSTEKGSRKKEKERPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSKDTAKQKYLSFSATDKDGFLSGAIALNAKRSEPSTAAEEEEEDEVAPPVIAPRPDHTKSVYTRSVIDPIPAPSGDLSDNAKLADKQKKKNKMTDEEITERLRTIVSIGDPKKKYTRYEKIGQGASGTVFIAVDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQSPEKLTPIFRDFLNSCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUZ8</accession>
    <name>A0A6I9YUZ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Caveolin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106554303</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <location evidence="10">Caveola</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the caveolin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928428.1">
        <property type="nucleotide sequence ID" value="XM_014072953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUZ8"/>
    <dbReference type="GeneID" id="106554303"/>
    <dbReference type="KEGG" id="tsr:106554303"/>
    <dbReference type="OrthoDB" id="5917823at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070836">
        <property type="term" value="P:caveola assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001612">
        <property type="entry name" value="Caveolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018361">
        <property type="entry name" value="Caveolin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844:SF16">
        <property type="entry name" value="CAVEOLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01146">
        <property type="entry name" value="Caveolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01210">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928428.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="24313" checksum="C494FEA596DE0510" modified="2020-10-07" version="1">MEDLSLWYSLFSTKNAKTNGAVPGSNHWFLSQISLAKRKEDFLKQYKCRIKRIRRYEKQFAKRYLKHLEGLKETLIYQAITFRTIEIWVTASILVDFEDVIAEPEGTHSFDGVWKASNTTFTVSKYWCYRILSGVLGLPLALLWGFLFACVSFCHIWGAMPCIKSYFIEVQCCGRCYALCIRTFCDPLFEAVGKVCSDVRVALRKEHAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YW43</accession>
    <name>A0A6I9YW43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoplasmic reticulum membrane protein complex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ER membrane protein complex subunit 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EMC7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928512.1">
        <property type="nucleotide sequence ID" value="XM_014073037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YW43"/>
    <dbReference type="GeneID" id="106554388"/>
    <dbReference type="KEGG" id="tsr:106554388"/>
    <dbReference type="CTD" id="56851"/>
    <dbReference type="OrthoDB" id="27095at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019008">
        <property type="entry name" value="Beta_sandwich_EMC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039163">
        <property type="entry name" value="EMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09430">
        <property type="entry name" value="EMC7_beta-sandw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026981681" description="Endoplasmic reticulum membrane protein complex subunit 7" evidence="12">
        <location>
            <begin position="28"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="13">
        <location>
            <begin position="57"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013928512.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="26603" checksum="F6046FA934C899C6" modified="2020-10-07" version="1">MAAGPFSLFGTVFLLAATFQFPSCSWASETPGGGGGDALGAGERFKIEGRAVVPGVKPQDWIAGARVLVDGEEHVGFLKTDGSFVVHDIPSGSYVVEVISPAYKFEPVRVDITSRGKMRARYVNYIKTSEVVRLPYPLQMKSSGPPSYFIKRESWGWTDFLMNPMVMMMILPLLIFVLLPKVVNTSDPDMRREMEQSMNMLNSNHELPDVSEFMTRLFSSKSSSKSTGSSSKIGKSGAGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWC2</accession>
    <name>A0A6I9YWC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Elongation factor Tu GTP-binding domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928948.1">
        <property type="nucleotide sequence ID" value="XM_014073473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWC2"/>
    <dbReference type="GeneID" id="106554741"/>
    <dbReference type="KEGG" id="tsr:106554741"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000732">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000002">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="36"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928948.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="47523" checksum="4F18636F9DF80CE9" modified="2020-10-07" version="1">MRTGAPFEKRRGDTVKHQTMVPPRSNKTSGLQQNTACIRNICILAHVDHGKTTLADCLISSNGIISRRLAGKLRYLDSREDEQIRGITMKSSAISLLFVKENQEHLINLIDSPGHVDFSSEVSTAVRLCDGCIIVVDAVEGVCPQTHAVLRQAWLENIRPVLVINKIDRLILELKFTPQEAYSHLKNILEQVNAITATLFTSQILEKRAEKDTDAQLDFDPTNVDQVYDWSAGLEFTDDSHLYFSPDQGNVVFASAIDGWGFGIEHFAKLYSQKMGIKPSVLLKTLWGDYYLNMKAKRIMKVDQLKGKKPLFVQLVLENIWSLYEAVTKRDKEKIEKIISSLGLQIAARELRHTDPKVQLNAICSQWLPISDAVLSMVCDKLPSPLDITAERVEKLLCIGAKTFDSLPQETQDLKEGEYTLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWG7</accession>
    <name>A0A6I9YWG7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">EF-hand calcium-binding domain-containing protein 4B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928993.1">
        <property type="nucleotide sequence ID" value="XM_014073518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWG7"/>
    <dbReference type="GeneID" id="106554785"/>
    <dbReference type="KEGG" id="tsr:106554785"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928993.1"/>
        </source>
    </evidence>
    <sequence length="74" mass="8811" checksum="F05390C81FDC45C6" modified="2020-10-07" version="1">MSAYDDEIQHLYEEMEQQIKTEKEKVVLEGAERFQSRQQELEQKLVEKEKEFEQLTQRQKIVGGNYCVVIAADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWR3</accession>
    <name>A0A6I9YWR3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Purkinje cell protein 4-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PCP4L1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCP4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928045.1">
        <property type="nucleotide sequence ID" value="XM_014072570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWR3"/>
    <dbReference type="GeneID" id="106553975"/>
    <dbReference type="KEGG" id="tsr:106553975"/>
    <dbReference type="CTD" id="654790"/>
    <dbReference type="OrthoDB" id="9944346at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052142">
        <property type="entry name" value="Calmodulin_Regulator_PCP4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15359">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15359:SF5">
        <property type="entry name" value="PURKINJE CELL PROTEIN 4-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928045.1"/>
        </source>
    </evidence>
    <sequence length="69" mass="7840" checksum="0C15975551138515" modified="2020-10-07" version="1">SNEGLVCKRHWKTATIVFSPADKAENPKKTEPEEEEVDIDLNAPETEKAALAIQGKFRRFQKRKQDPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY59</accession>
    <name>A0A6I9YY59_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ankyrin repeat and SAM domain-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ANKS6</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928871.1">
        <property type="nucleotide sequence ID" value="XM_014073396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY59"/>
    <dbReference type="GeneID" id="106554674"/>
    <dbReference type="KEGG" id="tsr:106554674"/>
    <dbReference type="CTD" id="203286"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051631">
        <property type="entry name" value="Ankyrin-KH/SAM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23206:SF5">
        <property type="entry name" value="ANKYRIN REPEAT AND KH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23206">
        <property type="entry name" value="MASK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="135"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="315"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="349"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="577"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="650"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013928871.1"/>
        </source>
    </evidence>
    <sequence length="662" mass="71722" checksum="8732B7F70A1759A3" modified="2020-10-07" version="1">MGECGVPAGVQLLLRACDQGDCEGARRLLAGPEEKAAAEAGSGSPSGGGVGAAADIPPGSVPVDCTDEAGNTGLQFAAAGGHEELVGFLLRRGASVQSRNHYGWSPLMQAARFGHLNVARILLENGADLNAQNKLGASVLTIASRGGHISVMKLLLESGAYVDNYDHLSISLDGSKEEMPAVTPLMTAVQHGHEAAVHLLLDWGADCNYSVKTMGWTPLMLAAVSGRASLAQQLMNKGANPDHLNILHKTPYEISVDFKHEDMMDYLAPLTTVRPQTDKQKKQPDIFHALKMGNLQLVKEIIDEDPAQSNVINDDGASPLMIAAVTGQMPLIQLLVSRNVDIDKQDNVHGWTALMQATYHGNKEVVKYLLNQGADVNLRAKNGYTAFDLIMLLNDPDAELVRLLASACMQEKSKQSSKVNAPFSRNKQPIGIPLLPDDKGGLKSWWNRMSNRFRKLKMTQTLNHGFSMNQSIPFPEESKSPFDCTMKAALQNDNTVNHDATTTWATKSKSNVIHPGKTGKDDDFLTTVLRSGAPFTRLPNDKLKAVIPPFLPPSSFELWNSDRTQNKEQTRLGFPHRSTSIDNSTDKTSLSRGSRPIKLSTFARRPPTPSNSSNFNHSPHSSGGSSSVGGVSRHGGELHNKSGRIYSSKCKVDSDPEQKHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYS0</accession>
    <name>A0A6I9YYS0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rho GTPase-activating protein 20-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106555510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929853.1">
        <property type="nucleotide sequence ID" value="XM_014074378.1"/>
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    <dbReference type="GeneID" id="106555510"/>
    <dbReference type="KEGG" id="tsr:106555510"/>
    <dbReference type="OrthoDB" id="9994905at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179:SF28">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE ACTIVATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013929853.1"/>
        </source>
    </evidence>
    <sequence length="182" mass="20442" checksum="5AE46343BCAE79DB" modified="2020-10-07" version="1">MQDCIFLKGSEKDYQLWVNSGKEDAPYPLIGHEYPFGIKMSHIRDTLPLTQGSKDGDCPLDLQGAFLIEQLPREHQCQFILKPSRLTTGHQLNELSQKPFKRKRSIINWAFWRETGAPVDHLVPSPTSSKAGKLFGLSLSSICENDNLPKPVLVSLFDILSRMMGTLWLKMCTSSPGTHLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZD7</accession>
    <name>A0A6I9YZD7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger protein 608</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ZNF608</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
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            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
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    <dbReference type="OrthoDB" id="5863628at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ZN608/ZN609"/>
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    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
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    <dbReference type="PANTHER" id="PTHR21564">
        <property type="entry name" value="BRAKELESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564:SF4">
        <property type="entry name" value="ZINC FINGER PROTEIN 608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="80"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="401"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="645"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="753"/>
            <end position="807"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="864"/>
            <end position="886"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="904"/>
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            <begin position="987"/>
            <end position="1046"/>
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            <begin position="1097"/>
            <end position="1170"/>
        </location>
    </feature>
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            <begin position="1236"/>
            <end position="1285"/>
        </location>
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            <begin position="1397"/>
            <end position="1439"/>
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            <begin position="49"/>
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        </location>
    </feature>
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            <begin position="80"/>
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            <begin position="151"/>
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        </location>
    </feature>
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            <begin position="190"/>
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        </location>
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            <begin position="424"/>
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            <begin position="435"/>
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        </location>
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            <begin position="512"/>
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            <begin position="1252"/>
            <end position="1265"/>
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        <location>
            <begin position="1266"/>
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    </feature>
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        <location>
            <begin position="1412"/>
            <end position="1428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS00028"/>
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            <dbReference type="Proteomes" id="UP000504617"/>
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    <sequence length="1494" mass="163017" checksum="7CCA3D2B3DA5735F" modified="2020-10-07" version="1">MSLNTSTAGKCVDPNTVDTYDSGDDWEIGVGNLIIDLDADLEKDRQKFEMNNSTNSKDCGSLASSGVNPTSALADGLKFSSVQPPASQGNSSHKETSKSKVKRSKTSKDASKSLPSAALYGIPEISSAGKRQEVQGRPSEAGGMNSALGQSMNSSPNGNNSTTSINNNTLANCGKTKEEKTGKTQASRGSKRDKDGGKSRKDRQLDLQQGPSNNSSQTAPGHLYSFGAKGGCAGSSIPFNCTSSSTAGDVNKSAPDSGLMGNSILVKKEEEEEESHRRIKKLKTEKVDPLFTVPAPPPPISSNLTTQILPSYFSPSSSNIAAPVEQLLVRTRSVGVNTCDVGIVTEPECLGPCEPGTSVNLEGIVWHETEEGVLVVNVTWRNKTYVGTLLDCTKHDWAPPRFCESPTSDLEMRGGRGRGKRARSAATTTTPGTDITFTESRGLQNKNRGGANGKGRRGSLNSSGRRTPPNCAVEDVKVSPLSNNKRKNKPPMELDLNSSSEDNKSGKRVRTNSRSTPTTPQGKPETAFLDQGCSSPVLIDCPHPNCNKKYKHINGLRYHQAHAHLDPENKLEFEPDSEDKISDCEEALGNVALECSDPSTSLSHFDQLKGPLSPCSLSTPGTPKGKREQAQNGRNSGKKRGLNSELNSLPVISNMTVTLENCSVAENSLASEMPKLEAEGLIDKKNLGEKDKGKKANNCKVDKNLSKLKTARPIAPAPAPTPPQLIAIPTAAFTTTTTGSISGLPSLTTTVVQATPKSPPLKPIQPKPTIMGEPNTVSPALTLLKDKKKKEKRKLKDKESKETGNPKVDLKLGKLEDTKGSGKEISGHFLKEHLSKNEVLANGLSESQESRMASIKAEADKVYTFTDNAPSPSIGSSSRMECSPLVNGQSSVAPLHVLTQNGAEGAAAKTNSPAYSDISDAADDGGSDSRSEGMRSKASSPSDIISNKDSVGKGHSSTPVQLPQVKEAHSPYYHGYDPYYSPSYMHSGQVSTPAAGNSSNNPQGMKIKKEAEEEAEKKDKAEPPESKKSEAGPTNLQPQHQSVITQRHPALAQSLYYGQYAYGLYMDQKSLMASNPAYRQQYEKYYEDQRLAEQKVTQTGRECERKNELPLKDLGKDDKLKNIPSATISKAPSASETSKNNTKIGSSVPNKTDETSKSQMLSNHQQQIQSDSFKAKQMENHQLIKEAVEMKSVMDSMKQTGVDPTTRFKQDPDTRTWHHYVYQPKYLDQQKVEELDREKKLKEDSPRKTPNKEASFSSLPVSLNSIKEEPKEMKRSDSQPLEENKIKNDDQKTAVNWKESRGTRVAVSSPMTQHQSYIQYLHAYPYPQMYDPNHPAYRAVSPVLMHSYPGAYLSPGFHYPVYGKMSGREEVEKVNTSPSISVKSTTESKALDLLQQHANQYRSKSPVPVEKSTAEREREAERERDRHSPFSQRHLHTHHHTHVGMGYPLIPGQYDPFQECQFIVDVTEDDSIIDDDFNNNLDDNIVHDDVRIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0J5</accession>
    <name>A0A6I9Z0J5_9SAUR</name>
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        <submittedName>
            <fullName evidence="9">ATP-binding cassette sub-family D member 2-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="9" type="primary">LOC106555728</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
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        <property type="nucleotide sequence ID" value="XM_014074622.1"/>
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    <dbReference type="OrthoDB" id="422637at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="ABC1_TM_dom"/>
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        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="138"/>
            <end position="412"/>
        </location>
    </feature>
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        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
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        <location>
            <begin position="356"/>
            <end position="383"/>
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        <location>
            <begin position="101"/>
            <end position="117"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF06472"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="532" mass="59214" checksum="977B0ADBB1AE8C85" modified="2020-10-07" version="1">MNGGGGAWRRSPRSGIFFQPGRAAAEEEEEIPLYPCLSAGSPDGTMSPALQSLLPGRAGRWSRTSAAKRAAALVAAAYGLKILWPLLCKRLRRQQRSGQDAAKREEKEAMGSLHRPDPAASKASPAANAEFLRQLLALRKIFFPRLVSPESGWLCLHSVALVSRTFLSIYVAGLDGQIVKSIVEKRPRSFACKLLKWLLVAIPATFVNSAIRYLECKLALACRTRLVDHAYLTYFDHQTYYKVTNMDGRLANPDQSLTEDIMMFSQSVAHLYSNLTKPILDVGLTSYTLIRTAQARGASPVGPTLLAGLVVYTTAKVLRACSPKFGKLVAEEAHRKGYLRYLHSRIIANGEEIAFYRGHKVEMNQLQKSYKALEEQMNVILSKRLWYIMLEQFLMKYVWSSSGLIMVAVPIITATAFADGDLDNGQKQAMVSERTETFTTSRNLLVSGADAIERIMSSYKEVAELAGYTARVYNMFSVFDEVKRGIYKMSDLTPERESSNKKRSKAKQCINGPLEIKGKAQWDFKSKAVPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0R3</accession>
    <name>A0A6I9Z0R3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Argininosuccinate lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ASL</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930593.1">
        <property type="nucleotide sequence ID" value="XM_014075118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0R3"/>
    <dbReference type="GeneID" id="106556142"/>
    <dbReference type="KEGG" id="tsr:106556142"/>
    <dbReference type="CTD" id="435"/>
    <dbReference type="OrthoDB" id="2561043at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000002">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000002">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000015">
        <property type="entry name" value="argininosuccinate lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="3">
        <location>
            <begin position="368"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930593.1"/>
        </source>
    </evidence>
    <sequence length="464" mass="51823" checksum="A2EE9DE05674D61C" modified="2020-10-07" version="1">MAAESDKLYGGRIVGSIDPIMEQFNASIEIDKRLSEFDICGSMAYAKALEKAGILSKVEMEKIISGLEKISDECSKGSLIISRTDEDIHTTVERRLKELIGDVAGKLHTGRSRNDQIVTDLRLFMKSSMTMLSVHLQQLIRTLMERASTEMEVIFPGYTHLQKAQPIRWSHFLLSFAVALARDHERLFEAKKRINVLPLGSGALAGNPFGLDRELLRRELDFATISINSMDAVSDRDFAVEFLYIASMIMVHLSRLSEDLILYATKEFGFITLSDAYCTGSSLMPQKRNPDSLELIRSKAGRVIGRATGLLVILKGLPSTYNKDLQEDKEAVIDVMDTLSTVLKVTTGVISTLQINRDNMERALTPEMLSTDLAYYLVRKGVPFRQAHTVVGKAVRLAESKGVTLNHLSMDDLKGIHPMMGSDISQIFNHQHSVEQYTSPGGTARSCVSAQIEYLKEMLKRQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z277</accession>
    <name>A0A6I9Z277_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein orai-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ORAI2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orai family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930334.1">
        <property type="nucleotide sequence ID" value="XM_014074859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z277"/>
    <dbReference type="GeneID" id="106555919"/>
    <dbReference type="KEGG" id="tsr:106555919"/>
    <dbReference type="CTD" id="80228"/>
    <dbReference type="OrthoDB" id="61124at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002115">
        <property type="term" value="P:store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.140:FF:000001">
        <property type="entry name" value="Calcium release-activated calcium modulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.140">
        <property type="entry name" value="Calcium release-activated calcium channel protein Orai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012446">
        <property type="entry name" value="CRAC_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038350">
        <property type="entry name" value="Orai_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501">
        <property type="entry name" value="CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501:SF5">
        <property type="entry name" value="PROTEIN ORAI-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07856">
        <property type="entry name" value="Orai-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930334.1"/>
        </source>
    </evidence>
    <sequence length="266" mass="30060" checksum="B75700DB00BA19AC" modified="2020-10-07" version="1">MDFNSLTFIMSSELNVPVDPPSSTCCAEPTGKGMDYRDWVRRSYLELVTSNHHSVQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLEMQYQYPPMLLIAFSACTTILVAVHLFALLISTCILPNVEAVSNIHNLNSISESPHERMHLYIELAWGFSTVLGILLFLAEVVLLCWIKFLPVDSALRNDSTVIQKTSQHTGWHAALISTIIMVPVGIIFVVFTIHFYRSLVLHKTERHNREIEELHKLKVQLDGHDRGLQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2K8</accession>
    <name>A0A6I9Z2K8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Discoidin, CUB and LCCL domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Endothelial and smooth muscle cell-derived neuropilin-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DCBLD2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930464.1">
        <property type="nucleotide sequence ID" value="XM_014074989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2K8"/>
    <dbReference type="GeneID" id="106556034"/>
    <dbReference type="KEGG" id="tsr:106556034"/>
    <dbReference type="CTD" id="131566"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.130.20:FF:000002">
        <property type="entry name" value="Discoidin, CUB and LCCL domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000035">
        <property type="entry name" value="Discoidin, CUB and LCCL domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF3">
        <property type="entry name" value="DISCOIDIN, CUB AND LCCL DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027123197" description="Discoidin, CUB and LCCL domain-containing protein 2" evidence="14">
        <location>
            <begin position="36"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="41"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="17">
        <location>
            <begin position="158"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="16">
        <location>
            <begin position="261"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013930464.1"/>
        </source>
    </evidence>
    <sequence length="748" mass="83040" checksum="C84988F9EF336635" modified="2020-10-07" version="1">MVSGRERLLHLVPPGTRPAALPLLLLLLLRRDAVAQKGDGCGYTVLGPESGTFTSINYPHAYPNSTVCEWEIRVKPDQRVQLKFGDFDIDDSDSCHFNYVRIYNGIGPTRTEIGKYCGLGLQVDKLNESKSNEVTVQFMSDVHFYGHGFLASYSTTDKPDLITCLDTASHFSEPEFSKYCPAGCVLPFAEIYGTIPHGYRDSSSLCMAGVHAGVVSNILGGQINVVISKGIPYYESSLANNVTSKVGPLSSGLFTFKTSGCYGTLGMESGVIADSQINASSFLEWPEQLGQPSTWKPERARLKRPGHSWAAFTNDEYQWLQIDLNKEQRITGIITTGSTLAEYHYYVSAYRILYSNEGQKWTVYREPGVERDKVFQGNTGYYQEVRNNFIPPIIARFVRINPLKWHQKLAMKVELLGCQFNIVRAPRPSLPPPPRSNNDFSLQIDKTTFTPEVKNTMLTPSVTKVRSPRATLPPPPRSNKDFPLQTSKTTFAPKIKNTTVTPSVTKDVALIAVLVPVLVMVFTTLILISVCSWHWRNRKRKSEGTYDIPYWDRAGWWKGMKQFFPAKSVDHEESSVHYNSSEINCLRSRDATTVLQSQSAEYAQPLVGGIVGTLNQKSAFKREEVKEASYADKDSCNTSEQEIYHAYAEPLPVTGPEYAIPIVMGMSGHPAPPPGIASVSTFKASGNQAPPLVGTYNKLLSRADSSLSATHVLYDTPKGMPGLSLPNELVYQIPQNMPHSTDNKEDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3T5</accession>
    <name>A0A6I9Z3T5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras-related protein Rab-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106556418</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Lipid-anchor</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930889.1">
        <property type="nucleotide sequence ID" value="XM_014075414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3T5"/>
    <dbReference type="GeneID" id="106556418"/>
    <dbReference type="KEGG" id="tsr:106556418"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01865">
        <property type="entry name" value="Rab3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000206">
        <property type="entry name" value="Ras-related protein Rab-3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037872">
        <property type="entry name" value="Rab3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013930889.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24947" checksum="844DC4A42DCC2437" modified="2020-10-07" version="1">MASATDPRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERGRQLAEHLGFEFFEASAKDNINVKQTFERLVDIICEKMSESLDAADPAVTGAKPGPQLTDQQAPPHQDCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z402</accession>
    <name>A0A6I9Z402_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dystonin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106556873</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931382.1">
        <property type="nucleotide sequence ID" value="XM_014075907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z402"/>
    <dbReference type="GeneID" id="106556873"/>
    <dbReference type="KEGG" id="tsr:106556873"/>
    <dbReference type="OrthoDB" id="10016565at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="153"/>
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            <end position="189"/>
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            <begin position="194"/>
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            <begin position="331"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013931382.1"/>
        </source>
    </evidence>
    <sequence length="396" mass="45485" checksum="713F87B77915611C" modified="2020-10-07" version="1">MEEMTRKQPDVDKVTKTHKRKAAETSPVQSHIPVLDKGRGGRKRSPIPSMYPSGAQTQIETKNPRINLLLSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAYKPLTDADKIEDEVTRQVAKCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRVHHHGSKMLRSESNSSITTQPTLAKGRTNMELREKFMLADGPSQSMAAFRSRGRRSRPSSRGASPNRSMSVSNQAAQGTPVSALAASTPKTRNYGKPWLINSKTSSPCKPSECYEYQVSSTEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4D5</accession>
    <name>A0A6I9Z4D5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">StAR-related lipid transfer protein 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">START domain-containing protein 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106557281</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Homodimer. Interacts with TAX1BP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931963.1">
        <property type="nucleotide sequence ID" value="XM_014076488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4D5"/>
    <dbReference type="GeneID" id="106557281"/>
    <dbReference type="KEGG" id="tsr:106557281"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04375">
        <property type="entry name" value="RhoGAP_DLC1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="rho GTPase-activating protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000009">
        <property type="entry name" value="StAR related lipid transfer domain containing 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2070:FF:000001">
        <property type="entry name" value="StAR-related lipid transfer domain-containing 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2070">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
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        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12659">
        <property type="entry name" value="RHO-TYPE GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12659:SF6">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="15">
        <location>
            <begin position="670"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="16">
        <location>
            <begin position="905"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="545"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013931963.1"/>
        </source>
    </evidence>
    <sequence length="1119" mass="126987" checksum="906DC52C6BE0177A" modified="2020-10-07" version="1">MFRQMASTSGSGCLCVNAMSTEGQEMYLLFDQTARRSPYRKSRILARQQLLTKIQQEREAKESCAWLRAAGFPQYAQLYEDYQFPIDIAAVKKDHDFLDKDLVETLCRRLNTLNKCASMKLDLNFQRKKSEDSDEEDLCAISNKWTFQRASQRWSRVEDSDALINQSGKHDFQGDIKMKITTSNESVLTDLSEPEVSSIHSESSEGSDHRSQSGITSLLRETNCSRQYCVKNTSVQDPSSIGGPSSQISEVSPNFRNEKASRTRAKTFLKCMETLKPKGQHGKLKGSGLTSLSVIGTPVLQYEPRILKEMQCMEVENGDFQNKTVALQDPVRQGFPFSANCSSENSQLEISNSTISTPSLKERKHHEANKRGGMYLEDVDVLAGATLRQMIDQNCKNEFHSQENLIFHIPKDHKPGTFPKALSIESLSPTDNALNWKRDSVSLTNKISKEHKPLVSCSKESRVSIYDNVPSSHLCASTGDLLDLERENLFPHLDDILQHVNGLQEYIDDWSKNVLPEQKKNALSIGELESLSCQSPSKISLEFEQNSMSDGHTTPSDIDRDGASLNESETPGVRERRDSGVGASLTRPNRRLRWHSFQISHQLSHALGSLQLSNQSAAQLNLLQKFSLLRLTAIMEKYSMSNKHGWTWSVPKFMKWMKVPDYKDKNIFGVPLIVHVQRTGQPLPQSIQQALRYLRNNCLDQVGLFRKSGVKSRIQALRQMNESSPENVSYEDQSAYDVADMVKQFFRDLPEPLLTSKLGETFLHIYQYVPKEQQLRAVQAAIMLMSDENREVLQTLLCFLNDVTSVEENQMTSMNIALCLAPSLFHLNIIKKESSPRVMQKKCTTGKPDQKDLSENLAATQGLAHMITECDRLFEIPHEMFCQSRNSYIEAEVRSPTLEDLGKQMDEENGNYQTYIENLIQNLCKDAKDKFKGWVTCSSMENTDLCYKKVGDGNPLRLWKASVEVEAPPSVVLNRVLRERHLWDEDFLQWKIVETLDKQTEIYQHVLNSMAPHPTRDFLVLRAWRTDLPKGMCVLVAISVEHDETPLMGDVRAVVLDSQYLIEPCGSGKSRLTHICRVDLKGHSLDWYNKGFGHRCLAEVANIRNSFQPLIAEGPETKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z647</accession>
    <name>A0A6I9Z647_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein LOC106556814</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106556814</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931300.1">
        <property type="nucleotide sequence ID" value="XM_014075825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z647"/>
    <dbReference type="GeneID" id="106556814"/>
    <dbReference type="KEGG" id="tsr:106556814"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000005">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SCAN box" evidence="5">
        <location>
            <begin position="60"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="6">
        <location>
            <begin position="152"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013931300.1"/>
        </source>
    </evidence>
    <sequence length="371" mass="42043" checksum="8E680632308EC58F" modified="2020-10-07" version="1">MALAALKTREKMEGQWQAHADVGKGPPVTQPGSCGKNGASLGQKSQEAAARSSEVQCCDFRKVEFQEGKSPRDVCSQLHLLCRQWLQPERHTKAQMLDLVLLEQFLAVLPPEMEQWVRECGAETSSQAVALAEGFLLTQEEEKMEDYLQGVVSFEEVAVYFSKEEWSQLDPAQKALHGEVMLENSRNLFSLEQYLRCYITYQQTNWAELLPFAEVAYNNAIHSSTSHTPFKVVNWVEFVLIPTFPQVPPSLFSLTEWFNTLHGVWGIVKWALLKAAKTQKLHMVMKSLPQKPFHVGKKMYISTKYLKLKLPCRKLGTKFLGPFPVVRVINPVTMELKLPHLLLKVHPVFHSSLLKPVVTSRSRPSTLTVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6C5</accession>
    <name>A0A6I9Z6C5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Germ cell-less protein-like 1 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GMCL1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931385.1">
        <property type="nucleotide sequence ID" value="XM_014075910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6C5"/>
    <dbReference type="GeneID" id="106556875"/>
    <dbReference type="CTD" id="64395"/>
    <dbReference type="OrthoDB" id="6359943at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007281">
        <property type="term" value="P:germ cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18495">
        <property type="entry name" value="BACK_GCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043380">
        <property type="entry name" value="Gcl-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23231:SF17">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23231">
        <property type="entry name" value="GERM CELL-LESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BACK" evidence="2">
        <location>
            <begin position="79"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931385.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="44708" checksum="31B3F5452D42847E" modified="2020-10-07" version="1">MFSGSWRESGMDTIELEIPDQNIDIEALQVAFGSLYRDEVLINPSRVIALLAAASMLQLDGLIQQCGETMKETINAKTVCGYYNSAGTYGLDSLKKKCLEWLLNNLMTHQSVELLKELSINLMKQLISSSNLFVMQVEMDIYTALKKWMFLQLLPSWNGSLKQLLSEADAWFCKRRKDFEDGISFLETEQGYAFIPVFKYLRLQYIISDLASARIIERDSLIPADWLFSVYKQQWFAMLRAEQDNDFGPQEINKEELEANSMRCGRKLAKDGDYCWRWTGFNFGFDLLVTYTNRYIVFKRNTLNQPCSGSVSLQPRRNIAFRLRMASFHSSGKLICSRTAGYQILSLEKDQEQVVMNLDSRLLVFPLYICCNFLYTSPEKRRENDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X177</accession>
    <name>A0A6I9X177_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Sodium channel and clathrin linker 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6" type="primary">SCLT1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907572.1">
        <property type="nucleotide sequence ID" value="XM_014052097.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907573.1">
        <property type="nucleotide sequence ID" value="XM_014052098.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907574.1">
        <property type="nucleotide sequence ID" value="XM_014052099.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907575.1">
        <property type="nucleotide sequence ID" value="XM_014052100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X177"/>
    <dbReference type="GeneID" id="106537835"/>
    <dbReference type="KEGG" id="tsr:106537835"/>
    <dbReference type="CTD" id="132320"/>
    <dbReference type="OrthoDB" id="551053at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045162">
        <property type="term" value="P:clustering of voltage-gated sodium channels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038911">
        <property type="entry name" value="SCLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35970">
        <property type="entry name" value="SODIUM CHANNEL AND CLATHRIN LINKER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35970:SF1">
        <property type="entry name" value="SODIUM CHANNEL AND CLATHRIN LINKER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4 5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3 4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="575"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013907574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013907575.1"/>
        </source>
    </evidence>
    <sequence length="636" mass="75194" checksum="A939000359BA5516" modified="2020-10-07" version="1">MSTDIKNNGFEDDSITEHIMIDQSSLSPIVAEYDKHLGEMNEQLKYCQMQMKEMRLKLEQVIKENERLHEELKEAIEKQLEALPSGSVSRDATFMDENLVRNLQEQLQLANKEKEHVLELWQMVSRELERLQQIYQEHMTEAQIHIVERQKQNINHVFLKTVTEQNVELEQLRKQQRQSKLDLRTTAAKADEMMGLIEDLRSQVNRKEEEVLTAHEKEEASDKHLKELQSSIKQLETRLCVATKDSKQLRSERTNLEKKIQELQTKCVNIEEEKYDAVAKVRDSMQLIEEANLQKSQAMLSEKQKEEEIETMKQAISQLLQDAALRTRKQVENERKRYNIQISRVTEELTALQMECGEKQSQLERAIREKQAVEEELEKVYREGRGNEADYRKLEELYQRCLNAERIKDDLHLSLQTAQNKIKQLELKCEEEKCRCQENMCKLQSILHSEREKCGAISEERLKLQQGNEQLQKEMENLKKLTMEAQHSAKLKISTMENEHLLKEHGFEIQLKEMEDVNQNSVTELRRLLMAQQKATNRWKEETKKITEITESRINCLKTELSRQKLHTQELFYQLERANEKVIESEKLMREHQEKVNRLQNRLNQAEERATTATQQLSAITLQKKKAAYLIEQKEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1D1</accession>
    <name>A0A6I9X1D1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division cycle-associated protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CDCA4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908146.1">
        <property type="nucleotide sequence ID" value="XM_014052671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1D1"/>
    <dbReference type="GeneID" id="106538229"/>
    <dbReference type="KEGG" id="tsr:106538229"/>
    <dbReference type="CTD" id="55038"/>
    <dbReference type="OrthoDB" id="8735401at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052262">
        <property type="entry name" value="E2F-SERTA_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009263">
        <property type="entry name" value="SERTA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16277">
        <property type="entry name" value="CELL DIVISION CYCLE ASSOCIATED PROTEIN 4/SERTA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16277:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06031">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51053">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERTA" evidence="1">
        <location>
            <begin position="45"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013908146.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="29392" checksum="809AB4B910ACB23D" modified="2020-10-07" version="1">MGEEEGLHQAPLLDTMFVRGLKRKRIEGEDDIEGSFTGFKAIPSYNLQRQTLLDMSLVKLQLCHMLVEPNLYRSVLIANTVRQIQEEMTQDGSWQMINPQSTGPASLDRLVSTDILCRSSKEQTEEKLAQEYSGFSKNFEDTQSQENVDILRPAVLQASRSLQGNMWEMDSIPENRVNFHKSSLDQIFETKTSDAVEDLFSEVDSSYYTLDTVLTGMMGNTKMGHCDVLETFPQGATNSNSNCKSDLNELDHFVEILVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2G6</accession>
    <name>A0A6I9X2G6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">U3 small nucleolar ribonucleoprotein protein IMP4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">IMP4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Component of a heterotrimeric complex containing IMP3, IMP4 and MPHOSPH10. Interacts with MPHOSPH10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908521.1">
        <property type="nucleotide sequence ID" value="XM_014053046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2G6"/>
    <dbReference type="GeneID" id="106538540"/>
    <dbReference type="KEGG" id="tsr:106538540"/>
    <dbReference type="CTD" id="92856"/>
    <dbReference type="OrthoDB" id="10253204at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000001">
        <property type="entry name" value="IMP4, U3 small nucleolar ribonucleoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF2">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="83"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013908521.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="33825" checksum="15BCEEDA0B00F5AD" modified="2020-10-07" version="1">MLRREARQRREYLYRKAQEEKLRTLQEKKEKIKKALDENCLIPTELRKEALVLQKALEFDDGGAEGVTSHVDDEYRWAGVEDPRIMVTTSRDPSSRLKMFAKEVKLVFPGAQRMNRGRHEVGALVRACKANGVTDLLVVHEHRGVPDGLIVSHLPFGPTAYFTLCNVVMRHDIPDIGTMSEANPHLIFHNFTSRLGQRATSILKYLFPVPKEDSKRVITFANKDDYISFRHHVYKKTDHRNVELSEVGPRFEMKLYMIKLGTLEQENTADVEWRWHPYTHTAKKRKFLSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3L5</accession>
    <name>A0A6I9X3L5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ankyrin repeat domain-containing protein 6-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106538469</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013908432.1">
        <property type="nucleotide sequence ID" value="XM_014052957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3L5"/>
    <dbReference type="GeneID" id="106538469"/>
    <dbReference type="KEGG" id="tsr:106538469"/>
    <dbReference type="OrthoDB" id="424503at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000273">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000370">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF185">
        <property type="entry name" value="ANKYRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="159"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="192"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="225"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013908432.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="35317" checksum="B47B5ACA497C023A" modified="2020-10-07" version="1">MGGHLSILQLTKVISMWSRFCSKQAVTWIFRMIPYWNPDAISDWSMSVHTAHAPEIVKGAVVLVTEVEEEKNKKKQRKKARKDPRRLQREKEGDQTALHRATVVGNTDVISALIHEGCALDRQDKDGNTALHEASWHGFSQSAKLLVKAGANVLARNKAGNTPLHLACQNNHSESVRVLLLGGSRTDSKNNAGDTCLHVAARYNHLPIIRLLLTAFCSVHEKNQVGDTALHVAAALNHKKVAKLLLEAGADGTVVNNAGQTPLEVAQEHNNPEAALLLTKAPQISRFNRGRSLRKKRERLKEERRAQSVPRDEVVQSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X691</accession>
    <name>A0A6I9X691_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein 708-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106539492</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909766.1">
        <property type="nucleotide sequence ID" value="XM_014054291.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539492"/>
    <dbReference type="KEGG" id="tsr:106539492"/>
    <dbReference type="OrthoDB" id="9047780at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001868">
        <property type="entry name" value="Zinc finger protein 264"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001532">
        <property type="entry name" value="Zinc finger protein 483"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000052">
        <property type="entry name" value="zinc finger protein 546 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013909766.1"/>
        </source>
    </evidence>
    <sequence length="269" mass="30630" checksum="214B54AA4E0FF191" modified="2020-10-07" version="1">MCLFFESELXYKCMECGKTFTQSSGLNNHKRIHTGEKPYKCMECGKTFALNGTLTIHKKFHTGEKPYKCIECGKTFAQSGHLICHKMVHTGEKPYKCMQCGKTFTQSSGLNKHKRIHTGEKPYKCMECGKTFTQSSGLNKHKRIHTGEKPYKCMECGKTFALKGTLAIHNKIHTGEKPYKCMECGKTFAQSGHLISHKMVHTGEKPYKCINCGKTFARSYELTSHNRIHTGEKPYKCMECGKTFAQNSNLISHKQFHTREKVYSNSNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6H2</accession>
    <name>A0A6I9X6H2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106540478</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911059.1">
        <property type="nucleotide sequence ID" value="XM_014055584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6H2"/>
    <dbReference type="GeneID" id="106540478"/>
    <dbReference type="KEGG" id="tsr:106540478"/>
    <dbReference type="OrthoDB" id="5831905at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050910">
        <property type="term" value="P:detection of mechanical stimulus involved in sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060005">
        <property type="term" value="P:vestibular reflex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006886">
        <property type="entry name" value="RNA_pol_III_Rpc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF17">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04801">
        <property type="entry name" value="RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="612"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="715"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="772"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="595"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013911059.1"/>
        </source>
    </evidence>
    <sequence length="825" mass="95228" checksum="EB8E78C026AD6106" modified="2020-10-07" version="1">MDTGYTQCLINMSIVEQLGICTRPLAHPIQFEQVDRMLIEGTAMRITELSLMGFESFEVFIVSPDLERNISPFKEMSPSWKKSTTCPKSTWLQRNQQPSQLGPEDVKDFLEHVSFLRINRGWEFKLRYDEDFVKKHPDVVQRQKMLWMGIQANSSSSTPNYSDDESLSEEELEKLKEQVEEKKKLISIIRNKPWCMAKRLTVLREAQDFVEKFEGALGKGKGKKLYAYKMMLTKKWVKFRRDFDNFKTQCIPWEMKIKEVESHFGSSVASYFIFLRWMYGLNLVLFGLIFGLVIIPEILMGMPYGRIPRKTVPRAEQPTAMDFSVLWDFEGYIKYSAIFYGYYNNQRTIGWLKYRLPMAYFMVGVIGVYGYSLMVVIRSMARNAHEDTGDGDDENFIFSWKMFTSWDYLIGNPETADNKFASITTSFKESIVDEQESNKDENIHLRRFLRILANVLIICCLCGSGYLIYFVVKRSQAFSKMQNIGWYERNEVEIVMSLLGMFCPPLFETIAALEDYHPRIGLKWQLGRIFALFLGNLYTFLLALMDDVKEKLTEEGVIRNITYWTVLTFHNSSSRNGSALIPPPMDPADVPRGPCWETTVGIEFVRLTVSDILVTFVMILVGDFLRACFVRFINYCWCWDLEAGFPSYGEFDISGNVLGLVFNQGMIWMGSFYAPGLVGINVLRLLISMYFQTWAVMSSNVPHERVFKASKSNNFYMGLLLLILFLSVLPVAYTIMSLPPSFDCGPFSGKKKMYDVIQETIKYDFPPIAAKVLGYLANPGLIITAVLLMILAIYYLNAVAEGYKHAHAELKKKMQMVFRFVYISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X780</accession>
    <name>A0A6I9X780_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mismatch repair endonuclease PMS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PMS2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013909697.1">
        <property type="nucleotide sequence ID" value="XM_014054222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X780"/>
    <dbReference type="GeneID" id="106539430"/>
    <dbReference type="KEGG" id="tsr:106539430"/>
    <dbReference type="CTD" id="5395"/>
    <dbReference type="OrthoDB" id="10254304at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016446">
        <property type="term" value="P:somatic hypermutation of immunoglobulin genes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="2">
        <location>
            <begin position="292"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013909697.1"/>
        </source>
    </evidence>
    <sequence length="437" mass="48397" checksum="5DA94650B9E648E5" modified="2020-10-07" version="1">MKSFLQNERDASPLETSACPLPPNRDEKSKIIARLKESFSRGKTPAFQEAKKAQPNHVPASQGLLQMFAGFSASSRKKILLHEESEDGSEVDGRNSPLAGRKLEKSDEDTDSGLGSISAESDGGLSRTPETAVHFRTESAAGSSGGEPRLFSGEIQSSEGGSGGVFWPSEPNQSEGDGFARQATSSPKAKRFKIRQPLSPKANFSAMTSEQDSGAQPLYDVPVKTARRTVSLAVPWSTLTNRIQKQLKRQETEGEMLKFRKFRAKINPGENKTAEDELRKEIRKEMFAKMDVVGQFNLGFIIAKLKSDLFIIDQHATDEKYNFEMLQQHTVLEGQKLIKPQNLNLTAVSESILMDNLEIFQKNGFDFVVDENAPVTQRVKLVSLPTSKNWTFGPQDVEELIFMLSDSPGIMCRPSRVRQMFASRACRKSVMIGTALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7G1</accession>
    <name>A0A6I9X7G1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sodium/glucose cotransporter 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106539822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910196.1">
        <property type="nucleotide sequence ID" value="XM_014054721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7G1"/>
    <dbReference type="GeneID" id="106539822"/>
    <dbReference type="KEGG" id="tsr:106539822"/>
    <dbReference type="OrthoDB" id="9809346at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF96">
        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910196.1"/>
        </source>
    </evidence>
    <sequence length="82" mass="9008" checksum="02CB5441FDDACF65" modified="2020-10-07" version="1">MSQTSATTESLNDKPEEKSFAFGVPDIIVVVIYFIFVLAVGIWSSLRASRGTVGGYFLAGRSMTWWPSLNIFLTASRSARNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8E5</accession>
    <name>A0A6I9X8E5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Delta-1-pyrroline-5-carboxylate synthase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="20">Glutamate 5-kinase</fullName>
                <shortName evidence="20">GK</shortName>
                <ecNumber evidence="20">2.7.2.11</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">Gamma-glutamyl kinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="20">Gamma-glutamyl phosphate reductase</fullName>
                <shortName evidence="20">GPR</shortName>
                <ecNumber evidence="20">1.2.1.41</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">Glutamate-5-semialdehyde dehydrogenase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="20">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="25" type="primary">ALDH18A1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 20">
            <text>L-glutamate + ATP = L-glutamyl 5-phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 20">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Can form homodimers/multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">In the N-terminal section; belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="20"/>
    <dbReference type="EC" id="1.2.1.41" evidence="20"/>
    <dbReference type="RefSeq" id="XP_013907598.1">
        <property type="nucleotide sequence ID" value="XM_014052123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8E5"/>
    <dbReference type="GeneID" id="106537852"/>
    <dbReference type="KEGG" id="tsr:106537852"/>
    <dbReference type="CTD" id="5832"/>
    <dbReference type="OrthoDB" id="1934954at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04256">
        <property type="entry name" value="AAK_P5CS_ProBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000010">
        <property type="entry name" value="Delta-1-pyrroline-5-carboxylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000011">
        <property type="entry name" value="Delta-1-pyrroline-5-carboxylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041744">
        <property type="entry name" value="G5K_ProBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005766">
        <property type="entry name" value="P5_carboxy_syn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01092">
        <property type="entry name" value="P5CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036429">
        <property type="entry name" value="P5C_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 20" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 20" id="KW-0521">NADP</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 20" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="23">
        <location>
            <begin position="115"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="22">
        <location>
            <begin position="406"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR036429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013907598.1"/>
        </source>
    </evidence>
    <sequence length="841" mass="92466" checksum="5BD4B3A32CE08104" modified="2020-10-07" version="1">MFFYLAVEVLFDVQWEKCTIQNHSGFPFFLLAGVLLFIKILTMLHRVALLPSLRSSGRFWQVSDVAGSHPCLLAPNLVLRNKSARGWSNIPFITVPLSRTHGKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSMLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGPNQLKDMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGNKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKISGHVITDIVEGKKVGTFFSEVKPAGPTVEQQAEMARTGGRSLAALQPEQRAEIIYHLADLLTDQKEEILRANKKDLEEAENKGRLALPLLKRLSLSTSKLNSLAIGLRQIAVSSQDSVGRVLRKTRIAKDLELEQVTVPIGVLLVIFESRPDCLPQVSALAIASGNGLLLKGGKEALHSNQILHHLTQEALSIHGVKDAVQLVNTREEVEDLCRLDKLIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHIYVDLEASIEKVTRIVRDSKCEYPAACNALETLLIHRDLLRTPVFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLECCIEVVDSVQDAIEHIHKYGSSHTDVIITENEKTADFFLQHLDSACVFWNASTRFSDGYRFGLGAEVGISISRIHARGPVGIEGLLTTKWLLRGENHIVSDFSEQGSMKYLHENLPIPQRNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X999</accession>
    <name>A0A6I9X999_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">adenylate cyclase</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106539925</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013910327.1">
        <property type="nucleotide sequence ID" value="XM_014054852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X999"/>
    <dbReference type="GeneID" id="106539925"/>
    <dbReference type="KEGG" id="tsr:106539925"/>
    <dbReference type="OrthoDB" id="2107370at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000002">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009398">
        <property type="entry name" value="Adcy_conserved_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF11">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06327">
        <property type="entry name" value="Adcy_cons_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="229"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="660"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="18">
        <location>
            <begin position="370"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="575"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="576"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013910327.1"/>
        </source>
    </evidence>
    <sequence length="684" mass="77465" checksum="61FB60E1CE0F7DB8" modified="2020-10-07" version="1">MSWFSGILVPKVDERKTAWGERNGPKRHRPALCGPRYMSCLQDQELERRGSGALALNCSWQEDHYGRKQPPSLPPGSGTGDLGLKAVDLGFEDGGGMAVEAKRLSVSDCGWHLLQVFRSKRFRSAKLERLYQRYFFQMNQSSLTVLMGVLVLACGIMLLFHCVPGTPFVSYTTALAVAATLFLSLMVLCNRTGFHQNCMWVVSYLVIVALCGVQALGTLKVSPRSASEGVWWSIFFIYIIYTLLPVRMRVAVLAGALLSVLHLVISWYQNNTDSFVWKQLVANALIFLCTNIIGICTHYPAEVSQRQAFRETRGYIQARLHLQRENRQQERLLLSVLPQHVAMEMKEDINTKKEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITQATLQYLNEDYEVEPGHGGERNAYLKEHNIETFFIVGCSQKRKEEKAILAKLQRAQANSTEGLVPRWVPERSFPRNKDSKALRQMGIDDSSKDNRSTQEALNPEDEVDEFLGRAIDARSIDQLRKDHVKRFLLTFQKPELERKYSKKVDTRFGSYVACTMLVFCCICCIQIVIFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBQ6</accession>
    <name>A0A6I9XBQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106540617</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013911277.1">
        <property type="nucleotide sequence ID" value="XM_014055802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBQ6"/>
    <dbReference type="GeneID" id="106540617"/>
    <dbReference type="KEGG" id="tsr:106540617"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000048">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF20">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013911277.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="33053" checksum="C3EC88CCBE69D6C2" modified="2020-10-07" version="1">MREDRAMSWEQLQQCIMGKMRYLMRREAQVQASGSLFQVRVVGGSSLCSYLSPQDRRPLYHPAVDRALQFGGPGGPVHVKLVVEWDLTTKECLFGNIQEEVVEDAESVRMKQQLHQQQHSCTLSECFQLYTKEEQLAPDDAWRCHHCQVLQQGMVKISLWTLPDILIIHLKRFRQVGEQRHKLSTLVHFPLRGLDMAPHVAKRSRGSKWGPWRQPLPEEGQCNFLYDLYAVCNHHGSLHGGHYTAYCCNSLDGRWYSYDDSTVEPVMEEDISTRGAYILFYQRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC88</accession>
    <name>A0A6I9XC88_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flavin reductase (NADPH) isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BLVRB</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911876.1">
        <property type="nucleotide sequence ID" value="XM_014056401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC88"/>
    <dbReference type="GeneID" id="106541075"/>
    <dbReference type="KEGG" id="tsr:106541075"/>
    <dbReference type="CTD" id="645"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004074">
        <property type="term" value="F:biliverdin reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05244">
        <property type="entry name" value="BVR-B_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051606">
        <property type="entry name" value="Polyketide_Oxido-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43355">
        <property type="entry name" value="FLAVIN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43355:SF2">
        <property type="entry name" value="FLAVIN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="76"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013911876.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="28641" checksum="1A01A0A5FB64EB58" modified="2020-10-07" version="1">MPRWVCGSRRVVTLRPSLTSARPPASPREPRVPVGTRGSAALASRGCECRGGASGGSRGCGGKKRSNARHLGGREGARQAAAALAGYQVTVLVRDPARLPPDLQPAQVVVGDVLNRADVDRAVQGQDAVIIVLGTRNDLSPTTMMSEGTRNIVAAMKSCSIGKVVVCLSAFLMWDPDKVPARLRPVTEDHIRMQQILKESGLDCVYVMPPHIAGDQPLTGDYTVTVNTSSGARVISKHDLGHFFLKCLTTSEYDGKNVYLSRHYAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD86</accession>
    <name>A0A6I9XD86_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ATP-binding cassette sub-family D member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ABCD1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909123.1">
        <property type="nucleotide sequence ID" value="XM_014053648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD86"/>
    <dbReference type="GeneID" id="106538983"/>
    <dbReference type="KEGG" id="tsr:106538983"/>
    <dbReference type="CTD" id="215"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000800">
        <property type="entry name" value="ATP-binding cassette sub-family D member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF21">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="488"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909123.1"/>
        </source>
    </evidence>
    <sequence length="743" mass="82730" checksum="AA6AAB79A9DA4291" modified="2020-10-07" version="1">MPFAPAKVATRLSQGITAKRAAVALLLAAYGARKLYPILRRQWAASVLQVDAETASPRESNAKWGELEVDGASSSSSPPGVNKVFFLRLLRLLRLLFPGPLCRQTGLLALHSAALASRTFLSVYVAQLDGRLARSIVRKNPRDFAWQLLQWLLIALPATFVNSAIRYLEGQLSLAFRGCLVDHAYRLYFEGQTYYRVSNMDGRLRNPDQSLTEDLVAFANAVAHLYSNLTKPVLDLIVTSYTLVSSARSKGADTAWPSLIAGLVVCITAKVLRACSPKFGQLVAEEARQKGELRYMHSRVVANSEEIAFYGGHKVELSLLRHCYHQLASKINVLLLERLWYVMLEQFLMKYVWSAAGLVMVAVPIITATGYSETDSEAVKQAALEMDEQELVSLRTEAFTTARSLLAAGADATERVISSYKEATELAGYTARVYEMFQVFEDVKCCNFRRPGELDEGQAQAGTVMKHGMRVEGSLQIQGHVIDVDHGIVCENIPIITPTGDVVVTSLNIRVDEGMHLLITGPNGCGKSSLFRILGGLWPTYNGTLYKPPPHRMFYIPQRPYMSVGTLRDQVIYPDTAKDMGRKGHTDSDLERILDIVNLNYIVQREGGWEAISDWKDVLSGGEKQRMGMARMFYHRPKYALLDECTSAVSIDVEGKIFQAAKDAGIALLSITHRPSLWKYHTHLLQFDGEGGWRFEKLDPEARLTLREEKQRLEQQLAGVPEMQQRLSELCQILGEDAPSPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDC4</accession>
    <name>A0A6I9XDC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adipolin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Adipose-derived insulin-sensitizing factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Complement C1q tumor necrosis factor-related protein 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FAM132A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adipolin/erythroferrone family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911862.1">
        <property type="nucleotide sequence ID" value="XM_014056387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDC4"/>
    <dbReference type="GeneID" id="106541059"/>
    <dbReference type="KEGG" id="tsr:106541059"/>
    <dbReference type="CTD" id="388581"/>
    <dbReference type="OrthoDB" id="6431870at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046326">
        <property type="term" value="P:positive regulation of D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046628">
        <property type="term" value="P:positive regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000012">
        <property type="entry name" value="Adipolin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052136">
        <property type="entry name" value="Adipolin/Erythroferrone-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24019">
        <property type="entry name" value="ADIPOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24019:SF5">
        <property type="entry name" value="ADIPOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0372">Hormone</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027080481" description="Adipolin" evidence="12">
        <location>
            <begin position="26"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="13">
        <location>
            <begin position="108"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013911862.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="27605" checksum="F8767C5BA88B9A02" modified="2020-10-07" version="1">MSPKERRRYWGWLVLAMLICQESPSFPRVTAAKREPRKKPKDPNQYTGALPNTTLSNSEELNGHAKGNRICNPFFHPEATERRASPVLPAHPSEIPVILPPLEEISFYRRVEEAFHCKLKGQIIVDKKTLMELQNFQMPLAKGAFLRGSGLNLTTGRFTASVSGIYQFSANVHVDHSELKSKLQLRARDNVRVLICIESLCHRYTSLEVIAGLESNSKVFTVYVHGLLQLQILLMLVPMDDVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEA8</accession>
    <name>A0A6I9XEA8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="7">PARP</shortName>
            <ecNumber evidence="7">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106542699</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013913979.1">
        <property type="nucleotide sequence ID" value="XM_014058504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEA8"/>
    <dbReference type="GeneID" id="106542699"/>
    <dbReference type="KEGG" id="tsr:106542699"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000008">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052056">
        <property type="entry name" value="Mono-ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453">
        <property type="entry name" value="PARP/ZINC FINGER CCCH TYPE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453:SF89">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PARP catalytic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013913979.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="19139" checksum="12ADF632192C6CDE" modified="2020-10-07" version="1">MWNHIEKLSGIRATIERVQNPYLWQSYQVKKKEIDTQNGHKNNEKVLFHGTPRSTLVQIYQTGFNRSYAGKNAAAIGNGTYFAVKSNYSAQDTYSTPDQSGRKYMYVARVLTGDYCIGRSGQIIPPSKNKAGFDLYNSVTDNMNRPSMFVIFHDAQAYPEYLITFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFU6</accession>
    <name>A0A6I9XFU6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SCAN domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106539627</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909928.1">
        <property type="nucleotide sequence ID" value="XM_014054453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFU6"/>
    <dbReference type="GeneID" id="106539627"/>
    <dbReference type="KEGG" id="tsr:106539627"/>
    <dbReference type="OrthoDB" id="9013560at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="192"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013909928.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="32214" checksum="AA1EF05E0BC831D3" modified="2020-10-07" version="1">MAMEEGALLDLAPAIHARPKPEVTLDEGVEEQEEGETDVPAEEEMVGWEDTWEETTDAGAGVKSSERTGGFPAGGTRAFLMSSPRDFGKELGLGVPNSEESRWKDFLKTVEAPGLSCSSLQELSSSLHNTSATLPASVKVKAEGTQFTVERETQTQPGLSGESQEPHGNPKPSEPPKEELPEADTVVSEIQRQHFRQFLYREAAGPREACSRLWFLCCRWLKPERHTKEQILELLILEQFLAILPSDLQSWVVEGCPQTCAQAVALAEGFRQKEWVPETLDEQVRRTRGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFU9</accession>
    <name>A0A6I9XFU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein SERAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Serine active site-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SERAC1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the SERAC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913041.1">
        <property type="nucleotide sequence ID" value="XM_014057566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFU9"/>
    <dbReference type="GeneID" id="106541967"/>
    <dbReference type="KEGG" id="tsr:106541967"/>
    <dbReference type="CTD" id="84947"/>
    <dbReference type="OrthoDB" id="5086500at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913041.1"/>
        </source>
    </evidence>
    <sequence length="660" mass="75286" checksum="E9CBEF20BC4FCB47" modified="2020-10-07" version="1">MGRMSLAAYCVVCYRKIGTLGSTWKVDQSWKDIRKIVKLTGSLILGGCIFVTYEVLTLRKLFSFDTQVVQQEKLKSYIYLQTVSLDQSDYQGVIYQVRKKLHRAAKKIIEMHARIFQKPFDERFSTLDAEERDYALWLLVKRSQSNDKMVRLQAVQELAANRYWHDYQYRTVAQTSDQRMAIALARSKDVDLRFFLPPHLLLNPKDTCCLEDELRLLLVSLPQTDLDQCVQYFTSLALRESSQTIAAQKGGLWCFGGNGLPYCESLSTIPAKTVELFFLQALVEHSKIPSHCNKIEANKGLQLLQKLYHLHRDSRKIQRSIIHIIGNMALDEHLHASITRAGWVSILAAMMKSPRVMESSHAARALANLDREVVQEKYPDGVYILHPQCRTNEPIKADVLFVHGLLGAAFKTWRQQDLEQSADDRDMDAEDDYSECWPKTWLASDCPSLRIISVEYDTHLSDWRTKCPVESYRESLAYRSTELLKKLRAAGIGDRPLIWLSHSMGGLLVKKMLLDASMDPEMAPIVDSTQGIVFYSVPHHGSRLAEYSVNIRFLLFPSVGVKELSKDSPALKALNDDFISFAKNQNFPVLSFAETLPTRVGRMLSLHVVPVESADLGIGELIQVEVSHLNICKPKNKESFLYQQTLKFIQESLKRQLGNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHU8</accession>
    <name>A0A6I9XHU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106542454</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013913661.1">
        <property type="nucleotide sequence ID" value="XM_014058186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHU8"/>
    <dbReference type="GeneID" id="106542454"/>
    <dbReference type="KEGG" id="tsr:106542454"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046356">
        <property type="entry name" value="MARCHF4/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="32"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013913661.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="29589" checksum="D3F808EB6021E74F" modified="2020-10-07" version="1">MFDFTTQINIGYFRAHTYIYGYLPEFLRTFQGELLNPCRCDGSVRYTHQLCLLKWISERGSWTCELCCYRYHVIAIKMKRPCQWQSITVTLVEKVQMIAVILGSLFLVASVTWLLWSAFSPYAVWQRKDLLFQICYAMYGFMDLVCIGLIVHEGASVCRVFKRWRAVNLHWDVQNYDKATDIEESTRGESSTARTLWLPLNALRNRNLVHSTQLTSPRFQCGYVLLQLFTRMRPPEDSSDDNSSGEVVMRVTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIC2</accession>
    <name>A0A6I9XIC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GTPase-activating protein SYDE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SYDE2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913806.1">
        <property type="nucleotide sequence ID" value="XM_014058331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIC2"/>
    <dbReference type="GeneID" id="106542552"/>
    <dbReference type="KEGG" id="tsr:106542552"/>
    <dbReference type="CTD" id="84144"/>
    <dbReference type="OrthoDB" id="120383at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000031">
        <property type="entry name" value="rho GTPase-activating protein 100F isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052118">
        <property type="entry name" value="Rho-GAP_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46150">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 100F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46150:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN SYDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913806.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="38901" checksum="C8C074ED1D8705EE" modified="2020-10-07" version="1">MLKVVGLYRLCGSAAVKKELREAFEKDSKAVTLCETQYPDINVITGILKDYLRELPSPLITKQLYEAVLDAMVKRPLKMTANGCENDCNDSEYTMALLDCLPEVEKATLKMLLDHLKRVASYHEVNRMTCQNLAVCFGPVLLSQWQETTSHNNRVFTDSEELASALDFKKHIEVLHYLLQLWPVQHPSTEESALLEQQSSVNYLRPKKQRPQMLNLSEVSGILRSRPARLDSPSSNRYAGDWTACGENYFVGPKEALSEADYDDVPSEEAESGDDGSQADSSRTLLRRPICVSKERTFQAYLTMQTMDSAVNHQVNLKNLQESIDTLIGNLERELNKNKLNMSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKI5</accession>
    <name>A0A6I9XKI5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-directed RNA polymerases I, II, and III subunit RPABC4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA-directed RNA polymerase II subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">POLR2K</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916314.1">
        <property type="nucleotide sequence ID" value="XM_014060839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKI5"/>
    <dbReference type="GeneID" id="106544543"/>
    <dbReference type="KEGG" id="tsr:106544543"/>
    <dbReference type="CTD" id="5440"/>
    <dbReference type="OrthoDB" id="5585087at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039747">
        <property type="entry name" value="RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12056">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12056:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03604">
        <property type="entry name" value="Zn_ribbon_RPAB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013916314.1"/>
        </source>
    </evidence>
    <sequence length="65" mass="7791" checksum="B9C2CED241CD6415" modified="2020-10-07" version="1">MERAGFQQDMEEDAPPPKQQPMIYICGDCHKENEIKARDPIRCRECGYRIMYKKRTKRLVVFDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMS1</accession>
    <name>A0A6I9XMS1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">Cyclin-dependent kinase inhibitor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8 9" type="primary">CDKN1A</name>
    </gene>
    <organism evidence="5 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912060.1">
        <property type="nucleotide sequence ID" value="XM_014056585.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912061.1">
        <property type="nucleotide sequence ID" value="XM_014056586.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912062.1">
        <property type="nucleotide sequence ID" value="XM_014056587.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912063.1">
        <property type="nucleotide sequence ID" value="XM_014056588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMS1"/>
    <dbReference type="GeneID" id="106541210"/>
    <dbReference type="KEGG" id="tsr:106541210"/>
    <dbReference type="CTD" id="1026"/>
    <dbReference type="OrthoDB" id="9940972at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072331">
        <property type="term" value="P:signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029841">
        <property type="entry name" value="CDKN1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46778:SF1">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46778">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="4">
        <location>
            <begin position="20"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="77"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013912060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013912061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013912063.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="17264" checksum="921B8C98E82BE88A" modified="2020-10-07" version="1">MPLSESKILQPSCSRKLCRNLFGPVDHEQLQKDFQSLMKVQLEEAQQKWNFDFETETPLEGNYKWEKVSHLNMLPSPDLLEHTKENSCTGEKSRSPPALFKRHTKTERPGQVGLEACKADSPRCLKRKQTSIKDFYGSKRRTSPYKPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNC9</accession>
    <name>A0A6I9XNC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Plexin-C1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106545417</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917444.1">
        <property type="nucleotide sequence ID" value="XM_014061969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNC9"/>
    <dbReference type="GeneID" id="106545417"/>
    <dbReference type="KEGG" id="tsr:106545417"/>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF4">
        <property type="entry name" value="PLEXIN-C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026833667" evidence="6">
        <location>
            <begin position="45"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="905"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="8">
        <location>
            <begin position="54"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="7">
        <location>
            <begin position="427"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="7">
        <location>
            <begin position="566"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013917444.1"/>
        </source>
    </evidence>
    <sequence length="999" mass="112797" checksum="9A5E0C33EF1453AA" modified="2020-10-07" version="1">MVRRSGGTRRYLARRDPGSSALAAPPPPLLLLLLLAWAAAGGRGQGPLEFPHAVANLAVSGERFLVASGNCWYEVAPSLAWKEKRFCEKSGTSCGESVASVNKLLLPFSRDRLLTCWTQPQGVCRLQNLADVSLNETLGEELVTYASESAVVGLMYCEDDKDSRWSLVAATTHKLNNCPESPPASHANKAVFLIKEGEERVAENRQVAMKNVYNLHFEDAFRWQKHFFFPYHNNDSMQAKMLIALYERNTFRHNSQILRCGGKTKILSSAYLEQRGLWAGIFGSASSPATPTSTGLCIFALEKLLENANNCTFRQPPASEFSSEDDASDKCINVTWPIKNSTSLSHFNLVSVYATVVLNKAVLFLGTGNGQLLKIVLDDELKPNCPEILYEIKEETPIFHRLELDPTDQNYIYLPSNNMIRRIQVANCNKYISCRDCLSAMDPHCGWCHTKKSCTLKGECSFSSNSPNWVNISYGKDKCLKIHTSDNLHFKNNKIPVTVDVNSFGLSESRSRCRMLNARTNEILCDVNQHHLNCSCDINFKDLSDKVQVLFISGTWNLSEIFEFDICSLSKTCSKCNHMHCIWNAGEKKCVTSTMTCAKKIDCSSTINALNKKDRSATSKPIKIISIEPNYVSTLGKPEVLVIGENFTKSNFLMEITGTSSCQRDVGYVTKVLNGTHMKFCLPPSRKEVKSACIKEDGFECSASFPLHYVSFPSCTKILPNITWMSGGRNMTIHGKYLNIIDEIILSDFPQSIMQFTCHKNSYECSFTTPAIKMEKGSKIINIISKVENINISCGYFQYYPDPEFIHYELITDLEPDLELKIHKTKDNLNLSRHELEVFISDENLVNITFSVQNISNTATRSIIHCRAKQEKTLTRKINKSNVKVYVKVGNFVHEVPTEKKNYPFLYILLLIPIVIAVFLVAVFVTQRKSKQLNQKLSEHLELLECELRKEIREGFVELQVEELDIVDSFGTIPFLDYKHFVLRTFFPEVNDYLSKTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNZ3</accession>
    <name>A0A6I9XNZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gamma-1-syntrophin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Syntrophin-4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SNTG1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Adapter protein that binds to and probably organizes the subcellular localization of a variety of proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. May participate in regulating the subcellular location of diacylglycerol kinase-zeta to ensure that diacylglycerol is rapidly inactivated following receptor activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the syntrophin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915591.1">
        <property type="nucleotide sequence ID" value="XM_014060116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNZ3"/>
    <dbReference type="GeneID" id="106543991"/>
    <dbReference type="KEGG" id="tsr:106543991"/>
    <dbReference type="CTD" id="54212"/>
    <dbReference type="OrthoDB" id="9975356at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016013">
        <property type="term" value="C:syntrophin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06801">
        <property type="entry name" value="PDZ_syntrophin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000448">
        <property type="entry name" value="Syntrophin gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000080">
        <property type="entry name" value="Syntrophin gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015482">
        <property type="entry name" value="Syntrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055108">
        <property type="entry name" value="Syntrophin_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554:SF2">
        <property type="entry name" value="GAMMA-1-SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554">
        <property type="entry name" value="SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23012">
        <property type="entry name" value="Syntrophin_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="64"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013915591.1"/>
        </source>
    </evidence>
    <sequence length="537" mass="60262" checksum="083B29577AEF551E" modified="2020-10-07" version="1">MDFKTACEELLGRVYKSKTGICLLQDGNQEPFKVRLHLAKDILTIQEQDVICVSGEPFYSGERTVTIRRQTVGGFGLSIKGGAEHNIPVVVSKISKEQRTELSGLLFIGDAILQINGINVRKCRHEEVVQVLRNAGEEVTLTVSFLKRAPAFLKLPLNEDCACVPSDQSSGTSSPLCDSGLHLNYHPNNTDTLSCSSWPTSSGVRWEKRWCDLRLIPLLHSRFSQYLPGSDMCRQNAFQVVAVDGICSGIIQCLCAEDCIDWLQAIAINISNLTKHNIKKINRNFPVNQQIVYMGWTEEREQDSLQDRMYTPKFLALRGSSLYKFLAPPVTTWDWTRAERTFSVYEIMYKIFKDSDILDRRKHCFGVQSEMGEDLYFSVELECDLILWEKAFQSATFLEVERIQCKTYACVLESHLMGLTIDFGMGFVCFDAATKAVLWRYKFSQLKGSSDDGKSKIKFLFQNPDTKQIEAKELEFSNLFAVLHCIHSFFAAKVACLDPLYVGSQATTATTSASVASSSSATTTATSSGSCKLKYMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ14</accession>
    <name>A0A6I9XQ14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel subfamily A member 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106541779</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912793.1">
        <property type="nucleotide sequence ID" value="XM_014057318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ14"/>
    <dbReference type="GeneID" id="106541779"/>
    <dbReference type="KEGG" id="tsr:106541779"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000021">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000053">
        <property type="entry name" value="potassium voltage-gated channel subfamily A member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF250">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="11"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013912793.1"/>
        </source>
    </evidence>
    <sequence length="471" mass="53142" checksum="FA1645AC9F043EE6" modified="2020-10-07" version="1">MESAGEGELAERVLLNVSGLRFETRLGTLDQFPETLLGDPAKRLRYFDPLRNEYFFDRHRPSFDGILYFYQSGGKLRRPVNVSLEVFADEVRFYQLGEAAMERFREEEGFLREEEKPLPTHEFQRQVWLIFEYPESSGSARGIAIVSVLVILISIVTFCLETLPEFQEEPAEIRDPTGQTPRPSILADPFFVIETTCVIWFTFELLVRFFACPSKPEFSKNIMNIIDVVAIIPYFITLGTELAHEQQKGEPQGNSGGQQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADDPESYFSSIPDAFWWAVVTMTTVGYGDMSPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETDQSLLQDDPGSVRGSSDDEVKSKNSSCKSVVHLEHLDETHNGTRSVEKTTIKANSTLDLRKSLYALCMDSTRETDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQK7</accession>
    <name>A0A6I9XQK7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chondroitin sulfate proteoglycan 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106546076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918329.1">
        <property type="nucleotide sequence ID" value="XM_014062854.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546076"/>
    <dbReference type="KEGG" id="tsr:106546076"/>
    <dbReference type="OrthoDB" id="9026019at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039005">
        <property type="entry name" value="CSPG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051561">
        <property type="entry name" value="FRAS1_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739:SF12">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 4-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16184">
        <property type="entry name" value="Cadherin_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51854">
        <property type="entry name" value="CSPG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="142"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="355"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="823"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1057"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1169"/>
            <end position="1267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013918329.1"/>
        </source>
    </evidence>
    <sequence length="1408" mass="160002" checksum="A71EDC396F012929" modified="2020-10-07" version="1">MKRHLEATTGLRSVRINFRTNEQILRSQLHSQLDDLEWHLLEIRHKRENITLVIDKNYQTSAIVPDFLVFELNIEYGLYIGGRGTLDTHYLNSTPINFRGCINDVIFNNHDILTSLKSSARNKNIHEVSLSCSNEFFAGEEEPVSFFSSKSYVSFPLWNVQAGGILQYSVQTTAQRGLLIYSSGPVGDFIAMEIENGLIKAHIRMGKNRLELSSLSPVNDNQWHVIRLKFSIKYVELTLEKKIVKTPLPSHSRLPVLKGALHVGGINDALRAEVLKYGLASVSGRYGEGGSFRGCLKDLIIGSQKYSLKNVLVTKDISVGCTTPNFAPKKQAVGIAPSISVAGTVAPKLLRKESHEHFLFVDDLIVPEGGQAFLGSKHIKVNVDFKTLGIRQSQILFRIIHPPSYGQLRLNVVSVPEDFTFSMLDLWQGKVLYIHDGSEESYDYFTFSVSTSSKREMPLYLQGNDEYMFTITVKPINDAPELIFPNGNLFLLLEHSKKCLSIDSVNIVDNDTSAADLSITVLGNSIADAGFLENSKTPGETINTFSYEDLQHGNVFFVHSGVQNSRIVLRASDGEKVSNTAVLRVMAVPLDYKLVNNTGVDVVQGTPVLITLSHLATEINAFQQELDIRYEITEEPFSGEIQRRHTGREWKKTNYFSQRSIERGRVRYHSTFKAIQQDNVYEHFKFKIIIGSNISQEFLFAVRVKWLTYNFLKYAPLVIDKLDRECLNSNNLLVVIPQMDLSENEILYKLQSLPEKGIILLDNVPLQINSSFSQQNISDCKVEYQLYRETHEESLDTFQFFISTQYVVSNIYDFKIKIKTNPQNIILINNGLTVTEGEGSLITKSELFVQTSINTTFEYKVTKSPQHGKLMLINFSDSPESNNNLSSFTNQDIYGEHLMYIHDDSETQCDEIHITATAIKFGEEDLETEFPLSVGIKFNISIHLKNDEKPVRVVDKVFHVVKNGRKLLTLDDLCYHDPDTDFDDNQLLYTRRGIPNGDLVLVNETSYRLYQFKQEDLIHKQVLFVHRGATYGRFVLFVTDGKHYTSSLLEVSASEPYVFLANNTGLLIQKGKEAVITTTNLSATTNQDIRSDHEIRFEIFSSPKYGQIVVNNLAVSTFTQHDLKMGHLTYRHDDSNNLIDTLNFTVYAKSLHLDADMNIHIYLESHQRPPVVLHNHKLLVEEGKRVQINNGKLMAVHENSLPSEIEFKVRSPPVYGYLWMFISKENYLGAEENPVLSFTQQDINDGIIQYIQTISNQLQDHFSLDVTNGIQTVSGIEVTVDIIPKLIPLEVHNFTVAEGGSKVLEEDILKISNSHFAEVNCDFSLIEPPIYGRIVNSHFWGMTLTKFNRKQARNTSLPPPFIMRFSLQTRFPGSHHPFFLLWRPELEAIFQVWSHRCGVKHCYHSLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRR0</accession>
    <name>A0A6I9XRR0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Frizzled-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">FZD9</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913388.1">
        <property type="nucleotide sequence ID" value="XM_014057913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRR0"/>
    <dbReference type="GeneID" id="106542247"/>
    <dbReference type="KEGG" id="tsr:106542247"/>
    <dbReference type="CTD" id="8326"/>
    <dbReference type="OrthoDB" id="5959102at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15036">
        <property type="entry name" value="7tmF_FZD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07463">
        <property type="entry name" value="CRD_FZ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000007">
        <property type="entry name" value="Frizzled class receptor 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000020">
        <property type="entry name" value="Frizzled class receptor 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
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        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
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        <property type="entry name" value="Frizzled_dom"/>
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    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041777">
        <property type="entry name" value="FZ9_CRD"/>
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    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
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    <dbReference type="PANTHER" id="PTHR11309:SF79">
        <property type="entry name" value="FRIZZLED-9"/>
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    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Frizzled"/>
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    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
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    <feature type="chain" id="PRO_5026789068" evidence="17">
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            <begin position="29"/>
            <end position="598"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="243"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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    <feature type="transmembrane region" description="Helical" evidence="16">
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <end position="439"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="460"/>
            <end position="481"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="520"/>
            <end position="539"/>
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    </feature>
    <feature type="domain" description="FZ" evidence="18">
        <location>
            <begin position="46"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="19">
        <location>
            <begin position="243"/>
            <end position="546"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="170"/>
            <end position="208"/>
        </location>
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        <location>
            <begin position="51"/>
            <end position="112"/>
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    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="59"/>
            <end position="105"/>
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    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="96"/>
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    <feature type="disulfide bond" evidence="14">
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            <begin position="123"/>
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    <feature type="disulfide bond" evidence="14">
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            <begin position="127"/>
            <end position="151"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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    <evidence type="ECO:0000256" key="14">
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            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
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    <evidence type="ECO:0000256" key="15">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013913388.1"/>
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    </evidence>
    <sequence length="598" mass="66134" checksum="808E264A6AF88E2B" modified="2020-10-07" version="1">MAAPVLRAGLLLLLLRAIAALLCQLVVAGSTLELLGGGGVYEAERGRGGRCQAIEIPMCQGIGYNLTRMPNLLGHEHQREAALKLEEFAPLVAYGCHGHLRFFLCSLYAPMCTDQVSSSIPACRPMCEQARQRCAPIMEHFHFGWPDALDCSRLPTKNDPHALCMEAPENATTAEPHRGQGMLPVAPRPARPSGDGPSESRPGSSASCENSDKFQYVAKSLSCAPRCSPGVDVFWSRQDKDFAFIWMAVWATLCFVSTAFTVLTFLLEPHRFQYPERPIIFLSMCYNVYSVAFIIRSVAGAENIACDRENGELYIIQEGLESTGCTIVFLILYYFGMASSLWWVILTLTWFLAAGKKWGHEAIEAHSSYFHMAAWGIPAMKTIVILTMRKVAGDELTGLCYVGSMDVNALTGFILIPLSCYLVIGTSFILTGFVALFHIRKIMKTGGTNTEKLEKLMVKIGVFSILYTVPATCVIVCYFYERLNMDYWNLQALEHSCLPFPGHRSVDCSLDASVPTMAVFMLKIFMSLVVGITSGVWVWSSKTLQTWQSLCNRKLGVRTRGKPCSGVTCTGAHCHYKAPAVMLHMTKTDPYLDNPTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSG7</accession>
    <name>A0A6I9XSG7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Feline leukemia virus subgroup C receptor-related protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">FLVCR2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>choline(out) = choline(in)</text>
            <dbReference type="Rhea" id="RHEA:32751"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ethanolamine(in) = ethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:32747"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>heme b(in) = heme b(out)</text>
            <dbReference type="Rhea" id="RHEA:75443"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the major facilitator superfamily. Feline leukemia virus subgroup C receptor (TC 2.A.1.28.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917022.1">
        <property type="nucleotide sequence ID" value="XM_014061547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSG7"/>
    <dbReference type="GeneID" id="106545087"/>
    <dbReference type="KEGG" id="tsr:106545087"/>
    <dbReference type="CTD" id="55640"/>
    <dbReference type="OrthoDB" id="422206at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015220">
        <property type="term" value="F:choline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097037">
        <property type="term" value="P:heme export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000101">
        <property type="entry name" value="feline leukemia virus subgroup C receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000092">
        <property type="entry name" value="Feline leukemia virus subgroup C receptor-related protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF3">
        <property type="entry name" value="HEME TRANSPORTER FLVCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="16">
        <location>
            <begin position="43"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013917022.1"/>
        </source>
    </evidence>
    <sequence length="485" mass="53278" checksum="2CC9E3471B939C8C" modified="2020-10-07" version="1">MGCLEKPDIVQEAKPELVEAEAPNGSIPALVPEVRLTWRRWVIVVLFSSYSFCNAFQWIQYGSINLIFADFYGVTHLAVDWLSMCYMVVYIPLLFPVAWLLDLKGLRLIALAGSALNCLGAWVKTGSAEPHLFPVTILGQVICSLAQVFILGMPSRIASVWFGPREISSACSIAVFGNQLGIAAGFLLPPVLVPDLKDKEELAYHIGIMFFITAGVATALFILVVFVFQEKPPYPPSPAQAVIKAAAPEEYSYLKSIVRLFSNVNFVLLIVTYGLNTGCFYALSTLLNRMVLLHYPGENLNAGRIGLTIIVAGMFGALISGIWLDRTKTYKQTTLVVYIMSFVGLVVYTFTLDLGHIWVVFITAGVLGFFMTGYLPLGFEFAAELTYPESEGTSSGLLNVSAQVFGIIFTISQGHIIDNFGTKAGNIFLCIFLFIGAIITAFIKPDLRRQQANLDNEQARLQSSNSQIMNYGFCTLSPSFPILAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVZ2</accession>
    <name>A0A6I9XVZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Pre-mRNA-splicing factor 38A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PRPF38A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in pre-mRNA splicing as a component of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the spliceosome B complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the PRP38 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914813.1">
        <property type="nucleotide sequence ID" value="XM_014059338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVZ2"/>
    <dbReference type="GeneID" id="106543342"/>
    <dbReference type="KEGG" id="tsr:106543342"/>
    <dbReference type="CTD" id="84950"/>
    <dbReference type="OrthoDB" id="190958at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071005">
        <property type="term" value="C:U2-type precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024767">
        <property type="entry name" value="PRP38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12871">
        <property type="entry name" value="PRP38_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 38 C-terminal" evidence="11">
        <location>
            <begin position="180"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="183"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="225"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914813.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="37435" checksum="D4FDB2617347B6E3" modified="2020-10-07" version="1">MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTAELVVDKAMELRYTGGVYGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFKYVRLLGALYMRLTGTAIDCYKYLEPLYNDYRKIKSQNRNGEFELMHVDEFIDQLLHDERVCDIILPRLQKRYVLEEAEQLETRISALEEDMDDVESSEDEEEDDEKLDRAPSPDHRRRGYRDLDKPRRSPTLRYRRSRSRSPRRRSRSPKRRSPSPRRERHRSKSPRRHRSRSRERRHRSRSKSPGHHRSHRHRSHSKSPERSKKSHKKSRRGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWQ7</accession>
    <name>A0A6I9XWQ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A</fullName>
            <ecNumber evidence="2 13">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TRMT61A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>adenosine(58) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.220"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(1)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55392"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12415"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2 13"/>
    <dbReference type="RefSeq" id="XP_013915068.1">
        <property type="nucleotide sequence ID" value="XM_014059593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWQ7"/>
    <dbReference type="GeneID" id="106543548"/>
    <dbReference type="KEGG" id="tsr:106543548"/>
    <dbReference type="CTD" id="115708"/>
    <dbReference type="OrthoDB" id="1925287at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.20:FF:000002">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000097">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="15">
        <location>
            <begin position="64"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="114"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR017269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013915068.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="33870" checksum="EE8E09983B67B56A" modified="2020-10-07" version="1">MSFIEYGDTIKEGDTAIVFLGHESMFPVKVQHGGKTQTKYGVIRHSTDLIGKEYGSKVTCSKGGWVYILYPTPELWTLNLRHRTQILYSTDISLITMMLELKPGSIVCESGTGSASLSHAIIRTIAPTGHLYTVEFHQQRAEKAIEEFEEHKVGHLVTVKTQDVCKNGFGVTNIADAVFLDVPSPWEAVGHAKLALKHEGGRICSFSPCIEQVQRTCLALEEHGFTEITTSEILLRVYNVRTISLQIPDLGKTADGNSSTGVDGGSLSHEDALCPNLQQETIQFKSGVPLKEMVGHTGYLTFATKNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWZ6</accession>
    <name>A0A6I9XWZ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CDK3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013915148.1">
        <property type="nucleotide sequence ID" value="XM_014059673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWZ6"/>
    <dbReference type="GeneID" id="106543614"/>
    <dbReference type="KEGG" id="tsr:106543614"/>
    <dbReference type="CTD" id="1018"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07860">
        <property type="entry name" value="STKc_CDK2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000144">
        <property type="entry name" value="Cyclin-dependent kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000599">
        <property type="entry name" value="Cyclin-dependent kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF462">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="8"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013915148.1"/>
        </source>
    </evidence>
    <sequence length="307" mass="35380" checksum="485CD4B8AC5501EE" modified="2020-10-07" version="1">MDTFQDVFQKVEKIGEGTYGVVYKARNKQTGQLVALKKIRLDSETEGVPSTAIREISLLKELKHPNIVRLLDVVHNQKKLYLVFEYLNQDLKKYMDSSRTGELPLNLIKSYLYQLLQGVSFCHSHRVIHRDLKPQNLLINEMGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRTLGTPTESLWPGVTNLPDYKGNFPKWPRKDMKVIIPNLDQDGRDLLLQLLMYDPNKRISAKAALNHQYFWQAPRDAKQCVVEQYGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXD1</accession>
    <name>A0A6I9XXD1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial S-adenosylmethionine carrier protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 25 member 26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SLC25A26</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mitochondrial S-adenosyl-L-methionine/S-adenosyl-L-homocysteine antiporter. Mediates the exchange of cytosolic S-adenosyl-L-methionine, the predominant methyl-group donor for macromolecule methylation processes, for mitochondrial S-adenosylhomocysteine(SAH), a by-product of methylation reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-adenosyl-L-homocysteine(out) + S-adenosyl-L-methionine(in) = S-adenosyl-L-homocysteine(in) + S-adenosyl-L-methionine(out)</text>
            <dbReference type="Rhea" id="RHEA:75479"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918827.1">
        <property type="nucleotide sequence ID" value="XM_014063352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXD1"/>
    <dbReference type="GeneID" id="106546476"/>
    <dbReference type="KEGG" id="tsr:106546476"/>
    <dbReference type="CTD" id="115286"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000199">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="131"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="221"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013918827.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="33852" checksum="C5BF4BF58632C02D" modified="2020-10-07" version="1">MDRGDFLISLGAGGLAGVCVDLTLFPLDTVKTRLQSPQGFKKAGGFRGIYAGVPSAAIGSFPNAAAFFVTYEYTKAVLRTADSPYFSPIIHMMAASFGEIEAAWLSDPEPALPTEGPRWLAISLQPRESFHRLLTKYYPFNVQFWLTNSFKSEIKLRKSIGSVFIINIVNFLEAITLSSGIPGLYRGYKSTVLREIPFSLVQFPVWESLKELWSWKQGHVVDSWQSAVCGAFAGGFAAAVTTPLDVAKTRIMLAKTGSRTASGNVLSALQGVWKTQGISGLFAGIVPRISSISLGGFIFLGMYDKIRSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXG1</accession>
    <name>A0A6I9XXG1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Occludin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">OCLN</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918862.1">
        <property type="nucleotide sequence ID" value="XM_014063387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXG1"/>
    <dbReference type="GeneID" id="106546504"/>
    <dbReference type="KEGG" id="tsr:106546504"/>
    <dbReference type="CTD" id="100506658"/>
    <dbReference type="OrthoDB" id="8867927at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF4">
        <property type="entry name" value="OCCLUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OCEL" evidence="5">
        <location>
            <begin position="124"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="139"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013918862.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="26391" checksum="830E659EA2D51130" modified="2020-10-07" version="1">MCKYLILIHNSSYDCAKVKNVSPTAVSPGAGYPDRVTSSMGYSVSDAPAHQIPKDNYTASPVPEVVVPLTKDLKQSQYVNPSRNNGSAKESQQKKKRGRTRRANNDNYDADYTTGGESCDELDENWDREYPSISSDQQRQAYKRDFDAGLQEYKQLQAELDEISKELSRLDKDLDDYIEGSEEYKAAADEYNRLKDIKSSADYKNRKAHCKMLKSKLSHIKRMVSDYDSRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXP3</accession>
    <name>A0A6I9XXP3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein-glutamine gamma-glutamyltransferase K-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921009.1">
        <property type="nucleotide sequence ID" value="XM_014065534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXP3"/>
    <dbReference type="GeneID" id="106548200"/>
    <dbReference type="KEGG" id="tsr:106548200"/>
    <dbReference type="OrthoDB" id="437511at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001143">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF49">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00868">
        <property type="entry name" value="Transglut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglutaminase N-terminal" evidence="2">
        <location>
            <begin position="80"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921009.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="27406" checksum="88B53B5B125947FF" modified="2020-10-07" version="1">MPVSDPRGDAGRWANVSYRVRQPSAPEPAPRRKKYWFHKFCRCCAGHRDDTDWTPAPGEVPGARRTDPVIREGMLVIQKIDLMSGRTGSNRRAHHTDEYEYDNLIIRRGQPFEMKLHFQQPYDSEDHRVCLEFLVGSSPQASKGTHILVPVGGSLPSPAWSAEMISSDDNTMSIRVCPSPRAVIGKYQFSVKTRSKAGEYAAPFDPKNEVYILFNPWCPEIRTSPTLMAFRKAVKTLLFLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXQ1</accession>
    <name>A0A6I9XXQ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Endonuclease 8-like 2</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">DNA glycosylase/AP lyase Neil2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">DNA-(apurinic or apyrimidinic site) lyase Neil2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Endonuclease VIII-like 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Nei-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26 27" type="primary">NEIL2</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26 27">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Binds EP300.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013918941.1">
        <property type="nucleotide sequence ID" value="XM_014063466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918942.1">
        <property type="nucleotide sequence ID" value="XM_014063467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXQ1"/>
    <dbReference type="GeneID" id="106546570"/>
    <dbReference type="KEGG" id="tsr:106546570"/>
    <dbReference type="CTD" id="252969"/>
    <dbReference type="OrthoDB" id="444592at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000010">
        <property type="entry name" value="endonuclease 8-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="FPG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="H2TH_FPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993:SF29">
        <property type="entry name" value="ENDONUCLEASE 8-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="N-terminal domain of MutM-like DNA repair proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Formamidopyrimidine-DNA glycosylase catalytic" evidence="24">
        <location>
            <begin position="2"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00056755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00081875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00082923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013918941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013918942.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="37572" checksum="42FB16BADDC046BB" modified="2020-10-07" version="1">MPEGPSVKRFQLLCSPYVGKVVSKVGGTTRQINPDDLKTLTLWDIQVHGKNLFLAFGTKEKLKPSHDSISLDLSEQRLEMSGSSSFLQGQKENEVEICNLECYQQEESASRLASDMDNNNGQSWKWLRFHFGLYGTIRANEFARANKANKRGDWKDPIPRLVLHFDAGNFLVFYNCRIQQCSSPSTDPATDILSPEFDQEQALEVLAKDTPVCYMLLNQSYFSGIGNIIKNEALYLAKIHPLSLGSSLESTDLKSLLDHIVQFSLAWLHNQLGRQRLPLQIYMKDKCPKGHEVKKETFGPPDGLKRLTWWCPKCQPRVPPEEMDRPLVQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY94</accession>
    <name>A0A6I9XY94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-amino-acid oxidase</fullName>
            <ecNumber evidence="5">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106539221</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013909440.1">
        <property type="nucleotide sequence ID" value="XM_014053965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY94"/>
    <dbReference type="GeneID" id="106539221"/>
    <dbReference type="KEGG" id="tsr:106539221"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF355">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Amine oxidase" evidence="12">
        <location>
            <begin position="200"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909440.1"/>
        </source>
    </evidence>
    <sequence length="473" mass="53543" checksum="EEBF178DD9CB2B4B" modified="2020-10-07" version="1">MGCQEMVVLTGYETVKEALVNQAKAFAERAVVPIFEDYAKGFGVIFAHGENWRQMRQFSIAALRDYGMGKRIVEDKISEECSVLIKTYATYEAILSLVPLLLAIWASVNHANEEEDDWMKCFEDPNYEELLNIARNGLEKTSNPKRIVIVGAGISGLTAAKLLKDAGHQVSIIQILHQMQNLNKLRDLFVDGIFVSMFLGFLNLFSFEEITGGFDQLPNSFCQEMSKIIYLNSRVEKIIRSSKYVKVFFHKDDEDDSSFLIADYVLVTATAKATRLIKFQPPLSLSKAYALRSLHYTSATKIALVCKDKFWEREGIRGGASLTDLPTRLVTYPSHDFPGGLGVLLVSYTLHDDADFFAPLSDDECLDVVMDDLSKIHNISKYYLESVCNRHVIQKWALDKFSMGAFTFPTPYQYNFFLKALFQNEGRVYFAGEHTTYPFGWIDSSMKSAIRAACSIHLNAHKSISQDLKDSVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYE8</accession>
    <name>A0A6I9XYE8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cdc42 effector protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CDC42EP5</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORG/CEP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921259.1">
        <property type="nucleotide sequence ID" value="XM_014065784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYE8"/>
    <dbReference type="GeneID" id="106548417"/>
    <dbReference type="KEGG" id="tsr:106548417"/>
    <dbReference type="CTD" id="148170"/>
    <dbReference type="OrthoDB" id="8898624at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031274">
        <property type="term" value="P:positive regulation of pseudopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029273">
        <property type="entry name" value="Cdc42_effect-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051296">
        <property type="entry name" value="Cdc42_Effector_BORG/CEP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344:SF15">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN BORG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14957">
        <property type="entry name" value="BORG_CEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="4">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013921259.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="24824" checksum="4549863F47A6DA8A" modified="2020-10-07" version="1">MPILKQSPVSHSKKRPRLDRAMISAPLGDFRHTMHIGRGGDAFGDTSFLSNHGGAKTNGLPHDVAVSPGSLGSPGDVSGCFELLSPPGSGNGVVQSPTTSTPDGSFMVLQSPEKADWNPPNGKGWDLQEKFSLDSPTLDHAESLLSFQLDLGPSILEDVLGVMDKDWDNIQGQDQEVLRDESLSPLGCSQQEDEKEQEEEEEEEEEEEEEEEGQGYSFEDEQDEEIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYU9</accession>
    <name>A0A6I9XYU9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">40S ribosomal protein S27-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106546963</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919452.1">
        <property type="nucleotide sequence ID" value="XM_014063977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYU9"/>
    <dbReference type="GeneID" id="106546963"/>
    <dbReference type="KEGG" id="tsr:106546963"/>
    <dbReference type="OrthoDB" id="5567124at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.100:FF:000001">
        <property type="entry name" value="40S ribosomal protein S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013919452.1"/>
        </source>
    </evidence>
    <sequence length="87" mass="9663" checksum="D2830A9EC0733659" modified="2020-10-07" version="1">MGEGGVFVQGHEEHLWLRQQRSINKNVLDTLKYLLGYLPAPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ90</accession>
    <name>A0A6I9XZ90_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Centrosome-associated protein 350-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106539536</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909810.1">
        <property type="nucleotide sequence ID" value="XM_014054335.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539536"/>
    <dbReference type="KEGG" id="tsr:106539536"/>
    <dbReference type="OrthoDB" id="306254at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028750">
        <property type="entry name" value="CEP350/CC187"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13958:SF3">
        <property type="entry name" value="CAP-GLY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13958">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN 350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="3">
        <location>
            <begin position="1179"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="466"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="773"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1060"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1124"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1245"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="419"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="735"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="579"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="627"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="653"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="780"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="806"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1060"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1124"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1135"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1261"/>
            <end position="1277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013909810.1"/>
        </source>
    </evidence>
    <sequence length="1775" mass="202049" checksum="5C00767FF1968371" modified="2020-10-07" version="1">MCSKKASEFPLHNPKFSQNRNSIRDLTTAWNSVSQAKATLQHIENKLEADLISTAGLDTVMEKKPSISTTRKISRKGCRSSSQNKESSSRRPLRATTLDSNVSKNGHVELHQPLSSFRDSRWSPSYKTSCQLEARRLKYKETGNKAERSGMIYDKGESDLHGRDLKRSSSTDETIVRYLNDRPAINALQNSDVVNHTKLEEKSEESRGDEYTKTSSSSIIQNELKEFPLVESSASSASTSSAHRLDILKQHQHDDKLEKLKEQIRKQWEFSEELIGRGYNPEHSDHPITITAPENIVTPKIRKVATAPMAPSYKGFNPSEAKIRTPDGKVWCEEEFLNLNRDLCQDFEFQLAEEDLTAIKTPTERKEKKGHKTVRKTQKIAQLSSPKSGAFYCIFCYVNFEKALEMRLISDCAMLIFCNAFLKAEIKRLQEAYKAARKERHLILKQQEEIKRIRQTTMKLQEKLKFAGENKEHRSEEDKKQTHSSSPQLSDAEAYSPSSISSSETSSIMQKLKKMRRRMDEKFLTKREQKLMQRRQYAEELLQWKQRLDAEEAEIRQIEKQAQAAWEKELPRIKANKKGAGDQKSDPKDAASEEDSPMPPCSHLNSKSSVPEELSNLVVESIPSKVSEIKDEPGSLDHTITEEIVYTEEPKSLVSTGKPSPCKSSASLSKQESNKQTQKSLGKQLSSTKSQDISYSWSDESLSMTQSETTSDQSDIESRIWALKDQVRKKKSVVYRLKKEQKKRLKEKLKAQEASLIKQLETYDEFIKKTEEELNRDLETTPTAKPQIKTPTSVPEKPKIKPPPLHRSETSKSWKSLTDSERSRGSLESIAEHIDAVSHFEQSVSINIMKFTEEEIHEGKQSHTASRVLKTRRKSIAESDDLDSVLSSSILKDLENTSKISPISMNKPEDAVSYDSPAIVQETAESKELEVEIVSQAYEISNAPVFDLNQNNSSPLKKEVPPETELLEKDQFVLECKSVQEIKEMPSETFEDDENITSVKSTDRLGKDTLKDDSLVSEQISICEEQSYSSDFEESSLGTDISKSEIVLTEYQRDIFQEPTLSFRQDAGSQAENSLYTRPPRGESPGFGSDDEISECLSEKSLSVVGSIQSERLLELQSPTEFVKNKECGDVEKEPPDDDDDDDSSWASSGEQEDILLGFNIGDRVLVSNLHLGTLKFKGRTNFDTGFWAGVELDKPDGYNNGSFNDIQYFDCKEKHGIFAPPQNLSLFSESVGNNLDSNENSFFKRELDNQPKTNQGGLDGLEKEKNERNSNEKSPKDSSALKSSIGAGDYINFNKSRSCVVVISPDDEFDQETSDFDSLKSSKNDNGMLPPAISNIHKENSAIEVGGIVDNSFPEKWKQQSVWQEQTENRFSSSNLEKSATPLLDLLTKERSQLEAQLKVPSQEKEDVLDRQERVGLLADSLLQGFVKDTVNQFQQIKKARNEKIHFSNQELGAVQEKNLAFNEQQRFLVASELDDGEEVSSPDMCPRPESPVFGASGQEELAKRLAELELSREYLNVLGDDQDWSDEDFGLSSHQLPENQAEESEVLPKIEVPKVSQKPCEEPLDALYVAEEVEKLVHAATEKLWKCKELGHDLRSLSSHRDMNESTQDDDIETINKQISKQLVFDLTREIFEEIFSEDPNLNRPLWMKPRRIISGYYRRVRDPRNLEEIKVFITAEVLKLLNLRNESNNKTDWQKTMKFGRKKKDRVDQILVQELYEEEAQWVNYDEDELYVKMQLADGIFEALIRDTIDVLNRIYEKKKLLIVERQNPPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0V4</accession>
    <name>A0A6I9Y0V4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SH3TC2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920482.1">
        <property type="nucleotide sequence ID" value="XM_014065007.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547756"/>
    <dbReference type="KEGG" id="tsr:106547756"/>
    <dbReference type="CTD" id="79628"/>
    <dbReference type="OrthoDB" id="9321902at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901184">
        <property type="term" value="P:regulation of ERBB signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033157">
        <property type="term" value="P:regulation of intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042772">
        <property type="entry name" value="SH3TC1/SH3TC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22647:SF2">
        <property type="entry name" value="SH3 DOMAIN AND TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22647">
        <property type="entry name" value="SH3 DOMAIN AND TETRATRICOPEPTIDE REPEATS CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="207"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="297"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013920482.1"/>
        </source>
    </evidence>
    <sequence length="1324" mass="150607" checksum="AB40FD0E73A7D7CF" modified="2020-10-07" version="1">MSPDERPGAESKSSSIQSVLLASWSSLQHCQAETPERCFPEKKMASGNPKELDPGSHPAQIGEGTLETESVSLAVGEGFPNAISLSFSVKSHSAQDLNLQLQEVARKKLWALENDDKDVCAVFKELSARLACIQAQENRFLLSFRTVEEIWKFSTYLALGYVGCCLEHLLFSPEYWLNCAQVDDTEITVTVDEDCMTTMYLGLLLQEGNFFARATLTVPAEEEEEDLKLLKNELVHVKDIGHETKWEGTSLLTDQRGLIHVTTIEPLPHPFYQWFLKNYPISCGISKEISGPDSLQIGQGRCTATEDHRGKSWDELSYCKGDILEVIGFLIPGLRWFIGKSTTSGIIGFVQTKSVQLESCESMRNSPVFFSEEEISAFFQAPCNGNEVYYSNLLNEWAQTDITSVYRLDGFEPIVMYPQRPPDAAVHGHKNGWMFENWGKQSYNSLCTQTDSEKSTPVAESSPSTLEHLLLPEPDDLDDPKFFIDLNAGHMEDCDVFDPILTFLNQDSYVPHFQSLYDLSFSFLSSTFYGFTSEEELVVYLETSRNWAKRGHLGWAHIRICYLLGRLCVRKAKFSQARVYFEEAMNAMDKGFEDLPLLTSLHANLAAIYLKQKMKQKFLPMIGKGMTLLACLSGHCFSSETELEVVMYILREAITVSNTTMEIRSCFLIVRLFLQLGKYDEVLPFAERLQFLNAQLTSQVTSTYPLDTMPFLAYLYEKKYVPHVALAAARMYSPSNIKSGFTPIWRAGFILANTSKLLKAQEKSHNSISALACFYLHCGLSFSHEAGTVSTEKALCALLSKLYLKYGMLEKAVHYSKQAVALSKVMGEEEAFEASLSLGWLFTLNNQPDKTSEIMQYLLCSLRQTDSVTQDGVVHNLLAIALKREGQVQKAAENYFWALKKAQENGNQRNQAIALANFGCLITSLGAYYLGEYYFLQAIQLYFELQDGDDTQMELVHILLWLAQSKLERCKAEEGIIFYEMALAIALKTQNVISQLHVTESLCHFYSKVHPDTRTCILYHEHQVLLLRQLQNREKEGHLLQDLSQLYQSLHTPRSLKQALDCTKQSLRIFIDLEEIVKTAQAWLQAGRLYYQMQENELVEMYLQASIETALKPKELHLAMGLYEEAGDVFFNGIRNRHQAVEYYRGGAIPLARKLRDPQTELRVFNKLAELQIGLHNYENALEFATLAVRLSVDVGDHLQELVAFHRLATVYYLLQMYEMAEDCYLRTLSLHSPELQGVQEAIYYSRVYYRLGDLTLHKLKDEQDAASYFFLALAATTEFDDRNWQGKIQARLAQIFTNSQTNKSPRGWTTYRARWLSEGRHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y146</accession>
    <name>A0A6I9Y146_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Lysosomal alpha-glucosidase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106544831</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916698.1">
        <property type="nucleotide sequence ID" value="XM_014061223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y146"/>
    <dbReference type="GeneID" id="106544831"/>
    <dbReference type="KEGG" id="tsr:106544831"/>
    <dbReference type="OrthoDB" id="5839090at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000072">
        <property type="entry name" value="lysosomal alpha-glucosidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1760:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF104">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="12">
        <location>
            <begin position="70"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013916698.1"/>
        </source>
    </evidence>
    <sequence length="722" mass="81261" checksum="3E883AE92782EE6C" modified="2020-10-07" version="1">MKSYQKLSTDIPGITEAGEEDPDGKPLCHQKLSAWWLGGGLLMVAALLSGVTVWVLGQIWWGQHVVPCLSKCTSVPENRRFDCYPEKSMIVTQELCEGRGCCFVDGETPLDRINGIPWCFYPPDFPSYTLGATNETELGLVAFLTRETKTYYPKDVEKLQLSVDFETDSRLRVKITDATNPRYEVPLDVPQATKKAENPLYSVELCKEPFGLVVKRRLSGTVLLNTTVAPLFFADQFLQISTVLPSTFLYGLGEHQSNFLHNMEWKTLPLWARDVPPTESYNLYGVHPFYLLMEKAGAAHGVFLLNSNLMEVALQPAPALTWRTTGGILDFYIFLGPDPNLVVQQYQQVIGFPAMPPFWGLGFHLCRWGYGTSNKTWETVKAMRNFQIPQDAQWNDIDYMNSTRDFAVDSDKFASLPQMVEDLHKHGQHYVLILDPGISSTQRPGSYWPYDEGLRRGVFINNTGGKPLIGKVWPGFTAYPDFSNPDTDEWWLENLKHLHTQVPFDGLWIDMNEPSNFIDGSTEGCSKGDLDNPPYTPGILGGSISAKTICASAKQRVSVHYNLHNLYGLMEAKATSRALVELRGKRPFVISRSTFPSQGRYSGHWLGDNRSEWKDMFWSIPGLLSFSLFGVPLVGADICGFSSSTSEELCVRWTQLGAFYPFSRNHNTQLEKAQDPTAFSPAARTAMKAALELRYTLLPLLYSLFHRAHLQGDTVARPLFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1F2</accession>
    <name>A0A6I9Y1F2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">E3 ubiquitin ligase TRAF3IP2</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Adapter protein CIKS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Connection to IKK and SAPK/JNK</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">E3 ubiquitin-protein ligase CIKS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nuclear factor NF-kappa-B activator 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">TRAF3-interacting protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TRAF3IP2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with IKBKG/NF-kappa B essential modulator, with CHUK/IKK-alpha and with IKBKB/IKK-beta. Interacts with TRAF6; this interaction is direct. Interacts with IL17RA and IL17RC. Interacts with IL17RB.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013910610.1">
        <property type="nucleotide sequence ID" value="XM_014055135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1F2"/>
    <dbReference type="GeneID" id="106540115"/>
    <dbReference type="KEGG" id="tsr:106540115"/>
    <dbReference type="CTD" id="10758"/>
    <dbReference type="OrthoDB" id="6021171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006959">
        <property type="term" value="P:humoral immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097400">
        <property type="term" value="P:interleukin-17-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038173">
        <property type="term" value="P:interleukin-17A-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11530:FF:000007">
        <property type="entry name" value="adapter protein CIKS isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053047">
        <property type="entry name" value="E3_ubiq_ligase_TRAF3IP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34257">
        <property type="entry name" value="ADAPTER PROTEIN CIKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34257:SF2">
        <property type="entry name" value="E3 UBIQUITIN LIGASE TRAF3IP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SEFIR" evidence="14">
        <location>
            <begin position="386"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="128"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910610.1"/>
        </source>
    </evidence>
    <sequence length="555" mass="63440" checksum="88832BF309CBA50E" modified="2020-10-07" version="1">MTSLQGATMSRSIPVEVDETIDWSPFPEHSLKEASNPSVEVINNSMPMGSYAPQTSHPIYGQLGFYKHLDHVNYNNSILEAYGHSLPEDTYDSRRGQFPGPSSNSTDRSECNHLPQHKTNQLLRSQPSADSLDIGHNSSNLQRSSSEISQHSFGENQENVKPNWQHDNRVPVDLRTEDAGYDSQPQDPLGIGQPELPLSFTSVLNIQDLPRSLRSREYPQMDSQSYPLFPQMPHLYNSPQAQWHPRYYNQNMYRQNPYGQTPYQHFARSPQVQLPLPGPCARVTRPAQQIIPHYSSPHGPKCNEERLPQGESSSEGLQRFHDQFQNQMLENNAPFKIHGAHEGIERFPFDSMAQSGSMTTSGAIPRPLTNSAARGTLRTSNLPEELRKVFITYSVDAAVEVMKFVNFLFVNGFQTAIDIFEDTIRGIDIIKWMERYLSDKTVMIIIAISPKYKQDVERAESQLDKDEHGLHTKYIHRMMQIEFIQQGSMNFRFIPVLFPNAKKEHVPTWLQNTHVYHWPRNKKNILLRLLREEEYIAPPIGPLPTLQVVPLGAHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1Q2</accession>
    <name>A0A6I9Y1Q2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proline-rich receptor-like protein kinase PERK2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106545013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916928.1">
        <property type="nucleotide sequence ID" value="XM_014061453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1Q2"/>
    <dbReference type="GeneID" id="106545013"/>
    <dbReference type="KEGG" id="tsr:106545013"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916928.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="37304" checksum="720AAB9CC50B77EA" modified="2020-10-07" version="1">MKVNRMLQPMPKPENRTVVLVNSPRPTTPLQRPITPVLSEPLTPAIVPVPHTGPVLPGPLQPTTLPASVSLPVSIPMPPSPVTMSVPVPPPTKPQTPTVVPTLSQNNPAATLLQSQASVPQVLPLAMTAAQLVPNTPRLLLSPPPVSPTTTTASSLKAAPAPVPMGQQPPVSLSATPVRQQPPINTLSIASVQQQPPISTLGISSMAQQPPTNSQSINTLSISPINTLGVSPVVGQQPPPTNTLGPGPAGTTAQQSTSSIGGMVKPMNIHSAIHTLPGYNFAAAAGGVQQRLLLSPDMQARLPSGEVVSIGQLASLANRPLQGAPGSKPLTFQIQGNKLTLMGTQVRQVTMPQPAQQLPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y263</accession>
    <name>A0A6I9Y263_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Hemojuvelin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HFE2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910880.1">
        <property type="nucleotide sequence ID" value="XM_014055405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y263"/>
    <dbReference type="GeneID" id="106540328"/>
    <dbReference type="KEGG" id="tsr:106540328"/>
    <dbReference type="CTD" id="148738"/>
    <dbReference type="OrthoDB" id="10013795at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.10:FF:000001">
        <property type="entry name" value="Repulsive guidance molecule BMP co-receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF3">
        <property type="entry name" value="HEMOJUVELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="12">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013910880.1"/>
        </source>
    </evidence>
    <sequence length="307" mass="33456" checksum="D3F73EA5308D6415" modified="2020-10-07" version="1">MAQHNCSKEGPSPGPRPPQPRRAGSPDLCNYEKAFAHKRGRPPTYQHCASFGDPHVRTFGDDFHTCRAEGSWPLLDNHYLYAQATNSPVAKGSDATAMSKITIIFKNMKECVDQKVFQAEVENLPAAFVDGSVNGGERPGGDSLSILERAPGQHVEIRAAYIGTTIAVRRAGKQLSFSIRVAEEVASSFTEKQDLQLCVGGCPASQRISRHTGCCGHGTIATEKARRLCKEKLPVEDVYFHSCVFDVVASGDSNFTLAARGALEDARIFHPDSQKLHLFQQDAARQVATSSFLCLTLAAILLLAWEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2I5</accession>
    <name>A0A6I9Y2I5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein 362</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ZNF362</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917278.1">
        <property type="nucleotide sequence ID" value="XM_014061803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2I5"/>
    <dbReference type="GeneID" id="106545290"/>
    <dbReference type="KEGG" id="tsr:106545290"/>
    <dbReference type="CTD" id="149076"/>
    <dbReference type="OrthoDB" id="5305647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000377">
        <property type="entry name" value="zinc finger protein 362 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000549">
        <property type="entry name" value="zinc finger protein 384 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="101"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013917278.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="32066" checksum="D99C57192D1A4636" modified="2020-10-07" version="1">MAEPRFNNTYFWPPPPTMPSQLDNLVLINKIKEQLMAEKIRPHHLPPTSVSSQQPLLVPPSPAESSPSIMSIPKLQQVPGLHPQAVPQPDVALHARPATSTVTGLGLGSRTPAVSTSESSTGTGTSTASTPTSSSQSHLIASSPTLISGITSPPLLDSIKTIQGHSLLGAPKSERGRKKIKAENPSGPPVLVVPYPILASGDIGKEGKTYRCKVCPLTFFTKSEMQIHSKSHTEAKPHKCPHCSKSFANASYLAQHLRIHLGVKPYHCSYCEKSFRQLSHLQQHTRIHTGDRPYKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2Q1</accession>
    <name>A0A6I9Y2Q1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DDB1- and CUL4-associated factor 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106540513</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911115.1">
        <property type="nucleotide sequence ID" value="XM_014055640.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540513"/>
    <dbReference type="KEGG" id="tsr:106540513"/>
    <dbReference type="OrthoDB" id="24670at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013911115.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="20322" checksum="050514D23818DF8F" modified="2020-10-07" version="1">MYVFPPLSSQTMPGTSSIDTTCTIWGLETGQVLGRVNLVSGHVKTQLIAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEHSTIIYEDPQHHPLLRLCWNKQXLIFYIPLLSQNNVADDHQALIWDIQQMPRAIEDPILAYTAEGEINNVQWASTQPDWIAICYNNCLEILRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3I1</accession>
    <name>A0A6I9Y3I1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Insulin-like growth factor-binding protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">IGFBP7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917918.1">
        <property type="nucleotide sequence ID" value="XM_014062443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3I1"/>
    <dbReference type="GeneID" id="106545785"/>
    <dbReference type="KEGG" id="tsr:106545785"/>
    <dbReference type="CTD" id="3490"/>
    <dbReference type="OrthoDB" id="10012075at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005520">
        <property type="term" value="F:insulin-like growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000763">
        <property type="entry name" value="Insulin-like growth factor binding protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011390">
        <property type="entry name" value="IGFBP_rP_mac25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14186">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14186:SF19">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018239">
        <property type="entry name" value="IGFBP_rP_mac25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026681938" evidence="7">
        <location>
            <begin position="22"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="9">
        <location>
            <begin position="28"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="87"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="154"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013917918.1"/>
        </source>
    </evidence>
    <sequence length="275" mass="29005" checksum="A05B5EFC66028821" modified="2020-10-07" version="1">MMRAIWCAPSCLLLLLGCAASLEEPGAAAGGCSPCVPDECPPLPAWGCPLGKVRDNCGCCWRCARGEGEACGNGVSGGVTVVWGRCATGLECRRRTKNRGATAVCVCKNRYPVCGSDGLTYPSTCQLRAASLRAKSRGERPVAQRSKGPCEQGPSIVTPPKDIWNVSGAQVYLSCEVIGVPTPILTWNKVTRGQYSVQRVELLPGDRDNLAVQTRGGPEKHEVTGWVLISPLSNDDAGEYECHASNSKGEATASGKITVVNSLNEIPAKGESTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y569</accession>
    <name>A0A6I9Y569_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">T-cell leukemia translocation-altered gene protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TCTA</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TCTA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912885.1">
        <property type="nucleotide sequence ID" value="XM_014057410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y569"/>
    <dbReference type="GeneID" id="106541859"/>
    <dbReference type="KEGG" id="tsr:106541859"/>
    <dbReference type="CTD" id="6988"/>
    <dbReference type="OrthoDB" id="9529463at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072675">
        <property type="term" value="P:osteoclast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016560">
        <property type="entry name" value="TCTA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32267">
        <property type="entry name" value="T-CELL LEUKEMIA TRANSLOCATION-ALTERED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32267:SF2">
        <property type="entry name" value="T-CELL LEUKEMIA TRANSLOCATION-ALTERED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15128">
        <property type="entry name" value="T_cell_tran_alt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009935">
        <property type="entry name" value="TCTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013912885.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12316" checksum="6D8464F0599C8024" modified="2020-10-07" version="1">MAAEAEAGADPASAEWEAPTRVLRALTWLGQEFLSDWEAQDLRAALFQLLLLWLLVSLLGIQVAWRVYGNTVTSLYYRQGAGGQNGETPDGSSHFSMWESTSNTNTKTHRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5P2</accession>
    <name>A0A6I9Y5P2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NEDD8-conjugating enzyme UBC12</fullName>
            <ecNumber evidence="7">2.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">NEDD8 carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">NEDD8-conjugating enzyme Ubc12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">UBE2M</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.34" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013913320.1">
        <property type="nucleotide sequence ID" value="XM_014057845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5P2"/>
    <dbReference type="GeneID" id="106542185"/>
    <dbReference type="KEGG" id="tsr:106542185"/>
    <dbReference type="CTD" id="9040"/>
    <dbReference type="OrthoDB" id="10249039at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061654">
        <property type="term" value="F:NEDD8 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000239">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="16">
        <location>
            <begin position="29"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913320.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20943" checksum="8E05E3B4FC22B203" modified="2020-10-07" version="1">MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCEIDFSDQDDLLNFKLVICPDEGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5V2</accession>
    <name>A0A6I9Y5V2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Coiled-coil domain-containing protein 178 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6" type="primary">CCDC178</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920115.1">
        <property type="nucleotide sequence ID" value="XM_014064640.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920116.1">
        <property type="nucleotide sequence ID" value="XM_014064641.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920117.1">
        <property type="nucleotide sequence ID" value="XM_014064642.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920118.1">
        <property type="nucleotide sequence ID" value="XM_014064643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5V2"/>
    <dbReference type="GeneID" id="106547458"/>
    <dbReference type="KEGG" id="tsr:106547458"/>
    <dbReference type="CTD" id="374864"/>
    <dbReference type="OrthoDB" id="10010556at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038826">
        <property type="entry name" value="CCDC178"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35088">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35088:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="275"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="363"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013920117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013920118.1"/>
        </source>
    </evidence>
    <sequence length="809" mass="95895" checksum="031198FB1F38D592" modified="2020-10-07" version="1">MTDAQLFSPCSKENCKETEDINENISIGIPNKQRSCAAVNTPASCVNKVINQVQDLEVKLEESFLQHEVLYNLEKLPRDLDAKTVKETEVKAEQSLHWKDIQSLKQKTSAVVAEVTELISRLENERKDAEEALEFEKQQRKKLYLNSDKLSFWRLQQLPKAVQKEWEKCLQDIIELQWHFEEKNEQLHDALNQLTKIEGANSVIIKQINFMKKYSPLLEEKLCHEDESIKEVKILFDEAKAQYDTVHQKYLEVQMFHEEVKEECEIKRQYMFQQIEADETTIKQLRDELKETDRLYADINNKIHQLREEILENEKRLEELIKQEADAKADLNSWRDKERRLRLKNSIQESENKQLLDEFLKEMKSMENSKSNKEYDLQKLKDKLAHDLQEFTSLQNEIQDLKNENAGFMYKFRDTARRKMGYQSEIQVLQKNIRRLEEHLKKVNKELYAAELAYDDAKTKLEDLSEGIAKEKSRFKNLEENIKKQIRDEISAWKLTQKRIKALEAELERKQREHKKLKEKIRSKLEKYEQLSAEQVEILEKNTNLHKELIEKIAELTEKIKELDDKEGNVKNQLENKKKYAQNLLDDKKEKYFGISSQFTKVNKEIVHLQKEIAKLSALRKGQENQVENIEKSVITLREKYTKIKSKEENIRRLVDFLHARLDTVRKKTKTDNRILDQLLWTRQNDLKIKKISLDDILDENLRLAREYQMLQICYLNDKNQLMDFYDHKDRAKAVLRDQQELSQLQKKLHRLVVEYFKLQALYNQAKLGKLQGASHESVQKILAVKGRLSETVQNTNTFLKSLTDGSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6R6</accession>
    <name>A0A6I9Y6R6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine beta-lactamase-like protein LACTB, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LACTB</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914230.1">
        <property type="nucleotide sequence ID" value="XM_014058755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6R6"/>
    <dbReference type="GeneID" id="106542897"/>
    <dbReference type="KEGG" id="tsr:106542897"/>
    <dbReference type="CTD" id="114294"/>
    <dbReference type="OrthoDB" id="5946976at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052794">
        <property type="entry name" value="Mito_Ser_Protease_LACTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46520">
        <property type="entry name" value="SERINE BETA-LACTAMASE-LIKE PROTEIN LACTB, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46520:SF1">
        <property type="entry name" value="SERINE BETA-LACTAMASE-LIKE PROTEIN LACTB, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026826254" evidence="2">
        <location>
            <begin position="31"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="3">
        <location>
            <begin position="55"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="3">
        <location>
            <begin position="241"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914230.1"/>
        </source>
    </evidence>
    <sequence length="492" mass="54442" checksum="1577BCFB0955F31A" modified="2020-10-07" version="1">FHGGGGGGGRWPLWRLGVGLGLAATAVVVAGPERDSEERPPREAGFQRAVVRSRDLLQRLKDQVGVPGVVVGVSVDGKEVWSEGIGYADLENRVLCSPETVLRIASISKSLTMVAVAKLWEEGKLDLDTAVQKYVPEFPEKEYEGEKVTITTRLLVSHLSGIRHYEKDISKVKEEKEKANRKASRTAVSAAKEKEDGEPGKKRDSAKGRTEEEGKPAKSGKKKREFEHEEYYLKDKFEHVVDSLNIFQNDPLFFKPGSQFLYSTHGFTLLSAVVERASDQKFTDYMLKIFSDLGMGSTGLDENEPLIYNRARYYIHNKKGHLVNAPYVDNSYKWAGGGFVSTVGDLLKFGNAMLYSYQLGRVKNPAAGLLPGYLKPDTIATMWTTVPSTRASQESDYAYGMAWVVVEKKQEYGHCKELRHYTFHTGAAVGASSVLLILPEEGTASGSCLVPPQGVVVTIICNMQSVHLDGIALKIAKEFEKDRLEKKCGRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6V0</accession>
    <name>A0A6I9Y6V0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Voltage-dependent L-type calcium channel subunit alpha-1D-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106548223</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921038.1">
        <property type="nucleotide sequence ID" value="XM_014065563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6V0"/>
    <dbReference type="GeneID" id="106548223"/>
    <dbReference type="KEGG" id="tsr:106548223"/>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF2">
        <property type="entry name" value="VOLTAGE-DEPENDENT L-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 14" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3 14" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7 14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="150"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="338"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="351"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="13">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013921038.1"/>
        </source>
    </evidence>
    <sequence length="449" mass="50797" checksum="7D9040F3A2DC42BE" modified="2020-10-07" version="1">MSKSECNGGPLGSPEAPPQSAAEAFVEASGQAIAWTLTSGGSDTLGSTGSIVQKKRQQSKHKPQGSNVVHRSPRALFCLRLNNPIRRAAISIVEWKPFDILILMTIFANCVALGVYIPFPEDDSNVSNHNLKLEMVIACLSEAKFRNTCLHIVLNSIMKAMVPLLHIALLVLFVIIIYAIIGLELFIGRMHKTCFIIDSDLEAEEDPSPCAFSGHGRECTVNNSECRGKWDGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWVSYGLLGKGTERSSVHTSLYQLNLTSSIRREFSKEREKAKARGDFQKLREKQQMEEDLTGYLDWIMQAEDIEPDEDKDETSEKHTSSLPASESTSVNTENVEDEEHLQSHCYDVCLRKFSKTKFGRHVQRINRLFRKKCRLAVKSVTFYWIVLLLVFLNTLTIASEHYRQPYWLTQIQGETYKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y807</accession>
    <name>A0A6I9Y807_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 883-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106544371</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916095.1">
        <property type="nucleotide sequence ID" value="XM_014060620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y807"/>
    <dbReference type="GeneID" id="106544371"/>
    <dbReference type="KEGG" id="tsr:106544371"/>
    <dbReference type="OrthoDB" id="8999342at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000056">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003000">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002604">
        <property type="entry name" value="Zinc finger protein 715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000516">
        <property type="entry name" value="zinc finger protein 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="75"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="500"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="528"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="584"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013916095.1"/>
        </source>
    </evidence>
    <sequence length="625" mass="71009" checksum="DB94AE4885BF5283" modified="2020-10-07" version="1">MELPQVAFEEVAVHFTEQEWALLNVGEKVLYRDVMKENFEHVMSLGILLALKESKIAPCLLLVTPYSSSVTFRQVSFEEVAMHFSEQEWALLNVNERALYKDVMQENYKHILSLGEISQPGSPINTTSFGRLVDHLQATESPNSDLRYLREECQEEKQIVQGSFDEPVPLRRSTQAAEGPPPEVGERKDSCPKCGKTFCHKSALTAHLRIHTGEKPYHCQECGKSFNQSSALTQHQRIHTGEKPYACLSCGKRFSQSSALTQHQRIHTGERAYTCLDCRKSFIYQSALIRHRRIHTGEKCYTCPDCGKGFNQSSNLITHRKIHKGMEGNETAYGNDKKSPELENRVHLEPHSNTYMTFQQDCPQSSPLEKCLLDWKISKRNPSDEAQAAPVPSTGSLISQKDTVRKVGIPRVKRPLICHVCGKNFRYSSHLVNHQRIHTGEKPYHCTDCGKNFIQNSALKRHQRSHRGDRPYRCPDCGKSFSRNAHLAKHQGIHTGLKPYECLDCGKKFSVKMNLVIHQRIHTGEKPYMCLECGKSFSQRAHFIIHRRIHTGEKPYECPDCGRAFRVSSHLVTHQKIHMAKTSFICPDCGKSFNGGKQFIQHKRCHATGENSPLPNSGVTIKSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y839</accession>
    <name>A0A6I9Y839_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106544497</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013916255.1">
        <property type="nucleotide sequence ID" value="XM_014060780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y839"/>
    <dbReference type="GeneID" id="106544497"/>
    <dbReference type="KEGG" id="tsr:106544497"/>
    <dbReference type="OrthoDB" id="10016527at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051234">
        <property type="entry name" value="TAO_STE20_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TAO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167:SF10">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TAO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="225"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="114"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013916255.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="37455" checksum="23A3C944FC68B3BA" modified="2020-10-07" version="1">MTQYVRASQSYSYVKLERYFKFLGDPINVRNQPKAKQGGWRRATSTSGLSPSFYPFLQDHVFIRDEAGQGERRPELRPTHSVQNQALHYRNREAFATIKSASLVTRQIHEHEQENELREQMSGYKRMRRQHQKQLIALENKLKAEMDEHRLKLQKEVETHANNSSIELEKLAKKQVAVIEKEAKTAAADEKKFQQQILAQQKRDLTNFLESQKKQYKICKEKIKEEMNEDHSTPKKEKQERISKHKENLQHTQAEEEAHLLSQQRLYYDKNCRFFKRKTMIRRHELEQQNIREVSAFSLCPPPPPPPKKTTSKGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y995</accession>
    <name>A0A6I9Y995_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b-c1 complex subunit 1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex III subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Core protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquinol-cytochrome-c reductase complex core protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106546049</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with UQCC6. Interacts with STMP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase M16 family. UQCRC1/QCR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918285.1">
        <property type="nucleotide sequence ID" value="XM_014062810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y995"/>
    <dbReference type="GeneID" id="106546049"/>
    <dbReference type="KEGG" id="tsr:106546049"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000016">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF116">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="17">
        <location>
            <begin position="61"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="18">
        <location>
            <begin position="212"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013918285.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="53401" checksum="1B6660730B6AA97E" modified="2020-10-07" version="1">MAAGSVYRALGCVASRALLRGSCAPISLQGLVKRGSTATYANVLANLPETEVTTLDNGLQIASEKSNHPTCTVGVWINIGSRYETEKNNGVGNFMEHLAFKGTGKYPHAQFEKEVEGIGAHLDSYYSREKTAFYMKILPKDLPKAIEILADITQNCSLEDSQVEKERNVILQEMKESDSCLSAVLFNYLHASAYQGTSLAQTAEGTTANIKKLTRSDIAEYMGTHLKAPRMVLVAAGDVGHKELVDLAQQHFGNVPSNYKGGNIPVLSKSRFSGSQVTVRDDELPLAHVAFAVEGPGWGSPDNLVLLLINSIIGRYDVTFGGSKNLYSKMAADFAEHDLCQSFQTFNLSYSDTGLFGFYFVSGRFNILHSSDWGRYEWMRICTDLPEHDLERGKKVLRRNLVAQLDGTTPMCESIATQLLSYGRRIPLAEWDARISEIDTKTVQDVCSKYVYDKCPVVAGVGPVEQLLDYNYNRTWTYLNRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9J5</accession>
    <name>A0A6I9Y9J5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CDK5 regulatory subunit-associated protein 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106546438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918785.1">
        <property type="nucleotide sequence ID" value="XM_014063310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9J5"/>
    <dbReference type="GeneID" id="106546438"/>
    <dbReference type="KEGG" id="tsr:106546438"/>
    <dbReference type="OrthoDB" id="10587783at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097431">
        <property type="term" value="C:mitotic spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000242">
        <property type="term" value="C:pericentriolar material"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046600">
        <property type="term" value="P:negative regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042791">
        <property type="entry name" value="CDK5RAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46930">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46930:SF1">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013918785.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16072" checksum="AD408109C4A6ACEF" modified="2020-10-07" version="1">MLGVLPPTIFFSLEDTLISWSNQTSYMSICLRDQDYRLDCPVDQLSMEELKKKFVEVLGVVKEMHWVIQELKKKPFDFSTSDPSGNEKQKSLDTSELLERTEESQTLLGASDEEASAPSCDGPETLSEVGVDWPGGLFFKGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAM4</accession>
    <name>A0A6I9YAM4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FERM domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FRMD5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920680.1">
        <property type="nucleotide sequence ID" value="XM_014065205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAM4"/>
    <dbReference type="GeneID" id="106547929"/>
    <dbReference type="KEGG" id="tsr:106547929"/>
    <dbReference type="CTD" id="84978"/>
    <dbReference type="OrthoDB" id="6266673at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17102">
        <property type="entry name" value="FERM_F1_FRMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000458">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000006">
        <property type="entry name" value="FERM domain-containing protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF5">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="2">
        <location>
            <begin position="11"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920680.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="30016" checksum="430384419266B70D" modified="2020-10-07" version="1">MERYDSVCRFRVIHHQYTDGTQLCTPQLGRNQRDAKGQFLFDLLCHHLNLLEKDYFGIRFVDPDKQRHWLEFTKSIVKQMRSQPPFTMCFRVKFYPTDPAALKEEITRYLVFLQIKRDLYHGRLLCKTSDAALLAAYILQAEIGDYDPGKHPEGYSSKFQFFPKHSEKLERKIAEIHKIELSGQTPATAELNFLRKAQTLETYGVDPHPCKVTEQSKPPSTMKKSEEGYASQERNQKDGNTKKSSNDFIIIEENYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAM6</accession>
    <name>A0A6I9YAM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC106547933 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106547933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920690.1">
        <property type="nucleotide sequence ID" value="XM_014065215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAM6"/>
    <dbReference type="GeneID" id="106547933"/>
    <dbReference type="KEGG" id="tsr:106547933"/>
    <dbReference type="OrthoDB" id="382863at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053347">
        <property type="entry name" value="Axonemal_MT_stabilizer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37404">
        <property type="entry name" value="HCG1796489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37404:SF1">
        <property type="entry name" value="HCG1796489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013920690.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="37034" checksum="A07E10A724D3F05E" modified="2020-10-07" version="1">MAGTFSAFPFGWEVDSGALVPPEVRRQDWRLTSSGVGLNYSPLLPFPPPYALSTIQEPLPPASQKIWRDEVIPRLATTAQLAHCMKTQRELLAQPRYSVAASYQDVHYNKDLREKIKLREWRFPLTMGNQKSEIQANFRGWPNLPHDPTFHAGPQPFKLAAHHTDGASKAVVATTQNNNLAGRPFYICDKTVFQLNEPYLSTTAQDFRIFTQKELQGCTRKQILNLDTYPLIRVPGPMRDLQTFPKATRVPHLILNPAVPHRGLFSLAQETYQLPNDHKRTWDRFCPVQQPPKVSCRVPVVEIMCVPHMYETEYKCYGSGKPMPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAM8</accession>
    <name>A0A6I9YAM8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nitrite reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106547941</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide. Could help transport oxygen and increase its availability to the metabolically active neuronal tissues, though its low quantity in tissues as well as its high affinity for dioxygen, which may limit its oxygen-releasing ability, argue against it. The ferrous/deoxygenated form exhibits a nitrite reductase activity and it could produce nitric oxide which in turn inhibits cellular respiration in response to hypoxia. In its ferrous/deoxygenated state, it may also exhibit GDI (Guanine nucleotide Dissociation Inhibitor) activity toward heterotrimeric G-alpha proteins, thereby regulating signal transduction to facilitate neuroprotective responses in the wake of hypoxia and associated oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Fe(III)-heme b-[protein] + nitric oxide + H2O = Fe(II)-heme b-[protein] + nitrite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:77711"/>
            <dbReference type="Rhea" id="RHEA-COMP:18975"/>
            <dbReference type="Rhea" id="RHEA-COMP:18976"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:77713"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the globin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920700.1">
        <property type="nucleotide sequence ID" value="XM_014065225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAM8"/>
    <dbReference type="GeneID" id="106547941"/>
    <dbReference type="KEGG" id="tsr:106547941"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050532">
        <property type="entry name" value="Globin-like_OT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46458">
        <property type="entry name" value="BLR2807 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46458:SF19">
        <property type="entry name" value="NEUROGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="14">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013920700.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="17121" checksum="34F84F1456B59698" modified="2020-10-07" version="1">MNRDANIVQFSAQYSIGGCPSLYFRLFDLDPDLLPLFKYNCKTFSTPQECLSSPEFLEHIRKVMLVIDAAVTHLENLHCLEDYLSNLGRKHQAVGVKVESFEAVGDSLLYMLEKCHGPAFKMNVREAWIKLYSTVVKAMSCGWKVPRKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB21</accession>
    <name>A0A6I9YB21_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GTPase-activating protein 18-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106548453</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921304.1">
        <property type="nucleotide sequence ID" value="XM_014065829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB21"/>
    <dbReference type="GeneID" id="106548453"/>
    <dbReference type="KEGG" id="tsr:106548453"/>
    <dbReference type="OrthoDB" id="27680at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016032">
        <property type="term" value="P:viral process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04391">
        <property type="entry name" value="RhoGAP_ARHGAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000118">
        <property type="entry name" value="Rho GTPase activating protein 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.375.10">
        <property type="entry name" value="Human Immunodeficiency Virus Type 1 Capsid Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045345">
        <property type="entry name" value="Gag_p24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008916">
        <property type="entry name" value="Retrov_capsid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008919">
        <property type="entry name" value="Retrov_capsid_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057323">
        <property type="entry name" value="RHG40/28/18_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF6">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00607">
        <property type="entry name" value="Gag_p24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19317">
        <property type="entry name" value="Gag_p24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25442">
        <property type="entry name" value="Ubiquitin_RHG40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47943">
        <property type="entry name" value="Retrovirus capsid protein, N-terminal core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="313"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="543"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013921304.1"/>
        </source>
    </evidence>
    <sequence length="656" mass="73767" checksum="485720040D7F1010" modified="2020-10-07" version="1">MVEGISNGYNLIPQDWKDLFRMLLTPAQYIVWNAEYQTEAIHAGNNSGGLYIPDQLYGVGAFTTIQQQATGTPPPAYSVIGRCVIKAFKRVPVSGKPSKSFAAIRQNAGETYSDFIDRLQEAIQRQVDNEEASGELMKRLAYEHANADCHCALQSIVNRGDYTLADMLKACADIDTNVHQMDALTAALQKGKFGKPKGTKGIFVIPGVIDSDYEGILMIQFEINVPMTLKQGDSWAQLVIVPYVVPAGVWQSTTPVWIEQWPLTQEKLIALHELVREQLAANHIEPTTSPFNTPVFVIKKKSESGLFGVPLLVLLEYDQKRIPGIRTPFIFQKLISQIEGGKLETEGLLRIPGVAARVKNLCQELESKFYEGTFNWDSVKQHDAASLLKLFIRELPQPLFTVEYLKAFQDVQKLPTKKHQLQALNLLVLLLPEGNRDTLKVLLEFLQRVIDHGDKNKMNLKNVAMVMAPNLFMFHGLGAKPTEQSEFVMAAGTANVMRFMIKYQNLLWTIPTFLVNQVRKQNAESQKKERKDKAMRKLLKKMAYDREKHEKQDKSISDQKSGNDADIPEGVIRVQAPHLSKVSMAIQLTGELKAGDIVARFLSQKSGTVQSLKKEELFLHEVGGNIGERCLDDDTFMKNLYQLNPNAEWIIKSRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB93</accession>
    <name>A0A6I9YB93_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protocadherin-15-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106548508</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921364.1">
        <property type="nucleotide sequence ID" value="XM_014065889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB93"/>
    <dbReference type="GeneID" id="106548508"/>
    <dbReference type="KEGG" id="tsr:106548508"/>
    <dbReference type="OrthoDB" id="10029135at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000047">
        <property type="entry name" value="protocadherin-15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000048">
        <property type="entry name" value="protocadherin-15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000057">
        <property type="entry name" value="protocadherin-15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF31">
        <property type="entry name" value="NEURAL-CADHERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="135"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="265"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="383"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921364.1"/>
        </source>
    </evidence>
    <sequence length="550" mass="61465" checksum="62AF1D3AB0594375" modified="2020-10-07" version="1">MVLHQSRCRGNSKIAHLRGYQVNVANYRVIELDLSYNEFSEKAGELFGQVLVNNESLEILNLSWNHLRLKGATALSNSLKVNGTLKILNLSWNGFGNDGAFALGEALKVNITLAELDISNNHISNDGAIKLSKGLEMNGSLRILKLTPVGTTIFTGFSGNRGATDIDDGPNGQIEYIIQYNPSDPTSNRTFDIPLTLSGAIVLRERLNYEEKSRYFLIVQANDRAQNLVERRTSTTTLTVDVLDGDDLGPMFLPCILVFNTRDCRPLTYQASLPELTDPARVNPINVTPPIQAIDQDRNIQPASDRPGILYSILVGTPEDYSQYFSMNGTTAKLRLLKPINRDFHQKFDLVIKAEQDNGHPLPAFANLHIEVLDENNQKPFFTKSTYDGFISESSPVGTTISDNQNLTSPLQIVALDNDVEETKDPLLHLFLNDYNTFFTVTQTGIARYLTLLQPVDREFQQLYTFLITASDGVQESFPVTVNIFVLDANDNSPTFSNISYNVKIYTDMRPGESVIKRYSHTMKECLMSGYSQWCCDSRTDVDAEINLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD94</accession>
    <name>A0A6I9YD94_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MARVEL domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MARVELD1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922024.1">
        <property type="nucleotide sequence ID" value="XM_014066549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD94"/>
    <dbReference type="GeneID" id="106549030"/>
    <dbReference type="KEGG" id="tsr:106549030"/>
    <dbReference type="CTD" id="83742"/>
    <dbReference type="OrthoDB" id="9938733at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF28">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="27"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013922024.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="19149" checksum="910081B23F21AB96" modified="2020-10-07" version="1">MPLPPPPSSPPPPCPASRESLSLNRPFLRSLLGGLRIAQLLFGAVCWITLAAHKYEGATYFAVFAAVLVWLLTLVLFGLSVLGRWTLIPVLGTRWLLTNILHDLLLGLIFFTAAAAIIGYKARSISYCTLPGYSQPCSYTAYLVAAIFAGKSAFLYLLSGLYSLVRRFQGHFDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDG4</accession>
    <name>A0A6I9YDG4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein O-GlcNAcase</fullName>
            <ecNumber evidence="15">3.2.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Beta-N-acetylhexosaminidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Beta-hexosaminidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Meningioma-expressed antigen 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">N-acetyl-beta-D-glucosaminidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">N-acetyl-beta-glucosaminidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">MGEA5</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H2O = N-acetyl-D-glucosamine + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:48876"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12251"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:90838"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="3.2.1.169"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H2O = L-threonyl-[protein] + N-acetyl-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:48892"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:12252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:90840"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="3.2.1.169"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. Interacts with CLOCK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyl hydrolase 84 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.169" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013922206.1">
        <property type="nucleotide sequence ID" value="XM_014066731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDG4"/>
    <dbReference type="GeneID" id="106549157"/>
    <dbReference type="KEGG" id="tsr:106549157"/>
    <dbReference type="CTD" id="10724"/>
    <dbReference type="OrthoDB" id="9975416at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102571">
        <property type="term" value="F:[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-threonine O-N-acetyl-alpha-D-glucosaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016231">
        <property type="term" value="F:beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000009">
        <property type="entry name" value="O-GlcNAcase BT_4395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.240:FF:000001">
        <property type="entry name" value="O-GlcNAcase like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000023">
        <property type="entry name" value="protein O-GlcNAcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.240">
        <property type="entry name" value="STAT, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051822">
        <property type="entry name" value="Glycosyl_Hydrolase_84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011496">
        <property type="entry name" value="O-GlcNAcase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13170">
        <property type="entry name" value="O-GLCNACASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13170:SF16">
        <property type="entry name" value="PROTEIN O-GLCNACASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07555">
        <property type="entry name" value="NAGidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52009">
        <property type="entry name" value="GH84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH84" evidence="21">
        <location>
            <begin position="69"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="506"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS52009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013922206.1"/>
        </source>
    </evidence>
    <sequence length="924" mass="103945" checksum="4756A13F69DE3B84" modified="2020-10-07" version="1">MAADGSPGVDLAGMVAETRGEPPGTSLPTSTTVSSPRSESPPTRPDPSGPEAVPESGSGAVGNAGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWGLNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLIAAAQEYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCKCFALLFDDIDHNMCAADKEVFSSFAHAQISITNEIYQYLGEPETFLFCPTEYCGTFCYPNVAQSPYLRTVGEKLLLAIDVLWTGPKVVSKDISVESIEEVSKIIRRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANFVAIHTLATWYKSNMNGVRKDVVMTDTEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLSLTEWLQEFAVPHQYSSRQVAHSGAKTSVVDVPLIAPSTLNSTTVVTTVYQEPIMSQGADLSNESQTLVKEEEEKKQLDEEPMDMVVEKQEDADKNISQILTDISEAKMSEELKPMDTDKESITESKSPEMSMQEDCGNDIAPMQTDEQINKEQFVPGPHEKSLYAIEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWQTRAAKFEEMCSLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSQSSAQFLSGDQEPWAFRGGLAGEFQRLLPIEGANDLFFQPPPLTPTSKIYTIRPYFPKDEASVYKICREIYDDGTDQPFHSQPDLIGDKLVGGLLSLSPDYCFVLEDEDGICGYALGTVDVTPFIKKCKIAWIPFMQEKYSKPSGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF95</accession>
    <name>A0A6I9YF95_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Endonuclease V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ENDOV</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Active against both single-stranded and double-stranded RNAs. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Interacts with PABPC1; the interaction is RNA-dependent and stimulates ENDOV activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the endonuclease V family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922837.1">
        <property type="nucleotide sequence ID" value="XM_014067362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF95"/>
    <dbReference type="GeneID" id="106549656"/>
    <dbReference type="KEGG" id="tsr:106549656"/>
    <dbReference type="CTD" id="284131"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06559">
        <property type="entry name" value="Endonuclease_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2170.10:FF:000002">
        <property type="entry name" value="Endonuclease V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00801">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 19" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="255"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013922837.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31759" checksum="C63A4C7BABA49E62" modified="2020-10-07" version="1">MADGTGSREEERETLRRWERQQIQMKEDVIEQDTEEWQSNFSFTGLERIGGMDLSYLKEDATCACASLVVLSYPELEVLYEDCYVVAVNAPYVAGFLAFREVPFLLEAVQRLETQKPGLKPQVLLVDGNGILHHRGFGIACHLGVLTGLPCIGVAKNLLQVEGLANDEFHKKQIQDLQAGGDTFPLKSTSGKILGMALRSCAKSTKPVYISVGHKISLLSAVRLVHSCCKYRIPEPIRQADIRSREYIRKHMEVNIPVSPPPPEKGQDLESARLWEADKDEGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG89</accession>
    <name>A0A6I9YG89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106549791</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923009.1">
        <property type="nucleotide sequence ID" value="XM_014067534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG89"/>
    <dbReference type="GeneID" id="106549791"/>
    <dbReference type="KEGG" id="tsr:106549791"/>
    <dbReference type="OrthoDB" id="1104503at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000103">
        <property type="entry name" value="Histone H2A type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="17"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="15">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923009.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="23153" checksum="44CCD41D58A14660" modified="2020-10-07" version="1">MSGRGKKHPKPALPARITKTKKCGLQFPVARIHRFLKQGHYAERVSPGASVYLAAVLEYLSAEVIELAGSAAHQNKKKRIGPRHIQMAIRNDEELNKLFSHVTIPEGGVLPNIQAILLPISEHSGFPASGELGTSLAELGAVLAEFGMALCWFASESAWKVLLQLERSCGLNDFAAWAGGIRRPVGRVPPPHEWRSSGYQQAEGHLAWRAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGA2</accession>
    <name>A0A6I9YGA2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sulfotransferase</fullName>
            <ecNumber evidence="13">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">HS3ST1</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucosaminyl-[heparan sulfate](n) + 3'-phosphoadenylyl sulfate = 3-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15461"/>
            <dbReference type="Rhea" id="RHEA-COMP:9830"/>
            <dbReference type="Rhea" id="RHEA-COMP:9831"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:58388"/>
            <dbReference type="ChEBI" id="CHEBI:70975"/>
            <dbReference type="EC" id="2.8.2.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013922939.1">
        <property type="nucleotide sequence ID" value="XM_014067464.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922940.1">
        <property type="nucleotide sequence ID" value="XM_014067465.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922941.1">
        <property type="nucleotide sequence ID" value="XM_014067466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGA2"/>
    <dbReference type="GeneID" id="106549744"/>
    <dbReference type="KEGG" id="tsr:106549744"/>
    <dbReference type="CTD" id="9957"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000674">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF18">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636642" description="Sulfotransferase" evidence="14">
        <location>
            <begin position="18"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="15">
        <location>
            <begin position="57"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="10">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013922939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013922940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013922941.1"/>
        </source>
    </evidence>
    <sequence length="309" mass="36021" checksum="7534810D6CAB3F32" modified="2020-10-07" version="1">MAFLLLSAYLLLTPAQAAPVENGTLVVETLTSQALLGNKSEHYLAQVRPPGTSRQIPQTIIIGVRKGGTRALLEMLDVHPNIVVAATEVHFFDWEENYVKGIDWYRSLMPLSYENQITIEKTPGYFTSPQAPERIHDMNSSIKLLLILRDPTERVISDYTQVYYNRLENHKPVQPFEDVVIKNGVLNTKYKAIQRSLYDVHMGRWLKYFHLDQIHIVDGNTLIRDPLPELQKVERFLNLPSKILSSNFYFNQTKGFYCIRSDGRERCLHESKGRPHPVVNSTVLEQLYSYFREHNERFYRMINHSFDWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGR2</accession>
    <name>A0A6I9YGR2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Centrosomal protein of 128 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CEP128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923377.1">
        <property type="nucleotide sequence ID" value="XM_014067902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGR2"/>
    <dbReference type="GeneID" id="106550107"/>
    <dbReference type="KEGG" id="tsr:106550107"/>
    <dbReference type="CTD" id="145508"/>
    <dbReference type="OrthoDB" id="10046318at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026652">
        <property type="entry name" value="CEP128"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46657">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 128 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46657:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 128 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="415"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="629"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="746"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="880"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013923377.1"/>
        </source>
    </evidence>
    <sequence length="954" mass="112654" checksum="C2AB591C305AD28F" modified="2020-10-07" version="1">MADSSSDSDNIRHRIGRRGPIRATRIRNHGTEDVAEKIHTLANTLQDANWNLRHVDQMLGQYREYNNEQAETIANLKETLEESVGHLKNQRLLRNSGVRSASLSSLYASDLDGGSPEVHHVKPTSPLRDYSATLGIRQRRSRSAVRFVDQRGKLDQLHSLHQSLRDVSSEQIRLGEDLTRELSRRNRSDAEIKKKLEDISGRLDESQRQETVSERVERRLQEIEREIRSERQLVERRQDQLGHMSVHLQEALKQQDAKGNGTEIVLKNRIAKMEDEKSKLEQVLEHSQRKLDHSESNREILLHQIEDLRSQLLRAEEDRSTLQHQISQVSKHHQSHLNEQQDDRRKQKVVERSDPEKQEMEKQIWELRAQLNHSAVMSEIEELQRCVERKDKEKLQLVMQMEALTADLEKRDNQQQRMMSQLKEIQSNYKICENECRAAELQVTELGQQLEESIKEAEKYLAEFKQSEVLRLETEKKKEELKLKAQESIRCWKLRCKKLEHEKGKQSELHSQLMEKNKLVLKEKDELKSQLLSTMHQMENLQKELTDVLEKRARQEEELHSKEMKLSEARSLQMAFEQDTRDVRKTMDNLENKLQKESLIQTQIRTEKQHLEEELANINVIHEKDQARLSEMQEVIKNLSAVRADLANQVAEEKKSKKEMAKLVMELQKQIESVQEEMTTINKQLKLERDVHKQELGELQSELKQMKTKHDQSVQEMMKLFNQEKEEAVNHIGKLKSELTEERNFVKAHRRQVEKMKIECDKLSLELTHKEEENAKIKRKCNIIKQELDEKNKIINSEDDHLRRLDGERLQLHDQLQSLKTEQESIYRMIGSEVDATCQVFSRDSMEKMTTTSLTSGILNDPHRWLAETKAKLRWLCEEVKERQTKEKKLRHQLQQSREQLKQLSLSKETEHQNLIDQIEKQEQLLEEVYQEKKHLLEKTIRKEEEICTLQVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH51</accession>
    <name>A0A6I9YH51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Homeodomain-interacting protein kinase 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106549974</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923210.1">
        <property type="nucleotide sequence ID" value="XM_014067735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH51"/>
    <dbReference type="GeneID" id="106549974"/>
    <dbReference type="KEGG" id="tsr:106549974"/>
    <dbReference type="OrthoDB" id="10030361at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF45">
        <property type="entry name" value="HOMEODOMAIN-INTERACTING PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026884414" evidence="7">
        <location>
            <begin position="22"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="502"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="541"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013923210.1"/>
        </source>
    </evidence>
    <sequence length="805" mass="88043" checksum="1F0D8309B76296E3" modified="2020-10-07" version="1">MWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPVEQLLNLGTKTTRFFCREKDAPYSGWRLKTLEEHEGETGMKSKEARKYIFNCLDDIVHVNMVMDLEGSDLLAEKADRREFVSLLKKMLLIDANLRITPVDTLSHPFVTMKHLLDFPHSNHVKSSFHIMDVCKYRSNSYEISNCNKTIHLGPVAASNALGLMPNFTKIGTVQNQTLAATGHAVMHPGIPLQAGTAQFGCSDAFHQTLIICPPAVQGIPANHGKTNGYSVRVDNAVPLVTQAPTLLQIRGVLSQTWSNRSQQLLFPTWQHITSVAPATTLASDSVAGPQRLGEWGKMIPHGNHYNSVMTQPFLTNQITLSAPQPISVGIAHIVWPQPTPTKRNKARNNTLQNTNIQNSAFVSPKIINMKADEKLSLVETQDNHNSERQENSYCMPSVRQVPDPSVSNKQRQAIIIADSPSPAASVITISSDTDEDDAMQRHSVAECKGSLDCEACQSTLNIDRVCSLSSPDSTLSTSSSGQSSPSPCKRPNSISDDEQESGCDTVDGSPSSDSSGHDSPFIANHFSEASNKKTETRASANLEAKPTVCTVVVPPIRIQNRLNVDGQMTNSVNTCQPLVKRRSARVKTNHSSGLGNRQRKFMSAFHQQHSNLSRVQPFGSGSQEWNGNCGHWRQQTFIPNNVAGHAFSLPHGSPTHTSVHAQLAGNTHLGGQPAILPYPSSASLSTAAPVAHIFASPCTSRPILHHPSYSISHHGGIIHQIPVGINPRLLPSPNIQQTRYKPIFPPHSYIASPAYTGFPLSPTKLSQYPFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHW9</accession>
    <name>A0A6I9YHW9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein O-mannosyl-transferase 2</fullName>
            <ecNumber evidence="4">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">POMT2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013923564.1">
        <property type="nucleotide sequence ID" value="XM_014068089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHW9"/>
    <dbReference type="GeneID" id="106550225"/>
    <dbReference type="KEGG" id="tsr:106550225"/>
    <dbReference type="CTD" id="29954"/>
    <dbReference type="OrthoDB" id="5561486at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="120"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="252"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013923564.1"/>
        </source>
    </evidence>
    <sequence length="538" mass="61166" checksum="CC702455EC973C89" modified="2020-10-07" version="1">MLNYAASFEGNGDMGLNPDNDLTLLLSFYSWDETHFGKMGSYYINRTFFFDVHPPLGKVTLGKHFLARALCLIVLPLVLYTALFAVHFAVLNRSGPGDGFFSSGFQSRLIGNNLHNVSVPEYVAYGSVITLKNLRMAGGYLHSHWHLYPEGVGARQQQVTAYLHKDLNNLWIIRKHNLNRGFLTHLLISLLGEMPIQSSVILEWTLPKSSGLYPVEILKNSLVFSNILLSVENGSGDSNDFWRIEVVGRKDGKRIKVLRSQIRLVHLATTCILGSTGKTLPKWGWEQVEVTCTPYQKETPNTLWNIEEHINSRLPNISLDVLKPSFPEILLESHIVMIRGNSGLKPKENEVTSKPWHWPINYQGLRFSGVNETDYRVYLLGNPVIWWVSLIAIGLYLMMIIFISVAVKRGVQLTAEHKELARVLQHGGGRIILGWFLHYFPFFLMGRVLYFHHYFPAMLFSIMLTGITWDVFLKFCAWFFSSSIQAGKIYNYGIAVLVVLSVYSFYLFHPLSYGMTGPLASDPSSSMAGLKWLETWEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKL0</accession>
    <name>A0A6I9YKL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Sigma non-opioid intracellular receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Sigma 1-type opioid receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SIGMAR1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Regulates calcium efflux at the endoplasmic reticulum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The C-terminal helices form a flat, hydrophobic surface that is probably tightly associated with the cytosolic surface of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ERG2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924772.1">
        <property type="nucleotide sequence ID" value="XM_014069297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKL0"/>
    <dbReference type="GeneID" id="106551240"/>
    <dbReference type="KEGG" id="tsr:106551240"/>
    <dbReference type="CTD" id="10280"/>
    <dbReference type="OrthoDB" id="347124at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006716">
        <property type="entry name" value="ERG2_sigma1_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868">
        <property type="entry name" value="SIGMA 1-TYPE OPIOID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868:SF1">
        <property type="entry name" value="SIGMA NON-OPIOID INTRACELLULAR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04622">
        <property type="entry name" value="ERG2_Sigma1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013924772.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25674" checksum="657E82956F72308F" modified="2020-10-07" version="1">MRWLPGPSSGWAGRGLRVAAAVVAAALLFQALRGWVSSRRYEFEPEEIALLAKHHAGLDHELAFAKIIVELRRRHPGHILPDEDLRWVFVNAGGWMGSMCLLHASLSEYVLLFGSAVDTSGHSGRYWVDIYDTLISGTFWKWREGTTKREIYYPGDTTIHLSGEGTSVQWSAGTWMVEYGRGFIPSTLAFALADTIFSTQDFLTLFYTLQVYAKALLLEANTYFSSTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMC9</accession>
    <name>A0A6I9YMC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Centromere protein J-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106551908</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925548.1">
        <property type="nucleotide sequence ID" value="XM_014070073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMC9"/>
    <dbReference type="GeneID" id="106551908"/>
    <dbReference type="KEGG" id="tsr:106551908"/>
    <dbReference type="OrthoDB" id="10252174at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061511">
        <property type="term" value="P:centriole elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026581">
        <property type="entry name" value="TCP10L/CENPJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058029">
        <property type="entry name" value="Tubulin-bd_CENPJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10331:SF23">
        <property type="entry name" value="CENTROMERE PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10331">
        <property type="entry name" value="T COMPLEX PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25779">
        <property type="entry name" value="Tubulin-bind_CPAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENPJ tubulin-binding region" evidence="4">
        <location>
            <begin position="317"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="653"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="822"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="132"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="873"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="664"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="847"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="859"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013925548.1"/>
        </source>
    </evidence>
    <sequence length="968" mass="110004" checksum="D4D74040DDF9B25B" modified="2020-10-07" version="1">MAISTDLNWEENLVSDWMSNAARAGVILNPAFPGLKTTRAISTESVLSGHGVNLMHRSSSISPSEDTMHQEQTLDLKSSFEEKVAVSKTASASNREQTLMVKDVPAQWTATETKPGSDQLDDLDEGYCTELLLQKLEQLKELQHHKKEILRRQQMEQIQRLMEEQQNLLSLVAEQQEHTGSPAIKDDGTQNYPGSVCLSPVPYFPQCPSRGNTDWDSTSRDSSPADQRDYLQNFNHNQILKKSHSADPFYKKQPLDISLKGNNELKLEDQKSISGKTKQSPEPSLQGNIEGDSDSEDQRNTEASKILTAGKCSGAVNEEERPIVASMQERKQTFEEFLEEQIRLEEQRLNEEATLKVAEKFQKPVSKQPFLKRGEGLNRYTNAKYKVAKEKESKTILQPDPFEIKNTIKADKQPLHRKTASSNKISDNFVPKLVNQNIKTKKNLSFSARKTTRLRNGTGKGISSSARQHTNEMKKEVLRNSLRPEINGEKEKNEENVRPLAKLHEIDAKLPAGKEAPSEVTEFLINPRERDQELSFELSFQKKLGNWEAEKEKEKKELDEFLFLEQAADEVSFASNSSLVIRILDQGQQISSGHRLSSTPIKPRLKEQQMNVLNVTAAANLNGKKWVSQASIQESHKDNSQVPEPFMFHRSSVDYESSSSAECQDSELDEENSDESSEMTSESEEDFEITIKPASEGAEKMTLKNQSNCPEFSECREQLESTENTKSPVSMEKEDGGQSNELCRDSTYPSVFCRDRNKFEFDDEISWTDFENSNLCTHIPDGDMLSKVPLTPDCSKTPFPDKVIKRKIAMVKKGDALLKQNMTVNEETEPPTSDLMLKLFPSLKPKPKADAQSRHESKSNMTQDESSGESVRSQLLKEKLVELEMEIERFRTENASLSKLREEHETALENIRKEKSAFEHHKTQELARIEELKKEEARKLQKERKVFEKYAQAARAIPDKKERDEIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNI1</accession>
    <name>A0A6I9YNI1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 397-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106552151</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925827.1">
        <property type="nucleotide sequence ID" value="XM_014070352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNI1"/>
    <dbReference type="GeneID" id="106552151"/>
    <dbReference type="KEGG" id="tsr:106552151"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003519">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003000">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001442">
        <property type="entry name" value="zinc finger protein 696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000933">
        <property type="entry name" value="zinc finger protein 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="14">
        <location>
            <begin position="138"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="551"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="576"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925827.1"/>
        </source>
    </evidence>
    <sequence length="601" mass="68451" checksum="55ADC1BA600B9429" modified="2020-10-07" version="1">MEKVPAGLGSTETSEQAGRERFAIQPRIRWELPHGAMLQPENMPTQWWEYLKALPSPAMVWSSPGLPDMTSWDDLITFERVFGVRQWPRGDGVTTLKPAFRAEGQQALSGKSARGKKACGKVKAIFLHENLPSTELHRLRFRQFCYQEAEGPREVCGRLRELCHCWLEPERRTKEQIVELLILEQFLTVLPQDIQNQVRERSPETCAQAVALAEGFILRQQEEKQQEVQGTRSSQDVAVDFSKARKVSVEVRQQQSGTRRRRGDIVNLSMGVNLASKVEQQHLECNEKGSVTAHPVSGGTQRREKSHVCIECGKSFTRPSDLAKHLNVHTGERPYLCVECGKSFSQLSHLTRHQKIHSGEKPHICSECGDTFSQRAYLVSHQRSHSGEQPYHCSYCQKCFSHQSALKIHERNHMGQKPYACTDCGKRFVSSSSLTTHRRIHTGEKPHACGVCGKRFIQHSNLVSHQRTHSGEKPFCCKVCSRKFAYPSDLTRHQKIHTGEKPYACDECDRRFTRLSDLITHQRIHTGEKPYRCLECGRKFRQRGVLVKHQRCHSKENPKGSEALQTTESQAKSPFKTHEVKLVKELGDQEEEPSLSLSPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNL6</accession>
    <name>A0A6I9YNL6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">LOW QUALITY PROTEIN: Fanconi anemia group I protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FANCI</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925315.1">
        <property type="nucleotide sequence ID" value="XM_014069840.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551695"/>
    <dbReference type="KEGG" id="tsr:106551695"/>
    <dbReference type="CTD" id="55215"/>
    <dbReference type="OrthoDB" id="195089at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026171">
        <property type="entry name" value="FANCI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029312">
        <property type="entry name" value="FANCI_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029308">
        <property type="entry name" value="FANCI_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029305">
        <property type="entry name" value="FANCI_S1-cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029315">
        <property type="entry name" value="FANCI_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029313">
        <property type="entry name" value="FANCI_S3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818">
        <property type="entry name" value="BC025462 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818:SF0">
        <property type="entry name" value="FANCONI ANEMIA GROUP I PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14680">
        <property type="entry name" value="FANCI_HD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14675">
        <property type="entry name" value="FANCI_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14674">
        <property type="entry name" value="FANCI_S1-cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14676">
        <property type="entry name" value="FANCI_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14677">
        <property type="entry name" value="FANCI_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FANCI solenoid 1 cap" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI solenoid 1" evidence="2">
        <location>
            <begin position="110"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI solenoid 2" evidence="3">
        <location>
            <begin position="321"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI solenoid 3" evidence="4">
        <location>
            <begin position="634"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013925315.1"/>
        </source>
    </evidence>
    <sequence length="890" mass="100948" checksum="5CF6978DA1F1B5C9" modified="2020-10-07" version="1">MDQKILLLEAEGNLEGLQSCLQSLNEKELNDLITKHALRKKAIGAVLRGMFKGSPCSQQKGVSRRLSIYKHCIELVESGDLQVEIASEIIRLLMLEIQNGRGFVCYFLDRWSPQYVIHLTSMFRDVPLSKEELQFVVEKILRMFSKLDLQEIPPLVYQLLLLSSKGSKKNVLEGIVNFFNQMDKKQKEEQRDSESLELEEATVPLEQLRHVEGTIILHVVLAINLDHDLGKELIKYLKVLKKNDLXPFFTLALYGSMIKIQTFDTCRIFVQVDVHARYNAAANEAFCLEILGSLRRCLSQQADVRLTLYELCLDVFGWDHVTQGLVELGFILMDSYGPKRPFGGKATETNHILPKTPAQQVCKLGADILLETFKVHEPIRSEILEQILNRVITNAGVPIAHFIDLLSSIILSAPLVLQNSSSNVTEAFEHLSFLPLDTVQGFLKAVQQINLFCLLQQIKQYYEAEPDLLPPLKLEKCILAQRDQIFLQEPLAHLLCCIQHCLAWYTNVLTVHQQTEDDQEEEEEDRSFQQELDKMLESITRRMIKSELEDFELDKSADFSQSTGVGLKNNIYAILVMGICEVLIEYNFSTGNYNKNKFEDVLSLFKCYASLCEILKEKAGKNKLSSTNKIARSFLSMGFVCTLLTSLFRDCAQSHEESLAVLRSSTEFMRYAVTVALQKVQQLDETGQTDGPEGENPGKMFQYLCKITRVLLWRYTSIPTSAEEFGKKDKGKSISLLCLEGLLRIFNTVPQLYPTKISQFLQALDIANEDDEEAAEVNVTEKAAFQIRQFQRSLTNLISNTEEDFSSKEALLLINILVMLSKLLDPSSSQFVQLLSWTVKLCKETSLEDAPCCKGLLSLLFTLHALVKSPVTILRDLAQDIHAQLGDIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNM5</accession>
    <name>A0A6I9YNM5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">StAR-related lipid transfer protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">STARD4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925872.1">
        <property type="nucleotide sequence ID" value="XM_014070397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNM5"/>
    <dbReference type="GeneID" id="106552184"/>
    <dbReference type="KEGG" id="tsr:106552184"/>
    <dbReference type="CTD" id="134429"/>
    <dbReference type="OrthoDB" id="196858at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070508">
        <property type="term" value="P:cholesterol import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010879">
        <property type="term" value="P:cholesterol transport involved in cholesterol storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042555">
        <property type="entry name" value="StarD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47006">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47006:SF1">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013925872.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="14859" checksum="162D763234E1425C" modified="2020-10-07" version="1">MEDISDIDSLATRIKNTMIQYYNIDDNEWRVARKTKDVTVWRKPSEEFSGCLYKTEGIVEDVTNRIIDHIRPGPYRINWDSLITTMDIVEKYEENCCVMRYTTAGQLLNIIAPREFIDFSYTTNCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRV2</accession>
    <name>A0A6I9YRV2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Voltage-dependent calcium channel subunit alpha-2/delta-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926881.1">
        <property type="nucleotide sequence ID" value="XM_014071406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRV2"/>
    <dbReference type="GeneID" id="106552996"/>
    <dbReference type="KEGG" id="tsr:106552996"/>
    <dbReference type="OrthoDB" id="10054666at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990454">
        <property type="term" value="C:L-type voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF6">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Voltage-dependent calcium channel alpha-2/delta subunit conserved region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926881.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="34667" checksum="6AE2C91114E4A0B3" modified="2020-10-07" version="1">MVSKAVKITVNGKLLKPAVVGVKINLNSWMTNFTTITTKDQCKSGSELCGCEKNSPHIDCVILDDGGFLLMTNQDNYVPEIGKFFGEIDPGLMRNLINMSLYAFNKSYDYQSVCDPEDEPKQGAGLRSINVPTIADILHLGWWASAAAWSILHQLVWGLTFPRFLGAVEVEEEDFSGFPSKQSCITVQTQYFFGSDDKSFNGILDCINCSRLFHAEKISNTNLVFIMSDSKELCHHCDTRPLMQAEKPDEGPNPCEMVQHPRYRKGPDACFDNATNDSTECGGVSALSPSLLSMVGIQLILLWMLSGTRPCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS18</accession>
    <name>A0A6I9YS18_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Epidermal growth factor receptor substrate 15-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">EPS15L1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <location evidence="11">Coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926415.1">
        <property type="nucleotide sequence ID" value="XM_014070940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS18"/>
    <dbReference type="GeneID" id="106552622"/>
    <dbReference type="KEGG" id="tsr:106552622"/>
    <dbReference type="CTD" id="58513"/>
    <dbReference type="OrthoDB" id="524326at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000026">
        <property type="entry name" value="Epidermal growth factor receptor pathway substrate 15-like 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000074">
        <property type="entry name" value="epidermal growth factor receptor substrate 15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF69">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0168">Coated pit</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="13">
        <location>
            <begin position="17"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="13">
        <location>
            <begin position="127"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="275"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="13">
        <location>
            <begin position="276"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926415.1"/>
        </source>
    </evidence>
    <sequence length="493" mass="53911" checksum="E0EFF3C216C15CA5" modified="2020-10-07" version="1">MAALGPLGGGGAQLSASNPLYETYYKQVDPACTGRVGSSDAALFLKKSGLTDAILGKIWDLADPEGKGFLDKQGFFIAMRLIACAQSGHDVILSNLNLTLPPPKFHDNTSPLLLASTEAHWAVRVEERAKFDGIFESLLPVNGLLSGDKVKPVLMNSKLPLDVLGRVWDLSDIDKDGHLDRDEFAVAMHLVYRALEKEPVPSLLPPSLVPPSKRKKVPIFPGAVPVLPASPPPKDSLRSTPSHGSVNSLNSTGSLSPKHSLKQTQPSANWVVPLTDKIRYDEIFLKTDLDLDGFVSGQEVKDIFMHSGLSQNLLAHIWALADIRQMGKLNKDQFALAMYLIQQKVSKGIDPPQVLLPDMIPPSERTTPVQTLSGYLASVGTEISTLTEMRRVSKRGTLKNSAPPHQTSAKQCEFIDLDDSLIEQFVWGVRNVRLKRRLLACTDITLQQAVDEARATELSDSSTAEIQWCQQLIAPPPPKNLPHQQVGFLGRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS20</accession>
    <name>A0A6I9YS20_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Isochorismatase domain-containing protein 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">ISOC2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927343.1">
        <property type="nucleotide sequence ID" value="XM_014071868.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013927344.1">
        <property type="nucleotide sequence ID" value="XM_014071869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS20"/>
    <dbReference type="GeneID" id="106553388"/>
    <dbReference type="KEGG" id="tsr:106553388"/>
    <dbReference type="CTD" id="79763"/>
    <dbReference type="OrthoDB" id="269496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050993">
        <property type="entry name" value="Isochorismatase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119:SF3">
        <property type="entry name" value="ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="44"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013927344.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="14703" checksum="51B4574373EE7165" modified="2020-10-07" version="1">MSLARLGKVVPKSSILFLCDMQEKFRPNISYFPQIVSVAARMLKSTALDAMERGLDVHVVVDACSSRSQVDRIIALSRLRQSGAFLTTSEGLILQLVQDAAHPRFREIQKLIMDPAPDSGLLPLIKVPNPLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS21</accession>
    <name>A0A6I9YS21_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Guanine nucleotide-binding protein subunit alpha-13 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">GNA13</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(12) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926956.1">
        <property type="nucleotide sequence ID" value="XM_014071481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS21"/>
    <dbReference type="GeneID" id="106553054"/>
    <dbReference type="KEGG" id="tsr:106553054"/>
    <dbReference type="CTD" id="10672"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031752">
        <property type="term" value="F:D5 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000004">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000754">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000469">
        <property type="entry name" value="Gprotein_alpha_12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF85">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT ALPHA-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00440">
        <property type="entry name" value="GPROTEINA12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="58"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="196"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="221"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="290"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013926956.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="43912" checksum="853FD2B6499F49FC" modified="2020-10-07" version="1">MADFLPSRSSLSGCFPGCMLNSSEAEQQRRSKAIDKCLYREKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDWDRSAREEFRATIYSNVIKGVRVLVDAREKLHIPWGNPANQTNGDKMMAFDTRSEVVQGMVQTYTFLQYLPTVRALWADTGIQHAYDRRREFQLGESIKYFLDNLDKLGDADYLPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQKVSIRDYFSEFEGDPHNLTDVQKYLVDCFRTKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSG6</accession>
    <name>A0A6I9YSG6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RAD52 motif-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RDM1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927086.1">
        <property type="nucleotide sequence ID" value="XM_014071611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSG6"/>
    <dbReference type="GeneID" id="106553171"/>
    <dbReference type="KEGG" id="tsr:106553171"/>
    <dbReference type="CTD" id="201299"/>
    <dbReference type="OrthoDB" id="6287754at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.80:FF:000002">
        <property type="entry name" value="RAD52 motif containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057652">
        <property type="entry name" value="DSRM_RDM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040224">
        <property type="entry name" value="RDM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31164">
        <property type="entry name" value="RAD52 MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31164:SF1">
        <property type="entry name" value="RAD52 MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25517">
        <property type="entry name" value="DSRM_RDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="15"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927086.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29126" checksum="2D283C82761AC3C6" modified="2020-10-07" version="1">MAEIVEFRVPQGNGRTLLVLGLELEASEHALHLTFSAFGLLHSVRVHRNASVAGPGYHAFVKFYSARDASKAQQVCNGRSLFQKTALKVCICTRQRALAKQTLPLSSCKSQELANYYLGFNGWSSRIITLQNVPGFEEDEKEEGGTLPRPHHSKYLCVQELNIPSYGIYTRGIGVAELQVDPSQGFLTASRNAQKFAVQQALSDAFRKILFIILENGKLAVEYSCPENDLIDCLSEEELRGQIQVTELSFSQLDPLGEEEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTL8</accession>
    <name>A0A6I9YTL8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Plexin-B3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106553085</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926995.1">
        <property type="nucleotide sequence ID" value="XM_014071520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTL8"/>
    <dbReference type="GeneID" id="106553085"/>
    <dbReference type="KEGG" id="tsr:106553085"/>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000270">
        <property type="entry name" value="plexin-B3 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF33">
        <property type="entry name" value="PLEXIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027109990" evidence="12">
        <location>
            <begin position="27"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="13">
        <location>
            <begin position="15"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926995.1"/>
        </source>
    </evidence>
    <sequence length="641" mass="69982" checksum="68C3C3039A4A3661" modified="2020-10-07" version="1">MVLGRLLPPLMVTLMLLLPLYPAAQPSSYPLFVVPNGTFNHLAQARSTGGLYVGAVNGLYQLDSELRLLGYARTGPDQDSPECLPFRDVRECPQAQLMDNSNKLLLPNERSGELVVCGQLFQGVCEKRALTDITKVLYHPEDPGDNQFVAANEPKVSTVGLVGHHNDRDLLFVGRGLTAKLSGGIPPFTIRQLEGPQAFSNEGMGKLVVGDFSDYNNSYVGAFASGGYVYFLFFRRGAKAQMEYRTYLSRTCVDDTNLYSYVELPLECQHSQGQSYNLAQAMHLAPGFAEKDKILFVVLAVGQGSTAVPTGRTALCSYSLNAINAAMEKTRQLCYTTSGIGDNNDEEAAIEYGVTSRCSSLSKESPEIYPCGDEHTPSPIASRKPLVAEALLTSVPRLTAVAAMVETGHTIAFLGDGVGQLHKVYLNGSVAQVYSTMPTGQNSPVNSDLLLNSSGAYLYVMTASQVSKIPVSECPNFEDCTSCLHAEDPFCGWCVLQSRCTRKLECERHELANHWLWSYGDESQCLRVEQILPANQSRETQTEISLMVPNLPALGEGEYYHCAFGTQKSQAQLQDSWIRCLSPVPEQVPPIQEGKDHVTVTLALMFQDVVITSLGFSFYDCNAVSQLADSTPCGTCVDSPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTZ9</accession>
    <name>A0A6I9YTZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF domain-specific O-linked N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="2">2.4.1.255</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Extracellular O-linked N-acetylglucosamine transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EOGT</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12251"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:90838"/>
            <dbReference type="EC" id="2.4.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48908"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:12252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:90840"/>
            <dbReference type="EC" id="2.4.1.255"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 61 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013927459.1">
        <property type="nucleotide sequence ID" value="XM_014071984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTZ9"/>
    <dbReference type="GeneID" id="106553479"/>
    <dbReference type="KEGG" id="tsr:106553479"/>
    <dbReference type="CTD" id="285203"/>
    <dbReference type="OrthoDB" id="529273at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF148">
        <property type="entry name" value="EGF DOMAIN-SPECIFIC O-LINKED N-ACETYLGLUCOSAMINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="13">
        <location>
            <begin position="184"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927459.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="35868" checksum="F7E67F42099E85A1" modified="2020-10-07" version="1">MNTDAFLAEGQQKSPLQSWFAELQTYAEMNSRPLEDGSCDVIVEKPTYFMKLDAGVNMYHHFCDFVNLYITQHVTNTFSTDINIVMWDTSTYGYGDLFSETWKAFTDYEIIHLKSYDSKRVCFKEAVFTLLPRMRFGLFYNTPLISGCHGTGLFRAFSQHVLHRLNITQDGPKLTAVIFVSFYRELEFKEQLKITHNSDIFIGMHGAGLTHLLFLPDWAVIFELYNCKDERCYLDLARLRGIHYITWQQKDKVFPQDEGHHPTLGKHPKFTNYAFDVEEFVRLVLLAADHVSQHSKWPFRRKHDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUM9</accession>
    <name>A0A6I9YUM9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Neurofascin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NFASC</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927726.1">
        <property type="nucleotide sequence ID" value="XM_014072251.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106553696"/>
    <dbReference type="KEGG" id="tsr:106553696"/>
    <dbReference type="CTD" id="23114"/>
    <dbReference type="OrthoDB" id="10010359at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
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        <property type="entry name" value="neurofascin isoform X1"/>
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        <property type="entry name" value="PROTEIN SIDEKICK"/>
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        <property type="entry name" value="Bravo_FIGEY"/>
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        <property type="match status" value="6"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1197"/>
            <end position="1218"/>
        </location>
    </feature>
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            <begin position="1251"/>
            <end position="1260"/>
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            <begin position="1262"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023319"/>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_013927726.1"/>
        </source>
    </evidence>
    <sequence length="1331" mass="148617" checksum="D59A9FF8401C4BE0" modified="2020-10-07" version="1">MVIQRYLLTYMVVVLASYLHNLIRAIEVPRDPSIQDTLPQPPTITKQSAKDYIVDPRDDILIECEAKGNPTPTFSWTRNGKFFNVAKDPRVTMKHWSGTLIVDFRSGGRPEDYEGEYQCFARNDYGTAISNKIFLHVSKSPLWPKENLDIQEVHEGASLILACNPPPGLPPPAIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMQQDALTDYSCNARFAFTHTIQQKNPYTLKIITTRGIAERQPSFMYPIGTSSSQMVLRGEDLLLECIASGVPSPDIMWHKKGGDLPSGRIKLENFNKALHIANISEEDSGEYFCLASNKVGISRHSILVKVKASPYWLDEPENLILAPGEDGRLLCRANGNPKPSIQWLVNGELLESSAPNPSREVNGDTVMFRDIQSGSSGVYQCNASNEHGYLLANAFVSVLDIVPRILVPRNQLIKVIQNNRTLLDCPFFGSPIPTLRWFKNGQGSTLHGGNYKAHENGTLEIIRARKEDQGTYTCVATNLLGKAQNQVRLEIKDPTRIIEGPKDAIPKKGSTIHLVCQTRHDATLRLTVTWLKDDIPIYYGNRMKKEEDGLTIHGVSEKDQGYYTCVASTELDKDTARAHVTVLAPSSVNRMTPPPARPERPRDLELTDLAERSVRLTWIPGDDNNSPITDYIVEFEEDKFQPGTWHNHSRYPGNVNSAFLRLAPYVNYQFRVIAVNDVGSSYPSLPSERYHTNGARPEFNPSGVKGSGTAKNNMVISWMPLNATQVYGPNLKYIVKWRRRDTRESWNNRTVKGDVSQYTVAPTPIYTPYEIKVQALNDYGIATEPDIVIGYSGEDYPKAAPTDIRLKVVNSTAISLQWSKVYPETIQGQLKEYRAYYWRESSLLKNLWVSKKKQSLGFIGDRNRGIVSRLFPYSNYKLEMVVVNGRGDGPRSEILEFTTPEGVPSAPRYFRIRQPNLEAVTLEWEHPEHPNGILIGYTLRYQAFNGSKTGRTMVEVFSPNHTKFLLQRTDPISRYRFNLRGRTQIGEGEPATGDSPIPLNEVLPTSDVPTVAATTISETTVPTTITEVVTTTTEVITTTTTEAVTTTTEAATTTEPAIITTLVASTIADVGKLPPIWNVKISSNNNWANITWNHAFDADTEFVVEYSNSNKSVKSSPAKGFLELSDLIPGEKYIVRVFLKGNESVFSSETFHTSPAYTKNQGDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRANKDVPLDPDDQKVDNGSFDYRSLESDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKDETEGNESSEATSPVNAIYSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUV2</accession>
    <name>A0A6I9YUV2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Vacuolar protein sorting-associated protein 37B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">VPS37B</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927774.1">
        <property type="nucleotide sequence ID" value="XM_014072299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUV2"/>
    <dbReference type="GeneID" id="106553734"/>
    <dbReference type="KEGG" id="tsr:106553734"/>
    <dbReference type="CTD" id="79720"/>
    <dbReference type="OrthoDB" id="10004364at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039702">
        <property type="term" value="P:viral budding via host ESCRT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000003">
        <property type="entry name" value="vacuolar protein sorting-associated protein 37B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678:SF9">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="10">
        <location>
            <begin position="38"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="120"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927774.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="25169" checksum="CBF773571C015E14" modified="2020-10-07" version="1">MTLASNHSLAEGNLLYQPKLDALKATLTQKYQELQVLFEAYQIKKTKLDRQSNNASLETLLALLQTEGAKIEEDTENMAEKFLDGDVPLDAFIDEYQSQRKLAHLRRVKIEKLQEVVLKGPRRPPVPPPPVLQTSEANTPPSVMPRRNPPPPPPASAVPAGRFPTPFMAAMGHNPYPYPPLPPRTGAPPPPGHGPSPGYPNPFLALYPSTPPQRPPPRLPHNPGFIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUX2</accession>
    <name>A0A6I9YUX2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein sidekick-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106553457</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927435.1">
        <property type="nucleotide sequence ID" value="XM_014071960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUX2"/>
    <dbReference type="GeneID" id="106553457"/>
    <dbReference type="KEGG" id="tsr:106553457"/>
    <dbReference type="OrthoDB" id="114660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019955">
        <property type="term" value="F:cytokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050379">
        <property type="entry name" value="Type-I_Cytokine_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23036">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23036:SF151">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013927435.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="13684" checksum="9F4B239EE407D60F" modified="2020-10-07" version="1">MQNLSEASLTQYELDNLNKHRRYEIRMSVYNAVGEGPTSSPQEVFVGEAVPTAAPQNVVIQTITATQLDITWEPPPPETQNGDIQGYKRHFGCHCGKQDIELKGPLITVVVYFSSQVNFYAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVR2</accession>
    <name>A0A6I9YVR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serotonin N-acetyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aralkylamine N-acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">AANAT</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-arylethylamine + acetyl-CoA = an N-acetyl-2-arylethylamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55469"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:77827"/>
            <dbReference type="EC" id="2.3.1.87"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the acetyltransferase family. AANAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.87" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013928718.1">
        <property type="nucleotide sequence ID" value="XM_014073243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVR2"/>
    <dbReference type="GeneID" id="106554544"/>
    <dbReference type="KEGG" id="tsr:106554544"/>
    <dbReference type="CTD" id="15"/>
    <dbReference type="OrthoDB" id="30840at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030187">
        <property type="term" value="P:melatonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000021">
        <property type="entry name" value="Serotonin N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051635">
        <property type="entry name" value="SNAT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10908">
        <property type="entry name" value="SEROTONIN N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10908:SF0">
        <property type="entry name" value="SEROTONIN N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0471">Melatonin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="33"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013928718.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23439" checksum="7776D83D90BB68DF" modified="2020-10-07" version="1">MSMISALPFFKSSYLRSSQNSLRSQRRHTLPASEFRCLTPEDAIGVFEIEREAFISVSEDCPLHLDEIRHFLNLCPELSLGLFEEGQLVAFIIGSLWDQERLSQDALTLHKPDGTIVHIHVLAVHCTFRQQGKGSILMWRYLQYLCSLPYVRRATLMCEDFLIPFYTKCGFKVLGPCEITIGSLNFIEMNCAIQAHAFMRRNSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVZ0</accession>
    <name>A0A6I9YVZ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Structural maintenance of chromosomes flexible hinge domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927765.1">
        <property type="nucleotide sequence ID" value="XM_014072290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVZ0"/>
    <dbReference type="GeneID" id="106553728"/>
    <dbReference type="KEGG" id="tsr:106553728"/>
    <dbReference type="OrthoDB" id="10036779at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038892">
        <property type="entry name" value="SMCHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055109">
        <property type="entry name" value="SMCHD1_S5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22640">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22640:SF2">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22899">
        <property type="entry name" value="SMCHD1_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMCHD1 ribosomal S5" evidence="1">
        <location>
            <begin position="342"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927765.1"/>
        </source>
    </evidence>
    <sequence length="643" mass="74599" checksum="03326662B8E95824" modified="2020-10-07" version="1">MFRRPPRRALSTKEIFVITTTDRKKITSENFDQIVKDKMTLYLLQRVDQLLTSATKECIEFLPHYNTILKSGTYEYYDSKEQTPLPYALAELIDNSLSATSQNSGIRCIQLKLLFDDSQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFIQQDNIESDHSGYVIPQPVPRSLNSDISYFGVGGKQAAFFVGQSVRMISKPANSQDVHELVLSKEDFEQKEKNKEPVYRTYIRNRKPSDSSHIVNVDERFLHKLILEEKEKDSFTAVVITGMQPKHVRYLKNNFHHCIRELKHIYHYYIHGPNGNMTNLQMNEEMVSNNMVIEISLFEKGKTPQIVNLREIKDDMQSLYINTATDSFEFKARVEGDGIVEGIIRYHPFLYDKETYPISSSNDDEDEDEEEDCYIKEKRTRGEEAIFKCFWNGRLIPYTTIAGFEWCTLPKKRGTVPFECYNRISGVLFTNGKFEVTTNKLTFVELESKLKDKNTLFTRIVNGQEQRMKIDKEFALWLNNCHQTHDKQIKFTQFKEMAARTDQAYKGKQKPWATYEAIEWDGKIYKVGQLVRSIGRAPVLHGRIQNFYLYGNHDGDDFAPGGDVQIALEPQALYNEIKFIPISKLDRSVSEAELEKYIDEEMARFPDRLSVTWPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWP5</accession>
    <name>A0A6I9YWP5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DNA repair protein XRCC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">XRCC3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928391.1">
        <property type="nucleotide sequence ID" value="XM_014072916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWP5"/>
    <dbReference type="GeneID" id="106554273"/>
    <dbReference type="KEGG" id="tsr:106554273"/>
    <dbReference type="CTD" id="7517"/>
    <dbReference type="OrthoDB" id="1861185at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033065">
        <property type="term" value="C:Rad51C-XRCC3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071140">
        <property type="term" value="P:resolution of mitotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090656">
        <property type="term" value="P:t-circle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19491">
        <property type="entry name" value="XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058766">
        <property type="entry name" value="HHH_XRCC3_RAD51B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047348">
        <property type="entry name" value="XRCC3-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46487">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46487:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26169">
        <property type="entry name" value="HHH_XRCC3_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47789">
        <property type="entry name" value="C-terminal domain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="10">
        <location>
            <begin position="91"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013928391.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="38680" checksum="2A94E312804BAEE1" modified="2020-10-07" version="1">MLLNIVWLCICEEMDWDKFDMNPRVIAAIKKANITSIKDIFHLSGSDLQRLTKLSKTDVQYLLRTISTTLRKNSVFTALQLFQNKAPSTSQGQKLSLGCPFLDGLLRGGIPRTGITEIAGESSAGKTQIALQLCLSVQYPYRYGGLESATFLNINREWVIGEEVVSLTRTVARRDTFHECITKRLSLLLSRGMVRLIIIDSIAALFRCEFSATDSLQKAKYLQKFGAKLHRLSCTFQTPIICINQVTDAINVREDAVWNTQSCSARTVVPALGITWSNQLLMRLMVCRTVNQALTGGETLYSKSELRMLRIIFAPHLPQHFCYYTVNLEGVKGVGKISHDYEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXX4</accession>
    <name>A0A6I9YXX4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transitional endoplasmic reticulum ATPase</fullName>
            <ecNumber evidence="2">3.6.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Valosin-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106554929</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013929152.1">
        <property type="nucleotide sequence ID" value="XM_014073677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXX4"/>
    <dbReference type="GeneID" id="106554929"/>
    <dbReference type="KEGG" id="tsr:106554929"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF63">
        <property type="entry name" value="TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="11">
        <location>
            <begin position="26"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="10">
        <location>
            <begin position="126"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="238"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="511"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="770"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013929152.1"/>
        </source>
    </evidence>
    <sequence length="807" mass="89586" checksum="28AB79E0C2B10610" modified="2020-10-07" version="1">MPASGGDPKGEDYSTAILRQKHRPNRLIVDEAANEDNSILSLSQAKMEELHLFRGDTVLMKGKKRRETVCIVLTDDSCQNEKIRMNRVTRNNLRVRLGDVVSVQACPDVKYGKRIHVLPIDDTIEGLTGNLFEVYLKPYFLEAYRPVHKGDIFLVRGGMRAVEFKVVEVDPSPHCIVAPDTIIHCEGEPIKREDEEESLNDVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLVARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRSHVIVMAATNRPNSIDPALRRFGRFDREIDIGIPDSVGRLEILQIHTKNMKLADDVDLEKVGNETHGHVGADLAALCSEAALQAIRKKMSVIDLEDDTIDADILNAMAVTMDDFQWALGQSNPSALRETVVEVPQVSWDDIGGLQEVKRELQELVQFPVEYPDKFLKFGMTPSRGVLFYGPPGCGKTLLAKAIANECQANFVSIKGPELLTMWFGESEANVRDVFDKARQAAPCILFFDELDSIAKSRGGGAGDGGGAADRVINQILTEMDGMTNKKTVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILQANLRKSPVAKDVELNYLAKITHGFSGADLTEICQRACKLAIREAIEMEIKAERERQRQKCAAMDDDYDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGNFRFPAGKQTTGGDGSSGSNSGQGSLFQEEGEDDLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY44</accession>
    <name>A0A6I9YY44_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Trans-Golgi network integral membrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TGOLN2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929598.1">
        <property type="nucleotide sequence ID" value="XM_014074123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY44"/>
    <dbReference type="GeneID" id="106555301"/>
    <dbReference type="KEGG" id="tsr:106555301"/>
    <dbReference type="CTD" id="10618"/>
    <dbReference type="OrthoDB" id="5846619at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037645">
        <property type="entry name" value="KCT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16502">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16502:SF0">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17818">
        <property type="entry name" value="KCT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026657271" evidence="3">
        <location>
            <begin position="21"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929598.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="54598" checksum="7B8AA1FE9EA1AED9" modified="2020-10-07" version="1">MAACWSWCLLLCWLAAWGSAAPLGAGKSPGTNAQELSSDHLPLTNLNQGASEPEPLQVVQSNIKNSQQEPPQQGSDKTMETAKSSIGHKDSQPGAIGSNRPQEDIGKSQGDQTGTSHTSSLLKSNIKASEQREDPSIKNHNAGLSAQDASPKSGLVQPKKIEKGIVNSEDINALEPKQSQDPSAKDQNPQSAQQPGPAKVEAAKGMADIDKPKEAQTETSDASLPELTKASKQGEDPSIKGPNTQPGDSAQEPSKLNPVQTGNTQKEKDHTQESKTGVSQPGVNVKESEQSPGLQKKDQNPQSVSSLQEPASGKINSPEATNVQKDVADEASIPESKPKEPNQGGDLLIKDLNAQGSSKSKPADAKDAESKTEISDQDVSEQETNDEESKQAEDQDFPLDQHPQKLNPVESKKNEEEVVDPGDEQEKGLGDEQKKSAKHEEESEGTPRNGSENSHFFAYLVTTAIIVAALYIAYHNKRKIIAFALEGKKSKITRRPKSSDYQRLDQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZ42</accession>
    <name>A0A6I9YZ42_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="40">Fragile X mental retardation protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="40" type="primary">FMR1</name>
    </gene>
    <organism evidence="39 40">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="40" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="34">Multifunctional polyribosome-associated RNA-binding protein that plays a central role in neuronal development and synaptic plasticity through the regulation of alternative mRNA splicing, mRNA stability, mRNA dendritic transport and postsynaptic local protein synthesis of target mRNAs. Acts as an mRNA regulator by mediating formation of some phase-separated membraneless compartment: undergoes liquid-liquid phase separation upon binding to target mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors. Binds poly(G) and poly(U), and to a lower extent poly(A) and poly(C). Forms a cytoplasmic messenger ribonucleoprotein (mRNP) network by packaging long mRNAs, serving as a scaffold that recruits proteins and signaling molecules. This network facilitates signaling reactions by maintaining proximity between kinases and substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="35">Homodimer. Heterodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Filopodium tip</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell projection</location>
            <location evidence="9">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Chromosome</location>
            <location evidence="7">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
            <location evidence="8">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="31">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="33">Presynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Synapse</location>
            <location evidence="32">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the FMR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928855.1">
        <property type="nucleotide sequence ID" value="XM_014073380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZ42"/>
    <dbReference type="GeneID" id="106554661"/>
    <dbReference type="KEGG" id="tsr:106554661"/>
    <dbReference type="CTD" id="2332"/>
    <dbReference type="OrthoDB" id="424249at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048170">
        <property type="term" value="P:positive regulation of long-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099577">
        <property type="term" value="P:regulation of translation at presynapse, modulating synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22506">
        <property type="entry name" value="KH_I_FMR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22509">
        <property type="entry name" value="KH_I_FMR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22512">
        <property type="entry name" value="KH_I_FMR1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20471">
        <property type="entry name" value="Tudor_Agenet_FMR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20474">
        <property type="entry name" value="Tudor_Agenet_FMR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000001">
        <property type="entry name" value="Fragile X mental retardation 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000002">
        <property type="entry name" value="Fragile X mental retardation 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000004">
        <property type="entry name" value="Fragile X mental retardation 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000054">
        <property type="entry name" value="Fragile X mental retardation protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040148">
        <property type="entry name" value="FMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022034">
        <property type="entry name" value="FMR1-like_C_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032196">
        <property type="entry name" value="FMR1_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040472">
        <property type="entry name" value="FMRP_KH0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047438">
        <property type="entry name" value="KH_I_FMR1_rpt1"/>
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        <property type="entry name" value="KH_I_FMR1_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047431">
        <property type="entry name" value="Tudor_Agenet_FMR1_rpt1"/>
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        <property type="entry name" value="Tudor_Agenet_FMR1_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041560">
        <property type="entry name" value="Tudor_FRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10603">
        <property type="entry name" value="FRAGILE X MENTAL RETARDATION SYNDROME-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10603:SF4">
        <property type="entry name" value="FRAGILE X MESSENGER RIBONUCLEOPROTEIN 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16098">
        <property type="entry name" value="FXMR_C2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12235">
        <property type="entry name" value="FXMRP1_C_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF17904">
        <property type="entry name" value="KH_9"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF18336">
        <property type="entry name" value="Tudor_FRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51641">
        <property type="entry name" value="AGENET_LIKE"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="28" id="KW-0966">Cell projection</keyword>
    <keyword evidence="30" id="KW-0137">Centromere</keyword>
    <keyword evidence="12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="25" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="19" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="22" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="26" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0678">Repressor</keyword>
    <keyword evidence="29" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="21 36" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="23" id="KW-0770">Synapse</keyword>
    <keyword evidence="16" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="20" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Agenet-like" evidence="38">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet-like" evidence="38">
        <location>
            <begin position="63"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="377"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="37">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="37">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="37">
        <location>
            <begin position="457"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="37">
        <location>
            <begin position="471"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="37">
        <location>
            <begin position="485"/>
            <end position="506"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="37">
        <location>
            <begin position="524"/>
            <end position="534"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="37">
        <location>
            <begin position="535"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="37">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
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            <dbReference type="ARBA" id="ARBA00004484"/>
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            <dbReference type="ARBA" id="ARBA00004552"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006633"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022454"/>
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    <evidence type="ECO:0000256" key="14">
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
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    <evidence type="ECO:0000256" key="21">
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            <dbReference type="ARBA" id="ARBA00022884"/>
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    <evidence type="ECO:0000256" key="22">
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            <dbReference type="ARBA" id="ARBA00022902"/>
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            <dbReference type="ARBA" id="ARBA00023018"/>
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    <evidence type="ECO:0000256" key="24">
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023187"/>
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    <evidence type="ECO:0000313" key="40">
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            <dbReference type="RefSeq" id="XP_013928855.1"/>
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    <sequence length="564" mass="63621" checksum="58F60F70378DC11F" modified="2020-10-07" version="1">MEGLPVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPERQIPFHDVRFPPPTGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKESAHKDFKKAVGAFSVTNDPEAHQLIILSINEVTTKRANMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFVVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSYLEFAEDVIQVPRNLVGKVIGKNGKLIQEIVDKSGVVRVRIEAENDKNIPQEEGMVPFVFVGTKDSITNATVLLDYHLNYLKEVDQLRLERLQIDEQLRQIGATSRPPPNRPDKEKVYMADDGPGLGRGGRPYRNRGHSRRGPGYASGTNSEASNASETESDHRDELSDWSLAPAEEERDSYLRRGDGRRRGGGARGQGGRGRGGFKGNEDQSRTDNRQRNSRDAKGRTDGSLQIRIDCNNERSVHTKTLQNTSSEASRLRTGKDRNQKKEKPDSGESQQPLVNGVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZF2</accession>
    <name>A0A6I9YZF2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PRDX2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013928980.1">
        <property type="nucleotide sequence ID" value="XM_014073505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZF2"/>
    <dbReference type="GeneID" id="106554774"/>
    <dbReference type="KEGG" id="tsr:106554774"/>
    <dbReference type="CTD" id="7001"/>
    <dbReference type="OrthoDB" id="185659at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045321">
        <property type="term" value="P:leukocyte activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF111">
        <property type="entry name" value="PEROXIREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="6"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="10">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928980.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="21987" checksum="D6C05B4BEAAA9043" modified="2020-10-07" version="1">MASGKAYIGKPVPDFRATAVVDGNIKDIKLSDYRGKYVVLFFYPLDFTFVCPTEIIAFNDRVAEFRKINCEVIAASVDSQFTHLAWTNTARKDGGLGSTNIPLVADINHNICKDFGVLKEEDGIAYRGLFIIDDKGILRQITVNDLPVGRSVDETLRLVQAFQFTDQHGEVCPAGWQPGGDTIKPTVKDSKLFFAKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z072</accession>
    <name>A0A6I9Z072_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dual specificity protein phosphatase 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">DUSP16</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929684.1">
        <property type="nucleotide sequence ID" value="XM_014074209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z072"/>
    <dbReference type="GeneID" id="106555373"/>
    <dbReference type="KEGG" id="tsr:106555373"/>
    <dbReference type="CTD" id="80824"/>
    <dbReference type="OrthoDB" id="165342at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14646">
        <property type="entry name" value="DSP_DUSP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000016">
        <property type="entry name" value="Dual specificity phosphatase 16 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000050">
        <property type="entry name" value="Dual specificity phosphatase 16 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF343">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="7">
        <location>
            <begin position="23"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="159"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="228"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="324"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="443"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="594"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="455"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013929684.1"/>
        </source>
    </evidence>
    <sequence length="660" mass="72681" checksum="71D5908863ACBF27" modified="2020-10-07" version="1">MADEMNDAQLITGERLVTWLESSMEKLLLIDSRPFVEYNMSHILEAINIHCSKLMKRRLQQDKVLITELIQHSAKQKVEIDGKQEVVVYDQNSTDLATVPSDSFLSLLLRKLEKSFSNVHLLAGGFTEFSNCFPGLCEGKSILVPSCISQPCLPVSNTGPTRILPHLYLGCQRDVLNKELMQQNEIGYVLNASNTCPKPDFIPESHFLRVPVNDSFCEKIFPWLDKSVDFIEKAKASNGCVLVHCLAGISRSATIAIAYIMKRMDMSLDEAYRFVKEKRPTISPNFNFLGQLLDFEKKIKNQGGTSGLVSKLKLLHLENGSNHGAGGGVLRGPSQLCASSTSENMEQKPREPGTPPAPPLSPSAEGGSLVQSRTGLNVSSDKVEDSNRLKRSFSLDIKSVSCAAGNACLTASGPGYPSLEDTLEFYKHSATLEAGSKLCQFSPVQEVSEQTPEGSPDKEHTKLPEKSHQGWPPDSQNKRQHSGRATCPAQRSSLYPLHRSGSMEDNYRTNFLFGLSASQQHLAKSAAGLGLKGWHSDILSPQTSAASLTGGWYLTADPSHIYSASAVYGRSATYSAFSCSQLPTGCEPTYGVRRREKHGERGDSRRSWHEESSFEKQFKRRSCQMEFGGSIMSENRSREELGKMGSQSSFSGSMEIIEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1Q9</accession>
    <name>A0A6I9Z1Q9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Semaphorin-3F-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106555825</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930214.1">
        <property type="nucleotide sequence ID" value="XM_014074739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1Q9"/>
    <dbReference type="GeneID" id="106555825"/>
    <dbReference type="KEGG" id="tsr:106555825"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF28">
        <property type="entry name" value="SEMA DOMAIN, IMMUNOGLOBULIN DOMAIN (IG), SHORT BASIC DOMAIN, SECRETED, (SEMAPHORIN) 3GA ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sema" evidence="3">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930214.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="19424" checksum="478A7A75002FB3C7" modified="2020-10-07" version="1">MKAALELCMQTLKMHLLQGARLVCFLPPGDVFLLPTQDEKHPLLYGVFSTLGSVFRGSAVCVYSMADIRTVFNGPFAHKEGHNYQWGPYTGRVPYPRPGACPGGTFTPGLRSTREFSDELVTFVRAHPLMFHAVYPLQRRPLLVRTNVPYSFTTLAVDLVDAVDGRYEVLFLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1R4</accession>
    <name>A0A6I9Z1R4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 262</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMEM262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930219.1">
        <property type="nucleotide sequence ID" value="XM_014074744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1R4"/>
    <dbReference type="GeneID" id="106555832"/>
    <dbReference type="KEGG" id="tsr:106555832"/>
    <dbReference type="CTD" id="100130348"/>
    <dbReference type="OrthoDB" id="9406895at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040431">
        <property type="entry name" value="TM262"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37998:SF1">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED AUXILIARY SUBUNIT TMEM262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37998">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930219.1"/>
        </source>
    </evidence>
    <sequence length="117" mass="13536" checksum="03C5F925042904F8" modified="2020-10-07" version="1">MGSWKDPFLTVTFPSKVIFIISSWVLFVINLGVIIGDLYHFLVSQRGDLMSFHFTVSLLFSHVTSFYLALIASVYTMQADDGFLTCLSLTSLVANFSLYLARFWMDYLTIEYREEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z264</accession>
    <name>A0A6I9Z264_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 678-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106555914</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930324.1">
        <property type="nucleotide sequence ID" value="XM_014074849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z264"/>
    <dbReference type="GeneID" id="106555914"/>
    <dbReference type="KEGG" id="tsr:106555914"/>
    <dbReference type="OrthoDB" id="9031574at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004135">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000508">
        <property type="entry name" value="Myeloid zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001997">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001188">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002159">
        <property type="entry name" value="Zinc finger protein 841"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="14">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="151"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="501"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="585"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="613"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="641"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="669"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="697"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="725"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013930324.1"/>
        </source>
    </evidence>
    <sequence length="785" mass="90248" checksum="6BADFE121996FA7D" modified="2020-10-07" version="1">MCSHLHQLCRQWLQPEKNTKGQMLDLVILEQFLAILPSEMESWVRECEAETSSQAVALAEAFLLSQMEEKKEKAEGQALHFLRPDLKSISVNPEGKRDIPDLSQELVFSAIFHEDQSQDTSCENGKSAFIGSSPFSDGVEKTVEPPTQILVSLEEVAVCFSEEEWSRLDPVQKDLHREVMLENSRNVAFLGNNLQENKEAVQMFRDEERRKAFSYPMKTTENERNLSNNGSKRSPTIEIQDFLLKGDQKENLKVENVEPFENLDLYEHYTIHIKEEDLGQEVEKSYNWTSNISLCGEAKMRNKGYKSTENEKHLSGSSSLVPSERTVSGGKPYKCMECGKSFSQRGNLNSHKKIHTEEKPYKCDECGKSFTQKVNLNSHKMIHTGEKPYKCEECGKSFTQNIQLTSHQRIHTGEKPYTCMECGKTFRWSTQLTSHQRIHTGERPYKCMECGKTFSESSSLTFHKRIHTGEKPYNCMECGKSFSMSSSLTYHKRIHTGEKPYKCLECGKSFTQNIQLTSHKMIHTGEKPYKCTECGKNFNTTSNLACHKRIHTGEKPYKCIQCGKSFTQNIQLTSHVRIHTGEKPYECKECGKSFTQKIQLTSHKRIHTGEKPYKCRRCGKSFRNNGSLTLHERIHTGEKPYTCIVCGKSFSRGSHFASHKTIHTGEKPHKCLECGKSFRENTSLTIHKRIHTGEKPYKCLECGKRFRENGSFTSHKRIHTGERPYKCMECGKSFTTSSNLTSHMRIHLVKDMQENENKRKSSMLTSRIMKHEMREERFGNHGESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2U6</accession>
    <name>A0A6I9Z2U6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Treacle protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TCOF1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931398.1">
        <property type="nucleotide sequence ID" value="XM_014075923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2U6"/>
    <dbReference type="GeneID" id="106556883"/>
    <dbReference type="KEGG" id="tsr:106556883"/>
    <dbReference type="CTD" id="6949"/>
    <dbReference type="OrthoDB" id="9838304at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097110">
        <property type="term" value="F:scaffold protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017859">
        <property type="entry name" value="Treacle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20787">
        <property type="entry name" value="TREACLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20787:SF10">
        <property type="entry name" value="TREACLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="691"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="761"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="784"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="800"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="875"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="889"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1016"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931398.1"/>
        </source>
    </evidence>
    <sequence length="1032" mass="106727" checksum="92548A22012BACE3" modified="2020-10-07" version="1">MATTPREQKELLALIHQQLVQMGYVSAAKELLEQSGQKSLPSSHVSLKEIITQWKQTYSQIPKQKAIETKRGTPGKLRIPDPKSSSESSEEEEATQTIKTNLRGNNSTKPTVAKEKSSSEDSSSDSSSEEEAVAKKTEKTPVAGKKPELLKLAVQNTNSVPGKGVTVAAAQAKGEQNKSVSNRALPPAARKAGPNKALSSKAGTPSQSLISGGQKTTQTTKTAESSESSSSTSSESEEETTLTTVKAPPSKPTLKRARSTSKGSSDDSDSEDETKAPTVQIKTAVKNSPITSPLLSKSPVTPGTCARNTVKVTAAKPASAKSGNANRSPESTDTSETDSSASETKAPASASQASKAPGKNAASSPSKKAVSASIPSKSTPKATATHLSKPPPPSKEAESSGSSESSSESDSETPPVPISQKASTAPGKNAASSPSKKAVSASIPSKSTPKAPATRLSKPPPPSKKAESSGSSESSSESDSETPPVPISQKRLSTPSASVRTKQNQANQPGVLPKASPGALKEKEAQNESSSSSDSEDESDVTPSKLPALSTPKATPAAQSVCTVKPQPPKGPHQKAEQSEDSSEDSSDDSDSEEDGAATQKPAQVTGQPATSPVKSMTAKNAGLTSSKPQPAGKAGTTSTKPGTVPVSKASESSESSSSDSSDGKEAEKSAKQPPIHPFFKLVAGKVAVSSGKSVQANLSTSSAVSDKPQGSATKATGKKEQIPSSKVKLSQGQALTAKPSGITVPESSSDSSEEEEEEASTTAKPPTQPATQQKQEAKKESESSSEESSDDEQEASQSLLTASPLVSKQGSVSAQAKSSGMPAPADRASSTDSSSSDSDDGETLTAKKEITSLPSSGKEATAAKGKEAKKQGLKSTSSKKAAAKAQSNGQAQETSAVQLPESLAALFKAPLGNKEKDTEQGNPEGTQAKSKTSKNVKKEKPSKKRKLTDGDTGPSKGKKRKMQSAEELKLKKKKKKAKSSDSIKSTKKKDSKEKKSDKKKKKSKNVERLDADGSQKKKKKKKKTDSLPEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z333</accession>
    <name>A0A6I9Z333_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106555867</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013930270.1">
        <property type="nucleotide sequence ID" value="XM_014074795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z333"/>
    <dbReference type="GeneID" id="106555867"/>
    <dbReference type="KEGG" id="tsr:106555867"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14209">
        <property type="entry name" value="STKc_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044109">
        <property type="entry name" value="STKc_PKA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF116">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0114">cAMP</keyword>
    <keyword evidence="7 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="34"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="289"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013930270.1"/>
        </source>
    </evidence>
    <sequence length="341" mass="39770" checksum="E2D6EAC914BC5044" modified="2020-10-07" version="1">MNEIFVKEFLAKAKEDFLRKWESPAQNTAGLDDFERLKTLGTGSFGRVMLVKHKSTEQYYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVRLEYSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRYGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKCRGPGDTSNFDDYEEEDIRVSLTEKCAKEFADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3J0</accession>
    <name>A0A6I9Z3J0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ubiquilin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106556757</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931217.1">
        <property type="nucleotide sequence ID" value="XM_014075742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3J0"/>
    <dbReference type="GeneID" id="106556757"/>
    <dbReference type="KEGG" id="tsr:106556757"/>
    <dbReference type="OrthoDB" id="9450922at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF16">
        <property type="entry name" value="UBIQUILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23195">
        <property type="entry name" value="UBQLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="25"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931217.1"/>
        </source>
    </evidence>
    <sequence length="450" mass="48562" checksum="B1D87D3E382EE558" modified="2020-10-07" version="1">MEAMNMHQEGAPHGCPSLASNPNFVCVTAKSAQGRAQFALPRETTIRQFKEGLSKHFQCERRQLVLVFFGRILKDQCTLQQCGISDGMTVHLVIRSPKGGPEDPTQSSYMPRTPSCTLAPTGTSLTLPALKPTCHGSWGPPEWHQMPTSEMVVQKVRQLILANPEIQQLAQQFPAICHILNNMGIMRVILDKMREIMDLAKNSDVEQDLKNPEPLLLSVQSNTPGGDNPLRQLNSEVQEPGLNAGQGPLATPSPYVTLARNPSAEHKARDGEGGGSLCSDSFPISNSYGRFCTNNSSNEEGGLFNSLPGLLGSSLPSLGPNPGAGAGNVGGVQALIVNLCNAYTQRAMLSLMQNALLANEGTQGPHQDHVQQQLQSFYRQMQRPEMVAALSSPKAIQAWVQMEQGLQMLREEAPVLLPWFLLHMKGLGLTSAPGALAAGSPSEELPAVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z469</accession>
    <name>A0A6I9Z469_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tumor necrosis factor receptor superfamily member 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106556563</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931019.1">
        <property type="nucleotide sequence ID" value="XM_014075544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z469"/>
    <dbReference type="GeneID" id="106556563"/>
    <dbReference type="KEGG" id="tsr:106556563"/>
    <dbReference type="OrthoDB" id="9423210at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47139">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47139:SF1">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026966473" evidence="3">
        <location>
            <begin position="19"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="4">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013931019.1"/>
        </source>
    </evidence>
    <sequence length="235" mass="26226" checksum="88F4F0BCAF439CB4" modified="2020-10-07" version="1">MERLFCALLLLLCTGAVCEVPCPKGAFRPGKGQSCQQCRVCDRTLVYKQKCTETSDARCRCAEGYSCGKNCEACECPVGQQRTITGCQKCTPKTFNNRTTGECRPWSTCPGDRIREPGTEKRDVVCWSGSEIPTTQPSTSLAVFSTTVSEWGNDLILVTSAFIIIIALCLAFMVLCYFFLRYWLRKKFPKPSHGQLAQEVDDCSYRYPEEEEGGSCEALSSLKGGLLERYYTQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z715</accession>
    <name>A0A6I9Z715_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone-lysine N-methyltransferase SETMAR</fullName>
            <ecNumber evidence="12">2.1.1.357</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106557036</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl(36)-[histone H3] + 2 S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60308"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.357"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.357" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013931620.1">
        <property type="nucleotide sequence ID" value="XM_014076145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z715"/>
    <dbReference type="GeneID" id="106557036"/>
    <dbReference type="KEGG" id="tsr:106557036"/>
    <dbReference type="OrthoDB" id="616263at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140954">
        <property type="term" value="F:histone H3K36 dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10544">
        <property type="entry name" value="SET_SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000041">
        <property type="entry name" value="Histone-lysine N-methyltransferase SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050973">
        <property type="entry name" value="H3K9_Histone-Lys_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pre-SET" evidence="15">
        <location>
            <begin position="46"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="14">
        <location>
            <begin position="111"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="16">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013931620.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="32007" checksum="ACAFC58DD27C257E" modified="2020-10-07" version="1">MDVSRGLEPLPVSVWPRLEELPVFQYSPDHVPGAGGKPDPSEISFSGCSCHSASCLAPACSCLHYGENYKNLSMETEGSELGFSKPIFECNAMCHCGEMCQNRVVQRGLQFQLEVFKTTEKGWGLHTREFIPKGRFVCEYAGEILGIDEARKRTQSQTSSDSNYIIAIREHLYDGTTLETFVDPTYIGNVGRFLNHSCEPNLFMVPVRIDSMVPKLALFADRDIGAGEELSYDYSGRYHNFFPIEEQGSKQEQQVSKKPCFCRTKSCTGFLPFDSSLFLKDGKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z8K1</accession>
    <name>A0A6I9Z8K1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 21 22" type="primary">SEPT2</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932097.1">
        <property type="nucleotide sequence ID" value="XM_014076622.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932105.1">
        <property type="nucleotide sequence ID" value="XM_014076630.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932112.1">
        <property type="nucleotide sequence ID" value="XM_014076637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z8K1"/>
    <dbReference type="GeneID" id="106557386"/>
    <dbReference type="KEGG" id="tsr:106557386"/>
    <dbReference type="CTD" id="4735"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000064">
        <property type="entry name" value="Septin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008113">
        <property type="entry name" value="Septin2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01740">
        <property type="entry name" value="SEPTIN2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 16 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="18">
        <location>
            <begin position="36"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="16">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006698-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013932097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013932105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013932112.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="41974" checksum="0F80D197506DE6CE" modified="2020-10-07" version="1">MSKQQQPAEKFANPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERIIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFRTIISYIDEQFERYLHDESGLNRRHIVDNRVHCCFYFISPFGHGLKPLDVEFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHSIKIYHLPDAESDEDEDFKEQTRLLKTSIPFCVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKKGGRKVEDEEVNKDQILLEKEAELRRMQEMIARMQAQMQMQRQGGEGDSSATHGYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WX11</accession>
    <name>A0A6I9WX11_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein FAM13C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FAM13C</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907464.1">
        <property type="nucleotide sequence ID" value="XM_014051989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WX11"/>
    <dbReference type="GeneID" id="106537760"/>
    <dbReference type="KEGG" id="tsr:106537760"/>
    <dbReference type="CTD" id="220965"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039102">
        <property type="entry name" value="FAM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059029">
        <property type="entry name" value="FAM13A_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904">
        <property type="entry name" value="FAM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904:SF19">
        <property type="entry name" value="PROTEIN FAM13C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26116">
        <property type="entry name" value="FAM13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM13A-like" evidence="4">
        <location>
            <begin position="423"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="208"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013907464.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="57117" checksum="7AD0772BFF9187D0" modified="2020-10-07" version="1">MRTASKKDLSKFGETIFYLMSAKVLRSLEMGNAGSWLSECQKKTTTSATEYAENVTLRYQETALKFQPRTDQSAFISSNDAAEHLQRLTEEDSPQLVASEEPASSSASTSMHIDRDYGGELAQGYQDGQMEAENGPEDQDALGTCRLLHHITDGDYPLLSPRCSIFSQSQRFNLDPESAPSPPSAQLFMMPRSSSRGSCEDSKVPQTMVQLTKHLQNLKRRIRKYEEKFEEEKHYRPSHADKISNPEVMKWMNDLAKSRKQLKELKLKMTEEQSFPIRKNQRSFYPESPREAGMLAPADSPTAPSVEETMEVVKKQLKEKRQQLGLPENIKVNQQDRSLMKPLYERYRIIKQLLSAPSLITTIEEEDSDEEPQSSSTRYLYGPLQAQMDHSDEENEPALVSPRGEKKVMKQPALMSNLHEATMPVLLDHLRETRADKKRIRKVLREFEEEFFRQTGRIPQKEDRISVSEEYLQYKHIKAKLRLLEVLISKHDVPKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZ29</accession>
    <name>A0A6I9WZ29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LysM and putative peptidoglycan-binding domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LYSMD2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908214.1">
        <property type="nucleotide sequence ID" value="XM_014052739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZ29"/>
    <dbReference type="GeneID" id="106538292"/>
    <dbReference type="KEGG" id="tsr:106538292"/>
    <dbReference type="CTD" id="256586"/>
    <dbReference type="OrthoDB" id="2107166at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF4">
        <property type="entry name" value="AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="57"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013908214.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="22380" checksum="355A072B04C9BBBE" modified="2020-10-07" version="1">MAESLLPVPGLSLREEPLPGPGAAEGEAELSLSLARTKTRSYGSTATVVAPLAERYVEHCLSPGDTLPGLALKYGVTMEQIKRANKLFTNDCIFLRKTLNIPVILEKALLFNGCNSLESPEDDIDNPTPCEESPMTVQEDNSSPSPQESDNRLPPPEELSAKDFLHRMDVQIKLSKQAAKKLKSEDNREYDEENSYAISSYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1B4</accession>
    <name>A0A6I9X1B4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">28S ribosomal protein S11, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106538927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013909044.1">
        <property type="nucleotide sequence ID" value="XM_014053569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1B4"/>
    <dbReference type="GeneID" id="106538927"/>
    <dbReference type="KEGG" id="tsr:106538927"/>
    <dbReference type="OrthoDB" id="1654884at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013909044.1"/>
        </source>
    </evidence>
    <sequence length="106" mass="11640" checksum="85349A16BF15FFEC" modified="2020-10-07" version="1">MLAALAIARRGLLGGGSGITFSRSIQTGVQRFQTLLQNTDSATDKELDSTQSKGFSYFPPVLGEESSLRWDGKKFEEIPIVHIKATYNNTHIQVFTAEKVPVVLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2T0</accession>
    <name>A0A6I9X2T0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tumor protein p53-inducible nuclear protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TP53INP2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
            <location evidence="10">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908631.1">
        <property type="nucleotide sequence ID" value="XM_014053156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2T0"/>
    <dbReference type="GeneID" id="106538623"/>
    <dbReference type="KEGG" id="tsr:106538623"/>
    <dbReference type="CTD" id="58476"/>
    <dbReference type="OrthoDB" id="10041339at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029431">
        <property type="entry name" value="TP53INP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671">
        <property type="entry name" value="DIABETES AND OBESITY REGULATED, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671:SF2">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE NUCLEAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14839">
        <property type="entry name" value="DOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013908631.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="27342" checksum="870E268CE4350CB4" modified="2020-10-07" version="1">MFQRFTSLFFSDSSAVEVVEEPKPFVSKEEEEDGWLIIDLPDSLASSLGTEQWVGEKDSVCASRCRHGSLPPTTSSPHSLSDCVSRSMPSQPDPCLMDESWFVTPPPCFTAEGPDPVSMESSPMEDLLIEHPSMSVYVTTTTSIIVEREDPEENIQENEIPENCLQRRPPHHSTSLATKAAILEKVNQVRRIQRAKQLAEKHKLSQKVMKRQNRARECRPQRAKHQGHFVYQPCQRQYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2X4</accession>
    <name>A0A6I9X2X4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">cathepsin E</fullName>
            <ecNumber evidence="5">3.4.23.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CTSE</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Similar to cathepsin D, but slightly broader specificity.</text>
            <dbReference type="EC" id="3.4.23.34"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.34" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013909634.1">
        <property type="nucleotide sequence ID" value="XM_014054159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2X4"/>
    <dbReference type="GeneID" id="106539374"/>
    <dbReference type="KEGG" id="tsr:106539374"/>
    <dbReference type="CTD" id="1510"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019886">
        <property type="term" value="P:antigen processing and presentation of exogenous peptide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000006">
        <property type="entry name" value="Cathepsin E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000004">
        <property type="entry name" value="Pepsin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF26">
        <property type="entry name" value="CATHEPSIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07966">
        <property type="entry name" value="A1_Propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027028033" description="cathepsin E" evidence="17">
        <location>
            <begin position="17"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="18">
        <location>
            <begin position="74"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="105"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="268"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013909634.1"/>
        </source>
    </evidence>
    <sequence length="395" mass="43981" checksum="FCB9C55751893E67" modified="2020-10-07" version="1">MNWLIFLTFYIWMTSGLQRVTLKRHRSLRQILRERGQLTQFWKKYKIDMIQYTQDCSVFKETNEPLFNYFDVEYFGQISIGNPPQNFTILFDTGSSNLWVPSIYCVSKACVEHSRFHPSQSSTYTEVGTSFSIHYGTGSLTGIIGMDQVTVEGLTIVNQQFAESVSEPGNTFLDSEFDGILGLAYPSLAVDGVTPVFDNMMAQNLVDLPIFSVYMNRNPYSSVGGELIFGGYDEAYFSGNLNWIPVTKQGYWQIQLDNIQVGGNIAFCADGCQAIVDTGTSLITGPSKDIKQMQKLIGAQAMDDEYAVECNNLNVMPLVTFTINGNAYPLTPEAYTVMVNSDGINLCTSGFQGLDIESATDPLWILGDVFIGQYYSVFDRGNNRVGLAPVVSQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3Y8</accession>
    <name>A0A6I9X3Y8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Laminin subunit beta-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LAMB3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910019.1">
        <property type="nucleotide sequence ID" value="XM_014054544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3Y8"/>
    <dbReference type="GeneID" id="106539665"/>
    <dbReference type="KEGG" id="tsr:106539665"/>
    <dbReference type="CTD" id="3914"/>
    <dbReference type="OrthoDB" id="8545473at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043256">
        <property type="term" value="C:laminin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070831">
        <property type="term" value="P:basement membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000084">
        <property type="entry name" value="Laminin subunit alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000440">
        <property type="entry name" value="Laminin subunit beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000073">
        <property type="entry name" value="Laminin subunit beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000001">
        <property type="entry name" value="Laminin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000333">
        <property type="entry name" value="netrin-4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000224">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056558">
        <property type="entry name" value="LAMB1-4_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF268">
        <property type="entry name" value="LAMININ SUBUNIT BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23219">
        <property type="entry name" value="LAMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026801148" evidence="14">
        <location>
            <begin position="17"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="16">
        <location>
            <begin position="21"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="249"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="313"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="376"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="428"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="478"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="531"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="832"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="945"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="480"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="533"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="564"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013910019.1"/>
        </source>
    </evidence>
    <sequence length="1166" mass="129564" checksum="65D4FEC27D6913F0" modified="2020-10-07" version="1">MMWIFLLLASSHLLVAQRTCLHGACYPPVQDLLIGRIHYLKASSTCGLTQPETSCTPYGKWQMKCCSCDSRTPHAYNSHRVENVLSSTGHKRWWQSQNDVKHVSLKLDLNNKFHLASLLLDFRSPLPGGMVIERSTDFGKTWKIYQYLATDCSTTFPRIPKGLPQKWQDVHCQNIQSQHGQPQNGGKIKFNPVDLASGMMTSQNQRINNLAEFTNLRINFTQLTHFPHQGYRLPSAFYALNEMQVQGSCFCHGHADRCAAPDASSAEPRAMVHGRCICQHNTAGPNCDHCASFYNDQPWKPAEDDNPNECHRCNCNGHSHKCHFDQSVYQANGGKSGGVCEDCQHNTEGRNCELCKINFFRNRQFDLGHPEVCLPCECDPDGTVPDSSCDRMNGRCVCKDNVQGDRCHLCKPGFTQLTNSNPKGCRECACNMLGSRRNVPCDDETGRCFCLPNVAGEKCDECAVNHWKIASGEGCWPCNCHPQNSLSLQCNKFTGQCPCREGYGGLTCSAVEVQECPHQTFGNVRTGCRDCDCNFQGTEVMGCNKLTGSCLCRSGFAGPQCNQCQRGYCGNYHQCDACHPCFQAYDDDIQRFGLRQASLKNSTQHLQGGTMTSGYSTRLLEMERKMQHIQQMLDNPLITEQGLDQVANAIATIRQTIEGLSSEIFLVDEVSSLSIDMDALDKSLYLITKQYQNKKAQFEASRSIDLSGAFKTIRSAHVTSSNASARIAGSSVLLAESLEKRRNAEELESRLLDPDSGFELLQNKMAVAPNLTPVINKVCSVVRSEVCTPGECIGYLCSHQNANECGPGMSCRGILALSGGSIQTARKTAQEFRNFNSQLQETEQMIRAAEIASSKIQTNAQKLRNQMSIIRTQIEADIQRTQQFIRQVRNFLSDPDADATTIQQVSNYILSLNLPIDTGTILRKITEIQNLIAKLQCPDSIIAQTAGDIAKAKQLQQEAEEARNQASTIEGNVDDVLQILKQGEIALQEAELTIQDSAFPLQSIQNRLEEIQTILTPAQENVRDITNQLDSFTETLVQLQHKASQNQLLAADAQQQATEAHAQAKTTQQVFEQLNGKYMDLQRRMGQGSNLGIQGDRIQSAHTEANTLLNEALNMMAKMSALETELQESNSAFILKSAKLEGLEKKVESIRDHISQRVAYYSSCTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4X1</accession>
    <name>A0A6I9X4X1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRKCA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein interacting with C kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Protein kinase C-alpha-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28 29 30" type="primary">PICK1</name>
    </gene>
    <organism evidence="27 30">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28 29 30">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Monomer and homodimer. Interacts with CXADR. Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2. Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ASIC1 and ASIC2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR. Interacts with UNC5A. Interacts (via AH domain) with NCS1/FREQ; in a calcium-dependent manner. Interacts with F-actin and associates with the ARP2/3 complex. Interacts (via PDZ domain) with ARF1 (activated); the interaction blocks Arp2/3 complex inhibition. Interacts with SORCS3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910432.1">
        <property type="nucleotide sequence ID" value="XM_014054957.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910433.1">
        <property type="nucleotide sequence ID" value="XM_014054958.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910434.1">
        <property type="nucleotide sequence ID" value="XM_014054959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4X1"/>
    <dbReference type="GeneID" id="106539995"/>
    <dbReference type="KEGG" id="tsr:106539995"/>
    <dbReference type="CTD" id="9463"/>
    <dbReference type="OrthoDB" id="5917245at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoskeleton"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098842">
        <property type="term" value="C:postsynaptic early endosome"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein kinase C binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0097062">
        <property type="term" value="P:dendritic spine maintenance"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0002092">
        <property type="term" value="P:positive regulation of receptor internalization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:receptor clustering"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07659">
        <property type="entry name" value="BAR_PICK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06722">
        <property type="entry name" value="PDZ_PICK1-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Interacting with PRKCA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interacting with PRKCA"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
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    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
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    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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    <dbReference type="InterPro" id="IPR037959">
        <property type="entry name" value="PICK1_BAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF1">
        <property type="entry name" value="PRKCA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="25">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="AH" evidence="26">
        <location>
            <begin position="144"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022599"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023018"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023139"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00031097"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013910432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013910433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013910434.1"/>
        </source>
    </evidence>
    <sequence length="410" mass="46195" checksum="9059243FD92D44CE" modified="2020-10-07" version="1">MFADLEYDIEEDKLGIPTVPGTVTLKKDSQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTLAAGDEITGVNGKSVKGKTKVEVAKMIQIVKGEVTIHYNKLQADPKQGKTLDIVLKKVKHRLVENMSSGTADALGLSRAILCNDGLVKRLEELERTAELYKGMTEHTKSLLRAFFELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIHLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIAMGEPLYRVGTGNYEYRLILRCRQEARSRFAKMRKDVLEKMELLDQKHVQDIVFQLQRFVSTLSNYYDDCYAVLREADVFPIEVDLARTTLSYGQKDVFTDGADDEEEEEGCGSRECNKKEEENGEKLIDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X758</accession>
    <name>A0A6I9X758_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA endonuclease RBBP8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25 26 27 28 29 30 31" type="primary">RBBP8</name>
    </gene>
    <organism evidence="24 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26 27" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25 26 27">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COM1/SAE2/CtIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910073.1">
        <property type="nucleotide sequence ID" value="XM_014054598.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910082.1">
        <property type="nucleotide sequence ID" value="XM_014054607.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910091.1">
        <property type="nucleotide sequence ID" value="XM_014054616.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910099.1">
        <property type="nucleotide sequence ID" value="XM_014054624.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910108.1">
        <property type="nucleotide sequence ID" value="XM_014054633.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910115.1">
        <property type="nucleotide sequence ID" value="XM_014054640.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910123.1">
        <property type="nucleotide sequence ID" value="XM_014054648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X758"/>
    <dbReference type="GeneID" id="106539733"/>
    <dbReference type="KEGG" id="tsr:106539733"/>
    <dbReference type="CTD" id="5932"/>
    <dbReference type="OrthoDB" id="5801062at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010792">
        <property type="term" value="P:DNA double-strand break processing involved in repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019518">
        <property type="entry name" value="CtIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013882">
        <property type="entry name" value="Ctp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033316">
        <property type="entry name" value="RBBP8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107:SF4">
        <property type="entry name" value="DNA ENDONUCLEASE RBBP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107">
        <property type="entry name" value="RETINOBLASTOMA BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10482">
        <property type="entry name" value="CtIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08573">
        <property type="entry name" value="SAE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 25 26" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0469">Meiosis</keyword>
    <keyword evidence="11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DNA endonuclease Ctp1 N-terminal" evidence="23">
        <location>
            <begin position="21"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="DNA endonuclease activator Ctp1 C-terminal" evidence="22">
        <location>
            <begin position="783"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="698"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="64"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="702"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF10482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013910073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013910082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013910091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013910099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013910108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013910115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013910123.1"/>
        </source>
    </evidence>
    <sequence length="854" mass="97830" checksum="39DA11DDECD90832" modified="2020-10-07" version="1">MSASGGNCGSPNSTEPASDCFKELWSKLKECHDKDVKGLLSKISKLKAERCLDAARLDEFYTKNQQLREQQKSLHDTITVLEDRLRAGLCDRCAVTEEHMKKKQQEFENIRQQNLKLITELINERNNLQDENKKILEQLQKMQQKVEEQEQYMEDVIPGSPVQSLSLSMVNRMRRKRENRHVRYTEQKHTDLEKKGRIYELGNITLSSTQKPAWSEEEILVADTCDPQLSPDSSNEVKEECPNSVHFNLKAIVAETLECDEHEPRSKSLEVTRKQLAQGCFKSNTDNLEFSDRSEDISPTTWDSKIPSPVFGASANIKSSLVFSPSVLAAEKKSNQSSSINSSSLSRSEKVRSKSEDIRFMSHGTEIATVLSQAPVGRQVALKQNSEEAITCVTNTHLDKSDIIKGGFLVSQKRLGNRCSKRKKNDDKSNHVIRCEKENAFPSPADVLPIARDNTTDKPLDLSDRFSDFPCEKIIAESSKIKMKQATLHDVFQPLVKDSTSKITTSNSVMNKDSQEHPCIQEAILKSLTKMSPVKEHEVKEEIANLSNPSLQNDDSIEEAAGDHELIRKRIRSGHGIGEPASVLQPNPRKVTKITQFEQDKTSLENVQWSIDPGADLSQYKMDITLIDTKEETQTRIGSEPLDMDHTYISDSILLRMKKQRQKEVSNPEGEKREKDTLTEIFDQTTYEEYKSYLEDDNLPECDYKNSSEEEGKEKSTIQRERDKQNKMKQKAFVETYFKSDERTNLVLDFPHIEVVRKKEERRKLPGHSCKECEIYYAGLPEEEREKKLATCSRHRSRYIPPETPENFWEVGFPSTQMCVERGYIKEEFAPCPRPKRRQPYNVMFSPKGKEQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7E2</accession>
    <name>A0A6I9X7E2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch domain-containing protein 7A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KLHDC7A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909757.1">
        <property type="nucleotide sequence ID" value="XM_014054282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7E2"/>
    <dbReference type="GeneID" id="106539483"/>
    <dbReference type="KEGG" id="tsr:106539483"/>
    <dbReference type="CTD" id="127707"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052310">
        <property type="entry name" value="Kelch/BTB_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45972">
        <property type="entry name" value="BTB_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45972:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013909757.1"/>
        </source>
    </evidence>
    <sequence length="847" mass="92979" checksum="5962F4192D78A988" modified="2020-10-07" version="1">MSHRAEGSGDQPSEIPLAGRLALSAAALLLLMLAYRYFKSRVPPGSTPAGAADANVAGAEQREWQVGSQVTNKPAALRHRGGRGKETEPCHPGTNQLTQVGRCDGVVAEECGQAKIPAGRGLPGTRETEAEEDQEAGEVSHAESGVEHRWRSEGTIQNCTSKAGADLDNFGGHSRAVGLLNREKEAGNIPGWDVDPAEADSSCAKPVEKEVHPADGSDIGLKRGDPPQLGKESKALHVVERPLGREKIRSFYATSDTGLAINHNHVQSQVAYAFSSVAKVEVEENFIKEKQGSGKAEDAQLRGKIYNYYVQSASQSVTNKTTFTTNSDLQQSCNLSTSNILNVSKKCGAEEPVHSSVTNNDVNKLVLEKAPAPSSNVLLSPEKDTQPKDQREAVVVEEAISSKPPLARYSFIRKDSFQRIVDNPDLQMPMEGFGSPAMGPSAVLPSSPAPLCLDSTSSLVRSMQDLPSSGTEEPLVELVAGAKFFHVPMSSESFLDVHLDLGNCYQVLCMAKKQKLKDLQEAAYKVMSDNYLQVLKTQGIYRQLNAAERDLILEKRMRGKKYLTVADIGCHPSGLSYYDDQKDAWHPLTHIPVEAISRGCAICSMFNYLFVVAGCEGRGRYQKPSNRVFCYNPVTDIWQEICPLNQARPHCKLVALDGCLYAIGGECLYTVERYDPRLDRWAFAAPLPNDTFAVAHTATVCDGEIYVTGGTLRYMLLRYVGRTDTWKVRLTGGSQDRTTEMVTVNGFIYRFDLNQRMGISVYRCSARAKLWYECATHPMPFPPCFQCSVVGNLVYCVSRQFNLRFLADLVFPRFGLKELKNFPSPRGTLIPVTLILPEGDKAPQTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7S3</accession>
    <name>A0A6I9X7S3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22 23" type="primary">KDM4C</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013911533.1">
        <property type="nucleotide sequence ID" value="XM_014056058.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911534.1">
        <property type="nucleotide sequence ID" value="XM_014056059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7S3"/>
    <dbReference type="GeneID" id="106540806"/>
    <dbReference type="KEGG" id="tsr:106540806"/>
    <dbReference type="CTD" id="23081"/>
    <dbReference type="OrthoDB" id="9547406at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15715">
        <property type="entry name" value="ePHD_JMJD2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20465">
        <property type="entry name" value="Tudor_JMJD2C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000048">
        <property type="entry name" value="Lysine-specific demethylase 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000029">
        <property type="entry name" value="lysine-specific demethylase 4C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.70:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040477">
        <property type="entry name" value="KDM4-like_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF104">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18104">
        <property type="entry name" value="Tudor_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="18">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="19">
        <location>
            <begin position="144"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="749"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="366"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="506"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013911533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013911534.1"/>
        </source>
    </evidence>
    <sequence length="1053" mass="120441" checksum="0733F72A71F3375E" modified="2020-10-07" version="1">MAAQSLDSPPNPSCKIMTFQPTMDEFKDFNKYLAYMESKGAHRAGLAKVIPPKEWKPRKSYDDIDDLMIPAPIQQMVTGQSGLFTQYNIQKKPMTVKEFRQLANSDKYCTPRHLDFEDLERKYWKNLTFVAPIYGADINGSIYNEGVKEWNIAHLNTILDIVEEDCGISIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWYAIPPEHGKRLERLAQGFFPSSSQGCDAFLRHKMTLISPSILKKYGIPFNKITQEAGEFMITFPYGYHAGYNHGFNCAESTNFATIRWIDYGKVAKLCTCRKDMVTISMDIFVRKFQPDRYQLWKQGKDLYNIDHTKPTPETTPEVKAWLQRRKKTKRCSSFQHTRSRSKKLKTPEDKKPSTIEVGTEITATEAATDCFKVKDKPSQKVKPGDTGVPSGEEQVSSRMHIDQNLLDNKKILGHSPSSSQLDQVPEMHNIESEKEEKEVSDLARSFSEESKVALLSYPDCNQDVLKTSNISRQTSENAASEMENFEKEEINSVSTEEDEISDAESNKSGLEPGEISTIFEGEKSKTSKSWRHPLNKPPARSPMTLVKQQAISDEELPEVPLIEEEVQETESWAKPLVYLWQNRIPNFTAEKEHNAAVAKLEPYCAICTLLKPYYKADKPNEEIYAWSETKQKEICMSNEKTKPLIPEICFIYSEENTENYPSNGFIEEDGTSILISCAKCCVQVHASCYGIPSHEIHDGWLCCRCKRGAWTAECCLCNLRGGALKQTTDKKWAHVMCAIAIPEVRFGNVTERTPIDTTRIPLQRLKLKCIFCRQRIKKISGACIQCSYGRCPASFHVTCAHAAGVLMEPDDWPYVVYITCFRHKISQNVKTKPFEKAVSVGQTVIAKHRNTRYYSCRVIEVTSQVFYEVMFDDGSFSRDTFPEDIVSRDCLKLGPPAEGEVVQVKWPDGKLYGAKYFGTNVAYMYNVEFEDGSQIATKREELYTLDEELPKRVRARFSTASDMRFEDTFYGADIIQGQKKRQRVLSSRFKNEYVDDPGYRSFLKASFQKKCQKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X848</accession>
    <name>A0A6I9X848_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protein spire homolog 2 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SPIRE2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the spire family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907503.1">
        <property type="nucleotide sequence ID" value="XM_014052028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X848"/>
    <dbReference type="GeneID" id="106537775"/>
    <dbReference type="CTD" id="84501"/>
    <dbReference type="OrthoDB" id="10043757at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051295">
        <property type="term" value="P:establishment of meiotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040038">
        <property type="term" value="P:polar body extrusion after meiotic divisions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22186">
        <property type="entry name" value="WH2_Spire1-2_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22065">
        <property type="entry name" value="WH2_Spire_1-2_r1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011019">
        <property type="entry name" value="KIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029901">
        <property type="entry name" value="Spire"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21345:SF5">
        <property type="entry name" value="PROTEIN SPIRE HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21345">
        <property type="entry name" value="SPIRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16474">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00750">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51377">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="KIND" evidence="15">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013907503.1"/>
        </source>
    </evidence>
    <sequence length="618" mass="70206" checksum="F74824F34559B0B7" modified="2020-10-07" version="1">MVQSLGFVIYRALDWGLEENEERELSPELERLIDLMTNGDSGDGGNGSSTADEGYSGQEEEEEAVAKGTLSVVRTFGQVMQLCAARLSQPQEAHAHYQAVCRALFLETVELKAFLAKIRDAKEMLVKLKDEELKDRSAAELDSLKNTDWARLWVQLMRELRNGVKLKKVQQKEFDPLPTEYQLTPFEMLMQDIRARNYKLRKVMVDRDILPRVKKDAHELILDFIRSRPPLRQASERRLRPLPQQQRTLHEKILEEIKQERKLRPVALQPRDQRGFGSLPCLLSACSSNMKSTSCINLSVSESGAPPQRQRPRVLLKAPTLAEMEEMFVSEEDDSPCSEAAQEPGPLMPLKRDRSFSEQDLAHFRDESEDSEPHLEYLRTSLSDPRPRSGSVPARYHPRVSSCPRKQEALGSVQENLEGNPVPVTDTSLKYLWLQEFSHPVESLALTVEEMINVRRVLVKAEMEKFLHSKELYTSLKRGKSCCCCRTKFTLFSWPPTCLFCKRSVCASCSLKIKMPSKKLAHIPVYALGFETLQGSLGAKGPPARRQETFHSLQGTRWRSVEEEFPCIYAHGAVLKDVCRDCSGFVTDVVSSSRKSMDILNTKPGRSRKTQSLYISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X865</accession>
    <name>A0A6I9X865_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase</fullName>
            <shortName evidence="19">POMGnT1</shortName>
            <ecNumber evidence="19">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">POMGNT1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;2)-alpha-D-mannosyl)-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54128"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:13802"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="ChEBI" id="CHEBI:138067"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="19">The manganese ion interacts primarily with the substrate UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with DAG1 (via O-linked mannose moiety). Interacts (via transmembrane domain) with FKTN; the interaction is direct and is required for normal location in Golgi membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Golgi apparatus membrane</location>
            <topology evidence="1 19">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="19">The stem domain mediates specific interaction with beta-linked N-acetylglucosamine moieties of O-glycosylated proteins. It also interacts with its product, N-acetyl-beta-D-glucosaminyl-(1-&gt;2)-O-alpha-D-mannosylprotein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the glycosyltransferase 13 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013911709.1">
        <property type="nucleotide sequence ID" value="XM_014056234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X865"/>
    <dbReference type="GeneID" id="106540928"/>
    <dbReference type="KEGG" id="tsr:106540928"/>
    <dbReference type="CTD" id="55624"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047223">
        <property type="term" value="F:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02514">
        <property type="entry name" value="GT13_GLCNAC-TI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13937">
        <property type="entry name" value="PANDER_GnT-1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000038">
        <property type="entry name" value="protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004139">
        <property type="entry name" value="Glyco_trans_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039477">
        <property type="entry name" value="ILEI/PANDER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052463">
        <property type="entry name" value="O-linked_mannose_GnT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039474">
        <property type="entry name" value="POMGNT1_PANDER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46396">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46396:SF1">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03071">
        <property type="entry name" value="GNT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15711">
        <property type="entry name" value="ILEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52031">
        <property type="entry name" value="GG_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 18" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 19" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ILEI/PANDER" evidence="20">
        <location>
            <begin position="122"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF15711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013911709.1"/>
        </source>
    </evidence>
    <sequence length="634" mass="72849" checksum="CDF8F551A63C0F72" modified="2020-10-07" version="1">MDEWKPNPQPKPWKKRSWYLAWKYKLTNQRALRRLCQMGAVLFLLVTVIVNIKLILDTRRAANEEDDSAHDYDESLQKLEIPRQPINSRKVLDIEAYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVLFLNMVAKGRILIFTIKDEGSFHLKDTAKSLLKSLGSQIAASLSWRDMWTFVGKKGGEVYGEKHAKSALLSSWGEPVLLKTEVQLTSVEAIHSGSWKTTYPQVANVEKHCLKCSSLKDNKVFDVPVAVIAGNRPNYLYRMLRSLLSAQGVNPQMITVFIDGYYEEPMDVVELFGLKGIQHTPISIKNARVSQHYKASLTATFNLHPDAKFAVVLEEDLDLSIDFFSFLSQSIYLLEEDDSLYCISAWNDQGYEHTAEDPSLLYRVETMPGLGWVLRKTMYKDELEPKWPTPEKLWDWDMWMRMPEQRKGRECLIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFNTVPNVQLKNVESLKKDAYEIELHRLLSEAEVLDHSKNPCEDSFVPDTEGKTYVMYIKMEQEADFNTWTQLAKCLHIWDLDVRGNHKGLWRLFRKKNHFLVVGVPASPYSFKKPPSVIPIYIEPPAKDEAAGAEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X880</accession>
    <name>A0A6I9X880_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">N-acetyltransferase ESCO1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ESCO1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. ECO subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911724.1">
        <property type="nucleotide sequence ID" value="XM_014056249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X880"/>
    <dbReference type="GeneID" id="106540936"/>
    <dbReference type="KEGG" id="tsr:106540936"/>
    <dbReference type="CTD" id="114799"/>
    <dbReference type="OrthoDB" id="428854at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028005">
        <property type="entry name" value="AcTrfase_ESCO_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE ESCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13878">
        <property type="entry name" value="zf-C2H2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO zinc-finger" evidence="12">
        <location>
            <begin position="761"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="13">
        <location>
            <begin position="919"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="152"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="278"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="213"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013911724.1"/>
        </source>
    </evidence>
    <sequence length="1001" mass="113218" checksum="04C5A9FA2A5E0F04" modified="2020-10-07" version="1">MAAQKRKSVLVESSAKRRKLNKSNKSVSTSKERKVQDAKCISNKKAVPKKEVPQLSATTNRSAVNKFINRAALPYSQNKQSQRNSKQTLNELKKKACKMVCMKRTSLTSLGKLRPAQTSLKSSKLKHANSLNLSKSSSKICSRGKRKCNIVKNKTSYENKKPNKSPVDSKTRSTEAQCKRHAARCGKTSHKNLHSKSRPIKENTRRITKSGKCISSSNLEVQNSKTPSLKKTASQNSEARWTKTSSEKPCDIDHRNIRKKSVGTDSVKAVRIRQEQLSVKQTKKVPEKDTVQDLSKSQHTVSHGHQTRRSPRFQQSSDVSSISLRSRKVKETRASVTKQCNQMKKLMPQLKPEKLKHVHQKPGKSAEDNDHQIERRKISDNKKRGCFSPKKHEADSEISDSIISQDKPRELRNNLESNKESEKVANTISCPINSACSIISKAKSKHKNGKIVVKADRVLPVQPVCQREDVVKSKKISILELCEEIAGEIESDTVEVKRISPNPDYGKEEEKNPVLELPQDIVLNDGKTNQNSQCKRFFPSKKGMSVKCTVNGRHSTVNKNSKWTKIKLTRINHVSHSISSTPKLDVLKCNDPEEQQARTVEIHLPEVQRKLSQHNDSTICEQKLNKPDFEGIQIKDRILHAKQSTVQKIENGLSEAKHCPGPTPDKSFNLVFATHPENTSVKIISEQESVVKQMKRETTEIKPQDPVGKQLVQTPANKTSEINESRASMPKIPLTTKCDGFLTSEEHIQKLKEAAKDGDKQLIIDAGQKRFGAISCNICGMLYTASNPEDETQHLFFHNQFISAVKYVGWKKERILSEYPDGKIIMVLPDDPKYALRKVDEIREMVDNDLGFQQAPLTCSRTKTLLFISNDKKVIGCLIAEYIQWGYRVIEEKIPDISSENDKLTFERQKAWCCSTTPEPAICGISRIWVFSMMRRKKIASRMLECLRNNFIYGSYLSKEEIAFSDPTPDGKLFATRYFGTSQFLVYNFLSGQQPQSGNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X888</accession>
    <name>A0A6I9X888_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">XRCC6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ku70 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013907538.1">
        <property type="nucleotide sequence ID" value="XM_014052063.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907539.1">
        <property type="nucleotide sequence ID" value="XM_014052064.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907540.1">
        <property type="nucleotide sequence ID" value="XM_014052065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X888"/>
    <dbReference type="GeneID" id="106537808"/>
    <dbReference type="KEGG" id="tsr:106537808"/>
    <dbReference type="CTD" id="2547"/>
    <dbReference type="OrthoDB" id="3249161at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00788">
        <property type="entry name" value="KU70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01458">
        <property type="entry name" value="vWA_ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000007">
        <property type="entry name" value="X-ray repair cross complementing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000001">
        <property type="entry name" value="X-ray repair cross complementing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.970.10:FF:000001">
        <property type="entry name" value="X-ray repair cross-complementing protein 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1600.10:FF:000001">
        <property type="entry name" value="X-ray repair cross-complementing protein 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000080">
        <property type="entry name" value="X-ray repair-complementing defective repair in Chinese hamster cells 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.970.10">
        <property type="entry name" value="Ku70, bridge and pillars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027388">
        <property type="entry name" value="Ku70_bridge/pillars_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047087">
        <property type="entry name" value="KU70_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00578">
        <property type="entry name" value="ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003033">
        <property type="entry name" value="Ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="16">
        <location>
            <begin position="574"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="535"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA; for 5'-deoxyribose-5-phosphate lyase activity" evidence="14">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR003033-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013907538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013907539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013907540.1"/>
        </source>
    </evidence>
    <sequence length="611" mass="70392" checksum="C865DBFC5C69B187" modified="2020-10-07" version="1">MSNWVSYYKNEGEEEEEEEQEGAENDGVYKYGGRDTLLFLVDGSRRMFESFDGSSLTPFDMTIQCIQNVYRNKIISHERDLVGVVFYGTEQHNNTMNREHIYVLQNLSNPGAKRVLELASYQGEQGKALFQKSFGHSDDYSLGEALWVCSNLFSVVQFKMSHKRIMLFTNEDDPHGQDSTKTKFARTKAADLRETGIYLDLMNLKKPGGFDISLFYRDIINTADDEDLGIQFEESCKLEDLMKKVRAKEIRKRALSRLNFYLGKDVTLTVGVFNLVQKAVKQPPVKLYRETNEPLKTKTRTFSRETGSLLLPSDTKRAQIYGNRKIVLEKEETEEIKRFDTPGLYLIGFKPLVMLKPYHYIRPAQFIYPDEFLMNGSTTLFNALLTKCLEKGVMAICRYTPRQNNPPRFIALVPQEEELDEQNVQIVPAGFHLIFLPYADDKRNIDFTEKIPANQQQVNKMKEIIQKLRFKYRSESFENPVLQQHYMNLEALALDLMEPEKAEDLTVPKVELMDVRLGNLAEEFKQLVYPPGYDPDGKTIKRKQAGSGTEQPEKKARMELSEKEVRNHVDKGTLGKLTVPILKDICRIYKLTGGGKKQELLDAITAHFNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9Q9</accession>
    <name>A0A6I9X9Q9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S15a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">RPS15A</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910491.1">
        <property type="nucleotide sequence ID" value="XM_014055016.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910492.1">
        <property type="nucleotide sequence ID" value="XM_014055017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9Q9"/>
    <dbReference type="SMR" id="A0A6I9X9Q9"/>
    <dbReference type="GeneID" id="106540039"/>
    <dbReference type="KEGG" id="tsr:106540039"/>
    <dbReference type="CTD" id="6210"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013910491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013910492.1"/>
        </source>
    </evidence>
    <sequence length="130" mass="14840" checksum="D4AC1E8864E3A184" modified="2020-10-07" version="1">MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XA25</accession>
    <name>A0A6I9XA25_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14 15">CUGBP Elav-like family member 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14 15 16" type="primary">CELF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CELF/BRUNOL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910627.1">
        <property type="nucleotide sequence ID" value="XM_014055152.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910628.1">
        <property type="nucleotide sequence ID" value="XM_014055153.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910629.1">
        <property type="nucleotide sequence ID" value="XM_014055154.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910630.1">
        <property type="nucleotide sequence ID" value="XM_014055155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XA25"/>
    <dbReference type="GeneID" id="106540127"/>
    <dbReference type="KEGG" id="tsr:106540127"/>
    <dbReference type="CTD" id="10658"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12631">
        <property type="entry name" value="RRM1_CELF1_2_Bruno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12634">
        <property type="entry name" value="RRM2_CELF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12638">
        <property type="entry name" value="RRM3_CELF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000013">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000015">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000016">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034196">
        <property type="entry name" value="CELF1/2_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034198">
        <property type="entry name" value="CELF1/2_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034199">
        <property type="entry name" value="CELF1/2_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="43"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="135"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="431"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="306"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013910628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013910629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910630.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="55286" checksum="73B178CB42962D80" modified="2020-10-07" version="1">MAAFKLEFLPEMMVDHCSLNASPISKKMNGTLDHPDQPDLDAIKMFVGQVPRSWSEKDLRDLFEQYGAVYEINVLRDRSLNPPQSKGCCFVTFYTRKAALEAQNALHNMKILPGMHHPIQMKPADSEKNNAVEDRKLFIGMISKKCNENDIRMMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRGMAQTAIKAMHQAQTMEGCSSPIVVKFADTQKDKEQKRMAQQLQHQMHQISAASVWGNLAGLNTLGPQYLALYLQLLQQTAAASSGNLNTLGNLHPMGGLNAMQLQNLAALAAAASAAQNTPSGTNALNSSNSPLSVLTSSAGSSPSSSSSSSVNPMASLGALQTLAGATAGLNVGSLAGMAALNGGLGSGGLSNGTGSTMEALTQAYSGIQQYAAATLPTLYNQSLLTQQSIGAAGSQKEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNGFQIGMKRLKVQLKRSKNDSKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC99</accession>
    <name>A0A6I9XC99_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rac GTPase-activating protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911477.1">
        <property type="nucleotide sequence ID" value="XM_014056002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC99"/>
    <dbReference type="GeneID" id="106540758"/>
    <dbReference type="KEGG" id="tsr:106540758"/>
    <dbReference type="OrthoDB" id="2218807at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097149">
        <property type="term" value="C:centralspindlin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1930">
        <property type="entry name" value="Uncharacterised protein PF16691, DUF5062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038316">
        <property type="entry name" value="DUF5062_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46199">
        <property type="entry name" value="RAC GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46199:SF4">
        <property type="entry name" value="RAC GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911477.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="15897" checksum="08D0A18BE98B82C1" modified="2020-10-07" version="1">MQHLDQLLELGNFNSNIEEEYLQIAKCLEEMRQRCCRLDHDLQKARQQLRQSESEFSVLEVKLKHARNQVEVEMKKRRWAEAELEKQECKLQLIYDYLMADPQIAPLTEDKHADLAIVERLCFSRSNLLPRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDK3</accession>
    <name>A0A6I9XDK3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyhomeotic-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PHC3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912286.1">
        <property type="nucleotide sequence ID" value="XM_014056811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDK3"/>
    <dbReference type="GeneID" id="106541383"/>
    <dbReference type="KEGG" id="tsr:106541383"/>
    <dbReference type="CTD" id="80012"/>
    <dbReference type="OrthoDB" id="2390104at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912286.1"/>
        </source>
    </evidence>
    <sequence length="779" mass="82896" checksum="2B2363E44D425474" modified="2020-10-07" version="1">MENESSTTTSSTGTTTIATTSSPSRTQPPQISVYSGSDRHAVQVIQQALHRPPSSAAQYLQQMYAAQQQHLMLQTAALQQQHLSSTPFQSLANVPQANLSGGRQCASPNGSVSQQSSMSETSINLSASPTPAQLISRSQTSNTSSSSLTQQTMLLGSTSPNLTASQAQMYLRAQMLIFTPATTVAAVQSDIPVVSSSSSFPCQSAATQVQNLTLRSQKLGVLSTSQNGTPKNASQPQTLAVCPSKSVTGAKASQSDLESKRKGDSPNAEPRSPAVTRTSSIHQLMAPASYSPMQPHSLLKHQHLPLHSPPSKLSHHQLIFQQQQVQPITLQLPPSHEATPSQHCAPLQGHPLTPDPAITPSQHCTPVHVHPPPLSPTPSPSPQQSVVVSPLPPTSPSQSPSIIIHPQTLIQSHPLTSSALQSEHNSVQADESSTESTSQSAAHPPQLSPSPLVHGGPADRPSMLSPSQQMSSPSSHQPYPTLHPAPVPLATSPQLSSTPAHPQQMSLPPVPSLQVQPELLSQDQVLVQNTLVSEEELPAAEALVQLPLQTLPPPQTVAVNLQVQQAVPIETPVLYQEEDVCEDEMPEDSDGVRMVRTPTPPTLSPPAITLRSREELSYEDAFAEQEGHSSMASSVSASVIKSPSDPSHASIPLPPLLLPAIATRTHSASVSNRNPSIEIKLPQAIVKPQILTHVIEGFVIQEGLEPFPVSCSSLLTDQPAKKRLLLEEQVVNVMCVEPELQNNAKHADNSSDTEMEDIITEGALNIIQNDLPTFGIPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFK1</accession>
    <name>A0A6I9XFK1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Popeye domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">POPDC3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the popeye family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914444.1">
        <property type="nucleotide sequence ID" value="XM_014058969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFK1"/>
    <dbReference type="GeneID" id="106543064"/>
    <dbReference type="KEGG" id="tsr:106543064"/>
    <dbReference type="CTD" id="64208"/>
    <dbReference type="OrthoDB" id="425611at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051146">
        <property type="term" value="P:striated muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006916">
        <property type="entry name" value="POPDC1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055272">
        <property type="entry name" value="POPDC1-3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101">
        <property type="entry name" value="POPEYE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101:SF18">
        <property type="entry name" value="POPEYE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04831">
        <property type="entry name" value="POPDC1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="POPDC1-3" evidence="8">
        <location>
            <begin position="26"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914444.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="33602" checksum="1135D8A78374A875" modified="2020-10-07" version="1">MEDNSSFWDSLVFSHPICMNWKSETEGSIYHLSSILFVVGFMGGSGFFGLLYIFFLLGLGFLCSSVWSWLDVCAADIFSWNFILFVICFVQFIYVTYQIRSVAFDKEFQELYNALFQPLGISLPVFRNIVLCCGGEVITLEKEHCYAMQGKTPIDKLSLLLSGRVSVTVDGEFLHYIFPLQFLDSPEWDSLRPTEDGIFQVTLTAETDCHYVAWRRKKLYLLFAKHRFISRLFSILIGNDIADKLYALNDRVSQGKGFRYDIRLPNYYHVSAPNTPQKKSAHHFQNNSRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIT3</accession>
    <name>A0A6I9XIT3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome activator complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Activator of multicatalytic protease subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Proteasome activator 28 subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PSME3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910833.1">
        <property type="nucleotide sequence ID" value="XM_014055358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIT3"/>
    <dbReference type="GeneID" id="106540292"/>
    <dbReference type="KEGG" id="tsr:106540292"/>
    <dbReference type="CTD" id="10197"/>
    <dbReference type="OrthoDB" id="6591885at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008537">
        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.180:FF:000001">
        <property type="entry name" value="Proteasome activator complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.120:FF:000001">
        <property type="entry name" value="Proteasome activator complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.120">
        <property type="entry name" value="Proteasome activator pa28, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036996">
        <property type="entry name" value="PA28_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009077">
        <property type="entry name" value="Proteasome_activ_PA28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003185">
        <property type="entry name" value="Proteasome_activ_PA28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660:SF4">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660">
        <property type="entry name" value="PROTEASOME REGULATOR PA28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02251">
        <property type="entry name" value="PA28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 N-terminal" evidence="7">
        <location>
            <begin position="46"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="8">
        <location>
            <begin position="145"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013910833.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="33291" checksum="FC4AFF91EA39CB5E" modified="2020-10-07" version="1">MLSLPAVTKPVVFSGGRARGIKGNRSGSEYWLPRPTMASLLKVDPEVKIKVDSFRERITSEAEDLVANFFPKKLLELDGFLKDPILNIHDLTQIHSDMNLPVPDPILLTNSHDGLDGSNMKKRKLEDCEETFQGTKVFVMPNGMLKSNQQLVDIIEKVKPEIRLLIEKCNTVKMWVQLLIPRIEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITRAKLVSKIAKYPHVEDYRRTVTEMDEKEYISLRLIISELRNQYVTLHDMILKNIEKIKRPRSSNAETLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK61</accession>
    <name>A0A6I9XK61_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">THAP domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">THAP4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>peroxynitrite = nitrate</text>
            <dbReference type="Rhea" id="RHEA:63116"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:25941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:63117"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="RefSeq" id="XP_013916189.1">
        <property type="nucleotide sequence ID" value="XM_014060714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK61"/>
    <dbReference type="GeneID" id="106544445"/>
    <dbReference type="KEGG" id="tsr:106544445"/>
    <dbReference type="CTD" id="51078"/>
    <dbReference type="OrthoDB" id="58529at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd07828">
        <property type="entry name" value="lipocalin_heme-bd-THAP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045165">
        <property type="entry name" value="Nitrobindin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014878">
        <property type="entry name" value="THAP4-like_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15854:SF4">
        <property type="entry name" value="PEROXYNITRITE ISOMERASE THAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15854">
        <property type="entry name" value="THAP4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08768">
        <property type="entry name" value="THAP4_heme-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THAP4-like heme-binding" evidence="2">
        <location>
            <begin position="58"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00036993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916189.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="24005" checksum="1A86523E048D1CA6" modified="2020-10-07" version="1">MVICCAALNCSNRQGKGPRGRVAVSFHRLPIATFKKLVQFDTLRLFIYLETAQLNPVIEPLSWMLGTWLSDPPGDGTFPSMKPFQYLEEVHISHAGQPMLNFSFNAFHPDTKKPMHRECGFIRIQPDTNKVAFISAQNTGLVEVEEGEVNGQELSIASHSVARISFAKKPHVEQITRKFRLNSEGKLEQTVSMSTTTQPMTQHLHITYKKVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKY7</accession>
    <name>A0A6I9XKY7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adhesion G-protein coupled receptor D1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106544664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916489.1">
        <property type="nucleotide sequence ID" value="XM_014061014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKY7"/>
    <dbReference type="GeneID" id="106544664"/>
    <dbReference type="KEGG" id="tsr:106544664"/>
    <dbReference type="OrthoDB" id="347083at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026794025" evidence="1">
        <location>
            <begin position="25"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916489.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="15418" checksum="19DCC085B711B9D8" modified="2020-10-07" version="1">MATWLVQLCYLLLWILFQVYRTHTWLPKHPGFRVLAQASHYWPLETVEGIHELKDTDGVLRSHSFTVLHYHNSTFVYTNDSAYSNFSATVDMVEGIVNKGVYIPSIKGETFLLFGNSQNSCIINQEFCEPEDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLT6</accession>
    <name>A0A6I9XLT6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Heme transporter HRG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SLC48A1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>heme b(in) = heme b(out)</text>
            <dbReference type="Rhea" id="RHEA:75443"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911798.1">
        <property type="nucleotide sequence ID" value="XM_014056323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLT6"/>
    <dbReference type="GeneID" id="106541005"/>
    <dbReference type="KEGG" id="tsr:106541005"/>
    <dbReference type="CTD" id="55652"/>
    <dbReference type="OrthoDB" id="5954402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026218">
        <property type="entry name" value="HRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31525">
        <property type="entry name" value="HEME TRANSPORTER HRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31525:SF1">
        <property type="entry name" value="HEME TRANSPORTER HRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16954">
        <property type="entry name" value="HRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02095">
        <property type="entry name" value="TRNSPORTRHRG"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911798.1"/>
        </source>
    </evidence>
    <sequence length="89" mass="10612" checksum="0309651A7D75F6D2" modified="2020-10-07" version="1">MYMQDYWRTWLKGLRFFMCVGIFFGILSLVGFCTFLALAIMKKQSFTDPQSYYLSCIWSILSLKWAFLLGLYSHRYKKEFADISILSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM86</accession>
    <name>A0A6I9XM86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ras-related protein Rab-15</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22 23 24" type="primary">RAB15</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22 23 24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB15 may act in concert with RAB3A in regulating aspects of synaptic vesicle membrane flow within the nerve terminal.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">The GTP bound form of RAB15 interacts with REP15. Interacts (GTP-bound form) with MICAL1, MICAL3, MICALCL, EHBP1 and EHBP1L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013915017.1">
        <property type="nucleotide sequence ID" value="XM_014059542.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915023.1">
        <property type="nucleotide sequence ID" value="XM_014059548.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915032.1">
        <property type="nucleotide sequence ID" value="XM_014059557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM86"/>
    <dbReference type="GeneID" id="106543503"/>
    <dbReference type="KEGG" id="tsr:106543503"/>
    <dbReference type="CTD" id="376267"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04117">
        <property type="entry name" value="Rab15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000990">
        <property type="entry name" value="ras-related protein Rab-15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041826">
        <property type="entry name" value="Rab15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013915017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013915023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013915032.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="24374" checksum="F40C087EB4D3A10D" modified="2020-10-07" version="1">MAKQYDVLFRLLLIGDSGVGKTCLLCRFTDNEFHPSHISTIGVDFKMKTIEVDGIKVRIQIWDTAGQERYQTITKQYYRRAQGIFLVYDISSERSYQHIMKWASDVDEYAPDGVQKILIGNKADEEQKRQVGREQGLQLAKEYGMDFYETSACTNLNIKESFTRLTELVLQAHKKELDGLRTYAADELNVVELEEEASKPEGSEHSPKTCWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNI0</accession>
    <name>A0A6I9XNI0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear prelamin A recognition factor-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NARFL</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915512.1">
        <property type="nucleotide sequence ID" value="XM_014060037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNI0"/>
    <dbReference type="GeneID" id="106543930"/>
    <dbReference type="KEGG" id="tsr:106543930"/>
    <dbReference type="CTD" id="64428"/>
    <dbReference type="OrthoDB" id="10253113at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000042">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003149">
        <property type="entry name" value="Fe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02256">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00902">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase small subunit" evidence="7">
        <location>
            <begin position="410"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013915512.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="53193" checksum="CD747C88F3D3E915" modified="2020-10-07" version="1">MAVASPFSGVLQLTDLDDFIGPSQECIKPIKVDKPGKAAARIRIEDDGTYFQVGQDGGPQKLEKAKITLNDCLACSGCITSAESVLITQQSHEELYKVLHANKASNSPKKLVVVSVSPQSRASLAARFGMTPLDTAKKLTSFFKNLGVHFVLDTTFTRNFSLLESQREFVQRFQRRAEDKKSLPMLAAACPGWICYAEKTHGNFIIPYISTTRSPQQIMGALIKDHFAKQQCLTPDQIYHATVMPCYDKKLEASRPDFFIQKYQTREVDCVITTGEVLKLLDEEGVSLLEVNPMPLDSMFGNCLEDQLLGHPGGGSGGYLEHIFKYAAKELFGIPVKELQYKTLKNKDFQEVTLEKDGQTLLHFALAYGFRNIQNFVQKLKRGKLPYHYVEVMACPSGCLNGGGQIRAEGESSKDLLHHVERLYEMAQPEDPEMNKAISDLYDQWLGGPTSPQAQDHLHTQYHAVEKGSTSFNIKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRQ0</accession>
    <name>A0A6I9XRQ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Hepatocyte nuclear factor 4-alpha isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HNF4A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916742.1">
        <property type="nucleotide sequence ID" value="XM_014061267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRQ0"/>
    <dbReference type="GeneID" id="106544866"/>
    <dbReference type="KEGG" id="tsr:106544866"/>
    <dbReference type="CTD" id="3172"/>
    <dbReference type="OrthoDB" id="5771769at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06960">
        <property type="entry name" value="NR_DBD_HNF4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06931">
        <property type="entry name" value="NR_LBD_HNF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000007">
        <property type="entry name" value="Hepatocyte nuclear factor 4 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000012">
        <property type="entry name" value="Hepatocyte nuclear factor 4, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049636">
        <property type="entry name" value="HNF4-like_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049635">
        <property type="entry name" value="HNF4_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="59"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="149"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013916742.1"/>
        </source>
    </evidence>
    <sequence length="467" mass="52084" checksum="5CDE65734D99BD94" modified="2020-10-07" version="1">MIMRLSKALIDMEMADYSAALDPAYTTLEFENMQVLAMGSDTSPSEVTNLNASNNIGASVLCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFNRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINVLLQAEVLSQQISSPMSGISGDIRGKKIANIADVCESMKQQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGAAKRSMVFKDILLLGNDHIIPRNCPELLEISRVAVRILDELVLTFQDLQIDDNEYACLKAIIFFDPDAKGLSDPIKIKRMRYQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFVKLFGMAKIDNLLQEMLLGGSSSDTPHAHHHPLHPHLIQENLGTNVIVANTMPPQIHNGQLSTPETPQPSPPAGSGAEQYKLLPGAIATVAKQPSSIPQPTITKQEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRY8</accession>
    <name>A0A6I9XRY8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MKRN2 opposite strand protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MKRN2OS</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913463.1">
        <property type="nucleotide sequence ID" value="XM_014057988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRY8"/>
    <dbReference type="GeneID" id="106542302"/>
    <dbReference type="KEGG" id="tsr:106542302"/>
    <dbReference type="CTD" id="100129480"/>
    <dbReference type="OrthoDB" id="10065749at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032016">
        <property type="entry name" value="MKRN2OS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053921">
        <property type="entry name" value="MKRN2OS-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053922">
        <property type="entry name" value="MKRN2OS-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33963">
        <property type="entry name" value="MKRN2 OPPOSITE STRAND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33963:SF2">
        <property type="entry name" value="MKRN2 OPPOSITE STRAND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16044">
        <property type="entry name" value="DUF4796_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22795">
        <property type="entry name" value="DUF4796_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MKRN2 opposite strand protein-like N-terminal" evidence="2">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="MKRN2 opposite strand protein-like C-terminal" evidence="1">
        <location>
            <begin position="60"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913463.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="26816" checksum="FEE8C08235549944" modified="2020-10-07" version="1">MQVGKQVHLPKEGLIMQPLDTERALIKVTHCDKDIFGCLVPESCPLCGQRLIYGSLEEAPVSIPSPFVNGHQERCAFLLKPTIGTFLRDYDGDSDLHVGITNTNGLVYNYNETGITIAEYGWEQCVSIPLVQPDMYGLLNQWDTYLGQFSATDIWLPHRYEECFHNCYTYALTFINCVLSAQKQQQMNKNEFAEKFVIPRTRKASKYITIYKEICKNGFYVVDHPAQKGSTSTNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVC2</accession>
    <name>A0A6I9XVC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Caspase-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CASP9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920084.1">
        <property type="nucleotide sequence ID" value="XM_014064609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVC2"/>
    <dbReference type="GeneID" id="106547434"/>
    <dbReference type="KEGG" id="tsr:106547434"/>
    <dbReference type="CTD" id="842"/>
    <dbReference type="OrthoDB" id="6044770at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08326">
        <property type="entry name" value="CARD_CASP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000012">
        <property type="entry name" value="Caspase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042147">
        <property type="entry name" value="CARD_CASP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901:SF8">
        <property type="entry name" value="CASPASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="CARD" evidence="11">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="10">
        <location>
            <begin position="442"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="9">
        <location>
            <begin position="614"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="175"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="576"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013920084.1"/>
        </source>
    </evidence>
    <sequence length="700" mass="76350" checksum="D8576A96DD27C7D1" modified="2020-10-07" version="1">MEEADRRRLLRSRVQLVRKLQLEPLWAPLLRRGIFTANMIEEIQSAGPRPDQARKLVVDLATRGRRALAEFVLCLRESGQEDLAELLSDGRSPEGAPDQPPGHPRRPREQALHKLPEVLPDDRVVEEGDLPVDQAQDGRGAVRSKVLGQAPFVARMDDTDPHTWQGEDKALNREATQGPGRALGLPCSGGAEASGSGLTSDDLRRPPPNPPDQPFRMPRGSPSPKSNARPLTLSFTCCPSPPSAPRLTQRLQLLHHLLPDRLDVDAAEAPVGGDLDEVRLPEALGDDLVDVVGEVEQVLLVEGVLVALRVEGEVEEGLVKVEALVEGMCMAVRRLLLLLVREALVLAHVAVTVKIQPLLLVGQDLRGKSRSLVPSFPPDRPSRSPNPLQLSLSYRSAPTVCGLQPFPLSSLSPSLPLSGPLGPLEETPQRDSEMVYSLNSEPCGYCLIINNVHFDKRTGLKLRQGSDVDRQRLEKRFRALHFEVLAKEDLTAQGIAQELQSLAGRNHSGLDCCLVVILSHGCESYHIQIPGAIFGTDGQRIPVQKVVSYFNGSHCPSLRGKPKLFFIQACGGEEKDPGFEVETDATPGQRGGEAVESDATPTPGPGGDSDELDATASLPTDSDILVCYPTFPGHVSWRDTQSGSWFIEALDQVLAEKAHLEDLPSMLTKVGNIVADKVAGPYKQMPGYFNFLRKKFFFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVQ3</accession>
    <name>A0A6I9XVQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-protein ligase E3C</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HECT-type ubiquitin transferase E3C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RTA-associated ubiquitin ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">UBE3C</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with 26S proteasomes. Interacts (via the HECT domain) with UBE2D1 and, less efficiently, with UBE2L3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UBE3C family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013914723.1">
        <property type="nucleotide sequence ID" value="XM_014059248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVQ3"/>
    <dbReference type="GeneID" id="106543269"/>
    <dbReference type="KEGG" id="tsr:106543269"/>
    <dbReference type="CTD" id="9690"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000002">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000011">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000014">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF2">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="17" id="KW-0436">Ligase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="743"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013914723.1"/>
        </source>
    </evidence>
    <sequence length="1082" mass="124094" checksum="9C0DB188AE315524" modified="2020-10-07" version="1">MFSFEGDFKTRPKVSLGGASKKEEKASLLHRTQEERRKREEERRRLKNAVIIQSFVRGYRDRKHQYAIQRSEFDRYANLAQSGGSCSSAIGANLTLLVRQLLFFYRYNEDSKRLIWICQNLIKQNSQFVKQLEGPDRFTCLYQIKRLLGLCCRLLQSCNDDSLNVALPMRMLEVFSSENTYLPVLQDANNVASIIEQILHYVIQNGYYRSLYLLINNKLPSSIEYSDVSRVPIAKIFLENVLKPLHFNYSSCPEVARQQVFAAFTEEFLAAPFTDQIFHFIIPALVDGETCFPYELFLNALLFTESGHLRERERAPWLFYFVLIAGETYLGSLSEEGLLVYLRVLQSFLSQLPVSMSGTDCQDSASDSEDEEISKMSSMPGDGRISVQYITEECLSKLDTKQQTNTLLNMVWRDSASEEVFTLMASICHTLMVQHRMMVPKVRLLYSLAFNARFLRHLWYLISEMKTRMITGSMVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVTLSRCLRDACLGIIKLAYPETKPEVREEYIAAFRSIGVTTNTEMQQCIQTEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPDHWLSLQENIRADKVTQLYVPSARHVWRVRRMGRIGPLQSTLDVGLDPAPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGEGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGGSYARHYYFLGRMLGKALYENMLVELPFASFFLSKLLGTSADVDIHHLASLDPEMYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKLGGKDIPVTSANRIAYIHLVADYRLNKQIRQHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAQVPISLEDLKTFTNYSGGYTADHPVIKIFWRVVESFTDDEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFCDEHLMRSKLLYAIECAAGFELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW60</accession>
    <name>A0A6I9XW60_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GNG10</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Interacts with beta-1 and beta-2, but not with beta-3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">G proteins are composed of 3 units, alpha, beta and gamma.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Lipid-anchor</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914888.1">
        <property type="nucleotide sequence ID" value="XM_014059413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW60"/>
    <dbReference type="GeneID" id="106543404"/>
    <dbReference type="KEGG" id="tsr:106543404"/>
    <dbReference type="CTD" id="2790"/>
    <dbReference type="OrthoDB" id="6264244at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="13">
        <location>
            <begin position="29"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013914888.1"/>
        </source>
    </evidence>
    <sequence length="74" mass="8063" checksum="4C374A2DE54FF669" modified="2020-10-07" version="1">MDSNIYKCLQPGLSINIERSNVACHFLVLPLIQVSQAAAELQQYCMQNACKDALLVGVPAGSNPFREPRSCALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWS4</accession>
    <name>A0A6I9XWS4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ES1 protein homolog, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106538875</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908980.1">
        <property type="nucleotide sequence ID" value="XM_014053505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWS4"/>
    <dbReference type="GeneID" id="106538875"/>
    <dbReference type="KEGG" id="tsr:106538875"/>
    <dbReference type="CTD" id="106538875"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03133">
        <property type="entry name" value="GATase1_ES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000035">
        <property type="entry name" value="ES1 protein homolog, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008747">
        <property type="entry name" value="PRK11780.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10224">
        <property type="entry name" value="ES1 PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10224:SF9">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE-LIKE CLASS 1 DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026834904" evidence="4">
        <location>
            <begin position="29"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="DJ-1/PfpI" evidence="5">
        <location>
            <begin position="53"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013908980.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="28165" checksum="F8E1FE416A2322E7" modified="2020-10-07" version="1">MWAAKFANSRSPLLSSVLLLSKGAPASSSPFHSSAHLHKAKVAVVLSGCGVYDGTEIHEASAIMVHLSRGGANVDMYAPNISQMHVVDHSKGQPADRESRNVLTESARIARGKINDLARLTARDYDAVVFPGGFGAAKNLSTFSVDGKDCKVNKDVERVLKDFHKSGKPIGLCCISPVLAAKVLPGTEVTVGQEQEQGGKWPHASTAGAIKAMGGKHCAKDVTEAHVDNTNKVVTTPAFMCETEFHHIFDGIGAMIKNLLRLTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX46</accession>
    <name>A0A6I9XX46_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DAZ-associated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Deleted in azoospermia-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DAZAP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918737.1">
        <property type="nucleotide sequence ID" value="XM_014063262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XX46"/>
    <dbReference type="GeneID" id="106546397"/>
    <dbReference type="KEGG" id="tsr:106546397"/>
    <dbReference type="CTD" id="26528"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12574">
        <property type="entry name" value="RRM1_DAZAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12327">
        <property type="entry name" value="RRM2_DAZAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000129">
        <property type="entry name" value="DAZ-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000093">
        <property type="entry name" value="Putative DAZ-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034134">
        <property type="entry name" value="DAZAP1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034131">
        <property type="entry name" value="DAZAP1_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF18">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="112"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="76"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="184"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="285"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013918737.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="43527" checksum="7830686E03CBFB20" modified="2020-10-07" version="1">MNSVGGADEIGKLFVGGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTVLASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWKGSRSDSKSHKIFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQTPGPTGTSQWGSRIMPSAANGWAGQPPPTWQQGYGPQGMWVPAGQALGGYGPPPPGRGAPPPPPPFTSYIVSTPPGGFPPPQGFAQGYGTPPPFSFGYGAPPPPPDQFAPPGVPPPPATPGATPLAFPPPPPPSQPTQDMNKPPAAQPDFPYSQFGYGQDLSGFGQGFADPSQQPPSYGGPSVPPSSGPPAGGSGFGRGQNHNVQGFHPYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXX0</accession>
    <name>A0A6I9XXX0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15 16">Probable ribonuclease ZC3H12C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17" type="primary">ZC3H12C</name>
    </gene>
    <organism evidence="13 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915440.1">
        <property type="nucleotide sequence ID" value="XM_014059965.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915441.1">
        <property type="nucleotide sequence ID" value="XM_014059966.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915442.1">
        <property type="nucleotide sequence ID" value="XM_014059967.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915443.1">
        <property type="nucleotide sequence ID" value="XM_014059968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXX0"/>
    <dbReference type="GeneID" id="106543866"/>
    <dbReference type="KEGG" id="tsr:106543866"/>
    <dbReference type="CTD" id="85463"/>
    <dbReference type="OrthoDB" id="392925at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18729">
        <property type="entry name" value="PIN_Zc3h12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040546">
        <property type="entry name" value="Rege-1_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040757">
        <property type="entry name" value="Regnase_1/ZC3H12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF36">
        <property type="entry name" value="RIBONUCLEASE ZC3H12C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18561">
        <property type="entry name" value="Regnase_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18039">
        <property type="entry name" value="UBA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="423"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="496"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013915440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013915442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013915443.1"/>
        </source>
    </evidence>
    <sequence length="854" mass="96519" checksum="5586CEC37FBC5B18" modified="2020-10-07" version="1">MGLKDHLKHDLGHLYEDTSVTQISAIAPWSMIEKPTMDKINSRKEDPKKELSEETGTFSRDSEKNIHSVNELGKATKIRTEPCSLSKDHRQTYPSLEPHILKRNEILQDIKLEEVQIGSKEMKKPPDMVKEYQTKLDFALKLGYSEEQVQLVLNKLGTDALINDILGELVKLGNKTEADQSVSSINTSVMREASSVDSQRSDSPLQEDVLVDGDNLRPIVIDGSNVAMSHGNKEVFSCRGIKLAVDWFLERGHKDVTVFVPAWRKEQSRPDALITDQEILRKLEKEKILVFTPSRRVQGRRVVCYDDRFIVKLAFESDGIIVSNDNYRDLANEKPEWKKFIDERLLMYSFVNDKFMPPDDPLGRHGPSLENFLRKKPVVPEHKKQPCPYGKKCTYGHKCKYYHPERGNQPQRSVADELRAMSRNTAAKAASEGGLVKSNSVPCSTKTDNTTDLKRTTPKRQSDPSIRTQVYQDLEEKLPTKNKLGTRSVPSLVSIPSSAAAKPQSTAPLTNGLPSGVHFPPQDQRPQGPYSPVIMTTKNHGTPMPYEQYPKCDSPVDVGYYSMLSAYSNLSISGPRSPERRFSLDTDYRISSVASDCSSEGSMSCGSSDSYMGYNDRSYVSSPDPQLEENLKCQHMHPHSHLNSQSFLQSYHDTLTRVQSYNHEEPKHHPKPPAPYMAVHFQHPNMGSRSTCPLDFPASQSSPHSKSMPLGRALASTRLDSVSDSRLYDNSPLRKRKTYSAQDGLGSWDRPGYGMDTYGYRQTYSLPSNPTPPCYEQFPFQSLPEQPDQPWQIPYCGMPQDLPRYQDSREKVYINLCNIFPPDLVRIVMKRNPHMVDAQQLAAAILVEKSQLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ36</accession>
    <name>A0A6I9XZ36_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106547076</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919587.1">
        <property type="nucleotide sequence ID" value="XM_014064112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ36"/>
    <dbReference type="GeneID" id="106547076"/>
    <dbReference type="KEGG" id="tsr:106547076"/>
    <dbReference type="OrthoDB" id="6151005at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15227">
        <property type="entry name" value="7tmA_OR14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000037">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="37"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013919587.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="34912" checksum="DA77307C057DAE97" modified="2020-10-07" version="1">MDNNTSIFLLLELSNIREFQIIYASVFLILYLITLTGNFLIISAIIFDSHLHTPMYLFLMNLALQNIGSVSVIIPKSIFNSFMNRRWISYSECVTQVLLFLFFVDSDISLLTVMAFDRYVAICNPLQYEMIMNRKACTQMIGSVWIASLFNAILNTTGTFLTPFCSNIINQFYCEIPHLLKIACSDLYTTEIGVVVFSITLAFGCFVFIVFTYVEIFLAVLRIPSVQGRKKALSTCLPHLIVFSIFLFTGCFAYLRPISKKPQHSDFAITVMYSIIPPMMNPLIYSLRNNDINIALSRIFALRSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZM8</accession>
    <name>A0A6I9XZM8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vasohibin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">VASH1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919867.1">
        <property type="nucleotide sequence ID" value="XM_014064392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZM8"/>
    <dbReference type="GeneID" id="106547279"/>
    <dbReference type="KEGG" id="tsr:106547279"/>
    <dbReference type="CTD" id="22846"/>
    <dbReference type="OrthoDB" id="9974232at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045765">
        <property type="term" value="P:regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028131">
        <property type="entry name" value="VASH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15750:SF5">
        <property type="entry name" value="TUBULINYL-TYR CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15750">
        <property type="entry name" value="VASOHIBIN-1-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14822">
        <property type="entry name" value="Vasohibin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR628131-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919867.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="40190" checksum="9A56FC4C804107A1" modified="2020-10-07" version="1">MSGCKVDSGGGGAAVLQRSQAREGVAQREEEQEEDGDEDLRDGGVPFFVNRGGLPINEATWERMWKHVAKIHPEGELVAMRIRGATDLPKISIPSVPTLQSTTLIPERLEAIQRYIRELQYNHTGTQFFEIKKSRPLTGLMDLAKEMTKEALPIKCLEAVILGIYLTNHMPTLDRFPISFKTYFSGNYFHHIVLGVNIGGRYGALGISRREDLMYKAPTFRTLSELIFDYEEAYRCCWHTLKKVKLGHCVSHDPHSVEQIEWKHSVLDVEKLSREDFRKELERHSRDMRLKISKATGPPSPTKDRKKDVSSPQRSQSSPHRRNSRTERRPSGDKKPTEPKAMPDLNGYQIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0C0</accession>
    <name>A0A6I9Y0C0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Trimeric intracellular cation channel type B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TMEM38B</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Intracellular monovalent cation channel required for maintenance of rapid intracellular calcium release. Acts as a potassium counter-ion channel that functions in synchronization with calcium release from intracellular stores. Activated by increased cytosolic Ca(2+) levels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotrimer; conformation seems to be controled by binding to diacylglycerol (DAG).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM38 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916368.1">
        <property type="nucleotide sequence ID" value="XM_014060893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0C0"/>
    <dbReference type="GeneID" id="106544581"/>
    <dbReference type="KEGG" id="tsr:106544581"/>
    <dbReference type="CTD" id="55151"/>
    <dbReference type="OrthoDB" id="195817at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007866">
        <property type="entry name" value="TRIC_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12454">
        <property type="entry name" value="TRIMERIC INTRACELLULAR CATION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12454:SF5">
        <property type="entry name" value="TRIMERIC INTRACELLULAR CATION CHANNEL TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05197">
        <property type="entry name" value="TRIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013916368.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="31969" checksum="A35465B03406D8F5" modified="2020-10-07" version="1">MELLGDLPLRFARLPMFPFFDLAHYAASIMSLKEQRGAVEVAYDSPLACWFSAMLYCFGGSILSSLMLAEPPIAFLANTTGVLLASSVWYLIFYCPHELAYRCLCFPPIRLMIAGMKEVTRTWKITGGIVHAHKRFADAWLIMIGVGWARGAGGGLISNFEQLVRGIWKPETNELLKMSYPVKVSLIGAVLFTMQCNFYLPITKHNLMLLYTMFLVATKINMMVTGCTTSPFASLEEPLARLFFGLKPTDSPAADEIKGPLNGISSPCDCLLGGEKSEAVEKKQAKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1D3</accession>
    <name>A0A6I9Y1D3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Coxsackievirus and adenovirus receptor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CXADR</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Basolateral cell membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910590.1">
        <property type="nucleotide sequence ID" value="XM_014055115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1D3"/>
    <dbReference type="GeneID" id="106540104"/>
    <dbReference type="KEGG" id="tsr:106540104"/>
    <dbReference type="CTD" id="1525"/>
    <dbReference type="OrthoDB" id="8902063at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034109">
        <property type="term" value="P:homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000095">
        <property type="entry name" value="immunoglobulin superfamily member 11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052307">
        <property type="entry name" value="EJ_Adhesion_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44468:SF3">
        <property type="entry name" value="COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44468">
        <property type="entry name" value="COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026673401" evidence="19">
        <location>
            <begin position="22"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="124"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="277"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013910590.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="39980" checksum="8405145980B1FFE2" modified="2020-10-07" version="1">MASLLPLFWAITFLCADLIEGVTITSTEQTMVTKAQGDKVTLLCTFTLTTADETPVEIEWSIFPPKGEEPVIIYNEGKIYDKYLLMGRVQFAKQNPASGNASIDILNLKTEDTGIYQCKVKKLPSIETHRVHLIVLEKPIKTKCHIEGSQEIGSDLTLKCKSQQGSPIIVYSWTKTTGSQELPATALPNENTGDLFVKNASQTYSGTYTCLANNKVGRDECSVILTITPPIDRAGTIAGAVIGTLLALSLIAFIIFCCYKKRRDTKYEKEVHHDIREDVPPPKSRASTARSYIGSNRSSLGSMSPSNMDGFTKTQYNKVPSEDFERAPSQNQYFDPPKVAAPNLSRMGAIPVMIPAQSKDGSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1E8</accession>
    <name>A0A6I9Y1E8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase-like 26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106547966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0585 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920727.1">
        <property type="nucleotide sequence ID" value="XM_014065252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1E8"/>
    <dbReference type="GeneID" id="106547966"/>
    <dbReference type="KEGG" id="tsr:106547966"/>
    <dbReference type="CTD" id="84326"/>
    <dbReference type="OrthoDB" id="10258744at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010342">
        <property type="entry name" value="DUF938"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20974:SF2">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20974">
        <property type="entry name" value="UPF0585 PROTEIN CG18661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06080">
        <property type="entry name" value="DUF938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920727.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="22755" checksum="3B0EF132527A9163" modified="2020-10-07" version="1">MSRHGSGSAADRNKEPILAVLRAYVGTGHRLQVLEVASGCGLHAAHFAQALPNLEWQPSEVDPQALASISAFIQTLRLSNVKPPIYLDSSQSWETWGGLRPNSLDLIVNINMIHISEMKCTQGLFQGAGKLLKPKAVMITYGPYAVNGFITPQSNVDFDYRLRRSNPAWGLRDTTVLQQLAEANGMQLEDMVDMPANNKCLIFRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1H7</accession>
    <name>A0A6I9Y1H7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Signal peptide peptidase-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SPPL3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920767.1">
        <property type="nucleotide sequence ID" value="XM_014065292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1H7"/>
    <dbReference type="GeneID" id="106547995"/>
    <dbReference type="KEGG" id="tsr:106547995"/>
    <dbReference type="CTD" id="121665"/>
    <dbReference type="OrthoDB" id="29661at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF22">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013920767.1"/>
        </source>
    </evidence>
    <sequence length="347" mass="38015" checksum="79E422AC522B413C" modified="2020-10-07" version="1">MDFENQDKEKDSSNSAGPFNGGSTNNSIQTIDSTQALFLPIGASVSLLVMFFFFDSVQVVFTICTAVLATIAFAFLLLPMCQYLTRPCSPQNKISFGCCGRFTAAELLSFSLSVMLVLIWVLTGHWLLMDALAMGLCVAMIAFVRLPSLKVSCLLLSGLLIYDVFWVFFSAYIFNSNVMVKVATQPADNPLDVLSRKLHLGPSVGRDVPRLSLPGKLVFPSSTGSHFSMLGIGDIVMPGLLLCFVLRYDNYKKQANGDSCGTAGPGNISGRMQKVSYFHCTLIGYFVGLLTATVASRIHRAAQPALLYLVPFTLLPLLTMAYLKGDLRRMWSEPFHSKSSSSRFLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2F2</accession>
    <name>A0A6I9Y2F2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-pyruvoyltetrahydropterin synthase</fullName>
            <ecNumber evidence="4">4.2.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PTS</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTPS family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013920706.1">
        <property type="nucleotide sequence ID" value="XM_014065231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2F2"/>
    <dbReference type="GeneID" id="106547944"/>
    <dbReference type="KEGG" id="tsr:106547944"/>
    <dbReference type="CTD" id="5805"/>
    <dbReference type="OrthoDB" id="14045at2759"/>
    <dbReference type="UniPathway" id="UPA00849">
        <property type="reaction ID" value="UER00819"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003874">
        <property type="term" value="F:6-pyruvoyltetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013920706.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="17132" checksum="DC8C1A0E81DDAFE5" modified="2020-10-07" version="1">MNITYKHVAPVPKLQAWCQKGFCCKQYFSSSPLSFSKHLSKDENMDIFGKCNHTHGHNYQDLMPLNVENVHLGGGNKTPFLCFCPQMAIIEPLDHKDLDNDVDYFANVTSSTENLAMYIWNSLEKILPSGCLYKVKIYESDKNFVVFKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3D3</accession>
    <name>A0A6I9Y3D3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">WNT6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911475.1">
        <property type="nucleotide sequence ID" value="XM_014056000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3D3"/>
    <dbReference type="GeneID" id="106540757"/>
    <dbReference type="KEGG" id="tsr:106540757"/>
    <dbReference type="CTD" id="7475"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19338">
        <property type="entry name" value="Wnt_Wnt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009143">
        <property type="entry name" value="Wnt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF72">
        <property type="entry name" value="PROTEIN WNT-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013911475.1"/>
        </source>
    </evidence>
    <sequence length="320" mass="35827" checksum="5100967D66594B1A" modified="2020-10-07" version="1">MDPNSICRKTKRLAGKQAELCQTEPEIVQEVAKGARLGVRECQYQFRFRRWNCTSHSKYFGKILQQDIRETAFVYAITAAGVSHAVTQACSMGDLLQCGCEATRSRAPPLPPAIMGSESSAWEWGGCGDDVDFGYEKSRQFMDAKRKRGKSDIRTLIDLHNNEAGRLAVKNHMRTECKCHGLSGSCALRTCWKKMPPFREVGDRLLERFNGAFKVMGGNDGKTLIPVGRNMKPPDQQDLIYSADSPDFCIANRKTGSLGTRGRVCNSTAMDMSGCDLLCCGRGHREETLLLEENCLCRFHWCCVVQCRKCTVRKELSLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5A2</accession>
    <name>A0A6I9Y5A2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KAT8 regulatory NSL complex subunit 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106547106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013919633.1">
        <property type="nucleotide sequence ID" value="XM_014064158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5A2"/>
    <dbReference type="GeneID" id="106547106"/>
    <dbReference type="KEGG" id="tsr:106547106"/>
    <dbReference type="OrthoDB" id="6022640at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035035">
        <property type="term" value="F:histone acetyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026180">
        <property type="entry name" value="NSL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22443:SF17">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22443">
        <property type="entry name" value="NON-SPECIFIC LETHAL 1, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013919633.1"/>
        </source>
    </evidence>
    <sequence length="270" mass="29144" checksum="AF567063189A6D6F" modified="2020-10-07" version="1">MERAAIICRWTWLQAQVLDLECRIRQQTDVYKQLRANKGPVILGNLQQEDGLKPPSHLASPAGVMNSRKNPALLPSSPHLKVPSGISPCIPSYLLKNAEKQNSHLAQSLRNLVCQNPSCAPMNGSPEPLKACPSPPQVNGISNCLSACATSGSSAEGPAAEQHLKKPTAANHSLPAVALALDNSCVAARIRPICRYKKRRLVRINSIAHLNQKPPKPLSLKCTCEQPNSCVLCGFKASLPTVDPEATSLEERIALLDSGFHPILSFPHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6M3</accession>
    <name>A0A6I9Y6M3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein eyes shut homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106548035</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920813.1">
        <property type="nucleotide sequence ID" value="XM_014065338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6M3"/>
    <dbReference type="GeneID" id="106548035"/>
    <dbReference type="KEGG" id="tsr:106548035"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF35">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027089879" evidence="7">
        <location>
            <begin position="22"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="174"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="217"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="9">
        <location>
            <begin position="288"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013920813.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="47172" checksum="8FB55AE89292F425" modified="2020-10-07" version="1">MKLQSFVVTIALLLQLSMVGGQTLCNRSLVSEWKTQPTVHTVKWSLSQDICSSFFTECWNIDPYHGSSTAGNVPLRLSQICPLQLQLGDLLFVSPEPSFQSYGMNLANVSLEGFIRCPQPRDVHRHQLLFDHGLSGMRQIAPKWLGVGTHYFAEVPIRGPLLCHFGLRLNVTVKSQACQKFSNSTFCSGHGKCLSRIWDGAYHCHCSHPYSGQFCQEIDKCSSKPCHNSGSCISRKSQREDVEDSYECICPASFTEYEIFSGAQRLAEELAVESIKIIEVEAAIGALYLFNPVARNWTKAKEFCETEKSTLVCIESTLEEMFVTREIAKHPKDFWMGHNYYNDKWECNGIETKIAYWSVATVTNYFKDRCTILLSRCLTTPNCWTNVDCSLDYFTICERKPDNKWHVENAAPVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7G2</accession>
    <name>A0A6I9Y7G2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cordon-bleu protein-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106543592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915120.1">
        <property type="nucleotide sequence ID" value="XM_014059645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7G2"/>
    <dbReference type="GeneID" id="106543592"/>
    <dbReference type="KEGG" id="tsr:106543592"/>
    <dbReference type="OrthoDB" id="8882621at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039895">
        <property type="entry name" value="COBL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21557">
        <property type="entry name" value="CORDON-BLEU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21557:SF2">
        <property type="entry name" value="CORDON-BLEU PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013915120.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12493" checksum="406BDF14198B1BB4" modified="2020-10-07" version="1">MDQKENAIDRDIQLSVILPGEVIKTTNINGSKPMMDLLVYLCAQYHLNPASHTIDLMSPDKKPIKFKPNTPIGMLEVDQVILKPKQMDKKKPIPIIPEQTVRVVVNYKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9N0</accession>
    <name>A0A6I9Y9N0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-directed RNA polymerase III subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">POLR2L</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918960.1">
        <property type="nucleotide sequence ID" value="XM_014063485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9N0"/>
    <dbReference type="GeneID" id="106546586"/>
    <dbReference type="KEGG" id="tsr:106546586"/>
    <dbReference type="CTD" id="5441"/>
    <dbReference type="OrthoDB" id="10258858at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013918960.1"/>
        </source>
    </evidence>
    <sequence length="67" mass="7647" checksum="D0948DF667FE69CE" modified="2020-10-07" version="1">MIIPVRCFTCGKVVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLSHVDLIEKLLNYAPLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA77</accession>
    <name>A0A6I9YA77_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">InaD-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106547340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919945.1">
        <property type="nucleotide sequence ID" value="XM_014064470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA77"/>
    <dbReference type="GeneID" id="106547340"/>
    <dbReference type="KEGG" id="tsr:106547340"/>
    <dbReference type="OrthoDB" id="6022711at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06673">
        <property type="entry name" value="PDZ10_MUPP1-PDZ8_PATJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06674">
        <property type="entry name" value="PDZ11_MUPP1-PDZ9_PATJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06675">
        <property type="entry name" value="PDZ12_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06676">
        <property type="entry name" value="PDZ13_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06671">
        <property type="entry name" value="PDZ7_MUPP1-PD6_PATJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06672">
        <property type="entry name" value="PDZ8_MUPP1-PDZ7_PATJ-PDZ2_INAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000038">
        <property type="entry name" value="Multiple PDZ domain protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000051">
        <property type="entry name" value="Multiple PDZ domain protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000058">
        <property type="entry name" value="multiple PDZ domain protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051342">
        <property type="entry name" value="PDZ_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964:SF11">
        <property type="entry name" value="INAD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964">
        <property type="entry name" value="MULTIPLE PDZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="89"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="263"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="453"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="549"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="694"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="816"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="636"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="640"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013919945.1"/>
        </source>
    </evidence>
    <sequence length="899" mass="97896" checksum="F2A80A3227F6AC52" modified="2020-10-07" version="1">MLLSLLYYYFQCIFLFVNRVLKIYCAICQSLQFSHMSQNIKRPVLGIIMISFDLEQSLFICREIPELPEREEGEGEETPIFSHWGPPQIIEIFREPHVPLGISIVGGHTVIKRLKNGEELKGIFIKQVLEESPAGKTKALKTGDKILEVSGTDLQNATHEEAVEAIKHAGNPIIFVVQSLSTLPMIVSVINPKKKKDDKDQDTEETEKKSGIPPPMKLPPPYKGARKSSSDEPEEEEDEEEENAFTEEKIRQRYAELPGELHIIELEKDKNGLGLSLAGNKDRSLMSIFVVGISPDGPAGKDGRMHIGDELLEINNQILYGRSHQNASAIIKTAPSKVKLVFIRNEDAFNQMAVAPFPLPSCTQSSNEDISSKAEKQVIEDQVKADQPPENLRNQLTQTKFSTVNPIPINLHEISLAPESSYPPETEFSNQNNFPPPPVDPAMCPIVPGQEMTIEISKGRSGLGLSIVGGKDTPLDAIVIHEVYEEGAAARDGRLWAGDQILEVNGMDLRNASHEDAITALRQTPPKVRLVVYRDEAHYKDEENLEVFLVDLQRKMGRGLGLSIVGKRNGSGVFISDIVKGGAADLDGRLIQGDQILSVNGEDVRHASQEVVATILKCAQGLVQLEIGRLRAGSSLSSRKTSQNSQMSQHNVPSHFHPTFAPVISTLQNLVSAKRSSADPSQRNSVGADVSPRTVEITRGLNDALGISIAGGKGSPLADIPIFIAMIQANGVAARTHKLRVGDRIVSINGQPLDGLSHAEVVNLLKNAYGTIILQVVADTNISAIASQIENMTSSTNFTPPTEHHSEYAEPPQPKIIVLEKGSDGLGFSIVGGFGSPQGDLPIYVKTIFGKGAAADDGRLKRGDQILAVNGQSLEGVTHEQAVTILKHQKGEVTLTVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBI4</accession>
    <name>A0A6I9YBI4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TBC1 domain family member 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TBC1D19</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921477.1">
        <property type="nucleotide sequence ID" value="XM_014066002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBI4"/>
    <dbReference type="GeneID" id="106548602"/>
    <dbReference type="KEGG" id="tsr:106548602"/>
    <dbReference type="CTD" id="55296"/>
    <dbReference type="OrthoDB" id="10249775at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042507">
        <property type="entry name" value="TBC1D19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16110">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16110:SF1">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013921477.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="39007" checksum="6EEFD9810B572C21" modified="2020-10-07" version="1">LTIAQIVERLKGSGLHAQLERQARLSLHNPEIKLQSLKEDIKEYLKTSGWEKKLHNAVYREISMFPLPYSPSVPPEHIKEPLAYMRKAQANWEKRILKSLNSMCIELGIPLSQKRPLSEQKELLNKWNEMGTDEPDLSLFRPVYAPKDFLEVLMNLRNPNYDTSEVPNFRNHLGLIQVPLNVKDIPELVSIHQLYENEHVRLGQKVLMEQDSAAAQQYVRQGSPNSLRAELWALILNISTRPEVSQKSRLEGQGSACYSSDILNSPRFIQPKISIVKQDSYEDVKLTASNDDYYFVFEDYLYQVLLCFSRDTSVLEHFEYNSATPPRSFIRGKDRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCK8</accession>
    <name>A0A6I9YCK8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger protein 618</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ZNF618</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="543" mass="60361" checksum="F60597F754492A2B" modified="2020-10-07" version="1">MQSHAADNENNIASNQPRSPPAVGEEKWKPQPQRNNSNNTSAGSSLLNSPIPEKERQNIAERLLRVMCADLGTLSVVSGKEFVKLAQSLVDNGARYGAFSVTEILGNFSTLALKHLPRMYNQVKVKVTCALGSNACLGIGVTCHSQSVGPDSCYILTAYQVEGSCIKSYVLGIKGVDIRDSGDLVHHWVQNVLSEFVMSEIRTVYVTDCKVNSSAFSKAGMCLRCSACALTSVVQSVLNKRTLQARNMHEVIELLNVCEDLAGSAGISKETFGSLEETSPPPCWNSITDSLLLVHERYEQICEFYSRAKKLNLIQNLNKHLLSNLAAILAPVKQAVIELSNESRPTLQLVLPTYVKLEKLFTSKANDAGIVSKLCHLFLEALKENFKVHSAHKVAMILDPQQKLRPVPPYQHEEIIGKVCELIHEVKESWAEEPDFEPASKKSRTPSESSPTREEDRSGKSEVYDYLQEPLFQAAPDLFQYWSCVTQKHTKLARLAFWLLAVPAVGARSNCVNMCEQALLIKRRRLLSPEDMNKLMFLKSNML</sequence>
</entry>
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    <accession>A0A6I9YCR0</accession>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_013921938.1"/>
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    <sequence length="149" mass="16938" checksum="772CC8CAB8C37F41" modified="2020-10-07" version="1">MFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMIKEASGPINFTVFLTMFGEKLKGADPEDVIIGAFKVLDPEGKGNVKKKFLEELLTTQCDRFTPEEIKNMWHAFPPDVAGNVDYKNICYVITHGEDKEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDF7</accession>
    <name>A0A6I9YDF7_9SAUR</name>
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        <submittedName>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="84"/>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
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        </source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="234" mass="25704" checksum="58D6E72AE4F8364E" modified="2020-10-07" version="1">MVTAPKMLLGLLKGYDSIPIPACALQMFIFAAVSDAECCLLAVMAYDRYVAICRPLHYATAMPQHLCHLLVIASYAAGMTSAAIHSSMAFRLPFCRANTLDHFFCDIPLVLALACADTQINQILLLVVCGTIQSITLLTITTSYGIILWTVGRTGSFRAMSTCGSHLIVVGMLYGTLLFMYLRPDDTYAPQTDKITSVFYTVAIPTLNPAIYSLRNTEVKQAVKKRFSKICHQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDI0</accession>
    <name>A0A6I9YDI0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Nucleoplasmin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">NPM3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoplasmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922218.1">
        <property type="nucleotide sequence ID" value="XM_014066743.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922226.1">
        <property type="nucleotide sequence ID" value="XM_014066751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDI0"/>
    <dbReference type="GeneID" id="106549164"/>
    <dbReference type="KEGG" id="tsr:106549164"/>
    <dbReference type="CTD" id="10360"/>
    <dbReference type="OrthoDB" id="9900353at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.340:FF:000011">
        <property type="entry name" value="Nucleophosmin/nucleoplasmin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004301">
        <property type="entry name" value="Nucleoplasmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024057">
        <property type="entry name" value="Nucleoplasmin_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036824">
        <property type="entry name" value="Nucleoplasmin_core_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22747">
        <property type="entry name" value="NUCLEOPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22747:SF13">
        <property type="entry name" value="NUCLEOPLASMIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03066">
        <property type="entry name" value="Nucleoplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69203">
        <property type="entry name" value="Nucleoplasmin-like core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin core" evidence="5">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="131"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013922218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013922226.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18617" checksum="B717DCA60A5826F7" modified="2020-10-07" version="1">MCIPQGSMQVSLHSNSCWSNCIARLSNIGCELNSSARSYTFKVSEEDDSEHFLALNMACLSAGAKEECNIVEVVGHDYQNKEVAVPVANLKLSCQPWLCLDNFEFQPPVTFRLRSGSGPVHLSGQHQIFHRKDLSDEDVSDDDDDDESIDQEEELSPIKPAKKKQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDJ6</accession>
    <name>A0A6I9YDJ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AADAT</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_013922114.1">
        <property type="nucleotide sequence ID" value="XM_014066639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDJ6"/>
    <dbReference type="GeneID" id="106549085"/>
    <dbReference type="KEGG" id="tsr:106549085"/>
    <dbReference type="CTD" id="51166"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000071">
        <property type="entry name" value="Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="5">
        <location>
            <begin position="43"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013922114.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="38955" checksum="05FEEDC477DD9C35" modified="2020-10-07" version="1">MNYSRFITAVSAARKASPIRVLTELMQQSPTSLISLAGGAPNSNTFPFKSATIIIKDETRVEIKEELMRRALQYSASAGIPELLSWLKDMQKTIHNPPTVDYSPKEGQMEMCITTGSQEGLCKVFEMLINPGDNILLDAPTYPGTLAALRPLGCNIINVPSDQHGMIPKALKEVLSRWNPEDVKKLKNDTPKFLYTIPNGGNPTGASLTTERKKEIYQLARQYDFLIIEDDPYYFLQFNKPWAPTFLSMDIDGRVIRTDSFSKILSSGLRIGFLTGPKPLIDRVILHIQVSTMHTSTFTQLIIAQLLQQWGLKGFLDHIDSVVDFYKQQRDVMLNAADKWLKGWSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDK3</accession>
    <name>A0A6I9YDK3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reactive oxygen species modulator 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein MGR2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ROMO1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Has antibacterial activity against a variety of bacteria including S.aureus, P.aeruginosa and M.tuberculosis. Acts by inducing bacterial membrane breakage.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922100.1">
        <property type="nucleotide sequence ID" value="XM_014066625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDK3"/>
    <dbReference type="GeneID" id="106549070"/>
    <dbReference type="KEGG" id="tsr:106549070"/>
    <dbReference type="CTD" id="140823"/>
    <dbReference type="OrthoDB" id="5409308at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018450">
        <property type="entry name" value="Romo1/Mgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525:SF1">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10247">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01378">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013922100.1"/>
        </source>
    </evidence>
    <sequence length="83" mass="8757" checksum="DF66A49DC6946AD9" modified="2020-10-07" version="1">MVFKMPVTVGSYGQSQPSCFDRVKMGFMMGFAVGMAAGALFGTFSCVRIGMRGRELMGGVGKTMMQSGGTFGTFMAIGMGIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDL7</accession>
    <name>A0A6I9YDL7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Keratin, type I cytoskeletal 18-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106549201</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922258.1">
        <property type="nucleotide sequence ID" value="XM_014066783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDL7"/>
    <dbReference type="GeneID" id="106549201"/>
    <dbReference type="KEGG" id="tsr:106549201"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF358">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="4">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="98"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013922258.1"/>
        </source>
    </evidence>
    <sequence length="287" mass="32881" checksum="E95EE327D0B99DC8" modified="2020-10-07" version="1">MENVRLLLQIDNARLATDDFKHKLEVEQAACDTVQKDTQGLRKIIDDTNFLRLKLEGDLDSLGEELAHLRKCHEEEVEALTPLVANSDVTVQVDNPQKNDLSETIAEIRNQYEQVAEQNRADTQDWYRTKVDTMSQEASMNTQALEQARNELSDLRRQLQGLEIERQTLQKMVDALENTLKNTEDHYLSGLANLNQIIAKLQEDLAACQADLETQSRDYEILLDLKTKLENEIEHYRVLIEGVLDRGDVLSSGKAEIRSQRIKKVVVFTHKIVNGQDVTQRSEEITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFB4</accession>
    <name>A0A6I9YFB4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Armadillo repeat-containing protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARMC3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922923.1">
        <property type="nucleotide sequence ID" value="XM_014067448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFB4"/>
    <dbReference type="GeneID" id="106549703"/>
    <dbReference type="KEGG" id="tsr:106549703"/>
    <dbReference type="CTD" id="219681"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052441">
        <property type="entry name" value="Armadillo-Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46618">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46618:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="150"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="5">
        <location>
            <begin position="287"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="480"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="EDR1/CTR1/ARMC3-like peptidase-like" evidence="4">
        <location>
            <begin position="712"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="622"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013922923.1"/>
        </source>
    </evidence>
    <sequence length="861" mass="94901" checksum="36E6FAABAE158E93" modified="2020-10-07" version="1">MGKKVKKEVEPPPKDVFDPLSVESKKAATVVLMLSSPEEEVLYKGCEALYKFALKGEENKLTLLELGALEPLFKLVIHEDPTVRRNATMVVGIMASHNDVKKSLRQLDVTSAFIARLAPEEDVVIHEFASLCLVHMSIDYNSKVQIFEQGGLEPLIRLLGSPDPDVKKNCVECIYNLVQDFQSRAAIRELNVMPPLLELLKSDYPVIQLLALKTLGAITIDKETRMMLRENQGLDHLFKILETKEFNDLHVEALGVVANCLEDVDTMQLIQETGGLRKLLAFTEVSTLTEFQKNAAKAIAKAAADPENRKILNEQEVEKCLITLLGTDNEGTKIAASQAISAMCENLASKQTTGALGIPQLVQLLSSENEEVKEAAATALVNLTTAHPANVSAVAEAEAIEPLISVLSCKRDGAVASAASVLTNLAMQEPLRLTIQSHGFMTAIVEPLNSTNSIVQSRAALAVAAMGCDTDGRTELRNSGGFEPLVKLLHSKNDEVRRNACWAVMVCANDELTAAEMTRLGALDILDEINLSIGRKNNFSEAALEKILDHNLPQKYSQMGFLASTNIILDGFYDYGQVKPGEKLLSLEELCGQELNDHRAIILINAKLSELEHQAISISIEEKPQENPASRIGASATSVRKTSRNNSLDEKPESPGRSSSISKGSSREKGSRKGKGKKEEEKPKDEELEASIPKVVETPGKALWIPPFDPILFDYITEISRTILPLPTSKEQVVALAQFVADRLGGPIERNNLHEFSWELHISEIEYELKCNVVPIGKIEKGTFYHRALLFKVLADRIAINCSLYRGEYNRAWNEVKLVDDSPLGIPGLLLPPQVYIVDLMYQPGCLMKEESPEADCYRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFK3</accession>
    <name>A0A6I9YFK3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitogen-activated protein kinase kinase kinase 12</fullName>
            <ecNumber evidence="14">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MAP3K12</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">May be an activator of the JNK/SAPK pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013922966.1">
        <property type="nucleotide sequence ID" value="XM_014067491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFK3"/>
    <dbReference type="GeneID" id="106549757"/>
    <dbReference type="KEGG" id="tsr:106549757"/>
    <dbReference type="CTD" id="7786"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14059">
        <property type="entry name" value="STKc_MAP3K12_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000087">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000095">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017419">
        <property type="entry name" value="MAP3K12_MAP3K13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027257">
        <property type="entry name" value="MAPKKK12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF17">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500741">
        <property type="entry name" value="MAPKKK12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038165">
        <property type="entry name" value="MAPKKK12_MAPKKK13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="165"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="52"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="563"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="625"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="837"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="459"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="576"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="665"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="736"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="763"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="848"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="171"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038165-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038165-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013922966.1"/>
        </source>
    </evidence>
    <sequence length="877" mass="96625" checksum="EA4EC583C3D2DDAA" modified="2020-10-07" version="1">MACLHETRTPSPSFGSFNTILTENSLRKLDPDASDCTPEKDLTPTQCVLRDVLPIDGSGGGGGGHSPTPSEETHEQFANSVLQLHENESGSGGGGAGAGAGVGNPEVRPTRYHADQVRIHCQTGSGFLEGLFGCLKPVWTMIGKAYSTEHKQQQEDPWEVPFEEIMDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCIIMEFCAQGQLYEVLRAGRKVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPTSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELIHRRREELRHALDIREHYERKLERANNLYMELNSLMLQLELKEKELLRREQTLEKKYPGLFKHHPSRSLLHGNTVEKLIKKRNVPQKLSPHSKRPDILKTEVILDPSLPQLAIPVCQKGPPSPGRSRRAKSRYRKVSTRGSCGELADPGGAKRLEPCTALRGLQHDLLLRKMSSSSPDLISTTLGAEGRKGGTGPESPPTEQPRSETPSEDTASVPCSSSPDSPSSRQGAVGPPGKARLQPGEEAEREQGKGSRSGAPQQSQHLTPAAMLYRAAVTRSQKRGISSEEEEGEVDSEVELPLRQRWPQARSKRQSLSTFSSENFSDGDGEEGTGSEGTTASHSGGTPDVPSTNTDERLDAERSDDDILGNSDGLSEKEAAIQLVKRQLSAEQNAGEDPAAYEDSDCDSAELDNSGSGEVQRLPDIPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFV2</accession>
    <name>A0A6I9YFV2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Complement C1q and tumor necrosis factor-related protein 9A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106549847</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923076.1">
        <property type="nucleotide sequence ID" value="XM_014067601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFV2"/>
    <dbReference type="GeneID" id="106549847"/>
    <dbReference type="KEGG" id="tsr:106549847"/>
    <dbReference type="OrthoDB" id="5875591at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF21">
        <property type="entry name" value="COMPLEMENT C1Q AND TUMOR NECROSIS FACTOR-RELATED PROTEIN 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C1q" evidence="6">
        <location>
            <begin position="133"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013923076.1"/>
        </source>
    </evidence>
    <sequence length="277" mass="29355" checksum="9A2FC18C3A3C7A03" modified="2020-10-07" version="1">MIHEATVEQVIHGADGRVDEKGNKGDKGQRGWPGKFGPKGVPGPVGDKGQQGELGPQGRKGIKGDLGPIGPKGQKGEIGIQGEIGLRGPIGPKGHVGPKGEIGGPGPKGNKGNQGERGWKGAQGEKGNLGSTPVIARSAFSVGLTAKFPPLNVPIKFDKVLYNGFNHYSTETGLFTCQIPGVYYFTYQITVFSRNVRVALVKNEHRMLHTADMYQGSEDQASGGIVLELQKGDRIWLQTYGGETFNGLFADPDDDTVFSGFLLFSTSEAPASLPSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG11</accession>
    <name>A0A6I9YG11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thyrotropin-releasing hormone receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thyroliberin receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106549739</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor for thyrotropin-releasing hormone (TRH). Upon ligand binding, this G-protein-coupled receptor triggers activation of the phosphatidylinositol (IP3)-calcium-protein kinase C (PKC) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922934.1">
        <property type="nucleotide sequence ID" value="XM_014067459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG11"/>
    <dbReference type="GeneID" id="106549739"/>
    <dbReference type="KEGG" id="tsr:106549739"/>
    <dbReference type="OrthoDB" id="10036964at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004997">
        <property type="term" value="F:thyrotropin-releasing hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002120">
        <property type="entry name" value="TRH_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46061">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46061:SF5">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00751">
        <property type="entry name" value="THYROLIBRINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01846">
        <property type="entry name" value="TRHRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="29"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013922934.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="33339" checksum="5A5E43652C0CC2C1" modified="2020-10-07" version="1">MPLGATLLHEAGSDPDKETSVRVLARLDQRYIAICHPMKAQTMCTVSRAKRIAAFVWLLTSLYCMLWFFLVDVAANNSQQLECGYKVSRNLYLPIYLLDFALFFIAPLLLTTVLYGLIGKALLQSHALHRPTAQGECWRERSNQEKNGTEAGSSRTRVPVSSRKQVTKMLAVVVALFALLWMPYRTLVLVNSFVKHPYLDRWFVLFCRICIYTNSAINPIIYNLMSQKFRLAFKRLCKYREEELQRQRSLCTADGSTRKAGPTGQQGSLAGTPTPAAAQETLSQKEKEMQFSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGI3</accession>
    <name>A0A6I9YGI3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox protein Hox-A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HOXA1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Antp homeobox family. Labial subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923287.1">
        <property type="nucleotide sequence ID" value="XM_014067812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGI3"/>
    <dbReference type="GeneID" id="106550030"/>
    <dbReference type="KEGG" id="tsr:106550030"/>
    <dbReference type="CTD" id="3198"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000113">
        <property type="entry name" value="homeobox protein Hox-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046327">
        <property type="entry name" value="HXA1/B1/D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946">
        <property type="entry name" value="HOMEOBOX PROTEIN ROUGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="227"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="229"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="281"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923287.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="36788" checksum="FE0C868EFBEAF239" modified="2020-10-07" version="1">MDTARMNSFLDYPPILNGEPTTCASRAYHRHHHHHHPDHGITTTFQSCAVSTNSCNGDDRFLVGRGVQISPHPHHHQAGTTYQHHPNLGMSFSHPSCGSGYGAQNFTAGGYGHYPLSQEAEITGGYSPCAPTLYSGNLSSSMVQHPQSYGGGTTSSTQYIHPSFGEEQQNLTLTNFSHPLSPLHSAHPESCTSPSSEVSPPAQTFDWMKVKRNPPKTGKAGEYGYVGQPNTVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNRRMKQKKREKEGLLPISPATPTGSEEKAEDSSEKSSSSPCTPSPTSSTSDTLTTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGY0</accession>
    <name>A0A6I9YGY0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Patched domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923441.1">
        <property type="nucleotide sequence ID" value="XM_014067966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGY0"/>
    <dbReference type="GeneID" id="106550151"/>
    <dbReference type="KEGG" id="tsr:106550151"/>
    <dbReference type="OrthoDB" id="429851at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042480">
        <property type="entry name" value="DISP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053954">
        <property type="entry name" value="DUF7023"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46687">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46687:SF1">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22894">
        <property type="entry name" value="DUF7023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="530"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="3">
        <location>
            <begin position="450"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923441.1"/>
        </source>
    </evidence>
    <sequence length="875" mass="97996" checksum="2FDC6E3D5912E3DD" modified="2020-10-07" version="1">MDTEDDALLQDVCGNEDEEGACGPGVKTRGSRLCGKCQWNPRHQATFVTLPSSDFWTVVGWVFTNPYSASLILFLGCAIPVGLALTMFLHYPALDIDISYNAFEIRNHESSERFDALTLALKSQFGLWGRSRRDLADFSSETLRRLIFEKLQHLQRNASRPGSPSLSRRRRRDAGRNQAGPANHTSHQSRSPLPRWEFPSATVSANTQTHAHWRIELIFLARGDAEKNIFTSERLLTIHEVERKIMDHPHFQEFCWKPHEVLKDLPLGSYSYCSPPSSLMTYFFPTERGGKIYYDGLGQELADIQGSLELAMTHPEFYWYVDEGLSAENKKSSLLRSEVLFGAPLPSYYSMDDRWEEQCRKFQNFVVTYVALLAKESTSKVEVLYGGTDLFDYEVRRIFKNDMLLAFISSSCIAVLVYLLASCSVFLSFFGIASIGISCLVALFLYHIVFGIQYLGILNGVAAFVIVGIGVDDVFVFINTYRQATHLKDLNLRMIYTIQTAGKATFFTSLTTAAAYAANIFSQIPAVHDFGLFMSLIVSCCWVGVLFTMPAALGIWSLYWSSLENTCQARCSKKCTKESALQSSDGLFVSLAVAGRSGLGPLPYLDDDIPLLNVEEEPVALEMGDVPLVSVLPENHHLTTNNDSPGHLITHLQKLLQCWALWSAVKNKWVIVGLFVLVLILSIFFASRLRPASRAPLLFRPDTNIQVLMDLKYNLSAEGISCVTCSGLFQEKPRNLQNNIRTSLEKKKRGSGPPGSGRGSSLDDRSLQDPQGTVYIFRSKGSSRPVIYRFSLNATVPAPWQTVPAKDGEVPSFQVYKVPYGNFTKRLTACMSTVGHVQPGASKKWMMTSLSCDTKRGWKSDFSFYVATQEQQRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH26</accession>
    <name>A0A6I9YH26_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transmembrane protein 79</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TMEM79</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923482.1">
        <property type="nucleotide sequence ID" value="XM_014068007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH26"/>
    <dbReference type="GeneID" id="106550178"/>
    <dbReference type="KEGG" id="tsr:106550178"/>
    <dbReference type="CTD" id="84283"/>
    <dbReference type="OrthoDB" id="8887147at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000026">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF12">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013923482.1"/>
        </source>
    </evidence>
    <sequence length="765" mass="83337" checksum="E65E603BD9E96911" modified="2020-10-07" version="1">MSAIAPSEDMAMVPLGKDTSPAAQLTQTHTQKPSSEDTEATLPWDRAQHDTVGEMPGVLETDARPSPEGSMKAAMDHQAFLKPLEDEECSGMSAMAAHAFIPIDPLCIERRPRSHKKQPWQLDVPLQDRAPYQEEKADPEQQTFLARDSSACSHDLPDPAGEPSQTHCCRTKGCCNSANLKAVASMTTALIIFPGLVYSAYVFLPFDMPVMESMSARLVYTLRCGVFATFPIILGMMVYGVSRLCASSLQPFEELSREVEIHRHFVSQSVDLFILYFFNIAVLSTYLSQEGLKLIPLLTGLFAISRRRLLDKTNITLTIAIKEARATELAELSTLEMARYLPPAPTLPPVPALPAPTPPPPLPSSPSTCLIDELVPLPDSFYEGQVDRLRAIPRRPQPSQARATAESATCLSCGGNHARASCPFKTSVCRRCGKKGHLAQVCRATLPPPADQPLPAYGPPVPPRNQQPRRQQARQDDCYALEEDLPATPAVSVSQASAGMRKITTTISIEGSPCPMEVDSGSSRSLLSWATFSRLCPQVPKSQVSPADTLLRDYQGTNIHTLGCYGVRVQVEFLGFMIDVQGIHPTPSKISAIKNAPVPTSKVQLQAFLGLLNFYAPFLPHKASLAEPLHRLLEQSVAWQWGSREAHAFAAVKSLLTSEAVLVQYSNKMPLTLACDASPVGLGAVLSHVLPNGSEAPIAFYSRTLSSAERNYSQIDKEALAAVSGVKKFHNYLYGRHFTLYTDHKPLLGLFAGDRPTPPPSCLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI17</accession>
    <name>A0A6I9YI17_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mannose-binding protein C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106550176</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923480.1">
        <property type="nucleotide sequence ID" value="XM_014068005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI17"/>
    <dbReference type="GeneID" id="106550176"/>
    <dbReference type="KEGG" id="tsr:106550176"/>
    <dbReference type="OrthoDB" id="10255512at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF42">
        <property type="entry name" value="MANNOSE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0176">Collagen</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026646992" evidence="10">
        <location>
            <begin position="23"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="11">
        <location>
            <begin position="133"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="41"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013923480.1"/>
        </source>
    </evidence>
    <sequence length="247" mass="25849" checksum="7382BF537FF81AA0" modified="2020-10-07" version="1">MFFLQPFNILLLLFLGASLASAAAPDTNSCTVLACGNPGVAGLPGRDGRDGAKGEKGDTGLQVRGQQGFPGKAGPAGPKGNTGAQGEKGQKGEMSAVDTVQRQIAALEKMVQTLQAEVTKSKKIFSMQGVTTIGGKTFVSTGQSDNFANGKALCSNAGGALAAARNVAENTALAAMAKRNGKHIYLGISDIQTEGKFVYLNGQAVGYTKWKRTEPNNLNEEDCVILLPDTQWNDISCDHQTLIVCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIL0</accession>
    <name>A0A6I9YIL0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cullin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CUL3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 13">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923660.1">
        <property type="nucleotide sequence ID" value="XM_014068185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIL0"/>
    <dbReference type="GeneID" id="106550300"/>
    <dbReference type="KEGG" id="tsr:106550300"/>
    <dbReference type="CTD" id="8452"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000091">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000001">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000005">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000006">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000002">
        <property type="entry name" value="cullin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000002">
        <property type="entry name" value="cullin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cullin family profile" evidence="15">
        <location>
            <begin position="383"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="682"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923660.1"/>
        </source>
    </evidence>
    <sequence length="768" mass="89001" checksum="A183DCE59F24F98E" modified="2020-10-07" version="1">MSNLSKGTGSRRDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLACMYKLFSRVPNGLKTMCECMSTYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLQESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIN2</accession>
    <name>A0A6I9YIN2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lysosomal-associated transmembrane protein 4B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LAPTM4B</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homooligomer; upon reaching the lysosomes. Interacts with MCOLN1. Interacts with NEDD4; may play a role in the lysosomal sorting of LAPTM4B; enhances HGS association with NEDD4; mediates inhibition of EGFR degradation. Interacts with PIP5K1C; promotes SNX5 association with LAPTM4B; kinase activity of PIP5K1C is required; interaction is regulated by phosphatidylinositol 4,5-bisphosphate generated by PIP5K1C. Interacts with HGS; promotes HGS ubiquitination. Interacts with SNX5. Interacts with SLC3A2 and SLC7A5; recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation. Interacts with LRRC32; decreases TGFB1 production in regulatory T cells. Interacts with BECN1; competes with EGFR for LAPTM4B binding; regulates EGFR activity. Interacts with EGFR; positively correlates with EGFR activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endosome</location>
            <location evidence="5">Multivesicular body lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LAPTM4/LAPTM5 transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923799.1">
        <property type="nucleotide sequence ID" value="XM_014068324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIN2"/>
    <dbReference type="GeneID" id="106550417"/>
    <dbReference type="KEGG" id="tsr:106550417"/>
    <dbReference type="CTD" id="55353"/>
    <dbReference type="OrthoDB" id="10002163at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004687">
        <property type="entry name" value="LAPTM4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051115">
        <property type="entry name" value="LAPTM_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12479">
        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12479:SF6">
        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03821">
        <property type="entry name" value="Mtp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 21 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00010076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013923799.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="25367" checksum="AFBD1C79A7641895" modified="2020-10-07" version="1">MKMLGSPWTRFYSNSCCLCCHVRTGTIILGIWYLILNAVVLLIILSALTDPGQYHLAGTELSNEFEFMDDANMCIATAVSLLMILICAMATYGAYKQHAAWIIPFFCYQIFDFALNTLVAISILVYPNTVRDYLQQLPANFPYKEDIMSMNSICLIVILLLCLSITLAFKAYLISCVWNCYRYINNRNNSDVLVYITTNDTAVLLPSYDDSANGPGKDAPPPPYAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJD3</accession>
    <name>A0A6I9YJD3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pentraxin family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106550666</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the pentraxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924084.1">
        <property type="nucleotide sequence ID" value="XM_014068609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJD3"/>
    <dbReference type="GeneID" id="106550666"/>
    <dbReference type="KEGG" id="tsr:106550666"/>
    <dbReference type="OrthoDB" id="547680at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001849">
        <property type="term" value="F:complement component C1q complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000070">
        <property type="entry name" value="Serum amyloid P-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051005">
        <property type="entry name" value="Pentraxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869:SF7">
        <property type="entry name" value="C-REACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869">
        <property type="entry name" value="C-REACTIVE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0011">Acute phase</keyword>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pentraxin (PTX)" evidence="11">
        <location>
            <begin position="36"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013924084.1"/>
        </source>
    </evidence>
    <sequence length="235" mass="26571" checksum="F04B013C3A90F866" modified="2020-10-07" version="1">MTQRGPGFLVLVEEEKMALCSPTPNSGGFSQRSDLQKKVFIFPATSPKAAVRLNVNLQKPLTKLSVCLKHHCPLLRPYSLFSYATRAHDNAFLIFKEDHSVYSVSIGNSDVRFKIPTEVIPSWEHICVSWNSRNGLIYFWLNGVLLPRLGTKRGYKISHQASIFLGQDQDTFGGGFQASQSFVGDMAEVNMWPRMLTSDDVRLLMKDDTVPDPIFSWSSFNYTIQDYVVLTEEVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKB0</accession>
    <name>A0A6I9YKB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type phospholipid transporter</fullName>
            <ecNumber evidence="3">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106551101</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013924591.1">
        <property type="nucleotide sequence ID" value="XM_014069116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKB0"/>
    <dbReference type="GeneID" id="106551101"/>
    <dbReference type="KEGG" id="tsr:106551101"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF78">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE IK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="569"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="614"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="658"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="15">
        <location>
            <begin position="535"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="735"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="735"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013924591.1"/>
        </source>
    </evidence>
    <sequence length="768" mass="87970" checksum="7F98D3C934273D3D" modified="2020-10-07" version="1">MEDQDQRRSKCPLLRTEQTKLFWFEFIYLVHSCFINWDTEMYYAEKDTPAKARSTSLNDQLGQIEYIFSDKTGTLTQNIMTFKKCCIHGIIYGKITVTLLLVSFGLAIGSGLWATRFFKEHSYLFAFYRHTSAVSYAFFAFWGFLILLSLVIPMSMLITFVLQTFSQAAFSIDIQSVKLTEEGRSLGYSGEVVCEEPNSRLHNFVGTLEWHGEKYPLDCEKILLRGCRVRNTAVCYGLVIYAGFDTKIMKNCGKIKLKKTKLCHLMDRLVLLDLSWNKYIDKNLEFYDQLLLDTIRRDQDPVVREYMRLLALCHTVMVEEKEMQDPEGCIKLYTKGADSVILERLHSDRTNEDCTVKALDLLGATAIEDKLQNGVPETIHLLKRGGIKVWVLTGDKQETAVNIGFACQLLSEDMTILEESEIRYRVLKEKIFHTVENKKSKEKKGLLEHLRFRIKDTSKEHGYFIEKAFVELATTCQAVICCRVTPKQKALIVQMVKRHKNPISLAIGDGANDVNMIKTADIGVGISGQEGMQAVQCSDYALAQFRYLQRLLFVHGKWSYIRICKFLRYFFFKTFAGMMVQIWFAFYTGFTAQDVNAKKSLQFPELYKAGQKDLFFNFWIFFVSFIHGSFVSLANFYITMWAMGDRSGVRVTGDYQSYGMVVATSAILTVLTEMFQAREMQKKQSTVELTSHNNRNSLLRRSSYAFSHKEGYAHLIAKGASLRSKTVPVRKQLNGLYSTNSPGKDNSLPTEINAAPKEDSLEQKVQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKZ5</accession>
    <name>A0A6I9YKZ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sushi repeat-containing protein SRPX2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SRPX2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924806.1">
        <property type="nucleotide sequence ID" value="XM_014069331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKZ5"/>
    <dbReference type="GeneID" id="106551269"/>
    <dbReference type="KEGG" id="tsr:106551269"/>
    <dbReference type="CTD" id="27286"/>
    <dbReference type="OrthoDB" id="6136178at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090050">
        <property type="term" value="P:positive regulation of cell migration involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000024">
        <property type="entry name" value="Sushi repeat-containing protein SRPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025232">
        <property type="entry name" value="DUF4174"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043555">
        <property type="entry name" value="SRPX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46343">
        <property type="entry name" value="HYR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46343:SF3">
        <property type="entry name" value="SUSHI REPEAT-CONTAINING PROTEIN SRPX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13778">
        <property type="entry name" value="DUF4174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 15" id="KW-0768">Sushi</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Sushi" evidence="17">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="17">
        <location>
            <begin position="67"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="16">
        <location>
            <begin position="124"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="17">
        <location>
            <begin position="209"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013924806.1"/>
        </source>
    </evidence>
    <sequence length="412" mass="47059" checksum="6BAE03E631F3E3E2" modified="2020-10-07" version="1">MVPRWCHPLIIYNGEATCYSPRGGNYRSSLGTRCALSCNRGYRLIGPRSVQCLMNRHWSGIIYCRQVRCYTLPAFQHGSYHCSNGVKIDSHCHYTCQLGYQLEGDRTRVCMEEGRWSGSVPICVDTEPPQIRCPDSRERIAEPGKLTARVYWDPPRAKDSADGIIKKLKRRGPEPGSEFSEGEHLIRYAVYDQAHNRATCKFLVRVRVSRCPTLKAPEHGYLSCSSAGNNYGAICEYQCNGGFELEGTRSRVCQSSRQWSGSQARCTPMQINVDVNSVAGLLDQFYEKRRLLVVSAPDSSDRYYKMQNAMLQQATCGLDMRHMTVIELVGLPPRDVGRIRELQLSEKIVEELRQLLHISHSRFNVVLIDKDGTDRDRYIQPVTPDELFSYVDMYLLSTQEQSQQEQNRDMCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNL7</accession>
    <name>A0A6I9YNL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="13">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 13">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SNRPD2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926078.1">
        <property type="nucleotide sequence ID" value="XM_014070603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNL7"/>
    <dbReference type="SMR" id="A0A6I9YNL7"/>
    <dbReference type="GeneID" id="106552354"/>
    <dbReference type="KEGG" id="tsr:106552354"/>
    <dbReference type="CTD" id="6633"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000069">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13 16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926078.1"/>
        </source>
    </evidence>
    <sequence length="108" mass="12399" checksum="3E92B3D47F961A7F" modified="2020-10-07" version="1">MTPEELQKREEEEFNTGPLSVLTQSVKNNTQVLINCRNNKKLLGRVKAFDRHCNMVLENVKEMWTEVPKSGKGKKKSKPVNKDRYISKMFLRGDSVIVVLRNPLIAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNL8</accession>
    <name>A0A6I9YNL8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Tectonic-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TCTN2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the tectonic-like complex (also named B9 complex).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tectonic family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925751.1">
        <property type="nucleotide sequence ID" value="XM_014070276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNL8"/>
    <dbReference type="GeneID" id="106552087"/>
    <dbReference type="KEGG" id="tsr:106552087"/>
    <dbReference type="CTD" id="79867"/>
    <dbReference type="OrthoDB" id="9282501at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904491">
        <property type="term" value="P:protein localization to ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040354">
        <property type="entry name" value="TCTN1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011677">
        <property type="entry name" value="TCTN1-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057724">
        <property type="entry name" value="TCTN1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611">
        <property type="entry name" value="TECTONIC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611:SF6">
        <property type="entry name" value="TECTONIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25752">
        <property type="entry name" value="DUF1619_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07773">
        <property type="entry name" value="TCTN_DUF1619"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026878212" evidence="7">
        <location>
            <begin position="20"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="655"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3 N-terminal" evidence="9">
        <location>
            <begin position="153"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3" evidence="8">
        <location>
            <begin position="273"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3" evidence="8">
        <location>
            <begin position="419"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925751.1"/>
        </source>
    </evidence>
    <sequence length="681" mass="73836" checksum="A04BF9931C5C3454" modified="2020-10-07" version="1">MSLLRGVLWGLLSLFGAGAQDGQQDFQPSFIHMSGTMVTASLTNSHADTFSLAVADNETGLLPLSDCSGRNRTDDWSLQVTRQGDVSKVTVSLTRHLQLCVSNATDCCAESLCLLQSLQVSACQGAKVMARLLIQAEIYANTSSGNLTGKIEVENATMIPNQVFHPLGSCPCNLTAGACDIQCCCDEDCTPEMKQLFEGSCFEGVFGGDVNPPFDQLCSAKSGILTPDWFPFLCVQSPLNNSPFLGYFFDGSTSSSRVSSVKLPLQTTPGKLSNGYEQGDPILTPRNDYFTVPQPFIAGSCARNAPVPFLRNIEAECVATCKEAERVGSDITINSGVGNNIKAEVTYEERNPALLPCPGKECRNVSFAEDYIIIWEGKRIKELKIKVLYGGMCPEETITQKFTVTFVSADATSMEEFSGNPGYQLGKPVRAANLTSLAPVTSLKLWKPVGENLCSSGNLTPLLFGFNSISGCIMEVSLADTCTQLRGDVAARLNSLVQVSHVGKRGNARKNVSDDWVEVLRLNASSSTNTSEGILRGICPDVPAHLNIQIITADVGAIEGIPQEEILGVQISFSTITWQVPCGLACPENVTSLPISAAVQFIKVPAQPPVPKTRFQINYTEYDCRRNDVCWPKLFYPVTRYYTGEPYPHTLAKGLVLVFFVLLAVVLSGPWNGIFSLWLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNT7</accession>
    <name>A0A6I9YNT7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">AP1S1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925380.1">
        <property type="nucleotide sequence ID" value="XM_014069905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNT7"/>
    <dbReference type="GeneID" id="106551757"/>
    <dbReference type="KEGG" id="tsr:106551757"/>
    <dbReference type="CTD" id="1174"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000005">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925380.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="18673" checksum="8C498E7A73CE9734" modified="2020-10-07" version="1">MMRFMLLFSRQGKLRLQKWYLATSDKDKKKVVRELMQVVLARKPKMCSFLEWRDLKVVYKRYASLYFCCAIEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLMGGEVQDTSKKSVLKAIEQADLLQEEDESPRSVLEEMGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQU0</accession>
    <name>A0A6I9YQU0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcription factor SOX-14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SOX14</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926531.1">
        <property type="nucleotide sequence ID" value="XM_014071056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQU0"/>
    <dbReference type="GeneID" id="106552712"/>
    <dbReference type="KEGG" id="tsr:106552712"/>
    <dbReference type="CTD" id="8403"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022097">
        <property type="entry name" value="SOX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF107">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12336">
        <property type="entry name" value="SOXp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="9">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="7">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013926531.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="26680" checksum="29FA015D7FB4945B" modified="2020-10-07" version="1">MSKPSDHIKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSEAEKRPYIDEAKRLRAQHMKEHPDYKYRPRRKPKNLLKKDRYVFPLPYLGDTDPLKAAGLPVAATDSLLGSPEKARAFLPPTSAPYSLLDPSQFSSSAIQKMTEVPHTLATSTLPYASTLGYQNGAFGTLSCPSQHTHTHPSPTNPGYVVPCNCTAWSTSSLQPPVAYILFPGMTKTGIDPYSSAHAAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR08</accession>
    <name>A0A6I9YR08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553070</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926973.1">
        <property type="nucleotide sequence ID" value="XM_014071498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR08"/>
    <dbReference type="GeneID" id="106553070"/>
    <dbReference type="KEGG" id="tsr:106553070"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF118">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT BETAINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="418"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013926973.1"/>
        </source>
    </evidence>
    <sequence length="611" mass="68915" checksum="59BA89033F2D1EA0" modified="2020-10-07" version="1">MGNKKSWDVELNGTVLVPETKENPRKGKDELEERGQWTNKVEYVLSVAGEIIGLGNVWRFPYLCYKNGGGAFLIPYAIFLFICGIPVFLLETALGQYTSQGGITAWRKICPIFEGIGYASQVIESYLNIYYIVILSWALFYLFNSFSPVLPWSNCNNYWNSEYCVEVLNVSDSGNNTNATSPVIEFWERRTLKIAEGLHDLGTVRWELALCLLLAWIICYFCIWKGVKSSGKVVYFTATFPYLMLIVLLIRGVTLPGALDGIIFYLNPDVSRLADPQVWMDAGTQIFFSYAICQGCLTALGSYNKYNNNCYRDTFMLCFLNSATSFVAGFAIFSVLGFMAQEQGVPISKVAESGPGLAFIAYPKAVTMMPISQLWASLFFLMLIFLGLDSQFVCVESLVTAIVDLFPEVFRKKGRRELLILAIAVICYLIGLLLVTEGGMYIFQLFDYYAASGTCLLFLAIFEVICIAWVYGADRFYDNIEDMIGYRPWPLIKYCWLFLTPGLCLATFLFSLIKYEPLKYNNVYEYPPWGIAVGWVLALSSMICVPSYIIFSFLKTKGSFKERLRQLTTPSEDLPQPKKHLMGTSGRECSDVGSQHPVKEVLGKTGKETNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRI0</accession>
    <name>A0A6I9YRI0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ecto-NOX disulfide-thiol exchanger 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106552475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926240.1">
        <property type="nucleotide sequence ID" value="XM_014070765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRI0"/>
    <dbReference type="GeneID" id="106552475"/>
    <dbReference type="KEGG" id="tsr:106552475"/>
    <dbReference type="OrthoDB" id="10039782at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007624">
        <property type="term" value="P:ultradian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038876">
        <property type="entry name" value="ENOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056611">
        <property type="entry name" value="ENOX1/2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16001">
        <property type="entry name" value="ECTO-NOX DISULFIDE-THIOL EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16001:SF6">
        <property type="entry name" value="ECTO-NOX DISULFIDE-THIOL EXCHANGER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23267">
        <property type="entry name" value="ENOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ecto-NOX disulfide-thiol exchanger 1/2" evidence="2">
        <location>
            <begin position="35"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926240.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="29501" checksum="31D34033713FCB14" modified="2020-10-07" version="1">MGIRREEEMDMSDDDSDDNPSNKKLRMEDSALAIQAYALKEENDSLRWQLDAYRNEVELLKQEKGQLFRTEESLTKDQQLQFLQQTVQGMQQQLLKIQEELNNKKLELEQAKEEHSHTQAMLKILQEQLKKNAKELETNGHDESQAKEINGLTAPLVNQDREKNAEKRSQPLKSEKEALLIGIISTFLHVHPFGANIEYLWSYMQQLDSRISANEIEMLLMRLPRMFKQEFSGVGATLEKRWKFCAFEGIKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRV9</accession>
    <name>A0A6I9YRV9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="19">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106553324</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:24802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:18322"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:24814"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013927273.1">
        <property type="nucleotide sequence ID" value="XM_014071798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRV9"/>
    <dbReference type="GeneID" id="106553324"/>
    <dbReference type="KEGG" id="tsr:106553324"/>
    <dbReference type="OrthoDB" id="1732691at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000005">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000002">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF70">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 19" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11 19" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 18" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="16">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013927273.1"/>
        </source>
    </evidence>
    <sequence length="356" mass="39623" checksum="DF0A5933A5583A4C" modified="2020-10-07" version="1">MKGCQNDHGDGCTEAAAKPTTNSFKADDLVINPSTTLKEKPDPNGLVFGTVFTDNMLFIEWSFTSGWEVPQIKPLENLSVHPAISALHYAVELFEGMKAYRGVDGKIRLFRPRLNMDRMLRSAARATLPGFDKEELLQCIQKLVEVEKEWVPYSTSASLYIRPTLIGTEPSLGVKKPSRAILYVILSPVGPYFSSGSFNPVSLWADPKYVRAWKGGTGDCKLGGNYGSSIYAQREAMELGCQQVLWLYGDDHQITEVGTMNLFLYWRNEDGEDELATPPLDGIILPGVTRQSILDLARKWGEFKVSERYITMSDLTTALKEKRVKEMFGAGTACVVCPVSTILYLGENLQIPTMEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSE5</accession>
    <name>A0A6I9YSE5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106553150</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927066.1">
        <property type="nucleotide sequence ID" value="XM_014071591.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106553150"/>
    <dbReference type="KEGG" id="tsr:106553150"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1045"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1084"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1116"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1205"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1240"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1266"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="1046"/>
            <end position="1310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927066.1"/>
        </source>
    </evidence>
    <sequence length="1314" mass="149151" checksum="52CB3C4BA29659E7" modified="2020-10-07" version="1">MEYGMILPYSSLVHEEKKKAILKVILISENDMAASSQKYSIFSFNFYGKRKAKCFLTLGQDETDPQNHVRPGELFTTGIVSATKARFLPLTFNTVPLTRISLSTSTKYWKILSYLFAVQEINQDPHLLPNLTLGYNIYEGYFNTRMTADGLLDLLSAGESNVPNYSCGREKTPLVVLEGAETDIAIQISTFLNAYKIPQISYTFASHILSDKTRFPFFYPMVPIEGVQYPVIIKLLLHFKWTLVGLVAPDTSNGQRFINMLISMLTEKGICAVISQRFTVVASELKIKLRQYYKWKQVNVFVYHAETSYFLEGIAVIEKVLTAFKKPIVGKIWITTARWDLTLGLKVSISSLPNVHAIFSFLMKTKKVANYDGFKLLYSFMDQFLEEAFQCSYGKAASSVKVWRRCREREDLEPLPREKIEQILALDSYFIYNTIWAVARALNLANLSQSRRGMRKPVKWLEIQSLKPWQLHSFLQKPQFYNNSMEGVYLDEKGDLAANLDILNSVVSANKTITRMTLGHLGRHTFAINQNSIARMEMLSKFNHDSRSLLLNITQGYNIYENYFSEQITLDALLDVLSDGEANVPNYRCGKQKNTLAVLEGANADLSILVSNMLGTYKVPQISYSFVSPILRDKIQFPFFYPMLPAEGVQYPGMVKLLLHFRWTLVGLMAPETDQGERFMRTMTSMLIKNGICPVLSQVFSIKIRNLVLSLEPFAKWNQVNVFLYGTEISSFQIGIFIMHVMLTHLKQPVSGKVWIMAACWDLTWDFIESSVSTFQNICGFFSFLVQTNQRVAFDAFKQFHSSMKRFVEKSFTCSSIKDVLSVKVWRWCREREELELLPQGEMQRILSLDSFFTYNIIWAVGLALHSAYFSKSKRPVRNSGENLGGPRLKAWQFHAFLQKPQFYNHSIDGVYFDENGDPAVDLDIVNWIVYTNKSVTRIQSGHLERQGIQGSKLVINQNKIAQMEKMNKPLPPSRCVESCHPGFRKVAQEGRPICCYDCIPCAEGTISTQEDSEKCTPCPEDQHPNISRDDCIPKIKTFLNYKDYLGIMLISIAVFLSLTTAIVLGIFIKFLDTPIIKANNRDLSYILLVSLQLSFFTSFLFIGQPRRATCLLRQITFSIIFSVAISSVLAKTITVVLVFLATKPGNKVQKWLGKSLSNFIIVSCSGLQIVICSSWLGTSPPFPESDLHSQPGEIILQCNEGSVTMFYCALGYMGILAAICFTVAFMARNLPGTFNEAKLITFSMLVFCSVWISFVPTYLSTKGKYMVAVQVTSILASSAGLLGCIFIPKCYIIILRPELNTKEQLIMKAKREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSR0</accession>
    <name>A0A6I9YSR0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DC-STAMP domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DCST1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927327.1">
        <property type="nucleotide sequence ID" value="XM_014071852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSR0"/>
    <dbReference type="GeneID" id="106553378"/>
    <dbReference type="KEGG" id="tsr:106553378"/>
    <dbReference type="CTD" id="149095"/>
    <dbReference type="OrthoDB" id="5985669at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051856">
        <property type="entry name" value="CSR-E3_Ligase_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012858">
        <property type="entry name" value="DC_STAMP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058842">
        <property type="entry name" value="DCST1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041">
        <property type="entry name" value="DENDRITIC CELL-SPECIFIC TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041:SF17">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DCST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07782">
        <property type="entry name" value="DC_STAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26037">
        <property type="entry name" value="zf-RING_DCST1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Dendritic cell-specific transmembrane protein-like" evidence="7">
        <location>
            <begin position="222"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase DCST1-like C-terminal" evidence="8">
        <location>
            <begin position="333"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927327.1"/>
        </source>
    </evidence>
    <sequence length="393" mass="45963" checksum="C22E998117B038C3" modified="2020-10-07" version="1">MGRKYQRLKGQKRKRVKSPNTSPVANVRHNLGEVVRSISCTVELQIENSKKAWKISLAPMRKILKDMVRSGKRLRSESRDISRSFTELNKQVESRAGMESRLARQAEGGDSTQEVYEAKTRMRCKRLLIINPVPHVLCARYEEHCLDTVEKKKKRSLLPLRRAEESRFIDPLRLAFQPAETKSVLLELMGCLPPLVFLLTACALDSLLYTIFSTIRSHSFIQYSFRKCLPDPRGMSWEEYVKTAIPLGALVMLCFLQVYVFRIRRVIAAFYFPKREKKRIIYLYNEMLRQRLAFVIVQRKRIILRARRRKRVRKPLLNQIGHWCPFLKRFLIRRCILCNRPESRRSLTCSNPDCETVYCRICWKDLGQTCFACNPDDLVSDDESSDGEMGYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT53</accession>
    <name>A0A6I9YT53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15 16">SLIT and NTRK-like protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17" type="primary">SLITRK3</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLITRK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927722.1">
        <property type="nucleotide sequence ID" value="XM_014072247.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013927723.1">
        <property type="nucleotide sequence ID" value="XM_014072248.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013927724.1">
        <property type="nucleotide sequence ID" value="XM_014072249.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013927725.1">
        <property type="nucleotide sequence ID" value="XM_014072250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT53"/>
    <dbReference type="GeneID" id="106553697"/>
    <dbReference type="KEGG" id="tsr:106553697"/>
    <dbReference type="CTD" id="22865"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000001">
        <property type="entry name" value="SLIT and NTRK-like family, member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773:SF6">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636658" evidence="11">
        <location>
            <begin position="29"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="649"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="12">
        <location>
            <begin position="232"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="12">
        <location>
            <begin position="561"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="327"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="619"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="621"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013927722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013927724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013927725.1"/>
        </source>
    </evidence>
    <sequence length="963" mass="107725" checksum="20207AF6D53EDBBD" modified="2020-10-07" version="1">MKPSMADPPRQRRMLWITLLSTIAVAWTTPIPLIEDSEEIEEACFDPCYCEVKESLFHIHCDNKGFINISQITESWSRPFKLYLQRNSMRRLYTNSFLHLNNAVSINLGNNALQDIQTGAFNGLKILKRLYLHENKLDVFRNDTFLGLESLEYLQADYNVIKRIESGAFRNLSKLRVLILNDNLIPMLPTNLFKSVSLTHLDLRGNRLKVLSYRGMLDHIGRSLMEIQLEENPWNCTCEIVQLKSWLERIPYTALVGDITCETPFHFHGKDLREIKRNKLCPMLSDSELVSVGVPQLPSSKENAWPTKPSSMLSSFHFTASSVEYKTSIKQPKPTKQPRAPKPPPTPRGLYPAPNQPPIAAYQTRPPIPIICPTGCSCSLHINDLGLTVNCKERGFHNISELLPRPINAKKLYLSGNLIQKIYRSDFWNFSSLDLLHLGNNRISYVQDGAFINLPNLKSLYLNGNDIERLTPGMFRGLQCLHYLYFEYNQIREIQPAAFSLMPNLKLLFLNDNLLRTLPTDAFAGTSLARLNLRNNKFLYLPVAGVLEHLNAIVQIDLKLNPWDCMCDLVPLKQWIETISSVIVVGDVLCASPENLTNRDVRSVELEVLCPEMLHVATASPAPPPSGHPSPTSPSPYELSPAGGGPVPLSVLILSLLVLFFSAVFIAAGLFAFVLRRRRKKLPFRNKQRQEALDLTGIQMQCHHLFEEAGGGGGGGMSASPEKAPPMGHVYDYIPHPVTQMCNNPIYKPREEDEVIAGESGPPGDILLGGGNVGTTCVGEQFVDPTKESGNSSCYRTLLEKEKEWNLAVSSSQLNTIVTTNHHPHPQPSGGGIGVGIPLLGELALVPPVFHHEKNGGVVLFPPTGAVLDNRERPPLVPPCSVGFVDCLYGTVPKLKELHVHPPGMQYPDLQQDARLKETLLFSAGKGFPEHHQTPQSEYLELRAKLQTKPDYLEVLEKTTYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTW1</accession>
    <name>A0A6I9YTW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Spastin</fullName>
            <ecNumber evidence="10">5.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 17" type="primary">SPAST</name>
        <name evidence="10" type="synonym">SPG4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.</text>
            <dbReference type="EC" id="5.6.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Cytoskeleton</location>
            <location evidence="10">Microtubule organizing center</location>
            <location evidence="10">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <text evidence="10">Forms an intramembrane hairpin-like structure in the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the AAA ATPase family. Spastin subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.1" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013927085.1">
        <property type="nucleotide sequence ID" value="XM_014071610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTW1"/>
    <dbReference type="GeneID" id="106553166"/>
    <dbReference type="KEGG" id="tsr:106553166"/>
    <dbReference type="CTD" id="6683"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032506">
        <property type="term" value="P:cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031117">
        <property type="term" value="P:positive regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02679">
        <property type="entry name" value="MIT_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19524">
        <property type="entry name" value="RecA-like_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000036">
        <property type="entry name" value="Spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000006">
        <property type="entry name" value="Spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03021">
        <property type="entry name" value="Spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017179">
        <property type="entry name" value="Spastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF86">
        <property type="entry name" value="SPASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="10">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="intramembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="10">
        <location>
            <begin position="80"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="MIT" evidence="15">
        <location>
            <begin position="107"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="362"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="212"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="370"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013927085.1"/>
        </source>
    </evidence>
    <sequence length="604" mass="65988" checksum="F4E79A1B6EC825C9" modified="2020-10-07" version="1">MNSPGGRGKKKGLSASSSPTRTAGASPSSAAGSASASHRQQAAVASPHKRNLYYFSYPLFAVFALLRFLAFQLGLLFAWLCERLSRGGALMAAAKGTSRAGDAPESVRTCHKRAFECISTALRIDEDEAAGQKEQAVEWYRKGIEELEKGIAILVIGQGDHYDRARRLQSKMMTNLAMAKDRLQLLEKMQAVKQIPKLQMEVRSDNTNLVCRNGHLQSENGTVPKKKDPLSHSSNSLPRSKSVAKAGSGGFSGHQRTPSYTGVSNFSAPRVPSAATQKVTPKNNRTTKPSTPSTAVRKKKDPKIFKNVDSNLANLILNEIVDSGPSVNFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNSTFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSSGEDRILVMGATNRPQELDDAVLRRFTKRVYVSLPNEETRLLLLKNLLSKQGNPLTQKELAQLARMSDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFIESLKKIKCSLSPQTLEAYIRWNKAFGDTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUA4</accession>
    <name>A0A6I9YUA4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Leucine-rich repeat and transmembrane domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LRTM1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927887.1">
        <property type="nucleotide sequence ID" value="XM_014072412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUA4"/>
    <dbReference type="GeneID" id="106553834"/>
    <dbReference type="KEGG" id="tsr:106553834"/>
    <dbReference type="CTD" id="57408"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000015">
        <property type="entry name" value="Leucine rich repeat containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="356"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="15">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013927887.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="46902" checksum="D70C3B1C67FB564E" modified="2020-10-07" version="1">MTPGLNEIQVTKRIPKTGGRCTYTGTLITRRSELSEETSVVQTNFFIDVDYKAFATILAECMKKYISKIMHKGSLFFVSSIIILVEVVTSCPLKCLCHPNSRTVDCRNQGLAKVPSHLPAETQTLLLSNNDIRKLSRRTFRSTQTLKILDLSNNSISGLPPETFIELKHLQILNLTNNFIEYVDNKMFSSLPRLRALDLSSNNISRLPRTLGNKTENITLLSLKYNHLQEIDRLLLESLPNLKMILFKGNFWQCNCQVFGLKLWMETFLYNGGISDEIICSIPENRKGKDLLKIPYELYGTCFPTASQDHQVYWHHSSRHKGSAKHTHPSEYGGNSTHINCERKSKPRPVNLRHSVVTVAITGVVCGIICLMMLAAAVYGCAYAVITAKYHQENSYLGKEKGSHEEKEPFENSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWR1</accession>
    <name>A0A6I9YWR1_9SAUR</name>
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        <submittedName>
            <fullName evidence="4">C2 calcium-dependent domain-containing protein 4C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">C2CD4C</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
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            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
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        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043549">
        <property type="entry name" value="C2C4C/C2C4D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46291:SF5">
        <property type="entry name" value="C2 CALCIUM-DEPENDENT DOMAIN-CONTAINING PROTEIN 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46291">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="319"/>
            <end position="435"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="258"/>
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            <end position="76"/>
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            <begin position="284"/>
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        </location>
    </feature>
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            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013929093.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="47479" checksum="0E4D08404CADBFFB" modified="2020-10-07" version="1">MWLLEKIRGSVDNSGSRGNDSADKQSKGPLYSNVLTPDKIPDFFIPPKLPTVPPEADEGNTKPNLGTSASEQNLSARKTPRSPRLPVKAVSESKNLLKAASRHIIQIESADECMSEEDYSTNADPQSQTAMSLPYVPKAQTSYGFATLMESPHTRRKESLFHSEHTSLCPSQSSSPSSQRRSTSGGSKTNGESAHLNPSDISMSLMNPYRYFSGGESDTCSSAESSPFGSPLLSRSVSLLKIFSQESQSKVIKLKHSVARNSSLSTDDSSADTSPSSQRRMRCATPPTGGATGGVQPQPLTPADPQDRFHKEHVLSFSKGGTIRLVVHYNPSNARLRVRVVAAEDLYDKHCDIRSINCCAALYLNPGKVQKQRSTIIKNSRNPIFNEDFFFDGLGLSNVRKMSLKVKVVNKGSSLKRDTLMGEKELPLTSLLPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXG6</accession>
    <name>A0A6I9YXG6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Citron Rho-interacting kinase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
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            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928992.1">
        <property type="nucleotide sequence ID" value="XM_014073517.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106554784"/>
    <dbReference type="KEGG" id="tsr:106554784"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928992.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="14721" checksum="C5C40D0F21A4B183" modified="2020-10-07" version="1">KKEGREGGEERERKKRKKVRKNERKRKEKRKKKERKRNRKRKEEKERELEEKHREAQLAAQHLELHLKQKEQMYEERLKVLDNQMKKDLADKDTLENMLRKHEEETHEKCKILAEQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXZ5</accession>
    <name>A0A6I9YXZ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Dynein heavy chain 5, axonemal-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106555266</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Bifurcata</taxon>
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            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
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            <taxon>Thamnophis</taxon>
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    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929543.1">
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    </dbReference>
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    <dbReference type="GeneID" id="106555266"/>
    <dbReference type="KEGG" id="tsr:106555266"/>
    <dbReference type="OrthoDB" id="424310at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF63">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="14">
        <location>
            <begin position="26"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013929543.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="35229" checksum="61ABA5557B9C0C3C" modified="2020-10-07" version="1">MNTRKIPFGNNLNLTEMLIDAPTVSEWNLQGLPNDDLSIQNGIIVTKAARYPLLIDPQTQGKIWIKNKETRNELQITSLNHKYFRNHLEDSLSLGRPLLIEDVGEELDPALDHVLEKNFIKTGSTYKVKVGDKEMDVMNGFRLYITTKLPNPAYTPEISARTSIIDFTVTMKGLEDQLLGRVILTEKQELEKERTDLIEDVTANKRKMKELEDNLLYRLTSTQGSLVEDESLIIVLSNTKRTSEEVTQKLQTSVETEVQINSAREEYRPVATRGSILYFLITEMSMVNVMYQTSLRQFLGLFDLSLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY70</accession>
    <name>A0A6I9YY70_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Midnolin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MIDN</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929252.1">
        <property type="nucleotide sequence ID" value="XM_014073777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY70"/>
    <dbReference type="GeneID" id="106555011"/>
    <dbReference type="KEGG" id="tsr:106555011"/>
    <dbReference type="CTD" id="90007"/>
    <dbReference type="OrthoDB" id="1916003at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01804">
        <property type="entry name" value="Ubl_midnolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000180">
        <property type="entry name" value="midnolin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039336">
        <property type="entry name" value="Midnolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23010">
        <property type="entry name" value="MIDNOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23010:SF1">
        <property type="entry name" value="MIDNOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="26"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="171"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013929252.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="42900" checksum="1C934D65AD8AC5EB" modified="2020-10-07" version="1">MDHQPTPRSCSRGPPACDTVQSDPPMNVFIHTTTGARYELAVPVEETVEGLKRRLSQRLKVPKERLALLHKESRLNSGKLQDLGVGEGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQVNDFLSGRSPLTLALRVGDHMMFVQLQLAAQQNSGQLQHRHVIAGRGEQAGGATAPAPAAGPAASSCRTLPGSTPASSPTFARLPTAPACPPSPVPSPAASPSPMQTMPLYCTTANPTPVTAGMFHSHGTSAQTVNGGGSGSVVSSCSEVDCTSRSKSSPSSSPSQASRTRKHGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPQSLTQLRCQTQCSLSTPSLDLNNHTTSERTAAAANQPLHLGVQCPSQAATCKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY78</accession>
    <name>A0A6I9YY78_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Platelet-derived growth factor receptor-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106554411</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
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            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Forms a complex composed of PDGFRL, TNK2 and GRB2.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928540.1">
        <property type="nucleotide sequence ID" value="XM_014073065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY78"/>
    <dbReference type="GeneID" id="106554411"/>
    <dbReference type="KEGG" id="tsr:106554411"/>
    <dbReference type="OrthoDB" id="9873386at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:catalytic activity, acting on a protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000247">
        <property type="entry name" value="Vascular endothelial growth factor receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000479">
        <property type="entry name" value="Vascular endothelial growth factor receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000548">
        <property type="entry name" value="vascular endothelial growth factor receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000949">
        <property type="entry name" value="vascular endothelial growth factor receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042495">
        <property type="entry name" value="PDGFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055229">
        <property type="entry name" value="VEGFR1-3_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055238">
        <property type="entry name" value="VEGFR1-3_N_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15360">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR RECEPTOR LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15360:SF4">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22971">
        <property type="entry name" value="Ig_VEGFR-1-like_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21339">
        <property type="entry name" value="VEGFR-1-like_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22854">
        <property type="entry name" value="VEGFR1-3_N_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01832">
        <property type="entry name" value="VEGFRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01835">
        <property type="entry name" value="VEGFRECEPTR3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="228"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="315"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="406"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928540.1"/>
        </source>
    </evidence>
    <sequence length="550" mass="63192" checksum="A5C6F9E8D426C40A" modified="2020-10-07" version="1">MTPPILSITEEEYIINAKETLNITCRGQHPLEWLWPGIQEDTVQMGKESDSMIMSISGIRTVKEEDCEGTETKPYCKALTLTGTQANDTGYYRCYYKYINAKIEGTTAVSTYVFVRDFEQPFINKPETLLIYKKENICVPCLVSVPDLNITLLLTTSVIYPDGKNVHWDNKRGMLVPTLLIKDSWFVQCETVINKKPFKSSFFIVHIAGNELYDIQLFPRRAMELLVGEKLVINCTVWAEFNSGVDFQWDYPTKQMNRKAIELPERRSQQTHTELSSILIIQNVSQEDLGKYTCKASSGEQNLKESIDVIVHEKPFIYVERKKGPVIEATVGDEIVKLPVKVVAYPQPEFQWFKDGKLISNRQSQYSLQIKDVAEQNSGSYTLVLKNGLAGLEKSINLQLVVNVPPQILEKEASSPNIYSRKNRHVLTCTVYGIPAPEKIQWQWRPWTPCRMFPQHSLPRAVQRHQRDRMPECKDWKDVSQMDAVNPIESIDTWTEFVEGRNKTISNLIIQEANVSVMYKCVASNKVGRDERLIYFYVTKYSLAIVLTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYK4</accession>
    <name>A0A6I9YYK4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Copine-6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928655.1">
        <property type="nucleotide sequence ID" value="XM_014073180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYK4"/>
    <dbReference type="GeneID" id="106554495"/>
    <dbReference type="KEGG" id="tsr:106554495"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF5">
        <property type="entry name" value="COPINE-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Copine C-terminal" evidence="2">
        <location>
            <begin position="48"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928655.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="26290" checksum="9F905C449D052512" modified="2020-10-07" version="1">MSRTARPSSVSSIPRSPFSSRMSEKSAFPMPTMMMERGRREARTTASQAFGFGARTPPDFEVSHDFAINFDPENPECEKILGVIEAYKRCLPQIELYGPTNVAPIINKVAGPAAEEEKSGMPTKYRVLLILTDGVVSDMPEACDAVVRASRLPLSIIIVGIGNADFSDMRELNGDRGMLETEEGRAVRDIVQFVPVREFKKAGPNALAKTVLAEVPKQVVEYYGSKGIPPGTQKSSSPLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYW4</accession>
    <name>A0A6I9YYW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA replication licensing factor MCM7</fullName>
            <ecNumber evidence="13">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 16" type="primary">MCM7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013929903.1">
        <property type="nucleotide sequence ID" value="XM_014074428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYW4"/>
    <dbReference type="GeneID" id="106555552"/>
    <dbReference type="KEGG" id="tsr:106555552"/>
    <dbReference type="CTD" id="4176"/>
    <dbReference type="OrthoDB" id="3207464at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17758">
        <property type="entry name" value="MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000004">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000288">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008050">
        <property type="entry name" value="MCM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF26">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24901">
        <property type="entry name" value="WHD_MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01663">
        <property type="entry name" value="MCMPROTEIN7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="225"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013929903.1"/>
        </source>
    </evidence>
    <sequence length="614" mass="68606" checksum="5CAC8AB1D03F0759" modified="2020-10-07" version="1">MEGRAREDPGQPRDPQNRFPAELLRRFELYFKAPSSAKPRVIREVKADSIGKLVTVRGIVTRVSDVKPRMVVATYSCDQCGAETYQPIQSSSFMPLLMCPSKECQTNRAGGRLYLQSRGSKFIRYQELKIQEHSDQVPVGNLPRSISVSVQGENTRLVQPGDHVSVTGVFLPVRQTGFRQLLQGLLSDTYLEAHHITCMSKSEQEELGEAELTEEELRLITEEDFYDKLAGSIAPEIYGHEDVKKALLLLLVGGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYITRLAPRSQYTTGRGSSGVGLTAAVLRDPLTGELTLEGGALVLADQGVCCIDEFDKMLESDRTAIHEVMEQQTLSIAKAGILTTLNARCAILAAANPAYGRYNPKRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPCQFQPLDMGLMRRYIATCKQKQPAVPEALADYITAAYVEMRKEARASKDTTYTSARTLLGILRLATALARLRRVDLVEKEDVNEAMRLMEMSKDSLLVDRGQPNRMQRPSELIFAAIRELVPPGGAHSVQLTEAEQHCVSKGFTPVQFQAALSEYEELNIWQVNQKKTRLTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0B8</accession>
    <name>A0A6I9Z0B8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RIT1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013930433.1">
        <property type="nucleotide sequence ID" value="XM_014074958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0B8"/>
    <dbReference type="GeneID" id="106556009"/>
    <dbReference type="KEGG" id="tsr:106556009"/>
    <dbReference type="CTD" id="6016"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04141">
        <property type="entry name" value="Rit_Rin_Ric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000343">
        <property type="entry name" value="Ras family gtpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930433.1"/>
        </source>
    </evidence>
    <sequence length="221" mass="25185" checksum="54341845A2AD6EB2" modified="2020-10-07" version="1">MESTGPRPPVGVVSGPPAGQPREYKLVMLGAGGVGKSAMTMQFISHRFPEDHDPTIEDAYKMRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDETPVVLVGNKSDLTQLRQVSKDEGSALAQEFGCPFFETSAAFRYYIDDVFHTLVREIRRKEKEIVTALEKKSKPRPSVWKTLKSPFCKKKNSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0Y6</accession>
    <name>A0A6I9Z0Y6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="24">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC106556205</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="24"/>
    <dbReference type="RefSeq" id="XP_013930668.1">
        <property type="nucleotide sequence ID" value="XM_014075193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0Y6"/>
    <dbReference type="GeneID" id="106556205"/>
    <dbReference type="KEGG" id="tsr:106556205"/>
    <dbReference type="OrthoDB" id="10005095at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043121">
        <property type="term" value="F:neurotrophin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005030">
        <property type="term" value="F:neurotrophin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990090">
        <property type="term" value="P:cellular response to nerve growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05094">
        <property type="entry name" value="PTKc_TrkC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000701">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000033">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF66">
        <property type="entry name" value="NT-3 GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 21 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 21 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 24" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17 24 27" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="34"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013930668.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="39982" checksum="B39F84A04A95EFE9" modified="2020-10-07" version="1">MTRIPVIENPQYFRQGHNCHKPDTYVQYIKRRDIILKRELGEGAFGKVFLAECYNLSPTKDKMLVAVKALKDPTLAARKDFQREAELLTNLQHDHIVKFYGVCGDGDPLIMVFEYMKHGDLNKFLRAHGPDALILVDGQPRQVKGELGLSQMLHIASQIASGMVYLASQHFVHRDLATRNCLVGVNLLVKIGDFGMSRDVYSTDYYREVTRPKGYYSRTWQQQRITTMAATTVGGHTMLPIRWMPPESIMYRKFTTESDVWSFGVILWEIFTYGKQPWFQLSNTEVIECITQGRVLERPRVCPKEMYDIMLGCWQREPQQRLNIKEIYKLLHALGKATPIYLDILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1I0</accession>
    <name>A0A6I9Z1I0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase lipid-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATPase protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ATPase subunit c</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106555766</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930149.1">
        <property type="nucleotide sequence ID" value="XM_014074674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1I0"/>
    <dbReference type="GeneID" id="106555766"/>
    <dbReference type="KEGG" id="tsr:106555766"/>
    <dbReference type="OrthoDB" id="438052at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18182">
        <property type="entry name" value="ATP-synt_Fo_c_ATP5G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.20.10:FF:000003">
        <property type="entry name" value="Atp synthase f complex subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031:SF59">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT C3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="ATP SYNTHASE LIPID-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0138">CF(0)</keyword>
    <keyword evidence="7 16" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="17">
        <location>
            <begin position="80"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013930149.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="15625" checksum="30B2FDAA8844A42D" modified="2020-10-07" version="1">LLTRVEAEINFAQVYIFRVVRNSSTVLCRPLSASVLNRPQIRTDKPCCLSGAQHEVLPLLHRQFQTSAVSRDIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMVAFLILFAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2C4</accession>
    <name>A0A6I9Z2C4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Actin-related protein T2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106555787</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930171.1">
        <property type="nucleotide sequence ID" value="XM_014074696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2C4"/>
    <dbReference type="GeneID" id="106555787"/>
    <dbReference type="KEGG" id="tsr:106555787"/>
    <dbReference type="OrthoDB" id="10053773at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13397">
        <property type="entry name" value="ASKHA_NBD_actin_Arp-T1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930171.1"/>
        </source>
    </evidence>
    <sequence length="384" mass="43052" checksum="D2AA65A2805C47E1" modified="2020-10-07" version="1">MGREGFASKARLSHSHAVIIDNGSGLCKSGISGEISPRHVMPTVLGYPRSKLPHSKAKQKECYVGFEAQHRQDSLSLAYPMEAGVITCWDEMETIWKYIYDKGLGVKAFERPLLMTESPLNPKEDRAKLTQVMFECFKVPSFYLSIQAILSLYASARFTGVVIDCGFGISHAVPVYEGYCLPHAVSKLGLGGRNVTQFLRELLLENRQYFWNFPEANTVVDDIKVKLCFVALDARHRDSKHSEELPKEYKLPDGNRIKIGDPLYLAPEILFTPSNIDHKGHGLPRLVAHSIKKCDPSIRKVLYSNVLISGGSSLFPGLDERMFKGLEGEAPQGVPIKVIAPPDRWFSAWIGASIITSLSTFKQMWVTAADYREYGPNIVHRRCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2L2</accession>
    <name>A0A6I9Z2L2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106556400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930872.1">
        <property type="nucleotide sequence ID" value="XM_014075397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2L2"/>
    <dbReference type="GeneID" id="106556400"/>
    <dbReference type="KEGG" id="tsr:106556400"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF7">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930872.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="11214" checksum="EB2687891BB17CFE" modified="2020-10-07" version="1">LESTAIHWTCRGGNVEVLKFLLNKGINRNAKDKLLSSPLHVAVRTGQYECGEHLIACEADLNAKDREGKTPMDLVLQWQNGTKEVFNNLNAYKNSPIDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z345</accession>
    <name>A0A6I9Z345_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase</fullName>
            <ecNumber evidence="6">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">PLD2</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013931058.1">
        <property type="nucleotide sequence ID" value="XM_014075583.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931065.1">
        <property type="nucleotide sequence ID" value="XM_014075590.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931072.1">
        <property type="nucleotide sequence ID" value="XM_014075597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z345"/>
    <dbReference type="GeneID" id="106556594"/>
    <dbReference type="KEGG" id="tsr:106556594"/>
    <dbReference type="CTD" id="5338"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000027">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000005">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000022">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF121">
        <property type="entry name" value="PHOSPHOLIPASE D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="8">
        <location>
            <begin position="47"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="734"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013931058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013931072.1"/>
        </source>
    </evidence>
    <sequence length="917" mass="103232" checksum="7FD351E94703F7FF" modified="2020-10-07" version="1">MQPGEPDETDALQPPVAAGQPCSFLMAVYEPRPLHSQTPLFLQEVPIVARVESTERYTCGSKVRPSTVYALQLSHGRFTWSMKKLFRHFQELHRDLMKHRLLLSLLPLPRFVLQGSWPVTSSLELPPLPHGGSEVSRRPSSKQKQLEIYLNTLLEMSAYRDYHAMAEFLDVSRLSFLPELGPKGLEGMILKRSGGHRIQGLNCTGHHQVCYRWSKRWLVVKDAFLLYLKPESGVISCVLLFDPAFRVQVGKKPTETKYGVRVDNSCRSLVLKCSSYRQACWWGQQITELAAGKGQQHLQRHRHEGFAPVRQETPARWFLNGAGYFAAVADALVAAREEIFITDWWLSPEVYMKRPAQSDEWRLDLILKHKAEQGVRVCVLLFKEVGLALGLNSGYSKRALMLLHPNIKVMRHPDHVSSIVFLWAHHEKLVVVDQAVAFLGGLDLAYGRWDTPEYRLTDLEGENSRGAKGGAAPAGQEAPVDLATNQLLWLGKDYGNLIAKDWVQLDKPFEDLIDRFQTPRMPWRDVGVAVHGVAARDVARHFIQRWNYTKTIKAKYKGSGYPYLLPKSPHVPPELPFLVPGAQAADVQVLRSVDRWSAGLHECSIYKAYLEVIRSSRHYLYIENQFFISCADGRSVLNTVGDAVVQRILLAHSEKKPFRVYVLLPLLPGFEGDIAQGGSNSIQAILHFTYRTLCRGESSIVSRLQAVMGDGWRDYITFCGLRTHGELQGALLTELVYVHSKMLIADDQRVIIGSANINDRSLLGKRDSELAVLVEDRELVPSVMGGQEYAAGKFALSLRLECFRRLLGVVPEDDDCIRDPISDSFFHDVWSARVSANTSIYEEVFRCLPSDAVRSLRALREHVAVGSLASLSPGRARERLGAVRGLLVGFPLEFLAEENLLPPLNCKEGMIPVEVWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3D2</accession>
    <name>A0A6I9Z3D2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Headcase protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">HECA</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931598.1">
        <property type="nucleotide sequence ID" value="XM_014076123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3D2"/>
    <dbReference type="GeneID" id="106557023"/>
    <dbReference type="KEGG" id="tsr:106557023"/>
    <dbReference type="CTD" id="51696"/>
    <dbReference type="OrthoDB" id="10012848at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026066">
        <property type="entry name" value="Headcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031947">
        <property type="entry name" value="Headcase_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054537">
        <property type="entry name" value="HECA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13425">
        <property type="entry name" value="HEADCASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13425:SF3">
        <property type="entry name" value="HEADCASE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16002">
        <property type="entry name" value="Headcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15353">
        <property type="entry name" value="HECA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Headcase N-terminal" evidence="2">
        <location>
            <begin position="80"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Headcase middle" evidence="3">
        <location>
            <begin position="327"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931598.1"/>
        </source>
    </evidence>
    <sequence length="529" mass="58532" checksum="DC24ADF7D071EAE4" modified="2020-10-07" version="1">MPNQKSNKGKKNKRANSSGDEQENGALAATGAVGAGGVLAAVAAVGASGPMVAAGGGGSSGGATAATGSASGDSKNEAPCATPLLCSFGRPVDLEKDDYQKVVCNNEHCPYSTWMHLQCFYEWESSILAQFNCIGRARSWNEKQCRQNMWTKKGYDLAFRFCSCRCGQGHLKKDTDWYQVKRMQDEKKKKSGSEKSSGRSTSESSEDLKKCRSANKPQKGLNHDLQRRHSMDRQNSQEKGTSSSSYAVRSPCGSPGQSPPAGYSVLAPTHFSGPRSSRYLGEFLKNAIHLEPHRKNMANSGMFRNAHFDYGTSGLQAHRSGHFDAPVQFLRRLDLSELLAHIPRHKLNTFHVRMEDDAQVGQGEDLRKFILAALSASHRNVVNCALCHRALPVFEQFPLVDGTLFLSPSRHDEIEYDVPCHLQGRLMHLYAVCVDCLEGVHKIICIKCKSRWDGSWHQLGTMYTYDILAASPCCQARLNCKHCGKPVIDVRIGMQYFSEYSNVQQCPHCGNLDYHFVKPFSSFKVLEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3H4</accession>
    <name>A0A6I9Z3H4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BTB/POZ domain-containing protein KCTD1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">KCTD1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930521.1">
        <property type="nucleotide sequence ID" value="XM_014075046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3H4"/>
    <dbReference type="GeneID" id="106556082"/>
    <dbReference type="KEGG" id="tsr:106556082"/>
    <dbReference type="CTD" id="284252"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18387">
        <property type="entry name" value="BTB_POZ_KCTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000003">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048599">
        <property type="entry name" value="BTB_POZ_KCTD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048595">
        <property type="entry name" value="KCTD1-15-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052787">
        <property type="entry name" value="MAVS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021893">
        <property type="entry name" value="ZMYM2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21446">
        <property type="entry name" value="DUF3504 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21446:SF12">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12012">
        <property type="entry name" value="DUF3504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20871">
        <property type="entry name" value="KCTD1-15_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="723"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930521.1"/>
        </source>
    </evidence>
    <sequence length="950" mass="104238" checksum="A34AD0FB06E9512C" modified="2020-10-07" version="1">MLVAAGFLRLTSASKETSKLTSFVASPVYSQASVASRSAATAAAREATEKPPGICSTLPFGCCLRTNQPLSCVTRRLRLRLQPVASGCSSKTARCLRRELEEERDRRACAGEDEDGEDEDEIQEVQITGDEEGADGGLLLLDDDEEEEEDDVMMGLAWDPEEEPGHMIRMDQSSRALLDKAAAAVCGVRGSLGGVRRGGTGAIGAAAGGAPSVPVSARGMTTSVVSKAVAPLAAEDSDPEAELLLQRPERARLSENTRLATRYAVRIFREYLSEKAQSPDFETMDKGALCRVLRSFYAEARSKSGQLYSKSSLISIRSSLNRYLNEPPYCRTLDLTKDPELRAANLTLAAVIRKLEEQGAGPVVQKQAITRADLRKLYTCNVFSTQSPFGLLNKVWFETCMYFCTRGRENQRELEEDSFGLAMDEDGRKFVYFKALGPYHKSRSSSWSKKRAESSDEENLPRMYETGTEFCPYASFVKYLAKRNPLCKAFFQRPRDHCSEGDVTWYENKAIGKNLLGTRMQMLSKAAKLSKTYTNHCIGAVSIATLNSIAGIGTKLTSGTTGGGGFYGLNGRGRLPPIPANNTYILPKDGDNHPSVKAEAAAVLVPTKRSHEAMYAAGGGAAVVGDACGPSSSPKRLCLRPAEPLDTAAVVVVSVKHDPLPLLIPEANGPRSTNSPTVISPAMVSPTQDSRPNMSRPLITRSPASPLNNQGIPTPAQLTKSNAPVHIDVGGHMYTSSLATLTKYPESRIGRLFDGTEPIVLDSLKQHYFIDRDGQMFRYILNFLRTSKLLIPDDFKDYSLLYEEAKYFQLQPMLGEMERWKQDRETGRFSKSCECLVVRVAPDLGERITLSGDKSLIEEVFPEIGDVMCNSVNAGWNHDSTHVIRFPLNGYCHLNSVQVLERLQQRGFKIVGSCGGGVDSSQFSEYVLKRELRRPSRSSSVIRIKQEPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3U5</accession>
    <name>A0A6I9Z3U5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch-like protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106557142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931768.1">
        <property type="nucleotide sequence ID" value="XM_014076293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3U5"/>
    <dbReference type="GeneID" id="106557142"/>
    <dbReference type="KEGG" id="tsr:106557142"/>
    <dbReference type="OrthoDB" id="6482909at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF469">
        <property type="entry name" value="SI:DKEY-260J18.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="73"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931768.1"/>
        </source>
    </evidence>
    <sequence length="643" mass="72279" checksum="A965FB3AE67EBBF8" modified="2020-10-07" version="1">MTSNEASHSVYSENVDLFSASQNGSLYTLKLWIMDAKKDFNNSQEIDIRVKPTTLGYYVTQGLMQLHKTQQLCDAILVAEGKHFPCHRALLASFSPYFRAMFTRPFRECWERQVVFEDMASSILQIILDYLYTGRLLLIPEIAQDLFTAANRLVILPLQDIIGRFLADSISMDSCLEIYALAYAHNHHTLLRVASRHITLNFEPLSEHESFPGLDLSTVISIVSSNKLIVSSELTVYQAVQNWVRSQPAEHFSLIKKLMQQVRLPLLTSEEITRVQKDIIAEYGHAHLQWEQLDGVGRLLKSGGLRHGMYDDWIVSLGLFVSNMQGGVVERLKTHFLGFNLQTESWEEIPPLMYLDSSGFLSVGHKLYVSGGQRLNGSVLCNLHEFDALTGRWMQLPSMSVPRREHGFLACQQKLYALGGRSCRTVLDSAESFDLEKKSWSPIANLPFAVSHFASASLNKKLYLIGGFSHTRANGLAHRGILIYNTSVNVWNQMPLAFQCYKSTAVAMDNGIFVIGGLVEESSRRETPGTVIRALISGTHKCFFLSKDGTVSQDVAIPDLPILVLPPCAVQWRRRIHVLVGNTIYQWKPGEAHWTRSRKSAPNTRVTFLTVVNGVTLKVPKKTLQPLLREASAALTALWVTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3Z5</accession>
    <name>A0A6I9Z3Z5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">POLR2H</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4 6">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930585.1">
        <property type="nucleotide sequence ID" value="XM_014075110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3Z5"/>
    <dbReference type="GeneID" id="106556135"/>
    <dbReference type="KEGG" id="tsr:106556135"/>
    <dbReference type="CTD" id="5437"/>
    <dbReference type="OrthoDB" id="10249565at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000073">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930585.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="17129" checksum="558439EAA83D9E14" modified="2020-10-07" version="1">MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNVQIYPVDLGDKFRLVIASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4S6</accession>
    <name>A0A6I9Z4S6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Visual pigment-like receptor peropsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106557399</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013932108.1">
        <property type="nucleotide sequence ID" value="XM_014076633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4S6"/>
    <dbReference type="GeneID" id="106557399"/>
    <dbReference type="KEGG" id="tsr:106557399"/>
    <dbReference type="OrthoDB" id="8863409at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15074">
        <property type="entry name" value="7tmA_Opsin5_neuropsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000390">
        <property type="entry name" value="Novopsin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0157">Chromophore</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="47"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="346"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013932108.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="44945" checksum="EB4E5C28749DF691" modified="2020-10-07" version="1">MSFQISGQAPWRNNNLTFLNKDHPISEQGETIIGFYLLALGWMSWIGNSIVIFVLYKQRAVLQPTDYLTFNLAVSDGSIAIFGYSRGIIDIFNVFRDDGFLVTSIWTCQIDGFLTLLFGLASINTLTVISVTRYIKGCHPNRAYCISLSSVSFAIFLIWTTAFFWSLAPLLGWGSYTDRMYGTCEIDWTKANSSTTYKSYIVSVFICCFFVPVIVMVFSYTSIINTVKSSHALSGMGDPTDRQRRMEQSVTRISLVVCTCFIAAWSPYAVISMWSACGYTVPNLTSIFASLFAKSASFYNPIIYFGMSSKFRKDISIFFHFAKEIKDPVKLKQFKIIKQKMDNSASRGEEKKAINAQPTLRFDSEKGSHSNTPPPVNKEEGLVAFDDLPSNPDFECDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4T5</accession>
    <name>A0A6I9Z4T5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Single-strand selective monofunctional uracil DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SMUG1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. SMUG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930986.1">
        <property type="nucleotide sequence ID" value="XM_014075511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4T5"/>
    <dbReference type="GeneID" id="106556521"/>
    <dbReference type="KEGG" id="tsr:106556521"/>
    <dbReference type="CTD" id="23583"/>
    <dbReference type="OrthoDB" id="408702at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017065">
        <property type="term" value="F:single-strand selective uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19374">
        <property type="entry name" value="UDG-F3_SMUG1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000005">
        <property type="entry name" value="Single-strand selective monofunctional uracil DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039134">
        <property type="entry name" value="SMUG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13235">
        <property type="entry name" value="SINGLE-STRAND SELECTIVE MONOFUNCTIONAL URACIL DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13235:SF2">
        <property type="entry name" value="SINGLE-STRAND SELECTIVE MONOFUNCTIONAL URACIL DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="8">
        <location>
            <begin position="61"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013930986.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29354" checksum="962227DBC0874A6D" modified="2020-10-07" version="1">MNSWEVPMNFLEEGGLAERFLHIEREQIVRLNSLSFLDPVQYVYNPLDYAWEPHQDYVRKYCHSRKEVLFLGMNPGPFGMAQTGVPFGAVQLVRDWLRITGQVFRPACEHPKRPIRGLECPHTEVSGARFWGFFRSVCTEPEKFFQRCFVHNHCPLLFVSQSGRNLTPADLPAAQREQLLQICDDALCEAVKLLGFTTVIGVGRFAEQRAHKALSAAGIPVRVVGVMHPSPRNPKANKGWDAIIRAKLEELGLMALITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4T7</accession>
    <name>A0A6I9Z4T7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Probable E3 SUMO-protein ligase RNF212 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106557404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013932113.1">
        <property type="nucleotide sequence ID" value="XM_014076638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4T7"/>
    <dbReference type="GeneID" id="106557404"/>
    <dbReference type="KEGG" id="tsr:106557404"/>
    <dbReference type="OrthoDB" id="2535391at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16746">
        <property type="entry name" value="RING-HC_RNF212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042123">
        <property type="entry name" value="Zip3/RNF212-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22663:SF21">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RNF212-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22663">
        <property type="entry name" value="RING FINGER PROTEIN NARYA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0469">Meiosis</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="7"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013932113.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="29579" checksum="9C146121BD06AD04" modified="2020-10-07" version="1">MPCLIFCNVCFQKPRGAALNFNLTNCGHVICGQCLQKGKKGDCMICRVPCRTILLSKKVNSDIQSLFMGLDGLCRKYTKEITQIAQFQEKHRRHLLTHYKEKIAKLETYLKRASQQIQHIQQLQHRNSSKEVEVPPSVRKTETVAGPTRISLINPPQNGYMGSVTCRSSQLSGMTFQRNPAGSIRSSPLKMLNSGNSYISPSPFVSSQNSRNHIPNTFGQKSTQHYQSTSASSLSSGIKHPITLVNLLQRQHLGSTNLPGRSLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5V9</accession>
    <name>A0A6I9Z5V9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Dynein heavy chain 2, axonemal-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC106557356</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932052.1">
        <property type="nucleotide sequence ID" value="XM_014076577.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106557356"/>
    <dbReference type="KEGG" id="tsr:106557356"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000153">
        <property type="entry name" value="Dynein axonemal heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000008">
        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000007">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000008">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000005">
        <property type="entry name" value="Dynein, axonemal, heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF68">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="19">
        <location>
            <begin position="125"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="20">
        <location>
            <begin position="276"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="17">
        <location>
            <begin position="564"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="17">
        <location>
            <begin position="717"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="16">
        <location>
            <begin position="765"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="18">
        <location>
            <begin position="1031"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="15">
        <location>
            <begin position="1465"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="21">
        <location>
            <begin position="1610"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="22">
        <location>
            <begin position="1756"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="618"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="886"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1200"/>
            <end position="1227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013932052.1"/>
        </source>
    </evidence>
    <sequence length="2059" mass="235930" checksum="D2B38DA10DAC723F" modified="2020-10-07" version="1">MVFLDEEQVEHLLDVGHEGYGFLLEADEYTEQTVGEVKSASSLLVWNTVAPKTGLIDEVVKCSWHGFKWDALPLLRVEVVKTFTWVLGTYHTLFAGLARTKPPNAELLAYWYLEFVKFKHLCFPHMGCXTEYNGIVALCKLFTSLAEEGLNLTDTENYSTLVEMYFVFSMIWSLCAAVDEEGRKKIDNFLREIEGTFPNKDTVYEYYVDPKRKQWVAFEDNLPKMWRYPPNIERERAGGPNEPDSQIRRIFGTMINQKLQNFEEAVKPIGNVITEATVELYNSVVQRFLPTPAKIHYLFNLRDISKVFQGMLRAHKDFHDTKASLTRLWIHECFRVFSDRLVDIPDMETFVALLGEKLGTFFDVTFHHLCPNKRSPIFGDFMRNVYEDMTDMGVLKAEMERALGDYNRTPGVVPMRLVLFRDAIEHSEWGSLTTSNNVQSIIESRVEKRTKGVYVPMGGKSMITFMDDLNMPAKDTFGSQPPLELLRLWLDYGFWYDRTKQTIKHIKIQNHILDAARSEGIQESQDSLFAYLIERVRNNLHVVLCLSPVGDPFRMERLPREGLQESKIATTGMQKRVAKIFVTMHWSVARYSHKMLLELRRHNYVTPTNYLELVSGYKKLLAEKRKELSDQANKLRNGLFKIDETREKVEVMTLELEEARRKVAEFQKQCEEYLVIIVQQKREADEQQKVSVRGSEPPISRPNLLWAASRDGSRFGENRIRQYPALVNCTTIDWFSEWPKEALLEVAEKYLEGVDLGTVEKGSFDTALESLNKKDLTEIKSYGRPPTLVETVMQAVMILRGNEPTWTEAKKQLGEPNFIKQLVYFDKDNISDKVLKKISAYCSQPDFQPDIIGRVSVAAKSLCMWVRAMEMYGRIYRVVEPKRARMNAALAQLAEKQAAQAEAQERLREVAEKLEMLKKEYEEKLAQKEELRKKSEEMEMKLDRADKLVSGLAGEKIRWEETVKGLEEDLGYLVGDCLLASAFVSYMGPFLSVYRDEMLSHIWMKQIRQFHVPCSPMFTCDSFLSNPTMVRTWNIQGLPSDDFSTENGIIVTRGNRWPLMIDPQCQASKWIKNMEAKKGLKIIDLQMPDYLRILENAIQMGSPVLLQNVQEELDPTLAPILNKSVTKVGGRMLIRLGDKEVDYHPDFRFYLTTKLSNPHYTPETSSQTTIVNFAVKEQGLEAQLLGIVVRKERPELEEQKDSLVLNIAAGKGKLKELEDEILRLLNEATGSLLDDVQLVNTLQTSKVTATEVTEQLETSEMTEIKIDTAXXAYRPCAQRASILFFVLNDMGRIDPMYQFSLDAYIDLFNLSINKSHRSHKLEERIRHLNEYHTYAVYRYTCRGLFERHKLLFSFQMCAKILEMSGSLKMDEYNFFLRGGVVLDREGQMDNPCTSWLSDIYWDNVTELDKLTNFHGIMNSFEQYPRDWNLWYTNSKPEKAMLPGANSHLILSSPAFCLQVVDDSTTKTPLIFVLSSGVDPTSSLLQLAEQSGMAQRFHALSLGQGQAPIATRMIKDGVHQGNWVFLANCHLSLSWMPQLDKLVEQLQVEEPHPSFRLWLSSSPHPEFPISILQAGIKMTTEPPTGLKANMKRLYQLITDPQFNRCTKPSKYKKLLFALCFFHSVLLERKKFLQLGWNIIYGFNDSDFEVSENLLSLYLDEYEDTPWDALKYLIAGVNYGGHVTDDWDRRLLTTYINDYFCDQTVAAPFYKLTVLDTYYIPKDGNLSSYKEYISLLPGMDPPEAFGQHPNADVASQITEARTLFETLLSLQPQTTPTGVAGQSREDKVLELSSDVRSKIPEEIDYEGTCKVLSTDPSPLNVVLLQEIQRYNSLLQIISSSLVELEKGIQGLVVMSTDLEEIFNCIFDARVPPMWERAYSSQKPLAAWTRDLGLRVEQFARWAKTAHPPVLFWLSGFTFPTGFLTAVLQAAARQNNISVDTLSWDFIVSTVDDNNLVYPPKEGVWIRGLFLEGAGWDKKNSCLVEAEPMQLVCPIPTIHFKPVENKKKAGKGIYSCPCYYYPNRAGTSGRPSFVIGVDLRSGAMPPDHWIKRGTALLMSLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYU2</accession>
    <name>A0A6I9WYU2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Interferon regulatory factor 2-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">IRF2BP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IRF2BP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908124.1">
        <property type="nucleotide sequence ID" value="XM_014052649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYU2"/>
    <dbReference type="GeneID" id="106538214"/>
    <dbReference type="KEGG" id="tsr:106538214"/>
    <dbReference type="CTD" id="26145"/>
    <dbReference type="OrthoDB" id="10065080at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1580:FF:000001">
        <property type="entry name" value="interferon regulatory factor 2-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1580">
        <property type="entry name" value="Interferon regulatory factor 2-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044882">
        <property type="entry name" value="I2BP1/2_C3HC4-RING_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058682">
        <property type="entry name" value="IRF-2BP1/2-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057414">
        <property type="entry name" value="Zf-C3HC4_IRF-2BP1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF17">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 2-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25457">
        <property type="entry name" value="IRF-2BP1_2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25454">
        <property type="entry name" value="zf-C3HC4_IRF-2BP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRF-2BP1/2-like middle" evidence="6">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon regulatory factor 2-binding protein 1/2-like C3HC4 zinc finger" evidence="5">
        <location>
            <begin position="325"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="140"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="240"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013908124.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="43200" checksum="1E84BB6CC7E67D33" modified="2020-10-07" version="1">MRARAEEWHGKPKAVREQLLALCGCAPFTVRFKKDHSLTGRIFAFDAAARPGYEYELKLFAEYPCGSGNVFAGVLAVAKQMFQDCLKEPGKVLSSGYKYLEYEKRHGSGDWRLLAELFTEAVRYFREPPAPDALPQQFLDGSSATGPPNVLLRAAPPARPGLRRRKASPEPEGELAAGKLTGEEQLQQRQHWLPPGSSTGLYPPPGLAHLPAQAGLPEGSLRNDPSPITALKNVADTLGGKELNPVHSTTARQNSTSPASPAASGPHRLVPRNGEPGQAATTTTSSSSSSSSSSSSSGAHSSGAAEPSGTQSAPDSSGGAGSAPLCCTICHERLEDTHFVQCPSVPGHKFCFPCSRDFIKAQGAAGEVYCPSGEKCPLVGSNVPWAFMQGEIATILAGDVKVKKERDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2V7</accession>
    <name>A0A6I9X2V7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Anthrax toxin receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106538249</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908172.1">
        <property type="nucleotide sequence ID" value="XM_014052697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2V7"/>
    <dbReference type="GeneID" id="106538249"/>
    <dbReference type="KEGG" id="tsr:106538249"/>
    <dbReference type="OrthoDB" id="10035766at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008400">
        <property type="entry name" value="Anthrax_toxin_rcpt_extracel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059:SF11">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05587">
        <property type="entry name" value="Anth_Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Anthrax toxin receptor extracellular" evidence="8">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Anthrax toxin receptor extracellular" evidence="8">
        <location>
            <begin position="91"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013908172.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="23539" checksum="BC75475652DEE4DA" modified="2020-10-07" version="1">MLIQQKAGSLSFKALEVILACSPDRFRFKPSCTESFQVVVTGNGFRHARNVDRVLCSFRINETVTLKSPWITTIRLVTIESYHSKFGNKKKKHIQYEKPFVVEDTYLLCPAPVLQEAGMEAALQVSMNDGLSFISSSVIISSTHCSDGTILAIILLILFILLALALLWWFWPLCCTVVSPWNPFSLWLVFCVKRGENHSQRKPQGCLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X358</accession>
    <name>A0A6I9X358_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Receptor-type tyrosine-protein phosphatase eta-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106538701</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908751.1">
        <property type="nucleotide sequence ID" value="XM_014053276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X358"/>
    <dbReference type="GeneID" id="106538701"/>
    <dbReference type="KEGG" id="tsr:106538701"/>
    <dbReference type="OrthoDB" id="8609993at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000185">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF10">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE, RECEPTOR TYPE, H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="136"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013908751.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="29210" checksum="1CFA78DF7FFA71E6" modified="2020-10-07" version="1">MFLCIQYVRINWYKYHSPPKGYKKEKEFIAAQGPTPTTLHDFWRMIWEQRITTIVMLTNCVENGRVKCEHYWPLDYTPCTYDDITVSVITETILSDWTIRDFCIKRIITSEVRHARHYHYTSWPDHGVPQTTDTILHFRDLVREDLDKIKDNGPVLVHCSAGVGRTGTFISLDSLLCQAEDQGEIGVFSFVEELRRNRPLMIQNEDQYVFLHQCLLDGIQSAPKNDRVILEHPAVYENILAMQDCEVSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3Q7</accession>
    <name>A0A6I9X3Q7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Abhydrolase domain-containing protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ABHD8</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909924.1">
        <property type="nucleotide sequence ID" value="XM_014054449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3Q7"/>
    <dbReference type="GeneID" id="106539624"/>
    <dbReference type="KEGG" id="tsr:106539624"/>
    <dbReference type="CTD" id="79575"/>
    <dbReference type="OrthoDB" id="9033117at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF83">
        <property type="entry name" value="PROTEIN ABHD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013909924.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="23929" checksum="2B9087F9CACE332B" modified="2020-10-07" version="1">MPIGPLARTDKRPVLKMAAGGQARLVIGRGALSVGRRVSFCTFLAHEYPELVHKVIMVNGGGPTALEPSLCSIFNMPPCVLRCLSPCLAWSFLKAGFARQGAKEKQLLKEGNAFNVSSFVLRAMMSGQYWPEGDEVYHAELTVPVLLVHGMHDKFVPVEEDQRMAEILLLAFLKVIDEGSHMVMLECPETVNTLLHEFLLWEPEPLPPEEGPPAGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4H2</accession>
    <name>A0A6I9X4H2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bactericidal permeability-increasing protein</fullName>
            <shortName evidence="4">BPI</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BPIFC</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The N- and C-terminal barrels adopt an identical fold despite having only 13% of conserved residues.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N- and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909196.1">
        <property type="nucleotide sequence ID" value="XM_014053721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4H2"/>
    <dbReference type="GeneID" id="106539050"/>
    <dbReference type="KEGG" id="tsr:106539050"/>
    <dbReference type="CTD" id="254240"/>
    <dbReference type="OrthoDB" id="9938407at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.10:FF:000001">
        <property type="entry name" value="phospholipid transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032942">
        <property type="entry name" value="BPI/LBP/Plunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001124">
        <property type="entry name" value="Lipid-bd_serum_glycop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504">
        <property type="entry name" value="BACTERICIDAL PERMEABILITY-INCREASING BPI PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504:SF17">
        <property type="entry name" value="BPI FOLD-CONTAINING FAMILY C PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02886">
        <property type="entry name" value="LBP_BPI_CETP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00329">
        <property type="entry name" value="BPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026945672" description="Bactericidal permeability-increasing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="6">
        <location>
            <begin position="26"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein C-terminal" evidence="7">
        <location>
            <begin position="209"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013909196.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="46569" checksum="584179B9DB46D198" modified="2020-10-07" version="1">MRFWWVFILLSLFLFQLEANPEVRIRITQRGLNYAKEIGMKLLEQELKARIFPDEIGTEKYFFGTVDYTVSRIQIMSISVYNSSVILIPENGIRLLLKNISVTVTTHWTFNNWLIDYQGEVEISILGLSITTDMIVALNNIGDLLVSIQNCQVSTGKIEVQLNSEGRWFYNAFVQHLEKVIHTRLESQLCLNVILRIQKETEVLKGLKDIKDSGSGAVFISGVNITAILSSYFNLTTDALESIIPTITHIYAKPLPVIMNIRIIDAPVVSLHDNKCILEFSASMEVLAKMPNSTTESMFTLNITTRTHADLFIFNRKLLTTLCLSSFHISLVHSNVGLFEASLLDNFLSYILRTGIIPAANFKLKDEFPLSVLDPVILMKPSIRMYEGFLLISSDIQSLRKRGTSRSPSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5A9</accession>
    <name>A0A6I9X5A9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="45">5'-AMP-activated protein kinase catalytic subunit alpha-1</fullName>
            <ecNumber evidence="7">2.7.11.1</ecNumber>
            <ecNumber evidence="6">2.7.11.26</ecNumber>
            <ecNumber evidence="5">2.7.11.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="37">Acetyl-CoA carboxylase kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="38">Hydroxymethylglutaryl-CoA reductase kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="46">Tau-protein kinase PRKAA1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="51" type="primary">PRKAA1</name>
    </gene>
    <organism evidence="50 51">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="51" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>L-seryl-[3-hydroxy-3-methylglutaryl-coenzyme A reductase] + ATP = O-phospho-L-seryl-[3-hydroxy-3-methylglutaryl-coenzyme A reductase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23172"/>
            <dbReference type="Rhea" id="RHEA-COMP:13692"/>
            <dbReference type="Rhea" id="RHEA-COMP:13693"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>L-seryl-[acetyl-CoA carboxylase] + ATP = O-phospho-L-seryl-[acetyl-CoA carboxylase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20333"/>
            <dbReference type="Rhea" id="RHEA-COMP:13722"/>
            <dbReference type="Rhea" id="RHEA-COMP:13723"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="36">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="7"/>
    <dbReference type="EC" id="2.7.11.26" evidence="6"/>
    <dbReference type="EC" id="2.7.11.31" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013910584.1">
        <property type="nucleotide sequence ID" value="XM_014055109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5A9"/>
    <dbReference type="GeneID" id="106540101"/>
    <dbReference type="KEGG" id="tsr:106540101"/>
    <dbReference type="CTD" id="5562"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0047322">
        <property type="term" value="F:[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0004679">
        <property type="term" value="F:AMP-activated protein kinase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12199">
        <property type="entry name" value="AMPKA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14403">
        <property type="entry name" value="UBA_AID_AAPK1"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000079">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000014">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000136">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039137">
        <property type="entry name" value="AMPKA1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049020">
        <property type="entry name" value="PRKAA1/2_AID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028797">
        <property type="entry name" value="PRKAA1_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF87">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21147">
        <property type="entry name" value="AMPK_alpha_AID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20 47" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="25" id="KW-0072">Autophagy</keyword>
    <keyword evidence="29" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="18" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="23" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="30" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="33" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 51" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="28" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 47" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="35" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="50" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="24" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="34" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="26" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="32" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="31" id="KW-0804">Transcription</keyword>
    <keyword evidence="27" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="22" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="49">
        <location>
            <begin position="19"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="48">
        <location>
            <begin position="472"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="48">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="48">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="binding site" evidence="47">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00032270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00032865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00047775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00048291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00048417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00049758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00049783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="48">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="49">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="50">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="51">
        <source>
            <dbReference type="RefSeq" id="XP_013910584.1"/>
        </source>
    </evidence>
    <sequence length="550" mass="62674" checksum="727D71B1A3C5C04F" modified="2020-10-07" version="1">MAAADKQKHEHGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGKLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNSAVISLLKHMLQVDPMKRATVKDIREHEWFKQDLPKYLFPEDPSYSSTLIDDEALREVCDKFECTEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHFSRPHPERVPFLVAEVPRSRHTLDELNPQKSKHQGVRRAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNAVTGTYSKMSLQLYQVDSRTYLLDFRSIDDEITEGKSGTTTPQRSGSLTSYRSGQKDSDPDIQAKPSDASLTSSVTSSLDSSTSESTPRSGSHTIEFFEMCANLIKILAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X663</accession>
    <name>A0A6I9X663_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">COUP transcription factor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NR2F1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909317.1">
        <property type="nucleotide sequence ID" value="XM_014053842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X663"/>
    <dbReference type="GeneID" id="106539139"/>
    <dbReference type="KEGG" id="tsr:106539139"/>
    <dbReference type="CTD" id="7025"/>
    <dbReference type="OrthoDB" id="5873264at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06958">
        <property type="entry name" value="NR_DBD_COUP_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06948">
        <property type="entry name" value="NR_LBD_COUP-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000003">
        <property type="entry name" value="Coup transcription factor 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000016">
        <property type="entry name" value="Nuclear receptor subfamily 2 group F member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="73"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="174"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013909317.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="45724" checksum="65ABF967A01319A2" modified="2020-10-07" version="1">MAMVVSSWRDAQEDVAGGGPTGAAQPGRDPQQGASAAPHTPQTPSQPGAPSTPGDKGQQQQSSGQSQQQQQQHIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYTCRANRNCPIDQHHRNQCQYCRLKKCLKVGMRREAVQRGRMPPSQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPDLQITDQVALLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGSSFNWPYMSIQCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7L5</accession>
    <name>A0A6I9X7L5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Poly(A)-specific ribonuclease PARN-like domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PNLDC1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907368.1">
        <property type="nucleotide sequence ID" value="XM_014051893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7L5"/>
    <dbReference type="GeneID" id="106537701"/>
    <dbReference type="KEGG" id="tsr:106537701"/>
    <dbReference type="CTD" id="154197"/>
    <dbReference type="OrthoDB" id="414075at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990431">
        <property type="term" value="P:priRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990432">
        <property type="term" value="P:siRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000061">
        <property type="entry name" value="PARN like, ribonuclease domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051181">
        <property type="entry name" value="CAF1_poly(A)_ribonucleases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092">
        <property type="entry name" value="POLY A -SPECIFIC RIBONUCLEASE/TARGET OF EGR1, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092:SF22">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE PNLDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907368.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="58679" checksum="9499722163D36A98" modified="2020-10-07" version="1">MDVGAAQFPEMLPRLEELVLGCDFVGLDLEFTGLHSDSQPSLFDLPDEWYRKGRLSVQRFTVNQLGLSIFYKGVSNEYIAHSYNFFLFPATFGQMDSEFSCQTSSIQFLSHYDFDYNKFLKDGIPYMNETQEKKLQHLLSGNWMVQSFDKDKLKEVIDQVTCWIFSAEEEDSMVLHDIYGFQVIELQLILRKAFPDIWTIPLEDEKLMVKKVNPQYRWVLENTAFDPCQREQILYSARGFTNIFQTLVKAKKPLVGHNMLMDLLYLHEKFYKPLPEKYEEFKDNIHFLFPVLLDTKNIAKATWKEFQFPQVSYLLELYEMLCSVVNPTDQLCPEIFHSDDCLRYAINKYPHEAAYDAFLCGAVLLKIAHLLLCRKTGHQTEAPTFSQYLDVLAAYVNQVNLIRGGIQKINFSGADDLTSRPPLLLATVKGWHGVTEEQIHQELRSFCRFNVRQLRMNQFLLLSNYFKDIRIVMKVYKAHPNLQISMYHRWKHSPRVTSFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7P9</accession>
    <name>A0A6I9X7P9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase accessory subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">QTRTD1</name>
        <name evidence="5" type="synonym">QTRT2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of a catalytic subunit QTRT1 and an accessory subunit QTRT2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion outer membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="5">May associate with the mitochondrion outer membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the queuine tRNA-ribosyltransferase family. QTRT2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911504.1">
        <property type="nucleotide sequence ID" value="XM_014056029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7P9"/>
    <dbReference type="GeneID" id="106540781"/>
    <dbReference type="KEGG" id="tsr:106540781"/>
    <dbReference type="CTD" id="79691"/>
    <dbReference type="OrthoDB" id="27601at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03043">
        <property type="entry name" value="QTRT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028592">
        <property type="entry name" value="QTRTD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050852">
        <property type="entry name" value="Queuine_tRNA-ribosyltrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="7">
        <location>
            <begin position="12"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="298"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911504.1"/>
        </source>
    </evidence>
    <sequence length="429" mass="48917" checksum="13F89C953F2B47BE" modified="2020-10-07" version="1">MKLNLCKIVDGCRLGTLTNLGKKEVQSMEIPGCLLYTKMGTAPHLTHDTLHSIQGLPVVVQISLTTLADLQEVLQEYKEGIGKFIGMPESILYCSLQDPAVCSPHGYNSMKTVSIWNNRGRKELTASKFMDIQKAIQPDWFQCLSDGDTISADISRKKAKKSVDRSLYFLDECLQLQENSPELKQSLMFGVIEGGDVLEERLRSARETAKRPVAGFLLDAFQGNTMTKETKLELLTSVIAELPEDKPRLIHGIGKPDEILECIKRGIDIFDSSFPYQVTERHCALCFSYDYQLNPESADIEEDEKTEVRKDDKKPKDETCSEAQEKDDENPKDETCREAQEMTSFEIHLKDRKYQDDFNPLVNGCSCYCCQNHSRAYVHHLVMTKELLAYVLLMMHNFQHYFGFFSSIRNALKEDKLDQLRKVISRQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X843</accession>
    <name>A0A6I9X843_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amelotin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AMTN</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907498.1">
        <property type="nucleotide sequence ID" value="XM_014052023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X843"/>
    <dbReference type="GeneID" id="106537780"/>
    <dbReference type="KEGG" id="tsr:106537780"/>
    <dbReference type="CTD" id="401138"/>
    <dbReference type="OrthoDB" id="9837242at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042475">
        <property type="term" value="P:odontogenesis of dentin-containing tooth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070175">
        <property type="term" value="P:positive regulation of enamel mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031501">
        <property type="entry name" value="Amelotin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36858">
        <property type="entry name" value="AMELOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36858:SF1">
        <property type="entry name" value="AMELOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15757">
        <property type="entry name" value="Amelotin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027043870" evidence="2">
        <location>
            <begin position="17"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907498.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="31152" checksum="DC9738C2FFD93C7D" modified="2020-10-07" version="1">MKIIILLLSLLGLTFSIPINHIGGILATSSSKEILRLLQRYIAIGNTPQQAQSRQRAVLPPAKLVPDPNLPGNQPPNMNFPVISLPQNQVFPIDMNYLSTLLGILSTMQTLPIVGGGMTVPQQLPPGQAMLPIIVTQMGPQGAVLSSEEMSPSQFFTGLLVPGMQGAMFPSGQAEPPPDGQDGFLPVGQANPEGTAAGIQKVFPTATDGLSEIPGELYPTPSGFRQPDAGTSGMFVEPTADINMEPSELREPPTSLVKFDMGNMQSPHNLSQPLVRGDSHIPMNTAASKPLNVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAH2</accession>
    <name>A0A6I9XAH2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Cytochrome c oxidase subunit 7C, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Cytochrome c oxidase polypeptide VIIc</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">LOC106540240</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits, encoded in the mitochondrial DNA, and 11 supernumerary subunits, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the cytochrome c oxidase VIIc family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910774.1">
        <property type="nucleotide sequence ID" value="XM_014055299.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910782.1">
        <property type="nucleotide sequence ID" value="XM_014055307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAH2"/>
    <dbReference type="GeneID" id="106540240"/>
    <dbReference type="KEGG" id="tsr:106540240"/>
    <dbReference type="OrthoDB" id="9974841at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.49.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.49.10">
        <property type="entry name" value="Cytochrome c oxidase subunit VIIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004202">
        <property type="entry name" value="COX7C/Cox8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036636">
        <property type="entry name" value="COX7C/Cox8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT VIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02935">
        <property type="entry name" value="COX7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81427">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013910774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013910782.1"/>
        </source>
    </evidence>
    <sequence length="63" mass="7227" checksum="3177A9012AE4116D" modified="2020-10-07" version="1">MLTTTIRRFGTSALRRMHYEDGPGKNLPFSVNNKWKLLFGTFIFTITGIGGPCFIVRHQLLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAJ5</accession>
    <name>A0A6I9XAJ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Centrosomal protein of 164 kDa isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CEP164</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911331.1">
        <property type="nucleotide sequence ID" value="XM_014055856.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540663"/>
    <dbReference type="KEGG" id="tsr:106540663"/>
    <dbReference type="CTD" id="22897"/>
    <dbReference type="OrthoDB" id="6344460at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051841">
        <property type="entry name" value="MT-Golgi_org_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18902:SF27">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 164 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18902">
        <property type="entry name" value="NUCLEAR MITOTIC APPARATUS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="395"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="507"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="594"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1053"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="700"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="772"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1258"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="507"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="526"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="630"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1053"/>
            <end position="1073"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1178"/>
            <end position="1187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013911331.1"/>
        </source>
    </evidence>
    <sequence length="1555" mass="174722" checksum="3CB77708319E8BDB" modified="2020-10-07" version="1">MVPGRLAIGKIEVIDITSDILAADKREQVGTDQGDPPFPGGTGGPQGERKAGQGIVCLCDERYRQLVIQEREKILEHGGLKKKEKKKKKAEKKKDKKERDPSRHPLEMQSEPGILPSASFYRVSSPILYSEHGSPDQEQQGSLVIQNDSLLKNPKGKISDAGDLSRLLSSPAPGKLQPLLPEKSNRTQQILADVEKILGRNASSSRSDVNHQAQQDANTEVRCAAASGILSDLEAEDVDSIHLIEPFFQMLKKPSQTIAGAMTVLALGGVKEKKLFLEGGKQGEDHGKGHAEGDGLVRKEDFLHSVEKERQLSPSAAAAAFTLISSRTLEGRGDIRYFQNETVRPLERIKESRRGKKNHSEQCQATESSPWQIETLVPHSQLLHGSVELRAKTGISGSEVSSPTPGQLTGGGNHDPKMESDSGNSISVASSLSDHLASQVLGEVDNLSWDLQSSYESEHPTKHLPSPQRPFLEAVNSQAQSSPDNQSASECYSEDQKFYQHVLHMVQQSRRTESGVNPKSLKGQKGCSQSPGSDQTVQPDMAGEGTTEMAAVEAATPEGERLKPCEGLLRDNQAGEQLGSNPVLVEGACRVGLPKVGGGTEEPSSCGKNTLKSKNEEEKEACLSHLISDVTEKKEVSEDKNWLVQGKNEDKGRLEEEQRSVAEESRQFEAEINPIKPWNVQTGGSQEEITNVEETNMYLLEEKQIRIQNLQEELQQREEEEIQMLRQQKESALPSLKTELETTRQEEEIKVELQQLHTELHSEMDAGKEKIRLAQEAALNQLKEELESFQQREREKLKKQKCLSLERIKKEAELAEQAEQMALEQEKQRALDELREKLCREKELALQELQVQLAADIQQLKTAAAEEHQKEEGSLKAPALSPGQVILTLRMEIMEAQRKEKAELQKELESVEQNVQQKRHQVAEYERELSDLLKEKRQEVEREHARELEKMQKIHQETLARIQMQHEDKEKKQKAELLAGLQDELEHMELLHNAQQEALRKTHTEELKELRQKRQEQVGKMEKKAQDAELELELRAQDAQTKAAQLRAQEEAWKKKRQQVLEEEKQLEEERNEAALAAHSRLQESQKAQENLEDTLQQLHSALVELQDQKMKLESQVELLQIQSQQLERHTSELEETVRTKQELLKKLDKEWSEATSPREKEEALRVEDLQGDGPEPSFREMTSEIPRSNEENSLLLNQVRHYISSEGASLKTAKEFLVCQTRSMRKRRTALRAAKQHWYGGLQGTPAVQDSGHSQVLEGVRRNLEEEGRQLDEMKSAMRKGQELLKKKEERLSQLESSLLEEFSDEDTIKGVACKKMVTFDLSDSEDTSSLTSADRSLHKTADLKPDVEFSPFDKIQYLTESLQRITSDLNCVLRLLSTFSSQQTLSASTQGQSLAPLARDGIPLAAYTSLARAYSATSFAPSPGLQSVWCNSSPSSATVSRQSVDNMLMEKWRKYFPGKFPLPCGGFGAQDDKLDSFITGEQVCLFQHPHFQGSKAEKPNIQRMIDANKKWLESFKHDCKSPFLPSPSHSSSSGSGLVQLGLDENNQIKVYHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC03</accession>
    <name>A0A6I9XC03_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SLIT-ROBO Rho GTPase-activating protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rho GTPase-activating protein 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SRGAP1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908708.1">
        <property type="nucleotide sequence ID" value="XM_014053233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC03"/>
    <dbReference type="GeneID" id="106538666"/>
    <dbReference type="KEGG" id="tsr:106538666"/>
    <dbReference type="CTD" id="57522"/>
    <dbReference type="OrthoDB" id="5981864at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04383">
        <property type="entry name" value="RhoGAP_srGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11955">
        <property type="entry name" value="SH3_srGAP1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000010">
        <property type="entry name" value="SLIT-ROBO Rho GTPase-activating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000006">
        <property type="entry name" value="SLIT-ROBO Rho GTPase-activating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000005">
        <property type="entry name" value="SLIT-ROBO Rho GTPase-activating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051627">
        <property type="entry name" value="SLIT-ROBO_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035648">
        <property type="entry name" value="srGAP1/2/3_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14166">
        <property type="entry name" value="SLIT-ROBO RHO GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="14">
        <location>
            <begin position="22"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="13">
        <location>
            <begin position="490"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="720"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="785"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="872"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1001"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="785"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="808"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="901"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1006"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1046"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00083891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013908708.1"/>
        </source>
    </evidence>
    <sequence length="1064" mass="121980" checksum="B04B7D1649FC60B7" modified="2020-10-07" version="1">MSSSSRFKKDKEIVAEYESQVKEIRAQLIEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRSLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRVFKKSKEIAFQLQEDLMRVLNELYTVMKTYHMYHTESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIYDLSDLIDCCDLGYHASLNRALRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPTAFCPPMKFEFQPHMGDEVSQVSAQQPVQGELMLRYQQLQSRLTTLKIENEEVKKTTEATSQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQFYFMKFREYLEGSNLITKLQAKHDSLQRTLGEGHRAEYMTTSRGRRNSHTRYQDLGQIIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGENPLADDQSNHDINSVAGVLKLYFRGLENSIFPKERFNDLISCVRTENLYERALHIRKLLLTLPRSVLILLRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPIPDIQEQVSCQAHVNEIIKTIIIHQEIIFPDSKELDGPVYEKCMAGDDYCDSPYSEHGILEEVDLDAGAEVHTSDDECDPIEAIAKFDYVGRSARELSFKKGASLLLYHRASDDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSETSSGPILEDKSSSKDMSSPTDLNSEVYLGRQCKRSELPLSGRRPGRTSDGHCPVHTPHGLTNSSMELVSPIMASHATSRAALQNHSLRTDSPERRRKPGHSSLTNISRHESLKKSDSPPIRRSTSSGQYPVFNDHKPLDPESIAQDIEETMNTALNELRELERQSSAKHAPDVVLDTLEQIKNSPTSTTSTESLSPLHNIMLRNAEPQIHRSTSSSSDTMSTFKPMVTPRMGVQLKPPALRPKPIVLPKTNPSIGPSSPSQGPADKSCTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCE1</accession>
    <name>A0A6I9XCE1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sal-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SALL1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transcriptional repressor involved in organogenesis. Plays an essential role in ureteric bud invasion during kidney development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">May associate with NuRD histone deacetylase complex (HDAC). Interacts with components of HDAC complex including HDAC1, HDAC2, RBBP4, RBPP7, MTA1 and MTA2. Interacts with CCNQ. Interacts with NSD2 (via PHD-type zinc fingers 1, 2 and 3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911522.1">
        <property type="nucleotide sequence ID" value="XM_014056047.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540797"/>
    <dbReference type="KEGG" id="tsr:106540797"/>
    <dbReference type="CTD" id="6299"/>
    <dbReference type="OrthoDB" id="8749569at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042733">
        <property type="term" value="P:embryonic digit morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003337">
        <property type="term" value="P:mesenchymal to epithelial transition involved in metanephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021772">
        <property type="term" value="P:olfactory bulb development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000708">
        <property type="entry name" value="Sal-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000689">
        <property type="entry name" value="Spalt like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000025">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000260">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000302">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000341">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000079">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000215">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF51">
        <property type="entry name" value="SAL-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="695"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="723"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="755"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="990"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1018"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1130"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1158"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="78"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="559"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="780"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="882"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1048"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1088"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="620"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="809"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="832"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="888"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1058"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013911522.1"/>
        </source>
    </evidence>
    <sequence length="1320" mass="141012" checksum="A54FB739B334A39B" modified="2020-10-07" version="1">MSRRKQAKPQHFQSDPELASQAPRNGDTQKGQPNRTPKTKDAHVCGRCCAEFFELTDLLQHKKICTKNQLVLIVNESSVSPSEAFPPSPPKDHPDERKTNTDNNAGQVEGSNVPERDDKLDKEETMDTEPSSSSVSKSTSSTSQTVNSTIASSNCSTKGTSALTTSLPQLGDLTMLGNFSVINSNVIIENLQSTKVAVAQFSQEARCNGTTTHKLAVPALMEQLLALQQQQIHQLQLIEQIRHQILLLASQNADVPTTSPNPSQSTVRASANPLSTLSSHLSQQLAAAAGLAQSLASQSASIGGLKLLSPLQLPQSNSGNTTIPSSSGLSPSISLATAAITTSSSDKVTTNTGGPPLGNPAGTVSSSPAFAISSLLSPVSNPLLPQPTSSNNSVFPNPLSNLGTTAEDLSPLSALAQQRKNKPPNLAAFETKSNSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTSWLDNKPVLSTLTPSVGLPLPPTIPSLTPFIKTEEPQPIPISHPSSSPPSSVKSDSGSIDPAAKNSNGPLADGEMCALLPSGDKAEESMPSVSSSLNHSVTSPAAESDSSNVVAFTSPLMPLMSEQFKAKFPFGGLLDGTPASETSKLQQLVENIDKKASDPNECLICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPVTENYPESMESDTGSFDEKTFDDLDAFSDENMEDCPDSSIADTPKSADASQDSLSSSPLPPEISSIAALENQMKMINAGLAEQLQASLKSVENGSVEGDIMTNDSSSVGGDMESQSAGSPAISESTSSMQALSPSNSTTDFYKSPGIDDKPLRTLPNDFANGWPVAFANGGALDLTSGNPEKMVKEEPLGMLFPFRDRGKLKNTACDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPNSNLGPAQNSSTSSSSSSVVPANSLSSLIKAEVNGFVHGPPQDHKETLPSLLPSGPLLPSATSPVLLPTLPRRTPKQHFCNACGKTFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMTFLGGNPVKFPDMFPKDLAARSGNGDASSFWNQYAAALSNGLAMKTNEISVIQNGGIAPVPGSLGNGSSSPLSGLTGSLEKFQDSEPNAPLAGLERMAGSENRTSFRLTRFVEDSKEIVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCF2</accession>
    <name>A0A6I9XCF2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TRAF3-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TRAF3IP1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAF3IP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913264.1">
        <property type="nucleotide sequence ID" value="XM_014057789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCF2"/>
    <dbReference type="GeneID" id="106542140"/>
    <dbReference type="KEGG" id="tsr:106542140"/>
    <dbReference type="CTD" id="26146"/>
    <dbReference type="OrthoDB" id="10258914at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.50:FF:000001">
        <property type="entry name" value="TRAF3-interacting protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.50">
        <property type="entry name" value="Microtubule-binding protein MIP-T3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018799">
        <property type="entry name" value="TRAF3IP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041476">
        <property type="entry name" value="TRAF3IP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040468">
        <property type="entry name" value="TRAF3IP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042576">
        <property type="entry name" value="TRAF3IP1_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31363">
        <property type="entry name" value="TRAF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31363:SF0">
        <property type="entry name" value="TRAF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10243">
        <property type="entry name" value="MIP-T3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17749">
        <property type="entry name" value="MIP-T3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAF3-interacting protein 1 N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF3-interacting protein 1 C-terminal" evidence="13">
        <location>
            <begin position="443"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="133"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="514"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="150"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="208"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013913264.1"/>
        </source>
    </evidence>
    <sequence length="615" mass="71419" checksum="57047575E908E497" modified="2020-10-07" version="1">MQGSVIRRTQELLGRVIRKPPLTERLLSKPPFRYLHDVIGEVIRATGFMKGLYTEAEMKSDNVKDKDAKIIFLQKAIDVVIIVTGEHLAVKPARVVAGHEPEKTNEFLQAIAKCCLNKLSSDEAVQKVLAEEKSFKEKTPSSSKSQNTESKTEESRYHREGRNDSEIKDKSTIQDQKVKEELKEEESKQRENEKYKDYENNPNNFDKTVNESEKHEKERIKNRLSKSAREPEKIRDKEKVEVEGEKECDKERDKERKQHEGGKEKERLREKEKPRDRDKDSEKVRDRYKEKDKRREKGKDIETTREERKDKMDKKSTGSEEIIAKKLAEKSINKDQAEFDKENENSARLSKQHATKGPKQRNKTLSEGKRLPRPSSARPAPPRIKHQQSTEILIPERNGSAKMVSNVIIDKEEEEDDQFIVKAEQQLPEITELKMEATVELEGDEKHGSLVQRILETKKDYELSQLQKSAEKEKPLLSEAAKRKEKDLVTKEIEKFRGSIQALCRSALPLGKIMDYIQEDVDAMKNELQTWQNENKELEETLRKEQGITDGVVEPLKIELTELDQLIKDEEDKIYTKKANVLKNNEKIQKMVHIINSRRINILTRSSVEPKLNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCG5</accession>
    <name>A0A6I9XCG5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DNA mismatch repair protein Msh3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MSH3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908848.1">
        <property type="nucleotide sequence ID" value="XM_014053373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCG5"/>
    <dbReference type="GeneID" id="106538764"/>
    <dbReference type="KEGG" id="tsr:106538764"/>
    <dbReference type="CTD" id="4437"/>
    <dbReference type="OrthoDB" id="121051at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016447">
        <property type="term" value="P:somatic recombination of immunoglobulin gene segments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein Msh3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000870">
        <property type="entry name" value="MutS protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF122">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="9">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013908848.1"/>
        </source>
    </evidence>
    <sequence length="693" mass="78761" checksum="AA70E1D203432DB5" modified="2020-10-07" version="1">MRAFAIVQLYVINSMVKLKMSIFYSLQDDRIRIERMKNEHFEYGSAFKMITDFYGSQILDGTGSQALSQILKFDKSVLCSLAAVILYLKEFNLEKILYNPSNFKKLSNEDEYMTLNGATLKNLEILRNQTDMKTKGSLFFILDHTKTPFGRRKLKKWVTQPLLKSSEINSRLDAVSEILFSESSVLGQIMSLLHKVPDVERGICSIYHKKCSVQEFYLVVNTLSNLETAVKALVPAIQVQVRASLLHKSLLEIPELLSSTKKYLSKLNEEAAKIGDKTELFKDLDDFPLIRKRRGEIQTVLSQIKNHIQDVRRMLNSSSVDYISVSGQEFQIEVKNSVVSSVPLDWIKVSSTKVVSRFHTPFIVENYRKLNQLREQLVLDFNSEWLCFLDHFNEHYHALSQAVGHLATVDCVFSLAEAAKQGDYCRPVIIDEKSEIMIKNGKHPVIDMLLGEQQQYVPNDTFLSEDSKRVMIITGPNMGGKSSYIKQVALITIMAQIGSYVPAEEVRIGIVDGIFTRMGASDNVFKGRSTFMEELTDTAAIIQKATSHSLVILDELGRGTSTHDGIAIAYATLEHFIKNVGSLTLFVTHYPPLCELERAYPTHVENYHMSFLVNEDYSKPEDDFEEQNHLDCVTFLYQITKEVAARSYGLNVAKLADVPKEILKKAAYKSKELERSADVKRSHVNYSKVNNRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDA4</accession>
    <name>A0A6I9XDA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Potassium voltage-gated channel subfamily A member 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KCNA10</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Voltage-gated potassium ion channel that mediates K(+) permeability of excitable membranes. When opened in response to the voltage difference across the membrane, KCNA10 channel selectively allows the flow of potassium ions across the membrane down their electrochemical gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.8/KCNA10 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912201.1">
        <property type="nucleotide sequence ID" value="XM_014056726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDA4"/>
    <dbReference type="GeneID" id="106541328"/>
    <dbReference type="KEGG" id="tsr:106541328"/>
    <dbReference type="CTD" id="3744"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000033">
        <property type="entry name" value="potassium voltage-gated channel subfamily A member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000012">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF44">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="17">
        <location>
            <begin position="90"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013912201.1"/>
        </source>
    </evidence>
    <sequence length="513" mass="58626" checksum="0D7C860284E11C6E" modified="2020-10-07" version="1">MDVSTWKEMEVALVNFDNTDEIMEEPCFSSDLNAAEQLHKGRSSCASLLSNWRILINNENTNNETIFSKLSAEFCDHLGAENGGMDEGDQRVIINIAGLKFETQLKTLHQFPDTLLGDPEKRIHYFDSMRNEYFFDRNRPSFDGILYYYQSGGKIRRPANVPIDIFADEITFYELGEEAMDQFREDEGYIKDPITLLPSNDFHRQFWLLFEYPESSSAARGVALVSVLVIVISIIIFCVETLPEFRDEKELKYESNNVTNVVMASNTFTDPFFIIETACIIWFSFELVVRFIVCPSKTEFFKNIMNIIDIVSIIPYFVTLTTELIQHSELNGQQNMSLAILRIIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLVFFLFIGVILFSSAIYFAEVDEPQSHFSSIPDGFWWAVVTMTTVGYGDMCPTTPGGKIVGTLCAIAGVLTIALPVPVIVSNFNYFYHRETENEERQVLPREIEKILTRAVTGNGSIDSLNITDENFSQEKKKMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDD4</accession>
    <name>A0A6I9XDD4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transforming growth factor beta receptor type 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TGFBR3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912226.1">
        <property type="nucleotide sequence ID" value="XM_014056751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDD4"/>
    <dbReference type="GeneID" id="106541340"/>
    <dbReference type="KEGG" id="tsr:106541340"/>
    <dbReference type="CTD" id="7049"/>
    <dbReference type="OrthoDB" id="6420824at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005539">
        <property type="term" value="F:glycosaminoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050431">
        <property type="term" value="F:transforming growth factor beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005024">
        <property type="term" value="F:transforming growth factor beta receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005114">
        <property type="term" value="F:type II transforming growth factor beta receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001837">
        <property type="term" value="P:epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017015">
        <property type="term" value="P:regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058899">
        <property type="entry name" value="TGFBR3/Endoglin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF7">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA RECEPTOR TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26060">
        <property type="entry name" value="TGFBR3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="531"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="12">
        <location>
            <begin position="199"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="561"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="582"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912226.1"/>
        </source>
    </evidence>
    <sequence length="597" mass="66248" checksum="4A766B9A18200C02" modified="2020-10-07" version="1">AFQVDIIIDIRPSRPDTTLVRNLVLILKCRKSVNWVIKSHDVQGRLEAITSNGVGFGKETERSMTMSKTIVFDMPSSHENLIAWAYEHGHYPVTTYTKAPLANRFHLQLVDAEEMNDEEEEDFLPPELKELLHGIKSPPTSRLDQDMHFSFLHENNQGSLPPADTVDPIIASHGFEQLLPRDPEPEEVQGSVDVAMSVICNDTSMMVAVAKDSLEASGFIGTQLSLLDPTCRAKSNRTHFILESSLHDCGTRQIPYPLDNVVYLNSIVIQVSPSTEASGWPIDYEDMESGDNGFPGDTDESDITFFSRPVIVVFNCTFHQPRDSKVSRFWPPEVRVGNATFGMELYETELFRAPVQGFFSVADNSQIFVEISLTKAERSLGFVIQTCFVSPNSYPDRMSEYTIIENVCPKEESVRFFNVPKANFPVPNAHTDKKRFSFLFKSTFNSSLLFLHCEVTLCTKREKDIPGLAQCILPDEACAFLNVDMIFAMMQNKKTFTKPLAVITKGRPEDKSSHLNPSEGSASVVYGLDTLTVVGIAFAAFVIGALLTGALWFIYSRTGDPEGRQQVPTSPPPSENSSAGHSIGSTQSTPCSSSSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDU9</accession>
    <name>A0A6I9XDU9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Guanine nucleotide-binding protein G(T) subunit alpha-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GNAT1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909308.1">
        <property type="nucleotide sequence ID" value="XM_014053833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDU9"/>
    <dbReference type="GeneID" id="106539132"/>
    <dbReference type="KEGG" id="tsr:106539132"/>
    <dbReference type="CTD" id="2779"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050908">
        <property type="term" value="P:detection of light stimulus involved in visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007603">
        <property type="term" value="P:phototransduction, visible light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000256">
        <property type="entry name" value="Guanine nucleotide-binding protein G(t) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF67">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="9" id="KW-0844">Vision</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="139"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="164"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="233"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013909308.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="36324" checksum="C33C18A96DD1F05B" modified="2020-10-07" version="1">MGSGAGESGKSTIVKQMKIIHKDGYSLEECLEFISVIYSNTLQSMLSIVRAMGILNIQYGDPARQDDSRMLLHLADTIDEGTMPKEMSDIIIRLWKDTGIQACFDRASEFQLNDSAGYYLNDVARLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLHFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDDVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFQEKIKKAHLSICFPDYEGTNTYEDAGAYIKVQFLELNIRRDVKEIYSHMTCATDTENVKFVFDAVTDIIIKENLKDCGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDV9</accession>
    <name>A0A6I9XDV9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Interleukin-27 subunit beta-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106542555</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type I cytokine receptor family. Type 3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913809.1">
        <property type="nucleotide sequence ID" value="XM_014058334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDV9"/>
    <dbReference type="GeneID" id="106542555"/>
    <dbReference type="KEGG" id="tsr:106542555"/>
    <dbReference type="OrthoDB" id="6381660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000136">
        <property type="entry name" value="Ciliary neurotrophic factor receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056621">
        <property type="entry name" value="FN3_IL27B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003530">
        <property type="entry name" value="Hematopoietin_rcpt_L_F3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053073">
        <property type="entry name" value="IL11/IL27_subunit_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48483">
        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48483:SF2">
        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24031">
        <property type="entry name" value="FN3_IL27B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01354">
        <property type="entry name" value="HEMATOPO_REC_L_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027100228" evidence="6">
        <location>
            <begin position="20"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="37"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="208"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013913809.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="34441" checksum="F03478C7611E1164" modified="2020-10-07" version="1">MQWLLSFLLFLLSGLIVLASTAWTSEEPIDHQYARLGATTVVLPCPVPEGRSRVAWKNHMSSETALDGVQPDGSLVLQNASLAQEGEYRCQDASTGQTLRRIRLRLGYPPKKPSIHCRSISYPVVNCSWTLESDTLLPTSFFASYRHGLMGDVHQCKQLVAESHSCSLAGLQMFSTDPYMLNVTAVNPLGSASDYFLIFLDQIIQPDPPENVMVSPIQGKKKTLLVQWKPPSSWLYPEYFPLQYIIRYSRDGSRFSRTTRPIEQTSFTLTGIRSGVTYHIQVAAKDFLDNGEYSAWSLPASGASWQPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDW9</accession>
    <name>A0A6I9XDW9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UPF0690 protein C1orf52 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106542565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0690 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913819.1">
        <property type="nucleotide sequence ID" value="XM_014058344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDW9"/>
    <dbReference type="GeneID" id="106542565"/>
    <dbReference type="KEGG" id="tsr:106542565"/>
    <dbReference type="OrthoDB" id="1906229at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029089">
        <property type="entry name" value="DUF4660"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31833">
        <property type="entry name" value="UPF0690 PROTEIN C1ORF52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31833:SF2">
        <property type="entry name" value="UPF0690 PROTEIN C1ORF52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15559">
        <property type="entry name" value="DUF4660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913819.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="20818" checksum="207F5C9F1F9F6FA7" modified="2020-10-07" version="1">MAAAEGKDPLSFFAAYGSDNSSSGGSDAEDEEGSGRGSKGSSQQSKNGTSEGKPRLPGPDELFRSVNRPAFLYNPLHKEIDWESRVLRAPEEPPKEFKAWTTNAVPPPDTYTIKEKKPPPPPERDMAIKWSNIYEDNGDDAPRHVNNVHFLPEEEQETVESDGEKDEPSSVKKRKLNPDEQPKKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFI2</accession>
    <name>A0A6I9XFI2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Receptor tyrosine-protein kinase erbB-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912637.1">
        <property type="nucleotide sequence ID" value="XM_014057162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFI2"/>
    <dbReference type="GeneID" id="106541654"/>
    <dbReference type="KEGG" id="tsr:106541654"/>
    <dbReference type="OrthoDB" id="6219513at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912637.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="28365" checksum="CBE0F24F175AF7D4" modified="2020-10-07" version="1">MSGNQFIYRDGSLSTEQGVPLPYRTAIPGIAPEASIRQGGAEICDDTCCNGTLRKQVASLSQEDSVTQRYSADPTVFIPERGPRTELDEEGYMTPMPEKGKTDHLNPVEENPFVSRWKNGDLQALDNPDYHNSQTGQPKAEDEYVSEPLYLNTFASTFENAEYLKKNGLPLPEKPKRAFDNPGYWNHSLPPRSTLQHPDYLQEYSTKYFYKQNGRIRPTVAENPEYLSEFSLKPGMMLPPPPYRHRNTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFZ3</accession>
    <name>A0A6I9XFZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial coenzyme A transporter SLC25A42</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Solute carrier family 25 member 42</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SLC25A42</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial carrier mediating the transport of coenzyme A (CoA) in mitochondria in exchange for intramitochondrial (deoxy)adenine nucleotides and adenosine 3',5'-diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3'-dephospho-CoA(in) + ADP(out) = 3'-dephospho-CoA(out) + ADP(in)</text>
            <dbReference type="Rhea" id="RHEA:72843"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ADP(out) + CoA(in) = ADP(in) + CoA(out)</text>
            <dbReference type="Rhea" id="RHEA:72839"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>AMP(in) + ADP(out) = AMP(out) + ADP(in)</text>
            <dbReference type="Rhea" id="RHEA:72851"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>adenosine 3',5'-bisphosphate(in) + ADP(out) = adenosine 3',5'-bisphosphate(out) + ADP(in)</text>
            <dbReference type="Rhea" id="RHEA:72847"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dADP(in) + ADP(out) = dADP(out) + ADP(in)</text>
            <dbReference type="Rhea" id="RHEA:72855"/>
            <dbReference type="ChEBI" id="CHEBI:57667"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913081.1">
        <property type="nucleotide sequence ID" value="XM_014057606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFZ3"/>
    <dbReference type="GeneID" id="106542008"/>
    <dbReference type="KEGG" id="tsr:106542008"/>
    <dbReference type="CTD" id="284439"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000014">
        <property type="entry name" value="mitochondrial coenzyme A transporter SLC25A42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002167">
        <property type="entry name" value="GDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00928">
        <property type="entry name" value="GRAVESDC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 19 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="19">
        <location>
            <begin position="39"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="19">
        <location>
            <begin position="137"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="19">
        <location>
            <begin position="232"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013913081.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="36898" checksum="A1B45B7BED3F7BC4" modified="2020-10-07" version="1">MDNGRAVEGQVGLKQKDAEIRVHGRILPETQTQNAEEKKSVIHSLLSGALAGAVAKTAVAPLDRTKIIFQVSSNKFSAKEAYRLIYRTYLSEGFFSLWRGNSATMVRVVPYAAIQFCAHEEYKQLLGSYYGFQGKALTPLPRFIAGSLAGITASMLTYPLDLVRARMAVTPKEMYSNIIHVFMRISHEEGLKTLYRGFSPTILGVIPYAGLSFFTYETLKKLHADHSNGREPYPLERLAFGACAGLIGQSASYPLDVVRRRMQTAGALGYSSQSILPTMRDIIREAGLVRGLYKGLSMNWVKGPVAVGISFTTFDLMQILLRKWERSRNVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFZ8</accession>
    <name>A0A6I9XFZ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106541787</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912797.1">
        <property type="nucleotide sequence ID" value="XM_014057322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFZ8"/>
    <dbReference type="GeneID" id="106541787"/>
    <dbReference type="KEGG" id="tsr:106541787"/>
    <dbReference type="OrthoDB" id="9045351at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="299"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013912797.1"/>
        </source>
    </evidence>
    <sequence length="542" mass="60362" checksum="4E1EBDC26BAC0FDB" modified="2020-10-07" version="1">MTLGYNIYEILFNARITYDAMMDFLSMGQDNVPNYSCGRPKNLLAVLEGTDSELFSHVSSMLGIYKIPQFVKTAFQSSYLSPVLSKRIWGRCIEKENWEMPPHNVVADILSEDSHSISTAIQAVSLVLHAVRSSRFHFPQVVQPWQLHPFLRNFQLYNLSVDNVYLDDDGNPAADLDIINWAMFPNKSTVGVKIGNIERGASSEVKVSINQSAIKWPTSFNQKAPFSRCAKSCLPGYSKVKREGAPVCCYDCSSCIEGTISRQEDEAYCEKCPDEQHSSKNRDQCVPKVLSYLSYQETLGLSLALIASFLSLTTAFVLGIFIIHRKTPIVRANNRDLTYILLVSLLLSFLTSLLFIGHPQKMTCLLRQTAFSIVFSVAVSSLLAKTVMVVVAFLATKPGSSMRKWLGKSLPNSIVLSCSGIQIGICIIWLGISPPFPDSDFHSQPEHILLQCNEGSVTMFYSALGYMGFLAAICFFVAFLARKLPGTFNEAKLITFSMLVFCSVWISFVPTYQSTNGKYLVALLHLCTIPQLNAICDEDCDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGW9</accession>
    <name>A0A6I9XGW9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">RNA-binding protein NOB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NOB1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOB1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913361.1">
        <property type="nucleotide sequence ID" value="XM_014057886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGW9"/>
    <dbReference type="GeneID" id="106542220"/>
    <dbReference type="KEGG" id="tsr:106542220"/>
    <dbReference type="CTD" id="28987"/>
    <dbReference type="OrthoDB" id="446759at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017117">
        <property type="entry name" value="Nob1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033461">
        <property type="entry name" value="WRNPLPNID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15017">
        <property type="entry name" value="WRNPLPNID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037125">
        <property type="entry name" value="D-site_20S_pre-rRNA_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="12">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Putative WW-binding" evidence="11">
        <location>
            <begin position="102"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="10">
        <location>
            <begin position="205"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="264"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037125-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF15017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913361.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="39886" checksum="7E5B7B8D89B27472" modified="2020-10-07" version="1">MEEQSGRMSLSEFSKQTGDFRSLSAADIQVLALTLQLEAEHGGGRPLRTEPRPQVQLSLTNRHPEAPVDVPGFHVPLKTKCPGNERIPLEAETDPADPTETEFSSFLFWRSPLPNIEEDLEGLLKADPLLLETGDLEGQESSEEEESGTEDGWITPRNIEQIQKSTCWGSEPAEVQVGCITTDFAMQNVLMQMGLHVLAFNGLLIQHARSYILRCHGCFRTTSNMTRLFCPHCGNKTLKKVAVTVKDDGSFHLHFSQNPKVLNPRGLRHSLPAPQGGKHASNPHLTEDQRFPQQRLSRKARQKTKAFDPDYVAGVSPFAEHDIHSRAANLQIRDTALGAGRRRLNPNMSRKKFMKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH58</accession>
    <name>A0A6I9XH58_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Magnesium transporter NIPA4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NIPAL4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIPA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915044.1">
        <property type="nucleotide sequence ID" value="XM_014059569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH58"/>
    <dbReference type="GeneID" id="106543529"/>
    <dbReference type="KEGG" id="tsr:106543529"/>
    <dbReference type="CTD" id="348938"/>
    <dbReference type="OrthoDB" id="6428174at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF7">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013915044.1"/>
        </source>
    </evidence>
    <sequence length="402" mass="43933" checksum="B697AC2419410527" modified="2020-10-07" version="1">MEVPEPLWVNRSCVSGSLITLACLNHHAVCQIIKNVSFPVSPHINLNLSSNWMVQVEDKYGFYIGLGLAIFSSFLIGTSVILKKKGLRRLVEKGGTRAGDGGHGYLRDWLWWAGLITMGGGEAANFAAYAFAAATIVTPLGALSVLISAILSSYFLGERLNLLGKLGCMLSIVGSTILVIHAPEEEEITTLGELLSKLKEPGFLVYASILLVISLVLICFLAPRYGKTNILIYLSICSVIGAFSVSSVKGLGIAIKSFFTHLPVLQNPLTWILVFTLVASIATQINYLNKALDIFNTSMVFSIYYVLFTTIVITTSVILFKEWVTMSVKDIIGTVCGFLTIILGVFLLHAFKDMDVSLKNLPQALQTSKEPPAIKDDNNTLIELDNPDTKDQNKPKVFMIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHA8</accession>
    <name>A0A6I9XHA8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MAP3K7 C-terminal-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MAP3K7CL</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913486.1">
        <property type="nucleotide sequence ID" value="XM_014058011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHA8"/>
    <dbReference type="GeneID" id="106542321"/>
    <dbReference type="KEGG" id="tsr:106542321"/>
    <dbReference type="CTD" id="56911"/>
    <dbReference type="OrthoDB" id="8866809at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042800">
        <property type="entry name" value="Map3k7cl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47140">
        <property type="entry name" value="MAP3K7 C-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47140:SF1">
        <property type="entry name" value="MAP3K7 C-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913486.1"/>
        </source>
    </evidence>
    <sequence length="109" mass="12524" checksum="90A364F7D255B096" modified="2020-10-07" version="1">MQQQDSSQVKHTTRHIGSSAHMITTARVPADKRIRIAFSLNDSPGNNLQKKELITRLDEAEKENRDAAQLTKEYADLTDENRTLKLTQTQCVEQLEKLRIQYQKRQGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI63</accession>
    <name>A0A6I9XI63_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Gamma-aminobutyric acid receptor-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GABARAP</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913607.1">
        <property type="nucleotide sequence ID" value="XM_014058132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI63"/>
    <dbReference type="SMR" id="A0A6I9XI63"/>
    <dbReference type="GeneID" id="106542412"/>
    <dbReference type="KEGG" id="tsr:106542412"/>
    <dbReference type="CTD" id="11337"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17232">
        <property type="entry name" value="Ubl_ATG8_GABARAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000037">
        <property type="entry name" value="Gamma-aminobutyric acid receptor-associated protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="10">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013913607.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="14023" checksum="483E9B84B8A51C1E" modified="2020-10-07" version="1">MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYGSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIB7</accession>
    <name>A0A6I9XIB7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thyroid adenoma-associated protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106542548</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Together with methyltransferase FTSJ1, methylates the 2'-O-ribose of nucleotides at position 32 of the anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913801.1">
        <property type="nucleotide sequence ID" value="XM_014058326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIB7"/>
    <dbReference type="GeneID" id="106542548"/>
    <dbReference type="KEGG" id="tsr:106542548"/>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="5">
        <location>
            <begin position="364"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="4">
        <location>
            <begin position="773"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="6">
        <location>
            <begin position="1045"/>
            <end position="1135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913801.1"/>
        </source>
    </evidence>
    <sequence length="1147" mass="128249" checksum="0D13F17DAE4296A7" modified="2020-10-07" version="1">MKLLQTVAATFDLLLQDSNSSQVEWHYVTIKVCLHLFKRMSEEIQPLVWEETDHREMLQKILRSLVHTIMDQSACQDNRLLAGTTVSMMVNTAAEVEAGAKTIWAFYPLINLNAQRTEEHKGGCQFGALWIPAFILNPGGLDTLVMTRGLLTCCKKEILTCLLDGTPPKCLLLDVLFPAVLDVIEDPTSYPYYSYQVFSLWLQRVRESLDDLWKVKESLLLDDNSELLQKLSKIVWNNAESPTEGVTDFVHSSFRILLEIYSLECDQFGDPERALFREYLQKVIFMPWEARARYFALSAILPHLGPQKVLEIYPDLPQHLFNCLSTNRLCPVASDLYKTLLGLQRKAWTEVEDEVSEEHLAHKWSSCWLPTFSSALTSSDPFLQSNTSSHLLGRTLRVFPASYALLAMRFGGRDASQLRAWVSLLNVQKTLAGDLLTDEKTLKRLTFCLFSKEETVRLAALGFLCSAPKTSQVLSEREIQLLKEFLPLNLSCDSSSFRQLLQSTVKKALVRMRDSSLAALRQWKVNQMESLARKDQERGLLRAVEFVDWLLQLCTASLMPSSNYQRRKTALLLLAAILETFTDTWRPERKKGQPPRNMATLLCWSRNKGSWDFFSTGNTLALLSCLQDSTNEIRDLASELLNSYFPPDLPASAAVALFGWALEAISSPRVQDSEAGAAVMKTLLPKLDRHILKPPLLETEKEPSMPCKYLSFLEHLLNLLRKHFVMASRDLLQAAHTTPIHGVILALRRCLLEEPEVVSSMLEAQKMPHWRLLFQRLVRTVRDISNLLLSVLQSHQASSTDQHASSPSFADMGNAIRSLIRLGKGLEEEEEDMVLLSEDHSLILTCCWVSVKEIGLLLGGLTEKVLPQPVSGGCEPLLPLQVVHMASKVFQDILLKCRHWGAVEGCSMGFTKFCTTLLNHPSPELQTIPRTMLTQGLALLSSSRGSSITRRAAGFPMLFLCIVTGEDPASSRPLLTHCIQTLLALASQPISHSSEQTVDLPQVSAVHVLQTLVRGSNLGPALQPHITSLMVLVLQALSSPSWAMRNAANQLFGALSVRLLGQKWSPEDSRAKDGVSPEALFGRHVHLRSVLLGELTLAVEVSVSEGPRRGTFHLCPSLYAVLTFLAKLQPSGDTQDRGDFGVTEMPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIE5</accession>
    <name>A0A6I9XIE5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Testis-specific gene 10 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TSGA10</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP135/TSGA10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915539.1">
        <property type="nucleotide sequence ID" value="XM_014060064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIE5"/>
    <dbReference type="GeneID" id="106543956"/>
    <dbReference type="KEGG" id="tsr:106543956"/>
    <dbReference type="CTD" id="80705"/>
    <dbReference type="OrthoDB" id="10254663at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051877">
        <property type="entry name" value="Centriole_BasalBody_StrucProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544">
        <property type="entry name" value="CENTROSOMAL PROTEIN CEP135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544:SF2">
        <property type="entry name" value="TESTIS SPECIFIC 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="120"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="226"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="306"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="586"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013915539.1"/>
        </source>
    </evidence>
    <sequence length="676" mass="78770" checksum="3752437B714B22A5" modified="2020-10-07" version="1">MTSRKCPGRRRKNTEFIAKRITTREELALRGPLIESDRTSKTEDRFRRKDKIIQELLLEIEHLKTENLDLEKHVRKILNSKDKVTTQVDSLTSENEYLCKELAHVDKIAEQLEKEREFVLDSAGQELSEAKSQIKFQQNTIKKLEHTVSILKSATLDVEKSDGKLNSFVKTVEEEKDYYKSEVEKLKKMYRSSSPKRSPSCSITKISSPIQGKQSDKECLQILIERDEFKTMLEKYQRHITELQGNIKVLMAERDKAVCLYDQAQEEVNQQRKELMKIPKASTTAVTVQAVLRRVETERDAAISDFRRMAAERDSLRERLKIAQDTALNEKAHLEHRIEELELNIQSLDNERLEQLSKIELLKDAIESIEREMKILGRRASDSENELGRQKAANTSLSILNEKIEHNFTEAERQLSKKKHELQLTQEKNMCLEGKIEQLSKESLEQQGHICTLKDTINEMEAEKDSLQDLLEDKREKILTLEESLAIQEKTISDLKGILSGMEHSSKHSTEALCKYEQDIGRLHQLLDDANTELNQTCREKDMLTREHSKLKEQLCAFKQENQLLFEKLSKSQNDFDDMKLKIEDFQRLKSLLNSKEKDNEDLMENFHMIREQADRWESKLHQMEKDYNCIQMELLSAESENHRLKEQAESHEIAIGQHLASEKAYKSQISTLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJF8</accession>
    <name>A0A6I9XJF8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Omwaprin-a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106544219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915904.1">
        <property type="nucleotide sequence ID" value="XM_014060429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJF8"/>
    <dbReference type="GeneID" id="106544219"/>
    <dbReference type="KEGG" id="tsr:106544219"/>
    <dbReference type="OrthoDB" id="9425681at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026968098" evidence="1">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="2">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915904.1"/>
        </source>
    </evidence>
    <sequence length="83" mass="9253" checksum="43BE25533983DFB8" modified="2020-10-07" version="1">MKLLTTFLFVILLALWMGFPSTTGSDLPAGKQSPEKPGECLPDPCICNTPTVYKCWGDNDCEGRKKCCRYCCTMECRDPRLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKB0</accession>
    <name>A0A6I9XKB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pentraxin family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106543061</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the pentraxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914441.1">
        <property type="nucleotide sequence ID" value="XM_014058966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKB0"/>
    <dbReference type="GeneID" id="106543061"/>
    <dbReference type="KEGG" id="tsr:106543061"/>
    <dbReference type="OrthoDB" id="547680at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001849">
        <property type="term" value="F:complement component C1q complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00152">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000070">
        <property type="entry name" value="Serum amyloid P-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051005">
        <property type="entry name" value="Pentraxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869:SF7">
        <property type="entry name" value="C-REACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869">
        <property type="entry name" value="C-REACTIVE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0011">Acute phase</keyword>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pentraxin (PTX)" evidence="11">
        <location>
            <begin position="31"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914441.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="26605" checksum="C9A4F181753B8CB9" modified="2020-10-07" version="1">MKSRFPHLSENYFITTDAIGAMATATNTDLHKKVFIFPAATNKAFVRLNSTLQHPLTDLTVCLRYHSPLFSSFPLFSYATRSKDNDFLIFKPDDKKYSVYLGGDKLDFTVPVRTIPAWEHICVSWNTWDGLVHFWRNGELLPRVGMRKGYRISHEASIILGQEQDEFGGGFDIRQCFMGEMTEVNMWSRVLTTDEIQLVMREEPVPDNLINWRSLHYTAQDYVLVTNTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMN4</accession>
    <name>A0A6I9XMN4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interleukin-1 receptor-associated kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">IRAK1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915181.1">
        <property type="nucleotide sequence ID" value="XM_014059706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMN4"/>
    <dbReference type="GeneID" id="106543646"/>
    <dbReference type="KEGG" id="tsr:106543646"/>
    <dbReference type="CTD" id="3654"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071345">
        <property type="term" value="P:cellular response to cytokine stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000027">
        <property type="entry name" value="Interleukin-1 receptor-associated kinase 1 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000424">
        <property type="entry name" value="Putative interleukin-1 receptor-associated kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF939">
        <property type="entry name" value="INTERLEUKIN 1 RECEPTOR ASSOCIATED KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="207"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="101"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="524"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="604"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013915181.1"/>
        </source>
    </evidence>
    <sequence length="629" mass="70038" checksum="5906AD92C3B6F4C4" modified="2020-10-07" version="1">MAGCGSNRLLYDLPPAVMCKFYEIMDALDRAEWERFASRIVRNQVELRLYEKMDGRTHNLMWTWMNRNAYVDDLIQILDDLQLYRARDIIFSGRLLPCSQKASPVPSQKPSTFPKPPPAPDPGGRGSCKIITTMWEPPPKPSPPEPDHSNPQLPSRVSTPRSPFSSIASSTLPSPPTQSDPQGLESLPSTCPFEWQLEELRQATDDFSEHLKIGEGGFGCVYQARLRNTVYAVKRLKEEAELDWTIIKNSFVTEVEKLSRFRHPNIVEFAGCCAEQGCFCLVYVFLPKGSLEDHLHGQVDACLSWTQRLHVALGTARAIQFLHSDSPSLIHGDVKSSNILLDAALNPRLADFGLARFSRRPKQGGISSSLGQTQTVRGTLAYLPPEYVRTGMLSTAIDIFSFGVVLLELLTGRRPMEVDSRGHSKYLKDLINDEEEQEVVSTDTTSSSHTKAAQLGSHLYQKHVDLRCGPCPEELGKTLGKLACCCLHPRAKRRPSMAEVYQELEGLQLLLCGKSSAFLLLPPQQPQAPQEDYYSSTPLNEPEESDESLEANSKLLSRPRVTRESVSIGQLGNSAPQIVINPARRRMMERLALYHEGEIDSLGVLASTPSGEGAIAEPPREPEESDEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNR7</accession>
    <name>A0A6I9XNR7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Creatine kinase U-type, mitochondrial</fullName>
            <ecNumber evidence="3">2.7.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Acidic-type mitochondrial creatine kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquitous mitochondrial creatine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CKMT1A</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Exists as an octamer composed of four MTCK homodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17 19">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013917609.1">
        <property type="nucleotide sequence ID" value="XM_014062134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNR7"/>
    <dbReference type="GeneID" id="106545537"/>
    <dbReference type="KEGG" id="tsr:106545537"/>
    <dbReference type="CTD" id="548596"/>
    <dbReference type="OrthoDB" id="430219at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000002">
        <property type="entry name" value="creatine kinase S-type, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000002">
        <property type="entry name" value="Creatine kinase S-type, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF24">
        <property type="entry name" value="CREATINE KINASE U-TYPE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 18 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="20">
        <location>
            <begin position="47"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="21">
        <location>
            <begin position="160"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="163"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="327"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="355"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013917609.1"/>
        </source>
    </evidence>
    <sequence length="419" mass="47051" checksum="0CE073E9BC356F05" modified="2020-10-07" version="1">MSSTFARALSARRSAALLAAVGAAGSLGAGFLFSRDSVGLSERTKRRLYPPSAEYPDLRKHNNCMASNLTPAIYTRLCDKATPAGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEESYEVFAELFDPVIEERHNGYNPRTMKHPTDLEARKIKGGHFDERYVLSSRVRTGRSIRGLSLPPACSRAERREVEKVTAEALSGLTGELAGKYYRLSEMTDKEQEQLIEDHFLFDKPVSPLLTAAGMARDWPDARGIWHNHEKTFLVWINEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDHRLSKILENLRLQKRGTGGVDTAATGNIFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPAPISQSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ51</accession>
    <name>A0A6I9XQ51_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome quality control complex subunit NEMF</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nuclear export mediator factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NEMF</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the ribosome quality control complex (RQC), composed of the E3 ubiquitin ligase LTN1, TCF25 and NEMF associated with the 60S ribosomal subunit. The complex probably also contains VCP/p97 and its ubiquitin-binding cofactors. Interacts (via its N-terminus) with XPO1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918164.1">
        <property type="nucleotide sequence ID" value="XM_014062689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ51"/>
    <dbReference type="GeneID" id="106545957"/>
    <dbReference type="KEGG" id="tsr:106545957"/>
    <dbReference type="CTD" id="9147"/>
    <dbReference type="OrthoDB" id="207084at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140708">
        <property type="term" value="P:CAT tailing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000001">
        <property type="entry name" value="Nuclear export mediator factor Nemf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="12">
        <location>
            <begin position="525"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="13">
        <location>
            <begin position="960"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="416"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="672"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="792"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="295"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="687"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="714"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="741"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="827"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="838"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="856"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013918164.1"/>
        </source>
    </evidence>
    <sequence length="1061" mass="121064" checksum="D84A75E42344476C" modified="2020-10-07" version="1">MKSRFSTVDIKAVIAELRHSLLGMRVNNVYDVDNKTYLIRFQKPDFKATLLLESGIRIHMTEFEWPKNMMPSGFAMKCRKHLKSRRLVSVKQLGIDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDHEYLILNILRFRTDDADDVKFAVREHYPVDHAKTATSLPNLERLTELITSAPKGDQLKRVLNPHLPYGITLIEHCLIEAGFSGSIKVEQIESKDIEKVLAALQKAEDCMEETENFNAKGYIIQKREKKPSLEPNKSPEEILTYEEFHPFLFSQHSKCPYVEFESFNKAVDEFYSKLESQKIDLKALQQEKQALKKLENVRKDHKFRLEALHQAQEIDKVKGELVEMNLEIVDRAIQVVRSALANQIDWTEIGVIVKEAQAQGDPVASAIKELKLQTNHLTMLLKNPYVISEEEDEEDEEEEKPKVEESKGKKKKKKVKQLQKPLKNQPMLVDVDLSLSAYANAKKYYDHKRHAARKTQKTVEAAEKAFKSAEKKTKQTLKEVQTVTTIQKARKVYWFEKFLWFVSSENYLVIAGRDQQQNEMIVKRYLNPGDIYVHADLHGATSCVIKNPSGDRIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHNQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHRGERKIKVQDEDMETVTSATSELAAEETEPLDAPEGDESSSDDEAHKEEGEEKPENEEHLQDEERDGNGAGDALGMEESSEEEDDVLEEEEEEEPTSNASEILFPDTTIDLSHLQSQRSQLKFALKDNISSLGDSKSQGRRHLSAKERRELKKKKPPSDQDDSDLAKGKDEDHSPQLQLSSNDCKGAGTQQPVKRRQKNKLKKIKEKYKDQDDEDRELIMKLLGSAGSNKEEKPKKTKKGKTNQESAKKQSQKQRRDQKAGQGARIEELIPELEGATLEEHLDDKEEQDQDQNINEEGTALLDSLTGQPHQEDILLFAVPVCAPYTAMAGYKYKVKLTPGTQKKGKAAKTALHNFMQSKESTTREKDLFRSVKDTDLSRNIPGKVKVSAPNLQHLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRA0</accession>
    <name>A0A6I9XRA0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sugar phosphate phosphatase</fullName>
            <ecNumber evidence="13">2.1.1.-</ecNumber>
            <ecNumber evidence="13">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ARMT1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 13">Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate. Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism. Has also been shown to have O-methyltransferase activity that methylates glutamate residues of target proteins to form gamma-glutamyl methyl ester residues. Possibly methylates PCNA, suggesting it is involved in the DNA damage response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 13">
            <text>beta-D-fructose 1-phosphate + H2O = D-fructose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:35603"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>beta-D-fructose 6-phosphate = dihydroxyacetone + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:28002"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="domain">
        <text evidence="13">Subfamily III proteins have a conserved RTxK motif about 40-50 residues from the C-terminus; the threonine may be replaced by serine or cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the damage-control phosphatase family. Sugar phosphate phosphatase III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="13"/>
    <dbReference type="EC" id="3.1.3.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013916286.1">
        <property type="nucleotide sequence ID" value="XM_014060811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRA0"/>
    <dbReference type="GeneID" id="106544520"/>
    <dbReference type="KEGG" id="tsr:106544520"/>
    <dbReference type="CTD" id="79624"/>
    <dbReference type="OrthoDB" id="541375at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051998">
        <property type="term" value="F:protein carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10880:FF:000002">
        <property type="entry name" value="Acidic residue methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.60:FF:000001">
        <property type="entry name" value="protein-glutamate O-methyltransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039763">
        <property type="entry name" value="ARMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260:SF6">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="14">
        <location>
            <begin position="28"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013916286.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="51106" checksum="A3CFC76A2F55EFD7" modified="2020-10-07" version="1">MIKMATPQLVGLPASLTGKIKGSFAYNTIKDRLPLILTRVIDTLHRHKHEFFEEHGEKGIEAEKKAIAYLSKLRNELQTDKPLIALNDNLPDTPLWNQYLEYHKNVSNEPPSWFQSPWLYTECYMYRRIHEAVIQSPPITNYDVFKEEKNHAFFESQQAMIVLSSCLQEMLKNTDGLDEKRLKEEFCNLLQVSLWGNKCDLSISAGADNSQKSDPLQSVEELKKFILVDHMEKLWSLLINKKKNKTPTRLDIVLDNAGFELITDLVLGDFLISSELVTEIHFHGKSFPWYVSDTTKHDLDWTIGQMKASNHKWMARCGVSWAGYLKNGVWIYHDHMFWTLPHEFSSMAQIAPDLHTELQKSSLIIFKGDLNYRKLTGDRKWEFTVPFHQALNNFQPAPLCSLRTLKSDTQVGLKPGQGEKLMNIDPEWMISGKYGIIQFDATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRE4</accession>
    <name>A0A6I9XRE4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Unconventional myosin-XVIIIb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MYO18B</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916652.1">
        <property type="nucleotide sequence ID" value="XM_014061177.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106544790"/>
    <dbReference type="KEGG" id="tsr:106544790"/>
    <dbReference type="CTD" id="84700"/>
    <dbReference type="OrthoDB" id="2505895at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016461">
        <property type="term" value="C:unconventional myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01386">
        <property type="entry name" value="MYSc_Myo18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.270.10">
        <property type="entry name" value="Myosin, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036064">
        <property type="entry name" value="MYSc_Myo18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF8">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-XVIIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="133"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1605"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1909"/>
            <end position="2036"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="955"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1159"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1215"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1367"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1548"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1620"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1671"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1713"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1933"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1981"/>
            <end position="1996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2020"/>
            <end position="2029"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="222"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013916652.1"/>
        </source>
    </evidence>
    <sequence length="2046" mass="228956" checksum="2F27FCA7458B91CF" modified="2020-10-07" version="1">MAISSRLALWEQKIREEDRSPPPTSPPLPFSIIPGGFIKQLVRQKEEESKALRLKEDEASNSQEDVSEDDAWFEAEKLWLLQKDKFTLATELKPDVGTLELPAGTVRARLDADGSIVEVDEEAVQKANPPSLDYTEDLAALVSLNEASVLHVLRQRCQSQLPYIFSGPHLVALRSVPPAVGSSRKAFQGRRDRMPPHVFAVAQRAYWDLLAQRQDQAVVALGRSNAGKTTACQSILEYLVATAGSLDNRVTVEKIQAMFTVLTAFGTVSAGPNRASTRFSMVMALDFSASGHVTAAHLQTMLLERARVAQQPEGEGTFNVFSQMLAGLDLEQRSTLHLHQMAENSCFGIRASLKAEEKRGASAAFAQLQAALGTLGVEAAEQEALWRVLAGIYHLGAAGVCKVGRKQFLKFEWANRAADVLGCEAEELTTAVFKHHLQRILQQVTAGSPSEEPLDDQVPGETRSGPKLSGVECLEGMAAGLYEEVFAAGVMLVNRSFSSSQLSMASLMVVDTPGFLSPRHQQRERAATFEEFCHNYGQERLQGLFYKTTLESEVGRYREENVEVPFLLPELSPAATVALVDQSPSQMLLSSGGPSEDPQGLLWILEEEALVQGSSDSRALDRLCSAFGKEGKAGGEKPGVLRRCEQPLQFEVAHRLGKDPVRYDATGWVGKAKWNLSVQNAIQLLQQSKIGALRKVFLPRAHVPLLCRSVAGWEGHSHQALQRIGCLRKTFSGSWAAVKKRSVCAQIKLQLDALSNLLKRSRIHFLHCLDPGTPPEPQPPTTGTTEAAPGPAWDVPALRAQLSGSLILEALRLHRIGYADRMELVQFRRRFQGLAQPEMKKFTSAYEMTDEKKALEELFRALDLEKKSVALGHTQVFLKAGVLPRLEKQREKLVSQGLVLLQAACRGFLSRQHFRKLKIQSLAARCIQKNLAVYRAVKSWPWWQLMCGVRPLLTISLAEGQLRVKEEELATLQKKLENSDVSRQELRESSELLESKAKQLQLQKRLEEAEKQLGETQQQLQLREIEAKSSGKGHEWQMRLDCAETETAFLRKRIAQLEERLEREQQAKADVEQKLSQAQQLYEEARRAAQQLKRRCQQLTCELEDTRVLMENQQSRNHELEKKQKKFDLQLAQALGEAAFEKSLREKVAQENSGLQFQLGSLRHSLQQKELEKESLAQRVATLSCQMEELGNCASLEAGAPAALQKKLWDLESRTLEQQQELSQQTKALQQLEQLHLRLELEIERTNQIHQKELEDKEEELEDNRKSCQKRLRQLEMQCEQEGDEKQNLLREKQDLEGLVATLCEQIGHRDFDVEKRLRRDLKRTHALLADVQLLLETSGTAEPGPSGSQEEWAKLRSQWEESAARCAEAQESQKTLSLEVENLHSELEAATRNKHLVDEQLYQLQHEKADLLKRFEEDQEDLNELLAKHKALIAQSATDIAQIRELQGQLESLEKEKQALQERLQAAQARLSYLEQATVERSIVSRQEAVICDLESKMEFQSVQIKRFEVLVLRLRDSVIKMGEELEKAKESEARERENAHYYRLRLEEVKADMSELAQRELEASRRRVDLEKQVDELSVARQTLQADLETSLRRIADLQGALEEVQSSEESEAESVQTAQDSLGQRQETAKKDAEESWQPMPWLSAQRKDYGRCGGGPDSPSVGSLKQDYGRPKGEFNRPPKSEAVSPPAWRPLSSPAAAEETAIPFSPPRSRDLSPSAEDRLSRSSSALSEYVEELRRKRLKEPLEEEEEEASSLPIYQTTGAPFLRRCRTLKDKEDLQASLEDLVANPGAELLRCSSLRSISSDCGLRPSLSPALKGASRFGSYDSLVQSAGEPYATGAPTILGGDAAGPLRPRPWRSCLEASVEGSLDLGKEPLLLQSKRLGEIPREAEDPFSWKIPTLSYERKTDADSEDFLPAIRKAQSASGLSRGPKERKDGQRPLSVRFEDQAARPQRTFLSEMKTVLSPRSKAKEDPGNLSDSSDSSTGSFKSADSVKCRPGARRQEGEGCVGRGGSEGPRVHPRSEAEGREEDVTSIMMKYLGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUJ2</accession>
    <name>A0A6I9XUJ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription initiation factor IIA subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GTF2A1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919754.1">
        <property type="nucleotide sequence ID" value="XM_014064279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUJ2"/>
    <dbReference type="GeneID" id="106547198"/>
    <dbReference type="KEGG" id="tsr:106547198"/>
    <dbReference type="CTD" id="2957"/>
    <dbReference type="OrthoDB" id="6275927at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.100:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000002">
        <property type="entry name" value="Transcription initiation factor IIA subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF7">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="68"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="277"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013919754.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="45099" checksum="DDCF6AD94B0A2274" modified="2020-10-07" version="1">MASSTNTNPVPKLYRSVIEDVINDVREVFLDASVDEQVLVELKTLWENKLLQSKAVDGFHSEEQQLLLQVQQQQQHQQHQQQQQQHSHHHHHSQPQQTVQQQSQTQQVLIPATQQTPQSQVMPDSKLIQHMNASTMSAAATAATLALPGGVTPVQQILTSSGQLLQVVRTASGVQYIIQPQQQVVLQQQVIPQMQPGGVQSPVIQQVLAPIPGGISQQTGVIIQPQQLLFTGNKAQVIPTTVAAPTTAQAQISAAGQQQPQQQAAQPQAPLMLQVDGVDDTSSEDEAEKEDYQPAHSQALSVLQVDGAGDTSSEEEEEEEEDYDDDEEEDKEKDGGEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYVFSKAIGDAEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXE7</accession>
    <name>A0A6I9XXE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translational activator GCN1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106548097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920894.1">
        <property type="nucleotide sequence ID" value="XM_014065419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXE7"/>
    <dbReference type="GeneID" id="106548097"/>
    <dbReference type="KEGG" id="tsr:106548097"/>
    <dbReference type="OrthoDB" id="5148094at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25801">
        <property type="entry name" value="HEAT_GCN1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920894.1"/>
        </source>
    </evidence>
    <sequence length="268" mass="29131" checksum="AFE0AC8C0A19B1A0" modified="2020-10-07" version="1">MLQALRFVTQGAGGKVDPAVRKSITTMLLSMLGHEEDATRMTSAGCLAEMCAFLTDEELGGILQNHLLADVSGIDWMVRHGRSLALSVAVNTAASRLCAPKYSNSVHEMIFSNTVADRIPIAVSGIRGMGFLMKHHIEANDGNLPPKLSNLFIKCLQNPSSDIKLIAEKMIWWANKAPLPPLEPPVAKPILKALLDNTKDKNTSVRAYSDQAIVNLLKMRGGEETVQSVSKILDAASLELLSESCRRSLKKLANQADSVEQIDDTILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXS7</accession>
    <name>A0A6I9XXS7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CCDC22</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921039.1">
        <property type="nucleotide sequence ID" value="XM_014065564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXS7"/>
    <dbReference type="GeneID" id="106548224"/>
    <dbReference type="KEGG" id="tsr:106548224"/>
    <dbReference type="CTD" id="28952"/>
    <dbReference type="OrthoDB" id="10266736at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000060">
        <property type="term" value="P:positive regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008530">
        <property type="entry name" value="CCDC22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048348">
        <property type="entry name" value="CCDC22_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048349">
        <property type="entry name" value="CCDC22_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15668:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15668">
        <property type="entry name" value="JM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05667">
        <property type="entry name" value="CCDC22_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21674">
        <property type="entry name" value="CCDC22_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC22 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC22 coiled-coil" evidence="4">
        <location>
            <begin position="68"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921039.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="59125" checksum="C28814C16300CCE1" modified="2020-10-07" version="1">MSARFRIGMSLAQACQDLGYQGEVGYQTFLYSNEPEIRRLLLFLVEKFPRDASEDANQPVGKSATLHRAIADAIKRQLAIPWVPPACRTPGLQLLQGSRLMKPFQAQQLILPQESDPFIGKVSPERREFFVKFLPPVSAQLPQPASLVPSLLELNMTELSAAQEWDSEWKSQGLGSRMTPEEYRQWKHQRLHHRLLDQLRQASRAGPSQQGTTNRELLQLLETFGAGHEQAGALAKGSRFTHTQHLAYKQDAEIPTQVETLPNSRTSEQEFRENQAAELENLEGELSHLIAQIEELEGQMSTLGLTLTQVEGEVRQQQMGVTEQEQILRVKGRTVELLPDAENNMAKLQVLVESSAQRVIQLAAQWEKHRVPLIQEFRELKALHDSKEMESSQRLLEIRELHERIRLAADETKRKDDLYKQLLVEVESLPKDISRSAYTQRILEIVGNICKQKEEITKILHDTKELQKEINSLAGKLDRTFAVTDELVFKVMAGGSEEGRALKGQIEEANVFPLSLSAGCQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZN6</accession>
    <name>A0A6I9XZN6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CAP-Gly domain-containing linker protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106544361</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916083.1">
        <property type="nucleotide sequence ID" value="XM_014060608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZN6"/>
    <dbReference type="GeneID" id="106544361"/>
    <dbReference type="KEGG" id="tsr:106544361"/>
    <dbReference type="OrthoDB" id="5295208at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000002">
        <property type="entry name" value="CAP-Gly domain containing linker protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000001">
        <property type="entry name" value="Putative CAP-Gly domain-containing linker protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF10">
        <property type="entry name" value="CAP-GLY DOMAIN-CONTAINING LINKER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="94"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="267"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="321"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="382"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013916083.1"/>
        </source>
    </evidence>
    <sequence length="530" mass="57264" checksum="5DDE4BB4784E8316" modified="2020-10-07" version="1">MLKPSGLKIPGRAGKHASPVGRPGGTAATASTTSNATKEGSPLHKQASTSSTGASEKLPGSKIAEVGDDFLGDFVMGERVWVNGVKPGIIQYLGETQFAPGQWAGVVLDDPVGKNDGSVGGVRYFECQPLQGIFTRPSKLTRQPTTEGSGSDAHSVESLTAQNLSLHSGTSTPPLSTRVIPLRESVLNNTMKTGNESGSNLSDSGSVKKGDKDFRLGDRVLIQHTNGSEQVPEKQRSHCDVTQRLRKASVNVKQVGGTKTGVVRYVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCPPKFGLFAPIHKVIRIGFPSTSPAKAKKSKRMAMGVSALTHSPSSSSISSVSSVASSVGGRPSRSGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSRICEVEKEIAILKAQHEQYITETEGNLQRARALLDAAQKEKVELLNQLEEEKRKVEDLQFRVEEESITKGDLETQTQLEHARIRELEQSLLFEKTQAAKLLRELEESRLTTVAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZX8</accession>
    <name>A0A6I9XZX8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclin-dependent kinase 5 activator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106547034</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Heterodimer of a catalytic subunit and a regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cyclin-dependent kinase 5 activator family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919536.1">
        <property type="nucleotide sequence ID" value="XM_014064061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZX8"/>
    <dbReference type="GeneID" id="106547034"/>
    <dbReference type="KEGG" id="tsr:106547034"/>
    <dbReference type="OrthoDB" id="7676799at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016533">
        <property type="term" value="C:protein kinase 5 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000025">
        <property type="entry name" value="Cyclin-dependent kinase 5 activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004944">
        <property type="entry name" value="CDK5_activator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23401">
        <property type="entry name" value="CYCLIN DEPENDANT KINASE-5 ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23401:SF1">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 5 ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03261">
        <property type="entry name" value="CDK5_activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009324">
        <property type="entry name" value="Cdk5_activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013919536.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="34229" checksum="846CD2E00CFD0364" modified="2020-10-07" version="1">MGTVLSLSPSGGGGKASLPEEGSTSSLSHYPGGLSSGKGSGQKSDKTLKRPSMFIPALTWKRLVASTKKRGSRAKGAALPGKNFPAGKEVAHLNHENIQKSLSCANLAGYEEGGPPLVPLSTKGLSATTGGPAGSLLKPGGLGGSSTSPRRVIVQASTSELLKCLGEFLCRRCYRLKHLSPTEPILWLRSVDRSLLLQGWQDQAFVTPANVVFVYLLCREMIDGDLVGNEHDLQAALLTCLYLSYSYMGNEISYPLKPFLVEASKDVFWNRCLHIIHAMSAKMLRINADPHYFTQVFADLKNEGNAAAPENFARVLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0J7</accession>
    <name>A0A6I9Y0J7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">GDH/6PGL endoplasmic bifunctional protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">H6PD</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919821.1">
        <property type="nucleotide sequence ID" value="XM_014064346.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919822.1">
        <property type="nucleotide sequence ID" value="XM_014064347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0J7"/>
    <dbReference type="GeneID" id="106547251"/>
    <dbReference type="KEGG" id="tsr:106547251"/>
    <dbReference type="CTD" id="9563"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF7">
        <property type="entry name" value="GDH_6PGL ENDOPLASMIC BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636621" evidence="6">
        <location>
            <begin position="21"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="30"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="218"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="8">
        <location>
            <begin position="561"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013919821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919822.1"/>
        </source>
    </evidence>
    <sequence length="791" mass="90107" checksum="70D0F77DC859938D" modified="2020-10-07" version="1">MLFKETIVVLLLWGIFPTETRESRGHISVILLGATGDLAKKYLWQSLFQLYVDEVTNGHTFTFHGAGQKDPDTGRKLMYDPLKNLACSPEVPPNRCAVLKDQFLKLTEYHQLKGEEDYARLNQGIQTSLAQEELVETGRIFYMSVPPFAYAEIARHINTSCRPPTGAWLRVVLEKPFGRDLLSAQQLAEELTKVFREEEIYRVDHYLGKQAVAHILPFRKQNREKLDPLWNRHHVERVEIVLKETVDVKNRTEFYEQYGVLRDIHQNHLTEILMSLAMELPANLTNSEEVLRRKLEAFSCLQSLEKTSAVIGQYEAYGAHVREELQKGQDYFTKTPTFAGVLVHLDNLRWEGVPFVLTSGKLLDERASYVRVLFKNQVHCVQTESAREAKHSHCKPKQIIFSIGPGELNSPAILVSRNLFKPSMPRADWKEFQEQPQLRLWGQSLSDYYVYVPAAQREAYSFLLSAIYHGKKASFITTENLLASWAFWSPLLDQLSRESPRLYPGGQENGHLLDFKMVGKEVAFIARDPLEVIDAKDKHTADRFQTLLSTFRESRLVTAWADDLIGRLASDVETAALDAIRRRGEFHLALSGGSSPLALFRQLSRHCYGFPWKQTHVWMVDERCVPLADLESNFGHLHQHLLQHIRIPYFNIHPMPVHLNRRLCVEEDGGPQLYANEIAALVANSSFDLIILGMGTDGHTASLFPHSAGLEGDKDIIFAESPTKPHQRMTVTLPLINKARQVSVLVMGKSKHETVSIVSRAGNEPQKWPISGVKPKKGQVSWYIDYESLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0P2</accession>
    <name>A0A6I9Y0P2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ETS translocation variant 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106544696</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916518.1">
        <property type="nucleotide sequence ID" value="XM_014061043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0P2"/>
    <dbReference type="GeneID" id="106544696"/>
    <dbReference type="KEGG" id="tsr:106544696"/>
    <dbReference type="OrthoDB" id="10068174at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09275">
        <property type="entry name" value="RNase_HI_RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000121">
        <property type="entry name" value="ETS translocation variant 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF181">
        <property type="entry name" value="ETS TRANSLOCATION VARIANT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="6">
        <location>
            <begin position="143"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916518.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="32513" checksum="75E6E32A00FA688C" modified="2020-10-07" version="1">MVSGKHILVLTDNVAAHVNRQGSTHSKALRDEALRLGNWAEKHLPSIRAEHISETENHQADWLSRETIDNGEWQLHPLIFHQICKRFGHPTVDLFATPQNAQLPEFVSRFPTAGAMDTDALRCSDIKQEGYREGPPYQRRGSLQLWQFLVALLDDPSNSHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEVLFSLAFPDNQRPTLKAEFEWHISEEDTVPLSHLDENAAYLPDLGGAQPYGKSCTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1J2</accession>
    <name>A0A6I9Y1J2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentatricopeptide repeat-containing protein 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PTCD2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTCD2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910655.1">
        <property type="nucleotide sequence ID" value="XM_014055180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1J2"/>
    <dbReference type="GeneID" id="106540144"/>
    <dbReference type="KEGG" id="tsr:106540144"/>
    <dbReference type="CTD" id="79810"/>
    <dbReference type="OrthoDB" id="6073372at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034913">
        <property type="entry name" value="mS27/PTCD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034629">
        <property type="entry name" value="PTCD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14700">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14700:SF0">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10037">
        <property type="entry name" value="MRP-S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013910655.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="44708" checksum="4F1E9AF6D8469C37" modified="2020-10-07" version="1">MAALVVRTSGRLLRKTAEYPAAGGLVADRVGCWNCFQGAKRYLLTEDVLQLRKFQEKKLENEYKIYGQKDAFFKTVEKKLANNALILKPELINVLYLCQSKNEIELVKRTIYRYHEENSNRAFGEFRFGPIFMRLCYELDLEAVALELMKDQTLNGFFGDYTSFNILMDMLFEKGHYEDALNVLLKMDRENIRFNQDTYLLAFAICYKLNSPEAWTFVNTLLEDKHLHGHELSRRTQYFIVALGLKQNDFLKAQYYFSQLQPTESIVYDNLKILLLAAFGNLKNLVETLEKASKIDTYFVRKPNFCKDVIISAREKLELNPDFIIQFEEIVNKLKVSGQINELTLDDLLCEVPHPKGYKMQLLKETKRSQRTLQPLQSFLLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y265</accession>
    <name>A0A6I9Y265_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Alpha-2,8-sialyltransferase 8E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Sialyltransferase 8E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sialyltransferase St8Sia V</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ST8SIA5</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, GP1c and GT3 from GD1a, GT1b, GM1b and GD3 respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a ganglioside GQ1c (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GP1c (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47592"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:87791"/>
            <dbReference type="ChEBI" id="CHEBI:87792"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:47593"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917133.1">
        <property type="nucleotide sequence ID" value="XM_014061658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y265"/>
    <dbReference type="GeneID" id="106545181"/>
    <dbReference type="KEGG" id="tsr:106545181"/>
    <dbReference type="CTD" id="29906"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000004">
        <property type="entry name" value="alpha-2,8-sialyltransferase 8E isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF4">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE 8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013917133.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="26282" checksum="2E9A389B62B2B7D9" modified="2020-10-07" version="1">MFPKEMPYSRSQFKKCAVVGNGGILKNSGCGQEIDAADFVLRCNLPPISRKYVADVGVKTNVVTVNPSIITERFQRLEKWRRPFYQVLKSYENSSVLLPAFYNTHNTDVSLRVRYVLDDFQSKKAVYFFHPKYLINVSRYWLTQGVRAKRITTGLILVTAALELCEEVHLFGFWGFPMNPSGIFITHHYYDNVKPRPGFHAMPSEMFNFLRLHSRGILRVHTDACGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2T0</accession>
    <name>A0A6I9Y2T0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Palladin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">PALLD</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">Interacts with EPS8. Interacts with LASP1. Interacts with VASP. Interacts with ACTN. Interacts with SORBS2. Interacts with PFN1. Interacts with LPP. Interacts with SPIN90. Interacts with SRC. Interacts with EZR. Interacts with RAI14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
            <location evidence="2">Z line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the myotilin/palladin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920926.1">
        <property type="nucleotide sequence ID" value="XM_014065451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2T0"/>
    <dbReference type="GeneID" id="106548125"/>
    <dbReference type="KEGG" id="tsr:106548125"/>
    <dbReference type="CTD" id="23022"/>
    <dbReference type="OrthoDB" id="6612025at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05892">
        <property type="entry name" value="IgI_Myotilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001560">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000761">
        <property type="entry name" value="palladin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001108">
        <property type="entry name" value="palladin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF10">
        <property type="entry name" value="PALLADIN, CYTOSKELETAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="423"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="557"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="656"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="195"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="519"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="216"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00065150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013920926.1"/>
        </source>
    </evidence>
    <sequence length="803" mass="88580" checksum="F626907288211B7E" modified="2020-10-07" version="1">MGVQNKDNFQHFPPPPSHLEINSVELPPKHSEHSKISHPSSSAFELQLNSVEKKTNGIHPTHGVNGIINGNTKTDKSHPGELLSPTKTPPPVLAKPKLDPLRIQQLQNQIRLEKEADQWCHQKQASFLQNLPLSPTFLPPSSFQELESSHCPSPVQMSVLNSHSSPAMQTSSSFNYARPKQFIAAQNISPASSYVTQASGSSTSSLPSPISPTASQKQYGKTSASPFSQTFAPEEEYPGSPSPPPPPPPVFSPTSTFSVEDSFPLPPPPPPLPASVHSPSCSFSPTSRLSSSNQSPAAFLSSMLPSRPSPMSVNALGLPKSVTPSCVKFPASMAKGNPGYGQIKEKLTYEEKMARRLLGADNAATVFNIQEPEEELAEQEYKVSSFEQRLISEIEFRLERTPVEESDDEVEHGKDVLDSSLTPCFETKLKHYKIFEGMPVTFICRVSGNPNPKIYWFKDGKQISKRNEHYSMQREPDGTCSLHITASTLDDDGNYTVMAANPQGRISCTGRLMVQAVNQRGRSPRTPPNQPHVRRPRSRSRDSGDENEPIQERFFRPHFLQAPGDLIVQEGKLCRMDCKVSGLPTPDINWQLNGRVIRSDNSHKMLVRENGIHSLIIEPVTARDAGIYTCHATNRAGQNTFSLELIVAAKEAHKAPVFIEKLQNTGVAERFPVRLECRVSGVPPPQIFWKKENESLIYNTDRVSMHQDNHGYICLLIQGATKEDAGWYTASAKNEAGIVSCTARLDVYTQWQQQSQTTKPKKVRPSASRYAALSDQGLDIKAAFQPEANPGHLTSGLVESDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2V0</accession>
    <name>A0A6I9Y2V0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U11/U12 small nuclear ribonucleoprotein 35 kDa protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">U1 snRNP-binding protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SNRNP35</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911180.1">
        <property type="nucleotide sequence ID" value="XM_014055705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2V0"/>
    <dbReference type="GeneID" id="106540544"/>
    <dbReference type="KEGG" id="tsr:106540544"/>
    <dbReference type="CTD" id="11066"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12237">
        <property type="entry name" value="RRM_snRNP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000132">
        <property type="entry name" value="Small nuclear ribonucleoprotein U11/U12 subunit 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034146">
        <property type="entry name" value="snRNP35_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF6">
        <property type="entry name" value="U11_U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 35 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="51"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911180.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="29350" checksum="7B00952068FC3C65" modified="2020-10-07" version="1">MNEWVPIAKEYDPLKAGSIDGTDEEPHDRAIWRAMSARYVPNKAVSGDPHLTLFVARLNLQTTEEKLKEVFSRYGDIRKMRLVRDLVTGFSKGYAFIEYKEERALLKAHRDANKLIIDQREIFVDFELERTLKGWIPRRLGGGFGGKKESGQLRFGGRDRPFRKPINLPAMKRDFYGEEKRERAWSRGGSKDWKTGERDYERSRESRRSERYGKSERELEHRGREDRGRGVEGRERERKDRSREAKDGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2V8</accession>
    <name>A0A6I9Y2V8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein KIAA0040 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KIAA0040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911190.1">
        <property type="nucleotide sequence ID" value="XM_014055715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2V8"/>
    <dbReference type="GeneID" id="106540554"/>
    <dbReference type="KEGG" id="tsr:106540554"/>
    <dbReference type="CTD" id="9674"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039964">
        <property type="entry name" value="KIAA0040-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40382">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40382:SF1">
        <property type="entry name" value="RIKEN CDNA 4930523C07 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013911190.1"/>
        </source>
    </evidence>
    <sequence length="107" mass="12217" checksum="64ED5BEC2185EAFC" modified="2020-10-07" version="1">MEQISNFFHSIGSFIFLKHQEGIYNTICLAVLMGLPVLVLLVGIFICCHCCFCGQQQRDKNSHSSSVPIQGKRRHNIMKKKKKKEEDLWISAQPKVFLLAKRPSSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4V4</accession>
    <name>A0A6I9Y4V4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">PPM1K</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013919263.1">
        <property type="nucleotide sequence ID" value="XM_014063788.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919264.1">
        <property type="nucleotide sequence ID" value="XM_014063789.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919265.1">
        <property type="nucleotide sequence ID" value="XM_014063790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4V4"/>
    <dbReference type="GeneID" id="106546819"/>
    <dbReference type="KEGG" id="tsr:106546819"/>
    <dbReference type="CTD" id="152926"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000033">
        <property type="entry name" value="Protein phosphatase 1K, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 11" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="88"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013919263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013919264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013919265.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="40554" checksum="F0902A02B136F2B8" modified="2020-10-07" version="1">MSTAALIGLVRNRGHQLQRALLTSCAILSPKCQISTNDCKASRFDLDGSGCPATWDSFGIWDNRLDEPILLPPSIKYGKLIPKISLSNVGCATHIGKRKENEDRFDYAELTEEVLYFAIFDGHGGVAAADFCNKYMRKYIREFLSEERNMELVLIKAFLEIDKAYMKHAHLSADATLLNTGSTATVALLRDGIELVVASVGDSRALLCRKGKAEKLTIDHTPERKDEKERIRKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKNCGVIAEPETKRVQLHHTSDSFLVLTTDGINFMVNSQEICDFVNQCHDPTEAAHIITEQAMQYGAEDNSTAIVVPFGAWGKYKNSEINFSFSRGFGSSGRWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5A0</accession>
    <name>A0A6I9Y5A0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell cycle control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106547103</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919628.1">
        <property type="nucleotide sequence ID" value="XM_014064153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5A0"/>
    <dbReference type="GeneID" id="106547103"/>
    <dbReference type="KEGG" id="tsr:106547103"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF19">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013919628.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="41037" checksum="AA58B9A98591A362" modified="2020-10-07" version="1">MASGSYNVSRAFSQAPPLQNQPDNTAFTQQRLPAWQPLLTAGTVLPLFFGLGLAFLAIGLSLFFTSEKIKELELDYTGTPGNNCSNCARLTNSSDPRRVRSCQCGLTFKLPAPFPLPVCLYYQLSNYFQNNRRYSISRDDEQLSGVTWALRHPYQECKPYRYNSQGVPIAPCGSVANSLFNDTFELFYQLPNGSLANVTLDKRGIAWWTDTNVKFQNPEPVNNSLCDAFSHTAKPPFWSRTACMLDPSDRNNTGFVNEEFIVWMRTAALPTFRKLYARIRHDNFSKSLPQGTYFLNISYRYPVLGFHGSKKVILSTLSWMGGKNPFLGIAYLVLGAACILSGIVMTVIHLKYRQQDEEDDDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y643</accession>
    <name>A0A6I9Y643_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor BTF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">BTF3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NAC-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920368.1">
        <property type="nucleotide sequence ID" value="XM_014064893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y643"/>
    <dbReference type="GeneID" id="106547661"/>
    <dbReference type="KEGG" id="tsr:106547661"/>
    <dbReference type="CTD" id="689"/>
    <dbReference type="OrthoDB" id="8033832at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd22055">
        <property type="entry name" value="NAC_BTF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000001">
        <property type="entry name" value="Transcription factor BTF3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039370">
        <property type="entry name" value="BTF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10351">
        <property type="entry name" value="TRANSCRIPTION FACTOR BTF3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="4">
        <location>
            <begin position="38"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013920368.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="17685" checksum="F03105DE83CEE97F" modified="2020-10-07" version="1">MKETIMNQEKLAKLQAQVRIGGKGSARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7T5</accession>
    <name>A0A6I9Y7T5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Enhancer of rudimentary homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ERH</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">May have a role in the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the E(R) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915735.1">
        <property type="nucleotide sequence ID" value="XM_014060260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7T5"/>
    <dbReference type="SMR" id="A0A6I9Y7T5"/>
    <dbReference type="GeneID" id="106544104"/>
    <dbReference type="KEGG" id="tsr:106544104"/>
    <dbReference type="CTD" id="2079"/>
    <dbReference type="OrthoDB" id="7887808at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2260.10:FF:000001">
        <property type="entry name" value="Enhancer of rudimentary homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2260.10">
        <property type="entry name" value="Enhancer of rudimentary"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035912">
        <property type="entry name" value="EHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000781">
        <property type="entry name" value="ERH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12373">
        <property type="entry name" value="ENHANCER OF RUDIMENTARY ERH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12373:SF0">
        <property type="entry name" value="ENHANCER OF RUDIMENTARY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01133">
        <property type="entry name" value="ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016393">
        <property type="entry name" value="Enh_rudimentary"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143875">
        <property type="entry name" value="ERH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01290">
        <property type="entry name" value="ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013915735.1"/>
        </source>
    </evidence>
    <sequence length="104" mass="12289" checksum="C6146FF7F63E5279" modified="2020-10-07" version="1">MSHTILLVQPTKRPEGRTYADYESVNECMEGVCKMYEEHLKRMNPNSPSITYDISQLFDFIDDLADLSCLVYRADTQTYQPYNKDWIKEKIYVLLRRQAQQASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9V9</accession>
    <name>A0A6I9Y9V9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">G-protein coupled receptor 56-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106546893</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919355.1">
        <property type="nucleotide sequence ID" value="XM_014063880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9V9"/>
    <dbReference type="GeneID" id="106546893"/>
    <dbReference type="KEGG" id="tsr:106546893"/>
    <dbReference type="OrthoDB" id="8951579at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF318">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013919355.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="11498" checksum="1948C93AC3E42470" modified="2020-10-07" version="1">MLGTMVYEILRLRHREHQWGYAVMLLGLSCVLGIPWGLIFIAFASGTFELVALYLFTIINSLQGFFIFLWYLAKVLQSRRSKSLQCTTSNSLKLQSSNSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAT9</accession>
    <name>A0A6I9YAT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FAS-associated factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FAF1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921005.1">
        <property type="nucleotide sequence ID" value="XM_014065530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAT9"/>
    <dbReference type="GeneID" id="106548195"/>
    <dbReference type="KEGG" id="tsr:106548195"/>
    <dbReference type="CTD" id="11124"/>
    <dbReference type="OrthoDB" id="1920064at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02990">
        <property type="entry name" value="UAS_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14413">
        <property type="entry name" value="UBA_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17129">
        <property type="entry name" value="Ubl1_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17056">
        <property type="entry name" value="Ubl_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01771">
        <property type="entry name" value="UBX_UBXN3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000089">
        <property type="entry name" value="Fas associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000061">
        <property type="entry name" value="Fas associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000045">
        <property type="entry name" value="Putative FAS-associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033043">
        <property type="entry name" value="FAF1-like_UBX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049483">
        <property type="entry name" value="FAF1_2-like_UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044541">
        <property type="entry name" value="FAF1_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF96">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21021">
        <property type="entry name" value="FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="565"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921005.1"/>
        </source>
    </evidence>
    <sequence length="646" mass="73454" checksum="C8D059AE8A00DB78" modified="2020-10-07" version="1">MDREMILADFQACTGIENIDEAITLLEQNNWDLVAAINGVIPQENGILESEYSGESLQRPAYGPASHPSSSSASSSSSAFRPVVPSRQIVERQPRMLDFRVEYRDRNVDVVLEDSSTVGEIKHILENELQIPASKILLKGWKTGDVDDSTILKTLHLPKNNNLYVLTPDLPPPSSSSLPGALQESLNQNFMLIVTHREVQREYNLNFSGSSTIQEVKRNVYDLTSIPVRHQYWEGWPASATDDSITLAASGISYPCHRLTVGRRSSPAQTREQSEEQCTDVHMVSDSDGDDFEDATEFGVDDGEMFGMASSALRKSPMMPENAENEGDALLQFTAEFSSRYGDCHPVFFIGSLEAAFQEAFYGKARDRKLLAVYLHQDESVLTNVFCSQMLCAESIVSYLSQNFITWAWDMTKEANRARFLTMCTRHFGSVVAQTIRTQKTDQFPLFLIIMGKRSSNEVLNVIQGNTTVDELMMRLMAAMEIFSAQQQEDIKDEDEREARENVKREQDEAYRISLEADRAKREAQEREIAEQFRLEQIRKEQEEEREAIRLSLEQSLPPEPKQESTESVSKLRIRTPSGEFFERRFLASSKLQVVFDFVASKGYPWEEFKLLGTFPRRDVTQLDPNKSLLELKLYPQETLFLEAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDN3</accession>
    <name>A0A6I9YDN3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">tRNA pseudouridine 55 synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">PUS10</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:57877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:42533"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013922130.1">
        <property type="nucleotide sequence ID" value="XM_014066655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDN3"/>
    <dbReference type="GeneID" id="106549091"/>
    <dbReference type="KEGG" id="tsr:106549091"/>
    <dbReference type="CTD" id="150962"/>
    <dbReference type="OrthoDB" id="271937at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2050:FF:000001">
        <property type="entry name" value="putative tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2510:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3190:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048742">
        <property type="entry name" value="Pus10_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21237">
        <property type="entry name" value="Pus10_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pus10 N-terminal eukaryotes" evidence="20">
        <location>
            <begin position="113"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Pus10-like C-terminal" evidence="21">
        <location>
            <begin position="293"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013922130.1"/>
        </source>
    </evidence>
    <sequence length="533" mass="60621" checksum="24C5334292002983" modified="2020-10-07" version="1">MVVLTEKDRPVAQMLRNSGTCPRCIFRFCGVGLQTSYRQPHKDLFHELCKFLESKEDENILSCEMANSPCKKIKLENANGIKDQNPNGSHELNYMYSDGNDAPAIENLNPVVCNICLGILQDLCEIDFVKAVCHKINSSEYQFSSFVFSVSFPPQLSVREHAAWLLVKEEMGKQGLSIGKDNFVQLKEAYKWLIHPLFLEELGVPADGKSLFEVSVVFAHPETDEDCCFLASVCPSCFKPAKNKQSVFTRMAVVKALDMIDEEEFHKNYPSPPSSPKTPCTAVEIQCNNGAIFVAGRYNKYSRTLPQTPWIIDGERKMESSVEELILEHLMVAFKADSFNFSSSGREDVDVRTLGKGRPFAVELVNPRKVQLAAVEMKELQKRINSSSDKVQVRDLQLVNREAIVHMKEGEEEKTKSYSALIWTKKVIQKEDIMFLDSMKDLKLDQKTPLRVLHRRPLAVRIRVIHNMQSEYIDEHHFRLHLKTQAGTYVKEFVHGDFGRTKPNIGFLLKTTADILELDVQSVDVDWPPALDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDT6</accession>
    <name>A0A6I9YDT6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Estrogen receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ER-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Estradiol receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Nuclear receptor subfamily 3 group A member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106549259</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear hormone receptor family. NR3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922333.1">
        <property type="nucleotide sequence ID" value="XM_014066858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDT6"/>
    <dbReference type="GeneID" id="106549259"/>
    <dbReference type="KEGG" id="tsr:106549259"/>
    <dbReference type="OrthoDB" id="5799427at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030284">
        <property type="term" value="F:nuclear estrogen receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07171">
        <property type="entry name" value="NR_DBD_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000014">
        <property type="entry name" value="Estrogen receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001292">
        <property type="entry name" value="Estr_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046944">
        <property type="entry name" value="Estr_rcpt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050200">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02159">
        <property type="entry name" value="Oest_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00543">
        <property type="entry name" value="OESTROGENR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0754">Steroid-binding</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="17">
        <location>
            <begin position="226"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013922333.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="38587" checksum="BB3C96989D640CA1" modified="2020-10-07" version="1">MHGCITWLRSTLLPNVSTRFLLPFQVDSRLLIPLQARNLDNATTCSLPVTMTMTLHTKSSGVALLHQIQGNELEPLSRPQLKIPLERPISEMYVDSSKPGGFNYPDGAAYDFSTAAPVYSSTSLSYAPASESFGTSSLGGFHSLNNVPPSPVVFLQSAPQLSPFIHHHSQQVPYYLENEATSFAMREAAPTAFYRPGSETRRHNGRERMTSTNERGNLSMESTKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRIVKHKRQREEHDSRNIGTVAEARSPNLWPSPLMIMEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDW8</accession>
    <name>A0A6I9YDW8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 48-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106549282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922356.1">
        <property type="nucleotide sequence ID" value="XM_014066881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDW8"/>
    <dbReference type="GeneID" id="106549282"/>
    <dbReference type="KEGG" id="tsr:106549282"/>
    <dbReference type="OrthoDB" id="27917at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013922356.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="14279" checksum="F1D5E3C0B7C54AF1" modified="2020-10-07" version="1">MAQCRDLENHHHEKLLETAINTLEKIVKSEFDEEMPEDVRMLFVDKDTIVNAVNASHDIHLLKIDNREDEIITKANNRVYSLIEKIHKDEINRNRSRVLELNHYIEHIRSELDNLDILEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE35</accession>
    <name>A0A6I9YE35_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">Transforming growth factor-beta receptor-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10 11" type="primary">TGFBRAP1</name>
    </gene>
    <organism evidence="7 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922423.1">
        <property type="nucleotide sequence ID" value="XM_014066948.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922424.1">
        <property type="nucleotide sequence ID" value="XM_014066949.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922425.1">
        <property type="nucleotide sequence ID" value="XM_014066950.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922426.1">
        <property type="nucleotide sequence ID" value="XM_014066951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE35"/>
    <dbReference type="GeneID" id="106549327"/>
    <dbReference type="KEGG" id="tsr:106549327"/>
    <dbReference type="CTD" id="9392"/>
    <dbReference type="OrthoDB" id="10258882at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019453">
        <property type="entry name" value="VPS39/TGFA1_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF27">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10367">
        <property type="entry name" value="zf-Vps39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8 9 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="23"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="5">
        <location>
            <begin position="560"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013922423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013922424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013922425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922426.1"/>
        </source>
    </evidence>
    <sequence length="860" mass="97928" checksum="D497717E6D9A0182" modified="2020-10-07" version="1">MSVKAFKLVPAVEWELLTGDRSRIHIECVECCGRNLYIGTNEGSICHFLLEEKNPPSEKTPFSISKQLHKNLSCRKAVGELKAASALNRLLVLCDNTITLMNMMNLEPISTGARIKGALMFTLNENPVNGDPFCVEVCIVSVKRRTIQIYLVYEDKVQILKECCTREQPSAVAVDGYYLCLALTSQYIILNYNTGASQELFPYTGEPVVKRIGREEFLLAAPGGLGMFATVDGISQRAPVRWSEKVIGAALYFPYVIALDEEFITVHSMLDQQQKQTLPFKDGHILQDFEGKVIVATTKGVYFLVPLPLEKQIQDLLGSRRVEEALVLAKGAERNIPKEKFQTMYKRILQQAGFIQFAQLQFLEAKELFRSSQLDVRELISLYPLLLPTSSSFMRSHPPLHEYADLNQLTQGDQGKMIKCKQFLMTYLSEVRSTGVTNGYKEDIDTALLKLYAESNHESLLDLLVSENSCLLADSAAWLEKHKKFFALGLLYHSNGQDATALQLWIQIVNGEIQDSTRTDLYDYIVDFLTSCSDHELVWKYAEWILEHNEEVGVYIFTKRPLEDQEKNSFNPDDVIKCLKKYPVSLVKYLEYLVLEKRIKKEKYHTHLTVLYLNEVLHLKSLGAERQGKLTIMQTKLRSLLQRSDLYRVHFILDKIAGTDLHAECAILYGKLEEHEKALQILVHELKDFSAAEDYCLWNSESKGLPYRRKLFHLLLSTYLNSSTSNHELNVAAVDLLNNHAAEFDAAWVLQLIPASWSIQFLSSFLMGAMRESLHSQRMAEIALSLAKAENLIYKHEKVKLRESPILLSDKKLCQVCQKPFCEPAFVRYPNDTVVHMHCASKRLVNSNTNHHLSSSSGWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE44</accession>
    <name>A0A6I9YE44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">General transcription factor IIE subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Transcription initiation factor IIE subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">GTF2E1</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Tetramer of two alpha and two beta chains. Interacts with TAF6/TAFII80. Interacts with ATF7IP. Interacts with SND1. Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922442.1">
        <property type="nucleotide sequence ID" value="XM_014066967.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922443.1">
        <property type="nucleotide sequence ID" value="XM_014066968.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922444.1">
        <property type="nucleotide sequence ID" value="XM_014066969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE44"/>
    <dbReference type="GeneID" id="106549345"/>
    <dbReference type="KEGG" id="tsr:106549345"/>
    <dbReference type="CTD" id="2960"/>
    <dbReference type="OrthoDB" id="361102at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000087">
        <property type="entry name" value="General transcription factor IIE subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021600">
        <property type="entry name" value="TFIIE_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF8">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11521">
        <property type="entry name" value="TFIIE-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="16">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013922442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013922443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013922444.1"/>
        </source>
    </evidence>
    <sequence length="440" mass="49954" checksum="70B0A4CA1D4B3464" modified="2020-10-07" version="1">MTDPDILTEVPASLKRLAKYVVRGFYGTEHSLTLDILIRNTCVKEEDMLELLKFDRKQLRAILNTLKGDKFIKCRMRVETAPDGKTTRHNFYFINYRLLVNVVKYKLDHMRRRIETDERDSTNRASFRCPICCSTFTDLEANQLFEPNTGIFRCTFCETEVEEDESAMPKKDARTLVARFNEQIEPIYALLRETEDINLAYELLEPEPMEILALKQSKERAANALGNSNLPSGQHREAWTTKGPSYGDMYTQNVIINMEDQEDAHQPANEGKLPRERPVWLRESTVKGAYDPNEVTEGGQDINVLHEDEEGQAALDNNEEVMRALLIHEKKTSSVSTAAMGGIAPLSATNASDSESETSESDEDSLPCAATAPTTLYGLADDNDEDEFEVVTEDPIVMVAGHPFSYSQVNQRPELVSQMTVEEKEQYIAMGQRMFEDMFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF99</accession>
    <name>A0A6I9YF99_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein LOC106549660</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106549660</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922842.1">
        <property type="nucleotide sequence ID" value="XM_014067367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF99"/>
    <dbReference type="GeneID" id="106549660"/>
    <dbReference type="KEGG" id="tsr:106549660"/>
    <dbReference type="OrthoDB" id="9631784at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026234">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334:SF29">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR MEMBER X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02108">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="54"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="419"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013922842.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="51170" checksum="64A2B79C4D7B9766" modified="2020-10-07" version="1">MNSSLGEWNATEDNYEYNNTNDSYVYAEGNTDFVLRWNVWISISFITCCIGLVGNGYIIWLLGFQIKRNCFTTFILNLAVADFGFLTCAFIHHIHPFTNFLECNIFYHVFNYFTRVMLINTNFLLAAISIDRCVAILFPIWHRCSRPNHLSSVVCAFLWISCFLLSGIISVMEIFLKSFHSKRFSEIYNRLMMNSSQEDVDAAEIYYRNNYTNDSYENAREITAFSEWILWKGLTLITCCVGLVGNGYIIWLLGFQIRRNCFTTFILNLAIADFGVSLADGTEWRRHVDQLRRRLPPKTSTATDPTALDTTALQPVTANLWPSANNSQFEAFPQPDVSYPTAASWQPSLVDSQPEFTAVYPHVEAFPQSGLSYPFPCSSQTIPAPPPPGFRSLVPPALYSLHNPTAAYRYCTPFPSTAPPRPGLPQSLHPPSGPVQDGVTHSSTPFGEELPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFF7</accession>
    <name>A0A6I9YFF7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription cofactor vestigial-like protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922921.1">
        <property type="nucleotide sequence ID" value="XM_014067446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFF7"/>
    <dbReference type="GeneID" id="106549728"/>
    <dbReference type="KEGG" id="tsr:106549728"/>
    <dbReference type="OrthoDB" id="10040691at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001223">
        <property type="term" value="F:transcription coactivator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028184">
        <property type="entry name" value="VGLL4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17604">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17604:SF1">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15245">
        <property type="entry name" value="VGLL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922921.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13732" checksum="EB7C0226B5C471A7" modified="2020-10-07" version="1">MLFMKMDLLNYQYLDKMNNNIGILRQYEGEAALRGEPRMQSLPISAAATNHRTGPPPISPCKRRFSMDQGEDDLDCEKEHVPKMSRLFSPHLMKTANGDYRKEHRERSRSPIERAVAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGB1</accession>
    <name>A0A6I9YGB1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TBC1 domain family member 10A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TBC1D10A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923034.1">
        <property type="nucleotide sequence ID" value="XM_014067559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGB1"/>
    <dbReference type="GeneID" id="106549812"/>
    <dbReference type="KEGG" id="tsr:106549812"/>
    <dbReference type="CTD" id="83874"/>
    <dbReference type="OrthoDB" id="159449at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000008">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF23">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013923034.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="30811" checksum="561FADD6A788D6C6" modified="2020-10-07" version="1">MHMPAEQAFWCLVQICEKYLPGYYSEKLEAIQLDGEILFSLLPRVSPPAYKHLSKQKIDPILYMTEWFMCAFSRTLPWSSVLRVWDMFFCEGVKIIFRVGLVLLKYTLGSSEKLRSCQGQYETMEQLRALNPKIMQEAFLVQEVIELPVTERHIEREHLIQLKKWKETHGELQCKCPPRFHGAKAIRDSEPYPHGALEPSPSIILPPGATLSPKGRKSKPRKGAPKGPVTAGHQANGPLPEENGSLLEPDSSPLHRSKESLSSQESEDTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGU4</accession>
    <name>A0A6I9YGU4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SCP2 sterol-binding domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SCP2D1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923401.1">
        <property type="nucleotide sequence ID" value="XM_014067926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGU4"/>
    <dbReference type="GeneID" id="106550124"/>
    <dbReference type="KEGG" id="tsr:106550124"/>
    <dbReference type="CTD" id="140856"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000004">
        <property type="entry name" value="Hydroxysteroid 17-beta dehydrogenase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="47"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923401.1"/>
        </source>
    </evidence>
    <sequence length="155" mass="17277" checksum="C1A736771C64AC14" modified="2020-10-07" version="1">MWKERLKCLQMKINTSEQTAVSDLYEPLMTSRNASTKGPGLQSDLIFEQIGHRIKGVGSQLVKKVNAVFQWDIMNGGKVVAQWTLDLKSGSGQVYPGASHEPANTVFILSEHDFMELALGKIKPQKAFLFGKVKVKGNILLGQKLEMILKEYAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHA2</accession>
    <name>A0A6I9YHA2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAST kinase domain-containing protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923255.1">
        <property type="nucleotide sequence ID" value="XM_014067780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHA2"/>
    <dbReference type="GeneID" id="106550005"/>
    <dbReference type="KEGG" id="tsr:106550005"/>
    <dbReference type="OrthoDB" id="9985850at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000963">
        <property type="term" value="P:mitochondrial RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013579">
        <property type="entry name" value="FAST_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010622">
        <property type="entry name" value="FAST_Leu-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF9">
        <property type="entry name" value="FAST KINASE DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06743">
        <property type="entry name" value="FAST_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08368">
        <property type="entry name" value="FAST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="3">
        <location>
            <begin position="585"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923255.1"/>
        </source>
    </evidence>
    <sequence length="660" mass="76185" checksum="4354ADF9B48857A5" modified="2020-10-07" version="1">MAFMVARSFHIYVSNIQACRQLLTGSRAFHHFCQKMTISYFDSSILEKRRGFPCTFRRNYHVKLKNHTNPVVEAVQHLGEANNDLKSNSKTVGEQIILKELNQWSSYDDIFKFVSSLESLSDTMVAAIFQRLGEVQLQDSLMKSPELLTENDMFKSLCLQLEKDSIHISDSALVNSLNALAKLHVDPCSSLMVSLVSESQQRLDKGQMTIKNLCILGEGLFNLGGPEPAMLEQIMNQIQSTNVEDWKVEEMILVYRTLQLYADRKTNMFQNLLSKMNSFAETEGCQFTPKQTSTVLSALVVLDETQVNNLIIKLCKHASCYIPYFTDDEMINVLEAFIHFRCSDQCFTEALEKHVTKFGFVMHPNTISKIMQYCSTLCILSKPIFNAVAETFVYSADNFTVPQIVEQVVPFGKLNYLPPSAPSFFRQVERFVSTRISEFEPHELLNLLHSCILIQCYPLNFMEKLFSPFFLQELQAKSPRPKIFSQLSQLILTIQLECPYYKKPWLPLDYQVKSFYTRCQSLESKVDLHLFNRVKAGMIDLLGSQKYFVYNVLTPYHYTIDIEIKLNEEGFVLPVNAHDEIYERIALCIDDEKRFCANSHNLLGKESIKQRHLKLIGYVVVQIPFFEFNPLDNKNDVVEYLHKKVFPNFYSFHENKAEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHD7</accession>
    <name>A0A6I9YHD7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 25 member 46-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106550031</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923285.1">
        <property type="nucleotide sequence ID" value="XM_014067810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHD7"/>
    <dbReference type="GeneID" id="106550031"/>
    <dbReference type="KEGG" id="tsr:106550031"/>
    <dbReference type="OrthoDB" id="2403262at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090149">
        <property type="term" value="P:mitochondrial membrane fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039158">
        <property type="entry name" value="SLC25A46"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21252:SF2">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN SLC25A46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21252">
        <property type="entry name" value="TB1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013923285.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="14471" checksum="E49D965C2F18B2DC" modified="2020-10-07" version="1">MHPRRPEGFDGLGYRSAREEPGGFAAKPDLGHWVTTPPDIPGSRNLHWGEKTPQFPAGTPLVAAGLNEDPYLGGAGTNSEQINRFAGFGIGLASLFTENVLAHPCIVLRRQCQVNSLGYCRLLFKYLFCVSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIG8</accession>
    <name>A0A6I9YIG8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">LOW QUALITY PROTEIN: C-type lectin domain family 14 member A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CLEC14A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923996.1">
        <property type="nucleotide sequence ID" value="XM_014068521.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550586"/>
    <dbReference type="KEGG" id="tsr:106550586"/>
    <dbReference type="CTD" id="161198"/>
    <dbReference type="OrthoDB" id="9890094at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990430">
        <property type="term" value="F:extracellular matrix protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789:SF5">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 14 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="154"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="510"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013923996.1"/>
        </source>
    </evidence>
    <sequence length="550" mass="60615" checksum="BDAB9FF0BB8403B9" modified="2020-10-07" version="1">MYTGSDSLYTNYDIKDISREIPKLLAISIRAISLDAIVVARLSIKKKRKGNKFKXSLLAFLRIFKRTVLLDSINSEYLANEFYHKKPEEEENWEHIFFLSKQQAAKFSFRFPLPFCGAESQDARALLRLAHPLVAFLGALGLCRVPRERPRLVPRSRGLLQRPPRPHELLPGSERLRPARGRSEHGQRPHRSRDHPRSAEGTRQRDGRLIVWLGLIKKAHQCTLQDLPLRGFSWVSPGLDEPRANGTAAPPKWLREPNKSCTMQRCAGLQVTLGQAHLNLWGLKDHSCTKGNPGYICKYRYAGTCPALHPASGARGMVYYRLPFQLQSAAVEFSPPGTNLTIRCPGREAHFTCRLSPNGSHWEGTEQQGLCSCPSGYWSPSSGRCAELTDCFSAQGAFRCLCAWRSRLAADEESCIAAARDGAATAKPTVSTPRAAGTFPANGTSRSGQNISFLEPFPTPITNSTTKSSASLNPFNYIFIPIAVVVVILLIMVMASLQVFQKCFKNCSSKGSQPTKDGAVVVEGDPEASATRTNSEPSLGPSKAKPTASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ20</accession>
    <name>A0A6I9YJ20_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PHD finger protein 10-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924353.1">
        <property type="nucleotide sequence ID" value="XM_014068878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ20"/>
    <dbReference type="GeneID" id="106550891"/>
    <dbReference type="KEGG" id="tsr:106550891"/>
    <dbReference type="OrthoDB" id="1903104at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd21085">
        <property type="entry name" value="WH_NTD_PHF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048664">
        <property type="entry name" value="INI1_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21459">
        <property type="entry name" value="INI1_DNA-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWI/SNF Subunit INI1 DNA binding" evidence="1">
        <location>
            <begin position="35"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924353.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13846" checksum="B5D930F7689A4237" modified="2020-10-07" version="1">MGSGDSSRSCETSSQDLSFSYFPAENLIEYKWPPDETGEYYMLQEQVSEYLGVTSFKRKYPDLERRDLSHKEKLYLRELNVITETQCTLGLTALRSDEVIDLMIKEYPAKHAEYSVILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJB1</accession>
    <name>A0A6I9YJB1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium-coupled neutral amino acid transporter 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC38A3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924448.1">
        <property type="nucleotide sequence ID" value="XM_014068973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJB1"/>
    <dbReference type="GeneID" id="106550982"/>
    <dbReference type="KEGG" id="tsr:106550982"/>
    <dbReference type="CTD" id="10991"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015182">
        <property type="term" value="F:L-asparagine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015817">
        <property type="term" value="P:histidine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF22">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="434"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="66"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013924448.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="56254" checksum="8070F49524461874" modified="2020-10-07" version="1">MNSTDIPIQTEMIDIVPNGKHSVLHTSSMGNDSRFDENQVNVTEMEEFLPHGVEKKQTHFTDFEGKTSFGMSVFNLSNAIMGSGILGLAYAMANTGILLFLFLLTTVALLSSYSIHLLLKSSGIVGIRAYEQLGYRAFGTPGKLAAALAITLQNIGAMSSYLYIVKSELPLVIQTFLALEEKTADWYMNGNYLVILVSITIILPLALMKQLGYLGYASGFSLSCMVFFLISVIYKKFQIPCPLPERINNTGNLNYTLISTSSYENEHTTLQATEEDICSSSLFMLKSQTAYTIPIMAFAFVCHPEVLPIYTELKNPTKKKMQCISNISITVMYVMYFLAALFGYLTFYGQVESELLHTYSRVDSLDVLILCVRVAVLIAVTLTVPIVLFPVRRAIQQMLFQDKDFSWIRHIIIAFLLLTSINLLVIFAPSILGIFGLIGATSAPCLIFIFPAIFYIRIVPKDKEPMKSLPKILASLFAVLGVLFMIMSLSFIIIDWTSGGLKSGGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJY7</accession>
    <name>A0A6I9YJY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Urotensin-2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Urotensin II receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106551006</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">High affinity receptor for urotensin-2 and urotensin-2B. The activity of this receptor is mediated by a G-protein that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924476.1">
        <property type="nucleotide sequence ID" value="XM_014069001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJY7"/>
    <dbReference type="GeneID" id="106551006"/>
    <dbReference type="KEGG" id="tsr:106551006"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001604">
        <property type="term" value="F:urotensin II receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097746">
        <property type="term" value="P:blood vessel diameter maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14999">
        <property type="entry name" value="7tmA_UII-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000183">
        <property type="entry name" value="Urotensin-2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000670">
        <property type="entry name" value="Urot_II_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230:SF60">
        <property type="entry name" value="UROTENSIN-2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00647">
        <property type="entry name" value="UROTENSIN2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="530"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="562"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="641"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="729"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="770"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="18">
        <location>
            <begin position="92"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="541"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="338"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="476"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="486"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013924476.1"/>
        </source>
    </evidence>
    <sequence length="851" mass="95076" checksum="808AE45CBD4EF3A9" modified="2020-10-07" version="1">MSRWSKFLSAYSYRLHYQPGKHMGHADTLSHCLLLAQVSDLVLSVASAVFLVDDLELPLSTSDITQATSSNPLLHHVLNAVRWGWTVDSWAPKFLPFLCRCTKLSILQGCLLWGSRVVIPPTLHQLVLRRLLGHPVDTLSKWVEVIPMRSITSAATIHALECLFLTHGLPDILVTDNGPQFASAEFCLYLASLGVRHAPVAPYHPASNGMAERAVRSAKESLARLSHLPWPARLCTYLQAQHSTPCSTTGKSPAEILMGRRLHTTLDWLHPHFAPLSMPQPDGPRTFLVGEEVYAQNYSGEPKWVPAMIQALTGPCSYRVQIGDGRVWRCHIDQLRRHATAQPAGPQAHAAPAAPPNTGLQPQPLQRNQPGYPPPTTTAAVNLSLNSGMRQGAGPPILLCHSNRAPPPSPTSSATTTELRQSQRLRSRPKYLNDYACAIRGEGVLRPLDAASSQVTPCRKLIGSGAAASRRENCGIPIGRPPDSYSVATETETGSPLRSNPNISSNNTWGEAFSASSLEDMVATCTIETILSLMCIIGVTGNVYTLVVMCHYMRSSASMYIYIINLALADLLYLLTIPFIVGTYFIQEWYFGDTGCRILFSLDFLTMHASIFTLMVMSTERYFAVLKPLDTVKRSKSYRKAIALIIWVASLLLTLPMLIMIQLVQGEKNICLPTWSKLSYKIYITILFCTSIVGPGVIIGYLYVRLARTYWVSQTTSLKETKRLPNQKVLYLIFTIVLVFWACFLPFWIWQLLFQYYEPLPMSPKAIRNVNYLTTCLTYSNSCINPFLYTLLTKNYKEYLRNRQRSFHSSRNYFQRRNRFQRISGRSLSTTSQHCTETFMLPPIPGGSNSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKI4</accession>
    <name>A0A6I9YKI4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SMARCD2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMARCD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924549.1">
        <property type="nucleotide sequence ID" value="XM_014069074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKI4"/>
    <dbReference type="GeneID" id="106551066"/>
    <dbReference type="KEGG" id="tsr:106551066"/>
    <dbReference type="CTD" id="6603"/>
    <dbReference type="OrthoDB" id="10263741at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17675">
        <property type="entry name" value="SWIB_BAF60B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.245.10:FF:000001">
        <property type="entry name" value="SWI/SNF-related matrix-associated regulator of chromatin subfamily D member 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030090">
        <property type="entry name" value="SMARCD2_SWIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="4">
        <location>
            <begin position="284"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924549.1"/>
        </source>
    </evidence>
    <sequence length="509" mass="57787" checksum="9BA2DEE37C33C146" modified="2020-10-07" version="1">MAGRGGFPLTPPPPEGPSAAVSVAAGVPSPAIPAPGSGLLRGPSPAGAGTYRPMGHGPQFQRPGMLPGGRMPMAGLQVGSPSGSPYGTASSMRPGMPPSLMDPFRKRLLVPQAQPPPALTQRRGLKRRKMADKILPQRIRELVPESQAYMDLLAFERKLDQTIARKRMEIQEAIKKPLTQKRKLRIYISNTFTPGKEESEGGERIASWELRVEGKLLDDPSKQKRKFSSFFKSLVIELDKELYGPDNHLVEWHRMPTTQETDGFQVKRPGDVNVKCTLLLMLDHQPPQYKLDPRLARLLGVHTQTRASIMQALWLYIKYNKLQDCHEKEYINCNRYFRQIFNCSRMRFSEIPMKLAGLLQHPDPIVINHVISVDPNDQKKTACYDIDVEVDDPLKAQMSNFLASTTNQQEIASLDIKIHETIESINQLKTQRDFMLSFSNNPQDFIQEWIRSQQRDLKIITEVAGNPEEERRADFYQQPWMQEAVGRHIFAKVQQRRQELEQVLGVRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKM0</accession>
    <name>A0A6I9YKM0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sec1 family domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106551186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924701.1">
        <property type="nucleotide sequence ID" value="XM_014069226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKM0"/>
    <dbReference type="GeneID" id="106551186"/>
    <dbReference type="KEGG" id="tsr:106551186"/>
    <dbReference type="OrthoDB" id="9040690at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924701.1"/>
        </source>
    </evidence>
    <sequence length="338" mass="38232" checksum="369B24737FFCC68D" modified="2020-10-07" version="1">MVNMMELTSLHTNETSCNIIAPGCLAQPTEPAVRTLWESLMNLKQKEGLMEVRRHLVEAASRENLPIKMSMGRVTPEQLHSYIQLFKKKFDALENHCGLLQIALAVVQTLKDPQNAKWDNFLAFERLFVQNIGESTLFNALKQLLPIIKSYTNRTADDYTPEELLLLLVYIYSIVGEVKTGKELNEAESQVKEAFVQAICDEPELPPLLQKIVGCESSTKVTFQKATAAVNEIFKSLRDVSRARTHMKQFNSVHILGSHSQQASYKPLVKQVVEEIYNPDRPDPVDIEHMSSGLTDLLKTGFSMFMKVSRPHPSDHPILVIFMFCGVRSVVERTMIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNC4</accession>
    <name>A0A6I9YNC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable imidazolonepropionase</fullName>
            <ecNumber evidence="5">3.5.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Amidohydrolase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106552107</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-imidazolone-5-propanoate + H2O = N-formimidoyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23660"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="3.5.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. HutI family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.7" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013925767.1">
        <property type="nucleotide sequence ID" value="XM_014070292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNC4"/>
    <dbReference type="GeneID" id="106552107"/>
    <dbReference type="KEGG" id="tsr:106552107"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050480">
        <property type="term" value="F:imidazolonepropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000007">
        <property type="entry name" value="Imidazolonepropionase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005920">
        <property type="entry name" value="HutI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01224">
        <property type="entry name" value="hutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752:SF1">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925767.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="31526" checksum="6DF309E1FD732B5C" modified="2020-10-07" version="1">MEQLALRGNASVVIGKNGCIEAVGDARAIREQFSGATFDKIIDCSGKCILPGLVDAHTHPVWAGDRVHEFAMKLAGASYMDIHQAGGGIHFTVEQTQKAREDELLKSFKIRLLRMLRAGTTLVECKSGYGLNLETEIKMLRVIERAKKELDIGISSTYCGAHAIPKGKNATEAADDIINTHLPKLKELDLNGEMHVDNIDVFCEKGVFDLSSTKRILQAGKAIGLQMNFHGDELHPMKSAELGAELGAQAISHLEEISDEGILAMAQAKCAAVLLPTTAYILRLKQPQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNW5</accession>
    <name>A0A6I9YNW5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="12">2.7.7.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA-histidine guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">THG1L</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. Also functions as a guanyl-nucleotide exchange factor/GEF for the MFN1 and MFN2 mitofusins thereby regulating mitochondrial fusion. By regulating both mitochondrial dynamics and bioenergetic function, it contributes to cell survival following oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer. Interacts with MFN1 and MFN2; functions as a guanyl-nucleotide exchange factor/GEF for MFN2 and also probably MFN1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013925846.1">
        <property type="nucleotide sequence ID" value="XM_014070371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNW5"/>
    <dbReference type="GeneID" id="106552162"/>
    <dbReference type="KEGG" id="tsr:106552162"/>
    <dbReference type="CTD" id="54974"/>
    <dbReference type="OrthoDB" id="62560at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3000:FF:000001">
        <property type="entry name" value="tRNA(His) guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028980">
        <property type="entry name" value="tRNAHis_guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12 18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="15">
        <location>
            <begin position="35"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="16">
        <location>
            <begin position="167"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="58"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013925846.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="34906" checksum="FE5DD1F6E71F3BFF" modified="2020-10-07" version="1">MLPGPRLSGAFAAVTLGLRLRFRCYVRAAMAKSKFEYVRDFEAEDKCLPNCWIVVRLDGRCFHRFADQHDFKKPNDDCALNLMNKCAQAVMEEMGEIVIAYGQSDEYSFVFKKDTNLFKRRASKLMTLVVAKFASSYVFYWKDYFKDKSLLYPPGFDGRIVLYPNDQNLKDYLCWRQADCHINNLYNTVFWTLVQKSGLTPVQAQERLKGTLANDKNEILFSEFNINYNKEPEIYRKGTVLIWKKVDEIIKKKITTGETEEKEIEQTRMRNRPIPLHCDIIGNEFWEHNPEILLDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP08</accession>
    <name>A0A6I9YP08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="12">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PIGN</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013925445.1">
        <property type="nucleotide sequence ID" value="XM_014069970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP08"/>
    <dbReference type="GeneID" id="106551814"/>
    <dbReference type="KEGG" id="tsr:106551814"/>
    <dbReference type="CTD" id="23556"/>
    <dbReference type="OrthoDB" id="2748310at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000088">
        <property type="entry name" value="Phosphatidylinositol glycan anchor biosynthesis, class N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000091">
        <property type="entry name" value="Phosphatidylinositol glycan anchor biosynthesis, class N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="484"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="507"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="545"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="594"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="618"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="653"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="723"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="13">
        <location>
            <begin position="433"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925445.1"/>
        </source>
    </evidence>
    <sequence length="804" mass="91400" checksum="ED05874A8328DA8A" modified="2020-10-07" version="1">MNMQFFIVAGILVHCVFLISIFDIYFTSPLVHGMTPHQILMPPPAQRLVLFVADGLRADSLYALDDGGNSNAPFLRNIIASRGSWGVSHTRVPTESRPGHVALIAGFYEDVSAVARGWKENPVEFDSIFNESKYTWSWGSPDILPMFAKGATGDHVYTNCYKAEREDFAAEDATILDTWVFDQVKDFFNLAKNNETLFSKLHEQKIIFFLHLLGLDTNGHAHRPHSREYKNNIRKVDEGVQEIVSLVEDFYGNDGSTAFILTSDHGMTDWGTHGASHPSETLTPLIAWGAGIKYPQAVTSQQYDDTFLKEWKLEMWRRQDVNQADIAPLMASLIGVPFPLNSVGVLPLEYLNNTAHFKAESMLTNAVQILEQFKVKMVQKKKTTLSFLFSPFKSLSESEQIDILRKTRISIQHEKYEEAISLCRKLINLALDGLSYYHTYDRFFLGMSITMGFVGWTFYVILIIIKSDTSMTRDMSKNKVPDRFFQYVFAAIGVLIAFFLFIQSLPWTYYIYCLLPVPVWYAVLKEYPVIHVLVTFLLNVQRTQFSGFLIMYAVGIEILVFSFFYRSTLTFGLIAFSTWPVVIRLWIQAKATVLSWILSCLLLAIFPLMPVIGRESNISLVTAAGLLIILIALSYLVTFFRRSKNKYLHHKDLKIYLFQLLSLGASTYIVNSTHSSLQSKQGLPIINQVISWMTLVSSLIVPLLSPTFLSLRLLSILLSLMSTYLLLSTGFEALFPLVLALLMFVWLDVEQEAIQQSGISFKPQLAALSFSYTTDTPQFRPLHVDDLRRAFFYVSFDLGKQIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRE7</accession>
    <name>A0A6I9YRE7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SURF1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probably involved in the biogenesis of the COX complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Mitochondrion inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SURF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926701.1">
        <property type="nucleotide sequence ID" value="XM_014071226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRE7"/>
    <dbReference type="GeneID" id="106552846"/>
    <dbReference type="KEGG" id="tsr:106552846"/>
    <dbReference type="CTD" id="6834"/>
    <dbReference type="OrthoDB" id="10040024at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427:SF2">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926701.1"/>
        </source>
    </evidence>
    <sequence length="309" mass="35460" checksum="909BC08D8C130FBE" modified="2020-10-07" version="1">MAAWQRLLSDGGLRGALGRLRFSCLNDGRRSFARCCLKDALQQPPKILSWRLYPARNFASAATARSTEENILKWGLLLIPIGSFFLGSWQVRRREWKRKLIAELDSRVTAEPIPLPADFYELNELEYVPVKVRGHFDHTKELYVIPRTPVDPEKEARNAGRIMSNVESGAHVITPFYCTDLGITILVNRGFVPRKKMNPETRLKGQIQDDIELTGLVRLSEPRRAFVPENNIEKNAWFYRDLDAMARVTGAEPIFIDADYRSSVPGGPIGGQTRVTLRNEHMQYILTWYTLCAATSFLWYKRFIQKVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRK0</accession>
    <name>A0A6I9YRK0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc metalloproteinase-disintegrin-like batroxstatin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106552490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926260.1">
        <property type="nucleotide sequence ID" value="XM_014070785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRK0"/>
    <dbReference type="GeneID" id="106552490"/>
    <dbReference type="KEGG" id="tsr:106552490"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF32">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="5">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="122"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013926260.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="14437" checksum="255E85FAB39AE4CF" modified="2020-10-07" version="1">MINFINVVYKALGIHVVLIGLNIWCDGDKIVVSATEDNLFSFSVWRQKNLKHKKNDNTQLLTGVQFNGGSFGYAPLRGMCDPWISVGIVQDHSKDVSLVASTMAHEIGHSVGMEHDANSCTCKGGPCIMAASGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRL8</accession>
    <name>A0A6I9YRL8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Kelch-like protein 41a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106553249</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927193.1">
        <property type="nucleotide sequence ID" value="XM_014071718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRL8"/>
    <dbReference type="GeneID" id="106553249"/>
    <dbReference type="KEGG" id="tsr:106553249"/>
    <dbReference type="OrthoDB" id="6482909at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="40"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="605"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013927193.1"/>
        </source>
    </evidence>
    <sequence length="644" mass="70452" checksum="A829F067F852E921" modified="2020-10-07" version="1">MDPSEEEDDGANDEQPEPTVSLKACFSQGLKHLHHRPELCDATLVAGGKRFPCNRALLASISPYFQAVFTSGFKESRDGEVLLEDMDPSVLQNLLGYLYSGELAFSPGSAEDLFIAASRLQLSPALTLISRFLMEKISPENCLRLYMLGHDHNHPALLQGALRYLGLHFGLLFEHQDFLQLDLRALTCILSSDHLAVASEMEVFRAAQRWVGAEPSKRLPVLKTLLGCVRFPLLSQEEVAQVQTEAELPGQQLEVRWQDLDGAGRLQVSGGLRRGMYQEKIVCIKVPRLRDILSVSEDMDCYMECLNPTSGSRTKLPALQLVALPGCSVLDQRLYISGGKHADGSYSSALHEYNSLADRWAQLPAMSTPRSVHMFLTCKQKLFALSGWNDAGPLASAECFDVAQQLWASIASLPIVLRFSASAAYKNKLYLIGGDADSDDVVYKGLLIYDTHLDTWAQVPLEFSLHGAAAVTVEAGICVIGGFFSRKANQPHPNRRFSQLLPCTPNCFLMQEEGIISREVAIPSLPVPLAFAGATSFRGRIYVIGGVCTSRTHDAIYHWQPGEAAWTQDPENLTAQGMVVRRVLKCVTLKVPIAELRALAQETSAGPVAVGVTEPPLPLEKGGKEHRPPSPDLAPGRPSTGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRS5</accession>
    <name>A0A6I9YRS5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ARIH1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013926649.1">
        <property type="nucleotide sequence ID" value="XM_014071174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRS5"/>
    <dbReference type="GeneID" id="106552809"/>
    <dbReference type="KEGG" id="tsr:106552809"/>
    <dbReference type="CTD" id="25820"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20343">
        <property type="entry name" value="BRcat_RBR_HHARI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20356">
        <property type="entry name" value="Rcat_RBR_HHARI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000002">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="128"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013926649.1"/>
        </source>
    </evidence>
    <sequence length="506" mass="58742" checksum="93C70171CC66CCFF" modified="2020-10-07" version="1">MVYLAPCSGFTGGGLGGSGLSGRGPGGGVGGGGGGGLGHEQEEDYRYEVLTAEQILQHMVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQNSAHLLEQGFLNGILSFSSLCRRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRX9</accession>
    <name>A0A6I9YRX9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pericentrin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106552583</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926375.1">
        <property type="nucleotide sequence ID" value="XM_014070900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRX9"/>
    <dbReference type="GeneID" id="106552583"/>
    <dbReference type="KEGG" id="tsr:106552583"/>
    <dbReference type="OrthoDB" id="2020852at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028745">
        <property type="entry name" value="AKAP9/Pericentrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022165">
        <property type="entry name" value="PKK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44981:SF3">
        <property type="entry name" value="PERICENTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44981">
        <property type="entry name" value="PERICENTRIN-LIKE PROTEIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12474">
        <property type="entry name" value="PKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926375.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="27973" checksum="008232D7F431921B" modified="2020-10-07" version="1">MEGEDPAGVREADGSPRALSKAQEQEQEQSYDIGECYKCQDENKNLHAKLMEEQKQLLEDLQKKVETTFQGELQLAEVPDNLKLEAIRLSSNNICTSQMELMQMNLIKEKETALMELREMLNDKHAQEIAVLQSRYHFELEHVNKQNQKEKEEMVLKHQLDMDKMKKKISLEMEEKHIHILETLKKEWALNVDVSLKNVSEELSVKHQAELNELKQNLTAETEELKKTMETLSLEKREAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS69</accession>
    <name>A0A6I9YS69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dihydrolipoyl dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.4.3.2</ecNumber>
            <ecNumber evidence="4">1.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dihydrolipoamide dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106553094</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="5"/>
    <dbReference type="EC" id="1.8.1.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013927001.1">
        <property type="nucleotide sequence ID" value="XM_014071526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS69"/>
    <dbReference type="GeneID" id="106553094"/>
    <dbReference type="KEGG" id="tsr:106553094"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000025">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="17">
        <location>
            <begin position="3"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="16">
        <location>
            <begin position="225"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013927001.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="36576" checksum="0DFC68678536662A" modified="2020-10-07" version="1">MKDDGSTQVINTKNILIATGSEVTPFQGITIDEDTVVSSTGALSLKQVPDKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGLGIDMEISKNFQRILQKQGLKFKLNTKVTGATKKPDGKIDVAIEATAGGKAEVMTCDVLLVCVGRRPFTNSLGLDQVGIELDNRGRIPVNNRFQTKVPNIYAIGDVIAGPMLAHKAEDEGILCVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGTEYKIGKFPFAANSRAKTNADTDGLVKILSHKTTDRLLGAHILGAGAGEMVNEAALAMEYGASCEDVARVCHAHPTVSEAFREANLAASFGKAINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU84</accession>
    <name>A0A6I9YU84_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc-binding protein A33-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553821</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Neurotoxin that produces dose-dependent hypolocomotion and hyperalgesia in mice. May directly act on the central nervous system, as it is 6500-fold more potent when administered intracerebroventricularly than intraperitoneal.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ohanin/vespryn family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927867.1">
        <property type="nucleotide sequence ID" value="XM_014072392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU84"/>
    <dbReference type="GeneID" id="106553821"/>
    <dbReference type="KEGG" id="tsr:106553821"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12905">
        <property type="entry name" value="SPRY_PRY_A33L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0528">Neurotoxin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0800">Toxin</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="230"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927867.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="50025" checksum="7A22A1DBD4039C72" modified="2020-10-07" version="1">MAGKSQEQTIQEELTCSICYELFRNPVMLECMHHFCKECIEKYWNGCPRIATCPQCRQKCPSRSFHPNFIVSNIAEKVRRSASEEHRRKTKMELQKVLQVYQRKREKLLEMKRRNEENKECLVKTSRKLKSEIQAAFQHLHQILREEEGRILMEMATEEEQYMFRLENASLQLIEEISELKKSMDQMQRRLDNSEISSGLQVESLPVRPAVQSETLTLALKQYKELCDGPLQYIIWRKMLKCISPAPNALTLDPDSAHPSLLLSKDLTSVTERESPQAVPRSPRRFLQSVNVLATASFESGRHYWEVWVGNKTKWELGVASDNVDRAARVRLCPDNGYWTIRLWNDSEYWAAATPWVRLRPQCWLRKVGVLLDCKAKKLTFYNAEEMSVLFIFHQLWASKFYPFFSTGFSHGEKNAEPMRICHPLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUM4</accession>
    <name>A0A6I9YUM4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/threonine-protein kinase ULK2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106553389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927345.1">
        <property type="nucleotide sequence ID" value="XM_014071870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUM4"/>
    <dbReference type="GeneID" id="106553389"/>
    <dbReference type="KEGG" id="tsr:106553389"/>
    <dbReference type="OrthoDB" id="9049120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048671">
        <property type="term" value="P:negative regulation of collateral sprouting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000149">
        <property type="entry name" value="serine/threonine-protein kinase unc-51 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ULK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="9"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927345.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="15256" checksum="809DE2315D8BA2FD" modified="2020-10-07" version="1">MEVVGDFEYSKKDLIGHGAFAVVFKGRHRKKTDWEVAIKSINKKNLSKSQLLLGKEIKILKELQHENIVALYDVQEMPNSVFLVMEYCNGGDLADYLQGNSFLLCLQPFNRRNFLMVGTINPWNDLPPEVVGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV23</accession>
    <name>A0A6I9YV23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras-related and estrogen-regulated growth inhibitor</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RERG</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013928152.1">
        <property type="nucleotide sequence ID" value="XM_014072677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV23"/>
    <dbReference type="GeneID" id="106554082"/>
    <dbReference type="KEGG" id="tsr:106554082"/>
    <dbReference type="CTD" id="85004"/>
    <dbReference type="OrthoDB" id="18798at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04146">
        <property type="entry name" value="RERG_RasL11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000942">
        <property type="entry name" value="Ras-related and estrogen-regulated growth inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013928152.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="22526" checksum="2CDBFDD04D0CBE04" modified="2020-10-07" version="1">MAKSAEVKLAIFGRAGVGKSALVVRFLTKRFIWEYDPTLESTYRHQATIDDEVVSMEILDTAGQEDVIQKEGHVRWGEGFVLVYDITDRGSFEEVLPLKNLLDEVKKPKNVTLILVGNKADLDHSRQVSTEEGEKLATELACAFYECSACTGEGNIMEAFYELCREVRRRKMVQGKTRRRSSTTHVKQAINKMFTKISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV56</accession>
    <name>A0A6I9YV56_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ras-GEF domain-containing family member 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RASGEF1C</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927886.1">
        <property type="nucleotide sequence ID" value="XM_014072411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV56"/>
    <dbReference type="GeneID" id="106553835"/>
    <dbReference type="KEGG" id="tsr:106553835"/>
    <dbReference type="CTD" id="255426"/>
    <dbReference type="OrthoDB" id="20825at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000008">
        <property type="entry name" value="Ras-GEF domain-containing family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF186">
        <property type="entry name" value="RAS-GEF DOMAIN-CONTAINING FAMILY MEMBER 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="4">
        <location>
            <begin position="35"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="205"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013927886.1"/>
        </source>
    </evidence>
    <sequence length="473" mass="54386" checksum="00A48580AF3C7849" modified="2020-10-07" version="1">MPQTLSSASMFTSCGFSPHHLHPSKEDDEGGLIFQDGNLTSASLDALIQHLIPTADYYPEKAYIFTFLLSSRLFIEPHELLSRVCHKCIEQQQLDDPVLDKARIRKFGPKILQLLTEWTETFPYDFQEEKMIGCLKDIIHRIAPCDEAYWKKMNQLLQTLNQKLASLNHGQEGIVKISAAVSDKVVAFKSKPPSIQREMLNVCSDPYTLAQQLTHVELERLTHIGPEEFVQAFGHKDPLDSTKPCFSEQKKTSNLEAYVKWFNRLCYLVATEICMPAKKKQRAQVIEFFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWSKVKTAKFFILEHQMDPTGNFYNYRTALRGAAHRSLTAHSNREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFLELAKQVGEFITWKQVECPFERDDNIIHYLHTAPIFTEDGLYLASYESESPENQTEKERWKNLRSTILGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWC3</accession>
    <name>A0A6I9YWC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyadenylate-binding protein</fullName>
            <shortName evidence="7">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PABPC4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928309.1">
        <property type="nucleotide sequence ID" value="XM_014072834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWC3"/>
    <dbReference type="GeneID" id="106554218"/>
    <dbReference type="KEGG" id="tsr:106554218"/>
    <dbReference type="CTD" id="8761"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000001">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000021">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000042">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000154">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="12"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="100"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="192"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="295"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="10">
        <location>
            <begin position="532"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="431"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013928309.1"/>
        </source>
    </evidence>
    <sequence length="624" mass="69035" checksum="5D44573C7E67254C" modified="2020-10-07" version="1">MNAAAGSSYPMASLYVGDLHPEVTEALLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPGDAERALDTMNFDVIKGKPIRIMWSQRDPSLRKSGVGNVFIKSLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYAFVHFETQDAADRAIEKMNGMLLNDCKVFVGRFKSHKEREAELGVKAKEFTNVYIKNFGDEMDDKRLKDLFSNYGKTLSVKVMTDPAGKSKGFGFVSFEKHEEASKAVEEMNGKDFNGKIVFVGRAQKKVERQAELKRRFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSITSAKVMLEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEERKAHLTNQYLQRIAGMRALPANTIVSQFQPTSGGYFMPAVSQAQNRPTYYAPNQMTQMRPSPRWQPGGRPQGFQGMHNSMRQSGPRPTLRHLAPTNAAQASCGVPGAPQKTGVAPRPPIATQAPRAVPPYRYAAGIHSPHPAVQPLQAPQPAVHVQGQDPLTASMLAAAPPQEQKQMLGERLFPLIQAMHLSLAGKITGMLLEIDNSELLHMLESPESLRSKVEEAVAVLQAHQAKKVAAQKVDIVTATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXA8</accession>
    <name>A0A6I9YXA8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytoplasmic FMR1-interacting protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106554726</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928932.1">
        <property type="nucleotide sequence ID" value="XM_014073457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXA8"/>
    <dbReference type="GeneID" id="106554726"/>
    <dbReference type="KEGG" id="tsr:106554726"/>
    <dbReference type="OrthoDB" id="10265867at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008081">
        <property type="entry name" value="Cytoplasmic_FMR1-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12195">
        <property type="entry name" value="CYTOPLASMIC FMR1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05994">
        <property type="entry name" value="FragX_IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008153">
        <property type="entry name" value="FMR1_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013928932.1"/>
        </source>
    </evidence>
    <sequence length="992" mass="115055" checksum="C3AFD8E85ED32189" modified="2020-10-07" version="1">MLLKVMGFGLYLMDGSVSNIYKLDAKKRINLTKIDKFFKQLQVVPLFGDMQIELARYIKTSAHYEENKSRWTCTSSGSSPQYNICEQMIQIRDDHMRFISELARYSNNEVVTGSGRQEAQKTDAEYRKLFDLSLQGLQLLSQWSAHVMEVYSWKLVHPTDKYSNKDCPDNAEEYERATRYNYTSEEKFALVEVIAMIKGLQVLMGRMESVFNHAIRHTIYAALQDFAQITLREPLRQAIKKKKNVIQSILQAIRKTVCDWEGGREPFNDPALRGEKDPKSGFDVKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIPLLTSNRYETLLKQRHVQLLGRSIDLNRLITQRISAAVYKSMELAIGRFESEDLTSIVELDGLVEINKMTHKLLSRYMTLDSFDAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTILPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYSNYRDFVGPPHFKVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQLKDIVEYAELKTVCFQNLREVGNAILFCLLIEQSLSLEEVCDLLHAAPFQNILPRIHVKEGERLDAKMKRLESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRIFLDDPIWRGPLPSNGVMHVDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFDVLDFCYHLLKVQKHDGKDEVIKNVPLKKMVERIRKFQILNDEIITTLDKYLKSGDGESTPVEHVRCFQPPIHQSLASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXK3</accession>
    <name>A0A6I9YXK3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RFESD</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_013928764.1">
        <property type="nucleotide sequence ID" value="XM_014073289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXK3"/>
    <dbReference type="GeneID" id="106554579"/>
    <dbReference type="KEGG" id="tsr:106554579"/>
    <dbReference type="CTD" id="317671"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000009">
        <property type="entry name" value="Rieske Fe-S domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054716">
        <property type="entry name" value="Sol_Rieske_ferrdox_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF0">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22543">
        <property type="entry name" value="Rieske_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Rieske" evidence="10">
        <location>
            <begin position="14"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013928764.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17595" checksum="BC5031602CEB8062" modified="2020-10-07" version="1">MVKPSKRKEMSSPIYVGREDDIKKLQRTTATVHGRQVVIFYHGGHFYALDCRCYHAGGPLHLGEIEEINGQSCIICPWHKYKITLNTGEGLYQAINPKEPSAVPKWKSKGEKQRTHRVTVDNGNVYVTLSDLMEYKESDYYAEKYKTEKIAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY24</accession>
    <name>A0A6I9YY24_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Integrin alpha-5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106554365</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928490.1">
        <property type="nucleotide sequence ID" value="XM_014073015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY24"/>
    <dbReference type="GeneID" id="106554365"/>
    <dbReference type="KEGG" id="tsr:106554365"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000001">
        <property type="entry name" value="Integrin subunit alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF3">
        <property type="entry name" value="INTEGRIN ALPHA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0401">Integrin</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="13">
        <location>
            <begin position="2"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="14">
        <location>
            <begin position="116"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013928490.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="41525" checksum="A45A9EA94C65C225" modified="2020-10-07" version="1">MNRGEGGAYEAELHVTIPPEADYSGIQRNYGNISSQSCRYDTVNDTNMVICDLGNPMKAGVSLWGGLRFTVPRITDAKPSIQFDFQIHSKNANNSHSEVKSFVLRVETSLRISSFGASMPDAVALPGWAPGRSVQREQDIGPGVHHVYQFVNEGPSAISEGVLDLSCPLRVQGRAVLYITRYTVQANCTSSSPINPSRLQLEPPPTPPSGQEQHYVQKQDGVWNTGNIGGTFPLKCNEDECFQLHCRMGALQRLQSVSLLLYFRIWSKSFQQKENYPQAIQCDAVYHMQKVPYKIQPLELPTIDLKVSTSIHWAKAETSQGVPLWIIILAILIGLLLLALLIYVLYKLGFFKRSLPYGTAMEKAQLKPQAASEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYX9</accession>
    <name>A0A6I9YYX9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium-coupled neutral amino acid transporter 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106555013</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929254.1">
        <property type="nucleotide sequence ID" value="XM_014073779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYX9"/>
    <dbReference type="GeneID" id="106555013"/>
    <dbReference type="KEGG" id="tsr:106555013"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF184">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID SYMPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="69"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013929254.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29052" checksum="F739480008A64ADC" modified="2020-10-07" version="1">MPFKITLELTEMQNMKLPEDDNVSNDSNDFTEVENGQMNSKFIADRESRRSLTNSHLEKKKCDEYSPGTTSLGMSVFNLSNAIMGSGILGLAFALANTGIVLFALLLISVTLLSIYSINLLLACAKETGCMVYEKLGEQVFGTPGKIIVFGSTSLQNTGAMLSYLFIVKNELPAAIKFLMGEGDTFVAWYVDGRVLVVAVTFLIILPLCLLKNLACNSKLEPKKIPLLWDLLYTSVMLLLLLLLLLLLQRQRLELLRLAQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z047</accession>
    <name>A0A6I9Z047_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Plexin-B1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106555000</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929235.1">
        <property type="nucleotide sequence ID" value="XM_014073760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z047"/>
    <dbReference type="GeneID" id="106555000"/>
    <dbReference type="KEGG" id="tsr:106555000"/>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000126">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF36">
        <property type="entry name" value="PLEXIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026990849" evidence="3">
        <location>
            <begin position="21"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="4">
        <location>
            <begin position="6"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013929235.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="43457" checksum="8128EC50B742E282" modified="2020-10-07" version="1">MMPLGTLLLPVLCFLGPTIPQELRYPTFSRNATKFIHFIMDPDSGTLYLGATNFLFQLTPDLVMEEAVSMGPILDSKDCLPPVFKQDCPQALETDNYVKLLLINSLEKELIVCGSVWQGICEKRTLGFIKHVLFQPRTPGDTQYVVANDPNISTVGLVGYSKENVPLLFVGRGYTSRGVGGSIPPITTRNLRAHPGEVPGPDSHSIFSYEETAKLAVGRLSEYSHHFIKSFTHGSSVYFLFYRRDLKSQSREYKTYISRICLDDAHYYSYVELPLVCRTQEKTYSLLQAAYVAQTEVEGQVGDALFVTFSAWQASLGKLSEESALCVYPMEEVDRFITQTRDLCYTKDGKTEDGREVAYIEYDVSSSCVQLSAVSALLLNFYLTRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0X7</accession>
    <name>A0A6I9Z0X7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106556196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine = phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25325"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-methylethanolamine phosphate + S-adenosyl-L-methionine = N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25321"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57781"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25322"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.103" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013930658.1">
        <property type="nucleotide sequence ID" value="XM_014075183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0X7"/>
    <dbReference type="GeneID" id="106556196"/>
    <dbReference type="KEGG" id="tsr:106556196"/>
    <dbReference type="OrthoDB" id="8300214at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000234">
        <property type="term" value="F:phosphoethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="9">
        <location>
            <begin position="204"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930658.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="48079" checksum="02780F1927E02656" modified="2020-10-07" version="1">MHQFWEEHSQNSSLEEMMLDSGAQELTREDTPEILSLLPFLEGQEVLELGAGIGPNFSFDLIFSNWLFMYLSDVELNALVHQMLLWLRPHGHIFFRESCFYQSEFSAVVLLPVEFWELKSTHLGPGKPGYRYRKQNQNQICWLLQKVPRELKNTLGYDTFQKFLDNEQYATRSIRRYEWVFGPTFVSTGGLSSTKDLVRQLDLKSGQHVLDVGCGLGGSDFYMAKEFGVEVLGLDLSSNMVELALERAQKEASSLVQFEISDVTRRLFPEASFDIVYSRDTILHIEDKISLFRKFLSWLKPGGQLLISDYCCGPRPWSIAFTQYVKQRKYNLLTPDEYGQLLEEAGFVQVRALDSTNQMLSSLTQELRDLETSKEKFVQEFSEEEFESMASSWRAKLQRCAAGDQRWGVFFARKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1G0</accession>
    <name>A0A6I9Z1G0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonine aspartase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">TASP1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Intramolecular proteolysis generates 2 subunits, alpha and beta, which reassemble through a non-covalent association to form the fully active enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929675.1">
        <property type="nucleotide sequence ID" value="XM_014074200.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013929676.1">
        <property type="nucleotide sequence ID" value="XM_014074201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1G0"/>
    <dbReference type="GeneID" id="106555366"/>
    <dbReference type="KEGG" id="tsr:106555366"/>
    <dbReference type="CTD" id="55617"/>
    <dbReference type="OrthoDB" id="77601at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04514">
        <property type="entry name" value="Taspase1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000002">
        <property type="entry name" value="Taspase, threonine aspartase, 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037464">
        <property type="entry name" value="Taspase1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF8">
        <property type="entry name" value="THREONINE ASPARTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="8">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013929675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929676.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="45822" checksum="969B6BA41DB456FB" modified="2020-10-07" version="1">MEACSVPQETIALIMEKGISSVEVPPMSAHATAVRMMVKEHEAKPAQKVRRVGFVLVHAGAGYHSESKAKEYKHVCKRACRKAIEKLQAGALVTDAVTAALVELEDSPFTNAGMGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANKLLCEGQKGKLSAGRIPPCFLVADGAFRWAVDHGISACPPGVMATRLSLAAFKRNKRKLELAEKVETNLFQLKKRRQTSEKENESETLDTVGAVVIDQEGNVAAAVSSGGLALKHPGRVGQAALYGCGCWAENMGFQNPYSTAVSTSGCGEHLVRTLLARECSCALQNEDAHQALLETMQNKFISSPFLANEDGVLGGVIVLRSCRCSAEPNTSQDKQSLLVEFLWSHTTESMCVGYMSAQDGKAKTHISRLPHGAVAGQSVAIEGGVCRLESPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2S8</accession>
    <name>A0A6I9Z2S8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox protein Hox-D1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106556473</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Antp homeobox family. Labial subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930942.1">
        <property type="nucleotide sequence ID" value="XM_014075467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2S8"/>
    <dbReference type="GeneID" id="106556473"/>
    <dbReference type="KEGG" id="tsr:106556473"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000113">
        <property type="entry name" value="homeobox protein Hox-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046327">
        <property type="entry name" value="HXA1/B1/D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946">
        <property type="entry name" value="HOMEOBOX PROTEIN ROUGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="204"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="206"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013930942.1"/>
        </source>
    </evidence>
    <sequence length="287" mass="30559" checksum="F6C010DACC56C3CA" modified="2020-10-07" version="1">MNSFLEYLSRGGDSLSLAGKFSSAEPGSVALRSPCALSAGAVDGVYPGPPPPPPVGSLPDFSKRLAPPRQAPRLQPQQLAPCALPSHLFPSAEFNLLRPEAPYAGPEESAPLHYATSIFPGGGGGGGGGSGGAYLQMDYTALAETFQPCPKDALLRPPPPPDFCPKAGGQASVSTFDWMKVKRNAPPKSKPTACGSSSLPSVVRTNFSTKQLTELEKEFHFNKYLTRARRVEIAKSLGLNDTQVKIWFQNRRMKQKKREREGILIPGVSTQVSPSVCSVAPSTKNSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3A5</accession>
    <name>A0A6I9Z3A5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin carboxyl-terminal hydrolase 35-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106555924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930340.1">
        <property type="nucleotide sequence ID" value="XM_014074865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3A5"/>
    <dbReference type="GeneID" id="106555924"/>
    <dbReference type="KEGG" id="tsr:106555924"/>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049407">
        <property type="entry name" value="Usp38-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21246">
        <property type="entry name" value="Usp38-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="444"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930340.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="55616" checksum="88C72B972CD10682" modified="2020-10-07" version="1">MDKILEAILASSYPDHMKQGLVRRIIEALKRTMDTEQCWSMLELSTKLFLLGDTKFKRSVGKEILEVCGLYHQEAFEEFFNAQFLLSLLQEGYGPLGKRSLYVFDYIHLGLPFVMDGPSANDVFSLLRTEVLRKICERPGLKQCVKISKLLIQYPLCVPTGKRQVLFCQQLVQCIGQFHTTSGREEAIMEFLDQVIQVSLLLQKIWKTQVASILPSLKELFAIISTIDDHDPSIALASVVQYVPLELMDGILRNLTNDDSITDLQMLTAIGRMIEWVSWPLGNSLDKWIIALLKNLAAVKKFSILMEVTLSKIERVFSKLLYPILREGALSILRYMLLSFQHSHEAFHLLLPHIPRVIAALQKENSNSASHCLNQLAELIYCMFFCFSGFPDLYEPLVEAIKALPIPNEDRIKHLLGQNAWTSQKNELANLYPRLASKSETGKIGLINLGNTCYMNSILQALFMASDFRRTVLNLAENTSQPLMAKLQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z434</accession>
    <name>A0A6I9Z434_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SH3 and cysteine-rich domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">24b2/STAC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Src homology 3 and cysteine-rich domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">STAC2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a redundant role in promoting the expression of calcium channel CACNA1S at the cell membrane, and thereby contributes to increased channel activity. Slows down the inactivation rate of the calcium channel CACNA1C.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts (via SH3 domains) with CACNA1S. Interacts (via SH3 domains) with CACNA1C. Has much lower affinity for CACNA1C than for CACNA1S.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931417.1">
        <property type="nucleotide sequence ID" value="XM_014075942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z434"/>
    <dbReference type="GeneID" id="106556904"/>
    <dbReference type="KEGG" id="tsr:106556904"/>
    <dbReference type="CTD" id="342667"/>
    <dbReference type="OrthoDB" id="9991832at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20881">
        <property type="entry name" value="C1_Stac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11985">
        <property type="entry name" value="SH3_Stac2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000073">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000022">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039688">
        <property type="entry name" value="STAC1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035509">
        <property type="entry name" value="Stac2_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135:SF5">
        <property type="entry name" value="SH3 AND CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135">
        <property type="entry name" value="STAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16664">
        <property type="entry name" value="STAC2_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 17" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="88"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="272"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="334"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="200"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013931417.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="43550" checksum="74C4A501CC265EB4" modified="2020-10-07" version="1">MASALYVLFSQQQLQRFKRSLSLKTILRSKSVENFFLRSGNEFKLPSDVLLSPPDSVPPPSPPPAPSEVEFSPRVLHKSLGTLKPMKTHSFQEHIFKKHNPCEICHQLIVGNSKQGLRCKTCKASVHLWCSEEVSHQQCLGKTQATSFRRNLSSPLLLHEPQPQPVPAKESPPAAGPSGKVDPVYETLRYGTSLAYMNRSSFSSTSESPTKSLNEKEELVQNTEGSTASGPTAEENMTDNVFSMATEGENPVLEEKNSGQQPSQGSTRKEVSPMYSYVALYKFLPQENNDLPLKPGDRIMLIDDSNEDWWKGKMGDRIGFFPANFVQRVRPGETVWRCFRTFCGNKEQGQLSLKENQICVGVGKTKENEGFIKVTSGKKRGLVPADTLTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z453</accession>
    <name>A0A6I9Z453_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPL13</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930635.1">
        <property type="nucleotide sequence ID" value="XM_014075160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z453"/>
    <dbReference type="GeneID" id="106556177"/>
    <dbReference type="KEGG" id="tsr:106556177"/>
    <dbReference type="CTD" id="6137"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930635.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="24347" checksum="D5A95B09300CD887" modified="2020-10-07" version="1">MAPSRNGMILKPHFHKDWQRRVATWFNQPARKIRRRKARQAKARRIAPRPVSGPVRPVVRCPTVRYHTKVRLGRGFTLEELRMAGIHKKFAQTIGIAVDPRRRNKSTEALQANVQRLKEYRSKLILFPRKSSAPKKGDSTAEELKMATQLTGPVMPIKTVYKKEKARVISEEEKNFKAFATLRMARANARLFGIRAKRAKEAAEQDVEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4N0</accession>
    <name>A0A6I9Z4N0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sclerostin domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SOSTDC1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sclerostin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931607.1">
        <property type="nucleotide sequence ID" value="XM_014076132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4N0"/>
    <dbReference type="GeneID" id="106557027"/>
    <dbReference type="KEGG" id="tsr:106557027"/>
    <dbReference type="CTD" id="25928"/>
    <dbReference type="OrthoDB" id="6624188at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008835">
        <property type="entry name" value="Sclerostin/SOSTDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14903:SF5">
        <property type="entry name" value="SCLEROSTIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14903">
        <property type="entry name" value="SCLEROSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05463">
        <property type="entry name" value="Sclerostin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026884418" description="Sclerostin domain-containing protein 1" evidence="11">
        <location>
            <begin position="24"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="12">
        <location>
            <begin position="71"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931607.1"/>
        </source>
    </evidence>
    <sequence length="202" mass="22602" checksum="4EC92EFB6EA1939F" modified="2020-10-07" version="1">MLAAALHLPSLLLACALLKSCVAFKNDATEILYTHVVKTVPPGSHNNTMNQARHGGRHAGFDRSSRVQVGCRELRSTKYISDGQCTSINPLKELVCAGECLPLPVLPNWIGGGYGSKYWSRRSSQEWRCVNDKTRTQRIQLQCQDGSTRTYKVTVVTACKCKRYTRQHNESSHNFEGATQAKPNQQHKERKKNSKTTKQSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6N4</accession>
    <name>A0A6I9Z6N4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NAD kinase 2, mitochondrial</fullName>
            <ecNumber evidence="4">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Mitochondrial NAD kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">NAD kinase domain-containing protein 1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">NADK2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013931799.1">
        <property type="nucleotide sequence ID" value="XM_014076324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6N4"/>
    <dbReference type="GeneID" id="106557165"/>
    <dbReference type="KEGG" id="tsr:106557165"/>
    <dbReference type="CTD" id="133686"/>
    <dbReference type="OrthoDB" id="185618at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000021">
        <property type="entry name" value="NAD kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13158">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13158:SF5">
        <property type="entry name" value="NAD KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="66"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013931799.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="55831" checksum="E75DBE063B2E7516" modified="2020-10-07" version="1">MTCLPSYLSGGNPGATLRALLKSLRRQPPPRRHLAVPRVRLAEPAASSFAAAATAGLPLRCPSLAGEGSPPSFSSSSPPSFPLPSPSPSRRCLGSGGRSSLSSKAGGAFRPSRVVVVAKTTRYEFEQQRYRYAGLSEEDLKQLLALKGSNYSGLLERHRIHTKNVEHIVNSLRNEKIEVRLVKRRDYGEETVRWADAVIAAGGDGTMLLAASKVLDRLKPVIGINTDPDRSEGHLCLPVRYTHSFPDALQKFYSGEFRWQWRQRIRLYLEGTGINPVPVDLHEQQLSQEQHSRAHVNGRFQDQRSQISEPHLLPVRSLNEVFIGESLSSRVNYKSCKPSFKFSLHRASYYEISVDDGPWEKQKSSGLNICTGTGSKAWSFNINKIANQAVEEILKIAKSYENLKLPLNKELIQKVTNGYNESLLYSPEEPRLFFSIREPISNRVFSSSRQRGFASKVCIRSRCWDACMVVDGGTSFEFNDGAIASILINTEDALRTVLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z792</accession>
    <name>A0A6I9Z792_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PPP2R5C</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931756.1">
        <property type="nucleotide sequence ID" value="XM_014076281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z792"/>
    <dbReference type="GeneID" id="106557134"/>
    <dbReference type="KEGG" id="tsr:106557134"/>
    <dbReference type="CTD" id="5527"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013931756.1"/>
        </source>
    </evidence>
    <sequence length="579" mass="67212" checksum="C3AE851C9378D3F1" modified="2020-10-07" version="1">MPNKNKKEKSQEPPKAGKSGKSSKDVQDNLDIEVSCKKSNTVSPATQLSKIKVTSTQATAKKEKRQSSSKFSITSNRELQKLPALKDVPLAEQEKLFIQKLRQCCVLFDFVSDPLSDLKWKEVKRAALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDSTLTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPTEFVKVMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQFKAEKLKEKLKMKEREEAWVKIENLAKSNPQYSVYSDTSLVSSPVAMETDGPFIEDLQVLTKGVKEEACLVLTEQKRERPIVRRKSELPQDIYTMKALETHCRAEDLLSHDGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-02-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2H4WM86</accession>
    <name>A0A2H4WM86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="9">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106546746</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2017-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Mah S.A."/>
                <person name="Swanson W.J."/>
                <person name="Moy G.W."/>
                <person name="Vacquier V.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Harderian gland</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="9"/>
    <dbReference type="EMBL" id="KY434664">
        <property type="protein sequence ID" value="AUD07754.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919175.1">
        <property type="nucleotide sequence ID" value="XM_014063700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2H4WM86"/>
    <dbReference type="GeneID" id="106546746"/>
    <dbReference type="KEGG" id="tsr:106546746"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000039">
        <property type="entry name" value="Nucleoside diphosphate kinase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="10">
        <location>
            <begin position="5"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="7">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AUD07754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013919175.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17306" checksum="87407F1D46181B4E" modified="2018-02-28" version="1">MASNTERTFIAIKPDGVQRGLVGDIIKRFEQKGFHLVAMKMIHASEDLLKEHYIDLKDRPFYLGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAENEISLWFKPEELVDYKSCAQEWIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0H9</accession>
    <name>A0A6I9X0H9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protocadherin alpha-C1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106537618</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907277.1">
        <property type="nucleotide sequence ID" value="XM_014051802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0H9"/>
    <dbReference type="GeneID" id="106537618"/>
    <dbReference type="KEGG" id="tsr:106537618"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF153">
        <property type="entry name" value="PROTOCADHERIN ALPHA-C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027072568" evidence="14">
        <location>
            <begin position="18"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="680"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="15"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="122"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="231"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="338"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="443"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="577"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013907277.1"/>
        </source>
    </evidence>
    <sequence length="847" mass="93361" checksum="1DC131901736A0A1" modified="2020-10-07" version="1">MRVYLMVCCLIFYTVRGQVEYSIAEETECGAPVGNLAQDLGIDAARLPSRKFRMITSSSKQYFNLNASTGVLSVNEPIDREEICARQPTCSLNYELVLETPLELYKLQFKILDVNDHTPTFLLEEYHVNVAEFLAPGVRFTLPVAQDLDEGINGVQSYVLSPNQHFRLEMQTRGDGSRYPELVLEKPLDREQQVTHRLTITAKDGGSPQRSGDTQVVVTVLDTNDNAPIFEHSVYRASVLENSPRGVLVTRVQATDLDEGQNGEVFYSFSNSTPPELQKMFSINPQNGEIKVNGALNVQKPLLEMFIEAKDKGVFGMSTVAKLLVEITDVNNNAPEITLTSLSSPIPEDSLLGTVVALLSITDEDPGENGKIICKIPVDIPFQLKSSFDNYYSLITSGLLDREQVSEYNITVTASDLGSPPIATQKALWVQIADVNDNPPKFLNQMREIYIAENNKPGASVFQVSASDPDLRENGRLSYFLRDIDIQGSSLSRYLAINADNGIIYAKDTFDFEQLQNFHFQVEAKDNGFPILTATFIVSVTILDQNDNAPVILYPDVRNASVAVEIVPRLADANYFVTKVVAHDPDSGQNAWLSYHLLHSSDASLFQVLPNSGELRTSRSMRSSDPIKHKVVILVTDSGDPSLSSTVSLGILLADTLPQALPDFDDSLETHGQQLSSLNFYLIIALGCLSLVFFGFIIFLFAVRLFHCRTNPSCNCCCPMDEYEKYRYNVRMLPSSHFTPEMVEIAGMGKLTHTYLYRAALGVGTSNNNSMSNRDAGSLSKGAGLLQVPASCIQVQKVKSDHFNAILVVAAVFLPDANLPVGLFAVLQGHLIFSDGVQEADIWLGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2S6</accession>
    <name>A0A6I9X2S6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NHL repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NHLRC3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909579.1">
        <property type="nucleotide sequence ID" value="XM_014054104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2S6"/>
    <dbReference type="GeneID" id="106539328"/>
    <dbReference type="KEGG" id="tsr:106539328"/>
    <dbReference type="CTD" id="387921"/>
    <dbReference type="OrthoDB" id="10044505at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000043">
        <property type="entry name" value="NHL repeat containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10680">
        <property type="entry name" value="PEPTIDYL-GLYCINE ALPHA-AMIDATING MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10680:SF14">
        <property type="entry name" value="PEPTIDYL-GLYCINE ALPHA-AMIDATING MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="5">
        <location>
            <begin position="213"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909579.1"/>
        </source>
    </evidence>
    <sequence length="348" mass="38539" checksum="29F9B76CE155BD57" modified="2020-10-07" version="1">MGAHDHSRVRLFAGLGGAGLFLLFIVLYRVGSELEVLNQWATYSLWRIQKPLYKLDLEWPKFSEEFTGQTFCVAVDHLQGLVYVGQRGNDDVPKVSVFSEEGYLVQTWKTATIEMPHGIFALNTPNGSSVWITDVGNGKYGHTVKQYSPFGELLRVIGTPGTAGSDTDPLQFDQPAEVFVESTGNIYIVDGDGGLNNRLLKLTQDLKILWLRGENGTAPTQFRIPHSVTVDSVGRVWVADRANWRIQVFAKSTGEWLGSWDKCFTEDGPYSVRFTPDQQYIIVAHLNVNKISILAAPPVGLIGDCIVVDTIQLAREVKPHLLDVSLSTGAIYVAEIGAEQVQKYVPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2U5</accession>
    <name>A0A6I9X2U5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NF-kappa-B inhibitor epsilon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NFKBIE</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909599.1">
        <property type="nucleotide sequence ID" value="XM_014054124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2U5"/>
    <dbReference type="GeneID" id="106539340"/>
    <dbReference type="KEGG" id="tsr:106539340"/>
    <dbReference type="CTD" id="4794"/>
    <dbReference type="OrthoDB" id="10254947at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
        <property type="term" value="P:cellular response to tumor necrosis factor"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="NF-kappa-B_inhibitor"/>
    </dbReference>
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        <property type="entry name" value="NF-KAPPA-B INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680:SF5">
        <property type="entry name" value="NFKB INHIBITOR EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="160"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
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        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
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        <location>
            <begin position="272"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="305"/>
            <end position="337"/>
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    </feature>
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        <location>
            <begin position="33"/>
            <end position="81"/>
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        <location>
            <begin position="98"/>
            <end position="125"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013909599.1"/>
        </source>
    </evidence>
    <sequence length="375" mass="41079" checksum="73CA29B4BDDB56A5" modified="2020-10-07" version="1">MSEIQSYGEKKEMLLEGGGGAYLEEGQCDSGIDFIRSLSGQPGEAEPRSPATAGRIPLDKIPGTPSPGKEEPADERLDSSYGSSSIVESLTGLQGEPNLEKEEEEEQEERLRQQLLKTLDFLSEDGDTLLHLAIIHCVPSIASFCIAQMPVEVLEFQNDLFQTPLHLSVYLEQFEVVEALILKGVNTALQDRNGNTALHLACERQSLECVELLLLLKKPVSEMQTRKTVQDLQLQNWQGLTCLHISTLKGNLQLMELLVQNGANINVQDGTSGKTPLHLAVENHDEMAVKHLLRWGAQVDSQMYNGCTPLHLAVGRNDAAIAAILCHSGADTLLRNMENETAQDLADGNDDILTLLPFDDLKISGKPVMCSSRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X406</accession>
    <name>A0A6I9X406_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 41 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106538591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908592.1">
        <property type="nucleotide sequence ID" value="XM_014053117.1"/>
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    <dbReference type="GeneID" id="106538591"/>
    <dbReference type="KEGG" id="tsr:106538591"/>
    <dbReference type="OrthoDB" id="244107at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="2">
        <location>
            <begin position="30"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908592.1"/>
        </source>
    </evidence>
    <sequence length="160" mass="18590" checksum="FE6AABFE0D985DB2" modified="2020-10-07" version="1">MAETAEKEPMSDEETTDESEEDISEEEPKLKYERLSNGVGEIIQKDVASCMTIHEKFLALGTHYGKVYLLDVQGNITQKFDIVSTNYLPYDYCNLKYCVQNNITFILNLFFCETMKLYEEMLVMVKSFYENVTKNHKEMMGLFKDKDVSVEDPQQMVGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4N9</accession>
    <name>A0A6I9X4N9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cohesin subunit SA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">SCC3 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Stromal antigen</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106539932</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the cohesin complex which is composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SCC3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910334.1">
        <property type="nucleotide sequence ID" value="XM_014054859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4N9"/>
    <dbReference type="GeneID" id="106539932"/>
    <dbReference type="KEGG" id="tsr:106539932"/>
    <dbReference type="OrthoDB" id="498590at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF2">
        <property type="entry name" value="COHESIN SUBUNIT SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="5">
        <location>
            <begin position="183"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="120"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1045"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013910334.1"/>
        </source>
    </evidence>
    <sequence length="1066" mass="123075" checksum="795989BB320C1246" modified="2020-10-07" version="1">MFQSLQNCEVMHKMTEKFDEETGLQYKRFMAYPWILTVTWPVDMGNEDYPLIKPGSYWKKFRTNFCEFTAVLVQQCQCSILYDSYLMDTIISLLTGLADSMVRAFRHTSTLAAMKLLTALVNLNLNLEVSKDNLERLYEVEKKRIVGKKINSRLDQLERKKREYEQKSLQVENMMNAIFKGTFLPRYRDIIPEIRAICIEEMGIWMIIYPDTFLNDSYLKYLGWMLYDKHPEVRLKCLQALQGIYSQKEVVCKMDLFTSRFKDRIVSMALDRDHEVTVQAMKLLMVISQNCDDALSSEDCENLYQFVYSTHRPLAVAAGELLYKRLIIQEIETESFTKTNENQELITSQLKTLIVFFLESELHNHVTYLIDSLWDWAVSLLKDWECMTALLLVDAREYERALSDVQENALIEIMLATVRQAVEGPPTGRGGIRKILSTKEKKIQIEDCAKITEHFIVVLPRLLAKYSLDTEKVTNLLQISQYFDLEQYSTGSFNKNLDALLRELKAIVMIHSNTNILETCSRVYSILSREELTIHNQVAFARAQLMNELVEKLDQLLGIFWHKEDGVSAEEEGIQHLASSLRQIAAFHNAHDLTKWNLYDKIYKLLVSELEQRPLPVQILLPSLQCAYFALLWQLPSMAESPSSKENIFLLRTHLRQYCNICTSYLSHYNRDLREKAFMLLCDLLLILSHQDSSNNDAAGLLEYLPSTSLQTKMLLFIRDHVFGEEEEETKDMRESNVKVKENQKLDALHLKRCLLAAYCKLIICNVVEITAAAEIYKHYMKAYNDFGDIIKETLSRTRHNNKIQSARALILCLQQLFQKHVESQGDGSNSSNNLDSSTTSFNNIKELARRFSLTFGWDQVKSRESVAMIHKEGIEFAFQGAAGMEGKCLPPNLNFLIVLSEFSNKLLKPDKRLVYSYLLRYVPETALCKEDTWYSLAWYRTSLLASDDDENSAISSSQEWPSVSITRTSSFKRKLSEGSWSKISHPDRAHKISETHYPSCLSSLGMRNRKRLKSHDSYHKEYSLPLGSATPKRKHSTQAPVKEEDLEEASLEEADIDVVGADQDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X628</accession>
    <name>A0A6I9X628_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Gamma-aminobutyric acid receptor subunit rho-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">GABA(A) receptor subunit rho-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC106539117</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Three rho subunits (rho-1/GBRR1, rho-2/GBRR2 and rho-3/GBRR3) coassemble either to form functional homopentamers or heteropentamers. Rho-1/GBRR1 subunits can also associate with alpha-1/GBRA1 subunits to form a functional GABAAR. Interacts with SQSTM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRR1 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909277.1">
        <property type="nucleotide sequence ID" value="XM_014053802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X628"/>
    <dbReference type="GeneID" id="106539117"/>
    <dbReference type="KEGG" id="tsr:106539117"/>
    <dbReference type="OrthoDB" id="442503at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19005">
        <property type="entry name" value="LGIC_ECD_GABAAR_rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19059">
        <property type="entry name" value="LGIC_TM_GABAAR_rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000015">
        <property type="entry name" value="gamma-aminobutyric acid receptor subunit rho-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000005">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit rho-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="26" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="23">
        <location>
            <begin position="2"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="24">
        <location>
            <begin position="201"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013909277.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="45236" checksum="F92308BA110B21EF" modified="2020-10-07" version="1">MRPGFGGPSIPVGVDVQVESLDSISEVDMDFTMTLYLRHYWKDERLSFPSTNNQSMTFDGRLVKKIWVPDMFFVHSKRSFIHDTTTDNVMLRVQPDGQVLYSLRVTVTAMCNMDFSRFPLDTQTCSLEIESYAYTEDDLMLYWKNGNESLKTDDRISLSQFLIQEFHTTTKLAFYSSTGWYNRLFINFTLRRHIFFFLLQTYFPATLMVMLSWVSFWIDRRAVPARVPLGITTVLTMSTIITGVNASMPRVSYIKAVDIYLWVSFVFVFLSVLEYAAVNYLTTVQERKERKQCEKFSGASAIPQPKQMTGMEGSYSDGEVNELGQYVSENGGKEDRMMVQLGLGSEGNSNRRKIQRYSSMRIDTHAIDKYSRIIFPGSYILFNLIYWSIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7Y5</accession>
    <name>A0A6I9X7Y5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CSNK1E</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013910416.1">
        <property type="nucleotide sequence ID" value="XM_014054941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7Y5"/>
    <dbReference type="GeneID" id="106539990"/>
    <dbReference type="KEGG" id="tsr:106539990"/>
    <dbReference type="CTD" id="1454"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000194">
        <property type="entry name" value="Casein kinase I isoform delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="301"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="350"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013910416.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="47499" checksum="D46A11F13F2601C7" modified="2020-10-07" version="1">MELRVGNKYRLGRKIGSGSFGDIYLGANIATGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDMDRERREHEREERMGQLRGSATRALPPGPPAGATANRLRNVAEPMASTPTSRIQQSGNTSPRAISRVDRERKVSMRLHRGAPANISSSDLTGRQEVSRISASQTSVPFDHLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8D1</accession>
    <name>A0A6I9X8D1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cysteine-rich venom protein kaouthin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106540976</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911769.1">
        <property type="nucleotide sequence ID" value="XM_014056294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8D1"/>
    <dbReference type="GeneID" id="106540976"/>
    <dbReference type="KEGG" id="tsr:106540976"/>
    <dbReference type="OrthoDB" id="737510at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000005">
        <property type="entry name" value="Cysteine-rich secretory protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="SCP" evidence="5">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911769.1"/>
        </source>
    </evidence>
    <sequence length="152" mass="16944" checksum="551ADC15D01D9D3F" modified="2020-10-07" version="1">EWNSNAAQNAKRWADRCTFAHSPGHLRTVGRLGCGENLFMSSHPYSWTRVIQSWYDEYKNFRYGVGANPPGSVIGHFTQIVWYNSYLVGCAAARCSSSRFKYFYVCQYCPAGNIIGSIATPYKSGPPCGDCPSACVNGLCMVDEPMQLNQEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAT8</accession>
    <name>A0A6I9XAT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Versican core protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Chondroitin sulfate proteoglycan core protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Large fibroblast proteoglycan</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">PG-M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">VCAN</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="33">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Interphotoreceptor matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912719.1">
        <property type="nucleotide sequence ID" value="XM_014057244.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541725"/>
    <dbReference type="KEGG" id="tsr:106541725"/>
    <dbReference type="CTD" id="1462"/>
    <dbReference type="OrthoDB" id="9905227at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033165">
        <property type="term" value="C:interphotoreceptor matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03588">
        <property type="entry name" value="CLECT_CSPGs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05901">
        <property type="entry name" value="Ig_Versican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03517">
        <property type="entry name" value="Link_domain_CSPGs_modules_1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03520">
        <property type="entry name" value="Link_domain_CSPGs_modules_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000011">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000195">
        <property type="entry name" value="versican core protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000361">
        <property type="entry name" value="versican core protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033987">
        <property type="entry name" value="CSPG_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF6">
        <property type="entry name" value="VERSICAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 22" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0373">Hyaluronic acid</keyword>
    <keyword evidence="16" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="11" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 26" id="KW-0732">Signal</keyword>
    <keyword evidence="6 23" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026900806" description="Versican core protein" evidence="26">
        <location>
            <begin position="18"/>
            <end position="3101"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="29">
        <location>
            <begin position="30"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="31">
        <location>
            <begin position="148"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="31">
        <location>
            <begin position="249"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="27">
        <location>
            <begin position="2793"/>
            <end position="2829"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="27">
        <location>
            <begin position="2831"/>
            <end position="2867"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="28">
        <location>
            <begin position="2880"/>
            <end position="2994"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="30">
        <location>
            <begin position="2998"/>
            <end position="3058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1029"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="2017"/>
            <end position="2044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="2191"/>
            <end position="2232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="2542"/>
            <end position="2564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="2191"/>
            <end position="2201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="2203"/>
            <end position="2212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2222"/>
            <end position="2232"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="2819"/>
            <end position="2828"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="2857"/>
            <end position="2866"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="3000"/>
            <end position="3043"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="3029"/>
            <end position="3056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00044266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_013912719.1"/>
        </source>
    </evidence>
    <sequence length="3101" mass="342053" checksum="AFAA08BEC47C87C2" modified="2020-10-07" version="1">MFKHFLWIYWTLSITCALHKVKVEKSPPVKGSLSGRATLPCFFSTLPTLPPSYHNVSEFLRIKWSKIEQDIGGKDLKETTVLVAQNGNIKIGQGYKGRVSVPSHPEDIGDASLTMVKLRASDAGVYRCDVMYGIEDTQDIVSLAVDGVVFHYRASSSRYTLNFKQAQEICLKNGAVIANPEQLKAAYEDGFEQCDAGWLADQSVRYPIRQPRVGCYGDMMGKEGVRSYGYRLSNETYDVYCYVGNINGDVFHVTSPNKVTFEEAQTLCEEQGAVLASVGDLHAAWRNGFDQCDYGWLADGSVRYPASVARIQCGGGLLGVRTLYRFENQTGFPYPDSKFDAYCFKRKENVTDFSMVELNIPLESGSAKSLTELNVIPPKTTLHPYVSSLTSEMVMVKQEKKEPRTINLSTLLPQTVTDVSDLSKMTQTETVLSDLVEITPTQDISDLHEASSESSVEQIEVAPIRISVDSVARNNLSVPLETKEETHIGIQPSKIPVEAKSDNKFTTVVIPKELLTSIYESVEEDKVILPDKKLITSSTISKVDKIPSSTTILDEKATDTPDSVATSFESKTMSTPAYSTGFEIPVLTPSDRISVVHSTDSVEKEEHTVASVVSGKMKDTVTFIPILRASDVSSGSEVSIGEGSMFGPESATKHMPFATNVNEVEGFFTSYGPEMSSHHHLITPTPDLQQETIETDAKYDWTNEENTTEQQPIFSISKEDYPEMVTEGIEIKQSFIVTDTTKTATPVAISEKDYSSSLISDTIENEISKGSGIVTTHTALTETITEVVPVIQQESVTRDLITQTLVTDPASPTVVPELYSLPEHDVAAEGSAYEINYTTTDTSPGIKIRDYDTVTQSELSPGIIDPKLIATGVPPIEPTTKSEHRSSLDKLVVPISREEAAPVTKKFDVGEVDTSTHSPEGSGIKEEDIGMEPISFSVSATSKPMVVTGIATTVGTAVDKIPPTSASKLLIIKTQPPLIDGGPDEDTNKDVLIIDESISPIKTTTDDDFTGKMVEPEIDTEYFTSSTITAVSQPTGPPTEVLESQEEPPATSEVATGLENESNIHVYVVAIPQNDTDSLPGLWDIFGYHIHHELDEPPLDESATEEPCTESPEDVADDILIVDPFFPEIYPLEEDESEEVDCENTTAITTPPPLQFINGKQQVTPAPKDIKAEEARSDQILAHSKNVNFLHINETNMVSDNEFLATIQPSESTGDTAATQKLRAEYDIVEEVFSGDPETTHKIHEIIYLHGQSLSDNNEKLIQSETEHLEDLTAQPESISSASPHTTNMELKEDPEQLILTASTQGWLPHSTTIYLDTGDDIVAQHDTSVPQENYNTNVRHNVLNSYINLLTTTSSTNLLNISEGSGAVQEDGYKSTSALIKPGIQEKHPSQEIFSDDGNPIPPKPSASQSPASLYKETMFIAEHSLLGSLTTDSITEIILSTTKSQINMEGDKEDSTSSMSLNMDNSTAFSTENLFNSNEYLTDNIRVVSEGSIAVTQTISPLKTKEITENRLSINEESGDGELWRKPNKEILLGGPTSQVVSIDSQFLDAGFGGIDSMTQATTQTSFPMRSEYESSKGKEIGSISSEPPLNHAIHTNHTMDVLEAELYGDATDTRIESQVSKNETYSQRASDKSEASVLLPFNDKSVVETNEEVITTITIQEQTNLFETSSMANISPAISVGMKLIPELIETDRTAQHSSSTAQDSIVVTNNITLQKGSSEFIVNEGMESLQLEDRKNISPTTFAKTIDLVEIGSGEESVNDTSSMKLQNYTSKEKEINSKLPLIEPGSGDVDSITYSSTKKPVSSEFLVQESKNQIKNKSEESSMVFQHPLVTTLNISSTTIVTLMNQNITGAFVNKSKMAQEMPNVLGTKASDKTSHLEGEIYYEEKRKIIETESVTQQSEISGTSKFNTHAKELQFDINSVTESLQKYPPSYDKTASTLLRNVEKEEWLEEKITPAKDSHTLPPPSTIYRPITTATHVPFLSKQDSKNELFLVHTTAPGNSATLKESSNALKLDTPYPKSSEELFSEEQHEEFERHNDDSKYEFKYPSKLEDNLSSASDETFPVLSIVHHETSTINWFISEQDQNKEKSYQGEQNVLAATDRTSQGKAWVFVKHENKTSASKLFIEPPSSGTIETPHATLLSKVSGAGNHDGSGEGSGWQDVIEKKSESFTQLPNWELPLLTRPPHYSDIENKGDTMDVSQKGPNTQTEKHEKTATSDSDISESFSYESSTNNIKDYEDLIPVAGFKKNYSEDISSFIDIIGTGHGVILDETTIIDVDNLKSNSNIEDIIDHTTKRPGRHSTYNIYTSAIKIQNTTPEMLIDDKITDNQGSSVGNKFIHSESILSDHTPTLEVGSGDIASFTTESEISQDPLKTSLLLPTLPLLVSSTISSPITSNENTRDVGDGIERTESEEMIQSKSEQPTPSDNQVVADESEITTMSGEGQVQMDKEKPVVLPSTLANMPLKTGHTLTTYQPEESTASNQLISQNVTKAKEISYEGMKIELTKSTETVMKNLLLTTQTPKLPVTVHLLNGVSEHPEEEVMQSSSSSSDSSKHGLPAPETFREVSADAAVTYKPALKDLSNNTAFPNVELENSFLESITTSHGHVQETKEMPKDATEKTTLFEEFISKDVIPSSFFHSDAEDLLEEETPEKVKELNQLDGNSADGSLLWIHSIPSSIPHESKTGGVFGADGEASVWPISPPPSADKTVKLQTVQPKSTTISSNLVNEYSGPGSISDSNKQTINTEDVNANELVTPPFLLLDVTNGSDFLIGQGEGSVEGSAIQIPGQDLCKSNPCLHGGTCYPRDSFYICTCMPGYSGDRCEIEIDECQSSPCRNGATCIDGVNTFACLCLPSYVGALCEKDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSQEEQLFVNRIGHDYQWIGLNDKMFENDFRWTDGNMLQYENWRPKQPDSFFSSGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENANTFGKMKPRYEINSLIRYHCKDGFIQRHVPIIRCQGNGRWDLPKITCMNPSSFQRTYSKKYYYKNYSPGKVNSFNSSKHFHRWIRTWQDSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBA1</accession>
    <name>A0A6I9XBA1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 850-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106540832</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911571.1">
        <property type="nucleotide sequence ID" value="XM_014056096.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540832"/>
    <dbReference type="KEGG" id="tsr:106540832"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000029">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000464">
        <property type="entry name" value="Zinc finger and SCAN domain containing 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000198">
        <property type="entry name" value="zinc finger protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000848">
        <property type="entry name" value="Zinc finger protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000038">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000785">
        <property type="entry name" value="zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001266">
        <property type="entry name" value="Zinc finger protein 662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000931">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000028">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000275">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF178">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="36"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
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            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
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            <begin position="61"/>
            <end position="88"/>
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        <location>
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        <location>
            <begin position="145"/>
            <end position="172"/>
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    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="200"/>
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    <feature type="domain" description="C2H2-type" evidence="13">
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            <dbReference type="Proteomes" id="UP000504617"/>
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    <sequence length="1021" mass="117114" checksum="D87355B31AA1D814" modified="2020-10-07" version="1">MEKAYECRVCGKAFIRNSHFIIHQRIHKGEKIFECPICGKSFSHNSMLVRHQRTHTGEKPFECPDCGKCFITNSHLTRHQRTHTGEKPFECPICGKGFRDNFSLMTHQNIHTGEKPFECPDCGKTYSQNSQLMNHQRTHTGEKPFECPDCGDSFIRNSHLMRHQSTHTGEKRFECPHCGKGFNQNCELMRHQRTHTGEKRFECLDCGKGFSQNSHLMNHQRIHTGEKPFECPDCGKGFTQNSHLMNHQRTHTGEKPFECSNCGKGFSQNSHLVRHQRTHTGEKPFQCYDCGKSFITNSHLMRHQRTHTGEKPFECLDCGKCFSEDSYLVIHQRIHTGEKPFECSDCGKSFSQNSYLMNHQRTHTGEKPFECPDCGESFIKNFYLIRHQRTHTGDKPFKCLDCGKGFTQNSHLLRHQRTHTGDKPFECPDCGKSFSQNSHLINHQNIHTGEKPFECLDCGKSFSRNSHLTQNSHLVIHQRFHTGEKPFECLDCGKRFSQNSHLVIHQRTHTGEKPFECPICGKSFSDNSSLVTHQRTHTGEKPFECPDCGKSFTQSSHLVTHQRTHTGEKHFECLDCGKRYSQNFYLVIHQRTHTGEKPFECPICGKCFSDNSSLGKHQRTHTGEKPFECLDCGKSFTKNSNMVMHHRTHTGEKPFECTICGKSFNRNSTLVLHQRTHTGEKPFECLDCGKSFHDNSNLVRHQRIHTGEKPFECPICGKGFSDNSSLVRHQRTHTGEKPFECPICGKGFSDNSSLVTHQRIHTGEKPFECPDCGKSFCQNSSLVIHQRTHTGEKPFECPDCGKGFSRYSSLVIHQRTHTGEKPFECPDCGKGFSRYSSLVIHQRTHTGEKPFECPDCGKSFNQNSDVVQHQRTHTGEKPFECPDCGKGFSQNSHLEVHQRTHTGEKPFECPDCGKGFSQNSNLVIHQRTHTGEKPFECPDCGKGFSNRWXNSHLMRHQRTHTGEKPFECSDCGKCYSQNSYLMIHQRTHTVEKSLNCPICGHYFKHKSSLIAHKVIHMRKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBT2</accession>
    <name>A0A6I9XBT2_9SAUR</name>
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        <submittedName>
            <fullName evidence="5">LOW QUALITY PROTEIN: retinol dehydrogenase 14-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106540943</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
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        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014056261.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106540943"/>
    <dbReference type="KEGG" id="tsr:106540943"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
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        <property type="entry name" value="RETINOL DEHYDROGENASE 14"/>
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    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU000363"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013911736.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="32068" checksum="1DFA46070135A9CC" modified="2020-10-07" version="1">MPAGGPMEGKTAIITGANSGLGRATAAELLRQGARVIMACRDCGRAEEAARELRAEVGLCSRGGGECRGELVVHELDLASLRSVRSFCQQVLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLGHFLLTNLLLSLLKSSAPSRIVVVSSKLYKHGEINFDDLNSELSYNKSFAYSRSKLANILFTRELARRIQNTGVTVNVLHPGIVRTNLGRYVHIPLLAKPLFNLVSWAFFKSPSEGAQTIVYLSTSPEVEGVSGKYFATARRSSCCPRPQMTWWQEKCGISVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBW5</accession>
    <name>A0A6I9XBW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Heterogeneous nuclear ribonucleoprotein H isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HNRNPH1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908673.1">
        <property type="nucleotide sequence ID" value="XM_014053198.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106538644"/>
    <dbReference type="KEGG" id="tsr:106538644"/>
    <dbReference type="CTD" id="3187"/>
    <dbReference type="OrthoDB" id="431068at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="InterPro" id="IPR012996">
        <property type="entry name" value="Znf_CHHC"/>
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    <keyword evidence="9 13" id="KW-0687">Ribonucleoprotein</keyword>
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    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
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            <dbReference type="RefSeq" id="XP_013908673.1"/>
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    <sequence length="518" mass="56545" checksum="6EAD0082F184108A" modified="2020-10-07" version="1">MDPCHTEETGPEIPGLATEGETETTLTSGMMLNAEGGEGYVVKVRGLPWSCSADEVQRFFSECKILNGSSGVRFIYTREGRPSGEAFVELETEEDVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMTMQRPGPYDRPGVGRGYNSLGRGSGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGREWTLLSAGMSDHRYGDGSSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGGSGGAYGSQMMGAMVKETEGVVQDWNTNTLAGSQSSYGGPANQQLSGGYGGGYGGQSSMSGYVWQMPSDQDLDPIHTKLVLASHGNQSAMNSSYYSSGNRASMGVNGMAGMSNMSNMSGGWGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCF1</accession>
    <name>A0A6I9XCF1_9SAUR</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="TGFBR3/Endoglin-like_N"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027120582" evidence="2">
        <location>
            <begin position="19"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="TGFBR3/Endoglin-like N-terminal" evidence="3">
        <location>
            <begin position="45"/>
            <end position="211"/>
        </location>
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    <feature type="domain" description="TGFBR3/Endoglin-like N-terminal" evidence="3">
        <location>
            <begin position="215"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF26060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013911532.1"/>
        </source>
    </evidence>
    <sequence length="348" mass="38689" checksum="E342772E47DA9244" modified="2020-10-07" version="1">MTSYCILPAFALMIGCLAFPGPVPRSGCLLSQVNSSHPVQALLESFTVLSGCASKGTAGLPQEVHILNVRNAEDAGHHEREVVLHLNPIASMHIHQKPLVFLLNSPVPLIWKVKTERLALGIQRLFIVSAGSMVQFEKEGFSLTAKREEKPLPQGNKHLLEWAEKEYGAVTSFTEFKISRNVYIKVGEVPFFPTTCNIEKNFISLNYLAGYLQPKTAEGCIMSSVAHDKEVHIIELIAPDSNSAFQVDIIIDIRPSRPDTTLVRNLVLILKCRKSVNWVIKSHDVQGRLEAITSNGVGFGKETERSMTMSKTIVFDMPSSHENLIAWAYEHGHYPVTTYTKAPLANRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCI3</accession>
    <name>A0A6I9XCI3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Suppressor of cytokine signaling 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SOCS2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as EPOR and GHR. Specifically recognizes and binds phosphorylated proteins via its SH2 domain, promoting their ubiquitination. The ECS(SOCS2) complex acts as a key regulator of growth hormone receptor (GHR) levels by mediating ubiquitination and degradation of GHR, following GHR phosphorylation by JAK2. The ECS(SOCS2) also catalyzes ubiquitination and degradation of JAK2-phosphorylated EPOR.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Substrate-recognition component of the ECS(SOCS2) complex, composed of SOCS2, CUL5, ELOB, ELOC and RNF7/RBX2. Interacts with IGF1R. Interacts with DCUN1D1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911971.1">
        <property type="nucleotide sequence ID" value="XM_014056496.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106541148"/>
    <dbReference type="KEGG" id="tsr:106541148"/>
    <dbReference type="CTD" id="8835"/>
    <dbReference type="OrthoDB" id="10063348at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000002">
        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000049">
        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF7">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="8" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="46"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="17">
        <location>
            <begin position="149"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013911971.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="22373" checksum="24225AACF3C927BD" modified="2020-10-07" version="1">MTLRSAESSDGTDRAGPQWEAGGASAEPAEGERLDSAMRELRCAGWYWGNMSVAEAKERLQDTSEGTFLIRDSSHSEYLLTISVKTSVGPTNLRIEFQGGKFRLDSVICIRSKLQQFDSVVHLIEYYVLMCKDRRMVSEIPSNGTVHLHLNKPLYHSTLSLQHQCRIAINRHTSKIQELPLPTRLKEFLKEYRYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEE5</accession>
    <name>A0A6I9XEE5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Angiomotin-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">AMOTL2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the angiomotin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909483.1">
        <property type="nucleotide sequence ID" value="XM_014054008.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909484.1">
        <property type="nucleotide sequence ID" value="XM_014054009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEE5"/>
    <dbReference type="GeneID" id="106539249"/>
    <dbReference type="KEGG" id="tsr:106539249"/>
    <dbReference type="CTD" id="51421"/>
    <dbReference type="OrthoDB" id="5974715at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003365">
        <property type="term" value="P:establishment of cell polarity involved in ameboidal cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009114">
        <property type="entry name" value="Angiomotin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051747">
        <property type="entry name" value="Angiomotin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024646">
        <property type="entry name" value="Angiomotin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826">
        <property type="entry name" value="ANGIOMOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826:SF3">
        <property type="entry name" value="ANGIOMOTIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12240">
        <property type="entry name" value="Angiomotin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01807">
        <property type="entry name" value="ANGIOMOTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Angiomotin C-terminal" evidence="14">
        <location>
            <begin position="474"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="39"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="299"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="532"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="602"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="709"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013909483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909484.1"/>
        </source>
    </evidence>
    <sequence length="759" mass="86105" checksum="88BFBFCE35BB258F" modified="2020-10-07" version="1">MRTAEDSSGTVLHRLIQEQLRYGNLTENRTLLAIQQQALRGGGSSGSPQSSLESLTQDESQMVQQSTRQEPQGQEHHADHFYLENSMYRFYQPQHKGEELPTYEEAKAQSQYYATQPGQHVRATLSGAPIEGGQRRTYASDINSIPQDESLKELKHGHIRSLSEQLLQVSLERNGIQAQNHMSSSYSYPQLSRHHQLCLSRGKSSEASDELRGPPPEYPYIIPSQEASESYFSDPRQHYREGPGFQHPEIRMMQTPMSQAFMSQQAALCHNHLGSLTSAGVAALMATQAASTSNHLSQIEAVLMENKKLLRENETFQRENELLRRELENYNEKANRIQKLESEIQQISEDYENLMKASLKRETLEKTMRNKKDGEMRRLQDFNRDLKERLESANKQLANRAQENQEGNQGTMAKLVAQNYEYQQEREKLQREIARLHGTNEDQRRRAELLEQALSSAQARTAKMEDELQKKRAYVEKVERLQQALGQLQAACEKREQLELRLRTRLEQELKILRAQQRQMGAPGGGSPELNAHILSEQLREREEKILALEADMTKWEQKYLEECTMRQFAMDAAATAAAQRDTTIINHSPRHSPNSSFDEDLLLANHKHQEMENRLKELHAQILEKDAVIKVLQQRSRKDPNKAHQGSLRPAKSVPSVFAASAIQCWPGNSVSESDYHSNPDNKALKAEAEVTAPSSCIPFHTKHGSKDGSTQTEGLPTNTPCDELKLHVEKNSGAASSLGMLPAAKILDLSDMVEILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEW5</accession>
    <name>A0A6I9XEW5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">MED19</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912412.1">
        <property type="nucleotide sequence ID" value="XM_014056937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEW5"/>
    <dbReference type="GeneID" id="106541470"/>
    <dbReference type="KEGG" id="tsr:106541470"/>
    <dbReference type="CTD" id="219541"/>
    <dbReference type="OrthoDB" id="10044050at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019403">
        <property type="entry name" value="Mediator_Med19_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536">
        <property type="entry name" value="LUNG CANCER METASTASIS-RELATED LCMR1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10278">
        <property type="entry name" value="Med19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="167"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013912412.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="26083" checksum="62D739280C066DB3" modified="2020-10-07" version="1">MMENFSALFGGTEPPPSTTAAALGFGPGKPGGPGTGQPPVPTVTPVGEETARKAAAASGPFYLMRELPGTTELTGSTNLITHYNLEHAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPICGSSFNPITGTMLTGFRLHAGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPETPSDSDHKKKKKKKEDDPERKRKKKEKKKKKNRHSPEHPGVGSSQASSSSSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XF55</accession>
    <name>A0A6I9XF55_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 220 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMEM220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914294.1">
        <property type="nucleotide sequence ID" value="XM_014058819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XF55"/>
    <dbReference type="GeneID" id="106542948"/>
    <dbReference type="KEGG" id="tsr:106542948"/>
    <dbReference type="CTD" id="388335"/>
    <dbReference type="OrthoDB" id="9924288at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029377">
        <property type="entry name" value="TMEM220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34262">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34262:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15071">
        <property type="entry name" value="TMEM220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914294.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="19618" checksum="D18FC2B3C158411C" modified="2020-10-07" version="1">METECFASPPLPGASRLWRLSNLIMAVFFGLAGAVQINDPDPGLWIVVYSVPASLALVISLNPSVTDNIVWRSISDLHSTACVVGSAVLGYSLLTTAQSNILHEEEGRELFGLVIVTAWMSLCRNSANKLCLFLSRNSLGGFHLVVAIFLCLSPFVMWLYIYVNTEMRASWPAHCKTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFG1</accession>
    <name>A0A6I9XFG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SETD3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-histidyl-[protein] + S-adenosyl-L-methionine = N(tele)-methyl-L-histidyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19369"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:11600"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16367"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.85"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class V-like SAM-binding methyltransferase superfamily. SETD3 actin-histidine methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013914404.1">
        <property type="nucleotide sequence ID" value="XM_014058929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFG1"/>
    <dbReference type="GeneID" id="106543038"/>
    <dbReference type="KEGG" id="tsr:106543038"/>
    <dbReference type="CTD" id="84193"/>
    <dbReference type="OrthoDB" id="441812at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19176">
        <property type="entry name" value="SET_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000001">
        <property type="entry name" value="histone-lysine N-methyltransferase setd3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000001">
        <property type="entry name" value="histone-lysine N-methyltransferase setd3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025785">
        <property type="entry name" value="SETD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044428">
        <property type="entry name" value="SETD3_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51565">
        <property type="entry name" value="SAM_MT85_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="94"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="540"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="560"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013914404.1"/>
        </source>
    </evidence>
    <sequence length="592" mass="67216" checksum="A67B6F063289CDAC" modified="2020-10-07" version="1">MGKKSRVKTQKSGTGATTAVSPKEILNLISELLQKCSSPTPGPGKEWEEYQQIRALVERIRKKQKGLSIIFDGKREDYFSELIKWAGENGAATDGFEVANFGEEGFGLKATQDIKAEELFLWVPRKLLMTVESAKNSVLGSLYSQDRILQAMGNITLAFHLLCERHNPNSFWLPYIQTLPNEYNTALYFEEDEVEYLQSTQAIHDIFSQYKNTARQYAYFYKVVQTHPNASKLPLKDSFTYDDYRWAVSSVMARQNQIPAEDGSRVTLALIPLWDMCNHTNGLITTGYNLKDDRCECVALQDFKPGEQIYIFYGTRSNAEFVIHSGFFFDNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFALHSTEPPISAQLLAFLRVFCMTEDELKEHLIGEHTIDRIFTLGNSEFPVSWENEVKLWTFLEARASLLLKTYKTTVQDDKFILETQDLTRNATMAIKLRLGEKEILEKAIKSASANREYYSKQMAEEAPLPRYEESNVALLENTVADSSLPIVLRNLDEETEEPQDLKIEESIGAEANGFVNGDSSLLNRTKLENEKLNQEESSRETEDTKESSSDSTDEVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGY8</accession>
    <name>A0A6I9XGY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PSMB2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910288.1">
        <property type="nucleotide sequence ID" value="XM_014054813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGY8"/>
    <dbReference type="GeneID" id="106539898"/>
    <dbReference type="KEGG" id="tsr:106539898"/>
    <dbReference type="CTD" id="5690"/>
    <dbReference type="OrthoDB" id="268428at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000008">
        <property type="entry name" value="Proteasome subunit beta type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013910288.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22764" checksum="45DD59C5BFF0C05F" modified="2020-10-07" version="1">MEYLIGIQGPDFVLVAADTIAASSIVHMKRDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADYLRSRSPYHVNLLLAGYDEQDGPALYYMDYLASLAKVPFAAHGYGAFLTLSILDRYYKPGITREEAIEVLKKCLEEIQKRFILNLPSFNARFIDKDGIHEVTNVPLLKSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHN5</accession>
    <name>A0A6I9XHN5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 15-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106543700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915239.1">
        <property type="nucleotide sequence ID" value="XM_014059764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHN5"/>
    <dbReference type="GeneID" id="106543700"/>
    <dbReference type="KEGG" id="tsr:106543700"/>
    <dbReference type="OrthoDB" id="694479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF159">
        <property type="entry name" value="CD180 MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013915239.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="46969" checksum="F79B7FB5DDD590A4" modified="2020-10-07" version="1">MAYSVAQALPLTRLDPSSRPALVATNSVEVEQEALRADGLSDIVIQTIQASQRPSTTRICNATWRAFSKWCSSSNIVPLANVLDFLQQGLIKGLSPNMLRCQVAALSTVLTLPTKHLDLSQNNLYSIPWKSLSSLWKLQVLLLSNNSIHKVRDRTFISLGRLHKLDIGRNDISFLGNSFSVGLISLSELILAYNKLQELQYKSFQYFENLQKLNLQNNNISSIEMGTFRSLTRLRQLYLQNNCLHALHNRLFSMLQHLEILNLEGNMIKTIAPGAFIPLSHLTVLNLIHNKIEHIKFKTLLSLQTPGTHILLSDNPWFCDCDLQRVFAKLHSVRRLILDDYYNMTCMEPHLLRNLPLSAVDTELCTAETVTVLVITFTVFVTVVAAIVMAERNRKKRAEKRWNEDSDVSYNAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIU4</accession>
    <name>A0A6I9XIU4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ERLEC1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lectin involved in the quality control of the secretory pathway. As a member of the endoplasmic reticulum-associated degradation lumenal (ERAD-L) surveillance system, targets misfolded endoplasmic reticulum lumenal glycoproteins for degradation.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Probable lectin that binds selectively to improperly folded lumenal proteins. May function in endoplasmic reticulum quality control and endoplasmic reticulum-associated degradation (ERAD) of both non-glycosylated proteins and glycoproteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the OS-9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913842.1">
        <property type="nucleotide sequence ID" value="XM_014058367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIU4"/>
    <dbReference type="GeneID" id="106542587"/>
    <dbReference type="KEGG" id="tsr:106542587"/>
    <dbReference type="CTD" id="27248"/>
    <dbReference type="OrthoDB" id="239053at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.130.10:FF:000001">
        <property type="entry name" value="Endoplasmic reticulum lectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.130.10:FF:000003">
        <property type="entry name" value="Endoplasmic reticulum lectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045149">
        <property type="entry name" value="OS-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012913">
        <property type="entry name" value="OS9-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414">
        <property type="entry name" value="OS-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07915">
        <property type="entry name" value="PRKCSH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026697471" description="Endoplasmic reticulum lectin" evidence="8">
        <location>
            <begin position="30"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="9">
        <location>
            <begin position="114"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="9">
        <location>
            <begin position="347"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913842.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="55922" checksum="9F7EBEBD015F5530" modified="2020-10-07" version="1">MKRRKLEPRRGAALVPLLLWGLLEAKTAAAGGGSRSLPQFSDDVPFRVHWPGREFILPTAGVLYKEDYYIIMTTADKEKYKCILPVLANGDEEEDKDYKGPSPAELLEPLFKQSSCSYRIESYWTYEVCHGKHIRQYHEEKETGQKINIQEYYLGNTPRKSPPLDPDQEENEIEKDTAKEIPTKNIEGQMTPYFPIRMGNGTPCSLRQNLPRSSTVMYVCHPEAKHEILSVAEVTTCEYEVVILTPLLCSHPKYRFRASPVNDIFCQSLPGSPLKPHKLEHLEQQQEMMKMPFRRAKEEEISSAKEEKFSSFHKPVAVGTSQLLTVGTTHISKLTDEQLIKEFLSGSYCFHGGVGWWRYEFCYGKYVHQYHKDKENGKTTIVVGTWNKEEHLEWAQKNVARTYHQKDDDLQTVKMVSHYYGNGDVCDLTDKPRQVTVRLKCKESDSPHAVTIYMVEPHSCQYILGVESPVICKILDTADEYGLLSVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIX3</accession>
    <name>A0A6I9XIX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger MYM-type protein 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106542706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913991.1">
        <property type="nucleotide sequence ID" value="XM_014058516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIX3"/>
    <dbReference type="GeneID" id="106542706"/>
    <dbReference type="KEGG" id="tsr:106542706"/>
    <dbReference type="OrthoDB" id="10060419at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF22">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013913991.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="52557" checksum="B055A717EC5D8DA9" modified="2020-10-07" version="1">MAEKRKCRQYSTEHLKLRFIPSPSNVQLPFCFLCEKTFSNEAMKPSRLKDYLVRIHSDKADKPVSFFQSLKAKFEGCSTVEKLFGKSSLNADKGLICSYEVSLLIAKCGKPHTIGETLILPAVKQIVATMLGLSASTFTQSIPLSNDTVSKRIDEMAAEVEEMLIDTLKSTKFVMQIDESTISENEALLLAYVRFINRNEEIGEELLFAQTLATDTKGSSIFKKVEEFFNEKNIPLTNVMACATDGAASMVGRYRGFQAHLKSVLYEENDKEFERLLLHTEVRWLSKEKCLRRFHKLFDTVVEFLESINPSLSSSLKLQHLAVAYLADVFDKLNEVNVKLQGNKINFIKAKGVICSFISKLDILASNMSRQKLYQFPSLGNERQLQAEDLNIFFCHLKQLKEDMETRFYDLRNLEVPTWVLNPVTADFVQLHPKLQEPLTDLKYDYESQSHFQSYGCEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLN9</accession>
    <name>A0A6I9XLN9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arylacetamide deacetylase-like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106544881</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916759.1">
        <property type="nucleotide sequence ID" value="XM_014061284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLN9"/>
    <dbReference type="GeneID" id="106544881"/>
    <dbReference type="KEGG" id="tsr:106544881"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017157">
        <property type="entry name" value="Arylacetamide_deacetylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF32">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037251">
        <property type="entry name" value="Arylacetamide_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="5">
        <location>
            <begin position="85"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="5">
        <location>
            <begin position="296"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR037251-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013916759.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="43185" checksum="DF11CECE92D2995C" modified="2020-10-07" version="1">MDLHLGDKEPLKIWLLHLLVIWTFNLGNICEKLGLCGQMKFVRFVSSLTPAGKDPNIYLRDTTFENVPVTIYQPKRTNPGLRKGLVFLHGGCYMFGSKNIYNGLCCYLSRESDSVVVFVDYRLGPEHQYPAQLTDAKATILHFMKHAEDYGVNPNHILLAGDSSGGALAAALCQEFVKIGNQPKIRAQILIYPFLQRIDYMLPSHFQNQHGPLLTRKRYLSIPLKLLNQKGVDKEKMGRNGHVPEDLRQKFKKLINADLLPSEFKARGYNPPMPCPFSKELYEATNMKELTMRSLIIGEDDIIKQFPETYILTCEYDLLRDEGFLYKKRLEDNGVPVTWNHLQEGFHGFITFINSRVFEFTFTKPAVENMVSFIKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPJ4</accession>
    <name>A0A6I9XPJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106544239</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013915937.1">
        <property type="nucleotide sequence ID" value="XM_014060462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPJ4"/>
    <dbReference type="GeneID" id="106544239"/>
    <dbReference type="KEGG" id="tsr:106544239"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF8">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="339"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013915937.1"/>
        </source>
    </evidence>
    <sequence length="575" mass="67102" checksum="6F1FB25CB96E30A1" modified="2020-10-07" version="1">MKNRSTLDKVVKIIAGDNQSIVLRLEEKNAYTVMQTLATVEEEKVAKWLSNSDRSCWNNIKWNINLIFSSAACINGSFLQQREEKFRTFEKTVGVDMSAVLLFFKKIAAKPKVFAKVKSALKILLHCPLHAPTSPEALRFFLVVPVLLQNQDFDLLSQLAEAIYKLPQKGKQALETLWSNLEVCFFKDVVSMFQRFIGVNMFLKYKQNSDWCFNKRGCIMSLKVFQMLYEVNCRSGFKIHESNFYVPEVKKIILNLDRFPLDFNFLLRFKSACSDINLALYVFSQFPFILNLEDKIVVHETHCKILDLEYLATYNPPSTMTVREQDLLQDTWRYLRNAEEICFRQSFKVYFEGQPDIKYGGLIQDFFTIISSQLCIPKAQVFRHFEDSHHIWFSHEVSTEEDIYFLIGNLFGIALQNKSIIALPFPLALFKKLANIEPTLEDLKELSPDIGRILQLILDERYDDNFKSMMLDFTVVEERKGSRIEVELKENGANIPVTLYNRKEYVDAYVNYIFNDSVKKQFEDFARGFQRGCPSATWKMFLPLELRNILFGHTTYNWDQLEKVECAPLPQSLHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPP1</accession>
    <name>A0A6I9XPP1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger protein 507</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ZNF507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917989.1">
        <property type="nucleotide sequence ID" value="XM_014062514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPP1"/>
    <dbReference type="GeneID" id="106545841"/>
    <dbReference type="KEGG" id="tsr:106545841"/>
    <dbReference type="CTD" id="22847"/>
    <dbReference type="OrthoDB" id="10066771at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF74">
        <property type="entry name" value="ZINC FINGER PROTEIN 507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="618"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="783"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="439"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="744"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917989.1"/>
        </source>
    </evidence>
    <sequence length="823" mass="91326" checksum="E8FE6C200BBD42F7" modified="2020-10-07" version="1">MEEGSNIIALISNLREQEAVLISEAIVSSSADNADDSLKCKPDPLIQVIQKLSKIVESEKSQKCVLIGKKRLYPDTTGLPEVPSKALELPALGTINIEDYYTMDDPPQSKRKVLCYECSLCKSVSPSLPLLQEHMKQHGQQNEVMLMCSVCSFTTRSVEDFESHIRSHPENSSKIHALLKEEHGVEVSGTALPGPVEKVIISTDHEMQPPPQVPEKGRRKWYTYEQYGMYRCLICSYTCGQQRMLKTHAWKHAGEVNCSYPIFEEEKKQAALSDSAILDTVVLSLDNNEAGLCNNSPVQLRICSPEPMAFKTEELVKEESALSQPAALSPADRLFEESASLHEDLDPDHLGADSLLSSAQKIIHCSPNKKGHVNVIVERLPSAEEPALQNEVMDSVLHYSVDAGPEHPPAGRDASDDVYHMDKNSIEIEEVIIGWSSTEKAEENMNPSRAKTADENAPPARRRTNSESLRLHSLAAEALVTMPIRAAELARSSFRAFADVNPLGSEAGQRPPEGGYLGHSKRIASLKTEELSSLHQEERALVELQKDKPELSEVPIKMGISMSLLTVIEKLKERTDQNASDDDILKELQDNVQCQPSGDAGVLGSSLVEYIPDAERPFRCRLCHYSSGNKGYIKQHLRVHRQRQPYQCPICEHVADHSKDLESHMINHCKTRMYQCKQCEESFHYKVNKAALCLKMNNFFPLSCRLLGQLTGKKVTKTLEGDAVPFTSSSEGLSFSNSPKPTANKILSSDHHQDQASTTNTSSGSEKPAGLSYLGSEYCVLLFCCCICGFESTSKENLLDHMKEHEGEIINIILNKDHAATQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQS7</accession>
    <name>A0A6I9XQS7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein fosB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FOSB</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918414.1">
        <property type="nucleotide sequence ID" value="XM_014062939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQS7"/>
    <dbReference type="GeneID" id="106546156"/>
    <dbReference type="KEGG" id="tsr:106546156"/>
    <dbReference type="CTD" id="2354"/>
    <dbReference type="OrthoDB" id="5866312at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14721">
        <property type="entry name" value="bZIP_Fos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000006">
        <property type="entry name" value="fos-related antigen 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF3">
        <property type="entry name" value="PROTEIN FOSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="105"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918414.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="26704" checksum="0F69460B5793970C" modified="2020-10-07" version="1">MPGSFVPTVTAISTSQDLQWLVQPTLISSMSQSQQQQQQQQQQPSGLPPPTVDPYDLPGPSYAAPGMGAYGAGPSTAPVPEATPPRSSRARPRRTREETLTPEEEEKRRVRRERNKLAAAKCRNRRRELTDRLQAETDQLEEEKAELESEIAELQKERERLEFVLVAHKGTCKIPYQEITELSGQPGPSAEELTDPYPVNPSYTSSFVFTYPEGATCGASHQRNGSSDLSPDSLNSPSLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQZ0</accession>
    <name>A0A6I9XQZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Toll-like receptor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28 29 30" type="primary">TLR3</name>
    </gene>
    <organism evidence="27 30">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28 29 30">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913090.1">
        <property type="nucleotide sequence ID" value="XM_014057615.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913091.1">
        <property type="nucleotide sequence ID" value="XM_014057616.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913093.1">
        <property type="nucleotide sequence ID" value="XM_014057618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQZ0"/>
    <dbReference type="GeneID" id="106542018"/>
    <dbReference type="KEGG" id="tsr:106542018"/>
    <dbReference type="CTD" id="7098"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090594">
        <property type="term" value="P:inflammatory response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032722">
        <property type="term" value="P:positive regulation of chemokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032728">
        <property type="term" value="P:positive regulation of interferon-beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032755">
        <property type="term" value="P:positive regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043330">
        <property type="term" value="P:response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000008">
        <property type="entry name" value="Toll-like receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000137">
        <property type="entry name" value="Toll-like receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041015">
        <property type="entry name" value="TLR3_TMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017241">
        <property type="entry name" value="Toll-like_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF524">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17968">
        <property type="entry name" value="Tlr3_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037595">
        <property type="entry name" value="Toll-like_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
    <keyword evidence="22" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="18 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 25" id="KW-0732">Signal</keyword>
    <keyword evidence="9 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636611" description="Toll-like receptor 3" evidence="25">
        <location>
            <begin position="25"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="26">
        <location>
            <begin position="740"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00072834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013913090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013913091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013913093.1"/>
        </source>
    </evidence>
    <sequence length="890" mass="102198" checksum="1794EC7CC5240CB7" modified="2020-10-07" version="1">MRKLLKCWTCLPFILFIIPTNCGSTQNICKITDKMADCSHLKLYQIPSNLPADITTLDISHNQLKTLLPENLTKYNQLVYLNVGFNSISKLGIHLCLSLPLLEVLRLEHNQLRTLYGEVFTSCTNLRELNVGFNILNTKNDPFKNLKNLAFLDVSHNHLSSTKLGSQQQLGNLQELVMSQNQITKLNKEDLYFLGNSSLKRLDLSSNPIKEFQPGCFQAIENIYGLDLNNVPLGPDGTEKLCLELSGTKIQNLSMSKVQISRISNVTFSGMSKTNLTILNLSNNYLSVIENASFIYFSNLKELNLMNNNITKLSSHTLYGLQNLNYFNLENSHVRIIDSAFQWLTHLNYLIMDGNKFLEITPHTFKGLENLKNLSLCQCNINSITNMTFASLANSSLQFLNLTKAGIISIKPKAFSWLRQLKMLDLGLNNIKQNLTGQEFQGLSNIETLYLSYNYQLNLTSESFTLIPSLRKLRMRKVRCSNLAITPSPFRKLKNLTILDMGNNNIANIKDDIFDGLHQLEILDLQHNNLARLWKHTNPDGPVLFLKDLHNLRLLDLESNGFDEIPHKAFYGLSLLRSLNLDSNLLNLLPKSVFDDLTSLSFLFLQKNMVTAVDEKVFGAVFKHLKVLNMASNPFDCTCDSIPWFVTWLNSNKTYISNLNNYQCNTPPKYHSNLVAQFDTSICDNAPFKLVFILCTTAILLLIFIAMLVHFQGWRIKFMYTVAVNRVLGIKEIDRQQQQFEYDAYIIHAKKDMNWVSNNFIPLENNQPAIRFCLEERDFEAGISEFEAIVNSIQRSRKIIFIVTKHLLEDPWCKRFKVYHAIQQAIEQSRDSIILIFRHDIPDYKLKNALCLRRAMFKSRCILEWPVQKERLDAFHQQLKSALQSNSRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRH5</accession>
    <name>A0A6I9XRH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106537649</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907315.1">
        <property type="nucleotide sequence ID" value="XM_014051840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRH5"/>
    <dbReference type="GeneID" id="106537649"/>
    <dbReference type="KEGG" id="tsr:106537649"/>
    <dbReference type="OrthoDB" id="9025762at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15915">
        <property type="entry name" value="7tmA_OR12D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013907315.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="35241" checksum="099A70C4D2459EAA" modified="2020-10-07" version="1">MKNHTEVEEFIFLGLNNDLELQYIFFAIFLLLYMATLVGNGAIVIVVLAEPRLHSPMYFFLGNLSCLDIFHSTITIPKMLSGFLTEPQTISFNGCMVQLFFLYFLGSSEGILLGIMAYDRYVAICNPLRYTVTMRKECCVLMATATLLTGFFHALMHAVAMAHLPFCGPNLIEHFVCDIKPLLKLACGSTRLNLMLLSIVTSCVVMGPFIFILLSYLYIITFLLCKVHSQHSLQKAFFTCGSHLTVVALLYIPVLFNYMLPTVGTSSQRDMVITLIYSAVTPVLNPLIYTLRNQEVKVGMKKFFGKKSYTFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRN1</accession>
    <name>A0A6I9XRN1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin-protein ligase NEURL1B</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Neuralized-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Neuralized-like protein 1B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Neuralized-like protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase NEURL1B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">NEURL1B</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">E3 ubiquitin-protein ligase involved in regulation of the Notch pathway through influencing the stability and activity of several Notch ligands.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013918714.1">
        <property type="nucleotide sequence ID" value="XM_014063239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRN1"/>
    <dbReference type="GeneID" id="106546375"/>
    <dbReference type="KEGG" id="tsr:106546375"/>
    <dbReference type="CTD" id="54492"/>
    <dbReference type="OrthoDB" id="6078042at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16786">
        <property type="entry name" value="mRING-HC-C3HC5_NEU1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000005">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000020">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000056">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF10">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NHR" evidence="23">
        <location>
            <begin position="43"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="23">
        <location>
            <begin position="281"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="504"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="473"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="482"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013918714.1"/>
        </source>
    </evidence>
    <sequence length="556" mass="61816" checksum="3F00B399E9477BD2" modified="2020-10-07" version="1">MGNTAHKSLADTSHQARPVSHRPYYTLPNGNHERRSVLTITEPPRFHFQAKGKNIRLDTHSRKAIRRNSFCNGITFTNRPIHLYEKVRLKLVSVHHGWSGALRFGFTIHDPSQMNAGDIPKYACPDLVTRPGFWAKALPERFALRDNVLAFWVDRQGRVFYSINDEEPILFHCGVKVSGPLWALIDVYGITHEVQILDSMFAETMTSTRLSNARLSTCLPQSNHDSANFNNNELENNQVVAKIANLNLSRMPGLPTDNHTIPCCPSRRQLGQIVPAFLDTDLHFHPTHGPDINFSQDRMSACTNWQESNRTLVFSDRPLHIGESLFVEVGHLGLPYYGALTFGITSCDPGTLRTNELPADPDFLLDRKEYWVVCRGFPVLNNGDILNFVLLPNGEVHHGINGMSRGMLMCVDTSQSLWVFFSIHGVINQLKILGTVQSSPVTISPSGSPGGSQYDSDSDMAFSVNRSSSASESSLVTAPSSPLSPPVSPVFSPPEPSSSKNGECTVCFDNEVDVVIYTCGHMCLCNTCGLKLKKQLNACCPICRRVIKDIIKIYRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSY0</accession>
    <name>A0A6I9XSY0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein phosphatase 1 regulatory subunit 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PPP1R27</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916851.1">
        <property type="nucleotide sequence ID" value="XM_014061376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSY0"/>
    <dbReference type="GeneID" id="106544953"/>
    <dbReference type="KEGG" id="tsr:106544953"/>
    <dbReference type="CTD" id="116729"/>
    <dbReference type="OrthoDB" id="71307at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053080">
        <property type="entry name" value="PP1_regulatory_subunit_27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916851.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="16993" checksum="C9D71E6CBF21C80D" modified="2020-10-07" version="1">MKYYCPSSLPRYRRYARRLKTVHFPNDVLFQDHIRQGDLEQVGRFIRARKVSLDTIYPSGMAAIHEAVLAGNLECVKLLIKYGADINQTDDEGWTPLHMACSDGYADIARYLISLGAQCDATNNEGDKPSDLIDPEYKDLVELFQAAHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTD8</accession>
    <name>A0A6I9XTD8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SLC30A1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913958.1">
        <property type="nucleotide sequence ID" value="XM_014058483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTD8"/>
    <dbReference type="GeneID" id="106542685"/>
    <dbReference type="KEGG" id="tsr:106542685"/>
    <dbReference type="CTD" id="7779"/>
    <dbReference type="OrthoDB" id="29444at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019855">
        <property type="term" value="F:calcium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010312">
        <property type="term" value="P:detoxification of zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF1">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="26"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="11">
        <location>
            <begin position="346"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013913958.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="55557" checksum="64EB42F6EBC33802" modified="2020-10-07" version="1">MGVESPGERPVEARRRHGRRRVRLMCMLALTFGFFVVEVVVSRLTSSLAMLSDSFHMLSDVMALVVALVAVRFAQRTRATKKNTFGWVRAEVMGALVNAVFLTALCFTIFLEAIERFTEPHEIEQPLVVIGVGAAGLLINVLGLCLFHQHGGGHGHSHGGSGGGSHRRNGSRGGKAEPPPGDLHKEETNTLVDNFNSSNGVSLEKLGDVVKDSQMELQPNGNISSNTSIDMEMDEDTSAQLNMRGVFLHVLGDALGSVIVVVNAIIFYSYWTPCPEVGDCINVCVNDPCTNFTQLSPLERDQIPVAGPCWVLYLDPSLCLIMVCILLYTTYPLLKESALILLQTVPKQIDIRSLNEKLRKLEDVEAVHELHIWQLAGSRIIGTAHIKCRDPESYMKVAKDIKEIFHDEGIHATTIQPEFAIVSSEESVTKCEFPCRTQCALKQCCGTVEKAMGKKIEKSPSVSISCSEILIDSPHHRARRTKSECTPAVKLEAEPNPNKQFESSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUX8</accession>
    <name>A0A6I9XUX8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vesicle-associated membrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106543086</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914468.1">
        <property type="nucleotide sequence ID" value="XM_014058993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUX8"/>
    <dbReference type="GeneID" id="106543086"/>
    <dbReference type="KEGG" id="tsr:106543086"/>
    <dbReference type="OrthoDB" id="10042941at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000013">
        <property type="entry name" value="Vesicle-associated membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914468.1"/>
        </source>
    </evidence>
    <sequence length="116" mass="12666" checksum="D8AE3A1DD6DFF914" modified="2020-10-07" version="1">MSANAAAMPAAAGGEAGGPPPPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVM1</accession>
    <name>A0A6I9XVM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein kinase greatwall</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Microtubule-associated serine/threonine-protein kinase-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">MASTL</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013908665.1">
        <property type="nucleotide sequence ID" value="XM_014053190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVM1"/>
    <dbReference type="GeneID" id="106538640"/>
    <dbReference type="KEGG" id="tsr:106538640"/>
    <dbReference type="CTD" id="84930"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000278">
        <property type="entry name" value="serine/threonine-protein kinase greatwall isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000277">
        <property type="entry name" value="serine/threonine-protein kinase greatwall isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000484">
        <property type="entry name" value="Serine/threonine-protein kinase greatwall, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="36"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="23">
        <location>
            <begin position="858"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013908665.1"/>
        </source>
    </evidence>
    <sequence length="901" mass="100597" checksum="28F47A2F1F061AB5" modified="2020-10-07" version="1">MAETFVPQDDVRREEEKGASCGKRIAVPQPPSIEDFTIVKPISRGAFGKVYLGRKGGKLYAVKVVKKADLINKNMAHQVQAERDALALSKSPFIVHLFYSLQSANNVYLVMEYLIGGDVKSLLHICGYFDEEMAIKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSRVTLNREINMIDILTTPSMSKPMQDYSRTPGQLLSLISSFGFYTPVGGKGPVQSPSPVSADMPQLSREFVSPLSISCKDYSMASSKLLRACIDSSAVTPGMPIRSLTPTLLQSRRRYGTSSASSHSRTQLSSMESECCSSPRWEKDIQEEDNFCPAPGQMNAMEKHEAADMPLVNNSKVLKQKLLETAISPISSNDSTDKHNGRSEYIDSSDISVRICDVKDLVRKCLSEYKEWEGTFCTNNSVQSKDVTKPNCHFSHLGLDNLKKEEKERPVLQKTGIKRCFELVDTSPSQERILVKKNNSEYKRGCKIIEFPEKKSTSLTAEICCLKLEGCLQESCPKKSQTKDSNCLEENKENKSGAPLIAKNLMNELDADYEKGGKHTNFSFISIDDEKPLVSMNIDSDLSLPEISATENHLEKPTFDLNGSIKDVPLEESKSESMLSLPCCSRWETRDQPMLPVQESKSLDRNQDKSLKYITEKHTSVHSSPMEMLKALKKNIVAFRSYNSSINMSNMTEPSKISMLSVEGMNISACSGSYPMTITPAQKGTPYRIYQTPQGNVGTPYRTPKSVRRGAAPVVGERILGTPDYLAPELLIAKTHGAAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNSQNAIDILLTVDSAKRAGLKELKLHPLFHGMDWDNLHNQTMTFIPQPNDETDTSYFEARNNAQQLTVSGFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVR4</accession>
    <name>A0A6I9XVR4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger protein 830</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Coiled-coil domain-containing protein 16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZNF830</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920254.1">
        <property type="nucleotide sequence ID" value="XM_014064779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVR4"/>
    <dbReference type="GeneID" id="106547560"/>
    <dbReference type="KEGG" id="tsr:106547560"/>
    <dbReference type="CTD" id="91603"/>
    <dbReference type="OrthoDB" id="77607at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059039">
        <property type="entry name" value="ZNF380_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040050">
        <property type="entry name" value="ZNF830-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13278">
        <property type="entry name" value="ZINC FINGER PROTEIN 830"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13278:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 830"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23406">
        <property type="entry name" value="ZNF380_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="16">
        <location>
            <begin position="50"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="323"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013920254.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="41888" checksum="17197E168D18C076" modified="2020-10-07" version="1">MATSSSGREPRRSVNTLGQEELRRLMRAKQRESAAGKRKIDSPYVRYNSLGNLSCCLCNVIVKNELLWQTHVLGKQHKENIKYSTSKSFPETSSPLKKKKLEPRILDQKKGQDCNDQAQAVSSNLPKTFFDKIEQTEVEIISSKSSSVVLLGNYKTEEGKKEDMTKEIRERDSKTSLPTPAQYELSSDLVPKAELVACSSTDYLENNALATPQLSHSESVQKAEPQEKTHQRRENTAEALPEGFFDDPEVDAKVRKVDTPKDQMDKEWDEFQKAMRQINTISEAIVAEEDEEGRLDRQIGEIDEQIECFRRVEQLRDRQEIRKDQLKEAMSQTVTQSKEDDDIDSNDEDELQDLLSQDWRAKGTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVW9</accession>
    <name>A0A6I9XVW9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box only protein 38-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106545788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917921.1">
        <property type="nucleotide sequence ID" value="XM_014062446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVW9"/>
    <dbReference type="GeneID" id="106545788"/>
    <dbReference type="KEGG" id="tsr:106545788"/>
    <dbReference type="OrthoDB" id="10036898at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22107">
        <property type="entry name" value="F-box_FBXO38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042354">
        <property type="entry name" value="FBX38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14753">
        <property type="entry name" value="F-BOX ONLY PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14753:SF3">
        <property type="entry name" value="F-BOX ONLY PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013917921.1"/>
        </source>
    </evidence>
    <sequence length="429" mass="48820" checksum="F33EA9855FCD868C" modified="2020-10-07" version="1">MGPRRKNMKVYSTSRESPVCIKAAELKDYMNQLSHEVLCHIFRYLPLQDIMCMECLSRKLKEAVILYLRVVKVVDLCAGRWWEYMPTGFTDSSFLTLLKKMPDIEQLYGLHPRHLDRRRVRGYEAFSIPGVLEALQACPNLLGVETSHLELVEAIWTYMPQVHILGKFRNRNGAFPIPPENKLKIPIGAKIQTLHLVGVNVPDIPCIPMLRHLYLKWVRLTKPQPFKDFLCNSLRTFVMRNCAGPTNSLKYVPLVTGLASARNLEHLELVRVPFLGGLIQHVVEDSWRSGGFRNLHTIVLGACKNALEVDLGYLIITAARRLHEVRIQPSLTKDGVFSALKMAELEFPQFETLHLGYVDEFLLQCKMINADLVKYGLADVVENPGIITDIGMKAVNEVFSSIKYLVIYNCPHLHNPSSWITGIVLTNMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXB7</accession>
    <name>A0A6I9XXB7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106548070</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106548070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920859.1">
        <property type="nucleotide sequence ID" value="XM_014065384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXB7"/>
    <dbReference type="GeneID" id="106548070"/>
    <dbReference type="KEGG" id="tsr:106548070"/>
    <dbReference type="OrthoDB" id="9680533at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920859.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="26211" checksum="A2A339FC0BED9E77" modified="2020-10-07" version="1">MEMHQCWDSQWQEFLKNVAGLPIPPPPLENNGTDSQASSGRDKEDPQQKSSGEARRESVLQGDPNISGKACWMGREKLNSSVKVKVEEEEVNLESQAPQPFSSQDAEEPQKMSSKRKNPSSTEARRKRHRKAVDLNLKMKIIKAYEAGKKMHKIAKEEGLACSTICATVKDRERIREALKGAIGMNTNITIIQKRHTPEMEPLLILWIRDRIQKRLAFSFRLRHAAFSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXC0</accession>
    <name>A0A6I9XXC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING finger protein 150</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RNF150</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915263.1">
        <property type="nucleotide sequence ID" value="XM_014059788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXC0"/>
    <dbReference type="GeneID" id="106543719"/>
    <dbReference type="KEGG" id="tsr:106543719"/>
    <dbReference type="CTD" id="57484"/>
    <dbReference type="OrthoDB" id="5357315at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02122">
        <property type="entry name" value="PA_GRAIL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16805">
        <property type="entry name" value="RING-H2_RNF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000016">
        <property type="entry name" value="Ring finger protein 150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF27">
        <property type="entry name" value="RING FINGER PROTEIN 128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026841723" description="RING finger protein 150" evidence="13">
        <location>
            <begin position="35"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="270"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013915263.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="47249" checksum="A6939EF49FABE56E" modified="2020-10-07" version="1">MAMSVIQACRSLALSTWLLSFCFVHLLCLDFTVAEKEEWYTAFVNITYPDPSGGGSELRSEKTECGRYGEHSPKNEVWGQVVMTSAAADRQTCDPNARFAVPGSGKLWIALVPKGNCTYKDKIRHAASQNASAVVIYNVGSSNANETITMPPAGIDDVVAIMIPEPKGKEIVSLLERNITVMMHITVGTRNLQKYVSRTSVVFVSISFIVLMIISLAWLVFYYIQRFRYANARDRNQRRLGDAAKKAIGKLQVRTIRKGDKETESDFDNCAVCIEGYKPNDVVRILPCRHLFHKSCVDPWLLDHRTCPMCKMNILKALGIPPNADCMDDIPPDFEASIGGLPTNQITGASDITVNESSIAVDTPIRTVGVLQVIQDADPLPQAGESNNTTSSEQEPAVSSDSDISLIMAMEVGLSDVELSTDQDCEEVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXW4</accession>
    <name>A0A6I9XXW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Adrenal ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FDX1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915438.1">
        <property type="nucleotide sequence ID" value="XM_014059963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXW4"/>
    <dbReference type="GeneID" id="106543864"/>
    <dbReference type="KEGG" id="tsr:106543864"/>
    <dbReference type="CTD" id="2230"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF26">
        <property type="entry name" value="ADRENODOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="14" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="12" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="17">
        <location>
            <begin position="55"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013915438.1"/>
        </source>
    </evidence>
    <sequence length="172" mass="19039" checksum="8A696D1598C4BEC8" modified="2020-10-07" version="1">MATSGFRLLFRAAPSSCWRLLEAPFRAGSRWNNAARLGSCRSFQTATGLSSEEKVTVHFVNRDGDKLTAKGKVGDSLLDVVIDNNLDIDGFGACEGTLACSTCHLIFEDHIFEKLDAITDEEMDMLDLAYGLTERSRLGCQICLKKYMNNMTVRVPEAVADARQHIDMSKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ71</accession>
    <name>A0A6I9XZ71_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alpha-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Maltase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106544207</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013915888.1">
        <property type="nucleotide sequence ID" value="XM_014060413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ71"/>
    <dbReference type="GeneID" id="106544207"/>
    <dbReference type="KEGG" id="tsr:106544207"/>
    <dbReference type="OrthoDB" id="5839090at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1760:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000016">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017957">
        <property type="entry name" value="P_trefoil_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF133">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00025">
        <property type="entry name" value="P_TREFOIL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="P-type" evidence="13">
        <location>
            <begin position="18"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="13">
        <location>
            <begin position="984"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915888.1"/>
        </source>
    </evidence>
    <sequence length="1140" mass="130349" checksum="DE5C1CFF310B9722" modified="2020-10-07" version="1">TLIVLLATGYSGLKAFSPTCPVIPLPERIDCIPDQVATKDLCVLRGCCWDPQNETHVPWCYFSKNHGYQVQGRMSDTNPGFEATLTRLPSPSLFGNDIEKLHLTAEYQTANRFHFKITDPANKDRFEVPHENVQPFQGSKATNLMYRVEVTDNPFGISVIRNSNNKVLFNTSIGPLIYADQFLQLSIRVPSWNVYGVGEHVHRQYRHDFNWKTWPIFTRDAFPNGDMSNLYGAHTFFLCLEDTSGHSFGVFLMNSNAMDFALQPAPAVTYRTIGGVLDFYVFLGNTPEEVVQQYLLLIGLPWMPSYWNLGFHICRWNYTDLNDVKAVVERNRAAGIPYDVQYTDIDYMEDKKDFTYDKINFAGLPEFVQDLHDHGQKYVIILDPAISVNNLKNNTPYEAYLRGEQMKVWINESNGTSPLIGEVWPGTTVFPDFSSPEAVQWWVYECNNFHKTINFDGLWIDMNEVSNFVKGSKTGCASNNLNYPPFTPKILDGVMYSKTVCMDVVQKAGKHYDVHSLYGYFMSIATDKALKTIFRESRSFLLSRSTFSGSGKFTGHWLGDNFATWNDIKWSIPGILEFNLFGYPFIGADICGFIDDTSEELCRRWLQLGAFYPFSRNHNAAGHAPKDPAYFGQNSLLVNSTKHYLTIRYTLLPYLYTLFYKAHARGETVARPVLHDWDSQLPGFTFSDMFIQISTRLPSQFVYGFGETEHKQFRHEMNWKTWPMFARDQPPGYRFNTYGVQPFYMGLEEDGNAHGVLLLNSNGMEVKFQPTPALTYRTIGGILDFYMVLGPTPEQVVQEYTALIGRPVLPAYWGLGFQLCRYGYKNDAEISELYDNMKAANISYFQGVDKVTAYIPDAVWYEYETGLKAPCRKQECQMFLPENKLGLHLRGGYIFPIQEPANTTVYRYFLILKVLTMAVIHKRYIEPNNLIFENIKILGLPLEPSDVKVTSNNVVQSYNLTVKYNAADKVAHITGLRLKLGEEHTLSWKQTLKDIDKFDCHPEENASKEKCEALGCIWEKASVAGAPYCYYPPDNGYTVDQIEYTSSGLTANLGTNINSVRLSGQQIPLIDKLRLEVKYHDNNMLQFKIYDYSKKRFEVPVPLNLPQTPISTLENRLYEVSIQNHPFGIQVRRKSTGTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZR8</accession>
    <name>A0A6I9XZR8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NDUFV3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919441.1">
        <property type="nucleotide sequence ID" value="XM_014063966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZR8"/>
    <dbReference type="GeneID" id="106546955"/>
    <dbReference type="KEGG" id="tsr:106546955"/>
    <dbReference type="CTD" id="4731"/>
    <dbReference type="OrthoDB" id="6161911at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042775">
        <property type="term" value="P:mitochondrial ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026193">
        <property type="entry name" value="NDUFV3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17117:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17117">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15880">
        <property type="entry name" value="NDUFV3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919441.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36837" checksum="A2D395789CC94562" modified="2020-10-07" version="1">MAASLVTRYGRVAAFKAISQQAWGVRSMPSLYLCTKPGNSKKGTPKDKGKVITNCKPDAAVKLTDEDLRKFLAMKTLVMFPQKAEFPFERTPVFSSTEGLGKRLTDEELLSSSSSESDSSSDSEDDDFKEKPHRHKELCWTTDNKQLESDMIKTASQQRSKEIHLKSKNLGIVNAENQKPIEVSSGSQEVSLSETDPRRTQLVPPQNISQNLEQDEKITKKMQKTEMQERIFEEPRPEVSRERMPVIKTTLKEETIPEEAQIEKQSTPQVARPSPETAQETHDISTYKNLQHHEYQPFTFVDFDVLLSKFRLPQPSSGRLSPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZU9</accession>
    <name>A0A6I9XZU9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ETS-related transcription factor Elf-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106546995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919486.1">
        <property type="nucleotide sequence ID" value="XM_014064011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZU9"/>
    <dbReference type="GeneID" id="106546995"/>
    <dbReference type="KEGG" id="tsr:106546995"/>
    <dbReference type="OrthoDB" id="8196042at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919486.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="36034" checksum="BDD2A2E3A6420C32" modified="2020-10-07" version="1">MPKDIIYIDDVDPNSGLDSPDSALLSTPGANRSKAKASSNPGSKGSGPLLKLPFNAKSAKLKQPLETVAQPLPPLTSEVLTTMQSSHSPHAAQLYRTVHLMPSVQSIQEGHAAVTGNLLDESLNSSVPNIRTIQTSGQVPVVVSPGNQQLHTVTLQTVPLTTVIASADPNSSATPQKFILQAIPSSQPMTVLKENVVLQSSKSVSPPSSIVFSPAQVHQVLTSNVQTICNGTVNVASSSSSPSPSFSTGASVVTFTPGGSHGAAPQPASNAVITSVIKAPETKTAMVQGTVKLEPENSFGGEDDPPLEASFHQQPFVVMVSASNGFSPAVHIKEESEQFQLPSPYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y106</accession>
    <name>A0A6I9Y106_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BAIAP2L2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910430.1">
        <property type="nucleotide sequence ID" value="XM_014054955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y106"/>
    <dbReference type="GeneID" id="106539994"/>
    <dbReference type="KEGG" id="tsr:106539994"/>
    <dbReference type="CTD" id="80115"/>
    <dbReference type="OrthoDB" id="9944156at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027681">
        <property type="entry name" value="IRSp53/IRTKS/Pinkbar"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14206">
        <property type="entry name" value="BRAIN-SPECIFIC ANGIOGENESIS INHIBITOR 1-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14206:SF5">
        <property type="entry name" value="BRAIN-SPECIFIC ANGIOGENESIS INHIBITOR 1-ASSOCIATED PROTEIN 2-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="IMD" evidence="5">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="320"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="265"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013910430.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="55244" checksum="735BCA42D4CFE425" modified="2020-10-07" version="1">MEQSYRSTITIYKSILEQFNPALEKLIYLGNKYLQAFYALSEAADVYFTALQKIGEQALQSSTSQILGEILIQMSESQIHLNRNLEIVAETFHVDLLQHMEKNTKLDVQFINDSWQQYEKEYRHRVASIDKSMMDLRRMESRRDKNVWELKENMVHLRSELQVFVSESQQAAELEEKRRYRFLAEKHQLLSNTLLQFYTKARTLILNKAPLWKEELEASHNPVQNVLNSPPSQRQPTGHLTPTHLENLPRLPTDFGPIMARQSFSQEPLQMTPSAQPETKRKTTPRTPSLGSASGLSRSTSFGEQAAWRAEADNKEGNSSHPLKMQAIVPYVTGSNQTLLPLSVGDVVTLLLPEAQNGWLYGKLEDTSMSGWFPEAYVKPLEESTPRGFPLRSSRSVDNLLDQSTASAPDNYWQKSSRPQSSLSSPSSVGEGTLSNSISNAATPSMGSKKPATQDHPPELFPRGTNPFATVKLRPTVTNDRSAPIVRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y183</accession>
    <name>A0A6I9Y183_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">COP9 signalosome complex subunit 7a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">COPS7A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920156.1">
        <property type="nucleotide sequence ID" value="XM_014064681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y183"/>
    <dbReference type="GeneID" id="106547480"/>
    <dbReference type="KEGG" id="tsr:106547480"/>
    <dbReference type="CTD" id="50813"/>
    <dbReference type="OrthoDB" id="10265275at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010387">
        <property type="term" value="P:COP9 signalosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041481">
        <property type="entry name" value="CSN7_helixI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF7">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18392">
        <property type="entry name" value="CSN7a_helixI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="10">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="227"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013920156.1"/>
        </source>
    </evidence>
    <sequence length="275" mass="30477" checksum="9182E6C731EA7BC5" modified="2020-10-07" version="1">MAAEVKVSGQNQEQFLLLARSAHGAALASLIHQVLEAPGIYVFGELLDMPNVQELAESEFSPVFRLLTVFAYGTYSDYLAEAGNLPPLTEAQKNKLRHLSVVTLASKLKCIPYSVLLEQLQLKNVRQLEDLVIEAVYADVLRGSLDQRNQRLEVDYSIGRDIRREELSIITRTLQEWCQGCEVVLSSIEEQVSRANQHKEQQLGLKQQIESEVANLKKTIKVTTAAAAAATSQDPEQHLSELREPAPGTNQRQASKKASKGKGLRGSAKIWSKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1J9</accession>
    <name>A0A6I9Y1J9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DERL2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916863.1">
        <property type="nucleotide sequence ID" value="XM_014061388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1J9"/>
    <dbReference type="GeneID" id="106544962"/>
    <dbReference type="KEGG" id="tsr:106544962"/>
    <dbReference type="CTD" id="51009"/>
    <dbReference type="OrthoDB" id="1716531at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000016">
        <property type="entry name" value="Derlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013916863.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="27662" checksum="B740D9BDA32B9CB9" modified="2020-10-07" version="1">MAYQTFRQEYLQVPPVTRAYTTACVLTTAAVQLELITPFQLYFNPELIFKNFQVWRLITNYLFFGPVGFNFLFNMIFLYRYCRMLEEGSFRGRTADFVFMFLFGGLLMTLFGLFVNLVFLGQAFTIMLVYVWSRRNPFVRMNFFGLLIFQAPFLPWVLMGFSLLLGNSIIVDLLGIAVGHIYFFLEDVFPNQPGGGRLLKTPYILKLIFDTPEEDPNYNPLPEERPGGFAWGEGQRLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1Y4</accession>
    <name>A0A6I9Y1Y4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin--tyrosine ligase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106545091</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamyl-[protein] + L-glutamate + ATP = gamma-L-glutamyl-L-glutamyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60144"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143622"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:60145"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917028.1">
        <property type="nucleotide sequence ID" value="XM_014061553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1Y4"/>
    <dbReference type="GeneID" id="106545091"/>
    <dbReference type="KEGG" id="tsr:106545091"/>
    <dbReference type="OrthoDB" id="2016263at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF145">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917028.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="25843" checksum="C13BDBD7020AB7BA" modified="2020-10-07" version="1">MKQQDMPVGMARDLEETDSSSEEEEEMEGPEEHPCIMWTGGFRRIPIMVFHAEAILTKDSYIRLIGERYHLSFKIVRTDSRLVRSILSAHGFREVHPSSNEYNLMWTGSHLKPYVLRTLTDIQKVNHFPRSYELTRKDRLYKNINRMQQIYGFKTFHILPQTFILPAEYQEFCTSYSKDRGPWIVKPVASSRGRGVYLINNPNQISLEENILVSRYINNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y326</accession>
    <name>A0A6I9Y326_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">N-terminal EF-hand calcium-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NECAB1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917598.1">
        <property type="nucleotide sequence ID" value="XM_014062123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y326"/>
    <dbReference type="GeneID" id="106545526"/>
    <dbReference type="KEGG" id="tsr:106545526"/>
    <dbReference type="CTD" id="64168"/>
    <dbReference type="OrthoDB" id="289247at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042984">
        <property type="term" value="P:regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000025">
        <property type="entry name" value="N-terminal EF-hand calcium-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039862">
        <property type="entry name" value="NECAB1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178:SF11">
        <property type="entry name" value="N-TERMINAL EF-HAND CALCIUM-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="34"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="ABM" evidence="6">
        <location>
            <begin position="257"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="216"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917598.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="40683" checksum="E07DB43B0A08CE87" modified="2020-10-07" version="1">MDGVESPDAAPASSSSSSSPESSEELRSALTMTKGLSIFLDILRRADKNDDGKLSFDEFKAYFADGILSIEELHQLFQKIDIHNTDNLETEELFEYFSQHLGAYESVLSALEDLNVAILKAMDTTKKNYQEASTLEQFVTRFLLKETLNQLQSLHNSLECAMETTEEQTQQQQQVPKKPEVLKIQPPGRRSGRRFQRNNSFSPVSPLLNPGLHKKDNQWMSQINRLQKLIDKLEQKDLKLEPLEEEVSEDNTKSQIMIVQRQMSVITEELEPFRFALKQYVDMASSQDGCLHVSVQKLSGKSCFIVYEFWKNNAWNSHLQMNYSKSFQRSNVDCLETPEHITTMLVPASWCILNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3C1</accession>
    <name>A0A6I9Y3C1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">SNF-related serine/threonine-protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">SNF1-related kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">SNRK</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">May play a role in hematopoietic cell proliferation or differentiation. Potential mediator of neuronal apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013917748.1">
        <property type="nucleotide sequence ID" value="XM_014062273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3C1"/>
    <dbReference type="GeneID" id="106545637"/>
    <dbReference type="KEGG" id="tsr:106545637"/>
    <dbReference type="CTD" id="54861"/>
    <dbReference type="OrthoDB" id="942095at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14074">
        <property type="entry name" value="STKc_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14339">
        <property type="entry name" value="UBA_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000166">
        <property type="entry name" value="SNF-related serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF90">
        <property type="entry name" value="SNF RELATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 26" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="16"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="24">
        <location>
            <begin position="291"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="377"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="26">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013917748.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="48829" checksum="7E962215AD2AC224" modified="2020-10-07" version="1">MAGFKRGYDGKIAGLYDLDKTLGRGHFAVVKLARHVFTGEKVAVKVIDKTKLDTLATGHLFQEVRCMKLVQHPNIVRLYEVIDTQTKLYLILELGDGGDMFDYIMKHEEGLNEDLAKKYFAQIVHAISYCHKLHVVHRDLKPENVVFFEKQGLVKLTDFGFSNKFQPGKKLTTSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILYMLVCGQPPFQEANDSETLTMIMDCKYTVPSRVSKECKDLITQMLQRDPKRRASLEEIENHTWLQGVDPSPATKYNIPLVSYKNLSEEEHNSIIQRMVLGDIADRDIIVEALETNKYNHITATYFLLAERILREKQEKEIQTRSASPSNIKAQFRQSWPTKIDVPQDLDNDFTASPLTHGAVPQSPARSAENVLNGHRSKALNVSAKKEDIPELAGSTLSVVPSVNLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y484</accession>
    <name>A0A6I9Y484_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Macrophage mannose receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106546316</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918633.1">
        <property type="nucleotide sequence ID" value="XM_014063158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y484"/>
    <dbReference type="GeneID" id="106546316"/>
    <dbReference type="KEGG" id="tsr:106546316"/>
    <dbReference type="OrthoDB" id="6356110at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015721">
        <property type="term" value="P:bile acid and bile salt transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23407">
        <property type="entry name" value="beta-trefoil_Ricin_MRC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000032">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052678">
        <property type="entry name" value="OST-beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36129:SF1">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36129">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24562">
        <property type="entry name" value="CysR_MRC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type-II" evidence="7">
        <location>
            <begin position="210"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918633.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="36522" checksum="5E923F822A77158E" modified="2020-10-07" version="1">MASLGDEATSPRLGHNSYQNYYLTMPSNRHHNKILGLANQDCSLSLESCKQNGPSHCGQLSPVFQISDNDTFMMYNENLDRCLKLQESRSFTFDICNKFNEWQNFKWVSDHQLLNMAVKLCLSVPSKSNMVPVTLSRCNKTTELQKWECRNDTLFALVNQELFLNPANGRENGVILSKKPSTKSVWKIYGTKKSLCAQKYEALFTIEGNSLGAPCVFPFKYMNKWHAKCIVDDDEHKRFWCGTTPDVDEDSLTGYCPIKDEKDNFFWVKNHWTGDFYQINSLSALTWDQARKSCWQQNSELLSINELYEQAYLTGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4F0</accession>
    <name>A0A6I9Y4F0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium/potassium/calcium exchanger 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLC24A4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918818.1">
        <property type="nucleotide sequence ID" value="XM_014063343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4F0"/>
    <dbReference type="GeneID" id="106546467"/>
    <dbReference type="KEGG" id="tsr:106546467"/>
    <dbReference type="CTD" id="123041"/>
    <dbReference type="OrthoDB" id="2127281at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000005">
        <property type="entry name" value="sodium/potassium/calcium exchanger 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF21">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="176"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013918818.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="38887" checksum="C537D0BFE626421E" modified="2020-10-07" version="1">MYFFYILIMKFNMKMQKFFTIKSMNVGNGNTVNSELEDVKGTQEPGRNSVVMVDEIICSSPPKYRFPEAGLRIMITNKFGPRTRLRMASRLIINERQRLIHSSNGQLIQNGRHENIENGAIHAENQEEAGHIAQSPFSPPEGKMEKVKWILTWPLILLLFFTIPNCSKPRWENFFMVTFLFSTLWIALFSYFMVWMVTVIGYTLGIPDVIMGITFLAAGTSVPDCMASLIVARQGLGDMAVSNTIGSNVFDILVGLGLPWGLQTMAVHYGSYVKINSKGLVYSVVLLLGSVALTVGGIHINKWKLDRKLGIYVLTLYAIFLCFSILIEFNVFTFVNFPMCREDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y539</accession>
    <name>A0A6I9Y539_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Photoreceptor outer segment membrane glycoprotein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106541795</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRPH2/ROM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912805.1">
        <property type="nucleotide sequence ID" value="XM_014057330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y539"/>
    <dbReference type="GeneID" id="106541795"/>
    <dbReference type="KEGG" id="tsr:106541795"/>
    <dbReference type="OrthoDB" id="9836210at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03162">
        <property type="entry name" value="peripherin_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000002">
        <property type="entry name" value="Retinal outer segment membrane protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000830">
        <property type="entry name" value="Peripherin/rom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018498">
        <property type="entry name" value="Peripherin/rom-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042026">
        <property type="entry name" value="Peripherin_LEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF184">
        <property type="entry name" value="PERIPHERIN 2 LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00218">
        <property type="entry name" value="PERIPHERNRDS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00930">
        <property type="entry name" value="RDS_ROM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912805.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="41772" checksum="464771CB29F9CE37" modified="2020-10-07" version="1">MAVLKVKFTKTKRDKLAQILWILNWFSVISGAVVFSLGLFLKIEIRKRKEVIAKWDINSVPNTLISIGAIVCMINFFGGKICYDCTDANKFNRWKLIMLPYIVCTLCFTFCIFVGAILCYTMRNELEESLYLGLGNAIKFYKDTDIPGRCFLKRTIDTLQIRFQCCGNNGFRDWFEIEWVSPRYLDMTSEDVLDRLKGNIDGKFLVDGVPFSCCNPNSPRPCIQYQLRNNTAHYRYDFMTEELNIWMRGCREALLDYYTDIMRSIGITTFVVWVFELLVLIGVRYLQTSMRNLLLQVDPLCDSDGWLFENSFVEIVMWNITKINHLGKGNQVSRALDNSNMDVCTANHNSENTTPKYIPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5S8</accession>
    <name>A0A6I9Y5S8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RAP2A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013920058.1">
        <property type="nucleotide sequence ID" value="XM_014064583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5S8"/>
    <dbReference type="GeneID" id="106547415"/>
    <dbReference type="KEGG" id="tsr:106547415"/>
    <dbReference type="CTD" id="5911"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032486">
        <property type="term" value="P:Rap protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04176">
        <property type="entry name" value="Rap2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000189">
        <property type="entry name" value="Member of ras oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041840">
        <property type="entry name" value="Rap2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013920058.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20643" checksum="5E731A6EB6FA3226" modified="2020-10-07" version="1">MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDASPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIRPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQMNYASQPDKDDPCCSACNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5U1</accession>
    <name>A0A6I9Y5U1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RAF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013913455.1">
        <property type="nucleotide sequence ID" value="XM_014057980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5U1"/>
    <dbReference type="GeneID" id="106542299"/>
    <dbReference type="KEGG" id="tsr:106542299"/>
    <dbReference type="CTD" id="5894"/>
    <dbReference type="OrthoDB" id="774951at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000024">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000036">
        <property type="entry name" value="RAF proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF22">
        <property type="entry name" value="RAF PROTO-ONCOGENE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="56"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013913455.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="40258" checksum="BC5759433C6441F9" modified="2020-10-07" version="1">MSGSPNNTSPTGWSQPKTPVPAQRERAAGANPQEKKIRPRGQRDSSYYWEIEASEVMLSTRVGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYNHLHVQETKFPMLQRIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEDRTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDSNPFTFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYTSPDLSKLYKNCPKAMKRLVADCVKKVREERPLFPQILSSIELLQHSLPKINRSASEPSLHRATHTQDINSCTLTSTRLPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6D6</accession>
    <name>A0A6I9Y6D6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">UBE2L3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013913945.1">
        <property type="nucleotide sequence ID" value="XM_014058470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6D6"/>
    <dbReference type="SMR" id="A0A6I9Y6D6"/>
    <dbReference type="GeneID" id="106542671"/>
    <dbReference type="KEGG" id="tsr:106542671"/>
    <dbReference type="CTD" id="7332"/>
    <dbReference type="OrthoDB" id="9973183at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23801">
        <property type="entry name" value="UBCc_UBE2L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000011">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013913945.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17862" checksum="F5A30243BE3C9985" modified="2020-10-07" version="1">MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y735</accession>
    <name>A0A6I9Y735_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PX domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PXDC1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921228.1">
        <property type="nucleotide sequence ID" value="XM_014065753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y735"/>
    <dbReference type="GeneID" id="106548396"/>
    <dbReference type="KEGG" id="tsr:106548396"/>
    <dbReference type="CTD" id="221749"/>
    <dbReference type="OrthoDB" id="9933228at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040288">
        <property type="entry name" value="PXDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31433">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31433:SF0">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013921228.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="15126" checksum="3DFAABEF0F82D04E" modified="2020-10-07" version="1">MASAVFEGTSLVNMFVRGCWVNGIRRLIVNRRGDEEEFFEIRTEWSDRNVLYLHRSYSDLGRLFKRLLDSFPEDRPELSQSPFLQGLPAASFTDFLPLPPPLPNSILCPLGDWSLRREEVPCKGAALPSRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y777</accession>
    <name>A0A6I9Y777_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Valine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106543251</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the attachment of valine to tRNA(Val) in a two-step reaction: valine is first activated by ATP to form Val-AMP and then transferred to the acceptor end of tRNA(Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013914695.1">
        <property type="nucleotide sequence ID" value="XM_014059220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y777"/>
    <dbReference type="GeneID" id="106543251"/>
    <dbReference type="KEGG" id="tsr:106543251"/>
    <dbReference type="OrthoDB" id="629407at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF71">
        <property type="entry name" value="VALINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="15">
        <location>
            <begin position="129"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013914695.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="35956" checksum="9B9A99E778B7C770" modified="2020-10-07" version="1">MPSAHLNSASHRNMSVVCRLLLWAEKRSWDGFGGWRRSYSQGSKPDLNKQVGKMDHLKNKEQRQRRLWKKMEAIEAQVAAGPKEPSNQDKTLSSKNIAEYDIPTIAGQKKDTSAPLPASYSPRYVEAAWYPWWVKEGFFKPECQQHLPHRKPETFSISLPPPNVTGSLHLGHALTVAIEDTLVRWRRMQGYRVLWLPGSDHAGIATQAVVEKKIWKERGVLRQNLSRDEFLQEVWKWIEEKGNEIFKQLKEMGASLDWDRACFTMDSRFSEAVTEAFVQLHEQGLIYRDRRLVNWSCALQSAISDIEVENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y788</accession>
    <name>A0A6I9Y788_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tumor necrosis factor receptor superfamily member 12A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TNFRSF12A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914750.1">
        <property type="nucleotide sequence ID" value="XM_014059275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y788"/>
    <dbReference type="GeneID" id="106543300"/>
    <dbReference type="KEGG" id="tsr:106543300"/>
    <dbReference type="CTD" id="51330"/>
    <dbReference type="OrthoDB" id="9010896at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13413">
        <property type="entry name" value="TNFRSF12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022316">
        <property type="entry name" value="TNFR_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32037">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32037:SF2">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12191">
        <property type="entry name" value="stn_TNFRSF12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01962">
        <property type="entry name" value="TNFACTORR12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026710376" evidence="2">
        <location>
            <begin position="21"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914750.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="12922" checksum="09FC15AB15E1BC33" modified="2020-10-07" version="1">MLAWGVILGLLPVLLGSTRGEPIAETTCPGGQSWSPDLEKCMDCSVCQRQNKNDFCSTCGDPQPTNSFNLWVLIGASLGAVLLASIIAGTIIYARCRRREKFTTPIEETGGHSAQESLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y835</accession>
    <name>A0A6I9Y835_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Semaphorin-3F-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106544480</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the semaphorin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916235.1">
        <property type="nucleotide sequence ID" value="XM_014060760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y835"/>
    <dbReference type="GeneID" id="106544480"/>
    <dbReference type="KEGG" id="tsr:106544480"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF28">
        <property type="entry name" value="SEMA DOMAIN, IMMUNOGLOBULIN DOMAIN (IG), SHORT BASIC DOMAIN, SECRETED, (SEMAPHORIN) 3GA ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="81"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916235.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="31316" checksum="9AAE744E7427FF1C" modified="2020-10-07" version="1">MGVSQLTLHRCREYGEACADCCLARDPYCAWDGQRCARYSHTAKRRSRRQDIKHGDPLRQCRGFNAKAEQQLSEQIQYGLEGGSVFLECEPRSPHATIKWLTQTDDSTKRKVLPRDRILLQTPQGVLLGPVMPSDSGLYQCVGTENRFRHTLRRLRLHVLPRALLEGAITSEEAPTLGICESYWQQFNRAQDIQHSQGGHQSHTGPSLWSSEQQSNWYRAAQQHSLNWTTSSPHHSQSTSTRRRPHNHRPHHHHHHHHRSKPWAGHQTPPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8K4</accession>
    <name>A0A6I9Y8K4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Formin-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FMNL2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917080.1">
        <property type="nucleotide sequence ID" value="XM_014061605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8K4"/>
    <dbReference type="GeneID" id="106545146"/>
    <dbReference type="KEGG" id="tsr:106545146"/>
    <dbReference type="CTD" id="114793"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000001">
        <property type="entry name" value="Formin-like 1, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF5">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="17"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="296"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="381"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013917080.1"/>
        </source>
    </evidence>
    <sequence length="484" mass="55825" checksum="E561AB763993D029" modified="2020-10-07" version="1">MASPTVVFNSGLAAVKIKKPIKTKFRMPVFNWVALKPNQINGTVFNEIDDERILEDLNVDEFEEIFKTKAQGPAIDLSSSKQITQKGSNKVTLLEANRAKNLAITLRKAGKTADEICKAIQAFDLKTLPVDFVECLMRFLPTENEVKVLRLYERERKPFENLTDEDRFMMQFSKIERLMQKMTIMAFVGNFTESIQMLTPQLHAIIAASVSIKSSQKLKKILEIILALGNYMNSSKRGAVYGFKLQSLDLLLDTKSTDRKQTLLHYISNVVKEKYQQVCLFYNELHYVEKAAAVSLENVLLDVKELQRGLDLTKREYTMHDHNTMLKEFIQSNEGKLKKLQDDAKIAQDAFDDAVKYFGENPKTTPPSVFFPVFVRFVKAYKQAEEENELRKKQEQALMEKLLEQEALLEQQDQKSPSHKTKRQQQELIAELRRRQVKDNRHVYEGKDGAIEDIITALKKNNITKFPNVHSRILEINPTDEPMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9F4</accession>
    <name>A0A6I9Y9F4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Helix-loop-helix protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NHLH2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918580.1">
        <property type="nucleotide sequence ID" value="XM_014063105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9F4"/>
    <dbReference type="GeneID" id="106546269"/>
    <dbReference type="KEGG" id="tsr:106546269"/>
    <dbReference type="CTD" id="4808"/>
    <dbReference type="OrthoDB" id="10067827at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19701">
        <property type="entry name" value="bHLH_TS_HEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000027">
        <property type="entry name" value="Nescient helix-loop-helix 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040238">
        <property type="entry name" value="TAL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864:SF10">
        <property type="entry name" value="HELIX-LOOP-HELIX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864">
        <property type="entry name" value="T-CELL ACUTE LYMPHOCYTIC LEUKEMIA/STEM CELL LEUKEMIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="72"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918580.1"/>
        </source>
    </evidence>
    <sequence length="130" mass="14374" checksum="026B90BD35A66345" modified="2020-10-07" version="1">MMLSPDQAVDSDHPSSAPSDPESLEAKGLRSCCASDLETPNGEGAGGGRSAAHPPQPLSREEKRRRRRATAKYRSAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9H4</accession>
    <name>A0A6I9Y9H4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Tensin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TNS2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918680.1">
        <property type="nucleotide sequence ID" value="XM_014063205.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546351"/>
    <dbReference type="KEGG" id="tsr:106546351"/>
    <dbReference type="CTD" id="23371"/>
    <dbReference type="OrthoDB" id="6273691at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01213">
        <property type="entry name" value="PTB_tensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09927">
        <property type="entry name" value="SH2_Tensin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000039">
        <property type="entry name" value="Tensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000002">
        <property type="entry name" value="Tensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000010">
        <property type="entry name" value="tensin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035012">
        <property type="entry name" value="Tensin-like_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033929">
        <property type="entry name" value="Tensin_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF1">
        <property type="entry name" value="TENSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="13">
        <location>
            <begin position="34"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase tensin-type" evidence="14">
        <location>
            <begin position="125"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="15">
        <location>
            <begin position="302"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="1259"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="497"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="867"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="919"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="967"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1045"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1180"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="497"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="564"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="922"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1200"/>
            <end position="1210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013918680.1"/>
        </source>
    </evidence>
    <sequence length="1531" mass="169463" checksum="26D38DA866AF0F17" modified="2020-10-07" version="1">MKPRNTVETLLRALGRRESARIPSSCRPGKVSEPHNFKEKVFKKKKKACIVCKESIDAQGLVCRVCKMASHKKCEAKVPSSCQPVPPSELRRNTAPVRRIEHLGSTKSLNTAKQRNTLPRSFSLDHVMERKYDFDLIYITERIISVSFPPALEEQRYRSNLREVAQMLKSKHEDKYLLLNLSEKRNDIIRLNPKVQDFGWPDLHAPPLDKICSICKAMETWLNSDPQHVAVLHCKGNKGKTGVIVASYMYYSKISASADQALSTLTMRKFCEDKVAASLQPSQKRYIHYFSGLLSGTIKMNSNTLFLHHVLLPAIPTFESGGGYQPFLKIYQSMQLVYTSGIYSLHGASGSQKICITLEPALLLKGDVMVKCYHKHLQGGERDVVFWIQFHTCTIHSAQLWFSKDELDEAWRDERFPFEASVEFVFSSSPEKIKGRETYRNNAEVAIDYSISDPIVRWDSYENFNLQYEDSLEDVLHTRGPLDGSLYARVKKKSELSHFSSSNGSPGSLESPPPQGGRLLSISSDSGHSSMLTEKAEDNTPPSRPQPTPAEKEELDRLLGGFGVQSQQPSEGSCSHSVSPPATKERETAILEDELSDVGQPGGSFSYQPQRSGLARHCSCRLDYCSHSPERPRNMSYYRPESVLERRQPIHNGGHSHLHSEGYEEKRRVFRSLSEGLQPHTYPYGHELLPPHSPNRREPNELLILEDQPTFLCPCQDCQEAAREESRLPLASFYNLHLDQEPDFWGVERPPMLHHPLHQGGQPVPILMPASYGPHSMPHHQAFNFESNVMATHLGHAYPSHQRPKVTEDNPEAFAYPRYGSAYHPVPPGYTCGRAPVKSCLSPYTSGYLGSPHSGSISPTSPLYPPARKHGFELPEGNDGYLLSPSTEQPCELQDCREGIPWQDLPTSLQPHRQEVRAACSDESNSPMPVHTSSPVFNNNNNSPGSRTLCHKAASPLENLLQSNLEEQSSPGYKRERMTSSGNPSQILSPMPAFVHFPAFRPQANGAPQRLGLQAPQLCSRNYCASNPPHGPPSAPAKHSPFLERQQHQNGSDACGLSATVPMPRELPPQDALGHQPGPIMERQPQRLTPKEPCPSQPSPGCRTVSGAPASPTTPAIYNGRLQSERDELDTKGLPHGPNLSPSLGPCSLSIQPPALGSGIADMQGSPTPAFPIATAYYTSMEGGSGSREQPREPQQPPLPEKHQATRTWERNSPPGRSPGSAHHVTFAPGVPDGSTPNSDLQQENPVSVKFVQDTSKFWYKPHLSRDQAIALLKDKEPGTFLIRDSNSFQGAYGLALKVITPPPNIINHSTKGDPAEQLVRHFLIETGPKGVKIKGCNNEPHFGSLPALVSQHSITPLSLPCQLRIPSKDLTEERPELAIPTNMSTAADLLKQGAACSVLYLSSVETESLTGPQAVAKATTSILASTPHASASTVHFKVSAQGITLTDNQRKLFFRRHYPVNSVTFCSTDPQDRRWANRDGTTAKIFGFVAKKQGSPCENICHLFAELDPEQPASAIVNFITKVMLGTHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAG7</accession>
    <name>A0A6I9YAG7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable G-protein coupled receptor 173</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GPR173</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Is a receptor for the SMIM20 derived peptides Phoenixin-14 and Phoenixin-20. It mediates the Phoenixin-14 and Phoenixin-20 augmentation of gonadotropin-releasing hormone (GNRH) signaling in the hypothalamus and pituitary gland. In the ovary, it mediates the effects of Phoenixin-14 and Phoenixin-20 induced granulosa cell proliferation during follicular growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920395.1">
        <property type="nucleotide sequence ID" value="XM_014064920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAG7"/>
    <dbReference type="GeneID" id="106547686"/>
    <dbReference type="KEGG" id="tsr:106547686"/>
    <dbReference type="CTD" id="54328"/>
    <dbReference type="OrthoDB" id="6129346at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004968">
        <property type="term" value="F:gonadotropin-releasing hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15217">
        <property type="entry name" value="7tmA_SREB3_GPR173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000074">
        <property type="entry name" value="probable G-protein coupled receptor 173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051509">
        <property type="entry name" value="GPCR_Orphan/Phoenixin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19268">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19268:SF4">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 173-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="37"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013920395.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="41928" checksum="E9A15E8E276D5A13" modified="2020-10-07" version="1">MASANETEEGHGSAPSAASTYAKLLLLGFIICVSLAGNLSLSLLVLKERGLHKAPYYFLLDLCLADVIRSAVCFPFVLVSIRHGSAWTYSVLSCKIVAFMAVLFCFHAAFLLFCISVTRYMAVAHHRFYAKRMTFWTCIAVICMVWTLSVAMAFPPVFDVGTYKFIREEDQCIFEHRYFKANDTLGFMLMLAVLIFATHVVYIKLLLFEYRHRKMKPVQMVPAISQNWTFHGPGATGQAAANWIAGFGRGPMPPTLLGIRQNAHAANRRLLGMEEFKAEKKLGRMFYVITLLFLVLWSPYIVACYWRVFVKACSIPHRYLSTAVWMSFAQAAVNPIVCFLLNKDLKKGLIAHIPCWRTEPELPREPYCVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAH5</accession>
    <name>A0A6I9YAH5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fanconi anemia group J protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106547721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920435.1">
        <property type="nucleotide sequence ID" value="XM_014064960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAH5"/>
    <dbReference type="GeneID" id="106547721"/>
    <dbReference type="KEGG" id="tsr:106547721"/>
    <dbReference type="OrthoDB" id="19182at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920435.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="45500" checksum="C0554CEFC554B668" modified="2020-10-07" version="1">MTMELKEKEESDYYIKFGVDWLSKWIRQQIHHYEDFDCALNSLDAFAKMNQNGTEAALQDPNSSLLLSTSSKILTLLSAPEGALPLTPGALHERKAQLSVLETGSAERIATRPTTSDSPFSTKQNKLTGSSPMGSTAENRRKKASDQFSAGSIENYFTKMRNSTPTVKKNYIFRAPRPTEKAGEEDGHSVPYGPQQNILWVEEDQPIRQLPDGKAVHGSPRTKMTLMTEKCPHERQHAGHKGLEKVVSSPRRSDPGVETDAGDDRLCFTPELYDDDDDEEVVEEKNEGMIHNHDSTLTSFETTNTVLVEELCPPSAQRPGEAGVGAEWWGAAEHSQNDRSAIEEAGHKVGIEETKQEAEREGNQLPGSQSKGLKVQRRYTRRARKTSNNRKAHQQQVTLKECVDRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBJ6</accession>
    <name>A0A6I9YBJ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-binding cassette sub-family E member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNase L inhibitor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ribonuclease 4 inhibitor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ABCE1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>CTP + H2O = CDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29387"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>UTP + H2O = UDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64900"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with PINK1. Interacts with CNOT4. Interacts with PELO. Probably heterodimerizes with RNASEL; this interaction inhibits RNASEL.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. ABCE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921492.1">
        <property type="nucleotide sequence ID" value="XM_014066017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBJ6"/>
    <dbReference type="GeneID" id="106548617"/>
    <dbReference type="KEGG" id="tsr:106548617"/>
    <dbReference type="CTD" id="6059"/>
    <dbReference type="OrthoDB" id="6593433at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03236">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03237">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000144">
        <property type="entry name" value="ATP-binding cassette sub-family E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="79"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="342"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013921492.1"/>
        </source>
    </evidence>
    <sequence length="599" mass="67342" checksum="61BBE474E9DD5457" modified="2020-10-07" version="1">MADKLTRIAIVNHDKCKPKKCRQECKKSCPVVRMGKLCIEVTSQCKIAWISETLCIGCGICIKKCPFAALSIVNLPSNLEKETTHRYCANAFKLHRLPIPRPGEVLGLVGTNGIGKSTALKILAGKQKPNLGKYDDPPDWQEILTYFRGSELQNYFTKILEDDLKAIIKPQYVDQIPKAAKGTVGAILDRKDETGTQTIVCKQLDLSHLRERNVEDLSGGELQRFACAVVCIQKADIFMFDEPSSYLDVKQRLKAAVTIRSLINPDRYIIVVEHDLSVLDYLSDFICCLYGVPSAYGVVTMPFSVREGINIFLDGYVPTENLRFRDASLVFKVAETANEEEVKKMCMYKYPGMKKKMGEFELSIVAGEFTDSEIMVMLGENGTGKTTFIRMLAGRLTPDDKSEVPVLNVSYKPQKISPKSTGSVRQLLHEKIRDAYTHPQFVTDVMKPLQIENIIDQEVQTLSGGELQRVALALCLGKPADVYLIDEPSAYLDSEQRLMAARVVKRFILHAKKTAFVVEHDFIMATYLADRVIVFDGIPSKCTVANSPQTLLAGMNKFLSQLEITFRRDPNNYRPRINKVNSIKDVEQKKSGNYFFLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBZ1</accession>
    <name>A0A6I9YBZ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hydrocephalus-inducing protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106548709</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921615.1">
        <property type="nucleotide sequence ID" value="XM_014066140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBZ1"/>
    <dbReference type="GeneID" id="106548709"/>
    <dbReference type="KEGG" id="tsr:106548709"/>
    <dbReference type="OrthoDB" id="442692at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904158">
        <property type="term" value="P:axonemal central apparatus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033305">
        <property type="entry name" value="Hydin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053879">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23053">
        <property type="entry name" value="DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23053:SF0">
        <property type="entry name" value="HYDROCEPHALUS-INDUCING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22544">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="7">
        <location>
            <begin position="442"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013921615.1"/>
        </source>
    </evidence>
    <sequence length="481" mass="53301" checksum="2C7D7D9F2370FAE3" modified="2020-10-07" version="1">MSDLGQLFTIQPQKGNLGPNDRPTQVQIVFHSKKEVKIEDKPVLQCHVIEPSLCEGGETIATIPIRLSVLSLFSKYNIVPPSIINFGPLMNSTRKTGNFTIENKGVLDFKFTICKQVRDFVITAKKGNSNIKSSRSRETDLSRQNAFMSTGRQSKHADSLQKDMNLIGQQPLKPLIVHSHFKCPSSPATFFLLLLMTKCMPNLKLDEKGPVIAVKGKSLLPYCHFELEDSDYITANRRNSDLRGPGGISLDPQTKVIEFTSVGVHVKNSSLCLIFFLLVLEWLPNKRLLPAALMMSTKPSGLLLTLLLLGQVIETDPEPTHMTVEEGSQELVLRVSANVDFAQYKMDADAVHFRDTLLFQTRVFTVELSNTGNVQLEYSWKVVMEEGGKAVNFATEPLSTSPEGDPSRASTASIKAQSSRPFSQLASLLESLSSYIFPVVTRPPFSMEPSSGKIPPGKSQSLQVKFSPQEIGEFEGRLLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCI4</accession>
    <name>A0A6I9YCI4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C9orf43 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106548827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921769.1">
        <property type="nucleotide sequence ID" value="XM_014066294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCI4"/>
    <dbReference type="GeneID" id="106548827"/>
    <dbReference type="KEGG" id="tsr:106548827"/>
    <dbReference type="OrthoDB" id="10033658at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029134">
        <property type="entry name" value="DUF4647"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36130">
        <property type="entry name" value="RIKEN CDNA 4933430I17 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36130:SF1">
        <property type="entry name" value="RIKEN CDNA 4933430I17 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15504">
        <property type="entry name" value="DUF4647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013921769.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="47111" checksum="DB6E38727BC7FB3B" modified="2020-10-07" version="1">MGIVNIGAQWDETICNMIACQHPPCWESMRRIESGHPHLLLKNVVRPGKDSPASEGKLPTLKIVDLPLHYPRREQFKCLGSFEFNSKTISSSKEARNCFSKSILNNDWIPPISVLSSEKNHFPGLNSRMESQSLHFPPISLTSSRQPEKMQVTDLSEFVVQRLGSQAPCGSTVFRWVPDMRPRFLQSGNPSGREKATPPKICVKDLALESLCSLQDHQGTKRKKSNKIPTGGQPYFRHLRRNQKSNNKSSSVGQQRSMDDSLLGTRKHPLPALHLLLQNAEGLTQESKRARALLETKEKLAAPFIVQKAPRFNLSEAKIPIKDKDPGRSLGRQPKIISGGCDSFPYCLPKANSSYRRYCEHLPNWKEATPAERDASDISREVWPKEDSQEAARTFAPLQSPGPPPPSPTLSENSPNSAPGFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCQ4</accession>
    <name>A0A6I9YCQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Synaptophysin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SYPL1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921933.1">
        <property type="nucleotide sequence ID" value="XM_014066458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCQ4"/>
    <dbReference type="GeneID" id="106548956"/>
    <dbReference type="KEGG" id="tsr:106548956"/>
    <dbReference type="CTD" id="6856"/>
    <dbReference type="OrthoDB" id="10006326at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF9">
        <property type="entry name" value="SYNAPTOPHYSIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="10"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921933.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="18773" checksum="8E0B59A74662167A" modified="2020-10-07" version="1">MSDFHLDLGPLKEPLGFIKLLEWLFAVFAFASCGGYKGVTTLLVSCHGVVNKTITASFSYPFRIMRDYIFTLCAAFLWLVSTAAWAKALVDIKISTGYRIVEEIPVCKMVDSSCTFASVTSMGSLNVSVVFGLVNMILWGGNAWFVYKETSLHKSNSNSPKPGFYPPTSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD86</accession>
    <name>A0A6I9YD86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PAPOLG</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013922107.1">
        <property type="nucleotide sequence ID" value="XM_014066632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD86"/>
    <dbReference type="GeneID" id="106549074"/>
    <dbReference type="KEGG" id="tsr:106549074"/>
    <dbReference type="CTD" id="64895"/>
    <dbReference type="OrthoDB" id="412748at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF6">
        <property type="entry name" value="POLY(A) POLYMERASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="20">
        <location>
            <begin position="38"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="19">
        <location>
            <begin position="236"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="18">
        <location>
            <begin position="383"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="18">
        <location>
            <begin position="443"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="652"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="694"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="694"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013922107.1"/>
        </source>
    </evidence>
    <sequence length="736" mass="83778" checksum="6473B84CC808F404" modified="2020-10-07" version="1">MQVLCARWRRSMRAELMLAGMKETCGTYLENQLERHYGITSPISLAPPREIDYIYSQKLIEALKPFGIFEEEEELDCRLAVLDKLNNLVKHWIAEVAENKNLPSSISSRAGGKIFTFGSYRLGVHTKGADIDALCVAPRHIERSDFFQSFFERLKLQDGIRNLRAVEDAYVPVIKFEFDGIEIDLVFARLPLSSISDSLDLRDDAHLRTLDIRCIRSLNGCRVTDEILHLVPNKENFRLTLRAIKLWAKRRGIYSNMLGFLGGVSWAMLVARTCQLYPTALASTLVHRFFLIFSKWEWPNPVLLKQPEESNLNLPVWDPRVNPADRYHLMPIITPAYPQQNSTYNVSTSTRAIMVEEFKYGLTVTDEILQGKSDWPKLLEPPNFFQKYKHYIVLTAVSSTEEHHLEWVGLVESKIRVLVGHLERNEFITLAHVNPQSFSGNRDQYKESDYVSMWFLGIIFKKLENTESVSVDLTYDIQSFTDTVYRQANNINMLKEGMKIEATHVRKKQLHFYLPADFLQKRKKSVPDLGGHAYGVMLKKTMENDSGLDTYSSAEVTTQCSSVPSFNNVVEYCSTSFSKGIERSDVFQKTTISERQEKPRTITQSAMSVPGLNLTASANTTLPLSSGSVSMAIEQNKTPPTIILDPTHSQEHLDDIGSIKNNSTHKRDHSPISEDCFKRLKDVDQHSLNNTLALPTFDTQRSQRLSSKELPDSSSPIPASGMVRVVKNSIRLTLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEJ5</accession>
    <name>A0A6I9YEJ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Syntaphilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SNPH</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922598.1">
        <property type="nucleotide sequence ID" value="XM_014067123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEJ5"/>
    <dbReference type="GeneID" id="106549464"/>
    <dbReference type="KEGG" id="tsr:106549464"/>
    <dbReference type="CTD" id="9751"/>
    <dbReference type="OrthoDB" id="5807119at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028197">
        <property type="entry name" value="Syntaphilin/Syntabulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16208">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN/SYNTAPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16208:SF1">
        <property type="entry name" value="SYNTAPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15290">
        <property type="entry name" value="Syntaphilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="225"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="131"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="424"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013922598.1"/>
        </source>
    </evidence>
    <sequence length="540" mass="58456" checksum="D7D134AF5F771585" modified="2020-10-07" version="1">MSLPGSRRPSTGSRSREFYGRSGSASHFKSATAPATPTEKQPLLPASRRPSPPVSMRDTYGTSSLSSSSNSGSCKGSDSSPTPRRPMKYLLCSDNHGIKPPTPEQYLTPLQQKEVCIRHLRARLRDTHERLQDRDGEIEDLKTQLSRMQEDWIEEECHRVEAQLALKEARKEIKQLKQVIDTVKNNLIEKDKGLQKYFVDINIQNKKLETLLHCMEVAQDGTVKEEGHAESAGGSPARSLTRSSTYTKLSDQGATDRNIGGSQTISVDDGADSGFVAAENSLSRTDLLDHSSLLSSGVECGPDDASSLHTSYTLGSQMPTSSTYEKLQSVEAAVQASCMQEQAIQTDFGHYQPDLDTILEKVMKSQAGSLASPTSEWPSEIEEVTEPTSFESGVANCSNPTDTTAPEPFSVVVVSAGEKPDGQPASQNPAVHQPCSASPSVSIVCTPEDEATDEGEDTAGAAAPAEPKTYWSRHFLVDLLAVVVPAVPTVAWLCRSQQRQGQPVYNISSLLRGCCTVALHSIRKISCRSVTNVNSSCSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFD9</accession>
    <name>A0A6I9YFD9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">von Willebrand factor A domain-containing protein 3A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106549560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922729.1">
        <property type="nucleotide sequence ID" value="XM_014067254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFD9"/>
    <dbReference type="GeneID" id="106549560"/>
    <dbReference type="KEGG" id="tsr:106549560"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46478">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46478:SF1">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="148"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013922729.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="59849" checksum="DCC3868DFCC73704" modified="2020-10-07" version="1">MEEVCKSMADARANPDSTARSPKRQKSWQDVVLTDPVDDHGLLVTHVNQTHDLMRLKDTEVMFANLKATDDWIQVYSLANLQLSLEHLMKKGTATTDAKNTVEFTEEEVNSFESQLQMAVNLYDKRMRWLLRGTKKLFGVIQGNKLGVLIETSSVDYGSGLQGLQMDLLALINEQLHYMKKLYFISFGSETSALWEEPRTVNEDRLNEARQWVQELEPTRGCNLLKALKKVLLMRELDSLAIIVGTCPDQAPEILSDYIQQCSAGRRLLVHTVAYNCGSEAPPAILKSLAEEVRGRYHCYSGKMETCNSTDIQLVLSERQKAESVLEVVNEIYEGRMGDSLFNLVTNNSAERMRSSSLVQLSKPFQHERLLAIQIPNLLAKSSAEWLKTNGLRAKKLSLYQVLAPNAFSPVEEFVPVLRKTVSSTLHGKAMMQFEWHDGTMKNIHVDPPILYDYQKQLRQTVKMYERRIDWLSLSSRRIWGTVCENRYQDWTSIQFKCHLAFYFYIYLFLLSNPFSILHTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFF8</accession>
    <name>A0A6I9YFF8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">F-box only protein 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106549764</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922973.1">
        <property type="nucleotide sequence ID" value="XM_014067498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFF8"/>
    <dbReference type="GeneID" id="106549764"/>
    <dbReference type="KEGG" id="tsr:106549764"/>
    <dbReference type="OrthoDB" id="1107553at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22168">
        <property type="entry name" value="F-box_FBXO6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000012">
        <property type="entry name" value="F-box only protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000002">
        <property type="entry name" value="F-box only protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007397">
        <property type="entry name" value="F-box-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039752">
        <property type="entry name" value="F-box_only"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125:SF12">
        <property type="entry name" value="F-BOX ONLY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125">
        <property type="entry name" value="F-BOX ONLY PROTEIN 6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04300">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01198">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51114">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="FBA" evidence="3">
        <location>
            <begin position="69"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013922973.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="29538" checksum="0AA4D64DAD746B96" modified="2020-10-07" version="1">MASMENLPEDALIAVLVLVPAQDLVRHCRLVCSLWRGLVDLPSLWKLKCQREGYWPEPLDTPIPDWRNFYFLCSLKRNLIKNPCAEEGFEAWKKESNGGNGWKIEDLPGDHGRNFLLPHVQKYFVTSYDSCMKGQMITLRDHGYWDRLMDEARPDIVVSDWFAARFDCGCQYELCVRLLSKDYIVLQEFVLEPVVIEQWSDAEWRKVSHTFHNYPAGVRHILFSHGGHDTQYWEGWYGIRVTNSSLTLGPQVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGJ1</accession>
    <name>A0A6I9YGJ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PFKFB4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923297.1">
        <property type="nucleotide sequence ID" value="XM_014067822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGJ1"/>
    <dbReference type="GeneID" id="106550043"/>
    <dbReference type="KEGG" id="tsr:106550043"/>
    <dbReference type="CTD" id="5210"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000001">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000047">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF14">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="9">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="258"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013923297.1"/>
        </source>
    </evidence>
    <sequence length="471" mass="54337" checksum="C58682CE7B4E0666" modified="2020-10-07" version="1">MASSPARELTQNPLQKIWVPYSNGLPAKHSAQRGVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTKEFNVGQYRRDLVKTYKSFEFFLPDNKEALKIRKKCALAALNDVRRYLCEENGHVAVFDATNTTRERRDTIYKFGEENGFKTFFVESVCVDPEVIAANIVQVKLGSPDYVDCASDEATEDFMKRIECYKNSYETLDENLDKDLSYIKIMDVGRSYLVNHVMDHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDTGLSHRGKEFAKSLGQFINDQNIKDLKVWTSQMKRTIQTAEALRVPYEQWKVLNEIDAGVCEEMTYEEIQENYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLIAYFLDKPAEQLPYLKCPLHTVLKLTPVAYGCKVESIFLNVEAVNTHRDKPQNVDIVRPPEDALVTVPAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH20</accession>
    <name>A0A6I9YH20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">EIF4A3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013923477.1">
        <property type="nucleotide sequence ID" value="XM_014068002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH20"/>
    <dbReference type="GeneID" id="106550173"/>
    <dbReference type="KEGG" id="tsr:106550173"/>
    <dbReference type="CTD" id="9775"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18045">
        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000498">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="47"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="228"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="15">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013923477.1"/>
        </source>
    </evidence>
    <sequence length="389" mass="44520" checksum="6A67FBE5B716E984" modified="2020-10-07" version="1">MTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIJ2</accession>
    <name>A0A6I9YIJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorting nexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SNX9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924032.1">
        <property type="nucleotide sequence ID" value="XM_014068557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIJ2"/>
    <dbReference type="GeneID" id="106550621"/>
    <dbReference type="KEGG" id="tsr:106550621"/>
    <dbReference type="CTD" id="51429"/>
    <dbReference type="OrthoDB" id="10254720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07668">
        <property type="entry name" value="BAR_SNX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07285">
        <property type="entry name" value="PX_SNX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11898">
        <property type="entry name" value="SH3_SNX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000004">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037425">
        <property type="entry name" value="SNX9_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014536">
        <property type="entry name" value="Snx9_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037426">
        <property type="entry name" value="SNX9_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035558">
        <property type="entry name" value="SNX9_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019497">
        <property type="entry name" value="Sorting_nexin_WASP-bd-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827:SF2">
        <property type="entry name" value="SORTING NEXIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10456">
        <property type="entry name" value="BAR_3_WASP_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027744">
        <property type="entry name" value="Snx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="242"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="85"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="143"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR027744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR027744-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924032.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="66260" checksum="266FFC885AD69603" modified="2020-10-07" version="1">MATKARVIYDFAAEPGNNELTVNEGEIITITNPDVGGGWLEGKNSQGDRGLVPTDYVEIIQESTRDGILVADQAFFDSFSSNASQTNSQAVKNNSQVTSNANDPWSSWENTTADDAWSTKPEARQKNSASNNWDAAAFGHPQAYQGPAAADDDEWDEEWDEPKSAATSYPGYKEAEPSDPAGVQRGNSRGTAMKLPLNKFPGFAKPGVEQYLLAKQLVKPKEKIPIIIGDYGPMWVYPTSTFDCIVADPKKGSKMYGLKSYIEYQLTCTNTNRSVNHRYKHFDWLYERLLIKFGTAIPIPSLPDKQVTGRFEEEFIKMRMERLQAWLTRMCRHPVISESDVFQQFLSFRDEKEWKTGKRKAEKDEIVGVMVFSTMEPEAPDMDMIEIEQKCDAVGRFTKSMDDGVKELLTVGHEHWKRCTGPLPKEYQKIGKALQGLALVFSTSGYQGESDLNEAITEAGKTYEEIASLVAEQPKKDLHFLMETNHEYKGFLGCFPDIIGAHKGAIEKVKESDKLIATSKITPQDKQNMVTRASAMSYALQAEMNHFHSNRIYDYNTVMRLYLEQQAQFYETIAQKLRQALSRFPMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIR2</accession>
    <name>A0A6I9YIR2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Transcription factor HIVEP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">HIVEP2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924091.1">
        <property type="nucleotide sequence ID" value="XM_014068616.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924092.1">
        <property type="nucleotide sequence ID" value="XM_014068617.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550672"/>
    <dbReference type="KEGG" id="tsr:106550672"/>
    <dbReference type="CTD" id="3097"/>
    <dbReference type="OrthoDB" id="10042249at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000033">
        <property type="entry name" value="Immunodeficiency virus type I enhancer binding protein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000594">
        <property type="entry name" value="Transcription factor HIVEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051969">
        <property type="entry name" value="Zinc-finger_DNA-bd_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944">
        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR HIVEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="1780"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="1808"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="369"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="703"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="887"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1085"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1467"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1868"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2013"/>
            <end position="2116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2344"/>
            <end position="2367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2380"/>
            <end position="2417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="716"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="887"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="947"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1085"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1136"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1493"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1529"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1557"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1879"/>
            <end position="1905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1908"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="2013"/>
            <end position="2034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2059"/>
            <end position="2089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2105"/>
            <end position="2114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2397"/>
            <end position="2417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013924092.1"/>
        </source>
    </evidence>
    <sequence length="2417" mass="268675" checksum="D759B89A72124E31" modified="2020-10-07" version="1">MDSGDIAIGQNATSRTGEAAKVSSRWRQEHSANVKMGSLEGQAQIDPEHMGSPVSSQLFSSGKGVSPSNATQQITDKQYPQQLPSPYSCPHSLSFPQPSLPQGFLHNLKPRQSLEGHKWQFSGPLQPVASEDLFPFHVHGHSGSFSRKKVSNLNPAYSQYSQKCIEQSSEDGYKKEHKSKKPGKYICPYCSRACAKPSVLKKHIRSHTGERPYPCVPCGFSFKTKSNLYKHRKSHAHAIKAGLVPFTESAVSKLDLDSSFIDVEAEIHSDGEQSTDTDEEASLLVESSEKLSPVPQIPLDITSRSGFHASLEESLGSPMKVPILIIPRSGVPLPCESSLHVGTDNLQNPLNSKSDDSHTVKQRLALRLSEKKGQDSDQSLNLLSPHSKGSTDSGYFSRSESAEQQISPPNTNVKSYEEIIFGKFYRINQRTAQPLATANQDCNSVGRKGKMEPLPLVNPRLDINLSEERMSPNNEALMDSGNRNIMKNSQVFDDCSSESKQLQIVTSSIKNESKLYPGSHLGDVSLLLEPSADSNPLIRSNSMPISSATNLNVPSSLRGSHSFDERMTGSDDVFYPETIGIPHQRMLIRQAAFELPSLQEGHSDSEYHGRMGKNIMVASIRSPIGEKGPGMREKKLPAGERHIYEGDSSGKHYRKWEDYGILKQSFSGSKQSREFYTESPTQLQAMSSTYGIVFENRKRRIQKSVGDEEDMPSLCSGVSSNSMTGDFDPKSQTQDGPRSGYGIQSHESAAHCHSERFEMCRPHLQSCSPTTSLEECSSAADQEKAAESLSRKGNVISVIQHTNSLSRPNSFERSESTELATCQQEKSYLTSESFESEVAEFPMSPERVQQIENAEVASKQPSSLQPFQTYMQPRLVRQHNIQVPEIRVTEEPDKPEKEREVPVKEQEKPAEEFQWPQRSETLSQLPAEKLPPKKKRLRLADMEHSSGESSFESTGTSLSRSPSQESNLSHSSSFSMSFEREDSIRFMTSSRQDELAKQPEFLTVPAGAYSLSVPGHHQQKEMRRCSSEQMPCPYPAEIPEIRSKSFDYGNLSHASAAGAPPVALSPVRERKKCFLVRQASFSGSPEISQADSSMEQNIKQEQTEHGHAGLRSSQTPWSHSSFSLPSDDSRKQATGSCPSRSSSLSPLSHLSVPQAPYILSKYPAEQQHLFALQEKVPFFPIHSTILQFPYPSVCMVHLPSQPLWPSEISQTPFPHHFVQQLQKSYSKQPFPMESHPGCHLEYAQEHIRKKADYICTKEQPYQRYSGTSGQYSKNALAIYQPDCSIKSTDTSSEQQLQVNFETQNIEPVQSLPGTVVPVRLQTHVPSYGSVMYTSISQILAQSSSPAIVICKVDETLSQRTLATSSCMHGIEFNIAQMLNQHGGLEKYPLWKVPQTLSLNLDSSIPLCLTPSSDTASSLGGNKRMLSPASSLELFMETKQQKRVKDEKMYGQIVEELSAVELTNSDIKKSFPRPQKPQLVRQSCTTESKERGHSPLLCSPLSSSSQDLPGSKPSPAESSPLNKEKLPSFDSASLGQKSSGTSEGRESPEELDVDETSSDMCLSPQRSLVSSGESQMEDQSKPQKLPFGMLVQMASQPSGKAVTSTILLTDLADIQHILKFPSLRTTTTVSWCFLNYTKPSFIQQATLKSSVYASWCISSCNPNPSGLSTKITLALLRSKQKSSTEIYTLSPMHRPGMGKLTSSSTWKEFAQMKQEPFFVFGSKFEKKVAGNVIKERIKGDSHVDKDIVVKQTEPMRIKIFEGGYKSNEDYVYVRGRGRGKYICEECGIRCKKPSMLKKHIRTHTDVRPYICKLCNFAFKTKGNLTKHMKSKAHMKKCLELGVSMTSVDDTETEEAENMEDNPQEIEKNMTGTSAQHQFSDAEESDGEDGEDNDEDDEDEDDFDDALGDSTPKTRSRSTSPQPPQFSSLSVNTSTTSYGGSPESAVGHSSLISYLVTLPSIQVTHLLSTSDSCDDSPMTEYQRFFQNKSTDSEPDKDRLDIPSCMDEDYMISLESNSSPRDLSSSSQQSSPGYDSSPYRDNSPKRYLIPKGDLSPRRHLSPRREISPIRHISPRKEAVLRRELSPRRDASPRRHLSPRRPVSPGKDASSRREMSPRRERRYMASIRAASPRRGLYHNPSLPLGQYMQSESLSLGHSNSRAGMLEPPYFNLPGEKGILEHHIPNLFPEGPTDYVFSHLPLHSQQQLRAPIPMMPIGGIQIVHSLPAALSGLHTPSIWSLSKESFEEKQWESCTPPKHLENCARPPSLHMMAQDSTSTSELLLMKKSTLEEGGVMEREQEENIQTCTKAIASLRIATEEILHGTEQTERDSKPFRKPLESAHFSMEHFSGSEPGQICASATSPDLHSGEKDLFGTSQQTVVSHSPFYSKSSVEVRPSVYPSRKDSSTQESREPLSEKSKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJL0</accession>
    <name>A0A6I9YJL0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uroplakin-3b-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923965.1">
        <property type="nucleotide sequence ID" value="XM_014068490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJL0"/>
    <dbReference type="GeneID" id="106550555"/>
    <dbReference type="KEGG" id="tsr:106550555"/>
    <dbReference type="OrthoDB" id="9939598at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024831">
        <property type="entry name" value="Uroplakin-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15446">
        <property type="entry name" value="UROPLAKIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15446:SF2">
        <property type="entry name" value="UROPLAKIN-3B-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923965.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="26372" checksum="9F308E853C19BA65" modified="2020-10-07" version="1">MVTAGCTSQPPNFSPKLVNYVPHLTSEEMEGKITTSTFALQQPRCVFNKLVNATDVIWLVVALSDAIKSFKAPKTPEALPYQSFRKNYFYMTLNTTVSNYPCPEKSNDITILRVGSETECVVDPSRPDCNGPLAGPGPYRVRFLAMNSTGVIIKESKWSDPIRLIQGRNPDRIVVKPRRRRTETNVIISILSILSAILLAALIAAFIYKYSSICDKPMSADFVPREDPTVVRYIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YND9</accession>
    <name>A0A6I9YND9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">UDP-N-acetylhexosamine pyrophosphorylase</fullName>
            <ecNumber evidence="6">2.7.7.23</ecNumber>
            <ecNumber evidence="17">2.7.7.83</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-pyrophosphorylation enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">UDP-N-acetylgalactosamine pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">UAP1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Isoform AGX1 has 2 to 3 times higher activity towards galactosamine 1-phosphate (GalNAc-1-P).</text>
    </comment>
    <comment type="function">
        <text evidence="16">Isoform AGX2 has 8 times more activity towards glucosamine 1-phosphate (GlcNAc-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + O-phospho-L-seryl-[protein] = O-diphospho-L-seryl-[protein] + 1D-myo-inositol hexakisphosphate</text>
            <dbReference type="Rhea" id="RHEA:64104"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="Rhea" id="RHEA-COMP:16509"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:149682"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:64105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-acetyl-alpha-D-galactosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-galactosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34363"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:61970"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.7.7.83"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:34364"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13510"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="6"/>
    <dbReference type="EC" id="2.7.7.83" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013925983.1">
        <property type="nucleotide sequence ID" value="XM_014070508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YND9"/>
    <dbReference type="GeneID" id="106552281"/>
    <dbReference type="KEGG" id="tsr:106552281"/>
    <dbReference type="CTD" id="6675"/>
    <dbReference type="OrthoDB" id="532420at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052630">
        <property type="term" value="F:UDP-N-acetylgalactosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04193">
        <property type="entry name" value="UDPGlcNAc_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.100:FF:000001">
        <property type="entry name" value="UDP-N-acetylhexosamine pyrophosphorylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000043">
        <property type="entry name" value="UDP-N-acetylhexosamine pyrophosphorylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF4">
        <property type="entry name" value="UDP-N-ACETYLHEXOSAMINE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00072936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013925983.1"/>
        </source>
    </evidence>
    <sequence length="524" mass="58956" checksum="B1EE304637CCCC42" modified="2020-10-07" version="1">MDSDINILQRCLAEAGQSHLLQFWEELNELEKQELYKELNTMDLKELDQAFKKAMLDSSRLPGQESMDAMMEPVPREVLGSTTRDQDQLLIWEKEGLRQISQSKVAVLLLAGGQGTRLGVSYPKGMYDVGLLSHKTLFQIQAERILKLQQLAEKQYGLQCVIPWYIMTSGRTIKLTEEFFLKHKYFGLKKENVIFFKQGMLPAMDFNGKIFLEEKGKISMAPDGNGGLYRALGTHHIIEDMEQRGIGSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVYKVVEYSEISLATAQKRSSDGRLLFNAGNIANHYFTMEFLKDIVNNYEPQLQHHIAQKKVPHVDVTTGKALKPDKPNGIKMEKFVFDIFQFAKNFVVYEVLRENEFSPLKNADSQNGKDNPTTARHSLMSLHHCWVLNAGGHFVDENGTRLPAIPRDRNGRSDVIQADAKPNLKDANDSPIQCEISPLVSYGGEGLVELVKDKEFHAPLIIDQSGIHHLVKNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNF7</accession>
    <name>A0A6I9YNF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA ligase 1</fullName>
            <ecNumber evidence="2">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA ligase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106552132</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013925797.1">
        <property type="nucleotide sequence ID" value="XM_014070322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNF7"/>
    <dbReference type="GeneID" id="106552132"/>
    <dbReference type="KEGG" id="tsr:106552132"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000001">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA ligase ATP-dependent N-terminal" evidence="16">
        <location>
            <begin position="281"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="15">
        <location>
            <begin position="535"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="55"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="141"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013925797.1"/>
        </source>
    </evidence>
    <sequence length="629" mass="69508" checksum="47A4D8D7FAA25B95" modified="2020-10-07" version="1">MQSNIRSFFQPLARVKSEKEKTEDSNLEDVLSSQKKLVNDSELCEDFTVKCASKKPKVLKSDDEDDPVELDGKDAQADDNKLSPDCVSEEKISEESISPSISPITSNSATSVSIPRRKTARKQLSKRKPEEDDNSYSYENHSSKRWKENLDGDDSKDKTNEKLIEVEEVKSENATLVEHKTDKNAKEEEIPDSSKPSSKSISSFFAPKKSSVKIEKKQAPVVSASISVPKCFNKISNDETSSIADNFLKPSLTFELAEYNPTKSGYHPVLDACWVPGQKMPYLAVARTFEYIEKESARLKIIETLSNLFRSAIVLSPQDLLPCVYLCLNQLGPAYEGLELGIGKTILMRALAQATGRQLDQIKAEAAEKGDLGVVAENSRTTQGTVIAPLKLGAAAVFGKLRAIGQMVGSSSMNKKIDIIKSLFVACRHSEARYVARSLQGKLRIGLAEQSVLSALAQAASLTPAGQKFPPKVLDAAKGKSADARKAWLDERVLIVKQAFCELPNYDIIVPVLLEHGVESLPQYCKITPGIPLKPMLAHPIKGIGEVLKRFEEAAFTCEYKYDGEREQIHILENGEVHIYSRSQENTTTKYPDIVSRVPKVKNSTVKSCILDAEVVAWDPVSKQILPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQM4</accession>
    <name>A0A6I9YQM4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Chondroadherin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CHAD</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class IV subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926466.1">
        <property type="nucleotide sequence ID" value="XM_014070991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQM4"/>
    <dbReference type="GeneID" id="106552663"/>
    <dbReference type="KEGG" id="tsr:106552663"/>
    <dbReference type="CTD" id="1101"/>
    <dbReference type="OrthoDB" id="2190652at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000059">
        <property type="entry name" value="Chondroadherin like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617:SF101">
        <property type="entry name" value="LEUCINE-RICH ALPHA-2-GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026925452" evidence="10">
        <location>
            <begin position="24"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="11">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="12">
        <location>
            <begin position="302"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013926466.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="40601" checksum="DF8A0751E04BC238" modified="2020-10-07" version="1">MDPSNILLKSIALLICLLPVLQACPQNCHCHGGDLHHVICDNVGLRRIPKVAEQTRLLNLQRNNFPVLPTNGFREMKNLVSLHLQHSHIKEISSGAFRGLKQLVYLYLSNNDISTIKMGAFDDLAELTYLYLDHNKVSDLPTGLLSPLINLFILQLGNNRLRELRSGAFHGTKNLRWLYLSNNSISSIQPGALDDVENLAIFHLDKNQLSSYPVSAMSKLRVVEDLKLSNNPIKSIPDLAFQSFGRYMETLSLDNMGLEKFSDKAFVGTTALKNVHIEHNKISSLPRSFPFTRLETLSLSNNPWYCSCQLAPLRRWLGSTRTRPDAVCASPPQTRGQQIRDTAALRSCKLPSKRSKKGNRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRD9</accession>
    <name>A0A6I9YRD9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nck-associated protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NCKAP5L</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926872.1">
        <property type="nucleotide sequence ID" value="XM_014071397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRD9"/>
    <dbReference type="GeneID" id="106552989"/>
    <dbReference type="KEGG" id="tsr:106552989"/>
    <dbReference type="CTD" id="57701"/>
    <dbReference type="OrthoDB" id="8930856at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032769">
        <property type="entry name" value="NCKAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026163">
        <property type="entry name" value="Nckap5l"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21740">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21740:SF3">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15246">
        <property type="entry name" value="NCKAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nck-associated protein 5 C-terminal" evidence="3">
        <location>
            <begin position="709"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="494"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="957"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1086"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="558"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="607"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="632"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="660"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="670"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="733"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="760"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="962"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1096"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1134"/>
            <end position="1155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013926872.1"/>
        </source>
    </evidence>
    <sequence length="1155" mass="124298" checksum="8E94BAED3A4A364A" modified="2020-10-07" version="1">MSEIEPQSEMKPEGDGHEPRTPQDLLQRLRELEAENLALVQANENQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNQALSDLFHRKLTSGSLSQTPAHPASLISSPSPISQPGTVENLPVSFPLGRCLLQREVISEEHCLEIPGSNPQSLEALSPFLRKKAQILEVLRSLEEMDPLLAFPSPWREPGQLSSPEGVTAEAWPCLLLAQGEGGPRQPPLKSEGSSSSSSDEAGEPDPPEKGHPGQVLLSALAEHKLELGEVETYLQHVLREAGDSALLNGEPSGPSLISNGQGRSYKHIQAVSCLEALGPPSACREPAYLSVLASTEDKDKSRPGLPIPSPSKVLKLLKMPPPPSPAALRLSPQLTRSSKIPCRSNTYEVYHSPTPSHKGSPDSPFPADSGIGGGHPSPKAGRPLVPESPTSAPTLPDPFGYHKPHEYENILELTLSTAMSSRSPSFRDTKLDAPEALLPSCRLAPLRVVAPGDVSLEPCPAGHREKSGSESRRSPPLARRNPDNSKRGASGSTKRALEVAASMPFKERLTVLGKLKGAEGKEGPGFEKGRAPTRPGERGESFPDAHPRLGSGSSSLKHPEPCHGNESTPRCYSSHSVGSRGNDAEHPGSKSRSLGTPVNRNPSKPLLPGKNAKSPHGSPTKLPSKSPTKPPAPEPKPCPPTAKASSGFGRKNPPGPDQAKGKAGPPASIEEKVMKGIEENVLRRHKGGPTSGETKQKNSSGLASWFGLRKSKLPALSRRPETPKAKDSKSGSRKQESESLNISKLMEKAEDLRKALEEEKAYINGLALDKTRSHTCDTGHSQLTLMYQEVTAENFMQQLLNRVDGKEAPCETHLEQKNELQGLQRGLRDAKDPRLIRPPRNGIVGHLHACPEAPDKLHEAALREDLPSDDSLAELVTSQHFAVCSSLTRTLDSGIGTFPPPDYCSGMPCKSVPKLRPRLDPPPAGPLPPRGPPPVTRVPRKARTLEREVPSAEDLMGPSKHQSMPAFHGILTSAEPALSRHDRRGCLQDLCSEASNPRRIQHGKNWTFPNAKASAGMDPFLCSSDRLEEFHGSAMGSACNVVERRRASSEGPQASPAFSTSSSRTPSASDVGEEGSTEACSRDMGPEGQPGLENSESLSDSLYDSLSSCGSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS38</accession>
    <name>A0A6I9YS38_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reelin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106552880</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Extracellular matrix serine protease secreted by pioneer neurons that plays a role in layering of neurons in the cerebral cortex and cerebellum by coordinating cell positioning during neurodevelopment. Regulates microtubule function in neurons and neuronal migration. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Oligomer of disulfide-linked homodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the reelin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926749.1">
        <property type="nucleotide sequence ID" value="XM_014071274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS38"/>
    <dbReference type="GeneID" id="106552880"/>
    <dbReference type="KEGG" id="tsr:106552880"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070325">
        <property type="term" value="F:lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034968">
        <property type="entry name" value="Reelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049419">
        <property type="entry name" value="Reelin_subrepeat-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11841">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11841:SF1">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21471">
        <property type="entry name" value="Reelin_subrepeat-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013926749.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="15202" checksum="7E23D04E0128688E" modified="2020-10-07" version="1">MFVLQIGSLSQTDSCNTNLSDPNTVDKAVLLQYSVNNGITWQVIAQHQPKDFIQAQRVSYNVPLEARMKGVLLRWWQSRHCGSGHDQWALDHVEVVHTRKQNYMMNFSRQHGLRHFYNRRRRSLLRRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTB0</accession>
    <name>A0A6I9YTB0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Brevican core protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106553351</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927296.1">
        <property type="nucleotide sequence ID" value="XM_014071821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTB0"/>
    <dbReference type="GeneID" id="106553351"/>
    <dbReference type="KEGG" id="tsr:106553351"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF41">
        <property type="entry name" value="BREVICAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="125"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="12">
        <location>
            <begin position="174"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="13">
        <location>
            <begin position="292"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="294"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927296.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="38794" checksum="0BE25164D86477FE" modified="2020-10-07" version="1">MTPAGSPEESGDHSGSSWPPPARLSPVAVSSPPDEAAAAASKASAGLDESKEEPWAEASEEEQEPTLRTTVPAADKGGHPEAVPSGGPLGLNFFSTPFLEASLTTTHPRPVLPTESASLGASGSVSDTCVPNPCRNGGTCTEGRGRPGCLCLPGYGGDACNVSLRKCQPGWDAFQGFCYKHFSARRSWEDAETRCREHGGHLANIMSPEEQSFINNQYKEYQWIGLNDRTIEGDFQWSDGSPLLYENWHNGQPDSHFLYGENCVGLSWRKDGKWSDVPCGYHLPFTCKMGLISCSSPPKVADAQIFGKPKQRYEVNSVLRYWCPEGFLQHRWPLIRCQENGQWQQPQFACSPPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTQ6</accession>
    <name>A0A6I9YTQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Amiloride-sensitive sodium channel subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SCNN1B</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927431.1">
        <property type="nucleotide sequence ID" value="XM_014071956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTQ6"/>
    <dbReference type="GeneID" id="106553453"/>
    <dbReference type="KEGG" id="tsr:106553453"/>
    <dbReference type="CTD" id="6338"/>
    <dbReference type="OrthoDB" id="6502088at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034706">
        <property type="term" value="C:sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000002">
        <property type="entry name" value="Amiloride-sensitive sodium channel subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004724">
        <property type="entry name" value="ENaC_chordates"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00859">
        <property type="entry name" value="ENaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF18">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="630"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="671"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013927431.1"/>
        </source>
    </evidence>
    <sequence length="688" mass="77431" checksum="68DCFC8E61620BE8" modified="2020-10-07" version="1">MTLKKYFVKALHRLQKGPGYTYQELLVWYCDNTNTHGPKRIICEGPKKKLIWFFLTLLFASLVFWQWGILIKTYLSYNVTASLAIGFKTMTFPAVTICNASPFRYSKVKPFLKDLDELIEAALERILQPLQENASATPAANLSEKLNLQLWNQMPLVLIDESDAAHPVIHDILGNSSLALGGHHNYSSFGNPEARRYKLALKLCTHLTPHCFYRKFTSAAQAMAEWYVLQSTSILSKVPLSKRIEMGYQAEDMILACLYGAEPCNHRNFTHLYHPDHGNCYVFNWGVEKQPLVSSNPGAEFGLKLILDISQKDYIPFLTTTAGARLMLHEQRSFPFLKDQGIYAMSGTETSIGVLVDELERMGSPYSDCTVNGSDVPVRNLYEDYTASQAKEMGACANCTHPGSKMAPFQSQYKTMYSIQACLRSCFQAKMMKICQCAHYSYPLPKGAHYCNNEDYPNWAYCYSILRMSQEHRQMCIQSCKETCNNTQYKMTISMADWPSEASEDWIFHILSYERDTSTKVTLDRNGIIKLSIYFQEYNYRTISESAATTIVWLLSSLGGQFGFWMGGSVLCLIEFGEILIDFVWISVLKLISWGKGLKWKPAPRPPDALPSVCQKVEAYTNLGFCGEEEKEAAAAEDPGDGPPDAPGAGSEPGTPPPKYDSLRVLPLDLLEPESDEEDAPGVDTKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU94</accession>
    <name>A0A6I9YU94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease H</fullName>
            <ecNumber evidence="4">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">STOML2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013927877.1">
        <property type="nucleotide sequence ID" value="XM_014072402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU94"/>
    <dbReference type="GeneID" id="106553829"/>
    <dbReference type="KEGG" id="tsr:106553829"/>
    <dbReference type="CTD" id="30968"/>
    <dbReference type="OrthoDB" id="434619at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000003">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000026">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032435">
        <property type="entry name" value="STML2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16200">
        <property type="entry name" value="Band_7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="176"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927877.1"/>
        </source>
    </evidence>
    <sequence length="517" mass="57977" checksum="4CC32809B88067C4" modified="2020-10-07" version="1">MPFGLQGVPAVFMQLINEVLHDHLYKGVIVYLDDILIYTETREEHVRLVCTVLKKLRAAELYAKLSKCEFHQEKVDYLGYCISHRGIEMDPAKVKAVTEWEAPRTCKQLQKFWGFANFYRQFIPSFAKIALPITNLLKSKGGAKPKPSKPLDWTMDCQAAFEKLKRLFAEESVLKHPDMDAPFVVQADASDVAVGAVLLQANSQGLSSSHYPQTNGACERTNSVLEQYLRCFINYQQDDWVDLLPHAEVAYNNSVHSSTGFTPFRVMYGQDFVPIPEIPQEQPQVSSLSEWSDRLRGVWPLALQTLDAAHRTHKKQADKKRTKPYEYKERESLNASIVYAINQASDYWGIRCLRYEIKDIHVPPRVKESMQMQVEAERRKRATVLESEGIRESAINVAEGNKQAQILASEAEKTERINKAAGDASAILVKAQAKSEAIGLLSMALTQQNGNMAASFSVAEQYVQAFSQLAKESNTILLPTSTGDVTSMVTQAMSIYSSLNKTQAPPPESLEEPPASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVT2</accession>
    <name>A0A6I9YVT2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Apoptosis-stimulating of p53 protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PPP1R13B</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ASPP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928392.1">
        <property type="nucleotide sequence ID" value="XM_014072917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVT2"/>
    <dbReference type="GeneID" id="106554274"/>
    <dbReference type="KEGG" id="tsr:106554274"/>
    <dbReference type="CTD" id="23368"/>
    <dbReference type="OrthoDB" id="10038642at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17224">
        <property type="entry name" value="RA_ASPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000008">
        <property type="entry name" value="Apoptosis-stimulating of p53 protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000030">
        <property type="entry name" value="Apoptosis-stimulating of p53 protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047163">
        <property type="entry name" value="ASPP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028319">
        <property type="entry name" value="ASPP1_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048942">
        <property type="entry name" value="ASPP2-like_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131">
        <property type="entry name" value="APOPTOSIS-STIMULATING OF P53 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131:SF5">
        <property type="entry name" value="APOPTOSIS-STIMULATING OF P53 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21801">
        <property type="entry name" value="ASPP2-like_RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027000249" evidence="15">
        <location>
            <begin position="17"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="944"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="977"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1043"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="478"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="641"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="725"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="790"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="840"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="880"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="149"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="293"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="533"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="660"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="679"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="852"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013928392.1"/>
        </source>
    </evidence>
    <sequence length="1114" mass="124018" checksum="6D86ECC1331C357C" modified="2020-10-07" version="1">MSWYFFLLLLLLPVLPLREEMTAPEMMPMILTVFLSNNEQILTEVPITPETTCRDVVEFCKEPGEGSCHLSEVWRGNERHIPFDHMMYDHLQKWGPRREEVKFFLRHEESPTESNEQGRQTQNHRNGISIPVEKRTENGVGNPRVELTLSELQDMAARQQQQIENQQQMLVAKEQRLRYLKQQERRQQQSVSESEKLQKLKERVETQETKLKKIRAMRGQVDYSKIMNGNLSTEIEHISAMFQEKQQELQAAVLKVDQLTQQLEDLRKGKLNGFQSYNGQMTGPAAIELKKLYQELQIRNRLNQEQNTKLQQQKELLNKRNMEVAMMDKRINELRERLYKKKVEARQKENIPLNRINGSSSPQSSLNASGRVAAVGPYIQVPSSNNYAVPLDPVKPQSLTITASTTHGRSKSANDGTWPALKQTAVVKPPQLSNTDWKESNMDASLKQGTISSQPLSSSGLGSTDKLSLDVGKIPPTIPGVNKQLPQNYGTYPSSVPSGPGSTNSLERRKDGSLPRPSSTAVNRQRPIPLPPTSNIHQPGSSQQIQQRISVPPSPTYQPSNAPLFPGGDSRPDLPLTVAIRPFLAEKGSRPQSPRKGPQTVNSSSIYSVYLQQATPPKNYQQAVYSTLNKSVKAVYGKPVVQSGSTSPSPLPFLQGPLPASTSQSQAPNEMSEKEQEQESTPSSGDNSNVENIPRPLSPTKLTPIVHSPLRYQSDADLEALRRKLANAPRPLKKRSSITEPEGPSGPNIQKLLYQRFNTLAGGIESAPFYESSNSQEFIGTLADVDNGNMNSNGNIEAPVSAPSTVPLSDEPSSDANDNELPSPATEELISVETTNQIPETIEDDNNNNPAMAPPIERPPSPTPMGSSPDDDEVQVLPVLPPAPPLPVTKRTNLKKPDSERTGHTLRVKFNPLALLLDASLEGEFDLVQRIIYEVDDPSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPLHCAASCNSVHLCKLLVESGAAIFASTISDIETAADKCEEMEEGYIQCSQFLYGVQEKLGVMNKCIVYALWDYEAQNNDELSFHEGDALTILRRKDDNETDWWWARLNDKEGYVPKNLLGLYPRIKPRQRTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YW32</accession>
    <name>A0A6I9YW32_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MAM domain-containing glycosylphosphatidylinositol anchor protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MDGA1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928848.1">
        <property type="nucleotide sequence ID" value="XM_014073373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YW32"/>
    <dbReference type="GeneID" id="106554655"/>
    <dbReference type="KEGG" id="tsr:106554655"/>
    <dbReference type="CTD" id="266727"/>
    <dbReference type="OrthoDB" id="6107927at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008046">
        <property type="term" value="F:axon guidance receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000240">
        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000262">
        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000303">
        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000243">
        <property type="entry name" value="MAM domain-containing glycosylphosphatidylinositol anchor protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050958">
        <property type="entry name" value="Cell_Adh-Cytoskel_Orgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080">
        <property type="entry name" value="CONTACTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080:SF32">
        <property type="entry name" value="MAM DOMAIN CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026718876" evidence="2">
        <location>
            <begin position="19"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="24"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="132"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="240"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="338"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="439"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="538"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="636"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013928848.1"/>
        </source>
    </evidence>
    <sequence length="743" mass="83395" checksum="2FCABED7F48DC74B" modified="2020-10-07" version="1">METLWLLFLSLVPVYIRGQGVYAPAQAQIIHAGQACVVKEDNISERVYTIREGDILVLQCLVTGHPRPQVRWTKTAGSASDKFQETSVLNETLLIEKIQRLQGGRYYCKAENGVGVPAIKSIRVDVQYLDKPVLTVHQTISDVRGSFYHEKTVFLRCTVSSNPPARFIWRRGSETLSHSQDNGVDIYEPLYTEGETKVLKLKNLRPHDYASYTCQVSVRNVCGIPDKTVTFELTNTTAPPALKLSVNETLVVNPGDNVTIYCSLTGGDPLPQVVWSHSPGPLPLNSLIQGGNLTIWGIQTEDSGYYNCTAINNVGNPAKKTVNLLVRSMKNVTFQITPDVIKESETIQLGQDLKLSCHVDAVPQEKVFYFWYKNGKLAKFSDRVVVIKNDPELPPVTSSLEIIDLRFTDYGTYVCVASFQGAPIPDLSVEVNISSETVPPTIMVPKGQSIITVREDSRAELQCEVRGKPKPPIIWSRVDKEAPMPSGTMTVETNDGKLYFEKVTQKMSGIYKCQTARYNGFNIRPREALVQLNVQFSPVVEPVFQDIRQAMGRSVTLRCTMLKGSPMKVATAVWRFNGSLLTVPPTEQQDYSEYKVDTVSRETSGNYECSISNDVGVAVCKFQVSAKAYSPEFYYDTPNPTRKQSKNYSYVLQWTQKEPDAVDPILSYRLDVRQLNHRDLPPKSISVRKMEKGMLQEYLLTDLKVPQSYELRLTPITRFGMGDIVTRIIHYIEPISYPNPVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXF5</accession>
    <name>A0A6I9YXF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calponin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CNN3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6">Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929338.1">
        <property type="nucleotide sequence ID" value="XM_014073863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXF5"/>
    <dbReference type="GeneID" id="106555091"/>
    <dbReference type="KEGG" id="tsr:106555091"/>
    <dbReference type="CTD" id="1266"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21284">
        <property type="entry name" value="CH_CNN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000040">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF17">
        <property type="entry name" value="CALPONIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="26"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013929338.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="36691" checksum="14076F003231BB80" modified="2020-10-07" version="1">MTHFNKGPSYGLSAEVKNKIALKYDPQVEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLHPGSIKKINQSKLNWHQLENIGNFIKAIQQYGMKPHDIFEANDLFENGNMTQVQTSLVALAGLAKTKGFHTTIDIGVKYAEKQARSFDAGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMPAPGTRRDIYDQKVTLQPIDNTTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQVEYPDDYHGEYQDEYQRDYHGQYSDHGIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0V1</accession>
    <name>A0A6I9Z0V1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Fibrillin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106555211</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929470.1">
        <property type="nucleotide sequence ID" value="XM_014073995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0V1"/>
    <dbReference type="GeneID" id="106555211"/>
    <dbReference type="KEGG" id="tsr:106555211"/>
    <dbReference type="OrthoDB" id="446173at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000023">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000087">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF5">
        <property type="entry name" value="FIBRILLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="11"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929470.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="12661" checksum="2BD9220A2C291033" modified="2020-10-07" version="1">MIKPSLLCLPDENECLSSHVCGGASCHNTLGSYKCMCPTGYNFEQFSGQCQDVNECGSSQAPCSYGCSNTEGGYVCGCPPGYFRIGQGHCVAGMGLGKGQNHELPLSGEIDDSALSPEACY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1S8</accession>
    <name>A0A6I9Z1S8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Retinol-binding protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106556543</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 9">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931003.1">
        <property type="nucleotide sequence ID" value="XM_014075528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1S8"/>
    <dbReference type="GeneID" id="106556543"/>
    <dbReference type="KEGG" id="tsr:106556543"/>
    <dbReference type="OrthoDB" id="9923952at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034632">
        <property type="term" value="F:retinol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000004">
        <property type="entry name" value="Retinol-binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022271">
        <property type="entry name" value="Lipocalin_ApoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002449">
        <property type="entry name" value="Retinol-bd/Purpurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873:SF2">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036893">
        <property type="entry name" value="Lipocalin_ApoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500204">
        <property type="entry name" value="RBP_purpurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01174">
        <property type="entry name" value="RETINOLBNDNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027204137" evidence="7">
        <location>
            <begin position="27"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="10">
        <location>
            <begin position="47"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036893-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013931003.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="24779" checksum="31E87FF8A065F7B4" modified="2020-10-07" version="1">MPFRCPTPAWLMLVGFLALLDSRCEAQRDCRVSSFTVQENFDKYRYAGNWYAIAKKDPEGLFLQDNVVASFTVDEDDKMTATARGRVVLFDTWNMCADMVGTFTETEDPAKFKMKYWGVASYLQKGNDDHWVVATDYDTYALHYSCRNVTEDGTCGDSYSFLFSRTPDGLTPEAQKIVRQKQVEICLDKQYRRIVHDGKEYFIRTLQEIWPRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2R1</accession>
    <name>A0A6I9Z2R1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cartilage matrix protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106555883</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930286.1">
        <property type="nucleotide sequence ID" value="XM_014074811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2R1"/>
    <dbReference type="GeneID" id="106555883"/>
    <dbReference type="KEGG" id="tsr:106555883"/>
    <dbReference type="OrthoDB" id="6022609at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000018">
        <property type="entry name" value="Matrilin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF16">
        <property type="entry name" value="CARTILAGE MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFA" evidence="8">
        <location>
            <begin position="88"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013930286.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="35477" checksum="7DFC377A9999B289" modified="2020-10-07" version="1">MGCENSNSKLIYLALASLHIDFALVVLGYEDKELEVWRKKPASYIKSIMETSVDSDKNEHQELENQGSSADGVRPQRRGAQCRTRPTDLVFIVDSSRSVRPNEFDKVKIFVSQVIEALDVGPNTTRVGVINYASAVKNELSLRTHQTKTSLLQAVRKIEPLSTGTMTGLAIQFAINRAFSEGEGARVRSPEINKVAIVVTDGHPQDGVKDVSARARALGIEIFAIGVGRVDMRTLRQIASQPLDDHVDYVESYSVIAKLSKKFQEAFCVVSDLCATGDHDCQQICVSTGASYACACEVGFTLNNDGKTCSAKENKEHTFRQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2S6</accession>
    <name>A0A6I9Z2S6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Nuclear receptor subfamily 1 group D member 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">NR1D2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931373.1">
        <property type="nucleotide sequence ID" value="XM_014075898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2S6"/>
    <dbReference type="GeneID" id="106556868"/>
    <dbReference type="KEGG" id="tsr:106556868"/>
    <dbReference type="CTD" id="9975"/>
    <dbReference type="OrthoDB" id="7634782at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07166">
        <property type="entry name" value="NR_DBD_REV_ERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06940">
        <property type="entry name" value="NR_LBD_REV_ERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000016">
        <property type="entry name" value="Nuclear receptor subfamily 1 group D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000013">
        <property type="entry name" value="Nuclear receptor subfamily 1 group D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001728">
        <property type="entry name" value="ThyrH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF112">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 1 GROUP D MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00546">
        <property type="entry name" value="THYROIDHORMR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18 20 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="17 20" id="KW-0804">Transcription</keyword>
    <keyword evidence="13 20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="22">
        <location>
            <begin position="98"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="23">
        <location>
            <begin position="367"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013931373.1"/>
        </source>
    </evidence>
    <sequence length="585" mass="64750" checksum="7B2FF5BF8911F5FB" modified="2020-10-07" version="1">MELSVGSVIAYISSSSSASSPASCHSEGSDNSFQSSSSPVPPSPNSSSSEGSCSSKSKEHIDGRPNSEQFENHTKGNQLSGASAAKSHNGFAKFNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKKCLKNNNCSIMRMNRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMIIEMQSAMKTMMNTQFSDHLSSETLKDSQEALLLVPQEQLNSKLKQEEVTAKRPSSSPLPPPLCPALPLPSDIAKEEVIGMVTSAHKDTFMCNQEQPEDRTTVLQPPNGGKNLKYLDQYNSDGEADHGSISSVLQNGNVKHFNGQYKGQNNMHHPSGYNLCFHSMNFTNGFTTKGYNKMLKNGFSSIESLNAYSCSTGGRMHLVCPMNKTPFVDPNKSGQEVWEEFSMSFTPAVKEVVEFAKRVPGFRDLSQHDQVNLLKAGTFEVLMVRFASLFDAKDRTVTFLSGKKYSLDDLRTMGAGDLLNSMFEFSEKLNALNLSDEEMSLFTAVVLVSAERSGIENVNSVEALQETLIRALRTLITKNHPNEASIFTKLLLKLPDLRSLNNMHSEELLAFKVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3I0</accession>
    <name>A0A6I9Z3I0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore complex protein Nup98-Nup96</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NUP98</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931207.1">
        <property type="nucleotide sequence ID" value="XM_014075732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3I0"/>
    <dbReference type="GeneID" id="106556747"/>
    <dbReference type="KEGG" id="tsr:106556747"/>
    <dbReference type="CTD" id="4928"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2360:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21240">
        <property type="entry name" value="Nup98_GLEBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="12">
        <location>
            <begin position="724"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="496"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="543"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931207.1"/>
        </source>
    </evidence>
    <sequence length="770" mass="79303" checksum="2D6B97C724EEB373" modified="2020-10-07" version="1">MFNKSFGTPFGGNTGGFGTTSTFGQNTGFGATSGGAFGTSAFGSNNNAGGLFGSTQAKPGGLFGASTFSQPATSSTSTGFGFGTSTGTSSGLFGTASTGGGLFSTQSNAFAQNKPVGFGNFGTSTSSGGLFGTTNTASNPFGGTSGSLFGATSFSTVPTGTTIKFNAPTGTDTMVKSGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPTNPVVGAGATPGLFGSSTATSSAATGLFGSSTTNTGFSYGANKTAFGTSTTGFGTGTGSLFGSTQQTPSLFSKPFGQPTTAQNTGFSFTNTSTLGQPNTNTMGLFGAAQPTQTGSLFGTAANTNTAAAFGTGTGLFGQPNTGFGVGGSSLFSNKPAGFGTTTTSAPSFGTTTTGLFGFNANATGNSLFGNKPATGALGPGLGAGFGTALNPGQNSLFGSTQPKLGGTLGTGAFGAPGFNTSTATLGFGAQQPAVALTDPNVSAAQQAVLQQHLNSLTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTSGSSKSHLFDGLDDDEPSLTNGAFMPKSLSENHRQDEEQDDDNYAREMSRFLANAIAKPLPLTPENAPHKHHNNSVDDTLVALNVRPALRNGLESSLEEASYHEESLQEELDKESEEQLQCTHPAGEVRALEGLGWLTLGSLRWSIHPLCTNPVNWYHKLALEKARGPPFSSGYGSIYFEGEVNLTNLNLDEIVHVRRKEVIVYPDDELKPPIGEGLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z421</accession>
    <name>A0A6I9Z421_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HDAC9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013931848.1">
        <property type="nucleotide sequence ID" value="XM_014076373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z421"/>
    <dbReference type="GeneID" id="106557200"/>
    <dbReference type="KEGG" id="tsr:106557200"/>
    <dbReference type="CTD" id="9734"/>
    <dbReference type="OrthoDB" id="5232919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF11">
        <property type="entry name" value="HISTONE DEACETYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="12">
        <location>
            <begin position="57"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="259"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="302"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931848.1"/>
        </source>
    </evidence>
    <sequence length="630" mass="70710" checksum="15250F075474AACB" modified="2020-10-07" version="1">MSFTAQDGVSRRDQLLAQQRMHSMINPVDVKSEIPLGLEQVRPLDLRTDVRMMVPMMDPLMQEKQLQHELILIQHQQQIQKQLLIAEFQKQHENLTRQHQAQIQEHIKLQKELLAIKHQQDILEKEQKLEQQRQEQELERHHREQCPPIKSRERSRERAVASTEVKQKLQEFLLSKSAAKDSLSNEKNHSTSRRPKLWHMASHHTSLDQASPPLCGTSSYKYTLPGAQDAKDDFPLRKTGYVESQGDHLEEKVVFGRDSASEPNLKVRSKLKQKVTERRSSPLLRRKDGTMVCSLKKRLTESSVGSSSPGSGPSSPCNCPTRNTENETSVLPLNSSAEVPQHCILINEESMNLLNLYASPSLPNITLGLPAVQPPLTVSSPFAEKQNNEMQSIKQGVAISSQPRGTISSLSSPPPIVPIVPIVPEGRPNYSHQAILQHLLLKEKMRQQKLLPTGTGPFHPPSPLATTESVSPGLRAAHKLPRHRPLNRTQSAPLPQSTLAQLVIQQQHQQFLEKQKQYQQQIHMNKMLSKSIEQLKQPGSLEEAEEELQGEHLMQEEGAASVGVSIRDDSSGISLQNLGKPFGAVKVKEEPKDSDDGIQNQQMESGERSAFMQQVTGTDLAPDCIIKVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5D1</accession>
    <name>A0A6I9Z5D1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="30">Low-density lipoprotein receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">LDLR</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="22">Endomembrane system</location>
            <topology evidence="22">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931166.1">
        <property type="nucleotide sequence ID" value="XM_014075691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5D1"/>
    <dbReference type="GeneID" id="106556718"/>
    <dbReference type="KEGG" id="tsr:106556718"/>
    <dbReference type="CTD" id="3949"/>
    <dbReference type="OrthoDB" id="5958943at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034362">
        <property type="term" value="C:low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000124">
        <property type="entry name" value="Low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000052">
        <property type="entry name" value="low-density lipoprotein receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000002">
        <property type="entry name" value="low-density lipoprotein receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000002">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000005">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000113">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF15">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="19" id="KW-0168">Coated pit</keyword>
    <keyword evidence="16 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0427">LDL</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="30" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="18 30" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="17" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="7 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027028817" evidence="27">
        <location>
            <begin position="25"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="744"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="28">
        <location>
            <begin position="284"/>
            <end position="298"/>
        </location>
    </feature>
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        <location>
            <begin position="385"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="24">
        <location>
            <begin position="432"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="24">
        <location>
            <begin position="475"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="24">
        <location>
            <begin position="519"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="676"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
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    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
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    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
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    <feature type="disulfide bond" evidence="23">
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            <end position="137"/>
        </location>
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    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
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        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
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        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
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    <sequence length="815" mass="90306" checksum="BD02BA43D9747856" modified="2020-10-07" version="1">MSPAGLQTACVALMLLGILSRVSTNERGKCGRNEFQCRNGDCIPYKWICDDSPECRDGSDESLDACKSVTCSSTQFSCGGRINRCIDVSWRCDSEVDCENGSDEDNCPPKRCADHQFQCQNGNCVAFDFVCDEDDDCGDGSDELDCRAPTCNPEMFHCNSSECIPKLWACDSDADCKDGSDESPAFCGIQQGAKKADPCSPLEFHCGSGECIHQRWQCDGSFDCKDRSDEADCGKHRLSPPQSSLQKPHVGFEILRRKRRTNECLADKGGCSHICNDKKIGYECLCPEGFQLIDQRKCEDIDECLNPDACSQVCVNLQGSYKCECKEGYEIDPPTKACKAIGTIAYLFFTNRHEVRKMTLDHSEYTNLIPHLKEAMALDMEISNNKIYWSDNFHKKIFSTPIDKAGNHSHHTTVISSGIKAPDGLAVDWVHHNIYWTDSGLGTISVASSEGLKRKTLIKEVGAKPRAIVVDPVHGFMYWTDWGASAKIAKSGLNGVDSFPLVKEGIQWPNGITLDLITHRLYWVDSKYHSVSSIDVNGENRRTILVDNEMLAHPFGIAVFEDKVFWTDVLNEAIFSANRRTGSNIVKVAENLFSPEDLMLFHSVRQPKGVNWCEKNGVPNGGCQYLCLPAPQINLHSAKYTCVCPDSMHLGTDMRSCTQDPATPVPPPVAATTVHSAKTSPAARLLSTRKTPTQKTDHLPADVLNNVVTNIPEQTTHEMVTLSQQVQNGIAAETDGTERRGPSALAIVLPLALISLVSFGIFLIWKNWRLKNINSINFDNPVYQKTTEDEIHICRSQEGYSYPSRQMVSLEDDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5Z9</accession>
    <name>A0A6I9Z5Z9_9SAUR</name>
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        <submittedName>
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        <name evidence="5" type="primary">AGR3</name>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:dystroglycan binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="1"/>
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        <source>
            <dbReference type="RefSeq" id="XP_013931619.1"/>
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    </evidence>
    <sequence length="164" mass="18914" checksum="A292A7F54A1A8388" modified="2020-10-07" version="1">MHSAVSLSFIVIAVSSNFAIAIKKEKQILQTLSRGWGDDITWVQTYEEGLYQAKQSNKPLMVIHHLEDCQYCQALKKVFAESQEIQEMCQNDFIMLNLMHETTDKNLSPDGQYVPRIIFVDPSLTVRADIIGRYANRLYTYEPQDLSVLIENMKKALRLIQTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z687</accession>
    <name>A0A6I9Z687_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polypyrimidine tract-binding protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106556927</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931446.1">
        <property type="nucleotide sequence ID" value="XM_014075971.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106556927"/>
    <dbReference type="KEGG" id="tsr:106556927"/>
    <dbReference type="OrthoDB" id="296632at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000036">
        <property type="entry name" value="polypyrimidine tract-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000173">
        <property type="entry name" value="polypyrimidine tract-binding protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006536">
        <property type="entry name" value="HnRNP-L/PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01649">
        <property type="entry name" value="hnRNP-L_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="10"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="127"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013931446.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="22714" checksum="5E156CCF068C7BF1" modified="2020-10-07" version="1">MPGVSTGGNTVLLVSNLNEEMVTPQSLFTLFGVYGDVQRVKILYNKKDSALIQMADGNQSQLAMSHLNGQKMYGKIIRVTLSKHQTVQLPREGLDDQGLTKDFGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEDDLRMLFGNTGGTVKAFKFFQRDHKMALLQMSTVEEAIQALIDLHNYNLGENHHLRVSFSKSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z793</accession>
    <name>A0A6I9Z793_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="32">NADPH oxidase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">CGD91-phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Cytochrome b(558) subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Cytochrome b-245 heavy chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Heme-binding membrane glycoprotein gp91phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Neutrophil cytochrome b 91 kDa polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">gp91-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">gp91-phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">p22 phagocyte B-cytochrome</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="37" type="primary">LOC106557320</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="37">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="31">Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. NADPH oxidase complex assembly is impaired through interaction with NRROS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>NADPH + 2 O2 = 2 superoxide + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63180"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013932009.1">
        <property type="nucleotide sequence ID" value="XM_014076534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z793"/>
    <dbReference type="GeneID" id="106557320"/>
    <dbReference type="KEGG" id="tsr:106557320"/>
    <dbReference type="CTD" id="1536"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000030">
        <property type="entry name" value="cytochrome b-245 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000004">
        <property type="entry name" value="NADPH oxidase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF60">
        <property type="entry name" value="CYTOCHROME B-245 HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 34" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="35">
        <location>
            <begin position="287"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00042476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00042502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00042961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00043221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00043223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00049908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00049971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00050020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00050046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="RefSeq" id="XP_013932009.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="65564" checksum="FCA49BCF130086EB" modified="2020-10-07" version="1">MGNWVENEGLSIFVILVWLGLNVFLFCYYYLIYANEWPYYYTRELLGSYLALARAPAACLNFNCLLILLPVCRNLLSFLRGSSACCSVRVRRQLDRNLTFHKLVAWMIALHTAIHTIAHLFNVERLVDARTEKNGSIKCVLSDLGDQSGETYLNFARKKISNPMGGLYVAFTTLAGLSGVIITLCLILIITSSTKTIRRSYFEVFWFTHHLFVIFFVGLVIHGFGRIVRRQTPESLLLHKPEVCAKNFTAWGKMSSCPKPEFAGNPPMTWKWIIGPMVLYICERLVRFWRSQQKVVITKVVTHPFQTFELQMKKKGFKMEVGQYIFVKCPEVSVLEWHPFTLTSAPEEDYFSIHIRVVGDWTEGLFKACGCDKEQLQEAWKLPKIAVDGPFGTASEDVFSYETVMLVGAGIGVTPFASILKSVWYKYCNNAMDLRLKKIYFYWLCRDTHAFEWFADLLQSAEAQMQEKDQTDFLSYNIYLTGWDESQATHFTMHHDEEKDVITGLKQKTQYGRPNWENEFKTIAGQHPNSRVGVFLCGPEALAETLNKMCINNSEADPRGIHFIFNKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7R9</accession>
    <name>A0A6I9Z7R9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein capicua homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CIC</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Transcriptional repressor which plays a role in development of the central nervous system (CNS). In concert with ATXN1 and ATXN1L, involved in brain development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Found in a complex with ATXN1 and ATXN1L.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with ATXN1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931855.1">
        <property type="nucleotide sequence ID" value="XM_014076380.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106557210"/>
    <dbReference type="KEGG" id="tsr:106557210"/>
    <dbReference type="CTD" id="23152"/>
    <dbReference type="OrthoDB" id="10051111at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21990">
        <property type="entry name" value="HMG-box_CIC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000010">
        <property type="entry name" value="Capicua transcriptional repressor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052412">
        <property type="entry name" value="CC-Dev_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032147">
        <property type="entry name" value="Cic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058607">
        <property type="entry name" value="HMG-box_Cic-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058606">
        <property type="entry name" value="HTH_Cic_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13059">
        <property type="entry name" value="HMG-BOX TRANSCRIPTION FACTOR BBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13059:SF13">
        <property type="entry name" value="PROTEIN CAPICUA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16090">
        <property type="entry name" value="DUF4819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF25981">
        <property type="entry name" value="HTH_Cic_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="13">
        <location>
            <begin position="1025"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="11">
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            <begin position="1025"/>
            <end position="1093"/>
        </location>
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            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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    <feature type="region of interest" description="Disordered" evidence="12">
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="546"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1155"/>
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        <location>
            <begin position="1192"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="1312"/>
            <end position="1407"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1990"/>
            <end position="2011"/>
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    </feature>
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        <location>
            <begin position="2026"/>
            <end position="2235"/>
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            <begin position="9"/>
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</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z869</accession>
    <name>A0A6I9Z869_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106557348</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932041.1">
        <property type="nucleotide sequence ID" value="XM_014076566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z869"/>
    <dbReference type="GeneID" id="106557348"/>
    <dbReference type="KEGG" id="tsr:106557348"/>
    <dbReference type="OrthoDB" id="103819at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009401">
        <property type="entry name" value="Med13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051139">
        <property type="entry name" value="Mediator_complx_sub13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06333">
        <property type="entry name" value="Med13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="10">
        <location>
            <begin position="50"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013932041.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="19855" checksum="9549E3CFD64AFA6F" modified="2020-10-07" version="1">MGIFDLLDTGDDLDPDIISILPASPTGSPVHSPGSHFTHGGDMGKGQGTDRLLSTESHDEVPNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQNQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFIMNML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z8E3</accession>
    <name>A0A6I9Z8E3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyltransferase-like protein 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">METTL25</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013932050.1">
        <property type="nucleotide sequence ID" value="XM_014076575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z8E3"/>
    <dbReference type="GeneID" id="106557322"/>
    <dbReference type="KEGG" id="tsr:106557322"/>
    <dbReference type="CTD" id="84190"/>
    <dbReference type="OrthoDB" id="10258156at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052220">
        <property type="entry name" value="METTL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496">
        <property type="entry name" value="CGI-41 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496:SF9">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13679">
        <property type="entry name" value="Methyltransf_32"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="162"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="375"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013932050.1"/>
        </source>
    </evidence>
    <sequence length="621" mass="70357" checksum="BD6FB061E0CC1734" modified="2020-10-07" version="1">MSRCRRDPSPAASRENYPLPPEPSAETVTERLRAVAEFLRWGLPLCQSHTVEFYTRGLWEKFVALPPETVLGALSGDRLQHLLEEPTPQRPLEEVADNSGFYNIFCKNTQRLINVQAFVLAAKYYSMPNLGVCTSLEQILKAMDRKQQPKVEMKTDNFMNDKKSHEVKLMSDLVDGIAKYYGLNQVIDLGSGKGYLSSFLSLQYDLKVYGIDSSNTNTHGANERNRKLKKHWTAYQRHAEANVRDQTLKEAKKSAEQDKEKYNAKTSETWLRTNKSLQSQVQVAVSDALGTESAEISPDPNIGITKNQYELKPEVRVQLIENNISENAFLDILPTDAVEVDCSSRNNCRELCEEEKEQRKAASLKAKASRSKEANIYSPLTSFITAETELRDIVGDLEDCVLVGLHTCGDLAPSTLRLFNVVPEIKAVCSVGCCYHLLSEEHEMHEEGTRGIWGFPLGRYLKEEGWFCGRNARMAACLALERVAVGQMLPTESLFYRAVLQVIVEECYGIKRSDRNIGKVYSKSSSFLDYVRISLKKLELDESKISDSLVLEYLEKYKYRMNELEAFNMLKVVLGPCIEVLILLDRLCFLKEQDNIAWSGLVKLFDAIKSPRCYAVVALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WW82</accession>
    <name>A0A6I9WW82_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">WEE1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013907169.1">
        <property type="nucleotide sequence ID" value="XM_014051694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WW82"/>
    <dbReference type="GeneID" id="106537536"/>
    <dbReference type="KEGG" id="tsr:106537536"/>
    <dbReference type="CTD" id="7465"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14138">
        <property type="entry name" value="PTKc_Wee1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000115">
        <property type="entry name" value="Wee1-like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000217">
        <property type="entry name" value="Wee1-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017164">
        <property type="entry name" value="Wee1-like_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF72">
        <property type="entry name" value="WEE1-LIKE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037281">
        <property type="entry name" value="Wee1-like_protein_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="307"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="28"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="313"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013907169.1"/>
        </source>
    </evidence>
    <sequence length="653" mass="72581" checksum="AABF9B78187404A6" modified="2020-10-07" version="1">MSFLSRQPPSLATGARRLTVRRAAAPIRQKLLFPGGGSDCEEEEEEEEGGYEEEEEEGGGSSGNSTAEDSAFQEPDSPLSVNRTPARLEPQPPRNPEQIGRELASTALPGEEGDSWEEEGFGSSSPVKSPGAYFMAGSPPMKGPRRFYDQSSSPPPRTDTIRYREHPDPSSPLPEYPGTPPHKTFRKLRLFDTPHTPKSLLCRAEGMGSSSAKLRGGSLFMNVGKLVKQECDTGQAPHVNINPFTPDSVFLQSSVGQCRRRKRTHWNDSYDEDMEASDGELEDETVRPAKRISITESNMKSRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRNMRYFGEPELKDLLLQVAHGLKYIHSMSLVHMDIKPSNIFISRTSIPTTISEEGDEDEWSSSRVVFKIGDLGHVTRIASPQVEEGDSRFLANEVLQENYNYLPKADIFALALTLICAAGAEPLPANGDQWHEIRQGRLPKIPQVLSQDLLDLLKVMINPDPEKRPSAGALVKHSALLSTARKSADQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERAQFTDRMTTRSTAQSRIARLTGKKMNRSVSLTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0E0</accession>
    <name>A0A6I9X0E0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Sodium-coupled neutral amino acid transporter 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 38 member 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SLC38A7</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Symporter that selectively cotransports sodium ions and amino acids, such as L-glutamine and L-asparagine from the lysosome into the cytoplasm and may participates in mTORC1 activation. The transport activity requires an acidic lysosomal lumen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-asparagine(in) + Na(+)(in) = L-asparagine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:71383"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamine(in) + Na(+)(in) = L-glutamine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68236"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908704.1">
        <property type="nucleotide sequence ID" value="XM_014053229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0E0"/>
    <dbReference type="GeneID" id="106538662"/>
    <dbReference type="KEGG" id="tsr:106538662"/>
    <dbReference type="CTD" id="55238"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015182">
        <property type="term" value="F:L-asparagine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000038">
        <property type="entry name" value="Putative sodium-coupled neutral amino acid transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF192">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="20">
        <location>
            <begin position="40"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013908704.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="49702" checksum="4AC0C7AC1DB83405" modified="2020-10-07" version="1">MTQSHVGINRDYGDWEWSADAGERARLLQSPSVEERRPATTSMLGAVFIVVNAALGAGLLNFPAAFQMAGGVGAGIAMQMGLLLFIIGGLVILAYCSQASNERTYQEVVWAVCGRVLGVLCEVAIVVYTFGTCIAFLIIIGDQQDKIIPVLIKSCAREGKSCWFTDRKFTISLTAILFILPLSIPKEIGFQKYASCLSVIGTWYVTIVVIVKYFWPDQGISPEAITTSPSSWVSVFNAMPTICFGFQCHVSSVPIFNSMKCPQVRSWGVVVTAAMVIALFVYMGTGVCGFLTFGENVQQDVLLSYPSDDIPVAFARAFIILSVLTSYPILHFCGRTVLEGLWLRYKGESMEEDVTRERRRRLLQTFCWFLLTLLLALFIPDIGRVISVIGGLAACFIFVFPGLCLIHTKLSEVQGAGGVSWWMVVGYGMFMVVLGAFIFGQTTANAISMDLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1I7</accession>
    <name>A0A6I9X1I7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Deleted in autism-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106538975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909114.1">
        <property type="nucleotide sequence ID" value="XM_014053639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1I7"/>
    <dbReference type="GeneID" id="106538975"/>
    <dbReference type="KEGG" id="tsr:106538975"/>
    <dbReference type="CTD" id="79742"/>
    <dbReference type="OrthoDB" id="10035316at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020519">
        <property type="entry name" value="DIPK2A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073:SF8">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073">
        <property type="entry name" value="GH11358P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027041001" evidence="5">
        <location>
            <begin position="23"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="FAM69 protein-kinase" evidence="6">
        <location>
            <begin position="205"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909114.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="49041" checksum="31E16C1BD0AC9A09" modified="2020-10-07" version="1">MLEMYHLVSKFLLSLLSFTVNCNLFLESTVSPSSGPRVKSSYTFGRTFLGLDKCNACIGTSICKKFFKEEIRFDSWLSSHLILPPSSLSRYPGNYSDDSKSWRLVEISRLTTKYQHDESDERICTFLTQTKRCSIERALWKTERFQKWLKAKRLTPLLVQGLRTPMLRCPSQRLLDRIVRRYAEVPDAGSVYMDHFTDRDKLRLLYMLSINTHPIILQIFPGAEGWPFPKYLGSCGRLIVTASTRPLKEFYGSSSDVTADLALQLLTIIDFLMNNDLNYFFYFTHVDADTFGVFNNGQLFIQDASMLGVIDKQEGRDLMNRQQKYKDIFSCLAVDCEPVLPSCSSIKGSQNLIMICKKLLPNLLKQKFQSPLQEKINSALSICADSFLSDQEIITATQLLVDILKPLQICDSRFVYRYPDCKYSTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X387</accession>
    <name>A0A6I9X387_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Paired box protein Pax-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106539479</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909754.1">
        <property type="nucleotide sequence ID" value="XM_014054279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X387"/>
    <dbReference type="GeneID" id="106539479"/>
    <dbReference type="KEGG" id="tsr:106539479"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00131">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000710">
        <property type="entry name" value="Paired box 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000080">
        <property type="entry name" value="paired box protein Pax-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000031">
        <property type="entry name" value="Paired box protein Pax-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF26">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0563">Paired box</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="14">
        <location>
            <begin position="34"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="214"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="166"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013909754.1"/>
        </source>
    </evidence>
    <sequence length="269" mass="30568" checksum="ECACCF223BFF0363" modified="2020-10-07" version="1">MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPRVATPDVEKKIEEYKRENPGMFSWEIRDRLLKDGHCDRTTVPSGLVSSISRVLRIKFGKKEEEEDHEKKEEDCEKKAKHSIDGILGDKGGRLDDGSDVESEPDLPLKRKQRRSRTSFTAEQLEELEKAFERTHYPDIYTREELAQRTKLTEARVQVWFSNRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X457</accession>
    <name>A0A6I9X457_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12 13">Growth factor receptor-bound protein 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">GRB10</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GRB7/10/14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909086.1">
        <property type="nucleotide sequence ID" value="XM_014053611.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909087.1">
        <property type="nucleotide sequence ID" value="XM_014053612.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909088.1">
        <property type="nucleotide sequence ID" value="XM_014053613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X457"/>
    <dbReference type="GeneID" id="106538956"/>
    <dbReference type="KEGG" id="tsr:106538956"/>
    <dbReference type="CTD" id="2887"/>
    <dbReference type="OrthoDB" id="6278443at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005158">
        <property type="term" value="F:insulin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01259">
        <property type="entry name" value="PH_APBB1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10415">
        <property type="entry name" value="SH2_Grb10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000062">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000056">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000015">
        <property type="entry name" value="Growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015042">
        <property type="entry name" value="BPS-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035037">
        <property type="entry name" value="Grb10_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039665">
        <property type="entry name" value="PH_APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF4">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08947">
        <property type="entry name" value="BPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 12 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Ras-associating" evidence="9">
        <location>
            <begin position="163"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="289"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="492"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013909086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013909088.1"/>
        </source>
    </evidence>
    <sequence length="604" mass="68758" checksum="2208806B12DA79C4" modified="2020-10-07" version="1">MALAGCPDSFLHHPYRIDPVDQTSAHTGRDLVEPGYQKQSSHISLNHQGDDDVDLEALVNNMNSSFENLCSTFCVHSETTPLLQNGSQVLASGTSSLHPVSPRQKIQRSQPVRIMAVRRLQEEEQQFRTSSLPAIPNPFPELCSPTNSPVLAPGSLPQSQPMNKQDIKVFSEDGTCKVVEILADTTARDLCQLLVYKSHCVDDNSWALVEHHPLIGLERCLEDHELLAQVESTMGNGSKFLFRKNYAKYEFFKNPVNFFPEQMIAWCQQSNGCIPQSQLLQKFLNSNSCPEIQGFLHVKELGRKSWKKLYGCLRKSGLYCSTKGSSKEPRHLQLIADLEDSNIFSLIACKKMYSAPTDYGFCIKPNRVRNEAKELRLLCAEDEQSRTCWMTAFRLLKYGMLLYQNYRIPQQRNNLLPHFATPARSVSENSLVAMDFSGQIGRVIENPEEAQCAALEEGHAWRKRSTRMNILGNQSPLHPSLLSTIIHRTQHWFHGRISREESHRIIKQQGLVDGLFLLRDSQSNPKAFVLTLCHHQKIKHFQILPHFIFVSFSFFKCEDDGQIFFSLDDGSTKFTDLIQLVEFYQLNKGVLPCKLKHHCIRVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4H0</accession>
    <name>A0A6I9X4H0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">lysoplasmalogenase</fullName>
            <ecNumber evidence="6">3.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106538703</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = a 2,3-saturated aldehyde + sn-glycerol 3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:22544"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = a 2,3-saturated aldehyde + sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:16905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
            <dbReference type="ChEBI" id="CHEBI:143890"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM86 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013908752.1">
        <property type="nucleotide sequence ID" value="XM_014053277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4H0"/>
    <dbReference type="GeneID" id="106538703"/>
    <dbReference type="KEGG" id="tsr:106538703"/>
    <dbReference type="OrthoDB" id="2133758at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047408">
        <property type="term" value="F:alkenylglycerophosphocholine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012506">
        <property type="entry name" value="TMEM86B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885">
        <property type="entry name" value="GH04784P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885:SF12">
        <property type="entry name" value="LYSOPLASMALOGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07947">
        <property type="entry name" value="YhhN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013908752.1"/>
        </source>
    </evidence>
    <sequence length="105" mass="11841" checksum="9CEA10AECBF2E255" modified="2020-10-07" version="1">MDILELDAQYKRRVAGNKRTMLLRLLPFVVSCIIYFVLLLPEPSLKSTVIKCLPTFSLAFFVATQSKNGGNLKLYSRWIFIGLLFACVGDACLVWPEFFLADSAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X588</accession>
    <name>A0A6I9X588_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Testican-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106539205</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">May participate in diverse steps of neurogenesis. Inhibits the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. May interfere with tumor invasion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909421.1">
        <property type="nucleotide sequence ID" value="XM_014053946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X588"/>
    <dbReference type="GeneID" id="106539205"/>
    <dbReference type="KEGG" id="tsr:106539205"/>
    <dbReference type="OrthoDB" id="8875634at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16237">
        <property type="entry name" value="EFh_SPARC_TICN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000053">
        <property type="entry name" value="Putative testican-3 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000003">
        <property type="entry name" value="SPARC/osteonectin, cwcv and kazal-like domains proteoglycan 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000001">
        <property type="entry name" value="Testican-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866">
        <property type="entry name" value="SPARC OSTEONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866:SF17">
        <property type="entry name" value="TESTICAN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="12" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="4" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="19">
        <location>
            <begin position="158"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="18">
        <location>
            <begin position="335"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="434"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="373"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013909421.1"/>
        </source>
    </evidence>
    <sequence length="461" mass="51689" checksum="027BCFF021BACAD2" modified="2020-10-07" version="1">MRCSKSTDNLPVQLHFAQANLLSKTVQLAIEFSDSTLPIMAAWVYLALITYLSPTGLSCQYDIRKDTKALILIVLQGAINSYETVQVMDDYFRNWNPNKPFDQALDPSKDPCLKVKCSPHKVCVTHDYETATCVSRKQLVHSLRQKKGNLPQRHWAGSSNLVKCKPCPLTHASSVCGSDGHTYTTKCKLEFHACTSGKNIVARCEGPCPCLPGQESPKHKTACTDKELRNLASRLKDWFGSLHEDANRVIKPTSSDSAQGRFDTSILPICKDSLGWMFNKLDMNYDLLLDHSEINAIYLDKYEPCIKPLFNSCDSFKDGKLSNNEWCYCFQKPGGLPCQNEMNRIQKMGKGKNLMGAFIPRCNEEGYYKATQCHGSTGQCWCVDKYGNEIAGSRKQGTVSCEEEQETSGDFGSGGSVLLLDDLEEAYEAAKKDKQKIHVRATNEDDEDEDDDKDDEIGYIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6C7</accession>
    <name>A0A6I9X6C7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription cofactor vestigial-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VGLL3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a specific coactivator for the mammalian TEFs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the vestigial family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911009.1">
        <property type="nucleotide sequence ID" value="XM_014055534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6C7"/>
    <dbReference type="GeneID" id="106540436"/>
    <dbReference type="KEGG" id="tsr:106540436"/>
    <dbReference type="CTD" id="389136"/>
    <dbReference type="OrthoDB" id="10069705at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006627">
        <property type="entry name" value="TDU_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011520">
        <property type="entry name" value="Vg_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15950">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15950:SF16">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07545">
        <property type="entry name" value="Vg_Tdu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00711">
        <property type="entry name" value="TDU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013911009.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="34720" checksum="5475D1648C862834" modified="2020-10-07" version="1">MSCSDVVRHQAQATRRPSQVKLPAASAVCSTSPCPFSTPAFTPSQQNKIVVYSKMQETLEVTLSNKQEEEEKDQPTEMEYLNSRCVLFTYFQGDIGSVVDEHFSRALTQANNFNSEAVLSKTKTDLWRENTTIANQRRNFPASFWTSSYQTPPPPSLSGIHRDFPITIPSTFSVADPNSWPGHTLHQTAPPPSSNMPESWHYPLASQVSSSYGHMHDVYMHHHHPHVHMHHHHHPTSHLDPRYRPLPVPSVCGGKIPPLQCDATKSDPTTVSCATSAWAGAFHGTVDIVPTFGFETAGTQHQDKTKEALWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAL9</accession>
    <name>A0A6I9XAL9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Transcription factor EB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">TFEB</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MiT/TFE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911356.1">
        <property type="nucleotide sequence ID" value="XM_014055881.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911357.1">
        <property type="nucleotide sequence ID" value="XM_014055882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAL9"/>
    <dbReference type="GeneID" id="106540680"/>
    <dbReference type="KEGG" id="tsr:106540680"/>
    <dbReference type="CTD" id="7942"/>
    <dbReference type="OrthoDB" id="6242697at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006959">
        <property type="term" value="P:humoral immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18927">
        <property type="entry name" value="bHLHzip_TFEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000003">
        <property type="entry name" value="microphthalmia-associated transcription factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024098">
        <property type="entry name" value="bHLHzip_TFEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021802">
        <property type="entry name" value="MiT/TFE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031867">
        <property type="entry name" value="MiT/TFE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR EB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11851">
        <property type="entry name" value="DUF3371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15951">
        <property type="entry name" value="MITF_TFEB_C_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="228"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="100"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="360"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="435"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013911356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013911357.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="52259" checksum="A4D46C1D2AB911B0" modified="2020-10-07" version="1">MASRIGLRMQLMREQVQQEEQRERMQQQTMMHYMQQQQQMPMAPTPAINTPVHFQSPPSVPGEVLKVQSFLENPTTYHIQRSRDKKVQEYLSETYGNKFAPLLGAPSPKPPPSASPGVRPCHAMPSSAGNSTPNSPMAMLNISSNPEREIDDVIEDIMQLTTTVGCLNPEIHMPNTLPLSSSHMNVYNSDLQMTPSVVGITSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKASDLDVRWNKGTILKASVDYIRRMQKDLQRSRDLENHSRRLEMANKQLWLRIQELEMQARVHGLPTASPSGTNMAELAQHMVKQETSGEEGTAELLQQPQLQAEQEAQHLPHFAPPPPPPPQSPYHQLDFTHSLSFDDSCRGFHDPLDPSQNVSFPSLSKKELDLMLMEDTMLPVASDPLFSTVSPEASKASSRRSSFSMEDTDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBL8</accession>
    <name>A0A6I9XBL8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 21 22 23" type="primary">DHX58</name>
    </gene>
    <organism evidence="19 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RLR subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013908575.1">
        <property type="nucleotide sequence ID" value="XM_014053100.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908577.1">
        <property type="nucleotide sequence ID" value="XM_014053102.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908578.1">
        <property type="nucleotide sequence ID" value="XM_014053103.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908579.1">
        <property type="nucleotide sequence ID" value="XM_014053104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBL8"/>
    <dbReference type="GeneID" id="106538582"/>
    <dbReference type="KEGG" id="tsr:106538582"/>
    <dbReference type="CTD" id="79132"/>
    <dbReference type="OrthoDB" id="416741at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140374">
        <property type="term" value="P:antiviral innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002753">
        <property type="term" value="P:cytoplasmic pattern recognition receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039536">
        <property type="term" value="P:negative regulation of RIG-I signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12090">
        <property type="entry name" value="MDA5_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.30">
        <property type="entry name" value="RIG-I-like receptor, C-terminal regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041204">
        <property type="entry name" value="RIG-I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038557">
        <property type="entry name" value="RLR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021673">
        <property type="entry name" value="RLR_CTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051363">
        <property type="entry name" value="RLR_Helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14074:SF7">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14074">
        <property type="entry name" value="HELICASE WITH DEATH DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18119">
        <property type="entry name" value="RIG-I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11648">
        <property type="entry name" value="RIG-I_C-RD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51789">
        <property type="entry name" value="RLR_CTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 20 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="11"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="358"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="RLR CTR" evidence="18">
        <location>
            <begin position="543"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013908575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013908577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013908578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013908579.1"/>
        </source>
    </evidence>
    <sequence length="682" mass="78236" checksum="E1F6A4149031F6A3" modified="2020-10-07" version="1">MELRDYQWEVIGPALEGKNIIICLPTGAGKTRAAVYVCKKHLESHDKNKVVVLVNTVPLLDQHLKNEFSVLQNQFQITSVCGDSFQKLFFSDVVKTYDLIICTAQIFYNALTNQEEEMHVELTDFSLLVIDECHHTHKGTVYNKIMENYLDYKLKGQQGLPQILGLTASLGTGSSNTVEAAKAHILEICANLDAERIMSIDKQNPSLDDHVPCPKKQYDLTEPRLQDPFGEKLREIMTQIYTYLNVSDITANFGTKGFEQQIVELEMKGAKECCQKTRVCALHLRKYNDALLTNETVRMIDAFNILDEFYLVERATKKLIKKSEQYLFQLFDENRLTLLDLAKDSRYENPKLGKLQQVLQDQFQELKSSRGIVFIWTRQSAYSLQEWVLGNQALRALGIKSDVLTGAGSSSQTKHMTQQEQQDVLDRFRKGQLNLLLSTSVAEEGLDIPECNIVIRYGLMTNEIAMMQARGRARAPNSICSILAKTNSKEVARERLNESLEILMERAIKWVQEMPEEEYHQKIAKLQHESMISRKLRDVKLQQQRHLHNPDSVIFCCLNCNLAVCHASDLRKIENMHHININSNFSLYYTKSPNCVTLSREFKDWKPGSSISCETCGQIWGMEMIYRDFTLPMLAIKNFVVETPSGRQKPKKWSKVTFEIKDFDYSEYCASLELDTQDLGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBP9</accession>
    <name>A0A6I9XBP9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA repair-scaffolding protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106540925</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013911706.1">
        <property type="nucleotide sequence ID" value="XM_014056231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBP9"/>
    <dbReference type="GeneID" id="106540925"/>
    <dbReference type="KEGG" id="tsr:106540925"/>
    <dbReference type="OrthoDB" id="1914453at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070202">
        <property type="term" value="P:regulation of establishment of protein localization to chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053054">
        <property type="entry name" value="DNA_repair-scaffolding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028026">
        <property type="entry name" value="DUF4502"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028032">
        <property type="entry name" value="DUF4503"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34347:SF1">
        <property type="entry name" value="DNA REPAIR-SCAFFOLDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34347">
        <property type="entry name" value="DNA REPAIR-SCAFFOLDING PROTEIN SPIDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14950">
        <property type="entry name" value="DUF4502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14951">
        <property type="entry name" value="DUF4503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4502" evidence="2">
        <location>
            <begin position="5"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4503" evidence="3">
        <location>
            <begin position="521"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013911706.1"/>
        </source>
    </evidence>
    <sequence length="818" mass="92555" checksum="0B4BD0E4A3115855" modified="2020-10-07" version="1">MPRQEEKKNLHAPVAAASISDTWHHCGDGFQISSSLEAKESLRKPVRAKKSFASLLSSSEEISQTENLCEFTDIVWSEFSDDENDITASDFLYMKMKKYRKYALIAKEVCNEEPEFIEWKNDSDYEHDEQCSESEGNEAPLDILDTDSCTNSTSKANGEKEDEPSKISEYSSDDDNFEGSNVETEATNLAFPKKFQFCFGVGFETDTGRSATDWLKSAQTFLQTPKKKIEKSLKTPEDSAKKRKLLRRGLAERLNRLQNRKRSAVNFWRYQYTSHHDIVSGGKPDVLIVKILEMHEECTLCIAICHQLGRICDDVSIIKEIVDSQPKLKVLFAKETTAHLKAVPQDVIYIHPPWQKLFLQNENIPVILNTYFSQKVTLKESTDISTEMDKPLLQEPMLTKRNVSLAWIFNLSDIKDNCSLTTAVNQISCSNMKTSKNTESCFNIESKVSVISLNDSLLDAVEMQGTTESTAIQVQVVIQRIYYLVVEDGFGCHPHGSNLSQNMAPFLKSDLQNLRLCLLAQDICGIFSEIQLPILCFSSQVIEQYRKKWEGKCCCVSRLKILRRLTRGRTLGLFSLIDSLWPPLSPLQVPGKRQGNKAHVTANQLPQSFCYILAASADYIDADEREQLSNLYFPPIVHSLEQIYQMGDLPQRCSFWAHVIYRRLQEMGSTSPDQRQFWLFVTDFSLQRESEINSGTPKTLPVSIASSCVIDEEVMEAFKNSSPCIIFFKDAMCGNGKIICIERTVLLLQKPLLCRAAGADITELTGPVKLDYLDSTTQINSICTVRGTVVGVNEKTAFSWPICNRCGNEKLDQHPQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBW8</accession>
    <name>A0A6I9XBW8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Regulator of G-protein signaling 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RGS1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913074.1">
        <property type="nucleotide sequence ID" value="XM_014057599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBW8"/>
    <dbReference type="GeneID" id="106542002"/>
    <dbReference type="KEGG" id="tsr:106542002"/>
    <dbReference type="CTD" id="5996"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF34">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="8">
        <location>
            <begin position="105"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013913074.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="26623" checksum="43A3FF3D797ADCD1" modified="2020-10-07" version="1">MSQCIKPSALQEVRSLDVTTAEALQPKTRQLKMPWLFMWHTASDEIKTHENAGSKNHRKKTKALGVEAKKYFMGLVPPAESADMKYSSSRDVMLSAKEVIQWSQSLEKLLATQRGQEAFREFLKSEFSDENIEFWLACEDYRKTQADCLHGKAEKIYQEFVKSDARKQINIDFPIRKSVAKKVQDPTPSSFDEAQRLIYVLMERDSYPRFLKSQHYHNLLNKVQSNRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD85</accession>
    <name>A0A6I9XD85_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Voltage-dependent calcium channel subunit alpha-2/delta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CACNA2D2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel subunit alpha-2/delta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913579.1">
        <property type="nucleotide sequence ID" value="XM_014058104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD85"/>
    <dbReference type="GeneID" id="106542392"/>
    <dbReference type="KEGG" id="tsr:106542392"/>
    <dbReference type="CTD" id="9254"/>
    <dbReference type="OrthoDB" id="10054666at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000006">
        <property type="entry name" value="voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026927610" evidence="17">
        <location>
            <begin position="21"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="184"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013913579.1"/>
        </source>
    </evidence>
    <sequence length="1063" mass="121094" checksum="CBB9F7FE74998A80" modified="2020-10-07" version="1">MHLQHLSLVIIAIHTVSLWTHTISSLSFQRLADAAEKFQKAHRWQDNIKEEDIEYYDSKADTEYDDPEGEEIEKEKFNSLKLEFTDDDNFKTKVNYSYAAVQIPTDIYKGSTVILNELNWTEALEDVFVENRKEDPSLLWQVFGSATGVTRFYPASPWRAPNKIDLYDVRRRPWYIQGASSPKDMVIIVDVSGSVSGLTLKLMKTSVYDMLDTLSDDDYVNVASFNQKAQPVSCFKHLVQANIRNKKVFKEDVEGMEAQGTTDYKAGFEYAFEQLQNSNITRANCNKMIMMFTDGGEDRVQDVFEKYNGPNKTVRVFTFSVGQHNYDVTPLQWMACANKGYYFEIPSIGAIRINTQEYLNVLGRPMVLAGNKAKQVQWTNVYQDALGLGLVVTGTLPVFNLTDDGQSRGSSEWKNQLILGVVGIDVALNDIKKLTPRYNLGANGYVFAIDLNGYVLLHPNLRPLIINFQEAVTLDFLDAELEDENKERIRRSMIDGNKDQQYIKTLIKSLDERYVDKVHRTYTWAPIKSTNYSLGLVLPNYSRSYIQANLSDQILQVKLPSKLKDFEYLLPNSFESEGHVFIAPREYCKDLNLSDNNTEFLENFIALMEKVTPDSKQCDNFLLHNLILDTGITQQLVETVWKNQDLSTYSLLAVFVATDGGITRVFPNKAAEDWEEDREPFNASFYRRSLDNKGYIFKPPYRDPSYRGSEPESNPVGILVSTAVELNLAERHLKPAVVGVKLDLEAWTQKFKVLASNQTDQQKTRRCDLSTSCEMDCDTNDKDLLCVLIDDGGFLVISNQEDYKYQVGKFFSEVDAHLMSALYNNSFFTQKESYDFQSVCAPEAPSNTGSAPRGVYVPTMADFLNLAWWSSAAAWSLFQQFLYGLAYNSWFQTEDVSADNMESKETSCIMKQTQYYFSSVNYTYNTIIDCGNCSRLVQRAPLCDLSTSCEMDCDTNDKLPFTLIQLQILERGKQKQESKEEQKPITNVGLSAPPGDRNQCELVEKPRYRKGPRVCFDYNITEDTSDCGRGASFRPSLKILLCLQLLLLYLTASHHSLPLAFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEV5</accession>
    <name>A0A6I9XEV5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Equilibrative nucleoside transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLC29A1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909603.1">
        <property type="nucleotide sequence ID" value="XM_014054128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEV5"/>
    <dbReference type="GeneID" id="106539343"/>
    <dbReference type="KEGG" id="tsr:106539343"/>
    <dbReference type="CTD" id="2030"/>
    <dbReference type="OrthoDB" id="46396at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015213">
        <property type="term" value="F:uridine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034764">
        <property type="entry name" value="ENT1/ENT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00939">
        <property type="entry name" value="2a57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF9">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013909603.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="36728" checksum="F609B92F456CDB41" modified="2020-10-07" version="1">MVTAIVVKVPMEPLTFFIVTMVKIVLINSFGAILQGSLFGLAGLLPASYTAPIMSGQGLAGTFAAVAMICAIATGSELKESAFGYFITACVVIMIAIGSYIMLPRLDFFCFYSMKNKREYATSATQAEMETKVVFIKKDVVNGVERNHSGNGESLASSHRSSVLDIFKKIWVMAASVCLVFTVTIGVFPAVTVEVKPTIGKDTPWVLYFSPVACFLMFNIFDWAGRSLTAVCVWPGKESRLLPFLVIIRLIFIPLFMLCNVEPRHNLPVIFAHDAWFLAFMLLFSISNGYLASLCMCFGPKKVLSHEAETAGAIMAFFLSLGLALGAILSFPLRAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEZ4</accession>
    <name>A0A6I9XEZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein DLX-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DLX1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909638.1">
        <property type="nucleotide sequence ID" value="XM_014054163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEZ4"/>
    <dbReference type="GeneID" id="106539377"/>
    <dbReference type="KEGG" id="tsr:106539377"/>
    <dbReference type="CTD" id="1745"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000074">
        <property type="entry name" value="Distal-less homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF33">
        <property type="entry name" value="HOMEOBOX PROTEIN DLX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="27"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909638.1"/>
        </source>
    </evidence>
    <sequence length="156" mass="17173" checksum="148CBA42B29CC418" modified="2020-10-07" version="1">MYAIKGTETEKSTVVEGGEVRFNGKGKKIRKPRTIYSSLQLQALNRRFQQTQYLALPERAELAASLGLTQTQVKIWFQNKRSKFKKLMKQGGAALESSALANGRSLSANSPPGPPVWNTSSSSGKSSTGTPGTYIPSYTSWYPSAHQETMQQPQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEZ8</accession>
    <name>A0A6I9XEZ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Germ cell-specific gene 1-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106541751</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912756.1">
        <property type="nucleotide sequence ID" value="XM_014057281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEZ8"/>
    <dbReference type="GeneID" id="106541751"/>
    <dbReference type="KEGG" id="tsr:106541751"/>
    <dbReference type="OrthoDB" id="10001768at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012478">
        <property type="entry name" value="GSG-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF40">
        <property type="entry name" value="GERM CELL-SPECIFIC GENE 1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07803">
        <property type="entry name" value="GSG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912756.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="31859" checksum="B06BA20A5AA00AA9" modified="2020-10-07" version="1">MGVDRRQRASAALSLGFLSLIFSITAFSSSYWCEGTQKVAKPFCNGLAGGTHCIGMNSPGGNDSSVVQYIWETGDNKFVDRKFHAGIWHSCEEMISGKGVLWLSIAAEILYIILLLMGITLMLIEICRSISVLDGLKLNAFAAIFTVLAGLLGMVAHMMYTTVFQMTVNFGPEDWRPQMWDYGWSYCLAWVSFACCMASSVTTMNRYTKTILEFKYKQKKLERSLQAKSNPPVPEKAWKMYVDTLQRTTEDFMHPLTDIHNPSSPMEFAELNNIPLAHCEEYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFE0</accession>
    <name>A0A6I9XFE0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Monocarboxylate transporter 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SLC16A14</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912891.1">
        <property type="nucleotide sequence ID" value="XM_014057416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFE0"/>
    <dbReference type="GeneID" id="106541863"/>
    <dbReference type="KEGG" id="tsr:106541863"/>
    <dbReference type="CTD" id="151473"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17429">
        <property type="entry name" value="MFS_MCT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF239">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="37"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013912891.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="56827" checksum="8E9DF6D493F71609" modified="2020-10-07" version="1">MYDSHEDVDSDSEKNSKEREQKLNSNPDIDRGWAWMIVFSSFLVHILIMGSQMALGILNMEWLEEFSQSRGLTAWVSSLNMGITLIVGPFIGLFINTCGCRKTAITGGILNALGWILSSYASNVHYLFITFGIIAGIGSGMVYLPAVVMVGEYFQKRRALAQGLSTTGTGFGAFLMTALLKYLCLEFGWRNAVFLHGAVCLNLCVCGALLRPICYKKEATEQSKDGSKSQSNSPTKVISCSAEVLTSNCVLREAKKEAKGGQGTKENIIALENKDGIRDQKEICTLGILKRVGQLTVTSQKGFRTWYSSYFGTASLFTNRTFAAFVFWALFAYSTFVIPFIHLPEIVKQYRLSAQNDIFPLTSIIAIVHILGKVVLGIISDLPCISVWNVFMAANFTLVSCIFILPLMHTFASLAVVCALIGFSSGYFSLMPVVTEDLVGIKHLANAYGIIICANGISALLGPPFAGWIYDLTQKYDFSFYICGFLYMVGILTLLIQPYIDKEKPASEKHIENGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGY4</accession>
    <name>A0A6I9XGY4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Meckelin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106542235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913376.1">
        <property type="nucleotide sequence ID" value="XM_014057901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGY4"/>
    <dbReference type="GeneID" id="106542235"/>
    <dbReference type="KEGG" id="tsr:106542235"/>
    <dbReference type="OrthoDB" id="419138at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019170">
        <property type="entry name" value="Meckelin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274">
        <property type="entry name" value="MECKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09773">
        <property type="entry name" value="Meckelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013913376.1"/>
        </source>
    </evidence>
    <sequence length="268" mass="29666" checksum="235B8E2E21686C5D" modified="2020-10-07" version="1">MSLWILDDPLSLQEAPWAFVGPLFPAGAPALFCKAEWICLFRNPDLPKLWYGGPTRTFPSVAFGKHSKMDFKVIQYDARGKFLEWQDVKGATLQLCPNSQKILDAAFTMGTTYRQSCALEVSALLQRTPEPIFYEMFLQFEDEKGNTQLWPVPISNPAIVTNNRAPPLNQALRRFFLVDGLSDRKGNLSNAPASVTLAKELLLSVHLPTTAPGEDPPFSLTVRYATHRTPGIAQVSFSVSYNQSPGSAQLATDVSKPSARFSDCLLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHC8</accession>
    <name>A0A6I9XHC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Angiotensinogen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Serpin A8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">AGT</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Stimulates aldosterone release.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915119.1">
        <property type="nucleotide sequence ID" value="XM_014059644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHC8"/>
    <dbReference type="GeneID" id="106543591"/>
    <dbReference type="KEGG" id="tsr:106543591"/>
    <dbReference type="CTD" id="183"/>
    <dbReference type="OrthoDB" id="7817921at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003081">
        <property type="term" value="P:regulation of systemic arterial blood pressure by renin-angiotensin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000227">
        <property type="entry name" value="Angiotensinogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF13">
        <property type="entry name" value="ANGIOTENSINOGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00654">
        <property type="entry name" value="ANGIOTENSNGN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0838">Vasoactive</keyword>
    <keyword evidence="10" id="KW-0839">Vasoconstrictor</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026931626" description="Angiotensinogen" evidence="16">
        <location>
            <begin position="20"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="17">
        <location>
            <begin position="95"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013915119.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="51804" checksum="C7FAD3B6B4A8E000" modified="2020-10-07" version="1">MKLGVILLCVLLYLTTGGCDRVYVHPFFLVDFNNSNANSCKELGEREPLVKTFVPLSIESHFTSTYEESLRNKSEEEEKRLGVNFLKDPISILGARFYKELKKIHENSNIILSPIFIYESLLSIYLGASGETATNLQILLGFNPPSSDSHCTFKIDGHKVLFALKIILNAPLRFRDTEGLIFSKLLCLFSAPGAHLSESFVHELAFPDVNFHIRAVDFTNPTEAAKQINAFVESKSAHRIKGLLADIDQETTLLLTTYTHFKAVINGASPLKEPQEFWLDSQTKIFVPMMTVTGVFKHKYHAGLSIVKIPLSKSLFLLLLQPSNSNQLHSDDQYSLTPSLEWFQGLKSKQIHLTLPRVSFNSIFDLQELLGKQGMPNLLGKEANLRLLSNSNVTIGKIINQQLFELSPSEEDPVEAHTEENASEKLKITFNRAFWFRMYEKESAALIYFGQITNPLNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIQ7</accession>
    <name>A0A6I9XIQ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCN family member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cellular communication network factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein CYR61</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CYR61</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913807.1">
        <property type="nucleotide sequence ID" value="XM_014058332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIQ7"/>
    <dbReference type="GeneID" id="106542553"/>
    <dbReference type="KEGG" id="tsr:106542553"/>
    <dbReference type="CTD" id="3491"/>
    <dbReference type="OrthoDB" id="365605at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010556">
        <property type="term" value="P:regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000015">
        <property type="entry name" value="CYR61 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000038">
        <property type="entry name" value="CYR61 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012395">
        <property type="entry name" value="IGFBP_CNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF18">
        <property type="entry name" value="CCN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036495">
        <property type="entry name" value="IGFBP_rP_CNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
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        <property type="entry name" value="VWFC_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027099211" description="CCN family member 1" evidence="12">
        <location>
            <begin position="25"/>
            <end position="375"/>
        </location>
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    <feature type="domain" description="IGFBP N-terminal" evidence="15">
        <location>
            <begin position="23"/>
            <end position="95"/>
        </location>
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    <feature type="domain" description="VWFC" evidence="14">
        <location>
            <begin position="99"/>
            <end position="165"/>
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            <begin position="281"/>
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            <dbReference type="ARBA" id="ARBA00004613"/>
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            <dbReference type="ARBA" id="ARBA00008125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00042351"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
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            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="PROSITE" id="PS01225"/>
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        <source>
            <dbReference type="PROSITE" id="PS50184"/>
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        <source>
            <dbReference type="PROSITE" id="PS51323"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013913807.1"/>
        </source>
    </evidence>
    <sequence length="375" mass="41596" checksum="920957E93309CD65" modified="2020-10-07" version="1">MNSQRTSCWILAFAVLQLVNRVISSSCPDTCECPLRVPKCAPGVGLVLDGCKCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSKIYQNGESFQPNCKHQCTCIDGAVGCIPLCLQKLSLPFLGCPNPRLVKVPGQCCEEWVCDDSKDNLEQVEGFFGKGFDVDASEGELTSNNELATIKRKRRILPVFETDIHSHSFENPKCLVQTTSWGPCSKTCGTGVSTRVTNDNPGCRLVKESRICEVRPCGQPSYDSLKKGKKCTKTKKSQIPVKFTYAGCSSVKKYRPKYCGSCVDSRCCTPQQTRTIKVQFHCDDGETFTKNVMMIQSCRCSYNCPHGNEAYPNYQLFNDIHKFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKG6</accession>
    <name>A0A6I9XKG6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Very-long-chain enoyl-CoA reductase</fullName>
            <ecNumber evidence="6">1.3.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Synaptic glycoprotein SC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Trans-2,3-enoyl-CoA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">TECR</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="33">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="25">Involved in both the production of very long-chain fatty acids for sphingolipid synthesis and the degradation of the sphingosine moiety in sphingolipids through the sphingosine 1-phosphate metabolic pathway. Catalyzes the last of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme reduces the trans-2,3-enoyl-CoA fatty acid intermediate to an acyl-CoA that can be further elongated by entering a new cycle of elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Catalyzes the saturation step of the sphingosine 1-phosphate metabolic pathway, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2E)-hexadecenoyl-CoA + NADPH + H(+) = hexadecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:36143"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:36144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(2E,7Z,10Z,13Z,16Z)-docosapentaenoyl-CoA + NADPH + H(+) = (7Z,10Z,13Z,16Z)-docosatetraenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:39331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:73856"/>
            <dbReference type="ChEBI" id="CHEBI:76416"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:39332"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(2E,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + NADPH + H(+) = (7Z,10Z,13Z,16Z,19Z)-docosapentaenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:39467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:73870"/>
            <dbReference type="ChEBI" id="CHEBI:76461"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:39468"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(2E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + NADPH + H(+) = (8Z,11Z,14Z)-eicosatrienoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:39319"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:74264"/>
            <dbReference type="ChEBI" id="CHEBI:76412"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:39320"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a very-long-chain 2,3-saturated fatty acyl-CoA + NADP(+) = a very-long-chain (2E)-enoyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14473"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:83724"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="EC" id="1.3.1.93"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:14475"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>octadecanoyl-CoA + NADP(+) = (2E)-octadecenoyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35351"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:71412"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:35353"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.93" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013914501.1">
        <property type="nucleotide sequence ID" value="XM_014059026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKG6"/>
    <dbReference type="GeneID" id="106543114"/>
    <dbReference type="KEGG" id="tsr:106543114"/>
    <dbReference type="CTD" id="9524"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102758">
        <property type="term" value="F:very-long-chain enoyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17124">
        <property type="entry name" value="Ubl_TECR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000083">
        <property type="entry name" value="Trans-2,3-enoyl-CoA reductase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049127">
        <property type="entry name" value="TECR-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF31">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21696">
        <property type="entry name" value="TECR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 29" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="TECR-like N-terminal" evidence="31">
        <location>
            <begin position="5"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="30">
        <location>
            <begin position="155"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00057553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00072713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00078575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00081803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF21696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_013914501.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="35987" checksum="3912CD0E4369A0CB" modified="2020-10-07" version="1">MKYYEVEILDAKTREKLCFLDKVEPNATINEIKSLFTKSHPQWYPTRQSLRLDPKGKSLKDEDILQNLPVGTTATLYFRDLGAQISWVTVFLTEYAGPLLIYLMFYFRVPFIYGSRYDFTSSKYSVVHLACICHSFHYVKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPAYGHGQVKLALIIFLFCQLGNFSIHIALRNLRPAGSKARKIPFPTRNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFTLVGFIQMTIWAKGKHRSYLKEFRDYPPLRSPIVPFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM31</accession>
    <name>A0A6I9XM31_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cytochrome P450 2A13-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106541084</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911888.1">
        <property type="nucleotide sequence ID" value="XM_014056413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM31"/>
    <dbReference type="GeneID" id="106541084"/>
    <dbReference type="KEGG" id="tsr:106541084"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008392">
        <property type="term" value="F:arachidonate epoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019373">
        <property type="term" value="P:epoxygenase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11026">
        <property type="entry name" value="CYP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008067">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP2A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF180">
        <property type="entry name" value="CYTOCHROME P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01684">
        <property type="entry name" value="EP450ICYP2A"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013911888.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="56423" checksum="DCD3E4B771C928D6" modified="2020-10-07" version="1">MDLLGVGTLLLAVFLAGLLLLSSWQQMRRRGKMPPGPTPLPLIGNLLWIDRNNLPNSLIKLSEKYGPVYTLQLGPRRILVLCGYEAIKEALVDQGNEFRDRGQQATFDWIFQGHGVAFSNGEKAKQLRRFSIMTLRSFGVGKRSIEERILEEAHFLLEALRGTKGAPFDPTYFLSRSVSNVISSIAFGSRFDYEDQEFLSLLTAILEIFRFSSSARGQLLDIFFGIVQRLPGSHQMAFRRFSELKEFVAKKAKAHQETLDPNSPRDFIDCFLVKMLQEEKNPDSFFSMEELVATTLSIFFGGTETVSTTLRYGFLILLKHPEVEEKIHEEIDRVIGVNRTPSMEDRLQMPYTEAVIHEIQRFADILPLALPRRVTQDVQLRGYTIPKGTEVFPMLGTAMRDPKHFARPAEFDPQRFLDEKGQFQKNEAFIPFCIGKRYCFGEHLARAELFLFLTSILQHFRFKSAVPPEDIDLSPMLVGFGTIPRIYEISVIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMZ1</accession>
    <name>A0A6I9XMZ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Keratin, type I cytoskeletal 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cytokeratin-20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Keratin-20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106545313</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer of two type I and two type II keratins. Associates with KRT8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917304.1">
        <property type="nucleotide sequence ID" value="XM_014061829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMZ1"/>
    <dbReference type="GeneID" id="106545313"/>
    <dbReference type="KEGG" id="tsr:106545313"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF167">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="11">
        <location>
            <begin position="80"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="190"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="249"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013917304.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="50611" checksum="D7112653322F09DA" modified="2020-10-07" version="1">MARPGNFQNLSFFTKSGRHIPKDSHGMPAFFKQSWGPPSVYGGAGGSGTRISYGSNYDNTFGNKLQRSSSINELLLTFNEKTTMQNLNERLAAYLEKVHTLESSNSEFERRIREWHQKSSPIAKQDYNDFFKVIQELQDKITKARVDNTRIILQIDNSKLAADDFRVKYETELNLRMCVENDISGLQGLFDELTLVKTDLEHNIENLKEDLAYLKKTHEEEIAELRGRLRGHVSVELDAAPSIDLGQVMEKMRQQYEVMAEKNRQEAKDRFDKLIDQLNVEVTTTTQELETHRSEITDRKSVLQSLDIELRSQLNWKNERENALAETEALYRNKFAEIQMAIENVKARLKQLRHDMEQQAMEYSTLLDIKVKLEEEIATYRKLLEGENWRMTASDLETSKEAEQEKNKTLRIKTVIEEMVDGKVVSSQVDEREEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XND3</accession>
    <name>A0A6I9XND3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein FAM198B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FAM198B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GASK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917449.1">
        <property type="nucleotide sequence ID" value="XM_014061974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XND3"/>
    <dbReference type="GeneID" id="106545419"/>
    <dbReference type="KEGG" id="tsr:106545419"/>
    <dbReference type="CTD" id="51313"/>
    <dbReference type="OrthoDB" id="10011371at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029207">
        <property type="entry name" value="FAM198"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15905:SF1">
        <property type="entry name" value="GOLGI-ASSOCIATED KINASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15905">
        <property type="entry name" value="GOLGI-ASSOCIATED KINASE 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15051">
        <property type="entry name" value="FAM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917449.1"/>
        </source>
    </evidence>
    <sequence length="405" mass="46843" checksum="95E6BE6AE62EA957" modified="2020-10-07" version="1">MSFLVQTCQIINRLFCSLCSPRFLWSGKCSRTRRNLLLGTAFVIYLGFLVSQVGRVVPQYRKGLSKANSWNLQDVAQPRFSEIPMDGTWSFSDLEKSKMGTDNTSMLPNVVYITLRSKRRKPAKIRGTVRPKQRKKQVLPLQYGNKNSLDGIIQPKPFHGTSRNKRKGAEVPEKARQKQQQKEKQMAQNIKHWRPEMIRGLNVQTNVQESNIRMYSESPPSWLSKEDILSMRFMADSKINSIQEVPSQHGVLLVFGRTELDNKSVKEDLCSQGHCGIIKRPLEMSEVFAFHLDRILRLNRTLPTISRKLEFVQDDGPRPVILWDPSLAPTDNETHSSLRLTWGAYKKQLKEKCWQNGKIPKAEWGCTDIHYHEWSKMALLDFLLQIYHRIADLALSEHRNPLYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPR5</accession>
    <name>A0A6I9XPR5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GTPase-activating protein 31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ARHGAP31</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912703.1">
        <property type="nucleotide sequence ID" value="XM_014057228.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541711"/>
    <dbReference type="KEGG" id="tsr:106541711"/>
    <dbReference type="CTD" id="57514"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04384">
        <property type="entry name" value="RhoGAP_CdGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000002">
        <property type="entry name" value="rho GTPase-activating protein 32 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051576">
        <property type="entry name" value="PX-Rho_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15729">
        <property type="entry name" value="CDC42 GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15729:SF3">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="21"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="774"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1281"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1311"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="774"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1338"/>
            <end position="1348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912703.1"/>
        </source>
    </evidence>
    <sequence length="1461" mass="162486" checksum="5F8E7C7FC319A561" modified="2020-10-07" version="1">MKNKAGKVKLKRKGATNVFGCELSEYLESSGQDVPIVLKSCAEFIETHGIVDGIYRLSGVTSNIQKLRQEFGSDQCPDLTREVYLQDIHCVGSLCKLYFRELPNPLLTYELYKKFTEAVSCLSEKEQLAQIHNVIQELPPSHYRTLEYLSKHLALLASFSGMTNMHTRNLALVWAPNLLRSKEIEAEGCNGDAAFLEVRVQQLVIEFILNHVDEIFSDNVCIRPKDNEENKPVMKSLTLPSTPLPMKLVSLEEAQARSLSACHPARKERRENSLPEIVPATGSFYHTVLDLPDSKRKLSTKSKKWKSIFNLGRSGTEAKTKLSRNGSVFVRGQKLSEKATIRPAKSMDSLCSLPVEGEDEKGHFKRTITSGGFFVPMMKQRVGGPSSSFDLNKQDTEWDLKGAMKGAEGGAEDNAENHILRPTQMKPLPEQLKVFRPTEDPENEQTSPKMCRMFYTSNNETSNLGFHGTLFPLEASPRHQHKALNISEPFAVSVPLRVSAVISINSTPCRMPVKDKAAFSSLQESSFLGKGSAISSVPEGDNCDQLEHVTVKEEKKPESKSITDSTTEKIVIVKKLESVSIPQVTINTQETKSGGNNCSQPLEDKLPLEQSPLVMILASQQQTEKASQDQFGIKDMSEQISWPPELSIDKTEDNSHLKVVDDHSNTVMKPLWPDIQQELKIVESEEEFPCTGSALKTDEQGEFKPNLTSIASSETKSLVCSSLLQPTPPKEENIIGDPLAIPDILSKESKDVQLCNSETYRKQSGISFTSVKLQNSPCSKNCPQDDNTKNDLPSQSPNWKFPSQLKENTAFSTDKSSYSKENNLDQPKCESKNRLLQLEKKVETTAQLEKSSDIFSERDLTNSKIQCRAEVPWVKGGRINNQNSANRQEEDTWSDHVHSLELLEPWEDHQWVTSPLHSPTLKETQGKALQEFQPHNQGIAQSHLKKPCFSRSFSLDSKDFLKAHWAIEIPLANAHEEQLCGNLEQGMKEYSPGQWKESFQKAEAWLNCLKETSNSHEKHMKYLSHQAGFAATELPSGLEGLPSAACKALVKKKVPKDEKNQENMKLDSPSSQFNTEVSLLNSNCTGKHATNIQQNDRPGVPVMKGPCCTKELQIKKDEDIPCKGKPRPSSLNLDSVLPLASLFNFDNLSFPSSPRHFLCGQKEAKSSDSAPSLPVVCPSENKADLWGSHFDPQDLDLEYIMMAHATTGRRNSAPVSVSAVRTSFMVKMCQARAVPVIPPKIQYTQVPQPLQTQNPNCRISLASENKETEVKKSLIKPIQNAQGQLQLPKSPSLDGMGKENNNTLILDSAAARKAEPSFPNHNTTQDAPILRRKRTSEGETTGDTPQSSKMERPSGVSKPSYRSRPGRPQSLILFSPPFPIMDHPSSADSRVLLSPIRSPTQSPSPSPILNDLLGAAPEGVTLRNKMTIPKNGQRLETSTSCFYQPQRRSVILDGRSGRQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQK3</accession>
    <name>A0A6I9XQK3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">(E3-independent) E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="13">2.3.2.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">E2/E3 hybrid ubiquitin-protein ligase UBE2O</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Ubiquitin carrier protein O</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme E2 O</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Ubiquitin-conjugating enzyme E2 of 230 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Ubiquitin-protein ligase O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">UBE2O</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain). Interacts with UBR2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.24" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013916066.1">
        <property type="nucleotide sequence ID" value="XM_014060591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQK3"/>
    <dbReference type="GeneID" id="106544344"/>
    <dbReference type="KEGG" id="tsr:106544344"/>
    <dbReference type="CTD" id="63893"/>
    <dbReference type="OrthoDB" id="47801at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23837">
        <property type="entry name" value="UBCc_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000045">
        <property type="entry name" value="Ubiquitin conjugating enzyme E2 O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057732">
        <property type="entry name" value="SH3-A_UBE2O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057733">
        <property type="entry name" value="UBE2O-like_SH3-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057734">
        <property type="entry name" value="UBE2O-like_SH3-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057735">
        <property type="entry name" value="UBE2O-like_tSH3-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF15">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23048">
        <property type="entry name" value="SH3-A_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23043">
        <property type="entry name" value="SH3-B_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23044">
        <property type="entry name" value="SH3-C_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23046">
        <property type="entry name" value="tSH3-B_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="22">
        <location>
            <begin position="906"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="440"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="677"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="839"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1150"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="440"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="453"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1169"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1187"/>
            <end position="1201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013916066.1"/>
        </source>
    </evidence>
    <sequence length="1271" mass="139959" checksum="73A5955E34662ECF" modified="2020-10-07" version="1">MHGEDSDSEEEDGGGRGPGGGGPGGGGGERGGGGAGSSGGSESGGPGGGEENRGSPLRRGYVRVQWYPEGIKQHVKETKLKLEDRSVVPRDVVRHMGSRDSQCGTVVDVNIECAVKLVGTNCILYPVNSKDLQNIWPFMYGDCIAYDWWLGKVYDLKNQIILKLSNGARCSMSTEDAAKLYDVCPHGLFFDDSYGFYPGQVLIGPSKVFSNVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSISPPPSLITQENLSRVKRLGCFDHAERQLGERCLYVFPEKVEPAKITCECPERNCVLGEGSASKKMACPCFVSNVKPEKVRRLLKKQIVKIMSCSPDVQSTLDLPEGESAAAGDQQPAHLKANLKCGAEDSVSDITGLQDAKREHIDETRKSLSCEAAGLQLQPAFVLKREDGSLDYNLQSIEQDADDEAADDTDDTSSVTSSASSTASSQSGSGTGRKKSIPLSIKNLKRKHKKKKTKVSREFKPGDRVAVEVVTTMTSADVMWQDGTVEKNIRSNELFPVHHLDNNEFCPGDFVVDKRAQSSQDPGMYGVVQSGDHVGRTCVVKWFKLRPSGDDVELVGEEEDVSVYDIADHPDFRFRTTDFVIRIGNAEDGASIGENEPSVGQVARVDFSSKVEVVWADNSRTIVLPQHLYNIESEMDEETDLDSIDGSTSGASSDEWEDESDSWETDNGLMEDDHTKIEEVEPEELPVEEVKKVEEQVQGAVAMAVADLTTDKSGKEGTPKSFRELKEAIKILESLKNMTVEQLLTGYPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAMAEAEKKEEREVQTQTPVASEWPSETPVLCQQSGGRPGVTFTSAKGEVFSVLECAPDSHAFKKMEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALVKGPTRTPYEDGLFLFDIQLPNIYPAVPPLFRYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMMQLLRKPVEVFEQEIYEHFCCNGWRLIHRIESWVETNELVERSHDHAALAESSFVHSAGGILAESPVDSMGPLGEQELGAAGAASQLSDGKEELEDSGCGMSTVAASDTHQCSDSESMGQAREAHFARDVSDEGKASQDSATQPSVKPKKRRKSYRSFLPERSGYPDIGFPLFPLSKGFIKSIRGVLKLYRATLVEANIPEWTEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQT5</accession>
    <name>A0A6I9XQT5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">LOW QUALITY PROTEIN: zinc finger protein 721-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106546167</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918424.1">
        <property type="nucleotide sequence ID" value="XM_014062949.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546167"/>
    <dbReference type="KEGG" id="tsr:106546167"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002093">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000005">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000706">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000218">
        <property type="entry name" value="Zinc finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001298">
        <property type="entry name" value="zinc finger protein 23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000034">
        <property type="entry name" value="zinc finger protein 25"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001009">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000620">
        <property type="entry name" value="Zinc finger protein 263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000540">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000087">
        <property type="entry name" value="Zinc finger protein 354B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000022">
        <property type="entry name" value="zinc finger protein 383 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000052">
        <property type="entry name" value="zinc finger protein 546 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002134">
        <property type="entry name" value="Zinc finger protein 616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000292">
        <property type="entry name" value="zinc finger protein 619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000564">
        <property type="entry name" value="zinc finger protein 699"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000873">
        <property type="entry name" value="Zinc finger protein 841"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000344">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="38"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="16">
        <location>
            <begin position="48"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="17">
        <location>
            <begin position="204"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="264"/>
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</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR63</accession>
    <name>A0A6I9XR63_9SAUR</name>
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        <recommendedName>
            <fullName evidence="3 13">Arginase</fullName>
            <ecNumber evidence="3 13">3.5.3.1</ecNumber>
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    <gene>
        <name evidence="15" type="primary">ARG2</name>
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    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10 13">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="3 13"/>
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        <property type="nucleotide sequence ID" value="XM_014060781.1"/>
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    <dbReference type="OrthoDB" id="9992747at2759"/>
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        <property type="reaction ID" value="UER00270"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:arginine metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="entry name" value="Arginase"/>
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        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR014033">
        <property type="entry name" value="Arginase"/>
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    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
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        <property type="entry name" value="Ureohydrolase_dom_sf"/>
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    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
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    <dbReference type="NCBIfam" id="TIGR01229">
        <property type="entry name" value="rocF_arginase"/>
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    </dbReference>
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        <property type="entry name" value="ARGINASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782:SF4">
        <property type="entry name" value="ARGINASE-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
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    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
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    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
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    </dbReference>
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        <property type="entry name" value="ARGINASE_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0835">Urea cycle</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="145"/>
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        <ligand>
            <name>Mn(2+)</name>
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            <label>1</label>
        </ligand>
    </feature>
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        <ligand>
            <name>Mn(2+)</name>
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            <label>1</label>
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    </feature>
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        <location>
            <position position="149"/>
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        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
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    </feature>
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        <location>
            <position position="253"/>
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            <name>Mn(2+)</name>
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        <ligand>
            <name>Mn(2+)</name>
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            <label>1</label>
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    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013916256.1"/>
        </source>
    </evidence>
    <sequence length="356" mass="38657" checksum="05E88809790A8AC3" modified="2020-10-07" version="1">MAVRSSFSRLLQQAGSPLRVPRKLSHSVAIIGAPFSRGQKKRGVDHGPEAVRNAGLVGKLSSMGCQVYDFGDLNFTPVPNDELYNNLVKYPRSVGLASQLLADAVNGAVTAGHSCITIGGDHSLALGSISGHARQYPHLCVIWVDAHADINTPLTSQSRNLHGQPVSFLLRELQDKVPVLPGFSWLKPCLSQSDIVYIGLRDVDPAEHYILKNYGIQYFSMRHIDELGIQKVMEKTFDHLLGRGPRPIHLSFDIDAFDPSLAPATGTTVLGGLTYREGMFISEEIHNTGLLSAMDLVEVNPFLGASQEEVKATAGLAVDVIATSFGQTREGAHIVFENLPTPSSPDESENEEQVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTG0</accession>
    <name>A0A6I9XTG0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquitin carboxyl-terminal hydrolase 38</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">USP38</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917041.1">
        <property type="nucleotide sequence ID" value="XM_014061566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTG0"/>
    <dbReference type="GeneID" id="106545101"/>
    <dbReference type="KEGG" id="tsr:106545101"/>
    <dbReference type="CTD" id="84640"/>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02664">
        <property type="entry name" value="Peptidase_C19H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033840">
        <property type="entry name" value="USP38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049407">
        <property type="entry name" value="Usp38-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF710">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21246">
        <property type="entry name" value="Usp38-like_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="464"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013917041.1"/>
        </source>
    </evidence>
    <sequence length="1047" mass="116104" checksum="F4D394636BC402F8" modified="2020-10-07" version="1">MEKILEGLVSSSYPLPVKRMIVKRVVESAESPLSGPQCQAMFALSTRLILQGPDQFQRQVGRQVLEAYALHHRDDFEAFFTRDLVLALLHGAGGGGSPASPLFLAPAPLPAPGLGSHLDPGVLDYIQTGLRLLISCPAVLELFALLQREALRLVSERPPAPLCARLCQLLSDFPQCLPRGRKLALVFCQQLVRSIAHFQTQSTREAALRLYVSQVTQVSNLLRGIWKAEPDTLLPSLQELFAIISSTDTSEPSVALASLVQHIPLQMITVLIGSLTTDPNVKDASMTQALCRMIDWLSWPLAQHVETWVIALLKGLAAVQKFTILIDVTLLKIELVFNRLWFPLVRPGALAVLSHMLLSFQHSPEAFHLIVPHVVKLVTSVKSNGLPTSTTFLQQLSELMHCMMYHYSGFPELYEPILEAIKELFKPNEEKIKIILNQSAWTSQSSSLQSCLSRFSGKSETGKTGLINLGNTCYMNSVIQALFMATDFRRHVLSLNLNGCNSLMRKLQHLFAFLAHTQRDAYAPRLFFEASRPPWFTSRSQQDCSEYLRFLLDRLHEEERTLKVLSSSKLPEDMNEESLVQEVASKSQLFIKQSCSMKEERTLIEKMFRGKLQTSICCLNCKSTSHKEEAFTDLSLAFCPPVFFKNVGPPTVEHSSDEKDDSMEQVSARALSPVTEMPSGDSCEMVTNDGTLKDAPTDSNSENVTDPFICKTEVGDNLKKHVNEVTPSVTDLLNYFLAPEVLKGDNKYFCEKCASLQNAEKIMQIVEEPEYLILTLLRFSYDPKCHIRRKILDNVSLPLVLELPVNRTLSSLSTLSGIWSMSVEFSDTGENLAKKLKPSGAEEVGCPQLIPYVLSSVVVHSGISSESGHYYSYARNVANSRSSLGSFDQSQTMALAYPQSNLTAATEKDLDENEVSKEWFLFNDSRVTFTSFQSVQKITSRFPKDTAYVLFYKKQNSAGTISAKSASGLWINGDPPLQKELMDAITKDNKLYLQEQELSARTQALEASAASCSFRPNGFDDNDPPGSCGPTGGGGGGGFSTVGRLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTJ3</accession>
    <name>A0A6I9XTJ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Inhibitor of growth protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ING3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ING family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917086.1">
        <property type="nucleotide sequence ID" value="XM_014061611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTJ3"/>
    <dbReference type="GeneID" id="106545150"/>
    <dbReference type="KEGG" id="tsr:106545150"/>
    <dbReference type="CTD" id="54556"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16858">
        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15585">
        <property type="entry name" value="PHD_ING3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000103">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042020">
        <property type="entry name" value="ING3_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF103">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="4 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="359"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="128"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="286"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="136"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013917086.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="46439" checksum="7C1AFC71134BDD90" modified="2020-10-07" version="1">MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVNEFFMNAKKNKQEWREEQMTSIKKDYYKALEDADEKVQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILERRSLELDTPSQPLNNHHAHSHTPVEKRKHNPSSHHAAADHVPEKKFKSEALLSTLTSDASKENAQGCRNSNSSSSSNSAYNANSSQPLASYNLGSLSSGAGAGAITMAAAQAVQATAQMKEGRRTSSLKASYEAFKNNDFQLAREFALSRDTAGYTSSALASTLSQTLSSSTTDSRSGRKSKNNTKSSSQQSSSSSSSSSLSSCSSSSALAQELSQQTAAIPESESNSQVDWTYDPNEPRYCICNQVSYGEMVGCDNQDCPIEWFHYGCVGLTEAPKGKWYCPQCTAAMKRRGNRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTL2</accession>
    <name>A0A6I9XTL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Synaptosomal-associated protein 47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Synaptosomal-associated 47 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">SNAP47</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SVAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914024.1">
        <property type="nucleotide sequence ID" value="XM_014058549.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914033.1">
        <property type="nucleotide sequence ID" value="XM_014058558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTL2"/>
    <dbReference type="GeneID" id="106542732"/>
    <dbReference type="KEGG" id="tsr:106542732"/>
    <dbReference type="CTD" id="116841"/>
    <dbReference type="OrthoDB" id="10009801at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15888">
        <property type="entry name" value="SNARE_SNAP47N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000052">
        <property type="entry name" value="synaptosomal-associated protein 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000269">
        <property type="entry name" value="Synaptosomal-associated protein 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF1">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="135"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="374"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013914033.1"/>
        </source>
    </evidence>
    <sequence length="429" mass="47806" checksum="4384C1C178B7646F" modified="2020-10-07" version="1">MGDNIHIHAWSCSYYLNSGKQWVPGKLLLTPVSVKFKADKSGEILASFPLSSISEIKKESSALIFSSLTLLESNTKHWFSSLQPNRNVIFNVLEHFWREQLLSPGGARPQMACVQTSKGKELTGLLTGSEKRLEDTAKVLEHQGEQFDNIIKGLDKIESEMDVADRLLSELESPSWWPFGGKLPRAVSEAGKAKENPTASPSKNKDGVIMHIPVIITERTDAHVKPGSLTLLVSGLRICDANSQLLHWFDKREVDDVRVQSPYEICVRQRFIGKPDICYRLLSAKMPEAIPFLEVQFNKKIQFSEDALALARAKRASSPEEAGPSIWQAATGFMGSLVQAGATASSSSSSRGSGDHPEQHQAQLQKEKQVVSQSETQEIRQILRRLKCLALESETELERQDESLDAITSSVDRATMSIDRQNRRIRKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVJ2</accession>
    <name>A0A6I9XVJ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18 19">Zinc finger protein 319</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">ZNF319</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920168.1">
        <property type="nucleotide sequence ID" value="XM_014064693.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920169.1">
        <property type="nucleotide sequence ID" value="XM_014064694.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920170.1">
        <property type="nucleotide sequence ID" value="XM_014064695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVJ2"/>
    <dbReference type="GeneID" id="106547490"/>
    <dbReference type="KEGG" id="tsr:106547490"/>
    <dbReference type="CTD" id="57567"/>
    <dbReference type="OrthoDB" id="8895262at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000250">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001049">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001217">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001233">
        <property type="entry name" value="Zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001238">
        <property type="entry name" value="Zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000495">
        <property type="entry name" value="zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001134">
        <property type="entry name" value="Zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="416"/>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="444"/>
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            <begin position="473"/>
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            <begin position="501"/>
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    <feature type="domain" description="C2H2-type" evidence="15">
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            <begin position="529"/>
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        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013920168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013920169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013920170.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="66957" checksum="710D1FCB42C95F59" modified="2020-10-07" version="1">MSGLLWSDVSQSMSETWSQQPPPPQQAPHHVATATLPEHPIPPSAVTENPLGCAVYGILLQPEPSGLQHPSISASGGGEASPKCGICGHDLSHLSNPQEHQCLQGGHDRSFQCTQCMKIFHQATDLLEHQCLQVEQKPFVCGVCKMGFSLLTSLAQHHSVHNGNTAVKCSICEKTYKAAAAAVAAPEEPAPPQQPAALNRTPAEKPYSCSICQKPFKHLSELSRHERVHTGEKPYKCSLCDKSFSQSSHLVHHKRTHSAERPYKCTVCEKAFKHRSHLVRHMYAHSGEAHLFRCNVCELHFKESSELLQHPCTPSGERPFHCAACHKAFRRPSDLRQHERTHSTERPFQCDLCQMSFKQQYALMRHRRTHKAVAPAPSSEQEAAPLPFKCSLCEKGFVQPAHLLYHQHVHGIENLFKCNACQKGFSQSSELLRHRCVQSAERPFKCTACSKAYKRASALQKHQLASHCAEKPLRCTLCERRFFSSSEFVQHRCDPARERPLKCPDCQKRFKYASDLQRHRRVHTGEKPYKCPSCEKAFKQREHLNKHHGVHTREQHYKCMWCGERFLDLGLLQEHSAQHTSTEGTYTVAPCLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXB8</accession>
    <name>A0A6I9XXB8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Free fatty acid receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">G-protein coupled receptor 40</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106546461</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation. Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway. Mediates the anti-inflammatory effects of omega-3 polyunsaturated fatty acids (PUFAs) via inhibition of NLRP3 inflammasome activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918812.1">
        <property type="nucleotide sequence ID" value="XM_014063337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXB8"/>
    <dbReference type="GeneID" id="106546461"/>
    <dbReference type="KEGG" id="tsr:106546461"/>
    <dbReference type="OrthoDB" id="5961208at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045125">
        <property type="term" value="F:bioactive lipid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032024">
        <property type="term" value="P:positive regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070542">
        <property type="term" value="P:response to fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14983">
        <property type="entry name" value="7tmA_FFAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013312">
        <property type="entry name" value="GPR40-rel_orph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013313">
        <property type="entry name" value="GPR40_recept_FA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822:SF4">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01905">
        <property type="entry name" value="FATTYACIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01904">
        <property type="entry name" value="GPR40FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="22"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013918812.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="35010" checksum="22F06DF3E86BE61F" modified="2020-10-07" version="1">MGVWEIISLVVYGATILLGLPSNILALYIFYCRARVRLTPNLIYMINLCLSDLVFILFLPLKMLEKGKMDWTVPGFLCPLYNLIHFGTIYTSALFLTAVSAGRFLGAVYPLHYQAYKKPCYSCLVSVGIWALVGFHGVLLIALENSRGTNSSLFTGHSSSCYRNFSADQLALLAPVRLELSVVLFFLPLLITAFCYAGCIRVLVGSHLHLRKKRRAVRVAVATLSVFVLCFGPYNISHIVGFVYGEDVWWRTVALLPTACNAFLDPLIFYFLSSAMDDGIVQVWRSLRDKCSSIRMKIVLAHGKAKTQQGDGGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYL0</accession>
    <name>A0A6I9XYL0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">G protein-regulated inducer of neurite outgrowth 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">GPRIN3</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in neurite outgrowth.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919296.1">
        <property type="nucleotide sequence ID" value="XM_014063821.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919297.1">
        <property type="nucleotide sequence ID" value="XM_014063822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYL0"/>
    <dbReference type="GeneID" id="106546849"/>
    <dbReference type="KEGG" id="tsr:106546849"/>
    <dbReference type="CTD" id="285513"/>
    <dbReference type="OrthoDB" id="10049175at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026646">
        <property type="entry name" value="GPRIN2-like/GPRIN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032745">
        <property type="entry name" value="GRIN_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15718:SF6">
        <property type="entry name" value="G PROTEIN-REGULATED INDUCER OF NEURITE OUTGROWTH 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15718">
        <property type="entry name" value="G PROTEIN-REGULATED INDUCER OF NEURITE OUTGROWTH C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15235">
        <property type="entry name" value="GRIN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G protein-regulated inducer of neurite outgrowth C-terminal" evidence="3">
        <location>
            <begin position="648"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="715"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="717"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013919296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013919297.1"/>
        </source>
    </evidence>
    <sequence length="766" mass="82819" checksum="68F6A8B534C60240" modified="2020-10-07" version="1">MGTVQDPLRSTKLSLVTAEESPKHLCKQASIESSSNGYPFTAKDQLSGGCLLEVNSGRETNVPKSDWHCEYDIKHPNILSPGSFSFSKEGSMAAPEPVSYAKQEGSRAKVQMSVPEAHPSEHGEVKVPLTQEANQNIPSSGGQSCNIIDKATVLPGGDLDITESKSKDYSIVPSLPIYSNIQQASPVGKDNMCEVIISETSPVLVSSDEMGLNPKAPVGVETELGNTNDANVCPKTEPQTASEFSNLETSCTGSEEPTAKVVPNLSKFKDTGTMTVKPENKSVEAETISRTHQDAGVQAVASVESRSASTSPSILTAFLRENMSPEAKQDQDQLHVIYTGARGKEQPEIIGDFAATVQTPSPIGIMHKVRVQIPPARENPLGSPNVRLQENLTNTHDPICAALLDKAARVCSSDDSQETLAKRTEVQTTGVAKNRGDVPQQLSDNSVLQKARPVSQISLSSSHPPVSTQHPANFETKLPPSFATPTIDSRYQPADPSSFREHPLPLCPRTSEQAKAIVIGMNTERTYQEKQLQIKAVDNLETALPSFPIAMHLKREGKVHLISEEQKINMTGSIIGSRTMSAPVVAESKQDTVPGDAKTETLAGKNPISKLSRSFGAAIGIQSDKIQKGLVLDVPAARTSSSPTPRLGEKKKGPKAFAAEAKMHLKQSKRVRDVVWDEQGMTWEVYGASLDPESLGIAIQNHLQRQIREHEKLIKAQSPQARKSISSDTSSNKKLKGRHHSVFQSMLQNFRRPNCCVRPGASSVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0P6</accession>
    <name>A0A6I9Y0P6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Short transient receptor potential channel 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TRPC1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910315.1">
        <property type="nucleotide sequence ID" value="XM_014054840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0P6"/>
    <dbReference type="GeneID" id="106539916"/>
    <dbReference type="KEGG" id="tsr:106539916"/>
    <dbReference type="CTD" id="7220"/>
    <dbReference type="OrthoDB" id="2373987at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000088">
        <property type="entry name" value="short transient receptor potential channel 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000266">
        <property type="entry name" value="Transient receptor potential cation channel subfamily c member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013555">
        <property type="entry name" value="TRP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005457">
        <property type="entry name" value="TRPC1_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00870">
        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF56">
        <property type="entry name" value="SHORT TRANSIENT RECEPTOR POTENTIAL CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08344">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01097">
        <property type="entry name" value="TRNSRECEPTRP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01642">
        <property type="entry name" value="TRPCHANNEL1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01420">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="7" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="575"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Transient receptor ion channel" evidence="19">
        <location>
            <begin position="181"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013910315.1"/>
        </source>
    </evidence>
    <sequence length="781" mass="90190" checksum="886DBB1700B2FF26" modified="2020-10-07" version="1">MAALYQSADPSASASPNKLLALKDVRQVKEETTLDEKLFLLACDKGDFYMVKKLLEENNSGDMNINCVDVLGRNAITISIENENLDILQLLLDYGCQSTDALLVAIDSEVVGAVDILLNHRPKRSSRPTIVKLMERMQNPEYSTTMDVAPVILAAHRNNYEILTMLLKQDVSLPKPHAVGCECTLCTAKNKKDSLRHSRFRLDIYRCLASPALIMLTEEDPILRAFELSADLRELSLVEVEFRNDYEELAQQCKTFAKDLLAQARNSRELEVILNHTASDEHVDKRGLREERMNLSRLKLAIKYNQKEFVSQSNCQQFLNTVWFGQMAGYRRKHTFKKILTVLTVGILWPLLSFSYLLAPRSQIGRIIHTPFMKFIIHGASYFTFLLLLNLYSLVYNEDKKNTMGPALERIDYLLILWLIGMVWSDVKRLWYDGLEDFLEESRNQLSFVMNSLYLATFALKIVAHKKFHDFAERKDWDAFHPTLVAEGLFAFANVLSYLRLFFMYTTSSILGPLQISMGQMLQDFGKFLGMFLLVLFSFTIGLTQLYDKGFTVHEEKDCAGIFCEQQSNDTFHSFIGTCFALFWYIFSLAHVAIFVTRFNYGEELQSFVGAVIVGTYNVVVVIVLTKLLVAMLHKSFQLIANHEDKEWKFARAKLWLSYFDDKCTQPPPFNIIPAPKTICYIFNSLSKWISSHTSSGKVKRQNSLKEWKSLKQKRDENYQKVMCCLVHRYLTSMRQRMQSTDQATVENLNELRQDLSKFRNEIRDLLGFRTSKYAIFYQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0T0</accession>
    <name>A0A6I9Y0T0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calmin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Calponin-like transmembrane domain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CLMN</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916563.1">
        <property type="nucleotide sequence ID" value="XM_014061088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0T0"/>
    <dbReference type="GeneID" id="106544729"/>
    <dbReference type="KEGG" id="tsr:106544729"/>
    <dbReference type="CTD" id="79789"/>
    <dbReference type="OrthoDB" id="10017054at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21245">
        <property type="entry name" value="CH_CLMN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000057">
        <property type="entry name" value="Calmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000063">
        <property type="entry name" value="Calmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047826">
        <property type="entry name" value="CLMN_CH_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052403">
        <property type="entry name" value="LINC-complex_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535:SF7">
        <property type="entry name" value="CALMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535">
        <property type="entry name" value="MUSCLE-SPECIFIC PROTEIN 300 KDA, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="848"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="32"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="187"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="151"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="522"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="643"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="660"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="697"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="709"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="797"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013916563.1"/>
        </source>
    </evidence>
    <sequence length="873" mass="98612" checksum="26E09D23206911D6" modified="2020-10-07" version="1">MAGSEWDWFQREELIGQISDIRVQNLQVERENVQKRTFTRWMNLHLEKCSPPLEVKDLLVDIKDGKILMALLEVLSGQNLLHEYKSSTHRIFRLNNIAKALKFLEDSNVKLVSIDASEIADGNSSMVLGLIWNIILFFQIKELTGNLNRNSSSSSLSSGPSGPDSDTSHPSTPNVERNMSVSVKDQRKAIKALLTWVQRKTRKYGVAIQDFTSSWRSGLAFLAVIKAIDSSLVDMKQALEKSPRENLEDAFCTAQVYLGIPRLLEPEDIMIDSPDEQSIMTYVAQFLEHFPELEAEDFSDLDKEFPIESAYVHIKEIPEEEKQEKVLLLNENGEHIYPIHLVENQSPLHQLDGFTLPLDEENQNFHATSTESNDSLADYMDSSTNEPQRPRSLPIRSVQISPIAKFANQFVNQSEVNQVNLSASIKSDDLPSTVFIYHKDSAEPVDSDEFDSDIQKSSSEIKESPESNICSLPWTSHLSKNVSEHLTNEPEYVIPISEEQEVVQKYILEMLNQEIISKLTENDEPMEQSSNLKEIPSTNQEIDGHDVKDNSEATYTPEYEPISNTSKSLNAAIEESMMTPNPSKVSIIPHDLFYYPHYDVSISALLDAFTTSTSDTDLSLLENSKICSEILVDCLAENEPLSLSTEGNIPKQDLPSNMKDVPKKKDNKSPRKQKRDSQSHTKFSFGTAAVVGKNTVPHKSNNMATTENDSAHPDVIKENQSDTAAEERKLPEERSKKKEKRKNKAIFQGESPQSSKPGFTRLIDKLEEESIDHLALARMSYSDPNIVYQITTTDSTIKESGSPGVQKSTNPSEYPTPAIRKRKDLEEEDILEKELLCMTSSRTDQPELFYFIIFLWAIVYCLLLLPQLISSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y154</accession>
    <name>A0A6I9Y154_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cyclic AMP-dependent transcription factor ATF-1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106540027</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910475.1">
        <property type="nucleotide sequence ID" value="XM_014055000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y154"/>
    <dbReference type="GeneID" id="106540027"/>
    <dbReference type="KEGG" id="tsr:106540027"/>
    <dbReference type="OrthoDB" id="5970722at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990589">
        <property type="term" value="C:ATF4-CREB1 transcription factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14690">
        <property type="entry name" value="bZIP_CREB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000003">
        <property type="entry name" value="cAMP-responsive element modulator isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003102">
        <property type="entry name" value="CREB1-like_pKID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001630">
        <property type="entry name" value="Leuzip_CREB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879">
        <property type="entry name" value="CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879:SF2">
        <property type="entry name" value="CYCLIC AMP-DEPENDENT TRANSCRIPTION FACTOR ATF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02173">
        <property type="entry name" value="pKID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00041">
        <property type="entry name" value="LEUZIPPRCREB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50953">
        <property type="entry name" value="KID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="KID" evidence="9">
        <location>
            <begin position="77"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="252"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013910475.1"/>
        </source>
    </evidence>
    <sequence length="310" mass="33981" checksum="C92565F9EABAE3FF" modified="2020-10-07" version="1">MDEVPKSSNNNLSTSPQVASLQGTTLQEAQLSHVSQQMSLRGPTPLTVVHLSGDQVQGVIHTAQTSSVIHSPQGQSVQVSLSENDDSQDSSDSMDSSQKARGLLARRPSYRKIFNDLSSEDTRDRKGDESNSVVTSVPVTTPVYQTSTGQYSFEVAIAPNGPLQLSSPGAESVQGLQTFTNAAGTPLLQYVQTSDGQQILVPSNQVVVQTTSGDMQTYQLRRTPTTTSLPQTVVMTTPVSLTSQSTKSDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELRTLKDLYCHKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y160</accession>
    <name>A0A6I9Y160_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell cycle exit and neuronal differentiation protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CEND1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920126.1">
        <property type="nucleotide sequence ID" value="XM_014064651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y160"/>
    <dbReference type="GeneID" id="106547462"/>
    <dbReference type="KEGG" id="tsr:106547462"/>
    <dbReference type="CTD" id="51286"/>
    <dbReference type="OrthoDB" id="9428119at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021686">
        <property type="term" value="P:cerebellar granular layer maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021702">
        <property type="term" value="P:cerebellar Purkinje cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021933">
        <property type="term" value="P:radial glia guided migration of cerebellar granule cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020162">
        <property type="entry name" value="Cend1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36683">
        <property type="entry name" value="CELL CYCLE EXIT AND NEURONAL DIFFERENTIATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36683:SF1">
        <property type="entry name" value="CELL CYCLE EXIT AND NEURONAL DIFFERENTIATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15677">
        <property type="entry name" value="CEND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920126.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="13963" checksum="31EBEF2D56589487" modified="2020-10-07" version="1">MSRMESKESTRSGNKTDTKSASSAKSEKPHSGPAASTDKKEAPKEQPTPAATTPAKKGATAPSEAAMLNDHSNLKPTAVPAAEGPEATANSEHKESSAEESPGSTVFENIKPLLIFGGAAVAAIAVIIGVAILARKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1I9</accession>
    <name>A0A6I9Y1I9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATPase WRNIP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">WRNIP1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920316.1">
        <property type="nucleotide sequence ID" value="XM_014064841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1I9"/>
    <dbReference type="GeneID" id="106547614"/>
    <dbReference type="KEGG" id="tsr:106547614"/>
    <dbReference type="CTD" id="56897"/>
    <dbReference type="OrthoDB" id="10265467at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:enzyme activator activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000137">
        <property type="entry name" value="Replication-associated recombination protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051314">
        <property type="entry name" value="AAA_ATPase_RarA/MGS1/WRNIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779:SF7">
        <property type="entry name" value="ATPASE WRNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779">
        <property type="entry name" value="WERNER HELICASE-INTERACTING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00734">
        <property type="entry name" value="ZnF_Rad18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51908">
        <property type="entry name" value="ZF_UBZ4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBZ4-type" evidence="8">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013920316.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="38172" checksum="27DBCD7136C83766" modified="2020-10-07" version="1">MEGGTAQEEPLVSCPVCLMELPSARINSHLDSCLQLQGEGEDLFTAEVGGRSHSAPKPPAGAAAAPPNPQQPSSKKIRLSRSPERRNQGEEQPGNDLGSPPVFPLFHRGRNPGGSPAGAPRGAATSPNPGKGEERPGETPERAPGASEPPLRGESLAQKLEGKPLADKLRPSHLGDYVGQNRALGKETLLRSLLEAHEVPSLILWGPPGCGKTTLAHIIASSSKKNGTRFVTLSATSAKTSDVRDVIFQAQNEKRLCKRKTILFIDEIHRFNKSQQDTFLPHVECGTITLIGATTENPSFQVNSALLSRCRVIVLEKLSVEAVEAILMRAVKSLGIQVLSQDEGLTSSLNANHSQGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1J3</accession>
    <name>A0A6I9Y1J3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rho-related BTB domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920782.1">
        <property type="nucleotide sequence ID" value="XM_014065307.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="6020506at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001999">
        <property type="entry name" value="Rho-related BTB domain-containing protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920782.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="13891" checksum="02B14BA27EA6D1B7" modified="2020-10-07" version="1">MAARNKSADKRKAKMNRSLSQLMDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y239</accession>
    <name>A0A6I9Y239_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kaptin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KPTN</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013910855.1">
        <property type="nucleotide sequence ID" value="XM_014055380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y239"/>
    <dbReference type="GeneID" id="106540305"/>
    <dbReference type="KEGG" id="tsr:106540305"/>
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    <dbReference type="OrthoDB" id="10267127at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140007">
        <property type="term" value="C:KICSTOR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029982">
        <property type="entry name" value="Kptn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15435">
        <property type="entry name" value="KICSTOR COMPLEX PROTEIN KAPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15435:SF2">
        <property type="entry name" value="KICSTOR COMPLEX PROTEIN KAPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013910855.1"/>
        </source>
    </evidence>
    <sequence length="102" mass="11379" checksum="570D0D67BD929DBE" modified="2020-10-07" version="1">IPVDAEIVSIDTFNKPSPKRGLVVGITFIKDSGDKASPFLNIYCDYEPGSEYNLDSIAQSCLNLELQFTPFQLYHAEVQVADRPETVFLLSGNDPAIHLYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y268</accession>
    <name>A0A6I9Y268_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pleckstrin homology domain-containing family O member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PLEKHO1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910885.1">
        <property type="nucleotide sequence ID" value="XM_014055410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y268"/>
    <dbReference type="GeneID" id="106540333"/>
    <dbReference type="KEGG" id="tsr:106540333"/>
    <dbReference type="CTD" id="51177"/>
    <dbReference type="OrthoDB" id="6358316at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036195">
        <property type="term" value="C:muscle cell projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901739">
        <property type="term" value="P:regulation of myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043448">
        <property type="entry name" value="PKHO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15871">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15871:SF1">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY O MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="159"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013910885.1"/>
        </source>
    </evidence>
    <sequence length="385" mass="42641" checksum="BFB4782BAF9ED96C" modified="2020-10-07" version="1">MQILGTVSMKENITNAANAHKAAVKLRDEKNIQEAFDLNDYEKCEELRKSKSRSKKNYSKFSLAHSKEPGNTAPNLVFLAVSPEEKESWINVLNSAIIRAKNRILDEVTVEEESFLAHPTRNRAKIQHSRRPPTRGHLMAVASTSTSDGMLTLDLIQEEEASPEDPCEKSLRVDKSAAHFSPGRRRSSDSEEAKDSEKASSLPRRKDLSGEKVSAHREPFGRGTMYTPQVPKKLSHLEKNKCASMEEILSCWDSPSAGQPALRTGLKDPAASPEPEQLARIQALVALQLEKTQGLLAEARGYGGEGLRKAQELGSACAPSSPKLDSAATLQEIQRLLGEASSSWSQAQKVLQEVRELRSLYQQLRLSRPAPPQSLLLPQYRKSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2J7</accession>
    <name>A0A6I9Y2J7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EMC2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EMC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917293.1">
        <property type="nucleotide sequence ID" value="XM_014061818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2J7"/>
    <dbReference type="GeneID" id="106545306"/>
    <dbReference type="KEGG" id="tsr:106545306"/>
    <dbReference type="CTD" id="9694"/>
    <dbReference type="OrthoDB" id="124397at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="EMC2 TPR-like" evidence="5">
        <location>
            <begin position="77"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013917293.1"/>
        </source>
    </evidence>
    <sequence length="224" mass="26533" checksum="EA05B4C33FBE88BC" modified="2020-10-07" version="1">MAKVSEMYDVTWEEMRDKMRKWREENYRNSEQIVEVGEELINEYAAKLGDDIWIIYEQVMIAALDCNRDDLATYCLQELRRQFPGSHRVKRLTGMKFEAMERAARKRKIAIRKAQGKNTEAIRELNEYLEQFVGDQEAWHELAELYINEHDPIKLLITRVKYTQGGLENLELSRKYFAQALKLNNRNMRALFGLYMYAGRSKKETKYSLKAVEDMLEALQITQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y376</accession>
    <name>A0A6I9Y376_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription factor HES-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HES2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921211.1">
        <property type="nucleotide sequence ID" value="XM_014065736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y376"/>
    <dbReference type="GeneID" id="106548374"/>
    <dbReference type="KEGG" id="tsr:106548374"/>
    <dbReference type="CTD" id="54626"/>
    <dbReference type="OrthoDB" id="6085656at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11463">
        <property type="entry name" value="bHLH-O_HES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000009">
        <property type="entry name" value="Transcription factor HES-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="135"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013921211.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="19571" checksum="8699D21220FF4080" modified="2020-10-07" version="1">MSPGRKTGEPSELRKSLKPLMEKRRRARINCSLGQLKTLILPLVGKDNSCYSKLEKADILEMTVQFLKDLPTSCGTLPGSVDGYCEGYHTCLSHFATLIPKYNLLSPEACNQLLARLQQTFKDCAWHHNRTNVAKDLASTPRAETPLGPPAGQRDQEDFWGSSPPAQPPPLWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y385</accession>
    <name>A0A6I9Y385_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Neurobeachin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106548394</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921226.1">
        <property type="nucleotide sequence ID" value="XM_014065751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y385"/>
    <dbReference type="GeneID" id="106548394"/>
    <dbReference type="KEGG" id="tsr:106548394"/>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF62">
        <property type="entry name" value="NEUROBEACHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="2">
        <location>
            <begin position="67"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013921226.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="15334" checksum="847459C293C8389C" modified="2020-10-07" version="1">MFSEIRAVFSRRYLLQNTALEVFMANRTSVMFNFPDQATVKKVVYSLPRVGVGTSYGLPQARRISLATPRQLYKSSNMTQRWQRREISNFEYLMFLNTIAGRTYNDLNQYPVFPWVLTNYESEELDLTLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4N6</accession>
    <name>A0A6I9Y4N6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenylate kinase 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912455.1">
        <property type="nucleotide sequence ID" value="XM_014056980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4N6"/>
    <dbReference type="GeneID" id="106541506"/>
    <dbReference type="KEGG" id="tsr:106541506"/>
    <dbReference type="OrthoDB" id="9394182at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912455.1"/>
        </source>
    </evidence>
    <sequence length="194" mass="22135" checksum="9D3C34D00CC8C61F" modified="2020-10-07" version="1">MTMVMIIESAEFMEGSFGIERGQPEQDDSSESKRKESSGETMKFVVKSDDSKEPEGSEESKTESTEPLESEKKSVSQYEHLKSMASELGVTMPEYPEDGFPDAVEIEPLRNKLTQFQNNWQKLEQHITESLLVQSTELEIDGQTPEMLLNKTVQAMQQTFKYHGWELLPEDEDEEQEDLQAEAEAQAEEEEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y509</accession>
    <name>A0A6I9Y509_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">T-box transcription factor TBX2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106546930</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919398.1">
        <property type="nucleotide sequence ID" value="XM_014063923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y509"/>
    <dbReference type="GeneID" id="106546930"/>
    <dbReference type="KEGG" id="tsr:106546930"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20188">
        <property type="entry name" value="T-box_TBX2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000003">
        <property type="entry name" value="T-box transcription factor TBX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022582">
        <property type="entry name" value="TBX2/3_TAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002070">
        <property type="entry name" value="TF_Brachyury"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF181">
        <property type="entry name" value="OPTOMOTOR-BLIND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12598">
        <property type="entry name" value="TBX2-3_TAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00938">
        <property type="entry name" value="BRACHYURY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="100"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919398.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="52159" checksum="A5E4DCC9E151A089" modified="2020-10-07" version="1">MRDPAFSGAAMAYHPFHAPRPADFPMSAFLAAAQPSFFPALALPPAALTKPLPDPSLAGAAAEAGLHVSALGHHHQAAHLRSLKSLEPEEEVEDDPKVTLEAKDLWDEFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLPSLRMYEDPCKQDRDGGESDASSCEPAPARESLHSPLATTPSPLRVHRNSREDKGSDQELEKGSGVKRTSPDLGPATPPHNRSPGLQADERSKERAKASEASKDGGGEAALFTPLALEKEKLEGRRKEPLLQPPQEGSKKELPGDCAPVGCSSKEAFAPLMVQTDSPPHLNPSHLQSLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y675</accession>
    <name>A0A6I9Y675_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MYC</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913760.1">
        <property type="nucleotide sequence ID" value="XM_014058285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y675"/>
    <dbReference type="GeneID" id="106542510"/>
    <dbReference type="KEGG" id="tsr:106542510"/>
    <dbReference type="CTD" id="4609"/>
    <dbReference type="OrthoDB" id="5964374at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11458">
        <property type="entry name" value="bHLHzip_c-Myc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000019">
        <property type="entry name" value="Myc proto-oncogene protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003327">
        <property type="entry name" value="Myc-LZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050433">
        <property type="entry name" value="Myc_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002418">
        <property type="entry name" value="Tscrpt_reg_Myc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012682">
        <property type="entry name" value="Tscrpt_reg_Myc_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45851">
        <property type="entry name" value="MYC PROTO-ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02344">
        <property type="entry name" value="Myc-LZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01056">
        <property type="entry name" value="Myc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001705">
        <property type="entry name" value="Myc_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00044">
        <property type="entry name" value="LEUZIPPRMYC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="344"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR001705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913760.1"/>
        </source>
    </evidence>
    <sequence length="429" mass="47698" checksum="DA3F3CB570FC163A" modified="2020-10-07" version="1">MPLTVPSLSYPSRAYDYDYDSIQPYFYYEDEEENFYLSGHHRGCELQPPAPSEDIWKKFELLPTPPLSPSRRSSSFPSHADQLEMVTELLGSEVVNQSFICDPDDDAFVKSIIIQDCMWSGGSAAAKLQKVVSEKLASYQAARRDGAGSPLSLRSGNSSVSGLPSSSSHSSASSPSSSSSYLQDLGASAAECIDPSVVFPYPLGEKTPKAVEASASSPDPCPASLLGQDTPPTTSSDSEEEEEEEQEEEEEIDVVTVEKRQPSARKSESVGHTSGGHSKPPHSPLVLKRCHVPIHQHNYAAPPSTRHEYSASKRLKLDNGRVLKQISNNRKCSSPRTSDSEENDKRRTHNVLERQRRNELKLSFFALRDQIPEVANNEKAPKVVILKKATEYVLSIQSEEHRLLAEKEHLRKRHEQLKNKLQQLRNSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6H8</accession>
    <name>A0A6I9Y6H8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein NDNF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NDNF</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Secretory protein that plays a role in various cellular processes. Acts as a chemorepellent acting on gonadotropin-releasing hormone (GnRH) expressing neurons regulating their migration to the hypothalamus. Also promotes neuron migration, growth and survival as well as neurite outgrowth and is involved in the development of the olfactory system. May also act through the regulation of growth factors activity and downstream signaling. Also regulates extracellular matrix assembly and cell adhesiveness. Promotes endothelial cell survival, vessel formation and plays an important role in the process of revascularization through NOS3-dependent mechanisms.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914045.1">
        <property type="nucleotide sequence ID" value="XM_014058570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6H8"/>
    <dbReference type="GeneID" id="106542751"/>
    <dbReference type="KEGG" id="tsr:106542751"/>
    <dbReference type="CTD" id="79625"/>
    <dbReference type="OrthoDB" id="9872501at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019326">
        <property type="entry name" value="NDNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045805">
        <property type="entry name" value="NDNF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055271">
        <property type="entry name" value="NDNF_Fn(III)_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056225">
        <property type="entry name" value="NDNF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14619">
        <property type="entry name" value="NEURON-DERIVED NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14619:SF1">
        <property type="entry name" value="PROTEIN NDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10179">
        <property type="entry name" value="NDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19433">
        <property type="entry name" value="NDNF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24354">
        <property type="entry name" value="NDNF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026702850" description="Protein NDNF" evidence="9">
        <location>
            <begin position="22"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="176"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="447"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914045.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="65098" checksum="3589790042518897" modified="2020-10-07" version="1">MLLIRCSIFYLFLLLPLSSWMQKLPTRDEELFQMQIQDKSFFHDSSVLPDGAEISGYLFRGTPKRFFFVVEEDNTPFAVTVTPCDTPLEWKLSLQDLPEESSGEGSGELEPLEQQNQQIIREEDTELFSYRGNDVEYFVSSTSPSGLYQLELWSTEKDTNFKVYASTTPESDQPYPELPYDPRVDVTSFGRTTVSLAWKPSPTASVLKQPIQYCVVINKEHNFKSLCAVETKQTADDTFMKAPKPGLDFSPFDFAHFGFSSNSNFGKERSFLKATPKLYSKLYSKPKVDLEKICIGNKNIFTVSELKPGTQYYFDIFAVNVNTNMSTAYVGTFARTKEEAKQKTVDLKDGKVTDVFVKGKGTIFLRFAPVSSHQKVTFLVHSCLNDIQMQIKRDGKLRLSQNVEGVHQFQLRGKPKAKYLVRLKGNKKGASILKFLATTRPNKQSFPSLPEDTRIKAFDKLRTCSSVTVAWLGTQERNKFCIYKKEVDDNYNEEKKKREQNQCLGPDLRKKSEKVLCKYFHSQNLQKAVTTETIKGLKPGKSYVLDVYVIGHSGYSVKYQSKLVKTRKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7V3</accession>
    <name>A0A6I9Y7V3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ski oncogene</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Proto-oncogene c-Ski</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SKI</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915825.1">
        <property type="nucleotide sequence ID" value="XM_014060350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7V3"/>
    <dbReference type="GeneID" id="106544167"/>
    <dbReference type="KEGG" id="tsr:106544167"/>
    <dbReference type="CTD" id="6497"/>
    <dbReference type="OrthoDB" id="3938623at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21083">
        <property type="entry name" value="DHD_Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000002">
        <property type="entry name" value="Putative ski oncogene"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.20:FF:000002">
        <property type="entry name" value="SKI-like oncogene a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.20">
        <property type="entry name" value="Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014890">
        <property type="entry name" value="c-SKI_SMAD4-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047315">
        <property type="entry name" value="DHD_Ski"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003380">
        <property type="entry name" value="SKI/SNO/DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023216">
        <property type="entry name" value="Tscrpt_reg_SKI_SnoN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005:SF15">
        <property type="entry name" value="SKI ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005">
        <property type="entry name" value="SKI ONCOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08782">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01046">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="c-SKI SMAD4-binding" evidence="5">
        <location>
            <begin position="220"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="442"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="724"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="467"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="505"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="724"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013915825.1"/>
        </source>
    </evidence>
    <sequence length="746" mass="82820" checksum="1017007B3D48C8E3" modified="2020-10-07" version="1">METVPRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQELYKRETAKEAASVASSSSSSVAGPEAPLPPLPAIPPPPAPVMPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINAVCDELHVYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPSRCKKEMPSGTAANTLELELTEGSFRIYHECFGKCKGLLVPELYSSPGAPCIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYLLLSPDYASKEDKARLSQCLDEMKEKFDYSHRYKRKAPRVPSDPLVPKKLKTDDALQSPLLGDKDKQSSWLRSLSNSSSKNIGCLHPRQRLSAFRPWSPAVSASEKDVANHIPTLIRDSCYSYKSFENSVAPNVALAPPPQPKIVSNPLCPPAALQNAEPLSSPPQLRKRKHVAEHTVVPEPPPPAAAVPTVVPPVPAAEEEKESEAEIEVETREEFTSSLSSLSSPSFTSSGSAKDMNSPSLQVQPAAGNAVFEVSSHAEPQNGPNGLEAELEHLRQALDGGLDTKEAKEKFLHEVVKMRVKQEEKLNAALQAKRSLHQELEFLRVAKKEKLREATEAKRNLRKEIERLRAENEKKMKEANESRIRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLLHEREARENLEKAVKELQEQLWSKHNDLPNSAHTPKDPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8V5</accession>
    <name>A0A6I9Y8V5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Phosphatidylinositol-4,5-bisphosphate 4-phosphatase</fullName>
            <ecNumber evidence="4 12">3.1.3.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TMEM55A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25674"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Late endosome membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 12">Lysosome membrane</location>
            <topology evidence="3 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.78" evidence="4 12"/>
    <dbReference type="RefSeq" id="XP_013917585.1">
        <property type="nucleotide sequence ID" value="XM_014062110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8V5"/>
    <dbReference type="GeneID" id="106545515"/>
    <dbReference type="KEGG" id="tsr:106545515"/>
    <dbReference type="CTD" id="55529"/>
    <dbReference type="OrthoDB" id="9939933at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034597">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019178">
        <property type="entry name" value="PtdIns-P2-Ptase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21014">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21014:SF5">
        <property type="entry name" value="TYPE 2 PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09788">
        <property type="entry name" value="Tmemb_55A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0967">Endosome</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013917585.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="29165" checksum="AC3A540FCAFC7F9E" modified="2020-10-07" version="1">MAADGMDERSPLLSAPGSGNVTPTAPPYFPDSSPRAELPPPYTAIASPDATAIPIINCRVCQSAIHLEGKLHQHVVKCTDCNEATVNGFTSFSFYNMQFMVIKNAIWGMSNFSTFNFQILEYDIFTSDSRRIINLGPVMLIPEEQPAQPAIPAQPEGTRVVCGHCGNTFLWMELRFNTLAKCPHCKKISSVGCALPRRRCCAYITIGMIFIFIGVGLTVGTQDFARTFHATYVSWAVAYLFGLVCLVRACYWGAVKVSYPEHSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9H8</accession>
    <name>A0A6I9Y9H8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="32">Probable carboxypeptidase PM20D1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">PM20D1</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="32">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction. Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + L-phenylalanine = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-phenylalanine + H2O</text>
            <dbReference type="Rhea" id="RHEA:51312"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:134022"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:51313"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:51314"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(9Z)-octadecenoate + glycine = N-(9Z-octadecenoyl)glycine + H2O</text>
            <dbReference type="Rhea" id="RHEA:51316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:133992"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:51318"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-phenylalanine + (9Z)-octadecenoate = N-(9Z-octadecenoyl)-L-phenylalanine + H2O</text>
            <dbReference type="Rhea" id="RHEA:51300"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:134020"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:51301"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:51302"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-L-phenylalanine + H2O = (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + L-phenylalanine</text>
            <dbReference type="Rhea" id="RHEA:64132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:77016"/>
            <dbReference type="ChEBI" id="CHEBI:149701"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:64133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:64108"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:59002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:64109"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:64110"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:64116"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:149697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:64117"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:64118"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-(9Z-octadecenoyl)-L-asparagine + H2O = L-asparagine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:64136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:149730"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:64137"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>N-(9Z-octadecenoyl)-L-glutamine + H2O = L-glutamine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:51356"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:134033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:51357"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N-(9Z-octadecenoyl)-L-leucine + H2O = L-leucine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:51360"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:134035"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:51361"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:51362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-(9Z-octadecenoyl)-L-lysine + H2O = L-lysine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:64192"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:149731"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:64193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N-(9Z-octadecenoyl)-L-methionine + H2O = (9Z)-octadecenoate + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:64144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:149732"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:64145"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N-(9Z-octadecenoyl)-L-serine + H2O = L-serine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:51352"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:134031"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:51353"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N-(9Z-octadecenoyl)-L-tryptophan + H2O = L-tryptophan + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:64176"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:149733"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:64177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-(9Z-octadecenoyl)-L-tyrosine + H2O = L-tyrosine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:64184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:149734"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-hexadecanoyl-L-phenylalanine + H2O = hexadecanoate + L-phenylalanine</text>
            <dbReference type="Rhea" id="RHEA:64124"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:149699"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:64125"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-octadecanoyl-L-phenylalanine + H2O = octadecanoate + L-phenylalanine</text>
            <dbReference type="Rhea" id="RHEA:64128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:149700"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:64129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>an N-acyl-L-amino acid + H2O = an L-alpha-amino acid + a carboxylate</text>
            <dbReference type="Rhea" id="RHEA:15565"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="EC" id="3.5.1.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:15566"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:15567"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>an N-acyl-aromatic L-alpha-amino acid + H2O = an aromatic L-alpha-amino acid + a carboxylate</text>
            <dbReference type="Rhea" id="RHEA:54184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:84824"/>
            <dbReference type="ChEBI" id="CHEBI:138093"/>
            <dbReference type="EC" id="3.5.1.114"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:54185"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="23">
            <dbReference type="Rhea" id="RHEA:54186"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918700.1">
        <property type="nucleotide sequence ID" value="XM_014063225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9H8"/>
    <dbReference type="GeneID" id="106546364"/>
    <dbReference type="KEGG" id="tsr:106546364"/>
    <dbReference type="CTD" id="148811"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004046">
        <property type="term" value="F:aminoacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990845">
        <property type="term" value="P:adaptive thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043605">
        <property type="term" value="P:amide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.900:FF:000003">
        <property type="entry name" value="N-fatty-acyl-amino acid synthase/hydrolase PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000027">
        <property type="entry name" value="N-fatty-acyl-amino acid synthase/hydrolase PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="32" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="29" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 28" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="30">
        <location>
            <begin position="261"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" evidence="27">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="27">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_013918700.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="58576" checksum="832C047236BA39E3" modified="2020-10-07" version="1">MATFGARLRGVLCGAGLGLCGIVSVLLIAMLLRAYGTRPSVPALGLRGKQQQGRQRRVEAAFSPRELQELKEVLQGAVRIQTVSFSPDDQNITALEEFEPYIYKVFPVVFSTSFIQHEVIGDYSHLFTIQGSNPQLQPYMLLAHIDVVPAYPDGWDVSDPFSAVEHDGFIYGRGTLDNKNSAMGILHSLEFLLRRNYRPQRSFYIGLGHDEEVGGKKGAMKIVAELESRGIQLSFVLDEGSFIFDGVIPKIEKPVAVIGITEKGSLNVNLSVTSQVGHSSAPPKRTSIGILSEALYKLETQPMPNLFGHGPEIMMFEQLVPVFDFPLNIIMANLWLFGPLVGWFLERTPGTNALVRTTTAITMFHAGIKTNVIPPKADATVNFRIHSSQTVEEILEILDKTINDKRVKIEVMDTFDPPPVSPWDDQTFAVRVFRQTILDVFPDVASVVPGICIGNTDSRHYNSLTKAIYRFNSVTFKADDLPRFHGLNERISVEDYAKQVEFFFQLIQNCDLNQPTEPHTNLHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9V2</accession>
    <name>A0A6I9Y9V2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylation factor-like protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ARL6</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919320.1">
        <property type="nucleotide sequence ID" value="XM_014063845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9V2"/>
    <dbReference type="GeneID" id="106546869"/>
    <dbReference type="KEGG" id="tsr:106546869"/>
    <dbReference type="CTD" id="84100"/>
    <dbReference type="OrthoDB" id="442317at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04157">
        <property type="entry name" value="Arl6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000457">
        <property type="entry name" value="ADP-ribosylation factor-like protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041839">
        <property type="entry name" value="Arl6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 16 18" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013919320.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="24130" checksum="60C5EB630FBD4DAA" modified="2020-10-07" version="1">MVLCGVKSRHCLCHSIQIMGLFDKLACWLGLKKKEVHVLCLGLDNSGKTTIINKLKPYNAQVQDIVPTIGFSIENFKTSRYLSFTVFDMSGQGRYRNLWEHYYGDCQAIIFVIDSSDILRMVVAKEELDTLLAHPDLKHRRIPILFFANKMDLRDAVSSVKVSHLLTLENIKDKPWQICASDALKGEGLQEGVDWLQDQITQSDPNNEDMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA82</accession>
    <name>A0A6I9YA82_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pumilio homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KIAA0020</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress. Binds to double-stranded RNA or DNA without sequence specificity. Involved in development of the eye and of primordial germ cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919970.1">
        <property type="nucleotide sequence ID" value="XM_014064495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA82"/>
    <dbReference type="GeneID" id="106547359"/>
    <dbReference type="KEGG" id="tsr:106547359"/>
    <dbReference type="CTD" id="9933"/>
    <dbReference type="OrthoDB" id="497380at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000207">
        <property type="entry name" value="Pumilio RNA-binding family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001092">
        <property type="entry name" value="Pumilio RNA-binding family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="13">
        <location>
            <begin position="121"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="11">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013919970.1"/>
        </source>
    </evidence>
    <sequence length="632" mass="71651" checksum="E0ED30F99581C0D7" modified="2020-10-07" version="1">MDTKRKFIGKGGKTGRGKKKLTKNNADPGHPKRVIRKNEDIKPTFKSKKNEKGGKKPNKTGVKHWKSKQLSGNVSRKRKFQGDTEGSQPKKPKWADIKKKKKEIKQSRQLNEKTNYDIIIKGKQIWEALRRKNCDKEKRKKLLSDLQKLLHGKIKDMAFAHDSTRVIQCYIQFGSEEQRQEVFEELKESFTELSKSKYSRNIVKKLLMYGTKPQIAEIIKSFKGEVKKMLRHAEASTVVEYAYNDKAILEQRNMLTEELYGNTFQVYKTADQSTLGKVLEIQPEKREAIMDEIKQILTPMAQKEAVIKHSLVHKVFLDFFTYAVPKQRSEMIEAIREAVIYLAHTHDGARVAMHCLWHGTPKDRKVIMKTMKTYVEKIATGEFSHLVLLAAFDCIDDTKLVKQLIISELNSNLAAVINNKYGRKVLLYLLSPRDPTYFSPAIIKILQQGDENSHSKKDADVRHGELLEAISTPLLQHLNAHAEEMVMDKAAFIVVTGILKAAVGDVQPAMKAIAGLAARKMIPGGEDGQLHIAEHPAGHLVLKWLIEQDEKMSQSGREGCFARILIDHVGIDLLKTWVDVNRGAIVLCRLLQSSDQEVATQVKDGLKSIIPKLKRTKDCTAAAKALLEKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAQ6</accession>
    <name>A0A6I9YAQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106548061</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <text evidence="10">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920840.1">
        <property type="nucleotide sequence ID" value="XM_014065365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAQ6"/>
    <dbReference type="GeneID" id="106548061"/>
    <dbReference type="KEGG" id="tsr:106548061"/>
    <dbReference type="OrthoDB" id="26399at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000105">
        <property type="entry name" value="Exportin for tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="11">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013920840.1"/>
        </source>
    </evidence>
    <sequence length="961" mass="110153" checksum="96B3037A16BEC709" modified="2020-10-07" version="1">MDEQALLGLNPNADLDFRQRALAYFEQLKISPDAWQLCAEALAQRIYSDDHIKFFCFQVLEHQIKYKYSQLTELQQQLIRETLVTWLQAQMMNSQSEKTFIRNKAAQVFALLFVTEYLTKWPKFFFDILSVVGLNPRGVDLYLRILMAVDAELVDRDVVHTSEEARRNTLLKDTMREQCIPSLVESWYQILQTYQYTNSELTCQCLEVVGAYVSWIDLSLIANERFINMLLGHMSIEVLREEACDCLFEIVNKGMDPIDKTKLVETLCQVLQSAGLFIVDQEEDVDFLARFSKLINGMGQALIASWSKLVKIGDLKNAQDSLQAIEAKVALMLQLLIHEDDDISSNIIGFCYDYLHILKQLPVLSEQQKANVEAIMLAVMKKLTYDEEYNFENEGEDEAMFVEYRKQLKLLLDRLAQVSPELLLVSVRRVFNTTLQNWQTKPFMEVEVAIRLLYMLAEALPVSHSIHFTGDTKAGALQDMMRTLVTSGISTYQHTSVTLEFFETVVRYEKFFSLEPQHIPNVLIAFLDHRGLRHTNPKVRSRTAYLFSRFVKSLNKQMNPFIEDVLNRIQDLLELSPPENGYQALLTSDDQLFIYETAGVLIVNSEYSTERKQSLMRNLLTPLMERFKVLLEKLVMSQDEDRQMALADCLNHAVGFASRTSKAFSNKQTVKQCGCSEVYLDCLQTFLPALSCPLQKEVLRSGVRTFLHRMIICLEEEVLPFIPSASEHMLKDCEAKDLQEFIPLINQITAKFKTQVSPFLQQMFMPLLQAIFEVLHQPAEDNDQSAALEKQMLRRSYFAFLQTVTGSGMSEVIANQGAENVERVLFTIIQGAVDYPDPIAQKTCFIILSKLVELWGGKDGPVGFADFVYKHIVPACFLAPLKQTFDLADAQTVLALSECAVTLKTIHLKRGLECIQYLQQKYLPSLQVAPEIIQEFCQALQQPDAKVFKNYLKVFFQRAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB58</accession>
    <name>A0A6I9YB58_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chloride transport protein 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106548494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921348.1">
        <property type="nucleotide sequence ID" value="XM_014065873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB58"/>
    <dbReference type="GeneID" id="106548494"/>
    <dbReference type="KEGG" id="tsr:106548494"/>
    <dbReference type="OrthoDB" id="428525at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015108">
        <property type="term" value="F:chloride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000057">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051280">
        <property type="entry name" value="Cl-channel/antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689:SF158">
        <property type="entry name" value="H(+)_CL(-) EXCHANGE TRANSPORTER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013921348.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="31609" checksum="12CCF0A63592CA73" modified="2020-10-07" version="1">MEFDSGEEVDEKVNEKCHLWTIVDLGFFILMGVGGGLLGATFNCINKRLAKYRMRNVHPKPKLVRVLESLLVCLTTTLVIFVASMVLGECRPLSSADHGDNSSSFSLQDSSSEEVNSSIKTFFCHNNTYNDMATLFFNPQESAILQLFHQEGTFSPVTLSLFFLLYFLLSCWTYGISVPSGLFVPSLLCGASYGRLVANLLESYIGLDHVYSGTFALIGAAAFLGGVVRMTISLTVILIESTNEITYGLPIMITLMVAKWTGDLFNKGIYDIYVALRGVPLLEWEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB59</accession>
    <name>A0A6I9YB59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase ZFP91</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase ZFP91</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Zinc finger protein 91 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ZFP91</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with MAP3K14/NIK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013921351.1">
        <property type="nucleotide sequence ID" value="XM_014065876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB59"/>
    <dbReference type="GeneID" id="106548497"/>
    <dbReference type="KEGG" id="tsr:106548497"/>
    <dbReference type="CTD" id="80829"/>
    <dbReference type="OrthoDB" id="7852576at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000183">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZFP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000356">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZFP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF11">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZFP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="348"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="436"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013921351.1"/>
        </source>
    </evidence>
    <sequence length="576" mass="64145" checksum="8242E054FC0330BF" modified="2020-10-07" version="1">MPGAPEQAETQEEEPEQQQQEREEERTPPLSGRRRPLPPPIVSAEQGAESSGSRVLRGCRERGRAAAAAAAASRRRKAEYPRRRRSSPGARQNQEPEELPPETGAPPSAAAPSGTTGRKGTPRSSCLDKATVKDPKEETEEKEISTTAAAGASVTTTKPSRGWRSNRAATTVQQHDVENSRSSRSKTGSLQLICKSEPNADQLEFEVTEEHSSPTRISDEEEMLISEEEVPFKDDPRDETYKPHLDRDAPKQRKKTGKGKEEKEKQKEIKVEVEVELKEESEFREDEDPPRKRGRRRKDDKSPRLPKRRKKPPIQYVRCEMEGCGTVLAHPRYLQHHIKYQHLLKKKYVCPHPSCGRLFRLQKQLLRHAKHHTDQRDYICEYCARAFKSSHNLAVHRMIHTGEKPLQCEICGFTCRQKASLNWHMKKHDADTFYQFSCNICGKKFEKKDSVVAHKAKSHPEVLIAEALAANAGALITSTDILGPDTESVVQPTDGQGLPLLPDPIGNTTPAECLLLNSEGMPKAYCSGGDRVNLMADGKIFVGSASSENTEGLVINTEILGATTEVLIEDSDSAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBX0</accession>
    <name>A0A6I9YBX0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Olfactory receptor 6M1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106548692</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921595.1">
        <property type="nucleotide sequence ID" value="XM_014066120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBX0"/>
    <dbReference type="GeneID" id="106548692"/>
    <dbReference type="KEGG" id="tsr:106548692"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15912">
        <property type="entry name" value="7tmA_OR6C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF155">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921595.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="35881" checksum="BBEA07FCB4AD18EF" modified="2020-10-07" version="1">MEVQNVTIVREIFLLGFIFEPNLEIILFMVFLLIYLATVLGNTLIIALICIDHRLQTPMYFFLSNLSAIEIFMTTSVLPKMLANLLSERKTISFAGCFTQSYFYFFMGSTEFILFAAMSFDRYMAICYPLRYSILVTGKVCVNIVIGSWIGGFFSVFLSTVLKARLTYCRHVINHFFCDSVPLLHLACQDITMIELVDFLVSLILLLGSLSFTATSYIYIISTIVKIPSAEGRKKAFCTCASHFTVVSMGYGISIFVYVRPSQSDSMSLNKALAIFSGILTPFLNPFIFSLRNEQMKEVLRDVLKNMSFKIKSICSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD54</accession>
    <name>A0A6I9YD54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="17">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="17">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">B4GALT4</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-acetyl-D-glucosamine + UDP-alpha-D-galactose = beta-D-galactosyl-(1-&gt;4)-N-acetyl-D-glucosamine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17745"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60152"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="2.4.1.90"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:17746"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Golgi apparatus membrane</location>
            <topology evidence="2 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013921989.1">
        <property type="nucleotide sequence ID" value="XM_014066514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD54"/>
    <dbReference type="GeneID" id="106549008"/>
    <dbReference type="KEGG" id="tsr:106549008"/>
    <dbReference type="CTD" id="8702"/>
    <dbReference type="OrthoDB" id="10016069at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003945">
        <property type="term" value="F:N-acetyllactosamine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000028">
        <property type="entry name" value="beta-1,4-galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF9">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14 17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 17" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="19">
        <location>
            <begin position="83"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="18">
        <location>
            <begin position="219"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013921989.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="40692" checksum="2930613CBA8F1BD8" modified="2020-10-07" version="1">MGFGSASFHLSYKLKLLMLITLCVLLIGWATSTYLVDILHETPAAKGMIESFMKPTTIKEEQMENSKRKAMLIEKLPTAIPACPFLSPYLRGPSKLIFRASLTLEELQKENPSVAKGRHRPKDCTAQQRVAILIPHRNREKHLLYLLENLHPFLQRQQLDYGLYVIHQAGNAKFNRAKLLNVGYLEALKEENWDCFIFHDVDLVPENDFNIYVCGSQPKHLVVGRNSTGYRLRYQGYFGGVTALTREQFLKVNGFSNNYWGWGGEDDDLRIRVEMQKMKIIRPSPSIAKYTMIFHTRDQGNEANGQRMNLLRQVSRVWKTDGLNSCSYKLLSKEYNLLYTNITVDFGRQTKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEE3</accession>
    <name>A0A6I9YEE3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein KIAA0195 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KIAA0195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922375.1">
        <property type="nucleotide sequence ID" value="XM_014066900.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549300"/>
    <dbReference type="KEGG" id="tsr:106549300"/>
    <dbReference type="CTD" id="9772"/>
    <dbReference type="OrthoDB" id="5568754at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039720">
        <property type="entry name" value="TMEM94"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13219">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 94"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13219:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 94"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1139"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1170"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1213"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1278"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1317"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1356"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="932"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013922375.1"/>
        </source>
    </evidence>
    <sequence length="1406" mass="158708" checksum="9D4EE0DA5E8D9652" modified="2020-10-07" version="1">MTIPFFTCSFLWLRDFHFHIPKKRTRQSQEREQQQIVWLAGQSQKFYKQDEMTTNLGLTTTKALTILRDQLSTLLEGHQKERKKVISWKEVWKASFLYHGNRCSCFHWPGASLMLLTMLLLFCCYGSQPQGSEGSEIVNALALFFLVLLDLFVIGRQERMKRREIERRLRKIISRIDDALKDSKELIWTKTMYPDLHMPFAPSWSLHWVYRDGHLVNLPVSLLVEGDIVALRPGQESFTSLRGIKDDEHIVLEPGDLFPPFSPPPSPNGEVKKGPQNPQMFRLFRVTKTPVIDNVRLCLDMGLSRRVTALDNERFTVQSVMLKFAVPVVLLGFLITNAMRFFFMVGGTVPWQYMFLQLQVNGVLPILPLLFPVLWILATAFGEARVLAQMSKASPTSLLAKFSEDTLSSYTEVVSSQEMLRCIWSHFLQVLKGKSPTLTFTSSLLHSLGSVTVLCCVDKQGILSWPNPSPETVLFFSGKVEPPHSSHEDLTDELSTRSFCHPEMDEEPHERDALLSGPLLDVIRISNEQDRAEWSTSNTLKSSDTQIHKKPACRSKHPSGSNVSFSKDIEGGEEETIQVESDIDGLACEAEDFVCDYHLEMLSLSQDQQNPSCIQFDDSNWQMHLTSLKPLGLNVLLNLCNASVTEPLCRFSDHLCNLALQETHHAVLPVHIPWGLCELARLIGFTPGAKELFKQENYMALYRLPSDEMVKEVMLGKLSFITKRRPPLSHMISLFVRDTTTSTEQMLSHGTADIILEACTDFWDGSDIYPLSGSDRKKVLDFYQRACLSGYCSAFSYKPMHCALSSLLNGKCIELVQVPGQNAIFTSCDLPGTIPIKQSSRRNSWSSDEGIGEVMEKEDCIQALSGQIFMGMVSSQYQARSDVVRLIEGLVNACIRFVYFSLEDELKSKVFAEKMGLETGWNCHISLTPNGDVPGSEIPPSSPSHAGSLHEDLPQVSREDAEGILLMEEEGHSDLISFQPTDSDIPSFLEDCNRAKLPRGIHQVRPHLQNIDNVPLLVPLFTDSTPETMCEMIKIMQEYGEVTCCLGSSANLRNNCLFLQSDISIALDPLYPSRCSWETFGYATSTNLTHISDELTPLQLSGQLNSLSCSLSICPEETIGIIRLIEQARHATYGIRKCFLFLLQCQLTLVIIQFLSCLAQLPPILSTTDILWLSCFCYPLLSVSLLGKPPHSSIMSMATGKNLIFIPKKTQHYFLFCFLLKFSLTICSCLACFGYLLQDYCANFNSSSFSVNVTGCFSLVLQSGAERPPDWFGTFSNVLLLAQKFTAGMIVLHIVFISVTHVHRTKPLWKKSPFTNFWWTLTVVVVLLGQIVQTFLDLKLWVNLESPVTYQMNDIPMASWLLGLLSLVLVVIINETVKLHEIRVRVRYQKRQKLQFETKLGMNSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEH1</accession>
    <name>A0A6I9YEH1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thymosin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMSB15B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
            <location evidence="1 5">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the thymosin beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922573.1">
        <property type="nucleotide sequence ID" value="XM_014067098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEH1"/>
    <dbReference type="GeneID" id="106549444"/>
    <dbReference type="KEGG" id="tsr:106549444"/>
    <dbReference type="CTD" id="286527"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.520:FF:000001">
        <property type="entry name" value="Thymosin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.520">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001152">
        <property type="entry name" value="Beta-thymosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038386">
        <property type="entry name" value="Beta-thymosin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12021">
        <property type="entry name" value="THYMOSIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12021:SF11">
        <property type="entry name" value="THYMOSIN BETA-15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01290">
        <property type="entry name" value="Thymosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001828">
        <property type="entry name" value="Thymosin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00152">
        <property type="entry name" value="THY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00500">
        <property type="entry name" value="THYMOSIN_B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013922573.1"/>
        </source>
    </evidence>
    <sequence length="45" mass="5215" checksum="653D6982485590CB" modified="2020-10-07" version="1">MCDKPDLSEVEKFDKKKLKKTNTEEKNTLPSKETIEQEKETAKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEX7</accession>
    <name>A0A6I9YEX7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UBL7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Interferon-stimulated protein that positively regulates RNA virus-triggered innate immune signaling. Mechanistically, promotes 'Lys-27'-linked polyubiquitination of MAVS through TRIM21 leading to enhanced the IFN signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds ubiquitin. Interacts with MAVS; this interaction enhances TRIM21-dependent 'Lys-27'-linked polyubiquitination of MAVS.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922540.1">
        <property type="nucleotide sequence ID" value="XM_014067065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEX7"/>
    <dbReference type="GeneID" id="106549414"/>
    <dbReference type="KEGG" id="tsr:106549414"/>
    <dbReference type="CTD" id="84993"/>
    <dbReference type="OrthoDB" id="10016665at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14326">
        <property type="entry name" value="UBA_UBL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01815">
        <property type="entry name" value="Ubl_UBL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000192">
        <property type="entry name" value="Ubiquitin-like 7b (bone marrow stromal cell-derived)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000139">
        <property type="entry name" value="ubiquitin-like protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047878">
        <property type="entry name" value="UBL7_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047877">
        <property type="entry name" value="UBL7_Ubl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF25">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="39"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="334"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="271"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013922540.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="40616" checksum="65C81BFD08BC4BC1" modified="2020-10-07" version="1">MSLSECYIAVKLADQPLAPKSILRLPESELGECPLGGCSISYLKQLITGKLQESIPDPEFIDLIYCGRKLRDEQTLEFYGIQSGSTIHVLRKSWPEPEQKPEPVDKMAAVREFRVLHTALHSSPVFRDSVFKMLGNKESLDQIIVATPGLSSDPIALGVLQDKDLFSVFADPAMLDTLIPSHPALVNAIVLVLHSVAGSAPLPTAETSSRSSMPSSSYRDMPGGFLFEGLSDDEDDFHQSTRTTPSSSTSGSRPASLGYTGAAGPRPITQSELATALALASTPESSSHTPTPGTQGHSSGTSPMSSSVQSGTPITNDLFSQALQHALQASGQPNLQSQWQPQLQQLRDMGIHDDELSLRALQATGGDIQAALELIFAGGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF36</accession>
    <name>A0A6I9YF36_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">LOW QUALITY PROTEIN: segment polarity protein dishevelled homolog DVL-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DVL3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DSH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922782.1">
        <property type="nucleotide sequence ID" value="XM_014067307.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549609"/>
    <dbReference type="KEGG" id="tsr:106549609"/>
    <dbReference type="CTD" id="1857"/>
    <dbReference type="OrthoDB" id="10031689at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04438">
        <property type="entry name" value="DEP_dishevelled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06717">
        <property type="entry name" value="PDZ_Dishevelled-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.130:FF:000009">
        <property type="entry name" value="Dishevelled segment polarity protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000040">
        <property type="entry name" value="segment polarity protein dishevelled homolog DVL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000014">
        <property type="entry name" value="Segment polarity protein dishevelled homolog DVL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024580">
        <property type="entry name" value="Dishevelled_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008339">
        <property type="entry name" value="Dishevelled_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003351">
        <property type="entry name" value="Dishevelled_protein_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001158">
        <property type="entry name" value="DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038207">
        <property type="entry name" value="DIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015506">
        <property type="entry name" value="Dsh/Dvl-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10878">
        <property type="entry name" value="SEGMENT POLARITY PROTEIN DISHEVELLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10878:SF6">
        <property type="entry name" value="SEGMENT POLARITY PROTEIN DISHEVELLED HOMOLOG DVL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02377">
        <property type="entry name" value="Dishevelled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00778">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12316">
        <property type="entry name" value="Dsh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01760">
        <property type="entry name" value="DISHEVELLED"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01761">
        <property type="entry name" value="DISHEVELLED1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50841">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="DIX" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="200"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="9">
        <location>
            <begin position="314"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="36"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="457"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="495"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="541"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013922782.1"/>
        </source>
    </evidence>
    <sequence length="603" mass="65720" checksum="06B368EA15552EBA" modified="2020-10-07" version="1">MDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHSDAGSVCAESQPELPPSIERTGGIGDSRPPSFHPNSGGSRENLDNETETDSVVSVQRERPRRKEGPEHAPRVNGMTKGDRRRELGGYESSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVARIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDTNFENMSNDDAVRVLREIVHKPGXDLLLTGLENIGICLDDFHLSIHSDMATIVKAMASPESGLEVRDRMWLKITIPSAFIGSDVVDWLYHHVEGFTDRRESRKYASNLLKAGYIRHTVNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPLAFPYQYPPPHPYNPHPGFPEPGYSYGGGSAGSQHSEGSRSSGSNRSGSERRKDRDPKAGESKSGGSGSESDHTSRSSIRRERAASECSVPGSQRSHHSMAHSVRSHHSQQSYGPPGLPPLYSPPMLLMPPPPSALGPPGAPPGRDLASVPPELTASRQSFRMAMGNPTKNYGVFDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFD6</accession>
    <name>A0A6I9YFD6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Filamin-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106549701</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922882.1">
        <property type="nucleotide sequence ID" value="XM_014067407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFD6"/>
    <dbReference type="GeneID" id="106549701"/>
    <dbReference type="KEGG" id="tsr:106549701"/>
    <dbReference type="OrthoDB" id="5334309at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21308">
        <property type="entry name" value="CH_FLNA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000006">
        <property type="entry name" value="Filamin-B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000008">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000001">
        <property type="entry name" value="Filamin-C isoform b"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000122">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF5">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="53"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="176"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="286"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="386"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="485"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="590"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="639"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="742"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="841"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013922882.1"/>
        </source>
    </evidence>
    <sequence length="959" mass="103156" checksum="0472537618E1941F" modified="2020-10-07" version="1">MSGSQARLHQAAGAGAVSGGGGGGGGAGGAQGEVSAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLGDGLRLIALLEVLSQKKMGRKYNARPTFRQMQLENVSVALEFLERESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEDDEEVKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVNNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPMGNMVKKKAEFTVETISAGLGEVLVYVEDPAGHREEAKVIANNDKNRTFSVWYVPKVTGVHKVTVLFAGQHIAKSPFEVNVDKSHGDASKVTAQGPGLEPTGNIANKTTYFEIFTAGAGVGELEVIIQDPTGKKGTVEQQLEDKGNSTYRCSYKPTLEGTYTIYITFGGIPIPRSPYTVTVGQACNPSACRAVGRGLQPKGVRVKETADFKVYTKGAGSGELKVTIKGPSFSVEGPSQAKIECDDKGDGSCDVRYWPQEPGEYAVHVLCNNEDIKLSPFMAEIKAAPKDFYPEKVKAHGPGLEKTGVAINKPAEFTVDARSGGKAPLKVQVQVNIGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPAEADIDFDIIRNDNDTFTVKYTPRGAGSYTIMVLFADQATPTSPIRIKVEPSHDASKVKAEGPGLNRTGVEMGKPTHFTVNTKPAGKAKLDVQFTGPAKGDAVRDSEVIDHHDNTYTVRYTPVQQGNLGVNVTYGGDHIPKSPFNVGITPTLDLSKIKISGLGDKVEVGKDQEFTVKSKGAGGQGKVAAKIVGPSSKPVPCKVEPGLSSDNSVVKFIPREEGPYEVDVTYDGVPVPGSPFPVEAVPPTNPSKVLHNTDYKYFVSLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFX5</accession>
    <name>A0A6I9YFX5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA repair nuclease/redox regulator APEX1</fullName>
            <shortName evidence="15">APEN</shortName>
            <shortName evidence="15">REF-1</shortName>
            <ecNumber evidence="15">3.1.11.2</ecNumber>
            <ecNumber evidence="15">3.1.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">APEX nuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Apurinic-apyrimidinic endonuclease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Redox factor-1</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="15">DNA repair nuclease/redox regulator APEX1, mitochondrial</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="19 20 21 22" type="primary">APEX1</name>
    </gene>
    <organism evidence="18 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20 21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13 15">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="15"/>
    <dbReference type="EC" id="3.1.21.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013923165.1">
        <property type="nucleotide sequence ID" value="XM_014067690.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923166.1">
        <property type="nucleotide sequence ID" value="XM_014067691.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923167.1">
        <property type="nucleotide sequence ID" value="XM_014067692.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923168.1">
        <property type="nucleotide sequence ID" value="XM_014067693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFX5"/>
    <dbReference type="GeneID" id="106549931"/>
    <dbReference type="KEGG" id="tsr:106549931"/>
    <dbReference type="CTD" id="328"/>
    <dbReference type="OrthoDB" id="498125at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09087">
        <property type="entry name" value="Ape1-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000009">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19 20 21" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="17">
        <location>
            <begin position="65"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="12">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="14">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="14">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="14">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013923165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013923166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013923167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013923168.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35968" checksum="20F507A0C4C5C7DF" modified="2020-10-07" version="1">MPKRVKKEEEEILPAEEGTTEPEPKKAMGDAPKPEKEADGPILYQDPPDKLTTPSDKKYTMKITSWNVDGIRAWFKKKGLEWVTEEDPDVLCLQETKCTEKQLPAEIRDLAEYPHKFWACSTDKEGYSGVAMLSKNKPLDVTYGIGEEEHDKEGRVITAEFPSYFLVTAYVPNAGRGLVRLEYRQRWDEAFRSYLEGLVARKPLILCGDLNVAHQEIDLKNPKGNKKNAGFTPEERAGFTQLLEAGFVDTFRHLYPDTPNAYTFWTYMMNARAKNVGWRLDYFVISKELLEGLCDSKIRSSALGSDHCPITLYVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG56</accession>
    <name>A0A6I9YG56_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Upstream stimulatory factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106550002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923253.1">
        <property type="nucleotide sequence ID" value="XM_014067778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG56"/>
    <dbReference type="GeneID" id="106550002"/>
    <dbReference type="KEGG" id="tsr:106550002"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF2">
        <property type="entry name" value="UPSTREAM STIMULATORY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="72"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="124"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013923253.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="23732" checksum="9BBF0D22B002DE83" modified="2020-10-07" version="1">MTDLPLQNGNPHFFLVRQCRRKNCDVQEGSGADSPISPTTLARIRGKEVLCTRVTTSLCLSSRQVIVIGSHMEVFRWENVERRRRDKINNWIVQLSKIIPDCNTDNTKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNDLLRQQVAQPKFSALSRFRFEMVASHKAKYRQSSTYTATTEATVSVVKGDIRLQLRPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIG4</accession>
    <name>A0A6I9YIG4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ankyrin repeat and SOCS box protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ASB13</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924163.1">
        <property type="nucleotide sequence ID" value="XM_014068688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIG4"/>
    <dbReference type="GeneID" id="106550732"/>
    <dbReference type="KEGG" id="tsr:106550732"/>
    <dbReference type="CTD" id="79754"/>
    <dbReference type="OrthoDB" id="10252328at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF53">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX CONTAINING 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="129"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="6">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013924163.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="24499" checksum="8D71AA2EBD75665A" modified="2020-10-07" version="1">MTRTTSPSLRGQTRCVEILLAAGAQVDARNIDGSTPLCDACASGSIECVKVLLSHGAKVNPPLYIASPLHEACLNGSAECVRLLIEVGANLEAHDCHFGTPLHVACAREQLDCVKLLLNAGANVNAAKLHETALHHAAKVRNADLAEMLIEFGGNVYAQDNRGKKPSDYSQRNSCTAKCLAYYEGNPLRLTQLCRLSLRRALGQKALEKIPHLPIPTKFIAFLSYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIX4</accession>
    <name>A0A6I9YIX4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ankyrin repeat domain-containing protein 27-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106550734</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924166.1">
        <property type="nucleotide sequence ID" value="XM_014068691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIX4"/>
    <dbReference type="GeneID" id="106550734"/>
    <dbReference type="KEGG" id="tsr:106550734"/>
    <dbReference type="OrthoDB" id="9048484at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031436">
        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924166.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="26696" checksum="C26F96638C5C27D4" modified="2020-10-07" version="1">MYACVNGYQEIAAILLQHGASVNLPNNQGNTPLHKAVIGNYEAVVQVLLQNGALVHLRNNKQCTPLDYAQPNSGLLKLLQAAASEEDDGQMLCKTHMTRMIRKKISFKTPETTDDLIAQRLQLLESIHRFNKSRNLRKAITKDKSVPNFDEELLHQLAIKNFNNKQNPVDVLEKIQTHNLDANSCDGPVTAEGDAEVTPLRKREVHRSHTISVALSTADSSDFYSLPRDASAASTDSCPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ21</accession>
    <name>A0A6I9YJ21_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 14B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LRRC14B</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRAME family. LRRC14 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923785.1">
        <property type="nucleotide sequence ID" value="XM_014068310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ21"/>
    <dbReference type="GeneID" id="106550406"/>
    <dbReference type="KEGG" id="tsr:106550406"/>
    <dbReference type="CTD" id="389257"/>
    <dbReference type="OrthoDB" id="8875973at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000313">
        <property type="entry name" value="Leucine rich repeat containing 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050694">
        <property type="entry name" value="LRRC14/PRAME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224:SF27">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224">
        <property type="entry name" value="SIMILAR TO PREFERENTIALLY EXPRESSED ANTIGEN IN MELANOMA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013923785.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="58539" checksum="5A6F56B7B99A54FE" modified="2020-10-07" version="1">MHSLRFLSAEVLVSKGQLVRQNLSHLAHNLFPLLFKASYLQEQVEVIHDLVENWPLAEFNIGKLLGKTADHPEDISNRACSLCLASCLAGLKDYVLNCSSPYAKRLKTVDLTGIKDVEVQLCPCRKTMGRWARTELLSRTCYDLLVDMQNLPFLPDVFEVSVDVFADIFVTERSYEVVVQALLMRCHCPLKIRCKAFRVDNLALRKLFYIIKLTEPSSLGKFEVVHNVRLHMEHLQVLFNNVQFPKLASLTLPAGTFDVRRFTPEDEATLSGIGEKMSQMTQLTELSLAFSILTGRLRKLLSPLKTPLKMLDVSNCSLNHLDMAYLANSLHSEKLEALDISGHDVADLYPSTFFKLLNHSSYTLKSLTLEECNIQDIHINMLILGLVPCRKLEELKFLGNPLSSRALKCLFNIFIDFPRLKYIEFPVPRDCYANNISYPIGESDLSKFDHQKYERIVEDLHMILLQAKREDIKASTPLYGGYDAAIQETGNELGISLLKSFQDALQSFSVALKEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJF5</accession>
    <name>A0A6I9YJF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein FAM78A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FAM78A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924377.1">
        <property type="nucleotide sequence ID" value="XM_014068902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJF5"/>
    <dbReference type="GeneID" id="106550912"/>
    <dbReference type="KEGG" id="tsr:106550912"/>
    <dbReference type="CTD" id="286336"/>
    <dbReference type="OrthoDB" id="9971204at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029638">
        <property type="entry name" value="FAM78"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31655">
        <property type="entry name" value="PROTEIN FAM78A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31655:SF3">
        <property type="entry name" value="PROTEIN FAM78A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924377.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="32526" checksum="F50EB8492C2994C2" modified="2020-10-07" version="1">MDISFELTDEEDCWCLLAIVLVLWAMGCIQSISCKAKGFRESITVLEIKTSIDPVPTNIDESSSVVLRYRTPHFRASAQVLLPPVPKKETWIVGWIQACSHMEFYNYYGDHGMSSWELPELLEGKIQAISDSDGVNYPWYGNTTETCTIVGPTKKEAKFTISMNDNFYPSVTWAVPVSDSNVPKLTCIHRDQSFTTWLVATNTTTNEMVILQTIKWRMKLGIDVEPSHPLGRRAKLREPSAQEQPQVLSKNEPIPPSALVKPNANDAQVLMWRPKDGRPLVVIPPKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJM0</accession>
    <name>A0A6I9YJM0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Deoxycytidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DCK</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2'-deoxyadenosine + ATP = dAMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17256"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58245"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.76"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2'-deoxycytidine + a ribonucleoside 5'-triphosphate = dCMP + a ribonucleoside 5'-diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="2.7.1.74"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyguanosine + ATP = dGMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19201"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57673"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.113"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCK/DGK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924442.1">
        <property type="nucleotide sequence ID" value="XM_014068967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJM0"/>
    <dbReference type="GeneID" id="106550977"/>
    <dbReference type="KEGG" id="tsr:106550977"/>
    <dbReference type="CTD" id="1633"/>
    <dbReference type="OrthoDB" id="567086at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004136">
        <property type="term" value="F:deoxyadenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004137">
        <property type="term" value="F:deoxycytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004138">
        <property type="term" value="F:deoxyguanosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000461">
        <property type="entry name" value="Deoxycytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF19">
        <property type="entry name" value="DEOXYCYTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="15">
        <location>
            <begin position="15"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="19"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
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        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="88"/>
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        <ligand>
            <name>substrate</name>
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            <position position="119"/>
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        </ligand>
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        <location>
            <position position="124"/>
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            <name>substrate</name>
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    </feature>
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        <location>
            <begin position="179"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="14">
        <location>
            <begin position="231"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007420"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047656"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013924442.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="30019" checksum="E6A5785ABADFE144" modified="2020-10-07" version="1">MATPPKRSRSGVKKIAIEGNIAAGKSTFVNILKKAREEWEIVPEPVEKWCNVQDCRDDCEELTASQKSGGNLLQMMYEKPERWSFTFQAYACLSRIRAQLKSLEGKLKEPENAVVFFERSVYSDRYIFASNLYESDCMNETEWTIYQDWHSWMNQQYGSSLELDGIIYLRATPEKCLDRIYIRGRDEEQGIPMEYLEKLHYKHESWLQHRTLRTEFEYLQKVPVLTLDVNEDFKGNRDKHENMIEKVQEFLSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJX3</accession>
    <name>A0A6I9YJX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">MAM domain-containing glycosylphosphatidylinositol anchor protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MDGA2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013924461.1">
        <property type="nucleotide sequence ID" value="XM_014068986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJX3"/>
    <dbReference type="GeneID" id="106550993"/>
    <dbReference type="KEGG" id="tsr:106550993"/>
    <dbReference type="CTD" id="161357"/>
    <dbReference type="OrthoDB" id="6107927at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008046">
        <property type="term" value="F:axon guidance receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000240">
        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000262">
        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000303">
        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 1"/>
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    </dbReference>
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        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000165">
        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000243">
        <property type="entry name" value="MAM domain-containing glycosylphosphatidylinositol anchor protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050958">
        <property type="entry name" value="Cell_Adh-Cytoskel_Orgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080">
        <property type="entry name" value="CONTACTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080:SF35">
        <property type="entry name" value="MAM DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027039037" evidence="11">
        <location>
            <begin position="21"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="27"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="134"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="242"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="340"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="442"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="540"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="12">
        <location>
            <begin position="746"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013924461.1"/>
        </source>
    </evidence>
    <sequence length="956" mass="107780" checksum="BEFBE3D7AB5418FD" modified="2020-10-07" version="1">MDLAYGVVWLLTVLLEGISGQGVYAPPTVRIIHSGLACNIEEERYSERVYTIREGETLELTCLVTGHPRPQIRWTKTAGSASDRFQDSSVFNETLRITNIQRHQGGRYYCKAENGLGSPAIKSIRVDVYYLDDPIVTVHQSIGEAKEQFYYERTVFLRCVANSNPPVRYSWRRGQEVLLQGSDKGVEIYEPFFTQGETKILKLKNLRPQDYANYSCIASVRNVCNISDKMVSFRLSNKTASPSIKLLVDDPIVVNPGEAITLVCVTTGGEPLPTLTWVRSVGTLPEKIILKGGTLTIPAINSDDAGTYSCIANNNVGNPAKKSTNIIVRALKKGRFWITPDPYHKDDNIQIGREVKISCQVEAIPSEELMFSWFKNGRPLRSSERMVITQTDPDVSPGTTNLDIIDLKFTDFGTYTCVAALKGGGISDISIDVNISSSTVPPNLTVPQEKSPLVTREGDTIELQCQVTGKPKPIILWSRADKEVPMPDGTMQMESYDGILRIVNVSREMTGTYRCQTSQYNGFNVKPREALVQLIVQYPPAVEPTFLEIRQGQGRSVTMSCRVLRAYPTRVLTFEWRLGSKLLRTGRFDAQDSTEYIIRSLSRDSYGNYSCNIINEAGAGRCSFLVTGKAYAPEFYYDTYNPLWQNRARVYAYSLEWTQMNPDAVDRILTYHLGIRQAGQQRWWEQEITVNGKIQKGELITYNLTELIKPEAYEVRLTPVTIFGDGDATIRVIKYSAPMNPHLREFHCGFEDGNICLFTQDEKDSFDWTRQNIVLRDTKYTPNTGPNADRSGSKEGFFMYIEASSPRKEGDRARLTSPIFSIPPKNPYGTMNTAYCFSFYYNMYGQHIGTLNVYLRLKSQTSSESPFWSTSGNKGQHWFQARVNIHPNSSFQIVFEGIRGRGIEGDIAIDDISIVEGECTKSDYVNNHIPSEAVRTLPHIRSFPVFLLVSVLIHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKU0</accession>
    <name>A0A6I9YKU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="25">PARP</shortName>
            <ecNumber evidence="25">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">TNKS</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="25"/>
    <dbReference type="RefSeq" id="XP_013924988.1">
        <property type="nucleotide sequence ID" value="XM_014069513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKU0"/>
    <dbReference type="GeneID" id="106551406"/>
    <dbReference type="KEGG" id="tsr:106551406"/>
    <dbReference type="CTD" id="8658"/>
    <dbReference type="OrthoDB" id="4772757at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09524">
        <property type="entry name" value="SAM_tankyrase1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01438">
        <property type="entry name" value="tankyrase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000012">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000009">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000010">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000011">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000024">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase tankyrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF143">
        <property type="entry name" value="INVERSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="19 24" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 25" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 25" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0779">Telomere</keyword>
    <keyword evidence="8 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="297"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="330"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="417"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="453"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="486"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="579"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="612"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="645"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="732"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="765"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="798"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="27">
        <location>
            <begin position="926"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="28">
        <location>
            <begin position="1008"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="26">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013924988.1"/>
        </source>
    </evidence>
    <sequence length="1223" mass="132001" checksum="BE774CC6A2EA23D4" modified="2020-10-07" version="1">MAAASSSSRRSQQHHHHHPAHPPSHHSVQAPVSPPRSPNLSPTGVSSQQRPTLAGVEGEAPANSGREGTAGTERPFSPESACESSGPPPPPGSGASSAATTTSSSSSSGSSSSSSAASSPAAPDSPDPPPPPLQPLPPPVGSGAFRELLEACRNGDVTRVRRLVDATNVNAKDMAGRKSTPLHFAAGELLLFLYSVLLQHGADPNIRNTDGKSALDLADPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELKERLTYEFKGHSLLQAAREADLAKVKKTLALEVINFKQPQSHETALHCAVSSLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTPLHRAALAGHLQTCRLLLNYGSDPSIISLQGFTAAQMGNEAVQQILNENTPVHTSDIDYRLLEASKAGDLETVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASIISPASTPSCLSAASSIDNLAGPLAEIAVAGPSNAGDGATGTERKEGEVVGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFHCVSQGTILIDLAPDDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIVKPDTPSQTAVAAEQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLJ6</accession>
    <name>A0A6I9YLJ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fat storage-inducing transmembrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FITM2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925107.1">
        <property type="nucleotide sequence ID" value="XM_014069632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLJ6"/>
    <dbReference type="GeneID" id="106551511"/>
    <dbReference type="KEGG" id="tsr:106551511"/>
    <dbReference type="CTD" id="128486"/>
    <dbReference type="OrthoDB" id="5579088at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03230">
        <property type="entry name" value="FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046401">
        <property type="entry name" value="FITM1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF1">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013925107.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="30158" checksum="F00D7D01C4978395" modified="2020-10-07" version="1">MESVDRATRVLLPYLTHCSVRRMMPWALMSFMVIGSLVKELMPPAATYLSNKRNVLNVYFVKFAWAWTFCLLFPFISLTNYNVLQNVLPVLVRLFSLMVGTIIWYTCTSTFLLIQDFTGSCYKSSTLAVVTQEHSNQVQCLQAGGIWESFDISGHSFLLSYCILMILEEMAVMPSVKTFRGSRLHDVVNSLFLALACLTFIWLFMLLTTAVYFHDFWDKIFGILVGLSAWYGTYRFWYKSPLSPGFPPQRTLYFPKPNRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLN9</accession>
    <name>A0A6I9YLN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106551180</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Lipid-anchor</topology>
            <orientation evidence="2 13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924695.1">
        <property type="nucleotide sequence ID" value="XM_014069220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLN9"/>
    <dbReference type="GeneID" id="106551180"/>
    <dbReference type="KEGG" id="tsr:106551180"/>
    <dbReference type="OrthoDB" id="5971574at2759"/>
    <dbReference type="UniPathway" id="UPA00163"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000004">
        <property type="entry name" value="Phosphorylase b kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF4">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT ALPHA, SKELETAL MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11 13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 13" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013924695.1"/>
        </source>
    </evidence>
    <sequence length="448" mass="50894" checksum="544BA2E174605D02" modified="2020-10-07" version="1">MRGLLQCMMRQVDKVEDFKYSQSPKDCLHAKYNTSTCATVVGDDQWGHLQLDATSIYLLMLAQMTASGLHIIHNLDEVSFVQNLVFYIETAYKTADFGIWERGDKTNQGITELNASSVGMAKAALEALDEFDLFGTEGSPQSVIHVLPDEVQYCQSILHSMLPRASTSKEIDASLLSVISYPAFAVEDRDVVEKTKEEIIAKLQGRYGCCRFLRDGYRTPKEDPSRLYYEPAELKLFENIECEWPLFWTYLIIDGLFSGNVEQVQEYREALEGVLIKGKDGLRLVPELYCVPLEKVEEECRHPHTVDRLPVGKLPLMWAQSLYILGCLMAEGFLAPGEIDPLNRRFSTIPKPVVVVQVCLLAETEAIQAILREEGILVETVAEVHPMRIQPARILSYIYARLGRNKRMGLSGRPLRHMGVLATSKFYDIRDNIFAFTPEFIDQQQFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMI0</accession>
    <name>A0A6I9YMI0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA-binding motif protein, X chromosome isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RBMX</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925598.1">
        <property type="nucleotide sequence ID" value="XM_014070123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMI0"/>
    <dbReference type="GeneID" id="106551959"/>
    <dbReference type="KEGG" id="tsr:106551959"/>
    <dbReference type="CTD" id="27316"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12382">
        <property type="entry name" value="RRM_RBMX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000119">
        <property type="entry name" value="RNA-binding motif protein, X chromosome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012604">
        <property type="entry name" value="RBM1CTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08081">
        <property type="entry name" value="RBM1CTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925598.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="41540" checksum="5ABB5E0B8D1AEC67" modified="2020-10-07" version="1">MVEADRPGKLFVGGLNIETNEKALESVFGKYGRIVEVLLMKDRETNKSRGFAFVTFESPADAKDAARDMNGKSLDGKSIKVEQATKPTFETGRRGPLPRSRGPPRGLRGGRGSSGTRGPPSRGSHLGSSRGPLPMKRGPPPRSGGPPPKRPAPSGPVRSSSGMGGRAPLSRGRDSYGGPPRRDPLPSRRDVYLSPRDDGYSTKDSYSSRDYPSSRDTRDYAPPPRDYAYRDYGHSSSRDEYPSRGYSYSSYSDRDGYGGRDRDYSDHPSGGSYRDSYESYGNSRSAPPARGPPPSYGGSSRYDDYGSTRDGYGGSRESYSSSRSEVYSSGRDHVGRQDRGLPPSMERGYPPPRDSYSSSSRGAPRGGGRGGSRSDRGGGRSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YML7</accession>
    <name>A0A6I9YML7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Death domain-associated protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Daxx</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">DAXX</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DAXX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925045.1">
        <property type="nucleotide sequence ID" value="XM_014069570.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551464"/>
    <dbReference type="KEGG" id="tsr:106551464"/>
    <dbReference type="CTD" id="1616"/>
    <dbReference type="OrthoDB" id="7492809at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050681">
        <property type="term" value="F:nuclear androgen receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13151">
        <property type="entry name" value="DAXX_helical_bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13150">
        <property type="entry name" value="DAXX_histone_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.810:FF:000001">
        <property type="entry name" value="Death domain-associated protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2170:FF:000001">
        <property type="entry name" value="Death domain-associated protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.810">
        <property type="entry name" value="Daxx helical bundle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046378">
        <property type="entry name" value="DAXX_histone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046426">
        <property type="entry name" value="DAXX_histone-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031333">
        <property type="entry name" value="Daxx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038298">
        <property type="entry name" value="Daxx_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12766:SF7">
        <property type="entry name" value="DEATH DOMAIN-ASSOCIATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12766">
        <property type="entry name" value="DEATH DOMAIN-ASSOCIATED PROTEIN 6 DAXX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03344">
        <property type="entry name" value="Daxx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20920">
        <property type="entry name" value="DAXX_hist_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="21" id="KW-0137">Centromere</keyword>
    <keyword evidence="19" id="KW-0143">Chaperone</keyword>
    <keyword evidence="15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="17 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0678">Repressor</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Daxx N-terminal Rassf1C-interacting" evidence="25">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Daxx histone-binding" evidence="26">
        <location>
            <begin position="235"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="323"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="378"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00029641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF03344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF20920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013925045.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="55025" checksum="987BA6B8814E8E5F" modified="2020-10-07" version="1">MSPRGRPGPPLSNASPAPFPLQFVAFCSKHTEDHPEVISYLSGRHQKTSPEYLASVEFHNVLSRCLTRVQARRNKVYVYINELCTTFRAHSQRKRAVAPGPTDAQDAPAAPAPQTPSEPAKPCVGNKRQIRYLENLLRVYATEIQRLQEQELDLEELDNQDSAYLQESRLKRRLMHVFKRLCQLKDCNSLTGRVIEQRIPYRGTRYPEVNRRIERLINQPEAFPDFADILKVVQKANDRHNLGLPKRQMEVMARDAFRDVGARLQERRRLDLVYNFGSHLTDQYRPNTDPALADVELARHLQRNQALGVQRLEEVIAQFAQLQDKSEEQQQQQGGRGTQSSRAAEGEPASPKESVLGTAAREDSGAGPKESSKTGAPWEEEEEEEEEEEEEEEEEEDSDSESDLEAELAHCLDGGEEEEEEEEEAAPGSLGGTIRPXRTGTRLAAWLGGTSAPAAAHWLGRACPDGSWFESFRRCRGGVSGERRPRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMU7</accession>
    <name>A0A6I9YMU7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vascular non-inflammatory molecule 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">VNN3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925572.1">
        <property type="nucleotide sequence ID" value="XM_014070097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMU7"/>
    <dbReference type="GeneID" id="106551933"/>
    <dbReference type="KEGG" id="tsr:106551933"/>
    <dbReference type="CTD" id="26464"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017159">
        <property type="term" value="F:pantetheine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015939">
        <property type="term" value="P:pantothenate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040154">
        <property type="entry name" value="Biotinidase/VNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043957">
        <property type="entry name" value="Vanin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609">
        <property type="entry name" value="BIOTINIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609:SF27">
        <property type="entry name" value="CN HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19018">
        <property type="entry name" value="Vanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vanin C-terminal" evidence="2">
        <location>
            <begin position="125"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013925572.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31711" checksum="38F9264A9E6A2F68" modified="2020-10-07" version="1">MLNFREPAISLVEKFKVDTILFPTAWLNTLPLLSAVQYYSAWAMGTRVNVLAANIHKPSLDITGSGIFTPNGPKAYHFDMESQSGKLLIAEIEARPRLSPTYPPTFNWSLHASGLPHFSPESLVFHNSIYFDNFTFTELAQEAGNYTVCQKNLCCYLNYSMAEKQEDEVYALGVFDGLHVIEGEYYLQICTLLKCNNTDPKSCGQRVDTAATRFNFFSLSGSFNTNYVFPEVLLSGTQLAPGEFKVLPDGQMISEKGLSKPLLVAALFGRWYEKDSPHPTSTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQT3</accession>
    <name>A0A6I9YQT3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Natural resistance-associated macrophage protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Solute carrier family 11 member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106552710</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Macrophage-specific antiporter that fluxes metal ions in either direction against a proton gradient. Localized to late endosomal lysosomal membranes, delivers bivalent cations from the cytosol into these acidic compartments where they may directly affect antimicrobial activity. Involved in iron metabolism and host natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Fe(2+)(in) + H(+)(out) = Fe(2+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Mn(2+)(in) + H(+)(out) = Mn(2+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73063"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Zn(2+)(in) + H(+)(out) = Zn(2+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:28839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NRAMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926526.1">
        <property type="nucleotide sequence ID" value="XM_014071051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQT3"/>
    <dbReference type="GeneID" id="106552710"/>
    <dbReference type="KEGG" id="tsr:106552710"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051139">
        <property type="term" value="F:metal cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF52">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013926526.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="16645" checksum="33E247AEE4E5AD0D" modified="2020-10-07" version="1">MRWSRFARVVFTRSFAILPTVFVAAFRNVGDLTGLNDLLNVLQSILLPFAVIPVLTFTSMEPLMKDFVNGIIGKFIMILITGLICAINLYFVVTSIISLNYLAYYIGISFVLLAYVAFVCYLIWTCLISHGAVFLTRGPHTRFSYGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR21</accession>
    <name>A0A6I9YR21_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106552878</name>
        <name evidence="15" type="synonym">AARS</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Interacts with ANKRD16; the interaction is direct.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013926747.1">
        <property type="nucleotide sequence ID" value="XM_014071272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR21"/>
    <dbReference type="GeneID" id="106552878"/>
    <dbReference type="KEGG" id="tsr:106552878"/>
    <dbReference type="OrthoDB" id="2423964at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000002">
        <property type="entry name" value="alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF36">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="16">
        <location>
            <begin position="4"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="580"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="584"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="698"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="702"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013926747.1"/>
        </source>
    </evidence>
    <sequence length="801" mass="89097" checksum="2572174ECB30B0B9" modified="2020-10-07" version="1">METLTASEIRQRFINFFVDHQHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTLDPSHPMAKLTRAVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALDLLTKEFGIPIERLYVTYFGGNEAAGLEPDLDCKQIWLDLGLAESKILPGSMKDNFWEMGDTGPCGPCSEMHYDRIGDRDASHLVNQDDPNVLEIWNLVFIQFNRWYVLRRILRRAVRYAHEKLSAPKGFFATLVDVVVQSLGTGCRPYSGQVGEEDVDGIDMAYRVLADHARTITIALSDGGRPDNTGPSRDLTMETLTASEIRQRFINFFVDHQHTYVHSSATIPLDDPTLLFANAGMNQRGASFNTDTLNHPYSVLQKDGELCDTAWLLYDTYGFPVDLTGLIAEEKGLRVDMEAFEEERKTAQLKSQGKGGGDEDLIMLDIYAIEDLRARALEPTDDSPKYNYRSDSDGFYEFGTPGATVKAIRRDKTFVDEVHTGQECGIVLDQTCFYAEQGGQIYDEGYLVKEDGAEDRTEFTVKNVQVRGGYVLHVGTVYGSLKVGDFVGLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGAMSTQEIKKTEEIVNGMIQDAKVVYAKECPLAAAKAIQGLRAVFDETYPDPVRVVSIGIPVDDMLNDPSSAAGAVTSVEFCGGTHLQNSSHAGHFVIVSEEAIAKGIRRIVAVTGAEAQKALRKSEGLIKQLADLETKVKIQTTPNKDVQREIADLGEALATAIIPQWQKDELREALKALKKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YST0</accession>
    <name>A0A6I9YST0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphorylase kinase</fullName>
            <ecNumber evidence="3">2.7.11.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PHKG2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + phosphorylase b = 2 ADP + phosphorylase a.</text>
            <dbReference type="EC" id="2.7.11.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.19" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013926695.1">
        <property type="nucleotide sequence ID" value="XM_014071220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YST0"/>
    <dbReference type="GeneID" id="106552842"/>
    <dbReference type="KEGG" id="tsr:106552842"/>
    <dbReference type="CTD" id="5261"/>
    <dbReference type="OrthoDB" id="419455at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004689">
        <property type="term" value="F:phosphorylase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14181">
        <property type="entry name" value="STKc_PhKG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000138">
        <property type="entry name" value="Phosphorylase b kinase gamma catalytic chain, liver/testis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000149">
        <property type="entry name" value="phosphorylase b kinase gamma catalytic chain, liver/testis isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002291">
        <property type="entry name" value="Phosph_kin_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01049">
        <property type="entry name" value="PHOSPHBKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="50"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013926695.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="50105" checksum="6E393E0F80112F2A" modified="2020-10-07" version="1">MCALECLGLRTSQSESAPVVYQASSTMTRDVGLDDELPDWAGAKEFYQKYDPKDVIGRGVSSVVRRCIHKQTGQEFAVKIIEVTPERMTPQQLEEVRLSTSKEIAILHQVSGHPFIITLIDSYETSTFMFLVFDLMRRGELFDYLTEKVTISEKETRCIMRALLEAVNYLHENHIVHRDLKPENILMNDDLSIKLSDFGFSCHLKPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVIFFTLLAGSPPFWHRKQMLMLRMIMEGRYHFGSPEWDDRSDTVKDLICRLLKVDPVERLSAEEALQHPFFQRYGKEQRHFSPFHKFRVIVWTVRASIRIYSSYRRVRPVTKELLLCDPYALKGVRKLIDACAFRIYGHWVKKGEQQNRAALFENVPKAIQMVLLNTEGEEAPPSEEPFCQKDTFEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT09</accession>
    <name>A0A6I9YT09_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">L-amino-acid oxidase</fullName>
            <ecNumber evidence="2">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106553291</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013927236.1">
        <property type="nucleotide sequence ID" value="XM_014071761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT09"/>
    <dbReference type="GeneID" id="106553291"/>
    <dbReference type="KEGG" id="tsr:106553291"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000521">
        <property type="entry name" value="FAD dependent oxidoreductase domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF23">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026787234" description="L-amino-acid oxidase" evidence="5">
        <location>
            <begin position="26"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013927236.1"/>
        </source>
    </evidence>
    <sequence length="683" mass="78636" checksum="DC291F99E4D39138" modified="2020-10-07" version="1">MVLSFLKCVQELLLIFALSVASSWSNDFLYHDYCIIGAGPSGLQMAYFLQHAGRNYVVFERRNTPGHFFTLYPRHRKLISINKRYTGKSNSEFNLRHDWNSLLSHNNQLLFRHYSQDFFPDADTMVHYLADFASKLALHVQYNTSIVLVMLEKDPKAWNGHYFILRDQNAKNYKCSVLLVATGMWVPHEVNFPGSEYVEGYESVSINPKDFVGQSVLILGRGNSAFETAENILGVTNFVHMVGRSRVRLSWATHYVGDLRAINNGLLDTYQLKSLDGLIEGDLEDLVLIKDRKGKLHITLRFYLENSNSSDPESVTLPQDELDNFATRAPYDRAIRCLGWKFDFSIFNKSVGLMRGKGSKKKYPLIKASYEAKATRGLFLLGTASHSVDFRKSAGGFIHGFRYTARAVHRLLEVRHHKVPWPASNYPVVQLTNAIVKRVNEASGLYQMFSVLADIILLKENATKFEYLEEYPVGVLQDLEWRTGRKVQDGLFVIVMEYGKNFSGPDKDVFYYNRAVGEPEHAWQSNFLHPVIYYYKQLPTEREMRLCPPDWPLPRPDAIHHIVEDFLTDWTAPNAHVLPLRRFLENCLETDLRRFYAESCFLFAFTHLRLPPFCQQEYMKKQGLVGSEKLQHHRVAAELIEHASIKSQDEQESGTMESHNQLLKSTKISSHQLQHLTSTKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUG4</accession>
    <name>A0A6I9YUG4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BRCA1-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BRAP</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927629.1">
        <property type="nucleotide sequence ID" value="XM_014072154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUG4"/>
    <dbReference type="GeneID" id="106553618"/>
    <dbReference type="KEGG" id="tsr:106553618"/>
    <dbReference type="CTD" id="8315"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927629.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="15946" checksum="8C5C1CC5CE952552" modified="2020-10-07" version="1">MSVSLVVLRLELAESSPVPGGFSYSAAATEMSDEGRPETSLAASAVTLEGKVPVERATIIHQHLGRREMTDVIIETIKPDPDEVKEMMDKVGAAEASSTEDHHNRDGQSGSSGTTPDSPSKQLPDQISFFSGNPSVEIAHGIMHLYKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUK9</accession>
    <name>A0A6I9YUK9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger and BTB domain-containing protein 7B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZBTB7B</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927330.1">
        <property type="nucleotide sequence ID" value="XM_014071855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUK9"/>
    <dbReference type="GeneID" id="106553380"/>
    <dbReference type="KEGG" id="tsr:106553380"/>
    <dbReference type="CTD" id="51043"/>
    <dbReference type="OrthoDB" id="10004641at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000115">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000673">
        <property type="entry name" value="Zinc finger and BTB domain-containing 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF4">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="34"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="373"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="429"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927330.1"/>
        </source>
    </evidence>
    <sequence length="481" mass="53418" checksum="803A7D1FEA66B666" modified="2020-10-07" version="1">MGSPEDDLIGIPFPEHSSELLGRLNEQRRRGMLCDVTLRAQGSEYKSHRAVLAACSLYFQRLFAGPSMAVCELDFVEAEALGALLDFAYTATLTISTGSLGEVLRAARILEIPCVIAACMEILQGNGLEAPVPDVAARERARRYLEAFGTLPNMEGDLPEPPARPAARRSKKTRKFLQARGSQLNNHITEISPSLSPSSTGTQERPSPPNQASPVPVLGHPREEDEEVMAPSRTFPFPYNLSPEDLGSEDEPTEQDILTYMRRLNQEGLSVGLDPPDKLVRKRRSQMPQECPVCHKIIHGAGKLPRHMRTHTGEKPFACDVCGVRFTRNDKLKIHMRKHTGERPYSCPHCTARFLHSYDLKNHLHLHTGARPYECCLCHKAFAKEDHLQRHLKGQNCLEVRTRRRRRGPDPETDSLAPSGIALSSAQFGLTNGRVEEQHNYWPAGADARSPAPKEEEEEEEEEGAVEGVQQDGPTAAVQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWU2</accession>
    <name>A0A6I9YWU2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alpha-crystallin A chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106554375</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928499.1">
        <property type="nucleotide sequence ID" value="XM_014073024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWU2"/>
    <dbReference type="GeneID" id="106554375"/>
    <dbReference type="KEGG" id="tsr:106554375"/>
    <dbReference type="OrthoDB" id="8946669at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF2">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="19"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928499.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="16509" checksum="2181366A722CFF93" modified="2020-10-07" version="1">MDSMWELLTSNWRPTASSMVSRETIHSLETPSANDTFAVTQDMTGFDPQELVVKLVGEKVVLTGRKASEMPNGPFRYEVFRRAWDVPQSVDRDRLSCSISSDGQLRIEGPVTESAMRTVPIAVNRVRNEPQPAADEESTHTKDSRAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXK8</accession>
    <name>A0A6I9YXK8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Class I histocompatibility antigen, F10 alpha chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106554243</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928345.1">
        <property type="nucleotide sequence ID" value="XM_014072870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXK8"/>
    <dbReference type="GeneID" id="106554243"/>
    <dbReference type="KEGG" id="tsr:106554243"/>
    <dbReference type="OrthoDB" id="8936120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF242">
        <property type="entry name" value="MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-RELATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0490">MHC I</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="MHC class I-like antigen recognition-like" evidence="10">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013928345.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="11515" checksum="8D410E8F66D5A485" modified="2020-10-07" version="1">MANSSSLHSLKYFYISASDPSQGLPHFVVWGYVDSQLFTLYDSSSRMFQPRASWMEKAEKDYWDTQSQIGHVTEDVYRAALETLRSRHNQSKVLVSVVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXW0</accession>
    <name>A0A6I9YXW0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="2">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106554595</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013928781.1">
        <property type="nucleotide sequence ID" value="XM_014073306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXW0"/>
    <dbReference type="GeneID" id="106554595"/>
    <dbReference type="KEGG" id="tsr:106554595"/>
    <dbReference type="OrthoDB" id="10252326at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="5">
        <location>
            <begin position="9"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="4">
        <location>
            <begin position="106"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928781.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18774" checksum="BC2DCC6DB91EA834" modified="2020-10-07" version="1">MNCVGEKLGRVLSTMTDAILARVYKHCDLITLAEEAAIPVINGLSDFDHPIQILADYLTLQEHYRSLNGLTLSWIGDGNNVLNSILLSAAKFGMNLHVATPKTAQEAASDWTFLHCLPRKPEEVNDEVFYSPKSLVFQETENRKWTIMAVMVSLLTDYSPQMQMPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYQ5</accession>
    <name>A0A6I9YYQ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nicotinamide N-methyltransferase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106555489</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929833.1">
        <property type="nucleotide sequence ID" value="XM_014074358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYQ5"/>
    <dbReference type="GeneID" id="106555489"/>
    <dbReference type="KEGG" id="tsr:106555489"/>
    <dbReference type="OrthoDB" id="10050085at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000065">
        <property type="entry name" value="Phenylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000940">
        <property type="entry name" value="NNMT_TEMT_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10867:SF32">
        <property type="entry name" value="NICOTINAMIDE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10867">
        <property type="entry name" value="NNMT/PNMT/TEMT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01234">
        <property type="entry name" value="NNMT_PNMT_TEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51681">
        <property type="entry name" value="SAM_MT_NNMT_PNMT_TEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013929833.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24657" checksum="268F1AB7CE60458C" modified="2020-10-07" version="1">MEETYTGKDFYAQHFDPRDHLEKYYNVSLVDEGMGQFVTFFLRGAHRAFTVDGIKGDTLIDIGSGASIYQFLSACESFREIIATDCADQNWEEMQRWLKKEPGAFDWTPIVKYVCELEGDRDKWPEKEEKVRRAVKRYLKCDVTQPNPLAPLVLPPADCLVSSLCLDAACKDIPAYRSAFRNISCLLKPGGHLLYYLVWSYTLFKPMVSLVEKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZE5</accession>
    <name>A0A6I9YZE5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Adhesion G-protein coupled receptor G5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106555007</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929246.1">
        <property type="nucleotide sequence ID" value="XM_014073771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZE5"/>
    <dbReference type="GeneID" id="106555007"/>
    <dbReference type="KEGG" id="tsr:106555007"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF326">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013929246.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="29078" checksum="210AF118EB46965A" modified="2020-10-07" version="1">MKVENPAKDRSLKLYHDSTTKIHINLLGALFFLNVSFLISKTLVDHPPWCSILAAFLHYSLLCSLTWMAIEGFHLYLLVIRVYNSYISRYLLKLCAVGWGLPGVIVLVSFLSVKKAYYQHDINTGSQYNNSSICWITSPTLSHITLAYVGGTVLFNMVILVLVVQKLRQLRAHPLRRPQEHTCKDVGTVLGMTFLLGTTWILLFMSFGVFLVPQIFLFTIFNSLQGAIICLWYCFLRCRSASKNSPGFSQSSATSPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z039</accession>
    <name>A0A6I9Z039_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC106555352</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013929659.1">
        <property type="nucleotide sequence ID" value="XM_014074184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z039"/>
    <dbReference type="GeneID" id="106555352"/>
    <dbReference type="KEGG" id="tsr:106555352"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14405">
        <property type="entry name" value="UBA_MARK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF21">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MARK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="60"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="21">
        <location>
            <begin position="329"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="376"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013929659.1"/>
        </source>
    </evidence>
    <sequence length="458" mass="52124" checksum="7E821710BB40C062" modified="2020-10-07" version="1">MSTRTPLPTVNERDTENHTSADGYSESHIQPTKSSSRQNIPRCRNSITSVNEEHPHIGNYRLLKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKCIVHRDLKAENLLLDGDMNIKIADFGFSNEFTIGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPEPDFNDTKRIDLMVTMGFARDEIHDSLINQKYDEVMATYLLLGRKPPECDGNESLSSTTLSQRSRPGSDLNNSSMQSPAHLKVQRSISANQKQRRFSEHGGDGIQPSKVAEYNFKLCSLSWGLGSNTFLYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z099</accession>
    <name>A0A6I9Z099_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucosamine-6-phosphate isomerase</fullName>
            <ecNumber evidence="8">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glucosamine-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106555639</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="8"/>
    <dbReference type="RefSeq" id="XP_013930002.1">
        <property type="nucleotide sequence ID" value="XM_014074527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z099"/>
    <dbReference type="GeneID" id="106555639"/>
    <dbReference type="KEGG" id="tsr:106555639"/>
    <dbReference type="OrthoDB" id="7663298at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000045">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF9">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="9">
        <location>
            <begin position="14"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930002.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="18808" checksum="B22EC66AF9F17BDA" modified="2020-10-07" version="1">MRLVILENYDLASEWAAKYICNCIIQFKPNESRYFTLGLPTGSTPLGCYRKLIDYHKRGDLSFKYVKTFNMDEYVGLPRDHPESYHSYMWNNFFKHIDIDPNHAHILDGNAPDLKAECDAFEKKIEEAGGIELFVGGIGPDGHIAFNEPGSSLVSRTRLKTLAMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0B9</accession>
    <name>A0A6I9Z0B9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60S ribosomal protein L35</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPL35</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929300.1">
        <property type="nucleotide sequence ID" value="XM_014073825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0B9"/>
    <dbReference type="GeneID" id="106555057"/>
    <dbReference type="KEGG" id="tsr:106555057"/>
    <dbReference type="CTD" id="11224"/>
    <dbReference type="OrthoDB" id="528635at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="86"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013929300.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="14510" checksum="67B77D1A97AC7D4D" modified="2020-10-07" version="1">MAKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEEGLKTKKQQRKERLYPPRKYAMKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z122</accession>
    <name>A0A6I9Z122_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Coiled-coil domain-containing protein 37</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CCDC37</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930282.1">
        <property type="nucleotide sequence ID" value="XM_014074807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z122"/>
    <dbReference type="GeneID" id="106555880"/>
    <dbReference type="KEGG" id="tsr:106555880"/>
    <dbReference type="CTD" id="348807"/>
    <dbReference type="OrthoDB" id="10264063at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="157"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="288"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="71"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="171"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="412"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930282.1"/>
        </source>
    </evidence>
    <sequence length="628" mass="73326" checksum="F631B862AC2F5F24" modified="2020-10-07" version="1">MSGPSFASSGSEDSEEIYKIRLVSQQTGDVFTIPSKASSRPGTKSSAPRNTDFLLEQNPFKIPADVDIFLLRDKQKEKARMERERRKTLKVHEKMTCSTLQSSKQSGFKRAIQMEEEAEYKELVAEAERLKALRESISWKIAITKDQVVERETMDDYINQKRQMFLLQYAVTVKKDEIQKLENLAVEEEAKLEKAEEDLEKDAAMFDEFLKENDRNSVQALKIAEKETKAKLEKIIEIRELTAQMMNIQSEIARFEDTLQEYVVFKDFLYLLSPKEWWEDHERRRMKEKANKLPSESKEEKLGLPKGSGKDVQELQEKSRRRTISIAKHSSFDTTNVEPPTPTTQSQILARKDSFKLSRIASRQTLKSQQSRKPSVQIAPSEEKFTPELSDEDDDEVPELFFTDPNQLLQVFTELEEQNLSLIQNSQETEETLDELRHTLETTRNKMDREIEQLKMLAVTLQANIVKEEETAADLELKARVFSFGEYKAEVQDKMLASLYRKVLEVYRRCIGENEANLGTLQMLTVIEHQLDDLLECLERVSPGKIEQAEKSKEKERRMRMREEKVRQQRQLQEERLQRALARAQADVKKKTGRRLIFRSEPPAFKEKEDHDQGLIDKEKEELLYYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1Q0</accession>
    <name>A0A6I9Z1Q0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ORM1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106555814</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an essential role in the homeostatic regulation of sphingolipid de novo biosynthesis by modulating the activity of the serine palmitoyltransferase (SPT) in response to ceramide levels. When complexed to SPT, the binding of ceramides to its N-terminus stabilizes a conformation that block SPT substrate entry, hence preventing SPT catalytic activity. Through this mechanism, maintains ceramide levels at sufficient concentrations for the production of complex sphingolipids, but which prevents the accumulation of ceramides to levels that trigger apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Ceramide-sensitive subunit of the serine palmitoyltransferase (SPT) complex, which is also composed of SPTLC1, SPTLC2/3 and SPTSSA/B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ORM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930204.1">
        <property type="nucleotide sequence ID" value="XM_014074729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1Q0"/>
    <dbReference type="GeneID" id="106555814"/>
    <dbReference type="KEGG" id="tsr:106555814"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000303">
        <property type="term" value="P:regulation of ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018147">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930204.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17685" checksum="1F5CD40480D47E3C" modified="2020-10-07" version="1">MNVGVAHSEMNPNTRVMNSRGIWLAYSISVAVLHIIFLSIPFFSVPVVWTLTNVIHNLVMYWFLHTVKGTPFETPDQGKDRLLTHWEQIDYGTQWTSSRKFLSISPIILYILTSFYTKYDPVHFIINTTSLLSVLLPKLPLFHGVRLFGINQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z207</accession>
    <name>A0A6I9Z207_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Seipin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BSCL2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930056.1">
        <property type="nucleotide sequence ID" value="XM_014074581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z207"/>
    <dbReference type="GeneID" id="106555688"/>
    <dbReference type="KEGG" id="tsr:106555688"/>
    <dbReference type="CTD" id="26580"/>
    <dbReference type="OrthoDB" id="3990054at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930056.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="22572" checksum="391C36FABDEE0107" modified="2020-10-07" version="1">MRLEINPRGECPRFGTAGKSRARLTGVPYANGEGILRKKNKESFVVNFKSGSQKLSPQAERTGGKTGRWYIAVFGKRFWLHEKNSRNMLDGEGRWGNKSPPLRKLGCSLDAAPQGTEGSWISFSAAAAPPPLDSPFKLYGSFYYSYMPTVSYVSPVHYQFRTDCGQAGSELCSFPVANISFIKDSRDQVTDSQRPVDDRCVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2B2</accession>
    <name>A0A6I9Z2B2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106556298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930767.1">
        <property type="nucleotide sequence ID" value="XM_014075292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2B2"/>
    <dbReference type="GeneID" id="106556298"/>
    <dbReference type="KEGG" id="tsr:106556298"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005583">
        <property type="entry name" value="Si:dkey-96f10.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF14">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="3">
        <location>
            <begin position="30"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930767.1"/>
        </source>
    </evidence>
    <sequence length="173" mass="19704" checksum="AF1EA0D6F0D56B79" modified="2020-10-07" version="1">MSVSRCCRGGGGDGAKPERLLQGRAWKVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTKEFNVGQYRRDLVKTYKSFEFFLPDNKEALKIRKKCALAALNDVRRYLCEENGHVAVFDATNTTRERRDTIYKFGEENGFKTFFVESVCVDPEVIAANIVVSRNFRLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2V2</accession>
    <name>A0A6I9Z2V2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">N-terminal EF-hand calcium-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106555915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930326.1">
        <property type="nucleotide sequence ID" value="XM_014074851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2V2"/>
    <dbReference type="GeneID" id="106555915"/>
    <dbReference type="KEGG" id="tsr:106555915"/>
    <dbReference type="OrthoDB" id="289247at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042984">
        <property type="term" value="P:regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039862">
        <property type="entry name" value="NECAB1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178:SF3">
        <property type="entry name" value="N-TERMINAL EF-HAND CALCIUM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="32"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930326.1"/>
        </source>
    </evidence>
    <sequence length="293" mass="33371" checksum="79BE44B3402C6B74" modified="2020-10-07" version="1">MMACTEMLTMCLLSAKHAYRMKATETPQHYDKGLAIFHDIFRRADKNDDGKLSFEEFKNCCADGILNSEELWKLFNDIDRHHSGNLETEKLCDYFTEHLGEYRNVLSALEQLNIAVLSAMDKTKLAYESSSKMEQFVTRFLLRETMNQIQSLQVSLECAADTIEEQSGRERNNMNKSETSIGQHATKRCARRIQKNICYSPTDPYSGMLTKGVCVETDNQWPSEITQLQQLISKLECKNPRLEPLKYNMVCKGTDSHILVAQRQISVAESGLVAFCQILQTYTETTAGQSSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3G9</accession>
    <name>A0A6I9Z3G9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate carboxylase, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930516.1">
        <property type="nucleotide sequence ID" value="XM_014075041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3G9"/>
    <dbReference type="GeneID" id="106556078"/>
    <dbReference type="KEGG" id="tsr:106556078"/>
    <dbReference type="OrthoDB" id="9197799at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.600.10:FF:000001">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="98"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930516.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18623" checksum="FB2D97BB4C4D2528" modified="2020-10-07" version="1">MYPKVFSDFKDFTASFGPVECLNTRLFLEGPKIAEEFEVELERGKTLHIKALALGDLNRAGQREVFFELNGQLRSILVKDTKAMKEMHFHPKALKDVKGQVGAPMPGKVIDIKVKEGGQVEKGHPLCVLSAMKMETVVNSPMSGTVKKIYVKTEMSLEGDDLILEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5I7</accession>
    <name>A0A6I9Z5I7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Guanine nucleotide-binding protein G(K) subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GNAI3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the G-alpha family. G(i/o/t/z) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931474.1">
        <property type="nucleotide sequence ID" value="XM_014075999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5I7"/>
    <dbReference type="GeneID" id="106556950"/>
    <dbReference type="KEGG" id="tsr:106556950"/>
    <dbReference type="CTD" id="2773"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007212">
        <property type="term" value="P:G protein-coupled dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002487">
        <property type="entry name" value="Guanine nucleotide-binding protein G(i) subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003559">
        <property type="entry name" value="Guanine nucleotide-binding protein G(i) subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF230">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12 19" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013931474.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="40647" checksum="418C4259E93DD703" modified="2020-10-07" version="1">MGCTLSAEDKAAAERSRMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEVARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDPGVHACFSRSREYQLNDSASYYLNDLDRISQQSYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIRRSPLTICYPEYPGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z855</accession>
    <name>A0A6I9Z855_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106557283</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013931970.1">
        <property type="nucleotide sequence ID" value="XM_014076495.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106557283"/>
    <dbReference type="KEGG" id="tsr:106557283"/>
    <dbReference type="OrthoDB" id="9950135at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF12">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="5">
        <location>
            <begin position="490"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013931970.1"/>
        </source>
    </evidence>
    <sequence length="666" mass="73408" checksum="059156969C4126DD" modified="2020-10-07" version="1">MSDTEPLHIPALAAASSFAPFWPEAQSWDSFLERFKVFLQAHGLTDLPERRKKGVFLHYCGAEMFATACALSAPSPSHAIPFDVLLSRLKIDYEPAPSRYARRKHFHHCIQREGQSIAQYVAALRTAAMDCEFLDLDDALLEQFIYGLLELNLHQRILSRTELSMKIAVDEARAYEMAGQSTPDLRRFPPSVLASLPPPSYQCQVSHEEPWSDFDASVDRLHSLSLKDTGSCLSCGNRHQRSSCRFRAALCHQCGKKGHIAAVCRSPASAPWASRAPIGHGLASVPSSASRCYAVSEPEGFAPEWQMDATSTTSGSRGKMHLRVLLAGRPCQMELDTSSSRTIVSWDNLKQLFPRFMRKRLSFASCLLRDYQGSPIPVLGCLMLPVQYGRFHGPLPLIVVRNSLISLLGLDWFRAFGLSIAGINSLWLTSDIDLLVAEFADVFSETLACYKGTPISLNLDPLIAPIRLKARRVPLALQAKVDTELDKLISQGIVEPVDHAPWETPIVLPLKSDGSLRVCANYKATVNKALQAHPYPVPVVQHVLHSLGQGKIFAKLDLAQAYQQLPVDDATATAQTIITHWGAFRCTRLQFGISVAPGLFQGLMERLLHGIPGVMPHFDDVLIAAPDQSELISNLRAILSRFXASGLHLKCSKCCLGVSAVDFLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WVQ9</accession>
    <name>A0A6I9WVQ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Caskin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106537413</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013906999.1">
        <property type="nucleotide sequence ID" value="XM_014051524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WVQ9"/>
    <dbReference type="GeneID" id="106537413"/>
    <dbReference type="KEGG" id="tsr:106537413"/>
    <dbReference type="OrthoDB" id="6156898at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000225">
        <property type="entry name" value="caskin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000042">
        <property type="entry name" value="caskin-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033635">
        <property type="entry name" value="ANKS1/Caskin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF11">
        <property type="entry name" value="CASKIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="107"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013906999.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="32144" checksum="4B26D0ADECF0F5A1" modified="2020-10-07" version="1">MQAVKAEDVGAVQKLLQRPKPGKAKLLGSTKRVNVNFQDTDGFSALHHAALNGNTELILLLLEAQAAVDIKDNKGMRPLHYAAWQGKKEPMKLVLKAGSSVNIPSDEGQIPLHLAAQHGHYDVSEMLLQHQSNPCILDNSGKTPLDLACEFGRVGVVQLLLNSNMCAALLEPKPGDITDPNGTSPLHLAAKNGHIDVIRLLLQAGIDINRQTKAGTALHEAALCGKTDVVRLLLDSGINAHIRNTYSQTALDIVHQFTATQASKEIKQMLRDASAALQVRAMKDYCNNYDLTSLHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYW6</accession>
    <name>A0A6I9WYW6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C2orf71 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106538233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908149.1">
        <property type="nucleotide sequence ID" value="XM_014052674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYW6"/>
    <dbReference type="GeneID" id="106538233"/>
    <dbReference type="KEGG" id="tsr:106538233"/>
    <dbReference type="CTD" id="388939"/>
    <dbReference type="OrthoDB" id="8954214at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035845">
        <property type="term" value="P:photoreceptor cell outer segment organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903546">
        <property type="term" value="P:protein localization to photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029352">
        <property type="entry name" value="PCARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22017">
        <property type="entry name" value="PHOTORECEPTOR CILIUM ACTIN REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22017:SF0">
        <property type="entry name" value="PHOTORECEPTOR CILIUM ACTIN REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15449">
        <property type="entry name" value="Retinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="924"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="932"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013908149.1"/>
        </source>
    </evidence>
    <sequence length="1221" mass="136290" checksum="446B24A87FF67773" modified="2020-10-07" version="1">MGCTPSRSDIVKNPETKLMDKRIALVDQGGEGFSFPLLIKGFSSCDTEGIRQEEAQVKDHFFKNLSNEEKDVNFYSSSEDPLLSELDQEEKEIEKIVSETEIAISESMKLNKGKHMAEAIMIKKQKSSESEKVDATMGDKSESNKNQKHKNGKKQRSRQGKPGRPCKSKEKSSPSVCQPEKKVDFPDALVKAHQNAYAYLNPNIGKYEAILCMTDQVTETQLIVQQLVNFLLFRFDGINHLFEEIIEDGEDLLKEVGGNLAWSSGKENPKEQPDLLQQLLQYTVNKMQLLNGTVASLASDVLLESCSFLQSASTSLEEKIKAKQRLDEHLVRIIKNLEESAVGSSNHYVDDMTLYSEDSGIGIDSESVKDLNIIDRHGMETNCETIHRTPCNSRNGISMSQDCVLEKQLKNISYSSPQIRSSISSSEVVSKRRSTFLQHQNILKTFNSDKTHESNSFKQSEFSSISSTNEDNSANSLSEEECDSVSLSEKEKNAVSKRSISFPAAQENRQKASTKQIDSTDNEIILKMKDAISEKITFVPAKCRQRAWIEDENGKEFRRPSTASGGQKTQIKQKRTRSEVSLKSHAEDPTLLELQRTQKDLDRRLELFYQLNGKEEISDTLNPKELFQYFEPVAHRTSTNKLKASLAKNFNILPDSSCRNNENVKCKKPTTSGMSSEDLISRKENYSGTSKPNTPPCKSVKKLIETFSPSESLVKPSNLRTLGPIKCVRKFGLQSISPNLPLPKGFVPINHKPKVSPLEDTSCKNIRTRHCTLSDNSSMEFANSLDINEDSDEIDIENLPPPPPEMLMEMLLESTECSGDIKMEDNYLEEVDKTTRTNEQCTTKKITQISPRMKLSICSLDLLPNKNFSNSPAVNKTPNNEVDTIPNKYVLELSGPYMSDCRKRDDEIEKVADLYKQSCKIIPLPNPKEASESNGSNQGNISNSEAPLPEKQSSPDLLKKNEKTSGFISRVSPVRIPPSSPPNQKRFPSPPLYHRASHQVPSPILHQQPSPSTHSRSASPTVQRKVISPPPQQNLSNPPVIRKQQSSQNQKVSSLPSHEQEPPFGTTPSISPSLFHGCLQNYMNSKDEPHSASPKIVSNAQSIFCPATSSLFEAKPVVSSNINPMGGAMAQPEGSTSARRNSMHLRQLVGQQRMINSAASPQPCLRMSFSDHRPEFQLPLPVFVTAENDPVLNQARPIEKVFSIGLVERTVFPLGDVTVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1J3</accession>
    <name>A0A6I9X1J3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Roundabout homolog 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106538282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013908206.1">
        <property type="nucleotide sequence ID" value="XM_014052731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1J3"/>
    <dbReference type="GeneID" id="106538282"/>
    <dbReference type="KEGG" id="tsr:106538282"/>
    <dbReference type="OrthoDB" id="428111at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07693">
        <property type="entry name" value="IgC_1_Robo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000026">
        <property type="entry name" value="roundabout homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF37">
        <property type="entry name" value="ROUNDABOUT HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="69"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908206.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="25915" checksum="948D8AD91281A304" modified="2020-10-07" version="1">MLRYLLETLLQMNLFADSLAAGDGLANSSELLLSFNSSALAALSPGGFLDLANVTGGNASRPRLEDSSPRIIEHPSDLIVSKGEPATLNCKAEGRPTPIIEWYKDGERVETDKEDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQAPSDVVVAAGEPAVMECIPPRGHPEPTISWKKNSIRISDKEERITVRSSMSAFCTHIANWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1X7</accession>
    <name>A0A6I9X1X7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calglandulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CETN3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the cellular control mechanism of the secretion of toxins from the gland into the venom.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909259.1">
        <property type="nucleotide sequence ID" value="XM_014053784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1X7"/>
    <dbReference type="GeneID" id="106539098"/>
    <dbReference type="KEGG" id="tsr:106539098"/>
    <dbReference type="CTD" id="1070"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000122">
        <property type="entry name" value="Centrin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000301">
        <property type="entry name" value="EF-hand calcium-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF48">
        <property type="entry name" value="CENTRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="97"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="133"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909259.1"/>
        </source>
    </evidence>
    <sequence length="166" mass="19383" checksum="07AC76B26C69BFF2" modified="2020-10-07" version="1">MNLVTRAEFSEKTKRKKRRELTEVQKQEIKDAFELFDTDKDNAIDYHELKVAMRALGFDVKKADVLKILKDYDREGSGKISFADFNEVVTDWILERDPHEEILKAFKLFDDDDSGKISLRNLRRVARELGENMTDEELRAMIEEFDKDGDGEINQEEFIAIMTGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X657</accession>
    <name>A0A6I9X657_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acidic leucine-rich nuclear phosphoprotein 32 family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ANP32E</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AZ1 from the nucleosome: removes H2A.Z/H2AZ1 from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AZ1 in the nucleosome. May stabilize the evicted H2A.Z/H2AZ1-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state. Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Multifunctional protein that is involved in the regulation of many processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the ANP32 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910934.1">
        <property type="nucleotide sequence ID" value="XM_014055459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X657"/>
    <dbReference type="GeneID" id="106540372"/>
    <dbReference type="KEGG" id="tsr:106540372"/>
    <dbReference type="CTD" id="81611"/>
    <dbReference type="OrthoDB" id="2160613at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019212">
        <property type="term" value="F:phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000003">
        <property type="entry name" value="Acidic leucine-rich nuclear phosphoprotein 32 family member A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045081">
        <property type="entry name" value="AN32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375:SF5">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32 FAMILY MEMBER E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="152"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="153"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013910934.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="30393" checksum="33B00AA7666A723F" modified="2020-10-07" version="1">MEMKKLEMKKRVNLELRNRAPEKVAELVLDNCRSGNGEIEGLNDSFKELEFLSMANTELSSLAKLPTLTKLRRLELSDNLISGGLEVLAERCPNLTYLNLSGNKIKDLSTVETLQNLKNLKSLDLFNCEITNLEDYRENVFELLHQITYLDGFDQDDNEAPDSEEEEEDDDDDGDEDEDEDEDKAGPPGEYEENDEDDEASDLGEGEEEEEEEEVGLSYLMKEEIQDEEDDDDYVEERDNEEEDAVVRGEKRKRSPEDEGDDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8M1</accession>
    <name>A0A6I9X8M1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zona pellucida sperm-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106537891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907648.1">
        <property type="nucleotide sequence ID" value="XM_014052173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8M1"/>
    <dbReference type="GeneID" id="106537891"/>
    <dbReference type="KEGG" id="tsr:106537891"/>
    <dbReference type="OrthoDB" id="8880842at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060468">
        <property type="term" value="P:prevention of polyspermy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF4">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907648.1"/>
        </source>
    </evidence>
    <sequence length="83" mass="9560" checksum="103900190AE34481" modified="2020-10-07" version="1">MDFEIASSSTKPDLNLDTLRLRDPSCGPISWSASRDRVHFRVPLNDCGTTLKVVGEKMVYENEVSSFWPDQPPRWISRDSDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9A3</accession>
    <name>A0A6I9X9A3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">La-related protein 4B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LARP4B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912179.1">
        <property type="nucleotide sequence ID" value="XM_014056704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9A3"/>
    <dbReference type="GeneID" id="106541311"/>
    <dbReference type="KEGG" id="tsr:106541311"/>
    <dbReference type="CTD" id="23185"/>
    <dbReference type="OrthoDB" id="10046764at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08031">
        <property type="entry name" value="LARP_4_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12706">
        <property type="entry name" value="RRM_LARP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034900">
        <property type="entry name" value="LARP4B_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058699">
        <property type="entry name" value="RRM_LARP4/4B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF43">
        <property type="entry name" value="LA-RELATED PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26088">
        <property type="entry name" value="RRM_LARP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="5">
        <location>
            <begin position="137"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="433"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="571"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="655"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="444"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="504"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="664"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013912179.1"/>
        </source>
    </evidence>
    <sequence length="715" mass="79371" checksum="46E5D618740D80C1" modified="2020-10-07" version="1">MTSDQDTKVVAEPQVQQVQEVKDSSHLEPAKVSELNPNAKVWGNHMVHVEASGTEGGVSKPWEEVTDHPPDSCKEALEADGDGDKKHQNESLKEHQEPSLSVPLSNQTDMNSLALDHAEYKTVHENTESGGKEDLRPEGQEDLREALKKTLEFCLSRENLASDMYLISQMDSDQYVPIMTVANLDHVKKLTTDMDLIVEVLRSLPLVQVDEKGEKVRPNQNRCIVILREVPESTPIEEVQALFKGENLPKFINCEFAYNDNWFITFESEADAQQAYSYLREDVKTFQGKPIKARIKAKAIAINSFLPKNGYRPVDMNTYTQQRYTTSFAYIPPVYGPQQQFPLYSLIAPQAWSTTHSYIDPSLVAPFPNTGFINGFTAPTFKPTVAPLATLRQYTPRTRNPSKSYLRHTIPTVDRGTGLLESPTIFNLSADRLINGVRSPQPRQPGQNRTRMQSTTSYIKRDVGSGRVESTASDSSPSLGRGRKNTYGYRKKRDDKFVRGQMQSPPPTKPPSPSFELGLSSFPPLPGAAGNLKTEDLFENRLSNVNVSSKEKGISVGTNTVPAGIPRELSLPVSSALPGTSETSPSPSKVLDDPKAMDKQKETQNTQRLCCIVTTASKSVQVNGDLLAQERRKPSYAEICQRILKDSAALQAAKEQKPNAVGCGKEERKPTETLERSRELKSKDQRRPSGRRSPLPPAVGKCLTKEQSTPPKSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAB5</accession>
    <name>A0A6I9XAB5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Galanin receptor type 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106541575</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912539.1">
        <property type="nucleotide sequence ID" value="XM_014057064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAB5"/>
    <dbReference type="GeneID" id="106541575"/>
    <dbReference type="KEGG" id="tsr:106541575"/>
    <dbReference type="OrthoDB" id="5964776at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15097">
        <property type="entry name" value="7tmA_Gal2_Gal3_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000092">
        <property type="entry name" value="Galanin receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000405">
        <property type="entry name" value="Galanin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF31">
        <property type="entry name" value="LOC100125055 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00663">
        <property type="entry name" value="GALANINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="42"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912539.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="39170" checksum="53B15DF4D3ABDC65" modified="2020-10-07" version="1">MSEQEELADLDGLWNSSANQFSSTKIAIPVVFSIIFLLGTVGNSLVLAVLLQNSQRGCNQTHLFIFNLSLADFFFIVFCVPFQATIYSTEGWVFGAFMCKVVHFLIYLTMYASSFTLAAVSVDRYLAIRYPLHSRELRTAYSAVMTTAIIWGLSMIFSGPYLSYYHLVRWEESYICMPGWEGWKRKILETSTFVFGYIIPAVIVCLSYCRTIKHLWISVDPLMDMSESKKSKWKVTKMIIVVTILFCFCWLPYHVVVLHYLYGDFPFNQTTYALRLLSHCMAYANSCLNPIVYALISKHFRKGFKKVFRCLLRRKPWNRVQVVHSILIKPGVEVASTHIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XB76</accession>
    <name>A0A6I9XB76_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein shisa-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SHISA7</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908448.1">
        <property type="nucleotide sequence ID" value="XM_014052973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XB76"/>
    <dbReference type="GeneID" id="106538479"/>
    <dbReference type="KEGG" id="tsr:106538479"/>
    <dbReference type="CTD" id="729956"/>
    <dbReference type="OrthoDB" id="9836398at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774:SF2">
        <property type="entry name" value="PROTEIN SHISA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774">
        <property type="entry name" value="PROTEIN SHISA-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="7">
        <location>
            <begin position="11"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="396"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="449"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908448.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="51887" checksum="F23AC03D1AAE91DF" modified="2020-10-07" version="1">MSTSSRVWPSETCQGYYDVMGQYDAAFNCTTGGYRFCCGTCQYRFCCDDRSRRLEQGKCRDSPQWYATTIPGPATNGPNVPNSKQQSNSTVYVICGVISFTLAVGVGIKFFFSKASRRPHGRELNVPRALVDILRHQAGTTSCSERNNSLVMNSNFQDNGSAHPPKNLYNAVKPSKSSHDNMHHNYIHLNVNSPKHHTATLDWHMNDTPSHSSTKYNTLSCSRSFHNLSHLPPSYESAIKSELNRYSSLKRLAAEKDLDEFYAKRRHLAELTRGTLPLHAMKMNYDRDSYSEKSHNPRRVMSQEHILSDGGNGVGSYRPSHYDYTIPRERVISHERLLSRENLHSQEHLLSPERLHQHTGPFQEMHLGHQKAVSQTNVCASSTPMLDHHHMMMKNSHPTSNNSPKASAPWETSSSSNQAAATRRQGFASKRQSTIDQVQFIPGHHPTQHLPTGSKNEVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCM8</accession>
    <name>A0A6I9XCM8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911607.1">
        <property type="nucleotide sequence ID" value="XM_014056132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCM8"/>
    <dbReference type="GeneID" id="106540859"/>
    <dbReference type="KEGG" id="tsr:106540859"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000389">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT BETA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911607.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="34787" checksum="2D93DC0AF0AA192E" modified="2020-10-07" version="1">MLLELFDSEDPREREYLKTILHRVYGKFLGLRAYIRKQCNNIFLRFIYEMEHFNGVAELLEILGSIINGFALPLKAEHKQFLVRVLIPLHSVKSLSIFHAQLAYCVVQFLEKDATLTEHVIRGLLKYWPKTCTQKEVMLLGEIEEILDVIEPSQFVKIQEPLFKQVARCIASPHFQVAERALYFWNNEYILSLVEDNCHTVLPTIFGTLYRVSKEHWNPTIVSLVYNVLKTFMEMNGKLFDELTASYKVEKQQELKKEKERQELWRHLDELQLQKLQGLEEAKMNRLNLQRTLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCP1</accession>
    <name>A0A6I9XCP1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase</fullName>
            <ecNumber evidence="1">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CHST15</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013913359.1">
        <property type="nucleotide sequence ID" value="XM_014057884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCP1"/>
    <dbReference type="GeneID" id="106542215"/>
    <dbReference type="KEGG" id="tsr:106542215"/>
    <dbReference type="CTD" id="51363"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="252"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913359.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="65280" checksum="9D6B13E8C0EEDE47" modified="2020-10-07" version="1">MRHCIHCCIALLSNDLHKLCFKCRGPSHNIQKCPLCRGENQVLFVDSKQMNLLAVLEVRTDHNDSWTGLFCLKKGKICSLFFGLLIMMLVTASYILTGTKNGLLLMQPTLHYGVFTSNSNLMDNGNFCDKKEHYQKSKMNISYAKDYSSVKVIIDTIASHIEFVSRYPPDLEDLKKEETHMFSVIPNKFLTNSKNPCWYEEYKGNNTINPYATNSYALYSKRFRTIFDYLRKVFWNHLYQYDDKYYRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVKFSAIKEPHWWTRKRFGIIRLRDGFHDRYPVNDYLDLFDLAAHQIQNVLQRDSENSHEKRSNIIIGEASASTMWDNNAWIFFYDNSTEGEPPFLIPDFIHAFQPNAKLIVMLRDPVERLYSDYLYFASANKSVEDFHEKVADSLQLFENCLTDFSLRACVYNNTLNNAMPVRLQVGLYAVFLLDWLAVFNKDQILVLRLEDHASNVKLTMHLVFQFLDLGALSEKQEALITKSPASNTRRPEDRNLGPMLPVTKEILCDFYQPFNKKLAEVLSDEAFLWKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD18</accession>
    <name>A0A6I9XD18_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EIF1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912141.1">
        <property type="nucleotide sequence ID" value="XM_014056666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD18"/>
    <dbReference type="SMR" id="A0A6I9XD18"/>
    <dbReference type="GeneID" id="106541278"/>
    <dbReference type="KEGG" id="tsr:106541278"/>
    <dbReference type="CTD" id="10209"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000003">
        <property type="entry name" value="Eukaryotic translation initiation factor 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="31"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912141.1"/>
        </source>
    </evidence>
    <sequence length="113" mass="12747" checksum="4B6C7F9B53FFEB34" modified="2020-10-07" version="1">MSAIQNLHSFDPFADASKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLIEIGLAKDDQLKVHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDD0</accession>
    <name>A0A6I9XDD0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cingulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CGN</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cingulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911867.1">
        <property type="nucleotide sequence ID" value="XM_014056392.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541064"/>
    <dbReference type="KEGG" id="tsr:106541064"/>
    <dbReference type="CTD" id="57530"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF4">
        <property type="entry name" value="CINGULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="Myosin tail" evidence="7">
        <location>
            <begin position="1065"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1002"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1282"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="260"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="350"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="393"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1309"/>
            <end position="1324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911867.1"/>
        </source>
    </evidence>
    <sequence length="1324" mass="152355" checksum="BFFE0C8446C1A4F6" modified="2020-10-07" version="1">MDHSVNGTLVEKHSPIDYGVQIRFIDDLKEPRRSQKARPKAGKSGSYGVAVRVQGIDGQPFVVLNSGEKGGDSFGVQIKGGNKYERPAQTFEETFLGSPRAGSSSKDISGSISSDSDLPENPYGVKPSRYGSQYSTSDEEPGLQRVPQADSLDGIRVSRTNPSSAAHSGYRTMPHKKSMGQQHLRRTQSHSSLLEPETSEPQGVSHSRTHNLGYSSQSSTSGIPERGSGARNPSPPRLPNLREPTTTNFSGLRPVPKLVSSDTDSPEPTQPIRTNNGDIDTKPLSSVDSLIHKFDGKAPPRGRASRRTRVSPESHQRSRSLDGRSAYRDMDDEREREINRHHPGDFKEVAQSSAAKPLVSANSPGSRGASVQEVKKEKEVDKSKMIQDWVNKSLDEPGTEKQTRKIQSDLQLKSTPDLLRDQQKVAQPGSSEYTKEVIYSILKDRSSDSDAMAKRKTNLIFEKIQAFAAVPSSELRESGAEITKTKLKMDEADSRNKAAQSLPRHLQIRLQELEEECQRLREAFEKKSQELQSTGKELRDVRAVKEQLEAKLTDLEDQLTEMQKALDRFQEVSSASPDQEALLKELLETREDLEEILTAKQKQEDQLRQRERELTALKGALKEEVASHDKELDRVRQEYQQDMEQLRRNMEDVSQDQDSLEAERQKVNSLVRTLQTELQESQEEAAHWKEVSQRNKEELRSVKQELMQVKVEREEFEEDLKESREHLAATRKEMEQLRNRSTDAEGLHKLRKELRQAQEQLQDMAEEKEDQHKVLRLRESEIIDLKEKLDDEVASHSQDLQGLNLRFQEKMQQLQKDYEEAFKTKEALEGEKEAMEQTRKLVESTLQESQEENDDLRRKILGLETQLKEYHRFADSWQDVETRLKEKIFKLEADRKQMEESVNNAADQEQDFLLAKRSLESRLEEAQRNLNRLTLEHQDLSTSYQEELKQKEALRRAKNDLEEEKRLLDRSIVKLSKELKQMTEESHQALSELQSQLEEYKEKSRKEISDSQKQAKDRNAEVEKMQYELGRCQDEVLRLKQALQDSQAELENAVLDKELLAQRFRNLEQEMETKRRSQDDRSRQVKALEDKLKRLEVELDEERNTVELLTERINRSRDQIDQLRAELLQERSARQDLECDKVALERQNKDLKSRLTSNEGLQKSSVNVSQLESRIQELQEKLLAEEREKSVLLSSNRKLERKMKELSIQIDEERQQVNDQKDQLSLRVKALKRQVDEAEEEIERLEAARKKALRELEEQHETNEQLQSRVKALEKDLWRKAARSTAESSLRDDQLSSDEEFDSAYGPSSIASLLTEGNLQTSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFF6</accession>
    <name>A0A6I9XFF6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5'-AMP-activated protein kinase subunit beta-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">LOC106543036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914399.1">
        <property type="nucleotide sequence ID" value="XM_014058924.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914400.1">
        <property type="nucleotide sequence ID" value="XM_014058925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFF6"/>
    <dbReference type="GeneID" id="106543036"/>
    <dbReference type="KEGG" id="tsr:106543036"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="6">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013914399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013914400.1"/>
        </source>
    </evidence>
    <sequence length="109" mass="12247" checksum="9A2B930C6007C1F4" modified="2020-10-07" version="1">MGNTSSERQGGERQGGHKTPRRDSPGGSKEGDRPKILMDSPEDADLYNAEEMKASMEKHEFLAWQHDLEVSDKAPTQAQPTVFRWTGGGKEVYLSGSFNNWNKLPLTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFI0</accession>
    <name>A0A6I9XFI0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ADAMTS-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106539535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909808.1">
        <property type="nucleotide sequence ID" value="XM_014054333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFI0"/>
    <dbReference type="GeneID" id="106539535"/>
    <dbReference type="KEGG" id="tsr:106539535"/>
    <dbReference type="OrthoDB" id="10062690at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF159">
        <property type="entry name" value="PLAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="ADAMTS/ADAMTS-like Spacer 1" evidence="3">
        <location>
            <begin position="17"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013909808.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17132" checksum="D5258E77D10AA565" modified="2020-10-07" version="1">MDRCRVCGGDGSTCYRVSGSFRKGISQLGYIFITNIPAGATDILIIERRKTENILALADDLGHFFFNGNSAIDNPQNFRVAGTVFKYRRPSNLHSDGLEYIIAQGPTNQSLNVMFNLLSTVPRQTVDDFLPLFLESFVDKLCISVQIEITSMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFI3</accession>
    <name>A0A6I9XFI3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106543051</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914424.1">
        <property type="nucleotide sequence ID" value="XM_014058949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFI3"/>
    <dbReference type="GeneID" id="106543051"/>
    <dbReference type="KEGG" id="tsr:106543051"/>
    <dbReference type="OrthoDB" id="9049843at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33066:SF2">
        <property type="entry name" value="FILAGGRIN-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33066">
        <property type="entry name" value="INTEGRASE_SAM-LIKE_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914424.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="27043" checksum="9856DFD6355F02CD" modified="2020-10-07" version="1">DLPQPGGHQSPGPSVAPVGHLALEGELLRKQRFSDQVIRTIQASRRPSTTRIYDATWAAFSQWCKTRDIEASSASIPQTLDFLQEGLDKGLAVSTQRRQVAALSTILARSSSRSLSQHPQIKSFLKGAVNISPPAVHRYPSWDLPFVLKALTKAPFEPLRTTSLRHLTIKTAFLVAITSARRVSELAALSVRQDLCVVHPDRVILRLDPSFTPKINCFTGLKRLFCPTSVPVLLTLRNMSGTSWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFX2</accession>
    <name>A0A6I9XFX2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CCDC25</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts (via cytoplasmic region) with ILK.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912772.1">
        <property type="nucleotide sequence ID" value="XM_014057297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFX2"/>
    <dbReference type="GeneID" id="106541766"/>
    <dbReference type="KEGG" id="tsr:106541766"/>
    <dbReference type="CTD" id="55246"/>
    <dbReference type="OrthoDB" id="200398at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="147"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="147"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013912772.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="24363" checksum="932BA74E536D9016" modified="2020-10-07" version="1">MVFYFTSNVVPSIYTIYMGKDKYENEDLIKYGWLEDIWFHVDKLSSAHVYLRLQKGQTVDDIPKEVLIDCAQLVKANSIQGCKMNNVNIVYTPWANLKKTADMDVGQIGFHRQKDVKIVTVEKKINEILNRLEKTKVERFPDLAAEKEAREREERNEKKAQIQEIKRKEKEEMKKKKELEELRNYSSLMKAENMTSNQDGNDSDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG14</accession>
    <name>A0A6I9XG14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Plasminogen activator inhibitor 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106541800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912812.1">
        <property type="nucleotide sequence ID" value="XM_014057337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG14"/>
    <dbReference type="GeneID" id="106541800"/>
    <dbReference type="KEGG" id="tsr:106541800"/>
    <dbReference type="OrthoDB" id="671595at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF61">
        <property type="entry name" value="PLASMINOGEN ACTIVATOR INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="2">
        <location>
            <begin position="15"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912812.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="27373" checksum="ABE915E9B83A5B5B" modified="2020-10-07" version="1">MAMEALTAANTSFAVDFFKHLCKAQTDTNVLFSPWSISSVMATLYLGAKGHTAQEIAKVFHFNKAGGTETVYPLRHPRVYSKMEELLKNPCISLTKPSPEIHSNIHSRFQALNQEINKPTKNALLSSVNQLYGDKSVSFQRDFSESMKKYYKVEPQTVNFQETTDNARKEINAWIEQQTAGKILNLLNEGSINPLTRLILVNVLYFKGNWLNTFKKEDTIEQPFRLNKVNRYLILYETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG51</accession>
    <name>A0A6I9XG51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IQ domain-containing protein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">IQCC</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913136.1">
        <property type="nucleotide sequence ID" value="XM_014057661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG51"/>
    <dbReference type="GeneID" id="106542047"/>
    <dbReference type="KEGG" id="tsr:106542047"/>
    <dbReference type="CTD" id="55721"/>
    <dbReference type="OrthoDB" id="6161953at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042506">
        <property type="entry name" value="IQCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16049">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16049:SF8">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913136.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24764" checksum="48BDE8AAEFD1485D" modified="2020-10-07" version="1">MREAAAVVRLQAVVRGYLIRKRLQSIRSKYESIIKEIEGDLDWVQWTGHFFPRPAFLSKSAKKSIKQFNREEVKIDKPQKQDAQYFQEVSQSRQMCDFEMQFPSRLLAEVTAEPKIGEVGQQAVDAHSGNACSLPGENEEGGEPSNTSSDWSSTVLGMESPRMSQESHFPRAPEMPRGMPGLQNYRKHLAMELLWLQQAIASRKNYLILRQRLGKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGQ7</accession>
    <name>A0A6I9XGQ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Contactin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CNTN4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910218.1">
        <property type="nucleotide sequence ID" value="XM_014054743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGQ7"/>
    <dbReference type="GeneID" id="106539842"/>
    <dbReference type="KEGG" id="tsr:106539842"/>
    <dbReference type="CTD" id="152330"/>
    <dbReference type="OrthoDB" id="5982258at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000035">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000047">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000052">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000054">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF18">
        <property type="entry name" value="CONTACTIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="93"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="196"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="298"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="395"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910218.1"/>
        </source>
    </evidence>
    <sequence length="521" mass="56880" checksum="06D95AC49F449A2D" modified="2020-10-07" version="1">MDVTAGESILLPCQVSHDHPLDIVFTWSFNGRLIDFEKDGDHFERVGGQDSAGDLMIRSIQLNHAGKYVCMVQTSVDKLSAAADLIVRGPPGPPEAVTIDEITDTTAQVSWRPGADNRSPITTYVIQARTPFSVGWQGVNTVPDVIDGNTFTATVVGLNPWVEYEFRIVAASLIGIGEPSKPSEKRRTEEALPEVTPANVSGGGGTKSELVITWETVPEELQNGGGFGYVVAFRPVGTISWMQTVVVSPDASKYVFRNESLPPFSPYEVKVGVYNNKGEGPFSFITVVYSADEEPTRAPTNVFAKSLSASDIEVFWSLLPETQSKGRIQGYEVRYWKHGDKEEQAGRMKTMTNQTFAKITNLKSSALYHLAVKAYNTAGMGPSSAIVNVTTKKPPPSQPPGNIIWNSSDSKIILNWDQVKAQNNESEVKGYKVFYRWNRQNSMSIIETNKTSVELSLPFDEDYIIEIKPISDGGDGNSSEQIRIPKITNAYARGSGASTSNACTLSAISTIMISLTARSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHB3</accession>
    <name>A0A6I9XHB3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Exonuclease 3'-5' domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EXD1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913491.1">
        <property type="nucleotide sequence ID" value="XM_014058016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHB3"/>
    <dbReference type="GeneID" id="106542325"/>
    <dbReference type="KEGG" id="tsr:106542325"/>
    <dbReference type="CTD" id="161829"/>
    <dbReference type="OrthoDB" id="26838at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990923">
        <property type="term" value="C:PET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06148">
        <property type="entry name" value="Egl_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052144">
        <property type="entry name" value="piRNA_biogenesis_EXD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46628">
        <property type="entry name" value="PIRNA BIOGENESIS PROTEIN EXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46628:SF1">
        <property type="entry name" value="PIRNA BIOGENESIS PROTEIN EXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="2">
        <location>
            <begin position="178"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913491.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="57844" checksum="3B0148F156721292" modified="2020-10-07" version="1">MNTFFKLPKRRLYTWRSLPDARNNITECPIPLREKKNIMDPSCDYHFLHRVLGKTVHITLKCGIFQGILQHVDSSQCIFLNRVKNLENGRNVPGIKRFFGHEIVNVELLDDLEQSAAERNFTPTRSREFPLTKLSEINQGSLLDQSTHTANVVSPVSQQLCLGNVIFKFPDEDSGEDIEYIVINQFQKAFGSAMMHIKNQSVCSIAAEGVNLCRHGKLSWLQVATKCQVFLFDVFVLGAQVFKNGLQMILEDQNILKVIHDCRWLSDCLSHQYGIVLSNVFDTQVADVLQFSTETGGYLPHRISTLQECLMRYFRLPDKCISFLEYKQQAMEEDPEVWFVRPLPPVLLNILALETAYLLPLRLLLLGEIMSDLTTLVDSYLNAFRQGSADPLGSIKISSMELPEELRQLADLYNIRRKTAVQKFQLNEEGFLIRGMGAIKRKKAENEPYPKTYCPDSDTQGNYKKEEIYNHDNQKPIVVNDEIDSKMDIGRDKGENNHKESSVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIC9</accession>
    <name>A0A6I9XIC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rap guanine nucleotide exchange factor 4-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106542463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913672.1">
        <property type="nucleotide sequence ID" value="XM_014058197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIC9"/>
    <dbReference type="GeneID" id="106542463"/>
    <dbReference type="KEGG" id="tsr:106542463"/>
    <dbReference type="OrthoDB" id="21144at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF41">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="43"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913672.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18925" checksum="B0E4D71DD5223A33" modified="2020-10-07" version="1">MVAVHTSHSSSSTEWIACLDKRPLERSSEDVDIIFTRLKEVKAFEKFHPNLLQQICLCGYYENLEKGITLFRQGDIGTNWYAVLTGSLDVKVSDTSNHQDAVTICTLGIGTAFGESVLDNTPRHATIVTRENSELLRIEQKDFKALWEIPKLEQNTIRYGTSVARRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ39</accession>
    <name>A0A6I9XJ39_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Gastrula zinc finger protein XlCGF49.1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106542763</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914056.1">
        <property type="nucleotide sequence ID" value="XM_014058581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJ39"/>
    <dbReference type="GeneID" id="106542763"/>
    <dbReference type="KEGG" id="tsr:106542763"/>
    <dbReference type="OrthoDB" id="10027876at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000739">
        <property type="entry name" value="Zgc:171418 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001478">
        <property type="entry name" value="Zinc finger protein 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_013914056.1"/>
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    <sequence length="155" mass="17744" checksum="5E17F4B243C30E49" modified="2020-10-07" version="1">AEKRGLLGHHKTHTGEKPYQCNDCGRFYSTSSALKSHVKTHTGEKNHKCLECGRTFAYLCSLTSHNRIHTGEKPHKCSDCDKCFSRKDTLVMHQRIHTGEKPNKCLECGKCFAHPSSLLIHTRSHAKERPHKCTKCEKSFHSESDLKKHQKVHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJA0</accession>
    <name>A0A6I9XJA0_9SAUR</name>
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        <submittedName>
            <fullName evidence="10">Drebrin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106542725</name>
    </gene>
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        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
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            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
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            <taxon>Natricinae</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
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        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF12">
        <property type="entry name" value="DREBRIN-LIKE PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
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    </dbReference>
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        <property type="entry name" value="SH3DOMAIN"/>
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    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="167"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="1"/>
            <end position="100"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
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            <position position="1"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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            <dbReference type="RefSeq" id="XP_013914012.1"/>
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    </evidence>
    <sequence length="226" mass="25351" checksum="EB55833705197808" modified="2020-10-07" version="1">EQEEVRNKKGFPRRSESVEKAQEAASLVAQRSVNPREIFKQKERSISTEGAATISSQPGKLRSPFLQERNRVESPASPLPPATQDVPPVVRSSAHDTPPVFPVPELGNSAREENHKAGAATFQPEAEQEVVYEEPPNQHVTYDEPPEPEEANYDYSDTYQENLNTEGKGICARALYDYQAADDTEISFDPENIITNIEMIDEGWWRGYGPDGHFGMFPANYVELIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLI5</accession>
    <name>A0A6I9XLI5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Small glutamine-rich tetratricopeptide repeat-containing protein alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SGTA</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
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            <taxon>Serpentes</taxon>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
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    <dbReference type="OrthoDB" id="2335338at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ALPHA"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00515">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="142"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="4">
        <location>
            <begin position="200"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="4">
        <location>
            <begin position="250"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013916704.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="31972" checksum="406C550E3F1FE321" modified="2020-10-07" version="1">MGAAPTPNPHLLRDFGPRPPACLETAFGVSVDDRGLAISQKLPEIFEAAAAKEPLQGCPVAEPTTPSEEEAAEAERLKTEGNEQMKVENFKSAVSFYGKAIELNPSNAVYYCNRAAAYSKLGNYAGAVRDCERAIGIDPKYSKAYGRMGLALSSLNKLSEAIFYYRKALELDPENETYKSNLKVTEQKMKEAPSPTGSAGGFDLAGLLNNPGFMSMASNLMNNPQVQQLMSGMISSSQSPMAAPGASTAPHDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNEEQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMP6</accession>
    <name>A0A6I9XMP6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acylglucosamine 2-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GlcNAc 2-epimerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">N-acetyl-D-glucosamine 2-epimerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Renin-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106543647</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an N-acyl-D-glucosamine = an N-acyl-D-mannosamine</text>
            <dbReference type="Rhea" id="RHEA:19033"/>
            <dbReference type="ChEBI" id="CHEBI:16062"/>
            <dbReference type="ChEBI" id="CHEBI:17274"/>
            <dbReference type="EC" id="5.1.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19034"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:19035"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-sugar metabolism; N-acetylneuraminate degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Forms a heterodimer with renin and inhibits its activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N-acylglucosamine 2-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.8" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013915182.1">
        <property type="nucleotide sequence ID" value="XM_014059707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMP6"/>
    <dbReference type="GeneID" id="106543647"/>
    <dbReference type="KEGG" id="tsr:106543647"/>
    <dbReference type="OrthoDB" id="414129at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050121">
        <property type="term" value="F:N-acylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010819">
        <property type="entry name" value="AGE/CE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15108">
        <property type="entry name" value="N-ACYLGLUCOSAMINE-2-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07221">
        <property type="entry name" value="GlcNAc_2-epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013915182.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16882" checksum="812C500DE75C3019" modified="2020-10-07" version="1">MRQLNSGLQSQAVDKFMKQPFRSGWDPEHGGLFAFQDVDGFCPTQLEWRMKLWWPHTEAMVAFLMGFAETQDQELLELFHQVANYAFAKFRDPGLAGEWFGYLNQEGKVALTIKGGPFKGCFHVPRALYMCEEILKSLLETKSAIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPZ6</accession>
    <name>A0A6I9XPZ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">N-acetyltransferase ESCO2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ESCO2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. ECO subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912778.1">
        <property type="nucleotide sequence ID" value="XM_014057303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPZ6"/>
    <dbReference type="GeneID" id="106541771"/>
    <dbReference type="KEGG" id="tsr:106541771"/>
    <dbReference type="CTD" id="157570"/>
    <dbReference type="OrthoDB" id="428854at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028005">
        <property type="entry name" value="AcTrfase_ESCO_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884:SF3">
        <property type="entry name" value="N-ACETYLTRANSFERASE ESCO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13878">
        <property type="entry name" value="zf-C2H2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO zinc-finger" evidence="12">
        <location>
            <begin position="411"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="13">
        <location>
            <begin position="569"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="315"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013912778.1"/>
        </source>
    </evidence>
    <sequence length="640" mass="72447" checksum="D8CE1A7BE1C19712" modified="2020-10-07" version="1">MAAVTPRKRKRCYNSLQLVNETPAKKLRMEFTEEASPTKKPSSFQDKKPQNLLERSTSLENLSTSPLQRADAPKVKSNAFPERTSPVSGTIYNSTVPINSFYSKVNTFQSILERKQAKECHINSRNDYANLPIANKTEVIQAKIQPFKTSKRSAVSKQAKTLSRNTKKAKIEMAVPKPKGKENKKYVVEKKIEGSSKVLTMKFKPMLKLQVGAAFFATGKRGNGDSKKVFSPRRSLQFINNTTETKKQDNLLTNTNMHLPSESKAAKTGREENQADVSPKENLDIEDKKTLACNKNHIKDPLDDMKSLPNPRLLQIPHSGSLESPAQKETDVGKEVLEENSKKPSDSTNKYKVTSSNHVHSLFRTFSSNKKSLQTLPDEQHVELSPVRQNSPSKLKINKKTKVLAKGLKDQMIIDAGQKHIGATICKSCGMVYSVANPEDEAHHVQYHQRFMEGIKYTSWKNEQVVAEFWDGKIILILQNDPKYVIKKADDIRKLVDNELGFKHVVQTDPSQTKTYLFVSNEKKIVGCLIAEPVRQAFRVLSEPITEESPTKNSLEHQRAWCCSTKPEKVYCGISRIWVFSLMRRKHIASRLVDVMRQTFLFGCYLSTNEIAFSDPTPDGKLFAAKYCKVPNFLVYNFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ72</accession>
    <name>A0A6I9XQ72_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cerebellar degeneration-related protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CDR2L</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDR2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912853.1">
        <property type="nucleotide sequence ID" value="XM_014057378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ72"/>
    <dbReference type="GeneID" id="106541838"/>
    <dbReference type="KEGG" id="tsr:106541838"/>
    <dbReference type="CTD" id="30850"/>
    <dbReference type="OrthoDB" id="10059415at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026079">
        <property type="entry name" value="CDR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19232">
        <property type="entry name" value="CENTROCORTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19232:SF10">
        <property type="entry name" value="CEREBELLAR DEGENERATION-RELATED PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="105"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="192"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013912853.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="54704" checksum="C5C6249ED95DD2F6" modified="2020-10-07" version="1">MLSADRMDEFQSEEEEPWYDQQDLEQDLHLAAELGKTLLERNKELEDSLQQMYTTNEEQVQEIEYLTKQLEMLRHMNEQHAKVYEQLDLTTRDLELANQKLVLESKASQQKIQCLTETIEGLQNQVDELQKQVEELQSLDQLRVRREKRERRRTIHTFPCLKELCSSPRYEDAFQIHSSSMEFNQMPLERENERLQAMVNSLRSQVIQEKQRKERVEREYTSVIQEYSELEQRVCEMESCKLRIKELETELLELQQMKQVKKYLLSREDNLSETLLEPLNNAPESDYIDLSEEDGRKNHESSMAASPNHPVRKSCSDTALNAIVTKDAVSRHEGNYTLHANNVRKRGMSILREVDEQYHALLEKYEELLSKCRQHKDSVRHAGVQTSRPISRDSSFRDFRGENYELEELKTMEKSLSKHVEAVDKRLEQSQPEYKALFKEIFSRIQKTKADINATKVKKKTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQB5</accession>
    <name>A0A6I9XQB5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Wolframin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">WFS1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916237.1">
        <property type="nucleotide sequence ID" value="XM_014060762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQB5"/>
    <dbReference type="GeneID" id="106544483"/>
    <dbReference type="KEGG" id="tsr:106544483"/>
    <dbReference type="CTD" id="7466"/>
    <dbReference type="OrthoDB" id="5865303at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03493">
        <property type="entry name" value="SQR_QFR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026208">
        <property type="entry name" value="Wolframin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045400">
        <property type="entry name" value="Wolframin_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045460">
        <property type="entry name" value="Wolframin_EF-hand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026209">
        <property type="entry name" value="Wolframin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045461">
        <property type="entry name" value="Wolframin_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045458">
        <property type="entry name" value="Wolframin_Sel1-like_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13098">
        <property type="entry name" value="WOLFRAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13098:SF3">
        <property type="entry name" value="WOLFRAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20053">
        <property type="entry name" value="WC-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19913">
        <property type="entry name" value="WCOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19914">
        <property type="entry name" value="WEF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20023">
        <property type="entry name" value="WSLR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02060">
        <property type="entry name" value="WOLFFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02061">
        <property type="entry name" value="WOLFRAMIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="550"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Wolframin EF-hand" evidence="4">
        <location>
            <begin position="94"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Wolframin cysteine-rich" evidence="5">
        <location>
            <begin position="584"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Wolframin OB-fold" evidence="3">
        <location>
            <begin position="688"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013916237.1"/>
        </source>
    </evidence>
    <sequence length="808" mass="92238" checksum="3952CA943ED5783E" modified="2020-10-07" version="1">MDAPGFEELLARAKAGEPKSQTEVGKHYLKLAEEQDEELNSCSAVEWLIPAAKQGQKEALQILRTCLAEGKGITSENVQEVKQLLSETNLERAVRKAALVIYWKLNPKKKKELAVSELLENVGEVDSEGQGDQPPGPLPKPVQKQRRMLERLVSSEAKNYMALDDFVEITKKYAKGIIPSSMIPQDDDDDELTGKSPEELPFQQKIIKYPLHAIMEIKEHLIEAASKAGMHWLSTIVPTHQINALVFFFVLSNLTIDFFAFFIPLVIFYLSFVSMVICTLKVFQDSKAWENFRTLTDLLLRFEPDLDVDEAEGNFGWNHLEPYFHFLLSVLFVLFSFPIASKEWMPCSELATVSVFFMVTSYMSLCTSAEPYTRRALLTEVTAGALCLLQGAPDHWGYVKLLGKTFYTLPLGSVLVLNLSLPCLLFLYLFYLFYRMARLKKFKGIYCYLIPYLVCFMWCELSVVILQESTSLGLLRASVGYFLFLFALPVLMLGIALMGLFQFLRWLITLELTKIVVTLTLCAVPLFFHLCTRARVSAVEMVTSLTKSSIVKLILVWLSAIVLFCWFYVYRSEGMKVYNSTLTWNQYGFLCGPRAWKETNMARTQIICSHLEGHRVTWTGRFKYVRVTSIENSAESTINMLPYFLGDWLRCLYGETYPACDPRNATLEVEELCRLKYLTKHQCHIKRFDRYKFEITVGMPLGGGNGNRAPEEDDVTKDIVLKASNEFKQVLLNLRQGSVVEFSTILEGRLGSKWPVFELKALSCLNCLSKLSPAGRHVKVEHDWRNTVQKAFRFAFNFLFSPFLSVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR74</accession>
    <name>A0A6I9XR74_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">U1 small nuclear ribonucleoprotein C</fullName>
            <shortName evidence="9 10">U1 snRNP C</shortName>
            <shortName evidence="9 10">U1-C</shortName>
            <shortName evidence="9 10">U1C</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 14" type="primary">SNRPC</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. snrpc/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates E complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the U1 snRNP. The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 3 U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. SNRPC/U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA. Interacts (via N-terminus) with TIA1 (via C-terminus); thereby promoting spliceosomal U1 snRNP recruitment to 5' splice sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">U1 snRNP is composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 3 U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. SNRPC/U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the U1 small nuclear ribonucleoprotein C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907210.1">
        <property type="nucleotide sequence ID" value="XM_014051735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR74"/>
    <dbReference type="GeneID" id="106537565"/>
    <dbReference type="KEGG" id="tsr:106537565"/>
    <dbReference type="CTD" id="6631"/>
    <dbReference type="OrthoDB" id="76567at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000059">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03153">
        <property type="entry name" value="U1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017340">
        <property type="entry name" value="U1_snRNP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148:SF1">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037969">
        <property type="entry name" value="U1_snRNP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="12">
        <location>
            <begin position="4"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013907210.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="17363" checksum="0189E761A0F8FB67" modified="2020-10-07" version="1">MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVKDYYQKWMEEQAQSLIDKTTAAFQQGKIPPTPFSAPPPGGAMIPPPPNIPGPPRPGMMPAPHMGGPPMMPMMGPPPPGMMPVGPAPGMRPPMGGHMPMMPGPPMMRPPSRPMMVPARPGMARPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSK4</accession>
    <name>A0A6I9XSK4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Methyl-CpG-binding domain protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MBD1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916721.1">
        <property type="nucleotide sequence ID" value="XM_014061246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSK4"/>
    <dbReference type="GeneID" id="106544851"/>
    <dbReference type="KEGG" id="tsr:106544851"/>
    <dbReference type="CTD" id="4152"/>
    <dbReference type="OrthoDB" id="10072024at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008327">
        <property type="term" value="F:methyl-CpG binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396:SF57">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MBD" evidence="11">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="12">
        <location>
            <begin position="235"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="12">
        <location>
            <begin position="398"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="315"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="462"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013916721.1"/>
        </source>
    </evidence>
    <sequence length="761" mass="85382" checksum="5BBE434ACDC0E6E1" modified="2020-10-07" version="1">MTDGWVDCPSLGPGWKRREVLRKGGTRSGHSDTYYISPRGEKIRSRVELLRLLGCSRDLTDFDYRRGIFVEPNTEPLSSALFSPTEGSPQRVKKSSKKHLLHLAATTLPSKKRPCLQVFLEPQPSPGQGLLEQKPSEPELEFLGENQANMQSQQSALPVTQDQPAPKPGKRHQHPLPLIPSPGTIEDVTLQESNPKEAMACCASCQGQFPEIMLPSQRRCRWLCPDCRAQRRAFNREQRYYKGLDCGICQACEITEDCGTCAICLRRQRPGMKRQWKCVKRRCLERKKKISPKKDSCSSRKVIAETSAKNVFTPKQCGQKKPTLTSGGQFPKAQLAPGHPHRRQSATPKWKPTIQRDPEGNPLVRHTKKQLGVAKERKKVGRPPKHPVAKLKKSVHSSKNRQKRKCGECEACLLKTDCGRCDFCSDKPKFGGQNLKQQKCRWRQCLQFPMESSLISEWPEVPKSLGSEMPKQEKLHLQPQPQPPPPVKLKKETMSSASHQPTKECSVVPAYIQQEQAILHRPILPSAILGKDPDTKLPVINLLIATSPKNKQEYAEPGGNFQASSDSLVSARFPQFKKQSGTPSTEVVVLDDPEDDEMELLQQIQPPVIMEVYSLGGVQPLSQLDSVLREFLAELNEIPLPAHWEVLPLLGSPDLRLVQRSKHSTMSAAVIHIRPGLFFHVVVQDLLVPQEHKLYASHPARLTTVDEVVELICDLEAYQLCLGWPAGWHARQRSETCDILVHSGCCPQCRLNPWPSGSGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSZ9</accession>
    <name>A0A6I9XSZ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c oxidase subunit NDUFA4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106545238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917212.1">
        <property type="nucleotide sequence ID" value="XM_014061737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSZ9"/>
    <dbReference type="GeneID" id="106545238"/>
    <dbReference type="KEGG" id="tsr:106545238"/>
    <dbReference type="OrthoDB" id="8996531at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256:SF1">
        <property type="entry name" value="GEO09626P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013917212.1"/>
        </source>
    </evidence>
    <sequence length="78" mass="8992" checksum="664A202E12CA2CB0" modified="2020-10-07" version="1">MSFRMRQLTRFPTLSLILITGFGVAGGLYILARKTFFVPEVSWEKNQESWNNLGPSDQYKLFAMSVDSKDLEKDRPDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XU93</accession>
    <name>A0A6I9XU93_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">steroid 11beta-monooxygenase</fullName>
            <ecNumber evidence="4">1.14.15.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome P450C11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106545562</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013917642.1">
        <property type="nucleotide sequence ID" value="XM_014062167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XU93"/>
    <dbReference type="GeneID" id="106545562"/>
    <dbReference type="KEGG" id="tsr:106545562"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047783">
        <property type="term" value="F:corticosterone 18-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004507">
        <property type="term" value="F:steroid 11-beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032342">
        <property type="term" value="P:aldosterone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF1">
        <property type="entry name" value="CYTOCHROME P450 11B2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="9 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 16" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013917642.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="42438" checksum="7579410FA69DAEC5" modified="2020-10-07" version="1">MNGGTEVLHGARPHLLGVTLSCRNGEEWRSDRLILNKEVISPAGTRKFLPFLNTVAEDFVAFMHRQVRKNSRGSLTVDLYHDLFRFTLEASSYALYGERLGLLEESPKPESQEFISAVETMLQTTLPLLFTPPGVMRWVNNRLWQEHLKAWDTIFNHADKCIQNIYQEFCLGKPRKYSGIMAELLVQAELPLDSIKANVTELTAGGVDTVSAPLGHTGYPQGGGTRMLRHGLRGSRGEAAASLFSALSSRLYPVGITIQRYPLRDVVLQNYCVPAGTLCQVALYSMGRSPAVFKDPEQYQPSRWLGKEDNSFKALAFGFGSRQCIGRRLAEAEMTLFLMHILKNFKIDTVSKEDLHTIYSFILKPEKPPRLTFRPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUX6</accession>
    <name>A0A6I9XUX6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Triple functional domain protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106545752</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917882.1">
        <property type="nucleotide sequence ID" value="XM_014062407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUX6"/>
    <dbReference type="GeneID" id="106545752"/>
    <dbReference type="KEGG" id="tsr:106545752"/>
    <dbReference type="OrthoDB" id="10256089at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000008">
        <property type="entry name" value="rho guanine nucleotide exchange factor 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF104">
        <property type="entry name" value="TRIPLE FUNCTIONAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16609">
        <property type="entry name" value="SH3-RhoG_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="6">
        <location>
            <begin position="245"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="203"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917882.1"/>
        </source>
    </evidence>
    <sequence length="424" mass="47967" checksum="318C72D2B4EB72B0" modified="2020-10-07" version="1">MEMEGIFNHKDSLSVSSNDASPPASVTSLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADTGSQKDSDDSAATPQDETLEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGPDAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLMVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRQYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHREYVWFLFLSKFHTVFNLFTLYIFSALHIYRSFLSFQERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKANLDTTELEQMQIRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVK0</accession>
    <name>A0A6I9XVK0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Calsenilin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106545708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917826.1">
        <property type="nucleotide sequence ID" value="XM_014062351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVK0"/>
    <dbReference type="GeneID" id="106545708"/>
    <dbReference type="KEGG" id="tsr:106545708"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF165">
        <property type="entry name" value="CALSENILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013917826.1"/>
        </source>
    </evidence>
    <sequence length="160" mass="17696" checksum="0B20FE9EE4589901" modified="2020-10-07" version="1">MRLEDNTIEKAISFVFKLYQIGLQVIMECPSGLVDEETFKLIYAQFFPQGAVRDVSVGRPGTECPLLPGSQAVTDLPLSFPSPSLCCCLEQDFVIGLSILLRGTVHEKLSWAFNLYDINKDGYITKEVLAKSYFYTQVDLAADSASEDFGEASPGVWGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWR4</accession>
    <name>A0A6I9XWR4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">WD repeat domain phosphoinositide-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">WIPI1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920629.1">
        <property type="nucleotide sequence ID" value="XM_014065154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWR4"/>
    <dbReference type="GeneID" id="106547888"/>
    <dbReference type="KEGG" id="tsr:106547888"/>
    <dbReference type="CTD" id="55062"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000145">
        <property type="entry name" value="WD repeat domain phosphoinositide-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013920629.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="48974" checksum="0C72D9C78EBD1C9E" modified="2020-10-07" version="1">MEAAGGEGEREALTCFSYNQDCTSLAIGTKTGYRLFSLSSVEQLDQVHESNEMPDVFIVERLFSSSLVVVVSRAKPQQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLIVCLEESIYIHNIKDMKLLKTILNTPPNATGLCALSINHSNSYVAYPGSPVVGEIVLYDGNLLRDVCSISAHEGPLAALAFNSTGSKLASASEKGTVLRVFSIPEGQKLYEFRRGMKRYVNISSLVFSMDSQFLCASSNTETVHIFKLEHLTDSRPEEPPSWSGYMGKMFMAATNYLPSQVSGMMSQDRAFATVRLNFSGQRNICVLSTIQKLPRLLVTTSGGHLYIYNLDPQDGGECVLIKKHSLLDLVKPEENKENDLLSPVTQTYAATVARQTSVPSPSMMPGYSEDGGALRGEIIPEHEFATGPVCLDDENEFPPIILCRGSRTNKVKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX40</accession>
    <name>A0A6I9XX40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">USP50</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013918732.1">
        <property type="nucleotide sequence ID" value="XM_014063257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XX40"/>
    <dbReference type="GeneID" id="106546391"/>
    <dbReference type="KEGG" id="tsr:106546391"/>
    <dbReference type="CTD" id="373509"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF11">
        <property type="entry name" value="INACTIVE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013918732.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22848" checksum="1D9ACBC112AFCC23" modified="2020-10-07" version="1">MASRSTNMPEEFNIYYLLKGAFPWQTSEESMDVSRDDTNPQYQGLTGLRNLGNTCYMNAVIQCLCSVSPLVEYFLSGKYVASLDKNHFGDLDGGHYTAFCRHTLSQNWYNFDDSQINEIPESSVQTSAAYLLFYSSQAFSAPVKNQNVFDCQGSYKKKLKTDIYYPLNNLDLSPYIYPLFRKNPKYNLFAVVVSDSSILPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXG2</accession>
    <name>A0A6I9XXG2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calcium homeostasis modulator protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CALHM3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918496.1">
        <property type="nucleotide sequence ID" value="XM_014063021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXG2"/>
    <dbReference type="GeneID" id="106546216"/>
    <dbReference type="KEGG" id="tsr:106546216"/>
    <dbReference type="CTD" id="119395"/>
    <dbReference type="OrthoDB" id="5978124at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029569">
        <property type="entry name" value="CALHM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261:SF7">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14798">
        <property type="entry name" value="Ca_hom_mod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013918496.1"/>
        </source>
    </evidence>
    <sequence length="334" mass="38972" checksum="BE962AC44CD83F10" modified="2020-10-07" version="1">MDRFQMVFQYFQSNSESVTNGICGLLALASVKIYTIFDFNCPCLAKYNMTYGLGIMFIPPVALFFCGLIVNRQFVMMLEEWRRPTGDRRKKPSIIRYMCSSVMQRSMIAPTVWILVTLLDGKCFVCAFSNSIDPEKFPDFAANITTYDALQHFLAKIPCKNDELMRNNTSRKAVSRYLKCCSQAFGWSLFLILIVMAFLVRALKPCFNQAAFLQTRYWSNYIDLEQKIFEETCCEQTRDFAHKCILHFFESMQTEIKLRRFNAYTKEKGEKGKENIHLRGITSEEQISKFLLSWYNRKPPLDFNQAYRQRDDTFALGLGKLNRPIRNFNSGDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXY1</accession>
    <name>A0A6I9XXY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription factor IIH subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Basic transcription factor 2 62 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">General transcription factor IIH polypeptide 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">TFIIH basal transcription factor complex p62 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">GTF2H1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918681.1">
        <property type="nucleotide sequence ID" value="XM_014063206.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918682.1">
        <property type="nucleotide sequence ID" value="XM_014063207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXY1"/>
    <dbReference type="GeneID" id="106546352"/>
    <dbReference type="KEGG" id="tsr:106546352"/>
    <dbReference type="CTD" id="2965"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000115">
        <property type="entry name" value="General transcription factor IIH subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="15">
        <location>
            <begin position="99"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="15">
        <location>
            <begin position="179"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013918681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013918682.1"/>
        </source>
    </evidence>
    <sequence length="548" mass="62104" checksum="3DF4556ECE97A986" modified="2020-10-07" version="1">MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNLNASDNSAAPNKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKLKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNAGSKDLFAECAKMDEKGLKAMVCQGVKNPLLDLTALEDKSLDEGYGVSLAPSTSNSTKSVKESSNAAIIKRFNHHSAMVLAAGLRKPEAQNDHYNETSSIDGNSRDSDIFQPPVKKAKLQESIEYEDLEHNNSIKTIALNLKKSDRYYHGPTPIQSQQYATSHDIINSLCSIREEMEGYVPKLTQVLSSGAASSTITALSPGGALMQGGTQQAINQMLPNDVQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMQSNLERFQVTKLCPFQEKIRKQYLSTNLVNHIEEMLQTAYNKFHTWQSRRMLKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0F0</accession>
    <name>A0A6I9Y0F0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Fez family zinc finger protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FEZF2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916408.1">
        <property type="nucleotide sequence ID" value="XM_014060933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0F0"/>
    <dbReference type="GeneID" id="106544609"/>
    <dbReference type="KEGG" id="tsr:106544609"/>
    <dbReference type="CTD" id="55079"/>
    <dbReference type="OrthoDB" id="5062908at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000103">
        <property type="entry name" value="FEZ family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000251">
        <property type="entry name" value="FEZ family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000227">
        <property type="entry name" value="fez family zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000164">
        <property type="entry name" value="Fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000194">
        <property type="entry name" value="Fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000863">
        <property type="entry name" value="fez family zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013916408.1"/>
        </source>
    </evidence>
    <sequence length="477" mass="50722" checksum="1FDCBF8F42E468CA" modified="2020-10-07" version="1">MASSASLETVMPSACIRTGAANPAKSLAFSIERIMAKGSEPHHKPAAAAFEARPSDSPAKKLLSLCSPLPGVIPVQPLPAYEMPSKALLNYSELWKGSLRHCGAAALGGSTGAFCKSNCGACCKGELPPPPPLGQAALPGAGRVIKPQVIHQTLALPANGSSLYYFNCLDSSAYHPSEILQGQLFASGLLHSPSPAAAAAAAAAAALSAHQKLFLLENAKLAALAPPEKFPNPQYPHKERLPGQLDQVMKENSSLAVERPSGKAHHHAKLGGGSSAGSGGGGTSADGKPKNFTCEVCGKVFNAHYNLTRHMPVHTGARPFVCKVCGKGFRQASTLCRHKIIHTQEKPHKCNQCGKAFNRSSTLNTHIRIHAGYKPFVCEFCGKGFHQKGNYKNHKLTHSGEKQYKCSVCSKAFHQIYNLTFHMHTHNDKKPFTCLTCGKGFCRNFDLKKHVRKLHDPAAAAAAAVPSSKELSRTGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0H6</accession>
    <name>A0A6I9Y0H6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL43</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L43, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MRPL43</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL43 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920307.1">
        <property type="nucleotide sequence ID" value="XM_014064832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0H6"/>
    <dbReference type="GeneID" id="106547605"/>
    <dbReference type="KEGG" id="tsr:106547605"/>
    <dbReference type="CTD" id="84545"/>
    <dbReference type="OrthoDB" id="88at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000078">
        <property type="entry name" value="39S ribosomal protein L43, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039927">
        <property type="entry name" value="Ribosomal_mL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="9">
        <location>
            <begin position="35"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013920307.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17220" checksum="D0DB824E64B1AB4C" modified="2020-10-07" version="1">MTGRGTASRFLASVLRNGVGRYVCQLQRITLTVSQHMSNSLGAREYVEEKIVDFARQNPGIVVYVNPKNCRIPTLVAEYLNGTVKEEPLTSKTAEEIADIVQKMANQSGLEIIRIRKPFHTDNPSIQGQWHPFTNKPSVLNVQTIGARLQSLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y128</accession>
    <name>A0A6I9Y128_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SLC25A4</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910450.1">
        <property type="nucleotide sequence ID" value="XM_014054975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y128"/>
    <dbReference type="GeneID" id="106540012"/>
    <dbReference type="KEGG" id="tsr:106540012"/>
    <dbReference type="CTD" id="291"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000002">
        <property type="entry name" value="Putative ADP/ATP translocase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF32">
        <property type="entry name" value="ADP_ATP TRANSLOCASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="14 17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="7 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="6"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="111"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013910450.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="28548" checksum="A0E00BE99DB0A269" modified="2020-10-07" version="1">MGDQALSFLKDFLAGGIAAAISKTAVAPIERVKLLLQVQHASQQITAEQQYKGIMDCVVRIPKEQGIISFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGLKERQFTGLGNCIAKIYKSDGLRGLYLGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNVHIIVSWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGAFTSVFFRKNAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1C5</accession>
    <name>A0A6I9Y1C5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cbp/p300-interacting transactivator 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CITED2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CITED family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910580.1">
        <property type="nucleotide sequence ID" value="XM_014055105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1C5"/>
    <dbReference type="GeneID" id="106540097"/>
    <dbReference type="KEGG" id="tsr:106540097"/>
    <dbReference type="CTD" id="10370"/>
    <dbReference type="OrthoDB" id="10025072at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060972">
        <property type="term" value="P:left/right pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007530">
        <property type="term" value="P:sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2200:FF:000001">
        <property type="entry name" value="Cbp/p300-interacting transactivator 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007576">
        <property type="entry name" value="CITED"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17045:SF7">
        <property type="entry name" value="CBP_P300-INTERACTING TRANSACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17045">
        <property type="entry name" value="MELANOCYTE SPECIFIC GENE RELATED CITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04487">
        <property type="entry name" value="CITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910580.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="26026" checksum="B55BB99F68D94591" modified="2020-10-07" version="1">MAEHMMAMNHGRFPDGTTGLHHHPAHRMGMGPFPTHHHQQQQPHTFSALMSDHLHYGGGNMNTNSGIRHAMGPGNVNGGHPAGSMPPTARFNNSQFMAPTVAPQGGPLTASMQLQKLNNQYFSHHPYPHNHYMPDLLPANHQLNGATQHFRDCNPKHSSSGAGGGNGSVPASVPHVPAAMLPPNVIDTDFIDEEVLMSLVVEMGLDRIKELPELWLGQNEFDFMTDFVCKQQSSRVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1U6</accession>
    <name>A0A6I9Y1U6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NEDD4-binding protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">N4BP2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916983.1">
        <property type="nucleotide sequence ID" value="XM_014061508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1U6"/>
    <dbReference type="GeneID" id="106545054"/>
    <dbReference type="KEGG" id="tsr:106545054"/>
    <dbReference type="CTD" id="55728"/>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056718">
        <property type="entry name" value="DUF7816"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052772">
        <property type="entry name" value="Endo/PolyKinase_Domain-Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535:SF1">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25124">
        <property type="entry name" value="DUF7816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="1">
        <location>
            <begin position="262"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916983.1"/>
        </source>
    </evidence>
    <sequence length="341" mass="38565" checksum="D3084F9BD028788A" modified="2020-10-07" version="1">MICSKDPMLLERLHPGKVDGVLSQYTADFQEQESLPGAGSAETSAASDVFPYMDHWNVHTQRVSLREIMSEEIALQERLTVKPSPCIAKKDCAAKLKEKQLLELFPTISANFLMDIFKDNNYSLEQTVQFLNNVLEADPIKTVIAKEPAQNARRSPSIASKTREKKAKKTKELEDILSEKGFQDTQYPGYKDFRAEAFLHQQQRQECLRKAGEAYRMGMKPVAAFYVQQGQLHEEKMKEANEDAAQQIFETVNASKLPINLLDLHGLHVDEALSHLSRVLQEKTKEYSLAGGIPYLYVITGRGNHSQGGVARIKPAVMKYLTSHKFRFTEIKSGCFKILLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1Z6</accession>
    <name>A0A6I9Y1Z6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17 18">Erlin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17 18" type="primary">LOC106548202</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17 18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921012.1">
        <property type="nucleotide sequence ID" value="XM_014065537.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013921013.1">
        <property type="nucleotide sequence ID" value="XM_014065538.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013921014.1">
        <property type="nucleotide sequence ID" value="XM_014065539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1Z6"/>
    <dbReference type="GeneID" id="106548202"/>
    <dbReference type="KEGG" id="tsr:106548202"/>
    <dbReference type="OrthoDB" id="77368at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033294">
        <property type="entry name" value="Erlin1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15351">
        <property type="entry name" value="ERLIN (ER LIPID RAFT ASSOCIATED PROTEIN) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15351:SF4">
        <property type="entry name" value="ERLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636626" evidence="13">
        <location>
            <begin position="18"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="14">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013921012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013921013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013921014.1"/>
        </source>
    </evidence>
    <sequence length="79" mass="8310" checksum="5E2C6E1C20488AD6" modified="2020-10-07" version="1">MTQLGAIAALAASLVAASLFSAIHKIEEGHIGVYYRGGALLTSTSGPGFHLMLPFITSYKSVQTTLQTDEVKNVPCGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y218</accession>
    <name>A0A6I9Y218_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106547808</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920541.1">
        <property type="nucleotide sequence ID" value="XM_014065066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y218"/>
    <dbReference type="GeneID" id="106547808"/>
    <dbReference type="KEGG" id="tsr:106547808"/>
    <dbReference type="OrthoDB" id="5964498at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15229">
        <property type="entry name" value="7tmA_OR8S1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013920541.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="34350" checksum="079D5891818A4AA3" modified="2020-10-07" version="1">MENKTTVTEFLLLGLSDDPTTQVFLFLVFSSIYLITLVGNMIIILVIRADPQLHTPMYFFLINLSFLDICYSSVTVPKMLQNLLATKKTISLSGCIAQVFFFIFMIGTEIFLLSAMAYDRYAAICYPLRYNTIINHRLCIQMVLVAWLSGFLDSCVNTLFLTNLYFCGPSNITHFSCELPLLLQLSCTSTFANEMVILIFSMPLGFAALLLIIASYVRIVGSIVKINSAKGRQKVFSTCASHLTVVFLFCGTAFIRYMKPASGYSDTMDKLVSIQYSILTPMLNPIIYSLKNKDVKNALKKLFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2C9</accession>
    <name>A0A6I9Y2C9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenase/reductase SDR family member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DHRS1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910960.1">
        <property type="nucleotide sequence ID" value="XM_014055485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2C9"/>
    <dbReference type="GeneID" id="106540394"/>
    <dbReference type="KEGG" id="tsr:106540394"/>
    <dbReference type="CTD" id="115817"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44147">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44147:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013910960.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="36134" checksum="BC8F71A42C1F2408" modified="2020-10-07" version="1">MASGTKPLLGHVCVVTGASRGIGKGVALQLSEAGATVYITARNRETLEQAAAEVQSRGGKCVPVVCDSTREEDVAALFQRVRQEQAGRLDVLVNSAFSAVTTLSKEIGVPFWETEPSLWDTVNNAGLRGNYFCAVHAARLMVPAGRGLIVIISSPGGLRYMFDVPYGVGKAACDRLAADCGVELRPFGVACVSLWPGLVRTELVVEQAEDVKKLFKNLSERLANKAESPEVSGKCVVALASDPHVMRHSGKVVLSPDLARLYRFKDVDGRDVYNYISVREILTELMPKLSSLFWFIPRFITFPKWALALYSSKFAIYPPIQPADFKPVKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2G4</accession>
    <name>A0A6I9Y2G4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibronectin type III domain-containing protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FNDC7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917253.1">
        <property type="nucleotide sequence ID" value="XM_014061778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2G4"/>
    <dbReference type="GeneID" id="106545271"/>
    <dbReference type="KEGG" id="tsr:106545271"/>
    <dbReference type="CTD" id="163479"/>
    <dbReference type="OrthoDB" id="9927686at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47135">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47135:SF1">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="12"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013917253.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="23323" checksum="DEAB228EC390030D" modified="2020-10-07" version="1">MFYVPPLANPCCPAYLSVTQVTQSTANISWSPGTGAKAYITILESDQRQARCRTLQTSCLLGCITCGTNYTVTVQAVSETGLISDCTYKGFSSSPCCPSGVKLYRLGNNSIRVYWRTSKESINYSTNLYGSKRNFTCISVGALTYCDITEIPCGDVYMVEVSPLIDHNRAKIAFCPKKMYSVTCSGNSVGMVIYRGRSADQHQVLSEKTETIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3Y9</accession>
    <name>A0A6I9Y3Y9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">H/ACA ribonucleoprotein complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nucleolar protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein family A member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">snoRNP protein NOP10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NOP10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918348.1">
        <property type="nucleotide sequence ID" value="XM_014062873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3Y9"/>
    <dbReference type="GeneID" id="106546095"/>
    <dbReference type="KEGG" id="tsr:106546095"/>
    <dbReference type="CTD" id="55505"/>
    <dbReference type="OrthoDB" id="13807at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904874">
        <property type="term" value="P:positive regulation of telomerase RNA localization to Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.40:FF:000002">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013918348.1"/>
        </source>
    </evidence>
    <sequence length="64" mass="7544" checksum="2301C01705CA0BAA" modified="2020-10-07" version="1">MFLQYYINEQGERVYTLKKMDPSGQPTCSAHPARFSPDDKYSRHRITIKKRFGVLMTQQPRPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4C3</accession>
    <name>A0A6I9Y4C3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC-type glutathione-S-conjugate transporter</fullName>
            <ecNumber evidence="12">7.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ABCC3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.3" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013912210.1">
        <property type="nucleotide sequence ID" value="XM_014056735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4C3"/>
    <dbReference type="GeneID" id="106541335"/>
    <dbReference type="KEGG" id="tsr:106541335"/>
    <dbReference type="CTD" id="8714"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000293">
        <property type="entry name" value="ATP binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005292">
        <property type="entry name" value="MRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00957">
        <property type="entry name" value="MRP_assoc_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF405">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="527"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="616"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="716"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="149"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="487"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="806"/>
            <end position="1038"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013912210.1"/>
        </source>
    </evidence>
    <sequence length="1042" mass="115759" checksum="9091876EB204241A" modified="2020-10-07" version="1">MKYKDARIKLMNEILSGIKVLKLYAWEPSFAEKVLEIRKNELRVLKKSAYLNSISTFAWISAPFLVALTTFAVYVSVDEKNVLDAEKAFVSLSLFNLLRFPLNMLPQVISNIVQTNVSLQRIQHFLSHDELDPNCVETKSITPGYAVTIRHGTFSWAKDLDPALKDINWLVPTGSLVAVVGQVGCGKSSLVSALLGEMEKLSGDVAVKGSVAYVPQLPWIQNATLKDNILFGHPLNELKYQTVLEGCALKQDLEMLPGGDQTEIGEKGINLSGGQRQRVSLARAVFSNTDVYLLDDPLSAVDSHVAKHIFDNVVGPEGILKGKTRILVTHGVHFLPQVDYIVVVVDGRISEMGSYQELLQQNGAFAEFLRNYAPDEDIEEDEPTIMEDEEVLLAEDTLSNHTDLADNEPVTNEVRKQFLRQLSVISSDGELSSKKSTKRKVCDKKSMEPPVKGTSEKLIQAETAETGTVKFTVFWQYMKSVGPLISLLICFLYSCQNGAAVGANVWLSAWTNDKVINGTQENVKMRIGVYAALGILQGLFVLISSFSLAMGGIKAARILHAALLENKLHTPQSFYDTTPTGRIINRFSKDIYVIDEVIPPTILMFLGTFFNSVSTMIVIIASTPIFAVVIIPLAVVYYFAQRFYVATSRQLKRLESVSRSPIYSNFSETVTGASIIRAYKRETSFIYTNDTKVNDNQKSYYPGIVANRWLGVRVEFVGNCVVFFAALFAVLSKNSLSAGLVGLSVSYALQVTMSLNWMVRMTSDLESNIVAVERVKEYSENETEAPWIIEDKRPPEEWPTQGEVVFTKYSVRYRKGLDLVLKDLSLSVKGGEKVGIVGRTGAGKSSMTLCLFRILEATKGEITIDGVKIADIGLHDLRSKLTIIPQDPVLFSGTLRMNLDPFNKYSEEEIWKALELSHLKRFVSSQPAMLEFECSEGGENLSVGQRQLVCLARALLRKTRILILDEATAAIDLETDDLIQMTIRTQFEDCTVLTIAHRLNTIMDYTRVLVLDRGRIAEFDSPSSLIASRGIFYGMAKDAGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5S0</accession>
    <name>A0A6I9Y5S0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase RNF182</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RING finger protein 182</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase RNF182</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106542230</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Interacts with ATP6V0C.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913405.1">
        <property type="nucleotide sequence ID" value="XM_014057930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5S0"/>
    <dbReference type="GeneID" id="106542230"/>
    <dbReference type="KEGG" id="tsr:106542230"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042285">
        <property type="entry name" value="RNF182"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46675">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46675:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="19"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013913405.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="27117" checksum="292075F32F544514" modified="2020-10-07" version="1">MNYPQTDREEKVLNEELECKICYQKFNLQSRKPKLLDCLHRVCAKCLTKILQVGSGSLHISCPFCRHETELSEEEIEGLPDDLNILSRLVTKDKSTWNSECKEVLLTPKTLASSSPSRGSSNCLVITIMEVQRDSPRTPSQNTLSDYYTDHSLDSESVNSTNQLDQDVFSKLCNHVPRILVWLLGFFYFGSLPLGIYLLVIQKVTLGIVCVSLVPSSLTVCLVYGFCQCLCQGMCDCSSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y607</accession>
    <name>A0A6I9Y607_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cyclin-dependent kinase inhibitor 1C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106542415</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913610.1">
        <property type="nucleotide sequence ID" value="XM_014058135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y607"/>
    <dbReference type="GeneID" id="106542415"/>
    <dbReference type="KEGG" id="tsr:106542415"/>
    <dbReference type="OrthoDB" id="6373236at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265:SF44">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 9" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="7">
        <location>
            <begin position="36"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="110"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013913610.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="26929" checksum="EB578DFF31B900A0" modified="2020-10-07" version="1">MKTKLRELGSSVSDARKRLLKYNIYLISRIVNSKRNLFGPVDHDELNRELKSKLREICEEGQKRWGYDFQTETPLAGSGRLQWEEVDGESVPAFYRETLQVGRCRFPTLMVRSTAPPGGEEASLEPDPRDPHQELGLSSIPSPLSPHDNTAALEGTTSAGEERLSQEDKADQRHSYSGIIIKPIPCVAAALKRASSTGVAHITDFFAKRKRVVDRVAGDHSSPLATSVTAVPNEQTPRKRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6I1</accession>
    <name>A0A6I9Y6I1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Oligodendrocyte transcription factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">OLIG3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914050.1">
        <property type="nucleotide sequence ID" value="XM_014058575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6I1"/>
    <dbReference type="GeneID" id="106542758"/>
    <dbReference type="KEGG" id="tsr:106542758"/>
    <dbReference type="CTD" id="167826"/>
    <dbReference type="OrthoDB" id="10011855at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18941">
        <property type="entry name" value="bHLH_TS_OLIG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000031">
        <property type="entry name" value="Oligodendrocyte transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032659">
        <property type="entry name" value="Olig3_bHLH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF96">
        <property type="entry name" value="OLIGODENDROCYTE TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="80"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013914050.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="28501" checksum="55341B01929D6038" modified="2020-10-07" version="1">MNSDSSSVSSRASSPDMEEVYLRDHHHHHHHHHHSVSSTQNEVGQKGSGESLSRSGAKGSAGESSKYKIKKQLSEQDLQQLRLKINGRERKRMHDLNLAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTSSLEEMKRLVGEIYGGHHAAFHCGTVAHAAGHPAHAAGAVHPILGAALAPPSSASLPGLGALRPPHSLLKTPPAPPALQLTGGGGGGGGGGGFQHWAGLPCPCSICQVPPPPHLASLPTPTAGLARLSTEAKDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y878</accession>
    <name>A0A6I9Y878_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106544641</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106544641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916450.1">
        <property type="nucleotide sequence ID" value="XM_014060975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y878"/>
    <dbReference type="GeneID" id="106544641"/>
    <dbReference type="KEGG" id="tsr:106544641"/>
    <dbReference type="OrthoDB" id="2384350at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042334">
        <property type="entry name" value="ANKRD31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040843">
        <property type="entry name" value="RAMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24176:SF14">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24176">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18755">
        <property type="entry name" value="RAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAMA" evidence="2">
        <location>
            <begin position="212"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916450.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="41823" checksum="37C7161B8CB6493F" modified="2020-10-07" version="1">MGHENICGMENSSLNQDHPNRIQDTSGENGEINSQKKDGPQLLVFENNVQKYNTAEVINSESSDATNSAPFLLNSVMCSTQQLKEIDCILVIPQETQNLSATSRLQILDNSTSVGDNICQPSTDTQNVCMNMLLSQNSNMNNASSIQPIVELESTCRFVSTDQDNMAPRKRILLNVNLESPGQFSHVPQNMISQNTSQYLKNQDYERDLYFKKNRKKKVQLLDLLELGKIKPGDDVLEFTLQDSKHKASLLGNGKVQTGSNSVYQNPVQWIKAILGNDICVSWKYVYNKVTYCGRPLSTIVNEMNDPKEPEVLLQQKNLLDSSYQTNSSKTPCFLQFNKIMLITDEEFLPWHAGEQYWDFYVKCENFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8B1</accession>
    <name>A0A6I9Y8B1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106544762</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013916615.1">
        <property type="nucleotide sequence ID" value="XM_014061140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8B1"/>
    <dbReference type="GeneID" id="106544762"/>
    <dbReference type="KEGG" id="tsr:106544762"/>
    <dbReference type="OrthoDB" id="8957712at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CNH_dom"/>
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    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
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    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="181"/>
            <end position="468"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="29"/>
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            <dbReference type="ARBA" id="ARBA00008874"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50219"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916615.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="55649" checksum="F8D477DE737CBF54" modified="2020-10-07" version="1">MPANNESYNLGMVSSHGGLEPPHPDKFSSISREGTLMIRETSGDHKRSGHPASNGFAGHVNLPDLVQQSHSPAGTPTEGLGRVSTHGQEMEPVAEYGMGSSTKASFAPFVDTRVYQTSPTDEDEDDDEESSATALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y901</accession>
    <name>A0A6I9Y901_9SAUR</name>
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        <submittedName>
            <fullName evidence="8">Tripartite motif-containing protein 12A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106545697</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
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            <taxon>Lepidosauria</taxon>
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            <taxon>Bifurcata</taxon>
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            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
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        <property type="nucleotide sequence ID" value="XM_014062340.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
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            <begin position="90"/>
            <end position="131"/>
        </location>
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            <begin position="230"/>
            <end position="271"/>
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        </location>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50089"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50119"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917815.1"/>
        </source>
    </evidence>
    <sequence length="279" mass="31590" checksum="220C1C7A5DCAC85D" modified="2020-10-07" version="1">MATGNIGKKLQSEITCLVCLEYLVQPVTLDCGHNFCHACIVKYWGQIPDQTACPQCRVAVPRWKFKPNGQLATVVQLIRQLSDQVKQMTRTSKTCQGHEPAMLVFCRDDLVPICLLCDGSQEHQTHDVIPVAQAAEEYKNVGGVLERYTKKKKFENPVSFPPEIKHEIWELQDFSAFLPGAVKQFEDCIMKCWKESSSELACPQCKHIGQNKPLENVVGLLKQLRAQAEGDTKCCQKHQERLELFCKNDQVFVCSVCRGSEEHRDHTVIPIETAGLEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9G8</accession>
    <name>A0A6I9Y9G8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein furry homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FRY</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918650.1">
        <property type="nucleotide sequence ID" value="XM_014063175.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tsr:106546330"/>
    <dbReference type="CTD" id="10129"/>
    <dbReference type="OrthoDB" id="6287725at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045842">
        <property type="entry name" value="Fry_C"/>
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        <property type="entry name" value="Furry/Tao3/Mor2"/>
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    <dbReference type="InterPro" id="IPR029473">
        <property type="entry name" value="MOR2-PAG1_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF29">
        <property type="entry name" value="PROTEIN FURRY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19421">
        <property type="entry name" value="Fry_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14228">
        <property type="entry name" value="MOR2-PAG1_mid"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="2">
        <location>
            <begin position="208"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1251"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1456"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1679"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1829"/>
            <end position="1940"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="3">
        <location>
            <begin position="2081"/>
            <end position="2333"/>
        </location>
    </feature>
    <feature type="domain" description="Protein furry C-terminal" evidence="5">
        <location>
            <begin position="2358"/>
            <end position="2914"/>
        </location>
    </feature>
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        <location>
            <begin position="1793"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1993"/>
            <end position="2020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2050"/>
            <end position="2071"/>
        </location>
    </feature>
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            <end position="2415"/>
        </location>
    </feature>
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        <location>
            <begin position="2551"/>
            <end position="2572"/>
        </location>
    </feature>
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        <location>
            <begin position="2600"/>
            <end position="2668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1993"/>
            <end position="2012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2053"/>
            <end position="2069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2404"/>
            <end position="2415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2560"/>
            <end position="2570"/>
        </location>
    </feature>
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        <location>
            <begin position="2612"/>
            <end position="2631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2639"/>
            <end position="2652"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF14222"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF14225"/>
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        <source>
            <dbReference type="Pfam" id="PF14228"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF19421"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013918650.1"/>
        </source>
    </evidence>
    <sequence length="2923" mass="330136" checksum="DDEBAA02F6D6C045" modified="2020-10-07" version="1">MAIGDSDLHFRTKCQLTSYQADYKLRRSSLPSELLRNDIKGKYQRNIRNLEPIHPRRPKKRPSLFPASPVGNGYIKPPVPSVSGTQKEKGPPTMLPINVDPDSKPGEYVLKSLFVNFTTLAERKIRIIMAEPLEKPLSKSLQHGEDPQFDQVISSMSSLSEYCLPSILRTLFDWYKRQNGIEDESHEYRPRTSTKSKSDEQQRDYLMERRDLAIDFIFSLVLIEVLKQIQLHPVIDGLVHDVINLAFKHFKYKEGYLGPNTGNMHIVADLYAEVIGVLAQAKFPAVKKKFMAELKELRHKEQNPYMVQSIISLIMGMKFFRIKMYPVEDFEASLQFMQECAHYFLEVKDKDIKHALAGLFVEILVPVAAAVKNEVNVPCLRNFVESLYDTTLELSSRKKHSLALYPLVTCLLCVSQKQFFLNRWHIFLNNCLSNLKNKDPKMARVALESLYRLLWVYMIRIKCESNTTTQSRLITIITTLFPKGSRGVVPRDMPLNIFVKIIQFIAQERLDFAMKEIIFDFLCVGKPAKAFSLNPERMNIGLRAFLVIADSLQQKDGEPPMPVTGAVLPSGNTLRVKKTYLSKTLTEEEAKMIGMSMYYSQVRKAVDNILRHLDKEVGRCMMLTNIQMLNKEPEDMITGERKPKIDLFRTCVAAIPRLLPDGMSKLELIDLLSRLSIHMDDELRHIAQNSLQGLLVDFVDWREDVLFGFTNFLLREVNDVHNSLLDTSLKLLLQLLTQWKLTIQTSGKTNEQTKVRSSELIANGSGQRTQTERSSYSNVLHAVEGFALVLLCSFQVATRKLAVLILREVRALFHALGQAEDDDRPMIDVMDQLSSSILESFIHVAVSDSTTLPLTYNVDLQWLVEWNAVLVNSHYDVKSPSHVWIFAQSVKDPWVLCLFSFLRQENLPKHCPTALNYAWPYAFTRLQLVMPLVDPNSPVNAKKTSTPSTGDHYVTLWRNYLILCFGVAKPSIMSPGHLRVSTPEIMATTPDGTISYDNKAIGTPSVGVLLKQLVPLMRLESIEITESLVLGFGRTNALVFRELVEELHPLMKEALERRPENKKRRERRDLLRLQLLRIFELLADAGVISDSTNGALERDTLALGALFLEYVDLTRMLLEAENDKEVEILKDMRAHFSAMLANLIQCVPVHHRRFLFPQQSLRHHLFILFSQWAGPFSIMFTPLDRYSDRNHQITRYQYCALKAMSAVLCCGPVFDNVGLSPDGYLYKWLDNILACQDLRVHQLGCEVVVLLLELNPDQINLFNWAIDRCYTGSYQLASGCFKAIATVCGSRNYPFDIVTLLNLVLFKASDTSREIYEISMQLMQILETKLFIYSKKIAERRPGSILYGTHGPLPPLYSVSLAHLSYELARMYPELTLPLFSEVSQRFPTTHPNGRQIMLTYLLPWLHNIELVDSRLLLPGSSPTTPEDEIKDKDGEVTVSCGLKGNGWGSPEATSLVLNNLMYMTAKYGDEVPGPEMENVWNALANNEKWSNNLRITLQFLISLCGVSSDTILLPYIKKVAIYLCRNNTIQTMEELLFELQQTDPVNPIVQHCDNPPFYRFTASNKASVVVSGTTSSSNTVVAGQDSFPDIEDNRILKETDERFSNVIRAHNRLESRYSNSSGGSYDDDKNDPISPYTSWLLNIVEMKQPQPLPMPFNGGCWAPLVDYLPETITPRGPLHRCNIAVIFMTEMVVDHSVREDWALHLPLLLHALFLGLDHYRPEVFEHSRKLLLHLLIVLSCNSNFQAIASMLLQTREMNETKTLTVQPVHLPEFFYTGGFDFLREYQSSPVPDSGLSSSSTSSSISLGGSSGNLPQITQEIEDIDPTVETNEKANKLIEFLTTRAFGPLWCHEDITPKNQNTKSAEQLTNFLHHVVSVFKESKSGFHLEQQLSEIALQTALSSSSRHYAGRSFQIFRALKQPLSAHALSDLLSRLVEVIGEHGEEIQGYVMEALLTLEATVDTLYDCLKNSDLLSCSSSPDLTSSAKLTANRKSTGQLNVNSGAASGSTGTAERSRHQRSFSVPKKFGVVDRTSDPPRSATLDRIQACTQQGLSSKTRSSSSLKDSLSDPTNINNPTNLLATIFWGAVALMESDFEFEYLMALRLLSKLLVHLPLDKVENREKLEKLQGQLKWTDFSGLQQLLLKGFTSLTTTDLTLQLFSLLTPVSRVSMVDSSQAIGFPLNVLCLLPHLIQHFDGPNQLCKDIAERIAQVCLEEKNPKLSNLAHVMTLYKTHSYTRDCATWVNVVCRYLHEAFADITLNMITYLAELLEKGLPNMQQSLLQIIYSLLSYMDLSNLPVKQFNMEVLKTIEKYVQSIHWREALNILKLVVARSASLVLPSYQHSDISKMEIHCIWNNASKELPGKTLEFQFDISETPIIGKRYDELQTSSGRDGKHRIMPVTRSTSSTSSGSNSNVLVPVSWKRPQYSQKKTKEKLVHVLSLCGQEVGLSKNPSVIFSSCGDLDLIEHQNSLVASEDGQREQENMDDSNSEQQFRVFRDFDFLDVELEDGEELQGESMDNFNWGVRRRSLDSLDKCDMQLLEESQLSGSIPSLNKINHEDSDESSEEEDLTTSQILEQSELIMTLSPSEDTNHIDALSTPCEAAASGPPPFSSRTSSFNTSLPEVNNLQVSEDSKVEGAQEEEDTSVQEDDLSGSTNELPPAFEYSDSFSLDMTEAEEKSDRLLDQYALASFGEGDRTASPQLSPFFSAILAAFQPTACDAAEEAWRSHINQLMCDSDGSCAVYTFHVFSSLFKNIQKRFCFLTCDAASYLGDNLRGIGSKFVSSSQMLTSCSECPTLFVDAETLLSCGLLEKLKFSVLELQEYLDTYNNRKEATLSWLANCKATFSGGSRDGVITCQPGDSEEKQMESLAQLELCQRLYKLHFQLLLLFQSYCKLIGQVHEVSTMPEVCNKKRKKQKNLFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9R2</accession>
    <name>A0A6I9Y9R2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DALR anticodon-binding domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DALRD3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919120.1">
        <property type="nucleotide sequence ID" value="XM_014063645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9R2"/>
    <dbReference type="GeneID" id="106546703"/>
    <dbReference type="KEGG" id="tsr:106546703"/>
    <dbReference type="CTD" id="55152"/>
    <dbReference type="OrthoDB" id="9990834at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106217">
        <property type="term" value="P:tRNA C3-cytosine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037380">
        <property type="entry name" value="DALRD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16043:SF1">
        <property type="entry name" value="DALR ANTICODON-BINDING DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16043">
        <property type="entry name" value="DALRD3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="1">
        <location>
            <begin position="405"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919120.1"/>
        </source>
    </evidence>
    <sequence length="545" mass="61178" checksum="21D1EAE1E228C2D3" modified="2020-10-07" version="1">METADSRLRVSETLAAFNAVLSGKAGDGARASLWFKESSARNLRSRDFLAPKAALRGAFANGQVPKDIVESVLSLKCPGVPPIHSCQNVPAGLIVQLDRSRVFEQILTALSVYTKAPLTSQGHCIILNCVPLHGRKDVKSLSLGHLRAILITDHLAGLLQAQGIKVQLVPAFVDKEIHQFLHQIQVDWPLGSEASRVPENILALKETLCQCQYTTFIQADQDEIVCKLHLKEFLEKQEGLEGYDPNLDVFLVTEEKLHHVAELQRVAQQCTEAVPQVCCSIVHIVNNEEAFQQQKVDLLWRLVAQPQSFTTHQKHLVCGPVKITCTDSCMNAFQYYQLRHSQMYKASVLKYGDLSKDASWTKVISVLTSAAIRFEMLSIVHQNQVFLDVEESNISMKGTKGGAFVMYNCARLATLFETYQRSVEQGLYPDFPQSSELNYSSLREEGEWLLLFNSILPFQEVLTLVTQLLLPSGGLRITVNTEAICKFLIQLSMDFSSYYNRVHILGEPRPHLFSQMFARLQLMRAVREVFHSALATFHLPPLSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAN5</accession>
    <name>A0A6I9YAN5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Acetoacetyl-CoA synthetase</fullName>
            <ecNumber evidence="2 8">6.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106547975</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Converts acetoacetate to acetoacetyl-CoA in the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>acetoacetate + ATP + CoA = acetoacetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16117"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.16" evidence="2 8"/>
    <dbReference type="RefSeq" id="XP_013920735.1">
        <property type="nucleotide sequence ID" value="XM_014065260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAN5"/>
    <dbReference type="GeneID" id="106547975"/>
    <dbReference type="KEGG" id="tsr:106547975"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030729">
        <property type="term" value="F:acetoacetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032024">
        <property type="term" value="P:positive regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005914">
        <property type="entry name" value="Acac_CoA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01217">
        <property type="entry name" value="ac_ac_CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42921">
        <property type="entry name" value="ACETOACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42921:SF1">
        <property type="entry name" value="ACETOACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="10">
        <location>
            <begin position="42"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="105"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013920735.1"/>
        </source>
    </evidence>
    <sequence length="531" mass="59507" checksum="82EDFBAC2EE5CEE7" modified="2020-10-07" version="1">MSGELEIMEAQRMWEPDSKRNTSMDRFRAAVTAAYGIRLANYNEFYQWSVQHYSDFWAEFWKFSGIAFSRLYDEVIDPSKSIADVPEWFKGSRLNYAENLLKHEENDKIALYAAREGKEEIEKVTFEDLRHRVAFYAAAMRKLGVQPGDRVVGYLPNGIHAVEAMLAAASIGAIWSATSPDFGINGVLDRFSQIQPKLIFSVEAVIYNGKRHNHLDKLQSVVKGLPDLKKVVVIPYVSSRESIDISRIPNSVFLEDFLGMGKGDQAPQLEFEQLPFSHPLFIMYSSGTTGAPKCMVHSAGGTLIQHLKEHILHGNMGCNDVIIYYTTTGWMMWNWLVSALAVGASVVLYDGSPLVPSPNVLWDLVDRLGITILGTGAKWLAVLEEKNLKPCETHNLQTLHTILSTGSPLKPQSYEYVYKHIKSSVLLGSISGGTDIVSCFMGHNVTIPVYKGEIQARNLAMAVEAWNEQGEPVWGESGELVCTKPMPCQPTHFWNDENGNKYRKAYFSRFSGMCRESRNLQAEIPRGAFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB22</accession>
    <name>A0A6I9YB22_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">B3GNTL1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921309.1">
        <property type="nucleotide sequence ID" value="XM_014065834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB22"/>
    <dbReference type="GeneID" id="106548458"/>
    <dbReference type="KEGG" id="tsr:106548458"/>
    <dbReference type="CTD" id="210004"/>
    <dbReference type="OrthoDB" id="206708at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06913">
        <property type="entry name" value="beta3GnTL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="67"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013921309.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="46522" checksum="B1DFFE9BBF3E77F2" modified="2020-10-07" version="1">MDVSETVCQEAGKSPPLLPDDVCANDPGLVKHDHSHSSSSPFFGNTELQPVAPFNRKAGGECRIQVSVIMPVYNAESWLDECLKSVLDQDYQGSIELSVFNDSSTDNSINIIKKWQILLEEAGIQVILGENYSTHPGGVGFAKNHAIDQSSGEYLCFLDSDDVMMPQRIRLQREANIKYPRSIIGCQIKREPAEATERYTRWINNLTHDQLLTQVFTSHGPTVIMPTWFCSREWFYLVGRFNEEGKGTPEDLLFFYEHLRKGGEVFRVDECLLLYRYHAQAATNTVLEETIWTHRVLFLEERILSNWTSFTIWNAGKQGRKLFRSLSPANQNKVTAFCDVDKNKITKGFYTYEESKETSKPRIPILHFKEASPPLIICVKLDLTDGQFEENLSLLNLKEGVDYYHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE71</accession>
    <name>A0A6I9YE71_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106549263</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922339.1">
        <property type="nucleotide sequence ID" value="XM_014066864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE71"/>
    <dbReference type="GeneID" id="106549263"/>
    <dbReference type="KEGG" id="tsr:106549263"/>
    <dbReference type="OrthoDB" id="9445802at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013922339.1"/>
        </source>
    </evidence>
    <sequence length="309" mass="35015" checksum="AA2D6D6B5012B1D4" modified="2020-10-07" version="1">MEVENLTSIREFLLLGLSAHHKIQMILFSTFLLIYSLTLLGNLAVITLVQLNPNLHTPMYFFLSHFSFLEICYTTSTMPQMLAHLLSGNGTISFIRCAIQGYISTTFGGTECFLLAVMAYDRYLAICHPLTYAITMDKRHRWMLTTVCWAICFLTSAINIAVTFQHTFCGSNHINHFFCELPLVVKLACGDTNITEIILFWTSVLVIVSPLIVVLTSYARILIMVFQMRSTASWHKAFSTCASHLAVVTLFYGTLISMYLKQKSESSSNLNKQIAVFYTVVTPLINPIIYTLRNKDIHKAVAKILKRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFA7</accession>
    <name>A0A6I9YFA7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CNOT11</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922913.1">
        <property type="nucleotide sequence ID" value="XM_014067438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFA7"/>
    <dbReference type="GeneID" id="106549724"/>
    <dbReference type="KEGG" id="tsr:106549724"/>
    <dbReference type="CTD" id="55571"/>
    <dbReference type="OrthoDB" id="10265389at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019312">
        <property type="entry name" value="CNOT11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10155">
        <property type="entry name" value="CNOT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="245"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013922913.1"/>
        </source>
    </evidence>
    <sequence length="526" mass="56905" checksum="BB760690150F4802" modified="2020-10-07" version="1">MLASGVRLSPEPGRAVEAAAGSIPGGGAVAASVAGFSTNSGSGGGSSSSSNNNSETIASLPGKMSLSPKELTSLLSIVSEEAGGSTFEGLSTAFHHYFGKAEHFRVGSVLVLLLQQPDLLPTPPQRLTALYLLWEMYRTEPLAANPFAAIFAHLLNPAPPERAPAGSGPGAAGSGGVGDDNERPPLSGFLPAITPPERFFLSQLMLAPPRELFKKTPRQIVSMDVGNMGQSVDISGLQLALAERQSELPTQSKASFPSILSDPDPDSSNSGFDSSVASQIIEALVSGPKPPIESHFRPEFIRPPPPLHICEDELAWLNPIEPDHTIQWDKSMCVKNSAGVEIKRIMAKAFKSPLSSQQQTQLLGELEKDPKLVYHIGLTPAKLPDLVENNPLVAIEMLLKLMQSSQITEYFSVLVNMDMSLHSMEVVNRLTTAVDLPPEFIHLYISNCISTCEQIKDKYMQNRLVRLVCVFLQSLIRNKIINVQDLFIEVQAFCIEFSRIREAAGLFRLLKTLDTGENPSETKTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFS2</accession>
    <name>A0A6I9YFS2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106549599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922770.1">
        <property type="nucleotide sequence ID" value="XM_014067295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFS2"/>
    <dbReference type="GeneID" id="106549599"/>
    <dbReference type="KEGG" id="tsr:106549599"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031697">
        <property type="term" value="F:beta-1 adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070699">
        <property type="term" value="F:type II activin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06734">
        <property type="entry name" value="PDZ4_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000113">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000150">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF27">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="203"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013922770.1"/>
        </source>
    </evidence>
    <sequence length="549" mass="60687" checksum="5C8768A2721436C6" modified="2020-10-07" version="1">MITNPNPSFSFRWMMAVPHKIGRIIEGSPADRCAKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLRIIPQEEVNSPASAPSSEKQSPMAQQHSPGTQQSPVAQPAPPQPLQTQGHENSYRSEVKARQDVKPDIRQPPFTDYRQPPLDYRHPPVLDYQQSPPLDYRQPPLIDYRQHSPDTRQFPLSDYRPPQDFDYFTVDLEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGETTRDMTHTRAIELIKSGGRRVRLLLKRGTGQVPEYDEPNSWRAPAATSPGLSEITLSPDDIVLPLSSSHLAQPSDPSCQISPDSAWDIKREHHVNKPKEFPVNGHRKKRLGEQTERPASPKKVDRSKHEEVWRKTSSEGKSTESGRPLPETKAGGLNPMAEDGVRGHGCAGTNEEWVGRSKSKRGGSLGGKDPYVTVAAEKKSTEHVKLDSIGATKAYSSNSCSSHGSDVDHSLKEPERKPGEFPWKGHAHPIPTHNPNPTARKATVSPGPWKIPGSDKLPGILKSTPSAMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIH9</accession>
    <name>A0A6I9YIH9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Transthyretin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106550372</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the transthyretin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923749.1">
        <property type="nucleotide sequence ID" value="XM_014068274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIH9"/>
    <dbReference type="GeneID" id="106550372"/>
    <dbReference type="KEGG" id="tsr:106550372"/>
    <dbReference type="OrthoDB" id="10265230at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070324">
        <property type="term" value="F:thyroid hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05821">
        <property type="entry name" value="TLP_Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.180:FF:000002">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023418">
        <property type="entry name" value="Thyroxine_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000895">
        <property type="entry name" value="Transthyretin/HIU_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023419">
        <property type="entry name" value="Transthyretin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395:SF12">
        <property type="entry name" value="TRANSTHYRETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="URICASE AND TRANSTHYRETIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00189">
        <property type="entry name" value="TRNSTHYRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00095">
        <property type="entry name" value="TR_THY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00768">
        <property type="entry name" value="TRANSTHYRETIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00769">
        <property type="entry name" value="TRANSTHYRETIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0765">Sulfation</keyword>
    <keyword evidence="8 9" id="KW-0795">Thyroid hormone</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027153161" description="Transthyretin" evidence="9">
        <location>
            <begin position="21"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="10">
        <location>
            <begin position="46"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923749.1"/>
        </source>
    </evidence>
    <sequence length="166" mass="18480" checksum="61371A1B2416C195" modified="2020-10-07" version="1">MGPFSLFFFCLATVGYLIEAVPVAISYQNSNRNLFCCIKEPHSSADSKCPLMVKVLDAVRGTPATNLPVKLFKKGEDEAWKEFSSGKTNEFGEIHELITDELFTEGSYKLEFDTSSYWKALGISPFHEYAEVVFTANDSGHRHYTIAALLSPFSYSTTAVVSIPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIJ7</accession>
    <name>A0A6I9YIJ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Translocon-associated protein subunit alpha</fullName>
            <shortName evidence="12">TRAP-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Signal sequence receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SSR1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 12">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma. Interacts with palmitoylated calnexin (CALX), the interaction is required for efficient folding of glycosylated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TRAP-alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924037.1">
        <property type="nucleotide sequence ID" value="XM_014068562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIJ7"/>
    <dbReference type="GeneID" id="106550622"/>
    <dbReference type="KEGG" id="tsr:106550622"/>
    <dbReference type="CTD" id="6745"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF0">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027068433" description="Translocon-associated protein subunit alpha" evidence="14">
        <location>
            <begin position="19"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="31"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="31"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013924037.1"/>
        </source>
    </evidence>
    <sequence length="287" mass="32266" checksum="4C4B967185694633" modified="2020-10-07" version="1">PQLLVFVLLAFPAVLLRSGPGSGLLVAAQDATEDEEAIEDSIVEDEDDEAEVEEDEPTELTEEKEEDDLSGEPKASPNADTTILFVKGEDFPANNIVKFLVGFTNKGTEDFVVESLDASFRYPQDYQFFIQNFTALSLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDQNGNFFQDAVFNQTVTIIEREDGLDGETIFMYMFLSGLGLLVIVGLHQLLESRKRKRPAQKVEMGTSNQNDVDMSWIPQETLNQIMQSRRDKASPRRLPRKRTQKRSVGSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKT1</accession>
    <name>A0A6I9YKT1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein GUCD1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GUCD1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924842.1">
        <property type="nucleotide sequence ID" value="XM_014069367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKT1"/>
    <dbReference type="GeneID" id="106551290"/>
    <dbReference type="KEGG" id="tsr:106551290"/>
    <dbReference type="CTD" id="83606"/>
    <dbReference type="OrthoDB" id="206796at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018616">
        <property type="entry name" value="GUCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31400">
        <property type="entry name" value="GUANYLYL CYCLASE DOMAIN CONTAINING PROTEIN 1 GUCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31400:SF1">
        <property type="entry name" value="PROTEIN GUCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09778">
        <property type="entry name" value="Guanylate_cyc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924842.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="32472" checksum="810F62EEDBECFC4D" modified="2020-10-07" version="1">MKTPAEEVAALPPSGGSSGPGAPSCAAGRGAGELRRPLPSPALADAGAEGVGEAIQLKVPALQQLYHWDCGLACSRMALRYLNLLNDEDFQRAVRDLQLTKSIWTIDLAYLMRHFGVKHLFCTQTLGVDKGYKNQSFYRKHFDIEENRVNQLFAQAKACKVLVEKRTFLARGWQMATQRTTVTVTVGDIQEHLSQGHIAIVLVNAVLLLCDLCSSPVKYCCFLPIGQKCFCRSPDYQGHFIVLCGYNRALECIYYNNPAYADRTCSTSIHNFEEARTSYGTDEDILFIYQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YL98</accession>
    <name>A0A6I9YL98_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CUB and zona pellucida-like domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CUZD1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924809.1">
        <property type="nucleotide sequence ID" value="XM_014069334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YL98"/>
    <dbReference type="GeneID" id="106551272"/>
    <dbReference type="KEGG" id="tsr:106551272"/>
    <dbReference type="CTD" id="50624"/>
    <dbReference type="OrthoDB" id="10063988at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000005">
        <property type="entry name" value="Deleted in malignant brain tumors 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF27">
        <property type="entry name" value="CUB AND ZONA PELLUCIDA-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="72"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="200"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="8">
        <location>
            <begin position="321"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924809.1"/>
        </source>
    </evidence>
    <sequence length="649" mass="73482" checksum="2A96D95C6F18083C" modified="2020-10-07" version="1">MGYFMNLNQNAYIEATVYSFTRNQILFMDSCVASPNRYDFYTETYVMINRGFVNFNIHALQKATVVKGYDIVGTNFFFEKRNTTCVKTLIKSFQAIPIQLNANENCIWEIKGESDTNQTIRLIFSYIEFSPLSSCDEEYIEIYDGPSTNSSVIGKICRTKESAFHSSASALTFRISTASTNFTRNFHALYYYISPDTPRCGGYLTGPEGTFTSPNYPKKHQPMTYCVWHIETKPYTQINLIFKEILIEMDENCRFDFLAIFDGPTSDSPLIKKVCGNLKPTFKSSSNAMTIVMSTDYANSYRGFSARYTTTPISQPNTSLSCSSDRMTITLSDFYLASLNYNASSLYLNDQTCKPVSYNPVVFSFPINSCGTIKKTEGQSITYSNIVTALPAGVIITHQKKVEIVAKCIMENNSTVEVVYVTESEHLDNTTAVGRYNLSMSFYETDSFSKPIVETPYFVDLNQMLYAQISLHSSDPNLVVFVDTCTASPDLNSKSPKYDLVRTGCTKDDSFMAYPILEHYGRFRFRSFRFLKHYPSVYLHCEVLICDSKNSNSRCTKGCISRHKREISSYKWKGDAVIGPLRLKGEESSMDRSAISSEVYPEESLKAQPQILYILAFVVLAANTVLLVGLAAKRLIARETGYRYQKLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLE8</accession>
    <name>A0A6I9YLE8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106551383</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106551383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924961.1">
        <property type="nucleotide sequence ID" value="XM_014069486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLE8"/>
    <dbReference type="GeneID" id="106551383"/>
    <dbReference type="KEGG" id="tsr:106551383"/>
    <dbReference type="OrthoDB" id="76966at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040401">
        <property type="entry name" value="CCDC162"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33331:SF13">
        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING 162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33331">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924961.1"/>
        </source>
    </evidence>
    <sequence length="560" mass="64893" checksum="8A6020BE699F4251" modified="2020-10-07" version="1">MELSFLYLRHLRIRELQRICLGILNYFRSIERTLTISTSGLASCSGHLISTAEDASWVNAAKGGTGAFGGLSSHSYMHYTPADYKVQSVQFMEFAEVENHDDFYTVEEAYIHTQDQRGAFVMYDVALQDLKETEKQLLLVASQYIEVEKGQKAGPDSSSNRYSRRAETSVDRSAVLLDLWTCEADFLKDKWQLLDSYFEVYQHALDSEERFALAQVITNIMYRRPRFDFHLGYFVNTYRDECTCLKLHLCLVRDIMHRQIENQRKYNYQIWCGGPKGCISEFGFPTYIVTKQLIALSNSTTALKNMYLLEFHSSLGLVSLIPKALDHALQEFQQICRPNTASAAIHLEEQILLLSLDGWMTMENPESFYGAQIQRDIFADVLVENPLIIREIVTLALKSVSEEEQRPSKEKQTITLNVFSRLLELLTLRHRLIEAAVEGAQLGRLYKEFAKEMGFNEFHLYLRPVYFECATHKEKADQFSPIFIASLLEDQDLNLDRYIPSNLMLEIQDIDNQIGKFSFCTRESILQVTEIFFIPVSKLLYYFILLFRPLSYLFYFVLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLS9</accession>
    <name>A0A6I9YLS9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Axin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">39S ribosomal protein L28, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Axis inhibition protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Large ribosomal subunit protein bL28m</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">AXIN1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925187.1">
        <property type="nucleotide sequence ID" value="XM_014069712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLS9"/>
    <dbReference type="GeneID" id="106551582"/>
    <dbReference type="KEGG" id="tsr:106551582"/>
    <dbReference type="CTD" id="8312"/>
    <dbReference type="OrthoDB" id="10007451at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030877">
        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11582">
        <property type="entry name" value="Axin_TNKS_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08707">
        <property type="entry name" value="RGS_Axin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000003">
        <property type="entry name" value="Axin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.196.10:FF:000002">
        <property type="entry name" value="Axin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.130:FF:000002">
        <property type="entry name" value="Axin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043581">
        <property type="entry name" value="Axin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014936">
        <property type="entry name" value="Axin_b-cat-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032101">
        <property type="entry name" value="Axin_TNKS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001158">
        <property type="entry name" value="DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038207">
        <property type="entry name" value="DIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribosomal_bL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="Ribosomal_bL28/bL31-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46102">
        <property type="entry name" value="AXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46102:SF3">
        <property type="entry name" value="AXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16646">
        <property type="entry name" value="AXIN1_TNKS_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08833">
        <property type="entry name" value="Axin_b-cat_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00778">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00021">
        <property type="entry name" value="DAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="L28p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50841">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="13" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 20" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="RGS" evidence="22">
        <location>
            <begin position="378"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="DIX" evidence="23">
        <location>
            <begin position="1032"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="284"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="505"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="548"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="612"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="885"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="925"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1000"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="558"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="612"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="890"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1000"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013925187.1"/>
        </source>
    </evidence>
    <sequence length="1114" mass="124501" checksum="02CB582E57CB1AE1" modified="2020-10-07" version="1">MPLHKVPPRLWDSLRLQRGILARLPPHYLRALREDAAAPPPAVHWRPPAGEYARAPGPLEGVRQQVVPVPVYFPPESQEGLWGGEGCVAGYRYAHDDKLSRRLKKMWKPQVFNREFYSEILGKSLRIAVTMRTLEQVDKAFGFDFYILKTPKSELCSKLGMDLKRTLLLRLARRDPSLHPDDPAKREAVYNKYKEFVIPEEEAEWIGLSLEEAVEKQRVLEKKDPVPLFRVYAEELILHLQKQQLYLYKKTGDGNGGSLGRVISAREANICIVVVAPEAGALRPAPAREMSAPEKGFSLDLGGGGFTEDAPRPPVPGEEGDLLSADPRPAGHGFSAGKGDGGKGETTTATPRRPDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGIHLFRTFLKHESCADLLDFWFACSGFRKLAPCDSKEEKRLKLAKAIYKKYILDNNGIVSQQVKPATKSFIKDSVLKQLIDPAMFDQAQMEIQSMIEENTYPLFLKSDIYLEYTRTGGESPKVHSDQSSGSGTGKGLPGYLPTLNEDEEWKCDQDILEGEEGQVAADPESAPSSRFTQKLLLETTSQPAPRTRHYSEGREFRPGPWRETGHPYYVNTGYALAPATSANDSEQHSMSSDADTMSLTDSSLDGVPPYRLRKQHRREMQESAKANGRVPLPHIPRTYRVPKDIHVEPQKFAAELINRLKEVLRDREAEAKLEERLKRVRAEEEGEDADLALGHKLVPTQLLPHFAPRYADAHEENPESILDEHVQRVMKTPGCQSPKPRSPDSGLVGKLHSLAGSVPLGHSKHASKSGLKLEAPSLFHHKHVYHHMVHHHAGLKAKETAEEAASQRTQGPFAWTVDPHGCTAKSRAYGESMGLATGPLDAMAYSGKAGLLAKRGAKKPEPGKNESGSHDVPGSPEDLERNQKILQWIIEGEKEISRHRKTTHSSSGTKKQLVHEPPRPAPIDRPVAVHPWISFRNVVQPSHPFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRTGKPAAKQRVKGPKRLGSGFSQPCENIVVAYYFCGEPIPYRTLVKGRVVTLGQFKELLTKKGNYRYYFKKVSDEFDCGVVFEEVQEEDAVLPIFEEKIIGKVEKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPZ3</accession>
    <name>A0A6I9YPZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat and SOCS box protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">ASB8</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits IFN-beta production through the IRF3 signaling pathway by targeting TBK1 via 'Lys-48'-linked ubiquitination, leading to its proteasomal degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with TBK1; this interaction promotes TBK1 proteasomal degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925978.1">
        <property type="nucleotide sequence ID" value="XM_014070503.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925979.1">
        <property type="nucleotide sequence ID" value="XM_014070504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPZ3"/>
    <dbReference type="GeneID" id="106552276"/>
    <dbReference type="KEGG" id="tsr:106552276"/>
    <dbReference type="CTD" id="140461"/>
    <dbReference type="OrthoDB" id="10258888at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03727">
        <property type="entry name" value="SOCS_ASB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000274">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037332">
        <property type="entry name" value="ASB8_SOCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24134:SF9">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24134">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN DDB_G0279043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="13">
        <location>
            <begin position="231"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925979.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="31682" checksum="478990F5A404C0A6" modified="2020-10-07" version="1">MWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRRGADVNCMHGTLKPLHCACMVADADCIELLLEKGAQVNALDGYNRTALHYAAEKDETCVEILLEYGANPNALDGNKDTPLHWAAFKNNAECVRTLLESGAFVNALDYNNDTPLSWAAMKGNLECVSILLEYGAEVRIVNMKGQTPISRLVALLVRGLGTEREDSCFDLLHRAVGHFELRKNGAMPREVMRDQQLCEKLTMLCSVPGTLKTLSRYAVRHSLGMQFLPDAVKELPLPESLKEYVLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQG5</accession>
    <name>A0A6I9YQG5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pleckstrin homology-like domain family B member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106552894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926763.1">
        <property type="nucleotide sequence ID" value="XM_014071288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQG5"/>
    <dbReference type="GeneID" id="106552894"/>
    <dbReference type="KEGG" id="tsr:106552894"/>
    <dbReference type="OrthoDB" id="6020705at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052212">
        <property type="entry name" value="PH-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156:SF22">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN FAMILY B MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN, FAMILY B, MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="328"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926763.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="41233" checksum="E42EBCFEDED535DB" modified="2020-10-07" version="1">MDLEGALLEGELQEERQELRQEEERLLELKERLAETELRCREEREKEKARLQRERQRVEELQRQHAESQIHLNNQPESMRERMQKQLQETSEMLEGALRCYEDLEFQQLERESHLEEEKEAICHALTEEITQLQNSINQRKKTVQKLEGQALLTQEQMMGVCQRFAQEEGEAVSHLNAEKSRLMDMSQEYSANGEDLSENKEGVTQLTFTQKTDRKVIVLGSDQSDSSSCVFSVCSSVKGSIGLQRTASLPRRSRDRLPARAPQRPVSMHGALDPSLLVAQLSSGSNGHYLGSPNLQFSRYNKPLYQLLGGQATTRQVGGTSQGAYSLENCVTKLAEMERRVREAMAERERLLQERVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR73</accession>
    <name>A0A6I9YR73_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CUB domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CDCP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926429.1">
        <property type="nucleotide sequence ID" value="XM_014070954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR73"/>
    <dbReference type="GeneID" id="106552633"/>
    <dbReference type="KEGG" id="tsr:106552633"/>
    <dbReference type="CTD" id="64866"/>
    <dbReference type="OrthoDB" id="8960034at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038811">
        <property type="entry name" value="CDCP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056266">
        <property type="entry name" value="CDCP1_CUB_3rd_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056268">
        <property type="entry name" value="CUB_CDCP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056269">
        <property type="entry name" value="CUB_CDCP1_2nd_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056965">
        <property type="entry name" value="CUB_CDCP1_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14477">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14477:SF1">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23665">
        <property type="entry name" value="CDCP1_CUB_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23667">
        <property type="entry name" value="CUB_CDCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23668">
        <property type="entry name" value="CUB_CDCP1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25142">
        <property type="entry name" value="CUB_CDCP1_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 first CUB" evidence="4">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 second and fifth CUB" evidence="5">
        <location>
            <begin position="91"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 third and sixth CUB" evidence="3">
        <location>
            <begin position="200"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 fourth CUB" evidence="6">
        <location>
            <begin position="324"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 second and fifth CUB" evidence="5">
        <location>
            <begin position="410"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 third and sixth CUB" evidence="3">
        <location>
            <begin position="519"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="790"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="794"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926429.1"/>
        </source>
    </evidence>
    <sequence length="814" mass="91631" checksum="FFDE4F0D3F795981" modified="2020-10-07" version="1">MLAAVLALLKAGAFEISLPLGGNITVKIKPGFSFLNTCALREGKNPHFLEKIIEPGQKFQFTFDCSTPEKYWSILVEKNIDCLSGQCPFGDIQLQPPGLPHLNRTFIWEAKANRRVGLELKFSPWLRQILPGDTCPDQIIYNISSRLGKDRVQIGTFCRNGSVSRVKAQGDAILTLQLPWDSKLAASGVKLASRSSIQRLCIIESTFKGKSSATLMSANYPLGLPEDELMTWQFVLPSSLRADINFINYTKPNCERKYVRVEYSLPDYSPDTRVLSLDDVQPVNIPGSFNLSLYGCDQDDSSGLLRLLFKVIVNYPKNEENVTYKIDLTHEKTNLTIYSKSSMNECLICVHSSRNCRPNVTLEYGVTYFATFLCQNLDNIRIIAEQSIVCWNARTCENLFFQLAVPSVLLQLPILIETFTWKIQALEAVNIEIRSSTLKLKQHIPNRDQRCNGSYSYIINGSSPGRSWSLGYFCPVGDIEKIQIKDNVTLTLKTFGKRWFNESNTQKGDLKLFFKPVLQEDCIFTLSPDPKTKVYIQTPNWLEGLLPFMSVYWNFSVPPKQVAQLTFLNERMGITCEKSRAFIYVKEQSAKAVETVCRDDNVLPKTLNLRQDFWINITNCKPSAKQQLSLQFLVTFQKNSDVTVIIGVVAGGMAVLAIIGLLICCVKKKRKNKRKESHNPVMGVYNTNVNTLLPERQGIFKKERKKNDSHVYAVIEDVMVYGHLLDNSTRPEMADVGVYRPFSGPVSNIPPSPPPIRKISKSSDLQESSNVLVTNSENYTFAHHVPENIHNGDLNVSGNGHVGSRNNEQGNSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRB9</accession>
    <name>A0A6I9YRB9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Class E basic helix-loop-helix protein 41-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106552962</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926847.1">
        <property type="nucleotide sequence ID" value="XM_014071372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRB9"/>
    <dbReference type="GeneID" id="106552962"/>
    <dbReference type="KEGG" id="tsr:106552962"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Orange" evidence="6">
        <location>
            <begin position="37"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="90"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926847.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="24953" checksum="3C3FBBC1FCAB7BD4" modified="2020-10-07" version="1">MNCELYVLITPIVLGHAFFSGDRSLKSPPQSADLEAFHSGFQMCTKEVLRYLSPPERWTSREPRCAQLLGHLHSMCRRFLPGSPLFTPRAQGRCCSPQDGPQQLPPDQRPGGTLKQEPPQPNCVPVIQRTQNWGRGAATHQLPPSGPEQAGEPDTDTDSGYGGEGEGKKTPQGGLRVKREPADVELEVKKVKMAPAIMEAKTPSDQPFPTLLLWHSSNVRIVFVLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRI9</accession>
    <name>A0A6I9YRI9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">RNA-binding protein Raly isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">RALY</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM HNRPC family. RALY subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926250.1">
        <property type="nucleotide sequence ID" value="XM_014070775.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926251.1">
        <property type="nucleotide sequence ID" value="XM_014070776.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926252.1">
        <property type="nucleotide sequence ID" value="XM_014070777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRI9"/>
    <dbReference type="GeneID" id="106552483"/>
    <dbReference type="KEGG" id="tsr:106552483"/>
    <dbReference type="CTD" id="22913"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12604">
        <property type="entry name" value="RRM_RALY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000019">
        <property type="entry name" value="heterogeneous nuclear ribonucleoproteins C1/C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017347">
        <property type="entry name" value="hnRNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034502">
        <property type="entry name" value="RALY_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF6">
        <property type="entry name" value="RNA-BINDING PROTEIN RALY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037992">
        <property type="entry name" value="hnRNP-C_Raly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013926251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926252.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="32019" checksum="1B7803B87DED5F4C" modified="2020-10-07" version="1">MSLKVQTSNITNKNDPKSINSRVFIGNLNTAVVKKSDVETIFSKYGRVVGCSVHKGYAFVQYSNERHARAAVLGENGRVLAGQTLDINMAGEPKPNRPKGLKRTASALYSSGYDFDYDYYRDDFYDRLFEYRGRVSPIPRMVPVKRPRVTIPLVRRVKATIPVKLFARSAALVNSSAKLKLKCSELQTIKTELTQIKSNIDALLGRLEQIAEQQKAPTEVRKKADGNKSEISQEDTSSEAEANTEEPLNGEEEEEEEEEEGLVQDDCDDELENSHFTYVEDSSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRP9</accession>
    <name>A0A6I9YRP9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calmodulin-regulated spectrin-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CAMSAP2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926987.1">
        <property type="nucleotide sequence ID" value="XM_014071512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRP9"/>
    <dbReference type="GeneID" id="106553082"/>
    <dbReference type="KEGG" id="tsr:106553082"/>
    <dbReference type="CTD" id="23271"/>
    <dbReference type="OrthoDB" id="2125658at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000001">
        <property type="entry name" value="Calmodulin-regulated spectrin-associated protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031372">
        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF1">
        <property type="entry name" value="CALMODULIN-REGULATED SPECTRIN-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CKK" evidence="9">
        <location>
            <begin position="1066"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="369"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="531"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="652"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="753"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="848"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="916"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="973"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="477"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="399"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="531"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="672"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="688"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="756"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="850"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="973"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1005"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013926987.1"/>
        </source>
    </evidence>
    <sequence length="1206" mass="135434" checksum="DA6F4428A87DAA2C" modified="2020-10-07" version="1">MSLADSLYNLQLIQEFCQEYLNQCCHFALEDMLYAASSVKSNYLVFMAELFWWFEVVKPSFVQPRAVGHPQADPPMEASSVPPTNVNQRSYPGVLHDSDLITSVGTSTFIPSHQIPPPPRHTQQQPYSSMPGGIKRSTSMSYVDGYVGTWPKEKRSSVHGVSFDISFDKEDSLRLAPPNRGITRSISNEGLVQNANRLPRHVKRNISFKPLDDEEDSEDSEIEKDEELCSDLEAVSNQRNANQVNTYTLRNGGLQNRLVTTDEFGNQIETPSIEQALQIIHDSVESANVTPPAQFTNGFFLHNKEMSILNSNITPNQSGPDIIADTKDVLSPVTDNTEVDTGIHVPSDIPETLDEDSTLRDYTVSLDSDLEESSRFLQESDSRGINHKEQLSPCPSSISTKSQAGSSPSSSSGVRMTSFAEQKFKKMNPTDSRSSGSSSQKTTPEGSEMNIPHVVSWAQIPEEPPLPQGRDTTQLLASEMVQLKMKLEEKRRAIEAQKKKVEAAFTKQRQKMGRTAFLNVVKKKSDGISPLREEAAGAEGEKVFTDNSEAKERKNQKPGEELKKTSEVLKVSTETPSVTWLKPPNTPADAEKQSNLVSPSEENLNEGDLLEYTKSIEKLNSSLHFLQQEMQRLSLQQEMLLQMREQQAWVISPPQPSPQKQVRELKPPAKLSGSSSPVVPFSVETPRPSHQSPQLSSRKNSSLPCKIQRIPRPNELKITPLNRTLTAPRSVDSLPRLRRFSPSQVQTRSFVCFGDDGERKPTEVKNATEEASDKEEAGLKDAEKQGTGEKVEQKTEEEQMRPLESMVSEVLSQPVTEIVCLTPNEEPLNPPVLPPPKTVSLIEVSLTDLKPPEKLEDSIEKSDGESDKEQAEDDQKVCCGFFFKDDQKPENDMAVKRAALLEKRLRRERETLLRKQQLEAEMEHKKEETKRKSEEERQKKEDERARRDFIRQEYMRRKQLKLMEDMDIVLKHRSHFSKQKKARPKSIHRDHIESPKTPIKGPPVSSLSLASLNTADNESVQSGKRTPRSESVEGFLSPSCGSRNGEKDWENASTTSSVASATEYTGPKLYKEPSAKSNKHIIQNALAHCCLAGKVNEGQKNKILEEMEKSDANNFLILFRDSGCQFRSLYTYCPETEEISKLTGIGPRSITKRMIEALYKYNSDRKQFSHIPAKTLSASVDAITIHSHLWQSKRPVTPKKLSSSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRV0</accession>
    <name>A0A6I9YRV0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Neurotensin receptor type 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NTSR1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927263.1">
        <property type="nucleotide sequence ID" value="XM_014071788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRV0"/>
    <dbReference type="GeneID" id="106553313"/>
    <dbReference type="KEGG" id="tsr:106553313"/>
    <dbReference type="CTD" id="4923"/>
    <dbReference type="OrthoDB" id="9835116at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016492">
        <property type="term" value="F:G protein-coupled neurotensin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15355">
        <property type="entry name" value="7tmA_NTSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003985">
        <property type="entry name" value="NT1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003984">
        <property type="entry name" value="NT_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF9">
        <property type="entry name" value="NEUROTENSIN RECEPTOR TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01479">
        <property type="entry name" value="NEUROTENSINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01480">
        <property type="entry name" value="NEUROTENSN1R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 12 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="335"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="76"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="392"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013927263.1"/>
        </source>
    </evidence>
    <sequence length="412" mass="47329" checksum="AE267245CE2F1A41" modified="2020-10-07" version="1">MHLNTTSSFYPLSYLMSEFHLPSANSVLPEAENHSNCSGPGSIPHNEEDLDVNTDIYSKVMVTVIYLVLFLLGTVGNSITAYTLVRKKSLQNLQSTVHYHLASLAFSDLLIFLLCMPIELYNFIWVHHPWAFGNDVCKGYYFLRDACTYATSLNIASLSVERYMAICHPFKAKSIMSRSRTKKFISCIWVASFLLAIPMILIMGEIYRVPQDPDSLVCTQIVDDSVLKVAIQVNVFISFVFPMAVISVLNTIIANQLIIMFKQAAQEHQVCTIGGQQTMLSMSMEPGRVQTLKHGVRVLRAVVIAFVVCWLPYHVRRLMYCYISEQQWSDFLYDFYHYFYMLTNILFYVSSAINPVLYNLVSTNFRQIFFSTFISICLPWRKKKKRTKFNRKSNSISSNHTFSSQVTRETTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS59</accession>
    <name>A0A6I9YS59_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">T-cell surface glycoprotein CD5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CD5</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927122.1">
        <property type="nucleotide sequence ID" value="XM_014071647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS59"/>
    <dbReference type="GeneID" id="106553198"/>
    <dbReference type="KEGG" id="tsr:106553198"/>
    <dbReference type="CTD" id="921"/>
    <dbReference type="OrthoDB" id="544868at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003566">
        <property type="entry name" value="Tcell_CD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47309">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47309:SF1">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01409">
        <property type="entry name" value="TCELLCD5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="330"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013927122.1"/>
        </source>
    </evidence>
    <sequence length="356" mass="39872" checksum="BF40D734C44830BD" modified="2020-10-07" version="1">MDLRLNGAGCRCSGWLEVLHAATWKRIDSRNFTQQNAELACRQLGCHLPFGILQPYVEELEKHETCLVTCQGNETALKDCIACDSHDFFSLGVFLVCQQAPVQTTMVLQPTTAPVVTSSKPFGRKIQTFADGDNHPRTNIEEGTSLCSGIQEPDVKGYEKMLCLSLLRWWDKLVPRTCQGVNCEKPGHKDKNREKSLHHWEKLQCEKRNLSMGNCSASTQECLHLTLVRCSDQDLKSQKSSSETVLGILLGLVLIVGLLSICALPTYKKVMKKYSKKRQQQWIGPSGVNQNVSFHRKSSTAFQSHIENPVVQEERNKASKTYLSPYAAMERATNRSSSPLDNSSDSDYDLCSAQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTD2</accession>
    <name>A0A6I9YTD2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GRPEL1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19. Binds to HSP70, HSC70 and HSJ1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927224.1">
        <property type="nucleotide sequence ID" value="XM_014071749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTD2"/>
    <dbReference type="GeneID" id="106553281"/>
    <dbReference type="KEGG" id="tsr:106553281"/>
    <dbReference type="CTD" id="80273"/>
    <dbReference type="OrthoDB" id="201635at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.20.20:FF:000003">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF25">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013927224.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="24765" checksum="83D9D61647C6BE53" modified="2020-10-07" version="1">MAAVVVCGARRLAGRAAFLLSGASRRSTQLLCTVTQEKSTGPNLEEEQSQGHNEQKPDSCSDANILTEEKTKLEQQLKEITDKYKRALADAENLRQRTQKLVEEAKLYGIQSFCKDLLEVADVLEKATESVPKEELKAENPHLKNLYEGLAMTEVQIQKVFKKHGLVKLNPLGAKFDPYEHEALFQVPAEGKEPGTVALVSKVGYKLHSRTLRPALVAVVKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTK1</accession>
    <name>A0A6I9YTK1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 252-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106553836</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927888.1">
        <property type="nucleotide sequence ID" value="XM_014072413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTK1"/>
    <dbReference type="GeneID" id="106553836"/>
    <dbReference type="KEGG" id="tsr:106553836"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000029">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000638">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000098">
        <property type="entry name" value="Zinc finger protein 614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000361">
        <property type="entry name" value="Zinc finger protein 658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000495">
        <property type="entry name" value="zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000028">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="328"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="468"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="496"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="552"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927888.1"/>
        </source>
    </evidence>
    <sequence length="590" mass="67206" checksum="3F49F1A3FA1DDD79" modified="2020-10-07" version="1">MEASPCEEGKSSLDEIKPYLCPGCGKTFIRNAHLLKHLMIHHEGEKLYKCQYCEKSFSQNYHLVVHLRTHTGEKPYECRDCGKSFSHNSSLMIHQRTHTGEKPYECEDCGKGFSHNSGLMIHQRSHTGEKPYECPDCGKTFSQNSNLVKHKRIHTGEKPYECQDCGKSFNVNSILVKHKRTHTGEKPYECPDCGKDFTNKSSLINHVSVHTGEKPFKCPECGRCFSQTSSLKAHKKDSMGEKPYECPDCGKGFSRNCNLTIHQRTHTGEKPYECPDCGKSFSQNSNLIIHQRMHTGEKPYECPDCGKGFSHNSNLVKHQRTHRGEKPYECPDCGKGFSHNSNLMIHQRTHTGEKPYKCLDCGKGFSQNSNLIKHLRTHNREKRYECPDCGECFFHNSNLMIHQEAHTGKKPYECPDCGKSFSNNSNLVKHQRTHTGEKPYECPDCGKSFNQNANLMLHQRTHTGERPYKCLNCGKCFTWYSGLVIHQRTHTGEKPHECPDCGKGFSTRTNLINHVGLHTGEKPFKCSECGKCFSQTSSLVKHKKIHITQKPYRCSECGKSFSYSSNLSSHRKIHTGESPYRCSECGKCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTZ4</accession>
    <name>A0A6I9YTZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger and BTB domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZBTB1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927454.1">
        <property type="nucleotide sequence ID" value="XM_014071979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTZ4"/>
    <dbReference type="GeneID" id="106553474"/>
    <dbReference type="KEGG" id="tsr:106553474"/>
    <dbReference type="CTD" id="22890"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18192">
        <property type="entry name" value="BTB_POZ_ZBTB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF42">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="219"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="574"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="602"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="682"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927454.1"/>
        </source>
    </evidence>
    <sequence length="709" mass="81279" checksum="1D5092ADE943556B" modified="2020-10-07" version="1">MAKTSHSNYVLQQLNNQREWGFLCDCCIAIDDIYFQAHKAVLAACSSYFRMFFMSQQQSTAQLNLSNMKITAECFDLILQFMYLGKIMTAPANFEQFKAAMNSLQLYNMPECLEDIQDTNSSGLQCSSSASSTQNNKMIFGVRMYEDTLAKNKSDANKWSAEPPSSTVSTSQSREVDDEALQVNNFSDQPLESCKRASASKLSGAKDRVPNSRRFGRSFTCDGCGFSFSCERLLDEHIMSCTNRHSYQNPRYLGSENDSSERDSTSKMSVSQLEKYQASANQSAESSSPTASNTTRGKSRGASTEPLNEERSRATERKRIIVKMEPDDNPTDDLKEFNIVKVTNGNDSTDNEELDDDEHVYRYYIEEDVSENRSVRKALLKPHMLIDEQARKYIKSTRVLANKVSANKAKEVTENASCELCGLTITEEDLSSHYLSKHIENICACGKCGQILVKGRQLQDHAQTCGEPQDLTTNGLRNMEEKVDLEESPEEQSENQEMMYVDMLDEFGDSHFQMNTLPKKQLYRDSACPFRCPNCGLRFETENLVVEHMSSCLEQDLFKNAIMEENERDHRRKHFCNLCGKGFYQRCHLREHYTVHTKEKQFVCQTCGKQFLRERQLRLHNDMHKGMARYICSICDQGNFRKHDHVRHMISHLSPGETICQVCFQIFPDNEQLEQHMDVHLYTCGVCGAKFNLRKDMRSHYNAKHLKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU28</accession>
    <name>A0A6I9YU28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Xaa-Pro aminopeptidase 3</fullName>
            <ecNumber evidence="6">3.4.11.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Aminopeptidase P3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">XPNPEP3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013927150.1">
        <property type="nucleotide sequence ID" value="XM_014071675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU28"/>
    <dbReference type="GeneID" id="106553214"/>
    <dbReference type="KEGG" id="tsr:106553214"/>
    <dbReference type="CTD" id="63929"/>
    <dbReference type="OrthoDB" id="4215474at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000013">
        <property type="entry name" value="probable Xaa-Pro aminopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000002">
        <property type="entry name" value="Xaa-Pro aminopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF4">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="15">
        <location>
            <begin position="66"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013927150.1"/>
        </source>
    </evidence>
    <sequence length="504" mass="56995" checksum="5378D353BBF682DC" modified="2020-10-07" version="1">MVWLPAQRLIQPVRVKCNFSACKFCFFRKLSIQPVQQKKIPNRYLGQPSPFTHPHLLKPGEVTPGLSQVEYALRRHKLMAQIQQEVQGTNHTVILLSNPTYYMSNDIPYTFHQDTNFLYLCGFQEPDSILVLQSVPGRSLPYHKALLFVPKRDPSRELWDGPRSGTDGAVALTGVDEAYTMEEFRHLVSKLKDDSSTVWYDVAKPAHPQLHSDYLLHLAEIKAKCNNRVRAVRHLVQNLRLIKSATEIERMKFAAKITSQAFIETMFTSKAPVDEAFLYAKFEFECRARGADILAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCEYSCYVSDVTRTWPINGRFTSPQAELYQAILEVQKSCLRLCSSGVSLENIYNLMLTLIGQKLKELGILKDSTSEGQVFKAVRKYCPHHVGHYLGMDVHDTPDVSRSILLQPSMVITIEPGIYIPEDDTSAPERFRGIGIRIEDDVVVTENVPLILSADCPKEIYDIEQVCSCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU98</accession>
    <name>A0A6I9YU98_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Fibrinogen alpha chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">FGA</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Initiates complement activation and/or interferes in platelet aggregation and/or blood coagulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ficolin lectin family. Veficolin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927611.1">
        <property type="nucleotide sequence ID" value="XM_014072136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU98"/>
    <dbReference type="GeneID" id="106553597"/>
    <dbReference type="KEGG" id="tsr:106553597"/>
    <dbReference type="CTD" id="2243"/>
    <dbReference type="OrthoDB" id="9945370at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072377">
        <property type="term" value="P:blood coagulation, common pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042730">
        <property type="term" value="P:fibrinolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012290">
        <property type="entry name" value="Fibrinogen_a/b/g_coil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF3">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08702">
        <property type="entry name" value="Fib_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01212">
        <property type="entry name" value="Fib_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58010">
        <property type="entry name" value="Fibrinogen coiled-coil and central regions"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1216">Complement system impairing toxin</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="10" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026827954" evidence="15">
        <location>
            <begin position="22"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="16">
        <location>
            <begin position="448"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013927611.1"/>
        </source>
    </evidence>
    <sequence length="691" mass="77818" checksum="7570794BB15FAC5F" modified="2020-10-07" version="1">MFPLRILCALLSFSIALVTSAEPTLEEVRGLEEEIIRGPRIVEHQAQGDCKSDKDWPICGDEHWGIKCPSGCRMQGLIDEANQDFQNRIENIKRALSDNQNSYKSSNNLKQEIFNYLERYLPSEQELNKNYGQISDDLRRRLVTLKQRVADKVERIKTLQNSIHNQVGELKRLEVDIDIKIRACKGSCAHVFNYNVDTESYTNIQKQLVQVNAIKMVSEEEGFPLKTLKITEVKDSPAPIRYKSAALTEQEVSLLDTIKLFKVVLEDPKGSSADPKYAAPEAGVFGKPPISKAIIRPSTDVRTSHTCIKTTTTKIIRGPGGLREETVEEYKTPDGSDCSHLEGFIKEAISSGAGGSGSHDFVPHHPEADSGTLTFSHTKTQEGDPFSEVGEDEFDDFSRFDDFSQGGHTKTIIGTKSRVIETEEIQHDENTERNTGLEEQSFNAEEKFGKGNIGTDCEDIHQKHTSGAQSGIFRITPAGSAKVFSVYCDQETTLGGWLLIQQRLDGSLNFNRTWEDYKKGFGSLDVKGRGEFWLGNENLHILTQKDTVLRVELEDWEGDKVYAEYNINIGSESEGYRLRVSNYKGTAGDALIRGSEEDGTEYTSHTSMKFSTYDRDSDQWEENCAEVYGGGWWYNNCQAANLNGIYYSSGQYDPRNNVPYEIENGVIWLPFRPSDYSLKVVRMKIRPVETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVG3</accession>
    <name>A0A6I9YVG3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Neuroligin-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NLGN1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928603.1">
        <property type="nucleotide sequence ID" value="XM_014073128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVG3"/>
    <dbReference type="GeneID" id="106554464"/>
    <dbReference type="KEGG" id="tsr:106554464"/>
    <dbReference type="CTD" id="22871"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000001">
        <property type="entry name" value="Neuroligin 3 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000460">
        <property type="entry name" value="Nlgn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01090">
        <property type="entry name" value="NEUROLIGIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="15">
        <location>
            <begin position="59"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="676"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="829"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="838"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013928603.1"/>
        </source>
    </evidence>
    <sequence length="848" mass="94831" checksum="E935DC7C79CF01A8" modified="2020-10-07" version="1">MYNSCGTMAFPISVWLNCVWRATQARLLHMGLDATFTFCVLGFLIHTAAVSSQKLDDIDPVVTTNFGKLRGMKKELNNEILGPVIEFLGVPYAAPPIGEHRFQPPEPPSPWPDIKNATQFAPVCPQNIIEGRLPEVMLPVWFTNNLDIVSTFVQDQNEDCLYLNIYVPTEDVKRISKECARKPGKKICRKGDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARMLATKVGCNVSDTVELVECLQKKPYRELVDQDIQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIVDSEDGISASDFDFAVSNFVDNLYGYPEGKDILRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAVATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNFSKNDIMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELVPHLHNLNDISQYTSTTTKVPSTDITFRPTRKNLVPVTSAFPTAKQDDPKQPPSLISGDQRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHDVHRRCSPQRTTANDLTHAQEEEIMSLQMKHSDLGHECESIHPHEVVLRTACPPDYTLAMRRSPDDIPLMTPNTITMIPNTIPGIQPLHTFNTFTGGQNSTLPHPHPHSHSTTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWP7</accession>
    <name>A0A6I9YWP7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trafficking protein particle complex subunit 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106554325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928449.1">
        <property type="nucleotide sequence ID" value="XM_014072974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWP7"/>
    <dbReference type="GeneID" id="106554325"/>
    <dbReference type="KEGG" id="tsr:106554325"/>
    <dbReference type="OrthoDB" id="428342at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928449.1"/>
        </source>
    </evidence>
    <sequence length="664" mass="73620" checksum="52F525E1CF9D9649" modified="2020-10-07" version="1">MEKPESNVEDIGVRSPNAMADICDTQQEASEELLFHEETIDLGGDESASEENGNVADDSSNFIDKLSEHMMESVLISDSPNNSEDDTGDLGCLQGIIKQIEANDTEAIEGEELLERHEVVRNEKDTKLEAAAKSISDSRIDYQSSLNESPIQTPAKNELEGECDKLLKSPSNIVEESKPEEVVSVPVVIKSPDDAEPIPICTIFSQGNQTTGQQQFLHDGFEPQMVKSPSLGAVSETPVKIHPQVVQPSPSLSKFFGEAISNNTLASDFFDSFTTSSFISVSNPNASASVPEQMSSLVTVGELRDSVKHKYALKNGRSEPGKSASSPEISQSPKPFSQIQAVFQSADDPFASVLNMSEVDRRNDAWLPSEATRNVLISVATQPYNNIFIDKENVTMPGLKFDNIQGDAVKDLMLRFLGEQAAAKRQVLNTNSVEQSFQGLKQLISTKNWRAAVDLCGRLLTAHGQGYGKSGLPTSHTTDSLQIWFVRLALLVKLSLYQNAEVEFEPFGNLDQPDLYYEYYPHVYPGRKGSMVPFSMRILHAELQQYLGNPQESLDRLHNMKTICTKILDNLEHGLAEDGSMTNITQESKQGRYPLKLLFQRQDVPITCSEMARTSYKIAQKQRYCHFCSSFSLLKLCTCTLAEIHFPDLPFNFIILIIILILLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX13</accession>
    <name>A0A6I9YX13_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nucleolar protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NOL10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat NOL10/ENP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928501.1">
        <property type="nucleotide sequence ID" value="XM_014073026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX13"/>
    <dbReference type="GeneID" id="106554377"/>
    <dbReference type="KEGG" id="tsr:106554377"/>
    <dbReference type="CTD" id="79954"/>
    <dbReference type="OrthoDB" id="273340at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000601">
        <property type="entry name" value="Nucleolar protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056551">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040382">
        <property type="entry name" value="NOL10/Enp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056550">
        <property type="entry name" value="NOL10_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23098">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23097">
        <property type="entry name" value="NOL10_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nucleolar protein 10-like N-terminal" evidence="10">
        <location>
            <begin position="22"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolar protein 10-like second" evidence="9">
        <location>
            <begin position="150"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="8">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="207"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013928501.1"/>
        </source>
    </evidence>
    <sequence length="469" mass="54904" checksum="ED7F905C437978CB" modified="2020-10-07" version="1">MYLDFPRVGGLPAVSALKFDGSLKMAVGTSTGQVLLYDLRSSNPLIMKDHQYGLPIKSIQFHNALDLIISADSRIIKLWNQDTGKIFTSIEPEHNINDVCLYPNSGMLFTANEAPKMNIYYIPVLGPAPKWCSFLDNLTEELEENPETTVYDDYKFVTRKDLENLGLAHLIGSPLLRAYMHGFFMDIRLYHKVKTMVNPFAYEEYRREKIRQKIEETRAQRVQLKKLPKVNKELALKLIEEGEEELQNARKKKQKKMPNILRDDRFKVMFENPDFQVDEKSEEFRLLNPLVSKMSEKRKKKLKMLEYQEALGEEEEEEPEGKPSDAESSESSDDEKGWVEEVRKQRSLLRQEEKVKRQERFKEDQKTVLEPKFFEIKAGEEFRSFQDTAQKQKLMKKSLGDRLQLEERVGALSVQDTTVGSKQVTFKLKKSEHHKKQQEAEKLHHEERRKLRRSAGHLKSKPGNRRRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX51</accession>
    <name>A0A6I9YX51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CCR4-NOT transcription complex subunit 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928185.1">
        <property type="nucleotide sequence ID" value="XM_014072710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX51"/>
    <dbReference type="GeneID" id="106554108"/>
    <dbReference type="KEGG" id="tsr:106554108"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928185.1"/>
        </source>
    </evidence>
    <sequence length="115" mass="10987" checksum="C2DED0FFE17161BD" modified="2020-10-07" version="1">MGGSSSTSSSLGNGSGSNSNGAVAGSGNASSKANPPAGHASTTPTPYAQAVAPPPPSTNASQPRPPSTQQNASKQNGATSYSSVVADSSSDSTLSSGNQSLPNQTTSHNPPSAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXI2</accession>
    <name>A0A6I9YXI2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">E3 SUMO-protein ligase PIAS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PIAS2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929368.1">
        <property type="nucleotide sequence ID" value="XM_014073893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXI2"/>
    <dbReference type="GeneID" id="106555118"/>
    <dbReference type="KEGG" id="tsr:106555118"/>
    <dbReference type="CTD" id="9063"/>
    <dbReference type="OrthoDB" id="10263264at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000001">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.780:FF:000001">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000003">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF12">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE PIAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="16">
        <location>
            <begin position="11"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="PINIT" evidence="18">
        <location>
            <begin position="134"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="17">
        <location>
            <begin position="331"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="91"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013929368.1"/>
        </source>
    </evidence>
    <sequence length="597" mass="66335" checksum="E8D0405FA46B3BAD" modified="2020-10-07" version="1">MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTIEGLSDLTALKSSVFNLDSSSSPAEPDLAVPGLHPAPSTSVAPQSPSSPLGSVLLQDTKPRFEMQQPSPSVPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCSQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWTSEIGKNYSMSVYLVRQLTSAMLLQRLKLKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAGTCTHLQCFDAALYLQMNEKKPTWICPVCDKKATYENLILDGLFMEILNECSDVDEIKFQEDGSWCPMRPKKEPLKVVSTQCPKIEILHSPGSAMVGKQGSTPGEVSKKKVDVIDLTVESSSDEEEDAPPKRKCIYMSDTQASPTKGVLVYQPAAVRVPGVSTVDSTAIAPSLTDYSVPFHHPPISSISSDLPGLDFLSLIPVDPQSRLDFASKHHLWQSPHLSQPPRQQQQQQQHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZ36</accession>
    <name>A0A6I9YZ36_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Peroxisome proliferator-activated receptor gamma coactivator 1-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PPARGC1B</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929577.1">
        <property type="nucleotide sequence ID" value="XM_014074102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZ36"/>
    <dbReference type="GeneID" id="106555285"/>
    <dbReference type="KEGG" id="tsr:106555285"/>
    <dbReference type="CTD" id="133522"/>
    <dbReference type="OrthoDB" id="10047851at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034605">
        <property type="entry name" value="PGC-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15528">
        <property type="entry name" value="PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA COACTIVATOR 1 PGC-1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15528:SF12">
        <property type="entry name" value="PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA COACTIVATOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="803"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="633"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="648"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013929577.1"/>
        </source>
    </evidence>
    <sequence length="926" mass="103765" checksum="B0D209F56CFBE09B" modified="2020-10-07" version="1">MGLAVFKNMDEGDMPNSGYTSPIPSPNPAAPVTRGPPVAQEVDELSLLKKLLLSPSNVPPSYEVQREGNICRQGPSKSRPQRSCAKVESVHDRKTTFPQAQSRNCTELHKHLTTTTTTRHCPHRKNSGPLDMCHADNITTCSVPQRHYIQNEEDSNLSKDASNCSNSVAPPLSSSEKGTGDKELHATVELIHYMHTYCLPPRKMPPIHPGEGKYQPFHSPSKRAKSDPPLQQSPLCGNDNCPSSPWHFSMHCKQSRTPLSEFSILKELLARELPCDVSKPYRFAKPVYASLARSQLHKSSRTSPHNTGRESTDGYNKPTNKIIPEMKTELRQSLKVEPEAEKGNAEEDGAKQGIHMGQGSYGKSNRKTDTSIYAVRRSKRLNPELSVWQSFLDKPPSEPTVSSAATGNAFASIEPALCSSLQNFDTEAPAAEVEVSGADEMDGGFQSFPMELQTPSPESSRENDTYAANESQCFTPCHQTEAPRCLSLPLAQTESTLGKRSFDQVLTVELCGTAGLTPPTTPPYKPSEEDLYKPDIHQRPAQKTAVITSSEPQPMISHRPISRKHQKKQPVRTELYAHLSRSAIRPAHSEQHGVLKRPFSRSFGDHDYCQVLKPEYSLQRKVLRSWEPLSHIESQHKRQVPDVPYQEESNKSFDKSSVKEGSKQLRDQEIRASLTKHFGLLDSALDDGEEALFFKTPEYDTVFEDSCSEIGSPLEEEEEEEEEEYYMSPLGSNRCLHSSALSRTNLHYCSRNRSDSESSCCRSRSPVNQQVFRRENGKRGQEGNMSSQRLMEKKREKAIGEGRVVYIQNLASSMSSSELKKQFEVFGEIVECCILTKNKRGDKYGVITYRCSEHAALSLKNGASLWKRNEPLFQLAYGGFGHFFWTRFTDLADSNTEESSPTPVKSKYETMDFDSLLQEAQESLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z079</accession>
    <name>A0A6I9Z079_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PTPRK</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013929977.1">
        <property type="nucleotide sequence ID" value="XM_014074502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z079"/>
    <dbReference type="GeneID" id="106555617"/>
    <dbReference type="KEGG" id="tsr:106555617"/>
    <dbReference type="CTD" id="5796"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000003">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000005">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF209">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="191"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="365"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="482"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="658"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929977.1"/>
        </source>
    </evidence>
    <sequence length="748" mass="85240" checksum="FF68A72822AE9836" modified="2020-10-07" version="1">MSSVEKETKTQCVRIATKAAATEEPEVIPDPAKQTDRVVKIAGISAGILVFILILLLVILIVKKSKLAKKRKDAMGNTRQEMTHMVNAMDRSYADQSTIHAEDPLSVTFMDSHNFNPRLPNDPLVPTAVLVPISDENHSATAESSRLLDVPRYLCEGTESPYQTGQLHPAIRVADLLQHINLMKTSDSYGFKEEYESFFEGQSASWDVAKKDQNRTKNRYGNIIAYDHSRVILQPVEDDPSSDYINANYIDGYQRPSHYIATQGPVHETVYDFWRMIWQEQSACIVMVTNLVEVGRVKCYKYWPDDTEVYGDFKVACVEVEPLAEYVVRTFTLERRGYNEIREVKQFHFTGWPDHGVPYNATGLLSFIRRVKQSNPPSAGPIVVHCRCVEKSAGAGRTGCYIVIDIMLDMAEREGVVDIYNCVKALRSRRINMVQTEEQYIFIHDAILEACLCGETAIPVCEFKAAYFDMIRIDSQTNSSHLKDEFQTLNSVTPRLQAEDCSIACLPRNHDKNRFMDMLPPDRCLPFLITIDGESSNYINAALMDSYRQPAAFIVTQHPLPNTVKDFWRLVYDYGCTSLVMLNEVDLAQGCPQYWPEEGMLRYGPIQVECMSCSMECDVINRIFRICNLTRPQEGYLMVQQFQYLSWAPHRDVPGSKRSFLNLILQVEKWQEECEEGEGRTIIHCLNGGGRSGMFCAIGIVVEMVKRQNVIDVFHAVKTLRNSKPNMVETPEQYRFCYDVALEYLESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z085</accession>
    <name>A0A6I9Z085_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PPP2R3C</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929270.1">
        <property type="nucleotide sequence ID" value="XM_014073795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z085"/>
    <dbReference type="GeneID" id="106555030"/>
    <dbReference type="KEGG" id="tsr:106555030"/>
    <dbReference type="CTD" id="55012"/>
    <dbReference type="OrthoDB" id="10265007at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001782">
        <property type="term" value="P:B cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030865">
        <property type="term" value="P:cortical cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045579">
        <property type="term" value="P:positive regulation of B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043029">
        <property type="term" value="P:T cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21505">
        <property type="entry name" value="PPP2R3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039865">
        <property type="entry name" value="PPP2R3C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PP2A regulatory subunit B'' EF-hand" evidence="9">
        <location>
            <begin position="180"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929270.1"/>
        </source>
    </evidence>
    <sequence length="410" mass="48330" checksum="2DD1D7628A415E59" modified="2020-10-07" version="1">MDWKQALKGRVVVSTDTKRSDQELKDDEMESFTKYYLKWKGGRRSASTSNVNIPRFYYRLPAEDEILLQKLREESRAVFLQRKSRELLDNEELQNLWFLLDEHQTPPMMGEEAMISYENFLKVGEKAGSKCKQFFTAKIFTKLLHSDPNGRISIMQFFNYVMRKVWLYQTRIGLSLYDVAGQGYLRESDLENYILELIPTLPQLDGLEKSFYSFYVCTAVRKFFFFLDPLRTGKIKIQDILACSFLDDLLELRDEELSKESQETNWFSAPSALRVYGQYLNLDKDHNGMLSKEELSRYGTGTLTNIFLDRVFQECLTYDGEMDYKTYLDFVLALENRKEPAALQYIFKLLDIENKGYLNVFSLNYFFRAIQEQMKIHGQDPVSFQDVKVRILIKMYLYAILSELTFEVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0F2</accession>
    <name>A0A6I9Z0F2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homeobox protein DLX-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DLX3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930468.1">
        <property type="nucleotide sequence ID" value="XM_014074993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0F2"/>
    <dbReference type="GeneID" id="106556033"/>
    <dbReference type="KEGG" id="tsr:106556033"/>
    <dbReference type="CTD" id="1747"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000048">
        <property type="entry name" value="Distal-less homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022135">
        <property type="entry name" value="Distal-less_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF28">
        <property type="entry name" value="HOMEOBOX PROTEIN DLX-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12413">
        <property type="entry name" value="DLL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="126"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="128"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="194"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="197"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930468.1"/>
        </source>
    </evidence>
    <sequence length="247" mass="27986" checksum="B1D68E249042846E" modified="2020-10-07" version="1">MSGPFDKKISSILTDLSSCHAGTKDSPTLPESSITDLGYYSTSQNQHEYYPSQAYAQPMNPYAYHPQFNFNGMGATGTFSPKSDYPYSTSYRQYGSFRDQQLPSQETVSVKEEPEPEVRMVNGKPKKIRKPRTIYSSYQLAALQRRFQKAQYLALPERAELAAQLGLTQTQVKIWFQNRRSKFKKLYKNGEVPLEHSPNNSDSMACNSPPSPTLWDSNTHNNPATRSQIPQPLSYISPSSYLEDQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0V6</accession>
    <name>A0A6I9Z0V6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Syntaxin-1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106555393</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929711.1">
        <property type="nucleotide sequence ID" value="XM_014074236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0V6"/>
    <dbReference type="GeneID" id="106555393"/>
    <dbReference type="KEGG" id="tsr:106555393"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15848">
        <property type="entry name" value="SNARE_syntaxin1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000042">
        <property type="entry name" value="Syntaxin 11b, tandem duplicate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF84">
        <property type="entry name" value="SYNTAXIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="16">
        <location>
            <begin position="192"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="68"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013929711.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33924" checksum="6D858989160A27F9" modified="2020-10-07" version="1">MKDRTQELRTAKDSDDDDEVAVGVDRDRFMDEFFEQVEEIRGFIEKISENVEEVKRKHSAILAAPNPDEKTKEELEDLMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNTTQTDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFSSGIIMDSNITKQALNEIETRHSEIIKLENSIRELHDMFMDMAMLVESQVRAAAEGWTSLLMAHQVATFSPVLISLRSALSSARDTAMVPLHYSSYIKKRVGINDFVSSMAWITQINFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1K7</accession>
    <name>A0A6I9Z1K7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Actin-85C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106555790</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930174.1">
        <property type="nucleotide sequence ID" value="XM_014074699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1K7"/>
    <dbReference type="GeneID" id="106555790"/>
    <dbReference type="KEGG" id="tsr:106555790"/>
    <dbReference type="OrthoDB" id="6953074at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13397">
        <property type="entry name" value="ASKHA_NBD_actin_Arp-T1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000002">
        <property type="entry name" value="Muscle actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930174.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="40766" checksum="838D130DC4575AE2" modified="2020-10-07" version="1">MSQSRSAVIIDNGSGICKAGFSGEEAPRAMVATVVGYPKTRAIMFGPVQRTCYVGKEAQAKRGILSFKYPVEHGIVTSWDDMEKIWRYIYRHGLKARAKERPVLLTEAPMNPKGNREKMTEIMFEHFEVPAMFVGLQALMALYASARTTGLVLDSGAGVTLTVPIYKGHCLLHAISRMNFAGRDVTRYLATLLLESGHNFLSSAEKEIVKNIKENLCYVALDSGHPKDKASIRKYTLPDGTPVEIGDQLFRAPEALFNPTDAGVAAPGIHQMIAESISKCEGSIRHDLWRSLIVAGGSTLFPGLSERLLKELRSLGPRGLPTNLEAPANRIHSVWIGASVLTSLPSFQNMWVTQEEYREVGSTVLQKKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3B8</accession>
    <name>A0A6I9Z3B8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Isthmin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ISM2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isthmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930719.1">
        <property type="nucleotide sequence ID" value="XM_014075244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3B8"/>
    <dbReference type="GeneID" id="106556256"/>
    <dbReference type="KEGG" id="tsr:106556256"/>
    <dbReference type="CTD" id="145501"/>
    <dbReference type="OrthoDB" id="9930623at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000033">
        <property type="entry name" value="Isthmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051867">
        <property type="entry name" value="Angio_Inhib/Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239">
        <property type="entry name" value="ISTHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239:SF28">
        <property type="entry name" value="ISTHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="AMOP" evidence="7">
        <location>
            <begin position="259"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013930719.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="49969" checksum="A6179EB0C7DC9F28" modified="2020-10-07" version="1">MPQSHRMQNLRLNGQVIPDQHKHHWPLQQASRNQLASLQPAEILPEEKTPFVLDLQSLTGLANVDLNAQNPNIQVTIEVVNDPQAEMEMDLLKETSNDWSLSSSDWLSHKDLFWPLFWEYTDPIEDGDDNLNTKSRDEEEEEEEEEEEEEEDYTSEYDEEEIVLSGVGGNWHQRWPNRKNWILKEKYNYDYEDEEDWSPWSSCSVTCSNGHQKRTRSCGYACTATESRTCELQYCPGEVGELASTTEETPSEAENATEILNADVDSCEKWLTCKSDFLNKYLSKVLSDLPNCPCAYPLEAVYNAVNLQDEQKDKNFRWQDASGPKERLDIYKPTAHFCLRSMLSLDSTTLGAQHCCYDENTKLITRGKGAGAPNLISTEFSPELHYKVDMLPWILCKGDWSRYHAVRPPNNGQQCAVNPPEDEYLSQLQEAREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4L4</accession>
    <name>A0A6I9Z4L4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Long-chain-fatty-acid--CoA ligase ACSBG2</fullName>
            <ecNumber evidence="10">6.2.1.15</ecNumber>
            <ecNumber evidence="11">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Acyl-CoA synthetase bubblegum family member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Arachidonate--CoA ligase ACSBG2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106556325</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z)-octadecenoate + ATP + CoA = (9Z)-octadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33607"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33608"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33651"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:33652"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.15" evidence="10"/>
    <dbReference type="EC" id="6.2.1.3" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013930795.1">
        <property type="nucleotide sequence ID" value="XM_014075320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4L4"/>
    <dbReference type="GeneID" id="106556325"/>
    <dbReference type="KEGG" id="tsr:106556325"/>
    <dbReference type="OrthoDB" id="3633556at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF101">
        <property type="entry name" value="ACYL-COA SYNTHETASE BUBBLEGUM FAMILY MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="20">
        <location>
            <begin position="45"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="22">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013930795.1"/>
        </source>
    </evidence>
    <sequence length="526" mass="58165" checksum="1BBDF782A990E4CF" modified="2020-10-07" version="1">MSKGPPPGAGGLWTTKRDGEVKLRMETSGIGSEPPITVHDLFLATVEKYGHFPALASKKQGHWSKLTFRQYYEECRKTAKSFLKLGLGRFNSVCILGFNSLEWFLADIGAIFAGGFAVGIYTTNSAEACHYVAENCGANIIVLENDKQLQKILEVEKKLPLLKAIIYYNDDIKEKRPNLYSWEEFMALGSSVPDEQLDKVVASLKPNQCCTIIYTSGTTGNPKGVMLSHDNITWTAKAAGEYVHLKTALEGQESVVSYLPLSHIAAQMVDIWLPVTFGVETYFAQPDALKGSLVDTLREVRPTAFMGVPRVWEKMQERMKSVGAKSSTLKKKIAAWAKAVGLETNLKRMNGSVDLPMNYRLARALIYKKVRKALGLDRCDKCYTGAAPITKDTLEYFLSLDIVVYELYGMSESSGPHTVSFPSSYRMASCGKEVTGCQTMLFKPDQEGVGEVCFAGRHIFMGYLNMEDKTKEAIDGEGWLHSGDLGRHDEDGFLYITGRIKELIITAGGENIPPVPIEDAIKEAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4Z7</accession>
    <name>A0A6I9Z4Z7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RAB33B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013930935.1">
        <property type="nucleotide sequence ID" value="XM_014075460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4Z7"/>
    <dbReference type="GeneID" id="106556467"/>
    <dbReference type="KEGG" id="tsr:106556467"/>
    <dbReference type="CTD" id="83452"/>
    <dbReference type="OrthoDB" id="10006973at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04115">
        <property type="entry name" value="Rab33B_Rab33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000516">
        <property type="entry name" value="RAB33B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041822">
        <property type="entry name" value="Rab33A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013930935.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25596" checksum="9A03244629BB0BFB" modified="2020-10-07" version="1">MAADVDSSLELSLSNSYAGPGSLRSARSRIFKIIVIGDSNVGKTCLTYRFCAGRFPERTEATIGVDFRERAVDIGDERIKIQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDMTNIASFHSMPLWIEECKQHLLTSEIPRILVGNKCDLRNVIQVPTDVAQKFADTHGMPLFETSAKNPNDNDHVEAIFMTLAHKLKSHKPLMLSQLPENTIHLKPASKPTMTCWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z583</accession>
    <name>A0A6I9Z583_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adenosylhomocysteinase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106556675</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931126.1">
        <property type="nucleotide sequence ID" value="XM_014075651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z583"/>
    <dbReference type="GeneID" id="106556675"/>
    <dbReference type="KEGG" id="tsr:106556675"/>
    <dbReference type="OrthoDB" id="10007170at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000007">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF2">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="5">
        <location>
            <begin position="163"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013931126.1"/>
        </source>
    </evidence>
    <sequence length="224" mass="24613" checksum="562BF691E0AAAAE9" modified="2020-10-07" version="1">MPALMALRKRAQGEKPLAGAKIVGCTHITAQIAVLMETLGALGAQCRWAACNIYSTLNEVAAALAENGFPVFAWKGESEDDFWWCIDRCVNVEGWQPNMILDDGGDLTHWIYKKYPNMFKKIKGIVEESVTGVHRLYQLSKAGKLCVPAMNVNDSVTKQKFDNLYCCRESIREGLKRTTDMMFGGKQVVVCGYGEVGKGCCAALKAMGSIVYVTEIDPICALQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z592</accession>
    <name>A0A6I9Z592_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Vesicle-associated membrane protein-associated protein B/C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">VAPB</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. Interacts with STARD3 in a FFAT motif phosphorylation dependent manner. Via interaction with WDR44 participates in neosynthesized protein export. Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931822.1">
        <property type="nucleotide sequence ID" value="XM_014076347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z592"/>
    <dbReference type="GeneID" id="106557153"/>
    <dbReference type="KEGG" id="tsr:106557153"/>
    <dbReference type="CTD" id="9217"/>
    <dbReference type="OrthoDB" id="264603at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033149">
        <property type="term" value="F:FFAT motif binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000334">
        <property type="entry name" value="vesicle-associated membrane protein-associated protein A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF12">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="16">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013931822.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="27351" checksum="F15584519DA35712" modified="2020-10-07" version="1">MAKTEQVLILEPQHELKFKGPFTDVVTTNLKLGNSTDKNVCFKVKTTAPRRYCVRPNSGIIDAGTSINVSVMLQPFDYDPNEKSKHKFMVQSMFAPPDTSDMEAVWKEAKPEELMDSKLKCVFELPAENDKPHDIEINKIISPIASKTESSIMSKSLSSSSDDSELKKVMEECKRLQLEVQRLREENKQFKEEDGLRMRKAPQSNHPSYASATTVKEEGLSTRLLALVVLFFIVGVIIGKIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7K0</accession>
    <name>A0A6I9Z7K0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calcyphosin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CAPSL</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931790.1">
        <property type="nucleotide sequence ID" value="XM_014076315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7K0"/>
    <dbReference type="GeneID" id="106557158"/>
    <dbReference type="KEGG" id="tsr:106557158"/>
    <dbReference type="CTD" id="133690"/>
    <dbReference type="OrthoDB" id="444540at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013931790.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="18397" checksum="36C2B7B83E84FB57" modified="2020-10-07" version="1">MDDDKSRTLDFKEFTKGLHDYAVLIDKAEAEKIFQTFDKDGNGTIDFDEFLLTLRPPMSNSRKEVIMQAFRKLDKTGDGVITIEDLQGVYNVKHHPKYQNGEWSEEQVFRTFLDNFDSPYDKDGQVTTEEFMNYYAGVSASIDTDVYFIVMMKNAWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7S3</accession>
    <name>A0A6I9Z7S3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Platelet-activating factor acetylhydrolase IB subunit alpha1</fullName>
            <ecNumber evidence="2">3.1.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">PAF acetylhydrolase 29 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">PAF-AH subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PAFAH1B3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Alpha1 catalytic subunit of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)) heterotetrameric enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and modulates the action of PAF. The activity and substrate specificity of PAF-AH (I) are affected by its subunit composition. Both alpha1/alpha1 homodimer (PAFAH1B3/PAFAH1B3 homodimer) and alpha1/alpha2 heterodimer(PAFAH1B3/PAFAH1B2 heterodimer) hydrolyze 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid (AAGPA) more efficiently than PAF, but they have little hydrolytic activity towards 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylethanolamine (AAGPE). Plays an important role during the development of brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphate + H2O = 1-O-hexadecyl-sn-glycero-3-phosphate + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:77580"/>
            <dbReference type="ChEBI" id="CHEBI:78385"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:41705"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40479"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:44811"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:40480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36707"/>
            <dbReference type="EC" id="3.1.1.47"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17778"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Forms a catalytic dimer which is either homodimer (alpha1/alpha1 homodimer) or heterodimer with PAFAH1B2 (alpha1/alpha2 heterodimer). Component of the cytosolic (PAF-AH (I)) heterotetrameric enzyme, which is composed of PAFAH1B1 (beta), PAFAH1B2 (alpha2) and PAFAH1B3 (alpha1) subunits. The catalytic activity of the enzyme resides in the alpha1 (PAFAH1B3) and alpha2 (PAFAH1B2) subunits, whereas the beta subunit (PAFAH1B1) has regulatory activity. Trimer formation is not essential for the catalytic activity. Interacts with VLDLR; this interaction may modulate the Reelin pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.47" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013931860.1">
        <property type="nucleotide sequence ID" value="XM_014076385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7S3"/>
    <dbReference type="GeneID" id="106557215"/>
    <dbReference type="KEGG" id="tsr:106557215"/>
    <dbReference type="CTD" id="5050"/>
    <dbReference type="OrthoDB" id="505607at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047179">
        <property type="term" value="F:platelet-activating factor acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01820">
        <property type="entry name" value="PAF_acetylesterase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000004">
        <property type="entry name" value="Platelet-activating factor acetylhydrolase IB subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11852">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11852:SF2">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE IB SUBUNIT ALPHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="18">
        <location>
            <begin position="20"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013931860.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22760" checksum="4786617201A285B8" modified="2020-10-07" version="1">MSLHHRFIADSKDKEPEVVFIGDSLVQLLHQFEIWRELFSPLHALNFGIGGDATQHVLWRLQNGELQHIRPKIVVLWVGTNNHGHTAEQVAAGIESIVRVINQQQPQARVVVLGLLPRGKNPNPLREKNRRVNELLESKMPLLPNASFLNVDPGFVHSDGTISHHDMYDYLHLTRNGYARLCPGLHRLLLCLLGECHAQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WVV2</accession>
    <name>A0A6I9WVV2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine incorporator 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SERINC5</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907049.1">
        <property type="nucleotide sequence ID" value="XM_014051574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WVV2"/>
    <dbReference type="GeneID" id="106537442"/>
    <dbReference type="KEGG" id="tsr:106537442"/>
    <dbReference type="CTD" id="256987"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF16">
        <property type="entry name" value="SERINE INCORPORATOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013907049.1"/>
        </source>
    </evidence>
    <sequence length="462" mass="51624" checksum="25447B3B2942C494" modified="2020-10-07" version="1">MSARCCAGQLACCCGTAGCSLCCSCFPKIKQSTSTRFMYALYFILVALISCIMMSETVAKQMKEHIYFYETVCQHIQAGDSCEKLVGYSAVYRVCFGTACFFFIFFLLTININNSKTCRAYIHNGFWFIKLLILAGMCSGAFFIPDQDTFLKAWRYVGATGGFLFIIIQLILLVEFAHKWNKNWTSGTKHNQLWYGSLALVTLVLYSVAVGALAVMAVFYTSADGCTFNKILLGINGGLCLLISMVAISPCVQNRQPHSGLLQSGIISCYVMYLTFSSLSSKPPEIILDENQNNKTICAPDFGQGLQTDENLVTGLGTTILFCCILYSCLTSTTRASSEALRGIYVTPETEIARCCFCCAPDGVAEVEEHIGRKGGQRVIYDEKKGTVYSYAYFHFVFFLASLYVMMTVTHWFHYENAAIEKFFVGTWSIFWIKMASCWVCVLLYLWTLLAPLCCPTRGFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X117</accession>
    <name>A0A6I9X117_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA-binding protein 38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RNA-binding motif protein 38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106538142</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RBM38 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908036.1">
        <property type="nucleotide sequence ID" value="XM_014052561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X117"/>
    <dbReference type="GeneID" id="106538142"/>
    <dbReference type="KEGG" id="tsr:106538142"/>
    <dbReference type="OrthoDB" id="4207594at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF28">
        <property type="entry name" value="RNA-BINDING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="34"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013908036.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="24532" checksum="59BD7AEE9C5228B4" modified="2020-10-07" version="1">MHRRRRQQQQHRRPLQKRTARPPAKIASPSRRGRKYFEVFGDIDEAVVITDRQTGKSRGYGFVTMTDRAAAERACKDPNPIIDGRKANVNLAYLGAKPRSIQTGFTLGVQQLHPALIQRPYGLAHHFIYPQAIVQPSVVIPTPVQSITSPYIDYTTASQAYSQFTAAAYDQYPYAPSPAAGFVGYGYTGGLPQPTTAAGLPIAAPTTFVQYPATQLQPDRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X264</accession>
    <name>A0A6I9X264_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metalloprotease TIKI</fullName>
            <ecNumber evidence="13">3.4.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">TRAB domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106538063</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the N-terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="13">Divalent metal cations. Mn(2+) or Co(2+).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TIKI family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013907922.1">
        <property type="nucleotide sequence ID" value="XM_014052447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X264"/>
    <dbReference type="GeneID" id="106538063"/>
    <dbReference type="KEGG" id="tsr:106538063"/>
    <dbReference type="OrthoDB" id="10040378at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14789">
        <property type="entry name" value="Tiki"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040230">
        <property type="entry name" value="TIKI1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31120">
        <property type="entry name" value="METALLOPROTEASE TIKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31120:SF8">
        <property type="entry name" value="METALLOPROTEASE TIKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013907922.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="24091" checksum="5719A4EE7A62E95A" modified="2020-10-07" version="1">MRRQQMDPRRCQLPRSQRDLNSFLWTIRRDPPAYLFGTIHVPYTRVWDFVPENSKAAFQASSSVYFELDLTNPSTISGLANCQLLPRGENLQDVLPRDLYHRLKRHLDYVRLMLPHWMTPDQRGKGLYADYLFNAIAGNWERKRPVWVMLMVNSLTETDVRSRGVPVLDLHLAQEAERMKKGTGAVEKVEEQCHPLNGLNFSQVRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2X9</accession>
    <name>A0A6I9X2X9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homeobox protein DLX-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DLX2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909639.1">
        <property type="nucleotide sequence ID" value="XM_014054164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2X9"/>
    <dbReference type="GeneID" id="106539378"/>
    <dbReference type="KEGG" id="tsr:106539378"/>
    <dbReference type="CTD" id="1746"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000048">
        <property type="entry name" value="Distal-less homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022135">
        <property type="entry name" value="Distal-less_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF23">
        <property type="entry name" value="HOMEOBOX PROTEIN DLX-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12413">
        <property type="entry name" value="DLL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="141"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="206"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013909639.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="34099" checksum="69D02C13A77AA280" modified="2020-10-07" version="1">MTGVFDSLVSDMHSNQITSSSYQQQQHHSLHKPQESPTLPVSTATDSSYYTNQQQHSGGAGGGTGSNGSSGSPYTQMGSYQYHPNGIGAVSYSTKAGSYDLGYPTSYSSYATYGTRTPPINNEPEKEEVEPEIRIVNGKPKKVRKPRTIYSSFQLAALQRRFQKTQYLALPERAELAASLGLTQTQVKIWFQNRRSKFKKMWKSGEIPGDQQRVSSSSPPCPSPPASATLTWDFAPHPQRMSSGGGGSSSAGSSPGTPSGATAFLGNYSWYHQASSAAASHLPGASPILQQGQSHQHHPHLHHAHHHGATVSAGTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X414</accession>
    <name>A0A6I9X414_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GPI transamidase component PIG-T</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PIGT</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910054.1">
        <property type="nucleotide sequence ID" value="XM_014054579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X414"/>
    <dbReference type="GeneID" id="106539715"/>
    <dbReference type="KEGG" id="tsr:106539715"/>
    <dbReference type="CTD" id="51604"/>
    <dbReference type="OrthoDB" id="331263at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007245">
        <property type="entry name" value="PIG-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959:SF11">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04113">
        <property type="entry name" value="Gpi16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026955615" evidence="1">
        <location>
            <begin position="23"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910054.1"/>
        </source>
    </evidence>
    <sequence length="577" mass="65132" checksum="529E2FD4B3D07E03" modified="2020-10-07" version="1">MEVAVSLLGLLLWASGSAESAAEPPRDSLREELVLSPLPSGDVAATFQFRTRWDSDLQRGGVSHYRLFPKALGQLISKYSVRELHLSLTQGFWRTRSWGQPYLQAPAGAELWVWFQDTVSDVDKAWKDLSNILSGIFCASLNFIDSTNTIIPTASFKPLGLVNITDHRFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLAVLLKAERLFHSSYHSQAVHIRPICRDKSCLSLSWELRQILTVVFDSFASAQGKKDWSLLKIFSRTLTEACPLASQSKVYVDISSKTKAQEFLEVSPTPLSVYEGSVQGDRRTYAVYDLLNPTLFNTSRNLNVLLKWKRPQDNLGLAVPVLHAQRYVSGYGLQTGEISTLVYNTHPYRAFPVLLLETVPWYLRLYIHTLTIITKGKENKPSYIHYQPAKDRKRPHLLEMLIQLPANSVTKINIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSTIAIKEEDEDTKPLFASLFPSFDGASYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHVEEPKKGGLAKRLANIIRKIRGVPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4T6</accession>
    <name>A0A6I9X4T6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Src-like-adapter 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SLA2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908877.1">
        <property type="nucleotide sequence ID" value="XM_014053402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4T6"/>
    <dbReference type="GeneID" id="106538786"/>
    <dbReference type="KEGG" id="tsr:106538786"/>
    <dbReference type="CTD" id="84174"/>
    <dbReference type="OrthoDB" id="9924021at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="2">
        <location>
            <begin position="95"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908877.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="33883" checksum="7DFB13E53606DC0B" modified="2020-10-07" version="1">MGSLVCKRKQIANQTSLEVNTRHSQSDSVHLPVANSPLIIAVSDFPSRYREPVLRMGEQLNLLSEEGEWCQVKSVATGKECCVPSNNIAKISHRWLYEGIDRAKAEELLLLPANQEGSFIIRKRQAKKGCYSLSIRCTNHESWNCVKHYRINCLENNWLYISPSLTFPSLHELVDYYSESSDGLCCCLKKPCFIQGASSSLFHNLPKPVEVKRSALNWQQIKRLFYFLHSTYNQPLSMEPVPMSKRLGVTVLHCFYTILKTTHENSCFFSTPESSAVTNINHWSKKLCCYKGKNSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8H4</accession>
    <name>A0A6I9X8H4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Kelch repeat and BTB domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">KBTBD4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910626.1">
        <property type="nucleotide sequence ID" value="XM_014055151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8H4"/>
    <dbReference type="GeneID" id="106540126"/>
    <dbReference type="KEGG" id="tsr:106540126"/>
    <dbReference type="CTD" id="55709"/>
    <dbReference type="OrthoDB" id="2311693at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18272">
        <property type="entry name" value="BTB_POZ_KBTBD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042884">
        <property type="entry name" value="KBTBD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042949">
        <property type="entry name" value="KBTBD4_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47195">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47195:SF1">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013910626.1"/>
        </source>
    </evidence>
    <sequence length="543" mass="60844" checksum="8CD29137977B95DB" modified="2020-10-07" version="1">MKGGIANYWRSNHSNNMDSLQETGGSSTGDTGSSSNEENYFMNYTFTDRSHSSRVAQGIMKLCLEEELFADVTISVEGKEFQLHRLVLSAQSCFFRSMFTSNLKEAHNRVIELQDVSESVFQLVVDYIYHGTVKLRADYLQETYEAADMYQLTALFEECSRFLARTVQVKNCLQIMWLADQHSDVELYTAAKHCAKSYFAQLQETEEFLHLPLRFLTDIVSDGVPCSQKPTAAIETWIGFNKEERAGFLELLQSSLKEIGENVHIYLIGKESSRTHSLAVSLHCADDDSISVSGQNSLCHQITAACKHGSDLYVVGGSIPRRMWKCNNTTIDWEWCAPLPRDRLQHTLVSVLNKDAIYSLGGKTLQDILSNAIIYYKVKDNVWTETSQLEVAVSGAAGVNLNGVIYLLGGEENDADFFTKPSRLIQCYDTKTEKCYVKPYVLPFAGCMHAAVYKDLVFIVAQGDSLLCYNPLLDSFTRLCLADAWSSVPSPWKIASCNGNIYIFRDCYKKGDASTFKLNPATSVVSVTRGLKVLLTNLQFVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X979</accession>
    <name>A0A6I9X979_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Interferon-stimulated 20 kDa exonuclease-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ISG20L2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910876.1">
        <property type="nucleotide sequence ID" value="XM_014055401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X979"/>
    <dbReference type="GeneID" id="106540324"/>
    <dbReference type="KEGG" id="tsr:106540324"/>
    <dbReference type="CTD" id="81875"/>
    <dbReference type="OrthoDB" id="16516at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06149">
        <property type="entry name" value="ISG20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037433">
        <property type="entry name" value="ISG20_DEDDh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF78">
        <property type="entry name" value="INTERFERON-STIMULATED 20 KDA EXONUCLEASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="7">
        <location>
            <begin position="170"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013910876.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="38488" checksum="232B2018C9315AD7" modified="2020-10-07" version="1">MSDILLNLETVLPPPPAQKGPAGGNKKHQRFVKRRQFLERKGFLKQKQLPPQPPPPRRDASLSCPAGPHRPKKKQRVEAKEGGKPSLEERPPCRLKVPPKETSGSGAVDPTSWTSGSSAAAAASSSRKTGSCAGKPNGTLCSRGDVPRVNLLSEFKSGLPDAPGLAKPSKMVAIDCEMVGTGPGGRNSDLARCSVVGYYGDVMYDKYIRPLSPITNYRTRWSGIRQHHMRDAVPFKLAQKEILKLLSGKIVIGHAIHNDFKALKYFHPKSLTRDTSKIPLLNRKAGFPENESASLKRLTKQLLLRDIQVGHNGHSSVEDARATMELYKVIEVEWERHLATSPLQDGPPDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAI8</accession>
    <name>A0A6I9XAI8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">T-cell surface glycoprotein CD3 epsilon chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CD3E</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911326.1">
        <property type="nucleotide sequence ID" value="XM_014055851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAI8"/>
    <dbReference type="GeneID" id="106540658"/>
    <dbReference type="KEGG" id="tsr:106540658"/>
    <dbReference type="CTD" id="916"/>
    <dbReference type="OrthoDB" id="9947847at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042105">
        <property type="term" value="C:alpha-beta T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045059">
        <property type="term" value="P:positive thymic T cell selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015484">
        <property type="entry name" value="CD3_esu/gsu/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10570:SF9">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD3 EPSILON CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10570">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD3 GAMMA CHAIN / DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16681">
        <property type="entry name" value="Ig_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026875244" evidence="6">
        <location>
            <begin position="23"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013911326.1"/>
        </source>
    </evidence>
    <sequence length="182" mass="19995" checksum="2BC1E28638FEF4FD" modified="2020-10-07" version="1">MPLALHLGALVLLLFHTASLEAGEIKEITVEIASGKVFLTCPEEKSEWWDKKSDLGTSRILDISAPDDLLEIERGKEFSCGEKKSKSWRIYLKVKGCKDCVELSLGLGAGIIVADLLLTLGVLLLVYFCSQKQPGLFRPGAPRRQRLQGQQADHPPPVPNPDYEPIRKGQGEVYAGLAPQAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCL9</accession>
    <name>A0A6I9XCL9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Bromodomain and WD repeat-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BRWD1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013908898.1">
        <property type="nucleotide sequence ID" value="XM_014053423.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106538804"/>
    <dbReference type="KEGG" id="tsr:106538804"/>
    <dbReference type="CTD" id="54014"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05529">
        <property type="entry name" value="Bromo_WDR9_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05496">
        <property type="entry name" value="Bromo_WDR9_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000017">
        <property type="entry name" value="Bromodomain and WD repeat domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000023">
        <property type="entry name" value="Bromodomain and WD repeat domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000071">
        <property type="entry name" value="Bromodomain and WD repeat domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000066">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052060">
        <property type="entry name" value="Bromo_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057451">
        <property type="entry name" value="BRWD/PHIP_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057452">
        <property type="entry name" value="BRWD/PHIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266:SF26">
        <property type="entry name" value="BROMODOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266">
        <property type="entry name" value="WD REPEAT DOMAIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25437">
        <property type="entry name" value="BRWD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25313">
        <property type="entry name" value="BRWD_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="301"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="399"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="500"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="1203"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="1355"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="870"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="934"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1481"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1552"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1613"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1678"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1858"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1996"/>
            <end position="2018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2030"/>
            <end position="2069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2122"/>
            <end position="2167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2183"/>
            <end position="2203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2298"/>
            <end position="2323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2369"/>
            <end position="2542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="878"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="890"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="941"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1489"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1555"/>
            <end position="1588"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1613"/>
            <end position="1622"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1715"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1749"/>
            <end position="1759"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1870"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1901"/>
            <end position="1910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1996"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="2049"/>
            <end position="2062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2122"/>
            <end position="2137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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        <location>
            <begin position="2313"/>
            <end position="2323"/>
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    </feature>
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        <location>
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    </feature>
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            <begin position="2510"/>
            <end position="2531"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908898.1"/>
        </source>
    </evidence>
    <sequence length="2542" mass="289449" checksum="F8C147D41D3BBF15" modified="2020-10-07" version="1">MSSQAWAAAPDRALSLNLDLATAAATEMAELSKASNSALAAGGLSMSLIESELYFLIARFLGAGPCRKAAEVLVRELEQYQLLPKRLDWEGKEHYRRYEDLVLSNKHVAPDHLLQICKRIGPLLDKEIPPSIPRVNSLLGAGKQSLLRTAKDCKYTNWKGSGIAALHRGRPPEIPINCGRKPSLVELNYAKQLTGCTRYSCSFPENMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWCTHNGRLLATLRGHSAEISDMAVNFENTMIAAGSCDKIIRVWCLRTCAPIAVLQGHTGSITSLQFSPLVKGSIRYMASTGVDGTVCFWQWDTISMKFNNRPLKFTEKPRPGVQMLCSSFSVGGMFLVTGSTDHVIRIYYFGSETPEKIAELESHADKVDSIQFSNRDDRFISGSRDGTARIWRFQQAEWKSILLDMADKLPSSDLCSEEDKFMKPKVTMIAWSRNDNVVVTAVNNHLLKVWNSISGQLLHDLKGHADEVFVLEPHPFDSRIMLSAGHDGNIFIWDIEKGIQMKHYFNMIEGQGHGAVFDCKFSSDGQHFTCTDSHGHLLIFGFGCNKSYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENCADEHLIPQLGYIATSDGEVIEQVIGQQTAEQDEQNLEPSILDGMIRQLQQLQDQRISIDQDIVSANPHYGQETPRRDFRRLSSDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWRRRVVVPEITFSIFRNQEEYRISKGEEEKWLYTIEQKRKLFQSSEKSDSEDSLMESKRRLFRRKYASYQSRNNIEQSDSSSEDIGDSFGFIDQDAEESDGLSLTSREEEWKSDKKSSSASSSDSSSQYSDWIADAGINLHPPVRTSQRKIIRYCISSEDEVSIEKNSPPKRRKRRKKYKPKNHESADQTLSTDFQPPDWITNIRLRRSPFVPQMGDEVIYFRQGHEAYIEAVRKNNIYELNPQKEPWRKMELRDQELVKIVGIRYEVGPPTLCCLKLNLMDHVTGQLQDKSFSLKYHDMPDVIDFLVLRQFYDEALQRNWQPYDRFRSIIDDAWWFGIVLSQEPYQPQYPDSPFQCYSVKWDNGETERLSPWDMEPIPENVDQPKELGASVSVTAEEMAKLLYKPQKGEWQGNSQSEECERIISGIQQLLRLDISAAFAGPVDLYTYPKYSTVVAYPTDLWTIQLRLTNRFYRRLSALIWEVRCIESNARTFNEPNSKIAKTAQKITDQLLEFIRNPNCTNFFELCTATNEDSIAATESDDDYKLRTSSRKRQLLKGKNITKSRYDENRWRKQCMELVNLIFQCEDSEPFRQPVDLERYPDYREIIDTPMDLATIKETLEAENYDTPMELCKDIRLIFSNAKAYTPNKKSKIYSMTLRLSALFEEKISRIISDFRTGQKHNERIWRSERSSRKLQSLNKSVQRSAKILRNAKQKQCKSQTEIQSEAENSSNQNTSGKKSCVTSHKVYTTSCTRSIAGMSSDSTSASYFGSNTRTRNTAVAHSSTLSTESELEHSSTTSSLSSSEESKESSTTLSSPVLHNGTSGEKQSIFRITRSKIYQQKQIASQGDADSQRTTRKRIWERKTDTSNEHYETLSNRFCRYRKIPRRSATVAANKLRMMSDVEEEFFSSESGGTRNKNRKRPHRNASAAARKLLLNDSEEESSMHSESDKELEDQSDRRKAPKTEHFAVKKRHSSGSESGNSNSESGMKKTPKRYSVKDYKKRLRTVCRVSPKMKHIPECSVDDSKHHISDDESVQKTVHQLSSSYEINANSSSETDSEQECKVLPRHAFTRASFRFRKGKILSDSEVESGRDDETPSSSKIRIFSETSKLGQNDSSNLESGTDSSHSRKSQGRKGIRKDSVAKANGLSKSTRKRPFQSDCQTGACKSEYIYGKLPLRSDTVTVTVNKLSLANDEEDFTNSETTRTYSDNNRKLNCRSIVSATRKQLDSSADGSQESGEAKRKQYCRRKCIQSSTAAKIDGSDSENSSYSESAERKKMHIKKAHMHKPRTPYDRSINPILEKSLKSHLSKDRNPDKHPIFTHNTIVTSDLEGDSELNQYNDNIKEFQKTVDSSGKSKNESDIQHKGNGTDSLPNSKESSKTPKSKTRVDSSCQSDLEIKSCNNASNYSVIKTQPKKKKEKTEEGRIKEESASQLLNQSPIVLRNRNIYKSCTELNNMSMTIKALNGKKIPRRSAAVAASKIKLISDAKEEFSGSEVGYCRKKNRIRPLRSASVAARKVLDDLPSLSDSGTELKGHKIKGRNNQTCSYSPRQSNKILKTRCLDAEEESVLKQRRSHSNGSRQVSIKSEYKKITVKHFDDSSSSITSEEQKSSSQDNVTSDSAADSELEKEYNFTNVRNTRHNGERDNMEQSALSKNKQLGIPESSKSEPDRSSHFSSDSETQTESKPINNNGGVKTTKRKRKATPANKNGFSESSRIPQRRKRPKVNEENDSDELHYTKYKPINRRSKVKTRNRGRRTVRYADDEEDCET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDF9</accession>
    <name>A0A6I9XDF9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras-related protein Rab-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RAB3B</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Lipid-anchor</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911902.1">
        <property type="nucleotide sequence ID" value="XM_014056427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDF9"/>
    <dbReference type="GeneID" id="106541092"/>
    <dbReference type="KEGG" id="tsr:106541092"/>
    <dbReference type="CTD" id="5865"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01865">
        <property type="entry name" value="Rab3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000206">
        <property type="entry name" value="Ras-related protein Rab-3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037872">
        <property type="entry name" value="Rab3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013911902.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="25147" checksum="E3CFC7C1FDAA8519" modified="2020-10-07" version="1">MASVTDARYRPKDVSDQNFDYMFKLLIIGNSSVGKTSFLFRYADDTFTPAFVSTVGIDFKVKTVYRNDKRVKLQIWDTAGQERYRTITTAYYRGAMGFILMYDVTCEESFNAIQDWATQIKTYSWDNAQVILVGNKCDMEDERIIPIEKGKHLAEQLGFDYFEASAKENINVKQVFERLVDIICEKMSESMESEASKATAGKNVRLTYQPPPLQQKCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDW3</accession>
    <name>A0A6I9XDW3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">lysoplasmalogenase</fullName>
            <ecNumber evidence="6">3.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMEM86A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = a 2,3-saturated aldehyde + sn-glycerol 3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:22544"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = a 2,3-saturated aldehyde + sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:16905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
            <dbReference type="ChEBI" id="CHEBI:143890"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM86 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013912396.1">
        <property type="nucleotide sequence ID" value="XM_014056921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDW3"/>
    <dbReference type="GeneID" id="106541458"/>
    <dbReference type="KEGG" id="tsr:106541458"/>
    <dbReference type="CTD" id="144110"/>
    <dbReference type="OrthoDB" id="2133758at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047408">
        <property type="term" value="F:alkenylglycerophosphocholine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012506">
        <property type="entry name" value="TMEM86B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885">
        <property type="entry name" value="GH04784P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885:SF9">
        <property type="entry name" value="LYSOPLASMALOGENASE-LIKE PROTEIN TMEM86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07947">
        <property type="entry name" value="YhhN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912396.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="26670" checksum="28AA1F5B6F8FB3FA" modified="2020-10-07" version="1">MVSPVTVVKSEGPKLVPFFKATCVYFVLWLPTSSPSWFSALIKCLPIFCLWVFLLAHGINFLTAHHNACRIFAGLIFSALGDAFLIWQEQGYFIHGLLMFAITHILYSSAFGIKPWDLKVGLGFGLVSSFCYTFLYFYLSGPFTYLVAVYVALIGFMGWRAMAGVQLCNDLWTWTKLSACIGSVLFMVSDLTIAVNKFCFPVPYSRVIIMATYYAAQMLIALSAVENKDEEVFKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFJ3</accession>
    <name>A0A6I9XFJ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc finger and SCAN domain-containing protein 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106541662</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912647.1">
        <property type="nucleotide sequence ID" value="XM_014057172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFJ3"/>
    <dbReference type="GeneID" id="106541662"/>
    <dbReference type="KEGG" id="tsr:106541662"/>
    <dbReference type="OrthoDB" id="9048260at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="124"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912647.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="37607" checksum="40D04201EB4DFDBB" modified="2020-10-07" version="1">MSEQAGWGAFERNKRAPFVGMEERWESQWQQFLQTLQPVHPGEGKSKMSDASPWEDPKVFLASFEQVAQACRWPREEWVSCLLPALSGEAEEAFQKLEIGERVNYGKVKAAILKGEAVKREDQRQHFRQFCCQEVEDPWMVYKQIQELCRQWLKPERRPKEQILELLILEQFLASLPPKLQSWVQPRRPDTCSQAVALVEDFLRSQQGIKSEACQGPPNEEPTNFECAEQEPLEAVKRENVEVEIGMLGPGIESPDPPSSMLPSERLGMVQTALKEAVTDLKERDVHLPTAKWDLVQPVPKRMAPEALQSEHRNIGSLNSSLERPDSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFM3</accession>
    <name>A0A6I9XFM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger and BTB domain-containing protein 49</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Zinc finger protein 509</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ZBTB49</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transcription factor. Inhibits cell proliferation by activating either CDKN1A/p21 transcription or RB1 transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912971.1">
        <property type="nucleotide sequence ID" value="XM_014057496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFM3"/>
    <dbReference type="GeneID" id="106541919"/>
    <dbReference type="KEGG" id="tsr:106541919"/>
    <dbReference type="CTD" id="166793"/>
    <dbReference type="OrthoDB" id="407106at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18233">
        <property type="entry name" value="BTB_POZ_ZBTB49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001818">
        <property type="entry name" value="GDNF-inducible zinc finger protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000166">
        <property type="entry name" value="Zinc finger and BTB domain-containing 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000835">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000927">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001099">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001123">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF30">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="20">
        <location>
            <begin position="25"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="311"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="617"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="708"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013912971.1"/>
        </source>
    </evidence>
    <sequence length="722" mass="78458" checksum="5734B03227451654" modified="2020-10-07" version="1">MDAFASHSCHLLQQLHNQRIQGLLCDCMLVVKGVSFKAHKNVLAAFSQYFRTLFQNSPSQKNDVFHLDIKNVGGIGQILDYMYTSHLDLNQDNIQNLLDVARCLQVQNILNLCHSFLKTSRSVEQPASLTSNGAFSLQNALAADTHCGHWDGLHLCTTAACKALDDQPPGSQPCAPHSSSAEGAKPKQGPVGPGRADLSFKLPNGHYKLRNFYSKQFCKETIRPSSEGLLEVPPDFAISPAQDAAQEATLGSLAQCALEPSDCAPLPPPFLAQPLHGCAEDQHAEGASVQPAKPMRPKKAIHLQRLNLLQSQRSPEPPSAGDPDGAAAKGNESGSRSSTKRTATVEVPKGKHPSGILSSECVELEPSREPPGPESPFDVSLPERQYPCALCGKVFKHPSNLELHLRSHTGEKPFECNICGKCFSQAGNLQTHLRRHSGEKPFICEICGKRFAASGDVQRHIIIHSGEKPHLCDICGRGFSNFSNLKEHKKNHAAEKVFTCDECGKSFNLPRKLVKHRIRHTGEKPYNCSVCGKCFAGSGDLRRHIRTHTGEKPYPCETCNKCFSRSAVLRRHKKTHCKADCESAAIAEEFSHAMEIPDLEKSQCSDFFTQEISASFLSPSEKRPPHSGESPNEFGPPGAPFGRARPGVGKDAQEPSLDPAKACKSPEAPIPSCTFKGSHVSSEEGPLQSGPLPGVRSSAPLQVRGSCQAPNQRGAFSSMTLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFT9</accession>
    <name>A0A6I9XFT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Intraflagellar transport protein 81 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">IFT81</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the IFT81 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914529.1">
        <property type="nucleotide sequence ID" value="XM_014059054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFT9"/>
    <dbReference type="GeneID" id="106543138"/>
    <dbReference type="KEGG" id="tsr:106543138"/>
    <dbReference type="CTD" id="28981"/>
    <dbReference type="OrthoDB" id="276029at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.70">
        <property type="entry name" value="Intraflagellar transport protein 81, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029600">
        <property type="entry name" value="IFT81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041146">
        <property type="entry name" value="IFT81_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043016">
        <property type="entry name" value="IFT81_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15614">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 81 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15614:SF2">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 81 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18383">
        <property type="entry name" value="IFT81_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IFT81 calponin homology" evidence="8">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="73"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914529.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="30474" checksum="25F6F45A42F2AB72" modified="2020-10-07" version="1">MLTLLGILKYKPPGGTSDLSTFRQGLVTGSKPVIHPVLHWLLQRTTELKKRAYLARFLIKIDVPTEFLQDDTVADTNRQYEDLMEAFKSLHKESEQLKTSGFSTAEIRRDITAMEEEKDQLMKRVERLRKRVETVQSHQRMLEMARQLRVEKEREENLAHQKQEQKNQLFHAEQRLHRVQLQLKDMRHAAVDSKPESLMKSLEEETKFNTYLVSEKFPRELETKKQSLLLLQKVVAEPAMGQSDLNELEAKVSGQEDKWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGP8</accession>
    <name>A0A6I9XGP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Zinc finger homeobox protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">AT motif-binding factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">AT-binding transcription factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Alpha-fetoprotein enhancer-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Zinc finger homeodomain protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">LOC106542169</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 25 26">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913296.1">
        <property type="nucleotide sequence ID" value="XM_014057821.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542169"/>
    <dbReference type="KEGG" id="tsr:106542169"/>
    <dbReference type="OrthoDB" id="6417226at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013913296.1"/>
        </source>
    </evidence>
    <sequence length="3578" mass="387125" checksum="86E33C9A5016F2C9" modified="2020-10-07" version="1">MEGCDLPVILGKDLGGAGPQHQQWPEFGSAHHLPTTPDDTESTAAAQLGNERASFCERLPGSAAAAAACSPEPSSKEISCNDFAASFPSLQAYMEPPCASAHPLLPSQEDCGSDTGEEEEEEEEEESDIENLAGEIVYQPDGSAYIVESLSQLSQGGGGLPPLLANSALAIPGAKSGDSAAAVPQVYPQIINTFHIASSFGKWFEGPDEAFPGTSALVGVSPVLHSFRVFDVRHRSNRDYLNSDGSAKSSCVSKDVPNNVDMSKFDSFVLYGKRKPILMCFLCKLSFGFVRSLVSHAVHDHRMTLSEEERRILSNKNISAIIQGIGKDKEPLVSFLEPKNKNVSHPLVSATDLISPGHSFYGKFSGIHMEGEGGAQTGAASTSDQPQAADLLAPGGLLNLGGLTSSALKTPITSVPLDAPSSSPSAASSEGKGPGGPGSDAQGSSLAKAELPGEDQEEEEEEEEEEGAGGGGQGLFPHRLEQDPEEKLPATAEPGLAAHSGSRLKDLALSNQSISPLMPHVLQGVSRGLAPSAAPPPAPPFVFDGANRRSCLSFNREGSQRAADDTGEESANRDDAPELRESPEGEDGLCLPHHQHGGPLCDLGGEGCPSGGGVECPKCDTVLGSSRSLGGHMTMMHSRNSCKTLKCPKCNWHYKYQQTLEAHMKEKHPEPGGTCAFCQSGQLHPRLARGESYTCGYKPFRCEVCNYSTTTKGNLSIHMQSDKHLNNMQTLQNGSGEQGFSHAAGAVAVATAAATSIGGGCGAPSPTKPKSKPSWRCEVCDYETNVARNLRIHMTSEKHMHNMVLFQQKSSQVPLGLDGLSAEAELYNYYLGQNLNMDHSPASEAQFLTGSHFQVEPSHTLASVAPSLVGGETPLEPPLGGGQLVPEELVNLGETVDQSSDPSRKLFQCAMCNKFTTDALDLLGLHLNAERALPEEEWKGAVGEAYQCRLCHYSTQLKANFQLHCKTEKHLQKYQLVAHIREGGKANEWRLKCMVMGNPLHLKCNACDYYTNSLEKLRLHTGNYRHEASLKLYKHLQKHESRLEGDSCFYHCVLCNYSTKAKLNLVQHVRSLKHQRTESLHKLKRLQKSLPEEEEDLGQIFTIQHCPAADIEELIGDAEGLGEVATSPDEAAKEVESVADQDQATWAAEGPQAEKEPAEPSLPSKHPSFSRHSASPVPRWPTPSEEAKLEQVYHCLYCKYSNPDVNRLRVHVVTQHSIQPMLRCPLCQDLLSNKLHLKLHLTHLHSVSPDCVEKLLLTVTTPEAVMPSNVFPTAAAAAADRDGNEEPLQPPESSEEGGKGLRLLPEAVRHGGSSGGKPAASEQPGAGEEETGFFCWKKGCGQGFRSSSALQAHFGETHGRRPQLPVSDRHVYKYRCNQCSLAFKTVEKLQLHSQYHAIRAATMCCLCQRSFRTFQALRKHLEASHLDLSEADIQQLYSGLLVNGELLAVGDLPLAEDHPVMTTEEEEKEEENSDQEDKQSPAGSDCGSLQEDSGSEPKRALPFRKGPNFTLEKFLDPARPYKCTVCKESFTQKNILLVHYNSVSHLHKLKRALQESSASQSEATSSPDHKPFKCSICSVAYSQSSTLEIHMRSVLHQTKARAAKLEAVSGSGHSSASLGSSMPSPLGAGTTSLTSSSSSAAPQSNISTGSTGSTLPLLSQASCDPTGQLPLNLPIRSSHSPAAPLSPSEPKETNRKKPGDAPACRQPLPPPPNSLMQAQLQQELQQQASLLQAQLFSPALLPHFPMVPEALLQQQQQQQLLFPFYFPSAELQLNAEVSLPMTNGTLTLGLLEDLKPQLPQPSHPQMQQPPLPLSQPPCVPVPPSVAPDKKAKSVAKEKEAQREQEMAEKGGRSPSCQEPLPEASKAQEKADPAGPTEAAGSSPEEAPWLPPRIAVDARGNATKALLENFGFELVIQYNENKQKAAGRVEQGIGLERLECESCSKFFSNVLILKTHQEHVHQHYLPFQQLESFAQQYREHYDQLYPLRSLTPEPAPPPPPAPAVTAAPPQPPSTPTLQVSASPMTSPSLAPNQPPLPHTQPLPLPMELPLFSPLMMPTMPLQALPAQLPPQLAPVDSLPAELAQLYQHQLNQGLLQQQQQQQQQQQNKRPRTRITDEQLRVLRQYFDINNSPSEEQIKEMAEKSGLPQKVIKHWFRNTLFKERQRNKDSPYNFSNPPITSLEDLKVDSRPSSPEPQKHEYWGSKRSSRTRFTDYQLRVLQDFFDANAYPKDDEFEQLSNLLNLPTRVIVVWFQNARQKARKNYENQGEGKEGERRELTNDRYIRTSNLNYQCKKCSLVFQRIFDLIKHQKKLCYKDEDEDGQEDSQNEDSMDATELLTPSSSSCSTPMPSQAYGTPTSSASAAFPSQSTTEADSDCPAACQPKANAADEKPKHPEPSSAAQQSPASDKQRQPKRDGQVPAQDQEELKLSSVHPKASPPLQQQPAHPLQGGPPQASPPPAQLSHLPHKPLATPIAQQLASLPPQLVPYQCKQCKLAFPSFEHWQEHQQLHFLSTQGQFLHPQFDLPFMLFDPSNPLLASQLLSGTLSQLPMSSATSPSTPTATMSSLKRKLEEKARVSPVGNDGGAGGEEPQRDKRLRTTITPEQLEVLYQKYLLDSNPTRKMLDHIAHEVGLKKRVVQVWFQNTRARERKGQFRAVGPAQAHRRCPFCRALFKAKTALEAHIRSRHWHEAKRAGYNLALSAMLLDCEGGGGGVGGLLGKGDLFDGANFAQLSAPGSSSDSQGIPLSPGSKSLELSPQTLLSPSSIKVEGVEDLESPSVSSVHLSFDQAKLDNDDCSSVNTALTDTTTGDETNADNDSATGVFTEARGSLGPSEGLTKAAMLAMSEYEDRLSSGLVSPAPSFHSKEYDNEGTVDYSETSSLADPCSPSPGTSGSAGKSGDGGDRPGQKRFRTQMTNLQLKVLKACFSDYRTPTMLECEVLGNDIGLPKRVIQVWFQNARAKEKKAKLNMAKHFGINQSGYEGPKTECTLCGIKYGARLSVRDHIFSEQHISKVKETVGSQLDKEKEYFDPATVRQLMAQQELDRLKKANEALGLAAQQQGMFDSPPLQALNIPASYPAALQSIPPVLLPGLNSPSLPGFTPSNTALTSPKPALMGLPGTSVPSPGLPTSGSPNKTAPLSSPPPAQTSVGSTTPLPLRKDKESEKAKEKERPSKTRGEALSGPKKDKGEAPNPGPAFPAMGPALEYSAEPTQLQALQAALNTESLALLTNPFLPCFVPGFSPYYAPQLPGALQSGYLQPMYSMEGVFPYGPALSQALLGVSPGSLLQHYQQYQQSLQEALQRQVQLPLPKGSQPPVPTEGLSPEKGPAKESPRPEEKSSSRQGSPRRELATAGSSSSSSSAPDSLWDPFIVPPVQYRLVCRKCQAGFGSQEAADVHLKSLCCFGQSMGTLPEMALQVLAGGGGSYQCVACESTVCGEEALQQHLESAPHRQHQTVPRAAARNAKEPPSQLPHSACLPDPSTASTSQPTAHPKNGPLPAAAPHSSKKPWPPAASPPGKPPPLSSSSTVTSSSCSTSGVQPSLMPRKFSSEESDSGRGPEAGAPLEPSSPRDGGLPSVDPFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH96</accession>
    <name>A0A6I9XH96_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nudix hydrolase 20, chloroplastic-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915084.1">
        <property type="nucleotide sequence ID" value="XM_014059609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH96"/>
    <dbReference type="GeneID" id="106543562"/>
    <dbReference type="KEGG" id="tsr:106543562"/>
    <dbReference type="OrthoDB" id="10261522at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="136"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915084.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="34024" checksum="777C87804A26DF17" modified="2020-10-07" version="1">MAAEGWSERVRKLLKKMNSFHGAGSSKALCLPFMVAGQQVGYVPPAVAKCLHQYPTVFSVSQGDKASPRVELNKQLTSCDQRTAAVQRVLKELKAQQAFPCLKGWRDEMYNVMPYFCDTPFFSMERAATPLLGVKTYGAHLNGYTWRNDEMHMWLARRALNKPTYPGLLDNLAAGGISSELGVRETLIKECQEEACIPASLATLSKSVGTISYTYEGSDGGINPECQFVYDLELPEEFVPQVGDGEVQEFYLWSLDKVREAIGSSDFKPNCAMVALDFLIRHSYIQPDQGRPPQCQLCMVKVKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHE2</accession>
    <name>A0A6I9XHE2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein C1orf87-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106542339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913516.1">
        <property type="nucleotide sequence ID" value="XM_014058041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHE2"/>
    <dbReference type="GeneID" id="106542339"/>
    <dbReference type="KEGG" id="tsr:106542339"/>
    <dbReference type="OrthoDB" id="9989690at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040774">
        <property type="entry name" value="DUF5580"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049247">
        <property type="entry name" value="DUF5580_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34830:SF1">
        <property type="entry name" value="GENE 12695-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34830">
        <property type="entry name" value="SIMILAR TO HYPOTHETICAL PROTEIN MGC34837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20743">
        <property type="entry name" value="DUF5580_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5580" evidence="2">
        <location>
            <begin position="61"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913516.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="16030" checksum="C6345ECA1623F5AF" modified="2020-10-07" version="1">TSSGGSGRHHHPRRRFCEHAHERTGRTEEKTEDNSQIKEQYKLQTANPWNLYDASDSEDQEAWIDRFRKMEKALYLSDVKNTGKLEKEKAKRLIHNYNQIYDLCLSPLKIDKAFRPFHYGQDLPLQPLLQYLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIE2</accession>
    <name>A0A6I9XIE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flotillin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FLOT2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Heterooligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913687.1">
        <property type="nucleotide sequence ID" value="XM_014058212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIE2"/>
    <dbReference type="GeneID" id="106542472"/>
    <dbReference type="KEGG" id="tsr:106542472"/>
    <dbReference type="CTD" id="2319"/>
    <dbReference type="OrthoDB" id="6080404at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016600">
        <property type="term" value="C:flotillin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045661">
        <property type="term" value="P:regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03399">
        <property type="entry name" value="SPFH_flotillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000003">
        <property type="entry name" value="Flotillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031905">
        <property type="entry name" value="Flotillin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806:SF46">
        <property type="entry name" value="FLOTILLIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806">
        <property type="entry name" value="FLOTILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15975">
        <property type="entry name" value="Flot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0967">Endosome</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="5">
        <location>
            <begin position="38"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013913687.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="41810" checksum="4A99D02793690DC3" modified="2020-10-07" version="1">MTILCRCENIETSEGVPLYVTGVAQVKIMTDKELLAVACEQFLGKNVQDIKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEILSFTIKDVYDKVSYLSSLGKTQIAVVQRDADIGVAEAERDAGIREAECKKEMLDVKFMADTKVADSKRAFEMQKAAFTQEINIKSAQAQLAYELQGAKEQQKIRQEEIEIEVVQRRRQIDVEEKEVLRMDKELIATIRLPAEAEAHRMQEIAEGEKVKQVLIAQAEGEKIRKIGEAEALVIEAIGKAEAEKMKLKAEAYQQYGEAAKMALVLDALPHIAAKVSAPLAKVGEIVILSGDNNKVTSEVNRLLAEIPASVHALTGVDLAKIPLIQKATGVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK44</accession>
    <name>A0A6I9XK44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">Thromboxane-A synthase</fullName>
            <ecNumber evidence="7">1.14.14.1</ecNumber>
            <ecNumber evidence="8">4.2.1.152</ecNumber>
            <ecNumber evidence="30">5.3.99.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">Cytochrome P450 5A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Hydroperoxy icosatetraenoate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="38" type="primary">TBXAS1</name>
    </gene>
    <organism evidence="37 38">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="38" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="38">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="33">Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation. Also cleaves PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2. Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + AH2 = (15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:48856"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:57446"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:48857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:48636"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57410"/>
            <dbReference type="ChEBI" id="CHEBI:57446"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:48637"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a hydroperoxyeicosatetraenoate = an oxoeicosatetraenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:55556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59720"/>
            <dbReference type="ChEBI" id="CHEBI:131859"/>
            <dbReference type="EC" id="4.2.1.152"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:55557"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>prostaglandin H2 = (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoate + malonaldehyde</text>
            <dbReference type="Rhea" id="RHEA:48644"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:90694"/>
            <dbReference type="ChEBI" id="CHEBI:566274"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>prostaglandin H2 = thromboxane A2</text>
            <dbReference type="Rhea" id="RHEA:17137"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:57445"/>
            <dbReference type="EC" id="5.3.99.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:17138"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 34">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 35">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="7"/>
    <dbReference type="EC" id="4.2.1.152" evidence="8"/>
    <dbReference type="EC" id="5.3.99.5" evidence="30"/>
    <dbReference type="RefSeq" id="XP_013914376.1">
        <property type="nucleotide sequence ID" value="XM_014058901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK44"/>
    <dbReference type="GeneID" id="106543013"/>
    <dbReference type="KEGG" id="tsr:106543013"/>
    <dbReference type="CTD" id="6916"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106256">
        <property type="term" value="F:hydroperoxy icosatetraenoate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004796">
        <property type="term" value="F:thromboxane-A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000182">
        <property type="entry name" value="Cytochrome P450 3A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050705">
        <property type="entry name" value="Cytochrome_P450_3A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24302:SF47">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24302">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="23" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12 34 35" id="KW-0349">Heme</keyword>
    <keyword evidence="19 34 35" id="KW-0408">Iron</keyword>
    <keyword evidence="24" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="21" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="25" id="KW-0456">Lyase</keyword>
    <keyword evidence="22 36" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 34 35" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20 35" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="18 35" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="9" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 36" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 36" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="34">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00033404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00036380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00036424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00036475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00038872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00040834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00042726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00054825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="RefSeq" id="XP_013914376.1"/>
        </source>
    </evidence>
    <sequence length="493" mass="56744" checksum="31A6EC934CEFB21B" modified="2020-10-07" version="1">MGLTDGLRLDLVETNVTTATLTFLVIVLVLLAWHITSSFSRLAKVGVRHPPPLPFIGNLLYFHEGFWESHNKLISNYGPSCGYYIGRRMYIVISEPDMIKCILEDDFRNFRNRMRPDLVFKPFADSIVMLRDEKWEKVRSILTPAFNIAKITEMTPLIDEACNILLNNLKVSAESGTAFDIQRLIIEQLLMLLFQNSIKVAFPYIMIPLLRILPNKKRDEVNGFFISLIKNMITLRDQQDPNERRRDFLQLMLDARIPTTDIVMDQSDKVSQDDFPEALPKKQQMWLTDDEIAGQASLFLIAGYETSNSTLSFGSYLLATNPQCQEKLLQEVDDFFSKHDIPNYENIQELPYLDMVIAETLRMYPPAFRFTREAAKDCWVLQQKVPAGAIIEIAVGHLHYNPKYWPEPEKFIPERFMSEAMQQQHPFAYLPFGAGPRGCIGMKLALMTIKIALLRILQRFMFKTCPETQIPLQVKSHSTLGPQDGIFIKIVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKG7</accession>
    <name>A0A6I9XKG7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EMILIN-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Elastin microfibril interface-located protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">EMILIN2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Homotrimer associated through a moderately stable interaction of the C-terminal globular C1q domains, allowing the nucleation of the triple helix and then a further quaternary assembly to higher-order polymers via intermolecular disulfide bonds. Interacts with EMILIN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916289.1">
        <property type="nucleotide sequence ID" value="XM_014060814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKG7"/>
    <dbReference type="GeneID" id="106544519"/>
    <dbReference type="KEGG" id="tsr:106544519"/>
    <dbReference type="CTD" id="84034"/>
    <dbReference type="OrthoDB" id="9944757at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000011">
        <property type="entry name" value="Elastin microfibril interfacer 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF5">
        <property type="entry name" value="EMILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07546">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EMI" evidence="16">
        <location>
            <begin position="15"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="15">
        <location>
            <begin position="914"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="174"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="751"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="864"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013916289.1"/>
        </source>
    </evidence>
    <sequence length="1071" mass="121017" checksum="BCB5370DEC18E21D" modified="2020-10-07" version="1">MRGLDDKTTVAGIGNWCAYVVNKNVSCSVLGGTESFVEVQYICLWNQEPCPHVPMYRTSLRPKYILTYKMVTELEWRCCPGYMGVDCKEAVAEKPRSIAYPAELPPNDMKPRLDSRPKQKQEDSQDKKIQFLEDELFRLTQTVLELQSAITNVDKNPKVTVQEDASQREISWLNNLHDHPGLDSTSEEKTENKYPLNTNDSYKPETERLTSEEDKIEDGLKAKINRLEQLNGKVNDYEGQLKQLQEAAQGPTITMPNSQLYQEYIDAKFEALRQDMLEGFEKKMADVKNSCEYKLTDIQQYCNDHEANFLGLRESVAEKENDLRKQIQNLQNVIEVPLNHSCCSDDIREQISTLDKKIEKIAEANKILNARIDNEIKHISTPYSEDNFDEKWEEIEARINVTEKNAEEHCFYIEETLRNVIATKTDEVRDLFDKKFQVIESKLGSTILDIANGTSLDDIYITQGSTLQNNSESENKRIVLEINLMKNKLQHTEEVCQFLSQYLESLQKGIENTNSKYDQLFLKTKDNSDILRSFNSSLSEKFSLIKGTQRDIHKLHKDLRIVRFGLHAIDKDVKLLQNDLNGCKEQLLGINSTCGKTQLELLRKTDEIQKMMNNQTIHQNNNCCNHLNKKINQLNREELFNLTKCKDKIPGISDLEDKVSNVEKTCNKLDSVSSSLQKIKEGVNRHISSLWNCFDQMNITVISHSKDILSLKNFTQQFNSQFHKFAIDLQNPKKIQPSIYEKPSVLLAKEQEKEQGEIPQPHSDPTKPLQSSQAGKFPAPSQPRIPAQSQTEVHLHPSRVQTPVQIPELRIPLQPSRPQIIIQPPLQGIPLQSSDSRIPMEPSKPGVSSLPGSSGLLMEAGQAGPPGTLLRSRKGLPKRINGKDGQNNMLISEGYAGAPGYPNPPKSPSPQVGAFTSLVSFSAGLTKKPFPEDVGVIHFNKVLVNDGNYYNPNTGFFTTPYEGRYLITAVLVPERGEYIEAMLSVSNSSVAQLHTAGYKKDQSEHRKPRSGKRICGGTGAFQLVLHLKTGAEVGVVVTGGKLAYADSDEIYSTFSGVFLYPEQKIKPELNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKW1</accession>
    <name>A0A6I9XKW1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C3orf18-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914587.1">
        <property type="nucleotide sequence ID" value="XM_014059112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKW1"/>
    <dbReference type="GeneID" id="106543189"/>
    <dbReference type="KEGG" id="tsr:106543189"/>
    <dbReference type="OrthoDB" id="6381603at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043239">
        <property type="entry name" value="SMIM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47730">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47730:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914587.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="11775" checksum="B74F4A2E1F656016" modified="2020-10-07" version="1">MNNTTMPTSAGPTNDSLVGYILVPFFLITIVGIIMAVMMYIQKKRRYDRLRHHLLPMYSYDPAEELHEAEQELLVEPEDTKMMRGWGGYQPYRNSFMDVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLE7</accession>
    <name>A0A6I9XLE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">LOC106543329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914791.1">
        <property type="nucleotide sequence ID" value="XM_014059316.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914792.1">
        <property type="nucleotide sequence ID" value="XM_014059317.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914793.1">
        <property type="nucleotide sequence ID" value="XM_014059318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLE7"/>
    <dbReference type="GeneID" id="106543329"/>
    <dbReference type="KEGG" id="tsr:106543329"/>
    <dbReference type="OrthoDB" id="2016523at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF11">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN MGAT4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MGAT4 conserved region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914793.1"/>
        </source>
    </evidence>
    <sequence length="465" mass="53889" checksum="803B3AD2A9466425" modified="2020-10-07" version="1">MRCSLKRSLATAFSLCVFLLIMYLASWHHEDLVMNDVQKWAQTVGLHPIATDWTVSSLRMFINATNTLNISYDYLVGSPPLQKKFLTLGLCSVKRMRQDYLLNTLTSIFEQSSEEEMKEMVVVVHLTDPDHKWNSQMALEIARSFPNQLHQRNLVVIHTRLEYYPPLEGLKRNFNDPEDRVRFRSKQNVDYAYLMNFASNLSTYYLMIEDDVRCTKHFLTVIKRNLASQEGTSWVTLEFSKLGYIAKLYRSTDLPRLTRFLMLFYQEMPCDWLFVHFRLLLTQKEEIRFRQSLFQHVGHYSSFQGTLNKLEDEDFEEDFSKIPDNPPADIITDIATFRNSIPSKAYSSVIGYFWGKAPSVGNSFTLIFKKAAPIFRIQIHTGSKEFPESYLHEGLVALGTNKLQTLRPRCANYITIGHFTKGIFDKQNLENSITSDTVCVQILVTNTQSESLIISNISIWIKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM59</accession>
    <name>A0A6I9XM59_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Rho-related GTP-binding protein RhoU-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106541105</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911918.1">
        <property type="nucleotide sequence ID" value="XM_014056443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM59"/>
    <dbReference type="GeneID" id="106541105"/>
    <dbReference type="KEGG" id="tsr:106541105"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000561">
        <property type="entry name" value="rho-related GTP-binding protein RhoV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911918.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="24873" checksum="EEF264E926568BE6" modified="2020-10-07" version="1">MQLPPVPPHRPRARRRRPLRALECVLLGDGAVGKTSLALSYSTNGFPTRYVPTALDRFSAVVQVDDAPIRLQLCDTAGQEGFDALWQACCPKADICLLCFSVVAPTSFRNIVDKWHPEVCRHCPSTPVLLVGTQADLRQDVKVLIALSQRQEKPVPPTAGRSLARKLGMAGYVECSALTQQNLKEVFDTAIVLGLRAGEGPKGQRAPPSCIWALSKDWWNKYVCVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMG6</accession>
    <name>A0A6I9XMG6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein FAM89A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FAM89A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM89 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915116.1">
        <property type="nucleotide sequence ID" value="XM_014059641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMG6"/>
    <dbReference type="GeneID" id="106543589"/>
    <dbReference type="KEGG" id="tsr:106543589"/>
    <dbReference type="CTD" id="375061"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46949">
        <property type="entry name" value="LEUCINE REPEAT ADAPTER PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46949:SF3">
        <property type="entry name" value="PROTEIN FAM89A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00049748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013915116.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="15617" checksum="A65B6D8592BCF65F" modified="2020-10-07" version="1">MSASGLPWSIDSGGSGLPPLPKSLSGLLNSSSSGGAPGGAGGRWRDLERLYTQKSRIQDELSRRGSGGGGGGGRGSPRPPKPPNLDAALALLRKEMVGLRQLDMSLLCQLYSLYESIQEYKGACQAVSSADCAYALENGFFDEEEEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNW7</accession>
    <name>A0A6I9XNW7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nck-associated protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NCKAP1L</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912433.1">
        <property type="nucleotide sequence ID" value="XM_014056958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNW7"/>
    <dbReference type="GeneID" id="106541487"/>
    <dbReference type="KEGG" id="tsr:106541487"/>
    <dbReference type="CTD" id="3071"/>
    <dbReference type="OrthoDB" id="548214at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019137">
        <property type="entry name" value="Nck-associated_protein-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12093">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12093:SF9">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09735">
        <property type="entry name" value="Nckap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912433.1"/>
        </source>
    </evidence>
    <sequence length="974" mass="111869" checksum="8299CEE84DCE03BB" modified="2020-10-07" version="1">MSLSSVYEHKFAEKLTILNDRGKGVLIRIYNIKKTCTDSQAKLPFLMEKTMESSIKYINKKFPNLDTRSSTQHLGPVHKEKADIFRALTPYYQTFVDVMEFRDHVYELLNTIDASQCYLNINVNYDFTKNYLDLIVTYVSVIVLLSRIDDRKVLIGLHHCTHEMIHGTSDASFRRLGQMMIEYENPLRKLLEEFGPHTKTVSHALLSLHFLFVRRNHSADQWRGDQLFSLISNPASMLSPASSDTMACEYLSWEVLERWIVMGFLLCHSCLGSTQNCLELWRMALRGSLYITLIRDEVLYIHKVTEEAFSGIKGYGKRITDIKDCKEHALQQSGQFHRGRRNYLRNAVREMETILANEPGLLGPKAIYVFMALSFCRDEVAWLCRHSEHKGKNQEEFTDSCLAELLFLMEKLRNLLKQYQNVIQRYHVQYLSRFDAQVLGNVIQDLTVCPEEESVIMSAFVNTLTSLTVKQVDVKEKFEFKGLRLDWFRLQAYTSVTKAPLQLRENHDLAKVMNLIVFHTKMLDFVEELIVETSDLSFFGFHVRHLEKLFAASLEEPSMLRYVIIFPLLCADFSNTIHPMCPEEYPHLRTCALAICNNFLEEIAKQTSTCILDACAEQCNLNEQLLPKHCAPTISKARTKRVQKKTSKKLEPERDKPGAESIRKDRSLVTNMCNRNQKMRALAELLGPYGMKFLSDNLMWHVTSQMVELKKLVTENMDILVQIRSNYCHPEQMATLMPRLSGIDNVLKRMTIIGEILWFRSMAQEGLQGVFTNRCPFLMGPIQYLKEFVNPEMDIKVTLSIFELATAAGLPCDIDPALVSALINLKKDSSSPEEDYKTACLLLVFIAVSLPLLVSDPSSIYLTEIDGYRNNIHCLAKAIIQVSAALFTIYNKNIESHLKEFLVLASASISQGGLDVDRMKAKNWESISLLLYLLVEESSFLTVDMLETCFPYVLLRNAYREISRNALLSRLSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP27</accession>
    <name>A0A6I9XP27_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Calcium-activated potassium channel subunit alpha-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106544010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915621.1">
        <property type="nucleotide sequence ID" value="XM_014060146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP27"/>
    <dbReference type="GeneID" id="106544010"/>
    <dbReference type="KEGG" id="tsr:106544010"/>
    <dbReference type="OrthoDB" id="10035564at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ca2+-activated K+ channel Slowpoke-like C-terminal" evidence="1">
        <location>
            <begin position="39"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915621.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15290" checksum="A3503D2FF7045E9F" modified="2020-10-07" version="1">MIRDLLIDCKNQKSGAATCKVLLVSQKNRKKHTYHNYHVLALLQTLVTGGTSPDLEEYLEEGSSLTSNHSNALFLGLRNRCKLALLTLVDDPELMEGLRLFFGDVFIKALDMYGILCLGIYRLKPHPNPYEIRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPN6</accession>
    <name>A0A6I9XPN6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone-lysine N-methyltransferase, H3 lysine-79 specific-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912678.1">
        <property type="nucleotide sequence ID" value="XM_014057203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPN6"/>
    <dbReference type="GeneID" id="106541684"/>
    <dbReference type="KEGG" id="tsr:106541684"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912678.1"/>
        </source>
    </evidence>
    <sequence length="61" mass="7811" checksum="8BCF791659566FE1" modified="2020-10-07" version="1">MFIWLMVFLETDYLKRKKERKKGRRREGGRKERERKKERKKEKNYPERQDKSRSKLKTIWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQE5</accession>
    <name>A0A6I9XQE5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LOW QUALITY PROTEIN: apolipoprotein L3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106544294</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916011.1">
        <property type="nucleotide sequence ID" value="XM_014060536.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106544294"/>
    <dbReference type="KEGG" id="tsr:106544294"/>
    <dbReference type="OrthoDB" id="6363454at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF27">
        <property type="entry name" value="APOLIPOPROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05461">
        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916011.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="36158" checksum="49C48AF214A965C3" modified="2020-10-07" version="1">MHLRRSAKAFKLHHSSEIPATPREASQCILEGHHLRELERKERKVPWEETAREYFVKEFPTQKREVEKCFSCPQEVANHIDRIHKGCTTASLVSCSINATSGILTILGLTLAPISAGGSLILTATGTGLEAGATAISLSAVLYENIINSKERERAEGLMNECEKSLRKSVQSLTIYSRTGLTSNNGVMGKNVRQLVSNISGRVPDMEMAAKRIKTNVKAWKTSQTHLKTLAKQAANAGRSSWSRFKNSKDIXKAFSGPTLAMTKGARMLSAVSAGVFLLVDAYGLVRDAKHLKKGGKAQLASEIRKKASLLEEELQNLSKLYNELKDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQM7</accession>
    <name>A0A6I9XQM7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neuroligin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106544576</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916357.1">
        <property type="nucleotide sequence ID" value="XM_014060882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQM7"/>
    <dbReference type="GeneID" id="106544576"/>
    <dbReference type="KEGG" id="tsr:106544576"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000460">
        <property type="entry name" value="Nlgn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01090">
        <property type="entry name" value="NEUROLIGIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013916357.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="32720" checksum="23F038FEA11B11AF" modified="2020-10-07" version="1">MAPGEGPVCAFLSAVGRDPNQPVPQDTKFIHTKPNRFEEVVWTKFNTKDKQYLHIGLKPRVRDNYRANKVAFWLELVPHLHNLHEPPYTSTTTRVPPYVTRWPSTRVGPSTTRQPIATLPPDEDDVARPPRYVPFPGDTRDYSTELSVTVAVGASLLFLNILAFAALYYKRDKRHELRSGRRLSPQRSAANDLVHGGQEEEIMSLQIKHLEHDAHDLEPLRPHEILRPACPPDYTLALRRAPDDVPLMTPNTITMIPSTITGMQALHPFNTFPTGHNNTLPHPHSTTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSP8</accession>
    <name>A0A6I9XSP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PSMB3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913708.1">
        <property type="nucleotide sequence ID" value="XM_014058233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSP8"/>
    <dbReference type="GeneID" id="106542487"/>
    <dbReference type="KEGG" id="tsr:106542487"/>
    <dbReference type="CTD" id="5691"/>
    <dbReference type="OrthoDB" id="204949at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000003">
        <property type="entry name" value="Proteasome subunit beta type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913708.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23018" checksum="AB763ACED7F0D205" modified="2020-10-07" version="1">MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVSTDFQKIFPMGDRLFIGLAGLATDVQTVAQRLRFRLNLYELKEGRQIKPQTLMSMVSNLLYERRFGPYYTEPVIAGLNPITYEPFICSLDLIGCPMITDDFVVSGTCTEQMYGMCESLWEPDMEPDHLFETIGQSMLNAVDRDAVSGMGVIVHVIEKDKITTRTLKARLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVL3</accession>
    <name>A0A6I9XVL3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="23">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">ATM</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 23">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 23">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="23"/>
    <dbReference type="RefSeq" id="XP_013917836.1">
        <property type="nucleotide sequence ID" value="XM_014062361.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106545720"/>
    <dbReference type="KEGG" id="tsr:106545720"/>
    <dbReference type="CTD" id="472"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007127">
        <property type="term" value="P:meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904358">
        <property type="term" value="P:positive regulation of telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000011">
        <property type="entry name" value="Serine-protein kinase ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000015">
        <property type="entry name" value="Serine-protein kinase ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 23" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 23 29" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18 23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 23" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 23" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="26">
        <location>
            <begin position="1932"/>
            <end position="2562"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="25">
        <location>
            <begin position="2682"/>
            <end position="2995"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="27">
        <location>
            <begin position="3020"/>
            <end position="3052"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="1608"/>
            <end position="1635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU365027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013917836.1"/>
        </source>
    </evidence>
    <sequence length="3052" mass="349660" checksum="9FE98DEE741CC091" modified="2020-10-07" version="1">MSLAIHELLTCCHQLGSDKAMERKKEIEKFKRLICDPETVQQLDRNSDSKLGKQMNWDAVFRLLQKYIQKEAESIKLSKPTTSASTQATREKKMKQISSLVKYFIMCANKRAPRIKCQELLDYVIETINESSRYAIYGADCNSILLKDVLKVRKYWCEISPQQWSDLQNLYFKLFLNPSGDVNKVLVARIIYTLTRGLCFQTNKFNSDILNVFSKAVHRARQERNLAGLEHMFAAINVFLPIYAMNYRMHVCKTGEEILSTVLFIWAQYKPKDALKKQIIQFVQFQICVHHPNGAKTQEEGAYSSTKWQHTLYNLYDLLANEITLISNRGKYSSGSHSIVLKDNLVELMADSCHQVFTEGTKVLEVTQSYGVTQQEDGSGEGPSKRRRIELGWEVIQEHLQKSQNNFDVIPWLQITTRLVSKYPRSLPDNELTNLLNILYQLLLQQRRGERTPYVLRCLKEVALCQSQKTDLSITQKFELQRTWSRIWSLVDRSLNLRQSEMESFELLGVLFQRNLITMDKEIWRIFAGSVCKPSSFAVQCLASAMTALEIPETLKTGPFQNDWEGNATCTLKERIVKWLLLVEEVMEDSMELPPVLCSDFPNLELQKILVSLTMKSCRAAMAFFNNAQECMEHNQEKEETSLSEIEAFYLQTTFNEANIFTNMTNVSENKRSSCLIVSQKLEETLKHNLMMMSDQLLCNVSAETIPVEMRMRWTSILTGVLGCYCYAGILTEEEACKLDLFQKAKLCFNYVEESISQSKNKLNEDSRIVSLRNLILQCTSCLCNCTQKNPKKSLSTIFLHLLTSKLMDDLTSICKLMMFSTGKSSETGEIDLMDDFPDESRMEEGTQIVFDLFDDECTEVGEVNEVGDVHTLTGATSPLSEEHLSKQDLLLLDILKFLCIASTATTKQTVPFRCSEIWRKLLVLIDGDSFDFIKPLHLHVYLVLLKELPRGENPLPEDYVVSLVKLLPDICSFYRRDQDVCVAILHNLLPMVNILGTFSRNSEDLKEARCQVLTVVGAFWHLANEGKCSVPLRIALVNCMTAFLHADPGLKWAVLTVKGENLPVRDAFPQFLADNNFQVCTIAAKSAISLFQDVKLRETTGLVKGLPLTFQQRAFENLYIKVQEGAKTLMKAHGTGNQESHPDDEHNRKTVLLMLITMILYSSPVCEKQALFAIFQCIKENGLDPQLVRKVLENISGSFGYKNTEDLMSTHLDYLVFEWLNLDYSLSDFPYVLLNCSTLEEFYRSCYKILIPQLIIRDQFKEIKSIASHIEMEERPLLAQCFPKILVNILPYFAYQNHEDIEFTQKSNTASRIYDTLRDENYLGKQLTDSLFQSSLPEIVVEVLMTLHEPINIKSEENSDLFKFTGDLDPAPNPPYFPSFIIQATLDYLNRCFKMKSNGIIAILSKNPESFHKILFAVWKQVSGTKNNYKKHRMLMIYHFFVNLLLKEIKGGLGGAWAFVLRDVIYSMIHHISNRPNPLTGVSLRSFLLCCDLLNHVCHTAVQYCGDALASHLHVVVGTLIPVVGEQPETEEKVLGLLKYLVIDNQNHEYLYQAIKLLDPFPDHPHFKELRATQQKIKYSTGKFSLLQEINHFLSINISDSLFLTRLEGLNELHKQLEVRKEQIMELMKKFQENPRDCIIVKLMAYLLQLSKTTVHSTDAILEAVGSCLGEIGPINFSTIALQPSKNGPSSNTCDLFEDRELQWVFVILTLINNSLIDQSIEVRSAAAVCLRNILATKIGHTFSEMYKTQTDTMLKYLHPFRTSKKKLLEVPGKVRETPPEDLDRTTLWIPCSESHEIWIKTLTCALLDSGMITSEILLLLKPLCQVHADFCQTVLPYLIHDILLHDSNESGHNLLSAQLQTFFMTCCKHSSSSRSSTPANSDSDSETLVHLDKISRRTMLAVVDYLRHQKKNPSSTIFDDSFWLELSYLQVAMVAQSCSAHFTALLYAEIYADKIGRQQERSVSKAAKKLTFEEGSQDSTLTCLSEKSKEETGISLQDLLMDIYRSIGEPDSLYGCGGGKMLQPLARIRTYEHEGMWGKALVTYDLQTSLPPSIRQAGLVQALQNYGLCNTLSIYLKGLEQQNEESSIELQEIRYQAAWRNMQWDQISSVKDEIEQRGYHESLYDALQCLRDRDFSTFYGRLKCARIKEVEELLKGSLESVYSLLPTLCRLQTIGELEYVGQLFSGSETNNQLHNLHLKWQKQSQLLQDSDFAFQEPIMALRTVIFKLLLEKENENDQRDCIKNILTEHLVELSKLARMANNSQLPERAIYEVKQYSLTRHGVSEWKLEEAQVFWAKKEESLALSILKDMIKKLDENWIEVEKDPHLKLIYTECLRLCGSWLAETFLENPTIIMQNYLEKAVKIAGNHNDDSSDELKRGKMKAFLSLARFSDTEYQRIEDRMKSSEFENKQALLKKAKNEVVLLREHKVQTNRYTVKVQRELELDECAIRALEEDRKRFLRKAVENYIMCLLSGEEHDMWIFRLCSLWLENAGLSEVNAMIQKEAQKIPSYKFLPLMYQLAARMGTRMNKFHEILNNLIARISLDHPHHTLFIILALANANKDELLTKPEVTRSGGLIKNVPKETSPLDMDRMKAASSIINIVKRKRPDMVLKVEALCDAYITLANMDATPWKTHRRAINIPSDQPLIKLKNLEDVAVPTMEIKVDPSGKYENLITIRSFKAEFRLAGGVNLPKIIDCVGSDGQQRRQLVKGRDDLRQDAVMQQVFQMCNTLLQQDADTRKRKLTIRRYKVVPLSQRSGVLEWCTGTIPLGEFLINTDSSAHKRYRPQDYSSLECQKKMLDAQKKDFEEKYTLFMDICQHFQPVFRYFCMERFLDPAVWFEKRLAYTRSVATSSIVGYILGLGDRHVQNILLDEESAELVHIDLGVAFEQGKILPTPETVPFRLTRDIVDGMGIAGVEGVFRRCCEKTMNVMRNSQEALLTIVEVLLYDPLFDWTMNPLKALSLQQRSEDEADVSSNFSSANQGCSKKANNENQLFNKVAERVLIRLQEKLKGMEEGTVLSVAGQVNFLIQQAMDPKNLSRLFPGWKSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWB8</accession>
    <name>A0A6I9XWB8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Dermatan-sulfate epimerase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106546179</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dermatan-sulfate isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918447.1">
        <property type="nucleotide sequence ID" value="XM_014062972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWB8"/>
    <dbReference type="GeneID" id="106546179"/>
    <dbReference type="KEGG" id="tsr:106546179"/>
    <dbReference type="OrthoDB" id="5946629at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047757">
        <property type="term" value="F:chondroitin-glucuronate 5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.100">
        <property type="entry name" value="Chondroitin AC/alginate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008929">
        <property type="entry name" value="Chondroitin_lyas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052447">
        <property type="entry name" value="Dermatan-Sulfate_Isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15532">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15532:SF3">
        <property type="entry name" value="DERMATAN-SULFATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48230">
        <property type="entry name" value="Chondroitin AC/alginate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026760509" evidence="9">
        <location>
            <begin position="25"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013918447.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25876" checksum="1517423B0594B676" modified="2020-10-07" version="1">MRTHTRGAPSVFFIHVVCFALICSTDNNPSTVVPFVNANYDNYPMLYFSRREIQDLRYKAGTTHQHIAARLVEAVQIMLSNSLEYLPPWDPKDFSARWNEIYGNNLGALAMFCILYPDNVEAIDMAKDYMERMAAQPSWLVKDAPWDEVPLAHSLVGFATAYDFLYNYLSKSQQEKFLEVIANASGYMYEASYRRGWGFQYLHNHQPTNCLALLVGSLVLMNQECG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWK2</accession>
    <name>A0A6I9XWK2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Alpha-2,8-sialyltransferase 8B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ST8SIA2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915018.1">
        <property type="nucleotide sequence ID" value="XM_014059543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWK2"/>
    <dbReference type="GeneID" id="106543507"/>
    <dbReference type="KEGG" id="tsr:106543507"/>
    <dbReference type="CTD" id="8128"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000001">
        <property type="entry name" value="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF30">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="74"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013915018.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="33217" checksum="6146454F8C8A0B1E" modified="2020-10-07" version="1">MALSVFLSSSSSKRKHILKFLDAEKDISVLKGTLKPGDIIHYVFDRDSTMNVSQNLYALLPRTSPLKGKHYRTCAIVGNSGILLNSGCGKEIDAHSFVIRCNLAPVQEYIHDVGMKTDLVTMNPSVIQRAFEDLVNDTWREKLLQRLHSLNGSILWIPAFMAKGGKERVEWVNELILKHHINVRTAYPSLRLLHAVRGYWLTNKVYIKRPTTGLLMYTLATRFCNQIYLYGFWPFPQGPDQSPVKYHYYDSLKYGYMSQTSPHTMPLEFKALKLLHLQGALKLTVGPCNGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY01</accession>
    <name>A0A6I9XY01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FERM domain-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FRMD8</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921109.1">
        <property type="nucleotide sequence ID" value="XM_014065634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY01"/>
    <dbReference type="GeneID" id="106548291"/>
    <dbReference type="KEGG" id="tsr:106548291"/>
    <dbReference type="CTD" id="83786"/>
    <dbReference type="OrthoDB" id="2142533at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051594">
        <property type="entry name" value="KRIT1/FRMD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057096">
        <property type="entry name" value="KRIT1_FRMD8_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13283:SF10">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13283">
        <property type="entry name" value="KREV INTERACTION TRAPPED 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24522">
        <property type="entry name" value="KRIT1_FRMD8_FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="2">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921109.1"/>
        </source>
    </evidence>
    <sequence length="420" mass="48290" checksum="085C52424C9C88B6" modified="2020-10-07" version="1">MDNLPSVTAHELQRMVLDVLKLPESAQEIFSLWLVSPFLGVQLKPKHQPYKICRQWQELLLRFTSCPVDEIVEDEPSLQFRRNIFFLRRKELQIQDEDVLRLLYEEAKHNVLEGRFPCDPADCDALGALVCRINLGPYHPEQHTASFLKEKLDCFLPSHLCHRGHKLFSSLRKRTGSRPPVSEQGLLRAFREVPEAGACEEHATLLRLYHAYLQKCHELPYYGCAFFFGEIDKPVQGLLHRAGRKAVTVAISLEGVYVMDTKEKYVLLGLRFQQLSWDHTVPNEEEPILWLEFDGENEGQQVNKLLKVYSKQVCVCWGSYLNFNGCVCVSHSNLHYNYRIFQSPATASVPTSDQQPPKIQRQSSVLCSRIQRLATIDYVEEGKEIKRVKPRRTTSFFGRQLSNAPTSYSAVRAAENLEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY76</accession>
    <name>A0A6I9XY76_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FK506-binding protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FKBPL</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919147.1">
        <property type="nucleotide sequence ID" value="XM_014063672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY76"/>
    <dbReference type="GeneID" id="106546725"/>
    <dbReference type="KEGG" id="tsr:106546725"/>
    <dbReference type="CTD" id="63943"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF10">
        <property type="entry name" value="FK506-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="255"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919147.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="34526" checksum="ED01BF30FFDBFEDB" modified="2020-10-07" version="1">MEPKGMASSEKACLKSQTAGEREAGKKPDQRTPICDKNIQDAGKKDLGDKVKENPPLPIQSQPGVCWPCPDGTFIKLILAAGTGLDKPKEGSICQVFIEASHEGPLSYPSSRWAEVELGGGDAEWDGVVDRGLETMLAGERAELRLSEGSTIAVQLASFTEAKDSWEMSASEKWDLVLSNKDRGSELYRAGAIAAAARRYARALHLLVVAAPPPDYDQIKAELHTNLAACQLRLHQPANAAQNCSKTLALQPANPKALFRRGLAYDAMNDLEGAAQDLKGVLQVEPGNRAARRELERVTERIRARDARLAQAMQKMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYK2</accession>
    <name>A0A6I9XYK2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 51</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106546836</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919282.1">
        <property type="nucleotide sequence ID" value="XM_014063807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYK2"/>
    <dbReference type="GeneID" id="106546836"/>
    <dbReference type="KEGG" id="tsr:106546836"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013919282.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="24136" checksum="893D4C1AF666678F" modified="2020-10-07" version="1">MRISGRGPLCFASTAGKACVHWNYTKASLQAPPLDFSFRGISFMQDLLTEEPRPGLRAIRRLEGGRLLTQAIRLNNNTLNDLKDFPDIMEKLLEYPLDIYWIDLSFNDLPSIDPVLTTYYNLRNLNLHGNSIQSLSEVDKLAVLPHLRSLTLHGNPIEEEKGYRSYVLSTLPQLKSFDFSGVTKLDRSTAVVWRRMNIKPKVVRKKRDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZE8</accession>
    <name>A0A6I9XZE8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Complement factor B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106544271</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915983.1">
        <property type="nucleotide sequence ID" value="XM_014060508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZE8"/>
    <dbReference type="GeneID" id="106544271"/>
    <dbReference type="KEGG" id="tsr:106544271"/>
    <dbReference type="OrthoDB" id="9981647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006956">
        <property type="term" value="P:complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46393:SF1">
        <property type="entry name" value="COMPLEMENT FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46393">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="VWFA" evidence="7">
        <location>
            <begin position="34"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="188"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013915983.1"/>
        </source>
    </evidence>
    <sequence length="421" mass="46936" checksum="938C80C24A82741C" modified="2020-10-07" version="1">MFPLLGLLGLIKPHQLMPCSTLHFDFPAHELKPGTNIGKGLRSVYEMMIAQEEEEVRRGLNPPPVSTTTRHIIILLTDGNYNLGGSPSLIIQQIKKFLKIGSSSPNPRDDHLGKMTIHEPDGFISPLLHLSRLDVYAFGNGPDVDVENINELASHKPNERHGFILKDLSELQEVLEESLGEEPRQEICKGVIVSDQYILTAAHCLAGIKDISVILGTTNFAVAEIQYHPEYNSEKIKNKDSPEFYNYDVALVKLKEKHLPSFTSSSNGSCCATTQTYFQTSLPQPASFKICCHYNYQSFPATMVAQKMACNLDAAKAKGYVNVANISEMVNEHFLCTGGIDPQVDPNVCSYDSGGPLLIQRRLRYVQVSGNLSQIQPNRPTFQPSNITGSLFPTHVRDFHINIFKILPWLKEQLGTEVEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZV1</accession>
    <name>A0A6I9XZV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106544419</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916153.1">
        <property type="nucleotide sequence ID" value="XM_014060678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZV1"/>
    <dbReference type="GeneID" id="106544419"/>
    <dbReference type="KEGG" id="tsr:106544419"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000024">
        <property type="entry name" value="Vomeronasal 2, receptor 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="571"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="641"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="687"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="730"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="765"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="791"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="571"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013916153.1"/>
        </source>
    </evidence>
    <sequence length="835" mass="95450" checksum="F4E5278CCE98D70E" modified="2020-10-07" version="1">MYPILCNRIDALQIPHEYYQPALKGYYKAVQVSRADTLSVSVVIKNYQQVLAFLYAMKEVNENPRLLANVTMGFHIYDSYFNDRLTFQTTMNLLYSPKKTVPNYKCNSGKNLIAITGALHSETSLHMATISSIYKIPQVSYYLFDPLFSTKTQFPFIYRVVPNEKHQYMGIVKLLKHFQWIWVGIITLDDDKGESFVRTLVPMLSQNGICSAFTDRMPSITNFLEIHDSYMETEKTLNFFRINEVKALLVYAETHSVLSLKFLLTQNNDIADTLDRVWIMTAQWDFPALQFHKDWDTMVFQGTLSFAAHSREVLGFQHFLTTIKPNLLKKDNFINDFWEQAFNCLFSNYYTGEEHTEICTGREKLQNLPGPFFEMKMTAQSYSVYNAVCVVAHALNAMRSLDSQQRMMRTLGSPQPWQLHSFLRRISFNNSAGDLVSFDEQCELEGGFDIINWITFSNKSFSRVKVGKLNPHVLSTKQLYINDGKILWHSNFNKTPPFSICNDHCHPGSVRKKKDGKPFCCYDCVSCPKGKISNQNDMDNCIQCPDDQYANINQDGCLPKSLNFLFYDEPLSIGLAFMAALLSAITVLVLGIFVKYQHTPIVKANNRYLTYTLLISLLLCFLCSFLFIGKPHTIICIFRQTTFGIIFSVAISSVLAKTITVVLAFVATKPGSRIRSWVGKRLATFMILFCSVFQLLLCIIWLCTAPPFLDLDMHSLTKEIVVECNEGSDFMFYCVLGCLGILALISFIVAFFARTLPDTFNETKFITFSMLVFCSVWITFVPTYLSTKGKYMVVVEIFSILTSSAGLLGCIFFPKCYIIALKPELNSKELIIRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZW9</accession>
    <name>A0A6I9XZW9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Papilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106547384</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the papilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920002.1">
        <property type="nucleotide sequence ID" value="XM_014064527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZW9"/>
    <dbReference type="GeneID" id="106547384"/>
    <dbReference type="KEGG" id="tsr:106547384"/>
    <dbReference type="OrthoDB" id="9948486at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22635">
        <property type="entry name" value="Kunitz_papilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000017">
        <property type="entry name" value="papilin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027025014" description="Papilin" evidence="13">
        <location>
            <begin position="20"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="773"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="985"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="1108"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="592"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="838"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="606"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="842"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="878"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="39"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="43"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013920002.1"/>
        </source>
    </evidence>
    <sequence length="1159" mass="129425" checksum="8E6B07451EB1B8E0" modified="2020-10-07" version="1">MVLLILLVALAAMSPSTLAARKAKRQNDFWGSWGEWGECSRSCGGGISLRQRHCYSRRTDGGSSCIGPAQSYRSCNIQVCPEGSRDFRAEQCAEFDGTEFQGKKYKWLPYYGAPNKCELNCIPKGENFYYRQKEAVIDGTTCEPGMNDVCVQGTCHAVGCDKMLDSSQKEDKCLQCGGNGRACFEVKGLFDVPNLPKGYNQIFVIPMGATSIRIKEATPTRNFLAIKNVQGTYYLNGQWTIEYSRALPIASTVVHYDRGSEGDLAPELLYARGPISEPLIIELISQERNQGVHYEYYLPFQRQTSIYSWGYGSWSDCSAECGGGYQSRLVFCTIDNEAYPDYMCRDKPWPGNNRTCGLQPCPQTKRVSYLYLPGAWSRNRAWNLQTKSWKTGDWSQCSATCGGGFQSRSVYCASYNGHHFQDAVDDTECASLTEKPRSAQPCNLQRCATWVAGPWSECSVSCGEGIQTRTVRCQVEQGPQILDIACLREPKPQHTQPCIGENCIQEIGWHIGDWGLCSKSCSSGIRTRQVICADGDSKFYGHEICQEIQPQIPTMLGSCNTQPCHLPQQVPSMQDITGFNISRRTLLTRYHPDQGTEVSRHERISQAGSTHRQVQLNESPRSNFLPLGWDSYSDSSVSRRSHSYPNGGSNDQDCNHTPHGCCSDGRTTAAGPFGQGCPLDSCHLSRYGCCPDGVSHSQGPNQEGCTQYYSDVDISRNPVTPTPTTSQVLAQQLPTEECRGSMYGCCYDNIASALGPQGEGCPTRFSQSYPVQCMLPSAHGSCTDWTIRWYFVSDVGKCNRFWYGSCHGNKNNFATEEECMNTCHGSMGRSHVSLHHHVASPNIPSSSQLQEGKTEVNPQQFGRTITHRTGNEGHSDRTSSNQDALPESEQGTSLLENQSENSHRSRTLDEIKRDTVDHLHGLSETVISRHSEKQLTVNGQTTQHEADEWRRESGADALTRRRFGYQTPPESTFQYSLNRIFLDGPESSAVEAGLGQSIRLLCKTDNSHFSRVEWQKDGQPISSDRHTYQSDNSLVVSHLRTEDAGIYTCTISNGRVERRQIELLIKEIPQSTFTQGRRHQLTHDQNHQHRVFGTGTNDRWSHVSSLHPQITYRLKMYKNEPSVVDASIGEQVRLPCRVEASPSLTIEWQKDSRPISSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y162</accession>
    <name>A0A6I9Y162_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106540033</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910485.1">
        <property type="nucleotide sequence ID" value="XM_014055010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y162"/>
    <dbReference type="GeneID" id="106540033"/>
    <dbReference type="KEGG" id="tsr:106540033"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF99">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="113"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910485.1"/>
        </source>
    </evidence>
    <sequence length="630" mass="71758" checksum="EA70791703517A77" modified="2020-10-07" version="1">MSGLQKTFSILKNSVRAFPVLFTDHIPVLLNMYCSSIYVIQRTVEDIVNLEQPKGKHASRDIHAWTASFQNTTVLESLGETNWETNWTNESKDAAGFFDYQHVQVPFEITLWILLASLAKIGFHLFEKLPSVVPESCLLIVVGLIMGGIIFGLNDKSPPVMDSDIFFFYLLPPIVLDAGYFMPSRPFFGNIGTILLYAVVGTIWNVFGIGFSLYLICQADAFQLGDISLLHNLLFSSLIAAVDPVAVLSVFEEIHVNQKLHILVFGESLLNDAVSVVLYRLFRSFCEMPTIRTVDVFVGIGKFFVVGIGGLLVGLLYAVTAAFTTRFTKNILVIEPLFVFLYSYLSYLTAEMFHLSGIVAIISCALGMKRYVEANISLKSHTTIKYFMKMWSSVSDTLIFIFLGVSTVGDNHEWNWPYIFFTLIFCLLWRALGGAQFKFKICIQICSLLLHHLYLSSLVIYLMIEPTFELDLPIPPSVFSLMLQGMTIRPLVDWLHVTRKQESAPTVGEQIHIRFLDHLLAGIEDISGHWGQYYWKDKLEYFNSKHLQKYLLREYKQPKSSIVLLHEKLERKRAIELAEAGQLSWRPSQKALLKKDNPVTISVDTESLSQDEMNEIQKILEKNLYKTRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y204</accession>
    <name>A0A6I9Y204_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106547794</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920526.1">
        <property type="nucleotide sequence ID" value="XM_014065051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y204"/>
    <dbReference type="GeneID" id="106547794"/>
    <dbReference type="KEGG" id="tsr:106547794"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="35"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013920526.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="35551" checksum="5D8454FA8F3CEB43" modified="2020-10-07" version="1">MNITEFIILGFGNFGQWQSIIFFIFLVFYIVAMAGNILIVILVAIDQRLHTPMYFFLGNLSCLETCYISNILPKMMVGFLTDENTISFNGCFLQFFCFGLLVATECYLLSVMSYDRYLAICKPLHYATNMNARICIQLAAFSWINGLIAALILLSLMLQLTFCGQNEINHYFCDTLPLIKLSCSDTNLVEFTSFSFTFVFTLPPFILTVTSYIYIIITILRIPSTIGRKKAFSTCSSHLIVVSMFYGAIMIVYLLPKSEALSEVSKVFSLLYTVLPPVANPLIYSLRNKEVKDAMKKISKKFIPFRFYRGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y232</accession>
    <name>A0A6I9Y232_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Transcription factor Sp5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106548250</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921067.1">
        <property type="nucleotide sequence ID" value="XM_014065592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y232"/>
    <dbReference type="GeneID" id="106548250"/>
    <dbReference type="KEGG" id="tsr:106548250"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22541">
        <property type="entry name" value="SP5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000061">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF177">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="169"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="199"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921067.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="30367" checksum="18B38E0EB419680B" modified="2020-10-07" version="1">MFQLWSNEVPSRSHTTMAFGRPKMPYHEQMVASSCGASAHELPLTPPAEAIYSLEWSPVKMLAPTVHPSYTFPEAQDSSSFLQGPWSLGPAVPPAPSSTDDAPWWTLQQPSNFSLGHPLVLSPQPPLAPFLQSPPKGLLSTARRCRRCKCPNCEGASRSHEEPGRKKQHVCHLPGCGKVYGKTSHLKAHLRWHAGERPFICSWLYCGKSFTRSDELQRHLRTHTGEKRFGCQLCPKRFMRSDHLAKHVKTHQGKRVRNVAVVTAAAVGIKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2C2</accession>
    <name>A0A6I9Y2C2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Sulfotransferase</fullName>
            <ecNumber evidence="19">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC106547921</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>alpha-D-glucosaminyl-[heparan sulfate](n) + 3'-phosphoadenylyl sulfate = N-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:21980"/>
            <dbReference type="Rhea" id="RHEA-COMP:9830"/>
            <dbReference type="Rhea" id="RHEA-COMP:14602"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:58388"/>
            <dbReference type="ChEBI" id="CHEBI:140572"/>
            <dbReference type="EC" id="2.8.2.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:21981"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; heparin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Golgi apparatus</location>
            <location evidence="14">trans-Golgi network membrane</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sulfotransferase 1 family. NDST subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013920671.1">
        <property type="nucleotide sequence ID" value="XM_014065196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2C2"/>
    <dbReference type="GeneID" id="106547921"/>
    <dbReference type="KEGG" id="tsr:106547921"/>
    <dbReference type="OrthoDB" id="8958249at2759"/>
    <dbReference type="UniPathway" id="UPA00756"/>
    <dbReference type="UniPathway" id="UPA00862"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019213">
        <property type="term" value="F:deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015016">
        <property type="term" value="F:heparan sulfate N-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030210">
        <property type="term" value="P:heparin proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000176">
        <property type="entry name" value="bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021930">
        <property type="entry name" value="Heparan_SO4_deacetylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF30">
        <property type="entry name" value="BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12062">
        <property type="entry name" value="HSNSD-CE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Heparan sulphate-N-deacetylase deacetylase" evidence="21">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="20">
        <location>
            <begin position="276"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="16">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="504"/>
            <end position="508"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF12062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013920671.1"/>
        </source>
    </evidence>
    <sequence length="553" mass="64624" checksum="EE09D206322A8C51" modified="2020-10-07" version="1">MKVDDVKALFDTQNELRTHIPNFTFNLGYSGKFFHTGTDAEDEGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLADQMAMNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWGIKVTSTEEYPHLKPARYRRGFIHSGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFRHLVRFLNSWTNLKLQTLPPVQLAQKYFQIFSEEKDPLWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLGMHPDLSSNYPSSETFEEIQFFNGHNYHKGIDWYMEFFPIPSNTTSDFYFEKSANYFDSEVTPRRAAALLSKAKIITILINPADRAYSWYQHQRAHDDPVALKYTFHEVITAGPETSQKLRTLQNRCLVPGWYATHIERWLNNFHTNQILVLDGKRLRTEPAKVMETVQKFLGVTNTIDYHKTLAFDLKKGFWCQLLEGGKTKCLGKSKGRKYPEMDLDSRAFLRDYYRDHNIELSKLLYKIGQTLPTWLREELQNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2N5</accession>
    <name>A0A6I9Y2N5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tyrosine-protein kinase Srms</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SRMS</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920836.1">
        <property type="nucleotide sequence ID" value="XM_014065361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2N5"/>
    <dbReference type="GeneID" id="106548056"/>
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    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="51"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="120"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="230"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013920836.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29992" checksum="5E08E6FD925B06AB" modified="2020-10-07" version="1">MDNFIRKHLHFFHTFWNKCVPNGASSNRSLESWEPIPGSLHPTLDPDPAEDKASLRGVLYNYTAKNAEELSVTRGDKLYVLKEEGGYVLARRFSGDRVVGYVPASYISKGSEEPASHQSWYINGITRNKANQLLLSPPNHHGSFLIRDSESNKGEYSLSVRNHGKVRHFRIFSNPAGSFYLEKAHVFPSLEELLAYYRANWKIIDCPLSQPCIQKRTSEVDQWERPRSEFQLWRKMGEGCFGEVWEGMWKNTVPVAIKIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4S6</accession>
    <name>A0A6I9Y4S6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GTPBP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912530.1">
        <property type="nucleotide sequence ID" value="XM_014057055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4S6"/>
    <dbReference type="GeneID" id="106541569"/>
    <dbReference type="KEGG" id="tsr:106541569"/>
    <dbReference type="CTD" id="54676"/>
    <dbReference type="OrthoDB" id="248233at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04165">
        <property type="entry name" value="GTPBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03694">
        <property type="entry name" value="GTPBP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03708">
        <property type="entry name" value="GTPBP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000058">
        <property type="entry name" value="GTP binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000014">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000091">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035531">
        <property type="entry name" value="GTPBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF3">
        <property type="entry name" value="GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="165"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="17"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013912530.1"/>
        </source>
    </evidence>
    <sequence length="604" mass="66175" checksum="87027F70DD6AEB56" modified="2020-10-07" version="1">MDSRVSELFGGCCRPVGNGGALRGRGGGSGGGSSKGKKKNGRYRGGKANNPPYLPPEAEDGNIEYKLKLVNPSQYRFEHLVTQMKWRLQEGRGEAVYQIGVEDNGLLVGLSEEEMHASLKTLRRMAEKVGADITVLREREVDYDSDIPRKITEVLVRKVPDNQQFLDLRVAVLGNVDSGKSTLLGVLTQGELDNGRGRARLNLFRHLHEIQSGRTSSISFEILGFNSKGEVVNYSDSRTAEEICESSSKMITFIDLAGHHKYLKTTIFGLTSYCPDFAMLVVSANTGIAGTTREHLGLAMALKVPFFIVISKVDLCSKATVERTVKQLERILKQPGCNKLPLLVTSDDDAVTAAQQFAQSPNITPIFTLSSVSGENLDLLRVFLNILPPLTNSKEQEELMQQLTEFQVDEIYTVPEVGTVVGGTLSSGICKEGENLVVGPTDDGKFLQLKVCSIQRNRSACRVLRAGQAATLALGSFDRSLLRKGMVMVSPEMNPTICSVFEAEIVLLFHATTFRKGFQVTAHVGNVRQTAIVENIHGKDKLRTGEKAVVHFRFIKHPEYLKIGAKLLFREGVTKGIGHVTSIQAITTGENGLEHNLCPDPVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5G7</accession>
    <name>A0A6I9Y5G7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Plexin-A1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106547232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919798.1">
        <property type="nucleotide sequence ID" value="XM_014064323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5G7"/>
    <dbReference type="GeneID" id="106547232"/>
    <dbReference type="KEGG" id="tsr:106547232"/>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12790">
        <property type="entry name" value="RasGAP_plexin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000005">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF35">
        <property type="entry name" value="PLEXIN-A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plexin cytoplasmic RasGAP" evidence="1">
        <location>
            <begin position="1"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Plexin cytoplasmic RhoGTPase-binding" evidence="2">
        <location>
            <begin position="160"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919798.1"/>
        </source>
    </evidence>
    <sequence length="584" mass="66904" checksum="8F14654CC47B99F3" modified="2020-10-07" version="1">MRVLFPGIEDHPVLKEMEVQANVEKSLTLFGQLLNKKHFLLTFIRTLEAQRSFSMRDRGNVASLIMTALQGEMEYATGVLKQLLSDLIEKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLYKFLKECAGEPLFMLYCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIDYKMLTLNCVNPENENAPEIPVKVLNCDTITQVKEKLLDAVYKGVPYSQRPKAGDMDLVSTIPSDPMPFSSIGAEWRQGRMARIILQDEDVTTKIDNDWKRLNTLAHYQVTDGSSVALVPKQNSAYNISNSSTFTKSLSRYESMLRTASSPDSLRSRTPMITPDLESGTKLWHLVKNHDHMDQREGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETIFSTAHRGSALPLAIKYMFDFLDEQADKHQINDYDVRHTWKSNCLPLRFWVNVIKNPQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHKLGKDSPSNKLLYAKDIPNYKSWVERYYADIAKMPAISDQDMSAYLAEQSRLHLSQFNSMSALHEIYSYITKYKDEILAALEKDEQARRQRLRSKLEQAIDTMALSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6P3</accession>
    <name>A0A6I9Y6P3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial tRNA-specific 2-thiouridylase 1</fullName>
            <ecNumber evidence="4">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TRMU</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013914170.1">
        <property type="nucleotide sequence ID" value="XM_014058695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6P3"/>
    <dbReference type="GeneID" id="106542853"/>
    <dbReference type="KEGG" id="tsr:106542853"/>
    <dbReference type="CTD" id="55687"/>
    <dbReference type="OrthoDB" id="3685at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061708">
        <property type="term" value="F:tRNA-5-taurinomethyluridine 2-sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000057">
        <property type="entry name" value="Mitochondrial tRNA-specific 2-thiouridylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000104">
        <property type="entry name" value="Mitochondrial tRNA-specific 2-thiouridylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.280:FF:000001">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00144">
        <property type="entry name" value="tRNA_thiouridyl_MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001138">
        <property type="entry name" value="PRK00143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like central" evidence="16">
        <location>
            <begin position="233"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like C-terminal" evidence="15">
        <location>
            <begin position="305"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013914170.1"/>
        </source>
    </evidence>
    <sequence length="419" mass="47572" checksum="61D87D02A2E69AD3" modified="2020-10-07" version="1">MIGAAGRHVVCAMSGGVDSAVAALLLRRRGYDVTGVFMNNWDLVDEKGICQGNQDCEDAYKVCQILNIPFRQVSYVKEYWNEVFSYLLNEYEVGRTPNPDIICNKNIKFNYFLHYAMNNIGADAIATGHYARTSLEDNEVFQQKYQQKPRGLFRNRFEIRNAVKLLQGADHNKDQTFFLSQIPQEALQKTIFPLGDLTKDFVKKIAAEHQLYHVLKKKESMGLCFVGKRDFSEFLLEYLQPRPGNFVSIEDGKVLGTHKGCFLYTFGQRAKLRNCSNAWFVVDKNISSGDVFVAPSRDHPALYRDLLRTSRVHWIAEEPPAELIQNKMMECNFRFLHLMALVPCTLTLNQDGSVWLTLAKPIRDLTAGQFAVFYKGDECLGSGMILRLGPSVFTLQQGKNRETAGVNLPSDEVTVHPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y793</accession>
    <name>A0A6I9Y793_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(4) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">KDM5B</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 2-oxoglutarate + 3 O2 = L-lysyl(4)-[histone H3] + 3 formaldehyde + 3 succinate + 3 CO2</text>
            <dbReference type="Rhea" id="RHEA:60208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.67" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013914775.1">
        <property type="nucleotide sequence ID" value="XM_014059300.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543320"/>
    <dbReference type="KEGG" id="tsr:106543320"/>
    <dbReference type="CTD" id="10765"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16874">
        <property type="entry name" value="ARID_KDM5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15603">
        <property type="entry name" value="PHD1_KDM5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15687">
        <property type="entry name" value="PHD3_KDM5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000023">
        <property type="entry name" value="Lysine (K)-specific demethylase 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000035">
        <property type="entry name" value="PHD transcription factor Rum1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048615">
        <property type="entry name" value="KDM5_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047981">
        <property type="entry name" value="KDM5B_ARID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047978">
        <property type="entry name" value="KDM5B_PHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047979">
        <property type="entry name" value="KDM5B_PHD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF3">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21323">
        <property type="entry name" value="KDM5_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="22">
        <location>
            <begin position="10"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="21">
        <location>
            <begin position="75"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="307"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="23">
        <location>
            <begin position="451"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="1174"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="1469"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="198"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013914775.1"/>
        </source>
    </evidence>
    <sequence length="1529" mass="175068" checksum="58853DACE303B149" modified="2020-10-07" version="1">MAEFLPPPECPVFEPTWEEFADPFGFIHKIRPIAERTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWELQGCALKIPHVERKILDLFQLNRLVAEEGGFDLVCKDRKWTKIATKMGFAPGKAVGSHIRAHYERILYPYNLFQTGASLLAPDICSKLMCLVDDAVLEKCFQKPDITNGTKEKEYKPHDIPQRQSVQPSETCAPARRAKRMRSEATTNIKTEIEETTETKTHNLRRRMGSTPIKPDNDKEPQAIPKREVVEKKQWLWEADREKSKGRSKKNFNSVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPQCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLRPEEAEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGYAAEQLEDVMKKLAPELFESQPDLLHQLVTIMNPNTLMSHGVPIYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLNRYCVFSHDEMICRMAAKAETLDVVVASTVEKDMAIMIEDEKTLREAVHKLGVTESERINLEVLPDDERQCIKCKTTCYMSAVYCNCNPDLLACLYHVEELCTCPIYQYKMGYRHTVDELYPMMNALKLRAESYNEWASNVSEALEAKVNNKKSLMNFKALIEESEIRKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRLFVRQLHALPCVLNQTTLLKDLLNRVEDFQKYSEKLLSEEIPSASKLQELLEVSFDFDVDLPQLGELRLRLEQARWLEDVQNVCLDHTSLTLDDMRRLIDSGVGLAPHPAVEKAMARLQELLTVSEHWDDKARTLIKARPRQSLSSLEAAVKEIDVIPAYLPNGIALKDAVKKAKDWLQEVESLQAGGRVPVLDMLVELITRSRSIPVHLDYLPRLESLVAEVQVWKECASNTFLSEDSSYSLLEVLCPRCDIGTSGFKRKTKKTKESVPNGKKKSPKLESLCDLERALSECKETAAAMAMLGEARLKEMEALHSLRAINEEKIFSDEQNVEIKVCLCQREPAAPMIQCELCRGMFHTSCVSVSSNLQGPWVWLCPHCHRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQMLSSGNIKLLANKVDSGILYSKWQTMAGQLQETNKVSQTIGATSFSLPHEWNNRSIHLHSLFSTGQNCIPLHTISTELNELMMEAQLLQVSLPEIQELYEILFTKEKSVGPTSEKNDCQGKRDGICSMEKKLKRRFEREKFCSEMRARVRKIAPNKKKMKLTHLRELSKLEREGLFDIQHTGESQLLFSEMSFSEQEDSEDENAICPADNCLQPEGDEVDWVQCDGCCNQWFHQVCVGVSPEMAEKEDYICTSCTRKDSPYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7G4</accession>
    <name>A0A6I9Y7G4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 13-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106543600</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915130.1">
        <property type="nucleotide sequence ID" value="XM_014059655.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543600"/>
    <dbReference type="KEGG" id="tsr:106543600"/>
    <dbReference type="OrthoDB" id="103819at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051139">
        <property type="entry name" value="Mediator_complx_sub13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041285">
        <property type="entry name" value="MID_MedPIWI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18296">
        <property type="entry name" value="MID_MedPIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MID" evidence="8">
        <location>
            <begin position="979"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="129"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="572"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1087"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1143"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="320"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="603"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="615"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="651"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1087"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1143"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1168"/>
            <end position="1199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013915130.1"/>
        </source>
    </evidence>
    <sequence length="1363" mass="149199" checksum="9CBF49238A61C560" modified="2020-10-07" version="1">MQCRRKYSATSNGLCEEETIEKMGFWDFVEAAQRANCTCSRHKSLKPRLAVQQGPVPPAGQQPQPATKHKANEKQEKGDKPQKRPLTPFHHRVSISDKVILEADSANQRLVIPTTDSQVRFSNLRTNEVAKTPQMHPAEIASSPQPPPLSPHPCDAAEEGATKTPSTPQSQHFYQMSTPDPLVPSKAMEDRIEGLSQNFPATFPDVVEPTMYVGAAVNLEEEDADSMWKYYKVPKKKDVDFLPPPLPSEKFRDEPMGSAASDNVPSATELMLQYRKPLKVSDELIQQYQSKNQYLAAIVADQELEMDPYAFVDGDEEFPFPDKKDRPNSDREIGKKHKVSGGNNSIXIIIFEEQLSSLFSPCVSIDAQRNPPHNRTASTSLFHENDLVVSYTDLDNLFNSDEDELTPGSKRILNGTDDKSSCKESKGGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTILEGNGVTGGLQFRIEVDEGFCSPKPTEIKDYSYVYKPENSQAFMGCSMFAPLKTLPSHCLPAIKLPEECVYRQSWTVGKLDLLPPGPTMPFIKEGDGSTVDQEYGPAYTPQTHTPFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVTLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNSSGLFLEDELDILGRNSECGKEVEKRFEALRTASVEQSGSLKEADRLPDELILLLQDQCTNLFSPFGAADQDPLPKVGATGNLVRAEERDCCYDCYLALEHGRQFMDNMSGGKVDEALVKSTCLHHWSKRNVVDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRSWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGHDYDFLVLSPFALPYWERLMLEPYGSQRDIAYVVVCPENEALLNGAKNFFRDLTAVYESCKLGLHRPICKVLPDGIMRVGSTASNKLSEKSVAEWFSQTAHANKESFSRLKLYAQVCRYDLGPYLATQPLDNSLLSQSNLAPPTSHPASIQPQVAGSCTPSSTSSNAAVPMNSSSAITSATTTASSTLTTTAASSSSSSLGSAMPASKASSFPPFGNMNNAAPSSLSTQSTAVQNGQAGIQHPQQTAGITGEVLPAPTQPHPEVSESTMERDRVGIPTDGDSHAVTYPPAIVVYIVDPFTYEKKEENSSSNVWTLGLLRCFLEMIQNLPPHLRSIVAVQLVPCQYLLQPVKNEDRQIYTQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAVEMALKSPDVSTANGRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8J7</accession>
    <name>A0A6I9Y8J7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Myosin-7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106545107</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917045.1">
        <property type="nucleotide sequence ID" value="XM_014061570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8J7"/>
    <dbReference type="GeneID" id="106545107"/>
    <dbReference type="KEGG" id="tsr:106545107"/>
    <dbReference type="OrthoDB" id="312459at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000006">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000013">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.270.10">
        <property type="entry name" value="Myosin, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF69">
        <property type="entry name" value="MYOSIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin motor" evidence="17">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="245"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013917045.1"/>
        </source>
    </evidence>
    <sequence length="675" mass="77019" checksum="4177326143947936" modified="2020-10-07" version="1">MATDSAFDVLGFTAEEKVGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEATDKSAYLMGLNSADLVKGLCHPRVKVGNEYVTKGQNVQQGTLEDQIIQANPALEAFGNAKTLRNDNSSRFVSAPNLKLDLSGEGGKGKGSKKKGSSFQTVSAVHRVRGGNPRDEKDGFQLAYENLNKLMANLKTTHPHFVRCLIPNERKAPGNPVFFKAGLLGHLEELRDERLSRIITRIQARARGQLMRIEFRKIMERRIEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIITKLTKEKKSLQEAHQQALDDFQVEEDKVNSLSKAKMKLEQQVDDVSGPRPQGKEFDIHQQNSKIEDEQALASQLQKKLKELQARIEELEEEMEAERAARAKVEKQRSDLSRELEEISERLEEAGGANSVQIELNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKLELDDVASNMEQILKAKANLEKMCRTIEDQSTDHRAKFEETQRTLNDTNTQRAKLLTENGELARQLEEKEALVSQLTRGKQSYTQQMEDLKRQLEEETKAKNALAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8Z8</accession>
    <name>A0A6I9Y8Z8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermatogenesis associated 6-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SPATA6L</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917800.1">
        <property type="nucleotide sequence ID" value="XM_014062325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8Z8"/>
    <dbReference type="GeneID" id="106545687"/>
    <dbReference type="KEGG" id="tsr:106545687"/>
    <dbReference type="CTD" id="55064"/>
    <dbReference type="OrthoDB" id="5963614at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120212">
        <property type="term" value="C:sperm head-tail coupling apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032027">
        <property type="term" value="F:myosin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042769">
        <property type="entry name" value="SPATA6_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032732">
        <property type="entry name" value="SPATA6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435:SF5">
        <property type="entry name" value="SPERMATOGENESIS ASSOCIATED 6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6 SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14909">
        <property type="entry name" value="SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 6 N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="335"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013917800.1"/>
        </source>
    </evidence>
    <sequence length="420" mass="47947" checksum="051706C1FD36089B" modified="2020-10-07" version="1">MPLKVVVELQIHAVSCPGVYLTEKNDVYLTVCILGQCKETDCIPPVFPLLFHEKMWFEKVFENATDPITVVELLEKNVAKFRLSELIPSDGKELAFYETNTRYFLFPEPKLTPSYPGVDRELLMNTHPSFPGIAPKLEFSTRTTITELPLLHSKWYSNRNKVKLQRVPSTSPEKRSISPVQHKAAKSQSCKRLSRSFESRSSSLSPRRRLHEVCRDKNQPPSSLNLRSVRFKTEAAHKPPFVVRHVDASKGFSEEDQAPLSYQNARQGTITSKNPHKYQLKRAISFDSWKTTSPAAKVIREPAEDQHLNYEPSFHEARTSAATYAWKTPGLCHRSSSPNHYESTSPSGRWASPQPRLYSSSTSSWEEINERVRNLLTSPEARERLSIGATDFEIDDVLERRSISLRSSPHCDSTEQRYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBC5</accession>
    <name>A0A6I9YBC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ELOF1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921422.1">
        <property type="nucleotide sequence ID" value="XM_014065947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBC5"/>
    <dbReference type="GeneID" id="106548555"/>
    <dbReference type="KEGG" id="tsr:106548555"/>
    <dbReference type="CTD" id="84337"/>
    <dbReference type="OrthoDB" id="9049747at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921422.1"/>
        </source>
    </evidence>
    <sequence length="50" mass="5638" checksum="DE935D074D7489B8" modified="2020-10-07" version="1">TILCSRERPRNTGVISCTVCLEEFQTPITYLSEPVDVYSDWIDACEAANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCA1</accession>
    <name>A0A6I9YCA1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">von Willebrand factor C domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">VWC2L</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921752.1">
        <property type="nucleotide sequence ID" value="XM_014066277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCA1"/>
    <dbReference type="GeneID" id="106548812"/>
    <dbReference type="KEGG" id="tsr:106548812"/>
    <dbReference type="CTD" id="402117"/>
    <dbReference type="OrthoDB" id="8574072at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042979">
        <property type="entry name" value="VWC2/VWC2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057856">
        <property type="entry name" value="VWC2L_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059152">
        <property type="entry name" value="VWC2L_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46252">
        <property type="entry name" value="BRORIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46252:SF2">
        <property type="entry name" value="VON WILLEBRAND FACTOR C DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23333">
        <property type="entry name" value="VWC2L_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23334">
        <property type="entry name" value="VWC2L_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23331">
        <property type="entry name" value="VWC2L_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026715613" evidence="7">
        <location>
            <begin position="22"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="8">
        <location>
            <begin position="115"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013921752.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="24787" checksum="117ED031045B5754" modified="2020-10-07" version="1">MALHIHEACLFLLLIPSLVTPAAFNHEDYPADEGDQVSNNDNNLIFDDYRGKGCVDDSGFVYKLGERFFPGHSNCPCVCTEDGPICDQPECPKIHPKCTKVEHNGCCPECKEVKNFCEYHGKSYKILEEFKPSPCEWCRCEPSNEVHCVVADCAVPECVNPVYEPEQCCPVCKNGPNCFAGTTIIPAGIEVKVDECNICHCHHGDWWKPAQCSKRECQGKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCH2</accession>
    <name>A0A6I9YCH2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">cholesterol 7-desaturase</fullName>
            <ecNumber evidence="14">1.14.19.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106548819</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>cholesterol + NADH + O2 + H(+) = 7-dehydrocholesterol + NAD(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:51644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.19.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:51645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>cholesterol + NADPH + O2 + H(+) = 7-dehydrocholesterol + NADP(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:45024"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.19.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:45025"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Steroid hormone biosynthesis; dafachronic acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cholesterol 7-desaturase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.21" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013921760.1">
        <property type="nucleotide sequence ID" value="XM_014066285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCH2"/>
    <dbReference type="GeneID" id="106548819"/>
    <dbReference type="KEGG" id="tsr:106548819"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="UniPathway" id="UPA01020"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170056">
        <property type="term" value="F:cholesterol 7-desaturase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.380.10">
        <property type="entry name" value="Naphthalene 1,2-dioxygenase Alpha Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050584">
        <property type="entry name" value="Cholesterol_7-desaturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045605">
        <property type="entry name" value="KshA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266:SF32">
        <property type="entry name" value="CHOLESTEROL 7-DESATURASE NVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266">
        <property type="entry name" value="IRON-SULFUR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19298">
        <property type="entry name" value="KshA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Rieske" evidence="17">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013921760.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="36802" checksum="3123A915B2923905" modified="2020-10-07" version="1">MPHKSGEHFAVFRGLDGEPHVLDAYCPHLGANLAIGGRVVDNCIECPFHGWIFSGKDGRCVKIPYANKVPEFATTKVWPSCEVNDMIMVWYHCDGMSPAWQVTEQEGASSPDWTFRAATEHFVSTHIEEVPENAADVGHLIFLHKPSIMIGSDLRFTGNHRWAFFKHTWEAEWQPEPEPNRHCSVLLLTHHVLLFGKHIACLDLHVKATQVGPGIVMMALSHAIFGKGLIVQSVTPLEPLLQHVVHQIYSPRKTLSLFAKLIMWAEQIQFERDLMIWNNKKFLPNPLLVKEDAAILRHRRWFSQFYSQNSRKHNVQAGQLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE73</accession>
    <name>A0A6I9YE73_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Leucine-rich repeat-containing G-protein coupled receptor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LGR5</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922467.1">
        <property type="nucleotide sequence ID" value="XM_014066992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE73"/>
    <dbReference type="GeneID" id="106549365"/>
    <dbReference type="KEGG" id="tsr:106549365"/>
    <dbReference type="CTD" id="8549"/>
    <dbReference type="OrthoDB" id="1883493at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000028">
        <property type="entry name" value="leucine-rich repeat-containing G-protein coupled receptor 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000770">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF71">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027080485" evidence="18">
        <location>
            <begin position="29"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="557"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="594"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="638"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="680"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="721"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="767"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="802"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="573"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013922467.1"/>
        </source>
    </evidence>
    <sequence length="907" mass="100927" checksum="5AAC3B149D951026" modified="2020-10-07" version="1">MDTAAVVAVPVRALLFLSLLGLVGSGLGTRQRSCPGFCHCEPDGMLLRVDCADLGLADIPANLSVFTSYLDLSMNNISKLPLNSLHKLRFLEELRLAGNSLTYIPRGALAGLFRLKVLMLQNNLLRQVPSEALQNLHSLQSLRLDANHISFIPSNCFSGLMSLRYLWLDDNSLTEIPVQAFRSLPTLQAMTLALNKIYYIPDYAFGNLSNLVVLHLHNNRINFLGKKCFEGLHSLETLDLNYNNLNEFPTSVNVLRNLKELGFHNNNIRSIPEQAFMGNPSLTAIHFYDNPIQTVGKSAFHHLPELRTLTLNGVSEMTEFPDLTGATSLESLILTGAQITTLPESICEHLPNLQVLDLSYNHLEELPSFTACEKLQKIVLHHNEIYEIHAGTFQQLVALHSLDLAWNKISIIHANAFSSLLSLIKMDLSSNHLSSFPITGLHGLTHLKLTGNYPLQSLISSENFPELKVIEMPYTYQCCAFRAHDNYYKIQNQLNKAENGSADEFQRKDSSSFHINDEHAFEDFSLDFNEDLKTYHSVRCSPSPGPFKPCDCLFGSWLIRVGVWTLILLAFICNILVIATVFQVPLFISSIKFIIGLIAIVNTLMVVSSSVLASVDAITYGVFAQYGTWWEDGIVCHIIGILSIFASEASIFLLTLATVERVFSVKYRTKFETKHTLTNIKRAIIFCFVLALIIAVIPVLSDSEYGGCPLCIPLPFGRPSGVGYTVAMVLMNSFCFLVMTAAYTKLYCSLDKGEMDIILDCSMIKHIALLLFINCILYCPVAILSFSSLLNLHFIGPEVIKPILLVIVPLPACLNPLLYILFNPHFRKDLRRLQKLSFGWKTSKHPSLVSVNSEDAEKQSCNSTQALVTFASARISCNVVPSKPAVPSSCEFSESCNLSSITFVPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFC5</accession>
    <name>A0A6I9YFC5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Clustered mitochondria protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CLUH</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922872.1">
        <property type="nucleotide sequence ID" value="XM_014067397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFC5"/>
    <dbReference type="GeneID" id="106549691"/>
    <dbReference type="KEGG" id="tsr:106549691"/>
    <dbReference type="CTD" id="23277"/>
    <dbReference type="OrthoDB" id="1414216at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2280.10:FF:000001">
        <property type="entry name" value="Clustered mitochondria (CluA/CLU1) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028275">
        <property type="entry name" value="CLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF6">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clu" evidence="3">
        <location>
            <begin position="326"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="627"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013922872.1"/>
        </source>
    </evidence>
    <sequence length="906" mass="102374" checksum="E609A6DD6DB00004" modified="2020-10-07" version="1">MNGDSLHENGKEEARQNDHEDPNHTDDQEVIVIQDTGFTVKVHAPAVEPFPLQVSPQEMVQEIHQVLMDREDTCHRTCFSLQLDGNVLDNFAELKTIEGLQEDSVLKVVEEPYTVREARIHVRHVRDLLKSLDPSDAFNGVDCNSLSFLCVFTDGDLGESGKRKKKGTELEQIDCTPPEHILPGSKERPLCPLQPQNRDWKPLQCLKVLTMSGWNPPPGNRKMHGDLMYLYVITVEDRHVSITASTRGFYLNQSTAYNFNPKPANPSFLSHSLVELLNQISPTFKRNFAALQKKRVQRHPLERISTPFQVYTWTAPLAEHAMDCVRAEDAYTSRLGYEEHIPGQTRDWNEELQTTRELPRKNLPERLLRERAIFKVHSDFTAAATRGAMAVIDGNVMAINPSEETKMQMFIWNNIFFSLGFDVRDHYKDFGGDVAAYVAPTNDLNGVRTYNAVDIEGLYTLGTVVVDYRGYRVTAQSIIPGILEREQEQSVIYGSIDFGKTVVSHPKYLELLEKTSRPLKIQKHLVLNDKSEEVELCSSVECKGIIGNDGRHYILDLLRTFPPDLNFLPVEGEDMPEECQKMGFPKQHRHKLCCLRQELVDAFVEHRYLMFMKLAALQLMQQKANKPSSPSAGNGNSSPTTFPENGVEDKETPLLEVSKMEDNTPGLEQVKELTETIASDDGAVDPKSREVIQNACKAVGSISNTAFDIRFNPDIFSPGVRFPESSKEQIQDQKRLLKDAAAFLVSYQIPGLVKDCLDHTMLPMDGTTLAEAMHQRGINMRYLGKVVTFILETPAQNQLDHIYKIGITELITRSAKHIFKIYLQGVELSGLSAAISHFLNCFLSSFPNPVGHLPADELVSRKKNKRRKNRNLGTADNTAWAAMSPQELWKNICSEAKSYFDFGLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHE6</accession>
    <name>A0A6I9YHE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydrothymine dehydrogenase</fullName>
            <ecNumber evidence="5">1.3.1.2</ecNumber>
            <ecNumber evidence="4">1.4.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Dihydrouracil dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DPYD</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; beta-alanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dihydropyrimidine dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.2" evidence="5"/>
    <dbReference type="EC" id="1.4.3.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013923607.1">
        <property type="nucleotide sequence ID" value="XM_014068132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHE6"/>
    <dbReference type="GeneID" id="106550261"/>
    <dbReference type="KEGG" id="tsr:106550261"/>
    <dbReference type="CTD" id="1806"/>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00131"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017113">
        <property type="term" value="F:dihydropyrimidine dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002058">
        <property type="term" value="F:uracil binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019483">
        <property type="term" value="P:beta-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006212">
        <property type="term" value="P:uracil catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02940">
        <property type="entry name" value="DHPD_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000023">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000061">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073:SF2">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="569"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="603"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923607.1"/>
        </source>
    </evidence>
    <sequence length="650" mass="70599" checksum="D2833FD4CC3591CB" modified="2020-10-07" version="1">MELAKEEKCEFLPFLSPQKVVIKGGKIVAMEFLRTEQDEDGNWKEDKEQTVRLRADIVISAFGSTLNDPKVKGALHPLQFNRWGLPEVDGETMQTSEPGVFAGGDISGLTNTTVESVNDGKQASWYMHKYIQSLHGALVPAVPQLPLFYTPIDLVDISIEMAGLRFLNPFGLASATPTTSSTMIRRAFEAGWAFALTKTFSLDKDIVTNVSPRIIRGATTGPMYGPGQGSFLNIELISEKTAAYWCRSISELKSDFPDKIVIASIMCSYNKNDWTELSKMAEVSGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVQIPFFAKLTPNVTNIVNIARAAQEGNADGVTATNTVSGLMGLKAGGIPWPSVGRSKKTTYGGVSGNAIRPIALRAVSAIAKALPGFPILATGGIDSAESGLQFLHSGASVLQVCSAIQNQDYTVIDDYCTGLKALLYLESLEELQDWEGQSPPSIQHQKGKPVPKISELIGKKLPSFGPYLEQRKKIIAENKLKLKAQNSSLHSQLERKCFAPKKPIPAVKDVIGKALQYIGTYGELSSTEQVVALIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVTDSCTGCTLCLSICPIIDCIRMVSRTTPYEAKRGLPLAVIPVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHX6</accession>
    <name>A0A6I9YHX6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rap1 GTPase-activating protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550165</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923460.1">
        <property type="nucleotide sequence ID" value="XM_014067985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHX6"/>
    <dbReference type="GeneID" id="106550165"/>
    <dbReference type="KEGG" id="tsr:106550165"/>
    <dbReference type="OrthoDB" id="2499658at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF3">
        <property type="entry name" value="RAP1 GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923460.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="43287" checksum="CC89AA4F0FF1E4EA" modified="2020-10-07" version="1">MFHVSTKLPYTEGDAQQLQRKRHIGNDIVAIVFQDENTPFVPDMIASNFLHAYVVVQAENACSDKTLYKVSVTARDDVPFFGPPLPNPAVFQKGPDFQAFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHLNSQSMMGLGAEEDKMENGGGGSSSFFESFKRVIRSRSQSMDAMGLSSKKQSTVTTSHSGSFGHPSPDIAKALGLSLIVPGKSPTRKKSGPFGSRRSSAIGIENIQEVQEKRESPTGSAKTPDGSHPGQEPRSEASSNQSSPEMPTTKNRADPVVPKPEAGHESSRSSSSASSFGSMVEDTEGCRDDTSGESPFPSTHSNKRDSFMYSTWLDDSTSTPSGGSSPGPSRSPQPDPRKPTDPPCPEIQIQLEHSDQQPPHLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIG9</accession>
    <name>A0A6I9YIG9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Programmed cell death protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PDCD5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May function in the process of apoptosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924168.1">
        <property type="nucleotide sequence ID" value="XM_014068693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIG9"/>
    <dbReference type="GeneID" id="106550736"/>
    <dbReference type="KEGG" id="tsr:106550736"/>
    <dbReference type="CTD" id="9141"/>
    <dbReference type="OrthoDB" id="10252486at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.140:FF:000001">
        <property type="entry name" value="Programmed cell death protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00071574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924168.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="14080" checksum="C4C16EDE7B028432" modified="2020-10-07" version="1">MADEELEALRKQRMAELQAKHGDPSSEIAQQESKQREAEMRNNILAQVLDQAARARLSNLALVKPEKAKAVENYLIQMARFGQLGGKVSEQGLIEILEKVSQQMDKKTTVKFNRRKVLDSDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YII5</accession>
    <name>A0A6I9YII5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RAS protein activator like-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923754.1">
        <property type="nucleotide sequence ID" value="XM_014068279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YII5"/>
    <dbReference type="GeneID" id="106550377"/>
    <dbReference type="KEGG" id="tsr:106550377"/>
    <dbReference type="OrthoDB" id="5572587at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013923754.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="39010" checksum="7B174951B5BDF794" modified="2020-10-07" version="1">MQWIENRSAFGQIETNFYHQSGQVLYVGPLCSSVYKGTQEKLQPLPAILQAIQEGTPVPVSVLPGPHMEERDAEKQKLGFVPPRDLAKYSPLIKSQSMTSIQKNRGKEDALTPLHPTKSRSKVQRTQSVPTQSKASRRLRKQRSIGRVDDCQDSNPQPYDSSLCNAGSHTASQGCSKLHLSVSLPQKPAVPWLRHSEDASAIKNCLYPMQPLEQYARQMEVLQMKLSSATDKQKLLEEQVKSLSVQNQALLQEQARFQERGEILGKRLEETELCLVQLSGRVSCIEAGWKKDHEKLQVSEEKAKELDLQLSRMERDHDQRFHAASQMLGCRDNQPHYSNNSQGLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJP1</accession>
    <name>A0A6I9YJP1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Growth/differentiation factor 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GDF11</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924386.1">
        <property type="nucleotide sequence ID" value="XM_014068911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJP1"/>
    <dbReference type="GeneID" id="106550922"/>
    <dbReference type="KEGG" id="tsr:106550922"/>
    <dbReference type="CTD" id="10220"/>
    <dbReference type="OrthoDB" id="5948587at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19388">
        <property type="entry name" value="TGF_beta_GDF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000003">
        <property type="entry name" value="Growth differentiation factor 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000006">
        <property type="entry name" value="growth/differentiation factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF166">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="10">
        <location>
            <begin position="214"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013924386.1"/>
        </source>
    </evidence>
    <sequence length="326" mass="37644" checksum="46EE28F8EEF3197E" modified="2020-10-07" version="1">MRLESIKSQILSKLRLKEAPNITREVVNQLLPKAPPLQQILDLHDFQGDSFQHDDYLEEDEYHATTETVISMAQETDPVVQIEGNPHCCFFNFSPKIMFTKVVKAQLWVYLRPVQHTSTVYLQILRLKPVTEDGSRHIRIRSLKIDLNSRIGHWQSIDFKHVLQNWFKQPQNNWGIEINAFDPNGNDLAVTSLGPGAEGLHPFMELRVLENNKRSRRNLGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJX4</accession>
    <name>A0A6I9YJX4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Major histocompatibility complex class I-related gene protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106550851</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924309.1">
        <property type="nucleotide sequence ID" value="XM_014068834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJX4"/>
    <dbReference type="GeneID" id="106550851"/>
    <dbReference type="KEGG" id="tsr:106550851"/>
    <dbReference type="OrthoDB" id="8936120at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF242">
        <property type="entry name" value="MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-RELATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0490">MHC I</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="102"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924309.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="28722" checksum="4735408960051FEC" modified="2020-10-07" version="1">MLQRRYNQSGGYHIVQMIIFCKVEEDGKRSGHWQYGYDGRDFLYYNMDTSTWIAADMEAQEIKRKWERETAIKQRFTAYMESTCMEWLQKNDRYGLKLRKEPPVVTMSSRTEVEDGMETHICCMDGFYPRDIDATWRRDGEVWLEETFHTSVAPNADGTYHYWMSIQIDPKERSRYRCHVEHDGLQEPLDMELKVPGSKSNLWLIIGSVVGVLVACGVAGILLCLKFKKGKDEPRREPGHEPVTSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJY1</accession>
    <name>A0A6I9YJY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hemopexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">HPX</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the hemopexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924319.1">
        <property type="nucleotide sequence ID" value="XM_014068844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJY1"/>
    <dbReference type="GeneID" id="106550861"/>
    <dbReference type="KEGG" id="tsr:106550861"/>
    <dbReference type="CTD" id="3263"/>
    <dbReference type="OrthoDB" id="8953614at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000009">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051298">
        <property type="entry name" value="Heme_transport/Cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22917:SF9">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22917">
        <property type="entry name" value="HEMOPEXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027087839" description="Hemopexin" evidence="16">
        <location>
            <begin position="26"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="14">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="14">
        <location>
            <begin position="97"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="14">
        <location>
            <begin position="196"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="14">
        <location>
            <begin position="265"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="14">
        <location>
            <begin position="311"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013924319.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="52400" checksum="22F6E3D6E6EE36F7" modified="2020-10-07" version="1">MGVLSAALCLSWALVLVGTHPLGHGGHGNKTAGTWHHLGSEPPANSTELIQRCADEEGFDAVTLDEMGTMVFFKGSLVWKGFIGTPQPIEDSWPEIRGPVDAALRIHYHNHPDGLHDSVLLFQGKKVWAYHEGRLRLGYPKWIEQEFPGIPADLDAAVECHPKECPSETIVFFKAPNVFTYDLRTKAVKRRDWPAVSNCTAAVRWLERYYCFQGIRFLRFDPLRGDVPPHYPLDARDYFMRCPGRGHGRESRRNSTLWSTFNACSGRPFPAFSSDDPGRIYAFRGGHYFRVDSVRDGLHAWLLNHTWSGLEGEVDAAFSWADKLYFIQGSQVLIYRAEHGYSRVEGYPRPLVEELGVSQADAAFTCPHSSELFVIQGNRIQPIDLLQSPRRPGPSHLLPHPRVDSAICTTKGVFLFVGADFHQYPDVAQLATATIPAPAQNTAAVFFRCSPSGAPPHPPRGGQRPGDQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK91</accession>
    <name>A0A6I9YK91_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Keratin, type I cytoskeletal 47 kDa-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106551083</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924571.1">
        <property type="nucleotide sequence ID" value="XM_014069096.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551083"/>
    <dbReference type="KEGG" id="tsr:106551083"/>
    <dbReference type="OrthoDB" id="9119337at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF180">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="4">
        <location>
            <begin position="63"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924571.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="19265" checksum="4DA95D440AB366D6" modified="2020-10-07" version="1">MPTTFSTHSMSSGSMQGPRGRRLLALSSSQAVPSIHRARGDMAKLSYGDGQAFMDTNILHMNEKGTMKNLNDRLAAYLDKVLSLEKSNMQLEQNIQEYYTKQASTGTPDMSAYFNEMTQLKSEEAKVLVNGVVTQNFNIRKGTTQGNSGANLENFLPGLLAHLIQSPPASXHTRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKB4</accession>
    <name>A0A6I9YKB4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rhophilin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RHPN2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924190.1">
        <property type="nucleotide sequence ID" value="XM_014068715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKB4"/>
    <dbReference type="GeneID" id="106550755"/>
    <dbReference type="KEGG" id="tsr:106550755"/>
    <dbReference type="CTD" id="85415"/>
    <dbReference type="OrthoDB" id="64867at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051497">
        <property type="term" value="P:negative regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.280:FF:000003">
        <property type="entry name" value="RHPN1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047138">
        <property type="entry name" value="RHPN1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23031">
        <property type="entry name" value="RHOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23031:SF5">
        <property type="entry name" value="RHOPHILIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="1">
        <location>
            <begin position="74"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924190.1"/>
        </source>
    </evidence>
    <sequence length="517" mass="58708" checksum="B101C3388C007D04" modified="2020-10-07" version="1">MVRLILLLNDCSLNIFILFYFSFGSASTSPKVRQQVLLELSYVNSNLQILKEELEGLNISVEVYQNMEESFSIPLIPLGLKETKDVDFAGPLKDFILEHYSEDGTEYENEIADLMDLRQACRTPSRDESGVEMLMSYYVQLGFVDNRFFSPTRNPGILYTWYDSFTGVPVCQKNLLLEKASILFNIGALYTQIGTKCNRQTGAGLQKAISAFQKAAGVLNYLKETFTHTPSYDMSPAMLSVLVKMMLAQAQECIFEQICLPGIRNEFFTLIKMAQEVAKVGEMYMLVDTAMSQAPVKENIPYSWSKLAEVKSDHFRALAHYFVATMLNDHQIHQTDDEDQQEKAFCQLYDHMPEGMTPLAVLRDRSQRKQLGRLHLHKALLYHKEALRVCSLCTKLRNIEILQEILSVAHKRTLLKYTQQEQKDDFFSLVPAPNILSHTNHKLELIPPLFSNVKVRDFFERLGPPAVFSAKQRWSPPRAIRFNCEDGLLGFTLKGGSPVQIDCIDPASPAALAGLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKT9</accession>
    <name>A0A6I9YKT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Selection and upkeep of intraepithelial T-cells protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106551230</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SKINT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924761.1">
        <property type="nucleotide sequence ID" value="XM_014069286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKT9"/>
    <dbReference type="GeneID" id="106551230"/>
    <dbReference type="KEGG" id="tsr:106551230"/>
    <dbReference type="OrthoDB" id="9986391at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903037">
        <property type="term" value="P:regulation of leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050863">
        <property type="term" value="P:regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042110">
        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05713">
        <property type="entry name" value="IgV_MOG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000088">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF149">
        <property type="entry name" value="BG-LIKE ANTIGEN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="43"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="163"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013924761.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="40277" checksum="E75D5C4E9C121F5B" modified="2020-10-07" version="1">MCLEDQNKKTREVKTFSCVSKMNSFLFIFTTFLVILPVDNGNPMQLTLLGPHHPVTASLRETIVLHCHLSPRTNVQNMEITWFRSTNSSYVHLYRSGKDHLERQQPEYQGRTEFLKDGIGDGKIGLKIFNISLFDEGHYHCSVKNGSFQQEATLDMKVTASGPAPLLSIEGYQDRGIRVVCRSSGWYPKPEILWRELSGNLLSSLAKKDSQRNDGTFDVQADIILKGSSNLTCVIRNSLLDLEKESTVHITNHLFPKMSSWIVALCFSLMAMVGFAIFTFYLLNKYRKLVTELNWRNMVIPIEKGWWLSCLLSLQNVNSIIIELICETEYFTSRFYSILQRIFLNSKVFMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLK7</accession>
    <name>A0A6I9YLK7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box only protein 18-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106551345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924914.1">
        <property type="nucleotide sequence ID" value="XM_014069439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLK7"/>
    <dbReference type="GeneID" id="106551345"/>
    <dbReference type="KEGG" id="tsr:106551345"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013924914.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="14131" checksum="105585744874886A" modified="2020-10-07" version="1">MINSTFPLSFCRSYKHKLNLGSLTSYLVSYVLSNRKGQSPFIRAKTVVQTLAAFFASADAAITVEHTPIWCKNNKGINVLVEEREKHIIVEEAKQIWAKMKMRSPTTDMAHRMTHDGRFLGFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLS2</accession>
    <name>A0A6I9YLS2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="36">Multidrug resistance-associated protein 4</fullName>
            <ecNumber evidence="4">7.6.2.2</ecNumber>
            <ecNumber evidence="15">7.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="41" type="primary">ABCC4</name>
    </gene>
    <organism evidence="40 41">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>3',5'-cyclic AMP(in) + ATP + H2O = 3',5'-cyclic AMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:66185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>3',5'-cyclic GMP(in) + ATP + H2O = 3',5'-cyclic GMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:66189"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>an S-substituted glutathione(in) + ATP + H2O = an S-substituted glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19121"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:19122"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>cholate(in) + glutathione(in) + ATP + H2O = cholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29747"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:66397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dehydroepiandrosterone 3-sulfate(in) + ATP + H2O = dehydroepiandrosterone 3-sulfate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61364"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:61365"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>glycochenodeoxycholate(in) + glutathione(in) + ATP + H2O = glycochenodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36252"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:66409"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66400"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29746"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:66401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>glycodeoxycholate(in) + glutathione(in) + ATP + H2O = glycodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66380"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:82982"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:66381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>glycoursodeoxycholate(in) + glutathione(in) + ATP + H2O = glycoursodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:132030"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:66417"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>leukotriene B4(in) + ATP + H2O = leukotriene B4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57461"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>prostaglandin E1(in) + ATP + H2O = prostaglandin E1(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57397"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:66393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>prostaglandin E2(in) + ATP + H2O = prostaglandin E2(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:66389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>taurochenodeoxycholate(in) + glutathione(in) + ATP + H2O = taurochenodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66412"/>
            <dbReference type="ChEBI" id="CHEBI:9407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:66413"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>taurocholate(in) + glutathione(in) + ATP + H2O = taurocholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66404"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36257"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:66405"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>tauroursodeoxycholate(in) + glutathione(in) + ATP + H2O = tauroursodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:132028"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:66421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>urate(in) + ATP + H2O = urate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16461"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:16462"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="35">Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); this interaction accelerates MRP4 internalization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="4"/>
    <dbReference type="EC" id="7.6.2.3" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013924730.1">
        <property type="nucleotide sequence ID" value="XM_014069255.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551206"/>
    <dbReference type="KEGG" id="tsr:106551206"/>
    <dbReference type="CTD" id="10257"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18593">
        <property type="entry name" value="ABC_6TM_MRP4_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18601">
        <property type="entry name" value="ABC_6TM_MRP4_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000027">
        <property type="entry name" value="ATP-binding cassette subfamily C member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000973">
        <property type="entry name" value="Multidrug resistance-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000014">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030240">
        <property type="entry name" value="ABCC4_TMD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047083">
        <property type="entry name" value="ABCC4_TMD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF357">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14 37" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="40" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 37" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 37" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="732"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="829"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="913"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="37">
        <location>
            <begin position="941"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="39">
        <location>
            <begin position="94"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="38">
        <location>
            <begin position="414"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="39">
        <location>
            <begin position="678"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="38">
        <location>
            <begin position="1006"/>
            <end position="1239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00051304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00051604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00051624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00051844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00052534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00052647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00052963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00062847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00082792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="RefSeq" id="XP_013924730.1"/>
        </source>
    </evidence>
    <sequence length="1290" mass="146063" checksum="73EAA14C58075D24" modified="2020-10-07" version="1">MEALPSRDEKANPSWDANFCSRLFFCWLNPLFVIGHKRKLEEDDMYKVLTEDSSKVLGETLQWYWDKEVQKAEKEARTPRLTKAIILCYWKSYLMLGCFTLVEETLILIQPVFLGMIIGYFEKIGSTHADENALAYAYVSAAALSVCTLILAITHHLYFYHVQRAGMKLRVAMCHMIYHKALRLSNTAMSKTTTGQIVNLLSNDVNKFDQVTIFLHFLWAGPVQAIAVTVLLWLEIGPSCLAGMVVLFILLPFQTCIGRLFSALRSRTAAFTDARIRTMNEVITGMRIIKMYAWEKSFAELVSNMRRKEISVVLKSSYLRGMNLASFFIASKITVFMTFMTYVLLGNRISASRVFVAVSLYGAVRLTVTLFFPAAIEKVSETLVSIRRIKNFLILDEVPLFKPQLKNNIENPFLNLHDLTCAWDKTMEMATLQDISLSVKPGELLTVIGPVGAGKSSLLSAVLGELSPIKGFIDVQGKIAYVSQQPWVFSGTVRNNILFGKEYRKEKYEKVLRACALKKDIDQLEDGDLTMVGDRGATLSGGQKARINLARAVYQDADIYLLDDPLSAVDAEVSRHLFESCAQIESKNSACVKSRSLEALQKVVLFYCMFETSLYMFNNIQVVSTVKAIPKSLLLFKIDLEMGPLLTALPEESRSEGKIGLTIYKKYFSAGANYFVTFILLVLNILAQVTYVLQDWWLSYWANQQVMINVTRMEYGGQNITENLDLPWYLGIYAGLTVATILFSILRSLLMFHILVNAARTLHNKMFQSVLRTPVLFFDRNPIGRILNRFSKDIGHLDDLLPLTFLDFVQTFLQVCGVVGVSIAVIPWILIPLVPLLIVFIFLRRYFLDTSRDIKRLESTTRSPVFSHLSSSLQGLWTIRAFQAQQRFQEIFDAHQDLHTEAWFLFLTTSRWFAVRLDAICAIFVIVMAFGSLLLAQTLDAGKVGLALSYAITLTGMFQWGVRQSAEVENLMISVERVTEYTELEQEAPWHTYKRPPAEWPTEGVIAFDDVNFAYSADGPLVLKHLSVLIKSKEKVGIVGRTGAGKSSLIAALFRLAEPQGRIWIDKYLTSELGLHDLRKKISIIPQEPVLFTGTMRKNLDPFNEYSDEDLWNSLEEVQLKEAIEDLPDKLETQLAESGSNFSVGQRQLVCLARAILKKNKILIIDEATANVDPRTDELIQKTIREKFAQCTVLTIAHRLNTIIDSDRIMVLDRGRLKEYDEPYILLQEKESLFYKMVQQLGKAEALSIVETAKQVYFKKNYPEVTSSDRAVTSSSTSESQGLIIFETAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YM61</accession>
    <name>A0A6I9YM61_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Retinaldehyde-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RLBP1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925317.1">
        <property type="nucleotide sequence ID" value="XM_014069842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YM61"/>
    <dbReference type="GeneID" id="106551696"/>
    <dbReference type="KEGG" id="tsr:106551696"/>
    <dbReference type="CTD" id="6017"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF200">
        <property type="entry name" value="RETINALDEHYDE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL/TRIO N-terminal" evidence="3">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925317.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="23143" checksum="699136F6948CDC68" modified="2020-10-07" version="1">MTTIASPLLAGAQANHQSTGTFRPVSEEEQALRTKLERLSTSDHGPVFGKHEKIPEHTLQKAKDELNETSEKRELAIKELRELIQEKAKDGDDICKTVADKMQEKEDAFLLRFIRARKFDLNRAYDLIKGYVNFRQQYPELFENLVPEAVRSTIEAGYPGILCNRDKYGRVVLLFNIENWDYEEITFDEVGICSVKAFIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YME7</accession>
    <name>A0A6I9YME7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">adenylate cyclase</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ADCY9</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013925164.1">
        <property type="nucleotide sequence ID" value="XM_014069689.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551564"/>
    <dbReference type="KEGG" id="tsr:106551564"/>
    <dbReference type="CTD" id="115"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000008">
        <property type="entry name" value="Adenylate cyclase type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF8">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="686"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="733"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="759"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="789"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="844"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="18">
        <location>
            <begin position="267"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="18">
        <location>
            <begin position="925"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="520"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1184"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1184"/>
            <end position="1196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013925164.1"/>
        </source>
    </evidence>
    <sequence length="1216" mass="135558" checksum="A6B5647B4B950B6C" modified="2020-10-07" version="1">MINYLLGLDATPHQLYTGAEPTTSEFTVIMHGAKVKTIEGIVMAADSARSFSPLEKFGQNFLEKLIGIEIPHKLLERVTFVDTPGIIENRKQQEWLXVAALLLLYAVPHLPLYLSLLLGLGGSALFEALGYGFRDERCFALPAGERLAAWELLGKALLHLCIHATGVHLFIMSEVRSRSTFLKVGQSIMHGKDLEVEKALKERMIHSVMPRIIADDLMKQGDEDSENSSSAKRHSASSPKSRKKKPSIQKTPIIFRPFKMQQIEQVSILFADIVGFTKMSANKSAHALVGLLNDLFGRFDRLCEDTKCEKISTLGDCYYCVAGCPEPRADHAYCCIEMGLGMIKAIEQFCQEKKEMVNMRVGVHTGTVLCGILGMRRFKFDVWSNDVNLANLMEQLGVAGKVHISEATAKYLDDRYEMEDGKVMERVGQSVVADQLKGLKTYLISGLKKKETHCSCSETLLLPGLEAGDGSKLQGALSENMDDLAKTFKYLEKNKICHSCSVTFSPGYAVSAEEGAIQNGCQEDHKTSTKSPGGGSPKSRNGLLSPPQEEKLSNSQTSLYEMLQEKGRWVGASLDHSALLPLRFKNIREKTDAHFVDVIKEDSLMKDYFFKPPINKLSLNFLEHDLEMAYRSSYQEEVIRRAPVKTFASATFSSLLDVSLSATVFLILSIACFLKHGLVASPPPPAAVAVAVTAVLLEVVSLTVSIRMAFFLEDMMLCTRRLLEVIAGWLPRHFIGAVLVSLPALAVFSHFTSEFGTDLYSTMFMCSAVLITIVQYCNFCQLSSWMRSSLATLVGGVLLLLLYVSWCPDSDLEDSSLLSLRNLSFIENACNRSASEDFRRPADLIGLEVVLGLFLLLVLVWFLNREFEVSYRLHYHGDVEADLHRTKIQSMRDQADWLLRNIIPYHVAEQLKVSQSYSKNHDSGGVIFASIVNFSEFYEENYEGGKECYRVLNELIGDFDELLSKPEYSNIEKIKTIGATYMAASGLNASQCQDSSNPHAHLQTLFEFAKEMMRVVDDFNNNMLWFNFKLRVGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDTTGVECRIQVSEESYRILHKMGYDFDYRGTVNVKGKGQMKTFLYPKCMESGVVPHHQLSISPDIRVQVDGSIGRSPTDEITNLVPSVQNLDKVPPDTELKDLLPRFKKLPKELVKLDSRPQFDAAGEKGGCEEAGPGEANELTKLNSSWSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMT5</accession>
    <name>A0A6I9YMT5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LisH domain and HEAT repeat-containing protein KIAA1468 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KIAA1468</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925446.1">
        <property type="nucleotide sequence ID" value="XM_014069971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMT5"/>
    <dbReference type="GeneID" id="106551815"/>
    <dbReference type="KEGG" id="tsr:106551815"/>
    <dbReference type="CTD" id="57614"/>
    <dbReference type="OrthoDB" id="1695393at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040362">
        <property type="entry name" value="RELCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32059">
        <property type="entry name" value="RAB11-BINDING PROTEIN RELCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32059:SF0">
        <property type="entry name" value="RAB11-BINDING PROTEIN RELCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="HEAT" evidence="1">
        <location>
            <begin position="825"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="177"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925446.1"/>
        </source>
    </evidence>
    <sequence length="1037" mass="115683" checksum="F6E6D6F16BC9B749" modified="2020-10-07" version="1">MAAAGGGGVNPFLSDSEESEPEESRPGGSSSSDPGASAAPLPVSSSVSSSPPFQPLLPPPPPSQAEEPPRVPVDALAAQLLRDQFVLTALELHAELLESGRELPRLRDYFSNPGNFERQSAGGAGALAPTPSASGAPPGMIAMVGGGKEPGAGQLHRAGSISTLDSLDFARYSDDGNRETDERVAVLEFELRKAKETIQALRANLTQAAENEVPLQERRNYKSSPEIQEPIKPLEKRALNFLVNEFLLKNGCKLTSITFSDENDDQDFELWDDVGLNIPKPPDLLQLYRDFGNHQVIAKDIVDVACGPEEEELEATTPILENIPVFGTSESIEQCVLVQKLEDQINALSTEKWSLMEQIQRLESEIDYLRSQKTSIPMVCDTVQPSSAQVPPTLPDDNGKYLDIKSSTNDDKLGRTEEEITLMEPECRTSKEDIGIPFAYKEREKFPFCSPSNKAAIHFDKPNRKLSPAFHQALLSFCQMSAESRLGSEVSRIADSEKSVMLMLGRCLPHIVPNVLLAKREELIPLILCTACLHPEPKERDQLLHILFNLIKRPDDEQRNFYTYFSFHCQGFELLLSALGDPSERVVSATHQVFLPAYAAWTTELGNLQLHLIPTLLIKIEKLLKESEHGLDEHKLHMYLSALQSLIPSLFALVLQDAPFTSKAKLQGEVPHIEEDRKLLVGFLEDVMTMLSLSHAPLDSLKASFVELGLPQLIEIVGKINVASTACVHEFSRFFWRLCRTFGKIFTNTKVKPQFQEILRLSEENIGRYIFPNHQFYANPAYHELLLTVLWYGVVHTSALVRCTAARMFELTLRGMREALVDKRVAPALVTLSSDPEFSVRIATIPAFGTIMETVTQRELLERVKMQLASFLEDPQYQDQHSLHTEIIKTFGRVGPNAEPRFRDEFVLPHLHKLSLVNNQQSADSKRLDIATHLFEAYSALSCCFISEELMISHFLPGLRCLRNDMEALSPEHEVILNSMIKECEQKVENRNVQEPQGSMSIAASLVSEDTKTKFLNKMGQLTTSGAMLANVFQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNQ5</accession>
    <name>A0A6I9YNQ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 4 regulatory subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PPP4R4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative regulatory subunit of serine/threonine-protein phosphatase 4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925907.1">
        <property type="nucleotide sequence ID" value="XM_014070432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNQ5"/>
    <dbReference type="GeneID" id="106552213"/>
    <dbReference type="KEGG" id="tsr:106552213"/>
    <dbReference type="CTD" id="57718"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008287">
        <property type="term" value="C:protein serine/threonine phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000097">
        <property type="entry name" value="Serine/threonine-protein phosphatase 4 regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039918">
        <property type="entry name" value="PPP4R4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4 PPP4R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="214"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="253"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="714"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="816"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="730"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="741"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="816"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="863"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013925907.1"/>
        </source>
    </evidence>
    <sequence length="874" mass="99980" checksum="37A37D40008017FD" modified="2020-10-07" version="1">MHPIIIFSYKMDFSQNNLFGYIEDLQELTIIERPVRRSLKTPEEIERLTVDEELNDIERAVYLLSSGQDVQGTSVVANLPVLMRQNPTETLRRVLPKVREVLPVASVEMQLTAAVSLLTILQEESISIHTYSHSFLQLILQNLEHRDAGVSNAWLDTLLAAIEALPKETIRHEILNPLVSKAQLSQTLQSRLVSCKILGKLANKFEAHIIKREILPLVKSLCQDVEYEVRSYMCRQLELIAQGIGTELTKNVVLPELVELARDEGSSVRLAAFETLVNLLEMFDADDRSQTVLPLVKSFCEKSFKADESILVSLSFHLGKLCNGLYGIFTPEQHLRFLEFYKKLCTLGLEQENGHNDNQIQLQTLEQEKKYISVRKNCAYNLPAMMIFVDPKNFNLELYSTFFCFCHDPEVPVRHTMAGSFYEVAKLLNTDVYIIHKELIALLQDESVEVIDALINHLPEILELLSTGGENSGSENKLLNFPELIPALTRAEQRAATSLKWRTHEKLLQKYACLPHIISSDQIYYRFLHRMLTIIMTNNVLPVQRAAARTLCIYLRYNRKQEQRYEVIQKLIEQLGQGKSYWNRLRFLDTCEFIIELFSKSFFCEYFFLPVLELTHDPVANVRMKLCYLLPKVKSTLKVPTDKHLLQQLEMSVRKLLCEEKDKDVLNIVKKMVLELDRMDTSLDVFQKRFYENDLLDQQKEREEQLLLEMEQLEKEKQQNESRSTNMSDKIYEKKRRDSKGASTLAKTIPMSVSGNPSGASASKEVKKSKLVRSQSFSSQALQSKYGNIDKCPSKSSSLGYTSVSGVAKNSMFSFTDDSFRTRPTNVSGTAAFNSTSTLPSSSRNTNNTVDQKSNGNKESHSRKVSLKNRKSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP86</accession>
    <name>A0A6I9YP86_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16 17">RGM domain family member B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16 17" type="primary">RGMB</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926302.1">
        <property type="nucleotide sequence ID" value="XM_014070827.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926303.1">
        <property type="nucleotide sequence ID" value="XM_014070828.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926304.1">
        <property type="nucleotide sequence ID" value="XM_014070829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP86"/>
    <dbReference type="GeneID" id="106552526"/>
    <dbReference type="KEGG" id="tsr:106552526"/>
    <dbReference type="CTD" id="285704"/>
    <dbReference type="OrthoDB" id="10013795at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.10:FF:000001">
        <property type="entry name" value="Repulsive guidance molecule BMP co-receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010536">
        <property type="entry name" value="RGM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF5">
        <property type="entry name" value="REPULSIVE GUIDANCE MOLECULE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06535">
        <property type="entry name" value="RGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Repulsive guidance molecule N-terminal" evidence="13">
        <location>
            <begin position="91"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="12">
        <location>
            <begin position="197"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013926304.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="51400" checksum="1391167D8E7A062F" modified="2020-10-07" version="1">MMMRKKRKREGSPCSGSCPSPATPPESQRAAWTGMGTAAPFCAIASAAAEVEEQQRRPGRQSRPPPSAALAVLLSLGLLLHAGDCQLQTPCRIQKCTTDFVSLTSHLNSAIDSFDVEFCKALRAYSACTQRNSKVCRGNLVYHSAVLGIGDLMSQRNCSKDGPTSSTNPEVVHDPCNYSGRQATRDYHGGEQIPPTYFFCGLFGDPHLRTFKDHFQTCKVEGAWPLIDNNYLSVQVTNVPVVPGSSATATNKITIIFKSYQDCTDQKVYQAVTDDLPAAFVDGTTSGGDGDPKSLHIVERVSGKYVEMHARYIGTTVFVRQLGRYLTLSIRMPEELALAYEESQDLQLCVNGCPSSERIDDSGHLPLPVKGQLLPSGASSHTRSIYTLESATTKCHEKMLLKDIYFHSCVFDLLTTGDANFTAAAHSALQDVEALHPRKERWHIFPNNEGSVQRGEFGLGLVGFLLLVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPF9</accession>
    <name>A0A6I9YPF9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lipoyl synthase, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106552597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926388.1">
        <property type="nucleotide sequence ID" value="XM_014070913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPF9"/>
    <dbReference type="GeneID" id="106552597"/>
    <dbReference type="KEGG" id="tsr:106552597"/>
    <dbReference type="OrthoDB" id="3231at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="132"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013926388.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="23664" checksum="28232F5A279E5D9E" modified="2020-10-07" version="1">MWLLGSGLPCAALAPRSLTRLLSEGKPMFGNQRRTSSSNSLPEAQMGHFQEGPDLKDFISGELSDKAKWAEYKGNLKRQKGERLRLPPWLKTEIPVGKNYNKLKNTLRHLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTSRNPPPLDPEEPYNTAKAIAEWGLDYVVLTSVDRDDLSDGGAEHFAKTVSHLKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPX1</accession>
    <name>A0A6I9YPX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calglandulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106552553</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the cellular control mechanism of the secretion of toxins from the gland into the venom.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926337.1">
        <property type="nucleotide sequence ID" value="XM_014070862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPX1"/>
    <dbReference type="GeneID" id="106552553"/>
    <dbReference type="KEGG" id="tsr:106552553"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926337.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="18749" checksum="F82EE8BF12423C76" modified="2020-10-07" version="1">MAHRGKGTAASARKKLGPKLELSAAHKEQMREAFDLLDADGTGTIDVRDLKVSIRALGFDPTKEELRRVVAEVDKEGAGKIGFDAFYSVMAQKMSEIDSKEDILKAFKLFVDKDSGRIAFKNLKQIAAEIGEKLTDEELQEMINEADLNGDGEVTEQEFLKVMKRHDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ57</accession>
    <name>A0A6I9YQ57_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alpha-2-macroglobulin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106552808</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926648.1">
        <property type="nucleotide sequence ID" value="XM_014071173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ57"/>
    <dbReference type="GeneID" id="106552808"/>
    <dbReference type="KEGG" id="tsr:106552808"/>
    <dbReference type="OrthoDB" id="9998011at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF165">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="4">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="5">
        <location>
            <begin position="236"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="6">
        <location>
            <begin position="587"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926648.1"/>
        </source>
    </evidence>
    <sequence length="644" mass="71936" checksum="6E22D5D32D60E878" modified="2020-10-07" version="1">MSRGDIVKSGTHPLKEKQSKGIFNLDLLVDIKTAPLARLLVYTLLDNGELVAHSANFPVEPCFANKVDLRFAEREGLPGSQINLHLAAAQNSLCAIHAVDESVFLLKPEAELSAQTVYDLLPLTDLRGYYYKSENLEDPDTNPCVPPKKIIVDGIRYETDTYSYGEGDAYTILRDIGFKTFTSAQIYKPNICSRPERHYPMAYMERGPANARLGGMSMDNSAEPVETTIRRFFPETWIWSLKSLGSESELTLPVTIPDTITDWRAGAFCLSPAVGFGLAQTIFLTAIQPFFVEVTQPYSVIRGEDYVMKATIYNYLKRSIQVGVSLLLSPDYIATPLDKEQGPHCLPVDGRKTFSWKVTPKTIGVQRQLNYKHADGSYSTFGERHNEPGNHSVVRNALFCLETVSQQKDIPIYTQALLAYAFALAHKDDKVTALLQSLQQKAVKGDDGSIHWERIVKPEVKKSLYYEPQALSAEVEMTSYVLLTHLTMLRRAASVSQDDLAIPTKIVTWMTKQQNPSGGFASTQKDDPLTRIFPPQTTLRYNVLPHPGDDPFALTVTTNPETCVGVKAHREFDIAINVSYVGKRPSTNMVILNIKMVSGFAPNRPSVKELEKQALIKRVDMTPREVVVYFEKVSAEATARERVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR58</accession>
    <name>A0A6I9YR58_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">N-alpha-acetyltransferase 15, NatA auxiliary subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NAA15</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926120.1">
        <property type="nucleotide sequence ID" value="XM_014070645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR58"/>
    <dbReference type="GeneID" id="106552386"/>
    <dbReference type="KEGG" id="tsr:106552386"/>
    <dbReference type="CTD" id="80155"/>
    <dbReference type="OrthoDB" id="10263032at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1010:FF:000001">
        <property type="entry name" value="N-alpha-acetyltransferase 15, NatA auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000001">
        <property type="entry name" value="N-alpha-acetyltransferase 15, NatA auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF6">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 15, NATA AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="574"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="606"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013926120.1"/>
        </source>
    </evidence>
    <sequence length="865" mass="101269" checksum="D83C85231037D2A4" modified="2020-10-07" version="1">MPSVSLPPKENALFKRILRCYEHKQYRNGLKFCKQILSNPKFAEHGETLAMKGLTLNCLGKKEEAYELVRRGLRNDLKSHVCWHVYGLLQRSDKKYDEAIKCYRNALKWDKDNLQILRDLSLLQIQMRDLEGYRETRYQLLQLRPAQRASWIGYAIAYHLLEDYEMAAKILEEFRKTQQTSPDKVDYEYSELLLYQNQVLREAGLYKEALDHLGTYEKQICDKLAVEETKGELLLQLCRLEEAAEIYRGLQERNPENWAYYKGLEKALKPANMLERFKIYEEAWTKYPRGLVPRRLPLNFLSGDKFKECLDKFLRMNFSKGCPPVFNTLRSLYKDKEKVAIIEELVVGYETSLKGFRLFNPNDDGKEEPPTTLLWVQYYLAQHYDKIGQPSIALDYINAAIESTPTLIELFLVKAKIYKHAGNIKEAARWMDEAQALDTADRFINSKCAKYMLKASLIKEAEEMCSKFTREGTSAVENLNEMQCMWFQTEYAQAYKTMNKFGEALKKCHEIERHFVEITDDQFDFHTYCMRKITLRSYVDLLKLEDVLRQHPFYFKAARIAIEIYLKLHDNPLTDENKEHETDTANMSDKELKKLRNKQRRAQKKAQLEEEKKNAEKEKQQRNQKKKKDDDDEEIGGPKEELIPEKLAKVEAPLEEAIKFLTPLKNLVKNKIETHLFAFEIYFRKEKFLLMLQSVKRAFAIDPNHPWLHECMIHLFSSVSESKDLQETVRTVLNQEMNRLFGATNPKNFNETFLKQHYNSLPHRLSAAKMVYYLDSTSQKRSVELATSLDESLANRNLQTCMEVLEALNDGSLGDCKEAAEMYRANCHKLFPYALAFMPPGYEEDMKITVNGDISAETEELANEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRE5</accession>
    <name>A0A6I9YRE5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[G-protein-coupled receptor] kinase</fullName>
            <ecNumber evidence="3">2.7.11.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.16" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013927118.1">
        <property type="nucleotide sequence ID" value="XM_014071643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRE5"/>
    <dbReference type="GeneID" id="106553195"/>
    <dbReference type="KEGG" id="tsr:106553195"/>
    <dbReference type="OrthoDB" id="9048001at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000017">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF27">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="10">
        <location>
            <begin position="59"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927118.1"/>
        </source>
    </evidence>
    <sequence length="178" mass="21003" checksum="97F88A78492CE024" modified="2020-10-07" version="1">MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIERDYYSLCDKQPIGRYLFRQFCETRLELECCIKFLDSVVEYELLPDEKLGEKGKEIVMKYLIPGSPVYVAEASQDLVQQIKEKLENSPCKDLFLPCVKSVHEYLSGEPFQEYSDSMYFDRFLQWKWLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRF4</accession>
    <name>A0A6I9YRF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gonadotropin-releasing hormone receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Type II GnRH receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106552698</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926514.1">
        <property type="nucleotide sequence ID" value="XM_014071039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRF4"/>
    <dbReference type="GeneID" id="106552698"/>
    <dbReference type="KEGG" id="tsr:106552698"/>
    <dbReference type="OrthoDB" id="6022667at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000199">
        <property type="entry name" value="Gonadotropin-releasing hormone II receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001658">
        <property type="entry name" value="GphnRH_fam_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF22">
        <property type="entry name" value="GONADOTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00529">
        <property type="entry name" value="GNADOTRPHINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013926514.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="41820" checksum="07075A6887F46C89" modified="2020-10-07" version="1">MDGTFSPSEGPRSSSQLPTFSTAAKIRVALTVLLFLCSTSINLSMLRSVTKTFQRKPHLRILLMNLAAANLLVTFVVMPLDAVWNVTVQWYAGDLACRTLMFLKLVAMYACSFVTVAISLDRWAAILHPLRVSRAKRKNKAMLCVAWTLSFLLAVPQMFVFHTVSRSQPTHFVQCATVGSFGAHWQETLYNMFTFSWLFLVPLLVMVLCYSRILVEISRMMSQARAPPKSREVRLRCSRDNIPQIRIRALKMSAITVLTFVACWTPYYLLGLWYWFSPEMLTREQVPPSLGHIFFLFGLLSTCLDPLVYCFRPSRKLAKAPSLRMASGRASTMSNGPRAEMGVHWACKPGSPRSPKVAWTRKEKVAESCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRG4</accession>
    <name>A0A6I9YRG4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein dpy-30 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Dpy-30-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">DPY30</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927138.1">
        <property type="nucleotide sequence ID" value="XM_014071663.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013927148.1">
        <property type="nucleotide sequence ID" value="XM_014071673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRG4"/>
    <dbReference type="GeneID" id="106553207"/>
    <dbReference type="KEGG" id="tsr:106553207"/>
    <dbReference type="CTD" id="84661"/>
    <dbReference type="OrthoDB" id="417678at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000003">
        <property type="entry name" value="protein dpy-30 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037856">
        <property type="entry name" value="Sdc1/DPY30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23356:SF16">
        <property type="entry name" value="DPY30 DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23356">
        <property type="entry name" value="DPY30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013927148.1"/>
        </source>
    </evidence>
    <sequence length="99" mass="11217" checksum="E65B057BB1EA974C" modified="2020-10-07" version="1">MDTEQIIEGQPQVPENPHAEYGLTENVERIVENEKTSAEKTSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPINPIEFLAAYLLKNKAQFEDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSW5</accession>
    <name>A0A6I9YSW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Voltage-dependent R-type calcium channel subunit alpha-1E-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927392.1">
        <property type="nucleotide sequence ID" value="XM_014071917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSW5"/>
    <dbReference type="GeneID" id="106553425"/>
    <dbReference type="KEGG" id="tsr:106553425"/>
    <dbReference type="OrthoDB" id="9907511at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927392.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="34685" checksum="B3DD08EE23DDAD0F" modified="2020-10-07" version="1">MPRLTVDPQVVTDPGSMRRSFSTIRDKRTNSSWLDEFSMERSSENTYKSRRRSYHSALHLSSRRLNIDSGHRTDTHRSGGRERGRSKERRHLLSPDISRCNSEERNPQADIDSPERPHSRSPSEGRSQTPNRQGTGSLSESSIPSLSDTSTPRRSRRQLPPVPPKPRPLISYTAMMQHCGDTSPPPDDSEGGSPLLSGTLETNNTCLTESSSSLQGKQSLLSTPQRYISEPYLMLQDDSHASDCGEEETLTFEAAVATSLGRSNTIASGPRSRHGWQMPNGHYRRRRRGASQSMYGAGIEVLSDTEEDDKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTU7</accession>
    <name>A0A6I9YTU7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Lethal(3)malignant brain tumor-like protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106553699</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927732.1">
        <property type="nucleotide sequence ID" value="XM_014072257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTU7"/>
    <dbReference type="GeneID" id="106553699"/>
    <dbReference type="KEGG" id="tsr:106553699"/>
    <dbReference type="OrthoDB" id="8188861at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20135">
        <property type="entry name" value="MBT_L3MBTL3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20133">
        <property type="entry name" value="MBT_L3MBTL4_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20139">
        <property type="entry name" value="MBT_L3MBTL4_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09582">
        <property type="entry name" value="SAM_Scm-like-3MBT3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000007">
        <property type="entry name" value="Lethal(3)malignant brain tumor-like protein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004092">
        <property type="entry name" value="Mbt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF130">
        <property type="entry name" value="SAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02820">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00561">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51079">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="MBT" evidence="10">
        <location>
            <begin position="90"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="10">
        <location>
            <begin position="198"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="10">
        <location>
            <begin position="306"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="610"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="454"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="487"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927732.1"/>
        </source>
    </evidence>
    <sequence length="677" mass="76045" checksum="2B8919EC65542021" modified="2020-10-07" version="1">METFENSHQESKDRLSKDNTTNCGKQTNNSQTRETDRPSKRLRRKRKLLLDSEEEEENGDEDEEKNKLNPKNRKNAKPMKQAAPAKKNVWNWMTYLEEERMPAAPLKLFKESQSFPQNKNGFKVGMKLEGLDPEHPSLFCVLSVAEVQGYRLRLHFDGYSEFYDFWVNADSTDIHPVGWCEKTGHKVLPPKGYKDGEFNWASYLKTCKSQAAPKNLFKILSTPVTPSGFRVGMKLEAVDKKNPSLMCVATIADMLDNRLLVHFDNWGESYDYWCDASSPYIRPVGYCQETGTPLTTPAEYKDFKSFSWEKYLEETGSQAAPARAFKLRPAHGFQPNMKLEAVDRRNPMLIRVATITEKDDHRVKVHFDGWDHAFDFWVDADSPDIHPMGWCAKTGHALQLPPGTSNAASMPAGQVCPTPGCQGLGHVRGPRYGTHYTLIGCPYSETSRTRDGLLQDRLGGEKPSPGSGAPKPKKSDISAQLPDLQGDSPQSRKSSTQEGEGSGRSSVHSFSEQSETSQEKDWAEGERPTITKIKAKGIGCSHSYIKLQLVKQEADGERDTAFALQQTLHQSVFMPSLSANPTHRLHLCWEQHCKLLPEVSGLTAKNVAKWSVEEVAGFVQRLPGCKEQAALFRQEQIDGEAFLLLNQSDIVKILSIKLGPALKIYNAILMFKTAEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTW7</accession>
    <name>A0A6I9YTW7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L18a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RPL18A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927486.1">
        <property type="nucleotide sequence ID" value="XM_014072011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTW7"/>
    <dbReference type="GeneID" id="106553498"/>
    <dbReference type="KEGG" id="tsr:106553498"/>
    <dbReference type="CTD" id="6142"/>
    <dbReference type="OrthoDB" id="1294322at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002190">
        <property type="entry name" value="Ribosomal_L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="5">
        <location>
            <begin position="7"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013927486.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="20708" checksum="77F6E0C950A448F6" modified="2020-10-07" version="1">MKASGTLREYKVIGRCLPTPKCPTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUB5</accession>
    <name>A0A6I9YUB5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bcl-2-related protein A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">BCL2A1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927897.1">
        <property type="nucleotide sequence ID" value="XM_014072422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUB5"/>
    <dbReference type="GeneID" id="106553846"/>
    <dbReference type="KEGG" id="tsr:106553846"/>
    <dbReference type="CTD" id="597"/>
    <dbReference type="OrthoDB" id="8856583at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020717">
        <property type="entry name" value="Bcl2_BH1_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020726">
        <property type="entry name" value="Bcl2_BH2_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013282">
        <property type="entry name" value="Bcl2A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF10">
        <property type="entry name" value="BCL-2-RELATED PROTEIN A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01867">
        <property type="entry name" value="BCL2RLATEDA1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01080">
        <property type="entry name" value="BH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01258">
        <property type="entry name" value="BH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="3">
        <location>
            <begin position="37"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013927897.1"/>
        </source>
    </evidence>
    <sequence length="173" mass="19712" checksum="EBB7C9468660C419" modified="2020-10-07" version="1">MESCNFLYVNTLVQDYLKYICQEAQLSAAPNRVAEVLRKAGSSAQREVEDKLRPYVDSLEIHSVEGASHVFSQVMENEFVDGEINWGRILTIFLFGGILAKKLQGPLAKENLKQISYFITDYIVSTKGKWINENGGWEKGFITKFEDRSSWVSLSTLKTKILAVFSIFNQYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUC0</accession>
    <name>A0A6I9YUC0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NHS-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927902.1">
        <property type="nucleotide sequence ID" value="XM_014072427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUC0"/>
    <dbReference type="GeneID" id="106553848"/>
    <dbReference type="KEGG" id="tsr:106553848"/>
    <dbReference type="OrthoDB" id="8965057at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024845">
        <property type="entry name" value="NHS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23039">
        <property type="entry name" value="NANCE-HORAN SYNDROME PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23039:SF3">
        <property type="entry name" value="NHS-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15273">
        <property type="entry name" value="NHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927902.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="28290" checksum="619CD7594356946D" modified="2020-10-07" version="1">MAPAVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEELHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQDPTFSSSSNTLYESYQDDYEEIEQKSSLLDCLSQSCLSACCCLMSWTIKCSTPSPEEEKLIPVKRPKSPTPDELSDINTQTNWTKSLPLPTPEEKMRQQAQAVPADVIPINVTGENFDRQASIRRSLIYTDTVVRRPKKMKRRKTITGVPDNIQRELGMAHQNCIPFQLIAYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUC4</accession>
    <name>A0A6I9YUC4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Disintegrin and metalloproteinase domain-containing protein 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106553854</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927907.1">
        <property type="nucleotide sequence ID" value="XM_014072432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUC4"/>
    <dbReference type="GeneID" id="106553854"/>
    <dbReference type="KEGG" id="tsr:106553854"/>
    <dbReference type="OrthoDB" id="9017896at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF112">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0800">Toxin</keyword>
    <feature type="domain" description="ADAM cysteine-rich" evidence="6">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013927907.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="32697" checksum="B0335E3A49E51465" modified="2020-10-07" version="1">MCQTHEQQCITLWGPGAKPAPEICFQRVNSAGDPYGNCGKDSKSTFAKCEPRDSKCGKIQCQGGASRPVIGTNAVSIETNIPLPGGGKILCRGTHVYLGDDMPDPGLVLAGTKCEDGKICLNRRCQNTSVLGVHQCATKCHGRGEIHMLILLSVSQTRPSSSSESNHVHTATSLSKKLDKQPQNSTTPRRNNIQQLLQYQKRDIGYFGKAQDVSKWSPPERIQPHLSELSSQQSVFGKGLPTKPPLKFDLESCKPSPPQKPLPANPQKNKAVQIKPSSRTMFELPKPMPQIAPVRMWNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV62</accession>
    <name>A0A6I9YV62_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">U6 snRNA-associated Sm-like protein LSm7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LSM7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome. Interacts with TACC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927894.1">
        <property type="nucleotide sequence ID" value="XM_014072419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV62"/>
    <dbReference type="GeneID" id="106553843"/>
    <dbReference type="KEGG" id="tsr:106553843"/>
    <dbReference type="CTD" id="51690"/>
    <dbReference type="OrthoDB" id="2146at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01729">
        <property type="entry name" value="LSm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000025">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017132">
        <property type="entry name" value="Lsm7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="10"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927894.1"/>
        </source>
    </evidence>
    <sequence length="103" mass="11574" checksum="B289DC1D31F5DB4A" modified="2020-10-07" version="1">MADKEKKKKESILDLSKYIDKTIRVKFQGGREASGVLKGFDPLLNLVLDGTIEYMRDPDDQYKLTEDTRQLGLVVCRGTSVVLICPQDGMEAIPNPFIQQQDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVY2</accession>
    <name>A0A6I9YVY2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Olfactory receptor 5V1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106554604</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928788.1">
        <property type="nucleotide sequence ID" value="XM_014073313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVY2"/>
    <dbReference type="GeneID" id="106554604"/>
    <dbReference type="KEGG" id="tsr:106554604"/>
    <dbReference type="OrthoDB" id="6145535at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="28"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928788.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="24562" checksum="9185F58E23941ABB" modified="2020-10-07" version="1">MILLHPQPVFAGGINSKEVPEKSTLPPRISAVCPAAICQPLHYAHLMRRKVCIQLSVAIWLWGFLHSTIHVALASRLDFCGNNNIPHIFCDVPPLLKIACSDTWANELANHITSLFVGLSPFLFIILSYVCILTSILRIRSNTGRRKAFSTCASHISVVTLFVGNGSMNYNQPSAGYSLEVDALIATMFCIVTPMLNPLIYSLHNREVKGALRKIFNCMKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZ59</accession>
    <name>A0A6I9YZ59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Receptor-type tyrosine-protein phosphatase zeta</fullName>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">PTPRZ1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013929334.1">
        <property type="nucleotide sequence ID" value="XM_014073859.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555090"/>
    <dbReference type="KEGG" id="tsr:106555090"/>
    <dbReference type="CTD" id="5803"/>
    <dbReference type="OrthoDB" id="6022401at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03122">
        <property type="entry name" value="alpha_CARP_receptor_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000016">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000313">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000004">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000013">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041887">
        <property type="entry name" value="Alpha_CARP_receptor-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF461">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="27" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027106525" description="Receptor-type tyrosine-protein phosphatase zeta" evidence="21">
        <location>
            <begin position="26"/>
            <end position="2283"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="25">
        <location>
            <begin position="37"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="315"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="22">
        <location>
            <begin position="1692"/>
            <end position="1960"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="23">
        <location>
            <begin position="1877"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="22">
        <location>
            <begin position="1991"/>
            <end position="2250"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="23">
        <location>
            <begin position="2167"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="531"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1357"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1456"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1519"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2259"/>
            <end position="2283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1364"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1375"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="2259"/>
            <end position="2268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00059596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00068084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013929334.1"/>
        </source>
    </evidence>
    <sequence length="2283" mass="253941" checksum="360960F06ACE47E3" modified="2020-10-07" version="1">MRLLRHFLLLSVQLFCLCPIELILGYFRPPRKFAEAVDWSYSGALNQTKWGKKYPACNGAKQSPINIDEDLTQVNVNLKQLKFKGWEEETSEDTLIYNTGKTVEIKLKNEYFVIGGGSEAIFKASKITFHWGKCNISHDGSEHSIDGQKYPLEMQIYCYDSNEFSDIDKAIEGNGKLRTLSVLFEVGLEDNMDYNSIIEGVASVSRFGKKNELEPFILLNLLPNSTDKYYTYNGSLSTPPCSETVEWIVFKDTVNISENQLSVFCDVLTMQQSGYVMLMDYLQNNFRDQQHKFSGQVFSSYTGQEEIHEAVCSLEPENVQADPKNYTSLLVTWERPRVVYDTMIEKFAVFYQQLEGEDQTKHEFLTDGYQDLGAILNNLLPNTSYILQIVAICANGLYGKYSDQVIVDMPLDDPEIELFPELTETEDYEEEDEMEDERTGLDEDATVIASRDSATNQIKKSKFQFTTSSYSQERMETKFSEDGIPRYFTEEKYLHDRSKATFDQTFPPFTEGLKEEENNPMEFQAATAIVPPASSSTKGNEEEYAHTFSGNETTRESAADSNEDDIEVSAFKPDLETDDSWGSIPITSATRLVTLETSQENTFSSGSPDTTTQDDLSQNYVKILSDDMLPSKAEESAPKISAINENEWYPNSTDSTAVALDSKDLSNVNSPIPTTALYPNDPEGPQDTSVAVTVLLHSSTFALSSSELSSYSVSSNSEERGSASSGSEVLSQTTQAVYNGEIPLQPSYSSEVFPLVTPLLSDTQLLNISPEASSSDVTLHATPVFPSVGMSFESTLSSYDDVPLLPFSSASSSSKMFHHLYMVSQTFPQVAPAIENDKGSLHASLTLEEGGTLTKSWLTQFSDVTSYQMVHATSETLGFGHDRTHLLSQVEPPSNDFNGHEVSTMSESVYALSGNMGSPQTVAASYHTSEFVHESVGTFHHGPLFSIYSNMPVPKPSSAVSQADTFLQPPCFLSSETDCSEAHSGSESLLPDTDIWSVLKASSHVSSSEVMCSTPVFTGEVEMTPQNGVMHGYDKGLQISASHTGNEMTEPPPSISNYEIPKQDTRPQESLITVSSAKGILPGSIAFPATKVFDYYVSKRLENVPIRSLHVATPVFDDTLLKPMLSANSQQASSTPASSKTLPSPSQQYFYWASATLNNISLLQTSFQSSNDDTVRKTSVPDDPVLVENVTNGHSSNFVFNQILPQIATNAAEYKKVLHSTSKPSAVNISHTVGKAFSSPESLTFPHISDEYISPYLHKSEDIQQVSPSLYNNGVPYPPTNFENKNAFPLNAVNTVTIPIGPTEMLPNAATFAHNLPNDWPKAESLTLSSSAKIVDVTFSMPEVIADSIQHASINAPDTHRSLTADSSSSEGSKSLNPLLIPTQLLPTTSSDREPWSSLVDHEYDEYDSGLPVSGCHTCISNRETREEVDEPATDLNNANSHQNIIRKLHFERPQKKEAKKILSSLSDHTVTEETDQLKQASVPAMAPNRKMKKHGDQALLDNSAQTLSNPLPRTLESKSWSVFTSDEESGSGPGTSDSINEEMSTDFSFFGVNEHDLEGVVGTGNSELTPGLQESSIEFISRAVSTVFNISEAEASNSSHQLIIGLPEQKTVIPLVIVSTLTFTCLVILVAILIYWRKCFQTAHFYLEDNTSPRVISIPPAPVFPVSDDVGAIPTKHFPKHVADLHASNGFPEEFEEIQSCTVDLGITSDSSNHPDNKNKNRYINIVAYDHSRVKLTQLSDKDGKVTDYINANYVDGYNRPKAYIAAQGPLKSTAEDFWRMIWEHNVEVIVMITNLVEKGRRKCDQYWPADGSEEYGNFLVTQKSIHVLAYYTVRNFTIRNTKVKKGSQKGRSSGRLVSQYHYTQWPDMGVPEYTLPVLAFVRKAAYAKRHAVGPVVVHCSAGVGRTGTYIVLDSMLQQIQHEGTVNIFGFLKHIRTQRNYLVQTEEQYIFIHDALVEAILSKETEVPENHIHAYVNSLLIPGPMGKTRLEKQFKLLNQSNIQQCDYSTALKQYNREKNRTSSIIPVERSRVGISSLSGEGTDYINASYIMGYYQSNEFIITQHPLLHTIKDFWKMIWDHNAQIIVMLPDSQNMAEDEFVYWPNKEDPINCESFKVSLISEEHKCLSNEEKLIIQDFILEATQDDYVLEVRHFQCPRWPNPDSPISKTFELISIIKEETSNRDGPMIVHDEYGGVTAGTFCALTTLMHQLENENCVDVYHVAKMVNLMRPGVFTDTDHYQFLYKTVLSLVSSRQEENPSISIDSNGSVLPDGSAAESLESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1Q3</accession>
    <name>A0A6I9Z1Q3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sortilin-related receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Low-density lipoprotein receptor relative with 11 ligand-binding repeats</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Sorting protein-related receptor containing LDLR class A repeats</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">SORL1</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endosome</location>
            <location evidence="7">Multivesicular body membrane</location>
            <topology evidence="7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
            <location evidence="5">trans-Golgi network membrane</location>
            <topology evidence="5">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Recycling endosome membrane</location>
            <topology evidence="6">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the VPS10-related sortilin family. SORL1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929765.1">
        <property type="nucleotide sequence ID" value="XM_014074290.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555440"/>
    <dbReference type="KEGG" id="tsr:106555440"/>
    <dbReference type="CTD" id="6653"/>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000011">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000006">
        <property type="entry name" value="Putative low-density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000416">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000461">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001616">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000027">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000030">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000036">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000039">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000060">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.80:FF:000002">
        <property type="entry name" value="Sortilin-related receptor isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000021">
        <property type="entry name" value="Sortilin-related receptor isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000002">
        <property type="entry name" value="Sortilin-related receptor isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000033">
        <property type="entry name" value="Sortilin-related receptor isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057841">
        <property type="entry name" value="FN3_SORL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
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        <property type="entry name" value="LDLRECEPTOR"/>
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    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="11" id="KW-0813">Transport</keyword>
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            <end position="294"/>
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</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1Z7</accession>
    <name>A0A6I9Z1Z7_9SAUR</name>
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        <submittedName>
            <fullName evidence="7">Iron-responsive element-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106556610</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014075598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1Z7"/>
    <dbReference type="GeneID" id="106556610"/>
    <dbReference type="KEGG" id="tsr:106556610"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Iron-responsive element binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Iron-responsive element binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
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        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
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        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
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        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
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        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="5">
        <location>
            <begin position="75"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="5">
        <location>
            <begin position="214"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="139"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013931073.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="44730" checksum="85D0145217AB303C" modified="2020-10-07" version="1">MEAARAGHLFQHLIETLSDDADKKFYNIAKLGGTKYDLLPYTIRVMLETAVRNWDGFLVKEGDVMNILDWPAKQNNVEVPFCPARVLLQDFTGIPAMVDFAAMREAVKNLGGDPTRVHPVCPTDLTVDHSLQIDFNKCAIQNTPNPGGGDPPRPPPSKLSPLKAPRKLPCRGQSSCKGPCDGESKRNPGQLSVQIENTPLLCPFHLQPVPEPETVFKNQEIEFGRNKERLQFFKWGSKVFRNVSVIPPGTGMVHQMNLEHLSRVVFDVKNFLYPDSVIGTDSHTTMVNGLGILGWGMGGIETEAVMLGLPITLTLPEVVGCELTGSASSLATSIDVVLSITKQLRQAGVSGKFVEFFGPGVSQLSVGDRTTIANMCPEYGAILSFFPVDDVTLKHLKHTGKIDSVGGDLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2N5</accession>
    <name>A0A6I9Z2N5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Neuron-specific calcium-binding protein hippocalcin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">HPCA</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Calcium-binding protein that may play a role in the regulation of voltage-dependent calcium channels. May also play a role in cyclic-nucleotide-mediated signaling through the regulation of adenylate and guanylate cyclases.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomer; oligomerization is calcium-dependent. May interact with the voltage-dependent P/Q- and N-type calcium channels CACNA1A and CACNA1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931327.1">
        <property type="nucleotide sequence ID" value="XM_014075852.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931328.1">
        <property type="nucleotide sequence ID" value="XM_014075853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2N5"/>
    <dbReference type="GeneID" id="106556838"/>
    <dbReference type="KEGG" id="tsr:106556838"/>
    <dbReference type="CTD" id="3208"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF57">
        <property type="entry name" value="NEURON-SPECIFIC CALCIUM-BINDING PROTEIN HIPPOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013931327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013931328.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="22327" checksum="5E731EBA0DAB0C55" modified="2020-10-07" version="1">MGKQNSKLRPEMLQDLRENTEFSDLELQEWYKGFLKDCPTGILNVEEFKKIYANFFPYGDASKFAEHVFRTFDTNGDGTIDFREFIIALSVTSRGKLEQKLMWAFSMYDLDGNGYISREEMLEIVQAIYKMVSSVMKMPDDESTPEKRTEKIFRQMDTNNDGKLSLEEFIKGAKSDPSIVRLLQCDPSSASQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2W4</accession>
    <name>A0A6I9Z2W4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Secreted frizzled-related protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106555930</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secreted frizzled-related protein (sFRP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930346.1">
        <property type="nucleotide sequence ID" value="XM_014074871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2W4"/>
    <dbReference type="GeneID" id="106555930"/>
    <dbReference type="KEGG" id="tsr:106555930"/>
    <dbReference type="OrthoDB" id="5985572at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000001">
        <property type="entry name" value="secreted frizzled-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF149">
        <property type="entry name" value="SECRETED FRIZZLED-RELATED PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="FZ" evidence="10">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013930346.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="25136" checksum="D715256C97BBB7FD" modified="2020-10-07" version="1">MSLCHGVGYSEMRLPNLLGHDTMKEALQQAASWVPLLTKQCHRETKKFLCSLFAPVCISQVEEPIFPCRSLCEAVRDSCLPVMAAFGFPWPEMLNCSRFPGGNELCIPPVGPEDQEQPPREALKMTIKSFSGVGGDLKVIPELRGRTLYKQASWSEEERKKPVLWLPEGEACSCEELAEGPGTVVLAMGHRLSNRLVLSWVRRWKHGEKELKRFSRAVRKLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3C1</accession>
    <name>A0A6I9Z3C1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106556699</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013931147.1">
        <property type="nucleotide sequence ID" value="XM_014075672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3C1"/>
    <dbReference type="GeneID" id="106556699"/>
    <dbReference type="KEGG" id="tsr:106556699"/>
    <dbReference type="OrthoDB" id="10070999at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000012">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF140">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED SERINE_THREONINE-PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931147.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="21206" checksum="9A09E856192284C4" modified="2020-10-07" version="1">MEYVEGGDCATLLKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMNMTTNLYEGHIEKDTREFVDKQVCGTPEYIAPEVILIQGYGKPVDWWAMGIILYEFLIGCVPFFGDTPEELFGQVISDEIIWPEGDEALPADAQDLITQLLRHCPLERLGTGGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z498</accession>
    <name>A0A6I9Z498_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox protein Hox-D1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106556588</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931049.1">
        <property type="nucleotide sequence ID" value="XM_014075574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z498"/>
    <dbReference type="GeneID" id="106556588"/>
    <dbReference type="KEGG" id="tsr:106556588"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046327">
        <property type="entry name" value="HXA1/B1/D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946">
        <property type="entry name" value="HOMEOBOX PROTEIN ROUGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="216"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="218"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931049.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="29324" checksum="FDC3308804F63890" modified="2020-10-07" version="1">MNSFLEYLSRGGDSLSLAGKFSSAEPGSVALRSPCALSAGAVDGVYPGPPPPPPVGSLPDFSKRLAPPRQAPRLQPQQLAPCALPSHLFPSAEFNLLRPEAPYAGPEESAPLHYATSIFPGGGGGGGGGSGGAYLQMDYTALAETFQPCPKDALLRPPPPPDFCPKAGGQASVSTFDWMKVKRNAPPKSKCRGLVNACPVGKPTACGSSSLPSVVRTNFSTKQLTELEKEFHFNKYLTRARRVEIAKSLGLNDTQLISIVWHSSSPAPSVLLTVLTWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4M4</accession>
    <name>A0A6I9Z4M4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Fibrillin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106557357</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibrillin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932053.1">
        <property type="nucleotide sequence ID" value="XM_014076578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4M4"/>
    <dbReference type="GeneID" id="106557357"/>
    <dbReference type="KEGG" id="tsr:106557357"/>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000049">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000058">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000071">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000086">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000141">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000007">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000009">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000003">
        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000008">
        <property type="entry name" value="Signal peptide, CUB domain, EGF-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF4">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
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        <property type="match status" value="5"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="19"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="65"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="111"/>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
            <begin position="197"/>
            <end position="248"/>
        </location>
    </feature>
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        <location>
            <begin position="269"/>
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        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="311"/>
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        </location>
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    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="353"/>
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        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="395"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="478"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="519"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="584"/>
            <end position="625"/>
        </location>
    </feature>
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        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013932053.1"/>
        </source>
    </evidence>
    <sequence length="626" mass="67229" checksum="7A885E7B55001187" modified="2020-10-07" version="1">MESEKNEKEREKEKVGGGDIDECQTPGICMNGWCINTEGSFRCECLGGLAIGVDGRGCVDTHVRSTCYGGIKKGTCARPFPGAVTKSECCCANPDHGFGEPCHPCPAKNSDVDECAINRLLCDNGLCRNTPGSYSCSCPKGFVFQTETDTCEDINECTSNPCVNGLCRNNAGSFACECSPGSKLDPSGTICVDVRLEQCYMKWAEDECTVALPGKYRIDLCCCSVGAAWGKDCEECPKPGSAEFKAICPRGPGFANRGDLLSGRPFYKDVNECKMFSALCTHGTCRNTIGSFRCLCGNGFALDAEERNCTDIDECERDPLLCRGGVCVNTEGSFECLCPPGHELTAEGNVCVDIDECEDTPDICDGGQCTNIPGEYRCLCFDGFMASLDMKTCIDVNECDLNPNICLHGECENTKGSFICHCQLGYFVKKGTTGCTDIDECTILNGGCDTHCANAEGSYECSCGEGYALMPDKRSCVDLDECATEAHSCNLNANCLNTPGSFRCACQEGFSGDGYSCSDVDECADNVNLCENGQCLNAPGGYRCECEMGFNPTDDSKACQAEYKTLCPGGEGFRPNPITVILEDIDECQELPGLCQGGNCVNTFGSFQCECPPGYYLNEDTRICEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5Q4</accession>
    <name>A0A6I9Z5Q4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ethanolamine kinase</fullName>
            <ecNumber evidence="16">2.7.1.32</ecNumber>
            <ecNumber evidence="13">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CHKB</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>choline + ATP = phosphocholine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12837"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.32"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:12838"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ethanolamine + ATP = phosphoethanolamine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13069"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:58190"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.82"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13070"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.32" evidence="16"/>
    <dbReference type="EC" id="2.7.1.82" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013931524.1">
        <property type="nucleotide sequence ID" value="XM_014076049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5Q4"/>
    <dbReference type="GeneID" id="106556977"/>
    <dbReference type="KEGG" id="tsr:106556977"/>
    <dbReference type="CTD" id="1120"/>
    <dbReference type="OrthoDB" id="3649325at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05156">
        <property type="entry name" value="ChoK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1200.10:FF:000005">
        <property type="entry name" value="Choline kinase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF35">
        <property type="entry name" value="CHOLINE_ETHANOLAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013931524.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="43644" checksum="D18C4D6C58977983" modified="2020-10-07" version="1">MEAPEGDGPDGDRAAPRSPRGSPAVPAATRLRAFLWCREFLAGAWRRLAAPEQLGIVPVSGGLSNLLYRCSLPDHIPSAQEEPRQVLLRVYGVILQGVDSLVLESVMFAILAERALGPRLYGIFPQGRLEEYIPSRRLVTEDLLEPTISGEIAVKMARFHGMVMPFNKEPKWMFGTMERYLKQISELTFSQEAQRHKLNRLKAYNLEAEMHSLRTLLEATPSPVVFCHNDVQEGNILLLARREASSTDRLMLIDFEYSSYNYRGFDLANHFCEWVYSYTHDEWPFYKASPEKYPSRQQQLHFIRCYLQEAGGKADPPSAEEQERLEAKMLLEISRFTLASHFFWGLWSILQAKISTIEFGYLEYAQCRFEAYFQQKARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z619</accession>
    <name>A0A6I9Z619_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Advillin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106557050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931639.1">
        <property type="nucleotide sequence ID" value="XM_014076164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z619"/>
    <dbReference type="GeneID" id="106557050"/>
    <dbReference type="KEGG" id="tsr:106557050"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000027">
        <property type="entry name" value="Villin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF33">
        <property type="entry name" value="ADVILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="4">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="4">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="4">
        <location>
            <begin position="267"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013931639.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="36763" checksum="5AFB73A824C6F267" modified="2020-10-07" version="1">MALTTAFKAVTNMPGLIIWRVEKMDLVLVPPQTHGNFYEGDCYVLLSTRKLGSTLSYDVHYWIGSCSSRDEQGSAAIYTIQLDDYLGGVPVQHREVQGYESELFKGYFKKGVIYKKGGRASGFNHVESNVYNVKRLLHVKGKRNVMAKEVEISWDSFNLGDVFLLDLGKIIVQWNGPESNKQERLKAMLLAQDIRDRERGGRAHIGVIEGDDEEASSDLMKVLESELGERTQEIKPAISDEVVDQQQKASLFLYCVTDSGGKIEITEEARRPLRQELLNHNDCYILDHSGMKIYVWKGKGATKMEKQTAMSKALVCFHFSNFVHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z8E1</accession>
    <name>A0A6I9Z8E1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cardiotrophin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106557397</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-6 superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932106.1">
        <property type="nucleotide sequence ID" value="XM_014076631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z8E1"/>
    <dbReference type="GeneID" id="106557397"/>
    <dbReference type="KEGG" id="tsr:106557397"/>
    <dbReference type="OrthoDB" id="9948731at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010681">
        <property type="entry name" value="PRF/CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21353">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21353:SF8">
        <property type="entry name" value="CARDIOTROPHIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06875">
        <property type="entry name" value="PRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013932106.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18666" checksum="5403671103A0B254" modified="2020-10-07" version="1">MGILKAVVIGLRHQGRPFTDKDFQVQGLSYDGLPVAAISFREWRILDDLDRLDKNYKAYTIILEFLQLVWDDQFELNPTEDGLLNMLKDTRMQVQGLVNNLTIIISALRATPSPVTDPLTLDVVKTNTFEKKVRGYVVCSTYKQWIDRTVRDFNLLVKTYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WVZ1</accession>
    <name>A0A6I9WVZ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RH-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106537482</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907094.1">
        <property type="nucleotide sequence ID" value="XM_014051619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WVZ1"/>
    <dbReference type="GeneID" id="106537482"/>
    <dbReference type="KEGG" id="tsr:106537482"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF43">
        <property type="entry name" value="BLOOD GROUP RH(CE) POLYPEPTIDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="7">
        <location>
            <begin position="44"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013907094.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="38037" checksum="E3399BB7E728F006" modified="2020-10-07" version="1">MFDKLIDTNTIGWHVEAPLHLDHIRNGDCFCCPQIQSHVSMMHAHLFGAYFGLMVSWTLYHSSLSRKAEKERSRPVSDMFAVLGTLFLWIFWPSFNSALIMEEDKKWTAIYNTYFVMATSTIAAFSFSIATSKNGKLSMAHIQNATLAGGVALGFSASNIQQPWIAMVLGLAAGTVSVLGMACLQKRLDPALKIHDTSGVLYTFGLPSLLGGIIHVILILTNHSQNLSVLGYSALIEVGALSLSLCLSLFSGLLTGLTLTCKLWKTPPVTKYFDDQVYWEVGLYLSLDGQLYIKILFLFASWQQGYVELNVLGEGSAALNTQRAPKFQTRSLCSILEPTGNPVPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WY91</accession>
    <name>A0A6I9WY91_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Neuromedin-U receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NMUR2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor for the neuromedin-U and neuromedin-S neuropeptides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907909.1">
        <property type="nucleotide sequence ID" value="XM_014052434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WY91"/>
    <dbReference type="GeneID" id="106538052"/>
    <dbReference type="KEGG" id="tsr:106538052"/>
    <dbReference type="CTD" id="56923"/>
    <dbReference type="OrthoDB" id="5950040at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001607">
        <property type="term" value="F:neuromedin U receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005390">
        <property type="entry name" value="NeuromedU_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005392">
        <property type="entry name" value="NeuromedU_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045561">
        <property type="entry name" value="NMU-R2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF14">
        <property type="entry name" value="NEUROMEDIN-U RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19285">
        <property type="entry name" value="NmU-R2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01565">
        <property type="entry name" value="NEUROMEDINUR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01567">
        <property type="entry name" value="NEUROMEDNU2R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="60"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013907909.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="43629" checksum="B8788966E0AD842A" modified="2020-10-07" version="1">MDCTTNISQYVHRMEESEPLRKYFNSTEDYLLFLYGPKHSHLSLPMTWIYALIFIVGVSGNLLVCLVILKHRNMQTPTNCYLFSLAISDLLVLLFGMPLEVYEMWSNYPFLFGPVGCYFKTAFFETVCFASILIITMVSIERYMAILHPFRARLKNTQQRALRIILTLWLLSIIFSLPNTSIHGIKLQYFPNRTEVPGSAICTVVYSMWIYNWIIQLTSLLFYVLPMSVISILYCLMGLKLRRDRSLEVKEMQMNIKLSSRKSITKMLFVLVIVFGVCWAPFHTDRLFYSFVATWTEPLANIFNLIHVVSGVFFYLSSAVNPIIYHVFSQRFRMAFLSVNFPQCKYWHPRRPTSHPTSQQSVFTLGETQQSRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYZ0</accession>
    <name>A0A6I9WYZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">5'-nucleotidase</fullName>
            <ecNumber evidence="14">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NT5C3B</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>CMP + H2O = cytidine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="3.1.3.91"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(7)-methyl-GMP + H2O = N(7)-methylguanosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37107"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:20794"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58285"/>
            <dbReference type="EC" id="3.1.3.91"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrimidine 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013908174.1">
        <property type="nucleotide sequence ID" value="XM_014052699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYZ0"/>
    <dbReference type="GeneID" id="106538253"/>
    <dbReference type="KEGG" id="tsr:106538253"/>
    <dbReference type="CTD" id="115024"/>
    <dbReference type="OrthoDB" id="10014216at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07504">
        <property type="entry name" value="HAD_5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.340:FF:000001">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000032">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.340">
        <property type="entry name" value="Pyrimidine 5'-nucleotidase (UMPH-1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006434">
        <property type="entry name" value="Pyrimidine_nucleotidase_eu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01544">
        <property type="entry name" value="HAD-SF-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045:SF15">
        <property type="entry name" value="7-METHYLGUANOSINE PHOSPHATE-SPECIFIC 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05822">
        <property type="entry name" value="UMPH-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01128">
        <property type="entry name" value="C1.4:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013908174.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="34291" checksum="00275F84E28C1B32" modified="2020-10-07" version="1">MVPELQKITVRMKNPEHVKNIVSALRKGGAARLQVISDFDMTLTRFGFNGKRCPTSHNIIDNCRVISEEGRKKLKDLLHHYYPIEIDPYRTMEDKLPLMIEWWTKAHNLLSQEKILKNDIAQIVKESDVKLRDGFNIFFDQLHENKVPLFIFSAGVGDVLEEIIHQAGVFHPNINVVSNYMDFDDNGILQGFKEPLIHIYNKNNTVLKNTEYFQQLCTRTNILLLGDSMGDLSMADGVADMENILRIGFLNDRVEERREKYIEAYDIVLEKDETLDVVNGILHFILAQTLYTPLTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZQ5</accession>
    <name>A0A6I9WZQ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PRTFDC1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013908459.1">
        <property type="nucleotide sequence ID" value="XM_014052984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZQ5"/>
    <dbReference type="GeneID" id="106538487"/>
    <dbReference type="KEGG" id="tsr:106538487"/>
    <dbReference type="CTD" id="56952"/>
    <dbReference type="OrthoDB" id="9449045at2759"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="term" value="F:nucleotide binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:IMP salvage"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="term" value="P:purine ribonucleoside salvage"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.2020:FF:000053">
        <property type="entry name" value="Hypoxanthine phosphoribosyltransferase"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HGPRT"/>
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    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
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        <property type="entry name" value="PRTase_dom"/>
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    <dbReference type="NCBIfam" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
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    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013908459.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="18787" checksum="332BB671C36E6FB3" modified="2020-10-07" version="1">MQDIGYNDIVVLCVLKGGYKFCADLVEHIKNLSRNSEKFISMKVDFVRVKSYQNDQSTEDTRIIGGDDFSKLAGKNVLIVEDIIGTGKTMKALLDSIKKYKPSMVKVASLLVKRTVLPDGFRPDYYGFEIPDLFVVGYALDYNEYFRDLNHLCVINNHGKAKYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZW1</accession>
    <name>A0A6I9WZW1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Piezo-type mechanosensitive ion channel component 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PIEZO2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIEZO (TC 1.A.75) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014051562.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tsr:106537433"/>
    <dbReference type="CTD" id="63895"/>
    <dbReference type="OrthoDB" id="303066at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056769">
        <property type="entry name" value="Piezo_TM1-24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056768">
        <property type="entry name" value="THU_Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF6">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24871">
        <property type="entry name" value="Piezo_TM1-24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23188">
        <property type="entry name" value="THU_Piezo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="66"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="122"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="213"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="219"/>
            <end position="240"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="247"/>
            <end position="270"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="320"/>
            <end position="337"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="471"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="528"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="668"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <begin position="992"/>
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        <location>
            <begin position="1044"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1096"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1166"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1188"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1223"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1843"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1882"/>
            <end position="1900"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2106"/>
            <end position="2129"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2149"/>
            <end position="2168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2177"/>
            <end position="2195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2207"/>
            <end position="2225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2246"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2577"/>
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        <location>
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            <end position="1958"/>
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            <begin position="2104"/>
            <end position="2341"/>
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    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="12">
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            <begin position="2378"/>
            <end position="2664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="414"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="605"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1363"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1430"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1468"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1629"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1688"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1760"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2008"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="442"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="628"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
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            <begin position="1468"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="2016"/>
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        <location>
            <position position="1"/>
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    <evidence type="ECO:0000256" key="1">
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF24871"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24874"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013907037.1"/>
        </source>
    </evidence>
    <sequence length="2667" mass="308464" checksum="9A3345D35D1AE0B7" modified="2020-10-07" version="1">LSLSCAFRYNGLSFVYLIYLLLIPLFSEPTKITMQGHTGRLLKSLCFMSLTFLLVHIIFQITIASLEAAKTIQPNYNCLTWEKALRQIGFESVKGADAGNGIRIFVPDILMFVASLVTWLVCRNIVQKPEQENLGEYNAQFENEDMNEIERMEAEDALICEDLDDAECGEEEFEETTKLKILRRIASIASKVKEIIGNLITTAGKVVVTILLGTTGMMLPSLTSAVYFFLFLGLCTWWSCCRAFDPLIFSCLCVLMTIFSGGHLIALYLYQLQFFQEIIPPGDYYARLFGITPVIQTNCSSTWKLITNQKLKWYHHTNPILLLLLYYTLATLIRLWLNEPIVQQMPDDDKDINKEDEIECTPQITADRRRSLWYTSHYTTDERKLLSMTQDEHKPSDVLLVTLNGTPVDYQNIHPLRPIENGPARTDLYSRPQYKWDPSANDLEEEEEEEEGEEEEEEENSEEEKNSKVHAMVSVFHFIMKQSYVCALIAMMAWSITYHSWLTFILLIWSCILWMLRDRRKYAMISSPFMVVYGNLLLTFQYIWSFEMSNSELPQVSGFLERKDPPELGSKILFGLTFWLLLRQHLTERKSLQLNETILSEVKIGEEESEEKDDESQKEEESSKEDSLKEEEDGGELEEKKEEDEEEEVEEEEEEEEDDVMKVLGNMVMAMFVKYWIYVCGGMFFFVSFEGKIVMYKIIYMMLFLFCVVLYQVHYEWWRRILKYFWMSVVVYTMLVLVFIYTYQFKAFPNLWKNMTNMNDDQLKDLGLEQYSTAELFTRIFIPTSFLLVCILHLHYFHDRFLQLTDLKAIVRKQDNAIYSHAKVNGRVYLIINRWVSLINYVFVIAWAFALPYSQFRPLASGVCTVWTCVIIICKMLYQLESINVTSFSISCPMPNTTEDQENLNESLLYKKPIDPTEWVGLRKSYPLLVYLRNNLLMLAILAFEVTVYRHQEYYRCHNNLTAPETKTIFHDITRLHLDDGIVNCLKYFVNYFFYKFGLESCFLLSVNVIGQRMDFYAMIHGFALIAVLYRRRRKAIAEIWPKYCCFLSCIITFQYFICIGIPPAPCKDYPWRNPNANFNSNIIKWLYFPDFIVKPNPVFLVYDFMLLLCASLQRQTFEDENKAAVRIMAGDNVEICMNLEAASFSQHNPVPDFIHCRSYLDMGKVVMFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGELLLKPIRSILRYWDWLIAYNVFVITMKNILSIGACGYIKSLINNSCWVIQAFSLACTVKGYDIPLDDPECKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDHIKARQQKYKKGKEKVLSMSEEASDVQEIQKVAEEDDDDEADKEKTKGKKKQWWRPWVDHASMVRSGDYYLFESDSEEEEEEEGKEEEEPPKRSAFQFLYQAWITDPKTALRQRRKEKKKNKKRQKVSDDVEIDMEGAERDEPVKSKSDGPDNIFKRIYNILKFTWVLFLATIDSFTAWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMKLSRESGLDEIYEPGQAERTASLESAASRDSESSCCTEATTLLLSRQSTLDDLDGPDPIPKTSERARPRLRKTYSMDMSSSSADSGSIVSSEATQVTMLYSRQGTAETIEEVEGEDHEGEDAEAEPIEEHEMEDYCMDSEGAAYISDSDMPSYSGLDGIAEPPPSYSKAVSLELFPLDSSQDDSSSRNQMMVSPDENQSEKPEDSILPPLTHELTASELLLNKMFQDEELDESEKFYSGQPRHLLLIYALYNTLVARSEMVCYFVIILNHMISASTITLVLPILIFLWAMLSVPRPSKRFWMTAIIYTEVTIVIKYFFQFGFFPWNKHLELKKDRPYFAPNIIGIEKKEGYVHYDLVQLLALFFHRSILKCHGLWDEEDGGDNGSNKDDSDDELAESYRRDSADSLTSVNLAASVESIHVHFPEQQTAIRRKSSSSGSHLSQRSSFSSHRSKRGSTSTRNSSQKGSSVSSIKRKSKKELLMEKLREQFIIAKAFTIKKTLQIYLPIRQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPEAFLVMVLIQFGTMVVDRALYLRKTVMGKVIFQVILVFGIHFWMFFILPIVTDRKFSQNIVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVTNRPLDVSITITLGGYQPIFTMSAQQSQLQNMTNRQYKDLLRAFNKDTGALQFLENYAIEDITVAQLEGSSNSLWTISPPSRKNMIKELKADNPDFSLVISWNIQRNISLGAKAETVSDKISHNLPADTRKKIAAMMENPNQHINHSVMLAGVYPHYIKAPSDNAAKPIKQLLEGGKFKDITVSLSKNNESAQLHEWWVLNQKNKFLESNKTRLELIVFSDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2T6</accession>
    <name>A0A6I9X2T6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein C20orf24 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106538626</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908636.1">
        <property type="nucleotide sequence ID" value="XM_014053161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2T6"/>
    <dbReference type="GeneID" id="106538626"/>
    <dbReference type="KEGG" id="tsr:106538626"/>
    <dbReference type="CTD" id="55969"/>
    <dbReference type="OrthoDB" id="286395at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010742">
        <property type="entry name" value="RCAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12906:SF0">
        <property type="entry name" value="GEL COMPLEX SUBUNIT OPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12906">
        <property type="entry name" value="PROTEIN C20ORF24 RAB5-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908636.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16305" checksum="DDA621ACE8FD8609" modified="2020-10-07" version="1">MSGGRRKEEPSSHLHSQQQQQHQHLVANGGGGVLRASLWGKALRSDSAWEDKDEFLDVIYWFRQIIAVILGVIWGIVPLKGFVGIAIFCLINAGVLYLYFSSFQQIDEEEYGGTWELTKEGFMTSFALFLVVWIIFYTAIHYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2Y7</accession>
    <name>A0A6I9X2Y7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bardet-Biedl syndrome 10 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BBS10</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908202.1">
        <property type="nucleotide sequence ID" value="XM_014052727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2Y7"/>
    <dbReference type="GeneID" id="106538279"/>
    <dbReference type="KEGG" id="tsr:106538279"/>
    <dbReference type="CTD" id="79738"/>
    <dbReference type="OrthoDB" id="9393833at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042619">
        <property type="entry name" value="BBS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14667">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14667:SF2">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013908202.1"/>
        </source>
    </evidence>
    <sequence length="711" mass="79848" checksum="FD2C990B64C6B311" modified="2020-10-07" version="1">MATSARLELGRLAQEAAALAGVVRGSLGPHGGQVLLTRPTGEVLLSRDGRRVLEALNVDSPTARIMIACISTHCNLFGDGAKTFIILLSDLLRQFEKLNKRDQGKYCSLKHISQFLVKIQTNILDHIMTQDLKKHFWSIFSASASDTRRNMELVLEPYFCGKVGSNRQMFLTHLTCDFYFKVTAGKNQNEALYLVNECFAELHTTVTGLPFSDSRILDGLLLHRDFAVYCPTEGDKKIIIITEPIHSSLSELGIEVVITAENQFKASETWITKRTETLLQHMQDNDIKVILSGVKQHDIVHRYGKNNGISIVDCLLTEEISFICKITGISPFKPSLDNINHEITQTAVAKFCQPFHLGTKRFVHIGFAKTSTIQLYCMILCGPVHGVTEQHASAFREAFKMLQQMFTAIHLSQNCDSELENHSLLNSLNKTQQHSSPHQTLFQEHNYWRTFQANTKHLAPCGLKMEKHSASFSVVDENTLHSSSYNTSTIVMPSVDLMHGIKSSLFQKRDDDLVDCQTASLKPKVQKERLQMTENELLEDKQPVFNIVDKNVQLQVSTARCARLLKYEENYQRGNQNYSDSCIELGSVLPVGGLFEILLHYYLSNYAKQCQSSNTSIICSILADVLLSIPKTLCMTQKRNAFPQLCLQVTSALKSNQQLLPNQKFLESVSCKYHLIASVLQCAAQLLSVDLIISIKRLPQKAEESDSEVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3N8</accession>
    <name>A0A6I9X3N8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein phosphatase Slingshot homolog 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106538816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013908916.1">
        <property type="nucleotide sequence ID" value="XM_014053441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3N8"/>
    <dbReference type="GeneID" id="106538816"/>
    <dbReference type="KEGG" id="tsr:106538816"/>
    <dbReference type="OrthoDB" id="5779068at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043587">
        <property type="entry name" value="Phosphatase_SSH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043588">
        <property type="entry name" value="SSH-N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE SLINGSHOT HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864">
        <property type="entry name" value="SLINGSHOT PROTEIN PHOSPHATASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23040">
        <property type="entry name" value="PH_SSH1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Slingshot N-terminal" evidence="2">
        <location>
            <begin position="76"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908916.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15267" checksum="129A8E24C3EB9C2B" modified="2020-10-07" version="1">MALVTVQRSPTPSATSSPCASEADSGEDDCRFQPRSISESFLTVKGAALFLPRGNGSSTPRVNHRRNKHAGDLQQHLQAMFILLRPEDYIRLAVRLESSYQNRTRYMVVVSTNGRQDTEESIVLGVDFSSNDSTVCTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6N3</accession>
    <name>A0A6I9X6N3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Activator 1 subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RFC5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909502.1">
        <property type="nucleotide sequence ID" value="XM_014054027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6N3"/>
    <dbReference type="GeneID" id="106539267"/>
    <dbReference type="KEGG" id="tsr:106539267"/>
    <dbReference type="CTD" id="5985"/>
    <dbReference type="OrthoDB" id="10254700at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000028">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000004">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF9">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="91"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013909502.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="42808" checksum="C458D02D2141A634" modified="2020-10-07" version="1">MGFTGDFFITSTRSVNFPILFPPQCGNSKRGRHLEERALGKPRSKHGFSSCENLHFIFIGRVEKYRPQTLGDLISHEDILNTIQKFISEDRLPHLLLYGPPGTGKTSTILACAKQLYTGREFSSMVLELNASDDRGIDIVRGPILNFSSTRTIFKRGFKLVILDEADAMTQDAQNALRRVIEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMVPRLKHVIEKENVDVSDDGMKALVTLSNGDMRRSLNILQSTYMAFGQVTEETVYTCTGQPLKSDIANILDWMLNLDFSSAYRNIMELKTLKGLALQDILTEIHLFVHRVDFPTSVRIQLLIKMADVEHRLATGTNEKIQLGSLVAAFQVTRDLIVAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC58</accession>
    <name>A0A6I9XC58_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytosolic phospholipase A2 gamma-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106540727</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911432.1">
        <property type="nucleotide sequence ID" value="XM_014055957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC58"/>
    <dbReference type="GeneID" id="106540727"/>
    <dbReference type="KEGG" id="tsr:106540727"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF39">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2 3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="4">
        <location>
            <begin position="48"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013911432.1"/>
        </source>
    </evidence>
    <sequence length="577" mass="66757" checksum="14324D7C8BEA7DF4" modified="2020-10-07" version="1">MQCRFQTDEVYLRLGLANVSYTLVTREETMQVNADPGASQISEESAKVDLAFICDIRISRDISNHEKQHVEARKRIVQKCLQKFHIECPKDNFPTVAVLGSGGGLRALIALQGTLRELQEHNMLDTVMYLCGVSGSTWCMSYIYENEEWGKQLKSLEDKLCERLVDPHQDWKKILKILKNLIEPSKDDTYSLTDFWGYFVAYIILNELDDDHHLSDEQTICKNGIVPYPIYAAVDATKLDDANRFHSDIWFEFTPHESGFFNPGAFVDVTYLGSKFNNGKLKEKKNEKKMTYLREYRCSCDCCKVALAIIKFFLYTMESQTCMMENKTHADVKKHIINITEILENKGPSDTYNFCCTLKEIDYSLDLTENGKHILKLVETLQLEFTDIWDIVTFMHKSLACILTWKWGNISNYTYKYIDLKDKNLTDKTNIQLADAGLVINSAYPLILRPERKVEFILSFDFSEGDPFETIKEASQYCKENKLPFPEIDARNLDKDKDNPEDCYIFRSNGCPTVMHFPLFNKVNCEGHVSDWRNRYTTFNKTSYPKQDIQNLLEAAKINVRNNAEKIRQTLKSVANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEJ9</accession>
    <name>A0A6I9XEJ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">YLP motif-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106541639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912616.1">
        <property type="nucleotide sequence ID" value="XM_014057141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEJ9"/>
    <dbReference type="GeneID" id="106541639"/>
    <dbReference type="KEGG" id="tsr:106541639"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023334">
        <property type="entry name" value="REKLES_domain"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51486">
        <property type="entry name" value="REKLES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="REKLES" evidence="2">
        <location>
            <begin position="295"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912616.1"/>
        </source>
    </evidence>
    <sequence length="343" mass="36401" checksum="2DB0186BF658C8D7" modified="2020-10-07" version="1">MFPTWGLYGATPGHYPPPPTMLLPPPPLPGVLPTSMPPPPPQVPVVPPPVLGGYSTSSAATSSSSGTGGFLSLQEQHLVQLQQLQQMHQKQLQSVLLGGPPPPGPPPPSMPPPLPASSAANTSWQPPIVAGPPAARSYQKQFKHRDPLPPPLPRDSQFRNPPMAPLPSTDEVAEPSQPRKEWEPVAAPASPCMDMDMDLSSPPQSPPQSFLPQVEADPYVPPPQTLPAQSQIPPPPQAYQPLLPLSQAVAKTFSDTPPPTVSQSYPNQSQTYQANSQPSTQLGQPLATSAPQPESHFTSSQITQSASEPPELRLSSGPEKECEPQQSSSSADLSAMTSQVRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFC9</accession>
    <name>A0A6I9XFC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PAPSS1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914374.1">
        <property type="nucleotide sequence ID" value="XM_014058899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFC9"/>
    <dbReference type="GeneID" id="106543012"/>
    <dbReference type="KEGG" id="tsr:106543012"/>
    <dbReference type="CTD" id="9061"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050428">
        <property type="term" value="P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002194">
        <property type="entry name" value="3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF17">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="5">
        <location>
            <begin position="51"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013914374.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="15081" checksum="09AB2DEED4B6FA14" modified="2020-10-07" version="1">MDLPTSQCKKVKLSNKVQSWGMQRATNVTYQAHHVSRNKRGQVVGTRSGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFE4</accession>
    <name>A0A6I9XFE4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RING-box protein 2</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
            <ecNumber evidence="17">2.3.2.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">RING finger protein 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Sensitive to apoptosis gene protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">RNF7</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.32"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Catalytic component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes) composed of CUL5, Elongin BC (ELOB and ELOC), RNF7/RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component. Also interacts (with lower preference) with CUL1, CUL2, CUL3, CUL4A and CUL4B; additional evidence is however required to confirm this result in vivo. Interacts with UBE2F. Interacts with CSNK2B, the interaction is not affected by phosphorylation by CK2. May also interact with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="EC" id="2.3.2.32" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013912896.1">
        <property type="nucleotide sequence ID" value="XM_014057421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFE4"/>
    <dbReference type="GeneID" id="106541867"/>
    <dbReference type="KEGG" id="tsr:106541867"/>
    <dbReference type="CTD" id="9616"/>
    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031466">
        <property type="term" value="C:Cul5-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061663">
        <property type="term" value="F:NEDD8 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16466">
        <property type="entry name" value="RING-H2_RBX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000156">
        <property type="entry name" value="RING-box protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="62"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013912896.1"/>
        </source>
    </evidence>
    <sequence length="114" mass="12704" checksum="629548E64061393E" modified="2020-10-07" version="1">MADVEDGDEPGISTSHSGSSGAKAGGADKMFSLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQAENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG77</accession>
    <name>A0A6I9XG77_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nischarin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NISCH</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914669.1">
        <property type="nucleotide sequence ID" value="XM_014059194.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543235"/>
    <dbReference type="KEGG" id="tsr:106543235"/>
    <dbReference type="CTD" id="11188"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06875">
        <property type="entry name" value="PX_IRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001442">
        <property type="entry name" value="Nischarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000020">
        <property type="entry name" value="nischarin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000102">
        <property type="entry name" value="nischarin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037904">
        <property type="entry name" value="Nischarin_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057714">
        <property type="entry name" value="PH_NISCH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF35">
        <property type="entry name" value="NISCHARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25625">
        <property type="entry name" value="PH_NISCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="9"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="461"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="486"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="514"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013914669.1"/>
        </source>
    </evidence>
    <sequence length="1415" mass="159446" checksum="9128EDBAB866F2ED" modified="2020-10-07" version="1">MEALRTGEEGEEPPKSIRILGSELVETYTVYIIQVTVGTHEWIVKHRYSDFHDLHEKLVLEKKIDKNILPPKKIIGKNSKSLVEKRQKELEVYLQTLLAIFPLAAPKVLSQFLHFHLYEVIGITAALAEELFHKGEQLLVAGEVFNIKPLQLYAVTQQLRQGKPTCANGDARTDLGHILDFTCRLKYLKITGTDGPVGSSNIQEHLLPFDLSIFKSLHQIQINHCGAKLINGLTSSKHTLATLSVQFSATSMMEILVPEAYEFDQWEPEGTSSNPVTAVIPTWRTLTTLDMSHNRIAQIDNSVKLMPKIEFLDLSHNDVPLVENLQHLYNLIHLDLSYNKLSTLEGIHTKIGNIKTLNLAGNHLERLSGLNKLYSLVNLDLSNNKVDQIDEIRNIGGLPCLEKLVLANNPLSIIPDYRTKVLAQFGDRASEVCLDNTVTTEKELDTVEVLKAIQKAKDVKYKMTNSDKKIPEDSKLSATSSKSNCSSLSVHPSSPSLPRPVSSSQEITHKKTALTRSFSSSSLTPKSHTVTQRCPETPDSCSASQVSAPDLDFSDKSTHRMCSGYPEEGPDSLPDSLNTVTLPFTCMSYATTNQDFVQHLSTLIAEAVGNVPLFSVETENMGDLLSDSGVAETEDGYFEMGLHEGYQISKVTPTSSGVQMTQAKQDNVIKILWSFCIQVHKGLRQFASCFVLASNVIAVFEIVQDSKGNCQHILSALKVVLCFPYSDLSEFGFLIPEICLMLNINNGETCLCVVSDTQNLQDFYSCLHQFRSQRYKPALTGSVLYCGKASLQEFVYQLMGFYHISSENTEIKGCFPVYLFCHHKADGQKEQCLPELGNDGKTCCKHCGCSGFYSAIHKSAGESHCMLHPCWIFLTPHHLHIVKVDFNILPGKFMDSENARNIFKLNRIPLASVLLHPTCYSVQQEGSFSDGYVLELIIGFRFVTAIFVLPHEKFHFLRIYSFLRTLLQDIKTIIIFKDANHVESVRKSLTDCLRHGPTTSFCKPRLPFSSLYPSEFLMQRITDENQIPMHLSVSPSLQYVAGLKEKYIMEFFHNSIAEVENEELRHLMWSSVLFYKALDMEVTACVLLSTKAIYFLLDDSVTHSNDHHLGFWGTDVLGYEFISFHLTPCLVLKLNDLHSVNIGLFDQYFRLTGHSAEHILTCLTRDSYSTHAFIQHLMAVLSLLARTPSPEPVDKDFYSEFGKKNTGKMENYELIHSSRVRFIYPNEEEIGDLAFLVAEKMKSVMKSPSLNLLLYVLAFQVNITEGIAQAYSSLQPKTLILTSSDLFLFNEDYISYPRPEFAKEPPKRDKYQLADGRRIRDLDRVLMGYQAYPQALTFVFDDIQNHDLMQNLTLDHFGNVFSFPKGQAAQEGNVSQEVLWCVFIPSPDSREKLISLLARQWEILCGRELPLELTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG91</accession>
    <name>A0A6I9XG91_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12 13">COBW domain-containing protein 1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12 13 14" type="primary">LOC106543246</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914683.1">
        <property type="nucleotide sequence ID" value="XM_014059208.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914684.1">
        <property type="nucleotide sequence ID" value="XM_014059209.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914686.1">
        <property type="nucleotide sequence ID" value="XM_014059211.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013914687.1">
        <property type="nucleotide sequence ID" value="XM_014059212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG91"/>
    <dbReference type="GeneID" id="106543246"/>
    <dbReference type="KEGG" id="tsr:106543246"/>
    <dbReference type="CTD" id="55871"/>
    <dbReference type="OrthoDB" id="258627at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="8">
        <location>
            <begin position="39"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="CobW C-terminal" evidence="9">
        <location>
            <begin position="301"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013914683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013914687.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="42276" checksum="483D8BA096E231A7" modified="2020-10-07" version="1">MESSFIDDEEDCPELIPIKEPAIKDNVEDIQSSNNFKIPVTIISGYLGAGKTTLLNYILTEQHSKRIAVILNEFGEGSALEKSLAISQGGELYEEWLELRNGCLCCSVKDNGLKAIENLMQKKGKFDYILLETTGLADPGSVASMFWVDAELGSDIYLDGIISVVDAKHGLQHLKEEKPTGLINEAARQVALADLIIINKVDLVSQAELMKLRTAICTINGVANILETQRSRVDLSNVLDLHAFDGVSGKSLQQKLQLEEEPRPHLDKSIVTSTFEVLGSATEEDLNVFIQNLLWEKNVKDKKGNPMEVIRLKGLVSIKDKSHQVIVQGVYELYDLEETTVKWKEDERINRLVLIGRNLDNDILKDLFIANVTKIEENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGE9</accession>
    <name>A0A6I9XGE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Gamma-aminobutyric acid type B receptor subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">GABBR1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Postsynaptic cell membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910113.1">
        <property type="nucleotide sequence ID" value="XM_014054638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGE9"/>
    <dbReference type="GeneID" id="106539761"/>
    <dbReference type="KEGG" id="tsr:106539761"/>
    <dbReference type="CTD" id="2550"/>
    <dbReference type="OrthoDB" id="17569at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15291">
        <property type="entry name" value="7tmC_GABA-B-R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06366">
        <property type="entry name" value="PBP1_GABAb_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000031">
        <property type="entry name" value="gamma-aminobutyric acid type B receptor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000055">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000072">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002456">
        <property type="entry name" value="GPCR_3_GABA_rcpt_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF77">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01177">
        <property type="entry name" value="GABAB1RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="14 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 24" id="KW-0732">Signal</keyword>
    <keyword evidence="21" id="KW-0768">Sushi</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026900807" description="Gamma-aminobutyric acid type B receptor subunit 1" evidence="24">
        <location>
            <begin position="25"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="600"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="717"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="841"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="26">
        <location>
            <begin position="102"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="25">
        <location>
            <begin position="607"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="874"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="925"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="874"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="941"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013910113.1"/>
        </source>
    </evidence>
    <sequence length="994" mass="112933" checksum="2B03A817241442ED" modified="2020-10-07" version="1">MVRRKKMLLLLVVSLLAIAQLGHGAVHLNITEGCKLIFPPWDGGIRYRGLTREQVKTAHFLPFDYEIEYVCRPEREIVGPKVRKCLPNGTWTDSDVESRCLRTCPKMHLNLENGQVSVRAIERVPVEGTWVEYRCNPGFYLLGSPRSNCTKVGRWSSAKPICELWRPPVSRSGSGKKRLHIGALFPMTGGWPGGQACLPSAQMALDDVNNRRDILPDYELRLDYRDSECEPGVATKFMYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVQLFKKWSWTKIATIQQTTEVFTSTLDDLEERVKEAGIEITFRQSFFSDPTVPVKNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYADNWFRIKDANINCTEEEMKEAVEGHITTEIVMLNPENTRSISNMTSEEFIEKLTKRVEKSPEETGGFQEAPLAYDAIWALALALNKTSAELVKKGLRLEDFNYNNQTITDEIYRALNSSAFEGVSGHVVFDASGSRMAWTLIEQLQDGVYKKIGYYDSTKDNLSWYNNDKWIGGVPPADHTKVIITFRYLSQKLFISVSVLSSVGILLAIICLAFNIYNGHVRYIQNSQPYLNNMTAVGCALAQAAVFPLGLDGLHISKDLFPFVCQARLWLLGLGFSLGYGSMFTKIWWVHTVFTKKEEKKERRKTLEPWKLYATVGLLVGLDFVILAIWQIVNPLKRTTEEFTKEEPKSDIDVLILPKLEHCSSEKMNTWLGIFYGFKGLLLLLGIFLAYETKGVSTEKINDHRAVGMAIYNVAVLCLITAPVTMILSSQQDAAFAFASLAIVFSSYITLVVLFVPKMRRLITRGEWQSEQQDTMKTGSSTNNNEEEKSRMLEKENRELEKIIAEKEERVSELQQQLKERQQLRSRRRSSNCAADNHFNNSASASAATGPNLYQPSLPLVRCLVAEQADKLGRNNCDGSRVHLLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHQ2</accession>
    <name>A0A6I9XHQ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartate--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Aspartyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">DARS</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013910518.1">
        <property type="nucleotide sequence ID" value="XM_014055043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHQ2"/>
    <dbReference type="GeneID" id="106540056"/>
    <dbReference type="KEGG" id="tsr:106540056"/>
    <dbReference type="CTD" id="1615"/>
    <dbReference type="OrthoDB" id="372395at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000144">
        <property type="entry name" value="Aspartate--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000013">
        <property type="entry name" value="Aspartate--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="18">
        <location>
            <begin position="196"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013910518.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="57047" checksum="FADA7F88BF010812" modified="2020-10-07" version="1">MPGVRKNQERNSDRPMEPPPADDYAKERYGILPMIQSQEKPDRVLVRIKDLTGEKADETVWVRGRIHTSRAKGKQCFLVIRQQQFNIQALVAVGDHASKQMVKFAANINKESIVDIEGVIRKVPNKIGSCTQQDVELHIQRIYVISLAEPRLPLQLDDAIRPEVEGEEEGRATVNQDTRLDNRVIDLRTSTSQAVFRVQSGVCQLFRETLIHKGFVEIQTPKIISAASEGGANVFTVSYFKTSAYLAQSPQLYKQMCICADFDKVFCIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVVDEIADTLVQIFKGLQERFQTEIQTVCKQFPCEPFKFLEPTLRLEYQEALAMLRKAGVEMGDEDDLSTPSEKLLGRLVKEKYDTDFYILDKYPLAVRPFYTMPDPANPITSNSYDMFMRGEEILSGAQRIHDPELLTERALHHGIDLEKIKSYIDSFRFGAPPHAGGGIGLERVTMLYLGLSNVRQTSMFPRDPKRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHW3</accession>
    <name>A0A6I9XHW3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Calcium-binding mitochondrial carrier protein SCaMC-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106542466</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913676.1">
        <property type="nucleotide sequence ID" value="XM_014058201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHW3"/>
    <dbReference type="GeneID" id="106542466"/>
    <dbReference type="KEGG" id="tsr:106542466"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000028">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000003">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002167">
        <property type="entry name" value="GDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00928">
        <property type="entry name" value="GRAVESDC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027043872" evidence="15">
        <location>
            <begin position="19"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="218"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="312"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="409"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="23"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913676.1"/>
        </source>
    </evidence>
    <sequence length="503" mass="56255" checksum="A13698ED03B14638" modified="2020-10-07" version="1">MLSSFWRLFSSFFLNAGCEGPAKEDAAKEEAPLPHPGDPQAPKMPQKLHNGKEADPSERRPTILLVVGPAEHFTEKIVKAGDKDLDGQLDFEEFVHYLQDHEKKLRLVFKSLDKKNDGRIDAQEIMQSLRDLGVKISEQQAEKILKRIRTGHSWGPVTYMDRNGTMTIDWNEWRDYHLLHPVENIPEIILYWKHSTIFDVGENLAVPDEFTIEERQTGMWWRHLVAGGGAGAVSRTCTAPLDRLKVLMQVHASRSNNMCIVGGFSQMIREGGPRSLWRGNGINVLKIAPESAIKFMAYEQIKRFIATDQEVLRIHERLVAGSLAGAIAQSSIYPMEVLKTRMALRKTGQYTGMLDCAKNILAKEGLGAFYKGYVPNMLGIIPYAGIDLAVYETLKNSWLQHYAVNSADPGVFVLLACGTMSSTCGQLASYPLALVRTRMQAQASIEGAPEVTMRSLFKHILKTEGAFGLYRGLAPNFMKVIPAVSISYVVYENLKMTLGVQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI31</accession>
    <name>A0A6I9XI31_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SHC-transforming protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SHC3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915409.1">
        <property type="nucleotide sequence ID" value="XM_014059934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI31"/>
    <dbReference type="GeneID" id="106543844"/>
    <dbReference type="KEGG" id="tsr:106543844"/>
    <dbReference type="CTD" id="53358"/>
    <dbReference type="OrthoDB" id="9938362at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01209">
        <property type="entry name" value="PTB_Shc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000036">
        <property type="entry name" value="SHC-transforming protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051235">
        <property type="entry name" value="CEP152/SHC-Transforming"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006019">
        <property type="entry name" value="PID_Shc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337">
        <property type="entry name" value="SHC TRANSFORMING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337:SF4">
        <property type="entry name" value="SHC-TRANSFORMING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00629">
        <property type="entry name" value="SHCPIDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="165"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915409.1"/>
        </source>
    </evidence>
    <sequence length="437" mass="48409" checksum="0889F2F2AF1DFFB8" modified="2020-10-07" version="1">MLQRSKYNRFRNDSVTSVDDTVHNWSINSKLSSTDTISASSPYLASPENLQKEHGGSTTLSTLVPKASHLKLSSTGSLWGIKNLSSAVKELAVAKLQGSSAAETSDNTCSLATATLPSQQDKNKMNTGKKPWSEEMYLGDEDWNQRGSTANKVPQGWLHSNEKILGPGVMYIVKYLGCIEVLRSMKSLDFNTRTQITREAIGLVCEAVPGTKGAFKRRKPPSKALSNILGKTNLQFAGMNISLNISTTSLNLMTPDCKQIIANHHMQSISFASGVDVDTTDYVAYVAKDPVNHRACHILECCDGLAQDIISTIGQAFELRFKQYLHCPSKIPALQDRMQSLDELWMEEDSEVAEHPYYNSFPNKMDERVKVKIPPISDPAQSASALGKEQTYYQSQHVRDLCDEDRQQAPSRQGSLDIYSLPEGKSNVDLSKEMPMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK08</accession>
    <name>A0A6I9XK08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxysteroid dehydrogenase-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106544403</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916134.1">
        <property type="nucleotide sequence ID" value="XM_014060659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK08"/>
    <dbReference type="GeneID" id="106544403"/>
    <dbReference type="KEGG" id="tsr:106544403"/>
    <dbReference type="OrthoDB" id="5327538at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09762">
        <property type="entry name" value="HSDL2_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000301">
        <property type="entry name" value="Hydroxysteroid dehydrogenase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051935">
        <property type="entry name" value="HSDL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006133">
        <property type="entry name" value="PRK08278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808:SF3">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="10">
        <location>
            <begin position="346"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013916134.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="47509" checksum="AB9FD3AC2258B28D" modified="2020-10-07" version="1">MLPNTGKLAGCTLFITGASRGIGKAIALKAARDGANVVIAAKTGVPQRTLPGTIYTAAEEIEAAGGKALPCIVNVREEDQIADAVVLAVQKFGGIDVLVNNASAISLTGTLETPTKKVDLMMSANTRGTYLTSKLCLPHLRKSKIGHILNISPPINLNPIWFKNHCAYTISKYGMSMCVLGMAEEFRGEVAVNALWPQTAIYTSAMDMLGGAGVEKQCRKTDILADAAYCILSKPKSFTGNFIIDEVLLREEGIKNFDVYAVAPGYPLIPDFFLDVDPDTIAMKMEAHGASPTFKEGKKQDIVKNEGPDKTKAEGPDRDKLHVHFAEDKPAGPVAETFKLIKGMINEDIVKATQGVYQFELSGEEGGTWYIDLKNKGGSAGIGEAPGKVDVIMNMSGNDFVKMFSGKLKPTMAFMSGKLTIRGDVALAIRLEKLMAQFNARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKJ0</accession>
    <name>A0A6I9XKJ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sperm-associated antigen 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SPAG1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916319.1">
        <property type="nucleotide sequence ID" value="XM_014060844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKJ0"/>
    <dbReference type="GeneID" id="106544548"/>
    <dbReference type="KEGG" id="tsr:106544548"/>
    <dbReference type="CTD" id="6674"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000221">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051982">
        <property type="entry name" value="CiliaryAsmbly_MitoImport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025986">
        <property type="entry name" value="RPAP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45984">
        <property type="entry name" value="RNA (RNA) POLYMERASE II ASSOCIATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45984:SF3">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13877">
        <property type="entry name" value="RPAP3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="660"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-polymerase II-associated protein 3-like C-terminal" evidence="7">
        <location>
            <begin position="839"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="349"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916319.1"/>
        </source>
    </evidence>
    <sequence length="963" mass="109701" checksum="B736C4E30700587F" modified="2020-10-07" version="1">MMLQKLPKLRVFINDWEGRISISCWYWYLCFNSWNNNVFGWLSNILFILRSGEEGLFPELILFCENCIESLAPNSRVLRKEKAVANASDFTTEEWKKINDDLTSWITEMKEDEYKTQYATTEVFKENVENLPPIRSSENHLSLSQNETVKIKQKKKNIPRDYKEWDKFDVEKECSKIEDQENDYSKTSINNNIKLPKAEIDTIGMTTKEKTFAAHREKEKGNEAFVIGDYKEAIAYYSRSISALPTVAAYNNRAQTEIKLQNWHNALQDCEAVLKMEPENIKALMRRSTVYSNLQNFEMAAEGLKKVLHIDPKNAIAKKKLLDIDKNLKESEPISQTQSKGKRIFIEEIEESDEQERNEGEFVNGSGDRKTAVLVGRESTSEPADMGNVQKKFSSRGGAWKPDHKEAQKPRDPSENNVKKGGLEETIREPLQDDKKEINGYLSNCTNGNEIGEGKPKDVESSAAGKSNSTLLPANAAKLKTEGNELFKNGQFGEAMLKYSRAIENVLSSGVQAPEDLSILYSNRAACYLKEGNCADCIQDCNSALELHPHSIKPLLRRAMAYESMERYQQAYVDYKTLLQIDSGIQAANDSVNRITRTLIDLDGPNWREKLPPIPAVPFSAQLHWWHGESFTSESKQKKTANTCRNQLSQTPSDNAEEKFKLFKTQGNEFVKKGKYEEALMKYNECIKLNSQEKTIYTNRALCFLKLLKYEEAKKDCDYVLQIDGSNIKALYRRALAYKGLENYEASIEDLNKVLLIDSSIGEAQKELDEATQLFKLRREFTANNQPKQRKKIEIQEVNEEEEDTETCSSNTAINPCNAKEELGTTMSLNSSEKLTISKPSNAYEFGQIINIVNASKDIAACAELLAMIEPKDLPVLLSNKLEGDIFLIIIQALQSDVFCQNPNLVYQHLFYLSKAERFKVVQTLLGKNEIEQVQQLLASLSNVRDQPFSSNDLENLKKTFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLK1</accession>
    <name>A0A6I9XLK1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide exchange factor MSS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Rab-interacting factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RABIF</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Guanine-nucleotide-releasing protein that acts on members of the SEC4/YPT1/RAB subfamily. Stimulates GDP release from both YPT1, RAB3A and RAB10, but is less active on these proteins than on the SEC4 protein. Might play a general role in vesicular transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with RAB8A.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914787.1">
        <property type="nucleotide sequence ID" value="XM_014059312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLK1"/>
    <dbReference type="GeneID" id="106543326"/>
    <dbReference type="KEGG" id="tsr:106543326"/>
    <dbReference type="CTD" id="5877"/>
    <dbReference type="OrthoDB" id="30840at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00246">
        <property type="entry name" value="RabGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.10:FF:000004">
        <property type="entry name" value="Guanine nucleotide exchange factor MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007515">
        <property type="entry name" value="Mss4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276:SF0">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04421">
        <property type="entry name" value="Mss4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51796">
        <property type="entry name" value="MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914787.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="14349" checksum="BC703E31A92EC6CA" modified="2020-10-07" version="1">MAAPSSAMEAAALGPEELVCASGRNRKAVLCQRCGSRVLLPGAATFTRRELFLPAMKKKTALVTNGDLEGDVLQDHWLVEDMFSFENVGFTKDVGNVKFLICADCEIGPIGWHCLDDKKSFYVALDRVSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM01</accession>
    <name>A0A6I9XM01_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C15orf57 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106544977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916889.1">
        <property type="nucleotide sequence ID" value="XM_014061414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM01"/>
    <dbReference type="GeneID" id="106544977"/>
    <dbReference type="KEGG" id="tsr:106544977"/>
    <dbReference type="CTD" id="90416"/>
    <dbReference type="OrthoDB" id="5982503at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028039">
        <property type="entry name" value="CCDC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31800">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31800:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14989">
        <property type="entry name" value="CCDC32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916889.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="25477" checksum="88E471867434D076" modified="2020-10-07" version="1">MGENPSRCRTGAITWEDHCRRPSVSVSPDQCYFGNAPLLNCCIMIMTESVDSLVAVSSQDLWTEICSSLPHPDQNEESNNAFADSFADSYIHVMENQMEVTGPSLQVNEKPWVPLKDSELYLASLERRLMRIKGLSQEVTSKDILHTLSQAKKESWDRFLQEKSESDFYLEGTDSDESTLEYLKRWLQPEKVAVSSEEIQYLIPLGAPPEKQEAGEDEEPAPVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNE1</accession>
    <name>A0A6I9XNE1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">A disintegrin and metalloproteinase with thrombospondin motifs 18-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106541369</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912268.1">
        <property type="nucleotide sequence ID" value="XM_014056793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNE1"/>
    <dbReference type="GeneID" id="106541369"/>
    <dbReference type="KEGG" id="tsr:106541369"/>
    <dbReference type="OrthoDB" id="9020539at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF256">
        <property type="entry name" value="PEPTIDASE M12B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="12">
        <location>
            <begin position="186"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912268.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="48572" checksum="F2C6CAFEA105EC23" modified="2020-10-07" version="1">MLQKGVLPVSQVLVSKLKILQLCCLGCGDVVAVLPGDSGGLSTFNQDYIFVIPVEVDSSGSYLSHDIWHSARRKRSTVSSKASLHYRFSAFGEELHLELKPSDFLSHNVTVQVLGKDGIEDSQVRDIEQCFYQGFIRNYSSSSVAISTCTDSPKPPTEDTFILSDEYGSQARLKRSPIKNQKGGSLNVETLVVADKKMLEKHSKENVTTYILTVMNMVSSLFKDGTIGSDINIVVVSLLLLEQEPGGLVINHHADQSLNSFCQWQSALVGKNGKRHDHAILLTGFDICSWKNEPCDTLGFAPISGMCSKYRSCTINEDTGLGLAFTIAHESGHNFGMIHDGEGNPCRKAEGNIMSPTLIGNNGVFSWSVCSRQYLNKFLNTAQAACLIDEPKQAGQYKYPDKLPGQIYDADTQCKWQFGAKAKLCNLSFVKLLALSVCLRCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRD3</accession>
    <name>A0A6I9XRD3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">RNF19A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RBR family. RNF19 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013916321.1">
        <property type="nucleotide sequence ID" value="XM_014060846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRD3"/>
    <dbReference type="GeneID" id="106544550"/>
    <dbReference type="KEGG" id="tsr:106544550"/>
    <dbReference type="CTD" id="25897"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20362">
        <property type="entry name" value="BRcat_RBR_RNF19A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20355">
        <property type="entry name" value="Rcat_RBR_RNF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16775">
        <property type="entry name" value="RING-HC_RBR_RNF19A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000001">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000004">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000052">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="335"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="388"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="102"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="106"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="595"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="791"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="617"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="645"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="678"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="795"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013916321.1"/>
        </source>
    </evidence>
    <sequence length="811" mass="87531" checksum="5A8837A18FE14C87" modified="2020-10-07" version="1">MNVAAMSLHRQMGSDRDLQSTASSVSLPSIKKAPKKRRISLGSLFRRKRDTKHKAKDVNGGVDGIASIETIHSEICPDKTSILSAYMSSDSGTAICSKQTGDFIECPLCLLRHSKERFPEIMTCHHRSCVDCLRQYLRIEISESRVNISCPECSERFNPHDIRLILNDDILMEKYEEFMLRRWLVADPDCRWCPAPDCGYAVIAFGCASCPKLMCGREGCGTEFCYHCKQIWHPNQTCDAARQERAQSLRLRTIRSSSISYSQESGAAADDIKPCPRCAAYIIKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYLSPSGCTFWGKKPWSRKKKILWQLGTLVGAPVGIALIAGIAIPAMIIGIPVYVGRKIHNRYEGKDMSKHKRNLAIAGGVTLSVIVSPVVAAVTVGIGVPIMLAYVYGVVPISLCRSGGCGVSAGNGKGVRIEFDDENDITVSGTNAAADTTSVAEARHNPSIGEESVGLTGSLSASGSHMDRIGAIRDNLSETASTMALAGASITGSLSGSAMVNCFNRLEVQADVQKERCSLSGESGTVSLGTVSDNASTKAMAGSIINSYVPLDRDGSSMEVQVDIESKPAKFRHHSGSSSVDDGCAAGRSNTGSSSACLSDNKSSVPKWSKESSAGKKCKGKLRKKNSTKINETREDMDAQLLEQQSTNSSEFDSPSLSDSVPSVADSHSSHFSEFSCSDLESMKTSCSHGSSDYHTRFNTVSILPEVENDRLENSPHQGSSLLFTPIAPNSEVPQLSYIAEECTCKGTSNTAGVNIGETLKERNNNHSPQVTELSTSLQTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRN5</accession>
    <name>A0A6I9XRN5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxynucleoside triphosphate triphosphohydrolase SAMHD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SAMHD1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:46149"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>dATP + H2O = 2'-deoxyadenosine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17256"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61404"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:67649"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>dCTP + H2O = 2'-deoxycytidine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80083"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:80084"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>dGTP + H2O = 2'-deoxyguanosine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61429"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:15194"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>dTTP + H2O = thymidine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80079"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:80080"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAMHD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907385.1">
        <property type="nucleotide sequence ID" value="XM_014051910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRN5"/>
    <dbReference type="GeneID" id="106537715"/>
    <dbReference type="KEGG" id="tsr:106537715"/>
    <dbReference type="CTD" id="25939"/>
    <dbReference type="OrthoDB" id="9991235at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09508">
        <property type="entry name" value="SAM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000015">
        <property type="entry name" value="Deoxynucleoside triphosphate triphosphohydrolase SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000067">
        <property type="entry name" value="SAM and HD domain-containing deoxynucleoside triphosphate triphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2760:FF:000002">
        <property type="entry name" value="SAM and HD domain-containing deoxynucleoside triphosphate triphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="15" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SAM" evidence="25">
        <location>
            <begin position="46"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="26">
        <location>
            <begin position="166"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="589"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013907385.1"/>
        </source>
    </evidence>
    <sequence length="621" mass="71495" checksum="DA36A417D9A1384A" modified="2020-10-07" version="1">MSNGSREGALKRAWPTSSADGFQTPQKRGVAAADSIGSISLHPRSWDTEQLCAFLQAQGLEAEELQRFREHEITGETVPSLTESHLEKMGICTVGVREKLLICLRKLAQSQSYTEIAKVFNDPIHGHIEMHPLLVRIIDTPQFQRLRYIKQLGGTYYVFPGGSHNRFEHSLGVSHLAGRLVQALQERQPELNIDQRDILCVQIAGLCHDLGHGPFSHMFDARFIPLARPGLKWKHELASVQMFEYLIDSNNLKPDMEKYGLQLDEDIDFIKEQIASPVEEKMGDKEWPYCGRSKNKSFLYEIVANKTSGIDVDKWDYFARDCHHLGIQNNFDYERFIKFARVCQVKDMMHICTRDKEVGNLYDMFHTRNCLHRRAYQHKVSNIIETMITDAFLKADPYIKIKGSGGKMFRISTALEDMEAYTKLTDEIFLKILHSDEPELSDAREILCKIQQRDLFKCLGETQPPEGKEIKKEDYDGLRAEIVNSKPKTISVDVELKAEDFIVDVIDMDYGMKKRNPINSVRFYCKTDITQAVKITREQVSQLLPEKFAEQIIRVYCKKKDAKIIEAATKYFIQWCMDKDFTKPRDGDVVAPELTPMKKGWNKDDENQDSNRNSSCKVKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS53</accession>
    <name>A0A6I9XS53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 850-like isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106542344</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913523.1">
        <property type="nucleotide sequence ID" value="XM_014058048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XS53"/>
    <dbReference type="GeneID" id="106542344"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000056">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000218">
        <property type="entry name" value="Zinc finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000098">
        <property type="entry name" value="Zinc finger protein 614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000495">
        <property type="entry name" value="zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000180">
        <property type="entry name" value="Zinc finger protein 689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000862">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001016">
        <property type="entry name" value="zinc finger protein 850-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="34"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="398"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="593"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="621"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="649"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="677"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="705"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="733"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="761"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="789"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="817"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="845"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="873"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="901"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="929"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913523.1"/>
        </source>
    </evidence>
    <sequence length="954" mass="110847" checksum="9610FDD35C039CB1" modified="2020-10-07" version="1">MGERPYECPDCGKSFKENSYLVIHRRIHTGEKPYECPDCGKGFNWNSELKIHQRTHTGEKPYECPDCGKGFSRNSNLVKHQRTHPGQKPFECPDCGESFSRNSDLVIHQRTHMGERPYECPDCGKNFKENFKLVIHQRIHTGEKPYKCPDCGKGFNWNSELEVHQRTHSGEKPYECPDCGKGFSRNSNLVKHQRTHTAEKPYQCLDCGKGFNWNSELEVHQRTHTGEKPYECPDCGKGFSWNSNLVKHQRTHTGEKPYECPDCGKSFSDNSYLVKHYGNHTGLKPFECSDCGKGFSSNSELVIHQRIHTGEKPYKCLECGKHFIQNSHLVKHQRTHTGEKPYECPDCGKHFTRNSDMVIHQRIHTGEKPYKCADCGKHFTRNSDMVKHQKTHTEEKPYKCDCGKSFSQNSYLVKHQRIHTGEKPYRCADCEESFHRNSDLVVHQRTHTGDKLYKCLDCGKIFTRNSNLMMHQLTHTGEKLNECLFCGKSFFQNSNLVIHQRTHTGEKPYECPDCGKSFRQSSHLVRHQKTHTGEKPYECLDCGKGFNWNSDLVIHLKTHKGEKLYECPECGGKFSCKSELVKHQKTHKERRLYECLDCGKSFLHNSKLVLHQRTHTGEKPYKCLECGKCFFRNYDLVIHRRTHTGEKPYECLECGKCFSRNYDLVIHRRTHTGEKPYECPDCGKDFSTRTSLLNHVRIHTGEKPYECPDCGKSFNWNSDLEVHQRTHTGEKPYECPDCGKSFSRNSNLVKHQRTHTGEKPYECPDCGKSFSRNSDLVRHQRSHTGEKPYKCLECDKCFIQNSHLLIHQKIHTGEKPYECPDCGKRFNRNSDMVIHKRTHTGEKPYECPDCGKGFSWNSNLVKHQRTHRGEKPYECPDCEKCFSQNSYLVKHQRTHTGEKPYECLECGKSFSWNYNLVTHWRTHTGIKPYECPDCGKDFSCRSSLLNHVRLHIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTT0</accession>
    <name>A0A6I9XTT0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein LOC106538220</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106538220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908140.1">
        <property type="nucleotide sequence ID" value="XM_014052665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTT0"/>
    <dbReference type="GeneID" id="106538220"/>
    <dbReference type="KEGG" id="tsr:106538220"/>
    <dbReference type="OrthoDB" id="6083769at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051586">
        <property type="entry name" value="PKC-binding_NELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24042:SF8">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24042">
        <property type="entry name" value="NEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027028809" evidence="5">
        <location>
            <begin position="26"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="332"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="6">
        <location>
            <begin position="332"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="412"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="6">
        <location>
            <begin position="412"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="351"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013908140.1"/>
        </source>
    </evidence>
    <sequence length="899" mass="99463" checksum="6B45B2CA4B66AE77" modified="2020-10-07" version="1">MQVKQPYWTCCCWLGVCLWLLAGEGGCVYEKLCGIAPCGRNTNGGLQPSRQQCHGARCFSRSNRSSLSFIQPSSKQQPHQSSRGSILGLLAVTGRGTDVICSGGRDCPRRILNSTMQDCRGIECRLPLHIRQKPRPASQISPCPPDQGERCLEPTSARVTEKAAQFIEEELAYTASELGGASLGVQLTCDVKPGGNEVPSEDALVLQLQLTKGQEQFVETLKSQQAIIVDLQQKLTEQQDALVTQQREIILQQRKMYEQMDLIKVQYGMLFDTVKQMSFKGLQEDIQHYFEANLQNLQKEVRNHLQKSYSGHKLNVDAKMIDIGETLMECGFCESDEFCNFQKTPSQCEKCTLCPAGFFQLLDCSTNADRICQDKDECTQLPSICGERMKCLNTPGGFRCFGMAEKEAALGLCGEEYFFNKEQQECQACLVCDDGTVILPCSLVSDTVCSTAVETLLSETWAADTALPLSGSSQIYPGFKLKLKEKQGCNMGTLEEGSLVFKDHGLIWADFNFAVKHNCRNFLHLSLKFKDSEEGYELSGVRIEQPEVKLFQSASVSCAAEVEPSQTLSIFLKSPNQFCNQSKDLDIYNLQTPLSLFWLSHDTGAVAMTAQIMTAVHYQTNYQPTFKFVSLSDSYMLTLCHDGRAIKFTETGVVKFVFQQALYSMGHTCIHEGFSMVVYLNKNGTNAELMTVYKSGVNYRDTSISVSGATKVSAGDLMSFEILSPAQCSVRYFGDSSGISTLSLIWLPSAISTALSARVSVIGLPTGAVRNKLLDFVQVSIYEKQIYLISAGQFAQKYFVFTEEGIASIVFTLKLIHSCNMLRVTLNQHISDNAPLNIIAQQIGGQVPEGSIWASVSLRSSFKVHNGTMISVSLDCVRGRINHIAHEYGTSLSILWISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTV2</accession>
    <name>A0A6I9XTV2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16 17">Homeobox protein Hox-B3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16 17 18 19" type="primary">HOXB3</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917475.1">
        <property type="nucleotide sequence ID" value="XM_014062000.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917476.1">
        <property type="nucleotide sequence ID" value="XM_014062001.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917477.1">
        <property type="nucleotide sequence ID" value="XM_014062002.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917478.1">
        <property type="nucleotide sequence ID" value="XM_014062003.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917480.1">
        <property type="nucleotide sequence ID" value="XM_014062005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTV2"/>
    <dbReference type="GeneID" id="106545448"/>
    <dbReference type="KEGG" id="tsr:106545448"/>
    <dbReference type="CTD" id="3213"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000094">
        <property type="entry name" value="Homeobox protein Hox-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025281">
        <property type="entry name" value="DUF4074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF11">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13293">
        <property type="entry name" value="DUF4074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="171"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="173"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="65"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="294"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="356"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="91"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013917475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013917476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013917477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013917478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013917480.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="44344" checksum="973B32ADFF2010F1" modified="2020-10-07" version="1">MQKTSYYDTSPLFGAYSYPGANGFGYEGPSPQPFQPAAHVESDYQRPACTLQSLGNAAPLAKAKDLNGSCLRPSLPQEHHPPPPVSPPTNPITATSGSGSTTSNSTGGSNNHSQSGAVKSVPSKSSLTSSNAALTKQIFPWMKESRQNAKQKNSSPCAESCSGEKSPPGSSASKRARTAYTSAQLVELEKEFHFNRYLCRPRRVEMANLLNLSERQIKIWFQNRRMKYKKDQKSKGLGSSSGGPSPTGSPPQALQSSAGFLNALHPMTSNYEAPSPPSFNKPHQNAYAMPTAYQNPIKGCPSQQKYANTAPEYDPHVLQGNGSGSYGPPNMQGSPVYVGGNYVDSMPNSASSLYGLNPLQHHQPPSMEYNGGPQMASNQHHGPCEAHPTYTDLSSHHASTQGRIQEAPKLTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTZ3</accession>
    <name>A0A6I9XTZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Monocarboxylate transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Solute carrier family 16 member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106538275</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(R)-3-hydroxybutanoate(out) + H(+)(out) = (R)-3-hydroxybutanoate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71795"/>
            <dbReference type="ChEBI" id="CHEBI:10983"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:71796"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:71797"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-lactate(in) + H(+)(in) = (S)-lactate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29415"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:29416"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:29417"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-methyl-2-oxobutanoate(out) + H(+)(out) = 3-methyl-2-oxobutanoate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71783"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>4-methyl-2-oxopentanoate(out) + H(+)(out) = 4-methyl-2-oxopentanoate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71779"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>acetoacetate(out) + H(+)(out) = acetoacetate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71775"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:71776"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:71777"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908200.1">
        <property type="nucleotide sequence ID" value="XM_014052725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTZ3"/>
    <dbReference type="GeneID" id="106538275"/>
    <dbReference type="KEGG" id="tsr:106538275"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015650">
        <property type="term" value="F:lactate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000030">
        <property type="entry name" value="monocarboxylate transporter 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004743">
        <property type="entry name" value="MCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00892">
        <property type="entry name" value="2A0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF24">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="356"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="20">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="394"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013908200.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="47204" checksum="DA504156F2E5C1FF" modified="2020-10-07" version="1">MLAVMYAGGPISSILVNKYGSRPVMIAGGCLSGCGLIAASFCNTVEELYFCVGVVGGLGLAFNLNPALTMIGKYFYKKRPLANGLAMAGSPVFLSTLAPLNQYFYSIFGWRGSFLILGGLLLNCCVAGSLMRPIGPKPDQVKKEINKEALQEIGKGSVKETGDVNTDLIAGKNKKQKTTVYQTINKFLDLSLFTHRGFLIYLFGNVFMFFGLFTPLVFLSNYGKSKHFSKESSAFLLSILAFVDMVARPSMGLLANTKWIRPRVQYFFAVSVIYNGVCHLLAPLSTTYAGFCIYAGFFGFAFGWLSSVLFETLMDLVGTQRFSSAVGLVTIVECCPVLLGPPLLGKLNDIYGDYKYTYWACGIILIFSGIFLFIGMGINYRLVAKEQKAMDKQIAEGREEETNIDEAEKQKESNLPPKTTEDGIKEDSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUF5</accession>
    <name>A0A6I9XUF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Selenocysteine lyase</fullName>
            <ecNumber evidence="10">4.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17" type="primary">SCLY</name>
    </gene>
    <organism evidence="13 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.16" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013908333.1">
        <property type="nucleotide sequence ID" value="XM_014052858.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908334.1">
        <property type="nucleotide sequence ID" value="XM_014052859.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908335.1">
        <property type="nucleotide sequence ID" value="XM_014052860.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908337.1">
        <property type="nucleotide sequence ID" value="XM_014052862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUF5"/>
    <dbReference type="GeneID" id="106538390"/>
    <dbReference type="KEGG" id="tsr:106538390"/>
    <dbReference type="CTD" id="51540"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009000">
        <property type="term" value="F:selenocysteine lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000083">
        <property type="entry name" value="Selenocysteine lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000065">
        <property type="entry name" value="Selenocysteine lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF62">
        <property type="entry name" value="SELENOCYSTEINE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="12">
        <location>
            <begin position="35"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013908333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013908334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013908335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013908337.1"/>
        </source>
    </evidence>
    <sequence length="447" mass="49319" checksum="E0ED58E29930639E" modified="2020-10-07" version="1">MEETTLKMILVEQRTETNETLNCNGKNNISSSRKIYMDYNATTPLAPEVIETVKEAMYEAWGNPSSSYEAGQRAKKIINEARENLAKMVGGRFQDIIFTSGGTEANNLVIHTAVKDFMKSSKQSPVATKESHEEKTLGTLPHIIISCIEHDSVRLPVEHLVKEQKAEVTFVPVSKVTGQVEIDDVVAAIHPNTCLVSVMLANNETGVVMPISELSHQIEVINHKRKASGLPWILVHTDAAQMIGKGRVDVQDLGVDYLTIVGHKFYAPRIGALYVRGLGMNIPLHPMLFGGGQEWSFRPGTENTPMIAGLGKAAELVNENSKLYEAHMRNVRDYLEERLETIFGRQNIHFNCRLKGSKRLCNTCNFSILGSGLQGWLVLSRCRTLQASVGAACHSEKGDQPSAVLLSCGIPYNVAQNAIRLSVGRDTSKEDVDLIVEDLKRAVAELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUN9</accession>
    <name>A0A6I9XUN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor Spi-C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SPIC</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis. Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Binds DNA as a monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914378.1">
        <property type="nucleotide sequence ID" value="XM_014058903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUN9"/>
    <dbReference type="GeneID" id="106543015"/>
    <dbReference type="KEGG" id="tsr:106543015"/>
    <dbReference type="CTD" id="121599"/>
    <dbReference type="OrthoDB" id="10043646at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000335">
        <property type="entry name" value="Spi-C transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPI-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="11">
        <location>
            <begin position="17"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914378.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="19244" checksum="0219192DC498A1C3" modified="2020-10-07" version="1">MYTTSLQQKSNRGRKRLRLFEYILESLHNPEMANCIQWIDKANGVFQFISKNKEKLAEQWGERKGNRKVMTYQKMARALRNYGRTGEITKIRRKLTYQFGAVVLRRLSLACFSGKDGNIGDQFVKAEQGCLYAADWFTYQNCINQKGCDLQQDSDLTSSFYCEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUX1</accession>
    <name>A0A6I9XUX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TTC27</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TTC27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914458.1">
        <property type="nucleotide sequence ID" value="XM_014058983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUX1"/>
    <dbReference type="GeneID" id="106543073"/>
    <dbReference type="KEGG" id="tsr:106543073"/>
    <dbReference type="CTD" id="55622"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="530"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="598"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="818"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013914458.1"/>
        </source>
    </evidence>
    <sequence length="846" mass="96965" checksum="5655CBE6E09EA12A" modified="2020-10-07" version="1">MRALERVLLRGFPSAAESPRWKQSEAASAAESGSLLQLLLEGNYESIFLSKAVQNVFTTSSGKEDTIESNLEKQIQAYLDCSTADIDSIERQSVVFLLGVGSLQLFAQSNWTGPPPDLQPQDFLPPEILQQYNETSALSTAILGMMVLDGESIYSLASQPILLMLARVILVNSRNKLEAIQTLPWWTLRCINLHQQLLEEKSPELFAVAQACIQQVFEIETLFNDDECWHLAVQFHLECAYTFLNYYEYKKAKEHFLAAKDITKLQIELTGALGKRTRFQEKYVAQLILDVQRKDNIILPYRELSPAPTPLEDLTKNYALDDDTVLNEIKFEESHQNQMPDLCAEELAVILGICIDFQKNNPVHKLTEEELLAFTSYLLSQPKFWAIQTSALLLRTKLEKGSIRRMERAMKQTQALADQFEEKTTSVCERMKIFYSCQVPPHWAIQRQLASLLFDLGCTTSALQLFKKLEMWEDAVICYERAGQHGKAEEILRQELEKKETVGLYCLLGDVLKDHSYYDKAWELSNHRSARSQRSKGLLYLRNRNFRECIECFEHSVKINPMQLGVWFSLGCAYLALEDYEGASRAFQRCVMLEPDNAEAWNNLSTAYIRLKQKIKAFRTLQEALKCNYEHWQIWENYILTSTDIGEFSEAIKAYHRLMDLKEKYKDVQVLKILVKVVMDEVGDRNGETAKGLKGKLQALFGRVTSRVTNDGEIWRLYARLYENGQNENYEDIEKALQYLTKAYKCEIQSGDWDKDASSFKKVARDALELAHVAMKCSKLKTNYQEALPILSSARLNLRGLVAKAKQNFADAVSDEVSSDLVEEMAAMENLMMELQELSNQLRNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWL2</accession>
    <name>A0A6I9XWL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="10">1.1.99.2</ecNumber>
            <ecNumber evidence="3">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">L2HGDH</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the L2HGDH family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="10"/>
    <dbReference type="EC" id="1.4.3.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013918171.1">
        <property type="nucleotide sequence ID" value="XM_014062696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWL2"/>
    <dbReference type="GeneID" id="106545963"/>
    <dbReference type="KEGG" id="tsr:106545963"/>
    <dbReference type="CTD" id="79944"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008726">
        <property type="entry name" value="PRK11728.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF2">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="12">
        <location>
            <begin position="18"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013918171.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="47700" checksum="A449665183B6CE1E" modified="2020-10-07" version="1">MPSRPLSADNGRRRSTFDVVVIGGGIVGLASARELSLRHPSLAFAVLEKEKELALHQSGHNSGVIHSGIYYTPGSLKAKLCVEGAVLCYEYCDQKGIPYKQCGKLIVAVEHEEIPRLKALYERGTQNNVRGLQLISPKEIQAKEPYCRGLMALDSPYTGIVNYRQVALAYAEDFQTAGGTVLTEFEVEQMDMAKESAEGSKEGMKYPIIVRNSKGKEVRCRHLVSCAGLYSDRLSQISGCDPEPRIVPFRGDYLVLKPEKCYLVRGNIYPVPDPRFPFLGIHFTPRMDGSVWLGPNAVLAFKREGYKFYDFSIQDFIDAAAYSGLWKLALKNFSYGMGEMYRACFLNAQLKQLQRIIPEVTINDIQRGPAGVRAQALDSGGNLIDDFVFDSGTGDIAARILHVRNAPSPAATSSLAIAKMIAKEVEQRFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXB2</accession>
    <name>A0A6I9XXB2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Epidermal growth factor receptor substrate 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EPS15</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918802.1">
        <property type="nucleotide sequence ID" value="XM_014063327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXB2"/>
    <dbReference type="GeneID" id="106546449"/>
    <dbReference type="KEGG" id="tsr:106546449"/>
    <dbReference type="CTD" id="2060"/>
    <dbReference type="OrthoDB" id="524326at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF54">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918802.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="62329" checksum="475612EDD7F6DBB6" modified="2020-10-07" version="1">MHLKRETGLGGWITVPGVTDVLKYSCLICWSSREKNNVEQDLKEKGETIKQKTSEVQDLQDEVRRESNNLQNLLTQKQEAEEILNGLEEKKAWLEEQLSMIRQKCTEEVDLITSLKKEIAEQESKISGYKDDLSKAQEELSRLQQEASELEKNVEEGKLQLAPLQQDLQDSQQDITLVQEKLLELKQLENSQFNWEAQPHGILINGNADHSSLSNSSSETVNLNENAERENPADSEQVNYETPARNSPETTHVDTEEEKEALPWDVTKKADLVNEDSHQPSDQTTEANLDFFESDPFVGNDPFKDDPFGKIDPFGSDPFKGSDPFAADNFFKQSSSDPFGNIDPFSATNNNSTTETLKHNDPFAPGGTTVTASSDPATDPFASLFGNESFGCDFADFSTLSKTNNEDPFNSSANVNNIVITKNLFEEAPVKNEDIPPALPPKTGTPTRPCPPPPGKRPIHKLESSESFKLNDPFQPFPSSDGSKGKEADPFAPSGVGKEMDSSNFANFNTYPTEEDMIEWAKRESEREEKERLARLKQQEQEDLELAIALSKSEISEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZK4</accession>
    <name>A0A6I9XZK4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cadherin-12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106539640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909940.1">
        <property type="nucleotide sequence ID" value="XM_014054465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZK4"/>
    <dbReference type="GeneID" id="106539640"/>
    <dbReference type="KEGG" id="tsr:106539640"/>
    <dbReference type="OrthoDB" id="9037817at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000097">
        <property type="entry name" value="cadherin-12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF96">
        <property type="entry name" value="CADHERIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cadherin" evidence="6">
        <location>
            <begin position="9"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="6">
        <location>
            <begin position="59"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="6">
        <location>
            <begin position="163"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909940.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25315" checksum="D6ED18BF4A99E1EE" modified="2020-10-07" version="1">MDNGCSNWPLDFETKKAYTFKVEASNVHLDHRFHSVGPFKDTATVKINVLDVDEPPVFSKPFYTMEVYEDTPVGTIIGAVTAQDLDIGSSAVRYFIDWKNDMDSYFTIDGMEGTIATNELLDRENTAQYNFSIIASKVSNPLLTSKVNVIINVLDVNEFAPEITVPYESYVCENARPGQVIQVIGAADKDLSPPGQKFSFRLSPEVTTKPNFTIHDFTKKKRQQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y091</accession>
    <name>A0A6I9Y091_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 13A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106544559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916333.1">
        <property type="nucleotide sequence ID" value="XM_014060858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y091"/>
    <dbReference type="GeneID" id="106544559"/>
    <dbReference type="KEGG" id="tsr:106544559"/>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056748">
        <property type="entry name" value="VPS13-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF22">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25037">
        <property type="entry name" value="VPS13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Intermembrane lipid transfer protein VPS13-like C-terminal" evidence="1">
        <location>
            <begin position="572"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916333.1"/>
        </source>
    </evidence>
    <sequence length="711" mass="80020" checksum="04E03F349B8B32FA" modified="2020-10-07" version="1">MAQSQWDLMTPFQVGAKHIFIASFFEGLQRIILFTEDENVFKMTYESEKVELAEQEIIVSLQDVGISLVNNYSRQEIAYIGITSSDIIWETKPKKKSRWKPLSVKQTDKLEKEFVEYCDNSPTENKIVELDDNIPQVCLTPTGNNMKILQPYEFPVRRSFLPALKVQYSTSEHQSSFRVQIYRIQIQNQIPGAIFPFVFYPIKPPKSISLDSEPKPFTDVSIVMRTAGHSQISRIKYFKVLIQEMDLRLDLGFLTALSEFFTHVDELPKDQEIELFKKDVESAQEELKNAVSTDTSQISLYEYFHISPIKLHLSFSLSTGREENNKDQKCKELIPVQSLNLLLKSIGATLTDVQDVVLKLAFFELNYHFCTTQGLQWAVIMHYSKQAIKQMYVLVLGLDVLGNPFGFIRDLSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASRITGAMAKGVAAITMDDDYQQKRREAMNKQPAGLKEGFTRGGKGLVSGFVSGITGIVTKPIRGAQKEGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRATNASEDVLSLRPPRFFSDDGVIRPYRLRDGAGSQMLQNIENGRFAKLRYFTHAMVNNTDLLMVTKSGILFVTRGTFGQLTCEWQYTYDEFTKKPFIIDGRRLRIEAKERVKSVFHAKEFGKIINFKTPEDAKWILSKLEEKVKEIQPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y137</accession>
    <name>A0A6I9Y137_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C5orf42-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013910460.1">
        <property type="nucleotide sequence ID" value="XM_014054985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y137"/>
    <dbReference type="GeneID" id="106540020"/>
    <dbReference type="KEGG" id="tsr:106540020"/>
    <dbReference type="OrthoDB" id="5974632at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028236">
        <property type="entry name" value="CPLANE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14492:SF4">
        <property type="entry name" value="CILIOGENESIS AND PLANAR POLARITY EFFECTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14492">
        <property type="entry name" value="JBTS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15392">
        <property type="entry name" value="Joubert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910460.1"/>
        </source>
    </evidence>
    <sequence length="885" mass="99499" checksum="2A68FD67F4058C55" modified="2020-10-07" version="1">MQIVGFANLPNTSQQMQQPQPFPLGRNWASSTEGNNIHRSPPVQPAGDVQIPTDHQIPTLENSWNVGQKKNSSQEEKNLPDQNQRSVHINDPSALRESQSVGARLMLPLQSSPHQNPARPFPLLTVSLNAEKKPKLIPLAKPLNNAEGFPLLKLKTSYQFQALNFCPVTPHRSSGPSSGPREAWGLPSSLAQNPPGLQMSEPTNETGAHLNLNRYDQNVTNQAPEQTKRWAEQVKTGISKQFPVDEYGIKEDSAPLQPCHERLRAEKPLVDHAINSHQAYGPFTEIPLLYLKTSPQSKCSLSTFTTKNTDCRASLGQTGLPLLHSNLPPLTKFQIPKLIPLQDLIAFEQSQHSVQYPQYKDHPKQMQLLKANIKVNETRQVRSNKKRQKRRIENRIKEKEEKIKPIVTNQQDDSTGLPDTVKPTKESQTKLSADPCDTNSLPISHPAKLSFTDLNELRNNESDTYISSAALHYLASVGKKTAELQDASTNTETELLPSCVPQTLPPELYFNFKSASATTETPLPSSSDLAEQRYISVTDIESGDLLKNLPVKSAPTAQPIVTQPVKSEFPASTKLYYPAVSFASVLPLDEFERQDKSPEMELSLLPPSVTEEENAGDPLTSSLLHEDFSSIYAGQKISREHFSGKLQEMDRQLSYLQNLTERMEKEYSTTKLFGETSKDVSVIPEQEEDDILFFAPGVQLCKAERYSTRVRVENFTDEKDFLEAESSPKDFIALKTPSVSPSGSAANRPRVNELFADISFKMEEQYERCSDDRLQITGFSDVLDIINDLIVENGVSPLELGLSEIQAKKIASLSNKPHKQTYKAEKDKKELHAWMKRKRKERLAEYMQTLSEKRAKEHNPFHLRKMPLGLSTREIKLQQKKKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1N3</accession>
    <name>A0A6I9Y1N3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear receptor-interacting protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NRIP3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910700.1">
        <property type="nucleotide sequence ID" value="XM_014055225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1N3"/>
    <dbReference type="GeneID" id="106540186"/>
    <dbReference type="KEGG" id="tsr:106540186"/>
    <dbReference type="CTD" id="56675"/>
    <dbReference type="OrthoDB" id="1047367at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12917">
        <property type="entry name" value="ASPARTYL PROTEASE DDI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12917:SF16">
        <property type="entry name" value="NUCLEAR RECEPTOR-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910700.1"/>
        </source>
    </evidence>
    <sequence length="273" mass="30663" checksum="14E4630CA0A75304" modified="2020-10-07" version="1">MFYSGLLTDRKDMDVREAAALRQQRRMKQAVQFNHKDSADLLPLDGLKKLGTSKDTQPHNILQRRLMETNMSRLRHSRGTCSQKNDPSAQTNKLNQMKLDSPPKTEEEDLIVICCQCNGKELQIVVDTGCQRNIISSACLERLGLKEHMKTCKAEGEKISLPHNMKAIGHLEHLPLIMGTVHVDCSAVVVAEGNEKSCSLGLQTLKYLKCVINLEKQNLILGKTDKEEIPFVNNASIHEEKAHPHGSHLRSWFSAVAHNWKPGKCPWKSTAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1T7</accession>
    <name>A0A6I9Y1T7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">HIG1 domain family member 2A, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">HIGD2A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920922.1">
        <property type="nucleotide sequence ID" value="XM_014065447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1T7"/>
    <dbReference type="GeneID" id="106548122"/>
    <dbReference type="KEGG" id="tsr:106548122"/>
    <dbReference type="CTD" id="192286"/>
    <dbReference type="OrthoDB" id="6604018at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050355">
        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF18">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="7">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013920922.1"/>
        </source>
    </evidence>
    <sequence length="102" mass="11292" checksum="B23E6BDB9F42145E" modified="2020-10-07" version="1">MAQNPPPPFDPYRPPVIEGFTPTSRGSTERFQSKFVRKFRENPFVPIGCLCTAGILTYGLICFINNKPKQSQMMMRARVIAQGLTVASLLVGMAVTNLKSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2G6</accession>
    <name>A0A6I9Y2G6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin T, slow skeletal muscle</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Slow skeletal muscle troponin T</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TNNT1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with TPM3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921247.1">
        <property type="nucleotide sequence ID" value="XM_014065772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2G6"/>
    <dbReference type="GeneID" id="106548410"/>
    <dbReference type="KEGG" id="tsr:106548410"/>
    <dbReference type="CTD" id="7138"/>
    <dbReference type="OrthoDB" id="330499at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031014">
        <property type="term" value="F:troponin T binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031444">
        <property type="term" value="P:slow-twitch skeletal muscle fiber contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000001">
        <property type="entry name" value="Troponin T, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF6">
        <property type="entry name" value="TROPONIN T, SLOW SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013921247.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="26767" checksum="BA93F5AEEBFD5055" modified="2020-10-07" version="1">MTRPVVPQLAPPKIPEGERVDFDDIHRKRMEKDLLELQTLIDVHFEQRKKEEEELVGLMSRIEHRRAERAEQQRVRTEKERERQAKLAEEKMRKEEEEAKKRAEDDAKKKKVLSNMGAHFGGYLIKAEQKRGKRQTGREMKIRILAERRKPLSIDNLGEQQLREKAKELHDWIHQLESEKFDLMEKLRRQKYEIRILYWPSMIGHTRNFKKGAGKTRLGGRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3L5</accession>
    <name>A0A6I9Y3L5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">T-complex protein 1 subunit zeta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CCT6A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911655.1">
        <property type="nucleotide sequence ID" value="XM_014056180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3L5"/>
    <dbReference type="GeneID" id="106540888"/>
    <dbReference type="KEGG" id="tsr:106540888"/>
    <dbReference type="CTD" id="908"/>
    <dbReference type="OrthoDB" id="10052040at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000038">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000029">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911655.1"/>
        </source>
    </evidence>
    <sequence length="530" mass="57631" checksum="E6E9CDC03355F637" modified="2020-10-07" version="1">MAVKALNPKAEVARAQAALAVNISAARGLQDVLRTNLGPKGTMKMLVSGAGDIKLTKDGNVLLHEMQIQHPTASLIAKVATAQDDITGDGTTSNVLIIGELLKQADLYISEGLHPRLITEGFEVAKEKALQVLEQVKVAKEMDREILLDVARTSLRTKVHAELADILTEAVVDSILAIRKPNEPIDLYMVEIMEMKHKSETDTSLIKGLVLDHGARHPDMKKRVEDAYILICNVSLEYEKTEVTSGFFYKSAEEREKLVKAERKFIEERVNKIIELKNSVVGDSNKGFIVINQKGIDPFSLDALAKEGIVALRRAKRRNMERLTLACGGIAMNSVDDLSIDCLGHAGLVYEYTLGEEKYTFIEKCENPRSVTLLIKGPNKHTLTQIKDAIRDGLRAVKNAIEDGCVVPGAGALEVAIADALVKHKPSVKGRSQLGVQAFADALLIIPKVLAQNAGYDPQETLVKVQTEYAESGQLVGVDLNSGEPMVAGAAGVLYNYSVKKQLLHSSTVIASNILLVDEIMRAGMSSLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y481</accession>
    <name>A0A6I9Y481_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cathepsin K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CTSK</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918618.1">
        <property type="nucleotide sequence ID" value="XM_014063143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y481"/>
    <dbReference type="GeneID" id="106546306"/>
    <dbReference type="KEGG" id="tsr:106546306"/>
    <dbReference type="CTD" id="1513"/>
    <dbReference type="OrthoDB" id="65740at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2250:FF:000003">
        <property type="entry name" value="Cathepsin L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026699422" evidence="7">
        <location>
            <begin position="20"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="8">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918618.1"/>
        </source>
    </evidence>
    <sequence length="161" mass="18580" checksum="B4FBC36C6466DFE8" modified="2020-10-07" version="1">MRPLVLLLATLLPVANVVAMESSLDTMLDSQWELWKKIHRKDYNGKMEEVPRRVIWEKNLKYINTHNLEFSLGRHTFELAMNHLGDMTSEEVVQKMMGLKVPPSRTQNNETLYIPDWEKKVPDSVDYRKKGYVTPVKNQGQCGSCWAFSSVGALEGQLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4D1</accession>
    <name>A0A6I9Y4D1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106541347</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912230.1">
        <property type="nucleotide sequence ID" value="XM_014056755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4D1"/>
    <dbReference type="GeneID" id="106541347"/>
    <dbReference type="KEGG" id="tsr:106541347"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="103"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013912230.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="41469" checksum="C6B8537F4391B3F1" modified="2020-10-07" version="1">MDARAPDGKKFIINEDAIQWHHKFEKVPPHSRCVKSCHQGFMKVVQEGAQICCYTCTKCSEGRISIQIDADQCERCPEDQYANARREQCLPKTFSYLSYSESLGVTLTSLTLFFSGILILVAVTFILHWDTPIVKANNRNITCILLSSLLLCFLCSLLFIGWPGEVTCFLRQTIFGTVFSISVSCILAKTITVVLAFMASKPGNQMRKWLGRRLAGSIIVICSFIQVVICVVWWTTFPPFPEFDMQSQMDKIIVQCNEGADFMFYIVLSYLGLLASVSFTVAFFARKLPDTFNEAKLITFSMLVFCSVWVSFIPAYLSTKGKYMVAVEIFSILASTAGLLGCIFLPKCYIILLKPHLNTKEHLSKRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4K9</accession>
    <name>A0A6I9Y4K9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Juxtaposed with another zinc finger protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">JAZF1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a transcriptional corepressor of orphan nuclear receptor NR2C2. Inhibits expression of the gluconeogenesis enzyme PCK2 through inhibition of NR2C2 activity. Also involved in transcriptional activation of NAMPT by promoting expression of PPARA and PPARD. Plays a role in lipid metabolism by suppressing lipogenesis, increasing lipolysis and decreasing lipid accumulation in adipose tissue. Plays a role in glucose homeostasis by improving glucose metabolism and insulin sensitivity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with NR2C2 (via ligand-binding region).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912385.1">
        <property type="nucleotide sequence ID" value="XM_014056910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4K9"/>
    <dbReference type="GeneID" id="106541452"/>
    <dbReference type="KEGG" id="tsr:106541452"/>
    <dbReference type="CTD" id="221895"/>
    <dbReference type="OrthoDB" id="3269380at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000619">
        <property type="entry name" value="juxtaposed with another zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003264">
        <property type="entry name" value="Juxtaposed with another zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051580">
        <property type="entry name" value="ZnF-Chromatin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057:SF0">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="90"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013912385.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="27094" checksum="9B19D1EBBCEA0816" modified="2020-10-07" version="1">MTGIAAASFFSNTCRFGGCGLHFPTLVELIEHIEDNHIDTDPRVLEKQELQQPTYVALSYINRFMTDAARREQESLKKKIQPKLSLTLSSSVSRGNVSTPPRHSSGSLTPPVTPPITPSSSFRSSTPTGSEYDEEEVDYEDSDSDESWTTESAISSEAILSSMCMNGGDEKPFACPVPGCKKRYKNVNGIKYHAKNGHRTQIRVRKPFKCRCGKSYKTAQGLRHHTINFHPPVSAEIIRKMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5D1</accession>
    <name>A0A6I9Y5D1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106541971</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106541971</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913040.1">
        <property type="nucleotide sequence ID" value="XM_014057565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5D1"/>
    <dbReference type="GeneID" id="106541971"/>
    <dbReference type="KEGG" id="tsr:106541971"/>
    <dbReference type="OrthoDB" id="9047411at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913040.1"/>
        </source>
    </evidence>
    <sequence length="342" mass="37439" checksum="237262470E5D85C0" modified="2020-10-07" version="1">MPITKHRPGLAGRPKGIGCWGDGELSKAVDEGEPAALEDLCWGPGVFSGLLGMVRGWAQSSRMQPACLFVVSVLSLASLRMSRVDASRDHLASFHPKHKKKITASPKDALKEEEEEEEEDKGFREQIVRRAVRKYPEDAEKHVEASHRQISIPTDKAEGSCPEVLAKGTLAVIVAALAFLGFFSTSVLLVVAVCILRIMDKQRKTGKEGKNGYPSSRRTLPPIPPPRPERTYMAMKPQGSDRLHPATPNTTEDAYLSVSELNPGPRTRQKLHYQNVSASSPPKAPIEKEYMTPDKVQPFSSPPSVKKYVTVEEPGPTPVTGGSPEIGTTPPGHDNHIYEQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5L2</accession>
    <name>A0A6I9Y5L2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Hemoglobin subunit alpha-D-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106547322</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the globin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919923.1">
        <property type="nucleotide sequence ID" value="XM_014064448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5L2"/>
    <dbReference type="GeneID" id="106547322"/>
    <dbReference type="KEGG" id="tsr:106547322"/>
    <dbReference type="OrthoDB" id="8751793at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031838">
        <property type="term" value="C:haptoglobin-hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005833">
        <property type="term" value="C:hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031720">
        <property type="term" value="F:haptoglobin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043177">
        <property type="term" value="F:organic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08927">
        <property type="entry name" value="Hb-alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000002">
        <property type="entry name" value="Hemoglobin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002338">
        <property type="entry name" value="Hemoglobin_a-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050056">
        <property type="entry name" value="Hemoglobin_oxygen_transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442">
        <property type="entry name" value="HEMOGLOBIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442:SF48">
        <property type="entry name" value="HEMOGLOBIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00612">
        <property type="entry name" value="ALPHAHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="8">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919923.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="15868" checksum="B3D9D72D2223A18A" modified="2020-10-07" version="1">MVLTAEDRRLLQASVAKLGCRLEDIGADALNRLFLAYPQTKIYFSHFNLSPGSKDIVHQGEKVGTALDSALKNLDDIRGPLSQLSDLHAYNLRVDPGNFELLSKCIHVSLATDLRNEYTASTFLAWDKFLEQVADILCENCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y663</accession>
    <name>A0A6I9Y663_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Apoptosis inhibitor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">API5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920423.1">
        <property type="nucleotide sequence ID" value="XM_014064948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y663"/>
    <dbReference type="GeneID" id="106547710"/>
    <dbReference type="KEGG" id="tsr:106547710"/>
    <dbReference type="CTD" id="8539"/>
    <dbReference type="OrthoDB" id="19224at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008383">
        <property type="entry name" value="API5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12758:SF19">
        <property type="entry name" value="APOPTOSIS INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12758">
        <property type="entry name" value="APOPTOSIS INHIBITOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05918">
        <property type="entry name" value="API5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920423.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17173" checksum="48A568854ABDF26D" modified="2020-10-07" version="1">MLLSSRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKSEVGQKRASEDTSSDLPAKKSQAGPKRDARQIYNPPSGKYSSNLGNFSYEQRGGFRSGRGRGWGGRGNRSRGRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6B5</accession>
    <name>A0A6I9Y6B5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106547792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920528.1">
        <property type="nucleotide sequence ID" value="XM_014065053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6B5"/>
    <dbReference type="GeneID" id="106547792"/>
    <dbReference type="KEGG" id="tsr:106547792"/>
    <dbReference type="CTD" id="113413377"/>
    <dbReference type="OrthoDB" id="6076093at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031746">
        <property type="entry name" value="DUF4732"/>
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    <dbReference type="PANTHER" id="PTHR37153">
        <property type="entry name" value="CHROMOSOME 19 C19ORF81 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37153:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC292874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15876">
        <property type="entry name" value="DUF4732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920528.1"/>
        </source>
    </evidence>
    <sequence length="264" mass="30040" checksum="93182FFE59DA5CD6" modified="2020-10-07" version="1">MATKVHQNETSPKKVIQNSGILEFEYPQLSSLEAESLETGIRMQEEENEGPRCQSRSSSQGECDLGSLVGQAKSYPDHHLGSAMANRRLLERAQADKKRGLQLSFSPLRRQYLKKIIAEYEALDMEMPCIRKFPRPPAARPLCLCLENPPEKEMTHAEILAAIEAVIPNAFEAGLLHSIQFENINVICGTAGRKNRWLITVSDFRTRNQLLCSGLTLGENHFVLRRWDNLVMEDYRMHLRRSLARQRLLDTLSDSWEASHLDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6D4</accession>
    <name>A0A6I9Y6D4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Parathyroid hormone/parathyroid hormone-related peptide receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">PTH/PTHrP type I receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Parathyroid hormone 1 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">PTH1R</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity. PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913940.1">
        <property type="nucleotide sequence ID" value="XM_014058465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6D4"/>
    <dbReference type="GeneID" id="106542665"/>
    <dbReference type="KEGG" id="tsr:106542665"/>
    <dbReference type="CTD" id="5745"/>
    <dbReference type="OrthoDB" id="6160250at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004991">
        <property type="term" value="F:parathyroid hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000070">
        <property type="entry name" value="Parathyroid hormone/parathyroid hormone-related peptide receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000005">
        <property type="entry name" value="Parathyroid hormone/parathyroid hormone-related peptide receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002170">
        <property type="entry name" value="GPCR_2_parathyroid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF27">
        <property type="entry name" value="PARATHYROID HORMONE_PARATHYROID HORMONE-RELATED PEPTIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00393">
        <property type="entry name" value="PTRHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027072574" description="Parathyroid hormone/parathyroid hormone-related peptide receptor" evidence="19">
        <location>
            <begin position="25"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="20">
        <location>
            <begin position="47"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="21">
        <location>
            <begin position="143"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013913940.1"/>
        </source>
    </evidence>
    <sequence length="546" mass="62440" checksum="AECD15F152924ED1" modified="2020-10-07" version="1">MGSHLICHGLRFILCCSALTSVFALVDPDDVVTREEQIMYLLEAKAQCERNLQEKGPQVPEGFCAPEWDGVICWPEGSPGKVVSIRCPDYIHDFYHEGYAHRQCDLNGSWALVPNQNVTWANYTECTKFLVLETRQNEVFHRLYLIYTVGYSISLGSLTIAVMILAYFRRLHCTRNYIHMHLFVSFMFRAISIFVKDAVLYSGSALEEMELIPEEDFKSMMEVSPMDNSTLYFSQYSPQVGCKLVVTLFLYFLATSYYWILVEGLYLHSLIFMAFFSEKKYLCGFTLFGWGLPAVFVTIWATLRATLADTECWDLSARYFKWFIQVPILTAVLANFILFTKIIKVLATKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMAMPYTEITGIIWKIQMHYEMLFNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFRRKARSGSTTYSYGPMVSHTSVTNVTAKGGVPLPLNGKPAPGTLNGHRNLPGYIRNGSLSENSLASSGPEHYSKEEEYLNSSGLYNGYRPATLLEKEEESVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6E1</accession>
    <name>A0A6I9Y6E1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transient receptor potential cation channel subfamily M member 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106542681</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913955.1">
        <property type="nucleotide sequence ID" value="XM_014058480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6E1"/>
    <dbReference type="GeneID" id="106542681"/>
    <dbReference type="KEGG" id="tsr:106542681"/>
    <dbReference type="OrthoDB" id="310870at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099604">
        <property type="term" value="F:ligand-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
        <property type="entry name" value="TRPM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041491">
        <property type="entry name" value="TRPM_SLOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF9">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY M MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18139">
        <property type="entry name" value="LSDAT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25508">
        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chromo" evidence="9">
        <location>
            <begin position="499"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913955.1"/>
        </source>
    </evidence>
    <sequence length="924" mass="105624" checksum="1285CB15B178573F" modified="2020-10-07" version="1">MKVFTYVYVYSDNQCKCGYLKSQHVEEMQINDNEKWNYKKHTKEFPTDAFGDIKFENLGKTGKYIRLSCDTDSETLYDLMTQQWHLKTPNLIISVTGGAKNFALKPRIRKIVSRLIYIAQSKGAWIFTGGTHYGLMKYIGEVVRDNTISRNSEENVIAIGIAPWGMVSNRENLIRTCDTETYYSASYIMDDQKKDLLYCLDNNHTHLILVDNGTHGCQGTETKLRTQLEKYISECIIPEVLTLDIVQRVLKDALTEQAVALELKIQENVSAAFKEVSEEILGIKELIQIHNQEIKEDIVEAFKGLSGYVSLLDDKVEEINQSNVNLDNWTKLLPFAEVAFNNTVHSSTGFTPFRIATGQDFSPMPEPTIPTIKEWMDTLQNIWPVVRLALQEAREAFKIQAEKKRMEPKPFHIGDRVYLSTKFLKSLQPLKKLGPNFIGPFPITRVINPVTVELLLPKTLRQVHPVFHISLLKQEVISSLRPPALTPPVPIMIEGEQHFEIKEMLDSRIHRRKTQYLVAWKHLPSSQNEWVAKHHVRPPTLLKKAINTAIKNKIPCVVVEGSGQIADVIASLTEAESTFTSSSVKERLLRYLPRTLSRLNEEEIESWIKCIKEILENPHLLTVIKIEDAGDEIVSNAISYALFKAFSTNEEDKDDWNGQLKLLLEWNQLDIASEEIFTNDRRWESADLQDVMFIALVKDRPKFVRLFLENGLNLRKFLTHEVLTELYAKNFSNLVFKNLQIAKNSYSDALLTFVWKMVEDFRKGIKKEDKNGKDDIEIQLLDGSLITRHPLQALFIWAILQNKKELSTVIWEQTRGCTLAALGASKLLKSLAKVKNDINAAGESEELANEYETRAVELFTECYSSDEDLAEQLLIYSCEAWGGNSCLELAIEAKDQQFIAQPGVQVIISILMILYPKTMDDLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8Q4</accession>
    <name>A0A6I9Y8Q4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">4-aminobutyrate aminotransferase, mitochondrial</fullName>
            <ecNumber evidence="6">2.6.1.19</ecNumber>
            <ecNumber evidence="5">2.6.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">(S)-3-amino-2-methylpropionate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">L-AIBAT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ABAT</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13993"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:58655"/>
            <dbReference type="EC" id="2.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:13994"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:23353"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 26">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="6"/>
    <dbReference type="EC" id="2.6.1.22" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013917330.1">
        <property type="nucleotide sequence ID" value="XM_014061855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8Q4"/>
    <dbReference type="GeneID" id="106545336"/>
    <dbReference type="KEGG" id="tsr:106545336"/>
    <dbReference type="CTD" id="18"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047298">
        <property type="term" value="F:(S)-3-amino-2-methylpropionate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000029">
        <property type="entry name" value="4-aminobutyrate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000191">
        <property type="entry name" value="4-aminobutyrate aminotransferase, mitochondrial"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 28" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="12 26" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00030857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00033650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013917330.1"/>
        </source>
    </evidence>
    <sequence length="509" mass="57465" checksum="182CCEA00469608E" modified="2020-10-07" version="1">MALMLLNRHTTWALQQSSRIFAGGFRGISQVAAKIDMEFDYDGPLMKTEVPGPRSRELMKQLNGIQNAEAVHFFCNYEESRGNYLVDADGNRMLDLYSQISSIPIGYSHPSLIKLLQQPQNLSTFVNRPALGILPPENFAEKLKESLLSVAPKGLSQVVTMSCGSCSNENAFKTIFMWYRVSKSYMDALGGWSLLKVGYRENIHFLFAFQPPGCPEYSMLSFMGAFHGRTMGCLATTHSKAIHKLDIPSFDWPIAPFPRLQYPLEDFVRENEQEEARCLEEVEDLIVKYRKKNKVVAGIIVEPIQSEGGDNHASDDFFRKLRNIARKHGCGFLLDEVQTGGGCTGKFWAHEHWGLEDPADVLTFSKKMMTGGFFHKEEFRANAPYRIFNTWLGEPSKNLLLAEVISVIKREDLLSNVTHAGKVLLMGLLELQSRYPHLISRARGRGTFCSFDTPNDATRNKLIEIARNKGVVLGGSGDRSIRFRPTLVFRDHHAHLFLNIFSDILAAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9H0</accession>
    <name>A0A6I9Y9H0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD repeat-containing protein on Y chromosome-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106546332</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918660.1">
        <property type="nucleotide sequence ID" value="XM_014063185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9H0"/>
    <dbReference type="GeneID" id="106546332"/>
    <dbReference type="KEGG" id="tsr:106546332"/>
    <dbReference type="OrthoDB" id="5980302at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF4">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="464"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="500"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="587"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="631"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="730"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="885"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013918660.1"/>
        </source>
    </evidence>
    <sequence length="1072" mass="122174" checksum="B66C4DFE37ACF277" modified="2020-10-07" version="1">MPLRKKVRPSSASGKLETSTLTSILNELTEPTLTKNSLFGRAQTSFAGPRLQTASPVSIGSFSEDRKNIPRPPEWLVRELLKRQQRSHSIGLMENISESESIHHRADSSRSQWKGKIEQHLTLDQLQKLYTAFQGLETSGHKSVDIESFKHILKTCVGSHNATDEEVEKLFMKIDYLATGNIQWHDFCTYLQIEYAELDSSSTRLKEVTFSLPAAMQEIPHGEPVLRICSLVDSTLITAQEDGQVSFWSPEFKLKRSKMVFEKIQRKSKWLMDFTVMTPYNKLILGTGDRELQFYESSNFEPYLQISGLEAVPLNLDYCYTDHDECMILYGDDQGCVNILQLYSVGETLRTWKRLPKVENLPNIRLESAILSSNVTYIRWKVHGDWVTQLNYYDSIKAIISASSHEPTALVIGCIVGATNVEQQMREIKENKKDSKMKKAQEMLGTPSQRAEGDQIIFRVHKGVKTFALSKKNNAIVTGGMDRIIRIWNPYMAGRPTGMLKSHMAPIFYVYVSEEDKIFSISTDNTVKIWDMEDQTCLFTACSKNSGIKGEITTCHYISGTRSLCVATDTLAILHLRLRSAPDLYTVTSHKKPVLCCKFNKVFRHVVSCSEDSVVKVWDFESGKPVFEFGNAHGDSAITCLTFDFSGRRLITGGKDGCLKIWNYNNGQCLHTLKKEDRNDEVRDCAYAEINKNEYIIGVGWDRRINLFYDSTDDFYHIKKPQPHWQDDINWGHKEDILCVAQCPPNLLATSSYDGEIIVWNMISGHIDHRFYIPMTTAASVHSQIDVTRIIFLKTRAVKFDSTTASLVSNGPQGYLHFWSLFSEDPLIASFQPSRGKSLISSIATTANDSYMYAADEDGYVYVHDIQNYALQDTKEETPKYVNHWRAHLGPVLTLEVIDEDNILLSGSIDCTIRLWSLNGEYIGTFGQEEPWEIFNTSSWKHPMVPYEILIDPQSMPIHPMLEDTSSNMQIISQDENKNLSKATCNPHSSQITDEDIEEEINKRLYSQASSRRMKYERSKLSSRWIKHRGPSIYHAIDYYEIDNFSGNCEKPDLSVLGVDIFRANYQGATEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAU4</accession>
    <name>A0A6I9YAU4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Digestive cysteine proteinase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106548217</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921030.1">
        <property type="nucleotide sequence ID" value="XM_014065555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAU4"/>
    <dbReference type="GeneID" id="106548217"/>
    <dbReference type="KEGG" id="tsr:106548217"/>
    <dbReference type="OrthoDB" id="65740at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000087">
        <property type="entry name" value="Counting factor associated protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026724247" evidence="7">
        <location>
            <begin position="19"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="9">
        <location>
            <begin position="244"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="8">
        <location>
            <begin position="328"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921030.1"/>
        </source>
    </evidence>
    <sequence length="547" mass="61770" checksum="1920DEAD98D92EA6" modified="2020-10-07" version="1">MGRLAFFLHLCAWTFAFGNMCSDTNKTPDFGGLYHVKGVISLPYAEIEEPFEAWYNLTGNKSRIEYYHGQVVTLQLGCLEPYGALFKITPATTENVVNMQKCFLLNGTKESKVKPQGVFPDMKDFKFVREEYYKGVYTAVWQSITDWGKKKNIYTMWVTNASCGVTPVHYEMKGYNNLLGSHYDKYEITYSDFSNSFPASIFVLKSNETKLCDELPGDSTEHHIMANPMADFVGGQEDRAHSLFHHYRKRFGKSYDTEQEMEHRKHTFTHNMRFVHSKNRANLPFKLALNHLADLTLEEMAVMRGKLKSTTPNKGLPFPEEIYTGLILPEILDWRLYGAVTPVKDQAVCGSCWSFSTTGSLEGALFLKTGHLISLSQQILIDCSWGFGNYACDGGEEWQAFEWILKHGGIATTESYGPYKGQNGYCHSNETHLVSKLSGYVNVTSGNTTALKAAIYKHGPVSVSIDASHRTFSFYSNGVYYEPKCKNKRGELDHAVLAVGYGVLQGELYWLIKNSWSTYWGNDGYILMSMKDNNCGVTTDAVFPIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAW1</accession>
    <name>A0A6I9YAW1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Twisted gastrulation protein homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">TWSG1</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the twisted gastrulation protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921114.1">
        <property type="nucleotide sequence ID" value="XM_014065639.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013921115.1">
        <property type="nucleotide sequence ID" value="XM_014065640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAW1"/>
    <dbReference type="GeneID" id="106548295"/>
    <dbReference type="KEGG" id="tsr:106548295"/>
    <dbReference type="CTD" id="57045"/>
    <dbReference type="OrthoDB" id="10037323at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030510">
        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006761">
        <property type="entry name" value="Tsg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057726">
        <property type="entry name" value="Tsg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057635">
        <property type="entry name" value="Tsg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12312:SF17">
        <property type="entry name" value="TWISTED GASTRULATION PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12312">
        <property type="entry name" value="TWISTED GASTRULATION PROTEIN HOMOLOG 1-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04668">
        <property type="entry name" value="Tsg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23782">
        <property type="entry name" value="Tsg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636635" evidence="7">
        <location>
            <begin position="25"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Tsg N-terminal" evidence="9">
        <location>
            <begin position="24"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Tsg C-terminal" evidence="8">
        <location>
            <begin position="86"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013921115.1"/>
        </source>
    </evidence>
    <sequence length="221" mass="24901" checksum="D487D1E868527D93" modified="2020-10-07" version="1">MRLWYFPGFLIISLLYFWIPSSMCCNKALCASDVSKCLIQELCQCRPGEGNCSCCKECMLCLGTLWDECCDCVGMCNPRNYSDTPPTSKSTVEELHEPIASLFRALTEGDTNLSWKIVSFPVAEELSPHENLVSVLETVNHPQHHNASISNNVHPPYSSEKERICTVVYFDDCLSIYQCKISCDSMGASKYRWFHNACCECIGPDCVDYGSKIVRCSNCMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAY8</accession>
    <name>A0A6I9YAY8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leukocyte receptor cluster member 8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LENG8</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921250.1">
        <property type="nucleotide sequence ID" value="XM_014065775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAY8"/>
    <dbReference type="GeneID" id="106548412"/>
    <dbReference type="KEGG" id="tsr:106548412"/>
    <dbReference type="CTD" id="114823"/>
    <dbReference type="OrthoDB" id="199574at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000002">
        <property type="entry name" value="Leukocyte receptor cluster member 8 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="685"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013921250.1"/>
        </source>
    </evidence>
    <sequence length="849" mass="95828" checksum="B5E1BCA148B675FA" modified="2020-10-07" version="1">MIMGIKVLANFFFLFHLFYLLFYRVSQYNLGTASTRENGMEGPMHENPEWEKARQALANISKANAAAASGNSKGATNGQANSQYSAQQGESLQQQQQYYQWYQQYNYLYPYNYYYPMQNVYSGYGSGQYGVSGSYSPTTQQPPVLGQHQGSMSQPPVPGMEDGGMSYSSQSQQMQISNPPQSSMQHSNHPSQHSNQPMGPGGQQQQQQQGGPAGGLHSSQSNQSSASYNQQQQQQQQQQHSYQEAAKPKKGQQLWNRMKQAPGSGGLKFNLPKRPFVVTNQNFGSGEQQQQQQPQQQQQQQQHPNFASQQNSEKRHNHSSSTGKPEDWPQDMKEYVQRCFTACESEEDKDRTEKLLKEVLQARLQDGTAYTIDWSREPLPGLGRDNLAESPKKKQQRWEVSSLHPPRASVQSSLAQQQQRSGGGNSQPQRGGGSGGAGTGRIRGNAFPTKFGNRNVFMKENSSSSSVDSRSRSRSSSRSPSRHFRRSDSDSDSSYSGNECRLGGRRNAQKNRGRGGHMDRGRMRTQRGKRHDQGPPKRNRKRNTMDYEDPEKEFKKERRAARFQHGAHPKKLRLEPLILQINALDPLGSDGLDWNELKIVGTCQDITKHYLRLTCAPDPSTVRPLSVLKKSLSAVKSHWKEKQDYAYACEQMKSIRQDLTVQGIRAEFTVEVYETHARIALEKGDHEEFNQCQTQLKSLYAENLPGNVGEFTAYRILYYIFTKNSGDITTELAYLTKELKTDPCVAHALALRAAWALSNYHRFFCLYRQAPCMSGYLIDKFAERERKTALKAMIKTFRPVLPVSYVVSELAFESEEECQAFLAALSLVYTSPDATKIDCKQSLAVLANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAZ0</accession>
    <name>A0A6I9YAZ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106548418</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921260.1">
        <property type="nucleotide sequence ID" value="XM_014065785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAZ0"/>
    <dbReference type="GeneID" id="106548418"/>
    <dbReference type="KEGG" id="tsr:106548418"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000024">
        <property type="entry name" value="Vomeronasal 2, receptor 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026662049" evidence="13">
        <location>
            <begin position="18"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="534"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="572"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="693"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="534"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013921260.1"/>
        </source>
    </evidence>
    <sequence length="751" mass="85493" checksum="AC41DDD9633492A9" modified="2020-10-07" version="1">MVLFAMLVILPLPQVVCKNHRAECKTSDPLPFLHKQYQSGDLIVVNMFSQFYVFQTVMDFSKRPSKELFEDTVIMTPIYQHILVLEFAITEINRDPLILRNRTLGFQIFNTYFMTSWTYQASLELFSTKGQFLTNYNCDTQHRPIAVLGGPTSDVCVHMATILSLYKMPQLIYGSTTEGNTKKETAIRQQMFPNIDGQYKGILLLLLHFGWTWIGILSAKDDSGENFVQNVVPMIAQKGICSAFLEIFPVIDYSNSIPELVEVGLQMYMVVMKSTANVVLVHGEIYGIIVLRLLSTNLKIEDAPLWRQGKIWVMTAEMDFTSLPFLRNSDLVFLHGALSFAIHTEKMLGFQQFLEMRNPTLEKEDTFIKLFWESAFECSFPTSVMEEESAIPCTGKEKLAALPTSVFEMDMTGHSYSIYNAIHVVAHALHDFHSSMMEYRARGHEVKQKHLDQKSWKVRPLSLCNSKCHSGYTRSKIEDKPFCCYNCRQCPHGKISNRDDMDDCFQCPEDQYANVNHDLCLPKIATFLMYEETLGITLASSALFFCLVTIGILWIFIKHQDTPIVKANNRNLTYVLLIALFLSFLCCFLFLGKPDKVTCLLRQTTFGVIFSVAISCVLAKTIIVILEFMATKPGSKMTRWVGNRLAVSILLCCTSLQGFICVIWLATSPPFPDADMSSGAEEIFLECNEGSVFMFYCVLSYMGFLAIISFTVAFLARKLPDVFNEAKFITFSMLVFCSVWLSFIPTYLQYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB09</accession>
    <name>A0A6I9YB09_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">LOW QUALITY PROTEIN: piwi-like protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PIWIL4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argonaute family. Piwi subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921300.1">
        <property type="nucleotide sequence ID" value="XM_014065825.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106548450"/>
    <dbReference type="KEGG" id="tsr:106548450"/>
    <dbReference type="CTD" id="143689"/>
    <dbReference type="OrthoDB" id="445936at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02845">
        <property type="entry name" value="PAZ_piwi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000014">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000003">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23278">
        <property type="entry name" value="Piwi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="10">
        <location>
            <begin position="193"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="11">
        <location>
            <begin position="467"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921300.1"/>
        </source>
    </evidence>
    <sequence length="773" mass="88770" checksum="E494E4BE561CEAEB" modified="2020-10-07" version="1">MERGGRIQCRFRDLEVNTREAMLHVKDSKSGTSGIPVKVVTNLFSLGLPREWQLYQYHVTFNPELESRRLRIALLYNHAEFLGKAKVFDGAILFLAHKLENKVTELSCETRRGDMVKITVQLTHQLFVSNPVCIQFLNLLFKKVVKXIPELHYSSTPLRKLTLWPGFAVSVGHFENKVMLCADVSHKVLRSESVLEFMTNLYNNSERARFAENCEKELVGLIVLTRYNNKTYRINDIEWSIKPTDTFQKRDGTQITYEDYYKQQYDVVLSDLNQPMLVSQLKSKGSRSDEPRVVHLIPELCYMTGLSKQATSDFRLMRDLAQHTHLPPQQRHQRLSRLADNIQRNKDARLELENWGLQMGCQASLTGRVISSEKILMQNESFSPVNSGDWFRDLRNMKIISETRLERWLVLCNYNHRDLTHNFLNCLRRVGSPMNFHIEYPRIVPIKEGPMSFVKVLQQHVNPELQLVLCVLSSNKKDCYDSVKKFLSLHQPIPSQCVLVRTLSKQGMMMSVATKIAMQIICKLGGELWAVEIPLKSLMVVGIDVNKDTLTKGNSVVGFVASLNPSITKWYSRCILQCAGTSMADCLRTCMEGAINNWQKCNGHPPARIIIYRDGVGDGQLKMVMDYEVPQLLSVLDESSGGCRPKMTLIIVKKKCLSRFFVESHGGLRNPPVGTVIDTEATQPTWYDFFLISQLARQGTVNPTYYNVVYDDNKLKPDHMQRLTFKMCHLYYNWPGLIRVPAPCQYANKLTFLVSQSIHREPSLELANKLFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBS1</accession>
    <name>A0A6I9YBS1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ST8SIA3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921567.1">
        <property type="nucleotide sequence ID" value="XM_014066092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBS1"/>
    <dbReference type="GeneID" id="106548665"/>
    <dbReference type="KEGG" id="tsr:106548665"/>
    <dbReference type="CTD" id="51046"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23970">
        <property type="entry name" value="GT29_ST8SIA3_oligo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000001">
        <property type="entry name" value="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF36">
        <property type="entry name" value="SIA-ALPHA-2,3-GAL-BETA-1,4-GLCNAC-R:ALPHA 2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="163"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921567.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="44090" checksum="ADA919434B1F0B6A" modified="2020-10-07" version="1">MRSCKMVRVVSVLGLVMVSVALLILSLISYVSLKKDNIFTTPKYANTGVPRMYMLHAGFRSQFALKFLDPSFVPNTNSLNQEVQDNKSPKWTFNRTAFLQQRREILQHVDVIKNFSLTKSMVRIGQLMHYDYSSHKYVFSISNSFRLLLPDVSPIQNKHYNICAVVGNSGILLGSQCGQEIDKYDFVLRCNFAPTEAFQKDVGRKTNITTFNPSILEKYYNNLLTIQDRNNFFLSLKKLDGAILWIPAFFFHTSATITRTLVDFFIEHRGQLKVQLAWPGNIMQHVNRYWKNKHLAPKRLSTGILMYTLASAICDEIHLYGFWPFGFNPNNREDLPYHYFDKKGTKFTTKWQESHQLPAEFQQLFRMHGEGLAKLTLSHCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCA0</accession>
    <name>A0A6I9YCA0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Regulator of G-protein signaling 3-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC106548829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921771.1">
        <property type="nucleotide sequence ID" value="XM_014066296.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013921772.1">
        <property type="nucleotide sequence ID" value="XM_014066297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCA0"/>
    <dbReference type="GeneID" id="106548829"/>
    <dbReference type="KEGG" id="tsr:106548829"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46848">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46848:SF1">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="62"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013921771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921772.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="13434" checksum="37B719A70C9BAC32" modified="2020-10-07" version="1">MELEVGVKGCIPEQFQDGNLSQVQASPKKLPAFLLKRRRKISQACVKERGQLKLSIHLQGRRLKLYVVEAKRLMGKQDRVCGSFVKVSIVPDTSRKCRQKSRTILGSTNPVFHEQFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCN6</accession>
    <name>A0A6I9YCN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Long-chain-fatty-acid--CoA ligase ACSBG1</fullName>
            <ecNumber evidence="16">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Acyl-CoA synthetase bubblegum family member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ACSBG1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the conversion of fatty acids such as long-chain and very long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="16"/>
    <dbReference type="RefSeq" id="XP_013921897.1">
        <property type="nucleotide sequence ID" value="XM_014066422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCN6"/>
    <dbReference type="GeneID" id="106548921"/>
    <dbReference type="KEGG" id="tsr:106548921"/>
    <dbReference type="CTD" id="23205"/>
    <dbReference type="OrthoDB" id="3633556at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF93">
        <property type="entry name" value="ACYL-COA SYNTHETASE BUBBLEGUM FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7 26" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0492">Microsome</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="24">
        <location>
            <begin position="42"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013921897.1"/>
        </source>
    </evidence>
    <sequence length="539" mass="60298" checksum="7D043FCBB76174A6" modified="2020-10-07" version="1">MSLEKDSALLWTTSADGQVKLRRDPTCSQAPITIHQMFLESLEKYKSLNALASKKEGKWEKITFSEYYRLSRKAAKSFLKLGLERFHSVAILGFNSTEWFILAVGAVFAGGIVSGIYTTSSPEACHYVADDSRANIVVVENQKQLDKIMQIWDRLPYLKAVVMYKDRVSEKHPNLYMMDEFMELGNEISDGRLDAIINSQEANQCCVLIYTSGTTGKPKGVMLSHDNITWTSAHASQAGDMQPAEIQQEKIVSYLPLSHIAGQMYDLWTGIKWGEEVYFAQPDALKGSLVEVLREALPTSQMGVPRVWEKMMEKIKEVSSQTSLLKRKMLSWAMSATLEQNLSCSNRDSKPFWMGLVDSLILAKIRWALGLSQCQKHFSGAAPLPVEVSHFFLGLNIPIYEGYGMSETSGPFCISGPHMYRLHSCGKVVPGCHVKIVNQDEDGNGEICFWGRNVFMGYLNMEEKTREVIDQDGWLHSGDLGKMDQDGFVYVTGRIKGGAFFAACFEALQMFGDHSFHTSSPPARVVGAFSLKHLEIALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YET6</accession>
    <name>A0A6I9YET6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Leucine-rich repeat neuronal protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LRRN4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922505.1">
        <property type="nucleotide sequence ID" value="XM_014067030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YET6"/>
    <dbReference type="GeneID" id="106549391"/>
    <dbReference type="KEGG" id="tsr:106549391"/>
    <dbReference type="CTD" id="164312"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF541">
        <property type="entry name" value="PROTEIN TOLL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026749222" evidence="11">
        <location>
            <begin position="18"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="713"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="608"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="474"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013922505.1"/>
        </source>
    </evidence>
    <sequence length="764" mass="85885" checksum="2827657208C9A5ED" modified="2020-10-07" version="1">MSNFLACVVVLVRIITARPTEKAGPALPEDANAPFLLIQQNVWEENINLTSLPCEELRNQISIAMQMQNQNLTSFPECLPELLEQLDLSVNLLPELNSQDVAYLPNLRILSLRQNQIRKVRWGTQNPSHLQFLDLSFNLLSVVPSCIISSLDNLRWLSLAGNPIREIQPFAFSCYPQLHFLNLSSTWLGKDGEMGIPESAFAMNLSHRPEYTEEARKALINILDLSATFLERIHEDWIKDLPQLTSFYLTQMPKLRRLDAAVFRNLPMLRELNCQDSHALSLVETESFHHIPRVTSLIFKNCNLSNFNPWNLTSSHRLSINLYGNPLVCHCAISWLLSNPDTVILQGASDTMCHFSPGDKETSSSMLLSKLYHQCQAETTTHSALSLVQSKLYHTLTTIDTEILADSNALPQKSLRDAFIPQSTSFTWGELTRKDPSKTGIVAYKEQAVHRSTEHPLTTPPLTTELSAILGNLSTEPPEVSTTEMKEREDTPTPHQSTLHSFTTEVTGTSPFKVSSALFISYSKTDSSENDLSLQDPTKSTSFANLASTNSLPDYTDDYDYENQQVASVAQTMDLCDYDPCRHLQKPCVDLQKLSSCLCPGMSDELTVPDPPRFHEISEMRDTSAQIHWCSPNSAVRFYQLAYRPKGSKKNYTNSGEIYATARRYTLYNLLPGSTYQVCIIAWNKAGSSQTTGWNEHNPPCSTFVTKSSYTSIFAALCTTSGVFLIATILLSVCLCKKYKTPHIEQYNTHLVSYKNPAFDYSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEW1</accession>
    <name>A0A6I9YEW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bardet-Biedl syndrome 7 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BBS7</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of BBSome complex.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922706.1">
        <property type="nucleotide sequence ID" value="XM_014067231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEW1"/>
    <dbReference type="GeneID" id="106549541"/>
    <dbReference type="KEGG" id="tsr:106549541"/>
    <dbReference type="CTD" id="55212"/>
    <dbReference type="OrthoDB" id="414590at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016575">
        <property type="entry name" value="Bardet-Biedl_syndrome_7_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056334">
        <property type="entry name" value="BBS7_GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056335">
        <property type="entry name" value="BBS7_hairpin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056333">
        <property type="entry name" value="BBS7_pf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056332">
        <property type="entry name" value="Beta-prop_BBS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16074">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16074:SF4">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23360">
        <property type="entry name" value="BBS7_GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23349">
        <property type="entry name" value="BBS7_hp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23361">
        <property type="entry name" value="BBS7_pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23743">
        <property type="entry name" value="Beta-prop_BBS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011091">
        <property type="entry name" value="BBS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0966">Cell projection</keyword>
    <keyword evidence="1" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="BBS7 beta-propeller" evidence="7">
        <location>
            <begin position="21"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="BBS7 GAE" evidence="5">
        <location>
            <begin position="376"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="BBS7 platform" evidence="6">
        <location>
            <begin position="493"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="BBS7 helical hairpin" evidence="4">
        <location>
            <begin position="598"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013922706.1"/>
        </source>
    </evidence>
    <sequence length="728" mass="81893" checksum="C15F4664E4086851" modified="2020-10-07" version="1">MELSLNRMDYLQVGVTSQKTMKLLPASGRKATQKVVTGDHDGVITCFGIKKGEAIPVFKTLPGEKIARLELGGALNTLQEKIFVAAGSEVRGFTKKGKQFLSFNTNLTENIKAMYISGSDLFLGASYIYNHYCDCKDQHYYLSGDKINDVLCLPPDKLSCIIPVLACQDRVLRVLQGSELLYEMEVPGPPAVLALNNGNGGDTGEDVLFGTSDGKIGLVQITGVSPIEKWKVGNEKKRGGILCMDSFDILGDGVKELLIGRDDGILEIYKFETEKSPVFKYEYALSESVTSIQGGCVGKEGYDEIVTSTFSGWVSGLTTEPIHGECGLDELKMTHEMQTKVSFLRNELEQLQMKVLQEREKYQQSSQSSTAISAVPTFSINDKFALNKDDASYSLVLEVQAAIDIVLLQCDVPIDLKDVDKNSAVVSFTDCEAEPNGNFLLATYRCQVHTTRIELKILSIEGRYGTLQAYVIPRVQPKTCQVRQYQIKPLSLHQRTHCLDHDRPMNTLTLKGQFSFAEVHSWIAFCLPEVPEKTPVGDSVSFYFQNTFLDTQLECTYRKGEGYFKSDNISTISILKDVLSKEATKRKINLNISCDMDEASVNHTLNLIHPKLEYQLMLAKKVELIDALKELQAHEGNMDFLIPEYRNILEESDQLLIEHKKQPTSLERLYGWLINFFLLLLLVLAVMGKLWENKCVILVEYLFPFDKRCSFKNEKHTMQCFMLKEGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF39</accession>
    <name>A0A6I9YF39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lamin-B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106549612</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus lamina</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922787.1">
        <property type="nucleotide sequence ID" value="XM_014067312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF39"/>
    <dbReference type="GeneID" id="106549612"/>
    <dbReference type="KEGG" id="tsr:106549612"/>
    <dbReference type="OrthoDB" id="102442at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005652">
        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090435">
        <property type="term" value="P:protein localization to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000007">
        <property type="entry name" value="Lamin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1260:FF:000002">
        <property type="entry name" value="Lamin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721">
        <property type="entry name" value="LAMIN DM0-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721:SF3">
        <property type="entry name" value="LAMIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="13">
        <location>
            <begin position="34"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="12">
        <location>
            <begin position="318"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="443"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="31"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="151"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="443"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013922787.1"/>
        </source>
    </evidence>
    <sequence length="477" mass="53736" checksum="22AB214F8AE81388" modified="2020-10-07" version="1">MAAAVAASTPIGQRTRSSAVSTPLSPTRITRLQEKEELQDLNDRLAIYIDKVRSLEIENSALHLQVTEREEVRGRELTGLKSLYENELADARRSLDDTARDRAKLQIELGKIRAEHDQLLGNYAKKESDLNCALAKLREFEASLNTKEAALATALGDKKSLEGELEDLKDQFNQLEAAFAAAKKQLVDETLLKVDLENRCQSLIEDLEFRKNMYEEEINETRRKHETRLVEVDSGRLIDYEYKLTQALKEMREQHDMQVKLYKEELEETYHAKVLLKLTPSPSSRVTVSRASSSRSVRTTRAKRKRVDVEESEASSSVSIAHSASATGNICIEEIDVDGKFIRLKNTSDQDQPMGGWEMIRKIGDVSVNYKYTSRYVLKAGQTVTIWAANAGITASPPSDLIWKNQNSWGTGKDVKVVLKNSQGEEVAQRSTVFKTTLLEVEEGEEVEDEPTEAVEEEDLFHQQGDPRGANRSCAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH79</accession>
    <name>A0A6I9YH79_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CAP-Gly domain-containing linker protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CLIP3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923537.1">
        <property type="nucleotide sequence ID" value="XM_014068062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH79"/>
    <dbReference type="GeneID" id="106550201"/>
    <dbReference type="KEGG" id="tsr:106550201"/>
    <dbReference type="CTD" id="25999"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000044">
        <property type="entry name" value="CAP-Gly domain containing linker protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000005">
        <property type="entry name" value="CAP-Gly domain containing linker protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF77">
        <property type="entry name" value="CAP-GLY DOMAIN-CONTAINING LINKER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="187"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="5">
        <location>
            <begin position="304"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="354"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013923537.1"/>
        </source>
    </evidence>
    <sequence length="419" mass="45799" checksum="BAA6F0EF16232FD7" modified="2020-10-07" version="1">MTKDDSAELPSEEDHQALEFQSPTLERRKKPIVHPSAPAPLPKDYAFTFFDPNDPACQEILYDPITTVPELFAIIRQWVPQVQHKIDMIGNEILKRGCHVDDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLSNQLLALEADVTLRSRWTNMNALHYAAYFDVPELIRILLKASKPKVLNSTCNDFNHGTALHIAASNLCLGAVKCLLEHGANPAVRNSKGQVPADVVPDPMDMTLDKAEAAMIAKELKQLLLDAVPLSCSLPKVTLPNYDNIPGNLMLLSLGLKLGDRVLVDGQKVGTLRFCGTTEFASGQWVGVELDKPDGKNDGSVGGIRYFICPPKHGLFASVSKISKAADQTPSVTSTPRTPRMDFSRMTAKGRKEKDKDRKGALLRGGCRSTPHTHFPPGSWQSPENGFFPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHA9</accession>
    <name>A0A6I9YHA9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein O-mannose kinase</fullName>
            <ecNumber evidence="2">2.7.1.183</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein kinase-like protein SgK196</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sugen kinase 196</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20 21" type="primary">POMK</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-O-[beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-Man]-L-Thr-[protein] + ATP = 3-O-[beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52616"/>
            <dbReference type="Rhea" id="RHEA-COMP:13308"/>
            <dbReference type="Rhea" id="RHEA-COMP:13309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136709"/>
            <dbReference type="ChEBI" id="CHEBI:136710"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.183"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.183" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013923567.1">
        <property type="nucleotide sequence ID" value="XM_014068092.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923575.1">
        <property type="nucleotide sequence ID" value="XM_014068100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHA9"/>
    <dbReference type="GeneID" id="106550224"/>
    <dbReference type="KEGG" id="tsr:106550224"/>
    <dbReference type="CTD" id="84197"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019200">
        <property type="term" value="F:carbohydrate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000464">
        <property type="entry name" value="Protein O-mannose kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039318">
        <property type="entry name" value="POMK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22618">
        <property type="entry name" value="PROTEIN O-MANNOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22618:SF2">
        <property type="entry name" value="PROTEIN O-MANNOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 20 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="89"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013923567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013923575.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="41288" checksum="37A40BF8A1C0D852" modified="2020-10-07" version="1">MKDDSFTTWTKKACDLKSDIKQKLILLALLLFLTVLLVILFLYQYLGNVYFSYGESYVEQNFCPRGYFKLGTMKNCSPWLSCQAIKREVRKLKHVGGGAVKRVFLSEWKENKVALSQLTVPELQEDFIHGLQMLKKLQSKHVVKLLGYCEEEFMILTEYHPFGSLKNVNEILNLPKYKNYNTWHNRFLLAIGYVSIIHFLHNNPLGTFVMCDSSDLNKTLSQYLLTSSFQVIVNDLDALPLLNRRAGELIKCGHRELEGEWVAPEQLWPHGKEVPFEDHLMPAYNEMIDIWKIPDVSNFLLGDVDGSDLVRFHLFSIHEACKKNAAERPSSQMVLDRYNNVLASLVKEATVSNTRDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHB6</accession>
    <name>A0A6I9YHB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrator complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">INTS10</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Component of the Integrator complex, composed of core subunits INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, INTS11/CPSF3L, INTS12, INTS13, INTS14 and INTS15. The core complex associates with protein phosphatase 2A subunits PPP2CA and PPP2R1A, to form the Integrator-PP2A (INTAC) complex. INTS10 is part of the tail subcomplex, composed of INTS10, INTS13, INTS14 and INTS15.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Integrator subunit 10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923586.1">
        <property type="nucleotide sequence ID" value="XM_014068111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHB6"/>
    <dbReference type="GeneID" id="106550240"/>
    <dbReference type="KEGG" id="tsr:106550240"/>
    <dbReference type="CTD" id="55174"/>
    <dbReference type="OrthoDB" id="18145at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026164">
        <property type="entry name" value="Int_cplx_su10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16055">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16055:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21045">
        <property type="entry name" value="INT10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02106">
        <property type="entry name" value="INTSUBUNIT10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="365"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013923586.1"/>
        </source>
    </evidence>
    <sequence length="736" mass="84822" checksum="8D5BF602D5B9B8A3" modified="2020-10-07" version="1">MSALGDCEFLVKRARELVQGDLWAAKAWLITARSLYPADFNIQYEMYSIEKNAERTASAGRLLYDMFVNFPEQPVVWREISIITSALRNDSQDKQTEFLKGLFETLPGRVQCEMLLKATDQCFNTLERAEMLLLLLRRFPETVVQHGVGLGETLLEAENIEDQESPVNCFRKLFVCDVLPLIINNPDVRLPSTLLYKYLNKAAEFYINYVTRSSPTENQYPGTQDTSDLVSPNKRSAQKYIIEGLTEKSCQITDPWERIFKILSIVGVRCEWQMDVARRSFGDILLKMKDVCRYISNYDNEAHAKYKTQVVYSTMLVFFKNAFQYISSIQPSLFQGSNASNQVPLILIEDVSNVFGDTDIERSKHVHKKRKLPEGREKTMSSDDEDPSGKGKNRVITINATDLPNSVEVQESFRLARESWELLHSLESLDKEFTRICVSWKTDTWLWLRIFLTDMIIYQGQYKKAIASLHHLAALQGSHCPQQIMGQGSLENQRPLIQLATCHFALGEYHLTCEKILDLMCFMTLPIQEGAKIQEDQPVLKSKFRKVSELKLWPCNSKTIMPYCLRLLLACFKLKAFTDNRDDMALGHVIVLLQHEWPRGENLFLKAVNKICQQANFQYEKFFNYITNIDMLEEFAYLRTQEGGKIHLELLPNQAMLIKTSSPPLGLLQQEFIPVLQPSMQTADRHHTITRGITKGVKEDFRLAMERQVSRCGENLMMVLHRFCMSEKILLLQTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHT8</accession>
    <name>A0A6I9YHT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Copper-transporting ATPase 1</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Copper pump 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ATP7A</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="20">Monomer. Interacts with PDZD11. Interacts with ATOX1 and COMMD1. Interacts with TYRP1. Directly interacts with SOD3; this interaction is copper-dependent and is required for SOD3 activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013923534.1">
        <property type="nucleotide sequence ID" value="XM_014068059.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550199"/>
    <dbReference type="CTD" id="538"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000026">
        <property type="entry name" value="ATPase copper transporting alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000009">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000031">
        <property type="entry name" value="copper-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000092">
        <property type="entry name" value="copper-transporting ATPase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000144">
        <property type="entry name" value="copper-transporting ATPase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000023">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594:SF4">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0187">Copper transport</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="720"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="763"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="789"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="955"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="999"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1377"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1408"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="14"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="177"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="284"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="389"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="506"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="582"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00062031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00074950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00083612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013923534.1"/>
        </source>
    </evidence>
    <sequence length="1518" mass="164802" checksum="04D3312D0F51F350" modified="2020-10-07" version="1">MQEDKRMDPSSGARSIVVQVEGMTCSSCVQTIEQHIGELNGVHVIKVSLDEKSATIVYNPKEQTPQSLQEAIEDMGFDAVLGNTNSQSVPHDTTFLMIPTQAALTCEQIRTDLLKIKGILDVKISSDKTTLVVTFISSILNSKQINQIVPGLNSDNALQDENQGTFEDVSLNQINDVVLRLKIEGMTCHSCTSTIEGKIGKLQGVKRIKVSLDNQEAVVSYQPHLITSEEIKKQIEAAGFIASVKKMPKPLQLGSIDIERLKSSQTKRSERTPRESSEGELSMKTVAFHVDGMHCNSCVFNIQNSLLALPSVSRITVSLQEKSAVVQYNPTLISLSALRRAIEAVSPGTFKVCVLDAHEKAEHLNNSKSPSKSSLPAAVQNIGPHPLMLVTVIRIGGMTCHSCVQSIEGLISQKPGVKSVCVSLRDRNGTIEYDPTLTSPADLKNSIEDMGFDASIAGPPSPDPMVLITQPSQERLARTQESKLPKTVAHANLSPAPNRLGLKAPSKCYIQITGMTCASCVANIERNLKREDGICSVLVALMAGKAEVRYDPSVIQPPTIAERIRELGFGATVLEDYDEGDGVLELIVRGMTCASCVHKIESTLMKTKGVLYSSVALATNKAHVKYDLEIIGPRDIIQIIQNLGFNATLVKKDRSASHLDHKQEIRQWKRSFLVSLLFCIPVMGLMIYMMVMDSHMSAASNMTQEDIEDNHPSMFLEQQILPGLSIMNLLSFLLCVPVQFFGGWHFYIQAYKALKHKTANMDVLIVLATTIAFVYSFVVLLVAMAEQAVVNPVTFFDTPPMLFVFIALGRWLEHVAKGKTSEALAKLISLQATEATIVTLGPDNILVSEEQVDVELVQRGDIVKVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVMKKPGSSVIAGSINQNGSLLISATHVGSDTTLCQIVKLVEEAQTSKAPIQQFADKLSGYFVPFIVGVSVITLFAWIVIGFVNFQLVETYFLGYNKSISKAEVVIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVMFDKTGTITHGTPVVMQMKMLVESNRIPRMKVLALMGTAESHSEHPLGAAITKYCKQELDADTLGTCTDFQVVPGCGISCKVTNIEALLRSKSETVEETNVRNAALVNMDEKTDPLMQPALIINEQVPVFPSDTWMLHKYSVLIGNREWMSRNGLLLKSDIDETMMEHERRGRTAVLIAVDGELCGLVAIADTVKPEADLAVRTLKGMGLEVVLITGDNGKTARSIASQVGITKVFAEVLPSHKVAKVRQLQEEGKRVAMVGDGINDSPALAMADVGIAIGTGTDVAIEAADVVLIRNDLLDVVASIDLSRKTVKRIRINFIFALIYNLIGIPVAAGVFLPIGLVLQPWMGSAAMAASSVSVVLSSLLLKLYKKPTCEKYERHARTQVRCKSTSEISVHIGIDDVGPGSPKLSLMDRIIHYSQASISSLFSDKHSLNNMALSEPDKHSLLVGDSQEEDDTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIE5</accession>
    <name>A0A6I9YIE5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sister chromatid cohesion protein PDS5 homolog B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PDS5B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923600.1">
        <property type="nucleotide sequence ID" value="XM_014068125.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550255"/>
    <dbReference type="KEGG" id="tsr:106550255"/>
    <dbReference type="CTD" id="23047"/>
    <dbReference type="OrthoDB" id="200660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001146">
        <property type="entry name" value="PDS5 cohesin associated factor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000064">
        <property type="entry name" value="Sister chromatid cohesion protein PDS5 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF1">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN PDS5 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1137"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1139"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1156"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1172"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1196"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1262"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1307"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1322"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1350"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1363"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1443"/>
            <end position="1454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013923600.1"/>
        </source>
    </evidence>
    <sequence length="1454" mass="165997" checksum="25569359E373BFDA" modified="2020-10-07" version="1">MAHSKTRANDGKITYPPGVKEISDKISKEEMVRRLKMVVKTFMDMDQDSEEEKELYLNLALHLASDFFLKHPDKDVRLLVACCLADIFRIYAPEAPYTSPDKLKDIFMFITRQLKGLEDTKSPQFNRYFYLLENIAWVKSYNICFELEDSNEIFTQLYRTLFSVINNGHNQKVHMHMVDLMSSIICEGDTVSQELLDTVLVNLVPAHKNLNKQAYDLAKALLKRTAQAIEPYITNFFNQVLMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNEERLQVVKLLAKMFGAKDSELAAQNKPLWQCYLGRFNDIHVPIRLECVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDLLLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYSLQSEAGKEAAKQIAWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETNERMKCLYYLYATLDSNAVKALNEMWKCQNLLRHQVKDLVDLIKQTKSDASSKAIFSKVMVITRNLPDPGKAQDFMKKFTQVLEDDDKIKHQLEMLVSPTCSCKQAEGCVREITKKLGNPKQPTNPFLEMVKFLLERIAPVHIDTESISALIKQVNKSIDGTADDEDEGVPTDLAIRAGLELLKVLSFTHPISFHSAETFESLLACLKMEDEKVAEAALQIFKNTGSRIEENFLHIRSALLPVLQHKAKKGPPRQAKYAIHCIHAIFSSKETQFAQIFEPLHKSLDPSNLEHLITPLVSIGHIALLAPDQFAAPLKSLVATFIVKDLLMSDRMPGKKTTKLWVPDEEVSPETLVKMQAIKMMVRWLLGMKNNHSKSGTSTLRLLTTILHSDGDLTEQGKISKPDMSRLRLAAASAVVKLAQEPCYHEIITLEQYQLCALAINDECYQVRQAFAQKLHKGLSRLRLPLEYMAICALCAKDPVKERRAHARQCLVKNINVRREYLKQHAAVSEKLLSLLPEYVVPYTIHLLAHDPDYVKIQDIEQLKDIKECLWFILEILMAKNENNSHAFIRKMVENIKQTKDAQGPEDTKMNEKLYTVCDIAMNIIMSKSTTYSLESPKDPVLPARYFTQPDKNFSNTKNYLPPEMKSFFTPGKPKAANVLGAVNKPLSSAGKQSQSKSSRMETVSNASSNSNPSSPGRIKGRLDSTELDHSENEDYTMTSSLPGKKNDKRDDSEISELEKPRSRKKMITDPEEKLGMDELNKLGQESKLRSSQRGRKRAAVISESEEPQWSEEKRLKDSILESEDEQNSPPKKGRRGRPPKAATGTIQKEESTVKTSKRGRKKAAPVKPMEEEEEEEEEEEEEEEEEERQIDSIEPKSKGRQYKAPKKSQQRSESSEISTAESNQSTPQKRRGRPPKVPPTQQKKGGAGRSKQTASKENESSEEMDVFQSSSPIHDIPQEVTMEEEEVSSIRVRRRTSKRERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIU3</accession>
    <name>A0A6I9YIU3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Bile salt export pump</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33 34" type="primary">LOC106550719</name>
    </gene>
    <organism evidence="32 34">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner. Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts. Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>cholate(in) + ATP + H2O = cholate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50048"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29747"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:50049"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>glycochenodeoxycholate(in) + ATP + H2O = glycochenodeoxycholate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36252"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:50061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>glycocholate(in) + ATP + H2O = glycocholate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29746"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:50057"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>glycoursodeoxycholate(in) + ATP + H2O = glycoursodeoxycholate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50068"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:132030"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:50069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>pravastatin(in) + ATP + H2O = pravastatin(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63908"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:63660"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:63909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>taurochenodeoxycholate(in) + ATP + H2O = taurochenodeoxycholate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50064"/>
            <dbReference type="ChEBI" id="CHEBI:9407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:50065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>taurocholate(in) + ATP + H2O = taurocholate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50052"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36257"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:50053"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>tauroursodeoxycholate(in) + ATP + H2O = tauroursodeoxycholate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50072"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:132028"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:50073"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="28">Interacts with HAX1. Interacts with the adapter protein complex 2 (AP-2) throught AP2A2 or AP2A1; this interaction regulates cell membrane expression of ABCB11 through its internalization in a clathrin-dependent manner and its subsequent degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924146.1">
        <property type="nucleotide sequence ID" value="XM_014068671.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924147.1">
        <property type="nucleotide sequence ID" value="XM_014068672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIU3"/>
    <dbReference type="GeneID" id="106550719"/>
    <dbReference type="KEGG" id="tsr:106550719"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015125">
        <property type="term" value="F:bile acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015722">
        <property type="term" value="P:canalicular bile acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000018">
        <property type="entry name" value="ATP-binding cassette subfamily B member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000479">
        <property type="entry name" value="Multidrug resistance protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF23">
        <property type="entry name" value="ATP-BINDING CASSETTE SUBFAMILY B MEMBER 11, GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="31">
        <location>
            <begin position="5"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="30">
        <location>
            <begin position="361"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_013924146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_013924147.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="62258" checksum="42272FD2CF40B9B9" modified="2020-10-07" version="1">MMLSGGICAILHGIAQPAMLIVFGIMTDTFIQYDIEKQELNDPNKVCMNNTIVWINSSHHQNVSNASMSCGLLDIESQMIYFASSYAGTGFAVLVLGYLQICLWVMSAAHQTQQIRKAYFRKIMRMDIGWFDCTSVGELNTRISDDVNKINDAIADQVAIFIQRFTTFVCGFLLGFVRGWKLTLVIIAVSPLIGIGAGIIGLSVAKLTGLELKAYAKAGAIADEVLSSIRTVSAFGGEKKEVERYNKNLVFAQNWGIRKGIIMGFFTGYIWCIIFLCYALAFWYGSKLVLEDKEYSAGTLLQVFLGVLIGALNLGQAAPCLEAFATGRGAATNIFETIDEKPKIDCMSEDGYKLDKVRGEIEFHNVTFHYPSRPDVKILDNLSMVIKPGETTAFVGPSGAGKSTTIQLIQRFYDPTEGMVTLDGHDIRSLNIQWLRSLMGVVEQEPVLFSTTIAENISYGREDATMEDIVRAAKEANAYNFIMDLPLQFDTLVGEGGGQMSGGQKQRIAIARALVRNPKILLLDMATSALDNESEATVQKTLSKVGNVILCIYVIFIFYFLSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIV0</accession>
    <name>A0A6I9YIV0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106550480</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923879.1">
        <property type="nucleotide sequence ID" value="XM_014068404.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550480"/>
    <dbReference type="KEGG" id="tsr:106550480"/>
    <dbReference type="OrthoDB" id="1508846at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017385">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF5">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT E 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038097">
        <property type="entry name" value="V-ATP_synth_e1/e2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923879.1"/>
        </source>
    </evidence>
    <sequence length="81" mass="8908" checksum="869374DE2913AE1B" modified="2020-10-07" version="1">MAYGGLLVPIIVMSVFWGVIGGVLPWLVPKGPNQGVINTMLVITAVCCYLFWLIAILAQLNPLXGPQLKSDTILYLKEQWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ65</accession>
    <name>A0A6I9YJ65_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calglandulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106550935</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the cellular control mechanism of the secretion of toxins from the gland into the venom.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calmodulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924398.1">
        <property type="nucleotide sequence ID" value="XM_014068923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ65"/>
    <dbReference type="GeneID" id="106550935"/>
    <dbReference type="KEGG" id="tsr:106550935"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924398.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16943" checksum="080E0600F655631F" modified="2020-10-07" version="1">MTSLTEQEIAEYKEAFMLFDRNGDGTITTRELGTVLRSLGQNPTEAELREIVHKLETDKNGTIDFPDFLNLMEKEIKNRDCHEIRKAFQVFDKDGNGYISAAELRHIMTNLGEKLSKEEVEEMLKVADLDGDGQMNYEEFLRMMTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJX1</accession>
    <name>A0A6I9YJX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metal transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CNNM2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Metal transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ACDP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924065.1">
        <property type="nucleotide sequence ID" value="XM_014068590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJX1"/>
    <dbReference type="GeneID" id="106550653"/>
    <dbReference type="KEGG" id="tsr:106550653"/>
    <dbReference type="CTD" id="54805"/>
    <dbReference type="OrthoDB" id="5353557at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000001">
        <property type="entry name" value="Putative metal transporter CNNM3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057492">
        <property type="entry name" value="Ig_CNNM1/2/4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF22">
        <property type="entry name" value="METAL TRANSPORTER CNNM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25562">
        <property type="entry name" value="CNBH_CNNM2_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25511">
        <property type="entry name" value="Ig_CNNM4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026933678" description="Metal transporter" evidence="15">
        <location>
            <begin position="48"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="17">
        <location>
            <begin position="257"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="524"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013924065.1"/>
        </source>
    </evidence>
    <sequence length="848" mass="93809" checksum="A2C0A61B054968F1" modified="2020-10-07" version="1">MAAPLPAVAPRLMAGPAGGGERETRMLPRSPLLLPLLLGILLSRCLGEQPARAAAASSSSSSSSSLLPGASDSEETVIIGLRLEDTDDVSFMEKGVLRVSERTRVKLRVYGQNINNETWSRIAFTEHERWRDGEGRRPPAEQEDGPVPVGSLSSAPQRCGIRTSDIIILPHITLNRRTSGIIEIDIKPLRKTEKSKSYYLCTSVSSPAILGGAGSVAGSPGVGAGIIGPDGGPWTETTWIYHDGEDTKMIVGEEKKFLLPFWLQVIFISLLLCLSGMFSGLNLGLMALDPMELRIVQNCGTEKEKNYAKRIEPVRRQGNYLLCSLLLGNVLVNTTLTILLDDIAGSGLVAVVVSTIGIVIFGEIVPQAICSRHGLAVGANTIFLTKFFMMMTFPASYPVSKLLDCVLGQEIGTVYNREKLLEMLRVTDPYNDLVKEELNIIQGALELRTKTVEDVMTPLRDCFMITAEAVLDFNTMSEIMESGYTRIPVFEGDRSNIVDLLFVKDLAFVDPDDCTPLKTITRFYNHPLHFVFNDTKLDAMLEEFKKGKSHLAIVQRVNNEGEGDPFYEVLGIVTLEDVIEEIIKSEILDETDLYTDNKTKKKVAHRERKQDFSAFKQTDSEMKVKISPQLLLAMHRFLATEVEAFGPSQMSEKILLRLLKHPNVIQELKYDEKNKKAPDYYLYQRNKPIDYFILILQEQHIAYKEKSICPVSTIAPRKRCNPSGPGDLNSLKGKVEVEAGKEGMKFEAGAFSYYGVMALTASPVPLSLSRTFVVSRTDILAPGSPAENKSPPRSCGLNHSDSLNRGDRIDAMTPAIGNSNNQLNSSFLQVYVPDYSVRAISDIQYVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJZ4</accession>
    <name>A0A6I9YJZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Tensin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106550874</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924334.1">
        <property type="nucleotide sequence ID" value="XM_014068859.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550874"/>
    <dbReference type="KEGG" id="tsr:106550874"/>
    <dbReference type="OrthoDB" id="6273691at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010761">
        <property type="term" value="P:fibroblast migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01213">
        <property type="entry name" value="PTB_tensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09927">
        <property type="entry name" value="SH2_Tensin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000002">
        <property type="entry name" value="Tensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1110:FF:000002">
        <property type="entry name" value="tensin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000010">
        <property type="entry name" value="tensin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035012">
        <property type="entry name" value="Tensin-like_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033929">
        <property type="entry name" value="Tensin_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF3">
        <property type="entry name" value="TENSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="11">
        <location>
            <begin position="49"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase tensin-type" evidence="12">
        <location>
            <begin position="140"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="13">
        <location>
            <begin position="317"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="10">
        <location>
            <begin position="1622"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="12"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1022"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1163"/>
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            <begin position="1435"/>
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        <location>
            <begin position="1521"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="535"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="1571"/>
            <end position="1586"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013924334.1"/>
        </source>
    </evidence>
    <sequence length="1847" mass="199690" checksum="FB5ACEB1A0EC5729" modified="2020-10-07" version="1">MGCTVNLGCCEGNPRPPQSLVPPRQTPKQPGKRKRKQLPRPQDLEASTSHTFKVKSFKKVKPCGICRQAITREGSTCRGCKLSCHKKCEAKAATPCMPVGNHELPSTEESPAKHVDTPVFSRSPQIMQAQRKPSRNLSLNQAMEDTCELDLVYVTERIIAVNFASLSEEQTFCSNLKEVAQMLKSKHGDSYLLLNLSERRHDISKLHSKVLDFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRTGVVIAAYMHYSNISASADQALDRFAMKRFYEDKVIPAGQPSQKRYVYYFSGLLSGTIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKEFRSPTRDVIFRVQFHTCAIHDLAIVFNKEDLDDAFKDERFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRRDSYENFNIRREDSMDEVGHTQGPLDGSLYAKVKKKDSLHGSTGTVNPNRLALSAAPNHVEHTLSVSSDSGNSTASTKTDKTDEQAAPSVPSSSGGNNPILTPEEKRELESLLSGFGLENEATMHNHRPSEIAAAVSASPGRHIIPAQIHVNGEAAGLVAERETDILDDELPNQDGHSVGSLGTLSSFDGTTNTSDIGYHEAPRMESLNSLPNGSSNFNGLDKLHDSEMVINGGYPYNNQNALRNIMGRHPHDPYAHIRPSVSAQENLADYHQHGFPTPGWLQPQPPASSQPYPYGCDSNSIYRSQSFPTATVEISQLPQAPARSTSSREAVQRGLNSWQQQGGGSRPPSHQQDGARDSHSSSLASASPQSSPSQAAPSQSISIPEFPRVASQKEIEQSIEALNMLMHDLDPSVSMMPKSQSVPLATREDRPHPVALSMSAQPVIGPHVVQPKATNAFGLIPGSPSQNSLGSSVPQMAEPIPKAHPMKLLLNSVGEPEYPTQDFREAYSPYGYQNQQPTVIQGKSLGQMAGSPPTSPRPHATACKVLEGQHPLLTSPPSSTTAVQPQMPLNEAEHQGEASQASGEEPLHLEGLVAHRVAGYLALLNPPSSVPTGVQLPQEKSPDEGEALVRRRTTSEGQYENSPQEPSSPRSPTVRSPIHCFSPEVVSSIAANPGGRPKEPHLHSYKEALEEIQGTSPSSPPSSGGETSPQTPAFPVSPQTPYSNLLRSPPGLAKTPLSALGLKPHNPAEIFLQQTGEVYGETVVEYDQEPRSYVESVARTAAATGNSSGSGISLAGKFQPSLALETYSTETSGRNESLNNSFTLPSPASASSPIHSMDGISIGSYPSENSASIAISFQPSAESSFQSTNLSQGPSAHSSYQNTSTSTQGLPVSINADFPDGRTSAQQDNHPTPQAQIMASGVQVMPGSPHALHRTVATNTPPSPGFGRRTVSTNMVVIPGSPSMNRHLMGSHSTVVSSPGSPSHARHQPLAGGSTAMISRSPSLDRHLVCGYSTPEEKQQTLSRQSSASGYQAPSTPLFPVSPAYYHGISPHSSSPDSAVYRQGSPTPPQPLLPEKKRMSSGDRSNSLPNYATVNGKTSSPISSGMSSPSSGSSMAFHHTLPDFSKFSMPDSSPETRANVKFVQDTSKYWYKPEISREQAIALLKDKEPGAFIIRDSHSFRGAYGLAMKVASPPPGVIQQSKKGDITNELVRHFLIETSPKGVKLKGCPNEPNFGCLSALVYQHSMMPLALPCKLVIPDRDPMDETKERASSSSSATDLLKQGAACNVLFINSVEMESLTGPQAIAKAITETLAADIPPSATIVHFKVSTQGITLTDNQRKLFFRRHYPIVTVTFCDVDPQDRKWTKSESGGAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLL1</accession>
    <name>A0A6I9YLL1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-galactosidase</fullName>
            <ecNumber evidence="15">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106551524</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a globoside Gb3Cer (d18:1(4E)) + H2O = a beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:21112"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17950"/>
            <dbReference type="ChEBI" id="CHEBI:18313"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:21113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a globoside Gb3Cer + H2O = a beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1)-ceramide + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:48020"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:79208"/>
            <dbReference type="ChEBI" id="CHEBI:88154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:48021"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013925122.1">
        <property type="nucleotide sequence ID" value="XM_014069647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLL1"/>
    <dbReference type="GeneID" id="106551524"/>
    <dbReference type="KEGG" id="tsr:106551524"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046479">
        <property type="term" value="P:glycosphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000070">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000017">
        <property type="entry name" value="Alpha-galactosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035373">
        <property type="entry name" value="Melibiase/NAGA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF93">
        <property type="entry name" value="ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17450">
        <property type="entry name" value="Melibiase_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027073011" description="Alpha-galactosidase" evidence="16">
        <location>
            <begin position="22"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase A C-terminal" evidence="17">
        <location>
            <begin position="327"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013925122.1"/>
        </source>
    </evidence>
    <sequence length="421" mass="46744" checksum="59A887813CCAC39F" modified="2020-10-07" version="1">MPDRRALLWLPVQPLLPLSLGVLLGLGPAVSPLDNGLLRTPPMGWEPWLRFKCNTDCEKDPENCISEHLVKAMADRLAADGWKELGYEYVTLDDCWAAGKRDARGRLQADPQRFPSGMKALADYVHSKGLKFGIYSDLGNATCAGYPGTTLDTVETDANTFAEWGVDMLKLDGCYSDSSLKAIGYPKMSRALNQTGRPIALSCSWPAYEGGLPPKVNYTLLGQICNFWRNYADIEDTWESLFHIIEWYGDHQDILQPASGPGRWNDPDMLIVSDLGLSHEQSKVQAALWAILAAPFFISCNLRNISKEAKEMLQNPLLIYINQDLRGVQGRRIARKQNLEVWKRPLVNGQYAVAVLNNRTDGAPQLFATTPSLLGISDCRAGYRLYDVLGRLSMGVYAFNDTISAKVTPTGVLLLFLNPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YM66</accession>
    <name>A0A6I9YM66_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Agouti-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">AGRP</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925322.1">
        <property type="nucleotide sequence ID" value="XM_014069847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YM66"/>
    <dbReference type="GeneID" id="106551702"/>
    <dbReference type="KEGG" id="tsr:106551702"/>
    <dbReference type="CTD" id="181"/>
    <dbReference type="OrthoDB" id="9942042at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070996">
        <property type="term" value="F:type 1 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008343">
        <property type="term" value="P:adult feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000253">
        <property type="term" value="P:positive regulation of feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.760.10">
        <property type="entry name" value="Agouti domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007733">
        <property type="entry name" value="Agouti"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027300">
        <property type="entry name" value="Agouti_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036836">
        <property type="entry name" value="Agouti_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16551">
        <property type="entry name" value="AGOUTI RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16551:SF4">
        <property type="entry name" value="AGOUTI-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05039">
        <property type="entry name" value="Agouti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00792">
        <property type="entry name" value="Agouti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57055">
        <property type="entry name" value="Agouti-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS60024">
        <property type="entry name" value="AGOUTI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51150">
        <property type="entry name" value="AGOUTI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0960">Knottin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027005500" evidence="7">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Agouti" evidence="8">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="118"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013925322.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15407" checksum="10F9E66AC2CE5C5E" modified="2020-10-07" version="1">MLRQLLLSLGVLQVTLAFLASGQGHLDERGPILEETGRPSSPGLEMVKEIHIPDFKQVALETQRVQDDLAPAASMMMISAALQAQDREGRSLRRCVRVQESCLGHKLPCCDPCATCYCRFFNANCFCKKFSSTFPCGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMP1</accession>
    <name>A0A6I9YMP1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106551480</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925065.1">
        <property type="nucleotide sequence ID" value="XM_014069590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMP1"/>
    <dbReference type="GeneID" id="106551480"/>
    <dbReference type="KEGG" id="tsr:106551480"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="391"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="414"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013925065.1"/>
        </source>
    </evidence>
    <sequence length="424" mass="48012" checksum="4914DA9215DCFECE" modified="2020-10-07" version="1">MTEILKQKDSELTNFKVAAGTLDASAKIYAVRVDAVHADVYRVLGGLGRDAAPAETENSQDTGGEKEDLDHSKKRSIKRKHLYKTIEKNLNNINVSETERKCESDPMFQKTASSFDECSTAGVFLTTLHTHSFLSEVLFDSTVVPLPSSGTSEIPSSSHVKVTDLKSILLRCVENSTICPSLSGFLFTQWNSETHNESVSALLDKFKKSDQAFDINAETDNEYLEDRMEDDFDADVLDKTMARDLGEFSEKLESCRIAPELSRENQVSTKEGDIGSMCLQLSMNPSEYSYFSPRIMSMWAGPEHWRFKPHHKGDAKSERVSKRRTVKKVFEINFDEEIDFELHFRKTRAATVLSKSTLQSQNKKSNTLPKDFHYDPDNLTRLFLKPQIRLSNMPLQGRSPSHNDDDEVGEYDYNNPNDTSNFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMV3</accession>
    <name>A0A6I9YMV3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tropomyosin beta chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106552077</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925743.1">
        <property type="nucleotide sequence ID" value="XM_014070268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMV3"/>
    <dbReference type="GeneID" id="106552077"/>
    <dbReference type="KEGG" id="tsr:106552077"/>
    <dbReference type="OrthoDB" id="128924at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925743.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="21700" checksum="7BCC3F9105C66B77" modified="2020-10-07" version="1">MEAIKKKMQMLKLDKENAIDRAEQAEGDKKQAEDRCKQLEEEKQGLEKKLKGTEDEVEKYSEAVKEAQEKLEMAEKKAADAESDVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMZ2</accession>
    <name>A0A6I9YMZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Enoyl-CoA delta isomerase 1, mitochondrial</fullName>
            <ecNumber evidence="5">5.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">3,2-trans-enoyl-CoA isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20 21">Delta(3),Delta(2)-enoyl-CoA isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Dodecenoyl-CoA isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ECI1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Key enzyme of fatty acid beta-oxidation. Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2-trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs. The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2E)-tetradecenoyl-CoA = (3Z)-tetradecenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:29847"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
            <dbReference type="ChEBI" id="CHEBI:61968"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:29849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(3Z)-decenoyl-CoA = (2E)-decenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:77195"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:195601"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:77196"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(3Z)-dodecenoyl-CoA = (2E)-dodecenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:23716"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:58543"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:23717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(3Z)-hexenoyl-CoA = (2E)-hexenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45748"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:85415"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:45749"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(3Z)-octenoyl-CoA = (2E)-octenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:46044"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:85640"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45228"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:45229"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 24">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.8" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013925344.1">
        <property type="nucleotide sequence ID" value="XM_014069869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMZ2"/>
    <dbReference type="GeneID" id="106551720"/>
    <dbReference type="KEGG" id="tsr:106551720"/>
    <dbReference type="CTD" id="1632"/>
    <dbReference type="OrthoDB" id="1696280at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000034">
        <property type="entry name" value="Enoyl-CoA delta isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF45">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11 26" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00068317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00082088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013925344.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="32294" checksum="9E4F86E823E2BB0F" modified="2020-10-07" version="1">MAAAAMAAGRLAGAALRCPLTRLAFPAGQRRRMSGASPVLLELDERAGVAVLRLQQPPVNGLSLAMLTELCLALQKLEDRRACRGLVLTSAVPGIFSAGLDITEMCGRSEERLSEFWRAVQELWILLYGSRLATVAAVNGSAPAGGCLMALSCDYRIMADNPKYGIGLNETRLGIVAPFWFKDSLLNTVGHRATERSLQLGLMYSPQEALKIGLVDEIVPEDKIQARAAEVVAQWLAIPDHARQLSKSALRKPVLDHMLMHREADIQDFVRFISRDAIQKSLQKYMEMLKQKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN43</accession>
    <name>A0A6I9YN43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thymidylate synthase</fullName>
            <ecNumber evidence="2">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TYMS</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013925576.1">
        <property type="nucleotide sequence ID" value="XM_014070101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN43"/>
    <dbReference type="GeneID" id="106551937"/>
    <dbReference type="KEGG" id="tsr:106551937"/>
    <dbReference type="CTD" id="7298"/>
    <dbReference type="OrthoDB" id="766at2759"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000002">
        <property type="entry name" value="Possible thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002497">
        <property type="entry name" value="PRK01827.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="9">
        <location>
            <begin position="32"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925576.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="35643" checksum="617F47C11530A930" modified="2020-10-07" version="1">MPADLDVQGTPALPQEKPHAASGSSPCHGEFQYLQQVERILRFGHKKEDRTGTGTLSIFGMQARYNLRDDFPLLTTKRVFWKGVLEELLWFIQGSTNAKELSAKGVKIWDANGSRQFLDKQGFFSREEGDLGPVYGFQWRHFGAEYKDMNTDYTGQGVDQLQQVIDTIRNNPDDRRIIMCAWNPKDLSAMALPPCHALCQFYISNGELSCQLYQRSGDMGLGVPFNIASYSLLTSMIAHVTGLKPGEFIHTLGDAHIYLNHIEPLKIQLQRQPRPFPKLKILRKVENINDFRADDFEIEDYHPHPAIKMEMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN65</accession>
    <name>A0A6I9YN65_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein 12</fullName>
            <ecNumber evidence="2">1.8.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TXNDC12</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[protein]-disulfide + 2 glutathione = [protein]-dithiol + glutathione disulfide</text>
            <dbReference type="Rhea" id="RHEA:21064"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.8.4.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:21066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.4.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013925712.1">
        <property type="nucleotide sequence ID" value="XM_014070237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN65"/>
    <dbReference type="GeneID" id="106552050"/>
    <dbReference type="KEGG" id="tsr:106552050"/>
    <dbReference type="CTD" id="51060"/>
    <dbReference type="OrthoDB" id="262308at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019153">
        <property type="term" value="F:protein-disulfide reductase (glutathione) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02959">
        <property type="entry name" value="ERp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000099">
        <property type="entry name" value="thioredoxin domain-containing protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051099">
        <property type="entry name" value="AGR/TXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037462">
        <property type="entry name" value="ERp19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15337">
        <property type="entry name" value="ANTERIOR GRADIENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15337:SF10">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027103061" description="Thioredoxin domain-containing protein 12" evidence="10">
        <location>
            <begin position="24"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="14"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013925712.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="19215" checksum="86327BA018E2ED78" modified="2020-10-07" version="1">MRVHLASLSPLLFSSFCHWFVEAAAEKAFGKGFGDHIHWRTLEDGKKEAAASGLPLMIIIHKSWCGACKALKPKFAESKEISELAHNFVMVNLEDEEEPKDEAFSPDGGYIPRIIFMDPSGKIHPEIINEKGNPNYKYFYTSADQVIQGMKVAQEKLTGDAFKKKHFGDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN84</accession>
    <name>A0A6I9YN84_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC106551746</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106551746</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925369.1">
        <property type="nucleotide sequence ID" value="XM_014069894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN84"/>
    <dbReference type="GeneID" id="106551746"/>
    <dbReference type="KEGG" id="tsr:106551746"/>
    <dbReference type="OrthoDB" id="8939548at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000013">
        <property type="entry name" value="Collagen, type I, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24637">
        <property type="entry name" value="COLLAGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026686407" evidence="2">
        <location>
            <begin position="27"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="3">
        <location>
            <begin position="35"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925369.1"/>
        </source>
    </evidence>
    <sequence length="839" mass="90737" checksum="7111132D6883F53C" modified="2020-10-07" version="1">MFSFLDSRTLVLLAAIQVILLEVVNCQNEDKEEAGSCVQDGQRYSDKDVWKPEPCQICVCDTGTVLCDDILCEEMRDCPRPEIPFGECCPICPTDQPTASGQPGPKGQKGEPGDIKNVVGPKGPPGAQGPSGEPGPRGERGDKGERGAMGPRGPPGLPGKPGEDGEAGKPGKSGERGLPGPQGARGFPGTPGLPGVKGHRGYPGLDGSKGEAGAPGAKGEAGSSGENGSPGPMLQFSDGIEGVHTSTMASANSVQCWVLVAFQVKEDVLAHLVLLDLLALLVDQGFQELQGQRVKLVPLALVALRVPKALEVNLDPLVLRDLQDLQATLEVTGSLVPKDQPEVLALLVLLVSLAHVGHLGHKVQQDLWAPKVRRESPELQASKVNKAPRVNLALGDHKEPLGQPAKKGREEDVVNQELQDQLDHLERGVLPENVAPLALGDSKVPLEILEGLENLACLAPGSNMLSQFCGIHNSQSFQITFYVHKSPFLNDLDSENLYRFKFVDLPHFQCQVVSLVALGMLVLKAKLAHLVFRVKMDGLAHLVLKVLVVSLVSWVSQAPKVLMVKAVKLARKGYLVLLDYEVFLAKMAYSVVVVVSDDDDDICRGLLEREVNKVPLDHLDSKDFQDPQALQVKVEKQVTKVFLEKLVLLVLLVPEVNVASQENADLQVPKVWVVLEGFPEPLVLMDPRVQLALLGLLEPKAPQACRECLVREELEVFLDQRETGVMLVRKAQKVLLAKMAAEVNQVLLAQVELLVLGVPLVSVEKPDPLAPLGLLVPLVLMASLVLKASKVDLARKEMLGPLVHKGHLVLLVHSFWHAVDQDMLTWADGPCFKVQVQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNB9</accession>
    <name>A0A6I9YNB9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RNF125</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013925762.1">
        <property type="nucleotide sequence ID" value="XM_014070287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNB9"/>
    <dbReference type="GeneID" id="106552103"/>
    <dbReference type="KEGG" id="tsr:106552103"/>
    <dbReference type="CTD" id="54941"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034734">
        <property type="entry name" value="ZF_C2HC_RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18574">
        <property type="entry name" value="zf_C2HC_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51803">
        <property type="entry name" value="ZF_C2HC_RNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="43"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC RNF-type" evidence="12">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925762.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="27688" checksum="729B7D6F44D52414" modified="2020-10-07" version="1">MGSVLSGDGRETARTPDVGNLQGSIQFPQPGDVEKQQIPSFDCAICLEVFDQPVRTKCGHVFCHTCITTCLRNHTWTCPYCRAYLPSEGIPATDIIKMMKNIHQNCTECKTQVCLSEMRAHLKTCDQYIEKYGPLQELAYSETRYPCPLCQNELDENDLLDHFLTFHRTERQPVYCPICRLLPSGGPSYYIRNFIRHLQLRHTFYYEDYIDINIVEEVLIERVLDRSVLDYVHLNNPGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNE3</accession>
    <name>A0A6I9YNE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">MED10</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925676.1">
        <property type="nucleotide sequence ID" value="XM_014070201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNE3"/>
    <dbReference type="GeneID" id="106552020"/>
    <dbReference type="KEGG" id="tsr:106552020"/>
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    <dbReference type="OrthoDB" id="337270at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019145">
        <property type="entry name" value="Mediator_Med10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345:SF13">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09748">
        <property type="entry name" value="Med10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925676.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="15492" checksum="9F20395D0EE09B10" modified="2020-10-07" version="1">MAEKFDSLEEHLEKFVENIRQLGIIVSDFQPSSQAGLSQKLNFMVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELTKVFPEDMAKYKAIRSEDPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNS9</accession>
    <name>A0A6I9YNS9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein very KIND</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">KNDC1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925932.1">
        <property type="nucleotide sequence ID" value="XM_014070457.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106552233"/>
    <dbReference type="KEGG" id="tsr:106552233"/>
    <dbReference type="CTD" id="85442"/>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032045">
        <property type="term" value="C:guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000529">
        <property type="entry name" value="Kinase non-catalytic C-lobe domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000013">
        <property type="entry name" value="Kinase non-catalytic C-lobe domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011019">
        <property type="entry name" value="KIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029899">
        <property type="entry name" value="KNDC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21560:SF0">
        <property type="entry name" value="KINASE NON-CATALYTIC C-LOBE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21560">
        <property type="entry name" value="VERY KIND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16474">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00750">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51377">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="KIND" evidence="7">
        <location>
            <begin position="25"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="KIND" evidence="7">
        <location>
            <begin position="508"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="1351"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="1559"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="471"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="763"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="995"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="763"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="817"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="999"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925932.1"/>
        </source>
    </evidence>
    <sequence length="1839" mass="207796" checksum="ECD5DBEF87B10766" modified="2020-10-07" version="1">MGEFAEEEGCDFDPLPALSEDEENVSLADVLSLQDSCLTEQDIWAVCLECSQSLKNVVHSTVFHTLCITPDTLAFNTNGNVCFMEQISDDPEGAFVPPEIDETGNTFEAHLYSLGSTLTAALEYTMETGTEPEFSQDLYALLGQMQKENPKERPDIEVVISLCKEKLKVSSPSDICQTLSATGRKILSIESYGTFQDSCDDIWEGTFYESPLPNEERPDDNVAKTITASAEDSTFARSESFRGPYAPNDGNSDLKTMKINKKEERVCCQSISTWVTKSQEGNSFTKRNKELDAKPYATGAERDSFERYCLRKIRTFPSVPFNSETHSRACLSFIGHQASLTRKPCQLQPGDTILEGPLIQCDHPESKSASLPHNNFGELCLSPNTSPGYPNHMTPSDDREAVDIFPRCCAISETSDQNNPADETSPECNALENCRVREFRSEAPLKSGQNCLFYINKMSSTFMASKEDGCNSSVLSSPEEKRSERTDCWTEDREYDDGDSETRNNECVFLKHFLIQYGKPLKDYELWALCHECLLTLETYTDYPAYLCLDSVVINPDGRISFASCGNEESLDGFYLAPETAEEGFVTEKACIYCMAAILWRAARSNFPPDHKLVLPGKLKHFLLSMARSKAEDRPSLADAIKICRSYLLEQNISSRRILEVLAKVAFQDFREADSLTDSWPFQKESCPGNTNLGFLPMSNESKLVAVKGPVPCQSPLNRERITLPNAFTSSATHFKPIILHQNINHKIDAIATPVVECETMTRIPSVSSRNPTPSSNDNENKTGEILNLSTPCMMPGTLEQSEEEQKMLQEKDSEEQSPSSSRSPDPNLSLPDSLDVSQEKCILEAPPSANPSSTVPSSVTFVNNFLLKQDPETRVLTFVPVQIAISEQIPNKPLRSKATYSCCPNLHLLLSEPAMAYDAHGALQANSSLSDAIKDQFGDVQNKCWGIKAKMGDLTPTTINKETQTKCYESNPCLVSCKERNSDTEKSLLFSDHAQDPSLDQNPGSLFPGTSQQNKGTQNVALESIVQLVQQEFAFDKSQDITEALAIGKYIFTLKDLQYNTFCNAVSEKFCELYWEEKLLANLYGAANGRIPVPERINEPQHLSKTFQSEPNTFLSGSEEAEDHVAGMDSEEKEVEPQTLLVDIQTEGECLQTEKRCSGPSVAETEQKQEEIQERVEEDTCSSPSCPSGFSPGWRSAFYGSEWFSLDVHSYFRKLGKQKTHGTQNIDAKKMELDQLIMIETRNYRKSISFYQRLLYKERSRKGGEIRTFLPKLERQIEEMKSKTRFLELVKNYLQIMYAEKWGVEPCNFLTVVNMGQNEMLDIGALDDVLIFYHINKTPSEAQYNDNQNCLRNLQAGTPLGLMACLYSRNAFLKGYVQQFLFTFRYFCSQEELLQFLLDRIRITLPSSSPESSSLFSKIYHRSFSILHAWIEDCYTVDFSINTNLMRILKDFIISQVVPLNGCGRQLLSLLNNISSEKERKVSRFCKMEEWEQGMELHETRSLRFSRKRDSGNVLQKHLSWRLSKGPEPQVCHRPTTEELSGFCIKIAEAPYFLNEYTAQQLCSQLTLLEQELFHKCHPVHFLNSRLLGVKDKNVPLPKASSSELLSTPTYNLFANNCVQDDHLLQLLKYADNVSTWVAAEIVTSCSSKVQMNWVSKFLLTAKCCYKQRNFATAMQILRGLENLIVRQLPIWKNMPSKVSDILEELKAVEVFLKSDSLCLMEGERFKTSSTIPSAHILAMHIQQLETGGFTMRNGTYKWTKLRNIAKVVSQVQAFQENPYVFPPDCQLQDFLRQRITFLNDADIFTLAADNYANFHQKPEKQSRKIQDALHRMKAMFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNW8</accession>
    <name>A0A6I9YNW8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sperm-tail PG-rich repeat-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">STPG2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925410.1">
        <property type="nucleotide sequence ID" value="XM_014069935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNW8"/>
    <dbReference type="GeneID" id="106551779"/>
    <dbReference type="KEGG" id="tsr:106551779"/>
    <dbReference type="CTD" id="285555"/>
    <dbReference type="OrthoDB" id="406368at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF60">
        <property type="entry name" value="SPERM-TAIL PG-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013925410.1"/>
        </source>
    </evidence>
    <sequence length="468" mass="52149" checksum="ACF78633854E3E35" modified="2020-10-07" version="1">MPSARKIAGCIKYFRKSEAPSIPSQGQAFGYEEAEDGTLIKFNQPVRDNTLGPAYYQSVYNETYPTLKYKGVHFGSLREKRPEFKPQEGPGPADYDIMPESATHCENVNIKKEDKKKCVLYIPRYHEEIVLQEGKKGVPGPGQYNIKSEFGKAGSIMKSEAQHVPFLSLSERFAPLKPSTPAPGTYDETRSALECLKRPCRSKSIPFGHTSVRFTSDSRLQTSPGPAFYNILNYRIAAPHLKKVLAETKKKGAFGSTVPRLLYLVNRQAFATPGPADYQMKQVVEESHKKKKELSVFVSTIERIPVIHSAVPPPGSYEVHKSFEKSQGKSEYMPPRTVVARRRHASFLSGTFREEALKAETEIPGPGTYSPVDQPGAKLSPGTTVLVSQEARFKDAKSTSPGPASYVLSPLLRDTVLKRTYNVSLNNPVMIRVRSAIFKSARDREDLFCTSDQQDSKMQNETSVSDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQJ5</accession>
    <name>A0A6I9YQJ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RISC-loading complex subunit TARBP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106552912</name>
        <name evidence="11" type="synonym">TARBP2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation. For some pre-miRNA substrates, may also alter the choice of cleavage site by DICER1. Negatively regulates IRF7-mediated IFN-beta signaling triggered by viral infection by inhibiting the phosphorylation of IRF7 and promoting its 'Lys'-48-linked ubiquitination and degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TARBP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926793.1">
        <property type="nucleotide sequence ID" value="XM_014071318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQJ5"/>
    <dbReference type="GeneID" id="106552912"/>
    <dbReference type="KEGG" id="tsr:106552912"/>
    <dbReference type="OrthoDB" id="10056847at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070883">
        <property type="term" value="F:pre-miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098795">
        <property type="term" value="P:global gene silencing by mRNA cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903798">
        <property type="term" value="P:regulation of miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070921">
        <property type="term" value="P:regulation of siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046782">
        <property type="term" value="P:regulation of viral transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070922">
        <property type="term" value="P:RISC complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19890">
        <property type="entry name" value="DSRM_TARBP2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10844">
        <property type="entry name" value="DSRM_TARBP2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19893">
        <property type="entry name" value="DSRM_TARBP2_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000019">
        <property type="entry name" value="RISC-loading complex subunit TARBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000120">
        <property type="entry name" value="RISC-loading complex subunit TARBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000018">
        <property type="entry name" value="RISC-loading complex subunit TARBP2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03034">
        <property type="entry name" value="TRBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051247">
        <property type="entry name" value="RLC_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028605">
        <property type="entry name" value="TRBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044469">
        <property type="entry name" value="TRBP2_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044470">
        <property type="entry name" value="TRBP2_DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044471">
        <property type="entry name" value="TRBP2_DSRM_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205:SF1">
        <property type="entry name" value="RISC-LOADING COMPLEX SUBUNIT TARBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 11" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="6 11" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="DRBM" evidence="13">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="13">
        <location>
            <begin position="159"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="13">
        <location>
            <begin position="288"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013926793.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="39064" checksum="1C2ED9E2C8C28357" modified="2020-10-07" version="1">MSEEEEAEEPCGSPRSCGSFPCIEQMLASNPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGDISCTGQGPSKKAAKHKAAEVALRLLKGGNMLEPTPLDEASSLSLEPLALTTVPVAVPVPILPAASPRSSPLDIKSPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLVRIHNVPLDPREGSEAEMEEDQFSIIAGTKLDGMKSRGSGCTWDSLRNSVGEKILHLKNHRIFNTGFCNLLKELSEEQSFDISYLDIDETSISGLYQCLVELSTQPATVCHGSANSRTAARADAARNALQYLKIMAGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS43</accession>
    <name>A0A6I9YS43_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CD276 antigen-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106552885</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926754.1">
        <property type="nucleotide sequence ID" value="XM_014071279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS43"/>
    <dbReference type="GeneID" id="106552885"/>
    <dbReference type="KEGG" id="tsr:106552885"/>
    <dbReference type="OrthoDB" id="8897154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000438">
        <property type="entry name" value="CD276 antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF155">
        <property type="entry name" value="CD276 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="21"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013926754.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="25968" checksum="7136B4E3EE9E013D" modified="2020-10-07" version="1">MSGQFYPEYGEVLSVVLSSSPSGAVEIKVSEDPVVALVGHDAILHCSFSPEANFSLNELSLFWQVTDTNQMVHGFTEGRDQPAYQGSTYANRTALFYDQLAHGNMSLLLRRVQISDEGSFTCFVKVRDFSRAAVSLQMAARSFPRVIPVQGVGNHSVPPTTPFSLLCLLPGQHLLFPAVALWVTVGLSICLVVLLMVLAYVCQKKIRQSCKEEKAKAALQPLKNMDVKEEMAEEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTV8</accession>
    <name>A0A6I9YTV8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 420-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106553937</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928003.1">
        <property type="nucleotide sequence ID" value="XM_014072528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTV8"/>
    <dbReference type="GeneID" id="106553937"/>
    <dbReference type="KEGG" id="tsr:106553937"/>
    <dbReference type="OrthoDB" id="9043260at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000508">
        <property type="entry name" value="Myeloid zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000739">
        <property type="entry name" value="Zgc:171418 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000389">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000247">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000038">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF15">
        <property type="entry name" value="REDUCED EXPRESSION 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013928003.1"/>
        </source>
    </evidence>
    <sequence length="548" mass="64063" checksum="0E6B61229842B83F" modified="2020-10-07" version="1">HQKIHTGEKPHQCLECGKCFRRKSTLRNHERIHTGEKPYKCWECGKSFSQKAHLCSHEKIHAGIKPYKCFECGKCFRRKSTLRNHERIHTGEKPYKCWECGKSFSRKSTLSSHEKMHAGIKPYKCFECGKCFTQSSGLRSHVKTHTGEKPHKCEECDICFSQKSYLLRHQKIHTGEKPYQCLECGKCFREKSSLRIHERIHTGEKPYKCWECGKSYSHKQNLSIHEKIHAGVKSYKCFECGKCFTQSSGLRNHWKKHTGEKNEKCLECGKCFTLKGSLVQHQRRHRGEKPYECPECEKRFVHSYNLQKHQEWHKEKLPYTCGECGKGFRAQREVQIHQRSHTGEKPYKCAECGQCFSRKHHLGKHQRVHTGETPYSCVECGKCFAHKEGLWIHHKIHTGEKPHQCNECGQSYRTASILSRHVKTHTGEKNYKCLDCGKVYAHSSSLRSHSRIHTGEKPYKCLQCGRCFARRDTLVKHQRLHTGEKPFKCLECGKCFTQLSLLRMHTRHHTGVWPYKCAECEKPFHCKSKLSLHQKIHSRDKPTVFFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU21</accession>
    <name>A0A6I9YU21_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BTB/POZ domain-containing protein KCTD17-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928073.1">
        <property type="nucleotide sequence ID" value="XM_014072598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU21"/>
    <dbReference type="GeneID" id="106554000"/>
    <dbReference type="KEGG" id="tsr:106554000"/>
    <dbReference type="OrthoDB" id="1244179at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045724">
        <property type="term" value="P:positive regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2000:FF:000001">
        <property type="entry name" value="Potassium channel tetramerization domain-containing 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14958:SF24">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14958">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERISATION DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928073.1"/>
        </source>
    </evidence>
    <sequence length="172" mass="19386" checksum="23D8E65D58EE0C4B" modified="2020-10-07" version="1">MAEEGVLEEAEFYNIGSLIRLIKDRLEEKDYTITQVPPKHVYRVLQCQEEELTQMVSTMSDGWRFEQLVNIGSSYNYGNEDQSEFLCVVSKELCSSPSGLCSESSRKLKVPPAATQNPEEQQDPEQEQQKDEELQLQDIEEQEEAPVQADGGGTSKEGAWNACVGIKQYTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVQ9</accession>
    <name>A0A6I9YVQ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proteasomal ubiquitin receptor ADRM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ADRM1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADRM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928104.1">
        <property type="nucleotide sequence ID" value="XM_014072629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVQ9"/>
    <dbReference type="GeneID" id="106554029"/>
    <dbReference type="KEGG" id="tsr:106554029"/>
    <dbReference type="CTD" id="11047"/>
    <dbReference type="OrthoDB" id="340431at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2020.20:FF:000001">
        <property type="entry name" value="Proteasomal ubiquitin receptor ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEUBAD" evidence="5">
        <location>
            <begin position="114"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="21"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928104.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="24534" checksum="4B50C03FBF961ADB" modified="2020-10-07" version="1">MSHNQLMQLIGPMGLGGLGPGLASLLGSGGPPTSSSSSSSRSQSAAVTPSSSTSSTRVTPAPAAPAATTATATATATSPSPAPSSGNGTSTATSPTQPIQLSDLQTILATMNVPAGTGGPQVDLSSVLTPEIMAPILANTEVQERLLPYLPSGESLPQTAEEIQNTLTSPQFHQALSMFSAALASGQLGPLMSQFGLPAEAIDAANKGDVEAFAKAMQNNVKSDQKEGEAKEKKDEEEDMSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YW02</accession>
    <name>A0A6I9YW02_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106553738</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927775.1">
        <property type="nucleotide sequence ID" value="XM_014072300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YW02"/>
    <dbReference type="GeneID" id="106553738"/>
    <dbReference type="KEGG" id="tsr:106553738"/>
    <dbReference type="OrthoDB" id="10040445at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.810">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010195">
        <property type="entry name" value="Uncharacterised_peroxidase-rel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01926">
        <property type="entry name" value="peroxid_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35446:SF2">
        <property type="entry name" value="CARBOXYMUCONOLACTONE DECARBOXYLASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35446">
        <property type="entry name" value="SI:CH211-175M2.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="1">
        <location>
            <begin position="96"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927775.1"/>
        </source>
    </evidence>
    <sequence length="249" mass="28161" checksum="8EBD52CB94AD5AE7" modified="2020-10-07" version="1">MIKRFLLSGFLQLHRCSGGSVSFLPLSVSGNLWGRSQSVALNHPKSTEGETTTKEIGRYPIPKKTDLPYDIVELMEEMEVKTGFLPNVFKVMSHRPAEFRAFFAYYNAIMNKETGNLSKADKELIIVATSIVNKCPYCVIAHSALHRVYSKKPTLADQVAVNWQLADLSDREVAILDFALAICRADNITEEHFQKLEARGFDREDAWDIASISAFFAMSNRLAHFIDLHPNPEFYIMGRIPRDKKNSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXA2</accession>
    <name>A0A6I9YXA2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein FAM134C isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="14">Protein FAM134C-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">FAM134C</name>
        <name evidence="14" type="synonym">LOC106554171</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RETREG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928240.1">
        <property type="nucleotide sequence ID" value="XM_014072765.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928256.1">
        <property type="nucleotide sequence ID" value="XM_014072781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXA2"/>
    <dbReference type="GeneID" id="106554153"/>
    <dbReference type="GeneID" id="106554171"/>
    <dbReference type="KEGG" id="tsr:106554153"/>
    <dbReference type="KEGG" id="tsr:106554171"/>
    <dbReference type="CTD" id="162427"/>
    <dbReference type="OrthoDB" id="10029527at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057282">
        <property type="entry name" value="RETREG1-3-like_RHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043384">
        <property type="entry name" value="RETREG1/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28659">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28659:SF1">
        <property type="entry name" value="RETICULOPHAGY REGULATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24456">
        <property type="entry name" value="RHD_RETREG1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="RETREG1-3/ARL6IP-like N-terminal reticulon-homology" evidence="11">
        <location>
            <begin position="58"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013928256.1"/>
        </source>
    </evidence>
    <sequence length="475" mass="52521" checksum="11096009072E0937" modified="2020-10-07" version="1">MLGEGRMAAAEAAGRAAGSSSGTNHGGGRGLVTDAQGPSSEREQRVRALSAFLRTHLGPYEPILTALQATLTWEKPARSALGWVAAHAAFGFFALTSLHLIFVIAFTLILIVCLDQWKNRIWPEIKAVRPDQLDNESWGFVHPRLLSVPELCHFLAKGWITGDSFMKSLLSFKEQNPGKFCLLACGALTFLTVLGYYIPGVCLAYLIMLSFLLWPLAVYHNLNQHLYTKLQPALQRLDFSVRGYMMSKHKERQSRHHIWREHPAGADESDSEEELAAFCPKLDDAVVAKELTISDSEHSDAEVSYTENGTFNLSRGQTPLTEGSEDFDGHSDPEESFARDLPDFPSINPEVTGIDDEDDTSIGIPSLSLRNQAQEQNQQSHKQEEVTSEFLLSELPLMHNLTDDLAGFVTRGMIQLALSGTSQSVAAHSNRQQRSAKAFLRTSSSELDTDAEGDDFELLDQSELNQMDPSNSRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYT5</accession>
    <name>A0A6I9YYT5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sodium/myo-inositol cotransporter 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106555528</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929873.1">
        <property type="nucleotide sequence ID" value="XM_014074398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYT5"/>
    <dbReference type="GeneID" id="106555528"/>
    <dbReference type="KEGG" id="tsr:106555528"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF171">
        <property type="entry name" value="SODIUM_MYO-INOSITOL COTRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929873.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21093" checksum="EF252D41A74D23F6" modified="2020-10-07" version="1">MVIGLIRMVLDFVYLQPGCGESDDRPAVVKYVHYLYFSMILGVITLILVVAVSLLTEPPPEEMISRLTWFTRKDVPRKTDVPIPSLSIPVKGIHEAGPSPAGQLEITDGPENTFQNHSGPLDTKRKESKFMSLFLWVCGMESRDKDSPGTPAEPKAAVTAILDEAPLVKHILNINLILCISAGVFLWAYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1C4</accession>
    <name>A0A6I9Z1C4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 186</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMEM186</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM186 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929871.1">
        <property type="nucleotide sequence ID" value="XM_014074396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1C4"/>
    <dbReference type="GeneID" id="106555526"/>
    <dbReference type="KEGG" id="tsr:106555526"/>
    <dbReference type="CTD" id="25880"/>
    <dbReference type="OrthoDB" id="6147888at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026571">
        <property type="entry name" value="Tmem186"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13603">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13603:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929871.1"/>
        </source>
    </evidence>
    <sequence length="235" mass="26898" checksum="F41D2DCBF3462C64" modified="2020-10-07" version="1">MKSLVSCTRQHLALFKPRCLQNSLALPLWKRGHVGLHSFQSPTILQQGGSPCTPGLCRVQPRWLSTVDLSQQDPKPPSSTENTEKFTVIYRFPAIRLLKLLSRLKVVQTGFGLLVLPPLWYFNGLNQVTLVTGLFSFTLVFLYTLSFFLERVIGLMYLNESGTLLKISHLTFWGKRKNFCCPVDSVVTLGDKDRDSNRFLLKFRQCDQKKVLYFSLVLGQVVDHEAFDKVFRCYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2C9</accession>
    <name>A0A6I9Z2C9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">LOW QUALITY PROTEIN: zinc finger protein 721-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106555962</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930384.1">
        <property type="nucleotide sequence ID" value="XM_014074909.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555962"/>
    <dbReference type="KEGG" id="tsr:106555962"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002093">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 354C"/>
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        <property type="match status" value="47"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="Znf_C2H2_type"/>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
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    <sequence length="1576" mass="181689" checksum="3C02042CEC2C8D91" modified="2020-10-07" version="1">MTNAKDGMETFGIRMELESHERNNRNNERLPSTDAPMQDFLTQEEKIRKKIIGKSVKLIKDKLHVSEHYLTQNKGEDAIRHNGKNYNGTFIHPLGNNILTSQKLIHSKKDPYKCLESGKHFRTSKQLTSDERIHTVKELYKCREYGNRFSSGGNLIIHKIIHTGEKPYKCMECGKTFTRSNGLRIHKMIHTGEKPYKCMECGKTFTHSNGLRYHKMIHTGQKPFKCMECGKTFAQRGHLISHKMIHTGEKPFECMQCGKTFTRIYELTIHKKRHMGEKPYKCMQCGKTFTRSNELTIHKKNHTGEKPFKCMECGKTFALSSVLRSHKRIHTGEKPYKCMECGKTFALSNRLAIHKKIHTGEKPFKCMDCGKTFAQHCHLISHKMIHTGEKPYKCIECGETFTQKSSLISHHVIHTGEKPYKCMECGKTFARTCNLISHKMIHTREKTFKCTECGKTFAQSRYLASHKKIHSEEKPFKCMECGKTFTKSNALTSHNRIHTGEKPYKCMECGKTFAQNGNLTAHKRIHAREKVSNNSNSTSDLSTTPQSFIAVFEHLQSQHIVPRNLGPHYRLNQSRAVGNNGQENQDSCELFQKNPNECLESEKHCRTSRQRTSHEGIHTGEKQCSEAPVAQAAQCLQVAKKKPFKSMEYGRIFIHSNGLTIHKKRHTGEKPYKCMECGKPFIRESSLTSHHVIHTGEKPYKCMECGKTFSRNYDLNIHKSLHTGEKPYKCLECGKTFAQRSYLASHKKIHSEEKPYKCMQCGITFSRNYNLNIHKSLHTGEKPYKCLECGKTFAQRSYLASHKKIHSEEKPFKCMECGKSFAQNSNLTAHNRVHTGEKPYKCMQCGKSFAQNGNLTSHKRIHTGEKPYKCIECGKTFIHNNGLKNHKTIHTGEKPYKCMECGKTFALSNRLAVHKKIHTGEKQFKCMECGKTFTQSGHLISHKMIHTGEKPFKCIECGKTFAHRGNLISHQVIHTGEKPYKCMSCGKTFARSGQLRNHKKIHTGEKPFKCTECGKTFTQRDNLICHKMIHTGEKPCKCMQCGKTFTRSYELKIHKKRHTGEKPYMCMQCGKTFTRSYELTIHKRRHMGEKPYKCLQCGKTFTRSNELTIHKKIHTGEKPYKCMECGKTFARNGQLRNHKNIHTGEKPFKCMECGKTFTQSTGLRKHKTIHTGQKPYKCMECGKTFVYNSSLTNHKKIHTEEEPFKCMECGKTFTHSNVLRNHQKIHTGEKPFKCMECGKTFTQKGHLISHKMIHTGEKPYRCMECGKTFVQCSHLVSHKMIHTGEKPYRCMECGKTFVHHLVSHKMIHTGEKPYQCMQCGKNFTRSYELTIHKKRHTGEKAYKCMQCGKTFTRNNELTAHKKIHTGEKPYKCMQCGKNFTRSSELTIHKKRHMGEKLHKCMECGKTFIRSNELTAHKKIHMGEKPYKCMECGKIFTCSNERTAHKKIHMGDKPYKCMECGKTFTHSSVLRSHKKIHTGEKPYKCMECGKTFALNNRLTIHKKIHTGEKPFKCMECGKTFAQNGNLSSHKRIHTREKACSNRNSTSDLYPTPQSFIAVFEQFTKSAYYTQKSGSSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3K9</accession>
    <name>A0A6I9Z3K9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106556109</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC106556109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930556.1">
        <property type="nucleotide sequence ID" value="XM_014075081.1"/>
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    <dbReference type="KEGG" id="tsr:106556109"/>
    <dbReference type="OrthoDB" id="3169036at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Nascent polypeptide-associated complex (NAC), alpha subunit"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
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    </dbReference>
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        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
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    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAC"/>
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        <property type="entry name" value="NAC_AB"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="NAC-A/B" evidence="2">
        <location>
            <begin position="2440"/>
            <end position="2505"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1457"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1569"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2052"/>
            <end position="2115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2126"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2179"/>
            <end position="2360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2381"/>
            <end position="2409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2424"/>
            <end position="2450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="757"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="774"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1458"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2206"/>
            <end position="2221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2242"/>
            <end position="2257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2306"/>
            <end position="2317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2390"/>
            <end position="2403"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="2541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930556.1"/>
        </source>
    </evidence>
    <sequence length="2541" mass="275180" checksum="31480A91C902CBC3" modified="2020-10-07" version="1">MSKLGLRQIHGVNRITIRKSKNILFVIDNPDVFKSPASDIYIVFGEAKTIESDQMIPALLLPFRGSLLFEAESMEIVLFPQGEAAGNDALYDVEDDDDSASNSFLHSLSETSIDEGVDESFAYPDDTSQSSDSASNDGKDGVEQYAVVADIVVESKEDTERALLHVESESEMDISSDAYNTDEDTVHGTGEEQTRGETEEKGTPKEEKLIEDDIMKPPRSFDTTVVSRLQARPESPGGVSAKSISHLGSGDEDSVADSEDLTPPTQDDKEEDSLKEMVLSDVSPTHLPHSDVQPTDLESGLSECGECLIACFDTDDESDNQPPLSDTVEESPSVGPLAEEWMTQVCAGSVIPLGWNPISCPIELEDLEAVDNNDSSSLDIGARLEESEKRLMELLDQDGATSGSSTELELGDGELLGTSSEKERPFASLLESKEALLEQPEVAQTDTETTQDSLIVCLESEDELEEASSLDQINNNEDRAMMAFSEAKPDSHSLLVLSHTPENILTEDNGVLLDASAPLPXQMALFLPVKKKKEDRPQPEGASCDTTTSNAAAGSVALSECGQIVMEQAPKSGGTRPRSPEHSPGTDLLDARIVMGEETHCLRVAVQDILGVPTPGISETTSGEEMSNMGNEGLDAAFQGAPLPPVARGFGNLHACASVSPPGLLKSQELAGVGVFPLSQGEPMANVPSLSPTLAKDLPAELAESQLFLAKHPCSLDTELYFTAPSTPIRTLFPPLRQPPFSKDGLSEEQNEVDSDSLCSPPTSPSGSYITAEGGSWTSTGTASTSPSCSPNLLAESEALEAPTADEEALSELDFPEGEPKLSRVTCPTFGAFPRLSYNTFPSSYPAEECSVGEEEERIPSEEGWGCETIPLKLLPHEAEQTSPSWPPDDDECEVGADFSFTPLEEQARLLPSYDTEIGGFTKRGLEACPFSGPFTSVSEPRSELQGVFEAVPTEVGEHGGVQSQPPMSFPEDVVKTIESDQMIPALLLPFHGSLLFEAEYMEITLFPQGEVVENDALYGMEDNDSTSVSFLCSLSETSVKKGVDESFAYPDDTSQSSDLASDDSEDGVEQYTGVADTVVESKEDLESTPSQLESESEMDVSLDAYNMDEDTIRGTGEEQMTGESEEKGTPKEEKLIQEDPMKPPRSFDTTVVSRLQARPESRGGASSRSSCISHLGLGEEDSVVASEDLSLPTQDDKEEESHEEMVLSHISPRHLPHSDIQPTHLESGVSECGECLIACFDINDESDNQPPLTDTTEEFPSVGPLAEEWMDQVCAGSVIPLEWNSKSCPVELEDLEAVDNHDSSSLDISTRLEESEKRLLELFDQYGAASGNSAELEPGDSELLETSSEKEMPFVSLLESKGALLEQPEVIQADAKTTQNSLVVCLESEDELEEVSSLDQINNNEDRVMVVAFSEAKPDSHSLLALSNTPETILAEDNRVQESPLFSPHREEAADLQNWSSCGKQNSSVNPEIEDYSGHSSNDEAVVDDTITPNLSKLCLETGETEALSGQETQDSDRYLMPTHVEMGSRVEKTSEEDLDKAAETLHLSREELVETNNPLNMTKVVEDPRQQGLSKEGNADQGWGTPSEDVAAEIPPAEDLQMEGQLDSDYLTTTDQLGSKVTQQGEAHNLDPTEPPEKFATSDEIACHQEEICETTEEVDSEDRNGSSECWQMEDILIPEPQVVPRRVDSVESPRDVVYVPLLDTKTGMLKVVPGVLPAENIDQMVSSTTEDDLCEEHPEVQRTFAEALLQGLLPILETGHPLQKEKEVDTTLLSPDPLEESASYSYLECSFVACPEDVSNETMILAPSPDSGREEFATPDQTWGSQNMVVEESHSPTGLEAATTELEHLMVENVELECLDVPPNCSLEEVPPKQSTAVCLHYSVLAHMPLTTSHPAVKQRDVSPHQQIFLASEDKIYLSNLHKTQDSLSSRHVEMVQVESEASTLESSPTIPSQLEPSGALFQRDGDGATVSPMALEDQQEVTSVLQGSFGNLEEQRVESAHLISSLLVAEAQSRLGSLKENVLENLGGEHVEEVVEPKYSEELEAQRTLGGEETVDHKQSSWQSCKEAKAEDQAEMVEEGGTGEVLPVSEGSEVEDSQLQGSAPKEATQLGVENIVSAPGKDEAEVMDGSPPQSPPEDLPRLEAKRMANEKTMEEALEVMELEEAEMSHPMMDNLLTESTGHSSSEDAFSSLDSPIPLASRSIGTSSQETCPSQRPSSPAPEEVPVTLPPALRSQFPEVPPVSPLILPSPPSEGPVQGPSATSRLAPSTAGPCVEHTLLKESLLQDTRKPPVEALQSASSSGPGQLHQLPGGQDARGRNQLPGNRDSRVKYLGLAPEKRGSRVSFPPESSSSEERELETLREAGMMLLDEKTTLVGKRGHDLNHKGSSNDSESNEGSLPELEEPNLPVPRTAHVAQNQLTHSLGTGEESISKAKQSRSEKKARKAMSKLGLRQIHGVTRITIRKSKNILFVITKPDVFKSPASDIYIVFGEAKIEDFSQQVHKAAAAKFKVPVEHSPLITKAAPALTIKEGSEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3U1</accession>
    <name>A0A6I9Z3U1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556832</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931317.1">
        <property type="nucleotide sequence ID" value="XM_014075842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3U1"/>
    <dbReference type="GeneID" id="106556832"/>
    <dbReference type="KEGG" id="tsr:106556832"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000063">
        <property type="entry name" value="Kelch-like protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000086">
        <property type="entry name" value="Kelch-like protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF75">
        <property type="entry name" value="KELCH-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931317.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="34748" checksum="10946E1F330AAA4B" modified="2020-10-07" version="1">MKYHLLPERRTLMQSPRTKPRKSTVGTLYAVGGMDNNKAGATTIEKYDLRTNIWIQAGVMNGRRLQFGVAVIDDKLFVIGGRDGLKTLNTVECYNPKTKAWTVLPPMSTHRHGLGVTVLEGPIYAVGGHDGWSYLNTVERWDPQSQQWTFVANMSIARSTVGVAALNGKLYSVGGRDGSSCLSSMEYYDPHTNKWNMCAPMCKRRGGVGVATCDGFLYAVGGHDAPASNHCSRLLDYVERYDPKTDTWTMVAPLSMPRDAVGVCILGDKLYAVGGYDGQTYLNTMESYDPQTNEWTQMASLNIGRAGACVVVIKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4F5</accession>
    <name>A0A6I9Z4F5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Perilipin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106556983</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931532.1">
        <property type="nucleotide sequence ID" value="XM_014076057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4F5"/>
    <dbReference type="GeneID" id="106556983"/>
    <dbReference type="KEGG" id="tsr:106556983"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.340">
        <property type="entry name" value="Flagellar protein FliS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.170">
        <property type="entry name" value="Perilipin, alpha-beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF11">
        <property type="entry name" value="PERILIPIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109775">
        <property type="entry name" value="Mannose-6-phosphate receptor binding protein 1 (Tip47), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931532.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="25858" checksum="BE0EEE3CB58D52BF" modified="2020-10-07" version="1">MTDEELAKLATAVEGFEIGTVEKQKEQKSYFVRLGSLSSKLRHRAFQHSLVKLKRAKQGTQDSLSQLHQTIELIEHVKQGVDQKIQSGQEKLHQMWLQWQQKQAPEVAQKEPPKPQEIETQALEVTRGLTQQLQSATTTLVSNLQGLPAGLQEKVGLVRQNVDELRNAFMTAGSFQDLSGSILAQSREKVAKARQLTDELMDHVVQNAPLTWLVGPFTASGKPEGEEIEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4F9</accession>
    <name>A0A6I9Z4F9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 180-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930856.1">
        <property type="nucleotide sequence ID" value="XM_014075381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4F9"/>
    <dbReference type="GeneID" id="106556382"/>
    <dbReference type="KEGG" id="tsr:106556382"/>
    <dbReference type="OrthoDB" id="431588at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027914">
        <property type="entry name" value="DUF4456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444:SF14">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14644">
        <property type="entry name" value="DUF4456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4456" evidence="1">
        <location>
            <begin position="2"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930856.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="20337" checksum="63C654027FFCE0DB" modified="2020-10-07" version="1">MNVAEEFYKKDKHQITKPECLQETYDLCIDALGQKMLTYLFQSDEYHIACISEFRAQLKTFEEELPQVIRLAIGRILKDHEQLLAESTEQIRGHLQDQLKKWTAIKDKNMAQLHTSLGHPDNIPVLEALCQKEEKRQAEQVESILICTKRLECLGSTFREFMKKDDVGRISERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0E1</accession>
    <name>A0A6I9X0E1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CCN family member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cellular communication network factor 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein NOV homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106537580</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907237.1">
        <property type="nucleotide sequence ID" value="XM_014051762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0E1"/>
    <dbReference type="GeneID" id="106537580"/>
    <dbReference type="KEGG" id="tsr:106537580"/>
    <dbReference type="OrthoDB" id="365605at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005921">
        <property type="term" value="C:gap junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000046">
        <property type="entry name" value="Cellular communication network factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF37">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0303">Gap junction</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFC" evidence="18">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="17">
        <location>
            <begin position="161"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013907237.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29381" checksum="4FD3DF94167D1002" modified="2020-10-07" version="1">MGKTYLNGESFQPSCQLKCICMDGSIGCIPLCPDGAHLPPPDCPFHQWVKAQSKCCEGEWVCEGSRQKTPYKKVLLPISVYRNRFAHKPVLKLKPTLNDLQVNCLVQTTEWSVCSKTCGMGISTRVTNDNPQCQLEKESRLCLVRFCNIPLDRSIKRGKRCWRNPKSREAINFEFTGCTSIHSYRPKFCGSCTDNRCCMPRITSTVEVEFRCPEGESFVRKMMFIQSCSCHYDCSAGHDVFLASSHRSMVGDHVKLKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X196</accession>
    <name>A0A6I9X196_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar protein 56</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nucleolar protein 5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106538908</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the early to middle stages of 60S ribosomal subunit biogenesis. Required for the biogenesis of box C/D snoRNAs such U3, U8 and U14 snoRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with TCOF1 in an RNA-independent manner. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles; the core proteins SNU13, NOP56, NOP58 and FBL or FBLL1 assemble stepwise onto the snoRNA. Interacts with NOP1 and NOP58. Interacts with NUFIP1, RUVBL1 and RUVBL2; RUVBL1:RUVBL2 seem to bridge the association of NOP56 with NUFIP1. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with NOP2 and FBL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909024.1">
        <property type="nucleotide sequence ID" value="XM_014053549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X196"/>
    <dbReference type="GeneID" id="106538908"/>
    <dbReference type="KEGG" id="tsr:106538908"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000001">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000002">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="230"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="380"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909024.1"/>
        </source>
    </evidence>
    <sequence length="474" mass="52674" checksum="A461A9AD9F97F8B3" modified="2020-10-07" version="1">MGSVEERRLLHEDLRLLLETSLPAKRKKVLLGVGDPKIGAAIQEELGFPCQTGGVVAELLRGIRLHFHSLIKGLTAQAASKAQLGLGHSYSRAKVKFNVNRVDNMIIQSISLLDQLDKDINTFSMRVREWYGYHFPELIKIVSDNYTYCRLAKLIGNRKELSEESLEGLEEIVMDSAKAQAILDASRSSMGMDISPIDLINIESFSSRVISLSEYRKGLQEYLRSKMNQVAPSLSALIGEVVGARLISHAGSLTNLAKYPASTVQILGAEKALFRALKTRGNTPKYGLIFHSTFIGRAAAKNKGRISRYLANKCTIASRIDCFSEVPTAVFGDKLREQVEERLAFYETGEVPRKNLEVMKEAVEEASEAAAEIKRKLQKKERKKLKQEKKRLQALAAAAETQNNNSSPLLEPPEQENQAKAKRKKRKPQELEAEPANNSLGDEDAPLPKKKKKPPAVVVVEEPPAPQGKKAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X236</accession>
    <name>A0A6I9X236_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 249</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMEM249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909319.1">
        <property type="nucleotide sequence ID" value="XM_014053844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X236"/>
    <dbReference type="GeneID" id="106539144"/>
    <dbReference type="KEGG" id="tsr:106539144"/>
    <dbReference type="CTD" id="340393"/>
    <dbReference type="OrthoDB" id="5519333at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027861">
        <property type="entry name" value="TMEM249"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35442:SF1">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED AUXILIARY SUBUNIT TMEM249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35442">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15158">
        <property type="entry name" value="TMEM249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013909319.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="15042" checksum="A48A34826F2BD191" modified="2020-10-07" version="1">MSPVFVMEYYKDTVWKGLLGFVISLIASIFYFKTTWGYQDLSAFVIFALVASLWVLLSNLCKRRLIINHLRELYQFYIRGILWHEGPLHQIYVRLVGQRDGNPPPPPRGPGVSYPSPRPLATMEAKISVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X306</accession>
    <name>A0A6I9X306_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA decay activator protein ZFP36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Zinc finger protein 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZFP36L2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates with the cytoplasmic CCR4-NOT deadenylase complex to trigger ARE-containing mRNA deadenylation and decay processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909669.1">
        <property type="nucleotide sequence ID" value="XM_014054194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X306"/>
    <dbReference type="GeneID" id="106539403"/>
    <dbReference type="KEGG" id="tsr:106539403"/>
    <dbReference type="CTD" id="678"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900153">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007635">
        <property type="entry name" value="Tis11B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF174">
        <property type="entry name" value="MRNA DECAY ACTIVATOR PROTEIN ZFP36L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04553">
        <property type="entry name" value="Tis11B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="205"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="205"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="234"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="439"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909669.1"/>
        </source>
    </evidence>
    <sequence length="521" mass="54055" checksum="A8F2D60A58DF29B9" modified="2020-10-07" version="1">MSTTLLSAFYDIDFFCKTEKSLSNMLDKKAVGSPVGTSPSSNFTPGFLRRHSTNNLQALANGNIKFPSASLSAPSNFGSLKESGTGAGGCGSSSSTTALLNKENKFRDRSLSENGERSQHLMQQLHQQQVVVVAATSAGKGGGGGGGGGNGGGGGNGGGGAPINSTRYKTELCRPFEESGSCKYGEKCQFAHGFHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNADERRPAPANGSANPPAAQQQPPPPPPLQPNPPPHSSHPSHHHHHHHPSLHPPHAGSTGDLRAFAPSARDHPLGPGGTNGSNFGIPHPRGGGERPKLHHSLSFSGFSAHQHHHHGGAVAQGGLEAAALLLESSGGSRTPPPPSGGSYCDDLVASCANNAFAFSAGQELGSLIAPLTLQPPPPPSAPQQGCFANAATFYRCHQQQSGGGGGGSCCPPPGPPASPPFSFQPLRRLSESPVFDAPPSPPDSLSDRESYLSGSLSSGSLSGSESPGLDSGRRLPIFSRLSISDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X320</accession>
    <name>A0A6I9X320_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CMT1A duplicated region transcript 1 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106538312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013908237.1">
        <property type="nucleotide sequence ID" value="XM_014052762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X320"/>
    <dbReference type="GeneID" id="106538312"/>
    <dbReference type="KEGG" id="tsr:106538312"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd22136">
        <property type="entry name" value="F-box_FBXW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF7">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING PROTEIN 10B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="275"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="453"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="495"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="574"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013908237.1"/>
        </source>
    </evidence>
    <sequence length="657" mass="75147" checksum="7573CB8D3538C220" modified="2020-10-07" version="1">MKTPSVAEDEATHEYKLKYAPDLRCEKQTDLVPVCYRCKSCILSWKIFSTREWFVRASQATQRTFVMGIINRFESYDLLKYSWNLLQAIDAKDFNYSRSSVSSTFRASSALDRAMDPQKLAQSMADLWRWFAGTCFWTKANYILLLLQMCDSKLLLMAATLIRILIGEHENAMPENVEKEEYEKRSKSDMRPLLPMRSTLSQVGETQFLSKASSVFGNIHVQKIWSKQSDNIFKKALSELESDTNYFAVLTLHATTPPASPQLSPNKSKDFIRYLPVHLSKSILRMLDMKTLSRCASVSPHWNFLVKQVKCDVVANYVLRSQISFFQGLCPKGAISTYAKIVKVAIPRITANGDIVLTKTKKQFKDYDQQQITSQSEEIEYMQKAYHGQETDTIKLEERNVFCSGYNVRILINSLDSNRVIHSTAGRLLAFGSLDRKVHFLDIEKIKNVPPVFTGHAGSIRALYLHEERGFLLSGSYDLSIRMWNIRKGTCMKIFLGHKGNITCLDVCKFRFVSASKDCTAKLWDMRTARCIKTFNHKAHVWAAKMNEVYIVSSCDKGLVKVWHAESYILIKVLEGHRGAVKCLSFDEWHLVTGSSDGYILAWSMVGKHKRCLMAFRHPKEVLSLAFVYLRVISGCADGKIRIFNFFSGICLRVMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3T4</accession>
    <name>A0A6I9X3T4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Upstream-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106538857</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the grh/CP2 family. CP2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908961.1">
        <property type="nucleotide sequence ID" value="XM_014053486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3T4"/>
    <dbReference type="GeneID" id="106538857"/>
    <dbReference type="KEGG" id="tsr:106538857"/>
    <dbReference type="OrthoDB" id="9996779at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000036">
        <property type="entry name" value="upstream-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF12">
        <property type="entry name" value="GRH_CP2 DB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25416">
        <property type="entry name" value="GRHL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="11">
        <location>
            <begin position="50"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="228"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013908961.1"/>
        </source>
    </evidence>
    <sequence length="464" mass="52644" checksum="8C4034DEE3B06B46" modified="2020-10-07" version="1">MLFWHSQPDHYWPGPGEMYPCPGNNIIREPLSLPFLKQEEPNISTSAEQHCPSFQYILCAVTSPAVKQHEETLTYLNQGQSYEIRMLCNPKIGEFAEGRKLLKSVIRVVFHDRRLQYTEHQQLEGWRWNRPGDRILDIDIPMSVGILEPHMPSTLLNTVEFLWDPTKRTSVFLQVHCISTEFTLRKNGGEKGVPFRIQADTFKPNEKGEHMEHLHSASCLIKVFKPKGADRKQKTDREKIEKQSLQEREKYQPSYDSTVLTECSPWPEVSSNLNSPPSTPGLVSPHPFKLQTPERVCSSPSYALDSSSEGMAETLNPSASILETQQWLLKNRFSNYSRAFASYTGTDLLKLSRNDLVQICGMADGIRLSNALKARTVRPRLILYVSKELEASGNNAPPNPASVIYQELYLEELTATELTNKLAELLSVPANQVQQVSKQGPTGIHILVNDQVWCRLILRMSLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4K1</accession>
    <name>A0A6I9X4K1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger and SCAN domain-containing protein 16-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106538717</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908782.1">
        <property type="nucleotide sequence ID" value="XM_014053307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4K1"/>
    <dbReference type="GeneID" id="106538717"/>
    <dbReference type="KEGG" id="tsr:106538717"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="9">
        <location>
            <begin position="171"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013908782.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="52712" checksum="55BCAEFE209B71F0" modified="2020-10-07" version="1">MADGEVPRTGFSLQLQAALEKWMQPGFKRQDLGRAAPKAGEELGGDRKSCRGLEGTEQLVKQEPDLLSQQWEAQWQEFLKMTESQQPWGVVSLVQEEPSPWNDAKAFLASFEQVAEACQWPREEWASRLLPALRGEAERAYCNLGSAEREDYGKVKAAILQRDSLSREKLRQHFRRFCYQEAEGPRGAYSRLQALCHQWLKVEHHSKEQILELLILEQFLAILPPEIQSWVRGNSPETCTQAVSLAEDFLQMHVEGHQEQEVLKEVRDAAEDCGKGPLFREANHEGNSGDAGEGSRGVDKMEKYGLENSEPETSHEMLSGRTEEPVAHFGDQENLSQSQDRLLWPHKFLPKEMGDSAVPCRGSYRELGQTKTPMVKKYTASSVIARSFYHKSNFAKQKKIHPGEKTYSCLNCGRRFSRRSNLKRHEGIHTGERPHECLECGKKFTRKSYLLKHSTVHMYGAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6G5</accession>
    <name>A0A6I9X6G5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Potassium voltage-gated channel subfamily B member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Voltage-gated potassium channel subunit Kv2.1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="37 38" type="primary">KCNB1</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="37 38" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="37 38">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="29">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Synapse</location>
            <location evidence="30">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909846.1">
        <property type="nucleotide sequence ID" value="XM_014054371.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909847.1">
        <property type="nucleotide sequence ID" value="XM_014054372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6G5"/>
    <dbReference type="GeneID" id="106539562"/>
    <dbReference type="KEGG" id="tsr:106539562"/>
    <dbReference type="CTD" id="3745"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18412">
        <property type="entry name" value="BTB_POZ_KCNB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000034">
        <property type="entry name" value="Potassium voltage-gated channel subfamily B member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000018">
        <property type="entry name" value="Potassium voltage-gated channel subfamily B member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000010">
        <property type="entry name" value="Potassium voltage-gated channel subfamily B member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003973">
        <property type="entry name" value="K_chnl_volt-dep_Kv2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004350">
        <property type="entry name" value="K_chnl_volt-dep_Kv2.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF63">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03521">
        <property type="entry name" value="Kv2channel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01514">
        <property type="entry name" value="KV21CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01495">
        <property type="entry name" value="SHABCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="26" id="KW-0966">Cell projection</keyword>
    <keyword evidence="32" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="27" id="KW-0407">Ion channel</keyword>
    <keyword evidence="23" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="24 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="20" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="13" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0770">Synapse</keyword>
    <keyword evidence="15" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="16 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="21 34" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <keyword evidence="18" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="19" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="35">
        <location>
            <begin position="31"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="472"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="695"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="761"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="848"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="32">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="504"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="537"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="717"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="771"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00031679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="RefSeq" id="XP_013909846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="RefSeq" id="XP_013909847.1"/>
        </source>
    </evidence>
    <sequence length="869" mass="97959" checksum="D34BADD46F06EFEF" modified="2020-10-07" version="1">MPAGMNKHGSRSTTSLPPDPMEIVRSKACSRRVKLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHESLMDICDDYNLEENEYFFDRHPGAFTSILNFYRTGKLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETMREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQDTDEYGNPSDNPQLAHVEAVCIAWFTMEYVLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEEDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNLKDAFPRSIELMDIVVEKKGENAGKKDKVQDNHLSPSKWKWAKRSISETSSNKSLETKEQGSPEKARSSSSPQHLNVQQLEDMYNKMSKAQSQPNLNLKETAQPSKQKEELEMEAIPGPMVPLLTTRADGIIDMRSMSSIDSYISCMTEFPEAGRYSHSPLTTLTSKGGKLPLQDPYRLEGSGSKFMEIKPNLESRHCSTFFIESPKSSIKLINPLKLRSLKVNFMEEDAVGSAASNVLRIYPDPIKNRGATGAATDSSGYSDRSLMSPETSVYTTASARTPTKSPEKQAPIVMNFHDAGIHKFIDADTDDEGQLLYDSSPPKASSGHTSPKYSISQRGYIRQRDNVCKTEKDRLATAALSSTHKLLGQNCLYSMENITGRTHGNQETIKLDNHISPEVHILPGGGGGGRGHSNKHDQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9L6</accession>
    <name>A0A6I9X9L6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450 3A</fullName>
            <ecNumber evidence="13">1.14.14.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106538078</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17149"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:142491"/>
            <dbReference type="EC" id="1.14.14.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013907948.1">
        <property type="nucleotide sequence ID" value="XM_014052473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9L6"/>
    <dbReference type="GeneID" id="106538078"/>
    <dbReference type="KEGG" id="tsr:106538078"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000003">
        <property type="entry name" value="cytochrome P450 3A12-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008072">
        <property type="entry name" value="Cyt_P450_E_CYP3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002402">
        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050705">
        <property type="entry name" value="Cytochrome_P450_3A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24302">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24302:SF42">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 3 SUBFAMILY A MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00464">
        <property type="entry name" value="EP450II"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01689">
        <property type="entry name" value="EP450IICYP3A"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0492">Microsome</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602402-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013907948.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="58664" checksum="A3F0F2C9E7434B5A" modified="2020-10-07" version="1">MGDPVLGWALETWVLILLFLTLLMLYGIWPYNFFKKLGIPGPRPLPFIGTFHKYRHGILKFDQTCFEKYGKIWGIFDGRQPVMGILDPALIKTILVKEFYTYFTNRRDFGLNGNLDTAMTVVVDEQWKRIRSALSPTFTSGRLKEMMPIINHYNEILEKNIQKKVENDETIDMKLIFSAYSLDVVTSTSFSVNIDSLNHPDDPMVVHMKKFLRFSFLSPVLILAVLFPFVTPLLDKLNFTIFPKSSIDFFFGVLKKIKEDRQKKDHTNRVDFLQLMMDAQAAANSSKDPNAEKALTDKEILAQSIFFIFGGYETIGTSLSFMSYCLAVHPDVQEKLYQEINETFPNRAPPTYDALHQMEYLDMVVNETFRLYPPGTRLERICKKTVEVHGVTIPKGTVVLIPAYVLHRIPEYWPEPEEYRPERFSKENKESLEPYAFLPFGAGPRNCIAMRFALLVLKMGLVVALQRFRFQTCKETPIPMELDDRGFLKTKKPIMLKLVPRVVVKDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAE6</accession>
    <name>A0A6I9XAE6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Zinc finger and SCAN domain-containing protein 16-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106538295</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908218.1">
        <property type="nucleotide sequence ID" value="XM_014052743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAE6"/>
    <dbReference type="GeneID" id="106538295"/>
    <dbReference type="KEGG" id="tsr:106538295"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001818">
        <property type="entry name" value="GDNF-inducible zinc finger protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000508">
        <property type="entry name" value="Myeloid zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000642">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="16">
        <location>
            <begin position="158"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="254"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="360"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013908218.1"/>
        </source>
    </evidence>
    <sequence length="579" mass="65111" checksum="ECFCEB52902B6290" modified="2020-10-07" version="1">MQRLQGGGGGQKEVKMELKGGGKEKKTRAVQKGLQATHAGIGAELQFQKLLKPIKQEPEDGLQQRWETQWQKFPKTVQAPYFPERCPQLLQPPPEESPKGFQASFPFLKGTAETKLRSREEVFSQKACRPCEKVPRSSVEVKDEIWGEDMDSLEEQSQHFHRFRYQEAEGPRNVCEQLWKLCHQWLKPERHTKEQILELVVLEQLLTILPEDIQGWVREGRPQTCAQAVTLAEDFLLNLQRRQQDLEPYEEVIVNSPKAELDPSDAEEPGNSTEVERGSDDEPPLFSDGERCRSHHSEGMEHGELTRMSSKNIGGQTNLHPCFASGGENEELNLHPENAQLVEFGDISPERGRGTFLQVPEVAGRPGSQERPRHPQGNSARKLPRKNVTCREIDSDRSPSQERVPEHLPQKAATGCVRKSGPRADLAQPAKAPSYECSYCGKRWPCQSQLRRHLKIHTGERPHKCTDCGKSFSNSSNLSQHKRVHTGERPYSCKDCGKSYRRRASLLQHERETCRKGNPLTAPAVGYILLGNCTLLCMKKSVQSGKNRVIAPKGAEVPLAAQPLNLAMGYSQEKSQGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAJ0</accession>
    <name>A0A6I9XAJ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CGG triplet repeat-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CGGBP1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908253.1">
        <property type="nucleotide sequence ID" value="XM_014052778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAJ0"/>
    <dbReference type="GeneID" id="106538325"/>
    <dbReference type="KEGG" id="tsr:106538325"/>
    <dbReference type="CTD" id="8545"/>
    <dbReference type="OrthoDB" id="9434539at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033375">
        <property type="entry name" value="Cggbp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32344">
        <property type="entry name" value="U1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32344:SF1">
        <property type="entry name" value="U1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="1">
        <location>
            <begin position="38"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013908253.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="18808" checksum="E53115FFECC9CD91" modified="2020-10-07" version="1">MERFDVKPPPSRSRSKTALYVTPQDRVTEFGSELYEDGGKLYCSFCNVVLNHVRKSAINDHLKSKTHTKRKGEFEEQSVRKKPRTLTASLQCNSSTQTEKPSVVQDFVKMFLEASIPLEKADHPAVRAFLSRHVKNGNSIPKADQLRKIYLSDGFTNQLIKSEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD40</accession>
    <name>A0A6I9XD40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ECSIT</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ECSIT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911762.1">
        <property type="nucleotide sequence ID" value="XM_014056287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD40"/>
    <dbReference type="GeneID" id="106540968"/>
    <dbReference type="KEGG" id="tsr:106540968"/>
    <dbReference type="CTD" id="51295"/>
    <dbReference type="OrthoDB" id="10064298at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007178">
        <property type="term" value="P:cell surface receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029342">
        <property type="entry name" value="ECIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010418">
        <property type="entry name" value="ECSIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046448">
        <property type="entry name" value="ECSIT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13113">
        <property type="entry name" value="ECSIT EVOLUTIONARILY CONSERVED SIGNALING INTERMEDIATE IN TOLL PATHWAYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13113:SF1">
        <property type="entry name" value="EVOLUTIONARILY CONSERVED SIGNALING INTERMEDIATE IN TOLL PATHWAY, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14784">
        <property type="entry name" value="ECSIT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06239">
        <property type="entry name" value="ECSIT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01284">
        <property type="entry name" value="ECSIT_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ECSIT C-terminal" evidence="12">
        <location>
            <begin position="273"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911762.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="48980" checksum="5C7AF5B743C4B06E" modified="2020-10-07" version="1">MKCLRALLVAGQLSARRSFFPKKNLIQSQRSSYFQNSSLVPVRSIQRSTVLSTSDFAVKKHEKPDKESAKQSLVKIEDIFETVSEHEKTSATFQEAVEIFCKQDIRRTGHVEFIYAALKKMPEFGVEKDVNVYNKILDVFPKKVFIPQNVFQKMLKHYPRQQDCAVVLLEQMEHFGVMPNLETRFLLLEIFGNKSYPIRKYQRIMYWFPKFKHINPYPVPTPLPEDPIELATLSLQRIAADLDSKVTVYQTSHSEVLDSGKEINHPHIVGTQSPDQQSLLASHNTEKPIFLEGPFRLWLKAKCVYYYVLRAKLSEEHMVEVIDPERNFYYPMHLDLDLERDVLDDFEFYTDKVKEGPVFAMCMVGAEDKATLAKWIVGLQETNPILHDITVIFRLTHSPQELQCNQETEGENGGTQAKFKQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEC9</accession>
    <name>A0A6I9XEC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Growth/differentiation factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GDF2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909468.1">
        <property type="nucleotide sequence ID" value="XM_014053993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEC9"/>
    <dbReference type="GeneID" id="106539241"/>
    <dbReference type="KEGG" id="tsr:106539241"/>
    <dbReference type="CTD" id="2658"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19400">
        <property type="entry name" value="TGF_beta_BMP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF157">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026963463" evidence="9">
        <location>
            <begin position="25"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="10">
        <location>
            <begin position="308"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909468.1"/>
        </source>
    </evidence>
    <sequence length="419" mass="47573" checksum="BB23F11655543DD9" modified="2020-10-07" version="1">MLYFGAWTLLYIILIVLQSGTFMGNQHSLPTKENSHKSSGEPGNTEGEAYFNFKAFLENLKADFLRNLNLSAIPSQEKIKEEPPQFMIDLYNRYTQDKSSSPIANIVRSFRPEDISSLSSLEINQKYILLFNVSIPCHEEVTKAELRIHIACQKEAECFPSVHGNVVIYDMHEGDNWEEAEGVRNFLASREIQGCGWVTFDVSSTVKKWVKTEKTRTVNRLETAVKSYDPSGFNCEHLNISVAPDSNHLPSLIVFSNDHGKRVKANHTELQEMVAHEQEDVLKSLVKGHAAHRRNSWPVHGTRHSLSRSKRSIELNHCRKTALHVNFKEIGWNWIIAPPDYEAFQCKGGCFYPLTDNVTPTKHAIVQTLVHYKNPKKAVKACCVPTKLEAISMLYKDEAGTPTLKYEYEGMKVAECGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFK2</accession>
    <name>A0A6I9XFK2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein DENND6A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DENND6A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DENND6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912657.1">
        <property type="nucleotide sequence ID" value="XM_014057182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFK2"/>
    <dbReference type="GeneID" id="106541669"/>
    <dbReference type="KEGG" id="tsr:106541669"/>
    <dbReference type="CTD" id="201627"/>
    <dbReference type="OrthoDB" id="10265409at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018307">
        <property type="entry name" value="ABL9/DENND6_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024224">
        <property type="entry name" value="DENND6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677:SF1">
        <property type="entry name" value="PROTEIN DENND6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09794">
        <property type="entry name" value="Avl9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="47"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912657.1"/>
        </source>
    </evidence>
    <sequence length="590" mass="67479" checksum="5B7BF224914F8DA5" modified="2020-10-07" version="1">MSLQKPAEGGSGGLADSPGAEEAEDGSPSPSLPPPVLPWDRFSAWLHCVCVVGFDLELGQAVEVIYPQHSKLTDKEKTNICYLSFPDSNSGCLGNTQFCFRFRQSSSRKLSWHCLLDQLDRDLPIYLKRDPAYFSGYVYFRQVQDKTLKRGYFQKSLVLISKLPYIHLFNTVLEQIAPEYFEKNEPFLEAACSDIDRWPLPVPGKTLYLPIMGIVIKVRIPTCHDTPGTTQVLQLTHQADSQISIALPSVYEVDLFRCFLPVFFHIQMLWELVLLGEPLVVMAQSPSESSETVLALVSCISPLKYYSDFRPYFTIHDSEFKDYTTRTQTPPSVILGVTNPFFAKTLQHWPHIIRIGDIKVSGEVPKQVKVKKLKNLKTLDSKPGVYTSYKSYLNKDEEIIKQLQKGVQQKRPAEAQNIILRRYFLELTQSFIIPLERYVASLMPLQKSISPWKSPPQLKPFSKEEFMKTLEKTGPQLTSRLKGDWIGLYRHFLKSYNFDGWFRTRRKEMTRKLEALHLEALCNEDLLFWSQKHTEVETVDLVLKLKTKLTDGENLPVKPGTIEKLKKHIDSIILAQPEDLQGILTKTGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH24</accession>
    <name>A0A6I9XH24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">2-oxoisovalerate dehydrogenase subunit beta, mitochondrial</fullName>
            <ecNumber evidence="3">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Branched-chain alpha-keto acid dehydrogenase E1 component beta chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BCKDHB</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Together with BCKDHA forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:13458"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterotetramer of 2 alpha/BCKDHA and 2 beta chains/BCKDHB that forms the branched-chain alpha-keto acid decarboxylase (E1) component of the BCKD complex. The branched-chain alpha-ketoacid dehydrogenase is a large complex composed of three major building blocks E1, E2 and E3. It is organized around E2, a 24-meric cubic core composed of DBT, to which are associated 6 to 12 copies of E1, and approximately 6 copies of the dehydrogenase E3, a DLD dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013913411.1">
        <property type="nucleotide sequence ID" value="XM_014057936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH24"/>
    <dbReference type="GeneID" id="106542266"/>
    <dbReference type="KEGG" id="tsr:106542266"/>
    <dbReference type="CTD" id="594"/>
    <dbReference type="OrthoDB" id="878at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007584">
        <property type="term" value="P:response to nutrient"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000004">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="12">
        <location>
            <begin position="50"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913411.1"/>
        </source>
    </evidence>
    <sequence length="371" mass="40756" checksum="EF7D2A2FB8A7BA97" modified="2020-10-07" version="1">MALAGLALRRAAVSSLRLRLATGSTPRRRAAHFAFQPDPEPTQYGQTQKMNLFQSITSALDNALSRDPTAVIFGEDVSFGGVFRCTVGLRDKFGKDRVFNTPLCEQGIVGFGIGVAVTGATAIAEIQFADYIYPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPESFFAHCPGLKIVIPRGPIEAKGLLLSCIEDKNPCIFFEPKIMYRAAVEQVPVEPYTIPLSQAEVLQDGSDVTLVAWGTQVHVIKEVAVMAQEKLGVSCEIIDLKTILPWDAETVCKSVAKTGRLVITHEAPVTGGFASEISSTVQEECFLNLEAPITRVCGYDTPFPHIYEPFYIPDKWKCYDAVRKMINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHA6</accession>
    <name>A0A6I9XHA6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein numb homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NUMB</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in the process of neurogenesis.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910403.1">
        <property type="nucleotide sequence ID" value="XM_014054928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHA6"/>
    <dbReference type="GeneID" id="106539955"/>
    <dbReference type="KEGG" id="tsr:106539955"/>
    <dbReference type="CTD" id="8650"/>
    <dbReference type="OrthoDB" id="10070446at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050769">
        <property type="term" value="P:positive regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01268">
        <property type="entry name" value="PTB_Numb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000031">
        <property type="entry name" value="protein numb isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016698">
        <property type="entry name" value="Numb/numb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010449">
        <property type="entry name" value="Numb_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368">
        <property type="entry name" value="NUMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368:SF5">
        <property type="entry name" value="PROTEIN NUMB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06311">
        <property type="entry name" value="NumbF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017607">
        <property type="entry name" value="Numb/numb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="5">
        <location>
            <begin position="37"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="224"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="398"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="630"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="442"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="636"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR017607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013910403.1"/>
        </source>
    </evidence>
    <sequence length="657" mass="71641" checksum="06EFD69E6CF2B7CD" modified="2020-10-07" version="1">MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKSERKFFKGFFGKSGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEVMRQIQDIKVETDVKTTASSVVPGNRAPSPGSPTSPTAEVATSAEKESSNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQLSLRINELPSTVQRKTDFPIKNSVPESEGEVDSISSLCSQITNTFSMPTEDPFSSAPMTKPVTVVAPQSPAFQVNGIASAFHVFADKPAQTTIASTAIPLRESNPWAQSPAAPAVAKTGAGASPSGADWSADSGEASPNLFQASHRRTPSEADRWLEEVSKTVRSQQPQVQPQPPAPQPLLQPPAVVSQAAPAFPVSTFIAPPPVPIGVVQPMQPTFIQAQPYAVANGMPYSAPSVTMIGITPSQMVANVFGTAGHSQVSHAHQSPSLIKQQTFPQYETNSATASPFFKPPAQQLNGSAAFNGADPAKWPTEDKHVLLPGAGQQVDPFEAQWAALESKSKQRTNPSPTNPFSSELQKTFEIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHZ2</accession>
    <name>A0A6I9XHZ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 22 member 7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC22A7</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913706.1">
        <property type="nucleotide sequence ID" value="XM_014058231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHZ2"/>
    <dbReference type="GeneID" id="106542484"/>
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    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000023">
        <property type="entry name" value="Solute carrier family 22 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="87"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913706.1"/>
        </source>
    </evidence>
    <sequence length="547" mass="61121" checksum="4F115F660218C138" modified="2020-10-07" version="1">MNFEELLGEVGGFGQFQILILLLLCIPRVLLPLHFLLHNFLAAIPEHHCAIPHQQQFANLTKEEVLIINIPRDFNGAFSSCEMFSEPQFHLLLNSSLELTNVSVQSCQYGWTYDQSQFISTIVTQWDLVCEHKSLNQATATLFFFGVMFGAVIFGDLSDRFGRKRMLLVSYLGTLIFGVLSSASVSYSMFAVTRTLTGISICGLSIIVIPLGMEWVDIQHRTISAVITSLFWSFGNMLLALIAYLVRDWHWLLLTTSLPCIVGIISTWWLSESARWLLIKGKLKEAHRHLKRCAKMNRKKEFAHKINPENLLKTAAVISQKSDGNYSYISLFRTPILRRISFCLAAVWFGAAFSYYIMSMDISGFGLGMYLTQFIFGLIEIPAKLLVFVMVNRVGRRQSQAWALILSGLSIGINIIVPTSQGTLRSVIATIGKGFSEAAFTIVFLYSSELYPTVLRQSGQGYCSFMARLGACVAPLVFLLDSIWKPLPQTASFAVAVLCGSSAFFLPETRGIRLPETIDDIEKQSSKGKKHLHGDAPEVAPLQLSHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIH1</accession>
    <name>A0A6I9XIH1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-tocopherol transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TTPA</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Monomer and homotetramer. Phosphatidylinositol 4,5-bisphosphate binding induces the formation of homotetramers. Phosphatidylinositol 3,4-bisphosphate is less efficient in inducing tetramerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915564.1">
        <property type="nucleotide sequence ID" value="XM_014060089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIH1"/>
    <dbReference type="GeneID" id="106543966"/>
    <dbReference type="KEGG" id="tsr:106543966"/>
    <dbReference type="CTD" id="7274"/>
    <dbReference type="OrthoDB" id="440711at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120013">
        <property type="term" value="F:lipid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008431">
        <property type="term" value="F:vitamin E binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042360">
        <property type="term" value="P:vitamin E metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051180">
        <property type="term" value="P:vitamin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.20:FF:000003">
        <property type="entry name" value="Alpha-tocopherol transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000002">
        <property type="entry name" value="Alpha-tocopherol transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF225">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="8">
        <location>
            <begin position="87"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013915564.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="31867" checksum="8CF1299B369BFA29" modified="2020-10-07" version="1">MDRDQPISPPGNLNDLPDDSPQVRAAVAALRWRAVEENLEVRQSDLSDACLIRFLRCRDFDSDLAWKVFKNYHKWRRDCPEIIANLHPSSSLSLLQTGYIGILKERDPCGSRVVIYRIARWDPKIFTAYDLFRVSIMLSELLAREPDTQRNGVKAIFDLQGWKFAHAFQVTPAVTKKITFALTDGFPLKVRGIHLIREPIIFYHVFNLIKLLLPEKIKARIHLHGNNFAHGLQKHFPVSLLPEEFNGEAGSFEEFSKYLIDFLMESINYLQSISLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIN8</accession>
    <name>A0A6I9XIN8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RING finger protein 122</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RNF122</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May induce necrosis and apoptosis. May play a role in cell viability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910788.1">
        <property type="nucleotide sequence ID" value="XM_014055313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIN8"/>
    <dbReference type="GeneID" id="106540254"/>
    <dbReference type="KEGG" id="tsr:106540254"/>
    <dbReference type="CTD" id="79845"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000423">
        <property type="entry name" value="Ring finger protein 122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013910788.1"/>
        </source>
    </evidence>
    <sequence length="103" mass="11754" checksum="7BC16219F3CD09C2" modified="2020-10-07" version="1">LIFCCYFISKLRHQAQSERFGYKEVVLKGDARKLNLHGQTCAVCLEDFKVKDELGVLPCQHAFHRKCLVKWLEVRCVCPMCNKPIGGPSEPHQNIGILLDELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLI0</accession>
    <name>A0A6I9XLI0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ankyrin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106544832</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916699.1">
        <property type="nucleotide sequence ID" value="XM_014061224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLI0"/>
    <dbReference type="GeneID" id="106544832"/>
    <dbReference type="KEGG" id="tsr:106544832"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000010">
        <property type="entry name" value="Ankyrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2660:FF:000002">
        <property type="entry name" value="Ankyrin-1 isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040745">
        <property type="entry name" value="Ankyrin_UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF71">
        <property type="entry name" value="ANKYRIN 1, ERYTHROCYTIC A ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17809">
        <property type="entry name" value="UPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Death" evidence="6">
        <location>
            <begin position="160"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="343"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916699.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="46517" checksum="525555B3C902A8AD" modified="2020-10-07" version="1">MNDGREGRLRCYCMTDDKVDKTLEQHENFTEVARSRDIEVAEGMPLHMELSGNLLPIKKAAQQRNFLFQSFHENRLIIPVKVRDSSREASGSLSFLRKPMKYEELQHVLCHLNVTMPPCSKTLGSEERRRTLTPLALRERYSLLSDGTLGSLNSTEKDKTNMKLAVIAEHLGLSWAELARELQFGVDDINRIRVENPNSLLEQSLTLLNLWTSRGGQSANMESLYAALRNIDRSEIINMLEGSGRQSHSLKSDKRYRHRDYSGSPSQINGYASLQDELLSPASMHYALPSPLCADQYWNEVAILEAIPMAASEQDALLEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDATSEGRFTGQTLEDVHGADPLGSMDLVEEDTVDSDAMNGLMDMLEQEEGQRPTESPSGHHRVAHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM20</accession>
    <name>A0A6I9XM20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 9A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TTC9B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TTC9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911878.1">
        <property type="nucleotide sequence ID" value="XM_014056403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM20"/>
    <dbReference type="GeneID" id="106541076"/>
    <dbReference type="KEGG" id="tsr:106541076"/>
    <dbReference type="CTD" id="148014"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000264">
        <property type="entry name" value="Tetratricopeptide repeat protein 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039663">
        <property type="entry name" value="AIP/AIPL1/TTC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242:SF13">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911878.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="23364" checksum="68A9041F2FF8B140" modified="2020-10-07" version="1">MEERSPYQKSCPEAGGTSLVPGTGGGACRAGCAAAAELEAKLQRAVEFKAEGHRCYKQKKLREAIGKYHRALLQLKGLEPPAAEGGGEAAAAKGSSLVAWRHLSAEQREQADNTEMECYDSLTACLLQSELVNYERVREYCLKVLAKEKGNFKATYRAGIAFYHLGDCDKALLYLKEAKCRQPTDTNVLRYIQLTEMKISRCHQREKEVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMI5</accession>
    <name>A0A6I9XMI5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium channel subfamily K member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">KCNK5</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912013.1">
        <property type="nucleotide sequence ID" value="XM_014056538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMI5"/>
    <dbReference type="GeneID" id="106541180"/>
    <dbReference type="KEGG" id="tsr:106541180"/>
    <dbReference type="CTD" id="8645"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000077">
        <property type="entry name" value="Potassium channel subfamily K member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF241">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="81"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="172"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="409"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013912013.1"/>
        </source>
    </evidence>
    <sequence length="484" mass="55290" checksum="9FFA20F2E975E671" modified="2020-10-07" version="1">MVDRGPLLTSAIIFYLSIGAAIFEVLEHPYLKNAAEGYKQKKSELLKEFPCLSQEGLDKILRVVSEAAGQGVSIDGNDTFNYWNWPNAVIFAATVITTIGYGNVAPKTPAGRLFCIFYGLFGVPLCLTWISALGKFFAGRAKRLGQFLTKRGVSLRKAQITCTAIFIFWGILVHLVLPPLVFMVTEEWDYIEGLYFSFITITTIGFGDYVAGVNPNMEYHSLYRYFVELWIYLGLAWLSLFVNWKVSMFVEVHKAIKKRRKKRKESFENHSPTQKVLQMSNKKDIKIFSFLSKKEESYNDLIKQIGKKALKKSNDKILNSEQTKPTLQNTNKDLKVMYTRSNVFDYDNVSLRLQNYHMMKHLTDKRLGDSPTEGNVFVNQLDRISEEEGEAWDSRDYRPLIFENANITFVNEGDDEEEDLSDDEETSKSSVDDNLAQEPQSPQKLMKFSSSDESTFTSNEMEISVPYEKLMNDYNTSDTANAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XN81</accession>
    <name>A0A6I9XN81_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lamin-L(III)-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915356.1">
        <property type="nucleotide sequence ID" value="XM_014059881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XN81"/>
    <dbReference type="GeneID" id="106543802"/>
    <dbReference type="KEGG" id="tsr:106543802"/>
    <dbReference type="OrthoDB" id="102442at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LTD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915356.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12375" checksum="59CE769F815691A3" modified="2020-10-07" version="1">MVRKQHRNETDMMYEFPAQFILQAGHVVTIWAKSLGLKPSPSTLIWESQRACRLGETLGIVLLDGDGNEIADGMITYLERGEGEGEVEDAAEQTKLGIHSQQNETRSCPIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNZ7</accession>
    <name>A0A6I9XNZ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein PALS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Protein associated with Lin-7 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">MPP5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915697.1">
        <property type="nucleotide sequence ID" value="XM_014060222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNZ7"/>
    <dbReference type="GeneID" id="106544072"/>
    <dbReference type="KEGG" id="tsr:106544072"/>
    <dbReference type="CTD" id="64398"/>
    <dbReference type="OrthoDB" id="43580at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048699">
        <property type="term" value="P:generation of neurons"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06798">
        <property type="entry name" value="PDZ_MPP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12036">
        <property type="entry name" value="SH3_MPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000105">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000088">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000469">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015145">
        <property type="entry name" value="L27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035601">
        <property type="entry name" value="MPP5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09060">
        <property type="entry name" value="L27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
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    </dbReference>
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        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="L27" evidence="20">
        <location>
            <begin position="132"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="20">
        <location>
            <begin position="191"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="268"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="357"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="18">
        <location>
            <begin position="491"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013915697.1"/>
        </source>
    </evidence>
    <sequence length="687" mass="78474" checksum="D441CDC16E9A4A35" modified="2020-10-07" version="1">MSDKPYSFSHRTMTTSHMNGHVTEDSDSETRIMDLAPLEESQKHREMAVDCPGDLGNRMMPLRRSAQLERIRQQQEDLRRRREEDGKKQELDLNSSMRLKKLAQIPPKTGIDNPMFDTGEGVILESPHCAVKVLEVDELLCSLKHVQHTLIDPQSQEEIALILQLVQNTDFQNAFKIHNAVTVHMNKASPPYPLISNAQELAQEVQNVLRPSHQKDGLELNSLLNAPHVQALLLAHDKVAEQEMQPESVADEKIYENVGVYGGETVKIVRIEKARDIPLGATVRNEMDSVIISRIVKGGAAEKSGLLHEGDEVLEINGTEIRGKDVNEVFDLLADMHGTLTFVLIPSQQNKPPPTKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYRDGDEENQPLAGLIPGKSFQQQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIVLIGPQNCGQNELWQRLMNNEADRFASAVPHTTRNRRDNEVAARDYHFVSRQVFETDITAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLNLHTQSLKSLRNSDLKPYIIFIAPPSQERLRALLAKEGKTPKPEELREIIEKTREMEQNNGHYFDTAIVNSDIDKAYQELLRLINKLDTEPQWVPSSWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPH6</accession>
    <name>A0A6I9XPH6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PITH domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PITHD1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915912.1">
        <property type="nucleotide sequence ID" value="XM_014060437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPH6"/>
    <dbReference type="GeneID" id="106544223"/>
    <dbReference type="KEGG" id="tsr:106544223"/>
    <dbReference type="CTD" id="57095"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045654">
        <property type="term" value="P:positive regulation of megakaryocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.470:FF:000002">
        <property type="entry name" value="PITH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="4">
        <location>
            <begin position="20"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00074054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013915912.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="24265" checksum="A3B5C864C3155BFD" modified="2020-10-07" version="1">MAHGHSHGGGSCCHSDDHEEPPERRGQAWGLYLRIDRERLECLNERVEGSGARVFRAWEDRGDKQKFVESDDDEELLFNIPFTGNVKLKGIIVMGEDDNTHPSEMRLFKNIPHMSFADTAREPDQIFSLNHDFLGELEYPTKIARFSNVYHLSIHFSKNFGAETTKIFYIGLRGEWTEPHRHEVTICNYEAAANPADHKLDQITPQTHFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQZ1</accession>
    <name>A0A6I9XQZ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amyloid beta A4 precursor protein-binding family B member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106544447</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916191.1">
        <property type="nucleotide sequence ID" value="XM_014060716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQZ1"/>
    <dbReference type="GeneID" id="106544447"/>
    <dbReference type="KEGG" id="tsr:106544447"/>
    <dbReference type="OrthoDB" id="5969782at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01272">
        <property type="entry name" value="PTB1_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01271">
        <property type="entry name" value="PTB2_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000019">
        <property type="entry name" value="Amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000034">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039576">
        <property type="entry name" value="APBB1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058:SF11">
        <property type="entry name" value="AMYLOID BETA PRECURSOR PROTEIN BINDING FAMILY B MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="42"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="161"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916191.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="36967" checksum="C7A0C4FFD5ED941F" modified="2020-10-07" version="1">MGEEDLAPGKSSVAVNNCIRQLSYCKNDIRDTVGIWGEGKDMYLILENDMLNLVDPMDRSVLHSQPIVSIRVWGVGRDNGRDFAYVARDRETRILKCHVFRCDTPAKAIATSLHEICSKIMAERKNAKAVACNSLQERSNVALEVPLQVDFPTPKTELVQKFHVQYLGMLPVTKPVGMDMLNGTIENLMESSNREEWTPVTMNVADATVTVISDGNEEEIVVECRVRFLSFMGVGKDIHTFAFIMDAGNQHFECHVFWCEPNAGNVSEAVQAACMLRYQKCLVARPLSQKVRPPPPPADSMTRRVTTNVKRGVLSLIDTLKQKRPVTDMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR96</accession>
    <name>A0A6I9XR96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAM pointed domain-containing Ets transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Prostate epithelium-specific Ets transcription factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Prostate-derived Ets factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SPDEF</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907230.1">
        <property type="nucleotide sequence ID" value="XM_014051755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR96"/>
    <dbReference type="GeneID" id="106537576"/>
    <dbReference type="KEGG" id="tsr:106537576"/>
    <dbReference type="CTD" id="25803"/>
    <dbReference type="OrthoDB" id="10057999at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08532">
        <property type="entry name" value="SAM_PNT-PDEF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000050">
        <property type="entry name" value="SAM pointed domain containing ETS transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000220">
        <property type="entry name" value="SAM pointed domain-containing Ets transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF182">
        <property type="entry name" value="SAM POINTED DOMAIN-CONTAINING ETS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02198">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PNT" evidence="16">
        <location>
            <begin position="174"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="15">
        <location>
            <begin position="292"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013907230.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="43335" checksum="5C1466EC07C4E25C" modified="2020-10-07" version="1">MNIENFFGGRLGDQLRIENEILERHKDLEENLHRVMGSAGQGVVALPHGRVTLQDPVLFSGLEKLPLAQAPDSPSWQSLDSPSPPATPEQTLPTYCLQYFNMLYPDDSNWVPKSLGETQQHNHQGSRTEVHKEPEQCPIIDSQGLSLTSEMDYQASLHLEEHSLEQVQTMVVGEVLKDIETACKLLNIAADPADWSPGNVQKWILWTEHQYRLPQIGKSFQDLSGKELCDMTEEGFRQRSPSCGDVLHAHLDIWKSAAWMKEKTAPGELHYCGNEENWTDSEVDSSCSGQPIHLWQFLKELLLKPHNYGRFIRWLNKEKGIFKIEDSAQVARLWGIRKNRPAMNYDKLSRSIRQYYKKGIIRKPDISQRLVYQFVHPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSM5</accession>
    <name>A0A6I9XSM5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rho GTPase-activating protein 23-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106542468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913683.1">
        <property type="nucleotide sequence ID" value="XM_014058208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSM5"/>
    <dbReference type="GeneID" id="106542468"/>
    <dbReference type="KEGG" id="tsr:106542468"/>
    <dbReference type="OrthoDB" id="6281275at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175:SF5">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913683.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="41848" checksum="51BBB17DD1FFBFFD" modified="2020-10-07" version="1">MLAISFISAVNRKRKKRREAKRFGSSTDDDSEHESAVKRDEEEEEEVAQEEEREAERLGNSSRAPVPDVQDVFQSRAKLEAEQVERKVAPRGSPELALDARSIVSGYSTLSTIDRSLCSEVQSVAESRGEEADDERSEFSHVETDTENSFVPKQPGMLPKGAGVAVVEEGGAAASEKISHNRTSFSSHHLMQCDTLARRKLLRPRHNSESSSKGSTDVPSSSSPGSQESLQPVGAPEIHTRLSLTEQLRLRLRGSADDMLAVRLRKPHSPETRRKKNSWRRHTVVVPGGLKDLNYEWKEQHVLGTTEAGPLDSSNRGALRDNKDSGLSSLESTKARSSSITPGNMTVPNQQSEPQHRKGSSEGRSAPRRTASLRFHQCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSS5</accession>
    <name>A0A6I9XSS5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Saccharopine dehydrogenase-like oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SCCPDH</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907785.1">
        <property type="nucleotide sequence ID" value="XM_014052310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSS5"/>
    <dbReference type="GeneID" id="106537981"/>
    <dbReference type="KEGG" id="tsr:106537981"/>
    <dbReference type="CTD" id="51097"/>
    <dbReference type="OrthoDB" id="10268090at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000178">
        <property type="entry name" value="Saccharopine dehydrogenase-like oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="4">
        <location>
            <begin position="10"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013907785.1"/>
        </source>
    </evidence>
    <sequence length="428" mass="46883" checksum="A95D6EC4CACF8CE7" modified="2020-10-07" version="1">MATERPFQLVVFGASGFTGQYVVQEVARVAAEDELRGSLRWAVAGRSREKLQDVVEKAAAKLGKTELKSEIGIILCDVSDPSSLASMAKQTNIVLNCVGPYRFFGEPVVKACVENGTNCIDISGEPQFLEGMYLHYNSKAEEKGVYIVGSCGFDSIPADMGVLFTKDSLKGTLTAIESFLNVKSGPEGACIHDGTWKSAVYGLADQENLKKIRKQIGYKQLPVFGAKLKRRGTVFYSNEFKQYSIPFMGSDASVVKRTQRYLHSHLQETPVQYGAYTTVGGLTSVIKLMFAGLLFLMLTKFNFGRKLLLKYPEFFSGGHFTKDGPTEKQMEESSFEMTFLGEGYSTGKNSLEGKPDVKICTQVKGPEAGYVATPIAMVQAAVALLKDKNSLPKKGGVYSPGAVFYKTKLVERLNKHGIEFSVISKSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTC6</accession>
    <name>A0A6I9XTC6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Splicing factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106542670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913943.1">
        <property type="nucleotide sequence ID" value="XM_014058468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTC6"/>
    <dbReference type="GeneID" id="106542670"/>
    <dbReference type="KEGG" id="tsr:106542670"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Splicing factor 1 helix-hairpin" evidence="2">
        <location>
            <begin position="159"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913943.1"/>
        </source>
    </evidence>
    <sequence length="549" mass="57910" checksum="38DDE30D12F27E3F" modified="2020-10-07" version="1">MATGANATPLGKLHPPPPPGKAGYPLPPPGLVMQGPPPPPPPPGPAQAQPQPAIPSLMAAAGFPFAALPPPPPPPPPPPPPQQPQPPPQQPQPPPPPPPQAQQPQPAQYGQYRFPSPPPPQQPMGLEPQPPPQHLHLLQQDDGGQAGAGNSDYPNKKRKRSRWNQDTMEQKTIIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKYNRPPWMNSGPSENRPFHNMHGGSGSSGSSSSGGPAGPSGPHNFPHPMSSMGGHGGHPMQHNPSGPPPWMQPHNPPMNQGPHPHGHPGPHHMDQYLGNAPVGSGAYRLQGKGMMLPPMGMMPPPPPPPSGQPPPPPSGPLPPWQQQQQQQQPPPPPPSSSTPLPWQQNTTTTTSAGTGGIPPWQQQQGAGAATSTGGPQMQGSPSMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPMDPSNFVTMMGVGVPALPPFGMPPAPPPPPPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTW6</accession>
    <name>A0A6I9XTW6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Tyrosine-protein phosphatase non-receptor type 23</fullName>
            <ecNumber evidence="6">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">PTPN23</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes. May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013919534.1">
        <property type="nucleotide sequence ID" value="XM_014064059.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547032"/>
    <dbReference type="KEGG" id="tsr:106547032"/>
    <dbReference type="CTD" id="25930"/>
    <dbReference type="OrthoDB" id="10266451at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09239">
        <property type="entry name" value="BRO1_HD-PTP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14539">
        <property type="entry name" value="PTP-N23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.280:FF:000002">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000061">
        <property type="entry name" value="tyrosine-protein phosphatase non-receptor type 23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
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        <property type="entry name" value="Y_phosphatase"/>
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        <property type="entry name" value="PRTYPHPHTASE"/>
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    </dbReference>
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        <property type="entry name" value="PTPc"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
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    </dbReference>
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    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
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    </dbReference>
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        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="19" id="KW-0969">Cilium</keyword>
    <keyword evidence="13" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="23" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="20" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="16" id="KW-0904">Protein phosphatase</keyword>
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    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
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            <begin position="8"/>
            <end position="394"/>
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            <begin position="1266"/>
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    <sequence length="1754" mass="191001" checksum="E078E7FE5F6A5FD0" modified="2020-10-07" version="1">MEAVPRMPMIWLDLKEAAEFSFQSAVKKFVLKNYGENPEDYNEELKKLEQLRQNAVNVPRDFEGCSILRKYLGQLHYLQSRIPMGAEQEASVPITWTEIFSGKAVTHEDIKYEQACVLYNLGALHSMLGAMDKRVSEEGMKVSCTHFQCAAGAFTYLRDHFPHSYSVDMSHQILNLNINLMLGQAQECLLEKSMLDNRKSFLVARISAQVVDYYKEACRALENSDTASLLGKIQKDWKKLVQMKIYYFAAVAHLHMGKQAEEQQKYGERVTYFQSALDKLNEAIRLAKGQPETVQEALRFTMDVIGGKYNSSKKDNDFIYHEAVPALDTLQSIKGASLVKALPVNPTDPAVTGPDIFAKLVPMAAHEASSLYSEEKAKLLRDVMAKIDAKNEILEQFMDSLQLDPETVDNLEVYDHIPPVLMEKCAALSVRPETVKNLVQSMQVLSGVFTDVEASLKDIKDLLEEDESEEQKLQETLGKAHPSPPDPAVPSTGGVAEVSKEWAKYLEVHQKASFTNTELHKAMNLHITNLRLLSGPLEGVKATLPSPSLSEVLTRFRLHLAHWRWTEIFSGKAVTHEDIKYEQACVLYNLGALHSMLGAMDKRVSEEGQAQECLLEKSMLDNRKSFLVARISAQPNLRAQPHRFHLCSSSNREMKKKAPSRPTTSKPVLQKKASEAELACEPLGLASISLADLPEELRSLPPEVLAAHLAQLPADALAALRSEPPLPTAGIRNPVGEAFAGVLPLRPPFDPGQFPVTGPPPRPYQPARMSTASGQAPFPVLAPRSSTASPLPNSQVYPPGVATGRVPAHVPHQPVYPGPPLPPSRTSPQHVPMSRPPPGSVYGVGPMAPSSGVLPAGPPTPLAMRPPIPVSGHFVAPHFPPHTQGVRPATTTVDSLQAPISSCTAPQGMSGSLPQWAGPPSQGGFVGGPPGSAPYPFAYVPAGTAPFRPPFSGPQAQPGFPSQGPIHPPSDRPACLQTMSNMLPSQQQQQQQPMPHFLPQTQQPQPAFPQPYPSQPSGRPGLPNPVATHPASQGHLPHQLGPQHYQLPPRDPLPPHSGNLAFSAPAPRSMMGPVQQVPLHRHGAAAPLAFTPTSAQIPGVPVGLAGPQPPPPVSHPPLQHVPPAPSSVGGAVPPPAPSPRPSMTLPPPCLLPSQMPPSSLTPGPPSLPHAASEVPFQRPSSSTDDLLSSSPESQHGGPKAPVGQPLLQPTKADAKDGLKPKAVQLIENDPYEKPERLWRLLSELERFQQGVSRLEKTPAPGTVSELDNIWKELQDTQEKDARQLSIAIARCYTMKNRHQDIMPYDRNRVILQSGKDDYINASRIEDLSCYCPTIIATQAPLVGTASDFWLMIYEQKVSLVVMLVSEQEMEKQKVVRYFPTERGQPMVHGPITLVLTSLKATPTHLERMITLQFCDQSLKRTVIHLQFSSWPELGLPDSKGNLLRFIQDVHSHYLHQRPLHTPIVVHCSSGVGRTGAFCLLYAAMQEVEAGNSIPDLVQLVKRIRQQRKHMLQEKVHLRFCYEAVLKHAVQVLQRHGVMAAPSASKPASSGTQKVYLQQDPQDLVLGGDVPISSIQATIAKLSIRPLPGGAEPPPNGRGLEVGPSLPPFDAALLPASSAATPRNFPEPGCVESTPGFSPPVTPAPALPETMAASTVEKSSSMEEERSEEKVESVPRVEPLAPTQALPTSSSSSSSLELLASLTPEAFTMDNALKGRHKVSKQSFLQPQNGEGLRGALPSDDPLSKLDPLWTLNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUY0</accession>
    <name>A0A6I9XUY0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106545757</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917887.1">
        <property type="nucleotide sequence ID" value="XM_014062412.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tsr:106545757"/>
    <dbReference type="OrthoDB" id="9829410at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15418">
        <property type="entry name" value="7tmA_OR9G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0552">Olfaction</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="198"/>
            <end position="227"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="42"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013917887.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="35021" checksum="F4C4BBA63D6870F2" modified="2020-10-07" version="1">MEVEDNRTLITDFVVVGFTTDPFLRTILFVVFLALYILTLIGNLGLITLIYLDSRLHTPMYFFVGSLSFLDVWYSTVYTPRILYDCLSKNNHISLAGCAAQFFFSNVVGGSECFLLAFMAYDRFVAICNPLLYAIAMPKKLCIQLVVGAYTVGFANAIVHTGNTFRLHFCGSNIINHFFCDVPPLMKMACDDKRVYELILTVVIGFTLLSATAVIAKSYIGIVAAIIRIRSAAGRRKAFSNCSAHLVSVSLYYGTMLITYSAPNSHHTADWDKVNALFYTVINPLVNPLIYSLRNKDVKAAFKKVWGRFTAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVK4</accession>
    <name>A0A6I9XVK4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sorting nexin-14-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106545912</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918112.1">
        <property type="nucleotide sequence ID" value="XM_014062637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVK4"/>
    <dbReference type="GeneID" id="106545912"/>
    <dbReference type="KEGG" id="tsr:106545912"/>
    <dbReference type="OrthoDB" id="5957963at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08722">
        <property type="entry name" value="RGS_SNX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037892">
        <property type="entry name" value="SNX14_RGS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF44">
        <property type="entry name" value="SORTING NEXIN-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="1">
        <location>
            <begin position="210"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918112.1"/>
        </source>
    </evidence>
    <sequence length="474" mass="55027" checksum="0347B0AF346B2DFB" modified="2020-10-07" version="1">MFELVLENFIYPWYREITDEESPVDELRVTLRFFASVLLRRIYKVNIPTVVTKRMLKAAMKHIEVIAKARQKVKNTEYLQQAALEEYGPSLHVALRSRRDELNYLRKLTELLFSYILLPKATDCGTFTLLIREILSGSVFLPSMDYLADPDTVNHLILIFIDDSPPEIATEPASPLVPFLQKFAETRNTKSSALHFELKEFEGKQDLFFLFMSFLKKEGTVHVLQFYLELEQFNNRILQAELSDAEKMSLHEEVKKIYETYCLDESIDKIKFDPFIVEEIQSIAEGPYEEVVKLQQMRCFFEAYEQVFSLLENVFIPRFCHTYEYFHHLFQEVESPTRNSKSNKSLHRRGESSGIRSISSKIKGVFKSTTMEGAMLPDYLLAEGEDDFNFQYESASWEISGVKIHIIGDASVWEEESCPKERGVGPEVKVIRMSQQFVESPTPLGLSMTPKMMKNTRDAVQVWITLIEMLQEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWH3</accession>
    <name>A0A6I9XWH3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CUB and sushi domain-containing protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106545919</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918121.1">
        <property type="nucleotide sequence ID" value="XM_014062646.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106545919"/>
    <dbReference type="KEGG" id="tsr:106545919"/>
    <dbReference type="OrthoDB" id="5804959at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000001">
        <property type="entry name" value="CUB and sushi domain-containing protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000002">
        <property type="entry name" value="CUB and Sushi multiple domains 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052129">
        <property type="entry name" value="Spermadhesin-Link_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46908:SF8">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46908">
        <property type="entry name" value="CUBILIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="4">
        <location>
            <begin position="29"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="3">
        <location>
            <begin position="92"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="4">
        <location>
            <begin position="203"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="3">
        <location>
            <begin position="264"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013918121.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="37364" checksum="215AF41CCACEED8F" modified="2020-10-07" version="1">MLKCCDXNALAQWNDTLPTCVAVPRTSSTQCSSVPEPRFGKRIGNEFAVGSLVLFECNPGYILYGSIAIRCDTVPNALAQWNDTLPTCVVPCGGILTKRKGTILSPGYPEPYDNNLNCVWKITVPEGAGIQVQVISFATEHNWDSLDFYDGADNNAPRLGSYSGTTIPPLLNSTSNNLYLNFQSDISVSAAGFHLEYTAIGLDSCPEPQIPNSGIKIGDRYMVGDVVSFQCDHGYSLQGHSHITCMPGPVRRWNYPIPICLAQCGGSMSDFSGVILSPGFPGNYPSSLDCTWTIKLPVGFGVHLQFVNFSTETIHDYLEVRSGSPDVSTAIGRLSGTQLPSSLFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX64</accession>
    <name>A0A6I9XX64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA polymerase</fullName>
            <ecNumber evidence="21">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">POLD1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="21"/>
    <dbReference type="RefSeq" id="XP_013920804.1">
        <property type="nucleotide sequence ID" value="XM_014065329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XX64"/>
    <dbReference type="GeneID" id="106548020"/>
    <dbReference type="KEGG" id="tsr:106548020"/>
    <dbReference type="CTD" id="5424"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000003">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000351">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0228">DNA excision</keyword>
    <keyword evidence="7 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="18 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15 21" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16 21" id="KW-0408">Iron</keyword>
    <keyword evidence="17 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="26">
        <location>
            <begin position="142"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="24">
        <location>
            <begin position="241"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="23">
        <location>
            <begin position="551"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="25">
        <location>
            <begin position="1022"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013920804.1"/>
        </source>
    </evidence>
    <sequence length="1114" mass="125837" checksum="B4BDD04AB8902DBB" modified="2020-10-07" version="1">MENKRKVSVRRISIGDFTPSQQKRSRGGDWDEDGPSQFEEELAFLDEVEAEVAVEMKEAQLSPDAIPLGNLFSSIHNPKWQRPLPPKINPNEEALCFQQVELDYYVGSHIPGFPGCTQGSVPVLRMFGVTAAGNSVCCHIHGFAPYFYVPAPPGFQAENLAEFQRELNTAVIRDMRSNKDGLNQVVLAVEICKKQNMYGFHGPHLLPFLKITMALPRLIAPAKRLLEQGLRCGTLGTHNFQAFEANIDFEIRFMVDRDIVGCNWIELHAGKYRLRQEESAGESDKENPRKVSLCQLEADVAWMDFISHPPEGEWQRIAPLRVLSFDIECAGRKGIFPEPEKDPVIQIANMVLRQGEKDPFVRNVFTLQNCAPIVGSQVLCFSKEAELLKTWAEFIRTVDPDIITGYNIQNFDLPYLLTRAQTLKVIPFPFLGRILSRKSVIRDSSFQSKQMGRRENKVINMEGRAQIDLLQVLLRDYKLRSYTLNAVSYHFLQEQKEDVQHSIITDLQNGSDQSRRRLAVYCLKDAYLPLRLLGKLMCVINYMEMARVTGVPLSYLLARGQQIKVVSQLLRQAMKQNLVMPVVKTEGGEDYAGATVIEPLKGYYDVPISTLDFSSLYPSIMMAHNLCYTTLLQQGAVECYGLSPEQFIRTPTGDHFVKSSVRKGLLPEILENLLAARKRAKLELKQETDPFKRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTIANGYKADAKVIYGDTDSVMCRLGVESVAESMEIGREAAAWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSNPDMHDKMDCKGIETVRRDNCPLVANLINTCLQKLLIDRDPSGAVTHAKEVISDLLCNRVDISQLVITKELTRTADEYAGRQAHVELAERMRRRDPGSAPNLGDRVPYVIISAAKGVAAYMKSEDPIYVLENNLPIDTQYYLEQQLAKPLLRIFEPILGEGKAQNVLLKGEHTRCKTVLTAKVGGLMAFATKRSTCIGCRAVLNHHGAVCKFCLAHQSELYQKEVTHLSCLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQELLISRFGPPTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXS2</accession>
    <name>A0A6I9XXS2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NFATC2-interacting protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NFATC2IP</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918967.1">
        <property type="nucleotide sequence ID" value="XM_014063492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXS2"/>
    <dbReference type="GeneID" id="106546591"/>
    <dbReference type="KEGG" id="tsr:106546591"/>
    <dbReference type="CTD" id="84901"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17078">
        <property type="entry name" value="Ubl_SLD1_NFATC2ip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052324">
        <property type="entry name" value="NFATC2-Int_DNA_Repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47187">
        <property type="entry name" value="NFATC2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47187:SF1">
        <property type="entry name" value="NFATC2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="210"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013918967.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="32025" checksum="77D39434BA9F32AD" modified="2020-10-07" version="1">MEEPVTLSSDSEPEGAPKKPLPKRRRILTESVAIPVYSTQVENSLKLLQNSMKLPDEIKERCKNLSFSSEEDEEEEKPVPLKQLRPRPSRPFDQSLRNLNNSLSAAKKSLQDQRFDEDDVILVDAFEPQELLLKVRCRADLHKVCILMTEPLRRVVDHMAQILKVHPNRIILLHHDSELSADATPAKLDLGVASIIDCIVESNKQANESGNLLLRVQGKDRSSVMDITVHKGEPLETLMNNYKQGQGQGKGRLVFYFDGQRLTETQTPEQLGMESGDVIEVWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXV4</accession>
    <name>A0A6I9XXV4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MINOS1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion inner membrane</location>
            <topology evidence="2 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919002.1">
        <property type="nucleotide sequence ID" value="XM_014063527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXV4"/>
    <dbReference type="GeneID" id="106546617"/>
    <dbReference type="KEGG" id="tsr:106546617"/>
    <dbReference type="CTD" id="440574"/>
    <dbReference type="OrthoDB" id="1916310at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013919002.1"/>
        </source>
    </evidence>
    <sequence length="61" mass="6762" checksum="25F7807989D15FB3" modified="2020-10-07" version="1">MASEGDLGRKWDRCLADSAVKLGKTWPLAFGSGMGLGMAYSNCQHDFQAPYLLHGRFIKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYY3</accession>
    <name>A0A6I9XYY3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein atonal homolog 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ATOH7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909695.1">
        <property type="nucleotide sequence ID" value="XM_014054220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYY3"/>
    <dbReference type="GeneID" id="106539428"/>
    <dbReference type="KEGG" id="tsr:106539428"/>
    <dbReference type="CTD" id="220202"/>
    <dbReference type="OrthoDB" id="6161578at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003407">
        <property type="term" value="P:neural retina development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19714">
        <property type="entry name" value="bHLH_TS_ATOH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000025">
        <property type="entry name" value="protein atonal homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032663">
        <property type="entry name" value="ATOH7_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF99">
        <property type="entry name" value="TRANSCRIPTION FACTOR ATOH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="40"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909695.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16182" checksum="150C053164567D06" modified="2020-10-07" version="1">MKPCKPNPKETPSADAGFPCSSESGCAAKGSSERLENAAKRRLAANARERRRMQGLNMAFDHLRKVVPQWGQDKKLSKYETLQMALSYIMALTRILAEAEKFSTQKGWINFHEEHFHACTGQKVAAETEPYAPTLFHFQPDHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ59</accession>
    <name>A0A6I9XZ59_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NLRC5</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013919627.1">
        <property type="nucleotide sequence ID" value="XM_014064152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ59"/>
    <dbReference type="GeneID" id="106547102"/>
    <dbReference type="KEGG" id="tsr:106547102"/>
    <dbReference type="CTD" id="84166"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045345">
        <property type="term" value="P:positive regulation of MHC class I biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045348">
        <property type="term" value="P:positive regulation of MHC class II biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041210">
        <property type="entry name" value="NLRC5_atypical_Card"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47189">
        <property type="entry name" value="MHC CLASS II TRANSACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47189:SF1">
        <property type="entry name" value="MHC CLASS II TRANSACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18461">
        <property type="entry name" value="Atypical_Card"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="5">
        <location>
            <begin position="194"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013919627.1"/>
        </source>
    </evidence>
    <sequence length="1181" mass="133873" checksum="4FE6078989EB676D" modified="2020-10-07" version="1">MTMDGAQRQSIIRRIWPQLIDFLSDRSEWVLDKAERLLSQGELRLVHTEPDLKKKRLLVLDMVLRDVDANPEVFRTFIQSVCMECSPPFELEITLLSISEEDAPESYSQCILRLMRRKYGISHSAEAAKTKDQFSSFSQRFVNLVIHQSKTSKLKVGKEKMKEEPMAAYESKDHDIPMMNLSDLFKSAQMGITQVVLLLGKAGVGKSWLVHKICQQWAEEAFPQFKWIFFFEFKQLNLIKRKLTLQELLFDYFLQPEQPEMVFQFLLKNAHQTLVILDSLDEFMDNIDLPPISFDHYSNLLIPRSVSELVANLCHGKLLPGCTVLITSRPKQLPTLLVRSTVLSVEIWGFNHDKVAEYVSCFFQQAFQKEQALALLNENGKLLSMCYIPVLCHIICICLEHLFLEDSESVQLPQTRTQFYLRMLQTFLCKHQGGRTLNEVDMAQHQTILGDLCELAFKGLEEKKGVFDAGEVPEHVKDFSCRFGLLLTSEMKMGKDSTQAGYTFAHFSLQEFLAALFLLTSPTVDGKMLKENFFLRTKWTLKKETRMPFTENNYVFLSGLLSQECRQFLCSLAGRNEVRIQERQSIIRKILQKLAVANLTGPRIVELSHCVYETQDICLAHHIATKLSFTFRNVKLMPFDMVTLAFLINGGSHGVCLDFDGCFMELDYLDIWGSCEPIKSLSFKNRQYGSEFAGALSKTLPKIKLLTTFQLTGGNLKRPGFEDLVRVLPNCHQLEIINLQDNRLEEQDMIKLAEIFPAMGKLKRMEKTGSLFMLEQKFPPQVGKGAFAVHRTSHPLLPDAEGPTSFDALVHFPALQKLWLTNDRIPPTSIEYIAKILQRGFAIEDINFSNSDLSLDNLSILLDSLEGKYHLKSINFGSLNLDNATILKLFSRLSTMPLLKILGLNNNHLDSRMCSHLAEVLKTTLQIEEIDLSHNKIDDAGMKAIAMALPGMKNVKLINFSCNSITSAGGQWIVEGLIESERLEILKLSGNSIGNKTLGKLAPILPYKKHLKVLHLSRCQLGSEGLGHLAGILAKCPQMEEINLSENNIGDKGVMSLMTPWPQSSQLRKIDLKACHIRDCASKTLIVGLSCCPSLEEIGLSWNELGDETAKELAMVLPGMIRVKILDLDKNSITVYGASMLARQLFRCPGIQTIRLWNNSIPRDLEQQLANQEPRLHFALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZX9</accession>
    <name>A0A6I9XZX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-dependent kinase inhibitor 1B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cyclin-dependent kinase inhibitor p27</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">p27Kip1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CDKN1B</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920022.1">
        <property type="nucleotide sequence ID" value="XM_014064547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZX9"/>
    <dbReference type="GeneID" id="106547400"/>
    <dbReference type="KEGG" id="tsr:106547400"/>
    <dbReference type="CTD" id="1027"/>
    <dbReference type="OrthoDB" id="6373236at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265:SF9">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 19" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="17">
        <location>
            <begin position="93"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="36"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="147"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013920022.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="29051" checksum="21BC5F5F80CBAC81" modified="2020-10-07" version="1">MRGSGRQGCCALSAREALPRLGPAERPPAVAVVAAAFSSSPSGTPSPSPAAASAPWPAARRAMSKVRVSNGSPTLERMEGRPADGPKPSACRCLFGPVDRQELAREWQEQSRHLEEAGRRRWNFDFRREHPLEGRFEWQALDRAALPDFYSRPPRLGGARPKADARERRLELNGDRSPSRGLRCAQAPAEEQEPEPESQQQDGAPLTLSFGPRKRPAADDSSPQSKRANTIEEISEEHCGEPPMASSEEQTPKKKTTSSPKRCQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y020</accession>
    <name>A0A6I9Y020_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dihydropyrimidinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DPYS</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="PTM">
        <text evidence="2">Carbamylation allows a single lysine to coordinate two divalent metal cations.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919596.1">
        <property type="nucleotide sequence ID" value="XM_014064121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y020"/>
    <dbReference type="GeneID" id="106547085"/>
    <dbReference type="KEGG" id="tsr:106547085"/>
    <dbReference type="CTD" id="1807"/>
    <dbReference type="OrthoDB" id="10258955at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004157">
        <property type="term" value="F:dihydropyrimidinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006208">
        <property type="term" value="P:pyrimidine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000076">
        <property type="entry name" value="Dihydropyrimidinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF50">
        <property type="entry name" value="DIHYDROPYRIMIDINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="3">
        <location>
            <begin position="52"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="2">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611778-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013919596.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="40574" checksum="3D614A384CA06D28" modified="2020-10-07" version="1">MQKLLIRGGKVVNADFSQVADVYIEDGIVRAVGPNLQPSSDGIQVLDATHKLVLPGGIDTHTHMEFPFMGIPTKDDFYQGTKAALAGGTTMIMDFALAQKGCSLIEAYNQWRNRADPKVCCDYALHMVVSWCSEQVKEEMKLLTKEKGVNSFKMFMAYKDVYMIGDKELHTLFSQCKQIGAIAQVHAENGDLIAEGEKKMLALGITGPEGHEMARPEEVEAEATLRAITIANSVNCPLYIVHVMSKSAAKIVADARRQGKVVYGEPIAASLATDGTHYWNKDWPHAAAHVMAPPLRPDASTPDFLMNLLANGDLTLTGTDNCTYDICQKAVGKNDFTKIPYGVNGVEDRMSVIWEKGVVSEISIHSLPQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y176</accession>
    <name>A0A6I9Y176_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Myeloid leukemia factor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">MLF2</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MLF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920145.1">
        <property type="nucleotide sequence ID" value="XM_014064670.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920146.1">
        <property type="nucleotide sequence ID" value="XM_014064671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y176"/>
    <dbReference type="GeneID" id="106547477"/>
    <dbReference type="KEGG" id="tsr:106547477"/>
    <dbReference type="CTD" id="8079"/>
    <dbReference type="OrthoDB" id="8707547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019376">
        <property type="entry name" value="Myeloid_leukemia_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13105">
        <property type="entry name" value="MYELOID LEUKEMIA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10248">
        <property type="entry name" value="Mlf1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013920145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013920146.1"/>
        </source>
    </evidence>
    <sequence length="270" mass="30687" checksum="C22478582C4533DC" modified="2020-10-07" version="1">MACCAAVRKRIHIIVMVIKAHPAFEGIMFRFMRDGEPEDPMFMMDPLAIHQQHVNRVLSGGFGYSPFLSIGNGTVPGTRQVSRRMQPGTVSPFGMLGMAGGFMDMFGMMNDMIGNMEHMTSSTNCQTFTSSTVISYSNLGEGPKVYQETSETRSAPGGIRETRRTVRDSNSGLEQMSIGHHIRDRAHIMQRSRNHRTGDQEERQDYINLDENDAAAFDDEWRRETSRFRTQRGLDYRRHDGNTGRRIEGGRLAIQGPEDSPSRQSRRYDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y188</accession>
    <name>A0A6I9Y188_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylinositol-4,5-bisphosphate 3-kinase</fullName>
            <ecNumber evidence="3">2.7.1.153</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106544873</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:21293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.153" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013916748.1">
        <property type="nucleotide sequence ID" value="XM_014061273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y188"/>
    <dbReference type="GeneID" id="106544873"/>
    <dbReference type="KEGG" id="tsr:106544873"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="UniPathway" id="UPA00220"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046934">
        <property type="term" value="F:1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08399">
        <property type="entry name" value="C2_PI3K_class_I_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000087">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038904">
        <property type="entry name" value="BRAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003113">
        <property type="entry name" value="PI3K_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045580">
        <property type="entry name" value="PIK3CG_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21331">
        <property type="entry name" value="BRCA1-ASSOCIATED ATM ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21331:SF2">
        <property type="entry name" value="BRCA1-ASSOCIATED ATM ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00794">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19710">
        <property type="entry name" value="PIK3CG_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51544">
        <property type="entry name" value="PI3K_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51546">
        <property type="entry name" value="PI3K_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI3K-ABD" evidence="10">
        <location>
            <begin position="35"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K-RBD" evidence="12">
        <location>
            <begin position="210"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2 PI3K-type" evidence="13">
        <location>
            <begin position="350"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="11">
        <location>
            <begin position="537"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="809"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="826"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013916748.1"/>
        </source>
    </evidence>
    <sequence length="1008" mass="114132" checksum="0988B4505750558F" modified="2020-10-07" version="1">MELESSDQAVVMRDDNKRRRRRRMKPYCTANTLSSTELISIEFVFPTTSIVLDIPTNCTVEQMKGQVWMRAVEGHQTSDLFRKITPDQFLLLYQKKGQWYEIYDKHQIMQTLDCILYWKVLQKKVGKIHVVEKPNLSEEVQEFQNQLNYLIGYDVTDVSNVHDDELEFARRRLLTPRTIEVSCRDPKLYSMPVWTTSKPLPEYLLSKIVHNNIFLNIHRGTTSQKIKVAVDDTPDVILQSFFTKMAKKKSLMNIPEDHSEQDFVLRVSGRDEYIVGETPIKNFHWIRQCLKKGDEIHLVLDEPPDPNQDEVQKEEWPLVDDCTGVSGYHEQLTIHGKDHERVFTISLWDCNRKFRIKIIGMDIPVLPRNTDLTVFVEANIQHSQQLLSQRRTTAKPFTEEVLWNVWLEFDIKIKDLPKGALLTLQIHCGKTPAPSAKGNLHPHDSPSSDPKYKTQLLYYVNLLLIDHRSLLRTGDYVLHMWKIPGKGEEQGSINADKLTSATNPDKENSMAISIVLDKYCHPIALPKHKIPSDPQGDRARVEMPNQLRKQLEEIIATDLLNPLTPEDKELLWHFRYESIKHPKAYPKLLSSVKWGQQELVAETYQLLAKREAWDLSTLDVGLPIQLLDCNFSDETVRTMAVQKLEGLEDDDVLHYLLQLVQVCAGGARLLEILSSDSEGFPRRAAVGVFIDWLKEGHPEASPDPAEFVSRALQAADGDLDWEVKIRALELAGVFIGQTFDGLGLGEGSASAGSSLAKTPAPLADALRLFCQMELFGFLFRGLQDCDRPVALKACEVLLALKSVLCKHRSKSSPAGDAAGLKDIPMETPPPPPPLSLPAGEAAEKDCQDPERLLLILESTDLEGLRKALDVSSDHLERNPESLLQDILAAAGNAEENKLTRKSTVSRWFLRRHPESQFGQWVFLVGYSNFRDKISSLVGRREERKAGKEHGETGVQAITSSNHLVNFVGKILWTKLDSAKWVEIVSHPSAVMTSLLDTECPNCACACPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1J8</accession>
    <name>A0A6I9Y1J8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATPase family AAA domain-containing protein 2B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ATAD2B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920792.1">
        <property type="nucleotide sequence ID" value="XM_014065317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1J8"/>
    <dbReference type="GeneID" id="106548011"/>
    <dbReference type="KEGG" id="tsr:106548011"/>
    <dbReference type="CTD" id="54454"/>
    <dbReference type="OrthoDB" id="5421at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05528">
        <property type="entry name" value="Bromo_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000021">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000699">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF5">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="318"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="563"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="601"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="645"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="670"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="683"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013920792.1"/>
        </source>
    </evidence>
    <sequence length="793" mass="90094" checksum="D54601925E64045A" modified="2020-10-07" version="1">MQNIVPASQRAVTSSGHALSPIIRPLLERTFNEMLGVLHKVFPHAEFSQGDKREGASNLILDDSEDENVSSIFETSCQPGSPKKQLPTAVHKPYLHYTMSAYHQPTSYRPRLLLSGERDSGQTSHLAPALLHSLEKIAVHRLDLPALYSVSAKTPEESCAQIFREARRTLPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFKIQYEEVFYIQRPSKEDRHKFFQELVLNQASMPPPRRKHTAVSDMEVLPLALPPPSRQLSEAEKQRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKVPMDLSTIISKIDKHNYLSVKDFLIDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKTCEEIKEARKKRGLSVVAEQTNAHGSTVQKTENRAEEAFLNRQKNAMTVWHNSANKCAFRLRRKSRRRSQWGKGIIKKRKVNNLKKDEDDAKFADDENQGDDNKLLENGELEGSIDCIEENGEETGDLSLTNDESSCDVMDIHGEQRLDNGTVGKENSIPSTEESSNESLVVNNNVMVNVEEDFKDESGSQRDHLNGEASEGTPLLPCQYDKCEPSNVSLIVDMDTAKQGVTSDDHSPKKSEASNYSPEGEHVLQQSCSEISQCSNNEKKSSSTDMETKETESDKEGTLKNKKLRKIALTQVPEEQLDPVPPVVVDRVRLMNLLDLLVKKSDNLTVDQLERLYSLLNQCIYRHRKDYDKSQLVQEMERTVDMFETFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y206</accession>
    <name>A0A6I9Y206_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Brother of CDO</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">BOC</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917058.1">
        <property type="nucleotide sequence ID" value="XM_014061583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y206"/>
    <dbReference type="GeneID" id="106545121"/>
    <dbReference type="KEGG" id="tsr:106545121"/>
    <dbReference type="CTD" id="91653"/>
    <dbReference type="OrthoDB" id="9998697at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000655">
        <property type="entry name" value="brother of CDO isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000852">
        <property type="entry name" value="brother of CDO isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001263">
        <property type="entry name" value="brother of CDO isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000205">
        <property type="entry name" value="Cell adhesion associated, oncogene regulated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000327">
        <property type="entry name" value="Cell adhesion associated, oncogene regulated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000352">
        <property type="entry name" value="Cell adhesion molecule-related/down-regulated by oncogenes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF3">
        <property type="entry name" value="BROTHER OF CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16625">
        <property type="entry name" value="ISET-FN3_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026878211" description="Brother of CDO" evidence="14">
        <location>
            <begin position="25"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="848"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="32"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="115"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="230"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="319"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="469"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="601"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="705"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="418"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="559"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1064"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1084"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013917058.1"/>
        </source>
    </evidence>
    <sequence length="1096" mass="121095" checksum="54DCED7B52112BE0" modified="2020-10-07" version="1">MTMIPEGERLIIAIACLLLTSANCITNLGDSLHVSVQPDSIVQKHGGLVTLGCVVDPLGINVTWRLNGKELLQSDDVLGIHIDQRLLNIAALNNHTVGRYQCIARMSEGVVASVPATVTLANLKDFKYDGQHVIEVDEGNTAVIACDLPESHPKAQVRYSVKQEWLEASRDNYLIMPSGNLQIVNASQDDEGTYKCAAYNPVTQEVKTSVSSERLRVKRSTAEAARIIYPTEAQTIIITKGQRLILECVASGIPTPRITWTKDGSSISGYNKTRFLLSNLLIDTISEEDSGTYSCMADNGVRKAASSVILYNVQVFEPPEVSMELAQKIITWGQSAKFTCRVRGNPQPSVVWLRNAVPLFSSHRMQLSRKALRLSSVGPEDEGIYQCMAENEVGSAQAMIQLRVSQPEISQILLKGPVGDSAQSATSSTRFTNSEKMPKGKTVPLKSKPTASAASVQCTSKELVSPAEAPVILSSPRTTKTDGYELLWRPRHDNKTPILYYIVKYRKVMNFSDNWIIRDVPSSQYHLSLTKLDPGSLYEVEMAAYNCAGEGQTAMVTFRTGRRPKPDIVASKEQQFPKDDPGIGARSSNQSDNNRLSPPEAPDRPTISVASETSAYVTWIPRGNGGFPIQAFRVEYKKLKKLGDWMLARSDIPPSRLSVEIKDLEKGTSYKFRVRAWNILGESEPSAASKPYVVSGYSNRAYERPVRGPYITFTDAINETTIMLKWMYTPASNNNTPIHGFYIYYRPTDSDNDSDYKKDVVEGDRYWHSISHLQPETSYDIKMQCFNEGGESEFSNVMMCETKARKSSGLPGRLPPSTVSTIQHPPLNSGHTVLGAGAMMARSSDLSYLIVGVVLCSIVLIIVVFIPFCLWRAWSKQKQAIDLGFPGAGLLVPSCQYTMVPLRGVSTPQVNGLHYANGGNGHFPSATVGYSYTKSYDCNSDEAQQQNETNAFLQGRALQNGNNISEPQRLTSPGLEHSSFLCGLPDDSINQLLQQHDNCQHHHHHHEQLLSLCHTTPERKMGNSTMEDMRNPAFHKDSTCCIGLVPVKHTGGTDLCQKRRHVWPTNPEDVSSGESSEKYLNSLPLHNSSDTCSSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y291</accession>
    <name>A0A6I9Y291_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8 9">DDB1- and CUL4-associated factor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8 9 10" type="primary">DCAF8</name>
    </gene>
    <organism evidence="6 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8 9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat DCAF8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910912.1">
        <property type="nucleotide sequence ID" value="XM_014055437.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910913.1">
        <property type="nucleotide sequence ID" value="XM_014055438.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910914.1">
        <property type="nucleotide sequence ID" value="XM_014055439.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910915.1">
        <property type="nucleotide sequence ID" value="XM_014055440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y291"/>
    <dbReference type="GeneID" id="106540359"/>
    <dbReference type="KEGG" id="tsr:106540359"/>
    <dbReference type="CTD" id="50717"/>
    <dbReference type="OrthoDB" id="4869960at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000144">
        <property type="entry name" value="DDB1- and CUL4-associated factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF21">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="571"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013910912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013910913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013910914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013910915.1"/>
        </source>
    </evidence>
    <sequence length="611" mass="68625" checksum="C5A6D4C862386AE7" modified="2020-10-07" version="1">MSDAGNSTEGKTNIPNGSLSSSPEEMSGTTEGPETSSGVEVEASDLSLSLTGDEVGPSQTYTESSTEEKDSDSMEETGHYSINELNRTYDHSPSEEEEEEAEEEEEEEEEEQQQQQQEEAEVEAQRSRWRAHRKRPNQDRDSSDDERALEDWVASETMALPLPRWRALQALRGRQLGSSARFIYDACGARLFVQRFCLQYGLEGHGGCVNTLHFNQRGAWLASGSDDLKVVVWDWVRRRPVLEFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRIAELSATQCCKTTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDEYVRIYDQRKINENENNGVLKKFCPHHLVNSESKANITCLVYSHNGSELLASYNDEDIYLFNSSHSDGAEYIKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKASCQVVQFMPGDKGGVVNCLEPHPHLPVLATSGLDYDVKIWAPTAEMPTQLTGLKEVIKKNKRERDEDSLNHADMYESHLLWFLMHHFRPRRRHRRQRDAGTGVADSDSEDSLSSSDTLDEEEEGPDRVQCMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2Q4</accession>
    <name>A0A6I9Y2Q4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18 19 20" type="primary">OSBPL8</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917402.1">
        <property type="nucleotide sequence ID" value="XM_014061927.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917403.1">
        <property type="nucleotide sequence ID" value="XM_014061928.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917404.1">
        <property type="nucleotide sequence ID" value="XM_014061929.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917405.1">
        <property type="nucleotide sequence ID" value="XM_014061930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2Q4"/>
    <dbReference type="GeneID" id="106545391"/>
    <dbReference type="KEGG" id="tsr:106545391"/>
    <dbReference type="CTD" id="114882"/>
    <dbReference type="OrthoDB" id="10053431at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13286">
        <property type="entry name" value="PH_OPR5_ORP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000002">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000030">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000020">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF216">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="868"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="152"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="323"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="788"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013917402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013917403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013917404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013917405.1"/>
        </source>
    </evidence>
    <sequence length="887" mass="101288" checksum="3F19F567F7595DCF" modified="2020-10-07" version="1">MEIASSFEGDSDRASLQHHGEIKEWQGASTAVTSSDESHTSTPGKMSQRHGKEGYLTPTKELHQLSFSPGQGHGFDRGKDDASQNKDDSSQSMSKSKSESKIYNGSEKDIPASSPTTKLTKKESLKVQKKNYREEKKRATKELLSTITDPSVIVMADWLKIRGTLKSWTKLWCVLKPGVLLIYKTPKNGQWVGTVLLNACELIERPSKKDGFCFKLFHPLEQSIWAVKGPKGEAVGSITQPLPSSYLIIRAASESDGRCWMDALELALKCSSLLKRTMIREGKEHEVNLADSTHVSFYGLLRANNVHSTESFPLNDSEIERHHFKDSDMYSDKSDRENDHDRDESDNDGLVESDTDTSERQDDSYIDPEPDHIKETIYVEEDSEELGEAGEASQTEIVSEENKSLIWTLLKQVRPGMDLSKVVLPTFILETRSFLDKLSDYYYHADFLSEAALEENAYNRMKKVVKWYLSGFYKKPKGLKKPYNPILGETFRCLWIHPKTNSKTFYIAEQVSHHPPISAFYVSNRKDGFCLSVNILAKSKFYGNSLSAILDGEGRLMFLNRGEDYVMTMPYAHCKGILYGTMTLELGGTVSITCEKTGYSAVLEFKLKPFLGNNENVNQIMGKIKLGKEVLATLEGHWDSEVIIRDKKTDATETFWNPTPEIRQHRLRRYTVKFEEQGDFESEKLWQHVTRAIINKDQTEATQEKYILEEAQRKAAKERKALNEVWVCRLFEPDPLTGEWNYRYADTRPWDPLNDMIQFEKDGIVQTKVRHRTPMVTVAKIKRSAKSKKEECGFSSPEPDNPDSSGSEAQLKHNTRIRKKTVDLAELQSAIESIKLTQEDIRRDITALRTRMTSSLPPPDNNFFQFKHYVFILLMVLLQLIINYLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y468</accession>
    <name>A0A6I9Y468_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA carboxylase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106546266</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918578.1">
        <property type="nucleotide sequence ID" value="XM_014063103.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF1">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="4">
        <location>
            <begin position="39"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013918578.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18235" checksum="BF735AF1B3C2DE50" modified="2020-10-07" version="1">MSGMYLVKKGREQRKLDLHRDFTVASPAEFVTRFGGNCVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADQYVPVSGGTNNNNYANVELIVDISKRIPVQAVWAGWGHASENPKLPELLQKHGIAFLGRCLCVGTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4C6</accession>
    <name>A0A6I9Y4C6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IQ motif and SEC7 domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106546408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918753.1">
        <property type="nucleotide sequence ID" value="XM_014063278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4C6"/>
    <dbReference type="GeneID" id="106546408"/>
    <dbReference type="KEGG" id="tsr:106546408"/>
    <dbReference type="OrthoDB" id="8960626at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918753.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16388" checksum="A4EEAA3806C85FAD" modified="2020-10-07" version="1">MEAPAENPSKAAEYLKELNKIIETQQELLEKQKLRIDELEQQVAKMYHENARLQEEHRRHLATCRLVLLQQQQGALSAPPGKGQEISQHEKSESENSRSIRSINTLHQFCCPAPLYHTLLTLPATHSEYSSTCESKTILQRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4M4</accession>
    <name>A0A6I9Y4M4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 50B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM50B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919038.1">
        <property type="nucleotide sequence ID" value="XM_014063563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4M4"/>
    <dbReference type="GeneID" id="106546648"/>
    <dbReference type="KEGG" id="tsr:106546648"/>
    <dbReference type="CTD" id="757"/>
    <dbReference type="OrthoDB" id="268928at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013919038.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17803" checksum="A46F5F39EF35E936" modified="2020-10-07" version="1">MAGFLDNFRWPECECIDWGERRNAVASVVAGVLFFTGWWIMIDAAVAYPKPEEMNHAFHTCGVFSTLAFFMINAVSNAQVRGDSYGDGCLGRTGARIWLFIGFMLMFGSLIASIWILFGAYVTQNITVYPGLAVFFQNALIFFSTLIYKFGRTEELWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4U7</accession>
    <name>A0A6I9Y4U7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exportin-5-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106541604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912580.1">
        <property type="nucleotide sequence ID" value="XM_014057105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4U7"/>
    <dbReference type="GeneID" id="106541604"/>
    <dbReference type="KEGG" id="tsr:106541604"/>
    <dbReference type="OrthoDB" id="2215036at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013912580.1"/>
        </source>
    </evidence>
    <sequence length="279" mass="31700" checksum="41E1CC69F9414D52" modified="2020-10-07" version="1">MRLSQKWQVINQRSLVCDEDAVDDNPESQEMLEEQLVRLLTREVMDLIAVCCVSKKGAEQNNTVTADGDDEEMMSTEVTPSGSSELTELGKCLMKQENVCTALLITAFTSLSWKDTLSCQRTTTHLCWPLLKQVLSKNMMIPEAVIWFFTSVLKGLQVHGQHDGCMAALVHLAFQIYEALRPRYAELKTVMEQVPEIQKDSLEQFDSKVLNVTLQKAADKRRKDHFKRLIAGCIGKPLGEQFRKEVHIRNLPSLFKKHSSPTVICPVLYEQPFLHAYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5M8</accession>
    <name>A0A6I9Y5M8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Synaptotagmin-9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106547342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919948.1">
        <property type="nucleotide sequence ID" value="XM_014064473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5M8"/>
    <dbReference type="GeneID" id="106547342"/>
    <dbReference type="KEGG" id="tsr:106547342"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919948.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="26755" checksum="345368934EF31615" modified="2020-10-07" version="1">MPGGRDSSSSTSSIQPRGRSWKQQTSGGNGFTAAAAKHAGVSPVASLWAPDERRAAAEAARVSERGAPLLSQGCWGTMPRGREDEICRKALKLLVELCSEGTVENDRCLEFSYYLRDSGRPRPADADITVSLLSLVVTACGLALFGVSLFVSWKLCWVPWRERGLLGNKDKQESLNLTDTETNDQDYSEEFLGQPTTYPDSSMKISHTSPDIPLDAKAGTKENCIHNVRMHRQITEPTPSARSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6Q6</accession>
    <name>A0A6I9Y6Q6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106548115</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013920913.1">
        <property type="nucleotide sequence ID" value="XM_014065438.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106548115"/>
    <dbReference type="KEGG" id="tsr:106548115"/>
    <dbReference type="OrthoDB" id="9043430at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF12">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="12">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="13">
        <location>
            <begin position="643"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="897"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013920913.1"/>
        </source>
    </evidence>
    <sequence length="917" mass="100652" checksum="24C0CA6869F1385A" modified="2020-10-07" version="1">MAKLQNHYMLTPSRIARHYVFRQCVQKPAESISQFLQSLRVAAIQCDFGDLEDNLVEQFVCRVKDIRLRCCLLAHLEITLQQAVDEARAAKFSEQSTADMQHLQPLPPSLSPPPPPPLPPLCPPPAAQACLIDELVPLVEPDYEQVAQLRAQPRQPPPMPAVLTSSACVGCGGRHNRSACPFKGTICRRCGKKGHITQVCRASLPAPSFSSLLFLNRNRAASLRGLRGCTNLAEGSQDAKVDAELDKLLVQGVLEPIDHSRWETPIVVPVKPDGSGCTFAKLDMVQAYQQLPVDDDAASAQTIVTHRGAFRCHRLEFGVSMAPGIFQSLMKRLLHGLRDVVPYFDDVLIAGSSCSELIEMLRKVLLHFRACDASPYGLGAVLSHVLPNGSEAPVAVYSQTLSSAERNYSQIDKEALAAVSGIKKFHDYLYGRHFTLVMDHKPLLGLLTGDKLTPPILSPRMTRWTEFLAAYPYTLLYRPGKQLGHADALSRCPLPVSDQAPVPSLSVLSIASSGLPLTAADVAAHIKADPVLAHVCSWVLRGWPLGKVADSFRPFKARQDELSLHSGCLIWGDRVVITPILCSQVLSLLHXDHPGIACMKALACSYVWWPLLDAEIVAWVGRWSPCQLSHSTPPAAPAREWEALRGPWSQIHIDFAGPFHGQTFLIVVDAYSRWVELVLMTSTTAESTVRALRRLFAIHGLPDMVVSDNGLQFASTTFQEFVALQGIWHEPTTPYHLACNGRAEHAGRTGRWIRVETPRGSVKEAFPTETGVPSDPGAHNTAACQLLQRFQFEAFPTQMLQDFHLPVLSFNPSSSAQIHTTGPLPRQAPPIQMPPAGQHPRFTLKLIRPHSPVTTLVHHQSPPPGTVTPPKLLHPQGLAYLLSCAHLLGHPKMAPLGAPLPLGRSYTEQDSPLRRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAZ1</accession>
    <name>A0A6I9YAZ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106548424</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921265.1">
        <property type="nucleotide sequence ID" value="XM_014065790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAZ1"/>
    <dbReference type="GeneID" id="106548424"/>
    <dbReference type="KEGG" id="tsr:106548424"/>
    <dbReference type="OrthoDB" id="9017499at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000024">
        <property type="entry name" value="Vomeronasal 2, receptor 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="584"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="621"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="654"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="691"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="743"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="778"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="809"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="584"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921265.1"/>
        </source>
    </evidence>
    <sequence length="845" mass="95369" checksum="C3DDB04666F2B946" modified="2020-10-07" version="1">MCDISVAKCIISEPRNINHKYYQSGDVIIADICSQSYVFPNTLYFERHPSSEDLYELIYLTQTYQHVLAFLFAVKEINEDTQMLRNISLGFSIYNDYFSGSLTYLASLELLSTWDHFIPNYKCDARSTNVAVIGGPDSEACLLMATILSIYKIPQLGYGSSPMINDQSEQFHIQWMFPNVNQQYVGILQLLMLFSWTWIGVIYIQDDSSHGFMQNELPKLSAGGICFDFIKPFPNVYFKTGIQKMIEAWFKLYLLISDSTASVVFLHGKFHTTIFLRMFPRILELENVQMKRAKVWMLTAQMDFTSLPFQQSWDIDILHGSLAFATQSKELVGFQHFLQMRNPNVETRDGFIKDFWAQAFDCSFPTSLEEEKAWNHCTGKEKLEALPKSVFDMSITAPSYSVYNAVYAVAHALQYMTSLKFKHRTMFGKGQKKLLNQQLWQHSSNSSSMASYINLHRCILSRSELTLKIAIDEAHTSEMADQSMPDLRHFPPPTPPAFPVSTVHHQQAQPLSLCNEPCHKGHSRSKKEGKPFCCYDCLPCPRGKVSDQIDVDSCYQCPEDHYASQAQDRCILKEISFLSFQDPLGFILAIFAVLFSFITALVLTIFIHHKDTPIVKANNRGLSYTLLISLLLSFLCPLLFIGQPQRATCLLRQTAFGIIFSVAVSCVVAKTLTVVLAFMATRPGSKMRSWVGNRLAIFMVFSCSSIQTTLCTVWLLSSAPFPDLDMHSLPTEMVLECNEGSTAMFYAVLGFVGLLAMISFTVAFLGRNLPDSFNEAKFITLSMLLFCSVWISFVPAYQSTKGKYTNSSSSHVVVTSSAWICSIFIFFTGGTAAAEDADETPPPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB49</accession>
    <name>A0A6I9YB49_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable aminopeptidase NPEPL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NPEPL1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921327.1">
        <property type="nucleotide sequence ID" value="XM_014065852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB49"/>
    <dbReference type="GeneID" id="106548475"/>
    <dbReference type="KEGG" id="tsr:106548475"/>
    <dbReference type="CTD" id="79716"/>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000035">
        <property type="entry name" value="Probable aminopeptidase NPEPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10590">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041417">
        <property type="entry name" value="NPEPL1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF4">
        <property type="entry name" value="AMINOPEPTIDASE NPEPL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18295">
        <property type="entry name" value="Pdase_M17_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="5">
        <location>
            <begin position="340"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921327.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="56174" checksum="04AE868223666515" modified="2020-10-07" version="1">MASVRLEFQASAGEADPQSRPVLVLGQLQNLHRLPWAHLRGKLQPRVTEEIWQAALNTLNPNPTDSCPLYLSHATVAALPSRVSRHNSPSAAQFLTRVTRTCLPGGTKRCILMVCERSEVFASACAIARAFPLFTLRSSSTRRTEKKTVVVEFFLIGQNNGPVEVEMLKCLESATEGVRLAARIVDTPCNEMNTDKFLEEIRKVGKDLGIVPTIFQNEELKEKGFGGIYGVGKAAIHPPALAVLSHTPEGATQTIAWVGKGIVYDTGGLSIKGKTTMPGMKRDCGGAAAILGAFKATVKQGFKDNLHAVFCLAENSVGPNATRPDDIHVLYSGKTVEINNTDAEGRLVLADGVCYACKDLGADIILDMATLTGAQGIATGKYHAAVLTNNEEWEQACIKAGRNCGDLVHPLVYCPELHFSEFTSAVADMKNSVADRDNSPSSCAGLFIASHIGFDWPGVWVHIDIAAPVHAGERATGYGVALLLSLFGRASEDPLLNMVSPLGCSGDPLEDGMGRDCKRRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBR6</accession>
    <name>A0A6I9YBR6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">inositol-polyphosphate 5-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.56</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">INPP5A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type I family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.56" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013921568.1">
        <property type="nucleotide sequence ID" value="XM_014066093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBR6"/>
    <dbReference type="GeneID" id="106548666"/>
    <dbReference type="KEGG" id="tsr:106548666"/>
    <dbReference type="CTD" id="3632"/>
    <dbReference type="OrthoDB" id="5780965at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09092">
        <property type="entry name" value="INPP5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039737">
        <property type="entry name" value="INPP5A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12997:SF2">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE-5-PHOSPHATASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12997">
        <property type="entry name" value="TYPE I INOSITOL-1,4,5-TRISPHOSPHATE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013921568.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="48254" checksum="C6F4CAAF3B298EAA" modified="2020-10-07" version="1">MAMKAAAANTGVLLVTANVGSLFDDPENLQKNWLREFYQVVHAHKPHFMALHCQEFGGKNYEASMSHVDKFVKELLSSDAMKDYNRARVYLDENFKSQEHFTALGSFYFLHESLKNIYQFDFKAKKYKKVTGKEIYSDTLESTPMLEKEKFPQDYFPECKWSRKGFIRTRWCITDCAFDLVNIHLFHDASNLVAWETSPSVYSGIRHKALGYVLDRIIDQRFERVSYFIFGDFNFRLDSKSVVETLCAKATLQTIRAADTNEVVKLIFRESDNDRKVMLQLEKKLFEYFNQDVFRDNNGTALLEFDKELSVFKDKLYELDISFPPSYPYSEDCSQGMQYMNTRCPAWCDRILMSHSAKELVLKSENDERQVVYDHIGPNVCMGDHKPVFLSFRIAAGAGKRCLRHEKIFRESSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDN8</accession>
    <name>A0A6I9YDN8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proline-rich protein 22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PRR22</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922135.1">
        <property type="nucleotide sequence ID" value="XM_014066660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDN8"/>
    <dbReference type="GeneID" id="106549098"/>
    <dbReference type="KEGG" id="tsr:106549098"/>
    <dbReference type="CTD" id="163154"/>
    <dbReference type="OrthoDB" id="9941921at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031535">
        <property type="entry name" value="PRR22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37871">
        <property type="entry name" value="PROLINE-RICH PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37871:SF1">
        <property type="entry name" value="PROLINE-RICH PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15776">
        <property type="entry name" value="PRR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922135.1"/>
        </source>
    </evidence>
    <sequence length="448" mass="48709" checksum="C365E3806CC096F2" modified="2020-10-07" version="1">MQQPKVFYPQHETYASRPLDRADGQQQNRGFPAFVLPENLASVGTPSLYHPPSQDLCAAPPAGFHMAPCGCFFDPRIYRIEWATANFVQPSVYKLSGGPNPQNSYLLDSQKYLKGSVQPLPYAPAYQPVAGHPPFAMPFFKPEGSPGKLTDHVGFVANLAHGPPFVEVPHLPGESLMPVEEHKLLAVVPSSSLKEQQQQQQQHHSQVGPYQPLDPRAGLAYPVFKGFPAEEVDFKVSEVPLGMGEPLLSPIAHPEEAVACGGMEQLEPCVNLEGILPRETPLLEEPGEAFQLLDQVPLEDAMKLFDCSPIHSDIEAPLDEMGAGGQAALPGEAAMREGCFSGEDSSSDIRSLNLPDELLSFDYSVPEILSAVTSLDYLYDVNSFGEGPPWESRLGSPPPLPRLLRSPAGLEEKRRNLGPGNRARKPERASMQGGNRETLEGNATLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEL9</accession>
    <name>A0A6I9YEL9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 58</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LRRC58</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922445.1">
        <property type="nucleotide sequence ID" value="XM_014066970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEL9"/>
    <dbReference type="GeneID" id="106549346"/>
    <dbReference type="KEGG" id="tsr:106549346"/>
    <dbReference type="CTD" id="116064"/>
    <dbReference type="OrthoDB" id="1053178at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF53">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013922445.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="39189" checksum="73CA76128CCDA1AA" modified="2020-10-07" version="1">MAEEGGQAEAEARRAGEEGELRLRGAAEQSSRRLVVPLSWLSPAGELPAGLGSWYPALEVLDVSGTGLSVLGPELLDLAHLHTLLAKNNRLGCRGSLPKGLASAPLGRSLRVLNLSGNRFTHFPASLLGLSRLQSLSLGSNRLQSIPPAIQELSSLEFLYLGGNFITSIPPELANLSSLNYLVLCDNRIQSIPPQLAQLHSLRSLSLHNNLLTYLPREILNLVQLEELSLRGNPLVVRFVRELTYNPPSLQELAGRTIKTRNIPYVTSDLPGNLFRYLNLASNCPNPKCGGVYFDSCVRQIKFVDFCGKYRVPLMHYLCSPECSSPYSSASQSSNSQSESDSEDEAIVAARRMQKVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEX8</accession>
    <name>A0A6I9YEX8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Solute carrier family 35 member G3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106549556</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SLC35G solute transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922722.1">
        <property type="nucleotide sequence ID" value="XM_014067247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEX8"/>
    <dbReference type="GeneID" id="106549556"/>
    <dbReference type="KEGG" id="tsr:106549556"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF32">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="9">
        <location>
            <begin position="48"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="9">
        <location>
            <begin position="199"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="343"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922722.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="40435" checksum="56D1CC2970D94288" modified="2020-10-07" version="1">MASDPGPSAKATWTVSDLLRFSPPPCSPAPPPRRPAWHRYRLSESMKGLLVALLGGGVPAGFVAPFTRIVNEASGMPSLEILFFRCCLHLLFAGYLRFRKVPCFGPPEVRQRTLLHAAVNVISIGCAYSSFMMVPSGNAATVRKGSSTISSVLLAFCIENTRLTGYDWFGLVGSTLGLLIMVVPDLLSLDKSTQIYDTFGYVLACSGGVALALGLVIFRTLDFPAKLMTVAFLFGVVGSLVCCPMMSLLQDPVMTLDLYTWLYVVSICILALFSFLCASYAVTKAHPALVCAVLHSEVVVTLAVQYYVLREAVTPFDIMGAGVILGSIAIITAQNISCHPADEEEGPVAQQRGAKYAKEPVGRSGGHPADCSAGGDPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGN6</accession>
    <name>A0A6I9YGN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="12">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106550163</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013923458.1">
        <property type="nucleotide sequence ID" value="XM_014067983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGN6"/>
    <dbReference type="GeneID" id="106550163"/>
    <dbReference type="KEGG" id="tsr:106550163"/>
    <dbReference type="OrthoDB" id="9215500at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000149">
        <property type="entry name" value="Glycogen phosphorylase, muscle form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF29">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE, BRAIN FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923458.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="60418" checksum="71A045022D47BB3A" modified="2020-10-07" version="1">MHSPHRVIKSSLMFATNHSGSLSTTWHTMVEEADDWLRYGNPWEKARPEYMLPVHFYGRVDHTPNGVKWVDTQVILAMPYDTPVPGYKNNTVNTMRLWSAKAPNDFNLEEFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALSIPELMRIFVDVEQMEWDKAWEITKRTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYIINQKHLDYVASLFPGDVDRLRRMSVVEEGDCKRINMAHLCVIGSHAVNGVAQIHSEIVKNSVFKDFYDIEPEKFQNKTNGITPRRWLLLCNPGLSEVIVERIGEDFLTDLSQLKKLLDFVNDEAFIRDVAKVKQENKLKFSAYLEEQYKVKINASSMFDVHVKRIHEYKRQLLNCLHIITLYNRIRKDPKKSYVPRTVMIGGKAAPGYHMAKKIIRLITAIGEIINNDPYIGDRLKVIFLENYRVSLAEKGKPVLEAGWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGQ8</accession>
    <name>A0A6I9YGQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein Hox-A7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">HOXA7</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923075.1">
        <property type="nucleotide sequence ID" value="XM_014067600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGQ8"/>
    <dbReference type="GeneID" id="106549844"/>
    <dbReference type="KEGG" id="tsr:106549844"/>
    <dbReference type="CTD" id="3204"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000017">
        <property type="entry name" value="Homeobox protein antennapedia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF12">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="128"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="130"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="188"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923075.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="25548" checksum="57B1A9A5BF6AE5EA" modified="2020-10-07" version="1">MSSSYYVNALFSKYTTGAASLFQNAEPTSCSFATNSQRSGYGPGASAFTSSMPALYNVNSAIYQTPFSSGYSLGSDTYNLHCSSFDQNIPVLCNDLTKSSCEKAEESNLHSQAESNFRIYPWMRSSGPDRKRGRQTYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKEHKEDAVGGAAAANEAAPATTSVEKVEEEEEEEEEDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGR4</accession>
    <name>A0A6I9YGR4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IgGFc-binding protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923371.1">
        <property type="nucleotide sequence ID" value="XM_014067896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGR4"/>
    <dbReference type="GeneID" id="106550099"/>
    <dbReference type="KEGG" id="tsr:106550099"/>
    <dbReference type="OrthoDB" id="5945029at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035234">
        <property type="entry name" value="IgGFc-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534">
        <property type="entry name" value="IGGFC_BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534:SF2">
        <property type="entry name" value="VWFD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17517">
        <property type="entry name" value="IgGFc_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFD" evidence="2">
        <location>
            <begin position="914"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013923371.1"/>
        </source>
    </evidence>
    <sequence length="1052" mass="115893" checksum="04A6DF1FF20B7BC8" modified="2020-10-07" version="1">MQDYQKYPSDTKYHLQITGHHPSTVIKVSVSKYKFWKTLVISKGITTTVKLPSTFGMSGSHYFDMTVFIEANKDISVFAQGHRRSSAGTTLVLPVHQLDRVYHVVTPNDSNTDGVKEFAIVAQEAATRVDVSLKGDVLLNGQAYPAGSKLSVNLEAFQALQLQSSDDLSGTRIESKEPVAVFSGHSCIMKDNACNQVFEQLLPSSSWGTFFIVPLLPFQNRSGTLYITASQNTNVKYQHGDVLSSHTLESGEVMQLSKYSSEPVSISASARIQVLYFSTGGESQDQRPLLLNIPAVTSFCRSYDLPYVKQLNNYAIIVAKTSDPSGIRIQRRTFAKSQWKSVPDTEYHWTEYSLARRGGVLSSEGLLVPLGVFIIGFSQYDGFGSQGHCTSRTCNVSSRGKKFLTAFMYNYYHYQNKKLELYVSGYHPATNITVSTNKGKIHRTINEGELASIELSLYLEMMGSDIFNKAVLIEADKDISVSSYSGVAVSVGGTLIYPIDQLGQLYYVVTPTEDSSLNLKEFAAVAHEDFTSVTIHLQGTVTFKNKVYHAGSILMTDLKAFEAIQLQSTEDLSGTRIESTKSVAVLSGHSCVTKFTFCDHVVEQLLPVQSWGTTFIVPPLSFQTKFDIAYIVSAENTLLKYQSSSRKESRTLVAGEVVQLEIPASQPLFISADARIQVVFFFTGAKRGDGYYEPFLMNIPAITCYCNSYHIYGMKNFTNHAMIIAKSSESGRITMEGRTIRWTQIPGTKYSWAEEDVDITDRALSVEHENSPFGLFVFGGGKADGYGTAALCSSVPISCPENSHHEACGNGCPATCSDRSAPSTCNNTCAQICQCDEGFVRSGEKCVPVDTCNCIYQGVTYKAGEEFWGDEDCQSHCKCDAKLGKAVCRKSSCKGKRKCSVVNGVQGCHPVTYKTCIGTGDPHYTTFDGKKYDFMGTCIYQMAGLCSQDPSLTPFLVTVENNHRGNKAVSFTKVVTLEIYNFTISMSQEFPEKIQCLDILCSNLPEHQGEVHSGCGDFLNVSLKCWASGLHLFPQMLHYSSETGAEYQAATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGZ5</accession>
    <name>A0A6I9YGZ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger and SCAN domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106549999</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923249.1">
        <property type="nucleotide sequence ID" value="XM_014067774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGZ5"/>
    <dbReference type="GeneID" id="106549999"/>
    <dbReference type="KEGG" id="tsr:106549999"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000514">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000053">
        <property type="entry name" value="zinc finger protein 182 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000870">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001009">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000051">
        <property type="entry name" value="zinc finger protein 585A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000028">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="15">
        <location>
            <begin position="165"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="588"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="615"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="643"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="676"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="699"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="727"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="755"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="830"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="259"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="338"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923249.1"/>
        </source>
    </evidence>
    <sequence length="904" mass="102451" checksum="1ED798CB01E9EF13" modified="2020-10-07" version="1">MSTAQEAEVSQSLLHHNMDPEIKMEGRDSIDPKPGEGRTHFLNIGKSKVEPGRNAKAELEENPSKNWDAQLQEFLKTLEEPQGRSEVPQCLEPKLWSGPKLSGMSSQGGMDASKWLKEMCISQMEAHCLGTTQEDWDTLLDVGHGGKEKIGELAEGAIRAEARCRRFRTLGYKEAKGPQDFCQQLQELCREWLKPEKHTKEQILDLVILEQFLSALPLDIQNWLRRNGPETCVHAVSLAEEFLLKRAETQTWEEQVIEISEEEDIEKAPKSSQLPSEPPKDEDFGLVKPKRDGMKNHRGIGWLNEGGEPKLKNSEKRKPLRKFLKAAIKTDLLAFEETDGNGDTATKQNGSDVEKGVGPSVPPQSVGSGLSDPAVQEEEEMNDNGSEESMNQSLKVVRTESPDAEEKPFRCWHCGQIFRNSSHLVSHERTHVGEKLYKCSHCGESERTHTGQRPHKCPHCGCIFGYHGHKRIFVEERPHVCSDCGKSCSQKSDLLKHQRTHTESKKSYKCSACGKTFKNGSSLKAHQRTHTGEKPYTCLYCGKCFVWSSQCRLHERIHTTECSHCGKGFGQKSELVEHEKTHLVEDLLVCFACGKIFELSSELMAHMRTHKRKQFECSACGKTFMNSLQLTAHQKVHTGEKPHKCSHCGKSFSQRQSLIAHERIHTGEQPHMRLVCGKNVRNAPNLKSHERTHMGEKPYRCSHCDKSFRWSSHLLLHKRIHTGEKSYSCSDCGRTFDRRCNLLRHVKNHTGQRSHVCSDCGKSFISSSVLSRHQKVHVGEDVARCLECGKGFRQCMDQAEKVSKCPECIRRPSLGLDLRRAQMRLKEKTFQCSLCRKTFNNSSHLMVHQSVHTRVRPRQCLECGRDIIRRPNLIAPLKNLSGQNAEKCTECENKTVELDLGGKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI69</accession>
    <name>A0A6I9YI69_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein Hook homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HOOK1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the hook family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924058.1">
        <property type="nucleotide sequence ID" value="XM_014068583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI69"/>
    <dbReference type="GeneID" id="106550645"/>
    <dbReference type="KEGG" id="tsr:106550645"/>
    <dbReference type="CTD" id="51361"/>
    <dbReference type="OrthoDB" id="49395at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000024">
        <property type="entry name" value="Hook homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008636">
        <property type="entry name" value="Hook_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF36">
        <property type="entry name" value="PROTEIN HOOK HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05622">
        <property type="entry name" value="HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="8"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="169"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="250"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="485"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924058.1"/>
        </source>
    </evidence>
    <sequence length="724" mass="84583" checksum="73933754C816E92D" modified="2020-10-07" version="1">MEGKPADQLLCDSLILWLQTFNIAKSCREVQDLTNGVALAYVLHEIDPAWFDKAWLRRIKEDAGESWRIKASNLKKVLQGIMDYYHEFLTQQISEDLLPDLNQISEHSNPVELGRLLQLILGCAVNCEKKQEHIQVIMTLEESVQHVVMAAIQELMSKEILGFVPSDISGDVEQQLKRAVEELNEVIAEKEELKQRCQELDLQVAALQEEKCSLMSENEIMNDRLDQLDGSFDDTNTAVAKKYFHAQLQIEQLQEENFRLETTKDDYRVHCEELEKQLIELQHRNDELTSLAEESRALKDEIDVLRTVADKTNKLESTVEVYRKKLEDLNDFRRQVKILQETNMMYMHNTVSLEEELKKANAARSQLETYKRQSQEVHNKLSEESKRADALAFEMKQLEEKHETLIKEKERLIIQRDALKETNEELRYSQMQQDHMNQTGASTMKSHENLAAEILPVEFRDMFIRLQHENKMLHLQQEGSENEQIAGLQEKLEQKNRMINELETENRLSKERFGELQQQIEDLQKSLQEQESKSERERSSQLKQKIAAHMEKLNEVHDELQKKEALLADLQPDLNQNAQKIEELEAALHKKDEDMKSMEERYKMYLEKARIVIKTLDPKLKPASTEIMLLRKQLVEKDKRIETLEGQCKSSRIQEHEEKLIVSAWYNQSLAFHKLGMESRLLRVNTAYRDGQSYPPAQSFLAQQRRLTNSRRNLPVKAPPTSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIZ9</accession>
    <name>A0A6I9YIZ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dual specificity protein phosphatase 14-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106550521</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923929.1">
        <property type="nucleotide sequence ID" value="XM_014068454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIZ9"/>
    <dbReference type="GeneID" id="106550521"/>
    <dbReference type="KEGG" id="tsr:106550521"/>
    <dbReference type="OrthoDB" id="285418at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000049">
        <property type="entry name" value="Dual specificity protein phosphatase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020420">
        <property type="entry name" value="Atypical_DUSP_subfamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46495:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46495">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01910">
        <property type="entry name" value="ADSPHPHTASEB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="40"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="102"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923929.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="23741" checksum="6C362FDCDD533C4D" modified="2020-10-07" version="1">MYAEPSTMNFRNHGFFRRSPPTSVAKTSPAAGNTLSVLGGIAQISPCLYLCSGNAASNRHMVYSRAVTCIVNATMEIPNANWPDIDYVKVPVPDLPHAPLSLYFDSVADRIHQTGKKNGRTLVHCVAGVSRSASLCIAYLMKYHRLSLLDAHEWVKSRRPVVRPNAGFWRQLIEYECKLFGKNTVKMVPSPVGLVPDVYEKETRGLVPLWNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJS7</accession>
    <name>A0A6I9YJS7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">D(1) dopamine receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106550609</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924020.1">
        <property type="nucleotide sequence ID" value="XM_014068545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJS7"/>
    <dbReference type="GeneID" id="106550609"/>
    <dbReference type="KEGG" id="tsr:106550609"/>
    <dbReference type="OrthoDB" id="6021915at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004952">
        <property type="term" value="F:dopamine neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042311">
        <property type="term" value="P:vasodilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000691">
        <property type="entry name" value="Predicted vertebrate-like dopamine D1-like G-protein coupled receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001413">
        <property type="entry name" value="Dopamine_D1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF123">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00565">
        <property type="entry name" value="DOPAMINED1AR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="37"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013924020.1"/>
        </source>
    </evidence>
    <sequence length="461" mass="51847" checksum="B49D79D0095BFBAC" modified="2020-10-07" version="1">MTNDPFMKKSQVEKTKISLRVLMAALLVLLILFTLLGNTLVCMAVIKFRHLRSKVTNFFVISLAVSDLFVAVLVMPWKAVVEVTGFWPFGGFCDIWVAFDIMCSTASILNLCIISMDRYWAISSPFCYERKMTQRVASVMIGVAWMLSVLISFIPVHLRWHKARELLPGEGSGSNLTWPLEANCDSSLNRTYAISSSFISFYIPVAIMIVTYTRIFRIAQRQIRRISSLERAVEHAQSCQSCECSHEVSLKNSFRKETKVLKTLSIIMGVFVFCWLPFFVLNCIVPFCNPDLQKIGKLPCISDTVFNIFVWFGWANSSLNPIIYAFNADFRKAFSAILGCGRLCPSNAVETVNFSNELVSYHHDTTCQKDLGTLSYPHFLPHAITLPGEEEEDLVSDKAPQSSRGNSAAVLSAIIHVEYETDLSLEKITPFTTHVLDEGRTKDDHAEGDGSSEHEPFPWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMA5</accession>
    <name>A0A6I9YMA5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Helicase-like transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HLTF</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925523.1">
        <property type="nucleotide sequence ID" value="XM_014070048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMA5"/>
    <dbReference type="GeneID" id="106551885"/>
    <dbReference type="KEGG" id="tsr:106551885"/>
    <dbReference type="CTD" id="6596"/>
    <dbReference type="OrthoDB" id="276744at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18071">
        <property type="entry name" value="DEXHc_HLTF1_SMARC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16509">
        <property type="entry name" value="RING-HC_HLTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF17">
        <property type="entry name" value="HELICASE-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="276"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="691"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="765"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="327"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925523.1"/>
        </source>
    </evidence>
    <sequence length="934" mass="105835" checksum="FEB4FF2158BA5C0D" modified="2020-10-07" version="1">MAWRFLRDFSWELDPALYGDKHYNVFTREFYPSVEDIMGIVTSGIDEDIDSAVLFGTLQGNVVGLRYYTGVVNNNEMVALQREPNNPYDKNAVKVNNVNGDQVGHIKKELAACLAYIMDNKLAVVEGVVPRGANNTFTMPVQLSFWGKEENKQVVLERLKMHGLKLAPPGKFSEFGFETGMTSGRGKTRYNAPSHGAIQMTTEQLKTEFDKLFEDLKEEDKTCEMEAAKAVMTPLLPHQKQALAWMILRENNNDLPPFWEERNDFFYNTIIHFAEKNRPENVLGGILADDMGLGKTLTMIAVILTNFHNGKPLPLEKKIDQMEEDCGLQQNASNSKEESDSFPSQSSRSYPKRVTGKKSKGTISCDTEPEQEVAEQIKTKGKKPKSRARKRKLDDVPVQPKKNEVDNVRGTLIICPLSVLSNWIDQLEQHVDPNIQLNTYVYYGSERNKDLIVLSKQDIVLTTYNVLATDYGGRDNSPLHKLKWLRVVLDEGHTIRNPNAQQTKAVLDLNAQRRWILTGTPIQNSLKDLWSLLSFLKLKPFTDRLWWHRTIQRPVTMGDERGLKRLQSLIKSITLRRTKTSKVNGKPVLKLPERKVFIQHITLTDEESAVYHSVKNETKAALSRYFTEGSVLAHYADVLGALLRLRQLCCHPSLCSSVSQSSSLESTSEEFRDMLIQKMKLVLSSGSDEECAICLDSLVDPIITHCAHVFCKTCICEVIQREKDYAKCPLCRKEVSAEHLIECHLEETHDEKNKQQWPSSSKVNALMHALIELRKQNPAIKSLVVSQFTKFLSVIETSLKESGFVFVRFDGSMNQKQRVEAIQQFQNNETGSPTIMLLSLKAGGVGLNLTAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQDVIITKFIIKNSVEENMLKIQNKKRELAAGAFGTKKPSTGETKMNEIRTLMDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMA7</accession>
    <name>A0A6I9YMA7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Fumarylacetoacetase</fullName>
            <ecNumber evidence="4 14">3.7.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Fumarylacetoacetate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106551370</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>4-fumarylacetoacetate + H2O = acetoacetate + fumarate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10244"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18034"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="3.7.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.2" evidence="4 14"/>
    <dbReference type="RefSeq" id="XP_013924945.1">
        <property type="nucleotide sequence ID" value="XM_014069470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMA7"/>
    <dbReference type="GeneID" id="106551370"/>
    <dbReference type="KEGG" id="tsr:106551370"/>
    <dbReference type="OrthoDB" id="9971669at2759"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00341"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004334">
        <property type="term" value="F:fumarylacetoacetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902000">
        <property type="term" value="P:homogentisate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.230">
        <property type="entry name" value="Fumarylacetoacetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005959">
        <property type="entry name" value="Fumarylacetoacetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015377">
        <property type="entry name" value="Fumarylacetoacetase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036462">
        <property type="entry name" value="Fumarylacetoacetase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01266">
        <property type="entry name" value="fum_ac_acetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43069">
        <property type="entry name" value="FUMARYLACETOACETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43069:SF2">
        <property type="entry name" value="FUMARYLACETOACETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09298">
        <property type="entry name" value="FAA_hydrolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63433">
        <property type="entry name" value="Fumarylacetoacetate hydrolase, FAH, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 14" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarylacetoacetase N-terminal" evidence="18">
        <location>
            <begin position="95"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="17">
        <location>
            <begin position="161"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605959-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605959-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF09298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013924945.1"/>
        </source>
    </evidence>
    <sequence length="388" mass="43261" checksum="AE34224F9B868667" modified="2020-10-07" version="1">MGNTTSCCDSSSPKLPSNDHSQLESFQPDTELSCNLQHISDRENIDGKRSWVSCKVYQLCGFGVILVSNILASGFVTVFHCQLRSLLSGALLYDDERPRHRIGVAIGDQVLDLSSVKHLFDGPVLSKQHHVFEQVLRVFFWFFLRRTVLQKVIFSKSPLVAASKRLDIELEMAFFVGPGNMLGVPIPIGEAHKHIFGMVLMNDWSARDLQKWEYVPLGPFLGKSFGTTISPWVVTMEALMPFALPNPTQDPKPLPYLFDDQPYTFDINLFVAIKGDGMSKPANICRSNFKHMYWTMKQQLTHHTVNGCNLKPGDLLASGTISGPEPENFGSMLELSWNGTKSIDLGQGQSRTFLQDGDEVIMTGYCQGKGHRIGFGECSGKILPARMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNQ8</accession>
    <name>A0A6I9YNQ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transcriptional regulator Kaiso</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZBTB33</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925786.1">
        <property type="nucleotide sequence ID" value="XM_014070311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNQ8"/>
    <dbReference type="GeneID" id="106552125"/>
    <dbReference type="KEGG" id="tsr:106552125"/>
    <dbReference type="CTD" id="10009"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18219">
        <property type="entry name" value="BTB_POZ_ZBTB33_KAISO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000749">
        <property type="entry name" value="Transcriptional regulator Kaiso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000077">
        <property type="entry name" value="transcriptional regulator Kaiso isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000235">
        <property type="entry name" value="Zinc finger and BTB domain containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF27">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR KAISO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="13">
        <location>
            <begin position="47"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="570"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="230"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="305"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925786.1"/>
        </source>
    </evidence>
    <sequence length="692" mass="76241" checksum="3FF3EB2FA8BFDCEE" modified="2020-10-07" version="1">MEPGAMSEAGGKTPGMETRKLITATDNQYSGMLLKALDEQRGLGLFCDVTVIVEDRKFRAHRNILSASSTYFHQLFAVAGQVVELNFIRGEVFAEILNYIYSSKIVRVRSDLLDELIKSGELLGVKFLAELGVPLQQVKSMSSEATPSASELPIPEGNGPKAQKLPAPSEPQPVKLGKPIITESFSLYRESCDSAKITISDSEDDDDDVIFCSELVPSKERSVETKVITQIPPCPNGSSEVASSSNSHSLPQPVTTAPPRLLDSSTVLPNPAENQKLLDPLPPPAPKTTTPNVLVLNSARVDVAPGVGPSHEIEVLPIPEENQQPSTNDSLTDMETNVIDEEEEEDEDVEDDDDILGLSSPGSASSSSLVQQPTTPKPTVAADSTAAQKKQVVNFLPEPVSQPNEFKIQISDVLSGGLKDAPPNVAPKHVTEGQKIITLDKTTEIESLSTGCKVYANIGEDTYDIVIPVKNEADGEVQLDNVPHNSGNDSANRKRLKVKHDDHYELIMDGKVYYICIVCRRSYVCLTSLRRHFNVHSWEKKYPCRYCEKVFPLAEYRTKHEIHHTGERRYQCLACGQCFINYQVMATHLRSVHSQDPTGESKLYRLHPCRSLQIRNYAYISGSSNNIPVANDNALVYAGSPPKETPQEPVPNQPVKPMTWDDIFVQQGNETLFKQNPSDGTSEFEFVIPESY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNY5</accession>
    <name>A0A6I9YNY5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106552179</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925866.1">
        <property type="nucleotide sequence ID" value="XM_014070391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNY5"/>
    <dbReference type="GeneID" id="106552179"/>
    <dbReference type="KEGG" id="tsr:106552179"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="452"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="295"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925866.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="56594" checksum="61BBADC4E4F4ABF4" modified="2020-10-07" version="1">MAPKNEIQYPAIVQLLFHFKWTLVGVFASDTETGEHFMRTFTPLLIRNGICVVISQRFSMSRPTEPFRDAISKWRQVNVFVFFTDYSTMLDGISQIHFILEGLSGPIEGKVWIITLFGKLPMKYHKSYKYVHSIWNFYIQKRAYDKTFQEYIFVEPQFEDQSFNCSFSRHVFSVKGQRRCTQKLHPFLQKNEFCNLSEMKMYLDKNGDLVADVNIENLSLPQSKCAENCHPGFVKQAQEGEPVCCYKCIACPEGTISTQKDTEKCTKCPDDQYPNEDKVQCIPKRISFLSFEEHLGIILVSFALLLFLTTGLVLITFIKYLETPIVKANNRDLSYILLVSLLLCFLSSFLFIGQPRKVTCLLRQTVFSIVFSVAVSSVLAKTITVVLAFLATKPGNRLRKWLGKSLANSIILSCSGIQIFICAMWLGIFSPFPNSDLHSQPGEIILQCNEGSVTMFYVVLGYMSFLAAICFTVAFLARNLPGAFNEAKLITFSMLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ53</accession>
    <name>A0A6I9YQ53_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dynein light intermediate chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106552803</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 14">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
            <location evidence="1 14">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the dynein light intermediate chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926643.1">
        <property type="nucleotide sequence ID" value="XM_014071168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ53"/>
    <dbReference type="GeneID" id="106552803"/>
    <dbReference type="KEGG" id="tsr:106552803"/>
    <dbReference type="OrthoDB" id="27603at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008467">
        <property type="entry name" value="Dynein1_light_intermed_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022780">
        <property type="entry name" value="Dynein_light_int_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688:SF1">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 LIGHT INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05783">
        <property type="entry name" value="DLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013926643.1"/>
        </source>
    </evidence>
    <sequence length="264" mass="29433" checksum="DE59D5A182638116" modified="2020-10-07" version="1">MRSLFVAAAVGKMAPVLAEKKLLEPDGPLAADLPSEEEEGQSLWLSILSEVSTRSRSKLPSGKNILVFGDDGSGKTTLLTKIQGVEHGKKGRGLEYLYLNVHDEDRDDQTRCNVWILDGDLYHKGLLKFAVTAESLQETLVIFVADMSRPWTILESLQKWASVLREHIDKLKIPPEEMRNMEQQFVKIFQEYVEPEGGYQGSPQRRGPSGQEEENIALPLGENVLTHNLGIPMVVVCTKVSLKSPAPPPIYTMELNLVTLKNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRL2</accession>
    <name>A0A6I9YRL2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inactive hydroxysteroid dehydrogenase-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HSDL1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926270.1">
        <property type="nucleotide sequence ID" value="XM_014070795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRL2"/>
    <dbReference type="GeneID" id="106552501"/>
    <dbReference type="KEGG" id="tsr:106552501"/>
    <dbReference type="CTD" id="83693"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000137">
        <property type="entry name" value="Hydroxysteroid (17-beta) dehydrogenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052149">
        <property type="entry name" value="17-beta-HSD3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44889">
        <property type="entry name" value="INACTIVE HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44889:SF1">
        <property type="entry name" value="INACTIVE HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013926270.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36457" checksum="4E11015DE397ACAB" modified="2020-10-07" version="1">MAAVDSFSLLMREIGRSYHYCMEMLAVVGAFYVSKVCVTVLSDTYVLIRVHFVPRIFGRPDLSKRYGKWAVVTGGTSGIGLSYAKELASCGIDLILISQNQEELEASAKEIMETYNISTMTVVTDFNRGREAYPVIKKVLEDKEIGVLVNNVGVLNPLPNSFTNLTEAQIWEFINVNIGAANMMAYLVLPGMVQRKKGAIVNISSISCCNPCPRYTAYSASKTYLDHFSRSLHYEYAPQGIFIQSLIPFFVCPNVTKLSSTLSKMSFFVPSPDEYACHAITTLGVSRRTTGYWPHTLLMIFARFVPEWFWAWMVPRINYCQKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSM9</accession>
    <name>A0A6I9YSM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Epithelial discoidin domain-containing receptor 1</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">CD167 antigen-like family member A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Discoidin receptor tyrosine kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Tyrosine kinase DDR</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">DDR1</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 32">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homodimer. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013927558.1">
        <property type="nucleotide sequence ID" value="XM_014072083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSM9"/>
    <dbReference type="GeneID" id="106553557"/>
    <dbReference type="KEGG" id="tsr:106553557"/>
    <dbReference type="CTD" id="780"/>
    <dbReference type="OrthoDB" id="6071166at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038062">
        <property type="term" value="F:protein tyrosine kinase collagen receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000007">
        <property type="entry name" value="Discoidin domain receptor tyrosine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000589">
        <property type="entry name" value="Discoidin domain receptor tyrosine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000053">
        <property type="entry name" value="Epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1190:FF:000002">
        <property type="entry name" value="epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048525">
        <property type="entry name" value="DDR1-2_DS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF333">
        <property type="entry name" value="EPITHELIAL DISCOIDIN DOMAIN-CONTAINING RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21114">
        <property type="entry name" value="DDR1-2_DS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0421">Lactation</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0635">Pregnancy</keyword>
    <keyword evidence="17 31" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 27" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027074500" description="Epithelial discoidin domain-containing receptor 1" evidence="27">
        <location>
            <begin position="18"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="29">
        <location>
            <begin position="28"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="476"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00063678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00074969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00078085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00079442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00082942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013927558.1"/>
        </source>
    </evidence>
    <sequence length="773" mass="87202" checksum="F29B91A118ECAAF7" modified="2020-10-07" version="1">MGPRMLWFLLLASLGTSELEEHFDPAKCRYALGMQDRTIPDEALSASSSWHDSTAAKHSRLGLSEGDGAWCPAGPVYPNDAEYLEIDLRRLHFLTLVGTQGRHAGGHGKEFARSYRLHYKRDGHHWMPWRDRWNNEVISGNENTDDVVLKDLGPPIIARYVRFYPRSDRVMSVCLRVELYGCVWQDGLQSYTAPLGQVMPLVPEPVYLNDSTYDGSSVSKLHFGGLGQLTDGVVGLDDFTQTKEFRVWPGYDYVGWSKRSSPEGFIKMEFEFDRLRSFTYMKVHCSNMHTRDVRIFEQVDCLFKHSLATAWEPQPVSLQLSVDVRDPSARSVTIPLEGRIGLFVQCHFYFAAEWMLFSEIAFLSSELGLGPRSLLLSNPAYHLLLATYAQPMGRPAFPPPSQVKPINTNACGIDYMEPELAASQPSFQNNVPHYAEADIINLQGVTGGNTYAVPAVAVDALAGKDMAVGEFPRERLIFKEKLGEGQFGEVHLCEVENLQDLPSLEFPFNVRKGRPLLVAVKILRSDATKNARNDFLKEVKIMSRLKDPNIVRLLGVCVRDDPLCMITEYMENGDLNQFLSGHELEDKLGGSPNGRPTISYPTLVHLGAQIASGMAFLASLNFVHRDLATRNCLVGEGFTIKIADFGMSRNLYAGDYYRIQGRAVLPIRWMAWECILMGKFTMASDVWAFGVTLWEILMLCQEQPYSQLTDEAVIENAGEFFRHQGKQVYLCRPPTCPLAVYEMMLRCWAREAKDRPSFQHLHCFLAEDALNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSV0</accession>
    <name>A0A6I9YSV0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="7">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PGD</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013927019.1">
        <property type="nucleotide sequence ID" value="XM_014071544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSV0"/>
    <dbReference type="GeneID" id="106553110"/>
    <dbReference type="KEGG" id="tsr:106553110"/>
    <dbReference type="CTD" id="5226"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001852">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="8">
        <location>
            <begin position="109"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927019.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="33207" checksum="D9370BB9039AE91D" modified="2020-10-07" version="1">MEQGRRKTQRRQYCWDEVPLLSRGDIIIDGGNSEYRDTTRRCKELKEKGILFVGSGVSGGEEGARYGPSLMPGGAKEAWPHIKTIFQSIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLAMDHDEMSKVFQEWNKTELDSFLIEITADILKFRDADGKHLLPKIRDSAGQKGTGKWTAISALEYGVPVTLIGEAVFARCLSSLKDERVQASKLLSGPKAQAFSGNKAAFLEDIRKALYASKIISYAQGFMLLRQAAKEFGWTLNYGAIALMWRGGCIIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUC1</accession>
    <name>A0A6I9YUC1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Myelin expression factor 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106554102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928178.1">
        <property type="nucleotide sequence ID" value="XM_014072703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUC1"/>
    <dbReference type="GeneID" id="106554102"/>
    <dbReference type="KEGG" id="tsr:106554102"/>
    <dbReference type="OrthoDB" id="610462at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF15">
        <property type="entry name" value="MYELIN EXPRESSION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="73"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928178.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="20517" checksum="32595CF118618C9F" modified="2020-10-07" version="1">MNKYDLNGRPLNIKEDLDGEHARRALQRGSGPFTGGHGPDIAPGLMNLPPSILNNPNIPPEIISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAICILTSQPDQRAMLSSFICVSKLKNMVPTIFDFREGLVVCKLPEIYLFDVFMLLNCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUG8</accession>
    <name>A0A6I9YUG8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Magnesium-dependent phosphatase 1</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MDP1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Magnesium-dependent phosphatase which may act as a tyrosine phosphatase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013927681.1">
        <property type="nucleotide sequence ID" value="XM_014072206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUG8"/>
    <dbReference type="GeneID" id="106553661"/>
    <dbReference type="KEGG" id="tsr:106553661"/>
    <dbReference type="CTD" id="145553"/>
    <dbReference type="OrthoDB" id="2865258at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07501">
        <property type="entry name" value="HAD_MDP-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000127">
        <property type="entry name" value="Magnesium-dependent phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035679">
        <property type="entry name" value="MDP-1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01685">
        <property type="entry name" value="MDP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01131">
        <property type="entry name" value="C1.5.2:_MDP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927681.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="16181" checksum="9D3C1A131FE25D7B" modified="2020-10-07" version="1">MCRKPALVVFDLDYTLWPFWVDTHVDPPFHKKSDGSVRDRNQRPVQLYPEVPEVLHQLDSEGIAMAAASRTGEIQGARQLLDLFGLNRYFRYTEIYPGSKITHFQRLHQQSGIPFHKMLFFDDESRNIHDVGTLGILKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUV8</accession>
    <name>A0A6I9YUV8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tripartite motif-containing protein 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106553755</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Neurotoxin that produces dose-dependent hypolocomotion and hyperalgesia in mice. May directly act on the central nervous system, as it is 6500-fold more potent when administered intracerebroventricularly than intraperitoneal.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ohanin/vespryn family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927796.1">
        <property type="nucleotide sequence ID" value="XM_014072321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUV8"/>
    <dbReference type="GeneID" id="106553755"/>
    <dbReference type="KEGG" id="tsr:106553755"/>
    <dbReference type="OrthoDB" id="9410880at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0528">Neurotoxin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0800">Toxin</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="292"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="164"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927796.1"/>
        </source>
    </evidence>
    <sequence length="479" mass="54817" checksum="428C71A18FB95A30" modified="2020-10-07" version="1">MAAAARSPLRDLYEEVTCPICLECFKEPVTITICGHNFCRACLSCYGQGPEISGTSCPHCRKTFDHRQNIIPNRLLANVVEIAEKFRRAEESPAEANLCEKYHRPVKVICGQQTVLHCAICGPTKPTKSNHGSPTMQKTNDTTTEHTDRNTLVEMLKEGKQNVLAQQSDKKKKSKDLLRQIKAERAEATSLFKDLHHFLEEQEKQFLAQMVEVEEEITRKRDSFMSFFSQELSLYETIIQKIQTSQQPNNALFKEILKSFQSEEKITDRPSFQPALKWRIWDFCDINHTLKEITKRFKDTWLSGLQLQKANITLDPDTAHPQLTLFLDGKTVELGERCQELPNNPARFDFLSVVLGREEFKGGRHFWEVNVESQKEWAVGISLKSVKRKDSFSLNTAEGISMLGRWRGDYRFSLPPNPLPHIPDKKFKKIRVCLNCAVGKISFFDAETGILLYSTTSFPGEVVLPFFWLGSYAQLSLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV92</accession>
    <name>A0A6I9YV92_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">P2Y purinoceptor 13-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106553553</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927555.1">
        <property type="nucleotide sequence ID" value="XM_014072080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV92"/>
    <dbReference type="GeneID" id="106553553"/>
    <dbReference type="KEGG" id="tsr:106553553"/>
    <dbReference type="OrthoDB" id="6163051at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045028">
        <property type="term" value="F:G protein-coupled purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000049">
        <property type="entry name" value="G-protein coupled receptor 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008109">
        <property type="entry name" value="P2Y13_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233:SF10">
        <property type="entry name" value="P2Y PURINOCEPTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
        <property type="entry name" value="P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01735">
        <property type="entry name" value="P2Y13PRNCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="42"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927555.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="38295" checksum="87B71E87D9855D76" modified="2020-10-07" version="1">MNGSINASNWTSASGQCPRDTRTSHVVFPILYSILFLLGFLLNSVAVVAFFQIPTTSSFIVYLKNTVVSDSIMTLMLPFKILTDSEMGFWQLKAFVCRFSAVIFYQTMYISIVLLGLISLDRFLKIIQPFGKFWLHKITSAKVVSWSVWLFFFVLSLPNVILTDREATSLPVRKCASLKSDLGLKWHEAVNYICQIVFYTVLILMLLFYVIIAKKIYSSYKRTKIAECKIKQRAKSKVFIIVAVFFICFAPYHFVRVPYTLSQSGQSMSCTKQNQLFIAKESTLWLATANICMDPLIYVFLCRKFIEKAFCSKLQKSRYTIQESTTLALDTGIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWK6</accession>
    <name>A0A6I9YWK6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dual oxidase 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929033.1">
        <property type="nucleotide sequence ID" value="XM_014073558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWK6"/>
    <dbReference type="GeneID" id="106554824"/>
    <dbReference type="KEGG" id="tsr:106554824"/>
    <dbReference type="OrthoDB" id="6019201at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000004">
        <property type="entry name" value="Dual oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF144">
        <property type="entry name" value="NAD(P)H OXIDASE (H2O2-FORMING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013929033.1"/>
        </source>
    </evidence>
    <sequence length="608" mass="69428" checksum="9B793762A6767775" modified="2020-10-07" version="1">MSWAAGTGRSPNNPREMINGVTGWLDGSAIYGSSHVWSDSLRSFSQGKLLSGKKEGFPKQTEANSLMWKALDPATGKGGTEGIFDFGNAKANENVFLQAMSVVWFRYHNHWAEQLAKQNTNWSDEDLFQHARKRVIATYQNIVFYQWLPSVLGNTSIKNYEGYKQHVDSRISTEFLVASALFLGTKVPSGVYMRNSVCFFQNVTDVNGEPSPALRLCNNYWSRQNPNLQTSEDVDNLLLGMSSQITKKEDNFVVEDLRDYWYGTVRYSRMDFVASWIQRGRDIGVSTYNKVREFFNLQPAKNWTDINQKNKTILESTADLYGDSVESLELLLGGMLEEDEALFRAILEDQFVRLRDGDRFWFENRKNGLFTAEEIAAIKNTTLHDVLAAVMYTKNIQADVFMWKNGDPCPQPRQMDLQGLPSCKQMTVINYFKGSAAGFGILIAALCCLPLVSLFIAWIVAFFRRRSFRKLQKKQNPTSHPDVSAEGTQALEWHGFKIDSSLVYIQLKENKVIKVLDGKLAVVRSITLRSEQKVETIISNNRGNRTILLKIPKEYDLVLQFKMEAECRDFLGHLKTYLEGNELTLQVSMMREQQLLKKAVTKEQRKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWM3</accession>
    <name>A0A6I9YWM3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cdc42-interacting protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106554269</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928386.1">
        <property type="nucleotide sequence ID" value="XM_014072911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWM3"/>
    <dbReference type="GeneID" id="106554269"/>
    <dbReference type="KEGG" id="tsr:106554269"/>
    <dbReference type="OrthoDB" id="8783038at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057870">
        <property type="entry name" value="HR1_TOCA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25610">
        <property type="entry name" value="HR1_TOCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="REM-1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928386.1"/>
        </source>
    </evidence>
    <sequence length="81" mass="9325" checksum="D5EC03DFC9325A34" modified="2020-10-07" version="1">MKDVYQKTPQMGDPNSLEPKISETLGNIERLRLEIQKCEAWLADAESRMLSNRPDAVTRYNRHVDPASALNNNNCTHEKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX63</accession>
    <name>A0A6I9YX63_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Arginine-glutamic acid dipeptide repeats protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">RERE</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928546.1">
        <property type="nucleotide sequence ID" value="XM_014073071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX63"/>
    <dbReference type="GeneID" id="106554416"/>
    <dbReference type="KEGG" id="tsr:106554416"/>
    <dbReference type="CTD" id="473"/>
    <dbReference type="OrthoDB" id="6147534at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11661">
        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000052">
        <property type="entry name" value="Arginine-glutamic acid dipeptide (RE) repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000003">
        <property type="entry name" value="Arginine-glutamic acid dipeptide (RE) repeats a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002951">
        <property type="entry name" value="Atrophin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859:SF12">
        <property type="entry name" value="ARGININE-GLUTAMIC ACID DIPEPTIDE REPEATS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859">
        <property type="entry name" value="ATROPHIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03154">
        <property type="entry name" value="Atrophin-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ELM2" evidence="17">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="18">
        <location>
            <begin position="129"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="202"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="280"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="585"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="692"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="464"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="535"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="587"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="692"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013928546.1"/>
        </source>
    </evidence>
    <sequence length="1084" mass="120533" checksum="7C1C16BFA9270BC9" modified="2020-10-07" version="1">MDSFFYILGYNPETRRLNSTQGEIRVGPSHQAKLPELQPFPSGDGDAVTRHEELVWMPGVNDCDLLMYLRAARSMAAFAGMCDGGSTEDGCVAASRDDTTLNALNTLHESNYDAGKALQRLVRKPVPKLIEKCWTEDEVKRFIKGLRQYGKNFFRIRKELLPNKETGELITFYYYWKKTPEAASSRAHRRHRRQAVFRRIKTRTASTPVNTPSRPPSSEFLDLSSASEDDFDSEDSEQELKGYACRHCFTTTSKDWHHGGRENILLCTDCRIHFKKYGELPPIEKPVDPPPFMFKPVKEEDEGLSGKHSMRTRRSRGSMSTLRSGRKKQPTSPDGRASPINEDIRSSGRNSPSAASTSSNDSKADSVKKSTKKIKEEASSPLKSSKRQREKAASDSEEPDRINAKKTKTQEISRPNSPSEGEGESSDSRSVNDEGSSDPKDIDQDNRSTSPSISSPQDNESDSDSSAQQQPSLQAANGPAQPPGPLPAQLPSLPAGSSIPSSAVVPHMSPSGAQPSSQPLAPSLPHSHIQQGPSLHPPRLPSPPHPPLQPLAGASGQPQGLPPPHPQPSLHGQIQALPHSLQAQPLLPHPAPLQPFALPPQALPASSSQSQGPLPPPAHPALQNAQDPAAFFLRQLFLSFSSCPSVRPCRFYKHLDRGYNSCARTDLYFMPLAGSKLAKKREEAIERAKREAEQKAREEREREKEKEKEREREREREREAERVAKASSSSHDSRLGESQLSGPAHMRPSFEPPPTTIAAVPPYIGPDTPALRTLSEYARPHVMSPTNRNHPFFVPLNPGDPLLAYHMPGLYNVDPTLRERELRERELREREIRERELRERMKPGFEVKPPELDALHPATNPMEHFARHGALTLPPTAGPHPFASFHPGLNPLERERLALAGPQLRPEMSYPDRLAAERIHAERMASLTNDPLARLQMFNVTPHHHQHSHIHSHLHLHQQDPLHQGSAGPVHPLVDPLAAGPHLARFPYPPGTIPNPLLGQPSHEHEMLRHPVFGAHYPRDLPGAIPPPMSAAHQLQAMHAQSAELQRLAMEQQWLHGHPHIHGGHLPSQEDYYSRLKKEGDKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXF4</accession>
    <name>A0A6I9YXF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Bromodomain adjacent to zinc finger domain protein 1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24 25 26" type="primary">BAZ1A</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25 26" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24 25 26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928290.1">
        <property type="nucleotide sequence ID" value="XM_014072815.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928291.1">
        <property type="nucleotide sequence ID" value="XM_014072816.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928292.1">
        <property type="nucleotide sequence ID" value="XM_014072817.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554201"/>
    <dbReference type="KEGG" id="tsr:106554201"/>
    <dbReference type="CTD" id="11177"/>
    <dbReference type="OrthoDB" id="332390at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045740">
        <property type="term" value="P:positive regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031445">
        <property type="term" value="P:regulation of heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05504">
        <property type="entry name" value="Bromo_Acf1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15627">
        <property type="entry name" value="PHD_BAZ1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000029">
        <property type="entry name" value="Bromodomain adjacent to zinc finger domain protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000300">
        <property type="entry name" value="Bromodomain adjacent to zinc finger domain protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037325">
        <property type="entry name" value="Acf1_Bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047171">
        <property type="entry name" value="BAZ1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46510">
        <property type="entry name" value="BROMODOMAIN ADJACENT TO ZINC FINGER DOMAIN PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46510:SF1">
        <property type="entry name" value="BROMODOMAIN ADJACENT TO ZINC FINGER DOMAIN PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WAC" evidence="22">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="DDT" evidence="21">
        <location>
            <begin position="418"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="1145"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="19">
        <location>
            <begin position="1441"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="269"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="648"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1199"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1351"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="648"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="666"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="698"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1210"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1277"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1316"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1358"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1374"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1409"/>
            <end position="1419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013928290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013928291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013928292.1"/>
        </source>
    </evidence>
    <sequence length="1549" mass="177817" checksum="44365E2EA717D744" modified="2020-10-07" version="1">MPLLHRKPFVREKPPADLRPDEHVFHCRVTNEIFRDYDEFFERTILCNSLVWSCAITGKSGLTYQEALESERKARQNIQNFPEPLIVPVLYLVTLTQRSRLHEVCDDIFAYIKNRYFVGELVEVLRNNGERLHCKILEIKPPVHQNGMANGHAKGVDGVTIIISDSDDSDLDTSSAQNGNEKAITDPFLYKYKVKPLKKQLYESVTVKASQICRRRHIFSYDRLKLFLKQHCESHDGIIKIKAISIAKYKIAEQTFFCLFPDNPPTFMFSSSSKQKRSSKQTSDDHEDKDAEEHFSPRNLSNIAKERVKLQKEKEHMRTSEKLKREKANAMKFKRKEKQDKEKRKEELKKLVEEERLKKKEEKERLKIEKEKEREKLREEKKKYLEHLKQWSKRREDMECDDLKELPVPTPVKTRLPPEIFGDAVMVLEFLHAFGELFDLQDEFPDGVTLEVLEEALVGNDSEGPLCELLFFFLTAIFQAMAEEEEDIAKDQIADAETKDLTEALDEDADPTKSALSAVATLAAAWPQLHQGCNLKNLDLDSCTLSEILRLHILASGADVTSANAKYRYQKRGGFDTTDDACMELRLSNPGLLKKLSCTSVYDLSSAEKMRILHALCGKLLTLVSTRDFIEDSVDVLRQTKQEFRELKAEQHRRERGAAAAKIRRKKEERLKGQEQKMKEKHEKLKEIEKNPAVELSISDEDREELDTSTESKGIETKEQEVDTMTDEEGNSSPNKKKGQGEQNGFKDFTRPVRTTDDKCELLTAEEEAALKQEQQNKEKELIEKIQKATVCTNISSLGRDRLYRRYWLFSSVPGLFVEEDYSGLTEDMLLPRPSSFHNNVQAHLSEKSQIVIKTGESLTAESTSKTCQDLETSAIVNVSQPIQKPNRWCFYSSQEQFDQLLEALNSRGYRESALKDVLLQEKSRICEQFSSFPIEKFYISEKTQNEAKPQFGRGKMQNGCDIVQKSAEKQLELRLRDFLLDIEDRIFQGTLGCIKVMDRYSWRAALENGQYELLNEENKENGVIKSVNENVEEMEIDNQIKILAKDRLVGLKPESRSTTSTNTSTPQPMNNTVHYLALALLQIQQGIERKFLKAPLDGSDGGRLHKTVLDRWRESLLSSASLSQIFLHLSTLERSVIWSKSILNTRCKMCRKKGDAESMVLCDGCDRGYHIYCIRPKLKAVPDGDWFCPECRPKQRSRRLSSRQRPSVESDEEAIEEIKEEDETSYEETGQSEDDYEEEEEDENIDNSDSQEDEKSLSKLGNPQVKLTLRTRASKLASNQLSTGRYNSRSQQNTPKQIGSSYKAPGKNIRKTKSAPPSGTKTLRLSSRITRHNQEMLKADAFIELTSHRRQRRGIKTTDTTPDSSSGSFGFRIIEEMNLRKEKSSPISVEKKPQDTDPPKRGRKRQSTESSQVPLNRRTSGRQGGVHELSAFEQLVVELVRHDDSWPFMKLVSKIQVPDYYDIIKKPIALNIIREKVNKCEYKVASEFIEDVELMFSNCFEYNARNTSEAKAGTRLQAFFHVQAQKLGLSVTSGYMNHVVPAAKKSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXN1</accession>
    <name>A0A6I9YXN1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thymus-specific serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PRSS16</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928370.1">
        <property type="nucleotide sequence ID" value="XM_014072895.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554260"/>
    <dbReference type="KEGG" id="tsr:106554260"/>
    <dbReference type="CTD" id="10279"/>
    <dbReference type="OrthoDB" id="1735038at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF11">
        <property type="entry name" value="THYMUS-SPECIFIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928370.1"/>
        </source>
    </evidence>
    <sequence length="404" mass="45555" checksum="4947E33A82ADCF26" modified="2020-10-07" version="1">MVKFGADTLISTDAINQRPGQAQSYPGETTSPADIPAQPEPIQAPTTWNVLDARLTWRIRPSSDARVSFGAICAVSGQVFWRFRKHVERQHEKQARKEVQIRWQLQGQLKPGMQNWIHAKAGDISQPLDHFNRQERRVFNQVKQWGLSDLAAFRHLITEKYNLTTNHTWICFGGSYPGSLAAWFRLKFPHLVFAAIASSAPVRAVMDYTGYNKVVAASLSNPVVKGSKEQKIKSKQIDTSEXHEIQVILEQLGMPCLQNSQAEIVEELRNSSIGMAFLAMRQWFFQTCTEFGYYQTCEDPDCPFSPRMTLASQLEICSQVYNISYRNVSEGVSFTNDYYGANHPKASRVLFVNGNIDPWYPLSVLKNESDSEPAILINGTSHCANMYSPAPGDPLSLIHARQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXN5</accession>
    <name>A0A6I9YXN5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oxidized purine nucleoside triphosphate hydrolase</fullName>
            <ecNumber evidence="11">3.6.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">2-hydroxy-dATP diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">7,8-dihydro-8-oxoguanine triphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">8-oxo-dGTPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Methylated purine nucleoside triphosphate hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoside diphosphate-linked moiety X motif 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">NUDT1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Also has a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A. Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA. Through this antimutagenic activity protects cells from oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2-oxo-ATP + H2O = 2-oxo-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71395"/>
            <dbReference type="ChEBI" id="CHEBI:172878"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-oxo-dATP + H2O = 2-oxo-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:63212"/>
            <dbReference type="ChEBI" id="CHEBI:77897"/>
            <dbReference type="EC" id="3.6.1.56"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:31584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>8-oxo-dATP + H2O = 8-oxo-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71361"/>
            <dbReference type="ChEBI" id="CHEBI:172871"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:65397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>8-oxo-dGTP + H2O = 8-oxo-dGMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:63224"/>
            <dbReference type="ChEBI" id="CHEBI:77896"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:31576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(6)-methyl-ATP + H2O = N(6)-methyl-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
            <dbReference type="ChEBI" id="CHEBI:172873"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:67609"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>N(6)-methyl-dATP + H2O = N(6)-methyl-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:169976"/>
            <dbReference type="ChEBI" id="CHEBI:172872"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:67605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O(6)-methyl-dGTP + H2O = O(6)-methyl-dGMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67600"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:169974"/>
            <dbReference type="ChEBI" id="CHEBI:169975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:67601"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.56" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013928794.1">
        <property type="nucleotide sequence ID" value="XM_014073319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXN5"/>
    <dbReference type="GeneID" id="106554607"/>
    <dbReference type="KEGG" id="tsr:106554607"/>
    <dbReference type="CTD" id="4521"/>
    <dbReference type="OrthoDB" id="408303at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008413">
        <property type="term" value="F:8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008828">
        <property type="term" value="F:dATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042262">
        <property type="term" value="P:DNA protection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03427">
        <property type="entry name" value="NUDIX_MTH1_Nudt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003563">
        <property type="entry name" value="8ODP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43758">
        <property type="entry name" value="7,8-DIHYDRO-8-OXOGUANINE TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43758:SF2">
        <property type="entry name" value="OXIDIZED PURINE NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01403">
        <property type="entry name" value="8OXTPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="22">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00053094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013928794.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20879" checksum="E5293B3A1E7CB221" modified="2020-10-07" version="1">MLTTKLYTLVLVVQPQRILLGMKKRGFGAGLWNGFGGKVQLGETVEQAARRELQEESSLIVDTLQKMGQITFEFVGNAELMEVHIFRADSFHGEPTESDEMRPQWFGVDKIPFKEMWPDDIYWLPLLLQKKYFLGYFKFRGQETILEYTLKEVEKMEEGTGGGFPDGGRIKDILAGKEPLPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYG1</accession>
    <name>A0A6I9YYG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GCSH</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="function">
        <text evidence="6">The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST). Has a pivotal role in the lipoylation of enzymes involved in cellular energetics such as the mitochondrial dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT), and the mitochondrial dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (DLST).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="8">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929347.1">
        <property type="nucleotide sequence ID" value="XM_014073872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYG1"/>
    <dbReference type="GeneID" id="106555097"/>
    <dbReference type="KEGG" id="tsr:106555097"/>
    <dbReference type="CTD" id="2653"/>
    <dbReference type="OrthoDB" id="10264154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000045">
        <property type="entry name" value="Glycine cleavage system H protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="5 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="75"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="7">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929347.1"/>
        </source>
    </evidence>
    <sequence length="182" mass="19413" checksum="FF804A3B207D0950" modified="2020-10-07" version="1">MAVAATAAAWRALRRVGLGAAAGPRGSLLPWPSSSSGGGIVRSPEARRLSTSPALLLAARRFTDKHEWTAVENGIGTIGISNFAQEALGDVVYCSLPEIGTKLNKQDEFGALESVKAASEIYSPITGEVTEINTALADNPGLVNKSCYQDGWLIKMTVDNPSELDDLLTEDAYEKYIKSIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYR4</accession>
    <name>A0A6I9YYR4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106554852</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929061.1">
        <property type="nucleotide sequence ID" value="XM_014073586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYR4"/>
    <dbReference type="GeneID" id="106554852"/>
    <dbReference type="KEGG" id="tsr:106554852"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01224">
        <property type="entry name" value="PH_Collybistin_ASEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000055">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000018">
        <property type="entry name" value="Phosphatidylinositol-3,4, 5-trisphosphate-dependent Rac exchange factor 2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000094">
        <property type="entry name" value="Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051832">
        <property type="entry name" value="mTOR-Rac_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829">
        <property type="entry name" value="DEP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829:SF6">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE-DEPENDENT RAC EXCHANGER 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="40"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="262"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="5">
        <location>
            <begin position="411"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="5">
        <location>
            <begin position="519"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929061.1"/>
        </source>
    </evidence>
    <sequence length="589" mass="67555" checksum="DB97BBA72B7E04EA" modified="2020-10-07" version="1">MEVQSATGSPSVAAATTAAAPGPGAPPSEPAKDTDRQLRLRLCVLNEILGTERDYVGTLRFLQSAFLHRIRQNAVDKAEKYITEENVKILFSNMEDILDVHNDFLTALEYCLHPEPHSQHELGDVFLKFKDKFIVYEEYCSNHEKALRLLMELNKIPPVRQFLLSCMLLGGRKTTDIPLEGYLLTPIQRICKYPLLLKELAKRTPTKHPDYQAVLNALQAMKTVCSNINETKRQMEKLEALEQLQSHIEGWEGSNLTDISTQLLLQGNLLKISAGNIQERTFFLFDNLLVYCKRKSRVAGKKTSKRTKSINGSLYIFRGRINTEVMEVENVEDGTADYHSNGYTVTNGWKIHNTAKNKWFVCMAKTAEDKQKWLDAIIKEREQRESLKLGMERDAYAMIAEKGEKLYHMMTNKKVNLIKDRRRKLSTVPKCFFGNEFVCWLKEIGEISKPEEGVNLGQALLENGIIHHVADKHHFKNEQVMYRFRYDDGTYKPRSELEDIMSKGVRLYCRLHSLYTPVIKDRDYHLKTYKSVIPANKLVDWLIAQGDCQTREDAVALGVGLCNNGFMHHGKPLILPYPNQLSRILTLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYU8</accession>
    <name>A0A6I9YYU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metaxin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial outer membrane import complex protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MTX2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport of proteins into the mitochondrion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with MTX1/metaxin-1. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929492.1">
        <property type="nucleotide sequence ID" value="XM_014074017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYU8"/>
    <dbReference type="GeneID" id="106555230"/>
    <dbReference type="KEGG" id="tsr:106555230"/>
    <dbReference type="CTD" id="10651"/>
    <dbReference type="OrthoDB" id="198787at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03211">
        <property type="entry name" value="GST_C_Metaxin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000033">
        <property type="entry name" value="metaxin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF38">
        <property type="entry name" value="METAXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01180">
        <property type="entry name" value="SUF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="14">
        <location>
            <begin position="138"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013929492.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="24061" checksum="9F43A10DE4578C64" modified="2020-10-07" version="1">MCNLPVQVVCRANAEYMSPSGKVPFIHVGNQVVSELGPIVQFVKAKGHSLSDGLDEVQKAEMKAYMELVNNMLLTAELYLQWCDDATVQEITHPRYGSPYPWPLNRILAYQKQWEVQRKMKAVGWGNKSLDQVLEDVDQCCQALSQRLGTQLYFFNKKPTELDALVFGHLFTILTTQLISDELLEKVKGYRNLIAFCKRIEQHYFEVKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYV1</accession>
    <name>A0A6I9YYV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Histamine H3 receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106554989</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929224.1">
        <property type="nucleotide sequence ID" value="XM_014073749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYV1"/>
    <dbReference type="GeneID" id="106554989"/>
    <dbReference type="KEGG" id="tsr:106554989"/>
    <dbReference type="OrthoDB" id="10071887at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004969">
        <property type="term" value="F:histamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003980">
        <property type="entry name" value="Histamine_H3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF195">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01471">
        <property type="entry name" value="HISTAMINEH3R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="41"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013929224.1"/>
        </source>
    </evidence>
    <sequence length="401" mass="46442" checksum="C8B051D20F15EB5B" modified="2020-10-07" version="1">MKSCLIPMRLYHFKCPGSERLRVWWNHCLSLKLPTIWPQLMAVSPTLFPLLPGGFCIPLYIPYILTDEWKFGRGLCKLWLVVDYLVCTASGFNIVLISFDRFISVTRAVSYRVQKGMTKSTAAKMVMVWIAAFLLYGPAIISWEYIAQQSIIPENKCYAEFHYNWYFLMIASTIEFFTPFISVTYFNLRIYFNIRKRTPVRSESSSEARKDCERFCCRKKREHVVAFTKRENKRNEKVSSLTRSKPAVGKLGTHDLQTPSVELNINQDFPPWQVDIQTKSPWDGVYKALENLSGTMREAEVANTAASRFRLSRDKKIAKSLAIVVCIFAVCWAPYTLLMIIRAVCHGRCIHHDLYEVSFWLLWLNSAINPVLYPLCHISFRKAFLKLLCPAKAKIHPNVFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z167</accession>
    <name>A0A6I9Z167_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MINK1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013930753.1">
        <property type="nucleotide sequence ID" value="XM_014075278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z167"/>
    <dbReference type="GeneID" id="106556279"/>
    <dbReference type="KEGG" id="tsr:106556279"/>
    <dbReference type="CTD" id="50488"/>
    <dbReference type="OrthoDB" id="8957712at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CNH" evidence="7">
        <location>
            <begin position="231"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013930753.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="46804" checksum="231DD4AFFBEF2596" modified="2020-10-07" version="1">MQQNFVLLKERPEEAAPKPLKKALDYSSSSEEADSSEEEEEEEEEEEAGEGERSEPGASTPGARDGDTDSVSTMVVHDVEDLTGGSGGSESSYGEGTMLVQRTPEEERGLLHSDSNGYTNLPDVVQPSHSPTEAKSSNGSSSPAKDGATDYQSRGLVKAPGKSSFTMFVDLGIYQSSPGGGDTIPITAFDPGRLEQLQLEARKGSVVNVNPTNTRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYSLINRRRFQQMDVLEGLNLLTTISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCIHYRVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVELTVEEGQRLKVIYGSCAGFHAIDVDSGNNYDIYIPVHFACICI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1R6</accession>
    <name>A0A6I9Z1R6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interferon-inducible GTPase 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106556523</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930988.1">
        <property type="nucleotide sequence ID" value="XM_014075513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1R6"/>
    <dbReference type="GeneID" id="106556523"/>
    <dbReference type="KEGG" id="tsr:106556523"/>
    <dbReference type="OrthoDB" id="422720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF17">
        <property type="entry name" value="IRG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="68"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013930988.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="37397" checksum="82920F0663F4FBE1" modified="2020-10-07" version="1">MPDIDEKHPNVSFPFQIIFTGQRATMGNKITKDFLSKQFEEFGRDLSQSSVPELAAKYQKDLNEMQNLPLNIAVAGSVGAGKSSFVNAIRDVRDDDEEAAETGIVEQTKDPKAYPHPSSPQIQIWDLPGIGTDNFKAADYLKKIQFETYDVFIMVTSDRFTENDALLAEEIHRTKKKFYYVRSKIDISIESEKRKRNFDMEETLAKIRRYCEDSLSKAGDLSARVFLISNWKVDKYDFPRLQETIAAELPDYKKQILTLAVHHFSEKELAVKKELMKSYIQKVALVSCACGIVRVPGLSMFCEIGILLDALKRICIAFGLDDQSLHFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4J2</accession>
    <name>A0A6I9Z4J2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106556697</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931144.1">
        <property type="nucleotide sequence ID" value="XM_014075669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4J2"/>
    <dbReference type="GeneID" id="106556697"/>
    <dbReference type="KEGG" id="tsr:106556697"/>
    <dbReference type="OrthoDB" id="8934037at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990349">
        <property type="term" value="P:gap junction-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF46">
        <property type="entry name" value="GAP JUNCTION BETA-2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="5" id="KW-1009">Hearing</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="139"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013931144.1"/>
        </source>
    </evidence>
    <sequence length="224" mass="26192" checksum="62B7A0BD69165239" modified="2020-10-07" version="1">MDWGALQAILGGVNRHSTSIGKIWLTVLFIFCVMILVVAAEKVWGDEQQDFTCNIKQPGCENVCYDEHFPISHIRLWALQLIFVSTPALLVAMHVAYRRHKEKKECRKMDIVDRKTQRFHIEGPLWWTYTTSIFFRIIFEVAFTYIFYYIYRGYQMPRLVKCTDFPCPNTVDCFISRPTEKTVFTVFMLAASGICMFLNLLELSYLVIKFCMKKSRGVNSLPNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4P0</accession>
    <name>A0A6I9Z4P0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Epithelial splicing regulatory protein 1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106556727</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESRP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931174.1">
        <property type="nucleotide sequence ID" value="XM_014075699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4P0"/>
    <dbReference type="GeneID" id="106556727"/>
    <dbReference type="KEGG" id="tsr:106556727"/>
    <dbReference type="OrthoDB" id="431068at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12736">
        <property type="entry name" value="RRM1_ESRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12739">
        <property type="entry name" value="RRM2_ESRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12742">
        <property type="entry name" value="RRM3_ESRP1_ESRP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000041">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000070">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000056">
        <property type="entry name" value="epithelial splicing regulatory protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034427">
        <property type="entry name" value="ESRP1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="225"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="326"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931174.1"/>
        </source>
    </evidence>
    <sequence length="636" mass="70939" checksum="E021890178BF53C9" modified="2020-10-07" version="1">MTTSPDYLVILFGVTAGATGARLGSDERELIQLLWRVVDLANKKVGNLHHVLIGPDRLSLTDECREETGLDEDILALAPQLEQALGQFNQSVSNELNIGVGTSFCLCADGQLHIRQVLHPEASKKNILLPECFYSFFDLRKEFKKCCPGSPEIDKLDAGSMIQYLNPDKRSSAQSFGMQQLEDMGNVILALISEAYNHRFSEPERVNYKFESGTCSKMELVDDNTVIRARGLPWQSSDQDIARFFKGLNIAKGGAALCLNAQGRRNGEALVRFVSEEHRELALQRHKHHMGNRYIEVYKATGEDFLKIAGGTSNEVAQFLSKENQVIVRMRGLPFNVTSEEVLAFFGQHCPVTGGKEGILFVTYPDNRPTGDAFVLFACEEYAQNALKKHKDLLGKRYIELFRSTAAEVQQVLNRYSSAPLIPLPTPPILPVLPQQFVPPTTVRDCVRLRGLPYAATIEDILGFLGEFSGDIRTHGVHMVLNHQGRPSGDAFIQMKSSDRAFLAAQKCHKKTMKDRYVEVFQCSAEEMNFVLMGGTLNRNGLSPPPCKLPCISPPSYSFPTPTAIIPTEAALYQPSLFLNPRTLQPSTAYYPAGTQLFMNYTTYYPSMQQRMDLYSQMGRPGHCPKNGYLFKGPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4Z5</accession>
    <name>A0A6I9Z4Z5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Ubiquitin-related modifier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">URM1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the URM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931046.1">
        <property type="nucleotide sequence ID" value="XM_014075571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4Z5"/>
    <dbReference type="GeneID" id="106556585"/>
    <dbReference type="KEGG" id="tsr:106556585"/>
    <dbReference type="CTD" id="81605"/>
    <dbReference type="OrthoDB" id="10248987at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01764">
        <property type="entry name" value="Ubl_Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000021">
        <property type="entry name" value="Ubiquitin-related modifier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03048">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14986">
        <property type="entry name" value="RURM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037379">
        <property type="entry name" value="Ubiquitin-related_modifier_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="modified residue" description="1-thioglycine" evidence="6">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)" evidence="6">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013931046.1"/>
        </source>
    </evidence>
    <sequence length="91" mass="10567" checksum="540775C9FFDB58FD" modified="2020-10-07" version="1">FRGGAELLFDGVRKHQVNLPHQSEPWKLRHLLTWIKENLLKERPELFVQGDSVRPGILVLINDADWELMGNLDYQLQDRDHVVFISTLHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z565</accession>
    <name>A0A6I9Z565_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA topoisomerase 2-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TOP2B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931349.1">
        <property type="nucleotide sequence ID" value="XM_014075874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z565"/>
    <dbReference type="GeneID" id="106556853"/>
    <dbReference type="KEGG" id="tsr:106556853"/>
    <dbReference type="CTD" id="7155"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012542">
        <property type="entry name" value="DTHCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08070">
        <property type="entry name" value="DTHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Tc1-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="DTHCT" evidence="3">
        <location>
            <begin position="388"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931349.1"/>
        </source>
    </evidence>
    <sequence length="502" mass="56809" checksum="43BD210B1054CC70" modified="2020-10-07" version="1">MTCRAGTNVTKGTISNTLRHQGLRSCSARCVPLLKPVHIWAHLKFAREYLDDPEEDWENVTWSDETKVELFGRNTTRRVWRRENADLHQKNIIPTMKHRGGNIMLWGCFSIKGPGRLIHRRIVPQITSAMKADASKKLLKKKKGDLDTSAIKLEFDEEFGGTPIEGAGEELLNTPGPVIKTPKPKREKKEPGTRVRRTPSATKTSAKKVKKRNPWSDDETKSESDLEENDPVIIPRHSLLRRAAAERPKYTFDFSEEEEDADDDEDANNNDLNELKVKTSPNINDREDEFVPSDSLEKDEYDFSPIKTKPSSEKVSQDKTNQDFGNLFTFPSYSRKANIDTSKFDSDEEDSTPVFSSTFIPKQTETLSSKTVVTKKAKLSSDMPSKLKRSPKPKKMELINSDSESEFGIPKKSIVPKGKGRGAKKRKISGSENEGEYNPGRKSPKSIPSKKLKKTAFDQDSDVDILPSDFASETTSRPRTGRARKEVKYFAESDEDDDFPMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5J1</accession>
    <name>A0A6I9Z5J1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">SPRY domain-containing SOCS box protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">LOC106557252</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPSB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931921.1">
        <property type="nucleotide sequence ID" value="XM_014076446.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931922.1">
        <property type="nucleotide sequence ID" value="XM_014076447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5J1"/>
    <dbReference type="GeneID" id="106557252"/>
    <dbReference type="KEGG" id="tsr:106557252"/>
    <dbReference type="OrthoDB" id="5547302at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03743">
        <property type="entry name" value="SOCS_SSB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12906">
        <property type="entry name" value="SPRY_SOCS1-2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000007">
        <property type="entry name" value="SPRY domain-containing SOCS box protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF3">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="34"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="6">
        <location>
            <begin position="221"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013931921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013931922.1"/>
        </source>
    </evidence>
    <sequence length="273" mass="31146" checksum="48DA6F8E35F5E550" modified="2020-10-07" version="1">MGQKISGSIKSVDVRDPPYRPVKRELRGPDFCKPARLDMLLDMPPAHLEIQYKHAWNNEDRSLNIFVKEDDRLTFHRHPVAQSTDCIRGKVGYTRGLHVWQIHWPTRQRGTHAVVGVSTAEAPLHSVGYTSLVGSNNESWGWDLGRNKLYHNCKNQPGVTYPVFLEPDESFVLPDSLLVVLDMDEGTLSFIVDGQYLGVAFRGLKGKKLYPTVSAVWGHCEITMRYVNGLDPEPLPLMDLCRRSIRFALGRERLHDIESLPLPQSLKNYLQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5T3</accession>
    <name>A0A6I9Z5T3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methionine aminopeptidase 2</fullName>
            <shortName evidence="11">MAP 2</shortName>
            <shortName evidence="11">MetAP 2</shortName>
            <ecNumber evidence="11">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Initiation factor 2-associated 67 kDa glycoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Peptidase M</fullName>
            <shortName evidence="11">p67</shortName>
            <shortName evidence="11">p67eIF2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 16" type="primary">METAP2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 12">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="function">
        <text evidence="10 11">Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11 12">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Binds EIF2S1 at low magnesium concentrations. Interacts strongly with the eIF-2 gamma-subunit EIF2S3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="11">About 30% of expressed METAP2 associates with polysomes.</text>
    </comment>
    <comment type="PTM">
        <text evidence="11">Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013931549.1">
        <property type="nucleotide sequence ID" value="XM_014076074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5T3"/>
    <dbReference type="GeneID" id="106556992"/>
    <dbReference type="KEGG" id="tsr:106556992"/>
    <dbReference type="CTD" id="10988"/>
    <dbReference type="OrthoDB" id="7848262at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000106">
        <property type="entry name" value="Methionine aminopeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000003">
        <property type="entry name" value="Methionine aminopeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="14">
        <location>
            <begin position="172"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013931549.1"/>
        </source>
    </evidence>
    <sequence length="481" mass="53491" checksum="BAA3F07E99EE60EB" modified="2020-10-07" version="1">MAGVEQEAVPVEEKHLNGELLLEPEERESAGGAAADENAKKKRKKKKKSKGGAVGQQETEKELEATLDEVANQLDKQTLEEKDKDDDDEDGEGDGDSAAGKKKKKKKKKKGPKVQTDPPSIPICDLFPSGIFPKGEECEYPATQDGRTAAWRTTSDEKRALDQANEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIRENGLNAGLAFPTGCSLNNCAAHYTPNAGDPTVLQYDDICKIDFGTHINGRIIDCAFTVTFNPKYDKLLQAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGPYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRAKHLLNVVNENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPMCKEVVSRGDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z636</accession>
    <name>A0A6I9Z636_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Shugoshin-like 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106557415</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shugoshin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932137.1">
        <property type="nucleotide sequence ID" value="XM_014076662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z636"/>
    <dbReference type="GeneID" id="106557415"/>
    <dbReference type="KEGG" id="tsr:106557415"/>
    <dbReference type="OrthoDB" id="5990092at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051177">
        <property type="term" value="P:meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038889">
        <property type="entry name" value="Shugoshin1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21577">
        <property type="entry name" value="SHUGOSHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21577:SF3">
        <property type="entry name" value="SHUGOSHIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="367"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="708"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="924"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="598"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="935"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013932137.1"/>
        </source>
    </evidence>
    <sequence length="945" mass="105841" checksum="58210755D7495357" modified="2020-10-07" version="1">MSFQECAETSSMFFKNGVRERMKEKKNGVLKAAKMNASLASKIRTRTINNSSIIKVSLKQNNKALAMALNAAKITAKKLTDEKMRLQKEVELGHFENACLRHKLSSVKKYIDELQLFMNSCFQTAIKMTRFSESDLYSSLLEERGHNDTHDISCSQDDSNCPRVAPKFMRIPLSHVDDEGSGNARKTSPSLEKFPLDPLESAFGETWGSLPFHEVKKSRSSFTDKVLASSGNSHAGLHGVDDSTLALNSSTNFGVIILDNLWNVPQNSRSNSLSLLDKNYPVHQCKDTARSHSDSILSEHVTKRKKRKTGISATFGNLEAELSDIDKLKGTQEAESLIKDNLEISQVGELITLSTKSESYIKYKAKERETPEKDTAKTKNTMFSTSQSKLSLKNDNNGRPDVNAAVSKTSELNSSEEKLPTSVCHMENLKQDRKEKCSSQNLGQTRPKRRTFVVEPNCINNSTTQEIEKGILYLEKIKNLENQAKRPESGSTSNDVQQNETSCSYSTKCLNKSKNCRRTTVLDPGPSNDKDCCASFQPIKEKTDLENLDSLETRFQNPESNSLNTVNTELSVGFQTQIPSQGAILGLNVKRKSKKKVHENNESHRLSEMEEQSLGNIDSELPQSEIKKIKQREKTMNFDRDTIQKEHSSANADVFDLNEKAQKASKKKRKSKCTPSFFPISLDDEDNFFEGSNSSVKKNWETSTALNSFSSTKKRGHSEKTNWTANSNEAGLKKKRTKFSKKTCIRNDLGNKGTDCEPGPEEYEITPTSKTKWDFLQVPGGSTLQKPTVEITKTLPLNPDSPSVGLSKSPSYVEVQESPERDCALSIPSTVLKKNIKQLECSEKEEMPPIQAIPETSPEQENGNKILKDLTNSISLEASPICLSRRRKKEVSYAEPRLNKKLRRGDPFTMTDFLSSPIYKTKKKKLTKGAEMSRKTKKIKTANSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6Q8</accession>
    <name>A0A6I9Z6Q8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 8-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106557022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013931596.1">
        <property type="nucleotide sequence ID" value="XM_014076121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6Q8"/>
    <dbReference type="GeneID" id="106557022"/>
    <dbReference type="KEGG" id="tsr:106557022"/>
    <dbReference type="OrthoDB" id="421121at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21341">
        <property type="entry name" value="TTC8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000168">
        <property type="entry name" value="tetratricopeptide repeat protein 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000169">
        <property type="entry name" value="tetratricopeptide repeat protein 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028796">
        <property type="entry name" value="BBS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44177">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44177:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="350"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931596.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="57359" checksum="C98B53406EAEBE37" modified="2020-10-07" version="1">MGMEMDPLLHALSYFRRRKFQLCSDLCSQVLEKEPGDQAAWCLKMRALTEMVYVDEIEVDQEGIAEMMLDENAIAQVARPGTSLKMPGTGNGAGPSKAIRPVTQTGRPLTGFVRPSTQAGRPGSIEQALKTPRTAHTARPMTSASGRYVRLGTASMLTNPDGPFINISKLNLNNYAQKPKLAKALFEYIFHHESDVKNALDLAAYATQYAQFKDWWWKVQIGKCYYRLGLYREAETQFKSALRQQYMVDTVLYLAKVYSRLDQPLTALNVFKQGLERFPGEVFLLCGMARIHEEMNNISTAAEYYKDVLKQDNTHVEAIACIASNHFYSDQPEIALRFYRRLLQMGIYNSQLFNNLGLCCFYAQQYDMILSSFERALSLAENEEQAADVWYNLGHVAVGIGDRNLAHQCFKLTLANNNDHAEAYNNLAVLEMQKGHIEQARVFLQTASSIAPHMYEPHFNFAVLSEKVGDIQRSYIEAQKSKEIFPEHVDSQQLINQLKQHFARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z847</accession>
    <name>A0A6I9Z847_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mevalonate kinase</fullName>
            <ecNumber evidence="3">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MVK</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="8">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013931960.1">
        <property type="nucleotide sequence ID" value="XM_014076485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z847"/>
    <dbReference type="GeneID" id="106557278"/>
    <dbReference type="KEGG" id="tsr:106557278"/>
    <dbReference type="CTD" id="4598"/>
    <dbReference type="OrthoDB" id="1652964at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000119">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="9">
        <location>
            <begin position="120"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013931960.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="25372" checksum="E6DBB4676F7754B8" modified="2020-10-07" version="1">MWKEGSLVLSAPGKVILHGEHAVVHGKVALATALDLRTFLRMTPRQDGRISLQLLNLGSKLSWEAVELQPLLAAFSAELPEGQPPSFEQVEKLKEFAGDDNGHKAIQSVAAAAFLYLYLSVASKYGVLPTVDILVWSELPTGAGLGSSAAYSVCLAGALLSGCGAVRYPLQEGQEAARWTKEELDVINKLAFQGEQVIHGNPSGVDNAVSTWGGALRYISGKISPLKSVPTLRILLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z8L8</accession>
    <name>A0A6I9Z8L8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc-binding protein A33-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106557407</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Neurotoxin that produces dose-dependent hypolocomotion and hyperalgesia in mice. May directly act on the central nervous system, as it is 6500-fold more potent when administered intracerebroventricularly than intraperitoneal.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ohanin/vespryn family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932120.1">
        <property type="nucleotide sequence ID" value="XM_014076645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z8L8"/>
    <dbReference type="GeneID" id="106557407"/>
    <dbReference type="KEGG" id="tsr:106557407"/>
    <dbReference type="OrthoDB" id="128536at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0528">Neurotoxin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0800">Toxin</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="292"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="208"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013932120.1"/>
        </source>
    </evidence>
    <sequence length="490" mass="55906" checksum="9FD8FCACF7C13A29" modified="2020-10-07" version="1">MAAPTGIERFAEDLRCSICLELFSDPVILECGHNYCQACITRFWAEIPNNHEEDMPPTLPTCPECRREIPGGKYTPNRVLGQLACKAQESLLVQAGDEDTNGDKDDEEEMQGEKLFCTEDGCLARALQPDHWGHQCLPLIEAVEHFKGILTTSQAQLESKIQAERSVEEQRAQKPPEITAQRLRLEQNVSAQFMELHQWLQEKEAALIREIQREEKLLLSELDRNQRNSQEQIRTANEQIAKIQARLAMEPDSESFLKDIKGFVERYCPGEDKTSPFPVISRDFNLGQFKGPIQYMVWREMLLALRPFPSYITLDPATNHPNLMLSKDLDTVRLEDNPEEVVPDGPERFSKTVCVLGAQGFTCQCHYWEVEVGNKTSWDIGVAKESVNRKEAKVTVKPSNGFWAIWLRNGNEYKALDSPSKQLLLKVKPQRVGVYLDYEGGQVSFYDADTMTHIYTFLDDFYECLYPMFSPGVNKDGLNGEPLCLLHPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZX6</accession>
    <name>A0A6I9WZX6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-binding protein EWS-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106538551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908534.1">
        <property type="nucleotide sequence ID" value="XM_014053059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZX6"/>
    <dbReference type="GeneID" id="106538551"/>
    <dbReference type="KEGG" id="tsr:106538551"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013908534.1"/>
        </source>
    </evidence>
    <sequence length="88" mass="9513" checksum="5BD6A8C82E20DAE6" modified="2020-10-07" version="1">MASTDYSTYTQAAAQQGYTAYAAQPTQGYAQTTQQAYGQQSYGTYGQPTDANYTQAQTTATYGQTAYAGSYGQPPTGEHLCLRSLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0A1</accession>
    <name>A0A6I9X0A1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cadherin-24-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106537985</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907791.1">
        <property type="nucleotide sequence ID" value="XM_014052316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0A1"/>
    <dbReference type="GeneID" id="106537985"/>
    <dbReference type="KEGG" id="tsr:106537985"/>
    <dbReference type="OrthoDB" id="6079678at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF272">
        <property type="entry name" value="CADHERIN-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="41"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="122"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="231"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013907791.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="35659" checksum="9F7EE8563433B41A" modified="2020-10-07" version="1">MRMWWLPSQLSSRESKKPRAPSEAQKKRRQQAEARESKLHTDVDHGEGRIKYVLTGEGAGTVFVIDEKTGNIHVTKTLDREEKAQYTLLAQAVDRASNRPLEPPSEFIIKVQDINDNPPVFLQGPYHATVPEMSNVGTSVIQVTAHDADDPTYGNSAKLVYTVLEGLPFFSVDPQTGVIRTATPNMDRETQQEFLVVVQAKDMGGHTGGLSGSTTVTVTLSDVNDNPPRFPQSSYQFSVVETAPPGSAVGRLRAHDPDEGENALLAYSILEGGDGAEAFAIHTDMLGQDGIITLKKVGEAPQKYGVLLSFFLLGALIWEPLSIHPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X194</accession>
    <name>A0A6I9X194_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LGALS8</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908111.1">
        <property type="nucleotide sequence ID" value="XM_014052636.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908112.1">
        <property type="nucleotide sequence ID" value="XM_014052637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X194"/>
    <dbReference type="GeneID" id="106538202"/>
    <dbReference type="KEGG" id="tsr:106538202"/>
    <dbReference type="CTD" id="3964"/>
    <dbReference type="OrthoDB" id="6251307at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000023">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000054">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF22">
        <property type="entry name" value="GALECTIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="18"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="167"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013908111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013908112.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="34253" checksum="E2455188EE87FAFD" modified="2020-10-07" version="1">MMSLDNFDRTVRSPVVPYAGTLMDGLSPGDLVIIHGFVPEDSERFQVDFQCGNSAKPRADVAFHFNPRFKRSGCLICNTLQKEKWGTEEITYEMPFEKTKPFEIVFMVRHDKFQVSVNRKHVLLYKHKINLEKIDTLAISGKVKVTLIGFVSNKEDVPDSSQFVIPYSAKLNYSVEPGRTIAVKGEVKEKANGFAINLKPSESSDIALHLNPRIKDNVFVRNSYLFDSWGEEERSIAHFPFRPGMYFELLIFCKDHQYDVAINGVHILEYKHRFKQLDKIRILEVFGDVQLLDVRCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1Q0</accession>
    <name>A0A6I9X1Q0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106538334</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908266.1">
        <property type="nucleotide sequence ID" value="XM_014052791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1Q0"/>
    <dbReference type="GeneID" id="106538334"/>
    <dbReference type="KEGG" id="tsr:106538334"/>
    <dbReference type="OrthoDB" id="6151005at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15227">
        <property type="entry name" value="7tmA_OR14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000037">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="40"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013908266.1"/>
        </source>
    </evidence>
    <sequence length="320" mass="36292" checksum="FDD49B5CA9190C84" modified="2020-10-07" version="1">MDNVTTVPSYFLLLEFSKTRQLQLLHFFSFLVFYFATIIGNLLIIFAITFDYQLHTPMYFFLMNLAVQDLGLISVIVPKSMINSLKDTRCISYFGCVAQVFLFVSFVASDFFLLTAMAYDRYVAICQPLHYEMVMNWKTCTEIMILVWVSSLLYGMLHTSGTFSVHYCSNIVNQFFCEIPYLLNLSCSGFNLVEVGILVASAFGGLGCFIFIIVSYVIIFKTVWKIPSVQGRQKAFSTCAPHLIVVSMFTITVIFAYLRPASNSTSYLDLMLTVLYSILPPLFNPIIYSMRNKKILSKLGMILIGKLTQTNHYVVSGTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2T7</accession>
    <name>A0A6I9X2T7_9SAUR</name>
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        <submittedName>
            <fullName evidence="3">GS homeobox 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106538235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908152.1">
        <property type="nucleotide sequence ID" value="XM_014052677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2T7"/>
    <dbReference type="GeneID" id="106538235"/>
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    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GSH1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421">
        <property type="entry name" value="GS HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421:SF1">
        <property type="entry name" value="GS HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013908152.1"/>
        </source>
    </evidence>
    <sequence length="216" mass="21669" checksum="5D6E1D5B67830271" modified="2020-10-07" version="1">MSRSFYVDSLIVKDAAVAARPVVPALPEPPPPPPGQDFLIPLGVPAPLVMSVAGGPACPSRKSGGSFCVCPLCVTSHLHATARAGAAGGGAAAASLPLLKGHFGPGGAGQGGEGQFCPRMSHVQQQTAPQQQQPPLGSVASAAAAAAQALAHPPVCAAAAYGMSDPRRFHCLGLGNAPSMLNASLRLCTMQVCACKGGEDMPKVESGESMNLCRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X496</accession>
    <name>A0A6I9X496_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium/potassium/calcium exchanger 6, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106538992</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="sodium/potassium/calcium exchanger 6, mitochondrial isoform X3"/>
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    </dbReference>
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        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
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    </dbReference>
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        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="526"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="64"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013909131.1"/>
        </source>
    </evidence>
    <sequence length="545" mass="61043" checksum="887B66B8973767C9" modified="2020-10-07" version="1">MTGAFCDLDRCCATLVSLPRQEREGPSDPAVLHTKRHQRRHGNVNQHGEFVISPVEGELRVRKDAELDRENIAFYNLTIEAKDRGLPSLSSTTGIVYVNRPLDRERVSEYRLTITVKDNPENARNARRGLAQDVTMQRENQIGNTDMNSENSHDNCAGIFVTTVVAGGIALVRPFTAASRPFLRDAIFYMIAVFLTFVALYFEQVTLAEALSFLGLYIFYVLTVVVSTWLHKRQRRERFTPPSHPEPELSTDSEEGDSANVNGEEEYQPLLPTEETTFQILTQSINPLDYRKWRRKPCYWKLLKACKLPVELVLLLTVPIVDPDRDDQNWKRPLNCLHLVTGPLICILALKSGTYGLYKFQGVFPVWGLVVLVGATLAAVLFCVTRNDEPPRCHFLFAFLGFFVSAMWINAAATEVVNILQTLGIIFHLSNTVLGLTLLAWGNSIGDMFSDLTMARQGYPRMAFSACFGGIIFNMLVGVGLGCLLQMSSSKWIVRLEPDGLLVWILAGALGLSLVISFLSVPAQCFHLGPVYGYCLIAYYVVFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4G1</accession>
    <name>A0A6I9X4G1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CSNK2A2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013910234.1">
        <property type="nucleotide sequence ID" value="XM_014054759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4G1"/>
    <dbReference type="GeneID" id="106539852"/>
    <dbReference type="KEGG" id="tsr:106539852"/>
    <dbReference type="CTD" id="1459"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905818">
        <property type="term" value="P:regulation of chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF34">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="40"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013910234.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="41229" checksum="DF0B4361D668DF31" modified="2020-10-07" version="1">MPGPAAGSRARVYAEVNGLRSREYWDYEAHVPTWGNQDDYQLVRKLGRGKYSEVFEAINITNNERVVVKILKPVKKKKIKREVKILENLRGGTNIIKLIDTVKDPVSKTPALVFEYINNTDFKQLYQILTDFDIRFYMYELLKALDYCHSMGIMHRDVKPHNVMIDHQQKKLRLIDWGLAEFYHPAQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGQDNYDQLVRIAKVLGTDELYGYLKKYHIELDPHFNDILGQHSRKRWENFIHSENRHLVSPEVLDLLDKLLRYDHQQRLTAKEAMEHPYFYPVVKEQSQPGSDSPVLSSSLTATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X508</accession>
    <name>A0A6I9X508_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calmodulin-regulated spectrin-associated protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106538825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908927.1">
        <property type="nucleotide sequence ID" value="XM_014053452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X508"/>
    <dbReference type="GeneID" id="106538825"/>
    <dbReference type="KEGG" id="tsr:106538825"/>
    <dbReference type="OrthoDB" id="9304414at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF1">
        <property type="entry name" value="CALMODULIN-REGULATED SPECTRIN-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="183"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908927.1"/>
        </source>
    </evidence>
    <sequence length="348" mass="39838" checksum="4EC9F63007098F92" modified="2020-10-07" version="1">HVCFFPPENIPDELREPFYTDQYDQEHIKPPVVNLLLSAELYCRAGSLILKSDAAKPLLGHDAVIQALAQKGLYVTDQEKLVTERDLHKKPIQMSAHLAMIDTLMMAYTVEMISVEKVIACVQQYSSFFQATDLPYDIEDAVMFWINKANEHLKDIMEQEQKLKEQQGAETPGGQKSPSKWFWKLVPARYRKEQTLLKQLPCVPLVENLLKDGRDGCALAALIHFYCPEIVRLEDICLKETMSLADSLYNLQLIQEFCQEYLNQCCHFALEDMLYAASSVKSNYLVFMAELFWWFEVVKPSFVQPRAVGHPQADPPMEASSVPPTNVNQRSYPGVLHDSDLITRYQPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5A8</accession>
    <name>A0A6I9X5A8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 Z</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme Z</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin carrier protein Z</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin-protein ligase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106538888</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013909002.1">
        <property type="nucleotide sequence ID" value="XM_014053527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5A8"/>
    <dbReference type="GeneID" id="106538888"/>
    <dbReference type="KEGG" id="tsr:106538888"/>
    <dbReference type="OrthoDB" id="47801at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF26">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="16">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="144"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013909002.1"/>
        </source>
    </evidence>
    <sequence length="173" mass="19786" checksum="640205E3CB1D0D69" modified="2020-10-07" version="1">MKISSCFLQSGSTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIQHETIRIAVCDMLEGKYPSPEPLRGVMEKSFMEYYDFYEGVCKERLHLQGQSMQDPFGEKRGHFDYQSLLARLQGIRLKVQEKHQQENPDIDSESSSSETEMDTQGSIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5N3</accession>
    <name>A0A6I9X5N3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein Shroom4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SHROOM4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shroom family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909132.1">
        <property type="nucleotide sequence ID" value="XM_014053657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5N3"/>
    <dbReference type="GeneID" id="106538993"/>
    <dbReference type="KEGG" id="tsr:106538993"/>
    <dbReference type="CTD" id="57477"/>
    <dbReference type="OrthoDB" id="10063560at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014799">
        <property type="entry name" value="ASD2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027685">
        <property type="entry name" value="Shroom_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012">
        <property type="entry name" value="APICAL PROTEIN/SHROOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012:SF35">
        <property type="entry name" value="PROTEIN SHROOM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08687">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51307">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASD2" evidence="7">
        <location>
            <begin position="1085"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="590"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="856"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="984"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1083"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="607"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="877"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="988"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1014"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013909132.1"/>
        </source>
    </evidence>
    <sequence length="1172" mass="128288" checksum="BE1F611B764611B1" modified="2020-10-07" version="1">MIIRRRNVPIIRPHSWHLAKLSEVRTDGTAMTFPADPLSLSWHLGCETSDLPLPWNPLSPHCTTEKNSSLGSMESLDPAGQTYYEGSLSPIDQAMDQSKRDSAYSSFSASSSTSDSALRPEETSSVDGAQPGQLPEPRYLQTGGEPAGLGVRLPSSVQSGSLPRLSRTAAAPPHPPVRQDSLRACNSPLEDPRAPTHAGGLCPENWWNSDISLCSPGRDAHGPGGALATTGPLKDSPFPDQYYLLSSHTEPHLGMKRSPQTSPSSLAESPREPAGRKGHEVELNNCAEPPSSWKAALGGPFGHRHSIPEHLLVAQLQALEVSRGQKAPRWTVSPLHKEQKDPQAPEPCGQKPCEPAEELDSPSTAEEGSPSPRGPCTALGRPNNTLPTRAGSPSLPAFGNGSTSRRDLQAAPCVDPLPEAERSLPAAQKGQHRSAHSRRRSDRFATNLRNEIQWRKAQLQKARGPGGPGCEEEPAPQAEESPAHTAVPAPSPPPLGEGRPLGSSGLPAGVPKRWGSELSVFGEERAPSSPQRTPKEKPVARAGGWGGGRWRWSPEHKLQPQESEPGPSAQLPLEEDGLLPFADRRRFFEETSHPLPARPYGSLQGRLEGRRSPEAEKPGSAEHGTFQRHSLDQSYRCPPSPPVYQDFQPEVLRLCKSLARPGVETEHWWALPCSCAVREACAYSFQEECAVLRSRSMPMPPPSHHAHHCHPCSWSCRSDCCCPGQQKLVEDGGPWHGRKPFQGEFPPDEWEPPAVSRASSHTVSQLLPHKVAFTRVSPFWTCFERTEPAGPSFCRAPSTHSLPWDCEQPKWVAEKGGWEGGLGEPHKPPLRERAYSESHLCTEPARVSAREWREAPLAETQETAPKSPACQTRRRGPPPPRPPPPNWEKYRSARASHQQLLPAQACRPHHVEESWAPGQPGFEAARQRSQSLPGEQLWPNRAQLQCPRLPGAGPVAAPSLPEQDNPHYYCHGSPPRTREWLMAETSDRSVPSRTASSSEGAAAEDPPRVEEQETPTGSPSEQPLTWHPQQGTPVSQDAAGECHRSGPPPSRLNSEELMRDVAGKDRSLAGVLSSGFGLRSAAEMALLEKKRQLTAQLEDAKELQEHVAQREQLVSASVSRCLAPEQLQDYQHFVRMKSALAIQQRQLDDKVKLGEEQLRCLWESLPRRPRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X626</accession>
    <name>A0A6I9X626_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Progestin and adipoQ receptor family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PAQR4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910899.1">
        <property type="nucleotide sequence ID" value="XM_014055424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X626"/>
    <dbReference type="GeneID" id="106540349"/>
    <dbReference type="KEGG" id="tsr:106540349"/>
    <dbReference type="CTD" id="124222"/>
    <dbReference type="OrthoDB" id="535992at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF138">
        <property type="entry name" value="PROGESTIN AND ADIPOQ RECEPTOR FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013910899.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="24950" checksum="7AFB38416DED7879" modified="2020-10-07" version="1">MAGLPPQGQLLPGFCILPAPLPPASAVADGAAAGGDSLACIFPQLGSVLYHLFMNHEGGPAVYHTLLTLDMCGVCMVNTLGALPIIYCTLACCPLLRSIALLAYTGLSSYGIFCAVTARSSVRRLRAFAWQALFRFFFFYLRWVGLGTGHPTSLRSYLIMDGLAFLGGVINISRVPERWKPGCFDYWFNSHQIMHVLVVVSILYLHWGVVADLHWIANSACPKEGPHHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6J8</accession>
    <name>A0A6I9X6J8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Specifically androgen-regulated gene protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6 7" type="primary">LOC106540491</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911083.1">
        <property type="nucleotide sequence ID" value="XM_014055608.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911084.1">
        <property type="nucleotide sequence ID" value="XM_014055609.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911085.1">
        <property type="nucleotide sequence ID" value="XM_014055610.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911086.1">
        <property type="nucleotide sequence ID" value="XM_014055611.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911087.1">
        <property type="nucleotide sequence ID" value="XM_014055612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6J8"/>
    <dbReference type="GeneID" id="106540491"/>
    <dbReference type="KEGG" id="tsr:106540491"/>
    <dbReference type="OrthoDB" id="9898538at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026152">
        <property type="entry name" value="SARG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21555">
        <property type="entry name" value="SPECIFICALLY ANDROGEN-REGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21555:SF0">
        <property type="entry name" value="SPECIFICALLY ANDROGEN-REGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15385">
        <property type="entry name" value="SARG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013911084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013911085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013911086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911087.1"/>
        </source>
    </evidence>
    <sequence length="583" mass="64840" checksum="177553F462B04295" modified="2020-10-07" version="1">MPKKDLWVGIVEPDPTPTVDSAGSCDSVISNNSSSFEMNDGGFDYLTVEEKECLMFLEETLHSLDAEADSGVSADEAEAVELSKLTRTWPTRDVPKELDPDYHGKHKQIDPKGNTSLSGSIPAAILSPGHHSLPKNIMAKNTVHTSKIFVAEAPETNADDLKTLPPLHPTLWDSSKHGTLDILKEVPEIGTRGRISELESVVIPPPEPFQDQRKSHLLIEHESILTDSWKHAENGATSHYESKGNQGVMEEYEARAVKQEFPPEMMETAAGNESFLKSVSPKCNKKSSEQIVNHQEIDHKHILEESQLDSSFKQGPPIAPKPRKLPPNIILKTSKNNVAPLNIDPTHKIKASLPFSGRPRAATGDFSMDKTYALQKEQGRARREALEKLGLPLDNEKNPDDQVIKTSIYSKTKETSYTGSRENVNIDDITLSKKSVQQSDQTDVKEVQPTEISIQRIKQANFKSNTLERSGVGLSSLGTSGSEGENLKNNKRSFLSKITPNFLRNNRMRPASLGTGKDFVDLKENKQHHVEWDKGHERRSYPLQHPSKLPRPACVSVKITPKGDTEEHRKEALKKLGLLKEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X727</accession>
    <name>A0A6I9X727_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein transport protein Sec24B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SEC24B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907193.1">
        <property type="nucleotide sequence ID" value="XM_014051718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X727"/>
    <dbReference type="GeneID" id="106537549"/>
    <dbReference type="KEGG" id="tsr:106537549"/>
    <dbReference type="CTD" id="10427"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000019">
        <property type="entry name" value="SEC24 homolog B, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF42">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC24B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="5">
        <location>
            <begin position="36"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="7">
        <location>
            <begin position="280"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="4">
        <location>
            <begin position="501"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013907193.1"/>
        </source>
    </evidence>
    <sequence length="629" mass="70743" checksum="5064FA96ABAB295E" modified="2020-10-07" version="1">MYNPLTRSYGEPHKRPEVQNSTVEFIASSDYMLRPPQPAVYLFVFDVSHNAIEAGYLEVVCQSLLENLDKLPGDTRTKIGFITFDSTVQFYNLQEGLSQPQMLIVSEIDDIFLPMPDSLLVNLHESKELIKDLLNALPSMFTYTRETHSALGPALQAAFKLMSPTGGRVSVFQTQLPSVGTGYLHSREDPNQRSSTKIVQHLGPATDFYKKLALDCSGQQTAVDLFLLSSQYSDLASLACISKYSAGCVYYYPSFHHIHNPVQKEKLQKDLQRYFTRKIGFEAVMRIRCTKGLSIHTFHGNFFVRSTDLLSLANVNPDAGFAVQMSIEENLTDTSLVCFQTALLYTSSKGERRIRVHTLCLPVVTSLMDVYAGADVQAIICLLANMAVDRSVSSSLSDARDALANAVVDSLSAYMSTASNLQQSALIAPYSLRLFPLYILALFKQKAFRTGTSTRLDDRIYAMCQIKSQPLVHLMKIIYPNLYRIDKLTDEGAIHVNDKVVPQPPLQRLSAEKLTREGAFLMDCGLILYIWLGRSCDNNFIKDVLGYPNYLSVPPKLTQLPELDNVSSERTRSFITWLKDSKSLNPVLQVIKDESPVKTDFLQQLIEDKTEAAFSYYEFLLHIQQQICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X892</accession>
    <name>A0A6I9X892_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DnaJ homolog subfamily B member 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DNAJB12</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910546.1">
        <property type="nucleotide sequence ID" value="XM_014055071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X892"/>
    <dbReference type="GeneID" id="106540076"/>
    <dbReference type="KEGG" id="tsr:106540076"/>
    <dbReference type="CTD" id="54788"/>
    <dbReference type="OrthoDB" id="442087at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000004">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF8">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="109"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013910546.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="41847" checksum="57B2F1A9E5834632" modified="2020-10-07" version="1">MESNRDEAERCIVIGVAAIKANQIEKARRFLEKAQRLYPSQRVGALLDSLTKNKQTADDHSQPKESSNLGSQKRTEDFASANGEAGGDTSKGYTQDQVGAVKRVKQCKDYYEILGVGRDASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGNAYAVLSNPEKRKQYDQFGDAKVSSTRHGHADFNRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYHQRQDRREHQGDGGLGLFVQLMPILILIIVSALSQMMVSSPPYSLSHRPSVGHVFKRTTEHLKVSYYVGDSFAEEYTGTNLKNVERSVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDSDLYQRAQKMGTPSCTRLSEVQASMHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8C0</accession>
    <name>A0A6I9X8C0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106540966</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013911759.1">
        <property type="nucleotide sequence ID" value="XM_014056284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8C0"/>
    <dbReference type="GeneID" id="106540966"/>
    <dbReference type="KEGG" id="tsr:106540966"/>
    <dbReference type="OrthoDB" id="10016527at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051234">
        <property type="entry name" value="TAO_STE20_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TAO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167:SF8">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013911759.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="35247" checksum="3AB2CD409EE6A33F" modified="2020-10-07" version="1">MQEHEQDSELREQMSGYKRMRRQHQKQLMALENKLKTEMDDHRLRLDKDLETQRNNFAAEMEKLVKKHQAAMEKEAKVMANEEKKFQQHIQTQQKKELNSFLESQKREYKLRKEQLKEELSENQSTPKKEKQEWLSKQKENIQHFQAEEEANLLRRQRQYLELECRRFKRRMLLARHNLEQDLVRESVLQRTLWTHYAPRLSKYMRTHEFNLCNQKEGETINQYVAALRKAAAYCEFRDLDELLLDRIICGVRDISLQRRLLTKTNLTLQITLDEARATESLTKSTETLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8X8</accession>
    <name>A0A6I9X8X8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ankyrin repeat and BTB/POZ domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ABTB1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May act as a mediator of the PTEN growth-suppressive signaling pathway. May play a role in developmental processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910202.1">
        <property type="nucleotide sequence ID" value="XM_014054727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8X8"/>
    <dbReference type="GeneID" id="106539828"/>
    <dbReference type="KEGG" id="tsr:106539828"/>
    <dbReference type="CTD" id="80325"/>
    <dbReference type="OrthoDB" id="684045at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18497">
        <property type="entry name" value="BACK_ABTB1_BPOZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18295">
        <property type="entry name" value="BTB1_POZ_ABTB1_BPOZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18296">
        <property type="entry name" value="BTB2_POZ_ABTB1_BPOZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000115">
        <property type="entry name" value="Ankyrin repeat and BTB/POZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000063">
        <property type="entry name" value="Ankyrin repeat and BTB/POZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000064">
        <property type="entry name" value="Ankyrin repeat and BTB/POZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044515">
        <property type="entry name" value="ABTB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB/POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231:SF1">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB_POZ DOMAIN-CONTAINING PROTEIN 1"/>
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</entry>
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    <sequence length="162" mass="18441" checksum="62E77A178AB504D8" modified="2020-10-07" version="1">MDSPKRRVAHRQRDPESSKVEGDSGSQSDVFAKAHEFFRICDVEGKGFIARQDMQRLHGELPLAPEELEKVFNTLDADGNGSLTLEEFTTGFSQFLSERKESFAEVSETGFHPKSKESIAADNEEEEQFSNLVEQLGAQNILKDEDDVKKLWLHLRKHEPHL</sequence>
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    <accession>A0A6I9XA07</accession>
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        </source>
    </evidence>
    <sequence length="1931" mass="207254" checksum="E8B28C5120C3A070" modified="2020-10-07" version="1">MDPQRTAMLGLGSGSEGFSGKSPSAGATGVRREAEVDGSAKEEEEEKRRSREKSASDEAGKEGSLPDAQHQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKQPRRVQKVGKMGEEKNDPGSAKQDPSKLYKKSGEAGPATVESLLAESRARASSPALKKLSPLHPEGLEYAKSAFSTPIGSREPDLKERAQANGPPTVTEKLAQLVSTCPPAKSSKPKQKKPPMTVLSGFAKESGKKLPTPGAAAGTATKDFLKKPISSGTLGLDLVNKELAKKAPGTGGGSGGLISKENGSKKQPGSCPSMAGLTNKDYEKRPSVLSCPVGLVSKEGGKKPLLVTSMAPSGMASKDCGKRLLGTSSPIGLVNRDPGKKSLGMVSPPGLINRDSLKKLPLPGSTVGLFNKDVAKRHLGSDGPVGSVSKDSAKKPPSFGTSSGLINRDSGKKPSAAGPPIGLMAKESSKKHMGVGIMASLTSKDSGKKLMGSTNLVGLAGKDLGALKADTANLPAEPYKRCCGSGPAPLESHVPTGLLKDGAGGSKPFEKHLARQSKGSLPEKLTLQREVSSGGKDGACGQQEISPSERGPSESSKQEKHLPVYCTSPEFRTAGGASEISTAKSPFSAVGEGGLPSPSPTASVATLTHSPPTALSQIPSNPLHSSHMPDLLDSLSEQSSKTPSASDGARSGMNRLGNPGLYTTSKGVVRETSNPNSVCAEESKLGPTPGKKASLVSESNLHASISPSVVSFSSFFNNKPFLKMGAVSSLGKPRQGLEILRGSSSTDSLGKPLKKRKGRKPRWTKACRSPKELELERPELLKNVSCGPLSSSGLDQAKLFEGTGPPDAFAEHDFLKYHLPKLSKSSSLQSLSLLGSAEKEPGKFYSAHVPTSAGCLSDDLFPDMYKSKRNRSKSKEMPHLEGPPKRALKIPASKVFSLQSKEEQEPPVLHPEVEIPSHRQTLAGQAFPKKRGRPKRQIRSGIKMKPPVLSAAPFVTPEKADESEDAGDQPLLPPPSGDFFESHSQLSNTEDNGDSSTGSASDPEGEPEHKEVKRSNGQLVKTIIRKINKMKTLKRKKILNQILSSSADPNKGKVQSKAHTNVSSLAATFSTKLGQQINVSKKGTIYIGKRRGRKPKASFSSLLAAGSNFAILETSGQQASGGMLGQAGAPLLPSGPSSSPEVLPSPVCSQSSGTSGGQSPISSDMSFVEPNSVPCFHLHSRQGSVVQTLAMRKASRARRRLSPPTLFPNSPSHMSELGSLKEATPSPISESHSDETVPSDSGIGTDNNSTSDRAEKFCGPKKRRHSFEHVSLVAPEASAVLGGLKDKHKHKCKRRGHDYLAYDKLKRPKRKRKKKFPQLRGRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSIYSHPSFSLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHGLSFPLSGTGFYPSYGMPYSPSPLAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTASRAPLLSMSTYHPSVPPEMAYSWMLEHKHRHRHKHREHRSSEQPPVSLEALAPASSSRTVLESLKRYRFGKEAAGERYKHKEKHRCHMACPHLSPAKGLLGREEQWARRETAESGPRTLGLQTPLQIDCSDSPPNLPLGRFTPSSEPASSDEHTNLFTSAIGSCRSTGSAGGRKKLSEGSGLFSPQEGSLGRPPRKETLPPIEKVVQGLSGALPGQEKLSQRSVEGTSCSPSRKRSSSESTSSTVVGIPSRGSRISSSSEDPADHLLQRMAHHESQAALDKTLESVLASAPVLPSDSPSRSPAREKSCELLLPGNPGVDGLSLLSGRSPHHHFKRSMVDAMQSQTRRMCSYDKILATKKNLDHVNKILKAKKLQRQSRTGNNFVKRRPGRPRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XA81</accession>
    <name>A0A6I9XA81_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Propionyl-CoA carboxylase beta chain, mitochondrial</fullName>
            <ecNumber evidence="3">6.4.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Propanoyl-CoA:carbon dioxide ligase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PCCB</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:59520"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:60909"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:59521"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23720"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57327"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:23721"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AccD/PCCB family.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013910682.1">
        <property type="nucleotide sequence ID" value="XM_014055207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XA81"/>
    <dbReference type="GeneID" id="106540168"/>
    <dbReference type="KEGG" id="tsr:106540168"/>
    <dbReference type="CTD" id="5096"/>
    <dbReference type="OrthoDB" id="439921at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000017">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit beta 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000016">
        <property type="entry name" value="Propionyl-CoA carboxylase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051047">
        <property type="entry name" value="AccD/PCCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842:SF2">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="9">
        <location>
            <begin position="32"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="10">
        <location>
            <begin position="294"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013910682.1"/>
        </source>
    </evidence>
    <sequence length="537" mass="58673" checksum="9058E75D328BF121" modified="2020-10-07" version="1">MAALRVVPAFLLSRVLRRGCRSFSCSDPRTRAVTVPERIEEKRRAALLGGGQARIDAQHKRGKLTARERVLLLLDPDSFVEYDMFVEHRCADFGMESEKNKYPGDSVVTGQGRINGRLTYVFSQDFTVFGGSLSGAHAQKICKIMDQAVMVGAPVIGLNDSGGARIQEGVESLAGYADIFLRNVLCSGVVPQISLIMGPCAGGAVYSPALTDFTFMVKDTSYLFITGPDVVKSVTNEDVTQEQLGGAKTHTAVSGVAHCAFENDIDALLNLREFFNFLPLSNKEPAPVRECHDPSNRLVHELDTIVPMESTKAYDMLDIVHAVVDEREFFEIMPSYARNLIVGFARMNGRTVGIVGNQPKIASGCLDINSSVKGARFVRFCDAFNIPIITFVDVPGFLPGTAQEYGGIIRHGAKLLFAFAEATVPKITVITRKAYGGAYDVMSSKHLRGDANYAWPTAEVAVMGAKGAVQIIFRGKGAHAEAEYVEKFANPFPAAIRGFVDDIIQPSTTRLRICRDLEVLASKRQSNPWKKHANMPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC78</accession>
    <name>A0A6I9XC78_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lysine-specific demethylase 2B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106540741</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911452.1">
        <property type="nucleotide sequence ID" value="XM_014055977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC78"/>
    <dbReference type="GeneID" id="106540741"/>
    <dbReference type="KEGG" id="tsr:106540741"/>
    <dbReference type="OrthoDB" id="5876800at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15644">
        <property type="entry name" value="PHD_KDM2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000020">
        <property type="entry name" value="lysine-specific demethylase 2B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16866">
        <property type="entry name" value="PHD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CXXC-type" evidence="10">
        <location>
            <begin position="45"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="98"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013911452.1"/>
        </source>
    </evidence>
    <sequence length="275" mass="30630" checksum="4A6B4908B8C5B19D" modified="2020-10-07" version="1">MVGGQPLTTPPSLSLSQNRAVGRPKGKLGPSSAVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQCIAPVLPHTAVCLVCGEAGKEDTVEEEESKFNLMLMECSICNEIIHPGCLKVRESDGVVNEELPNCWECPKCNHAGKTGKAYKQKRGPGFKYASNLPGSLLKEQKINRESKEVVPEAPKRKVECEESPRKKSDERPPKPPPDPLVWKKSSEAAHLRRKRKFEKPQEPAMRKRVRLGNPRFPLGCLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD79</accession>
    <name>A0A6I9XD79_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GTPase-activating protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ARHGAP28</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909113.1">
        <property type="nucleotide sequence ID" value="XM_014053638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD79"/>
    <dbReference type="GeneID" id="106538946"/>
    <dbReference type="KEGG" id="tsr:106538946"/>
    <dbReference type="CTD" id="79822"/>
    <dbReference type="OrthoDB" id="27680at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051497">
        <property type="term" value="P:negative regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04391">
        <property type="entry name" value="RhoGAP_ARHGAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000018">
        <property type="entry name" value="Rho GTPase activating protein 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057323">
        <property type="entry name" value="RHG40/28/18_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF5">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25442">
        <property type="entry name" value="Ubiquitin_RHG40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="166"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00055252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013909113.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="58345" checksum="AF7FB0AF38862AEB" modified="2020-10-07" version="1">MRKKNKHTIRDVRDIFGIHNSSPTFETISVLPVTTNGIDFSVQNPDWKMVSSSDCMEYKLNKGSQLEKEELSLEISFSESITVPPKHRECHKSQRLKKDDSALPKYTIRKSRLGLTEVGDLSPEDMKKIRYLSLIELTAVYDALGIELKRNRTERMKRRENGLFGVPLAVLLENDQKKFPGIKVPLIFQKLLLKLEETGLETEGILRVPGSACRVKSLRQEIEAKFDEDTFDWDQVRNNDAAGLLKMFVRELPSPLFPVEYLPAFITLVEKIPKTKLQLQALHLLIMLLPESSRDAAKALLQFLKKVVANENKNKMTLWNVSMIVAPNLFICKGKHANQEEMLAAATTAHVVRLLIKYQDILWMVPTFLITQVRKMNEAAMNNMKKQLMFDKGVRKLLRRKTMDRERPERTESDIPEGVIRVHAPLHSKVSMAIQPNNQTKAKDIVARFYYENRPGSSEELKMPIQSLYEIGGNIGEHCLDPDAYMLDVYRANPQAEWVIKEKRSSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XED4</accession>
    <name>A0A6I9XED4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein-sorting-associated protein 25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ESCRT-II complex subunit VPS25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">VPS25</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909473.1">
        <property type="nucleotide sequence ID" value="XM_014053998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XED4"/>
    <dbReference type="GeneID" id="106539245"/>
    <dbReference type="KEGG" id="tsr:106539245"/>
    <dbReference type="CTD" id="84313"/>
    <dbReference type="OrthoDB" id="245150at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000141">
        <property type="entry name" value="vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.570:FF:000001">
        <property type="entry name" value="vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.570">
        <property type="entry name" value="Winged helix' DNA-binding domain. Chain C. Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008570">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014041">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05871">
        <property type="entry name" value="ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013909473.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="20790" checksum="BAE363CFA138DEFF" modified="2020-10-07" version="1">MMSFEWPWQYSFPPFFTLQPNVDTRQKQLTAWCSLVLSYCRLNKLYTMTVPEAQESPLFNNWKLQRKLSLESILVVLEELRKKGNLEWLDKNKSSFLILWRCPEEWGKLIYQWVLKNGSTNSVFTLYELSNGEDTEEEEFHGLEELVLLRALQALQQEHKAEIFTLNDGRGVKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFH5</accession>
    <name>A0A6I9XFH5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cadherin-like and PC-esterase domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106541891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912926.1">
        <property type="nucleotide sequence ID" value="XM_014057451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFH5"/>
    <dbReference type="GeneID" id="106541891"/>
    <dbReference type="KEGG" id="tsr:106541891"/>
    <dbReference type="OrthoDB" id="1932925at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025883">
        <property type="entry name" value="Cadherin-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057106">
        <property type="entry name" value="NXPE4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14776">
        <property type="entry name" value="CADHERIN-LIKE AND PC-ESTERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14776:SF1">
        <property type="entry name" value="CADHERIN-LIKE AND PC-ESTERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12733">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cadherin-like beta-sandwich-like" evidence="1">
        <location>
            <begin position="295"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="NXPE C-terminal" evidence="2">
        <location>
            <begin position="459"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912926.1"/>
        </source>
    </evidence>
    <sequence length="743" mass="85093" checksum="114F3BC156040FF7" modified="2020-10-07" version="1">LLQLQTFFQTFLRAASPQQALDDMKEAISKLLLITEVFSEAALPQPQTTRQCSSCFQMLTFDIGFGHTFYPIVLEVHEYFDFQADEDSFSEDQSVKEILLRDTFRILLSNQTSSSTFFEAMQNVYRSARIKDDHLKENSDYCLTLEELNPLINFVKGIQNPGEFELLFPSAVPKIHFLLHDLYRIVNPGKKLNSILALHWFLSSLFKQFQGISNIASANLPKGQNRSRNKSLMTAKWSNLGISYSEKDNIQLRVSSPVKEKDKELHILKLAKEMHCSHDKDTLPHIRQIITSPHLDLNPNFDPKIKEYYTEVPFDLLTIKIRAEPMNCQGEAHLDERQGPSSAHYPLGLGINIVIIVVTDKSQSVNEIVSIYKIIIFREDRPSLPLFDDFMLCGFIQDCGSVIHPEESCGLQPLSAEYLAIVSQPQLKTCEMGDVKGQWIVPCLSCSDNRTCDWRQITWQPRNCQYPVLNKPELQKCVERKKVLFIGDSTNRGMMYYLIERVNETLQEWQKAHGMKFYHNVNNGSTFISYSYYPQFWISVSQRSTFEEALEQLLSRSQPLKNTGQTILVIGGVQWLNFSHLHIIQKVLKRMNLSNIMVVIKSLGMGFHLPVDGVHTLSSVEIQNLWKENLAILNTAKHYGYEVVDTFIITMGRYKEFLQGKCGCHFHEVVKSKASEKSFPIATTFSRPYVLGKYFNNQSNVAKLQAYASNSQSPYHVRGPINQVYSEILLSRICGNNRKDASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH13</accession>
    <name>A0A6I9XH13_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rho GTPase-activating protein 23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARHGAP23</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914994.1">
        <property type="nucleotide sequence ID" value="XM_014059519.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543487"/>
    <dbReference type="KEGG" id="tsr:106543487"/>
    <dbReference type="CTD" id="57636"/>
    <dbReference type="OrthoDB" id="6281275at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06756">
        <property type="entry name" value="PDZ_ARHGAP21_23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01253">
        <property type="entry name" value="PH_ARHGAP21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04395">
        <property type="entry name" value="RhoGAP_ARHGAP21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000014">
        <property type="entry name" value="Rho GTPase activating protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000066">
        <property type="entry name" value="Rho GTPase activating protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175:SF5">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="135"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="916"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="1126"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="558"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="679"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1054"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="558"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="679"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="706"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013914994.1"/>
        </source>
    </evidence>
    <sequence length="1364" mass="152318" checksum="5B7DB19BFEBEB1CD" modified="2020-10-07" version="1">MVEEGRSRGDPAPCSGRQQRSSYFPASSQRGISVVSKAVWSVARTGNGWHFEQAVGVDCSLPEPRCIWLTSLRNNDAECWERRWRRAGAMMAWQKFQARHRRARKLTPKLVQRDGTATNANAPPMESFPWVGPKTVVLHKNLQGGYGFTLRHFIVYPPESAVHTNVKEEENGNRTVPPRNRLEPMDTIFVKNVKEDGPAHQAGLQTGDRLVKVNGESIIGKTYSQVISLIQNCEDALELSIMPKDEDILQLAYSQDAYLKGNEPYSGGAQSIPEPPPICYPRKNYPFQSRRDVSSAEIYQGQQVDNRQSYHSGSSGPSSPLNSTAVHKTHLSAWTESKQNFTSNHSSPAHCTEEVQYGMTQRAPIPRHIPTSGSFSQYPNSSCPGSVASSSPYIPSTYSMPNHGCYGPPKHLPDHHPHGGLKDAHREGRPSRECIRAGPKSISRLECQQALSNWISNQVPRRSSSEERCSAMPPRYRSVSQDHLGDVSSSRGWSHSASQETLIQSSAHDNWTYRARSDNYLMKYGQSMEALEQADLVSPSYGRCILPSDRFYQHGQINRAQPNHPNTCIPSASSRDPPSVHVQKHPSQPNLQSIDDSGYIGYRSYSPSFQRRTGLLHALSFRDATFGDLPTFNISQRQLSHSTPSYVEKSLPTAVLSSAPSTCPDPFLASSPEILMDQRSAKQENDVKSFEQISSSQAIEPLQSDAEERKDEVVLRQKPPTGRKMPPPARQMNFVFPSDLKETDICDPPATAPAANKENKRGVAPLATPEDSLAFIPFIDEPTSPSIDLKAKHIPASSVVSSAMNSAPVLSASPSSPTFTFAMNRHYSQDCSNIKASRRSSYLLAITTERSKSCDDGLNTFRDEGKLLRRLPSRVPSLRILRSFFTDGSLDSLGTSEDTRSKRHSTSDLSDVTFSDVRKEGWLHYKQILTKKGKKVGGGIRQWKRAFAVLRAHSLYLCKDRREAVALAPPPGEEEQPPISIRACLVDISYSDTKRKHVFRLTTADFCECLFQAEDRDDMLAWIKVIRESSKAEGEDPGFASQALINKKLNDYRKVSSSGTKPDSSPKGLRMSGMKSESQKQTGIAAPYSPRQDGVTVKDDSVPQKAPWGINLMKKSKKPVPRAFGIRLEDCQPAPDNKKIPLIVEACCKVVEDKGLEYMGIYRVPGNNAVVSSLQEQLNKGAAEINLQDERWQDLNVICSLLKSFFRKLPEPLFTDDKYNDFIEANRIEDASERMKTLRKLIRDLPGHYYETLKFLVEHLKTIADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHSDWFFSDKEEKGEKTPIDEQEAQSMPNIEYLLPNIGRTAASGDASADLPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHA4</accession>
    <name>A0A6I9XHA4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Testis-expressed sequence 33 protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TEX33</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913252.1">
        <property type="nucleotide sequence ID" value="XM_014057777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHA4"/>
    <dbReference type="GeneID" id="106542130"/>
    <dbReference type="KEGG" id="tsr:106542130"/>
    <dbReference type="CTD" id="339669"/>
    <dbReference type="OrthoDB" id="5977581at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029234">
        <property type="entry name" value="CIMIP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31702">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31702:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15400">
        <property type="entry name" value="TEX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913252.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="34992" checksum="84C6DE13811B61BB" modified="2020-10-07" version="1">MKLQLVPMDTAFPMPATPYHVNKVTYRPTTEKPGYKSKEQVTYNKAGVPSSKGLSSRTSSANSWESSKNLEGDKTRDEFLCPTPPSTQHFPAKSHNKHNHNPKTPAGQYIHHESPKICAERKGSFDSKISLENHPKGRAARSQEHAKSVQEKEESISFNSDDSTGKLHQSVRTELLSSPTKNIKCSYEDTEEVKTVLNAKDSLASGIQNVKVEGKCLQEKKNSRIPANIKAKYGTTVVEKLISEEQARRALCEAGLIQGQKRLSDWPFKSLENPMTSSPYADYYELGYNLRSNIFQGELDKSQCFLFWSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIM4</accession>
    <name>A0A6I9XIM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein phosphatase 1E</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Ca(2+)/calmodulin-dependent protein kinase phosphatase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">CaMKP-nucleus</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Partner of PIX 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Partner of PIX-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC106544008</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013915619.1">
        <property type="nucleotide sequence ID" value="XM_014060144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIM4"/>
    <dbReference type="GeneID" id="106544008"/>
    <dbReference type="KEGG" id="tsr:106544008"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000021">
        <property type="entry name" value="Protein phosphatase, Mg2+/Mn2+-dependent, 1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF535">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 23" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="25">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013915619.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="56913" checksum="075331785937EFC5" modified="2020-10-07" version="1">MRRKMEDKHVCIPDFNTLFNLEDQGEQAYFAVFDGHGGVDAAIYASIHLHVNLVHQEAFQHDPAEALCKAFQVTDERFVQKAARESLRCGTTGVVTFIRGNMLYVAWLGDSQVMLVKRGQAVELMKPHKPDREDEKKRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSGSTVLDGSEDYLILACDGFYDTVNPDEAVKVVADHLKENNGDSTMVAHKLVASARDAGSSDNITVIVVFLRDMNAIISAGEESEWTENSFQGGQEDSGDDKENQGDCKRQWPQHQVSAPADLGYDGRVDSFTDRTTLSAGSAINVFEDPSCLDLRKTEAGKLHSVKRLSPDQVFSAGVPKRAILSQGVLGDGKRMDDVPPHGQNALLGSPSPGECSVVLVEAPIPTGPEGLWFKSWGRKASIFSHFHFCDAKRWQRLARLKPKLNGLFFEQRSLFCPESLTLCSPNHLNGRRNKFLRSHLGQVSLGCHPGHGESIFLMLRPSPCIPPDPWLLWSCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJT9</accession>
    <name>A0A6I9XJT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CLIP-associating protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106540530</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911158.1">
        <property type="nucleotide sequence ID" value="XM_014055683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJT9"/>
    <dbReference type="GeneID" id="106540530"/>
    <dbReference type="KEGG" id="tsr:106540530"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000031">
        <property type="entry name" value="CLIP-associating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF30">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="7"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="168"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="234"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911158.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="50676" checksum="FAFE88105F880C27" modified="2020-10-07" version="1">MEHGKMDYFCEQVQQKDVGRRLQVGQEFLEYLNDPSISTDLEQDQQCLDKVIDELTRWVNSSNYKVSLLGLDLLGAFGERLLGRFRTYVGTVLVALMDRLGDAKDQVREQAQNLILKLMDQVAPPMFIWERLAAGFKHKNYRSREGVCLCLVATLNVYGAHSLILSKLVPHLCLLSGDSSSQVRDAAIVALMEIYKHVGEKVRVDLNKRGIPAARLQTIFMKFDEVKNSDSMISSSISDKSIDDEESGDGNRPSATASGFKVPAPKKPGNPSNTTKKPGSAGGPKVVGTTKEGGAGAVDEDDFIKAFTDVPTVQIYSSRELEETLNKIREILSDDKHDWDQRTNALKKVRSLLVAGAAQYDGFFQHLRLLDGAFKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRLKMASTQNLTKIGGGKLHLHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ23</accession>
    <name>A0A6I9XQ23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106545929</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918134.1">
        <property type="nucleotide sequence ID" value="XM_014062659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ23"/>
    <dbReference type="GeneID" id="106545929"/>
    <dbReference type="KEGG" id="tsr:106545929"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF12">
        <property type="entry name" value="PARVALBUMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918134.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="14350" checksum="32F6DBDA3260C21A" modified="2020-10-07" version="1">MAFAGILTDADIAAGLQSCKAADTFSCKTFFSKSGLHTKSRDQLAKVFGVLDQDKSGYIEEDELRVKSSKVSASNVYFTPTDMIGTAKSDAGFTQFTIICFSDRSELQGYRFLTSLHTYKVTEFVVLVTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ44</accession>
    <name>A0A6I9XQ44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-conjugating enzyme E2 K</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">E2 ubiquitin-conjugating enzyme K</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Huntingtin-interacting protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ubiquitin carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2-25 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ubiquitin-protein ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">UBE2K</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with RNF138/NARF. Interacts with BRCA1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013912823.1">
        <property type="nucleotide sequence ID" value="XM_014057348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ44"/>
    <dbReference type="GeneID" id="106541811"/>
    <dbReference type="KEGG" id="tsr:106541811"/>
    <dbReference type="CTD" id="3093"/>
    <dbReference type="OrthoDB" id="9993688at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14390">
        <property type="entry name" value="UBA_II_E2_UBE2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23800">
        <property type="entry name" value="UBCc_UBE2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000010">
        <property type="entry name" value="Putative ubiquitin-conjugating enzyme e2 k"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000021">
        <property type="entry name" value="Putative ubiquitin-conjugating enzyme e2 k"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042599">
        <property type="entry name" value="UBE2K_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="16">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="15">
        <location>
            <begin position="143"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013912823.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20645" checksum="FD79D37A1B04D45F" modified="2020-10-07" version="1">CKIFIYKIYFNLIFVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNSEMFKQTARLWAHVYAGAPLSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVERATELLLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQH6</accession>
    <name>A0A6I9XQH6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bcl-2-associated transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BCLAF1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCLAF1/THRAP3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916041.1">
        <property type="nucleotide sequence ID" value="XM_014060566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQH6"/>
    <dbReference type="GeneID" id="106544319"/>
    <dbReference type="KEGG" id="tsr:106544319"/>
    <dbReference type="CTD" id="9774"/>
    <dbReference type="OrthoDB" id="9948513at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029199">
        <property type="entry name" value="THRAP3_BCLAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15268:SF4">
        <property type="entry name" value="BCL-2-ASSOCIATED TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15268">
        <property type="entry name" value="THRAP3/BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15440">
        <property type="entry name" value="THRAP3_BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="637"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="861"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="637"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="750"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="762"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="808"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="896"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="906"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013916041.1"/>
        </source>
    </evidence>
    <sequence length="917" mass="105969" checksum="1F60F7FE1D078138" modified="2020-10-07" version="1">MGRSNTRSHSSRSKTRSHSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRVYNRDYRRDYRNNRGMRRPYGYRGRGRGYYPGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYKSSRSPRSSSSRSSSPYSKSPVSSKRRGSSEKQPKKTEAAALQNSPLKSKSQEEEKNTFEHDPSEALDDFNKSAAASADIWPGLSAYDNSPRSPHSPSIASPPSQSSSCSDAHLLSTAHSAKDTPQHSHSIQHSPERSGSGSLGNGSSRYSPSQNSPLHHIPSRRSPAKAVASQNAAREETRMRSFYPEAGEQESAKGGKFLKRYTDEESRVYLLDRNNTREKDAQKDRGSEKGRTEGEREWEDQDALEYYMDKEPGKQKFNYSEGEDTEETEDYRQFRKSVLADQGKNFAASHRNVEEEGSKYKSKISMKANREGEAFREDKGYKVKETTSYIVERPILVKDKHKEDEKVSERMMKKEIQSPEQVKSEKLKELFDYSPPLHKNLDMREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQASKSTSESFIQHIVSLVHHVKEQYFKSAGMTLSERFTAYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLSGEERTFKEETQKGDKKLKCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKVPKDYKDFKTFKDDSKQKREQDHSRSSSTSSPSSSSSSREEKDCKKERDEELKPHHEQKEYPGFTGVGRPRGTFFRIRGRGRARGVFAGTNTGPSNSNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGLVEDEEETMETEDKERRKEEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQQ3</accession>
    <name>A0A6I9XQQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TUBB4B</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913038.1">
        <property type="nucleotide sequence ID" value="XM_014057563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQQ3"/>
    <dbReference type="GeneID" id="106541969"/>
    <dbReference type="KEGG" id="tsr:106541969"/>
    <dbReference type="CTD" id="10383"/>
    <dbReference type="OrthoDB" id="9004456at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000006">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013913038.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="49803" checksum="A54DD52822AFA072" modified="2020-10-07" version="1">MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEAEEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQY2</accession>
    <name>A0A6I9XQY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Elongation factor Ts, mitochondrial</fullName>
            <shortName evidence="8">EF-Ts</shortName>
            <shortName evidence="8">EF-TsMt</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106544658</name>
        <name evidence="8" type="synonym">TSFM</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916477.1">
        <property type="nucleotide sequence ID" value="XM_014061002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQY2"/>
    <dbReference type="GeneID" id="106544658"/>
    <dbReference type="KEGG" id="tsr:106544658"/>
    <dbReference type="OrthoDB" id="277235at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14275">
        <property type="entry name" value="UBA_EF-Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000031">
        <property type="entry name" value="Elongation factor Ts, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.20:FF:000007">
        <property type="entry name" value="Elongation factor Ts, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.20:FF:000008">
        <property type="entry name" value="Elongation factor Ts, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="entry name" value="Elongation factor Ts, dimerisation domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="ELONGATION FACTOR TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741:SF0">
        <property type="entry name" value="ELONGATION FACTOR TS, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25025">
        <property type="entry name" value="EF-Ts_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="Elongation factor Ts (EF-Ts), dimerisation domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Translation elongation factor EFTs/EF1B dimerisation" evidence="9">
        <location>
            <begin position="218"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013916477.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="47168" checksum="02BC7F55C44DBD10" modified="2020-10-07" version="1">MAALVFAGDVEVGLESVFPQELELFADAFPVQTRYRLCGHELSITQYHGARLGVAAPVWEAALALCEYFEAKKVNFQDKKVIELGAGTGVVGILASLLGGHVTITDLPVALKQIEENVHRNLPAQCIARTKQWHLPRFLHLGIPALAAKEALMKLRKKTGYSFVNCKKALEKYVDNIEEAEVWLHEQAKKEGWSKALKLQGRKTKEGLIGLLQEGSSAVMVEVNCETDFVAQNVKFQQLVQQVAIGTMIHHKEIIDQSNTYAKRFLNSDELSQLRTGPEGSLLTDQLALAIGKLGENMVVKRAACISVPENFFIGSYVHGSPSESSSWLSNMLFGKYGAMVICSSSEEDPKSDITELSRKLAQHVVGMAPLSVGSAEDEPGDESETKMLAQPYVMEPTISLGQFLQPTGISVLDFVRFECGEDPELSESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRU5</accession>
    <name>A0A6I9XRU5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Clarin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CLRN1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the clarin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913423.1">
        <property type="nucleotide sequence ID" value="XM_014057948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRU5"/>
    <dbReference type="GeneID" id="106542274"/>
    <dbReference type="KEGG" id="tsr:106542274"/>
    <dbReference type="CTD" id="7401"/>
    <dbReference type="OrthoDB" id="10012538at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050957">
        <property type="term" value="P:equilibrioception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026748">
        <property type="entry name" value="Clarin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548">
        <property type="entry name" value="CLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548:SF4">
        <property type="entry name" value="CLARIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25807">
        <property type="entry name" value="Clarin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913423.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="25852" checksum="C4B48BE757523786" modified="2020-10-07" version="1">MPAQQKKVLFCIAGVFSFACALAIAAAIGTQLWIRGKIICKTGALLVNATGQELDKFIGEIQYGLFYGEKVRQCGLGGRPFQFSFFPELFKIMPASIHVSVILFCLALFVFALIATGFFMYNAFGTPYETLHGPLGLYLWSFVSVTCGFLILILFSSEVKIHHLSEKIANYKEGSFIFKTHSEQFAKSFWIILVCSLVHFMNILLIRLAGFAFPFSKSKNSEAPNGAMDIMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRU8</accession>
    <name>A0A6I9XRU8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106537761</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106537761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013907465.1">
        <property type="nucleotide sequence ID" value="XM_014051990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRU8"/>
    <dbReference type="GeneID" id="106537761"/>
    <dbReference type="KEGG" id="tsr:106537761"/>
    <dbReference type="OrthoDB" id="10029135at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907465.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="23320" checksum="387B6A9A0EDB0FB1" modified="2020-10-07" version="1">MYDEYGEVVVESDGGYYYSPQGTSTEAEWAKKKRIKLVLDPEYESSSTGEDSAPESQRNRLSNPNIPTNGNSNIYIAQNGSVIRTRRSCLSNNLKVASPARLGKQFKKLDKLGVTHEENIPLNTFTAGAKSNDKLNPRPCSVSFAASSALDPDSIAIKAAGSRLKSTDQQESIVDNEGVKETLDLPSDHAQSEDEELWMGPWNNLHIPMTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS56</accession>
    <name>A0A6I9XS56_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine zipper putative tumor suppressor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106544983</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916892.1">
        <property type="nucleotide sequence ID" value="XM_014061417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XS56"/>
    <dbReference type="GeneID" id="106544983"/>
    <dbReference type="KEGG" id="tsr:106544983"/>
    <dbReference type="CTD" id="9762"/>
    <dbReference type="OrthoDB" id="10030037at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061001">
        <property type="term" value="P:regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045329">
        <property type="entry name" value="LZTS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2 HOMOLOG-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354:SF6">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06818">
        <property type="entry name" value="Fez1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="52"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="504"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="172"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="356"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="424"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013916892.1"/>
        </source>
    </evidence>
    <sequence length="530" mass="61247" checksum="47BDAE44683FA21B" modified="2020-10-07" version="1">MKLFYWSSFFPFQNTEPLVRPSAFKPVVPKNFHSMQNLCPVQSNGVTTENRKTLNHTHSNSPAPVKSGLEKASLNRTTNPERVGLSDSGRNSLTSLPTYGTGYSQHVAPMSASTSHINRIGTTYGEKNVVGYNGISTSDSGRSSTKSTSSFSRLNHLNETMPFHSPSTDDIIQDLENRLWEKEQEVLQMRRNLDKSEAAIYQVFEEKQKIWEREMDELRQNYANKLQQVSKKAQRAQQALQLQIFKLQQEKKKLQDDVAQLLQQREELEKKFVAFKKEQAEFLPKIEETKWEVCQKAGEISLLKQQLKDSQADVSQKLNEIVGLRTQLKEGKGFLRDKDEQILTLKDSYSTKSVSLDFCENELQLKMAEVQMLREKLGQCELEVSNLKQTLAHLGGPQNHALPELPEKLARDPLACESDEAKVKRQSEENLNALRREVERLQAELKAERQFREQQVLDFEEERCTWQEEKEKVIKYQKQLQLNYVEMYQKNQLLEHKVNEMANKATGLSHPDEKKTWTPSRLERIESTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT57</accession>
    <name>A0A6I9XT57_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kelch-like protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106542600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913853.1">
        <property type="nucleotide sequence ID" value="XM_014058378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT57"/>
    <dbReference type="GeneID" id="106542600"/>
    <dbReference type="KEGG" id="tsr:106542600"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF15">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913853.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="25052" checksum="A35B7A25CA9D8710" modified="2020-10-07" version="1">MLVPRTRFYAGVLNERLVAVGGGAALGMPIDTAEEYRLAKNAWWPLPPFPVPVADHAGATHGGILYISGGFAEGQTLSTMYSYLSRLRCWVRNRPMAFARCDHGMAAVGDRIFCLGGRALAQGQEWVPVSEAEYYCPNTDQWAQLRLPAVDLCHFGLTTSQLQLYVTGGGSLRRRSKEEGVWVCHPSRGTWLKVGCLPRALADHACCFVHLPPGIASPGEAERGGPLDGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTD9</accession>
    <name>A0A6I9XTD9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Draxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DRAXIN</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919324.1">
        <property type="nucleotide sequence ID" value="XM_014063849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTD9"/>
    <dbReference type="GeneID" id="106546871"/>
    <dbReference type="KEGG" id="tsr:106546871"/>
    <dbReference type="CTD" id="374946"/>
    <dbReference type="OrthoDB" id="9931375at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029094">
        <property type="entry name" value="Draxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28610">
        <property type="entry name" value="DRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28610:SF1">
        <property type="entry name" value="DRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15550">
        <property type="entry name" value="Draxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026833668" evidence="6">
        <location>
            <begin position="24"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="108"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="242"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013919324.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="35365" checksum="4B09A81A3AF8252E" modified="2020-10-07" version="1">MAHPSLWLFSVLFLFLMITGGLSSRPRMAANVPENDNSFPNRDSWMRVTPYQHRQGMGNRQTDRDEMPPGSPGSRETGGPSLGGTESRGLDRPANLRQKGAFVGFAFPYPEQENHNNPVPERAKKQGRAHRRHTRRKRLKQHRGKGSKLRPRALVKKPDSHVEHFRPFQGEGAAVGSAPTSLPSPVAQTLIEDPLPPVRAISSQPQLREWQAGDVMPTLDMALFDWTDYEDLRLDMWTSDRKKGRTHKSNNETTSAEGEPCDHHLDCLSDPGSEEEEEEKLEPGLSERDGTSPVECLERPGASSGIHLLPYWLQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTR8</accession>
    <name>A0A6I9XTR8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase HUWE1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106545179</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917131.1">
        <property type="nucleotide sequence ID" value="XM_014061656.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106545179"/>
    <dbReference type="KEGG" id="tsr:106545179"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000007">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="11">
        <location>
            <begin position="1445"/>
            <end position="1781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="255"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="531"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="834"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="882"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1205"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1301"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="255"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="531"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="563"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="632"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="690"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="836"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1222"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1240"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1301"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1323"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="1748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013917131.1"/>
        </source>
    </evidence>
    <sequence length="1781" mass="198204" checksum="217BCCABD6CD15AC" modified="2020-10-07" version="1">MVYLDDILIYMETCKEHVKLVRSVLKKLRAVELYAKLCKCEFHQEKIDYLGYHISHDGIKMDPAKQDNWVEFLPHAEVAYNNSVHSSTGFTPFKIVFGHDFVPIPEVPREKPQVLSLSEWSDQLRKIWPLAYRVLDAAHRAPKNQTDKKRTKPKEYKIGDRVFLSTKFLQTTQKSKKLGPKYVGPFPIVENELEDLIDELLEQDGGSGNSTIIVGRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEEDEDDQDDEEGEEGEEEEDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPATHPLMVRHADHSSLTLGSGSSTTRLAQGIGRSQRTLRQLTANTGHTIHVHYPGTRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDEFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIINHLEALRDEELEERREKRRKQLAEEEAKVSDKAKDSEDKEQNPQGPVPQSNSSAEQNLPDGATHPDGYPAVSAAAKDTSAGSKQTLVTLESPQQQQQQQATGAGELPSELVGQSTAQASGQLLMPVEQEDSGPGRLSTDAEATQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCNLEESVAESGGASTVEQAGTDAVVAAADASQQGPNAHTAREGQPDPSQQVDDGSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGINLPEGVDPSFLAALPDDIRREVLQNQLGIRPPARSAPTTTATTPTPPVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQSTSSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHSRPSGSNVDSLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRNWVIRSLLSILQRSSESELCVETPKGTVAPDDKGKRAAKGCGADSRPLDLLHKMESKSSGQLSWLSVSMDAALGCRTNIFQIQRAGGRKHTEKHAAAGSTVHIHPQAAPVVCRHVLDTLIQLAKVKTLVCFSSLQSESNQAEGSVRRDESPMDVDQPSPTTQDTQSVVLEGAQQGEKEKEERPPDLPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQANSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESYEKLRHMLLLAIQECSEGFGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWL7</accession>
    <name>A0A6I9XWL7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">JmjC domain-containing protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">JMJD8</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920569.1">
        <property type="nucleotide sequence ID" value="XM_014065094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWL7"/>
    <dbReference type="GeneID" id="106547826"/>
    <dbReference type="KEGG" id="tsr:106547826"/>
    <dbReference type="CTD" id="339123"/>
    <dbReference type="OrthoDB" id="438164at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF21">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="70"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920569.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="23378" checksum="E06CDBB422767C58" modified="2020-10-07" version="1">KLEVSLHPDSLLCPQEFQAQCTKDQLLARFGEHLVRLSTANTYSYRKVDLPFQDYVAHLLEPQDLASLGSETLYFFGDNNFTEWGSLFKKYNPPPFRIPGTTEAYSFGIAGSGSGVPFHWHGPGFSEVIFGRKHWFLYPPEKTPAFHPNRTTLSWFLDTYPSLAPWDKPVECTIHPGEILYFPDRWWHATLNLDTSVFISTFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY31</accession>
    <name>A0A6I9XY31_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Semaphorin-6D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SEMA6D</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915513.1">
        <property type="nucleotide sequence ID" value="XM_014060038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY31"/>
    <dbReference type="GeneID" id="106543933"/>
    <dbReference type="KEGG" id="tsr:106543933"/>
    <dbReference type="CTD" id="80031"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000004">
        <property type="entry name" value="semaphorin-6D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF65">
        <property type="entry name" value="SEMAPHORIN-6D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="641"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="8">
        <location>
            <begin position="66"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="768"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="828"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="901"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="768"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="915"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="964"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013915513.1"/>
        </source>
    </evidence>
    <sequence length="1057" mass="117741" checksum="CF759761E76ADDC8" modified="2020-10-07" version="1">MENQPQVNADFLPLLQVNRLASCAPSPTPPRPHPFHTAAMTFHPLAAYALLLAISQCWAVSFPEDDEPINVVDYHYSKQYPVFRGRPSGNESQHKLDFQLMLKIRDTLYIAGRDQVYTVNLNDIPKGEVVPSKKLTWRSKQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNAFNPMCRYYRVRTNAQTRPDCSLPKFKEHRDALQRFLINLTIVLLILFCLWRDRSISAHHSFHGQCLRSQNNPANVMGMVKRTKKLCLCNWTKSTMRCSLRSPAASLEFPSVVVSVMDRVKREKHPLLPRIELSAFQVEGKHFHQYTTLSTSHSAPPGSCAKHSPAEAYKTSIDFPDETLSFIKSHPLMDSAIPSVIEEPWFTKTRVRYRLTTIAVDHSAGPYQNYTVIFVGSEAGMVLKILAKTKAFSLNDSILLEEIDAFNHAKCNGDGEEDKKVVSLQLDKEHHALFIAFSSCIVRIPLSRCERHGSCKKTCIASRDPYCGWLAHGSCGRVRASMLLSLFVSYNHSIGGYEQDVEYGNTAHLGDCHEILPTTTTPDYKIFGDPTSDMMLSSASITTVGSIPVVSPKVIGSWKPKVTGSRKFVARDDPNASDYSEPLSGVPKGVRWEVQSGDSNQMVHMNVLITCVFAAFLLGAFIAGVAVYCYRDVFVRKSRKIHKDAESAQSCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTGRKELPQTADTKSMLMVDHRAQPPELAALPTPESTPVLQQKTLQGMKSQMEKAQNNLSSSRKEAPLKSPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNMSFSNSNAHKVDKKMPNVDHPLTKSSGKRDHRRSVDSRNTLNDFLKHLNEAPSNSKAIMADLQVAHQTLMLDSMGNVTEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPNAPAAPMTSLERQRGYHKNSSQRHSISALPKNLNSPNGMLLSRQPSVNRGGYMTPTGGTKMDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGIKRMPSLKPDVPPKPSFAPQTPSVRPLNKYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY63</accession>
    <name>A0A6I9XY63_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="36" type="primary">TRPM7</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="31">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="31">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013918731.1">
        <property type="nucleotide sequence ID" value="XM_014063256.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546390"/>
    <dbReference type="KEGG" id="tsr:106546390"/>
    <dbReference type="CTD" id="54822"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055080">
        <property type="term" value="P:monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16971">
        <property type="entry name" value="Alpha_kinase_ChaK1_TRMP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1010:FF:000002">
        <property type="entry name" value="Transient receptor potential cation channel subfamily M member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.200.10:FF:000001">
        <property type="entry name" value="Transient receptor potential cation channel, subfamily M, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000129">
        <property type="entry name" value="Transient receptor potential cation channel, subfamily M, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1010">
        <property type="entry name" value="TRPM, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
        <property type="entry name" value="TRPM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029601">
        <property type="entry name" value="TRPM7_a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041491">
        <property type="entry name" value="TRPM_SLOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032415">
        <property type="entry name" value="TRPM_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037162">
        <property type="entry name" value="TRPM_tetra_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF8">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY M MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18139">
        <property type="entry name" value="LSDAT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25508">
        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16519">
        <property type="entry name" value="TRPM_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17 30" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="8" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="20 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 31" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 30" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="36" id="KW-0675">Receptor</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 33" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="15 31" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="856"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="923"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="957"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="988"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="1081"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="1580"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="34">
        <location>
            <begin position="1767"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="543"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="2005"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="32">
        <location>
            <begin position="543"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="32">
        <location>
            <begin position="560"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="32">
        <location>
            <begin position="2020"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="29">
        <location>
            <position position="1940"/>
        </location>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <position position="1797"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <position position="1821"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <position position="1893"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="1926"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <position position="1942"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <position position="1950"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <begin position="1967"/>
            <end position="1973"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="1983"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="1985"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="1989"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00025760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR629601-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR629601-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PIRSR" id="PIRSR629601-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="RefSeq" id="XP_013918731.1"/>
        </source>
    </evidence>
    <sequence length="2038" mass="231384" checksum="4E1364D5865B7975" modified="2020-10-07" version="1">MSQKSWIENTFTKRECVYIIPSSKDPHRCLPGCQICQQLVRCCCGRLVRQHACFTASLAMKYSDVKLGENFNQELEEWSVEKHTEQCSTDAYGVINFQGGSHSYRAKYVRLSYDTRSEAILQLMLKEWQLELPKLVVSVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWIITGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGKDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVKLRRELEKTISQQRIHARIGQGVPVVALIFEGGPNVVLTVLEYLQESPPVPVVVCEGTGRAADILAYVYKQTEEGGSIPDGAEPEIISTIKKTFNFGQSEAVHLFQTLLECMKKRELITVFHIGSDEHRDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPALFHLVRDVKQGNLPPGYKLTLIDVGLVIEYLMGGTYRCTYTRKRFRVIYNSLSGSNRRSGRNASGSTPQLRKSHEPFGNRVDKKEKMRHNHFIKTAQPYKPKADTATDEGKKKKTKDDIVDIDDPETRRFPYPLNELLLWAVLMKRQKMALFFWQHGEESMAKALVACKLYRSMAYESKQNDLVDDTSEELKEYSSEFGELAVELLEQSFRQDETMAMKLLTYELKNWSNATCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILLPPTILMLEYKTKAEMSHIPQSQDAHQMTMEDSENNFQNIAEEIPMEVFKEVQVLDHSDVKHDMESMIKPKRLPITRKFYAFYHAPIVKFWFNTLAYIGFLMLYTFVVLVKMEELPSVQEWIVIAYIFTLAVEKIREIFMSEAGKISQKVKVWFNDYFNISDTIAIITFFIGFVLRFGAKGNFHENTYKENYVFVAGRLIYCLNIIFWYVRLLDFLAVNQHAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPNEEPSWVLAKDIVFQPYWMIFGEVYAYEIDVCANNSEAKHRCVTGSWLTPFLQSVYLFVQYIIMVNLLIAFFNNVYLQVKAISNLVWKYQRYHFVMTYHEKPILPPPLIILSHMASLCYCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEELCVEMYFSEKDDKFHCRSEERIRVTSERVEQMCIQIKEVGDRVNYIKRSLQSLDTQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLTQNLIDDVPLRSSMWRKHSIGNAFGSSFPQGGLDANNAVLCNISMQEEKDPGHKFSGFTKGDEFNFPEASSSGGALLPKALSPTELRQRVQGIQVSKYSNKVKKGGSSLNSLPYLTSLSAKFFVSTPSQPICKNQPGSTSKEEDVVVPKAAEGEHAVEFGAFVGKYHQLKRGHPAIAGGCCKVPSSGFFWLASISQNGGGYVKSGFIEEENDGAAAENLSDQENGQTASSFAKTVARSKFQVTLAKETRSVPCSGLKKTREVESRNRSFPEMSAKISSLSSGVTVQHQKLWIKSKLQGLIYQELCVMPLWILWYFWCALVFNVLISSSSPNGSLLCESVNSLEQQEDFKKAILEGMEKTKEKVSSNAEAPLRHVSSCEGFSDYYRAARSEQTHNKSRRASTEENHQVDSKAALLTDWLQGRTSYRNEMSSEEDALNGIASPFKPIMDTTYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRALKVACTWSEYDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNMLEEVMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSSDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKILLPQEDSPELTIQPGSCTKESNSTGSRHLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ12</accession>
    <name>A0A6I9XZ12_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bicaudal D-related protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106547044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919547.1">
        <property type="nucleotide sequence ID" value="XM_014064072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ12"/>
    <dbReference type="GeneID" id="106547044"/>
    <dbReference type="KEGG" id="tsr:106547044"/>
    <dbReference type="OrthoDB" id="9451547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055107">
        <property type="term" value="P:Golgi to secretory granule transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF12">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919547.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="25667" checksum="7ECAA418AA430B95" modified="2020-10-07" version="1">MSASCLPAALSAPSELDSACRMENGPRTAGAAQPPPTAGKDAVLGSVALPAPLLPPAAAAAPSSSSPPPSAAAAPSPSVVGGELALALEEELAMLASAGEGSAESEPPGGPAAASPPAEPEVLLSQMRQKEKDLVLAARLGKALLERNQDMSRQYERMYKELTDKLEHLEQEKHELRRRFENKEGEWEGRVSELESDVKQLQDELEKQQAHLRDADREKTRAVQELSEQNQRLLDQLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ63</accession>
    <name>A0A6I9XZ63_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Collagen alpha-1(VII) chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106546747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919176.1">
        <property type="nucleotide sequence ID" value="XM_014063701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ63"/>
    <dbReference type="GeneID" id="106546747"/>
    <dbReference type="KEGG" id="tsr:106546747"/>
    <dbReference type="OrthoDB" id="196393at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005594">
        <property type="term" value="C:collagen type IX trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF918">
        <property type="entry name" value="COLLAGEN ALPHA-1(IX) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="654"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="692"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919176.1"/>
        </source>
    </evidence>
    <sequence length="804" mass="79466" checksum="933BCE67042DF6A0" modified="2020-10-07" version="1">MQPDKTIDVTLRYSLIITSSALESKSEDQAHMAVKVTGPPGLSVPGPPGPPGEQGENGLTGLTGKIGPKGDPGEPGERGESGRSGPAGPTGPRGKEGERGDKGEEGISGEPGLPGKPGDKGPRGPPGIRGLPGEKGDQGDPGEDGRNGSPGPSGSKGDRGSPGEPGPPGQVVGGGLGEAGQKGEKGEPGDPGEDGMKGTKGEVGLPGLPGERGIEGLRGVPGVRGDPGDRGPVGEKGDRGPPGLDGRNGLDGKPGQIGPLGQRGDPGKQGDPGRDGLQGQRGEQGPPGAIGPPGSPGLPGKTGEDGKPGLNGKNGEDGIPGEDGRKGEKGESGTPGKEGPEGVKGERGTSGPPGPPGPSGVPGVPGQVGQPGQGLPGLPGLSGPKGDRGEPGFKGEQGRNGETGPRGEPGTASNVERALEANGIKISLLREITGAYDGSSDLLPLFPDRRRGPKGERGDPGERGPPGKELGKALHTYTNKILNSVYIIFSWILYPKPKYHDDDVDKLVQHRGFPGERGAKGDKGNEGPQGPPGRAIGEQGPRGTPGLSGEPGKPGIPGLPGRAGELGEAGRPGEKGDRGEQGAKGEQVDSTVEGSVSGFPGERGPIGPKGSKGDPGEDGVKGSRGDKGEDGQKGERGDAGEKGRDGTPGPIGPPGTQGIPGIKGDVGEPGSSTRGMPGPPGTVGFPGPPGSSGPVGEPGKPGVPGRDGVSGKEGEPGPPGKMGVPGPSGPAGPKGEPGDAGSPGQSIVGPPGVKGERGEPGVFDEEFLKKPGLKGDRGLPGPRGEKVRKPFSRQEPKFSFSSIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1R2</accession>
    <name>A0A6I9Y1R2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="35">Serine/threonine-protein kinase TOR</fullName>
            <ecNumber evidence="35">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="41" type="primary">MTOR</name>
    </gene>
    <organism evidence="40 41">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:10597"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Lysosome membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Microsome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Mitochondrion outer membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 35">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="35"/>
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        <property type="nucleotide sequence ID" value="XM_014065412.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2250022at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016242">
        <property type="term" value="P:negative regulation of macroautophagy"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05169">
        <property type="entry name" value="PIKKc_TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000074">
        <property type="entry name" value="DJ576K7.1 (FK506 binding protein 12-rapamycin associated protein 1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000058">
        <property type="entry name" value="MTOR isoform 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase mTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000094">
        <property type="entry name" value="Serine/threonine-protein kinase mTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000140">
        <property type="entry name" value="Serine/threonine-protein kinase mTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.150:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000083">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.150">
        <property type="entry name" value="FKBP12-rapamycin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009076">
        <property type="entry name" value="FRB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036738">
        <property type="entry name" value="FRB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024585">
        <property type="entry name" value="mTOR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
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        <property type="entry name" value="TOR_cat"/>
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    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08771">
        <property type="entry name" value="FRB_dom"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23593">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11865">
        <property type="entry name" value="mTOR_dom"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01345">
        <property type="entry name" value="Rapamycin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47212">
        <property type="entry name" value="FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="25" id="KW-0007">Acetylation</keyword>
    <keyword evidence="22 35" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="31" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="26" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18 35 41" id="KW-0418">Kinase</keyword>
    <keyword evidence="29" id="KW-0458">Lysosome</keyword>
    <keyword evidence="28" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-0492">Microsome</keyword>
    <keyword evidence="27" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17 35" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="30" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="40" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 35" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="20" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="15 35" id="KW-0808">Transferase</keyword>
    <keyword evidence="23" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FAT" evidence="38">
        <location>
            <begin position="1401"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="37">
        <location>
            <begin position="2161"/>
            <end position="2474"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="39">
        <location>
            <begin position="2522"/>
            <end position="2554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="36">
        <location>
            <begin position="1834"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="36">
        <location>
            <begin position="1846"/>
            <end position="1865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00051942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="RuleBase" id="RU364109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="RefSeq" id="XP_013920887.1"/>
        </source>
    </evidence>
    <sequence length="2554" mass="290487" checksum="70E57E801B4824B9" modified="2020-10-07" version="1">MFLFSLLPRLLPDKIWLRLLLPPTENPGRLHATMSGTVIILQQFAGGLKSRNEETRAKAAKDLQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVSALRACLILTTQREFKEMQKPQWYRQTYEEAEKGFDESVAKEKGVNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLLGFGTKPRHVTPFSSFQSVQPQQCNALIGLLGYSPHPGILGFGTLPVPAKSTLVESRCCRELMEEKFDQVCYWVLKCRSSKNTLIQMTVLSLLPRLAAFRPSAFTADHYLPDTMSHVLSCVKKEKERTAAFQALGLLSVAVRSEFQVYLPKVLEIIKGALPLKDFTHKRQKSVQVDATVFTCISMLARAMGPSIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKEIQDGLLKMLSLVLMHRPLRHPGMPKGLSHQLASPSLTNIPEANDVASITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEVRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGVGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALIVKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVRSHIRPYMDEIVTLMRDFWVVNNSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDSSSTRSVSTKLLNAIQLFGANLDDYLHLLLPPIVKLFDAPDAPLSARKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRTTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEDDPLIYQHRMHRSSQGEALVSGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLGDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAASGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCQMLSELEEVIQYKLVPERREILRQIWWERLQGCQRIVEDWQRILMVRSLVVNPHEDMRVWLKYASLCGKSGRLALAQKTLVLLLGVDPSRQLDHPLPTLHPQVTYAYMKHMWKSARKIEAFQHMQHFVQMMQQQAQHAIATEDQQHRQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSASTEHDRNWYKAWHAWAVMNFEAVLHYKLQNQARDEKKKLRHASGTSITSANTEGSNSESEAESAENSPAPSPVQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPEVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIYQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQQIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEMLDSVELGETAHKKAGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2X8</accession>
    <name>A0A6I9Y2X8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Transmembrane protein 200C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12 13 14 15 16 17 18 19 20" type="primary">TMEM200C</name>
    </gene>
    <organism evidence="8 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM200 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917471.1">
        <property type="nucleotide sequence ID" value="XM_014061996.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917479.1">
        <property type="nucleotide sequence ID" value="XM_014062004.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917486.1">
        <property type="nucleotide sequence ID" value="XM_014062011.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917494.1">
        <property type="nucleotide sequence ID" value="XM_014062019.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917504.1">
        <property type="nucleotide sequence ID" value="XM_014062029.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917513.1">
        <property type="nucleotide sequence ID" value="XM_014062038.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917521.1">
        <property type="nucleotide sequence ID" value="XM_014062046.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917530.1">
        <property type="nucleotide sequence ID" value="XM_014062055.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917537.1">
        <property type="nucleotide sequence ID" value="XM_014062062.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917546.1">
        <property type="nucleotide sequence ID" value="XM_014062071.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917556.1">
        <property type="nucleotide sequence ID" value="XM_014062081.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917564.1">
        <property type="nucleotide sequence ID" value="XM_014062089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2X8"/>
    <dbReference type="GeneID" id="106545440"/>
    <dbReference type="KEGG" id="tsr:106545440"/>
    <dbReference type="CTD" id="645369"/>
    <dbReference type="OrthoDB" id="9994280at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018787">
        <property type="entry name" value="DUF2371_TMEM200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31815">
        <property type="entry name" value="AGAP005329-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31815:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 200C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10177">
        <property type="entry name" value="DUF2371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="402"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="442"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="258"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="482"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013917479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013917486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013917494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013917504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013917513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013917521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013917530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013917537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013917546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013917556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013917564.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="60737" checksum="E41711CD28204DBF" modified="2020-10-07" version="1">MIATGGLLRISARKQDPLRPQGQVSKHKRKAKKKLKNDVVVVKGKLKICSLSGLIAVCGILVLLVGIALAVVGYWPKPLYRTGGLGRSPLLKSYGNVPESHTKNYTQETRESYLEVTNSSFSGNQKALSPLPAPSSPSSSKNLLFHFFSRYLHSDKLKVLGPLIMGIGIFLFICANAVLHENRDKKTKVINLRDLYSTVIDAHSLRPKDGAPSAVASVPINGFVNCVQSRGLDLKPGSSSGGETLGATATLTKNTWHPSVSAPLSPPDLASSPRCFPTSRQEQIPSLADAVYSIYKERAALAQSVRNPSCTPTDSWDQHSTASSIVGSSLSTFTLLPLAQGETADRDYGDWQRLLGERDGQEIPRGEFELSLKDLRSSYLDTAQELTVLPRMNKRKVVLRRQSTSCLPDSRRSLFPEPSQTLGSSTDLDSSLLAKASSSSYSRSLDLGDSHLPTPSVDQKGSQSTQCDQYRSNKGYTPLEESGTSLESVANTPAHKAPEDCEVSSSEEIGSSQSTSKEQTEQQPLKIQRQYTNKEKLFMISRSDAIVGLEDADLENIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3J2</accession>
    <name>A0A6I9Y3J2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Interleukin-1 receptor accessory protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106540858</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911605.1">
        <property type="nucleotide sequence ID" value="XM_014056130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3J2"/>
    <dbReference type="GeneID" id="106540858"/>
    <dbReference type="KEGG" id="tsr:106540858"/>
    <dbReference type="OrthoDB" id="9166379at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000188">
        <property type="entry name" value="Interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF20">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="101"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013911605.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29871" checksum="F2F1C0290A576A8F" modified="2020-10-07" version="1">MYHDRVKQNLTCPDVQSFMPAGLEPQVRWYKECHLMKYDYPDRNPNGLNLTFAIVLSAYEGQYTCMASYRENGRDFSLTRTMRVKVVEAPERATAPLIIYPTKRIVYDRKPGEHLSLRCEVSFTFLKDSPQEVWWAIDGKHSDDVTDFKFNITTTEAPLPIGGTQIQKILSVPRVTEKDLKRNFTCFAQNNRGIVQAQAVVRIKPPVLRYTVELACGLGATVLLVVILTVIYHFYWLEMVLFYRAHFGTDETVLDGKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3L8</accession>
    <name>A0A6I9Y3L8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein LOC106545860</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC106545860</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918017.1">
        <property type="nucleotide sequence ID" value="XM_014062542.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918018.1">
        <property type="nucleotide sequence ID" value="XM_014062543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3L8"/>
    <dbReference type="GeneID" id="106545860"/>
    <dbReference type="KEGG" id="tsr:106545860"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="58"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013918018.1"/>
        </source>
    </evidence>
    <sequence length="649" mass="71944" checksum="0EC5574ACBC40324" modified="2020-10-07" version="1">MFLRSKVNFVERVQRPLPKEAMQDSYQNVMALGRTPLLPNYPAVAKDTQESQRQRAKFWSKEEIGLFVDLWISPEVQNELQNTYHNEVVFNWLSNEMGLRGFNRSARQCREKMNALKKKFKEVTTHNKCPGAGEVRTMPFYDKLATILLSRRDAKHSSNSGDKGQWSRQASSPAPGTSGQASLLARQSLISPPGLRSSGTASQSKSHLQQPPHKQPRLLQAGTVSPTHCHRPLLQPNRSLAMQKHRGHSSPWPTANQLAVVTPKIEEQSPEGENPPNSMCSVLQGSHLPMEVRVIDENGITVTSSMCEDVKQEQDEDDTAHDPELEFDGKFEREIVDDSDPPEATGTRLDPDVLSDYSEEPYDETEGVDDLPVDLLQCFGDRFSSFGGVEESNHEQERPKHEHPLSRALSTAESLIQEQEGEVLENSDIICAPLGNLGRKDLNASFEGVVGPPIDRVPPARGLGDDAPLTSAQRTARFRNKRKIERVQLAKELINATRETGENIREALYELDSKESQRRVDDRKVAVWCAKHIAKSIRVSSQTMAAAMRDSDAAFQRCMESYTVALERQTTLLENIADSLNARVSAPQPSLPTSTPADRTRDTSVCASPVPAPSPPIPMESEKTPPSLRSQPGNEEVSPGGADSNSSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y564</accession>
    <name>A0A6I9Y564_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membralin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMEM259</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912870.1">
        <property type="nucleotide sequence ID" value="XM_014057395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y564"/>
    <dbReference type="GeneID" id="106541854"/>
    <dbReference type="KEGG" id="tsr:106541854"/>
    <dbReference type="CTD" id="91304"/>
    <dbReference type="OrthoDB" id="6779347at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019144">
        <property type="entry name" value="Membralin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF4">
        <property type="entry name" value="MEMBRALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09746">
        <property type="entry name" value="Membralin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="616"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912870.1"/>
        </source>
    </evidence>
    <sequence length="692" mass="77605" checksum="73A1B4230C281E58" modified="2020-10-07" version="1">MSENQPNNTHHPLNNNGVGGAPAGNNRGVRNLNQNPLINVRDRLFHALFFKMAVTYARLFPPSFRRIFEFFILLKALFVLFILAYIHISFSRSPINCLEHVRDKWPRDGILRVEIQRNSSRNPVFLQFCESERFPGMVIEPAVGEEEEEEEEEEEMTVEMFQNSSIKFNLDIEPKIFHKATRLSNAHVLSPNESQEFSFGSEPSSKGMQPLSETVSEFEMLAKAVWPQEEYIVEYSFEYGFLRLSQSTRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLDEFLGYDDILMSSVKALAENEENKGFLRNVVSGEHYRFISMWMARTSYVAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMTIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQNQQVGTQTAVQDNLNNNTTVAAANSADQQPHLDASPAGAGDGSPASGEGHRPPTSSATVSLGNDLNWIADTAAVITEASFLSNLGSSFLDSPAVVTSGGSLQDPEPALNTAAQSLFSNDRTEAGSVTLEGTSTVGTSVTNTPPPVTVHPELEDIPQPSESSAGRAEPLKTDSPQGSALRGLPSQASEGLAERDPTPLSPENMIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5K5</accession>
    <name>A0A6I9Y5K5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Novel acetylcholine receptor chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TMEM35</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DoxX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919903.1">
        <property type="nucleotide sequence ID" value="XM_014064428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5K5"/>
    <dbReference type="GeneID" id="106547306"/>
    <dbReference type="KEGG" id="tsr:106547306"/>
    <dbReference type="CTD" id="59353"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030548">
        <property type="term" value="F:acetylcholine receptor regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000010">
        <property type="term" value="P:positive regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040399">
        <property type="entry name" value="TMEM35A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163:SF0">
        <property type="entry name" value="NOVEL ACETYLCHOLINE RECEPTOR CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163">
        <property type="entry name" value="SPINAL CORD EXPRESSION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013919903.1"/>
        </source>
    </evidence>
    <sequence length="172" mass="18753" checksum="9DA413AE9D977798" modified="2020-10-07" version="1">MEPALRIQESTLKGLEVKGLAFPGSQGFAAPTSLDETENENGPLKRAYKTYVRALPMLKRLGVSSIILRKSIGALEVACGIVMTLVPGRPKDVANFVLLLLVLAVLFFHQLVGDPLKRYAHALVFGILLTCRLLIARQPEDQPPEKRTVTVNGEEQQLLADAAPEKGKIKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6F8</accession>
    <name>A0A6I9Y6F8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM133B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FAM133B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM133 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920633.1">
        <property type="nucleotide sequence ID" value="XM_014065158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6F8"/>
    <dbReference type="GeneID" id="106547859"/>
    <dbReference type="KEGG" id="tsr:106547859"/>
    <dbReference type="CTD" id="257415"/>
    <dbReference type="OrthoDB" id="10065679at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026766">
        <property type="entry name" value="Fam133"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31911:SF1">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 133 MEMBER B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31911">
        <property type="entry name" value="PROTEIN FAM133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="14"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920633.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="28450" checksum="F59EF61773399AEA" modified="2020-10-07" version="1">MGKRDNRVAYMNPIAMARSRGPAPSSGPTIQDYLNRPRPTWEEVKEQLEKKKKGSKALAEFEERMNENWKKELEKHREKLLSGNESTSRKKEKKKKEKKKSSRLSPSSSSSSSSDSSSSSSDVEDEDKKQGKKRKKKKYHSLQKSSESSSSDSDSDSKDSLRTKRTKEDYERDKDSKSLVRKRIKADHMEELLPSDSSLESIETEEVQAKKKKNSEEKEKNVSLFYLSKNVKLQILYQHVLYIYIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7E6</accession>
    <name>A0A6I9Y7E6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Disintegrin and metalloproteinase domain-containing protein 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ADAM19</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915040.1">
        <property type="nucleotide sequence ID" value="XM_014059565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7E6"/>
    <dbReference type="GeneID" id="106543526"/>
    <dbReference type="KEGG" id="tsr:106543526"/>
    <dbReference type="CTD" id="8728"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018358">
        <property type="entry name" value="Disintegrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF19">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="23" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0800">Toxin</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="21">
        <location>
            <begin position="10"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="20">
        <location>
            <begin position="203"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="437"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="529"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="581"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="603"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="617"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="651"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013915040.1"/>
        </source>
    </evidence>
    <sequence length="702" mass="76787" checksum="E3A28A8FEFAFE48E" modified="2020-10-07" version="1">MAEFIFLLQFQKNKYDLQATKNKLVEAANYVDKFYRSFNIRIALVGLEIWNDYNKCDISANPHSTLWSFLAWRRRLLINKKHDNAQLITGMSFHGTTIGLAPLMAMCSSYQSGGVNMDHSENAIGVAATMAHEMGHNFGMSHDSVGCCSASPRDGGCIMAAATGAPFPRVFNECNRKELERYLQSGGGMCLYNMPDTRTLYGGQKCGNGYLEEGEECDCGEVEECQNPCCNAANCTLKQGAECAHGTCCHKCKLVSPGTLCREQERTCDLPEYCSGKSPFCPANFYQMDGTPCEDGRAYCYNGMCLTYEQQCLQLWGSGARPAPDLCFERVNAAGDSYGNCGKDISGNYRKCDTRNAKCGKIQCQSSASKPLESNAVAIDTTIKLNGRVIHCRGTHVYRNEEDEGDILDPGLVMTGTKCGNNHVCFEGHCQNTSFFDSGHCGKKCNGHGVCNNNHNCHCFSGWAPPFCEKPGKGGSLDSGPMLEKSPIPIIIGVLVSVLLLTLIGIAFCCYKWRAKLVPIKKAIFAPKKTEQSSIPPPVQKEVNGHANPAFKLKTPQGQRKITDIQNVLSKSEVLYCEPPAELKRQGKPPPPPVPANYAKKPPFLEHGTEEKHLPKRTPPNRPAPPPPKVMVQQDSSRLKPPQRALPANPVPSSHRTGLSKVNTGAIPPYGTLRNTGIQNAVLAENRATRNPGAANIQKFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8H5</accession>
    <name>A0A6I9Y8H5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Kunitz-type protease inhibitor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SPINT1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the venom Kunitz-type family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916935.1">
        <property type="nucleotide sequence ID" value="XM_014061460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8H5"/>
    <dbReference type="GeneID" id="106545015"/>
    <dbReference type="KEGG" id="tsr:106545015"/>
    <dbReference type="CTD" id="6692"/>
    <dbReference type="OrthoDB" id="2019384at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008544">
        <property type="term" value="P:epidermis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22623">
        <property type="entry name" value="Kunitz_HAI1_1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22624">
        <property type="entry name" value="Kunitz_HAI1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000061">
        <property type="entry name" value="Dyslexia-associated protein KIAA0319 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000006">
        <property type="entry name" value="Serine peptidase inhibitor, Kunitz type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013980">
        <property type="entry name" value="MANSC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011106">
        <property type="entry name" value="MANSC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46750">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46750:SF1">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22352">
        <property type="entry name" value="K319L-like_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07502">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00765">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50986">
        <property type="entry name" value="MANSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026725261" evidence="11">
        <location>
            <begin position="26"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="MANSC" evidence="13">
        <location>
            <begin position="46"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="12">
        <location>
            <begin position="247"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="12">
        <location>
            <begin position="371"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013916935.1"/>
        </source>
    </evidence>
    <sequence length="513" mass="57930" checksum="3CD645FF9C7FD96E" modified="2020-10-07" version="1">MGRSRSRLQAGLLVTWFFLTGLVLGEEHNGEGVSFGEMCLAQFTAGFPEFVLDTNASVENGATFLGSPPVESNRDCVRACCKNPNCNLALVEQSPGEDSDGIQSCFLLDCLYDQALVCRFAKKDGFLNYIRREVYNAYATMRNRDNRDDQPPKAKAWMDVRVQSKDEPVILNGSESTDDNGIVNYEWTLLSGDSSVVMEKQNDIMMLSNLNEGIYIFQFTVTDTADQQHSINITVTVLNSDQTVDHCLVPYKVGRCRGSFPRWYYNPEIEQCQQFIFGGCNPNKNNYVRKEECDLACKNVQGAVEKPQKPTCNGNCLPDYFKCEDGCCIEKILECDERLDCRDGSDEKSCDSYIQGFNKLQKINPSNRTYCVNKPDTGRCEESIPRWYYNPITEKCGRFTYGGCEGNKNNFEQEETCLKACSGITKEDMIGERWRAYEYQQASLSAFEVAVAVLLGVCIMIVLAVLAYFFLKNRKTYRRRRQSPTVANSTLSSVLSTAEDTEHLVYNSTTKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8I7</accession>
    <name>A0A6I9Y8I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SNX3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916995.1">
        <property type="nucleotide sequence ID" value="XM_014061520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8I7"/>
    <dbReference type="GeneID" id="106545063"/>
    <dbReference type="KEGG" id="tsr:106545063"/>
    <dbReference type="CTD" id="8724"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051223">
        <property type="term" value="P:regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07293">
        <property type="entry name" value="PX_SNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000002">
        <property type="entry name" value="Sorting nexin 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042137">
        <property type="entry name" value="PX_SNX3_Vert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF1">
        <property type="entry name" value="SORTING NEXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PX" evidence="15">
        <location>
            <begin position="27"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013916995.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18669" checksum="A00C7152C2259874" modified="2020-10-07" version="1">MAETVADTRCLITKPQNLNDAYGPPSNFLEIDVSNPQTVGVGRGRFTTYEVRVKTNLPIFKLKESCVRRRYSDFEWLRSELERESKVVVPPLPGKAFLRQLPFRGDDGIFDDSFIEERKLGLEQFINKVAGHPLAQNERCLHMFLQDEIIDKTYTPSKIRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8K3</accession>
    <name>A0A6I9Y8K3_9SAUR</name>
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        <submittedName>
            <fullName evidence="4">Netrin receptor DCC-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106545141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917075.1">
        <property type="nucleotide sequence ID" value="XM_014061600.1"/>
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    <dbReference type="GeneID" id="106545141"/>
    <dbReference type="KEGG" id="tsr:106545141"/>
    <dbReference type="OrthoDB" id="114660at2759"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013917075.1"/>
        </source>
    </evidence>
    <sequence length="156" mass="17396" checksum="2B227F574DD0FD3B" modified="2020-10-07" version="1">METISGDRLTHQIMELNLDTVYYFRIQARNAKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGAYWPVDTNLIDRSSLNEPPIGQMHPPHGSVTPQKNSNLLVVIVVTVGVITIIVVVIVAVICTRRSSAQQRKIFDKVSTRLSSTTTVSQYQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA45</accession>
    <name>A0A6I9YA45_9SAUR</name>
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        <submittedName>
            <fullName evidence="3">Regulating synaptic membrane exocytosis protein 1-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC106547226</name>
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        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Serpentes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of membrane potential"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:2000300">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039032">
        <property type="entry name" value="Rim-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157:SF18">
        <property type="entry name" value="REGULATING SYNAPTIC MEMBRANE EXOCYTOSIS PROTEIN 1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="38"/>
        </location>
    </feature>
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        <location>
            <begin position="102"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919785.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="28018" checksum="78D016FD4215C604" modified="2020-10-07" version="1">MRQPGSPMQSSPADTSFSRKGRQLPQVPIRSGSTEQANLVVEERTRQMKMKMHRYNQTTGCGSSQELEHEQYTKYNIQTDQYRSCDNVSAKSSDSDVSDVSAISRTSSASRLSSTSFMSEQSEHPRGRISTFTSKMQGGRMGTSGRIITKSTSVSGEVYKLEQNDGSQSDTAVGTVGTGGKKRRSSLSAKVVAMVSRRSRSTSQLSQTETGNKKLKSTIQRSTETGMAAEMRSRMVRQPSRESTDGSINSYSSEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAV8</accession>
    <name>A0A6I9YAV8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Pre-B-cell leukemia transcription factor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PBX2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
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            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
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            <taxon>Thamnophis</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/PBX homeobox family.</text>
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        <property type="nucleotide sequence ID" value="XM_014065625.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106548279"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pre-B-cell leukemia transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005542">
        <property type="entry name" value="PBX_PBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03792">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51978">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PBC" evidence="11">
        <location>
            <begin position="16"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="209"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="211"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="331"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="292"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007601"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921100.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="43218" checksum="554AEBF5C8ADFBC2" modified="2020-10-07" version="1">MTPHALHEPPSDNGEPRKQDIGDILQQIMTITDQSLDEAQAKKHTLNCHRMKPALFTVLCEIKEKTGLSIRSSQEEEPVDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAAAGGVSPDNSIEHSDYRNKLSQIRQIYHSELEKYEQACNEFTTHVMNLLREQSRTRPVSPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFSKQATEVLNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQEEANIYAVKTAVSAAQSGGDSPNTPSSAGSGGSFNLSSSGDMFMGLQGLNGDSYSTSQVESMRHSMGPGGYGDSMAANQMYSPREMRANGGWQEAATPSSVTSPTEGPGSVHSDTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB75</accession>
    <name>A0A6I9YB75_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">N-glycosylase/DNA lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">OGG1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921358.1">
        <property type="nucleotide sequence ID" value="XM_014065883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB75"/>
    <dbReference type="GeneID" id="106548502"/>
    <dbReference type="KEGG" id="tsr:106548502"/>
    <dbReference type="CTD" id="4968"/>
    <dbReference type="OrthoDB" id="238681at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.40:FF:000001">
        <property type="entry name" value="N-glycosylase/DNA lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="8-oxoguanine DNA glycosylase N-terminal" evidence="7">
        <location>
            <begin position="17"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013921358.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="27837" checksum="2F8578C2A7F739C3" modified="2020-10-07" version="1">MNPRPTQLPRATPWRSLPCPRTELRLDLVLCGGQTFRWSETSPGYWTGVLAGRVWTLTQSEEHLWYTLHEEEKEDGGREEEGLSEPPLKKSRKQTQPEPNRPGTSERPGSHLPGELARGTDGMTPSQILQDYFQLHVNVSGLYQGWSYVDSHFREIGVKFPGVRVLRQDPVECLFSFICTSNNHLSRITNMIQHLCQAFGRRLCQLDTKTYHAFPTLQAMAGKNLRPHSCFAAFPSTNHPTYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC35</accession>
    <name>A0A6I9YC35_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="15">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">USP13</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013921693.1">
        <property type="nucleotide sequence ID" value="XM_014066218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC35"/>
    <dbReference type="GeneID" id="106548770"/>
    <dbReference type="KEGG" id="tsr:106548770"/>
    <dbReference type="CTD" id="8975"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14384">
        <property type="entry name" value="UBA1_UBP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14386">
        <property type="entry name" value="UBA2_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000016">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000770">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000063">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF105">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8 15 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="18">
        <location>
            <begin position="183"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="17">
        <location>
            <begin position="332"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="16">
        <location>
            <begin position="657"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="16">
        <location>
            <begin position="732"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="831"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013921693.1"/>
        </source>
    </evidence>
    <sequence length="871" mass="98392" checksum="D1592907B5B75BFE" modified="2020-10-07" version="1">MERAALFAQGREAPMAAGDVGELLVPYMPTIRVPKTGDRVYKTECAFSYDSPDSEGGLYVCMNTFLGFGREHVERHYRKTGQCVYLHIKRHVREKVTGASGGALPKRRNTKLFLDLESNNDLNHDDYEYEDEAKLVIFPDHYEIPLPNIEELPALVTIACDAVLSSKSPYRKQDPDSWEEELQISKHANTLVQMDNGVRIPPSSWKCSKCDLRENLWLNLTDGSILCGKWFFDGSGGNGHALEHYKEMGYPLAVKLGTITPDGADVYSFEEEEAVLDPHIAKHLAHFGIDMLQMHVTENGLRDNDIKPRVSEWEVIQESGMKLKPMYGPGYTGMKNMGNSCYINSVLQAIFSIPEFQRAYVGNLPRIFDYSSLDPTQDFNTQMAKLGHGLLSGQYSKPPVKSELIEHVMKEQHKPQNNGISPRMFKAFISKGHPEFSSSRQQDALEFFLHLINLIERNRIGSENPSDVFRFLVEERTQCCQTRKVRYTERVEYLMQLPVAMEAAINKDELIAYELKRREAETARKPPPEVVRARIPFSACLQAFTEPENVEDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWIPKKLDVSIDMPDLLDITHLRATGFQPGEEELPDISPPVIIPVDPKGTAYYHEDSYLHTILHHNSPDFDESSVMQLAEMGFPLEACRKAVYYTGNMGAEMAFNWIIAHMEEPDFAEPLSIPGYGGTAFPQTGGSGVPGLDNQPPEEMVAIISSMGFQRNVALQALRATNNNLEHALDWIFSHPEPEEETEVASEVMNIENHVNANILAESDPEGPQIKDGPGRYELFGFISHMGTSTMSGHYVCHLKKEGRWVIYNDHKVCASEQPPIELGYIYFYHRIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCT8</accession>
    <name>A0A6I9YCT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho-related GTP-binding protein RhoE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Rho family GTPase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Rnd3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RND3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921874.1">
        <property type="nucleotide sequence ID" value="XM_014066399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCT8"/>
    <dbReference type="GeneID" id="106548902"/>
    <dbReference type="KEGG" id="tsr:106548902"/>
    <dbReference type="CTD" id="390"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04172">
        <property type="entry name" value="Rnd3_RhoE_Rho8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000407">
        <property type="entry name" value="Rho-related GTP-binding protein RhoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041843">
        <property type="entry name" value="RhoE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013921874.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="27440" checksum="825B127AB9F31B08" modified="2020-10-07" version="1">MKERRASQKLTSKSIMDPNQNVKCKIVVVGDTQCGKTALLHVFAKDCFPENYVPTVFENYTASFEIDTQRIELSLWDTSGSPYYDNVRPLSYPDSDAVLICFDISRPETLDSVLKKWKGEIQEFCPNTKMLLVGCKSDLRTDVSTLVELSNHRQTPVSYDQGANMAKQIGAATYIECSALQSENSVRDIFHVATLACVNKTNKNVKRNKSQRATKRISHMPGRPELTTVTTDLRKDKAKSCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEC3</accession>
    <name>A0A6I9YEC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import receptor subunit TOM70</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial precursor proteins import receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Translocase of outer membrane 70 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Translocase of outer mitochondrial membrane protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">TOMM70A</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922523.1">
        <property type="nucleotide sequence ID" value="XM_014067048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEC3"/>
    <dbReference type="GeneID" id="106549403"/>
    <dbReference type="KEGG" id="tsr:106549403"/>
    <dbReference type="CTD" id="9868"/>
    <dbReference type="OrthoDB" id="66418at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000141">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000071">
        <property type="entry name" value="Putative mitochondrial import receptor subunit TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 19" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="382"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="457"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="526"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="48"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013922523.1"/>
        </source>
    </evidence>
    <sequence length="589" mass="65817" checksum="98614069FC865186" modified="2020-10-07" version="1">MAASKPVDAAAGGNGTGSGIPRWQLALAIGVPLIGVGAFYWWNRRRRGTRAGGKGTSERKTPEGRASPAFTDSSGGSHLDGPAGGGDELSPLDKAQAAKNKGNKYFKAGKYEQAIQCYTEAISLCPSEKNSDLSTFYQNRAAAYEQLQKWKEVAQDCTKAVELNPRYVKALFRRAKAHEKLDNKKECLEDVTAVCILEGFQNQQSMLLADKILKLLGKEKAKEKYKNREPLMPSPQFIKSYFSSFTDDIISQPLQREEKSDEDKDKEGEASIIKENCGYLKAKKYMEEENYDKIISECTKEIEAKGKYMAEALLLRATFYLLIGNANAANPDLNQVIVMEDANVKLRANALIKRGSMYMQQQQPSLSTQDFNMAADIDPQNADVYHHRGQLKILLDRVEEAVEDFDECIRLRPDSALAQAQKCFALYRQAYTGNNTSQVQAAMKGFEDVIKKFPKCAEGYALYAQALTDQQQFGKADDMYDKCIDLEPDNATTYVHKGLLQLQWKQDLDKGLELISKAIEIDNKCDFAYETMGTIEVQRGNLDKAIDMFNKAINLAKSEMEMAHLYSLCDAAYAQTEVAKKYGLKPPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF78</accession>
    <name>A0A6I9YF78_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Layilin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106549638</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922817.1">
        <property type="nucleotide sequence ID" value="XM_014067342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF78"/>
    <dbReference type="GeneID" id="106549638"/>
    <dbReference type="KEGG" id="tsr:106549638"/>
    <dbReference type="OrthoDB" id="5797898at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789:SF2">
        <property type="entry name" value="LAYILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013922817.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25726" checksum="050960B33C7AC7BF" modified="2020-10-07" version="1">MYHQPSAPAGDGGRYLFQWNDDRCNMKNNFICKYSEEKPTVVPELAASQTGYITRSSAFTSPEPPMQEAVANKTLPEAKEPAWSLLYILISSIPALLLLITIAVFIFWVYAKRKREQREENTKEKDGWPSTKTRNSPSLEIYNVIKTQGEADLGGTRPQTQNSSFRVRLGGEDPCGDYDNMVVNNSESGFVTLASTESGFVTNDIYELCHDRVGPSKESAWVENEIYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFK0</accession>
    <name>A0A6I9YFK0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MAM domain-containing glycosylphosphatidylinositol anchor protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106549804</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923023.1">
        <property type="nucleotide sequence ID" value="XM_014067548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFK0"/>
    <dbReference type="GeneID" id="106549804"/>
    <dbReference type="KEGG" id="tsr:106549804"/>
    <dbReference type="OrthoDB" id="9023696at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000019">
        <property type="entry name" value="MAM domain containing glycosylphosphatidylinositol anchor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051560">
        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282">
        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF123">
        <property type="entry name" value="MAM DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAM" evidence="9">
        <location>
            <begin position="54"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013923023.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="28880" checksum="34A2C266DEA4FA66" modified="2020-10-07" version="1">MEKGMLQEYLLTDLKVPQSYELRLTPITRFGMGDIVTRIIHYIEPISYPNPVGNTCHFEDEKICGYIQDTTDNFDWIRQSSLTHDPKRTANTGPTMDFSGTPEGYYMFIEASAPRVKDDKARLISPMYNMTARFCVSFYYHMYGKHIGSLNLLVHVRNKQPTQALSLKGDKGNMWQQVHVPINPAGPFQIIFEGVRGTGSEGDIAIDDVTLKKGDCPRKPIVPNKAVALPGNSAVTQHTSFLCWPLLFFLYVLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFX9</accession>
    <name>A0A6I9YFX9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Gigaxonin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GAN</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923173.1">
        <property type="nucleotide sequence ID" value="XM_014067698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFX9"/>
    <dbReference type="GeneID" id="106549936"/>
    <dbReference type="KEGG" id="tsr:106549936"/>
    <dbReference type="CTD" id="8139"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18455">
        <property type="entry name" value="BACK_KLHL16_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18245">
        <property type="entry name" value="BTB_POZ_KLHL16_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030579">
        <property type="entry name" value="KLHL16_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047070">
        <property type="entry name" value="KLHL16_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF17">
        <property type="entry name" value="KELCH-LIKE PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="33"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923173.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="38918" checksum="070F5187D5066FCC" modified="2020-10-07" version="1">MSSGAPGSSVSDPQHAARLLRALSSFRQEGRFCDANLVLDGQEIPVQTNILAAASPYVRTKLNYNSPKNDGSVYKIELEGISVETMKEILDYIFSGQIKLSEETIQDVVQAADLLLLTDLKKLCCEFLEGCIAAENCIGIRDFALHYCLLHVHYLASEYLETHFRDVSSTEEFLELNPQRLKEVLSLEKLNVGDEKHIFEAVIRWISHDPELRKVHMKDVMSAVWVAGLDSTYLREQMMNEPLVREIVKECNNIPLTPPQQEEALLASFKPRGYSECIVTVGGEERVSRKPSAAVRCMCPLYDRHRQLWIELAPMSTARINHSVLSAEPNQTVVEMQMIDSIIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI66</accession>
    <name>A0A6I9YI66_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Epsin-3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">EPN3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923901.1">
        <property type="nucleotide sequence ID" value="XM_014068426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI66"/>
    <dbReference type="GeneID" id="106550499"/>
    <dbReference type="KEGG" id="tsr:106550499"/>
    <dbReference type="CTD" id="55040"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16990">
        <property type="entry name" value="ENTH_Epsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000002">
        <property type="entry name" value="epsin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF16">
        <property type="entry name" value="EPSIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ENTH" evidence="8">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013923901.1"/>
        </source>
    </evidence>
    <sequence length="627" mass="68272" checksum="A52E458DC33B1C4E" modified="2020-10-07" version="1">MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFAEVMGMIWRRLNDSGKNWRHVYKALTLLDYLIKTGSEKVAHQCRENLYTIQTLKEFQYIDRDGKDQGINVREKVKQVMSLLKDEDRLKQERAYALKTKERMSVEGMGISSSNSNSHQLAYGRRASQYGDDYSRSRGSPSSFNCEYCKASSSSPRFNSDLEQARPQTTGEEELQLQLALAMSREEAEKQPLPPVSSTDEDLQLQIALTLSKQEHEKEVKAWKGENSLLQKALEESNLGVEEVEEDDKGKKTQSSVLELADIFGPAPVTSTHTSADPWDIPDTKSPVESTGTSWGPSADPWVPLPSEDPLTHSSAGQASSSPWNLPPVSSASDPWGIKSLPSDNSTFDLFAKLPESVDQPEHESSQASSSVKANSSGDVDLFGDFIPSTAENGIQNKDIFDITSLGDSLPDSKDNKTPETFLGPNASSLVNLDSLVIVPQSVKSRNPFLAGLSAPSPTNPFNVDQPKPTLNQMRTNSPVPSVPMGMPMNSMTYSASLPLPLSSIPSTITLPASVSAFPQAGAGPFPELPGNLPQPLLPLSGSIPPPLASQSVPVSQSQIQSAKWWNSATSNPLQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI96</accession>
    <name>A0A6I9YI96_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Testis development-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TDRP</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924088.1">
        <property type="nucleotide sequence ID" value="XM_014068613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI96"/>
    <dbReference type="GeneID" id="106550669"/>
    <dbReference type="KEGG" id="tsr:106550669"/>
    <dbReference type="CTD" id="157695"/>
    <dbReference type="OrthoDB" id="9634112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031399">
        <property type="entry name" value="TDRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35663:SF1">
        <property type="entry name" value="TESTIS DEVELOPMENT-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35663">
        <property type="entry name" value="TESTIS DEVELOPMENT-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15683">
        <property type="entry name" value="TDRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924088.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="23926" checksum="2041DA94036E4854" modified="2020-10-07" version="1">MWKLNKSSKVLLDDSPEEEDGRRQERPPPPPPPPPPAGTFSAQTKNKDSCLQDEVTSSVSQLATKVQGAGFRGWKEVTSIFNKDDEQQLLTGCRSPKSKGTNMKLKEDLKSEKKGGFWDSLVIKQNLHSRKPDEIEGWDPPQIPAGDHLNDAENTLSDSSSWSGWEDETKGSTKYTNLASSGNNSSRWSIKSAGRLVSIRRQSKGNLTDNWEELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ14</accession>
    <name>A0A6I9YJ14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear envelope phosphatase-regulatory subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transmembrane protein 188</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CNEP1R1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNEP1R1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923944.1">
        <property type="nucleotide sequence ID" value="XM_014068469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ14"/>
    <dbReference type="GeneID" id="106550536"/>
    <dbReference type="KEGG" id="tsr:106550536"/>
    <dbReference type="CTD" id="255919"/>
    <dbReference type="OrthoDB" id="5786980at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071595">
        <property type="term" value="C:Nem1-Spo7 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019168">
        <property type="entry name" value="NEP1-R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996:SF1">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09771">
        <property type="entry name" value="Tmemb_18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923944.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15748" checksum="5AC95043A05B01DA" modified="2020-10-07" version="1">MNMNMDKLQEILEDEVGAWNLKAFERRLTEYIACLQPATGRWRIILIVVSVCTATGAWNWLIDPETQKVSFFTSLWNHPFFTISCITLIGLFFAGIHKRVVAPSMYPFPRVLCRYQVRMCAIHNYLICLNLQQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKM8</accession>
    <name>A0A6I9YKM8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Inhibin beta B chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106551106</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimeric or heterodimeric through association with alpha and beta subunits, linked by one or more disulfide bonds. Inhibins are heterodimers of one alpha and one beta subunit. Activins are homo- or heterodimers of beta subunits only.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924594.1">
        <property type="nucleotide sequence ID" value="XM_014069119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKM8"/>
    <dbReference type="GeneID" id="106551106"/>
    <dbReference type="KEGG" id="tsr:106551106"/>
    <dbReference type="OrthoDB" id="6516235at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19406">
        <property type="entry name" value="TGF_beta_INHBC_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000005">
        <property type="entry name" value="Inhibin beta A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001318">
        <property type="entry name" value="Inhibin_betaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF6">
        <property type="entry name" value="INHIBIN BETA E CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00672">
        <property type="entry name" value="INHIBINBC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="12">
        <location>
            <begin position="202"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013924594.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="36166" checksum="B3DB11972A63CC66" modified="2020-10-07" version="1">MPPLEPSAEKAFLVEVAKQQILQKLNLSERPNITHPVPRMAVANALRRLHMGKGWTDGMGHFSSWEKTESDEQGYEIISFAETEPSGLLQFQLTHTKDQAMHVLKAQLWLHLKAHENITIQIYVAGEPQVLLGQQQLGSQGSGWHTFSLMPALQNFFEHEDKTLRLELHCEGCHPNIMSLMATGSNSHQPFLVVKAKIKELGHRVAKRSLSCDQNSDLCCRKDYYVNFREIGWNDWIFKPEGYQINYCMGECPMHLAGVPGVAASFHTTVFNLVKANNIPIAGHSCCVPTQRRALSLLYFDPNSNLIKTDIPDMVVDACGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLM0</accession>
    <name>A0A6I9YLM0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Group 10 secretory phospholipase A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PLA2G10</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925132.1">
        <property type="nucleotide sequence ID" value="XM_014069657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLM0"/>
    <dbReference type="GeneID" id="106551534"/>
    <dbReference type="KEGG" id="tsr:106551534"/>
    <dbReference type="CTD" id="8399"/>
    <dbReference type="OrthoDB" id="10069378at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000001">
        <property type="entry name" value="Basic phospholipase A2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF4">
        <property type="entry name" value="GROUP 10 SECRETORY PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027161997" evidence="8">
        <location>
            <begin position="18"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="31"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="72"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="78"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925132.1"/>
        </source>
    </evidence>
    <sequence length="155" mass="17437" checksum="D55F189A32F98B4B" modified="2020-10-07" version="1">MAPPLVLPLLLLLLVQTGHPVAKAPSRPRRGILQLAGMVQCTTGRTPLAYIRYGCYCGWGGHGWPKDQVDWCCFKHDCCYGKAEEENCAPKMRRYPWECRDSKAKCDDIEDKCQKMACECDRSAAKCLAKAPYNVTYLFWPEAQCGERSPACPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YM15</accession>
    <name>A0A6I9YM15_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mas-related G-protein coupled receptor member H-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106551803</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family. Mas subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925433.1">
        <property type="nucleotide sequence ID" value="XM_014069958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YM15"/>
    <dbReference type="GeneID" id="106551803"/>
    <dbReference type="KEGG" id="tsr:106551803"/>
    <dbReference type="OrthoDB" id="9044473at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000193">
        <property type="entry name" value="Mas-related G-protein coupled receptor member E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026234">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334:SF68">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11334">
        <property type="entry name" value="MAS-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02108">
        <property type="entry name" value="MRGPCRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="59"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925433.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="35875" checksum="0568CA5D07DADE9D" modified="2020-10-07" version="1">MTTTFSGMMDSKMGNLTFNDTERNDNDAIHLESKMYLPGFLGTVIYSSIILICIPGIIGNGAVIHFLRCQIKRSHFTVYILNLAVADSVTLLFLFISAILHMAIKDTLIEITEVLVFTYCTGQLLLTIISIDRCLALFFPIWHRCHQPPYLSTILCAIAWIVSILFCGIHYTLILVKRRRKFSQFYHVFIYIAACIPLMIVSTLALLIKGYVKSQMQKRRKLLIVTLLSMFFFFIFSFPPIAIYVVNVLSSNQYFNLITLDYLCACLNSSVNPLIYFLLGRRKEHHSIYNLKGNLQTLFKEEEESEEKSEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YM42</accession>
    <name>A0A6I9YM42_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">C-X-C motif chemokine 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CXCL14</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925206.1">
        <property type="nucleotide sequence ID" value="XM_014069731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YM42"/>
    <dbReference type="GeneID" id="106551601"/>
    <dbReference type="KEGG" id="tsr:106551601"/>
    <dbReference type="CTD" id="9547"/>
    <dbReference type="OrthoDB" id="8667291at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0202">Cytokine</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026772866" evidence="2">
        <location>
            <begin position="23"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="3">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925206.1"/>
        </source>
    </evidence>
    <sequence length="98" mass="11716" checksum="BF1C975735D9B108" modified="2020-10-07" version="1">MKLLTAVLLILVLVICFTSVEGSKCKCLRKGPKIRLSDIQKLEERPKYPYCKERMIIVTMKSRFRGGHQYCLHPKLPSTKRLLKWYTIWKEKERVYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMI8</accession>
    <name>A0A6I9YMI8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myotubularin-related protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Inactive phosphatidylinositol 3-phosphatase 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106551598</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925204.1">
        <property type="nucleotide sequence ID" value="XM_014069729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMI8"/>
    <dbReference type="GeneID" id="106551598"/>
    <dbReference type="KEGG" id="tsr:106551598"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13211">
        <property type="entry name" value="PH-GRAM_MTMR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14536">
        <property type="entry name" value="PTP-MTMR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000240">
        <property type="entry name" value="Myotubularin-related protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048994">
        <property type="entry name" value="PH-GRAM_MTMR6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF56">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21098">
        <property type="entry name" value="PH-GRAM_MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="14">
        <location>
            <begin position="124"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925204.1"/>
        </source>
    </evidence>
    <sequence length="555" mass="64411" checksum="F2B687DFBE3210F4" modified="2020-10-07" version="1">MEFAELIKTPRADNVVLHRPFYPAVEGTLCLTGHHLIFSSRRQDNVEELWLLHSNIDSIEKRFIGFLGTIIIKCKDLRIIQLDIPGMEESLNIASSIEALSTLDSITLMYPFFYRPMFEVIEDGWHSFLPESEFELLNSVTNEWRLSYINKDFSVCPSYPPVVTVPKSIDDESLCKVATFRHGGRFPVLSYYHKKNGMVIMRSSQPLTGTNGRRCKEDEKLINATLRAGKRGYIIDTRSLNVAQQARAKGGGFEQEVHYPQWRRIHKSIERYNILQESLIKLVEACNDQSHNMDRWLSKLEASNWLTHIKEILTTACLAAQCIDREGASVLIHGTEGTDSTLQVTSLAQIILDPRCRTIRGFEALLEREWRQAGHPFQQRCAQSAYSNSKQKWEAPVYLLFLDCVWQILRQFPCSFEFNYHFLIMLFEHSYASQFGTFLGNNENERSKLKLSQKTMSLWSWVNRPEELNRFKNPLFEANSLVIWPSVAPQSLQLWEGVFLRWNRSSKFVDESYEEMINIIKYNKELQVKVNMLRRQLAELEIDDNTQDDGMPESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMV6</accession>
    <name>A0A6I9YMV6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Testis-specific serine/threonine-protein kinase 6</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">Serine/threonine-protein kinase SSTK</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Small serine/threonine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="primary">LOC106551948</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="35">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="26">Serine/threonine-protein kinase component of the sperm flagellar doublet microtubules. May act as a regulator of sperm motility by mediating phosphorylation of sperm doublet microtubule proteins. Plays a role in DNA condensation during postmeiotic chromatin remodeling and histone-to-protamine transition during spermatogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="27">Microtubule inner protein component of sperm flagellar doublet microtubules. Interacts with HSP90; this interaction stabilizes and activates TSSK6. Interacts with the heat shock proteins HSPCB, HSPA8 and HSPA1A. These interactions appear to be required for TSSK6 kinase activity. Interacts with TSACC; this interaction is direct and recruits TSACC to HSP90, which is essential for kinase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013925587.1">
        <property type="nucleotide sequence ID" value="XM_014070112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMV6"/>
    <dbReference type="GeneID" id="106551948"/>
    <dbReference type="KEGG" id="tsr:106551948"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000442">
        <property type="entry name" value="Testis-specific serine/threonine-protein kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF102">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 31 32" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="23" id="KW-0966">Cell projection</keyword>
    <keyword evidence="20" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-0221">Differentiation</keyword>
    <keyword evidence="18" id="KW-0282">Flagellum</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 31 32" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 32" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="33">
        <location>
            <begin position="16"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00053629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00065387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00071769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00079002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00080692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_013925587.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="31764" checksum="C6244AFA81BFB457" modified="2020-10-07" version="1">MSKSSGGDRWLNELGYKLGQTLGEGSYSKVRVATSAKYKGPLAIKVVDRRRAPPDFVHKFLPRELSILRAIRHPNIVRVFEFIEVCNGKLYIVMEAASTDLLQLVQQLGKLPCIPEARDIFLQVVSAVRYLHDRNLVHRDLKCENVLLTADGRRAKLTDFGFGRESRGYPDLSTTYCGSAAYASPEVLLGIPYDPKKYDVWSLGVVLYVMVTGCMPFDDSHIHSMPRRQKKGVFYPEGLPPLPEPCKSLIAQLLQFSPASRPGVGQVAKNTWLKGGTLSKEPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMX5</accession>
    <name>A0A6I9YMX5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable imidazolonepropionase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106552108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925768.1">
        <property type="nucleotide sequence ID" value="XM_014070293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMX5"/>
    <dbReference type="GeneID" id="106552108"/>
    <dbReference type="KEGG" id="tsr:106552108"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050480">
        <property type="term" value="F:imidazolonepropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005920">
        <property type="entry name" value="HutI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752:SF1">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013925768.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="16170" checksum="77300EA1F5B7DD29" modified="2020-10-07" version="1">MRSTPTKQTLLRALRPLRPLSGSCLGSATDEAMAGGEYKLLLEGAEQLVLVCTQGEQYLLEAGMEQLALRGNASVVIGKNGCIEAVGDARAIREQFSGATFDKIIDCSGKCILPGWYGFKDLLYQAVIYVFIEGQDMPLFLLLYFPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP47</accession>
    <name>A0A6I9YP47_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BRCA1-A complex subunit RAP80</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Receptor-associated protein 80</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin interaction motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">UIMC1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RAP80 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925480.1">
        <property type="nucleotide sequence ID" value="XM_014070005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP47"/>
    <dbReference type="GeneID" id="106551844"/>
    <dbReference type="KEGG" id="tsr:106551844"/>
    <dbReference type="CTD" id="51720"/>
    <dbReference type="OrthoDB" id="7536094at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20912">
        <property type="entry name" value="AIR_RAP80-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038868">
        <property type="entry name" value="RAP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040714">
        <property type="entry name" value="RAP80_UIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15932:SF2">
        <property type="entry name" value="BRCA1-A COMPLEX SUBUNIT RAP80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15932">
        <property type="entry name" value="UBIQUITIN INTERACTION MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18282">
        <property type="entry name" value="RAP80_UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RAP80 N-terminal" evidence="13">
        <location>
            <begin position="74"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="220"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="431"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="715"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="728"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925480.1"/>
        </source>
    </evidence>
    <sequence length="804" mass="89163" checksum="EAE1CD184DE41D36" modified="2020-10-07" version="1">MEKMAKKKRKTANEPDSKDQGEEEARDLSNAKLTHSADTFIIISDSDGEQESKEEHALQRKGTKQQLDRVKFTAKRKISLFSEMSEDEQFALALKMSEQEARQLNSQEEEEEELLRKAIAESLSSCQASAAAPVPLLSQASGSPVQSHPSEPEGSQLLAVSSPCSKSPCSDCSSLFPSDKIDENTETCVTKSPLVVLTRLSQDIVESSLLSSIIVTPGKSQTFARSGTSSPAGNCSSDTSHSSEELPAVLSPTFPQRPRRSWQLEPRRLFMGKCSLPTPTSKEIEHSQNYLKGNLHVDCPGVLQKEAQQQSTFDHDTSPSEFRDQAEHGKNTVLSAELTERTGLPESAPAICTLPRTDEKCQHEEPDVVHYYWGVPFCPKGIDPNKYTQVIMCQLEVYQKSLKQAQRQLLQKKPFGEPIVPNSCSLRQRLLAKDKETSRQREDTDDQEGEDTDEKKEPDNVPWLLSQVNGESYKHPAGDVAEGGDSEYGDEPASSSLQASQVLFADDMPEEREPVQITQSICALTPLDNTKNPDIATEDHAEEEITVCPETQQSPSQAVESESGEIHSSSKNVFLQADEDVREHAPGHSPQADNLLSCPLCELRFPASKIELHAMNCNGTGEDAVEDGPVMTRRQREAKSKLASCKKVCKSVDIDKYEKCYLCKSLVLRKEYQSHVDSCLYSWSVGGTQGHKRPCHAKEKGRCEGGLLRMLEQSENKSADAEMAAPPSRREDSRPSSTEMDKETEGNQNSERFLPQVPCSDSPIKSFTSISEAKDCLVDFKKQLAIKPRAWKQTKASHRNQKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQX2</accession>
    <name>A0A6I9YQX2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24 25" type="primary">LOC106552537</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24 25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates such as apomucin-, MUC5AC-, MUC1- and MUC2-derived peptides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Golgi apparatus membrane</location>
            <topology evidence="20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="RefSeq" id="XP_013926319.1">
        <property type="nucleotide sequence ID" value="XM_014070844.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926320.1">
        <property type="nucleotide sequence ID" value="XM_014070845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQX2"/>
    <dbReference type="GeneID" id="106552537"/>
    <dbReference type="KEGG" id="tsr:106552537"/>
    <dbReference type="OrthoDB" id="330637at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.460:FF:000001">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000014">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000005">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF123">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="18 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="22">
        <location>
            <begin position="426"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013926319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013926320.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="64166" checksum="5DD92B5B74BBFD0A" modified="2020-10-07" version="1">MRKFAYCKVVLATSLVWVLLDMFMLLYFSECNKCDEKKERGLPPGDVPESVQKPHEGPGEMGKPVAIAKEDQEKMKEMFKINQFNLMASEIIALNRSLPDVRLEGCKTKMYPDNLPTTSVVIVFHNEAWSTLLRTVHSVINRSPRYMLEEIILVDDASERDFLKRPLENYVKKLRIPVYVIRMEQRSGLIRARLKGAAASKGQVITFLDAHCECTVGWLEPLLARIKANRKTVVCPIIDVISDDTFEYMAGSDMTYGGFNWKLNFRWYPVPQREMDRRKGDRTLPVRTPTMAGGLFSIDRDYFQEIGTYDAGMDIWGGENLEISFRIWQCGGTLEIVTCSHVGHVFRKATPYTFPGGTGQIINKNNRRLAEVWMDEFKNFFYIISPGVTKVDYGDISSRLGLRQKLQCKPFSWYLENVYPDSQIPRHYFSLGEIRNAETNQCLDNMARKENEKVGIFNCHGMGGNQVFSYTANKEIRTDDLCLDVSKLHGPVTMLKCHHLKGNQLWEYDPVKLILLHVNSNQCLDKATEEDSQVPSIKDCNGSRSQQWLLRNVTLLEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSD3</accession>
    <name>A0A6I9YSD3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TIR domain-containing adapter molecule 1</fullName>
            <shortName evidence="6">TICAM-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TICAM1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Found in a multi-helicase-TICAM1 complex at least composed of DHX36, DDX1, DDX21 and TICAM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
            <location evidence="6">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The N-terminal region is essential for activation of the IFNB promoter activity.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926540.1">
        <property type="nucleotide sequence ID" value="XM_014071065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSD3"/>
    <dbReference type="GeneID" id="106552723"/>
    <dbReference type="KEGG" id="tsr:106552723"/>
    <dbReference type="CTD" id="148022"/>
    <dbReference type="OrthoDB" id="9906976at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032481">
        <property type="term" value="P:positive regulation of type I interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043330">
        <property type="term" value="P:response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035666">
        <property type="term" value="P:TRIF-dependent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025735">
        <property type="entry name" value="RHIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046946">
        <property type="entry name" value="TCAM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040886">
        <property type="entry name" value="TRIF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47230">
        <property type="entry name" value="TIR DOMAIN-CONTAINING ADAPTER MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47230:SF1">
        <property type="entry name" value="TIR DOMAIN-CONTAINING ADAPTER MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12721">
        <property type="entry name" value="RHIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17798">
        <property type="entry name" value="TRIF-NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4 6" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 6" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3 6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="8">
        <location>
            <begin position="375"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="196"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926540.1"/>
        </source>
    </evidence>
    <sequence length="660" mass="71745" checksum="69C549C3772904DF" modified="2020-10-07" version="1">MPFNLGPALPRMAGASQADGGFEGILQILDGIPAERLIQYRHKLSCLHQDRKSCHLLHIMILWSLGRDDEARVHLDSLRDDEVALHLSQSLWGTGGATRKTPEQLQKDLAKVAQSVAEIYSLLVDEKLCDPLARDEAYREAIRAFKASHLVGAQLKGLLDEAVEKCGLQFISTVPKKNVEALASGRWELTPARRSSLGPIVSSSPPDNPQSLRSTGSPVSYISDLKISESSITSNSPPSTGGSLPLPTTGQGENIRSLSLANSCSANSRKPSLPNSRGPGLLPHSKITQSPAASAQSPPTPNPASSGDLTRTFQSPSLQKPGSALSAGSGLPRSSAPAPITSFGPLLSSSSSTSDLASCSSEPSSSMVPPDDERQFYTFVVLHAAEDELVACRVKERLEALGVSNGATFSEDFLVPGRGQLNCFQNALDHSAFTLVLLTRNFTGRLCEYQRDTALMDSLTRCCKRNSVIPFVPKENSVNPVQMPFLLASLVPLCEKSPVFEKRVKKTFTPKVIREKKAAWDLLRQIQEREEARERERENLWLQQHLAVLNLQSSVQPPVVEMSRMQAQGGNDAQVFPPPATSFVKVGLDQASFSHSFLSSMGSPNTMPGPQLIIQNAQMIQIGDYNKMQVERSNPMVGTANETIERAPVAEKEDNSREWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSE6</accession>
    <name>A0A6I9YSE6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MICAL-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MICALL2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926555.1">
        <property type="nucleotide sequence ID" value="XM_014071080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSE6"/>
    <dbReference type="GeneID" id="106552731"/>
    <dbReference type="KEGG" id="tsr:106552731"/>
    <dbReference type="CTD" id="79778"/>
    <dbReference type="OrthoDB" id="10017054at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000112">
        <property type="entry name" value="MICAL like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF87">
        <property type="entry name" value="MICAL-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51848">
        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="151"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="BMERB" evidence="9">
        <location>
            <begin position="622"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="207"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="632"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="486"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013926555.1"/>
        </source>
    </evidence>
    <sequence length="800" mass="87615" checksum="42DF82F648084F86" modified="2020-10-07" version="1">ILHRHRPDLIDFTSLSKENVFENNALAFRVAEKELGIPALLDAEDMVTLRVPDRLSILTYVSQYYNYFHGKSPIGGMAGIKRPASEPIDQPTEKKGVPEPRAPSSTQQPARPPLPLEPKAEPAVVGRRMGMETSPTVERRVLAERNNAQPSNCAVCGTHVHLVQRLLMDGKLYHRNCFRCKKCSSTLQSGNYKAGISPGTFLCNRHAGADSAQAPPAQESRTEKKTASSKTPAGSGGKGLGPTPDASPVPKPRRAFQSNVTPQPAAAQEPSNSRSPKSGGVSPSPNLLRPHPPAPTPDPLVASPSTEVHSRIQQAREKFSQADPGTSDFPARKVEPAKSLSSPAQPVLILEKTPVSRTQGPVGSSDSKKDEARSVLQRALPGPQPGGVGLAAPRSLAHNSPSSPALSGLRSLQIQNPPASKPLNKPALADTPKGAETLHLPKDPSHKWGVVADPQNKIKDGSTGTSADAGRKWDTKVAKVKGPLGAEEKLESPEDWRSRLKPVKPPCQDGAKIPPKPTDVHKVEINVQVKPRQNDNRPSLSVLSPAVPNSAPLAVQPQTKKKLLASHLHDSSSWQKGKEPWESQPATGKAEKWSPSKTVVAPAQPSSPGTFWKALSEEATSPTKLPPDYVPEEEIQKEIRQIERDLDKLEQKGISLEKQLRACEGDDSEDALMVEWFKLIHEKQILLRRESELMHKINQQKLEVKQWDIETELRTLIGKADSLKTPKEKAREKELLDSLLDTVNNRNQIVEDLDEDRLREREEDEVFVNMIKKFGVSQSPQENEKKKKVFGFLRIPKAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUS0</accession>
    <name>A0A6I9YUS0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium voltage-gated channel subfamily V member 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106553981</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928052.1">
        <property type="nucleotide sequence ID" value="XM_014072577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUS0"/>
    <dbReference type="GeneID" id="106553981"/>
    <dbReference type="KEGG" id="tsr:106553981"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF65">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="15">
        <location>
            <begin position="72"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="231"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013928052.1"/>
        </source>
    </evidence>
    <sequence length="500" mass="57587" checksum="743790D41CC0D9EA" modified="2020-10-07" version="1">MKPAVPRRASFSANMKFRACQKCCRTPNDVDLFIPFSQEKLVKRWSSMDNLADLSQDGGRDLSHPWDPCFLSLNVGGQHFYIAVRAAARYPTTRIGQLATCVDPVKKLDLCDDYSVLRNEYFFDRDPSVFNYVFHFYRSGVMWVLTEFCSANFLQEIEYWGIHMKYAQRCCRILFEEKHDELVEYLKIQRELEAELETVEKEEHFKGKFLGRFRKAIWNLIENPYSSTLAKVTAILSSLCVLFSVVGMTLSTVKEMQSHTAKQAMEHMETACAIFFTLEYLMRVVSTSSVQQFFRAAFSAIDLVSILPFYIQVVFEYLGEEDAEDTEELHQMRNVGKLGKVLKLIKLMRIFRILKLARHSTGLRAFGFTLRQCCQPVCCLLLFIAMGIFTFSALMHSVEHDVPGTNFTSIPNTWWWAAVSLSTVGYGDTVPETFLGRVVAFGCIAFGIILNGMPISILYNKFSDYYAKLKSQEHKTTVALKTPRKQSLKERLWNKMNQCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUX9</accession>
    <name>A0A6I9YUX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myelin basic protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MBP</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Myelin membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the myelin basic protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928408.1">
        <property type="nucleotide sequence ID" value="XM_014072933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUX9"/>
    <dbReference type="GeneID" id="106554285"/>
    <dbReference type="KEGG" id="tsr:106554285"/>
    <dbReference type="CTD" id="4155"/>
    <dbReference type="OrthoDB" id="8862162at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043218">
        <property type="term" value="C:compact myelin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033269">
        <property type="term" value="C:internode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000548">
        <property type="entry name" value="Myelin_BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11429">
        <property type="entry name" value="MYELIN BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11429:SF0">
        <property type="entry name" value="MYELIN BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01669">
        <property type="entry name" value="Myelin_MBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00212">
        <property type="entry name" value="MYELINMBP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928408.1"/>
        </source>
    </evidence>
    <sequence length="356" mass="39511" checksum="91795E43B2536389" modified="2020-10-07" version="1">MLTIPPPHRSRRGPTEKKRRAGRGGGTKPASTREHLGSGRNVEIIMGNQVRKQDQITEKAKNTNANFEEAEHKDSSKPIPLTQEPSEENEVFGEADVNQNNGTSTKSRAVTDSKGTADPKNAWKEANPADTTSRPHLVRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAAAAASENSDLMASQKRSSFRHGSKLATASTIDHARHGSPRRYREPSLLDSLGRFFGGDKHYPRRGKDYHHASRVSHVSSLPHPRSYHGRYVDENPVVHFFRNIVSPRTTPPLQAKGRGLSFSRFSWGGETHRPGYGSGKFYEHKYAHKGHKGYRYDGQGTLSKLFRLGGSGSRPGSRSGSPVARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV88</accession>
    <name>A0A6I9YV88_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">B-cell antigen receptor complex-associated protein beta chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CD79B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928523.1">
        <property type="nucleotide sequence ID" value="XM_014073048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV88"/>
    <dbReference type="GeneID" id="106554400"/>
    <dbReference type="KEGG" id="tsr:106554400"/>
    <dbReference type="CTD" id="974"/>
    <dbReference type="OrthoDB" id="9894386at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019815">
        <property type="term" value="C:B cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030183">
        <property type="term" value="P:B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050853">
        <property type="term" value="P:B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14334">
        <property type="entry name" value="B-CELL ANTIGEN RECEPTOR COMPLEX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14334:SF2">
        <property type="entry name" value="B-CELL ANTIGEN RECEPTOR COMPLEX-ASSOCIATED PROTEIN BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013928523.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13474" checksum="D6F63F26E616FD46" modified="2020-10-07" version="1">MRINKLQHTDNGIYHCKNKDSLKATCGTELKVMGISTYEQVQSRHNVKDAIILIQTILLVLFVSIPVLLTQGKGDRKDASGEDHTYEGLTVELADTYEDIGVYQDRPEKWDLGEHPCEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVH2</accession>
    <name>A0A6I9YVH2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD repeat-containing protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">WDR20</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with USP12; promotes translocation of USP12/WDR20 to the plasma membrane. Component of the USP12/WDR20/WDR48 deubiquitinating complex. Interacts with USP46; contributes to the cytoplasmic localization of the USP46/WDR20 complex. Component of the USP12/DMWD/WDR48 deubiquitinating complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928613.1">
        <property type="nucleotide sequence ID" value="XM_014073138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVH2"/>
    <dbReference type="GeneID" id="106554470"/>
    <dbReference type="KEGG" id="tsr:106554470"/>
    <dbReference type="CTD" id="91833"/>
    <dbReference type="OrthoDB" id="3367at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000101">
        <property type="entry name" value="WD repeat-containing protein 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF5">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="645"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="408"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013928613.1"/>
        </source>
    </evidence>
    <sequence length="678" mass="74287" checksum="BE71B5B7AF135CD7" modified="2020-10-07" version="1">MLLSKMAAEGGGKEMNEIKTQFTTREGLYKLLSHSEYSRPNRVPFNSQGSNPVRVSFVNVNDQSGNGDRLCFNVGRELYFYIYKGVRKAADLSKPIDKRIYKGTQPTCHDFNHLTATAESVSLLVGFSAGQVQLIDPIKKETSKLFNEERLIDKSRVTCVKWVPGSESLFLVAHSSGNMYLYNVEHNCGTTAPHYQLLKQGESFAVHTCKSKSTRNPLLKWTVGEGALNEFGFSPDGKFLACVSQDGFLRVFNFDSVELHGTMKSYFGGLLCACWSPDGKYIVTGGEDDLVTVWSFVDCRVIARGHGHKSWVSVVAFDPYTTSVEENDPIEFSGSDEDFQDLLHFGRDRANSTQSRLSKRNSTESRPVSVTYRFGSVGQDTQLCLWDLTEDILFPHQPLSRARTHTNVMTATSPPTGSGGANPGSNGNSITTPGNSVPPPLPRSNSLPHSAVSNSGSKSSVMDGAIASGVSKFATLSLHDRKEKHHEKDHKRNHSMGHISSKSSDKLNLVTKTKTDPAKTLGTPLCPRMEDVPLLEPLICKKIAHERLTVLIFLEDCIVTACQEGFICTWARPGKVKAFHPVEAEGAAFAESVSAKMYLGEGSHHRIASSLSAAVSQLWKSVCIIILLEARNTGSTKPSSLEEEIIIIIIIIIIIIIIIIIIIIIAVAVIAVAVITIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVU8</accession>
    <name>A0A6I9YVU8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spermatogenesis-associated protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SPATA9</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928758.1">
        <property type="nucleotide sequence ID" value="XM_014073283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVU8"/>
    <dbReference type="GeneID" id="106554573"/>
    <dbReference type="KEGG" id="tsr:106554573"/>
    <dbReference type="CTD" id="83890"/>
    <dbReference type="OrthoDB" id="9442549at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031659">
        <property type="entry name" value="SPATA9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35669">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35669:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15824">
        <property type="entry name" value="SPATA9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928758.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="28800" checksum="9C3CB0588DA8F9F9" modified="2020-10-07" version="1">MALRPIGWICGEIVRKFAGQAGVIQRAILEVIDEIRDEFPNLLRLTRSNQVNYFSYNKLTIFVLNLKAFLFLFQETNPRVQKSESVITRVFYSAHGAIIMNGLTTLSQSSNSIAKVLQPQLVRKLTELNILSRRLLRTSNTPKQPLYKIKDKKVFFYDIISYPAKAALTSLMCASYAALIFLAVSINKLLAKLKGIIETEDTSRESSSEDVDENGFPKDPEATSPLQQDSSILPIKVSQKSGSIHTLLSAVDQESEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWX7</accession>
    <name>A0A6I9YWX7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PVALB</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928110.1">
        <property type="nucleotide sequence ID" value="XM_014072635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWX7"/>
    <dbReference type="GeneID" id="106554036"/>
    <dbReference type="KEGG" id="tsr:106554036"/>
    <dbReference type="CTD" id="5816"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16254">
        <property type="entry name" value="EFh_parvalbumin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF2">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013928110.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12602" checksum="8C52BDB5C973CE75" modified="2020-10-07" version="1">MAMADLFAGEEIKKMLRAFAATNSFDYKKFFEMLGLKNKSLQELKMFFSILDTDKNGYLEPGEIRVMLQIFSPNSRRLTEKETQEFMGAGDKDQDGKIGIDEFIVLVNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY08</accession>
    <name>A0A6I9YY08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Activating signal cointegrator 1 complex subunit 3</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
            <ecNumber evidence="14">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">U5 small nuclear ribonucleoprotein 200 kDa helicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106554348</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EC" id="5.6.2.4" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013928475.1">
        <property type="nucleotide sequence ID" value="XM_014073000.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554348"/>
    <dbReference type="KEGG" id="tsr:106554348"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18019">
        <property type="entry name" value="DEXHc_Brr2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000254">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="21" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="300"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="494"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="1147"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013928475.1"/>
        </source>
    </evidence>
    <sequence length="1376" mass="157626" checksum="31313B6D6F0FFD96" modified="2020-10-07" version="1">MDDNIDETYGVNVQFESDEEEGDEDVYGEVRDEASDEDLEGDEAVVRCTLSANLVASGELMCSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCSLLASAQSEAEKERIMGKMESDPELSKFLYQLHETEKEDLIREERCRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFAQGSHFMANKRCQLPDGSFRKQRKGYEEVHVPALKPKPFGSEEQLVPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDMNLLLCAPTGAGKTNVALMCMLREIGKHINLDGTINIDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEINATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEALVARTIRNIEMTQEDVRLVGLSATLPNYEDVATFLRVEPTKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQIYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVARLPDMLNAEIVLGNVQNAKDAVNWLGYTYLYIRMLRSPNLYGISHDDMKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNETIQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFEGLYQDKFPFFNPIQTQVFNTVYNSDENVFVGAPTGSGKTICAEFAILRMLLQNVEGRCVYITPMEALAEQIFLDWYEKFQERLLKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNVNLFIVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATATFNFHPNVRPVPLELHIQVSLPLFQMGSSDHAELLLQIGPLIKLPLWSPSLIDRSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZA1</accession>
    <name>A0A6I9YZA1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homeobox protein Hox-D8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HOXD8</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929642.1">
        <property type="nucleotide sequence ID" value="XM_014074167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZA1"/>
    <dbReference type="GeneID" id="106555335"/>
    <dbReference type="KEGG" id="tsr:106555335"/>
    <dbReference type="CTD" id="3234"/>
    <dbReference type="OrthoDB" id="9219420at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050948">
        <property type="entry name" value="Antp_homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46166">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46166:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929642.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="15488" checksum="0A9CCC45CF5A51E2" modified="2020-10-07" version="1">MSSYFVNPLYSKYKAAAAAAAGGGEPPINPAYYVCHFAPARPTIFTTHQEAELLQYPDCKSSCANIGEEPRPLNQSASPSPVFPWMRPQGWTQTPCLSPFPLPFSACFPKQFTTLSLEFLHLSTPADSGEYGSHPPYLEIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZU1</accession>
    <name>A0A6I9YZU1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106555487 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106555487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929827.1">
        <property type="nucleotide sequence ID" value="XM_014074352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZU1"/>
    <dbReference type="GeneID" id="106555487"/>
    <dbReference type="KEGG" id="tsr:106555487"/>
    <dbReference type="OrthoDB" id="10645543at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013929827.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="28688" checksum="F166E9FC1DB244D1" modified="2020-10-07" version="1">MEKRRRRLKLNSQAGTKKREEEEGGTFRGPFPSSLLTGAPLSPLLLQRGGRSRCGHPGGSHPPGRRADRLVRRNGSGRGRAHRGTRRKRDFGCQKATKQEHTTLGPSNGHKPGPDPCEHVPDGGHQPRPGERPRSGGYGACDCQDHRQHRHHGRPGGGSGHAGTVMADRGWIVTPQLLPLFSDFSTPLRKCNQEALGVSQLWTTQPRAEKGARARWVIKRTHLLPTGARRLVRTGYAPAFFAWIARSEEGRGSESFPALCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z067</accession>
    <name>A0A6I9Z067_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger protein 24-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106555607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929962.1">
        <property type="nucleotide sequence ID" value="XM_014074487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z067"/>
    <dbReference type="GeneID" id="106555607"/>
    <dbReference type="KEGG" id="tsr:106555607"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000005">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="48"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013929962.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="23405" checksum="C54BC356C6013E0B" modified="2020-10-07" version="1">METQLLGSEGNGKGRFAVQPGSCGEIWTKRGQKILEEETIPHSEVQPRNFSSLQYQEAEGPRGLCSRLHDFCRRWLRPEKHTKDQMLDLLVLEQLLVLLPLEMESWVRECGAETSSQAVALVEGFLLSQAEEQKEQVELQSVVKIRDPEGWRNPSNPLQEMFWRRISQEDPSQDISGVKNRMKITPPYDGTETIFEPITQVRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0H3</accession>
    <name>A0A6I9Z0H3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Neuronal PAS domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106555451</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929784.1">
        <property type="nucleotide sequence ID" value="XM_014074309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0H3"/>
    <dbReference type="GeneID" id="106555451"/>
    <dbReference type="KEGG" id="tsr:106555451"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990513">
        <property type="term" value="C:CLOCK-BMAL transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19736">
        <property type="entry name" value="bHLH-PAS_PASD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000013">
        <property type="entry name" value="Circadian locomoter output cycles protein kaput"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047230">
        <property type="entry name" value="CLOCK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001067">
        <property type="entry name" value="Nuc_translocat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46055:SF4">
        <property type="entry name" value="CIRCADIAN CLOCK PROTEIN PASD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46055">
        <property type="entry name" value="CIRCADIAN LOCOMOTER OUTPUT CYCLES PROTEIN KAPUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00785">
        <property type="entry name" value="NCTRNSLOCATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="85"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="160"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="334"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013929784.1"/>
        </source>
    </evidence>
    <sequence length="484" mass="54625" checksum="2445947ED5E76EE0" modified="2020-10-07" version="1">MTRLPKELPRLPGWQLKRAAFFFGQARFQPPPPPPPPLPLRLAPKVKPEIVAPSFVAAGAGSGSGDARTLNSLNELMDEDEKDRAKRASRNKSEKKRRDQFNVLIKELCTMLQGHGHPLKMDKSTILQRTIDFLQKQKEISAQTEACEIRQDWKPSFLTNEEFTQLMLEALDGFLIALTTDGIIIYVSDSVSSLLGHLPSDLVDQNILNFLPECEQNNVYKLLSAHVLVTSDFLNAEKQMEFCCHLARGNLDPNEAVTYEYVKFVVDFKQFSPAPASSFNGFEPPVARAFRSVSDTQVCLVATVRLVTPQFLKELCNMEDSCEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHADDLELLARCHEHLMQFGKGKSCYYRFLSKGQQWIWLQTHYYITYHQWNSKPEFIVCSHTVVSSSVATSQGDYINAQHCVSANQEVSREGVSVSSHSSCRSSHTALSDSACMAHTSSTQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0H5</accession>
    <name>A0A6I9Z0H5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Latent-transforming growth factor beta-binding protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106555458</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929789.1">
        <property type="nucleotide sequence ID" value="XM_014074314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0H5"/>
    <dbReference type="GeneID" id="106555458"/>
    <dbReference type="KEGG" id="tsr:106555458"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929789.1"/>
        </source>
    </evidence>
    <sequence length="136" mass="14535" checksum="D42E479C82247096" modified="2020-10-07" version="1">MTLIGENGHAMDTLTGSGFRVVVCPLPCMNGGQCTSGNHCLCPPEFTGRFCQMPVNRQGQQARPPSDGAPMETGSSKHAIYAVQVMSDHHSDSPSGPGSKGTVSHSTFMVPLGHHTSEAVTPAMVSVRWTEELWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0L6</accession>
    <name>A0A6I9Z0L6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oligoribonuclease, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RNA exonuclease 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Small fragment nuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">REXO2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides. Binds and degrades longer oligonucleotides with a lower affinity. Plays dual roles in mitochondria, scavenging nanoRNAs (small RNA oligonucleotides of &lt;5 nucleotides) that are produced by the degradosome and clearing short RNAs that are generated by RNA processing. Essential for correct initiation of mitochondrial transcription, degrading mitochondrial RNA dinucleotides to prevent RNA-primed transcription at non-canonical sites in the mitochondrial genome. Essential for embryonic development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the oligoribonuclease family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929834.1">
        <property type="nucleotide sequence ID" value="XM_014074359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0L6"/>
    <dbReference type="GeneID" id="106555491"/>
    <dbReference type="KEGG" id="tsr:106555491"/>
    <dbReference type="CTD" id="25996"/>
    <dbReference type="OrthoDB" id="270189at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06135">
        <property type="entry name" value="Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000003">
        <property type="entry name" value="Oligoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00045">
        <property type="entry name" value="Oligoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003765">
        <property type="entry name" value="PRK05359.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046:SF0">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="10">
        <location>
            <begin position="11"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013929834.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="23657" checksum="EB1E190A9BE99ED2" modified="2020-10-07" version="1">MAEAAGLMGQRIVWVDLEMTGLDIEKDQILEMACLVTDSGLNILAEGPNLIINQPDELLDGMSDWCKEHHGKSGLTKAVKESTVSLRQAEYEFLSFIRQQTPPGVCPLAGNSVHADKKFLDKHMPQFMKHLHYRIIDVSTVKELCRRWYPEDYEAAPKKSAAHRALDDIRESIKELHFYRNHIFKRRTDEKKRKLLENGESEQPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0Y0</accession>
    <name>A0A6I9Z0Y0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106555232</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013929500.1">
        <property type="nucleotide sequence ID" value="XM_014074025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0Y0"/>
    <dbReference type="GeneID" id="106555232"/>
    <dbReference type="KEGG" id="tsr:106555232"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF10">
        <property type="entry name" value="GAMMA-ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase N-terminal" evidence="11">
        <location>
            <begin position="82"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="10">
        <location>
            <begin position="221"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013929500.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="40644" checksum="52BBFF0C42A808DD" modified="2020-10-07" version="1">MSYLYSQCKTFMALPTTHILSFTLFTLFVHLILFLTEEVFDGNRDAKDHKMKISKLTHCLPFSSWAFYFYSPPGNPISTMSIEEIRAREILDSRGNPTVEVDLYTSKGMFRAAVPSGASTGIYEALELRDNDKSRFLGKGVLQAVDHINSTIAPALVNSAFSVVEQEKIDNLMLELDGTENKSKFGANAILGVSLAVCKAAAAEKDVPLYRHIADLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRIGAEVYHNLKSVIKDKYGKDATNVGDEGGFAPNILENSEALELLKEAIEKAGYTDKIVIGMDVAASEFYRDGKYDLDFKSPDDPSRYISADELGDLYQSFVRDYPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1V3</accession>
    <name>A0A6I9Z1V3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 281</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ZNF281</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930592.1">
        <property type="nucleotide sequence ID" value="XM_014075117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1V3"/>
    <dbReference type="GeneID" id="106556141"/>
    <dbReference type="KEGG" id="tsr:106556141"/>
    <dbReference type="CTD" id="23528"/>
    <dbReference type="OrthoDB" id="9899552at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000067">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000042">
        <property type="entry name" value="Zinc finger protein 148"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000313">
        <property type="entry name" value="Zinc finger protein 281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF155">
        <property type="entry name" value="EARLY GROWTH RESPONSE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="312"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="555"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="699"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="567"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013930592.1"/>
        </source>
    </evidence>
    <sequence length="815" mass="89108" checksum="5ADD924988EF3A00" modified="2020-10-07" version="1">MTFKKEPTGGFPLTASSSSSSSSQRGPWGFLHSLVNIKQEKPSEQEEEEQQQQHHHHHHHHYGGLFGIAGEERHPALGGGGGGGGGVEGANPSVIQDLSLFHHLHHHPHRGDLLLGIRSSGDLSAAGLDEHKQDAHGKKTKRPKCESQGNKAKKKPSASAKALVAGDGEAAMLSPSQKPHVCEHCNAAFRSSYHLRRHVLIHTGERPFQCSQCNMGFIQKYLLQRHEKIHSREKPFGCDQCSMKFIQKYHMERHKRTHSGEKPYKCETCQQYFSRTDRLLKHRRTCGEAIGKAGAGMDPGPSNHSMGNLSMLSQGNTGSSKRKSKAKCIPTENKEHKTSHKISESQIASNMSLQNYAVEIPIVSSSGGLIGAGLEELQKKVPKLIFKKGNRKSADKSFLNFISPLPDLLGQKQFSGKPSSSLGLVANSSADAIGLLQAASGKSGQISSNYDDAMQFSKKRRYLQTASSNSAFSINVGHMASQPSVIQSAGVSVMDNETPLSLIDPASLNADIKSCHDKSGIPDEVLQSLLDQYSHKSEGQKEDPFSITEQRVDLQASGEHPEMVQEENLSPSSQAASNDKASMLQEYSKYLQHAFERTTNSTGFAFGPSFQFVSLSSSLHNHTLFPDKQVYTTSPLECGFSQSVTSVLPSALSKPPFGMLLGSQPGFYLSALEATHQQLTPSQELDDLIDSQKNIDASSGYHATPQKLNSQKEQKTLETSTSFPIPSQELANQIDPQKDLEHRATYQIETFAQAFGSQFKTGSRVPMTFIANSNGEVDHRVRTSVSDFSGYSNIMSDVSEPCSTRVKTPTSQTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2F0</accession>
    <name>A0A6I9Z2F0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homeobox protein DLX-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DLX4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930404.1">
        <property type="nucleotide sequence ID" value="XM_014074929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2F0"/>
    <dbReference type="GeneID" id="106555975"/>
    <dbReference type="KEGG" id="tsr:106555975"/>
    <dbReference type="CTD" id="1748"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000266">
        <property type="entry name" value="Distal-less homeobox 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF26">
        <property type="entry name" value="HOMEOBOX PROTEIN DLX-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="149"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="151"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930404.1"/>
        </source>
    </evidence>
    <sequence length="277" mass="30400" checksum="EC3BC8A657B9D9D4" modified="2020-10-07" version="1">MNSLAESLLGPPDPSKAAFLDFGLGAPTSPHQQPQTSQPQQHPTTLAAPYSLQALHPLPPPPQQTPHDGGVFPSPAASYGSRPLAYPPYPGAAGGSPHAFYHHHHHGTSFSTYQQTSSTLGHGTRLEETELEKSTIIENGEIRINGKGKKIRKPRTIYSSLQLQALNQRFQQTQYLALPERAELAAQLGLTQTQVKIWFQNKRSKFKKIMKQGSSIQEDNLHSSSSLSPCSPNIPPLWDIPMACKGPPLPSNTYINSFGPWYQQPQHQDAMGRPPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2S0</accession>
    <name>A0A6I9Z2S0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fermitin family homolog 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106555782</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930165.1">
        <property type="nucleotide sequence ID" value="XM_014074690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2S0"/>
    <dbReference type="GeneID" id="106555782"/>
    <dbReference type="KEGG" id="tsr:106555782"/>
    <dbReference type="OrthoDB" id="10057618at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033622">
        <property type="term" value="P:integrin activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007159">
        <property type="term" value="P:leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033632">
        <property type="term" value="P:regulation of cell-cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01237">
        <property type="entry name" value="PH_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000057">
        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037837">
        <property type="entry name" value="PH_Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF1">
        <property type="entry name" value="FERMITIN FAMILY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="231"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930165.1"/>
        </source>
    </evidence>
    <sequence length="402" mass="45729" checksum="C85852884EA19291" modified="2020-10-07" version="1">PAVAIRHPEELSLLRAPEEKDKRKQKEKEETTAQSYDLTSVRLPASAEQQTFRGMPAHFSDSAETEGCYRMLSVSQTSVTPEQIVRMHRPGSLVEKTRINSRWLDSSRTLLQQGVKEQECLWLRFKYYSFFDLEPKVNAVRINQLYEQARWSVLLEETECTEEEMIVFAALQYRINQLSLNATPAEQSSESGLDDLEKALANLEVKLEGPDSTLNVLDSLTAIPELKDHLRIFRPRKLTLKGYKQYWVTFKETTISYFKSAEDALGEPVQQLNLKGCEVVPDVNISGQKFCIKLLIPSPDGMSEIHLRCAEEQQYASWMAGCRLAAKGKTMADSSYQAEKENILSFLKLQKTNPEMSLTAETEGTQWLMSPRYQKKFKPKQVSFVLQAAVVISHNPPGGRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2X4</accession>
    <name>A0A6I9Z2X4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Major histocompatibility complex class I-related gene protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106556126</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930574.1">
        <property type="nucleotide sequence ID" value="XM_014075099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2X4"/>
    <dbReference type="GeneID" id="106556126"/>
    <dbReference type="KEGG" id="tsr:106556126"/>
    <dbReference type="OrthoDB" id="9940220at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07698">
        <property type="entry name" value="IgC1_MHC_I_alpha3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF242">
        <property type="entry name" value="MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-RELATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0490">MHC I</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="3"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013930574.1"/>
        </source>
    </evidence>
    <sequence length="152" mass="17452" checksum="C8F35E0B8C1BDD49" modified="2020-10-07" version="1">MEPPVGKVTHKLVDDSLEVLICQAYGFYPKEIQATWTRDGENWEQETFRRTVAPNSDGTYYVWISIEIDPKERDRFSCHLEHDALQEPLVLACQEETATGWLIPVGIVAVLILGAVILFLRYWRRRTIQEAMALQDDFPLEGFMLRPSEGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2Y7</accession>
    <name>A0A6I9Z2Y7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho-related GTP-binding protein RhoG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">RHOG</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in immunological synaptic F-actin density and architecture organization. Regulates actin reorganization in lymphocytes, possibly through the modulation of Rac1 activity. Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Binds phospholipids in an activation-dependent manner; thereby acting as an anchor for other proteins to the plasma membrane (PM). Plays a role in exocytosis of cytotoxic granules (CG) by lymphocytes/Component of the exocytosis machinery in natural killer (NK) and CD8+ T cells. Promotes the docking of cytotoxic granules (CG) to the plasma membrane through the interaction with UNC13D. Involved in the cytotoxic activity of lymphocytes/primary CD8+ T cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with ARHGEF26. Interacts with ARHGEF16. Interacts with UNC13D; the interaction increases RhoG affinity to the membrane lipids, targets UNC13D to membrane lipids and facilitates cytotoxic granule (CG) docking to the plasma membrane.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930230.1">
        <property type="nucleotide sequence ID" value="XM_014074755.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013930232.1">
        <property type="nucleotide sequence ID" value="XM_014074757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2Y7"/>
    <dbReference type="GeneID" id="106555842"/>
    <dbReference type="KEGG" id="tsr:106555842"/>
    <dbReference type="CTD" id="391"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000118">
        <property type="entry name" value="Rho-related GTP-binding protein RhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00069400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013930230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930232.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21168" checksum="76122790278C6290" modified="2020-10-07" version="1">MQTIKCVVVGDGAVGKTCLLISYTTNAFPEEYIPTVFDNYSAQMTVDGRMVSLNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIGSPSSYANVRHKWHPEVSHHCPNVPILLVGTKRDLRSNAEAVKKLKEQSLGPTTPQQGTSLAKQIGAVKYLECSALNQEGVREVFAEAVRAVLYPVTKKNSKKCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z378</accession>
    <name>A0A6I9Z378_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch-like protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106556654</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931107.1">
        <property type="nucleotide sequence ID" value="XM_014075632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z378"/>
    <dbReference type="GeneID" id="106556654"/>
    <dbReference type="KEGG" id="tsr:106556654"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18449">
        <property type="entry name" value="BACK_KLHL9_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18239">
        <property type="entry name" value="BTB_POZ_KLHL9_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000002">
        <property type="entry name" value="Kelch-like family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000011">
        <property type="entry name" value="Kelch-like family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF11">
        <property type="entry name" value="KELCH-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="66"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931107.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="56068" checksum="FCA26A32127C3E19" modified="2020-10-07" version="1">MMWRDSLSLVEEEEPHMKVTLGSSDMGLSAHLQASKAGTTRFFTSNTHSSVVLQGFDQLRVEGLLCDVTLVPGDGDEVFPVHRAMMASASDYFKAMFTGGMKEQDLMCIKLHGVNKIGLKKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVSLENCVEVGRIANTYNLTEVDKYVNNFILKNFPALLSTGEFVKLPFERLAFVLSSNSLKHCTELELFKAACRWLRYEEPRMECAAKLMKNIRFPLMTPQDLINYVQTVDFMRTDNTCVNLLLEASNYQMMPYMQPVMQSERTAIRSDSTHLVTLGGVLRQQLVVSKELRMYDEKAHEWRSLAPMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHLSALKGHLYAVGGRNAAGELATVECYNPRMNEWSYVAKMNEPHYGHAGTVYGGLMYISGGITHDTFQKELMCFDPDTDKWTQKAPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5F8</accession>
    <name>A0A6I9Z5F8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PDE4D</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013931444.1">
        <property type="nucleotide sequence ID" value="XM_014075969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5F8"/>
    <dbReference type="GeneID" id="106556924"/>
    <dbReference type="KEGG" id="tsr:106556924"/>
    <dbReference type="CTD" id="5144"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="327"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="651"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="703"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="720"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="403"/>
            <end position="407"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="561"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="561"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931444.1"/>
        </source>
    </evidence>
    <sequence length="751" mass="85305" checksum="C5FE14AC59B3A814" modified="2020-10-07" version="1">MSKDTNSLSVRSKSASEETLYHQNEEEMALQGTDQYRVRRLSCRNLQLPPLAFRQLEQLDWEKKADPEMVQRPTNLPLRTLPLIAITAADTVSFDVDNGTSSGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLPDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPKFGVKTDQEDILSKELEDVNKWGLQVFKIAELSGNRPLTVVMYTIFQERDLLKTFKIPPDMLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQKQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPSSDDQEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDTKTLCTQDSESTEIPLDEQVGEEVVEEEEEENQSEPCVLEERSPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z639</accession>
    <name>A0A6I9Z639_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein SOGA3-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106557418</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013932142.1">
        <property type="nucleotide sequence ID" value="XM_014076667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z639"/>
    <dbReference type="GeneID" id="106557418"/>
    <dbReference type="KEGG" id="tsr:106557418"/>
    <dbReference type="OrthoDB" id="10036174at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049885">
        <property type="entry name" value="MTCL1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027881">
        <property type="entry name" value="SOGA_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742">
        <property type="entry name" value="GIRDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742:SF2">
        <property type="entry name" value="PROTEIN SOGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11365">
        <property type="entry name" value="SOGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="228"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="348"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="450"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="298"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013932142.1"/>
        </source>
    </evidence>
    <sequence length="700" mass="79899" checksum="A4FD4C6EF55CC3B0" modified="2020-10-07" version="1">MQSPGGDLDGGAPSAAAAASSSPSSSTPSPMAPGGVGEGNGRGRAPDGVPSMNPRRPQELLEEVEKLREENDTLKNEIDEMRTEMDEMRDSFFEEDACQLQEMRHELERANKNCRILQYRLRKAERKRLRYAQTGEIDGELLRSLEQDLKVAKDVSVRLHHELENVEEKRTTTEDENEKLRQQLIEVEITKQALQNELDKMKEQSMKRRGSKDLQKSEKKQQTPTEEDNEDLKCQLQFVKEEATLMRKKLAKIDKEKDRFEHDLQKYRSLYGDLDSPLPKGEAGGPPTTREAELKLRLRLVEEEANILGRKIVELEVENRGLKAELDDLRGDDFSGAANPLMGEQNESLSELRQHLQLVEDETELLRRNLADVEEQNKRITAELNKYKYKTGTHDTSRHHDSAKTEALQEELKATRLQINELSGKVMQLQYENRVLMSNMQRYDLASHLGIRGSPRDSDAESDAGKKESDDDSRPPHRKREGPVGGESDSEEVRNIRCLTPTRSFYPTPTGWQKSFTDRQQMKDIRCEAERLGKTIDRLISDTSTIITEARIYVANGDLFGLMDDEDDGSRIREHELLYRINAQMKTFRKELQAFIDRLEVPKSSDERSADEPLSVSQIKSARVVVSVAIAAVKTLKTICLKTMGTTLTFQNVDNKRNQKTNIPTTESDFCLSEKALSFPELEAHAPCLSYYFMFELKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z698</accession>
    <name>A0A6I9Z698_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PH and SEC7 domain-containing protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106556860</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Ruffle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931355.1">
        <property type="nucleotide sequence ID" value="XM_014075880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z698"/>
    <dbReference type="GeneID" id="106556860"/>
    <dbReference type="KEGG" id="tsr:106556860"/>
    <dbReference type="OrthoDB" id="2157641at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF338">
        <property type="entry name" value="PH AND SEC7 DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="4">
        <location>
            <begin position="538"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="692"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013931355.1"/>
        </source>
    </evidence>
    <sequence length="887" mass="98981" checksum="C61A5ECCB954357B" modified="2020-10-07" version="1">MVNARMEDPERSPISDVMDRSSDDRTKEVSSDSGECLKQTWDLLAFSGTCQAGNAATPPPLALGEDRLEGVHCRNEPLSEKSIPVLMQNNFEVLHPELGHPVSQSLDFSERFGQPLEPIENLKTQDKCSNDATQSVFWAGILQAQMCFLDLQEELDRQKEPLQTCGVNSESLDTAIIDEPLLLESDTEEEPEENLEKDEEESEEEEEEEEVECLFFNNPLFQESPHLTRTFEGQVPWSQPERETSTENYEKKVKSEVDAGNHNGALFSQANAAMSPVARNYFSVCDPDANEHTSSYSFPSYVPHYRSFSPLLITEKDLEITCSLAEDSEDEDLPREDALGSLTGSPPRRCFLDPLPGEVLESLPPEPSAYLPAEGLLFSASSTSLMSALLQEPTKSYPPAQPTPPGGSCCITSNFQVPSAPAAEQNLQQERSEPQASPHGHPSSSCIWLEEPERTSSLQKDVSGADVCSIPLCGVERKRGGSVERLKMVELPAMQSDGRPKGGEEEPMLYCPEEPAGEFGRTGGSLPAKVPAEPNGTLLLANGTSGDLAGAHRLAARLYHLDGFRKSQVAAFLRKNNTFSQMVAEAYLSFFRFSEQTLDQALRSFLKSFVLTGETQERERILMHFSQRYHCCNPEASLSPDAVHTLTCAMMLLNTDLHGQDEQHLEMSGSEELIGVSHALAEKASKYTKRPHVFRLQTADWHIFLFQAPTPEEMTSWISRINLVAAMFSAPPFPAAVGSQKKFIRPILPITPCKNSIEDQHLAHESCMDRVAHELMELQRNLPEKRGRGRDLEEYQLRKEYLLYEKRRYETYVKLLEVKLGCSSDDLDQWEVRLAAMVESCPSLQKSHSSPSLNVEGPPAGGAKVKRNISERRTVRRIIPKRNRHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WVV7</accession>
    <name>A0A6I9WVV7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fas-binding factor 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106537448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907054.1">
        <property type="nucleotide sequence ID" value="XM_014051579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WVV7"/>
    <dbReference type="GeneID" id="106537448"/>
    <dbReference type="KEGG" id="tsr:106537448"/>
    <dbReference type="OrthoDB" id="8195456at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090162">
        <property type="term" value="P:establishment of epithelial cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033561">
        <property type="entry name" value="FBF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049390">
        <property type="entry name" value="FBF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33689">
        <property type="entry name" value="FAS-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33689:SF1">
        <property type="entry name" value="FAS-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21007">
        <property type="entry name" value="FBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fas-binding factor 1 C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907054.1"/>
        </source>
    </evidence>
    <sequence length="202" mass="24095" checksum="1DB05152EA7B359A" modified="2020-10-07" version="1">MEDERGRLQEVISKMEARLNEQTRLLEQERWRVTTEQAKVESLQRSLEEQRRVMTQQLTMEREELERAKNSLLEEQKAVMQKCAEERRKVAAEWSELHIQQRLSKERSEREVDRALQIDSQREGAIMSLAKEQADLKIKSSELRSREEQLVRDRELLEQEKQELRLEKERVNAAALHVKQRADEINSMSKVKKLPFDIHYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYD8</accession>
    <name>A0A6I9WYD8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein phosphatase 2C-like domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PP2D1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907176.1">
        <property type="nucleotide sequence ID" value="XM_014051701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYD8"/>
    <dbReference type="GeneID" id="106537538"/>
    <dbReference type="KEGG" id="tsr:106537538"/>
    <dbReference type="CTD" id="151649"/>
    <dbReference type="OrthoDB" id="343114at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF837">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="155"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="660"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013907176.1"/>
        </source>
    </evidence>
    <sequence length="776" mass="88344" checksum="0BE1205157B84E9E" modified="2020-10-07" version="1">MTYQTELQNSEISEEEQKLQSSESFKEEQDLTKVLNIDFKKIESLNIKIPCSICEEMVYISQLFHHKKVHQAQAVLNYQWPFAEPINIKKIILRRKQLILRLKKTAQYPEREIEKIGCSIDLLKESIKTSPYFCIDNIVQSSAYIKEVSNPLIKGLAICQDKNAVWHTEMEDVFIVLDNYGHKKDTCLLGLFDGNNGISAAQLTSDELPLLLLEQLSHNDSSYQISDAEKNMLHSFCTVFKEDYEVKEQVFAVAKAVSQNSQPNKYEWIHRAFAKAFWRMDRLLCLGRGEVSKVRWSSCTTAICLVEMASNIKQNQKQQEGEETVETINDNEEQNVMQHEKGLPKTNRMIRSILEQEVEKIMEQQEENLLGRVSDGEGQNNIEQEEDSETELHQEEPLTKICHGKGQSNTEQEAGSLTEHPRELFTSANNGMKKSIIEDNEQPANRKDELQKEGWIENNENSDEIYGIMHIANIGNVHAVLCKNGKSYWLTKEHSTYCREERRRILQNGGSISSNEPKGLIEGLIKNTRGLGHHGNPKIKKTVIPVPNTISFSADNSCQFLIIATNGLWEVLDKSEVVLLTITLFSAYLAKYQHAQLMKRYIPNASKLSLEDQVYSWYLNRDFFNESDLGNEEEIESHFQVKVPINNNTEEKEVIQSKSRTISTKQLSENSLATPVNVPTSSKENTPPEVTKKESNKNLEDLISENHEFLQISEDSETNSGTFYNLAAKYITKHLVKAALKAGSRNNVSVLLALLNGCDNVPTDVLMGKTESKTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYS1</accession>
    <name>A0A6I9WYS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylyl cyclase-activating protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Guanylate cyclase activator 1A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GUCA1A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907291.1">
        <property type="nucleotide sequence ID" value="XM_014051816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYS1"/>
    <dbReference type="GeneID" id="106537599"/>
    <dbReference type="KEGG" id="tsr:106537599"/>
    <dbReference type="CTD" id="2978"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120199">
        <property type="term" value="C:cone photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008048">
        <property type="term" value="F:calcium sensitive guanylate cyclase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000083">
        <property type="entry name" value="guanylyl cyclase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054446">
        <property type="entry name" value="CIMIP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF13">
        <property type="entry name" value="GUANYLYL CYCLASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22581">
        <property type="entry name" value="CIMIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0844">Vision</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="119"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="162"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013907291.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="26546" checksum="FE816DB77C3418DC" modified="2020-10-07" version="1">MAQEEKGTEALLKSSDPAFKPQKKCPAALGYKMEQALISEYFPQVIYSGGFKPECLSFAFYNPNYSNSYNPFYTLQKPTCGYRYCRDTDHRRKVMDIERDGYIDFMEYVAALSLVLKGKVEQKLKWYFKLYDVDGNGCIDRDELLNIIKAIRTINPCQEVMSAEEFTDMVFNKIDVNGDGDLSLEEFIDGVQKDEVLLDILTRSLDLKHIFDKIQNDGKNPETTGEPTKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0J7</accession>
    <name>A0A6I9X0J7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ran-specific GTPase-activating protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ran-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RANBP1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L. Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN. Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1. Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with RAN (via C-terminus of GTP-bound form) but not with GDP-bound RAN. Identified in a complex composed of RAN, RANGAP1 and RANBP1. Identified in a complex that contains TNPO1, RAN and RANBP1. Identified in a complex that contains CSE1L, KPNA2, RAN and RANBP1. Identified in a complex with nucleotide-free RAN and RCC1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RANBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907297.1">
        <property type="nucleotide sequence ID" value="XM_014051822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0J7"/>
    <dbReference type="GeneID" id="106537635"/>
    <dbReference type="KEGG" id="tsr:106537635"/>
    <dbReference type="CTD" id="5902"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046604">
        <property type="term" value="P:positive regulation of mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000824">
        <property type="entry name" value="Ran-specific GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF94">
        <property type="entry name" value="RAN BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="10">
        <location>
            <begin position="27"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013907297.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="24218" checksum="387E8BE2EB4D0623" modified="2020-10-07" version="1">MAETKQEAHEEHDSATDNADDSNHDPQFEPIVSLPEQEIKTLEEDEEEVFKMRAKLFRFASESDLPEWKERGTGDVKLLKHKDKGTIRLLMRRDKTLKICANHYITPVMELKPNAGSDRAWVWNTHADFADESPKPELLAIRFLNAENAQKFKAKFEECRGEVDKKGKKAETDKNDSADKIAEKLEDLSVKDECKESEKKEGKEKTEEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X126</accession>
    <name>A0A6I9X126_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription factor IIIA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZNF76</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908046.1">
        <property type="nucleotide sequence ID" value="XM_014052571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X126"/>
    <dbReference type="GeneID" id="106538151"/>
    <dbReference type="KEGG" id="tsr:106538151"/>
    <dbReference type="CTD" id="7629"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004334">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001102">
        <property type="entry name" value="Transcription factor IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000236">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="167"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="227"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="257"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013908046.1"/>
        </source>
    </evidence>
    <sequence length="401" mass="44734" checksum="C93933A79CB27FD6" modified="2020-10-07" version="1">MESLSLQAMTLSDGTTAFVQQEADEEKLVDGQVIELEDGSTAFFQQVTLQKESITFEDGQPVTLEDGTMAFIHHTPKEGFDPNTLEAIQLEDGSTAFIHRPVPTTMDDTILAVEADVGLEELAVGKKDDAFDLDTGITLKDYTSKEDFQKEEVQSTGNGPRTPDKAYCCRYSGCSRLFTTSHHLKVHERVHTGDRPYQCDFTSCGKTFATGYSLKSHMRTHTGEKPYKCQEDLCTKAFKTSGDLQKHIRTHTGEKPYACMVPGCGKCFTEYSSLYKHHVVHTHCKPYTCNTCGKTYRQTSTLAMHKRTVHGELEAMDDSEQALYEHQLEDTIVNNSRPPLKSHCIVSMKMEDEVQEDSMPSAAALVSQDGVEQLEMQQSLESTISMITTPVHQEAKLKNGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1G4</accession>
    <name>A0A6I9X1G4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin carboxyl-terminal hydrolase MINDY</fullName>
            <ecNumber evidence="11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">FAM188A</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the MINDY deubiquitinase family. FAM188 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013907660.1">
        <property type="nucleotide sequence ID" value="XM_014052185.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907662.1">
        <property type="nucleotide sequence ID" value="XM_014052187.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907663.1">
        <property type="nucleotide sequence ID" value="XM_014052188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1G4"/>
    <dbReference type="GeneID" id="106537904"/>
    <dbReference type="KEGG" id="tsr:106537904"/>
    <dbReference type="CTD" id="80013"/>
    <dbReference type="OrthoDB" id="9981542at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000315">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase MINDY-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025257">
        <property type="entry name" value="MINDY-3/4_CD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039785">
        <property type="entry name" value="MINY3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13898">
        <property type="entry name" value="MINDY-3_4_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01174">
        <property type="entry name" value="DUF4205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Deubiquitinating enzyme MINDY-3/4 conserved" evidence="12">
        <location>
            <begin position="8"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013907660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013907662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013907663.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="50009" checksum="7D00E8307A6EFCB8" modified="2020-10-07" version="1">MADLEQMIPLVWGKKTSQGLADTIFCRWTQGFVFSDSESTALEQFEGGPCAVIAPVQALLLKKLFTCEKSTWRECQEEEQKNILCHALCEILEMTCSDQSESYSLATWQRGNPVEDTSNISESSAESSNQEEQSSALAVEEFGFERFHALIYKRSYANFSDFKDAVWNHHTMWTNKFGVLLFLYSVILTKGIENIKNEIEDSSEPLIDPVYGHGSQSLINLLLTGFAVSNVWDGDRECSGMKLLGINKQGAVGFLTLMESLRYCKVGSYLKSPKFPIWILGSETHLTVFFAKDVGLVAPEAPSEQARRVFQTYDPEDNGFIPDPLLEDVMKALDLVSDPDYVNLMKTKLDPEGLGIILLGPFLQEFFPEQDPKVQESFAVYHYNGLKQSNCNEKVMYVEGTAVIMGFEDPMLQTDDTPIKRCLQTKWPYIELLWTSDRSPSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2A0</accession>
    <name>A0A6I9X2A0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Homeobox protein Hox-C13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HOXC13</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907957.1">
        <property type="nucleotide sequence ID" value="XM_014052482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2A0"/>
    <dbReference type="GeneID" id="106538087"/>
    <dbReference type="KEGG" id="tsr:106538087"/>
    <dbReference type="CTD" id="3229"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000130">
        <property type="entry name" value="Homeobox protein Hox-D12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051003">
        <property type="entry name" value="AP_axis_regulatory_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022067">
        <property type="entry name" value="HoxA13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804">
        <property type="entry name" value="SEGMENTATION PROTEIN FUSHI TARAZU-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12284">
        <property type="entry name" value="HoxA13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="234"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="236"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013907957.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="34429" checksum="54D9CB8218396090" modified="2020-10-07" version="1">MTTSLILHPRWADTFMYVYEENPNENSQTKIPAMEGLSGNCTTSHCRDLITHPVLSRHSGTIGTHQGSVYTEIPAPEAARQCPAPPASSNATLGYGYPFGGSYYGCRLSHSHNVNLQQKPCAYHPAEKYPEPSGSLPSEELSSRAKEFAFYPSFASSYQAVPGYLDVSVVPGISGHPESRHDALLPMEGYQHWALSNGWDGQVYCAKEQSQSTHLWKSPFPDVVPLQPEVNSYRRGRKKRVPYTKIQLKELEKEYAASKFITKEKRRRISATTNLSERQVTIWFQNRRVKEKKVISKSKTSHLHAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4F7</accession>
    <name>A0A6I9X4F7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibroblast growth factor</fullName>
            <shortName evidence="2">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FGF21</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908737.1">
        <property type="nucleotide sequence ID" value="XM_014053262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4F7"/>
    <dbReference type="GeneID" id="106538689"/>
    <dbReference type="KEGG" id="tsr:106538689"/>
    <dbReference type="CTD" id="26291"/>
    <dbReference type="OrthoDB" id="5987799at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027161761" description="Fibroblast growth factor" evidence="2">
        <location>
            <begin position="33"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908737.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24777" checksum="24B142FAE85B589A" modified="2020-10-07" version="1">MLSVSVLLQRSGYFLLPINLLFWAVLVPRTVASPVPDSNPLYQFDGQVRLRHLYTANERTQLHLEIFSDGTVRGAKYQNAFSLMEIKAVKLGIIRILATKTSRFLCMDSEGHLYGSLSYSEQECNFREMVLRDGYNVYYSEAYNLPVSLSMMENLSQNRQLPPFSQFLPLVNKVPLEPMIMDYEYIQQVHDIESADPFNIMGQNQDLRSPSFAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4Y5</accession>
    <name>A0A6I9X4Y5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transforming protein RhoA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RHOA</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909311.1">
        <property type="nucleotide sequence ID" value="XM_014053836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4Y5"/>
    <dbReference type="SMR" id="A0A6I9X4Y5"/>
    <dbReference type="GeneID" id="106539135"/>
    <dbReference type="KEGG" id="tsr:106539135"/>
    <dbReference type="CTD" id="387"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000095">
        <property type="entry name" value="Rho-related GTP-binding protein RhoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013909311.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="21782" checksum="C4CFCDC31FF858BC" modified="2020-10-07" version="1">MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5E0</accession>
    <name>A0A6I9X5E0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Lysyl oxidase homolog 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106538911</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909027.1">
        <property type="nucleotide sequence ID" value="XM_014053552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5E0"/>
    <dbReference type="GeneID" id="106538911"/>
    <dbReference type="KEGG" id="tsr:106538911"/>
    <dbReference type="OrthoDB" id="547291at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004720">
        <property type="term" value="F:protein-lysine 6-oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000022">
        <property type="entry name" value="lysyl oxidase homolog 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050912">
        <property type="entry name" value="LOX-like_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817:SF2">
        <property type="entry name" value="LYSYL OXIDASE HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027061992" evidence="6">
        <location>
            <begin position="23"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="41"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="165"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="303"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="67"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="80"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="328"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="341"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013909027.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="48150" checksum="ABE58A143022D9D3" modified="2020-10-07" version="1">MRLVQRPLLGFLCGPWLWVSLAQPPSRAPTRQSPPSPNLRFRLAGYPRKHNEGRVEVFYNQEWGTICDDDFTLANAHILCRHLGFVAATGWTHSAKYGKGVGRIWLDNVSCGGHEKSIAECQSRGWGNSDCSHEEDAGVVCKDERIAGFTDSNVIEAEQNQVEEVRLRPVVSQAQRPLPVTEGIVEVRHKSSWAQICDENWGSQESRVICGMLGFPAERKANRNFYKLYLQRQQHQFRLHSVACAGDEVHLSACPFEFYKGNVTGACKGGMPAVVSCVPGPLFAGGNAQKKRRGQQPQRQLSVRLKGGAKTGEGRVELLKNGEWGTICDDRWNLLSASVVCRELGFGSAKEALRGARMGQGMGQIHLSEVQCLGPERSLWSCPHKNITQQDCRHSEDAAVRCNVPYMAHETTVGWAEERGVGRGDNHALLLFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X616</accession>
    <name>A0A6I9X616_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Splicing factor 3B subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SF3B4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins. Part of the SF3B subcomplex of the 17S U2 SnRNP complex. SF3B associates with the splicing subcomplex SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). SF3B4 has been found in complex spliceosome 'B' and 'C' as well. Component of the minor (U12-type spliceosome) spliceosome. Found in a complex with PRMT9, SF3B2 and SF3B4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910889.1">
        <property type="nucleotide sequence ID" value="XM_014055414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X616"/>
    <dbReference type="GeneID" id="106540337"/>
    <dbReference type="KEGG" id="tsr:106540337"/>
    <dbReference type="CTD" id="10262"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12335">
        <property type="entry name" value="RRM2_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000059">
        <property type="entry name" value="splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000141">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034159">
        <property type="entry name" value="SF3B4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="100"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="207"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="232"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="317"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910889.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="43892" checksum="B070DA781FF8CA35" modified="2020-10-07" version="1">MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADAPPPPSVPTPVVTTLGPGVTPPGIPPPGSFPPPVPPPGALPPGLPPSMPPPPMPPGAGAPGPPSAGAPGGAHPPHPHPFPPGGMHPGMPQMQVHHGMPGMGPHHPGPPGAGGQPPPRPPPGMPHPGPPPMGMPPRGPHFGSPMGHPGPMPPHGMRGPPPLMPPHGYNGPPRPPPYSYQRIPLPPRPAQRPMVPPRGPMRGPLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7E1</accession>
    <name>A0A6I9X7E1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Telomere-associated protein RIF1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RIF1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910176.1">
        <property type="nucleotide sequence ID" value="XM_014054701.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539808"/>
    <dbReference type="KEGG" id="tsr:106539808"/>
    <dbReference type="CTD" id="55183"/>
    <dbReference type="OrthoDB" id="5399929at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14267">
        <property type="entry name" value="Rif1_CTD_C-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022031">
        <property type="entry name" value="Rif1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928:SF3">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12231">
        <property type="entry name" value="Rif1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomere-associated protein Rif1 N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1197"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1318"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1440"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1851"/>
            <end position="2106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2142"/>
            <end position="2165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2259"/>
            <end position="2309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1204"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1336"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1356"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1440"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1475"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1499"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1521"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1613"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1691"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1710"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1747"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1766"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1782"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1892"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1921"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1940"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1993"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2070"/>
            <end position="2083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="2144"/>
            <end position="2155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2272"/>
            <end position="2283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2284"/>
            <end position="2301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013910176.1"/>
        </source>
    </evidence>
    <sequence length="2512" mass="278107" checksum="60ED078B078195C9" modified="2020-10-07" version="1">MGPCDGSSMRALLETLEDPSASPGVLTDAWLTLTNRLTEEEEDEDEGKEFAAEVENCFPQLYKLFMTHIPTENLELSSAALQTLGFCVCNPKIASLLCEIEIHELLSLLNSVAINSGDKHIRTRALWVISKQFFPPDTVGKLVPNIIAMLETILKGDSHSMVVEHEALNVIIRLLEQAPSDMEEQAVTWAKMIIPLLVHSAHKVQLKAASALEMGLPLLQQRQEVIAVAEQLLTTKVIPELQKLFFSKHETYILKLWPLFVKLLGKTLHHSGSFINSLLHLEELGFRSGSPVVKKIAFIAWKSLIDNFALNPDILCSAKRLKLLMQPLTSIHVRTETLALTKLEVWWYLLMRLGSQLPVHFEQVCVPLIQNTLSLDASLLQENSSRLISNQNIAIPGCSRKSGVFPFGNVANPRMTLNSSNEGAVIPSIQLLGIEMLLHFFMGPEVLTFAKENNIVLSLEPLQYPVITSPSFFCKYASVFINAAQDGFIAIGKDVSDFMLTAIWRELVGFVKAAIDSGNKRDRQHSEVLTMLLQTLKNIILSKELPVLNSLPLLEVAIKELPPKVLGSPAYQIADMDLLNGTPALFLVQLLFCEDLLKFGVENKRFFLNYETLVECTLSGPASPLAFTQSVLTIVSESALHFENKEYVWRMWSIIVNPLIECIKQTNEVNQGDALEHNFNAIYCALLLPVNCIFSSSEFPEFTMKKLLRSWSELYKAFARCTLLVPTADDNVCCEELCAKIISGLKDEERLNQSMLDGLSHITAVMVECINFAPYDVKFPLAHKPPQNLVEWARREKNPLGKMSSLIKLLIILMNSLQMLISEEFDCEPFAPLGFMIVGILHNIIGRVSLPSMLRPVLRFIAKPFAALYEKIQSCNQPSKLANPLSPGLNNKCEKLLADSLTCFQTHYPGSFDDEVLGEIFPLLSVMFSNRNRLVCSHAVSFWNATFGKARTMEYPEELKPILNTTRQKFILLLPNFESCEIMEGSSTTYSDYTENSQWGASISGIKVNPNGKRDSFLARTGEPEEENIDFHITPKKIKLELSGTKSNIKNIPLEEENTVDFVYIPVETKKRVLTEHQKEVLRTKRDDIPVMYNNLDVSQDVLFSQDTQNQEESLEIPIIVDVKQEHEKKEDKSTPQKKEATSLVIPHGVTGNYKSNISLENITSTVSEQPNSFQKENLDLKNEELNICVPIQSPILDSTLNENPPTSSSNSSASSDIISATPQVVVNRRQAFVTLEKFDDAESRPFSPSVFSNITVTLTNPFIQNESPVVSDVSSKDKPNLENKGRVKFTARKTMPGTRRTRSEFLNALEMCVKAKRIKEENSPDPKLQPNNTDDGLRQNKTLDDKASEQIQSDQEEKNQVSDTPCEDKHEISVDTQIAEHSLDNQSQIPGAGAADSTTMESKVLLDIVKNENVVPNLEHKENTATEVDVREEVVPDVNQSPDISLNQKTLRRSLRRKAETDGAVDSQDNENNQQKKDNEKDDEKIMPKKMAQNKETSGQKEKAETLEKAADAPICVPINNATENINSKENSQEELDMSLGKSERSPAASDLEESGPDTSVEKHKVFPRYHTRRAASQGLLASMENSESDISETREEGAKKRRSGKLKKNDSPGNQAKPDSQELSSQAAVEIQTRSVGKQKITSSGKTEVPSMSNNAKDIKQDKSTPPLSPCVDLKENAGLTRPRASSDTGRKAANRRESTSEPVLENGQETSALDTSSVVPINSNDISEDHASATAVGALPSPKRILDMSECQHKRSKRTRKSRSCECCDEKLSPQVEKLSSEPKKASTPEPKLKEPRKNLSKVTVALTLIPVAEEIHFAQRLNVEPCATSTPLPFVKDAADWKEASNVAKSVSGVPRNKSPNVEPEKLACVQSENDKPVAGIPDVPFSETISESRSMDSQSEMLSAQGQPLEPGSVDSHSEMLRAKDQDLEPGPMDSHSEEVSAKDQPLEPGSVDSHSEMLSAKDQPLEPGPVDSHSEEVSAMDQPLEPGRMDSHSEEVSAKDQPLESGSVDSHSEESVMDQGLEPDSMDSCSEESVMDQGLEPGSMDSHSEESVMDQGLEPGSMDSHSEEVSAKDRGLEPDAEAETGMEVCSNQNGHLESETDTEIKIIQVVGAQPEESVVQNTVEETAVTSATQVAELQENETSEEEGAEDNAANAPSQPSTLVMWQNKISTLDSPPKCKELDFLSLAKVSGSPTGTQARCLWSPSASPSTSILKRAIKREPEEDSSSPANKNRRVSFANPIYQEELADDIDRRSPILRIHPCNNGSQSSRSAKSSSSHQAKLITTPTKGSLSPGTRSHKYKSSKKCLITEMGKESTSPVPTESVYPALMGCKASVDIILPQITSNTWTRGLGHLIRAKNIRTVGDLSSLTAAEIKTLPIRSPRVLNVRKVLKGYHEQQVKSRGMEENTGLDNTEKSEIHVMDDTFFSSNENADQCEPEVSSSGESSAIDLWTQVNLLADQLSSERLRNYSGNELFEMQEKLHSMTDCIMKTLKTRWMLSRSCETTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X806</accession>
    <name>A0A6I9X806_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SRPK3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013909947.1">
        <property type="nucleotide sequence ID" value="XM_014054472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X806"/>
    <dbReference type="GeneID" id="106539646"/>
    <dbReference type="KEGG" id="tsr:106539646"/>
    <dbReference type="CTD" id="26576"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000293">
        <property type="entry name" value="SRSF protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF20">
        <property type="entry name" value="SRSF PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="77"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909947.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="34025" checksum="62BC2C41F234BAE6" modified="2020-10-07" version="1">MVLEVLGHQLLKWIIKSNYQGLPIPCVKSILCQVLQGLDYLHTKCKIIHTDIKPENILLCVDESYIRRLAAEATLWQQTGGPPPSGSAVSSAPQESLNGKLSKNKRKKLKRKQKRQSQLLAERLRDLQQLEDLAATNAGTSKSAETTEGHCSCPISVSGGGEPSGGSLPCSPQSLTSSSGFYTHNAYDSCNGHSPPGTASHHVSPDSATSGFSGSLFSGSALSGVSNQRERGGLLSPSTFGTSEFLVNPLEPQNADKIRVKIADLGNACWVHKHFTEDIQTRQYRALEVLIGAVYNTPADIWSTACMAFELATGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAM5</accession>
    <name>A0A6I9XAM5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prickle-like protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PRICKLE4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013911361.1">
        <property type="nucleotide sequence ID" value="XM_014055886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAM5"/>
    <dbReference type="GeneID" id="106540685"/>
    <dbReference type="KEGG" id="tsr:106540685"/>
    <dbReference type="CTD" id="29964"/>
    <dbReference type="OrthoDB" id="10069167at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09340">
        <property type="entry name" value="LIM1_Testin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09341">
        <property type="entry name" value="LIM2_Testin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000005">
        <property type="entry name" value="Testin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010442">
        <property type="entry name" value="PET_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047120">
        <property type="entry name" value="Pk/Esn/Tes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211:SF35">
        <property type="entry name" value="PRICKLE-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06297">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51303">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PET" evidence="8">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="130"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="195"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="337"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="487"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013911361.1"/>
        </source>
    </evidence>
    <sequence length="560" mass="62531" checksum="ED25606E7C8AFF75" modified="2020-10-07" version="1">MSQPSHIWSERERSPCSRIITSLTPASSSDSDSGCALEDYPGPTTEFPPSEPVLQSSSSFSSAVVPAAIHNQLRIKILLYQLPPQDCDERYCPFIGEEERKQLRTFAAWRRQESLGQGVLRHLPLTAQEYLCRNCDKNIIRGDQGVFASKLGEQCWWHPTCFVCHVCQQPLIDLIYFHRDNKIFCGRHHAEFFRPRCASCDQLIFTSECVEAEGMRWHEEHFCCLECDLPLRIQQYVMKGGQPCCCACFESLYADICQACGEIVGVNSKQIVLQGQHWHSKDSCFCCSFCRKALLSQPITIHNGLLFCSEACSLEKESALSSSGSDSSDSAFISAPSPDFTPISRARNSSLDCSSKTGRTQRDGREERAGAAVTERLHSCGNSSVYAEFDGQEESVSFRNRTITPQLHVFESLTVSPQEDVRISISHPMDTPWSTTVVRGNRNLKTYFQETSRPQADFHFKSRHSVASQASVLIMDFPDRIYEGSPGGKEVTMEDDDSKYLTCSSSSSSDSEPEGFFFGKPIPKPGSRYGAFANMEQETLGKGMGWAARARANSKHCSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAN0</accession>
    <name>A0A6I9XAN0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PDZ domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910852.1">
        <property type="nucleotide sequence ID" value="XM_014055377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAN0"/>
    <dbReference type="GeneID" id="106540302"/>
    <dbReference type="KEGG" id="tsr:106540302"/>
    <dbReference type="OrthoDB" id="42382at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11324">
        <property type="entry name" value="IL16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11324:SF16">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910852.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17142" checksum="3A73FA0E4EC136A6" modified="2020-10-07" version="1">MVVYDRYAPVRMYQYLPGAPDTVPMTHLTDADRSSDPAEEESSKENCQSPTHGGGILKYRSRSQGAATRLESVGEDDELTVENGDLNYEIPMKYSRGGRKHSLPQHLDTGSRQEYHIVKKSTRSLSAAQVEYPWRLTQPSIISNIVLMKGQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDR3</accession>
    <name>A0A6I9XDR3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M20 domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106539118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909278.1">
        <property type="nucleotide sequence ID" value="XM_014053803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDR3"/>
    <dbReference type="GeneID" id="106539118"/>
    <dbReference type="KEGG" id="tsr:106539118"/>
    <dbReference type="OrthoDB" id="6119954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052030">
        <property type="entry name" value="Peptidase_M20/M20A_hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575">
        <property type="entry name" value="PEPTIDASE M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575:SF0">
        <property type="entry name" value="XAA-ARG DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013909278.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17707" checksum="F9A1969B7B80BB9D" modified="2020-10-07" version="1">MCGRGYIRELNIFFDVPFNNPLFANPYNNIPPYRLFGIFQNIPPYRLFGIFICLGSTDFGNVSYVVPGIHSYFYIGSDALNHTEQYTTAAGSEEAQFYALRTAKALAMTALDVIFKPDVLEKIREDFRLTKLKEEGHLNVPGNAKGPDNSNGAECVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGX4</accession>
    <name>A0A6I9XGX4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Probable G-protein coupled receptor 153</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GPR153</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913366.1">
        <property type="nucleotide sequence ID" value="XM_014057891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGX4"/>
    <dbReference type="GeneID" id="106542224"/>
    <dbReference type="KEGG" id="tsr:106542224"/>
    <dbReference type="CTD" id="387509"/>
    <dbReference type="OrthoDB" id="9887972at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15907">
        <property type="entry name" value="7tmA_GPR153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022347">
        <property type="entry name" value="GCR_153/162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022335">
        <property type="entry name" value="GPR153"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16518">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 153, 162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16518:SF5">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 153-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01992">
        <property type="entry name" value="GPR153"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01991">
        <property type="entry name" value="GPR153GPR162"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="24"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="613"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013913366.1"/>
        </source>
    </evidence>
    <sequence length="654" mass="73393" checksum="3728FAAEFDEB976E" modified="2020-10-07" version="1">MNRERTLHDNAVAWLVCSGLAVLANAWGILSVSAKQKKWKPLEFLICTLAGTHILNIAVPITMYSVVQLRQQHSDYEWNEGLCKVFVSTFYTLTLATCFSVTSLSYHRMWMVRWPVNYRLSNTKKQAVHTVMGIWMVSFILSTLPAVGWHDTTERFYASDCRFIVTEIGLGFGVCFLLLISGSVAMGVVCVAIALFQTFSIQAGNNVDKNKFNVPTIVVEDAQGKRRSSIDGSEPVKTSLQITYLITGIVFLYDFLMGFPVLVVSFASLKSDVAYDWMVLCIVWCSVAQSLLLPLFLWACDRYRADVRSVWEKCVAIMSNDDGGEETSLERPIPAEATYSQPYPDNLSGDVLTVDQIASYDLSALERGIPQLYATKPVPEDRMQYLQVPPTRRFSHDETEIWTTSQISAYFQHWVAGEDITAELASLLLPQPRYDRRRTSVLSYQEERRPHRKRRKSAESPLSLKPFSCEDIHRQGGYKCFSKEDVMDFTDYPSPRRTPRRPPVRSASVSLLPDAFPRRSTAAVLRDFPLATFEREPQVLRSISVQGRGTSLAVPVSLPAQEDIRTLFPKGSLDMPCHEQIHVELCESPGMEDCPMIRSCSKPECFRAGLSPSWRGQDDLQKNGSKGSTSSFLSSPSASSGYVTFHSDSISSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH94</accession>
    <name>A0A6I9XH94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ERGIC1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ERGIC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913237.1">
        <property type="nucleotide sequence ID" value="XM_014057762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH94"/>
    <dbReference type="GeneID" id="106542115"/>
    <dbReference type="KEGG" id="tsr:106542115"/>
    <dbReference type="CTD" id="57222"/>
    <dbReference type="OrthoDB" id="270930at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF36">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="8">
        <location>
            <begin position="107"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913237.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="32765" checksum="9F86E921DA842BF8" modified="2020-10-07" version="1">MPFDFRRFDIYRKVPKDLTQPTFTGAIISVCCCFFILFLFLSELTGFIATEIVNELYVDDPDKDSGGKIEVNLNITLPSLHCELIGLDIQDEMGRHEVGHIDNSMKIPLNNGDGCRFEGHFSINKVPGNFHVSTHSATAQPQNPDMTHVIHKLSFGDKLQVHNIHGAFNALEGTNKLSSNPLASHDYILKIVPTVYEDMSGKQRYSYQYTVANKEYVAYSHTGRIIPAIWFRYDLSPITVKYTERRQPLYRFITTICAIIGGTFTVAGILDSCIFTASEAWKKIQLGKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI83</accession>
    <name>A0A6I9XI83_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenosine deaminase CECR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CECR1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913627.1">
        <property type="nucleotide sequence ID" value="XM_014058152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI83"/>
    <dbReference type="GeneID" id="106542424"/>
    <dbReference type="KEGG" id="tsr:106542424"/>
    <dbReference type="CTD" id="51816"/>
    <dbReference type="OrthoDB" id="7202371at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013659">
        <property type="entry name" value="A_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF39">
        <property type="entry name" value="ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08451">
        <property type="entry name" value="A_deaminase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenosine/AMP deaminase N-terminal" evidence="1">
        <location>
            <begin position="29"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913627.1"/>
        </source>
    </evidence>
    <sequence length="446" mass="51657" checksum="0FFE35D058C60D3A" modified="2020-10-07" version="1">MKVVVQSFGGWLILFFSCVSFCVTFQLHDREMLLEMEKIRRTGGNLTLRPEEEEFNSKLMKLKEAEIATAMETGLFPPAMHFFKAKELIEKSAVFDILKKMPKGGLLHIHDYAMVSPEWLIYNISYLPHCYVSFCKETVRFKFSKPSHPKKLYSCSKWILLETYRKNLKDVSAFDKRWVKTFLIVTDTPEETYPTQASIWKKFESIFKISSGLINYAPVFKSYLYQGLLELYKDNVQYVEIRVILPAIYEANGKVHGKVWSVKVFKKVAAKLKKKYPDFLGLKLIYTTTRYLSNEYLEICFFLLPTRTVGWEDGGKSLFELQKVLWLPKIKKITLPYFFHAGETGAQVLKLVSDLRNHPAAFLLSEGYPIVVSSDDPAIFGSKGLSHDFYEMFMGVGGMSANLRTIKQLVLNSFKYSSMKPNEKKKALGVWEKKWNDFIESFLHSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIN4</accession>
    <name>A0A6I9XIN4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA binding protein fox-1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106542526</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">RNA-binding protein that regulates alternative splicing events.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913782.1">
        <property type="nucleotide sequence ID" value="XM_014058307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIN4"/>
    <dbReference type="GeneID" id="106542526"/>
    <dbReference type="KEGG" id="tsr:106542526"/>
    <dbReference type="OrthoDB" id="5382468at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12407">
        <property type="entry name" value="RRM_FOX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000004">
        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025670">
        <property type="entry name" value="Fox-1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034237">
        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017325">
        <property type="entry name" value="RBFOX1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF25">
        <property type="entry name" value="RNA BINDING PROTEIN FOX-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12414">
        <property type="entry name" value="Fox-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037932">
        <property type="entry name" value="Ataxin_2_bd_A2BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="87"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037932-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913782.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="34551" checksum="5060E8A57122DCA2" modified="2020-10-07" version="1">MTQTFPAQYPPPQARLPPQYHLHTTPSEYPSPTALDHSLRIFPEQGTTASDPLPLPPPPAQAEEVPAPELSPETEDAESEENKAQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENSQDADRAREKLNSSIVEGRKIEVNNATARLVTKKPPVAPLVNGWKINPVVGAVYAPDLYTVASIPYPMMAPAALAYRGTLRGRGRATIYNPIRAAPAPTPVPAYGSVLYPDGLYSSDLYGYPAAYRVAQAPAAAAATAAAAYSDGYGRVYTTAEPYHHAVTPAYGVGAMASLYRGGCNRFTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLA9</accession>
    <name>A0A6I9XLA9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="12">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PIM3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013914761.1">
        <property type="nucleotide sequence ID" value="XM_014059286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLA9"/>
    <dbReference type="GeneID" id="106543308"/>
    <dbReference type="KEGG" id="tsr:106543308"/>
    <dbReference type="CTD" id="415116"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14102">
        <property type="entry name" value="STKc_PIM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000209">
        <property type="entry name" value="Serine/threonine-protein kinase pim-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000232">
        <property type="entry name" value="Serine/threonine-protein kinase pim-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017348">
        <property type="entry name" value="PIM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051138">
        <property type="entry name" value="PIM_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PIM-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037993">
        <property type="entry name" value="STPK_Pim-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="40"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="46"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037993-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037993-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013914761.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="36715" checksum="B12CD3B39515F4BC" modified="2020-10-07" version="1">MLLSKFGSLAHLCNPTSMDHLPVKILQPVKVEKEPFDKIYQVGSVLGSGGFGTVYAGNRIADGLPVAVKHVVKERVTEWGTIGGVMVPLEIVLLKKVGSGFRGVIKLLDWYERPDGYLIIMERPDLVKDLFDFITEKGALDEDTARGFFHQVLEAVRHCYSCGVVHRDIKDENLLVDLRTGELKLIDFGSGAILKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLYFRRRISLECQQLIKWCLSLRPSDRPTLEQIFDHPWLHKSEVAKSEDCDIRLRTIGTDVSRTSSSNESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLX8</accession>
    <name>A0A6I9XLX8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription initiation factor TFIID subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">TBP-associated factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106541039</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TAF3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911838.1">
        <property type="nucleotide sequence ID" value="XM_014056363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLX8"/>
    <dbReference type="GeneID" id="106541039"/>
    <dbReference type="KEGG" id="tsr:106541039"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15522">
        <property type="entry name" value="PHD_TAF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000317">
        <property type="entry name" value="transcription initiation factor TFIID subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46452">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46452:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="29"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911838.1"/>
        </source>
    </evidence>
    <sequence length="93" mass="10534" checksum="ABCE699534B74DF1" modified="2020-10-07" version="1">MGSSKVPVRSVVTETVSNYVIRDEWGNKIWICPGCEKPDDGSPMIGCDDCDDWYHWPCVGITAAPPEEIQWFCSRCASKKKDKKHKKGKHKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM51</accession>
    <name>A0A6I9XM51_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP-ribosylhydrolase ARH3</fullName>
            <ecNumber evidence="7">3.2.1.143</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ADP-ribose glycohydrolase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ADP-ribosylhydrolase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">O-acetyl-ADP-ribose deacetylase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Poly(ADP-ribose) glycohydrolase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">[Protein ADP-ribosylarginine] hydrolase-like protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">[Protein ADP-ribosylserine] hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">ADPRHL2</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>alpha-NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68792"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:77017"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="25">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="25">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013916954.1">
        <property type="nucleotide sequence ID" value="XM_014061479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM51"/>
    <dbReference type="GeneID" id="106545034"/>
    <dbReference type="KEGG" id="tsr:106545034"/>
    <dbReference type="CTD" id="54936"/>
    <dbReference type="OrthoDB" id="410104at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140290">
        <property type="term" value="P:peptidyl-serine ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4080.10:FF:000001">
        <property type="entry name" value="ADP-ribose glycohydrolase ARH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF24">
        <property type="entry name" value="ADP-RIBOSYLHYDROLASE ARH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 25" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="25">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013916954.1"/>
        </source>
    </evidence>
    <sequence length="375" mass="40869" checksum="05B7A4A81BE33FD3" modified="2020-10-07" version="1">MAAKVSQLTRSRCRGCLLGALLGDCLGGDFEARESVSAAELLRFVVALRPLQGPAAEPGEESGGGSSGGKLEGSRVSNILYYSDDTAMAHSIVRSLLAKQNFDEVDMAKRFAEEYKKDPDRSYGGGVVTVFKKLLSPKCRDVFEPARQQFNGKGSYGNGGAMRVAGISLAYSDVQDVKKFAKLSAELTHANSLGYNGAILQALAVHYALHGESNRDKFLDQLIGQMEDVEADDKSVADAQMLGYEDRPFSKRLKKIREFLEQGSVSRSDVLLELGNGIQALHSVPTAIYSFLRCMESHPDVPDSYNSLQRTIMYCILLGGDTDTIATMAGAIAGAFYGEEQVPQSWKESCEAYEETEKLADGLYELYHQPALSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPT2</accession>
    <name>A0A6I9XPT2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SREK1IP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SREK1IP1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Possible splicing regulator involved in the control of cellular survival.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915821.1">
        <property type="nucleotide sequence ID" value="XM_014060346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPT2"/>
    <dbReference type="GeneID" id="106544162"/>
    <dbReference type="KEGG" id="tsr:106544162"/>
    <dbReference type="CTD" id="285672"/>
    <dbReference type="OrthoDB" id="5596742at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31437:SF1">
        <property type="entry name" value="PROTEIN SREK1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31437">
        <property type="entry name" value="SREK1IP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13917">
        <property type="entry name" value="zf-CCHC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="109"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013915821.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17915" checksum="BB85333DCD8FD6CE" modified="2020-10-07" version="1">MALPGGNKDNIRAGCKKCGYPGHLTFECRNFLRVDPKRDIVLDVSSTSSEESEEEELGKLDAVPEKQGIPDEEEKKKIQKKSKEKSKSKKSRKRSHSSSITEDDEPKSKKQKSHKKEKKKVKKNKSKKRKHHHKKGGKKHKRGKDFSSSNSSDSSSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRV5</accession>
    <name>A0A6I9XRV5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cytochrome P450 2J2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106544661</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916486.1">
        <property type="nucleotide sequence ID" value="XM_014061011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRV5"/>
    <dbReference type="GeneID" id="106544661"/>
    <dbReference type="KEGG" id="tsr:106544661"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF134">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 2, SUBFAMILY AB, POLYPEPTIDE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013916486.1"/>
        </source>
    </evidence>
    <sequence length="303" mass="34834" checksum="5425DAACF7B639A6" modified="2020-10-07" version="1">MTRQAAEQWSDKGRISQEQPDDSIYEMFPFIMKYLPGSHQKALSAAEFIINFAKKEIVQHKENQNVHDPQDFIDFYLLQMEISKNDPDSTFDEDNLAHCIADLFIAGMETTNGTMQWALLLMANHLDIQDKVHKEIKDALSSSRSICYGDRKNLPYTNAVLHEIMRSKYIGLFGVPRQCTKDIYLSGFLIPKGAIILPELRSVLFDPEQWETPEKFNPNHFLDKEGHFVRKEAFMPFGAGARICLGELFTRIEFFIMFTSLLRKFRVQPPEGVEKLSEEPIIGVTVYPTPYKICVIPRSATSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS64</accession>
    <name>A0A6I9XS64_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">LIM/homeobox protein Lhx8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LHX8</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916902.1">
        <property type="nucleotide sequence ID" value="XM_014061427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XS64"/>
    <dbReference type="GeneID" id="106544993"/>
    <dbReference type="KEGG" id="tsr:106544993"/>
    <dbReference type="CTD" id="431707"/>
    <dbReference type="OrthoDB" id="125004at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021884">
        <property type="term" value="P:forebrain neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09381">
        <property type="entry name" value="LIM1_Lhx7_Lhx8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09383">
        <property type="entry name" value="LIM2_Lhx7_Lhx8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000050">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000023">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000031">
        <property type="entry name" value="LIM homeobox 6, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF117">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN LHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="127"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="216"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="218"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013916902.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="38521" checksum="A7A95A1841BE6908" modified="2020-10-07" version="1">MAEESERGAAAAAVTGRRRKSVGDTGLVSPEVVADEDSCSSSTPLSPSSSPRSLASGSGSCGSSKCICNSCGLEIVDKYLLKVNDMCWHVRCLSCSVCRTSLGRHTSCYIKDKDVFCKLDYFRRYGTRCSRCGRHIHSTDWVRRAKGNVYHLACFACFSCKRQLSTGEEFALVEEKVLCRVHYDCMLDNLKREVENGNGISVEGALLTEQDVNHPKPAKRARTSFTADQLQVMQAQFAQDNNPDAQTLQKLAERTGLSRRVIQVWFQNCRARHKKHVSPNHSSTAPVTTVQASRLSPPMLEEMAYSAYVPQDGTMLTALHSYMDAHSPSALGLQPLLTHSLTQLPINHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSH9</accession>
    <name>A0A6I9XSH9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DDX49</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013916701.1">
        <property type="nucleotide sequence ID" value="XM_014061226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSH9"/>
    <dbReference type="GeneID" id="106544830"/>
    <dbReference type="KEGG" id="tsr:106544830"/>
    <dbReference type="CTD" id="54555"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF25">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013916701.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="28772" checksum="F6610A45ACAE7197" modified="2020-10-07" version="1">MAGFRALGLDPWLASQAEQLGLSRPTPVQEACIPPALQEFLSHRSALCELKATSHKVAKKQRFAALAKFKSSVFKILIATDVAARGLDIPTVQVVINHNTPGLPKIYIHRVGRTARAGRHGIAITLVTQYDIHLIHAVEEQIKMKLQEFSTEEQKVLKILTQVNVVRRECEIRLEATDFDEKKEINKRKQMILEGKDPDLEMQRKAELAKIKKKKLKFQSQVHQTLQERQQKQLQQKLRKQARRKKQKQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSJ9</accession>
    <name>A0A6I9XSJ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Gastrula zinc finger protein XlCGF57.1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106544845</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916716.1">
        <property type="nucleotide sequence ID" value="XM_014061241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSJ9"/>
    <dbReference type="GeneID" id="106544845"/>
    <dbReference type="KEGG" id="tsr:106544845"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003288">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001343">
        <property type="entry name" value="Zinc finger protein 568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF377">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
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        <property type="match status" value="16"/>
    </dbReference>
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        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
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        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
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        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
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        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
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        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
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            <end position="513"/>
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            <end position="541"/>
        </location>
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        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013916716.1"/>
        </source>
    </evidence>
    <sequence length="582" mass="66773" checksum="733B8653C6F370AB" modified="2020-10-07" version="1">MKENGNLTGERNELFANLDIGSHEISVHPDLPKDGKNHEASSYVESWDETSDRNMHWKTHPGEKPFQCLECGKSFGRNSELIRHKRLHAGEKPYKCPECGKSFSQSRNLTSHQRSHTGEKPYKCLECGKCFSNSGILTSHQRSHTGEKPYQCSECGKCFSQSGILMAHQAMHTGQKPFPCLECGKRFRQLIHLTSHERIHTGEKPFQCTKCGKSFRQHIHLTSHQRTHTGEKPFRCQECGKTFSQSSHLIRHERTHTGEKPFKCLLCAKNFSHSSELVRHERTHTGEKPFKCPECDKSFSRSSELIRHKRIHTGEKPYKCLECDKSFVDSTNLIRHQISHTGEKPYKCPECGKCFSQSRSLTAHRRIHTGEKPFKCQECGKCFSQSGILAAHQGMHTGQKPFKCVECGKRFRQHIHLTSHRRIHTGEKPFKCPDCGRSFRQHIHLTSHKRIHTGEKPFQCQECGKSFSHNSILISHVRTHTGDKPYKCLECGKSFTWSNLLIRHERTHTGEKPFKCPECGKGFSERSLLSKHQRRHTGEKPFKCLECGKNFFSSTLLIRHQRTHTGGKPLNIWSVENPSGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUR4</accession>
    <name>A0A6I9XUR4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Netrin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106538458</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908420.1">
        <property type="nucleotide sequence ID" value="XM_014052945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUR4"/>
    <dbReference type="GeneID" id="106538458"/>
    <dbReference type="KEGG" id="tsr:106538458"/>
    <dbReference type="OrthoDB" id="9972745at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000081">
        <property type="entry name" value="Netrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000048">
        <property type="entry name" value="Netrin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF443">
        <property type="entry name" value="NETRIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 8" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026913648" evidence="9">
        <location>
            <begin position="18"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="11">
        <location>
            <begin position="32"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="10">
        <location>
            <begin position="375"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="408"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013908420.1"/>
        </source>
    </evidence>
    <sequence length="479" mass="53724" checksum="FBE9F6DFCCDDD967" modified="2020-10-07" version="1">MLHFPWGVAVFLPFLLGQTPDPHDPCYNANHRPQFCLPELSELASGRLVEASSTCNGPPGRFCAFRDSNDPASKFCQGCQEHTHRSERLTDGDGDTTCWWSQPTANATLSLALGRRMEILYVALRFCSPRPESLAIYKSMDYGRSWMPFQFFSSHCWRRYHLPPTTTIVKSMEHEAVCIEAQTALKPLTGGLVAFMPLAGRPSAQRFEYSPILHDWVTATDLRMSFDRMYSARILGMRRKEASYGVAELQVGGRCKCNGHASRCTAGKDGGVPQCDCRHNTAGPECDTCKTFYWDRPWQRATPKDAHECVACNCHRHSHRCRFSMELFELSGRRSGGICLNCRHHTAGRHCQYCSPGFKRDFSHPITSHRACKACQCHPIGATGAICNQTTGQCQCKNGVTGLICNRCAQGFQQSRSAHIPCIRIQEEVTTTVIPPQEWKAGEGVSGTECQKHCTPPQRQSVHMNLKNYCKKDYGTQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUY4</accession>
    <name>A0A6I9XUY4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphodiesterase</fullName>
            <ecNumber evidence="12">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106545759</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:16958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14653"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57867"/>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <dbReference type="EC" id="3.1.4.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:14654"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="12">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013917892.1">
        <property type="nucleotide sequence ID" value="XM_014062417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUY4"/>
    <dbReference type="GeneID" id="106545759"/>
    <dbReference type="KEGG" id="tsr:106545759"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013706">
        <property type="entry name" value="PDE1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08499">
        <property type="entry name" value="PDEase_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="2" id="KW-0140">cGMP</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="14">
        <location>
            <begin position="100"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="383"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="534"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="183"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013917892.1"/>
        </source>
    </evidence>
    <sequence length="691" mass="78467" checksum="8978503D5A5D0480" modified="2020-10-07" version="1">MAEDKREKIGIKTNSNQAKTKTCDQRTKKKLSATLLSLLDTEDELSSIRWDSVPMNVKEWLTGTFAKKKEAYRLQPDEITSIRSISYALENGVVVERQFRTATTSMGMAYELEVIEAIKTIDKWDFNVFTLDEAAEGHCLKYVMCEIFTKYELLKRFQIPFTILISFAKALEAGYNKHKNPYHNSLHAADVTQTVHALMIHTGMMQWFTDLEILAIFFSSIIHDYEHTGTTNHFHIETRSKAALLYNDRSVLENHHLSAAFRLLQDRDTNILDNLTKDEWREFRRMVINIVLSTDMSHHFQQMKVMKHILQCLQLADRTHKDQIMSFLVHMADINHPAKPWELHHRWSEALLEEFFEQGDKEAEMGLPISSLCDRKTTNIAESQIGEPLDKESNKAKKQVTVKSKGQKGKPQKTTKEKPQLSSPTDYCTAAQNPSTCRCQLCISKGCEPMDVSFIVYNANSSELSQPQQDTEVMDVTESYEELVDSESSWRKARSRNVGESMQEMRNMVPKTKVLRPDESDDVIIEEFIPNDPEPPKTTKPPDDITPKDFQQLPNLGILRTNLTKSDLEIFSLWNEEVMRREAEQRGKKLTDTTKQGAVQLIQNEQCRGSRKVSAQNPETFILQVISVDSPSATCQDHMGANCHPCQQGAASIPTNAPLPASNVSQANEANPCGAAAVSVTSLNYGVFKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVP9</accession>
    <name>A0A6I9XVP9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-related protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ACTR10</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Subunit of dynactin, a multiprotein complex part of a tripartite complex with dynein and a adapter, such as BICDL1, BICD2 or HOOK3. The dynactin complex is built around ACTR1A/ACTB filament and consists of an actin-related filament composed of a shoulder domain, a pointed end and a barbed end. Its length is defined by its flexible shoulder domain. The soulder is composed of 2 DCTN1 subunits, 4 DCTN2 and 2 DCTN3. The 4 DCNT2 (via N-terminus) bind the ACTR1A filament and act as molecular rulers to determine the length. The pointed end is important for binding dynein-dynactin cargo adapters. Consists of 4 subunits: ACTR10, DCNT4, DCTN5 and DCTN6. The barbed end is composed of a CAPZA1:CAPZB heterodimers, which binds ACTR1A/ACTB filament and dynactin and stabilizes dynactin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920234.1">
        <property type="nucleotide sequence ID" value="XM_014064759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVP9"/>
    <dbReference type="GeneID" id="106547542"/>
    <dbReference type="KEGG" id="tsr:106547542"/>
    <dbReference type="CTD" id="55860"/>
    <dbReference type="OrthoDB" id="337660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd10207">
        <property type="entry name" value="ASKHA_NBD_Arp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000762">
        <property type="entry name" value="Actin-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000075">
        <property type="entry name" value="Actin-related protein 10 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000017">
        <property type="entry name" value="Actin-related protein 10 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013920234.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="46251" checksum="75A70EE9D5FE21B1" modified="2020-10-07" version="1">MPLYEGLGSGGEKTAVVIDLGAAFTKCGFAGETGPRCVLPSEIKKPGVSKPIRIVQYNINTEELYSYLKEFIHMLYFRHLLVNPRDRRVVIVESVLCPSHFRETLTKVLFKYFEVPSVLFAPSHLMSLLTLGINSAMVLDCGYTESLVLPIYEGIPVLSCWGALPLGGKAIHKELEYQLLEQSTVDTGATKGQSLTSVMGSVPEDIVEDIKVRTCFVSDLQRGLKIQAAKFDMDDGAERPTPPPDVDYPLDGEKILHINGSIRDSVVEVLFVQDNEEKSVATLILDSLMECPIDTRKQLAENLIVIGGTAMLPGFLHRLMAEIRYLVEKPKYKEILASKTFRVHVPPAKPNCVAWLGGAIFGALQDILGSRSVSKEYYNQTGRIPDWCCLNNPPLEMMFDVGKTPPPLMKRAFSTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVS6</accession>
    <name>A0A6I9XVS6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein FAM189B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FAM189B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ENTREP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914748.1">
        <property type="nucleotide sequence ID" value="XM_014059273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVS6"/>
    <dbReference type="GeneID" id="106543298"/>
    <dbReference type="KEGG" id="tsr:106543298"/>
    <dbReference type="CTD" id="10712"/>
    <dbReference type="OrthoDB" id="10012909at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030431">
        <property type="entry name" value="ENTREP1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615:SF7">
        <property type="entry name" value="PROTEIN ENTREP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615">
        <property type="entry name" value="PROTEIN FAM189A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013914748.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="60706" checksum="A3120E36AEB91F7C" modified="2020-10-07" version="1">MPSRSDSSYSLTGRTSHSFTHLRVRRAWLQIILLLGFIQMILGVLIVTLSLLVTTSIPSQHPIGNSCPIWAGFPDLLFSICGLTIFSIVVCTLSAIMCCIQIFSVDILHVLSPPRSSSVTLDCTSPPDTYLQHMLDLDEFVPPVPPPPYYPPEYTCSSETDAQSITYNGSMDSPIPLYPTDFPPSYEAVMGLRGDSQVTLFDSQFTETSHGPCSCNQVPSVVLSGEAISVDSNSLIMSEIVDIPGDSSPSEDSCLLEGSGSACSVDYVLFRSIQRSRADYCLSVDCGHCGLHPQGPFEDIPPMRLRGERSFSCSTPGTVYDGLLEVNSAQTHSCHRLEGLGHNVGPCFPEVRMKPKMAVSARKEPRRPRRSSETSCQSPVIRCPLLRSHSDPGIGVSREAETDDQEVFCTKGSEDNASNSSAETGLCSEMCPSHPSLCDPLLLAHGAGKSKLELPAKVSQDLSKTITRSLGDLKACRGSRGLMARFLHRSKRNLTLPGVEMSSRGNKQVLRLGSPSNGIHLQSCGDLNSTSPLRRLLSGRRLDQRRPCSLSGLLRESNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWL1</accession>
    <name>A0A6I9XWL1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Inner centromere protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">INCENP</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INCENP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918562.1">
        <property type="nucleotide sequence ID" value="XM_014063087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWL1"/>
    <dbReference type="GeneID" id="106546252"/>
    <dbReference type="KEGG" id="tsr:106546252"/>
    <dbReference type="CTD" id="3619"/>
    <dbReference type="OrthoDB" id="6123at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990385">
        <property type="term" value="C:meiotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051257">
        <property type="term" value="P:meiotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051310">
        <property type="term" value="P:metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005635">
        <property type="entry name" value="Inner_centromere_prot_ARK-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13142">
        <property type="entry name" value="INNER CENTROMERE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13142:SF1">
        <property type="entry name" value="INNER CENTROMERE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03941">
        <property type="entry name" value="INCENP_ARK-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner centromere protein ARK-binding" evidence="9">
        <location>
            <begin position="739"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="256"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="369"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="519"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="664"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="714"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="438"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013918562.1"/>
        </source>
    </evidence>
    <sequence length="829" mass="96178" checksum="BFE09474D3C742FE" modified="2020-10-07" version="1">MPDLMALEEKTNKKLTEFIQNVHDRDFVWMDEILEEAIKMFKRSSFDGEPELMPKTPSQKNHSRKKRFSSIKNDQLATKRSSKRNSARIRASKRISHNAQHKKELEMQKAEVENTSPCVRVTRSKAARSLRSLSIAANPVIALAKGRIPLVEISANERRSAEVQVEKTPPQITKDALRHTLSPSPIKRAEPFSEKSVIIIQNTPEEKLKKEVGRTACKLKIATVSLYKTIEKEEPSSEKLCPQQQEDELLSELKGTLNLNESPHTPVPKGSRRSVRRSLMGRSSTSSRASLVNRYSLSTKRKQIVLRRSSRTVSKQQSIQQESVHTQKNSKEAVYKSNCYCYCYCYCYLSAELLSIAEEFLVKESTESRATLDSMSHSQKVDENLNKSLYSNKTGMQETPRSEEKQHKGESEDESSEMGDVLQKQPLSARRKPSYKRAIRDRDDGQHTEDELSPPRKKTLSPQCPASKVVRPLKTFLHTVQKNQMLMTPASANQKNTIKSFLRQNTPLRFTPKGGFVEKERQRLETMRKKEEAEQQRKRKMEEEKKRRLEEMKRKREERLRKVLQVRERVEQLEEEKKKRLEQKMAQHEGKNEKKKREEEQERARKIAEQHKAELEREKELSAKRELEKKQEQEKIQAQRKHEQQEKEKAARLQREVLQAAKEKERLKKEMEEKERKLQEQRKQEEELLKTTSEAQAKIANKQLNVTITIENSPNCDSYPMTPQGPKQPKIDLNNYGMDLNSDDSTDDESQPRKTIPTWASGSQLSQAILHQYFNPPDTKALFGVVKSPKLEEIFDKSKPRYFKRTSSAVWNSPPFQGGKTLSSAFKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXK9</accession>
    <name>A0A6I9XXK9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein CNPPD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CNPPD1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNPPD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918541.1">
        <property type="nucleotide sequence ID" value="XM_014063066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXK9"/>
    <dbReference type="GeneID" id="106546242"/>
    <dbReference type="KEGG" id="tsr:106546242"/>
    <dbReference type="CTD" id="27013"/>
    <dbReference type="OrthoDB" id="244495at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013918541.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="47960" checksum="FF801FF738C1D679" modified="2020-10-07" version="1">MDLDRLLLDEEGAFSLSGFQEFTFLPKHQQLSDRVRKRLYYGWDKDSALDNLSSPVADIAVELFQKAAPSPIRRLQKKYVSHVSREACISPCSMMLALVYIERLRHRNPEYLQQISSSDLFLISMMVASKYLYDEGEEEEVFNDEWGAAGKMDVQTMNMLEMNFLRAIDWSLFTDLKEFFEVLSWLEGRVAEKQGVRRGWFTYTDLCVLMEQTLWQQALGQFYQQVAKLACILGVVYLTGVAAVFASAAVIHHTVHVKRSGPTAVSSTRDALVSCLPLSSSAQNMDYKQEPLYCLSWPRVENQTFQGEKQQRGISGVSVTVLYLWGKVLKALSYSPARISSSLYPHCHQPTSCMTANHTACAHFAQQSTRFGLPCCSSSSCWDRPLHLSPNCKDWPEPLALQQCPWQASLELGRFKTFMFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXM5</accession>
    <name>A0A6I9XXM5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Histone acetyltransferase</fullName>
            <ecNumber evidence="3 19">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC106546251</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 19"/>
    <dbReference type="RefSeq" id="XP_013918561.1">
        <property type="nucleotide sequence ID" value="XM_014063086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXM5"/>
    <dbReference type="GeneID" id="106546251"/>
    <dbReference type="KEGG" id="tsr:106546251"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036409">
        <property type="term" value="C:histone H3-K14 acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036408">
        <property type="term" value="F:histone H3K14 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045935">
        <property type="term" value="P:positive regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000092">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="21">
        <location>
            <begin position="332"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="17">
        <location>
            <position position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013918561.1"/>
        </source>
    </evidence>
    <sequence length="594" mass="68636" checksum="45CFB96057789CD5" modified="2020-10-07" version="1">MSRRKRNAGSSSDGTEDSDFSTDPEHTDSSESDGTSRQSARVTRSSARLSQSSQDSSPVRNPPSFAVEEPVYSTRRITRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRDTKNAADHDESPPRTPTGNAPSSESDIDISSPNMSHDESIAKDMSMKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKHERHFSISGCPLYHNLSADECKVRAQSRDKQVEDRMLSHRQDDNNRHATRHQAPTERQLRYKEKVAEMRKKRNASLTKEQKEKYMEHRQIYGNTREPLLENLTSEYDLELFRRAQARASEDLEKLRLQGQITEGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGPISVFEVDGKKNKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKIMDPSCLKWSPPKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXQ3</accession>
    <name>A0A6I9XXQ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Protocadherin Fat 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">FAT2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918596.1">
        <property type="nucleotide sequence ID" value="XM_014063121.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546283"/>
    <dbReference type="KEGG" id="tsr:106546283"/>
    <dbReference type="CTD" id="2196"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000116">
        <property type="entry name" value="Dachsous cadherin-related 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000015">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000021">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000026">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000037">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000041">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000051">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000052">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000064">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000065">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000071">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000079">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000080">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000089">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000194">
        <property type="entry name" value="FAT atypical cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000197">
        <property type="entry name" value="FAT atypical cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000215">
        <property type="entry name" value="FAT atypical cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000053">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000058">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000059">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000061">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000084">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000057">
        <property type="entry name" value="protocadherin Fat 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000198">
        <property type="entry name" value="Protocadherin Fat 2"/>
        <property type="match status" value="1"/>
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        </source>
    </evidence>
    <sequence length="261" mass="31166" checksum="2D4BDA0D259F97E9" modified="2020-10-07" version="1">MDEDGEREARLRRRLEVIMTKYGLSGKKEGQQWETNHIKEGFAQDGLGDPRLEKLWNKARSSGKFSDEELEKLWREFQHHKEKIHEYNILLETMSRTEDVHKNTIDQPLEEESAAWKELLHRKHSELKEQLHSINQGFERLRKVSHQGYDSASEFEEPRVMDLWDLAQSTNFTEKELESLREELKHFEAKVEKHHHYQKQLEISHQKLKHVEGTGDKEHLSRNKEKYTMLEEKTKELGYKVKKHLQDLSGRISRGLQHNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y203</accession>
    <name>A0A6I9Y203_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106548210</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921022.1">
        <property type="nucleotide sequence ID" value="XM_014065547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y203"/>
    <dbReference type="GeneID" id="106548210"/>
    <dbReference type="KEGG" id="tsr:106548210"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000005">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="51"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921022.1"/>
        </source>
    </evidence>
    <sequence length="169" mass="18402" checksum="94BF4BDE2A4A748A" modified="2020-10-07" version="1">MLFHLTILFKKFHISPYFFCPNYSTMTGRNRDRTSPRSDANMAGGKAGKDSGKAKAKAVSRSQRAGLQFPVGRIHRHLKTRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y267</accession>
    <name>A0A6I9Y267_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106547862</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013920601.1">
        <property type="nucleotide sequence ID" value="XM_014065126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y267"/>
    <dbReference type="GeneID" id="106547862"/>
    <dbReference type="KEGG" id="tsr:106547862"/>
    <dbReference type="OrthoDB" id="8940716at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1390:FF:000001">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000110">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF46">
        <property type="entry name" value="LEUCINE RICH REPEAT-CONTAINING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Roc" evidence="13">
        <location>
            <begin position="180"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013920601.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="33746" checksum="1923C00CC7726CD0" modified="2020-10-07" version="1">MQDCLKLEILSAAANRIYTIKDLPCSITTLKLSQNAFFTVPEAILLLPHLRSVDLSSNRIVTLPGPAFWKSSNLRELIFNHNQISILDLNDKASSWSRLEKLHLSYNRLREIPPDIGFLENLTSFDVSYNPAIRCFPDEMGKLLNIWDLPLEGLHLNLDFKHVGDKARDIIRFLQQRLKKAVPYHRMKLMVVGNAGSGKTTLLQQLMKCRRPDVGPQKPTIGIDVNDWKIPRKGKIKKDTVLNVWDFAGQEEFYSAHPHFMTQRALYLAVYDLSKGESEVDAMKPWLFNIKVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2L5</accession>
    <name>A0A6I9Y2L5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Extracellular matrix protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ECM2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920801.1">
        <property type="nucleotide sequence ID" value="XM_014065326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2L5"/>
    <dbReference type="GeneID" id="106548019"/>
    <dbReference type="KEGG" id="tsr:106548019"/>
    <dbReference type="CTD" id="1842"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070052">
        <property type="term" value="F:collagen V binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010811">
        <property type="term" value="P:positive regulation of cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000772">
        <property type="entry name" value="Extracellular matrix protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000130">
        <property type="entry name" value="extracellular matrix protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000284">
        <property type="entry name" value="extracellular matrix protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043184">
        <property type="entry name" value="ECM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46544:SF1">
        <property type="entry name" value="EXTRACELLULAR MATRIX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46544">
        <property type="entry name" value="EXTRACELLULAR MATRIX PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026824724" evidence="4">
        <location>
            <begin position="21"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="5">
        <location>
            <begin position="102"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013920801.1"/>
        </source>
    </evidence>
    <sequence length="729" mass="83687" checksum="4BAF5AC0FE22C96B" modified="2020-10-07" version="1">MQKPVLVYCLLLVYLSVALVQNKPNEMPGRARRRIIYRRLRKIPLRNHSLNKQSQIRPPISRTPLMSIPLTNIDGDITGVFNSLTQLDGQESNYNVLPGKEGRCIANGMIMFDKAVWSPKQCVTCLCSKGKVFCDETSCNPLTCPKSTKPEGECCPVCLETVLPVSTISQGDTAEVSGDSPVPTNPSEPVLHDMQTQDPMQKKEGTLEKNIHVKNKGKMKKKRIKSHKPRRKHEHINDQREEEVETRSEEEKRRVYEEEELRLEKEIKKQEQEQVKEEEQEQEKEEEQEHEQEETEQEKEGSEEEEDILRGDVFRMPPHFPIPEPSTEIPPLPTGCSALENTVSCINAKLTHIPIIRDEELTAIELDGNDITEIPDGAFNGIPNLERIDLRKNKITSFGIGPQAFKNLKNLQRLYMDGNTLVSIPHGLPPSLEELKINENEIHVINEDSFQGLKKLLILELESNKLSESNVSPLAFQPLKSLSYLRLGRNQFRTIPQGLPDSIEELYLDNNQIEEISEICFNHTKSLNIIVLRHNKLEESRIAPLAWINHENLESIDLSYNKLYQVPSYLPKSLVHLVLIGNQIDRIPGFVFGHMKPGLEYLYLSFNKLDDNGIDVVSFYGAYHSLREMFLDHNQLKSVPLGIAELKTLNFLRLNNNKIRTVPSERICRSRLNYEEENDDSEEEEDYEDSQLEHLHLEHNFINTRNLSPYAFSCVRSYSSVILKPQNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y336</accession>
    <name>A0A6I9Y336_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Periplasmic beta-glucosidase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106545540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013917613.1">
        <property type="nucleotide sequence ID" value="XM_014062138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y336"/>
    <dbReference type="GeneID" id="106545540"/>
    <dbReference type="KEGG" id="tsr:106545540"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009044">
        <property type="term" value="F:xylan 1,4-beta-xylosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031222">
        <property type="term" value="P:arabinan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044993">
        <property type="entry name" value="BXL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721:SF42">
        <property type="entry name" value="FIBRONECTIN TYPE III-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721">
        <property type="entry name" value="SUGAR HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="3">
        <location>
            <begin position="166"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013917613.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="28729" checksum="18DF60EB6B4A2024" modified="2020-10-07" version="1">MESESNDRKDLSLPGHQLDLLQSSVAWATGRPVILLLFNAGPLDISWAKEQDGVGAIVACFFPAETTGLAVAKMLVGAEGANPAGRLPATWPAGMDQVPPIENYTMIGRTYRYYGHEAPMYPFGYGLSYTAFQYRDLVVSADQVPICGNLSISAVVENVGSRDGEEVVQLYLHWGQASVPMPRWQLVGFRRLPIRQGQAAKVAFLLSFSQRAVWDGQWWLEPGTFTVFVGGQQPFQEVRLPSNVLQTDFHVFGDSRSASQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y363</accession>
    <name>A0A6I9Y363_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Roundabout homolog 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ROBO4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013917663.1">
        <property type="nucleotide sequence ID" value="XM_014062188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y363"/>
    <dbReference type="GeneID" id="106545574"/>
    <dbReference type="KEGG" id="tsr:106545574"/>
    <dbReference type="CTD" id="54538"/>
    <dbReference type="OrthoDB" id="428111at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002530">
        <property type="entry name" value="CBN-SAX-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000840">
        <property type="entry name" value="Roundabout guidance receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000065">
        <property type="entry name" value="roundabout homolog 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF11">
        <property type="entry name" value="ROUNDABOUT HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="31"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="132"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="7">
        <location>
            <begin position="245"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="7">
        <location>
            <begin position="343"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="564"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="629"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="680"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="694"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917663.1"/>
        </source>
    </evidence>
    <sequence length="729" mass="80868" checksum="BC45FC2EF5B3D269" modified="2020-10-07" version="1">MVWGHDLGRQEEELQTRREAGSRSRSVEFAPQILDHPSNLVVRKDQPATLNCRADGNPAPTIEWYRNGKYVKPSKDDATSQPTLLPDGSLFFLRLSQEKGKSDEGIYHCLARNHLGKAMSKNASLYLEALEEEFRLHPSDLKVTNGNSWFLECVPPLGHPEPTISWKKNGVPLSAENGHYEFSKEKLLVAHAQKSDAGAYACLAVNQVGEQVSRAAMVTVFDPLDTRQKEWNRPKDVAKELMDIIVCLVNVTIVPSAPAAHLHWKVTPASQPIEAYKVFYHSLLPTKMHWAEWDVARDQQTIIPGLERGYKYEFKVRPYLGKIHGTDSNVRQLYIPEEVPSAAPQIISITVSRGGNSTVILHWEPPPPDAHNGIIKAYQVWFLGNETHPGFNRTVDGDTHSLETTGLPNGHKYCVKVAAINGAGVGVASHPVCGTADPIVEKMAKSSDFLSANYMLEVVRQPVFIASIGSALWIMLMVLAVYVCQQQARQFGCGRRYALGEGLYRNASDDTIIKHRIDSSDSPWLSNTWKSTSGSKNYSTSSSLSSQLLWMESKDSQEFHKSTVSFDRQSQGSRIQTAPLVPDSSNSSPYGAFRMNLPAKDLKAFYSSLPYLSNPNLKSMALTQAGELMSHGSQNLRTDRASGQGRTQQGPWKAAGSLSPNRTIRGSWVKYDKKELQQVHSTPMSPSGFSADWSNHSSPRSVVSSGKHQRVHGKRRNVCLKVWGKDSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y435</accession>
    <name>A0A6I9Y435_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ADP-ribose glycohydrolase OARD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">O-acetyl-ADP-ribose deacetylase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Terminal ADP-ribose protein glycohydrolase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">[Protein ADP-ribosylglutamate] hydrolase OARD1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16 17 18 19" type="primary">OARD1</name>
    </gene>
    <organism evidence="15 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">ADP-ribose glycohydrolase that hydrolyzes ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on glutamate and O-acetyl-ADP-D-ribose. Specifically acts as a glutamate mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to glutamate residues on proteins. Does not act on poly-ADP-ribosylated proteins: the poly-ADP-ribose chain of poly-ADP-ribosylated glutamate residues must by hydrolyzed into mono-ADP-ribosylated glutamate by PARG to become a substrate for OARD1. Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins. Catalyzes the deacylation of O-acetyl-ADP-ribose, O-propionyl-ADP-ribose and O-butyryl-ADP-ribose, yielding ADP-ribose plus acetate, propionate and butyrate, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68792"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:77017"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912042.1">
        <property type="nucleotide sequence ID" value="XM_014056567.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912043.1">
        <property type="nucleotide sequence ID" value="XM_014056568.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912044.1">
        <property type="nucleotide sequence ID" value="XM_014056569.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912045.1">
        <property type="nucleotide sequence ID" value="XM_014056570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y435"/>
    <dbReference type="GeneID" id="106541203"/>
    <dbReference type="KEGG" id="tsr:106541203"/>
    <dbReference type="CTD" id="221443"/>
    <dbReference type="OrthoDB" id="2155246at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02901">
        <property type="entry name" value="Macro_Poa1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.220.10:FF:000007">
        <property type="entry name" value="O-acetyl-ADP-ribose deacetylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050892">
        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="14">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013912042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013912043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013912044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013912045.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="17304" checksum="8023F1B0B99F3AD7" modified="2020-10-07" version="1">MASTRISGNEERIKYVQGDLFTCPETDSLAHCISEDCHMSAGIAAIFKKKFGGVQELLSQHKKTGDVAILKRETRYIYYLISKNKYFHKPTYDNLRKSLEAMKIHCLKNAVTHISMPKIGCGLDRLDWKKVSTMLEEVFEDTNIHITVYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5N9</accession>
    <name>A0A6I9Y5N9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Centlein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106547362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919973.1">
        <property type="nucleotide sequence ID" value="XM_014064498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5N9"/>
    <dbReference type="GeneID" id="106547362"/>
    <dbReference type="KEGG" id="tsr:106547362"/>
    <dbReference type="OrthoDB" id="10011458at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010457">
        <property type="term" value="P:centriole-centriole cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038810">
        <property type="entry name" value="CNTLN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18957">
        <property type="entry name" value="CENTLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18957:SF0">
        <property type="entry name" value="CENTLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919973.1"/>
        </source>
    </evidence>
    <sequence length="334" mass="39048" checksum="CEC1CBE510929468" modified="2020-10-07" version="1">MAPPTPVKTVDLEMKQLQCKLKNANNEITKHSSTIKSLKSEVQRKEEQMQELQEKISRMERDISMKRHLIEDWRLRMKTYQEKEKSFNETLQCLEEKAKALTEDCSNKKISIDSLKQRLNVATKEKTQYEQMYHRTKEDLEKKELNLSNLEAKIAETECTMTELETTASQQLHSLARQSEQAIEMVQKKLIMSNEKVEEFITFVKSLATELQQNVKEMRTRIRCTKKMQEDKKCGGSISKESVHRAQSLAASILNISKTDLDEILDVVNDEETEQMKMEYESDKEWLGYLQKLLEGQFPFASYLMDAVLEKLNEQKKLAEEYSILIKRATRKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6D9</accession>
    <name>A0A6I9Y6D9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ral guanine nucleotide dissociation stimulator-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106547842</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013920583.1">
        <property type="nucleotide sequence ID" value="XM_014065108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6D9"/>
    <dbReference type="GeneID" id="106547842"/>
    <dbReference type="KEGG" id="tsr:106547842"/>
    <dbReference type="OrthoDB" id="26687at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF220">
        <property type="entry name" value="RAL GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="26"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013920583.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="33839" checksum="AEF2B645BECFF4F4" modified="2020-10-07" version="1">MRPSQVITEAAGTGDCAEIPALISFSVDEVAEQLTLMDAHLFKAVLPFHCLGCVWSQRDKKENQHMAPSVRATVTQFNAVTSCVITSVLGDLAMHIPQRAHLLEKWIQIAGQCRALRNFSSLHAILSALQSNSIYRLKRTWAAVNRDTRSTFRKLSRIFSEDHNHLNCREILLQEGDTRGPCDGWRNCQTPVRTSLKTPTIPYLGIFLTDLIMLDTALPDFVEGQLINFEKRRKEAAILSCIRHLQQSCQGYNLRLNPSFHLAFHHQRQLTEDQSYYISCMIEPPADSCPRRSCRWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6G9</accession>
    <name>A0A6I9Y6G9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RNA-binding protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RBM20</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914020.1">
        <property type="nucleotide sequence ID" value="XM_014058545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6G9"/>
    <dbReference type="GeneID" id="106542731"/>
    <dbReference type="KEGG" id="tsr:106542731"/>
    <dbReference type="CTD" id="282996"/>
    <dbReference type="OrthoDB" id="8949749at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12685">
        <property type="entry name" value="RRM_RBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034790">
        <property type="entry name" value="RBM20_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="475"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="1073"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="263"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="582"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="746"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="906"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="991"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="329"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="594"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="753"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="778"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="915"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="928"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="991"/>
            <end position="1020"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013914020.1"/>
        </source>
    </evidence>
    <sequence length="1232" mass="139258" checksum="05B0F8DF3A04745A" modified="2020-10-07" version="1">MESHDILSSAAKLLDKNIFTVNTANPLLPSSASLQLAQLQAQLTLHRLKLAQTAVNNNTAAATVLNQVLSKVAMSQPLFNQLRHPSMFNTLPGHAVGPGATNTRFPPGGIHFPTQNPPLAAHGGTLGSVNNLPNQNVSTVVLNPFGGVISQTTGQQAVSMGLNKTSASSTTGGFYDYNKQLFPSDAGQCCQQSFVTSGSHPVPVSSGNGMSEGQLGFQKEFYGAVSKGQHTTCTQSLTFAADSQNSHQNQKEKIGSIVHERDTSNQWENPSTFSNQNKTDIVPNTSMWPSTNVPYEVRSDLYNPEEPTPDTKFSTGTPHFFSRTKNDKQNYSNSQTRQKQESVTSGLPVRPLQSHGLNDFNSIVPLSFPHICTLCSKKVFDLKDWDQHIKGNLHIQNCLTFSENTDIHCVLHSAEGVLHSSSPNNKSVFNLSGTEDYPLNAESYNSASSFGQSSSTFSYLPPGGNVLQRKYVPGRVVHICNLPEGSCTENDVINLGLPFGKVTNYILMKSTNQAFLEMAYIEAAQAMVEYYKEKPAMINDDKLLIRMSKRYKELQLKKPGKNVAAIIQNIHSQRERDMLREADRYGQERPRSRSPINCSLSPRSQTPSFTSCSSPHSPLSTNRTEWGNGREPRDQFPYSQREEDKEVLLWRENVEEKRDWSDMWTHERKHHLRHLDLKEHVEATRGHKEKYLRGGSPNAKGAYLLPAYKMREEDCYRKSSKQKSYKYQNQTLDVALKGKRKEEIRLREHKHSHCEDTAKEERAEQKSIMMPDSIGQKQSDKEKLKDNAKKADDMTPGIVERNNVKKRKENGTENENWESGSDMEEETWYPTNMEELVTVDEVGEDDLIMEPDITELEEIVNVGQEEEECSIRTALAAGLEVKNEHSLLSTKDDKSNILETTIEAEKENADVSGSEGDNTVTKATHLNFDTEDKPDEFHENRSEKDSDDRKIDESSNIQLKLEKQYISSDPPKEKPFQQTDTLVNDYKEIETLENRNNGDKDILAKKSQEETNSGSEEKPKTQTNCRYTEIKTPDFPEVESKKSYFSPSWEQDDVFMELNIPLGVEFVVPRTGYYCKLCGLFYTNEDAAKVHHCRSAVHYKNLQLPVYMTDMKDIYIHIMNQDRLSPVPSFITREYLFQFKMLVFDLSTPAKEQDYLTLYLGILAYSSNICLHPPESAGLCDYFKLAVDRIEFFPRELAPLHLNPLFITFHKHKAVLHPDIQFLPQIVPFSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6Q8</accession>
    <name>A0A6I9Y6Q8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ADP-ribosylation factor-like protein 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ARL10</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920918.1">
        <property type="nucleotide sequence ID" value="XM_014065443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6Q8"/>
    <dbReference type="GeneID" id="106548119"/>
    <dbReference type="KEGG" id="tsr:106548119"/>
    <dbReference type="CTD" id="285598"/>
    <dbReference type="OrthoDB" id="8935954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053254">
        <property type="entry name" value="Arf-like_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46724:SF1">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR LIKE GTPASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46724">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="79"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="180"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013920918.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="27247" checksum="FA69A4D6458B2EA4" modified="2020-10-07" version="1">MAAAVAAFRHLSLALSAAVAALGSLLFIAWKVYFRDADDSCVGWRSRWERQLEAELRACNVRKEAEQPHYCEYQVLMLGLDGAGKSTILHYVCSPEAKKHIAPTQGFNSVQLHASGLQMDLLEVGGSQNLRFYWNQYLSKAHILVFVVDSADGPRLHIARQELHCLLAEDPQLPLIVLANKQDKNGALSVAELQEELALHNLDSQRKFFLLPTSATYAGLATANSVLQLKHLLVKILGRSSKMDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7M7</accession>
    <name>A0A6I9Y7M7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DnaJ homolog subfamily C member 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106543867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915445.1">
        <property type="nucleotide sequence ID" value="XM_014059970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7M7"/>
    <dbReference type="GeneID" id="106543867"/>
    <dbReference type="KEGG" id="tsr:106543867"/>
    <dbReference type="OrthoDB" id="69656at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000641">
        <property type="term" value="P:regulation of early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044978">
        <property type="entry name" value="GRV2/DNAJC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045802">
        <property type="entry name" value="GRV2/DNAJC13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19432">
        <property type="entry name" value="RME-8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaJ homologue subfamily C GRV2/DNAJC13 N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="GYF" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915445.1"/>
        </source>
    </evidence>
    <sequence length="1144" mass="131527" checksum="DA519DC4B87E5C2B" modified="2020-10-07" version="1">MNIIRENKDLACFYTTKHSWRGRYKRVFSVGTHAITTYNPNTLEVTNQWPYGDICSISPVGKGQGTEFSLTFRKGSGKKSETLKFSTEHRAELLTEALRFRTDFAEGKITGRRYNSYKHHWSDSRKPVILEVTPGSFDQINPTTNKILCSYDYRYIEGFVDLSDYPGGFCIIYGGFSRLHLFASEQREDIIKSAIEHAGNYIGISLRTRKEPLEFEQYLSLRFGKYSSDEYITSLAEFVVQKISPRHAEPVKRILALTETCLVERDPASYNIATLKPLGEVFALVCDSENPQLFTIEFIKGQIRKYSSTERDSLLASLLDGVRASGNRDVCVKMTPTEKGQRWGLLSMPVDEEVESLHLRFLAAPPNGNFADAVFRFNSNISYSGVLHAVTQDGLFSENKEKLINNAITALLSQEGDITASIAELESQFQAVRRLVASKAGFLAFTQLPKFRERLGVKVVKALKRNNDGVTHASIDMLCALMCPMHDDYDLRQEQLNKASLLSSKKFLENLLEKFNSHVEYGTGALVISSLLDFLTFALCAPYSETTEGQQFDMLLEMVASNGRTLFKLFQHPSMAIVKGAGLVMKAIIEEGDKEIATKMQELALSEGALPRHLHTAMFTISTDQRMRTNRQLSRHLVGLWTAENTTALNLLKRILPSGLLAYLDSNDPVPEKDWDRMHIRDNLKIANDQYGKFNKVPEWQRLAGKAAKGVEKFAKEKVDLVLMHWRDKMGIAQKEDRNNMNLNQKPVILRKRRQRIKIQGNWDLFYYRFLQDHARSNLIWNFKTREELRDSLETEMRAFQIDRELGSTNVISWNHHEFEVKYECLSEEIKIGDYYLRLLLEEDESEESEAIKKSYEFFNELYHRFLLTPKINMKCLCLQALAIVYGRCYEEIGPFGDTKYIVGMLERCTDRLERDRLIIFLNKLILNKKNVKELMDSNGIRILVDLLTLAHLHTSRATVPFQSNVIEAAPDMKRESEKEWYYGNAEKERRGPYSFNEMQELWTEGKLTSKTRCWAQGMDGWRPLQVIPQLKWCLLATGQAVLNETDLATLILNMLVTMCNYFPSRDQDNAIIRPLPKVKRLLSDNTCLPHIIQLLLTFDPILVEKVAILLFQITHHSFWEDMPQNASLNYILTFCLFRFESLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8E9</accession>
    <name>A0A6I9Y8E9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">T-cell surface protein tactile</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CD96</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916805.1">
        <property type="nucleotide sequence ID" value="XM_014061330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8E9"/>
    <dbReference type="GeneID" id="106544924"/>
    <dbReference type="KEGG" id="tsr:106544924"/>
    <dbReference type="CTD" id="10225"/>
    <dbReference type="OrthoDB" id="9904226at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042381">
        <property type="entry name" value="CD96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15317">
        <property type="entry name" value="T-CELL SURFACE PROTEIN TACTILE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15317:SF1">
        <property type="entry name" value="T-CELL SURFACE PROTEIN TACTILE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916805.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="34232" checksum="65AF13D97EC12CC1" modified="2020-10-07" version="1">MWYMGGKILNEKSEGLLIENKNINQVGSFYEKTSFLSLRNITHSTFNQPFKCMSAYYFTRNDRWKFSSKEILIPYGVQNTSLAYSEFISHITAISDSTTERLQPTISEASGITLVPDSTSEKHLHSTKTLQSDTTTKLFSDFTVPQKILHSTKTLQSSTTTMLLRDSTVPQKSLQPRQNDTVITLFGETMVPQRNLSIRRVTELTTMNSPAHSYTVKDGLNTSSAIIPKHSTFPWPVVSFLLFFCTCLIICGIRKWCQHQREIMNKPPSFKPPPPPVKYSSIQVYNEIYSSSDELENLKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA04</accession>
    <name>A0A6I9YA04_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein lin-28 homolog A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LIN28A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the lin-28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919580.1">
        <property type="nucleotide sequence ID" value="XM_014064105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA04"/>
    <dbReference type="GeneID" id="106547070"/>
    <dbReference type="KEGG" id="tsr:106547070"/>
    <dbReference type="CTD" id="79727"/>
    <dbReference type="OrthoDB" id="422005at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000007">
        <property type="entry name" value="Protein lin-28 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000087">
        <property type="entry name" value="Protein lin-28 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051373">
        <property type="entry name" value="Lin-28_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054081">
        <property type="entry name" value="Lin-28A-like_Znf-CCHC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109">
        <property type="entry name" value="PROTEIN LIN-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109:SF2">
        <property type="entry name" value="PROTEIN LIN-28 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21890">
        <property type="entry name" value="Lin-28A-like_zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CSD" evidence="19">
        <location>
            <begin position="37"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="18">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="175"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013919580.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="22733" checksum="C4716CFEA172A21D" modified="2020-10-07" version="1">MGSVSNQQFAGGCAKAGEEEPSGDSPRPDEDSQPLLHGAGICKWFNVRMGFGFLSMTTKEGVALESPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSSKGLESIRVTGPGGVFCIGSERRPKGKNLQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSIAHMVANCPIKTQQSPSSQGKPTYFREEEEMHSSPFLPETRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCQ8</accession>
    <name>A0A6I9YCQ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IQ and ubiquitin-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106548976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921951.1">
        <property type="nucleotide sequence ID" value="XM_014066476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCQ8"/>
    <dbReference type="GeneID" id="106548976"/>
    <dbReference type="KEGG" id="tsr:106548976"/>
    <dbReference type="OrthoDB" id="10265862at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037695">
        <property type="entry name" value="IQUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21074">
        <property type="entry name" value="IQ AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21074:SF0">
        <property type="entry name" value="IQ AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013921951.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="18682" checksum="14E1BE4DDF0F58BF" modified="2020-10-07" version="1">MSVNRIILISVSHLIFDALGEFEKIHFYSFFFPPLVFILQLQDLHYMVEHFWDSQSALSAWDDLYDLVMVRWNKWQEWSPWNTILLKEDEAEAHLKLSDLEKAYEKVFIHRVRHKQMLARNYFSQFPELAATLHKSDKQTNTEELSVMKSVTTASSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDR1</accession>
    <name>A0A6I9YDR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106549224</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922292.1">
        <property type="nucleotide sequence ID" value="XM_014066817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDR1"/>
    <dbReference type="GeneID" id="106549224"/>
    <dbReference type="KEGG" id="tsr:106549224"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF38">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013922292.1"/>
        </source>
    </evidence>
    <sequence length="309" mass="34689" checksum="F4219E34E96732BB" modified="2020-10-07" version="1">MESKQPLPTEPPPDYSDQGYAVPGYPGTQVQVGIPQDASGLLQYRSQSTLIVQPNALSYGALQALSAPTTAEPVVWMPAPPVPPKCPPGLEYLSQMDQVIVDQQPELMEMISGYESCNKYEVKNPTGQWIYFAVEENDDYNLYRYGTARSFIIKLFDSTNQPVMQLSRNFHCICCCFPCICCLQKLEVQAPLGTVIGYVKQKYYVCTPRFVIQNEAGENIVKLVGPNLCNVRFRDVPFEIIPLNQKSAIGKIIRKWKGFAIETTSGASTFITQFPMDLEVKTKALLLGACFLLDFMFNERTGPPRRDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDR6</accession>
    <name>A0A6I9YDR6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane and coiled-coil domain-containing protein 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMCO6</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922184.1">
        <property type="nucleotide sequence ID" value="XM_014066709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDR6"/>
    <dbReference type="GeneID" id="106549144"/>
    <dbReference type="KEGG" id="tsr:106549144"/>
    <dbReference type="CTD" id="55374"/>
    <dbReference type="OrthoDB" id="21522at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16356:SF1">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16356">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 6 TMCO6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013922184.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="58391" checksum="CE743ACCF7E29AA8" modified="2020-10-07" version="1">MMAASVPGTAGPKSWKRSCYRSHGSSVEHLRTWRREREAALRSARRVKQLFSKRRLCEDKSSDGKVEMDNETAALLSEEEVSQLLKKIQRGSSEDRTDFMQLLRQGLQYKEIQQKFIRLEGNMSVLIGLFTSNLANLQMDAACCLHELSLSTDPAVVEACLPATSYLLTYLSGHSVALMELCLYTLGNLVVEMKAVKKHLLPQGIISVLASCIQSPHVMIQEGVGYVLSQLLQSKEAAAEIIPLLLESTLPQHMLHLVCSRLEDGIGTAVECAWGLHYIICSQVNNSLLISQRTMPSLVQLLLELSSQIATTSVEGLELLICPVVRCVGNLLAEDKANNDELQIKEECLLKALFVFMQFFLPKHTFVVSECLWLLNNLTVQSTTSCSALLSLEVFSDLLKLLRSYQVISLLVLTVLCNIAAKGAAYCEMLHQKAVLPSLVNILVFSDVQVVGQNLELLHLIFLYWPKAAIDFVNHSGLHALQQHQNNLHLQEQVKVLIHTVSQITGVPQNNPLGTENLHFTSLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE54</accession>
    <name>A0A6I9YE54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Voltage-gated potassium channel subunit beta-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">K(+) channel subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Kv-beta-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">KCNAB2</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922453.1">
        <property type="nucleotide sequence ID" value="XM_014066978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE54"/>
    <dbReference type="GeneID" id="106549355"/>
    <dbReference type="KEGG" id="tsr:106549355"/>
    <dbReference type="CTD" id="8514"/>
    <dbReference type="OrthoDB" id="1720422at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098900">
        <property type="term" value="P:regulation of action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19141">
        <property type="entry name" value="Aldo_ket_red_shaker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000001">
        <property type="entry name" value="voltage-gated potassium channel subunit beta-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005401">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF1">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01579">
        <property type="entry name" value="KCNAB2CHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="24" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="22">
        <location>
            <begin position="52"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013922453.1"/>
        </source>
    </evidence>
    <sequence length="367" mass="41023" checksum="66D0E61EE0EB283F" modified="2020-10-07" version="1">MYPESTTDSPARFSNRHTGSPGMIYSARYGSPKRQLQFYRNLGKSGLRVSCLGLGTWVTFGGQITDEVAEQLMTLAYDNGINLFDTAEVYAAGKAEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKGSLERLQLNYVDVVFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDGGIPPYSRASLKGYQWLKDKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNESVSSVLLGASNADQLMENIGAIQVLPKLSSSIIHEIDSILGNKPYSKKDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF65</accession>
    <name>A0A6I9YF65_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted frizzled-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Frizzled-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">FrzB-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">FRZB</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secreted frizzled-related protein (sFRP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922853.1">
        <property type="nucleotide sequence ID" value="XM_014067378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF65"/>
    <dbReference type="GeneID" id="106549671"/>
    <dbReference type="KEGG" id="tsr:106549671"/>
    <dbReference type="CTD" id="2487"/>
    <dbReference type="OrthoDB" id="5946121at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07441">
        <property type="entry name" value="CRD_SFRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03581">
        <property type="entry name" value="NTR_Sfrp3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000010">
        <property type="entry name" value="secreted frizzled-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000005">
        <property type="entry name" value="secreted frizzled-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035813">
        <property type="entry name" value="NTR_Sfrp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041759">
        <property type="entry name" value="SFRP3_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF97">
        <property type="entry name" value="SECRETED FRIZZLED-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026943722" description="Secreted frizzled-related protein 3" evidence="16">
        <location>
            <begin position="41"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="17">
        <location>
            <begin position="38"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="18">
        <location>
            <begin position="186"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="43"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="51"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013922853.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="36916" checksum="1FB6C0680136A0D1" modified="2020-10-07" version="1">MGEQRMMPLGCGKMLLLLPPSTLLLALAGLLASWAPLARGAACEPVRIPLCKSLPWNMTKMPNHLHHSTQANAILAMEQFEGLLGIHCSPDLLFFLCAMYAPICTLDFQHEPIKPCKSVCERARAGCEPVLVKYGHAWPDSLACDELPVYDRGVCISPEAIVTAEGSDFPMDSNNGNCRGTNAERCKCKPIKATQKTYLRNNYNYVIRAKVKEVKTKCHDVTAVVEVKEILKSSLVNIPRDTVNLYTNSGCLCPPLNANEEYIIMGYEDEERSRLLLVEGSIAEKWKDRLGKKVKRWDQKLRHPAKGRNEPGQSDSAPKSGRSTNPRQRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFU4</accession>
    <name>A0A6I9YFU4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106549901</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923133.1">
        <property type="nucleotide sequence ID" value="XM_014067658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFU4"/>
    <dbReference type="GeneID" id="106549901"/>
    <dbReference type="KEGG" id="tsr:106549901"/>
    <dbReference type="OrthoDB" id="9049408at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="58"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923133.1"/>
        </source>
    </evidence>
    <sequence length="145" mass="16111" checksum="53CA084A9D9B3262" modified="2020-10-07" version="1">MYDRRLYCHLQTPVGGGKTDKYQTILLDSPAIQLEVCNTLNPDTLLPQVDTEFDQPLPNADCEWFTDGSGFMMEGLRKAGYAVVIVWEVIEAKPLPVNTSAQLAELVALTRALQLAAGLTVNTQIANMPLEFCTPTELYGRKEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHZ3</accession>
    <name>A0A6I9YHZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Steroidogenic factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Nuclear receptor subfamily 5 group A member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">NR5A1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR5 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923811.1">
        <property type="nucleotide sequence ID" value="XM_014068336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHZ3"/>
    <dbReference type="GeneID" id="106550430"/>
    <dbReference type="KEGG" id="tsr:106550430"/>
    <dbReference type="CTD" id="2516"/>
    <dbReference type="OrthoDB" id="5984981at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07070">
        <property type="entry name" value="NR_LBD_SF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000011">
        <property type="entry name" value="Nuclear receptor subfamily 5, group A, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF24">
        <property type="entry name" value="STEROIDOGENIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002530">
        <property type="entry name" value="Nuc_orph_FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="12 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 20" id="KW-0804">Transcription</keyword>
    <keyword evidence="10 20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="22">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="23">
        <location>
            <begin position="260"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="119"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR002530-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013923811.1"/>
        </source>
    </evidence>
    <sequence length="502" mass="56188" checksum="975DE694D258F0DF" modified="2020-10-07" version="1">MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQNCKIDKTQRKRCPYCRFQKCLAVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALFRANGFKLETVPPPGGPPPPPPPPPPPPIVSPVQAADYGGLPPSLHGLAHHQHPGGLGKALPPPSAAAMTPIDYERSPYGTPGLAAPGPVGPGPLSGYHYPPFASRAIKSEFPEPYAAHPYEAAPAYPFPEPYPGVLGGGGSPAAGLDVPEVILKLLQLEPEEPQLKTRILNCLQQEQQQSKSRQEKLTTFGLLCKMADQTLFAIVEWARSCIFFKELEVGDQMKLLQNCWSELLVFDHIFRQVQYGKEHSLVLVTGQEVDMSTIATQAGSIVNNLVLRAQELVLNLHSLQIDRQEFVCLKFLILFSLDVKYMENSSLVKEVQDKANTALMEYTLCHYPHTMDKFRHLLMQLEDIRALSMQAEEYLYHKHLGGEVPCNNLLIEMLHAKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI18</accession>
    <name>A0A6I9YI18_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Klotho isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KL</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923604.1">
        <property type="nucleotide sequence ID" value="XM_014068129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI18"/>
    <dbReference type="GeneID" id="106550258"/>
    <dbReference type="KEGG" id="tsr:106550258"/>
    <dbReference type="CTD" id="9365"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000042">
        <property type="entry name" value="Klotho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000062">
        <property type="entry name" value="Klotho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF10">
        <property type="entry name" value="KLOTHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026695244" evidence="10">
        <location>
            <begin position="25"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="965"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923604.1"/>
        </source>
    </evidence>
    <sequence length="1015" mass="117251" checksum="F35A9F2AD84B3381" modified="2020-10-07" version="1">MIPAATSGLPFLVLGMFLCRPLAGKPGQGEATWSRFARLPFPDDALFLYDTFPRGFLWGAGSAAYQVEGAWNRDGKGPSVLDAFTHRSAAALFRNPAELPSWDSASGSYDHVERDLDGLSALAVSHYRFSLSWARLLPNGTAPANLAGLRYYARLLSGLRQRGIEPVVTLYHWDLPRTLQDLYGGWENPIVVELFLDYAQFCFQHFGRQVRFWLTIDNPYLVAWHGYATGRLAPGVRGGRKMGYKVAHNLLKAHAKVWHLYNDNFRPTQKGQVSIALSSHWIKPQNMTKKNIKNCQKSLDFVLGWFAKPIFTDGDYPQSMKRYLSLTLPEFTDAEKNLIRGTADFFALSFGATLSFSRVDPDMLFQQQQTLNLRQLLYWISREYNPSQIFIVENGWYVSGTTTTDDANYMYYLKNFILDTLKAIRYDGVAVMGYTVWSLMDGFEWFRGYVRRGLFYVDFQSQDKKMIMKSSGLFYQKLIQDNGFLPTPQTQPFKGTFPCNFAWGVTENFLQIDAKRSQFTNPNIYIWGLHQTKNLLKIDGSGVLKLKPHCVDFSHVRPQISLLQKMHITHFHFSLAWSLILPLGNETNVNQTLLFYYQCIFNELIKVNITPVVALWQPEATNQGLPFSLANNGGWESQHTVEAFVEYAQFCFKNFGHQVKFWITMNEPNVENLTYKAAHNLLKAHAKAWHLYDKKFRKIQNGKISISLHADWVEPASPFLKKGKEAARRALEFDIGWLAEPIFGSGNYPEVMRELLRQKNDLGFHSSQLPYFSAEERNLIHGSFDFFALSHYTTTLVSSEKDIPKKYDPLLDIQTVKDLTWLESPSQTPVVPWGLRKLLNWVKHKYGNIPIYILASGIDDDRDQDYDKLRIHYLENYINEALKAYTLDDVNLRGYFVYSFSDRGNSRSYGLYRYRMNKYEAKPSLMYYRQIIDNNISPGSESQNQLCPKELLRCLECESLQRRRFLLVFISFILFAFIVIIFLMAYYSKKTERRSKYPYPVLCRLSPVLLGKIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIJ5</accession>
    <name>A0A6I9YIJ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GPX4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(13S)-hydroperoxy-(9Z,11E)-octadecadienoate + 2 glutathione = (13S)-hydroxy-(9Z,11E)-octadecadienoate + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:48888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57466"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:90850"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:48889"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a hydroperoxy polyunsaturated fatty acid + 2 glutathione = a hydroxy polyunsaturated fatty acid + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:19057"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:131871"/>
            <dbReference type="ChEBI" id="CHEBI:134019"/>
            <dbReference type="EC" id="1.11.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19058"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Has a tendency to form higher mass oligomers. Interacts with FUNDC1; this interaction promotes GPX4 recruitment into mitochondria through TOM/TIM complex where it is degraded by mitophagy.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924193.1">
        <property type="nucleotide sequence ID" value="XM_014068718.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550757"/>
    <dbReference type="KEGG" id="tsr:106550757"/>
    <dbReference type="CTD" id="2879"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047066">
        <property type="term" value="F:phospholipid-hydroperoxide glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF134">
        <property type="entry name" value="PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8 10" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0712">Selenocysteine</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924193.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13542" checksum="699D6062E8AC371D" modified="2020-10-07" version="1">MYDFHALDIDGNDVSLERYRGTVCIITNVASQXGKTDVNYTQFVQLYSRYAEKGLRILAFPCNQFGKQEPGTEEEIKAFAESYGVKFDMFSKVEVNGDNAHPLWKWLKNQPKGRGTLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJB3</accession>
    <name>A0A6I9YJB3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N(G),N(G)-dimethylarginine dimethylaminohydrolase 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550646</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDAH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924059.1">
        <property type="nucleotide sequence ID" value="XM_014068584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJB3"/>
    <dbReference type="GeneID" id="106550646"/>
    <dbReference type="KEGG" id="tsr:106550646"/>
    <dbReference type="OrthoDB" id="10016839at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000004">
        <property type="entry name" value="N(G),N(G)-dimethylarginine dimethylaminohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033199">
        <property type="entry name" value="DDAH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737">
        <property type="entry name" value="DIMETHYLARGININE DIMETHYLAMINOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737:SF16">
        <property type="entry name" value="N(G),N(G)-DIMETHYLARGININE DIMETHYLAMINOHYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924059.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="31033" checksum="D848C7992322192A" modified="2020-10-07" version="1">MASVSSFGKYTHAIVRCIPSSFATAETTADGSDSGADPVDLAKAHRQYGVYTGILRQKLGLQVIELAADEGFPYATLVGDLAVIQGDTVLLTRPWMPTRRDEISCVKKVLEELKLRVVEVTDEGATLDGSDVLFTGREFFVGISKWTNHRGAEMVADAFRDFAVSTVPVSGTLHLRSFCSMAGPDTVVIGSSEVAKKAMKTMEQLTDHHYETLTVPDDPAANCIYARLGQKSSVLLHRSTEEYPNSVQPFQKLPDYTLIPAACTEVAKLGGTLTSCSLLINKKLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJQ8</accession>
    <name>A0A6I9YJQ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein NipSnap homolog 3B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NipSnap family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924401.1">
        <property type="nucleotide sequence ID" value="XM_014068926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJQ8"/>
    <dbReference type="GeneID" id="106550939"/>
    <dbReference type="KEGG" id="tsr:106550939"/>
    <dbReference type="OrthoDB" id="10262843at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000017">
        <property type="entry name" value="Protein NipSnap homolog 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF19">
        <property type="entry name" value="PROTEIN NIPSNAP HOMOLOG 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIPSNAP" evidence="2">
        <location>
            <begin position="48"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="NIPSNAP" evidence="2">
        <location>
            <begin position="155"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924401.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="29195" checksum="535DFBBC2AAABF6A" modified="2020-10-07" version="1">MLGLRVGSVLGRLFRRAAAADPFRAQPNVFQACSSFATGPRQEVGTIYELRTYDLKPAKTKEFLELVNKYIKIRMVHSPMIGFWTAEFGAMNKAFHIWKYDNFAHRAAVRKAIINDKEWQEKLAAILPLLDKQHMEIAYMVPWCELGTPPKEGVYELVTYQLKPGGPALWGNSFKAAIDAHVSKGYAKLIGFFHTEYGFLNTVHVLWWFENPDIRAAGRHMAHEDARVVAAVRENIQFLETQRNMLLIPASFSPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKU7</accession>
    <name>A0A6I9YKU7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ZZ-type zinc finger-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">ZZZ3</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Histone H3 reader that is required for the ATAC complex-mediated maintenance of histone acetylation and gene activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. Interacts via (ZZ-type zinc finger) with histone H3 in a methylation-independent manner and acetylation on 'Lys-4' (H3K4ac) moderately enhances the interaction.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924850.1">
        <property type="nucleotide sequence ID" value="XM_014069375.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924857.1">
        <property type="nucleotide sequence ID" value="XM_014069382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKU7"/>
    <dbReference type="GeneID" id="106551293"/>
    <dbReference type="KEGG" id="tsr:106551293"/>
    <dbReference type="CTD" id="26009"/>
    <dbReference type="OrthoDB" id="20473at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02341">
        <property type="entry name" value="ZZ_ZZZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000128">
        <property type="entry name" value="Putative ZZ-type zinc finger-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037830">
        <property type="entry name" value="ZZZ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041981">
        <property type="entry name" value="ZZZ3_ZZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705">
        <property type="entry name" value="ZINC FINGER, ZZ DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705:SF0">
        <property type="entry name" value="ZZ-TYPE ZINC FINGER-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myb-like" evidence="17">
        <location>
            <begin position="644"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="19">
        <location>
            <begin position="652"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="18">
        <location>
            <begin position="815"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="308"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013924850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013924857.1"/>
        </source>
    </evidence>
    <sequence length="900" mass="101496" checksum="6C0E76373663F783" modified="2020-10-07" version="1">MAASRSTRVTRSTVGLNGLDENFCGRTLRNRSIAHPEEVSSLPQVRSRSPKKKSESLQTQKGNSNGKANDVKQQSSKESWVSPRKRGLSTSEKDNVEKQIVENCEKKQVEPLSPVLKRIKRCLRSEAQHSSEEVLPSKTGKDRLEHKHSVSDNVTVNPGPKRACRCLILDDSDKREVKKVTVCAEKLDHSSITEELISCQSINGVNGNGSSASKCGDCQLDGVTAQNSADSCSSEEKSTAKNGNTSSFFHSRNKEDSFIDHSVPCTNSQEQLTCEDHKQIKNCLSEETVNQIQESGTGTGSFCETQSSLLRDSEEEVDVVGYSTASKEQSENTNSNLDSYDNTTASCEPEPPVLDCVSAPISSTSESQEHRYTLRTSPWRAGSTKISPIKQNSPCKENGQLEENNHTVPEKNVESSVTNINGSGANTEIAPSERESYCDSREHPKEGLSKSTSENKLITPSLPSAKESASLHSVDEEEEEPDVYYFESDHVALKHNKDYQRLLQTIAVLEAQRTQAVQDLESLGRHQREALKDPIGFVESLQKKVDVGLPFPQRVVKLPEISWDQYTTSLGNFEREFRNRKRNSRRAKLIFDKGLPTRPKSPLDPRKEGEATSYSVLPFSDCPESSTNNRPQQMIRGRLCDETKPESFNQLWTVEEQKKLEQLLLKYPPEEVESRRWQKIADDLGNRTAKQVASRVQKYFIKLTKAGIPVPGRTPNLYLYSKKSSSRRQHPLNKHLFKPSTFMTSHEPPVFMDEDDDRSCFHSHMANAAEEEPSDEESIPVAYRELPEYKELLKFKKLRKQKVQQMHAESGFVQHIGFKCDNCGTDPIQGVRWHCQDCPPALSLDFCDSCSDCLHETDIHKENHRLEPIYRAEMFLDRDYCMSQGPSYNYLDPNYFPANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLW2</accession>
    <name>A0A6I9YLW2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Glypican-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GPC2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Lipid-anchor</topology>
            <topology evidence="1 12">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925373.1">
        <property type="nucleotide sequence ID" value="XM_014069898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLW2"/>
    <dbReference type="GeneID" id="106551750"/>
    <dbReference type="KEGG" id="tsr:106551750"/>
    <dbReference type="CTD" id="221914"/>
    <dbReference type="OrthoDB" id="10010764at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905475">
        <property type="term" value="P:regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001863">
        <property type="entry name" value="Glypican"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019803">
        <property type="entry name" value="Glypican_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822:SF24">
        <property type="entry name" value="GLYPICAN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01153">
        <property type="entry name" value="Glypican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01207">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 12" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027116950" evidence="14">
        <location>
            <begin position="26"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="337"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925373.1"/>
        </source>
    </evidence>
    <sequence length="530" mass="58614" checksum="DF8F3FF79A8C8DF1" modified="2020-10-07" version="1">MGPPLPLLWALSGLLCAAAAGEARGARSCAESRQALASRGFSLASVPPTLISGEHLRICPQDYTCCASETEERLSEQGQTELRALLGETGAFLLSTLSSRQQRFQDFFQELLAGAERSLQAMFARSYGRLYLQHARLFQGLFVELQHQLKGSRPSLDEALSDFWSQLLERMLPLLNPQYSFPEGYLECVGRQVESLRAFGDSPNQLRVQVSRAFLAARVLVQGLGTGRDVVAQAVKLSPTGECLQAHMRLSFCPLCRGLPALKPCHGFCLNVMKGCLASITEMDAEWGRFLDALTQLAEQLTGPFNFELAADSIAVKISEAIMYLQERNIQTSAKVFQSCGNPRPTPARSRRDPQEGSRHHFHVYPPEEKPTTAGGTNLDRLVLDLKEKLRLMRRFWALLPHTLCSSEKMAADMANEDSCWNGQTRGRYLPDVTADGLVNQINNPEVEVDISQPDLLTRRRLLALRSVASRLLGACHGQDLDFQDTDDDAISGSGAGYHEDRPASPRPYGSSSLILHSTYLSDFPNFKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN20</accession>
    <name>A0A6I9YN20_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106552152</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925828.1">
        <property type="nucleotide sequence ID" value="XM_014070353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN20"/>
    <dbReference type="GeneID" id="106552152"/>
    <dbReference type="KEGG" id="tsr:106552152"/>
    <dbReference type="OrthoDB" id="8190486at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013925828.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="27699" checksum="B1326C4738AA8577" modified="2020-10-07" version="1">MWLNSEERDDSSPELPGNDSEVTQPSTGESGAQGAQPDLNYYMRLLLNHSDFEGSAPQPETCATDSPPRTTNRTQAGNSQSYHERSTALWDPAQPSFSTIRIQAPSSAAVQKTKVSLPKSGPVCLSLEPVSPSRQNVAHEEGILSPNHLAAVSLLRKQYQAKGRPVPSTEELYKYLHRIGQNGPETKSDGNLSARRNLDPKLSESMRKEGPPSRRAGSSGPGREKSHAPGKTGKKLSGSLPSNSRSSSSSRGGSVWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP79</accession>
    <name>A0A6I9YP79_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear transcription factor Y subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CAAT box DNA-binding protein subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nuclear transcription factor Y subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NFYC</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925971.1">
        <property type="nucleotide sequence ID" value="XM_014070496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP79"/>
    <dbReference type="GeneID" id="106552269"/>
    <dbReference type="KEGG" id="tsr:106552269"/>
    <dbReference type="CTD" id="4802"/>
    <dbReference type="OrthoDB" id="1272441at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000006">
        <property type="entry name" value="Nuclear transcription factor Y subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF8">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="29"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925971.1"/>
        </source>
    </evidence>
    <sequence length="334" mass="37035" checksum="C2F55934F189710D" modified="2020-10-07" version="1">MSTDGGFAGGNSDAQQCLQSFWPRVMDEIRNLTVKDFRVQELPLARIKKIMKLDEDVKMISAEAPVLFAKAAQIFITELTLRAWIHTEDNKRRTLQRNDIAMAITKFDQFDFLIDIVPRDELKPPKRQEEVRQAVTPAEPVQYYFTLAQQPAAVQVQGQQTGQQTTTSTTTIQPGQIIIAQPQQGQTTPVTMQVGEGQQVQIVQAQPQGQTQPAQSGTGPTMQVMQQIITNTGEIQQIPVQLNAGQLQYIRLAQPVSGTQVVQGQIQTLAANTQQIAQTDVQQGQQQFSQFTDGQQLYQIQQVTMPAGQDITQPMFIQSTNQTTDGQAPQVTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPQ7</accession>
    <name>A0A6I9YPQ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tektin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TEKT3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms filamentous polymers in the walls of ciliary and flagellar microtubules. Required for normal sperm mobility.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Microtubule inner protein component of sperm flagellar doublet microtubules. Interacts with TEKT1, TEKT2, TEKT4 and TEKT5. Interacts with CCDC38.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
            <location evidence="14">Cytoskeleton</location>
            <location evidence="14">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">Secretory vesicle</location>
            <location evidence="11">Acrosome outer membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925670.1">
        <property type="nucleotide sequence ID" value="XM_014070195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPQ7"/>
    <dbReference type="GeneID" id="106552015"/>
    <dbReference type="KEGG" id="tsr:106552015"/>
    <dbReference type="CTD" id="64518"/>
    <dbReference type="OrthoDB" id="9886517at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002081">
        <property type="term" value="C:outer acrosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF24">
        <property type="entry name" value="TEKTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 14" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 14" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013925670.1"/>
        </source>
    </evidence>
    <sequence length="490" mass="56445" checksum="1CCAF5FBAB310E90" modified="2020-10-07" version="1">MELLGSTLTATYAHPRPTPSNFLPAISTMASSYKNRFPHYSLTHSLSLPWRPSTYYKVAAITPTLAPFSKSSQGVTPSHMLPFVSNRTALFNRYSPDDWYRSNLTNYKDSEASRHNAENMRVDTTRMIQDKEQQTRKTQTETTKNIGERVNDIVFWKTELNHEIDGMIGETNALTEMKKRLERGLVETEGPLQVAQECLLHREKRMGIDLVHDDVEKQLLTEVDVIHSCQERMRRYLDKAMAQLAADRSAQHELEKDLSDKQTAHRIDDKCHHLRNTSDGIGYYRGVERFDATISVPESWAKFTDDNILRSQSERAASSKLRDDIETLLVVTANEMWNQFNRANVAFTNRISETADAKNKIQTHLAKTLQEIFDTEMIIEAIRKAIREKGPSLKVAQTRLDERTRRPNVELCRDYAQLRLVNEVYEIDDTIQGLQQRLRDAEDILQTLLHTKANLEHDLAVKANSLFIDQEKCMGMRKTFPNTLRLVGYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPZ5</accession>
    <name>A0A6I9YPZ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sprouty-related, EVH1 domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SPRED1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926362.1">
        <property type="nucleotide sequence ID" value="XM_014070887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPZ5"/>
    <dbReference type="GeneID" id="106552272"/>
    <dbReference type="KEGG" id="tsr:106552272"/>
    <dbReference type="CTD" id="161742"/>
    <dbReference type="OrthoDB" id="5786858at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10574">
        <property type="entry name" value="EVH1_SPRED-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000052">
        <property type="entry name" value="Sprouty-related, EVH1 domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023337">
        <property type="entry name" value="KBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041937">
        <property type="entry name" value="SPRE_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007875">
        <property type="entry name" value="Sprouty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF18">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05210">
        <property type="entry name" value="Sprouty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51488">
        <property type="entry name" value="KBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51227">
        <property type="entry name" value="SPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="5">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="KBD" evidence="6">
        <location>
            <begin position="200"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013926362.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="47137" checksum="6B26407828B82BE2" modified="2020-10-07" version="1">MNEETVNSNHDNSYARVRAVVMTRDDSSGGWLPLAGGGLSYVTIFKVIHQEESGCADFLIHGERLRDKTVVLECTLKKELIYNKVTPTFHHWKIDEKKFGLTFQSPADARAFDRGIRRAVEDISQGCLTSHDEIEVPADGFPAIPENTSSLLMKDPIFQHDSLASNDPHKTLCIRPSVFEDLNNRRLYFHNQPNQIPQKPVRSVTFQDEEEIVRINPRDILIRRYADYRHPDMWKNDLDRDEIDSSLHCPKADHKKSDYLYPSGDGPKLNSLKDSKSSVVLKTQPSSIKLKKSKKRKEDGERSRCIYCQERFNREENGRGKCQDAPDPIRRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVTLSFIAPCMCCYLPLRACHHCGEICGCCGGKHKAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQI4</accession>
    <name>A0A6I9YQI4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MFSD3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926179.1">
        <property type="nucleotide sequence ID" value="XM_014070704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQI4"/>
    <dbReference type="GeneID" id="106552433"/>
    <dbReference type="KEGG" id="tsr:106552433"/>
    <dbReference type="CTD" id="69572"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17485">
        <property type="entry name" value="MFS_MFSD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000176">
        <property type="entry name" value="Major facilitator superfamily domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF10">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926179.1"/>
        </source>
    </evidence>
    <sequence length="385" mass="42727" checksum="FFCF675305833E5B" modified="2020-10-07" version="1">MARSWLPRMVDQERAMSLKFWFLFLLYFVQGIPYGLQSGLLPIYFRTVGLSFTKISLTKLLYLPWFLKVFSAPFVDWYFSKKAWLIFTMSGLALTCLACSFLTPEVDFLGVAVALLFMNLFASLQDVATDGIAIQMLGREELGFGNSIQVVAYKLGSVLAGGGLLTFLHHLGWGPLFVYLALVYVAASWFTSTFPLRPSPRGSQAEESNLNLLGFFHELLLVPDTLWTAGLVLIYKLGEQGSLSMFPLFLLDHSFSPQKLGFWNGIVATAFSIVGSSLGGQLMSNGRDWDSLQRLLVLRFCNLLFQTWVMVNYTDHAVAFEVAAVLSMCIQHFIGGLITTLVFSKMMLCTQKAPESIQATHYSLLAAIEVLGKVAFSTVSGSLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQV8</accession>
    <name>A0A6I9YQV8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neuritin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NRN1L</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuritin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926030.1">
        <property type="nucleotide sequence ID" value="XM_014070555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQV8"/>
    <dbReference type="GeneID" id="106552311"/>
    <dbReference type="KEGG" id="tsr:106552311"/>
    <dbReference type="CTD" id="123904"/>
    <dbReference type="OrthoDB" id="9929715at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990138">
        <property type="term" value="P:neuron projection extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026144">
        <property type="entry name" value="Neuritin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902:SF2">
        <property type="entry name" value="NEURITIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902">
        <property type="entry name" value="NEURITIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15056">
        <property type="entry name" value="NRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027117256" evidence="9">
        <location>
            <begin position="29"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013926030.1"/>
        </source>
    </evidence>
    <sequence length="169" mass="18520" checksum="BD8A1EBB45D675AE" modified="2020-10-07" version="1">MGCGASLGAWALLLFTLHLVLVGQPVNAAGWKCNTIYKGFAECLVSLGDSMAQNVQKDMEDDTIQDQEELNTICRSWDDFHICATKVLSNCPEEAANIWESLRQESRKIQFQGNLHDLCSSRAQLAGDGKALRVDETNQETLRGAAPLLWPSLMGRLTLVALVALVPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRR8</accession>
    <name>A0A6I9YRR8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Forkhead box protein C2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FOXC2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926831.1">
        <property type="nucleotide sequence ID" value="XM_014071356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRR8"/>
    <dbReference type="GeneID" id="106552947"/>
    <dbReference type="KEGG" id="tsr:106552947"/>
    <dbReference type="CTD" id="2303"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20044">
        <property type="entry name" value="FH_FOXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047391">
        <property type="entry name" value="FOXC1/C2-like_FH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF68">
        <property type="entry name" value="FORKHEAD BOX PROTEIN C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="73"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="73"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="231"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="378"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926831.1"/>
        </source>
    </evidence>
    <sequence length="534" mass="57548" checksum="4048099D35715BAA" modified="2020-10-07" version="1">MQARYSVSDPNALGVVPYLSEQNYYRTAGTYGGMAGSLGVYAGHPEQYAAGLGRSYGPYPGHQPVQAPKDLVKPPYSYIALITMAIQNAPEKKITLNGIYQFIMERFPFYRENKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGSYWTLDPDSYNMFENGSFLRRRRRFKKKDVSKEKEESPREKHHKEPVKAASELAKEPSPPSVSAPVPASSDKKVVIKSEASSPELPVITKVETLSPESGSALQDSPRSVASTPSLSTEGSLPGEPHQAHQPHPHHHPAAGANGLPGFSVENIMTLRTSPGGELSPVGRTPTGLCGSSLPLGYGQAPASSIYSQACSQGGLGEAGGSYQCSMRAMSLYTGERNAHLCGAGSLEEAAASMSEPPPQVANGAPSPLAGLNLAQGGQQQQQETSLPPSNHHHQPAHHHQHHHPLPHHQAPGQAAAAAAAAAAAASWYLNHASEINQPLPSHTFGAQQQSFPNVREMFNTHRLGLETSTALNEQQVSPTTCELSYRAPPSIYRHSAPYSYDCAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSD7</accession>
    <name>A0A6I9YSD7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA replication factor Cdt1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CDT1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927056.1">
        <property type="nucleotide sequence ID" value="XM_014071581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSD7"/>
    <dbReference type="GeneID" id="106553139"/>
    <dbReference type="KEGG" id="tsr:106553139"/>
    <dbReference type="CTD" id="81620"/>
    <dbReference type="OrthoDB" id="341730at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071163">
        <property type="term" value="P:DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045173">
        <property type="entry name" value="Cdt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014939">
        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28637">
        <property type="entry name" value="DNA REPLICATION FACTOR CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28637:SF1">
        <property type="entry name" value="DNA REPLICATION FACTOR CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08839">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDT1 Geminin-binding" evidence="2">
        <location>
            <begin position="156"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927056.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="23271" checksum="9010D47A0627D31B" modified="2020-10-07" version="1">MAEEEEEGAPAGSACPTVSTAALGSPSPSSLDKEAKELVNEMLSPGLKLGGTSVPVASLEEKDAPQKQLLELRGRLGKIHSLVQKIKGGAADLESSGDLKSRLKRARLLESQIRQRKLSPKEERKEPESTAETSEKVPAYQRFHTLAEDVPPSLLLPYKYKVLAEMFRSTDTLVAMMFNRLEAVTFAKVKKGVQEMTRKRFEERHLGQIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSX2</accession>
    <name>A0A6I9YSX2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zonadhesin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106553137</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927054.1">
        <property type="nucleotide sequence ID" value="XM_014071579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSX2"/>
    <dbReference type="GeneID" id="106553137"/>
    <dbReference type="KEGG" id="tsr:106553137"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF374">
        <property type="entry name" value="ZONADHESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="5">
        <location>
            <begin position="465"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="363"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="368"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013927054.1"/>
        </source>
    </evidence>
    <sequence length="700" mass="73144" checksum="4EB61DFE9C289B81" modified="2020-10-07" version="1">MDPFCGWRESMNNTWKRTNQNTPTEDTGPPGDYPHGEGYYIYVEGGQLKPNGSTRLYSSSFCISQEACVEFYYHMHSVVEVETQLQVLLEDPSGSAVLLWNRTGTQSPNWLHGSVTLPYTRAQPSKVVFEVIRGRNAYLDVSLDNILVHKGHCAEHPPTPVPPSTIATAPTTTVGPNITVPTGTATTPIITSTAVGPNITVPIGTAATPITTSTAIQPNITIPTETATTPITTSTAVGPNITVPIGTAATPITTSTAIQPNITVPTGTAATPITNSTAMSPNITVPIGTAATPITTSTAIQPNITVPTGTAATPITNSTAVGPNITVPTGTASTPITTSTAVHPNITVPIGTASTPITTSTAVHPNITVPTGTASTPITTSTAVHPNITIPTGTATTPITTSTAVGPNITIPTGTATTPITTSTAVGPNITIPTRTASTPITTTKPTTQRPGVDQTTPEQPPDSASCIVSGDPHYTTYDGRLFHFMGNCTYLLTATCNATPDQPTFQIQTTNEHRGSNTKVSYLKSVNITVHGTEIVLLKGRRVTVDGKRVTLPVSLAGGRVSVRLSGTFVRVQTDFGLSVRFDGNHHAEVSVPSSYFGKLCGLCGNFNGQAGDDNLMADGTSAGADANQLGKSWQVPNIGDASCTDSKDPEKCHPDIAEEAQGPTSCGILTDPQAFLCVLDLFEIHHFRDVPYLKNICP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT31</accession>
    <name>A0A6I9YT31_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein SSUH2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106553489</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927472.1">
        <property type="nucleotide sequence ID" value="XM_014071997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT31"/>
    <dbReference type="GeneID" id="106553489"/>
    <dbReference type="KEGG" id="tsr:106553489"/>
    <dbReference type="OrthoDB" id="3355217at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052789">
        <property type="entry name" value="SSUH2_homolog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48465">
        <property type="entry name" value="PROTEIN SSUH2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48465:SF1">
        <property type="entry name" value="PROTEIN SSUH2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013927472.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="17167" checksum="38B0022CBEB95359" modified="2020-10-07" version="1">MCNVCSGLGTTACTDCAGTGKLLTYIEMQVEWKNNIFEYVMDERTGFPTELFKGVKGKKLFVDEQYLVHPVVSFPICAINQASRNAIEQHHAQFGSTARILRQRQTIELVFLTKVEYEWHRKSYSYYVYGNEHKVYAEDYPKKCGCTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTT2</accession>
    <name>A0A6I9YTT2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">High mobility group protein 20A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">HMG box-containing protein 20A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HMG20A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013927717.1">
        <property type="nucleotide sequence ID" value="XM_014072242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTT2"/>
    <dbReference type="GeneID" id="106553691"/>
    <dbReference type="KEGG" id="tsr:106553691"/>
    <dbReference type="CTD" id="10363"/>
    <dbReference type="OrthoDB" id="3213154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ChromReg_NeuronalGeneExpr"/>
    </dbReference>
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        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040:SF1">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="11">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="116"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="69"/>
            <end position="101"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039787"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927717.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="27726" checksum="745E31A16F6474CD" modified="2020-10-07" version="1">MNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKRRYLEEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKTHRQEGTRPAPHDHQKEPETKERSVFDIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEKLEVDVIHERSRNTVLQQHLETLRQALASSFAGVPLPGSGEIPTLDTIDSYMNRLHHLILANPQENETLIATVREVVNHLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU63</accession>
    <name>A0A6I9YU63_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">PRKD3</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Activated by DAG and phorbol esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013927180.1">
        <property type="nucleotide sequence ID" value="XM_014071705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU63"/>
    <dbReference type="GeneID" id="106553238"/>
    <dbReference type="KEGG" id="tsr:106553238"/>
    <dbReference type="CTD" id="23683"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20841">
        <property type="entry name" value="C1_PKD3_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14082">
        <property type="entry name" value="STKc_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000151">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000056">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000137">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000019">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015727">
        <property type="entry name" value="Protein_Kinase_C_mu-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000552">
        <property type="entry name" value="PKC_mu_nu_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19 28" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="155"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="269"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="24">
        <location>
            <begin position="415"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="575"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="698"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="581"/>
            <end position="589"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 22">
        <location>
            <position position="604"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013927180.1"/>
        </source>
    </evidence>
    <sequence length="889" mass="100591" checksum="20C4821069B63AF7" modified="2020-10-07" version="1">MSSNESSPPSLQKFSPSVSHAAAPPTPSLSSSPQSGLSGRLSNGSFSTQSLTNSRGSVHGISFLLQIGLTRETVTIEAQDLSLSIVKDLVCSVVYQKFPECGFFGMYDKILLFRHDLNSENILQRITSAEEIREGDLVEVVLSALATVEDFQIRPHMLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGAALSVPRSVQTESTSTEERCHPEPSKRIPSWSGRPIWMEKMVLCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCRFNCHKRCASKVPKDCLGEVVFNGEEPASPGQDTDMPMEVDSSEMNSDVGRGLDYVEEPSPPEDKMFFMDPADLDGDKDEEAVKTISPSTSNNIPLMRVVQSVKHTKRKSSTMVKEGWMVHYTSRDNLRKRHYWRLDSKSLTLFQNESGTKYYKEIPLSEILQVTQPRDFSNMVQGSNPHCFEIITDTMVYFVGENNGSSHHSPVLAATGVGLDVAQSWEKAIRQALMPVTPETSTCITTGQGKDHKELCVSISVSNSQIQENVDISSVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWKEISNEATDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEEHAYEHNLVYPKHFIMAPNPDDMEEDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUI1</accession>
    <name>A0A6I9YUI1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein WWC3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106554155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928238.1">
        <property type="nucleotide sequence ID" value="XM_014072763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUI1"/>
    <dbReference type="GeneID" id="106554155"/>
    <dbReference type="KEGG" id="tsr:106554155"/>
    <dbReference type="OrthoDB" id="2020426at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035330">
        <property type="term" value="P:regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000041">
        <property type="entry name" value="WW and C2 domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051105">
        <property type="entry name" value="WWC/KIBRA_Hippo_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057747">
        <property type="entry name" value="WWC1_hairpin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14791">
        <property type="entry name" value="BOMB/KIRA PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14791:SF25">
        <property type="entry name" value="PROTEIN WWC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25802">
        <property type="entry name" value="WWC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WW" evidence="8">
        <location>
            <begin position="59"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="8">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013928238.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="55595" checksum="99BB601150B304D6" modified="2020-10-07" version="1">MPWLSGGRRRRRQQQQQQQAAAAAPGGSGTGPPPVAPAAAIGGGGGGGSGPRGRESSELPLPAGWEEARDYDGRVFYIDHNTRQTSWIDPRDRITKPLTFADCVGDELPLGWETVYDQQIGVYYMDHINQLTQIEDPREQWRREQERMLKEYLIVAQEALNAKMEIYQIKQQRFELAQEEYQQLNKMCEDDSYSYASTYSGFSTNTKYNPSQIKAEIASRRDRLSRLKRELTQMKQELQYKEKGVETLQEIDRKMSNSHVNYKLDEAQAIMNELRSIRKAICLGERERQDLMQSLAKLTDGFRNSCCITESFHDLQHSSDSFNDANLSQIYCDACSQTDIIGELGLEDTTRHVDKMKLIWQYEETKKRVFRIQQQLALQDQESWPGMAEADIDHLQLIKEKEALLQELQLISQQQRSPEDLKHIEEEKRRLEEDIQQAQATSSHGAAERILSQEKKNCLLMQLEEATRLSSYLCSQLKRLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV21</accession>
    <name>A0A6I9YV21_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18">Zinc finger and BTB domain-containing protein 46 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">ZBTB46</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928453.1">
        <property type="nucleotide sequence ID" value="XM_014072978.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928454.1">
        <property type="nucleotide sequence ID" value="XM_014072979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV21"/>
    <dbReference type="GeneID" id="106554330"/>
    <dbReference type="KEGG" id="tsr:106554330"/>
    <dbReference type="CTD" id="140685"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000045">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF21">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="31"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="562"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="173"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="249"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013928453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013928454.1"/>
        </source>
    </evidence>
    <sequence length="616" mass="68394" checksum="9559B9584C77A201" modified="2020-10-07" version="1">MNHQKEDMEIASHYHHLLQELNEQRQHGILCDVCIIVEGKIFKAHKNILLGSSRYFKTLYCQVQKTSDQATVTHLDIVTAQGFKAIIDFMYSAHLALTSRNVIEVMSAASYLQMTEIVQACHNFIKAALDISIKSDASDDLIDYEFGASSSNSTDALISAVVAGRSISPWLARRTSPANSSGDSAIASCHEGGSTCGKEDQEPKSDSHEDISSQSLWSTDLAYGTLRVKEEQISPAHYGVNELQSAKNSAMQNAFSEQGATDGWQPTRRRKNRKNKDTVRHITQQLGEDSRTTSPVPPFLTAPGWPYSNQEASVDLTGAEPNNSDNRIERVDLYAKVDEALLGGDVSYLSQPLTPEKEDALQALAVANLRAALMSKNSLLSLKADMLGEESSLLFEYLPRGTHSLSWAGTQLNNSRGECNGKMEVPPPQLVSSQLATQISTPIITTSDNVSPETLKRVLSSTQITHDRFLALWRLDGFHTDMNTAQSEDMDLDHEDLFLGFPGPRTLCCTSCRKFFNCLTKLKGHFQEHTFPMIIPLDMTDYPRPNLTGSSEHTSPCHLARFQCSKCPASFTLKSNMDRHEKTTPFNWKKMQCPHGLKLFRDETDLNQHSMSVPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVB5</accession>
    <name>A0A6I9YVB5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">SH3 and PX domain-containing protein 2B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106554418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928548.1">
        <property type="nucleotide sequence ID" value="XM_014073073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVB5"/>
    <dbReference type="GeneID" id="106554418"/>
    <dbReference type="KEGG" id="tsr:106554418"/>
    <dbReference type="OrthoDB" id="9040096at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06888">
        <property type="entry name" value="PX_FISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000005">
        <property type="entry name" value="SH3 and PX domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037961">
        <property type="entry name" value="SH3PXD2_PX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF26">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="9">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013928548.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="21315" checksum="482EB4D3FB23619A" modified="2020-10-07" version="1">MPRKSIVEVKVLDVQKRRIPNKHYVYIIKVTWSNGSTEAIYRRYSKFFDLQMQMLDKFPMEGGQKDPKQRIIPFLPGKILFRRSHIRDVAIKRLIPIDEYCKALIQLPPYISQCDEVLRFFETRPEDLNPPKDEPIGKKRSDFQVQLKFFREKLQLYLLPNSREHSIKEDGNGMFLVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVK8</accession>
    <name>A0A6I9YVK8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Glycerophosphodiester phosphodiesterase domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GDPD1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-O-(1Z-octadecenyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H2O = 1-O-(1Z-octadecenyl)-sn-glycero-3-phosphate + N-hexadecanoylethanolamine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71464"/>
            <dbReference type="ChEBI" id="CHEBI:137009"/>
            <dbReference type="ChEBI" id="CHEBI:137017"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:53185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41143"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:77580"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:41144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N,1-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(9Z-octadecenoyl) ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71466"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85222"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:56461"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45544"/>
            <dbReference type="ChEBI" id="CHEBI:2700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85223"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:45545"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoylethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53168"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71464"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:53169"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928049.1">
        <property type="nucleotide sequence ID" value="XM_014072574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVK8"/>
    <dbReference type="GeneID" id="106553978"/>
    <dbReference type="KEGG" id="tsr:106553978"/>
    <dbReference type="CTD" id="284161"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08612">
        <property type="entry name" value="GDPD_GDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF1">
        <property type="entry name" value="LYSOPHOSPHOLIPASE D GDPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="14">
        <location>
            <begin position="38"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013928049.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="36081" checksum="33B8F2FAD288952D" modified="2020-10-07" version="1">MSTLFYLLSTLAGYLLTSALLLKFPGLLHRRKRHRFRCRHISHRGGAGENLENTMAAFRHAVNVGTDMLELDCHLTKDEQVVVSHDENLKRSTGVDVNISDLKYSELPPYLCKLEVTFQKDCQCEGEDKRIPLLQEVFEAFPHTPVNVDIKVNNDRLIKKVSELVSSYKREHLTVWGSVSHEVVEKCHKENCHIPILFCLRRVLLLLGLFYTGLLPFFPILEDFLEIPMPSIILKLKESEKISKGQRFTIWLIDVLLMRKALFDHLTARGIQVYIWVLNEDQEYKRAFDLGATGVMTDYPTKLKEFLHNYPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWF4</accession>
    <name>A0A6I9YWF4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UPF0669 protein C6orf120 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106554222</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0669 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928321.1">
        <property type="nucleotide sequence ID" value="XM_014072846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWF4"/>
    <dbReference type="GeneID" id="106554222"/>
    <dbReference type="KEGG" id="tsr:106554222"/>
    <dbReference type="OrthoDB" id="10046613at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031420">
        <property type="entry name" value="UPF0669"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31703">
        <property type="entry name" value="UPF0669 PROTEIN C6ORF120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31703:SF2">
        <property type="entry name" value="UPF0669 PROTEIN C6ORF120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17065">
        <property type="entry name" value="UPF0669"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026777256" evidence="6">
        <location>
            <begin position="24"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928321.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="21291" checksum="90E6161F50452CB3" modified="2020-10-07" version="1">MAPYYKRILVILFTFQGLLITSAFNEDGDVPEEWILLHVVQGQIGAGNYSYLRLNHEGKIVLQMQSLKGDADLYVSDVTLHPSFDDYELQSVTCGQDVVYVPAHFRRPVGIGIYGHPSHLESEFEMKVYYDQTVVESPFGGGSYNPEEMKTNQKKFHSTEDEYQDEESVFWTILIGILKLILEILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWX1</accession>
    <name>A0A6I9YWX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thromboxane A2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Prostanoid TP receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106554930</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929153.1">
        <property type="nucleotide sequence ID" value="XM_014073678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWX1"/>
    <dbReference type="GeneID" id="106554930"/>
    <dbReference type="KEGG" id="tsr:106554930"/>
    <dbReference type="OrthoDB" id="5959154at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004957">
        <property type="term" value="F:prostaglandin E receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004960">
        <property type="term" value="F:thromboxane receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15144">
        <property type="entry name" value="7tmA_PGE2_EP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000163">
        <property type="entry name" value="Thromboxane A2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001105">
        <property type="entry name" value="Thbox_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF3">
        <property type="entry name" value="PROSTAGLANDIN E2 RECEPTOR EP1 SUBTYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00429">
        <property type="entry name" value="THROMBOXANER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="206"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="50"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013929153.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="39776" checksum="D79D4CC85A583B94" modified="2020-10-07" version="1">MFAMHPPNASQAPAIHPVNNSSLAHTELVKASSASSPAAPIFSMTLGAISNIVALVILVQSYARFRRRSKATFLLFASSLVITDLAGHVIPGSFVLQLYATRRNWTSMDPSEALCKFFGASMVFFGLCPLFLGFIMAVERCIGITRPLLHAAVVTPGRTKLSLVGLWTIALAIALLPLCSFGKYAVQAQSTWCFIKVQKTEEWTEVAFALLFSLLGLASLVASLICNTFSGLILVRARLRSQRKCGRRRAKAHDIEMVVQLVGIMVVSCICWSPLLVIVILSASNSHKALNYDRLLFLGVRMAAWNQILDPWVYILLRRAVLRKLLALFLRRPDLKTIHFDRWEASSFQSSERSVAAGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWY0</accession>
    <name>A0A6I9YWY0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fibroblast growth factor 11-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106554940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929163.1">
        <property type="nucleotide sequence ID" value="XM_014073688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWY0"/>
    <dbReference type="GeneID" id="106554940"/>
    <dbReference type="KEGG" id="tsr:106554940"/>
    <dbReference type="OrthoDB" id="6158176at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013929163.1"/>
        </source>
    </evidence>
    <sequence length="95" mass="10634" checksum="FED3E41548E3F9A0" modified="2020-10-07" version="1">MAALASSLIRQKREIRDPVASRPVSTQRKVCPRGTKSLCQKQLLILISKVRLCGGRKGRLEKPPDPQLKGIITKLFCRHGFYLQVHPDGSIEGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX57</accession>
    <name>A0A6I9YX57_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Alpha-2-macroglobulin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106554413</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928541.1">
        <property type="nucleotide sequence ID" value="XM_014073066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX57"/>
    <dbReference type="GeneID" id="106554413"/>
    <dbReference type="KEGG" id="tsr:106554413"/>
    <dbReference type="OrthoDB" id="9998011at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF165">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026855933" evidence="6">
        <location>
            <begin position="26"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Macroglobulin" evidence="7">
        <location>
            <begin position="133"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Macroglobulin" evidence="8">
        <location>
            <begin position="227"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013928541.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="43512" checksum="7130A390EA08E2DA" modified="2020-10-07" version="1">MGKSRILAGPCLFLLGLFFLPGGTTTPPPKPQYLVLVPYVVPTETSRKICVQLSHLNESVTLSITLEYGLQNRSLLSEVVSKKDLFQCTDFQLPKWERSFNTHIALFTVEVTGETLRYLDRKRVYIDNQESLIFVQTDKPIYKPGQKVLFRCVSLNKEFIPVNEKVPLIYIEDPKRNRLFQWKDVELKGGLVQLEFPLSSEPALGSYKVVVLKDDERNIHHPFTVDEYVLPKFEVTVKSTPIITILDKEMEVTACGKYTYGKPVPGKVSIRVCRRYSYFRSSCYGEESKAICEEFSGEADVHGCFSHVLDLKIFQLKRNGFQMEIRADGVITEEGTEVKLTGSGTTQITSTISKVVFENVDNYYKPGLPFSGEVRHFIQTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY39</accession>
    <name>A0A6I9YY39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DPH2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929593.1">
        <property type="nucleotide sequence ID" value="XM_014074118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY39"/>
    <dbReference type="GeneID" id="106555297"/>
    <dbReference type="KEGG" id="tsr:106555297"/>
    <dbReference type="CTD" id="1802"/>
    <dbReference type="OrthoDB" id="449241at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010014">
        <property type="entry name" value="DHP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00272">
        <property type="entry name" value="DPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00408">
        <property type="entry name" value="Diphthamide_biosynthesis_famil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929593.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="52837" checksum="1C41DC5641DC5F64" modified="2020-10-07" version="1">MATAFNTDGAEVIQRTLNSTEVGSRPPVLGIEEFYELEKAAAFVSDNGFTKIALQFPDKLLPDSADVAARMEKATGAKIYVLGDTSYGSCCVDEVAAEHVSAEAIVHYGPACLSPCRKLPVLHIFGQEQLDAMRCVEVFQELYPDHQTYVVVLSESAYFHAIDDLASKLQPIYPNVVFAQLDSKKNLDSSQPISGMIQQFGRRFVIDEDHGLENYSMFYIGSEGAELTNFMLSWNQCPFSSFDPRTGQGRCETLDVNRALRRRLYLVERTRDAQVVGIVVGTLGVEDYLSVLEHLRQIIHRAGKRAYTLAVGKPNPAKLANFPEVDIFVLVACPQNSLLNSTDFYRPLITPYELEIACNPARTWTGRYVTDFRHLLPGACDHVVFPEKTNIEPSISLITGQLRSIDPSLIDAEPNSDTTVMCRNHVQTVSQISPAASFLESRSWRGLEQQLGQTEVKKAIVGRRGIAIAYEDEPCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYR9</accession>
    <name>A0A6I9YYR9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ADAMTS-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ADAMTSL2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928720.1">
        <property type="nucleotide sequence ID" value="XM_014073245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYR9"/>
    <dbReference type="GeneID" id="106554545"/>
    <dbReference type="KEGG" id="tsr:106554545"/>
    <dbReference type="CTD" id="9719"/>
    <dbReference type="OrthoDB" id="5781878at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF147">
        <property type="entry name" value="ADAMTS-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PLAC" evidence="7">
        <location>
            <begin position="929"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="72"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="76"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013928720.1"/>
        </source>
    </evidence>
    <sequence length="968" mass="108385" checksum="696E1664D59C55A8" modified="2020-10-07" version="1">MPSPRMRRVKCTWDHRIPHGCSHSKRTFVLALFVVNTALTFSQEKVPASNSLEERDDVTAYWWGEWSKWAACTRTCGGGIRFQERHCLQQRRKLATGFLNKTCNGMPKRYQLCEVQQCPPSGRSFREEQCSSFNSRVYNGHTFHWKPLYPDDYVHISSKPCDLHCTTTDGQRQLMVLARDGTSCKTSSFLGVCVSGKCEPIGCDGVLFSPHALDKCGVCQGNGSSCTHVTGSYRKGNAPLGYSLVTHIPAGATDIQIVERKKSADVLAIADEGGSYFFNGDYRVDIPQNFNIAGTVFKYRRPMDIFETGIEYIVAQGPTNQGLNVMVWNQNGKNPSITYEYILQTRPYLKKLPPLYYGFSESNSKEKTEYEGGFLWGLTQLNASLLATVSQERGHLEKLLFGRQGRGDNLKLSKMQQTNEVYPRIPLEYGPPLPSKPSIESNTTKTNYKVEGNGSKLDSLLPSQEILSENAILHSFGDKRSDSKESIWNKTLNSIFSPVKPINTFRPEIDGLYVDYDDSDELLAYVANGTFMELTHEEASNASSEIQLSNATISSAMGNRTQKTRNQLKLFRKGQNMSPADMYRWKLSSHEPCSSTCTTGVMSTYAMCIRYDGTEVEDPFCDAATRPEPIHEFCAGRECQPRWETSSWSECSRTCGEGHQFRIVRCWKMLSLGLDSSVYSNLCESADIARPEERKVCKNPACGPQWEMSEWSECTARCGERSMVSRDIRCSEEERLCDANTKPAADKNCTGPPCDRQWTVSDWGPCSGGCGPGRMIRQVYCKSSDGRVVPETQCNPEAKPLAIHPCGEKNCPAGWLAQDWERCNATCGRGLKKRTVLCLEIVSGKIKTRPPATCDATKKEAEEATCFERPCFKWYTTPWSECTKTCGIGVRMRDVKCYQGKDLVRGCDPLLKPVAKQTCDLHPCPTEPPDDSCQDQAGTNCALAIKVNLCGHWYYSKACCRSCRVPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZB7</accession>
    <name>A0A6I9YZB7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Collagen alpha-6(VI) chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106555138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929389.1">
        <property type="nucleotide sequence ID" value="XM_014073914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZB7"/>
    <dbReference type="GeneID" id="106555138"/>
    <dbReference type="KEGG" id="tsr:106555138"/>
    <dbReference type="OrthoDB" id="6380629at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF3">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXV) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013929389.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="26076" checksum="1C56A1E76011A668" modified="2020-10-07" version="1">MGHPGYPGNDGDPGPRGILGIKGKQGDIGECGTRGQKGQRGLHGKRGEDGVDGFDGIHGDEGSLGFPGLKGKKGDPGEMGNTGLKGPPGDQGPKGFQGDHGEPGQDNNIRGAKGFKGLSGRMGERGLKGSDGSGGSSGNKGAEGNQGPLGPKGEEGNPGSDGLQGDQGVQGFLGTTGILGVKGEKGQPGNLGRLGGIGDEGPKGNQGKLGSRGNKGESGEPGEKGEKGSSGQRGMKGEEGTVGFGNIGRKGKKGIEGFPGDCGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0K9</accession>
    <name>A0A6I9Z0K9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SURP and G-patch domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106555317</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929616.1">
        <property type="nucleotide sequence ID" value="XM_014074141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0K9"/>
    <dbReference type="GeneID" id="106555317"/>
    <dbReference type="KEGG" id="tsr:106555317"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040169">
        <property type="entry name" value="SUGP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23340">
        <property type="entry name" value="ARGININE/SERINE RICH SPLICING FACTOR SF4/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23340:SF3">
        <property type="entry name" value="SURP AND G-PATCH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SURP motif" evidence="6">
        <location>
            <begin position="64"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="6">
        <location>
            <begin position="142"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013929616.1"/>
        </source>
    </evidence>
    <sequence length="293" mass="32778" checksum="02C5966D4C67C5EB" modified="2020-10-07" version="1">MGKRPNLSVSSMLNQVKNYTHSKQTPVTNRPSVFQSPDDEEEQDYEQWLEIKICPPEDIRTRRIVEKLARVVAEGGPEMEKVALEDCKDNPVFSFLHDKNSPEFLYYRKKLAEMWKKGQDVEDTTPPQKKVSPPEDEETKDCAEKLARFVADGGPVVEMVALKNHRENQSFRFLYEPNSSGYKYYQQKLEEFRKANSDIEGMPPISEANLKRKASSEGGPSSSISSSSSFSSSSSSSSSTTAVPTVSLPKQAAASSAKKKRKSRWGPEEEKVDLPPPELAQQTQEPSPTPLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0M8</accession>
    <name>A0A6I9Z0M8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ran-binding protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RANBP9</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930548.1">
        <property type="nucleotide sequence ID" value="XM_014075073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0M8"/>
    <dbReference type="GeneID" id="106556104"/>
    <dbReference type="KEGG" id="tsr:106556104"/>
    <dbReference type="CTD" id="10048"/>
    <dbReference type="OrthoDB" id="25503at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="174"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930548.1"/>
        </source>
    </evidence>
    <sequence length="428" mass="47446" checksum="001FA8E07048ECC6" modified="2020-10-07" version="1">MGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDVIGCCVNLINNTCFYTKNGHSLGIAFTDLPPNLYPTVGLQTPGEVVDANFGQHPFVFDIEDYMREWRTKTQAQIDRFPIGDREGEWQTMIQKMVSSYLVHHGYCATAEAFARSTDQTVLEELASIKNRQRIQKLVLAGRMGEAIETTQQLYPSLLERNPNLLFALKVRQFIEMVNGTDSEVRCLGGHSPKSQDSYPVSPRSFSSPSMSPSHGMNIHSLSTTGKGSSTHCSGFENSCSNGVISNKVHQSYCHSKHQSTSLSVPELNNINVTRSQQINNYSSNDVDMETDHYSNGVAETSSNGFLNGSSKHDHEMEECDTEMEVDSSQLRRQLCGGSQAAIERMIHFGRELQAMSEQLRRECGKNSQNKKMLKVSHVTFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0Q1</accession>
    <name>A0A6I9Z0Q1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">SLC6A15</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="32">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-alanine(in) + Na(+)(in) = L-alanine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29283"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-asparagine(in) + Na(+)(in) = L-asparagine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:71383"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-cysteine(in) + Na(+)(in) = L-cysteine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68232"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-glutamine(in) + Na(+)(in) = L-glutamine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68236"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-isoleucine(in) + Na(+)(in) = L-isoleucine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29275"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-leucine(in) + Na(+)(in) = L-leucine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29263"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-methionine(in) + Na(+)(in) = L-methionine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68240"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-phenylalanine(in) + Na(+)(in) = L-phenylalanine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68244"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-pipecolate(in) + Na(+)(in) = L-pipecolate(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:71387"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:61185"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-serine(in) + Na(+)(in) = L-serine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29575"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonine(in) + Na(+)(in) = L-threonine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69999"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-valine(in) + Na(+)(in) = L-valine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29267"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A15 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929874.1">
        <property type="nucleotide sequence ID" value="XM_014074399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0Q1"/>
    <dbReference type="GeneID" id="106555529"/>
    <dbReference type="KEGG" id="tsr:106555529"/>
    <dbReference type="CTD" id="55117"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005298">
        <property type="term" value="F:proline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015820">
        <property type="term" value="P:L-leucine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11522">
        <property type="entry name" value="SLC6sbd_SBAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042934">
        <property type="entry name" value="B(0)AT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002438">
        <property type="entry name" value="Neutral_aa_SLC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF101">
        <property type="entry name" value="SODIUM-DEPENDENT NEUTRAL AMINO ACID TRANSPORTER B(0)AT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01206">
        <property type="entry name" value="ORPHTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="27" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 27" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 28" id="KW-0769">Symport</keyword>
    <keyword evidence="5 28 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 28" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="567"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="613"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="29">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00034276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00034282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00034289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_013929874.1"/>
        </source>
    </evidence>
    <sequence length="728" mass="81330" checksum="1811219FC744B6E5" modified="2020-10-07" version="1">MPKNSKVVKREIVDDVIESVKDLLSNEDSVEDAFKKSELSVNDPEDKDVDVEEGSDVEDERPAWNSKLQYILAQVGFSVGLGNVWRFPYLCQKNGGGAYLVPYLILLLIIGIPLFFLELSVGQRIRRGSIGVWNYISPKLGGIGFASCIVCFFVALYYNVIIGWSLFYFSQSFQHPLPWDQCPLVKNSSHTFVEPECEKSSATTYYWYREALNISNSISESGGLNWKMTICLLAAWVMVCLAMIKGIQSSGKVMYFSSLFPYVVLLCFLIRGLLLNGSLDGIRHMFTPKLEIMLEPKVWREAATQVFFALGLGFGGVIAFSSYNKRDNNCHFDAVLVSFINFFTSVLATLVVFAVLGFKANIINEKCIVQNSQQIISLLNSGNLSKAIIPHHINLSSITAEDYNLVYDIIQKVKEEEFPLGLKPCQIEDELNKAVQGTGLAFIAFTEAMTHFPASPFWSVMFFLMLVNLGLGSMFGTIEGITTPIVDTFKVRKEILTVTCCLLAFCIGLIFVQRSGNYFVTMFDDYSATLPLLIVVTLENIAMSYVYGIDKFMEDLKDMLGFSPNQFYYYTWKYISPIVLLSLLVASIIQMVLSPPSYSAWIEDKASKELQNYPTWGLIVCFSLIALAMLPVPVVYLIRRCNLIDDTSGSLAAVSYKRGHIIKEPVNLEGDDTSLIHGKSPSEGPFPTFGKNIYRKQSGSPAVDTAPNGRYGIGYIMANMSDMPESDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z128</accession>
    <name>A0A6I9Z128_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dynein heavy chain 5, axonemal-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106555268</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929545.1">
        <property type="nucleotide sequence ID" value="XM_014074070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z128"/>
    <dbReference type="GeneID" id="106555268"/>
    <dbReference type="KEGG" id="tsr:106555268"/>
    <dbReference type="OrthoDB" id="424310at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF13">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="3">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="63"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929545.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="14912" checksum="EF11B6BE7314AF64" modified="2020-10-07" version="1">MVQCMGSFQDGVAEKCVEYFQRYRRSTHVTPKSYLSFIQGYKAIYKEKHAEVQTLAHRMNTGLEKLKEASESVAALSKELEAKEKELKIANEKADMVKSSVFISTLLLMGKTGRKLVSRLLLIGALLHMLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z161</accession>
    <name>A0A6I9Z161_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Voltage-dependent L-type calcium channel subunit alpha-1C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Voltage-gated calcium channel subunit alpha Cav1.2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">LOC106556276</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm. Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm. Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Cell membrane</location>
            <location evidence="23">Sarcolemma</location>
            <location evidence="23">T-tubule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Membrane</location>
            <topology evidence="32">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Postsynaptic density membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930748.1">
        <property type="nucleotide sequence ID" value="XM_014075273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z161"/>
    <dbReference type="GeneID" id="106556276"/>
    <dbReference type="KEGG" id="tsr:106556276"/>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000007">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000062">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF10">
        <property type="entry name" value="VOLTAGE-DEPENDENT L-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 30 32" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 32" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="7 32" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19 31" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="30" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 33" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="13 32" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="34">
        <location>
            <begin position="13"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="31">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="36">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="36">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00024012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00024028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00024095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00030570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00045450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="RefSeq" id="XP_013930748.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="27644" checksum="C69284E18F7BF9ED" modified="2020-10-07" version="1">RSPYAITFKNASTVKIIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGVNSLGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYLTGIATDTPAEEEPAPCAPVSQHGRQCQNGTDCRAIWEGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFLVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1Z1</accession>
    <name>A0A6I9Z1Z1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Ribonuclease H1</fullName>
            <shortName evidence="15">RNase H1</shortName>
            <ecNumber evidence="6 15">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">RNASEH1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="6 15"/>
    <dbReference type="RefSeq" id="XP_013931068.1">
        <property type="nucleotide sequence ID" value="XM_014075593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1Z1"/>
    <dbReference type="GeneID" id="106556606"/>
    <dbReference type="KEGG" id="tsr:106556606"/>
    <dbReference type="CTD" id="246243"/>
    <dbReference type="OrthoDB" id="407198at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09280">
        <property type="entry name" value="RNase_HI_eukaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000049">
        <property type="entry name" value="Ribonuclease H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.970.10:FF:000001">
        <property type="entry name" value="Ribonuclease H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050092">
        <property type="entry name" value="RNase_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017067">
        <property type="entry name" value="RNase_H1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642:SF26">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036852">
        <property type="entry name" value="Ribonuclease_H1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="17">
        <location>
            <begin position="143"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR036852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013931068.1"/>
        </source>
    </evidence>
    <sequence length="293" mass="33029" checksum="D299C0FFD6332A70" modified="2020-10-07" version="1">MWRRIIALIVHPFSPSSSGALGLNGGMFYAVRRGRRVGVYGTWDECNEQVHKYQCASFKKFATKKEAWAFINEEATGSSTCSRDFGEKDNNYTHNKDTSESSDTRSYKRSYEEPSEKKYTYKRVKYTDVPYSPPADKNEFKYLGDYTVVYTDGCCSSNGRQEARAGTGVYWGPGHPLNNSERLQGRQTNQRAEINAACKAIEQAKSQNIKKLAIYTDSKFTINGVTNWIPNWKTNGWKTSAGKDVTNREDFERLAKLSEDMDIQWMHVPGHAGFPGNEAADRLAKEGAGKSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2Z7</accession>
    <name>A0A6I9Z2Z7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GTPase-activating protein and VPS9 domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931017.1">
        <property type="nucleotide sequence ID" value="XM_014075542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2Z7"/>
    <dbReference type="GeneID" id="106556559"/>
    <dbReference type="KEGG" id="tsr:106556559"/>
    <dbReference type="OrthoDB" id="9040486at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903479">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05129">
        <property type="entry name" value="RasGAP_RAP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000009">
        <property type="entry name" value="GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF14">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931017.1"/>
        </source>
    </evidence>
    <sequence length="686" mass="75150" checksum="889624B689DF6014" modified="2020-10-07" version="1">MAEDESLLLQVLRYLIEFELKESENPRRLLRKGTCAFSIFFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFTPAQQEKLFGEKGSERFRQKVQEMVESNEAKLVALVNTFIGYLKQNTYCFPHSLRWIMSQMYKTLSCVERLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMIGSEDGDPRLPKANLGKFDKGCVASFLDVVIGGRAVETPPMSSINLLEGLSRTMVYMTYSQLTTLVNFMRNVMSGDQLKEDRVALENLLLNLPQNKPGKSGSQELTPYNTPQLSPATTPASKKNRLALATRSRSRSHIQMDQHPDYDGFSQETIQEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGSQGDVPVDDSKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSESGAGPSGSNGVEALQLLEREQATTQDNLDDKLRKFEIRDMMGLTEDRDISETVSETWSTDVLGSDFDPNIDEDRLQEITGAAAENVLGSLLCLPGSGSVLFDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGVGSASDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDEAVHGASHLTSPPCPADSLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z325</accession>
    <name>A0A6I9Z325_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Low-density lipoprotein receptor class A domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LDLRAD3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931488.1">
        <property type="nucleotide sequence ID" value="XM_014076013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z325"/>
    <dbReference type="GeneID" id="106556959"/>
    <dbReference type="KEGG" id="tsr:106556959"/>
    <dbReference type="CTD" id="143458"/>
    <dbReference type="OrthoDB" id="9988974at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000104">
        <property type="entry name" value="Low-density lipoprotein receptor class A domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF24">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR CLASS A DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="271"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013931488.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="38637" checksum="4C474695036E3814" modified="2020-10-07" version="1">MWLLWLFLGSAESQLLPWNNFTNECNIPGNFMCSNGRCIPGAWQCDGLPDCFDESDEKECPKARSKCGSTFFPCSSGIHCIIGRFRCNGFEDCPDGSDEENCTANPLLCSTDRFHCRNGLCIDKSFMCDNQNNCQDNSDEESCGNSHEADSNQNYVTTEPQLIFYPSVTYAIIGSSAIFVLVVALLALILHHQRKRNNLMTLPVHRLQHPVLLSRLVVLDHPHQCNVTYNVNNGVQYMSTQGYHRPPDLDSPPSYSEAVLDQSRPPWFDLPPPPYSSEIGSRNHLDLPPYRSRTGSVVSTDASETRGPETLDSSPSRNLFTGMEHHSTLREITSEPSDSLINSVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z356</accession>
    <name>A0A6I9Z356_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Zinc finger protein 708-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC106555978</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930405.1">
        <property type="nucleotide sequence ID" value="XM_014074930.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013930406.1">
        <property type="nucleotide sequence ID" value="XM_014074931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z356"/>
    <dbReference type="GeneID" id="106555978"/>
    <dbReference type="KEGG" id="tsr:106555978"/>
    <dbReference type="OrthoDB" id="9041718at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001747">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000136">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000258">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000389">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001868">
        <property type="entry name" value="Zinc finger protein 264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001343">
        <property type="entry name" value="Zinc finger protein 568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001730">
        <property type="entry name" value="zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF100">
        <property type="entry name" value="ZINC FINGER PROTEIN 501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013930405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013930406.1"/>
        </source>
    </evidence>
    <sequence length="609" mass="69356" checksum="F0E62A582B7A046F" modified="2020-10-07" version="1">MNTSGDRTEKPAVQMKLESYERSHSNDWKEESSSSIDAPMQDFLAECGKIKNKDFGESTRPFSGKLDVNEHYPTKTKGNDYICTDNGKNYNCTFIPSHGTGSLTSPKMIHTAKKPYKCMECGNSFSRRSYFTSHKRIHTGEKPYKCVECGKTFIQSSHLTSHKRIHTGEKPYKCMECGKCFTNSSHLTSHKKIHLGEKPYKCSECGKAFCENSSLMRHKRIHTGEKPYKCVECGKVFSQNSSFTFHKMIHAGKRPYKCMECGKTFIQSSNLTCHKRIHTGEKPYKCRVCGRSFTNSSDLISHNWIHSEDKPYKCMECGKTFIQSSHFNSHKRIHAGEKPYKCMECGKGFSYHSTLTSHKRTHTGEKPYKCLECGKSFCESGSLTVHKRIHSGEKPYTCMECGKSFCESGSLTVHKRIHTGEKPYECVDCGKGFCQKASLMRHTRIHTGERPYICTECGKGFTNSSHLNSHKRTHTGEKPYKCPECGKGFTNSSHLNSHKRTHTGEKPYKCLECGKGFTQRGNLNSHKRIHTGEKPYEYAEHGKSFRMTNDLISHTQIHLVRDTQWNENESDPSVLTLQMIKQGAPLEITEDQKNMKSRNIITKSCFSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3I8</accession>
    <name>A0A6I9Z3I8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">A disintegrin and metalloproteinase with thrombospondin motifs 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106556092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930536.1">
        <property type="nucleotide sequence ID" value="XM_014075061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3I8"/>
    <dbReference type="GeneID" id="106556092"/>
    <dbReference type="KEGG" id="tsr:106556092"/>
    <dbReference type="OrthoDB" id="5855429at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000011">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF158">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PLAC" evidence="9">
        <location>
            <begin position="132"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013930536.1"/>
        </source>
    </evidence>
    <sequence length="326" mass="36047" checksum="A93949A6A2176AE3" modified="2020-10-07" version="1">MVQRGFCDASKKPKPIRRMCNLQECMQPFWVAEEWEHCTKTCGTSGYQIRSVRCVQPLHDGANRSVHFKYCSGERPESRRPCNRAPCPAQWKTGPWNPCSVTCGEGTESRQVSCRTGDQCDGEKPESMRVCKLAPCNDEACQGDKSIFCQMEVLARYCSIPGYKKLCCESCGKRSGNLPLAFYSEAAETEEGFITSPAVLPKSSAATTSLAPPRPERQSAPGRMSLAEDHTQVLLPPIYRKAQAAHNPSHRKFRNQASNKAPGPPALHQNLSTNNGLHRSSPLFDTGPVAAHRPGSVDIHSSSRTPRRNEKQAERQPPLRSPTIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3I9</accession>
    <name>A0A6I9Z3I9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106556321</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930794.1">
        <property type="nucleotide sequence ID" value="XM_014075319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3I9"/>
    <dbReference type="GeneID" id="106556321"/>
    <dbReference type="KEGG" id="tsr:106556321"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930794.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="23309" checksum="76B8727526434019" modified="2020-10-07" version="1">MATDLEDLGGDGWKNPGEQVPSRRLAPRATSAGNSRSRLTAQPLLRLQGSGGPLLPGSHGHAGGGLPRGHHGYRHRHDHQMALHCGMPGPGALLPQEFLHRPLLRHPAWPGSVRHQTAVGAHLTEAPLPFQALWRPGTPSPNTASSLPTRLCMTVVSGDGQDLGLQTAPAIAMPPPSMLDWGPGRGCLCTSLPAEGALGHLLKEGQGADRHQLGFYWFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4I4</accession>
    <name>A0A6I9Z4I4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat domain-containing protein 45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ANKRD45</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930765.1">
        <property type="nucleotide sequence ID" value="XM_014075290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4I4"/>
    <dbReference type="GeneID" id="106556295"/>
    <dbReference type="KEGG" id="tsr:106556295"/>
    <dbReference type="CTD" id="339416"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039323">
        <property type="entry name" value="ANKRD_45/46/60"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22677">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22677:SF4">
        <property type="entry name" value="USHER SYNDROME TYPE-1G PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930765.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="27622" checksum="F1A83371A4794666" modified="2020-10-07" version="1">MEPLEETASSNEHEDLGPQEEPAQFNPLLMAALRGDMEDLQQIFEDSEDPDHEKVNKFLMEKDILGRNLLFTTCMVGQNDVIQSLAKYGVDLKDKTVRGYTLLHCAAAWGQLNTLKTLIELEADIYATTFRGENAREIANRYKQAECAEFLDWAEAKQNLRNFISQIQATITDPEKVQGKLNKEDKTTSLKACQAKLDWLENTKEPNTQDFIDQKQQLEDIMLPIFTKLAAPRGQDPVVSKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z606</accession>
    <name>A0A6I9Z606_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 22-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106557398</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932107.1">
        <property type="nucleotide sequence ID" value="XM_014076632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z606"/>
    <dbReference type="GeneID" id="106557398"/>
    <dbReference type="KEGG" id="tsr:106557398"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000111">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000367">
        <property type="entry name" value="Zinc finger protein 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000495">
        <property type="entry name" value="zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="12"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="290"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013932107.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="38261" checksum="3BFD42E61896CBD8" modified="2020-10-07" version="1">MISLLEQREELQAVNENLGQEPEEKEIRQDAHTEDGMTMENDDWEEEKEEEEKEEGSPVVHEGQDLKVPPQVLAWEEACEALQSRSIQDGDRKARSKREKTSRGRCSWGNQHCRPGYRCAGTASPRFFDCPDCGKRFTLSSHLIRHQRVHTGERPFGCQSCTKSFSQRSDLVRHERTHAGIKLYRCSQCDKSFSESSHLIRHQMIHSGEKPFKCNVCGKRYGDSSYLTVHQRAHTGARPYRCVRCGKSFGRSSTLIRHQQVHGDPAPFPGDKPRPRGIWTPFACLPGDEDTEPLLSPQRRPTSPTPSLASSVLSLAPPPQMDPRSMRIMGWQWGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7K5</accession>
    <name>A0A6I9Z7K5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sperm flagellar protein 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106557162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931795.1">
        <property type="nucleotide sequence ID" value="XM_014076320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7K5"/>
    <dbReference type="GeneID" id="106557162"/>
    <dbReference type="KEGG" id="tsr:106557162"/>
    <dbReference type="OrthoDB" id="62528at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002177">
        <property type="term" value="C:manchette"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054517">
        <property type="entry name" value="SPEF2_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052634">
        <property type="entry name" value="Sperm_flagellar-bone_growth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14919">
        <property type="entry name" value="KPL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14919:SF0">
        <property type="entry name" value="SPERM FLAGELLAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22946">
        <property type="entry name" value="SPEF2_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="314"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="380"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931795.1"/>
        </source>
    </evidence>
    <sequence length="686" mass="80174" checksum="B9D16CA5CA0F09E3" modified="2020-10-07" version="1">MTGILCDWLNREVKLSRTVDTESFAREFSSGYLIGELLNKYELQEDFDQFSQSRLANAKLNNFSRMEPTLQLLGVQFDQNVARNIMTEQHGAAIKLVYQLYVVLEKKKKAGLTGVAMDAMRPSANAKLKSVGTEIYRERLKTLVPRQADLSLQQISDSFQSKAKLLESKIVEMQLVKQQQAKQIQEGKKVEELEKKIAEKRRQNEIMAKIQAAVIQIPKPLPQQTDKVIEDQKLQKKKKEAEITYTEIRNFEKQMKKEAVVPAKLTESMLNALKQAQELATAKPVITDLLNTYSDDEYIRKIQKRLEEDTFAREQREKRRRKMLREQLIAHEAQEEAYREEQLVNRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIHREKQFEERRLKEFQEALDREEALAKQEKIELAEQALREKQLHTKLAAERAEARYKKHYDICWEVVEQIIDLATKIGEYRTLTNNLIPAKIMRDWKELFLKGKPIYEQADVSDLSDEPTPEQLVELDKINLLDEKDYDEYKAMVEEWYPPEEIRINKPPPDNVILGHVIHRLMGLVYPPEPEPPPSIFSPFPLKGCILGKLYSGKTTCLNFLEKVFNIQVLSLDALISEAIKSFHEREMSIENLLPIRMKDSSLNQMHVLKEASKASLIVAKTYESAEQNIEIVEPKAFYSCSAWCRSRDMVKERKKHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZ78</accession>
    <name>A0A6I9WZ78_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">STK4</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013908264.1">
        <property type="nucleotide sequence ID" value="XM_014052789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZ78"/>
    <dbReference type="GeneID" id="106538332"/>
    <dbReference type="KEGG" id="tsr:106538332"/>
    <dbReference type="CTD" id="6789"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21887">
        <property type="entry name" value="SARAH_MST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06612">
        <property type="entry name" value="STKc_MST1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000075">
        <property type="entry name" value="Serine/threonine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000410">
        <property type="entry name" value="Serine/threonine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.170.10:FF:000002">
        <property type="entry name" value="serine/threonine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4270:FF:000004">
        <property type="entry name" value="Serine/threonine-protein kinase 3/4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.170.10">
        <property type="entry name" value="p53-like tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024205">
        <property type="entry name" value="Mst1_2_SARAH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036674">
        <property type="entry name" value="p53_tetramer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011524">
        <property type="entry name" value="SARAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF2">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11629">
        <property type="entry name" value="Mst1_SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50951">
        <property type="entry name" value="SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="14 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="190"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="SARAH" evidence="22">
        <location>
            <begin position="591"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013908264.1"/>
        </source>
    </evidence>
    <sequence length="645" mass="73823" checksum="4E033CD55EB3E4C2" modified="2020-10-07" version="1">MFLGRIPPVLDTFCLTYFTGGWVRRIYNLQVIKSQLEDDAFYECQVGPTEKSTGIISHRVQLTVLGKYSWSHAALGRSLELNFDYSSAAKLDFFESRISIVVLLCGPVEAVSIYFKDLMHDFHVGYSDTAWISSIMLAMLYGTGVGMDQTQEKLLVPLLLQSGKIGEKGIREQLRKLDEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKETGQVVAIKQVPVESDLQEIIKEISIMQQCDSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLMEDEISTIIQSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCLADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDEFTDFVKQCLVKCPEQRATATQLLQHPFVKNAKGVFILRDMINEAMDIKLKMQQAQQRDMFHDDEENSEEDEIDSRTMVRARGQEMGTIRAACGMSEGANTMIEYNDTLASQLGTMVINAEDEEDEGTMKRRDETMQAVRPSFLDYFEQKEKENWQNNYGRQAPERCSSDWRVPLDGNYELLRNWTVDELQRRLGTLDPMMEQEIEEVRQRYQSKRQPILDAIEAKKRRQQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZZ4</accession>
    <name>A0A6I9WZZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Small integral membrane protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SMIM5</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907072.1">
        <property type="nucleotide sequence ID" value="XM_014051597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZZ4"/>
    <dbReference type="GeneID" id="106537459"/>
    <dbReference type="KEGG" id="tsr:106537459"/>
    <dbReference type="CTD" id="643008"/>
    <dbReference type="OrthoDB" id="8789646at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd20254">
        <property type="entry name" value="CASIMO1_SMIM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047133">
        <property type="entry name" value="SMIM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031671">
        <property type="entry name" value="SMIM5/18/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37344">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37344:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15831">
        <property type="entry name" value="SMIM5_18_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013907072.1"/>
        </source>
    </evidence>
    <sequence length="80" mass="9242" checksum="1890DC0090E4DE7F" modified="2020-10-07" version="1">MSFKEFQNELIAIGYKLWAKLQRLPKADPLEIVCFFIIILFIVTMLSMMIVACSFCCSCFYDGKPKEKAKRIEVQPADDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X070</accession>
    <name>A0A6I9X070_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pro-adrenomedullin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ADM</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ADM function is mediated by the CALCRL-RAMP2 and CALCRL-RAMP3 receptor complexes with ADM showing the highest potency for the CALCRL-RAMP2 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenomedullin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907157.1">
        <property type="nucleotide sequence ID" value="XM_014051682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X070"/>
    <dbReference type="GeneID" id="106537524"/>
    <dbReference type="KEGG" id="tsr:106537524"/>
    <dbReference type="CTD" id="133"/>
    <dbReference type="OrthoDB" id="8771893at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031700">
        <property type="term" value="F:adrenomedullin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990410">
        <property type="term" value="P:adrenomedullin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010460">
        <property type="term" value="P:positive regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003073">
        <property type="term" value="P:regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051665">
        <property type="entry name" value="Adrenomedullin-reg_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001710">
        <property type="entry name" value="Pro-ADM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414">
        <property type="entry name" value="ADRENOMEDULLIN, ADM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414:SF3">
        <property type="entry name" value="PRO-ADRENOMEDULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00801">
        <property type="entry name" value="ADRENOMEDULN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027094691" description="Pro-adrenomedullin" evidence="12">
        <location>
            <begin position="22"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="99"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR621116-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013907157.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="16932" checksum="111077148F845A7A" modified="2020-10-07" version="1">MKALSLTLLYLGSVLFFGVDATKLDIASNFKRKWITKLMSRARRDLPAQVAAESLHWNLPKQVADPRVPSFIRTQDVKDILQQPSLESRRIRAKREPACKLSTCVTQDLVKRLNQWTDSSKDLSAPPRKLTISGYGRRRRSFAFFLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1J6</accession>
    <name>A0A6I9X1J6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">GPI ethanolamine phosphate transferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PIGO</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909124.1">
        <property type="nucleotide sequence ID" value="XM_014053649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1J6"/>
    <dbReference type="GeneID" id="106538985"/>
    <dbReference type="KEGG" id="tsr:106538985"/>
    <dbReference type="CTD" id="84720"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16023">
        <property type="entry name" value="GPI_EPT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000025">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037675">
        <property type="entry name" value="PIG-O_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="455"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="564"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="674"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="706"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="799"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="862"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="901"/>
            <end position="920"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013909124.1"/>
        </source>
    </evidence>
    <sequence length="937" mass="103152" checksum="0E948CA1A4552FC8" modified="2020-10-07" version="1">MQRLKALTTGSLPTFVDAGSNFASYAVREDNLLWQLASNGKKVVFMGDDTWEGLFPQAFHRAHFFPSFNVKDLHTVDDGILQHLYQTVDSGEWDLLIAHFLGVDHCGHKHGPDHPEMAKKLSQMDEMLSSLVDHLGNDTLLLVAGDHGMTATGDHGGDSDEELDAALFVYSKSPLFQDPPPEEPQTVPQVNLVPTLALLLGVPVPYSNIGEVMADLFATEGDAASSLRAQLAAYDINARQVDRFLNSYAEAAQDLPVEKLRHLQRLFRSAVEEHERLSAQEASLALLPQMEGLRARFQQYLREARAVCAESWARFHPGFMVAGCALLTSSCLLCYVASRLATRLDFSYRHFFLYPSLWGMAGVALLGLAHLLRVARADLLLLSAWGAAASQLGFFWHCWTRRPPAPKVAGLCPPFEGFSLKPRLWAGPGLSLGILFLRCAAMLSDSFVVAEGQVVPFLLTSLVLLQALKLQWKGRLDGSLHCWQLLATLGVVVVCARLSGLFRKCREETPACQTASILGALSSMQEPRAKNLYYGVCLGVLASLVWGTRCWLRHYGNLNSPCALVLFVRWGFPLLALALAGYWAITSGAEETLVKRHAWAQLALEAFPRGIYFLGVAGLLLVLWNPVTVFLEGGQEPPLVSSSSEPLIPQLYRRMQESLKSRLEESPGGCKATVAAYGLGSVYSAALLISLALLAFLLMTLHTERLSLAFLLLFVEAFALLWMNACARALSSSSSTHSDPFEVPWDALVAWALAAAQFFYATGHQPVFPAIHWNAAFVGFPQGHSTNLLPALLVGANTFASHILLAAGCPLLLLWPFVCEAGTSVARRPKTRLQERPEEQMMEMRLREAPERFSAALLQLGLRYLLILGLQLLASVLAAMILRRHLMVWKVFAPKFLFEAVGFVVSCVFLLLGLGLVMRVDCAVSKWFKQVILAHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3L7</accession>
    <name>A0A6I9X3L7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">sphingosine kinase</fullName>
            <ecNumber evidence="7">2.7.1.91</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SPHK2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.91" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013908437.1">
        <property type="nucleotide sequence ID" value="XM_014052962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3L7"/>
    <dbReference type="GeneID" id="106538472"/>
    <dbReference type="KEGG" id="tsr:106538472"/>
    <dbReference type="CTD" id="56848"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008481">
        <property type="term" value="F:sphingosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006669">
        <property type="term" value="P:sphinganine-1-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000005">
        <property type="entry name" value="Sphingosine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF40">
        <property type="entry name" value="SPHINGOSINE KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="9">
        <location>
            <begin position="149"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013908437.1"/>
        </source>
    </evidence>
    <sequence length="599" mass="66188" checksum="759D656F66057ADA" modified="2020-10-07" version="1">MNLVLKPCREGSEAILHGEFGAYPPKGIRYALSLTRTELHIQRLVPKPDTDQRTVIRLIDVVGCHTLRSHTVANQSAFFCIYAYPLKKKKVAMGSGRARQRIAKTFQVDGAERYKDNQALAEKWAAAIKCLVLGIPISSETEISSNLLPRSRRLLLLLNPFGGKGNALQWCQNHILPMITEADVSFNLIQTERQNHARELVQSISLDEWDGIVVISGDGLLHEVINGLMERPDWEEAVKMPLGILPCGSGNAVAAAINCSAGFEQVLGQELLTNCTLLLCHGAVSPLDLVSITTTSGTRVFSFLSVAWGFISDVDIESEKYRHMGPARFTLGTMVRLASLNIYRGRLSYLPALGCATHRPILRSITVAANGSLPFQRLPLHRTVSDMGLCEERQAFCYREMESAEEPTPSPSSPPSSSFQASNFSFESLSEVTVDKESQHADLPKVLPNQLPSARQMHGPLDDFLAPLEQPVPKSWVTVEDDFVLVLAIYQTHLGADLFTMPTAGFNDGLIHLCFVRAGVSRATMLRLFLAMEKGTHIDHENPYFTHLPVRAFRIEPLTHKGIITIDGERVEYGPIQGQIHHGIANLVTGINRSKVTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3N6</accession>
    <name>A0A6I9X3N6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106538488</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013908457.1">
        <property type="nucleotide sequence ID" value="XM_014052982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3N6"/>
    <dbReference type="GeneID" id="106538488"/>
    <dbReference type="KEGG" id="tsr:106538488"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01863">
        <property type="entry name" value="Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000430">
        <property type="entry name" value="Probable Ras-related protein Rab-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013908457.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="26030" checksum="6372325AC90BF5B7" modified="2020-10-07" version="1">MLRWPSCFHSAKFPVHLVIYLTMARKDTVPLPTLKLLLIGDSAVGKSSLLLRFAEDTFEPYLSPTIGVDFKVKKMVVGNFPIQLAIWDTAGQERFRTLTPSYYRGAQGIILVYDVTRRETFLGLESWLQELEIYTKKNVVKMLVGNKIDETNREVEKREGLLFAQKHSMLFIEASAKMKAGVQSAFEEVVMRILQTPELWNIRREGVKLAESRSFREADACNSYCSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4M2</accession>
    <name>A0A6I9X4M2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycerol kinase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GK5</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910309.1">
        <property type="nucleotide sequence ID" value="XM_014054834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4M2"/>
    <dbReference type="GeneID" id="106539912"/>
    <dbReference type="KEGG" id="tsr:106539912"/>
    <dbReference type="CTD" id="256356"/>
    <dbReference type="OrthoDB" id="6278781at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF68">
        <property type="entry name" value="GLYCEROL KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013910309.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15538" checksum="3D27511F0F9D9085" modified="2020-10-07" version="1">MGITPTTTKKHLVRAVLESIAFRNKQLHDIITTEVNIPSMSIRADGGVSKNSFVMQMTSDLINKSINKPDSTDMSCLGAAFLAGLAIGYWTDKEHLKTLRRTDMIFKPQREPKEYEPAMSNWIKAVCRSLSWYSQASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5H6</accession>
    <name>A0A6I9X5H6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PTPN22</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013909521.1">
        <property type="nucleotide sequence ID" value="XM_014054046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5H6"/>
    <dbReference type="GeneID" id="106539281"/>
    <dbReference type="KEGG" id="tsr:106539281"/>
    <dbReference type="CTD" id="26191"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050868">
        <property type="term" value="P:negative regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047170">
        <property type="entry name" value="PTN12/18/22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45983">
        <property type="entry name" value="TYROSINE PHOSPHATSE N18, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45983:SF1">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="204"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="675"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909521.1"/>
        </source>
    </evidence>
    <sequence length="710" mass="81127" checksum="30BE7A60FCE45417" modified="2020-10-07" version="1">MDQREIIMKNLKRIRNRNLNKEEFTSEYLKLKRQSATYRAEKIYPTTVSERPENSSKNRYKDILAYDHTRVKLSFITSDKDSDYINANFIKGVYDPRTYIATQGPLPTTVIDFWRMIWEYKILIIVMACMEFEMGKKKCEHYWVDVDESPLQLGPFFIICEAEEKRQDYVIRKLKVKVNDNEFRSVYHFHYKNWPDHDIPSSINSIFQFINEMHSYQANHDVPICVHCSAGCGRTGVICAIDYTWTLLRDNKLKRQSATYRAEKIYPTTVSERPENSSKNRYKDILAWASWGYIKEAGVDAFRKRERKGQYKLVYDAVIELFKRQIEAFSSHSDLVVIETGINHSVANIPQDPDRQRSCIKQSNSSVQEHQEKSRCPGCATQSLNILDRNRDSASLVRPALSVGALNPTAGRNTGWDSWLAKQPLHKYHSLDFSYCFSTDPLLPVKMDDKTGKGLDTKVHPIQTESTPFQSVSQKTLQPLAKMDSESGHCGGFQLDSNLSNNLSNNAPGYKLKDLCHQHCVWSSEDPYFSSLSSEEPCSPAHRESALSPLRTVPSSSAVAFTEQAMESSEATYSLFNPPAQDTCPLDAVCHSSAQPDDDIPPPLPERTPESFIVLGEENEFQQLDKPFLAVSDHQLPSKNDKTKISAEWSSMSQLRFSDDSVRLRPNKSIKLQRPRSELLWNRSSSPPTPPLPERTPESFLLADEDPCPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5S5</accession>
    <name>A0A6I9X5S5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GATOR2 complex protein WDR59</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106539032</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway. The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex. GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1. In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation. In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the GATOR2 subcomplex, composed of MIOS, SEC13, SEH1L, WDR24 and WDR59. The GATOR2 complex interacts with CASTOR1 and CASTOR2; the interaction is negatively regulated by arginine. The GATOR2 complex interacts with SESN1, SESN2 and SESN3; the interaction is negatively regulated by amino acids. Interacts with DDB1-CUL4A/B E3 ligase complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat WDR59 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909177.1">
        <property type="nucleotide sequence ID" value="XM_014053702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5S5"/>
    <dbReference type="GeneID" id="106539032"/>
    <dbReference type="KEGG" id="tsr:106539032"/>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000266">
        <property type="entry name" value="WD repeat-containing protein 59 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 13" id="KW-0853">WD repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="101"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="187"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="14">
        <location>
            <begin position="393"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013909177.1"/>
        </source>
    </evidence>
    <sequence length="569" mass="64651" checksum="BDD8E37E2EF63D77" modified="2020-10-07" version="1">MAARWSSENVVVEFRDSQATAMSVDCLGQHAVLSGRRFLYIVNLDAPADGHRKISRQSKWDIGAVQWNPHESHAHFFAASSNQRVDLYKWKDGNGEIGTSLQGHTRVISDLDWAVFESDLLVTSSVDTYIYIWDIKDTRKPTVSLSAVAGASQVKWNKKNANCLATSHDGNVRIWDKRKPSTAMEYLAAHLSKIHGLDWHPDNENILATSSQDNSVRFWDYRQPRKYLNILACQVPVWKARYTPFSNGLVTVMVPQLRRENSLLLWDICDLNTPVHTFVGHDDVVLEFQWRKQKEGSKDYQLVTWSRDQTLRMWRVDCQLQRLCSSDLLEGVEELIDGISLLPEADKALPVPETEPQQNFTHGEEEIFKEDSQKSFLVGKKLDQLGLPQTLQQEFSLINVQIRNVNMEMDAVNRNCTVSVHCSNHRVKMLVMFPAQYPNNAAPSFQFVNQTTIPSTMKAKLLKILKDTSMQKVKRNQCCLEPCLRQLVSCLESVVEDSSSGDPYAHPNSVTPPLPTFPRVSNAYGSYQDSNIPFPRTSGARFCGAGYLVYFTRPMTMHRTVSPTEPTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6H6</accession>
    <name>A0A6I9X6H6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Importin-11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Ran-binding protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">IPO11</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with UBE2E3 and RPL12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909442.1">
        <property type="nucleotide sequence ID" value="XM_014053967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6H6"/>
    <dbReference type="GeneID" id="106539224"/>
    <dbReference type="KEGG" id="tsr:106539224"/>
    <dbReference type="CTD" id="51194"/>
    <dbReference type="OrthoDB" id="361693at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000116">
        <property type="entry name" value="importin-11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF7">
        <property type="entry name" value="IMPORTIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="14">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013909442.1"/>
        </source>
    </evidence>
    <sequence length="974" mass="112698" checksum="4F6FCFDFD463AD55" modified="2020-10-07" version="1">MDLHSASNIVLQVLTQATSQDTSVLKPAEEQLKQWETQPGFYSVLLNIFTNHSLDINVRWLAVLYFKNGIDRYWRRMAMHALSEEEKSTLRAGLIANFNEPVNQIATQISVLIAKVARLDCPRQWPELIPTLVESVKIQDDLRQHRALLTFYHVTKTLASKRLAADRKLFYDLASGIYSFTCSLWNHHTDTFLQQICVRDETMSNSLERTLLSLKVLRKLTVHGFVEPHKNIEVMGFLHAVFVRLKQFLECSKNIETDNACRDRLEKTIILFTKVLLDFLDQHPFSFTSLIQRSLEFSVSYVFTEAGEGVVFERFIVQCMNLIKMIVKNYAYKPSKNIEESSPETLEAHKIKASFFTYPTLMEICRRLVTQYFLLTEEELTMWEEDPEGFIVEETGGDSWKYSIRPCTEVLFIDIFHEFNQTLTPVLLEMVHSLQGPTNMDDTHALLIKDAVYNAVGLAAYELFDSVDFDQWFKTQLLAELQVIHNRYKPIRRRVIWLIGQWISVKFKPDLRPMLYDAICNLLQDEDLVVRIETATTLKLAVDDFEFRTDQFLPYLESMFTLLFKLLQEVRECDTKMHVLHVLSCVIERVNIQIRPYVACLVQYLPLLWKQSEEHNMLRCAILTTLIHLIQGLGAESKNLYTFLLPIIQLSTDVSQPPHVYLLEDGLELWLVTLENSPCLTPELLRIFQNMSALLELSSENLRICFKIINAYLFLSSSEFLQIYSSDLCRSFCEILKDVTTEGQVQVLKVVENVFKVNPVLGPEMFQPLLPSVIRGIIQGERYPVEMSTYLGVIGRVLLQNKSFFSLLLNQMACEFNQEPDHLLGHIIEMWVDRMDNITQPERRKLSALALLSLLPSDNSIVQDKFCGIINISVEGLHDVMTEDIETRTFKDCMLMSSFEEPKVIEDEEPPTEQDKRKKMLALKDPVHSVSLQQFVYEKLKAQQELLGEQSFQALMETVDTEIVAQLQDFLQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7B2</accession>
    <name>A0A6I9X7B2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adaptin ear-binding coat-associated protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106539782</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NECAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910146.1">
        <property type="nucleotide sequence ID" value="XM_014054671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7B2"/>
    <dbReference type="GeneID" id="106539782"/>
    <dbReference type="KEGG" id="tsr:106539782"/>
    <dbReference type="OrthoDB" id="10265489at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012466">
        <property type="entry name" value="NECAP_PHear"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847:SF16">
        <property type="entry name" value="ADAPTIN EAR-BINDING COAT-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847">
        <property type="entry name" value="ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07933">
        <property type="entry name" value="DUF1681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NECAP PHear" evidence="5">
        <location>
            <begin position="86"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013910146.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="26910" checksum="556068F5648E8A76" modified="2020-10-07" version="1">MASGGERIYRWRLNSRHPLPCGPVASHLVSSLCREGPLGGSCCKLELRVPCFGYDSAFLRSLFQASCWANLQPATSAAFLSLVFLTGRRAFIGIGFVDRGDAFDFNVALQDHFKWVKQQTELAKEAQNPTQVPKLDLGFKDGQTITLSIGNMKKKDGSLGSKPRTADNAGLPLLPPPPGGKTPFLPTPEEQLPLPPLGTSVDAVQTDVAESRPPQPPSSSREASVDIWGDFTTASGSGSQPQQNSGWVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7I5</accession>
    <name>A0A6I9X7I5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ankyrin repeat and fibronectin type-III domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ANKFN1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909797.1">
        <property type="nucleotide sequence ID" value="XM_014054322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7I5"/>
    <dbReference type="GeneID" id="106539524"/>
    <dbReference type="KEGG" id="tsr:106539524"/>
    <dbReference type="CTD" id="162282"/>
    <dbReference type="OrthoDB" id="2428204at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061172">
        <property type="term" value="P:regulation of establishment of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039269">
        <property type="entry name" value="ANKFN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437:SF3">
        <property type="entry name" value="ANKYRIN REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437">
        <property type="entry name" value="WIDE AWAKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013909797.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="59313" checksum="1215207318032706" modified="2020-10-07" version="1">MLVTNEDHIPIVEIDDSHASSIMQDFLWFTKDILSYHRRSQQRLAPGLYLGYLKLCSSVDQIKVLVLQKLPNILCHVKIRDNCNVSREEWEWIQRLSGSESMENMDHSAECPTRLFFELQMAMRALLKQINLPLQQAKLFRLYTQEVLELGHNVSFLLLLPASDDVCTAPGQNNPYTLHTGFLNLPLQMFELVHFCSYKEKFISLYCRLSAVIELDFLSTQQSLREAISDSEVAAAKLRHQQVLDYIQRNIFSNNGNAVAQQLRDSQPYSDEEGCSEVFLPTDSDYDSSDALSPRELDLVYSSSQDISHQAVSCLSGSAPDVLQVHDIKPCLTPKQNPRGSSVLETEVDQSTENMQSLSIGRLSPKNPEKNSGSKQSLSFLGKKKHGKHQHYNYFSRHHRWLRVHSETQSISLSEGLYTPHLTQAMDYSQEPTPGEQISISIDCPRSTFLPHASSLPEEGKNKYQELRPNIRRIYVEPYKTILSSEGSKQSGDHPDLHSPLGQGRIAEDQSCSNIPEVFSSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X871</accession>
    <name>A0A6I9X871_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase TRIM63</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TRIM63</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909992.1">
        <property type="nucleotide sequence ID" value="XM_014054517.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106539674"/>
    <dbReference type="KEGG" id="tsr:106539674"/>
    <dbReference type="CTD" id="84676"/>
    <dbReference type="OrthoDB" id="5351233at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19831">
        <property type="entry name" value="Bbox2_MuRF1_C-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000014">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase MID2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000140">
        <property type="entry name" value="Tripartite motif containing 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="23"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="COS" evidence="12">
        <location>
            <begin position="257"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="312"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="200"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909992.1"/>
        </source>
    </evidence>
    <sequence length="342" mass="38811" checksum="0EF2917A02CAF6E7" modified="2020-10-07" version="1">MDYQSSIFQDNNPMENLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDIFQASNPYWQTSRGTTISGGRFKCPSCRHEVILDRHGVYGLQRNLLVENIIDIYKQEYSSRPLKKGNHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGAHKDCEVAPLQSVFQGQKVXANEICSEGTNSTSFILLSLXQENSQLAKQSLCEKFEALIAVLEEKRTNLLQRITKEQEEKAGFMQNLIQQYKEQLETSSKLIESAIQSMEEEGGAAFLMTAKQLIKTIVDASKGGQLEKIETGFENMDYFTVDLESITEALRSIDFEADEDDDELNEEDETEEEQPVGQTDGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XA93</accession>
    <name>A0A6I9XA93_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Selenide, water dikinase 1</fullName>
            <ecNumber evidence="5">2.7.9.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Selenium donor protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Selenophosphate synthase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC106540591</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Synthesizes selenophosphate from selenide and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hydrogenselenide + ATP + H2O = selenophosphate + AMP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18737"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:29317"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the selenophosphate synthase 1 family. Class II subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.3" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013911241.1">
        <property type="nucleotide sequence ID" value="XM_014055766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XA93"/>
    <dbReference type="GeneID" id="106540591"/>
    <dbReference type="KEGG" id="tsr:106540591"/>
    <dbReference type="OrthoDB" id="409395at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000006">
        <property type="entry name" value="Selenide water dikinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000003">
        <property type="entry name" value="Selenide, water dikinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="SELENIDE, WATER DIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256:SF1">
        <property type="entry name" value="SELENIDE, WATER DIKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0711">Selenium</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="22">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="23">
        <location>
            <begin position="126"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013911241.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="35765" checksum="64392F1AD710A69C" modified="2020-10-07" version="1">MDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQKMTEEEREKIMPLIIKGFRDAAEDGGTSVTGGQTVLNPWIIVGGVATVVCQPNEFIMPDSAVPGDVLVLTKPLGTQVAVNAHQWLDNPERWNKIKLVVSKEDVELAYQEAMFSMAMLNRTAASLMHTFNAHAATDITGFGILGHAQNLAKQQRSEVSFVIHNLPIIAKMAAISKACGNRSRLLQGTSPETSGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNQTARIIDKPRIIEVTPRGASASPQDNNSSASPEVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAF7</accession>
    <name>A0A6I9XAF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60 kDa heat shock protein, mitochondrial</fullName>
            <ecNumber evidence="3">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Chaperonin 60</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Heat shock protein 60</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HSPD1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013908228.1">
        <property type="nucleotide sequence ID" value="XM_014052753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XAF7"/>
    <dbReference type="GeneID" id="106538306"/>
    <dbReference type="KEGG" id="tsr:106538306"/>
    <dbReference type="CTD" id="3329"/>
    <dbReference type="OrthoDB" id="1733909at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000019">
        <property type="entry name" value="60 kDa heat shock mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000011">
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        <property type="entry name" value="Chaperonin 60 subunit alpha 2 chloroplastic"/>
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        <property type="entry name" value="Heat shock protein 60"/>
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        <property type="entry name" value="GroEL"/>
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        <property type="entry name" value="GroEL-like equatorial domain"/>
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        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
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        <property type="entry name" value="CH60"/>
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        <property type="entry name" value="PRK12851.1"/>
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    </dbReference>
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        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="entry name" value="Cpn60_TCP1"/>
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        <property type="entry name" value="CHAPERONIN60"/>
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        <property type="entry name" value="GroEL apical domain-like"/>
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        <property type="entry name" value="CHAPERONINS_CPN60"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0346">Stress response</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012198"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00029756"/>
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            <dbReference type="ARBA" id="ARBA00030005"/>
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            <dbReference type="ARBA" id="ARBA00037436"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013908228.1"/>
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    </evidence>
    <sequence length="572" mass="61119" checksum="E59936E4AD72B476" modified="2020-10-07" version="1">MLRLPSVLRQLRPVSRALAPHLTRAYAKDVKFGAEARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKAIDLKDKYKNIGARLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVEAVINELKKLSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTAKGQKCEFQDAYLLISEKKISTVQSIVPALEIANNHRKPLVIIAEDLDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLSINLEDVQPHDFGKVGEVIVTKDDCLLLKGKGDKTQIEKRIQEIIEQLETTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDIEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDALTPVNEDQKTGIEIIRRTLKIPAMTIAKNAGVEGSLIVEKIMQSSPEIGYDAMLGDFVNMVEKGIIDPTKVVRTALMDAAGVASLLSTAEAVVTEIPKEEKDAAAAMGGMGGGMGGGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAL6</accession>
    <name>A0A6I9XAL6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">WNK4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013910837.1">
        <property type="nucleotide sequence ID" value="XM_014055362.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540294"/>
    <dbReference type="KEGG" id="tsr:106540294"/>
    <dbReference type="CTD" id="65266"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14033">
        <property type="entry name" value="STKc_WNK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase WNK1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein kinase WNK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000494">
        <property type="entry name" value="serine/threonine-protein kinase WNK2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056865">
        <property type="entry name" value="CCTL2_WNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24889">
        <property type="entry name" value="CCTL2_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="158"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="534"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="658"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1020"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1208"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="670"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1033"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1093"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1214"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1242"/>
            <end position="1252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910837.1"/>
        </source>
    </evidence>
    <sequence length="1282" mass="139800" checksum="4F5DED5B163E78E9" modified="2020-10-07" version="1">MLMLRASHSGPGAGALTPQTDRMDQTSCGMDEDAGQVEGPRTLGVSGRRASAYKISRRSSTELGLRSFDGEDGAPAVAAGPDPMDLEGMEPLATVLESSKSRGLLEGSASSESSPEVAPSNPLLCLTESRQDREHWEQEENEEVETRAVATSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLSKMERQRFSEEVEMLKGLQHPNIVRFYDSWKSSIKGQVCIVLVTELMTSGTLKTYLKRFKEMKMKVLQRWSRQILRGLHFLHTRSPPIIHRDLKCDNVFITGPTGSVKIGDLGLATLKCASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGMKPNSFHKVKVPELKEIIEGCIRMNKNERYTIQDLLDHSFFHEDTGVHVELAEEDDGIKTTLKLWLRMDDTKKLHGKYKDNNALEFYFELYKDVAEEVAQEMVVLGFVCEADYKPVAKAVRERVLAIKRQREKTKQAQEQQKRQEQSLAMTPDILHLLDLDSPSPLSPVQTSNPTTPGSADSALGSTFPTEPEEPEVDQHQPFQFHPNYSSTTSDCETDGYLSSSGFVDSPEKLKSHSSPITVSRHSFASTIAVSLDPVSISDISSPVDSYTSDVASGLSDGYEGLSASEDRSKPLTKRGSGRLARRRARSRLRITNMPNQNDQVVECQLQTYNNKMVTFKFDLDGDNPEEIAAAMVAKEFILKSEQEQFIQGIHDIILRVEALLQKDKLNKEQGALGKPDVQCKSPTSATNAPSAELPLQDLSCSISSSSSLSDFGCLSSGQPVSSPIHTSVVDAISENDPFSPTIPPTTTQNLGQPLLSTTSAGITSTPGQTWPTYHMYPGTPTTPSYFVPIPQPISLPNSVLPTLPLANSTAPSIGTTVHAPPIPMWSTSTSPLFSFANVFSLAMMSMAQSFLPAVSVGTAQTGPLYTSPLPQPPVLCPPSPTTVRFIYPELGSSPKPAHFVNGMLGSVVSPGCKPTPVPVVAGNSENHPVPPVSSWSGTELTVSDQSTSNGSPAEHTTVSEGETKPQILGRFQVTPTRNIPSESPPKDNGPNISSTSESDSGSPGETPVQEGEEGTTTVAEGEGEGELPHGNTVWMNFMHIPSCPSSDDSDNTEDDDVWVELQALRQKHLSEVEKLQAQQKQEIEELYARLGKVPPAGIVSPATILSSRQRRLSKGSSNTLRRSSLQRPDTTQPQGIMRRNSVGGSNLASQEQRQSKGVKFASDIGCLVRNGLGMQLAGNSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCD0</accession>
    <name>A0A6I9XCD0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106538742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908818.1">
        <property type="nucleotide sequence ID" value="XM_014053343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCD0"/>
    <dbReference type="GeneID" id="106538742"/>
    <dbReference type="KEGG" id="tsr:106538742"/>
    <dbReference type="OrthoDB" id="344165at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013908818.1"/>
        </source>
    </evidence>
    <sequence length="81" mass="9280" checksum="1587BEF39568AB16" modified="2020-10-07" version="1">MLSFTCVGGNESRYSWQQFGLLLCDTKQEKCMDKPGPKLDSRAETCFVNCVERFIDTSQFILNRLEQTQKSKSPFSESLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDE2</accession>
    <name>A0A6I9XDE2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat-containing protein 59-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106541077</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911882.1">
        <property type="nucleotide sequence ID" value="XM_014056407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDE2"/>
    <dbReference type="GeneID" id="106541077"/>
    <dbReference type="KEGG" id="tsr:106541077"/>
    <dbReference type="OrthoDB" id="9315669at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16692">
        <property type="entry name" value="mRING-H2-C3H3C2_WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039456">
        <property type="entry name" value="WDR59_mRING-H2-C3H3C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR59/RTC1-like RING zinc finger" evidence="4">
        <location>
            <begin position="172"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013911882.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="24965" checksum="157DC01D9EE3CC4D" modified="2020-10-07" version="1">MLCSVFETQSRPQGIPNPTGLAPQHPSSHSRYPSFTSSGSCSSMSDPGLSTSSWNTASKDMEHTLGWAESSPDDFRLGNLTYADPRDRERDQHDRNKRLLDPANTQQFDDFKKCYGEILYRWGLREKRAEVLKFVSCPPEPHKGIEFGVFCSYCRSEVRGIQCAICKGFAFQCAICHVAVRGSSNFCLTCGHGGHTSHMMEWFRTQEVCPTGCGCHCLLESTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDQ2</accession>
    <name>A0A6I9XDQ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PHD finger protein 21B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106539109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013909268.1">
        <property type="nucleotide sequence ID" value="XM_014053793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDQ2"/>
    <dbReference type="GeneID" id="106539109"/>
    <dbReference type="KEGG" id="tsr:106539109"/>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102:SF18">
        <property type="entry name" value="PHD FINGER PROTEIN 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013909268.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="34916" checksum="2A781F8FE6850427" modified="2020-10-07" version="1">MSYSQKRKAKLGDEIRKRIPMQLDGEIMYTIQVRLGENLDRRPLFVLIFQRSNGRLLRGNKLVAFSEHALGTITSVPIKVPQVSSLQRLAGQKPAVLPQVRPKTLIPDSLPISPCRDQPSKQPPTFQKATVVSIKNPAPALPTANNTVSHVQTSSNQSQSVAETAALSSSLNSAGVAYAIISTSPNNAMPINTSAAVSVVNDSIKVQPLLISADSKVIIIQPKVPTQTENKVEIKKPAEESTQGPATKKKKDENPEKIAFMVALGLVTTEYLEEIQSKRQERKRRSTANPAYSGLLETERKRLASNYLNNPFFLSTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XE24</accession>
    <name>A0A6I9XE24_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">F-box/WD repeat-containing protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FBXW12</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912122.1">
        <property type="nucleotide sequence ID" value="XM_014056647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XE24"/>
    <dbReference type="GeneID" id="106541264"/>
    <dbReference type="KEGG" id="tsr:106541264"/>
    <dbReference type="CTD" id="285231"/>
    <dbReference type="OrthoDB" id="63265at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052121">
        <property type="entry name" value="F-box_SCF_Substrate_Recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46550:SF2">
        <property type="entry name" value="EXPRESSED SEQUENCE C85627-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46550">
        <property type="entry name" value="F-BOX ONLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912122.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="53764" checksum="C8E1D95C860804E9" modified="2020-10-07" version="1">MSAESLTLDCTIHLFSYLEVPDLLRAAQVNKAWNEAAETTFLWRNMCLKRWAFCNISVIPGIHSWKKYYLHRSNLESKMTSGQGSVDYTCKAIRGHNGVIHEMAYLSENQHMFATGKVNSTICSVSSDGTVKAWNVQEGKQIWSSPQQEFPLVNIITFPAFNLAATADTQGTIKLWHGTTGEELSTFSVSSTSCCMVAYTIKNNPFLMVGTEEGSLHTLAATDLSQILYKKMFQNCSTQLSLCSPDGQWILVCPSNSAFSPKVFNTYYATQPEDDDLILSSPLPITNHCQVTCWLPTESARIAILHKNEMFHIDSFDLAIEKSKYKTTVTAKQVASFTLPAQQQMTNMKGFGKQTLLIAAGPELTVYSLSGTVLMAFKDHHKIITSIWVDPFHVVTSSMDLSLRVYYWKNNKSSLLTSCYHLLGGSHRWSSGFRSVACNDVSIVGIVAGIDGRHFKSIFLSSVRVPCTCFTLNIFHQIVQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFL6</accession>
    <name>A0A6I9XFL6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="18">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">B4GALT5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17950"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:31496"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Golgi apparatus membrane</location>
            <topology evidence="18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_013909848.1">
        <property type="nucleotide sequence ID" value="XM_014054373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFL6"/>
    <dbReference type="GeneID" id="106539563"/>
    <dbReference type="KEGG" id="tsr:106539563"/>
    <dbReference type="CTD" id="9334"/>
    <dbReference type="OrthoDB" id="10038994at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008489">
        <property type="term" value="F:UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009101">
        <property type="term" value="P:glycoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000037">
        <property type="entry name" value="Beta-1,4-galactosyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF45">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="70"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="209"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013909848.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="40213" checksum="50223A6E81AA2528" modified="2020-10-07" version="1">MMQAQGIMIRENMKTIGAQVYEQVVRSAYAKRNSTGNDSDYPLDLNHSENFLQTTTFLPEDFTYFPNHICPERLPSMKGPIDVNMSEISMDEIHQFFSQDPTIKLGGHWKPSDCLPRWKVAILIPFRNRFEHLPVLFKHLIPLLQRQRLQFAFYVIEQAGNQPFNRAMLFNIGFREAMKDLDWDCLIFHDVDHIPENDRNYYGCGQMPRHFATKLDKYMYILPYAEFFGGVSGLTVKQFKKINGFANAFWGWGGEDDDLWNRVHYAGYLVTRPEGDTGRYKSIPHHHRGEVQFLGRYALLRKSKERQELDGLNNLNYFPNITYDSLYKNITVNLSPELALVSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG30</accession>
    <name>A0A6I9XG30_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein EFR3 homolog B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">EFR3B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane. The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis. In the complex, EFR3B probably acts as the membrane-anchoring component. Also involved in responsiveness to G-protein-coupled receptors; it is however unclear whether this role is direct or indirect.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and HYCC (HYCC1 or HYCC2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EFR3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913116.1">
        <property type="nucleotide sequence ID" value="XM_014057641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG30"/>
    <dbReference type="GeneID" id="106542038"/>
    <dbReference type="KEGG" id="tsr:106542038"/>
    <dbReference type="CTD" id="22979"/>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000139">
        <property type="entry name" value="protein EFR3 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049152">
        <property type="entry name" value="EFR3-like_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051851">
        <property type="entry name" value="EFR3_Homologs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12444:SF4">
        <property type="entry name" value="PROTEIN EFR3 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12444">
        <property type="entry name" value="PROTEIN EFR3 HOMOLOG CMP44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21052">
        <property type="entry name" value="EFR3_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913116.1"/>
        </source>
    </evidence>
    <sequence length="782" mass="88651" checksum="959C93740B4E0F65" modified="2020-10-07" version="1">MEKLTFYALSAPEKLDRIGAYLSEKLIRDVGRHRYGYVCIAMEALDQLLMACHCQSINLFVESFLKMVAKLLESEKPDLQILGTNSFVKFANIEEDTPSYHRSYDFFVSRFSEMCHSSHDDMEIRTKIRMSGIKGLQGVVRKTVNDELQANIWDPQHMDKIVPSLLFNLQHAEEAESRSPSPLQTTEKEKENPAELAERCLRELLGRAAYGNIKNAIKPVLIHLDNHSLWEPNTFAIRCFKIIMYSIQPQHSHLVIQQLLSHLDANSKSAATVRAGIVEVLSEAAIIAASGSVGPTVLEVFNTLLRQLRLSIDYALTGSYDCVIAGGTKIIKEHEERMFQEAIIKTTGSFASTLPTYQRSEVMLFIMSKVPLPSLHHPVDMGKAGENRNRLTQIMLLKSLLQVSTGFQCSNMLTALPSSFLDRLLSATLMEDAEIRLFVLQILISFIDRHDNKHKFQTISTISDISILKLKVDKCSRQDTVFMKKHSQQLYRHMYFTCKEDNNGHTHYEAVYSLLALISIELANEEVVVDLIRLVLAIQELAQVNEDNLPLYSRCALYALGAAYLNLISQLTTVPAFCQHIHEVIEMRKKEAPYLLPEGVFVEKPRLSRNLDHLGPEILFRQSKISEVLGGSGYNSDRLSTPYIPQLTDEDRLSKRRSIGETISLQVEVESRNSPEKEERAPAEEITYETLKKAIVDSVAVEEQERERRRQVVEKFQKAPFEEIAAHCGARATLLQNKLNQIFEITIRPPPSPSGTITAAYGQPQNHSIPVYEMKFPDLCVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGK5</accession>
    <name>A0A6I9XGK5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Origin recognition complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ORC1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">ORC is composed of six subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ORC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914819.1">
        <property type="nucleotide sequence ID" value="XM_014059344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGK5"/>
    <dbReference type="GeneID" id="106543345"/>
    <dbReference type="KEGG" id="tsr:106543345"/>
    <dbReference type="CTD" id="4998"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000062">
        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000199">
        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054425">
        <property type="entry name" value="Cdc6_ORC1-like_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF23">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22606">
        <property type="entry name" value="Cdc6-ORC-like_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAH" evidence="14">
        <location>
            <begin position="58"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="399"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013914819.1"/>
        </source>
    </evidence>
    <sequence length="870" mass="98458" checksum="64EA7DA1F2A43214" modified="2020-10-07" version="1">MNCSPSWRKSYPRKDTLNTRSRGKRIYSWIGKAISTDKTLQISHYRGISINKKDISDICIFPGEFIFIEEEKYDDFFVARLLQLYEDGAQKKHAIIEWFSRFEEIPFGLRNSLGQKNSQEIFLNEKTNWDTDIFVSSIIGKIMVIPLSPCEPLPIEVKDKQVMFVRKSWDGKKLKPLRPNLLAELKNSVKLKNDIHFATECTRTPSRSLEEATENVTWSNKSSAEVKLRHSAAKSSLFKQRYSPKLVNGDIAGAKKRLQLNTPTKSTNMLAVQQDVLELLDCGLTETPQRSPLKRKVTFTGIKGSPKKISCVSDEEDLLSDIKPLDEHPERTTTNLLTLCQRIQDSSLKISGCAVTQSGKGLDEECELETRNPVMTPRSRRKCAQVTAARIAKQLRILNPARDEGKGKKCLSPLESSDSDSSDEEDNKKLHTPKRQTKFTPPKFSKNQSMGTPAKDPRNSSKPSTPKTPRNATPRIRSRNQTAQKPVNVLEEARMRLHVSAVPDALPCREKEFQDICNFIKSKLLDGTGGCMYISGVPGTGKTATVHEAIHYLQQAAENDEIPVFQFIEINGMKLTDPHQAYVLILKLLTGQKTTANHAAELLKKMFCTPGSKRKTLVLIVDELDLLWTRKQNVMYNLFDWPTQKDAKLIVLAIANTMDLPERMMMKRVASRLGLTRMSFQPYTYKQLQQIVSSRINQLKAFEEDAIQFISRKVAALSGDARRCLDICRRSTEICELNCKRGVSGLVNMAHVMQAMDEMFSSPYINAIRNASLQEQIFLKATIAEFHRLGLEEATIQQIFHQHIGLCRLEGLPTPTVSDIMAISSRLGACKLLLAESHAKYLSMRIRLNVSQNDVMYALKQDQNAKNTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH14</accession>
    <name>A0A6I9XH14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Eukaryotic peptide chain release factor GTP-binding subunit ERF3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106539913</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910313.1">
        <property type="nucleotide sequence ID" value="XM_014054838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH14"/>
    <dbReference type="GeneID" id="106539913"/>
    <dbReference type="KEGG" id="tsr:106539913"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000017">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000024">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000270">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
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        <property type="entry name" value="TRANSLATION FACTOR"/>
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    </dbReference>
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        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
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        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="Translation proteins"/>
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    </dbReference>
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        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="209"/>
            <end position="435"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="55"/>
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            <end position="127"/>
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            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
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        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
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        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
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            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00007249"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023161"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00049117"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013910313.1"/>
        </source>
    </evidence>
    <sequence length="636" mass="69300" checksum="45B16B64E210605F" modified="2020-10-07" version="1">MEANGSGSSSDSAPDCWDQADLEPGNAASAAAGDEEAAAAPGSAPLESEESEEEQLLGAAFSRHLNVNAKPFVPNVHAAEFVPAFLRGGPGQPATPTAPSPPPGLPTPTAPSLTHGASAESSQEDLLSCEGSNVAIGMEISEPVVENGETEMSPEESWDHKEEIIESELASGPSGDAGPIEESTQEMTEEEEEMPIPKSIVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEPSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSMCKGQQLVMMPNKHNVEVLGILSDDVETESVSPGENLKIRLKGIEEEEILPGFILCDLSNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHF8</accession>
    <name>A0A6I9XHF8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mesoderm development candidate 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MESDC1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913531.1">
        <property type="nucleotide sequence ID" value="XM_014058056.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106542353"/>
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    <dbReference type="CTD" id="59274"/>
    <dbReference type="OrthoDB" id="10009851at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Talin_IBS2B"/>
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    <dbReference type="InterPro" id="IPR042799">
        <property type="entry name" value="TLNRD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47133">
        <property type="entry name" value="TALIN ROD DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47133:SF1">
        <property type="entry name" value="TALIN ROD DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21896">
        <property type="entry name" value="Talin_IBS2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Talin IBS2B" evidence="2">
        <location>
            <begin position="168"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913531.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="37666" checksum="80753A7E551F487D" modified="2020-10-07" version="1">MASGGSGKSTTETSTPSGSSLPRRKLVATCDLCKIKMQLVADLLLLTSESRPVHTESLSVFGESFEKCRDTIIARTKGLSILTHDVQSQLNMGRFAEVGDILGEMGDLVVSLTECSAHAAYLAAVETPGAQPALPGLVDRYKVTRCKHEVEHGCGILKTTPLVDLSPQLLLEVSQNMSKNLKFLTDACVLASEKSKDRFAKEQFKLSVKCMSTSASALLACVREVKTSPSELTRNRCVLFSGPLVQSVCALVGFATEPQFLGRAATINPEGKAVQTAILGGAMSVVSACVLLTQCLRDIAQHNDSSTKMTEYRERLRNSACAVSDGCNLLSQALRERSSPRTLPPANANSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJE3</accession>
    <name>A0A6I9XJE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleoporin NDC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Transmembrane protein 48</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NDC1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the nuclear pore complex (NPC), which plays a key role in de novo assembly and insertion of NPC in the nuclear envelope. Required for NPC and nuclear envelope assembly, possibly by forming a link between the nuclear envelope membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911028.1">
        <property type="nucleotide sequence ID" value="XM_014055553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJE3"/>
    <dbReference type="GeneID" id="106540454"/>
    <dbReference type="KEGG" id="tsr:106540454"/>
    <dbReference type="CTD" id="55706"/>
    <dbReference type="OrthoDB" id="67850at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019049">
        <property type="entry name" value="Nucleoporin_prot_Ndc1/Nup"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269:SF6">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013911028.1"/>
        </source>
    </evidence>
    <sequence length="673" mass="76096" checksum="7C55AFA2E5FA5E4C" modified="2020-10-07" version="1">MGDVSTREQLSILRWRAAASIAWSVLLLPLCVAVFISITTIDLFHPVQWITDSFNDLYTSYVIFCILLLSVVILVINVFNVQFHAVVPSIHCSRLALISKIIHPQQVIHSVAHAVMGMLVAWCAAVMTKGKFLFLSMPCTATTTESADDATLHTCLNEYHLFLLLLGAFMGYSYSLRYLVNNLNYLPFPVIQQYKYLRFRRSLPLLAKHSCVESLYMVRNFCAVYYFFGYIPRTWIMTTMNLRADSNLPLLDTVAGLLDLSLLYHSWLCGLFLLMTWYIAWLLFRIFSTEAHHFPVQPTFAEEADQCLPKILNSNPPPLLKFLALQDLMFLSQYSSVRRQEVFSLSQPGGHPYNWTSICGECLNLLSDLTQKLIIQQEIAAANGRLKQPSAGDLPQSFSSPAPEESSFQTPRTSIFPRAHMYSLVKSSLLPLKTSPASDLGSNLGSPFNTSLLGHKPGLLELNSPWHGSVQSPHIMRRGPKLWTSSSDLQLTGYPDEPHPVMVVAKDAKEAAEPGCFYMWLQNKKEQIRNFLSKRVLIMYFFNKHPEASIQALFSDTQIHIWALEGLSHLVATSFTEDRYGVVQTTLPAILTTLLTLQEVVDKHFKLPHVSSKPPKTSGNLADTSYKTLRFALRSSLKTAIYRITTTFGEHLNAVQVSSEHKKRLQQFLDFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJR7</accession>
    <name>A0A6I9XJR7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cyclin-L1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CCNL1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclin family. Cyclin L subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911138.1">
        <property type="nucleotide sequence ID" value="XM_014055663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJR7"/>
    <dbReference type="GeneID" id="106540523"/>
    <dbReference type="KEGG" id="tsr:106540523"/>
    <dbReference type="CTD" id="57018"/>
    <dbReference type="OrthoDB" id="10264655at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20592">
        <property type="entry name" value="CYCLIN_CCNL1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000014">
        <property type="entry name" value="cyclin-L1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000016">
        <property type="entry name" value="cyclin-L1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036580">
        <property type="entry name" value="Cyclin_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="85"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="12">
        <location>
            <begin position="196"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="200"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="315"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="379"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="423"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="506"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911138.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="59778" checksum="DBDF93498B0C1350" modified="2020-10-07" version="1">MASAGIHVSPAVVCAPLSSAPPPPPPPPPPPALPAGILIGDRLYSEVSLTIDHSLIPEERLSPTPSMQDGLDLQSETDLRILGCELIQAAGILLRLPQVAMATGQVLFHRFFYSKSFVKHSFEIVAMACINLASKIEEAPRRIRDVINVFHHLRQLRIKRTPSPLILDQNYINTKNQVIKAERRVLKELGFCVHVKHPHKIIVMYLQVLECERNQTLVQTAWNYMNDTLRTNIFVRFQPEAIACACIYLAARALQIPLPNRPHWFLLFGTTEEDIQEICLKILKLYTRKKPNYELLDKEVEKRKMTLQEAKLKAKGLNPDGTPALSTLGGFSPASKPSSPREIKTEEKSPVCINAKTIKKEPEDRQMVSKSPYNGIRKESKRSRSSRSGSRSKSRTKSRSRSHSPRRHYNNRRSLSGTYSSRSRSRSRSHSGSPRRHHNHGSPHLKPKHSREELKGVNRHSHKRKKSRSRSQSKPRDHSDLIKKHRHERGHHRDRRERSRSFERSHKSKHHGAGRSGHSRHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJW5</accession>
    <name>A0A6I9XJW5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mannosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PIGB</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Alpha-1,2-mannosyltransferase that catalyzes the transfer of the third mannose, via an alpha-1,2 bond, from a dolichol-phosphate-mannose (Dol-P-Man) to an alpha-D-Man-(1-&gt;6)-2-PEtn-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol intermediate to generate an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)-2-PEtn-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H6) and participates in the nineth step of the glycosylphosphatidylinositol-anchor biosynthesis. May also add the third mannose to an alpha-D-Man-(1-&gt;6)-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H3) intermediate generating an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H4).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_013914227.1">
        <property type="nucleotide sequence ID" value="XM_014058752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJW5"/>
    <dbReference type="GeneID" id="106542894"/>
    <dbReference type="KEGG" id="tsr:106542894"/>
    <dbReference type="CTD" id="9488"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 9 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013914227.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="40384" checksum="6340748C1F538796" modified="2020-10-07" version="1">MEAVLTIFALCYYPMESTKTEISRCYLALVALACIIRPTAAIPWAPLLLWHFGKEPNKRRFLWGACLPVGLIALGGSLVVDRIFFGKWVVVPLNFLKFNVLHELSTFYGSHPWHWYFTQGLPAILGPHLPFFLHGCFSAPRKHRVFLVVVLWTVAAYSVLRHKEFRFIYSILPVCMLFCGHSLAQLKRWRRAAVSFLLSSNLLLALYTGLVHQRGTLDVMVYLRELCNPLARGQASSILLLMPCHSTPLYSHIHCPLQVRFLQCPPDLMGRSNYLDEADLFYSRPLLWLHKEFPNATQLPSHLVFFNVLEQEISSFLISNKYVKAATFFHTHLPQGRVGSHIYVFKNYFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKV1</accession>
    <name>A0A6I9XKV1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106544640</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Regulates neuronal differentiation in response to NGF by facilitating NGF-mediated activation of NTRK1/TRKA receptor tyrosine kinase and subsequent downstream signaling pathways. Plays a role in the inhibition of TNFalpha-induced apoptosis. Mechanistically, inhibits the NF-kappa-B signaling pathway by blocking phosphorylation of CHUK. Also promotes the stability of the thiamine transporter 1/SLC19A2 in intestinal epithelial cells leading to an increase of thiamine uptake process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SLC19A2. Interacts with NTRK1/TRKA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916449.1">
        <property type="nucleotide sequence ID" value="XM_014060974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKV1"/>
    <dbReference type="GeneID" id="106544640"/>
    <dbReference type="KEGG" id="tsr:106544640"/>
    <dbReference type="OrthoDB" id="6134317at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03156">
        <property type="entry name" value="uroplakin_I_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF216">
        <property type="entry name" value="TETRASPANIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013916449.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="26546" checksum="40F67CEDF31403E1" modified="2020-10-07" version="1">MSCFTFLKVMMILFNLLIFLGGATLLGIGIWVTVDSKSFLNIFGAISVTVLQFVHVGYLLIAIGAVLFLLGFLGCCGAQKESKCLLIMFFTIILIIFIIEIAGAVVALVYTSLAQTILQSTVAKILKDDYGKPNPLTEAWNVTMTNLKCCGLSNYTDFNNSYFYKESKNFYPGQCCNSSISSLSDTFCNTAMAERFNVTGCFEQMLSEIHKNAAVVGGVAAGIGALEIGAMAVAMYLYCKMDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNI2</accession>
    <name>A0A6I9XNI2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Thrombospondin type-1 domain-containing protein 7A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">THSD7A</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915451.1">
        <property type="nucleotide sequence ID" value="XM_014059976.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543873"/>
    <dbReference type="KEGG" id="tsr:106543873"/>
    <dbReference type="CTD" id="221981"/>
    <dbReference type="OrthoDB" id="5814848at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000014">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000017">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000018">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000019">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000020">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000027">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000031">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000050">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000015">
        <property type="entry name" value="Thrombospondin, type I, domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051418">
        <property type="entry name" value="Spondin/Thrombospondin_T1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044004">
        <property type="entry name" value="TSP1_spondin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056991">
        <property type="entry name" value="TSP1_TSH7A-B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11311">
        <property type="entry name" value="SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11311:SF8">
        <property type="entry name" value="THROMBOSPONDIN TYPE-1 DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19028">
        <property type="entry name" value="TSP1_spondin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23308">
        <property type="entry name" value="TSP1_TSH7A-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1614"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="184"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="350"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="644"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="1045"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="1295"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="1423"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="domain" description="Thrombospondin type-1" evidence="18">
        <location>
            <begin position="1527"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF19028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013915451.1"/>
        </source>
    </evidence>
    <sequence length="1664" mass="187168" checksum="0A42BDDD5A866A66" modified="2020-10-07" version="1">MEMAEQYIAFLGGVVVSAHSSKCLEVLALLWFAIWVATAQSELETSVYLWKTGPWGRCMGDECGPGGTQTRAIWCAHVEGWTTLFTNCKQAERPDNQQNCFRVCDWHKELYDWQLGSWNQCQPILSKSIEKTTVCVNGEEGIQRRDIICVHKSNGVIVEDAICEYFEPKPQLEQGCLIPCPQDCIVSDFSAWTECSKSCGKGLSYRFRHIIAPPRYGGSDCPNLTEFQVCSSALCATEENLYSLSVGSWSTCSIPHLRHVRQARKRGKNKEKEKEKEKNIRDPETRELIKKKRTHNRQNRQENKYWDIQIGYQTRQVMCTHRNGKTVALSFCQQEKLPITFQSCVITKECQVSEWSEWSPCSKTCFDMTSSKGYRTRTRTIKQFPIGSESECPNLEETEQCVTQGDGVTACITYGWRTTEWTECRIDPLLSQQDKRRGNQTSLCGGGIQTREMYCVQANENLLSYLNTLKEKKESQSQRTSKTSAAPLLSITFEITASKPVDWKLCTGNTPSTTQLCHIPCPIECEVSAWSAWGLCTFENCEDQQAKKGFKLRKRRIKNEPTGGTGNCPHLVEAIPCEEPSCYEWKIVSLDECIPDNEKNCGPGTQVPQVVCISSDGEEVDRQLCRDAIYPVPIVCEIPCPKDCVLSTWSGWSSCSHTCSGKTTEGKQMRTRSILAYAGEGGLQCPNSSMLQETRNCNDHPCTVYHWQTGQWGQCIEDTSVSAFNSSAGWNGEATCAVGMQTRKVICVRLNMGQVPPKKCPENLRPETVRPCLLPCRKDCIVTPYSDWTLCPSLCQEGSVVTVTKQSRHRVILQLPANGGRDCPETLYEEKDCDPPPVCVFYRWKTHKWRRCQLVPWYVRQDSPGAQETCGPGLQARAITCRKQDGSQADIGECLKYAGPLPPLTQLCQIPCQDDCLFTIWSKFSACGLDCGSVRTRKRLLIGKSKKKEKCKNPQLYPLIETQFCQCDKYNAQPVGNWSDCILPEGKVDILMGMKVQGDIKECGQGYRYQAMACYDQSSRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCGQYIGVTEPWSVCKVTNVDLKENCGVGVQTRKVRCMQNTVDGPSDIVEDYLCDQEEMPMGARKCILPCPEDCVISEWGQWSKCSLPCNGSSVRERLAKILRLPQEGKACPSTVETEPCHLNKNCYHYDYNVTDWSTCQLSEKAVCGIGIKTRMLDCVRSDGKSVDLLYCDKLGLEKTWNMNTSCMVECPVNCQLSDWSPWSECSQACGLTGRMTRKRTVIQPFQGDGRPCPSQMEQFKPCPVKPCYRWQYSSWEECKVEDAQCGEGTRTRNIFCVVYDGSSDDTGKKVDEEYCGEIEPMVDGNKKIVLEEACTVPCPGDCYLKEWSHWSICQLTCINGEDLGFGGIQVRSRAVIVQDVENQHLCPNQALGTRQCNDGHCYEYNWMASAWKGSSRTVWCQRSDGLNVTGGCLMMHQPDVDRSCKPACSQSHSYCSETRMCVCEEGYTEVMSSNGTLEQCTLIPVVVIPTVDHKEDVKTSRAVNPTQTPITTPGRGRTWFLQPFAPDGKLKTWVYGVAAGAFVLLIFVISMVYLACKKPKKPQRRQNNRLKPLTLAYDGDADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQN0</accession>
    <name>A0A6I9XQN0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Noggin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106544363</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the noggin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916086.1">
        <property type="nucleotide sequence ID" value="XM_014060611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQN0"/>
    <dbReference type="GeneID" id="106544363"/>
    <dbReference type="KEGG" id="tsr:106544363"/>
    <dbReference type="OrthoDB" id="5950649at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.520">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008717">
        <property type="entry name" value="Noggin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10494">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN INHIBITOR, NOGGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10494:SF6">
        <property type="entry name" value="NOGGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05806">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916086.1"/>
        </source>
    </evidence>
    <sequence length="270" mass="30915" checksum="13CC8C6AF33F55AF" modified="2020-10-07" version="1">MDVKVWLACMLLLEQWSPGFHYIEAIFASQKGGFSLSGAPRPSNNIRALEQSNPDIHLTWSKLPTQGKPYSLSLSPEDYHYSVPKPKHLRTSKLMKLLGSSYDAFWMSPEDPRGRNPSAEELSTQNRELAKHTARFKKTLLQETSRLQLPKLQLLLPFEDGIAGNLSQSFIYRLRRWLVDSATCSLTSSWVDMGSVFWPRWIRHTHCEQAGCSWPPGMTCQPAQITHIKLLAWHCWINGTQSLNRRKSRKECVWRQIPYPVVTACKCACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQQ0</accession>
    <name>A0A6I9XQQ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arrestin domain-containing protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106544593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916382.1">
        <property type="nucleotide sequence ID" value="XM_014060907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQQ0"/>
    <dbReference type="GeneID" id="106544593"/>
    <dbReference type="KEGG" id="tsr:106544593"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013916382.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="16905" checksum="EA916EC6A0EA226F" modified="2020-10-07" version="1">MSMSLENYQLASPQVNCLNLSSLSSQVYVHIPGAKKLMVELPLVIGTIPCTVFSSRNSDFVNQLAMDMRWLAQTLPDHPEAPPNYADIVSEEEYGRHVTVYPTSVDCEEQLPCPMLAYIQEFRFQPPPLYSEVDPYPPEVEDNLPVALMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQR9</accession>
    <name>A0A6I9XQR9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Laforin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EPM2A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918404.1">
        <property type="nucleotide sequence ID" value="XM_014062929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQR9"/>
    <dbReference type="GeneID" id="106546146"/>
    <dbReference type="KEGG" id="tsr:106546146"/>
    <dbReference type="CTD" id="7957"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019203">
        <property type="term" value="F:carbohydrate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044042">
        <property type="term" value="P:glucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14526">
        <property type="entry name" value="DSP_laforin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000054">
        <property type="entry name" value="laforin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045204">
        <property type="entry name" value="DSP_laforin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042942">
        <property type="entry name" value="Laforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46864">
        <property type="entry name" value="LAFORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46864:SF1">
        <property type="entry name" value="LAFORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="111"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="198"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013918404.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="32483" checksum="4ABF92F21E6B4E99" modified="2020-10-07" version="1">MRALVMKPSRPLAPLPAQEPVLWLGEVALPGEEEAAGTFWYKFLRRLETGDAVWEGNGPHHDRTNVYNPCNLVDGVYCLPIGHWIEVSGHTDEMKHTTDFYFNIAGHQAIHCSRILPNLWLGSCPRQVEHVTVKLKHELGITAIMNFQTEWDVVQNSWGCNRYPEPMSPEVLMKLYKEEGLAYVWMPTPDMSTEGRVQMLPQAVYLLHGLLENGHTVYVHCNAGVGRSTAAICGWLKYVLGWNLRKVQYFVASRRAAIYIDEEALVRAEEDFFQKFGPLRSSFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR58</accession>
    <name>A0A6I9XR58_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ELAV-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">ELAVL2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RRM elav family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916251.1">
        <property type="nucleotide sequence ID" value="XM_014060776.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013916259.1">
        <property type="nucleotide sequence ID" value="XM_014060784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR58"/>
    <dbReference type="GeneID" id="106544492"/>
    <dbReference type="KEGG" id="tsr:106544492"/>
    <dbReference type="CTD" id="1993"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12771">
        <property type="entry name" value="RRM1_HuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12775">
        <property type="entry name" value="RRM2_HuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12654">
        <property type="entry name" value="RRM3_HuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000006">
        <property type="entry name" value="ELAV-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000005">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000017">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006548">
        <property type="entry name" value="ELAD_HU_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034999">
        <property type="entry name" value="HuB_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034914">
        <property type="entry name" value="HuB_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01661">
        <property type="entry name" value="ELAV_HUD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="68"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="154"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="306"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013916259.1"/>
        </source>
    </evidence>
    <sequence length="389" mass="42592" checksum="C84E09DF9DB52888" modified="2020-10-07" version="1">MAVRLCDVASLLRSGSWAAEPWTGQVIAAMETQLSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKTNQAILSQLYQSPNRRYPGPLAQQAQRFRLDNLLNMAYGVKSRFSPMTIDGMTSLAGINIPGHAGTGWCIFVYNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSY8</accession>
    <name>A0A6I9XSY8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Kelch domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">KLHDC3</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907844.1">
        <property type="nucleotide sequence ID" value="XM_014052369.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907845.1">
        <property type="nucleotide sequence ID" value="XM_014052370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSY8"/>
    <dbReference type="GeneID" id="106538013"/>
    <dbReference type="KEGG" id="tsr:106538013"/>
    <dbReference type="CTD" id="116138"/>
    <dbReference type="OrthoDB" id="432528at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000016">
        <property type="entry name" value="Kelch domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000055">
        <property type="entry name" value="kelch domain-containing protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052637">
        <property type="entry name" value="KLHDC3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46461:SF3">
        <property type="entry name" value="KELCH DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46461">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013907844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907845.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="43322" checksum="65A738D072D79145" modified="2020-10-07" version="1">MLRWTVHLEGGPRRVNHAAVAVGHKVYSFGGYCSGEDYETLRQIDVHVFNAVSLRWTKLPPVWTNSRDHVREVPYMRYGHSAVLIDDTIYIWGGRNDTEGACNVLYAFDVSTHKWFTPKVSGMVPGARDGHSACVLGKSMYIFGGYEQLADCFSNDIHKLDSSNMIWSLICTKGTPARWRDFHSATIIGTKMYVFGGRADRFGPFHSNNEIYCNRIKVFDTDTNSWLDSPPTPLLPEGRRSHSAFGYNGELYIFGGYNARLNRHFHDLWKFDPVSFTWKKIEPKGKGPCPRRRQCCCRVGDKIILFGGTSPSPEEGMGDEFDLMDHSDLYILDFSPSLKTLCKLAVIQYNLDQSCLPHDIRWELSAMTTNSNISRPILSSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT53</accession>
    <name>A0A6I9XT53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metastasis-associated protein MTA2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MTA2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013917262.1">
        <property type="nucleotide sequence ID" value="XM_014061787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT53"/>
    <dbReference type="GeneID" id="106545279"/>
    <dbReference type="KEGG" id="tsr:106545279"/>
    <dbReference type="CTD" id="9219"/>
    <dbReference type="OrthoDB" id="2193595at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11661">
        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000012">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040138">
        <property type="entry name" value="MIER/MTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035170">
        <property type="entry name" value="MTA1_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865">
        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN AND MESODERM INDUCTION EARLY RESPONSE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865:SF4">
        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN MTA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17226">
        <property type="entry name" value="MTA_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="303"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917262.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="46752" checksum="321E62FD35A17022" modified="2020-10-07" version="1">MEEWSASEAMLFEEALEKYGKDFNDIRQDFLPWKSLASIVQFYYMWKTTDRYIQQKRLKAAEADSKLKQVYIPTYTKPNPNQIISVGSKPGMNGAGFQKGLTCESCHTTQSAQWYAWGPPNMQCRLCASCWIYWKKYGGLKTPTQLEGAARSITEPHSRGHTSRPEAQSLSPYTTSANRAKLLAKNRQTFLLQTTKLTRIARRMCRDILQPRRAARRPYAPINSNAIKAECSIRLPKAAKTPLKIHPLVRLPLATIIKELAAQAPLKPRTPRGTKTPINRNQLTPNRGLAGIVSKRTFENLCVRPGAGGRGRAPLLGQRTAPNVRHEVKQSACPETSEAEPGRRPQSCRFCGDQGHQSPAQGPEPPGDAPGCPPSQPAGEGEVIPATQASGDSPVARAPSPSCQHQRTHRPGRLSAELLPQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUZ6</accession>
    <name>A0A6I9XUZ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">INO80 complex subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">INO80D</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908470.1">
        <property type="nucleotide sequence ID" value="XM_014052995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUZ6"/>
    <dbReference type="GeneID" id="106538500"/>
    <dbReference type="KEGG" id="tsr:106538500"/>
    <dbReference type="CTD" id="54891"/>
    <dbReference type="OrthoDB" id="10038011at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025927">
        <property type="entry name" value="Znf_KANL2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16198">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16198:SF2">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13891">
        <property type="entry name" value="zf-C3HC3H_KANSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KANL2-like probable zinc-finger" evidence="4">
        <location>
            <begin position="18"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="677"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="853"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="874"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013908470.1"/>
        </source>
    </evidence>
    <sequence length="892" mass="98126" checksum="64AD039D74CDF6D5" modified="2020-10-07" version="1">MYEGKHIHFSEVDNKPLCSYSPKLCKQRRLNGYAFCIRHVLEDKTAPFKQCEYVAKYNSQRCTNPIPKSEDRRYCNSHLQVLGFLPKKERKKKNDPTEEVKVRHQMDSIAFSLTVPPLALKMSNGLVGMSLSPPGARLPLHYLEAELEDPFAFNEEDEELKKGTAVKKKLQSKLAQNRQRQKETEIQKVRQEHFSPAPAPSQQPPLQPQQHAHLSPLPTSLKPAVHPQGLVCKSPQPQNTSLPMQGGAPTTHAIAQARQSANKRPLPLLHPTKVHAPDPPRADRILMKATAFSPYSFCRSRLQRLVKLCTQKHQLDADLFPHLGLDWSEDSGEELEELDQTSPYQVAWSIREALRYERNEPDDDYASGRRTRISQLCTYFQQKYKHLCRLERAETQQRKCQHTFRKALLRAANREPEYAGQLIRELHRASRPRTSSSQVKQRDASPCGGTTKGEPCTNKALPFTKHCFQRILFIVPLFICACLKVYVRERLILYFLAFCFDEGKNGDLLPTTEEAEELERALQAVTSLECLSTIGVLTQTDGVPVQELSDRGIGVFSPGAESSGIQSLSREVNADLGELLNGRIVHDDNFSGLDLDENLLHSATLSNPSTPLAGQIQGQFSAPANVGLTSATLISQSALGERAFPGQFQGLHDGSHASQRPHPAQLLSKADDLITSRQQYSSDHSHSSPHGSHYDSEHVPSPYSDHLRSPSSTSYSSDNLAATFSTEMPIIAQHLLPSQLEVPLSGVVNPRTHWGSLPVNLGDPSPFSNLLGADGHLLSTSLSTPPTTSHSETTQPAFATVTPNSSSVLPGLPQTSFSGMGAASSELMASTSPKQQLPQFSAAFGHQLSSHSGIPKDLQPSHSSIAPPTGFAVTGATATSTNNASAPFTTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVZ7</accession>
    <name>A0A6I9XVZ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Stearoyl-CoA desaturase 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106547652</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The histidine box domains are involved in binding the catalytic metal ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920354.1">
        <property type="nucleotide sequence ID" value="XM_014064879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVZ7"/>
    <dbReference type="GeneID" id="106547652"/>
    <dbReference type="KEGG" id="tsr:106547652"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001522">
        <property type="entry name" value="FADS-1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF100">
        <property type="entry name" value="STEAROYL-COA DESATURASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00476">
        <property type="entry name" value="FATTY_ACID_DESATUR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="4 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="17">
        <location>
            <begin position="75"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013920354.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="38027" checksum="70F7F97216992B7D" modified="2020-10-07" version="1">MSKLGAKNVSDGETDMSCDGQAESPFGSREDEIKEKVDHRQPIVWKNVILMALLHSGSVYSLLLIPKAHLYTLIWAYICFLVCALGVTAGAHRLWSHRSYKARLPLRIFLAAANSMAFQNDIFEWARDHRVHHKYSETDADPHNAQRGFFFSHIGWLFVRKHRDVIEKGRKLDFNDLLEDPVVTFQRKYYRSSVVLMCFVIPTIVPWHFWGESIWNSYFLASILRYTVSLNVTWLVNSVAHMYGNRPYDKSINPRQNHFVTFGAIGEGFHNYHHTFPFDYSASELGLKFNPTTWFIDLMFWLGLVTERKQAPSEMIKARKERTGDGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWM8</accession>
    <name>A0A6I9XWM8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Testis-specific serine kinase substrate</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TSKS</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920584.1">
        <property type="nucleotide sequence ID" value="XM_014065109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWM8"/>
    <dbReference type="GeneID" id="106547840"/>
    <dbReference type="KEGG" id="tsr:106547840"/>
    <dbReference type="CTD" id="60385"/>
    <dbReference type="OrthoDB" id="9424665at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028214">
        <property type="entry name" value="TSKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14351">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14351:SF1">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15358">
        <property type="entry name" value="TSKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920584.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25550" checksum="0FACE8C450B36F66" modified="2020-10-07" version="1">MLQEVSNSGLLRAKDSITSLKEKTSKVTHHLQNLQESCRKVTKSVEDAEIKTSVLEENSAQLEEKLRNLQQQVQVEDFRWKCMLELAQKEVLRLLAQLGLCLEGVLPQRDRVQVEQGQELSTLREELDEVSAAAKRASVSLTQIRIDLDGLLKDISRHLATLKNMEASDRPPPNCARCSSYSIVNAEVLRKMFEHAMAPILEELKQRGQSEGKCPGCQRLQKTMSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWW7</accession>
    <name>A0A6I9XWW7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ARV1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915118.1">
        <property type="nucleotide sequence ID" value="XM_014059643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWW7"/>
    <dbReference type="GeneID" id="106543590"/>
    <dbReference type="KEGG" id="tsr:106543590"/>
    <dbReference type="CTD" id="64801"/>
    <dbReference type="OrthoDB" id="2192830at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013915118.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="27264" checksum="98720F7C2454F832" modified="2020-10-07" version="1">MEEFACYRCIECSTKATELYKDYQRGVLRISICKSCQKPVDKYIEYDPVIILINAILCKAQVYRHILFNTKINIHGKLCIFCLLCEAYLRWLQLQDSSQSTEPDDLIRYAKEWDFYRMFGITSLEQTAFLTGIFTALWLIKPAVLKTKADFIFLLKALLLSSYGKLLLIPAVIWEHDYTHLCLKLIKVFVLTSNFQAIRVTLNTSRKLCLMVILNGLLFENGIIYLFQKIGWDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXF1</accession>
    <name>A0A6I9XXF1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Toll-like receptor 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">TLR4</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918852.1">
        <property type="nucleotide sequence ID" value="XM_014063377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXF1"/>
    <dbReference type="GeneID" id="106546496"/>
    <dbReference type="KEGG" id="tsr:106546496"/>
    <dbReference type="CTD" id="7099"/>
    <dbReference type="OrthoDB" id="1421090at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046696">
        <property type="term" value="C:lipopolysaccharide receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001875">
        <property type="term" value="F:lipopolysaccharide immune receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032497">
        <property type="term" value="P:detection of lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002755">
        <property type="term" value="P:MyD88-dependent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000006">
        <property type="entry name" value="Toll-like receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017241">
        <property type="entry name" value="Toll-like_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF521">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037595">
        <property type="entry name" value="Toll-like_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="670"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="22">
        <location>
            <begin position="724"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013918852.1"/>
        </source>
    </evidence>
    <sequence length="885" mass="100311" checksum="9CC060921FB9B3AC" modified="2020-10-07" version="1">MKPRLPNPRILEFLGYLIDGSGIHPTNKKIKAIQEAPTPRNQTELQAFLGLFNFYIVFLDLVAEPLHWLLGKAATWKWEKFKDATFAAVKKLISVSIGWLIVLTPDSVALASAGVCKDTDNTPPQNFLLVSLWCHLWKIEDRAFMGLNSLSVLILTGNPLQILGPRAFHSLPSLQRFVAVETNLFSLAMLPIGHLTSLEELNLSHNHIGSLKLPGYFSNLVSLRHLSFQLNEISSISVGDLDPLASRDVMLVLSRNDLKHIERGALAGIRLRQLVLRGCFEDRAVMQSSLQNMSGLHIDSLLLGEYRDIDKVDFMDRTLLDVLCDLHLQELTVIEMYALGSGSTCSPCLNNISMVRVVHTNVREPFEFPSNSNISQLEFKNCALDAVPATILSALSKLRVLRITHTKYLRDFETYFKGLQHLETLDLSVNRLVTHLSWVGLLEGAPNLKHLNLSFNTEIRLNIECSGPGKLEYLDLQHTALATPGTVPSFFCLHRLIYLDISYTDTAVIAQGSFHGLHSLQVLKMAGNSFANNQLVDNFRNLTKLHFLDISNCQLSHISLSSLTSLRELRLLNVSHNKLLGLHPETYLHIPLLTTLDFHNNQLSALTEEDLKNLPAGLKYLDLSENLFDCSCNHAGFLRWAKNSSALLQNVQHMLCYSPPELKNVRVMDFVLASCGISITTVAVSVTVVLVLIVIAILCYKYYFYLYYGMVLFLRNRSSDEKETIYDAFVIFSSKDQEWVKQELQQPLEEELPYFRLCLFYRDFIPGVSIITNIIKEGFQSSRKVIAVVSDDFLESRWCNFELEVAQSWQLVESKASIIFVVLEGVDKAALNRKLGLFRYLRRNTFLNWKDRDLSRHVFLRQLRSALLEGKTWTEDELKLMLKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYA0</accession>
    <name>A0A6I9XYA0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PIP5K1A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921214.1">
        <property type="nucleotide sequence ID" value="XM_014065739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYA0"/>
    <dbReference type="GeneID" id="106548377"/>
    <dbReference type="KEGG" id="tsr:106548377"/>
    <dbReference type="CTD" id="8394"/>
    <dbReference type="OrthoDB" id="70770at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF54">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE TYPE-1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013921214.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="41807" checksum="105CFF3D3B46D139" modified="2020-10-07" version="1">MNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVRMHHKYDLKGSTYKRRASQKEREKVFPTYKDLDFLQDLPDGLFLDLDMHSALCKTLQRDCLVLQSFKIMDYSLLVAVHNLDLAQRERAVADGASLADTRRPAGQRALYSTAMEAIQGEARRGGPIETDDQMGGIPARNAKGERLLLYVGIIDVLQSYRFMKKLEHSWKALVHDGDTVSVHRPSFYAERFQRFMCQAVFKKIPLKSSPSKKSRSGMGPPRRPGQTGTPAQALSETKVQVTMEAEMEPGSQLGRPDLLPQTPPVEEANSDSAATTLSTSSLGSPGHSSPASRSVGAEAPPPDTPKDLAVFHLESASPSPSVTGHPSELIQPLGDLAETEINC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYI6</accession>
    <name>A0A6I9XYI6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Partner of Y14 and mago</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">WIBG</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts (via N-terminus) with magoh and rbm8a; the interaction is direct. Associates (eIF2A-like region) with the 40S ribosomal subunit and the 48S preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pym family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915663.1">
        <property type="nucleotide sequence ID" value="XM_014060188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYI6"/>
    <dbReference type="GeneID" id="106544046"/>
    <dbReference type="KEGG" id="tsr:106544046"/>
    <dbReference type="CTD" id="84305"/>
    <dbReference type="OrthoDB" id="21625at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903259">
        <property type="term" value="P:exon-exon junction complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039333">
        <property type="entry name" value="PYM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015362">
        <property type="entry name" value="WIBG_mago-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036348">
        <property type="entry name" value="WIBG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959:SF0">
        <property type="entry name" value="PARTNER OF Y14 AND MAGO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959">
        <property type="entry name" value="PYM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09282">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01273">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101931">
        <property type="entry name" value="Pym (Within the bgcn gene intron protein, WIBG), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIBG Mago-binding" evidence="4">
        <location>
            <begin position="10"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="53"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013915663.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="23861" checksum="79E61791DB16520A" modified="2020-10-07" version="1">MATPYVTDETGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYVKFFKSKPELPPGLNPEVQTPVARQTSKGPEGGETGLSKTAKRNLKRKEKRKQQHEKGDRDTDDLIQSLEKTTLSATPSSCGDGKLAVSDANPRTPAGSRDVSSTSATLEKNKKVKNLKKKLRQVEDLQARIDSGEIKQPTKEQLEKLARRKALEEELEDLELDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYS8</accession>
    <name>A0A6I9XYS8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neuroblastoma suppressor of tumorigenicity 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NBL1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919001.1">
        <property type="nucleotide sequence ID" value="XM_014063526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYS8"/>
    <dbReference type="GeneID" id="106546616"/>
    <dbReference type="KEGG" id="tsr:106546616"/>
    <dbReference type="CTD" id="4681"/>
    <dbReference type="OrthoDB" id="8196271at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038098">
        <property type="term" value="P:sequestering of BMP from receptor via BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004133">
        <property type="entry name" value="DAN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15283">
        <property type="entry name" value="GREMLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15283:SF5">
        <property type="entry name" value="NEUROBLASTOMA SUPPRESSOR OF TUMORIGENICITY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03045">
        <property type="entry name" value="DAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027005239" evidence="6">
        <location>
            <begin position="17"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="7">
        <location>
            <begin position="36"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013919001.1"/>
        </source>
    </evidence>
    <sequence length="163" mass="17964" checksum="A6923D1DE0440557" modified="2020-10-07" version="1">MLRLLLSAFLPCLSLSAPSHINKLALFPDKNAWCEAKNITQIIGHHGCNSKSIQNRACMGQCFSYSVPNTLPQSTESLVHCDSCMPSNIKWDVVSLDCPQNGYMSKVEKLVEKILHCSCQACAMVHPSSNIEELPHQEASNPSAFRHLFEGEAAKPQPPSQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ88</accession>
    <name>A0A6I9XZ88_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription elongation factor SPT5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106546774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919206.1">
        <property type="nucleotide sequence ID" value="XM_014063731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ88"/>
    <dbReference type="GeneID" id="106546774"/>
    <dbReference type="KEGG" id="tsr:106546774"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919206.1"/>
        </source>
    </evidence>
    <sequence length="92" mass="10448" checksum="03AA82DAD662AE0A" modified="2020-10-07" version="1">MSDSEDSNFSEDESERSSEPEEVEENEAEERASVVGSEKEEAEEEEEEEEEEYDEEEDEDDDRPAKKPRHGGFILDEAGKNALPFMVGVFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZM0</accession>
    <name>A0A6I9XZM0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 56-B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106546913</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919376.1">
        <property type="nucleotide sequence ID" value="XM_014063901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZM0"/>
    <dbReference type="GeneID" id="106546913"/>
    <dbReference type="KEGG" id="tsr:106546913"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF1">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="44"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013919376.1"/>
        </source>
    </evidence>
    <sequence length="177" mass="20125" checksum="E5E1F6DB554458ED" modified="2020-10-07" version="1">MTIFNKLNLGIAVSSFAIFQLSFHVLSSWLSSRLTSGFNNLDQKKKIEWNTRTVSTVHALVVGIFCMYILLCDEAVNADRVWGDPSTVQLNLDISVGYLISDLLLIIFHWKAIGDIYFVIHHVLAVYAYYFVLVSSMLILYHNKYTCVLAKMDFNRLTIKINGSSQEDSNCSMVLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3M9</accession>
    <name>A0A6I9Y3M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear receptor-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">NRBP1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Homodimer. Binds to MLF1, recruiting a serine kinase which phosphorylates both itself and MLF1. Phosphorylated MLF1 binds to YWHAZ and is retained in the cytoplasm. Interacts with ELOC/TCEB1, ELOB/TCEB2, TSC22D2 and TSC22D4. Interacts with the Elongin BC E3 ubiquitin ligase complex via its interaction with ELOB/TCEB2 and ELOC/TCEB1. Interacts with SALL4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918063.1">
        <property type="nucleotide sequence ID" value="XM_014062588.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013918064.1">
        <property type="nucleotide sequence ID" value="XM_014062589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3M9"/>
    <dbReference type="GeneID" id="106545887"/>
    <dbReference type="KEGG" id="tsr:106545887"/>
    <dbReference type="CTD" id="29959"/>
    <dbReference type="OrthoDB" id="1034557at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14034">
        <property type="entry name" value="PK_NRBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000181">
        <property type="entry name" value="Nuclear receptor-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000098">
        <property type="entry name" value="Nuclear receptor-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="71"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013918063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013918064.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="64231" checksum="CA0E0F877010D062" modified="2020-10-07" version="1">MSEGETKQVSGNIGSDHKPEAASAGSGMTSLSPPLATATQEEEEEEEEEDESEDESEILEESPCGRWQKRREEVNQRNVPGIDSAYLAMDTEEGVEVVWNEVQFSERKNFKLQEEKVKAVFDNLIQLEHLNIVKFHKYWADVKENKARVIFITEYMSSGSLKQFLKKTKKNHKTMNEKAWKRWCTQILSALSYLHSCDPPIIHGNLTCDTIFIQHNGLIKIGSVFHRIFANVAPDTINNHVKTCREEQKNLHFFAPEYGEVAHVTTAVDIYSFGMCALEMAVLEIQGNGESSYVPQEAINNAIQLLEDPLQREFIQKCLEPQPSKRPTARELLFHQALFEVPSLKLLAAHCIVGHQHMIPENALEEMTKNLDMSAVLAEINHADRDGVRMIFSQSPALELDKFLEDVRNGIYPLTAFGLPRPQQPQQEVVKSPIAPPSVKTPTPEPAEVETRKVSSWSLIRSSLADNSKASPYHTPLSFQVLLMQCNIESVEEGVKHHLTLLLKLEDKLNRHLSCDLLPSDNIQELAAELVQLGFISEADQSRLSCLLEEAFNKFFYSRNGALAAVTVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4U8</accession>
    <name>A0A6I9Y4U8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Transcription factor Sp5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SP5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919248.1">
        <property type="nucleotide sequence ID" value="XM_014063773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4U8"/>
    <dbReference type="GeneID" id="106546803"/>
    <dbReference type="KEGG" id="tsr:106546803"/>
    <dbReference type="CTD" id="389058"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22541">
        <property type="entry name" value="SP5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000061">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF29">
        <property type="entry name" value="TRANSCRIPTION FACTOR SP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013919248.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="40246" checksum="5869C387B07F9304" modified="2020-10-07" version="1">MAAVAVLRNDSLQTFLQDRTPSASPDLAKHSPLALLAATCSRIGQPSAPVAPSDFLQVSYEASLGSPSRIFHPWSSDMPAHSPGGLPPPPPSLGLTPQPAFAAGHELPLTPPADPSYPYEFSPVKMLPASMGALSSACPPPAYVPYAAQAALPPGYSNLMPPPPAPQSCRQLSPGPEDLPWWSIQQAAPGPGTCAHRFAGGLQRGLVLAPSELAQYQSQLAALLHPKAPLAATARRCRRCRCPNCQVAAGNGPDATAEPGKKKQHVCHVPGCGKVYGKTSHLKAHLRWHTGDRPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFGCAECGKRFMRSDHLAKHVKTHQNKRLKAGLGLPGVDGRACAIKREESRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y635</accession>
    <name>A0A6I9Y635_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Staphylococcal nuclease domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SND1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913670.1">
        <property type="nucleotide sequence ID" value="XM_014058195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y635"/>
    <dbReference type="GeneID" id="106542461"/>
    <dbReference type="KEGG" id="tsr:106542461"/>
    <dbReference type="CTD" id="27044"/>
    <dbReference type="OrthoDB" id="10023235at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00175">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000002">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000004">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="1">
        <location>
            <begin position="21"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="1">
        <location>
            <begin position="196"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913670.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="31076" checksum="BC602CBB5ABA4296" modified="2020-10-07" version="1">MASSAGPAAASTAAGSAQAPALQRGIVKMVLSGCAIIVRGQPRGGPPPERQINLSNIRAGTLARRAAAGQPDGKDTPDEPWAFPAREFLRKKLIGKDVCFSVEYKTSPRREYGMVYLGKDTSGENIAESLVAEGLACRREGVRANNPEQSRLAELEEQAKTAKKGMWSEGTGSHTLRDLKYTIENPRHFVDSMHQKPVNAIIEHVRDGSVVRALLLPEYYLVTVMLSGIKCPTFKREADGTETPEPFAAEAKFFTESRLLQRDVQIVLESCHNQNILGTILHPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y700</accession>
    <name>A0A6I9Y700_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein kinase C</fullName>
            <ecNumber evidence="5">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC106543054</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013914430.1">
        <property type="nucleotide sequence ID" value="XM_014058955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y700"/>
    <dbReference type="GeneID" id="106543054"/>
    <dbReference type="KEGG" id="tsr:106543054"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20843">
        <property type="entry name" value="C1_PKD2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000056">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000137">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000019">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF12">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
        <location>
            <begin position="146"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
        <location>
            <begin position="265"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="22">
        <location>
            <begin position="398"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="552"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="326"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013914430.1"/>
        </source>
    </evidence>
    <sequence length="663" mass="73509" checksum="AC216468CEAB3C6D" modified="2020-10-07" version="1">MGWSLGQRERETAGKASEEPAGVWMASLPAAPAPVGAPEPIQPAASPASPAGVSFQLQIGLTREYVLLPAACDLAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDSSTANILQLVRAASDIQEGELVEVVLSASATFEDFQIRPHALNVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSSARKRRLSSTSLVNSQSLRLSTTESLPCTPDELSRSSTELFPRRLTSSTYIGRPIELDKLFLSKVKVPHTFLIHSYTRPTVCQFCKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEASFNGGDMPMEESSDLSEADKNSLMDESDDSGIIPGSHSENDLRMDEDVESLQKSQSSMGYIPLMRVVQSVRQTTRKSSTALKEGWMVHFSNKDPLRKRHYWRLDSKCLTLFQNNSSNRYYKEIPLSEILTVEPAQDFSLVPSGANPHCFEIITANATYYVGENGAPSAPQHGGDGLEHAKAWETALRQALMPVVLQGVPAAQGQPPHRQASLRISVSNSQVQENVDISTVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQVEMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8D7</accession>
    <name>A0A6I9Y8D7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-tocopherol transfer protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TTPAL</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916745.1">
        <property type="nucleotide sequence ID" value="XM_014061270.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106544870"/>
    <dbReference type="KEGG" id="tsr:106544870"/>
    <dbReference type="CTD" id="79183"/>
    <dbReference type="OrthoDB" id="6682367at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF130">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916745.1"/>
        </source>
    </evidence>
    <sequence length="177" mass="20112" checksum="164342AADDCC0BF9" modified="2020-10-07" version="1">MYLTLEKLIQSEETQVNGIVILADYKGLGLSKASHFGPFIAKKMVGILQDGFPIRIKAVNVINEPRIFKGIFAILKPFLKEKIVKRFFLHGSDLQSLHHNLPPRILPEEYGGTAGKLDISSWNELLLASEDDFKYDDSHLPFSSDCSPHDPLMSEEIDENQHDDSLRSFKTQLYSCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9N6</accession>
    <name>A0A6I9Y9N6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">JmjC domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">KDM8</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918990.1">
        <property type="nucleotide sequence ID" value="XM_014063515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9N6"/>
    <dbReference type="GeneID" id="106546607"/>
    <dbReference type="KEGG" id="tsr:106546607"/>
    <dbReference type="CTD" id="79831"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000019">
        <property type="entry name" value="Bifunctional peptidase and arginyl-hydroxylase JMJD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056520">
        <property type="entry name" value="ARM_KDM8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF106">
        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND ARGINYL-HYDROXYLASE JMJD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24472">
        <property type="entry name" value="ARM_KDM8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="15">
        <location>
            <begin position="263"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013918990.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="45156" checksum="7A67FA385242FD18" modified="2020-10-07" version="1">MSGDGAAWAALEPAEVLRAARAGPGVGAAEVGAGAAALLSETAALLGQAEAADGWPAAARDGCLRAAERLGDWAWERLQEGPWHCVGRAWRRLFAYACLLRALCLCRARGPCAEALRACDMGLLLGAPGPELPRLAALLQRLLPAPEAPPRKPRTERPPAQQQEEEEGAAAAAEGTPVTRARRPSLQRFRERFLLPGAPLVLEGVVEHWPCLRKWSVDYLLQLAGNRTVPVELGARYTDQDWSQNLMTVAEFIRQFVEQNPKGYLAQHQLFDQIPELKEDIGIPDYCCLGKNEEEEITINAWFGPSGTISPLHQDPQQNFLVQVMGRKYVRLFSPDQSEKLYPHEGHLLHNTSQVDVEHPDLVKFPNFKAAAFQDCILSPGEVLFIPASYWHYVRALDTSFSVSFWWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAU3</accession>
    <name>A0A6I9YAU3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aminopeptidase O</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921025.1">
        <property type="nucleotide sequence ID" value="XM_014065550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAU3"/>
    <dbReference type="GeneID" id="106548213"/>
    <dbReference type="KEGG" id="tsr:106548213"/>
    <dbReference type="CTD" id="84909"/>
    <dbReference type="OrthoDB" id="79562at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000008">
        <property type="entry name" value="aminopeptidase O isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033577">
        <property type="entry name" value="AOPep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46627">
        <property type="entry name" value="AMINOPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46627:SF1">
        <property type="entry name" value="AMINOPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="2">
        <location>
            <begin position="434"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921025.1"/>
        </source>
    </evidence>
    <sequence length="536" mass="59089" checksum="10915AAC7EA13D70" modified="2020-10-07" version="1">MELDITTDDLPLMANTNHMLVKHYVLDLDVDFRNHIVEGTIVIFLEPVRTHGKGESEQMEGASPSWSEEACRVSTSEACSVGVPDASARTTKSGCNDFAVCGKGENDTSDKNGSHGNREQASGISSSKNCCDIEIHGSEDFLLVLDCCDLCVLKVEEVDVAVVPGIETCTSSAKLMDAAKDPRNLRNQIVHELVTLPTNNWREQLQRYNHCSQAPACGELLFTTDTWSLEIRKAGIKLPKDFPRAIRLWYKTKPEGQSLSWTTDQSDRPCVYTVGSPINNRALFPCQEPPVAMSTWQATVRADACFVVLMSGENVAEPAKSEKGVLSWFYYVTMPMPASTFAIAVGCWAEVKRKSYETDVLKNDGLSSKGDVRLLEKACGHTPYPCRFQDPITSAQVIIPHRIFAPLCLKDSCKKKLLHLLPQCLSAAYDLLGTHPFSRLDVLIVPLNFSSLGMASPHIIFLSQSTLSGESNLCGTRLCHEIAHAWFGLAIGARDWTEEWLSEGFATHLEDAIWSAAQKVLPRYFSNLSTLPGVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAX7</accession>
    <name>A0A6I9YAX7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Lysophosphatidic acid receptor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LPAR5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921195.1">
        <property type="nucleotide sequence ID" value="XM_014065720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAX7"/>
    <dbReference type="GeneID" id="106548359"/>
    <dbReference type="KEGG" id="tsr:106548359"/>
    <dbReference type="CTD" id="57121"/>
    <dbReference type="OrthoDB" id="5781782at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048266">
        <property type="term" value="P:behavioral response to pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF6">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="31"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921195.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="42292" checksum="CC92533FCA9F952D" modified="2020-10-07" version="1">MSTTNVTECPDDIHQLQLIGYSLIFIAGLCLNVASLLIFLYYLKLKSVVAIYMCNLITSDLLFTISLPFRIYYYYMGKWPFGSFLCQVSGSLFQINMYGSCIFLMCINLDRFVAIVHPLRWRHLRRPKVARLLCCVVWVLILIGSVPVAIIHKGTSCIKKSGTNSNLKKNETVCFENFSKDLWQGKIFGLVILAEILGFLLPLTSVTACSIRIFQNLCQTHGSRSLRQEKTIRMLVVNLVIFIICFVPYNTILAAYGLIKAKVIITDSTTQASVRNALIIAMLLASMNCTLDPLIYYFNTEGFKNTLKRIRRGQAWDFEMGTLQTRTRKNKKAASYKAANIKQDSKQYPVQNSIAPFASPKIFLRSSIEDSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB55</accession>
    <name>A0A6I9YB55_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106548492</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921345.1">
        <property type="nucleotide sequence ID" value="XM_014065870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB55"/>
    <dbReference type="GeneID" id="106548492"/>
    <dbReference type="KEGG" id="tsr:106548492"/>
    <dbReference type="OrthoDB" id="9975554at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="58"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921345.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="37822" checksum="0D6CAD0D8C8C7EED" modified="2020-10-07" version="1">MYEPIYCGKQNKRTLRHFPCVNKSSLLQFIFQAFSTVPEIQHLIFTFFLLLYLIIFSANISILSIVCMVNSLHTPMYFFLASLSLLEICYITVIVPQMLASISDFHRTIPLVNCAIQMFFFTTLGSTDCFMLAVMAYDRYVAICHPLRYTLIMTWQVCMWLVIGSLILAIVLCLQLTVLVFNLPFCGYQPKINHFFCDVPPVLQVACADTHLHQTALYGVVFIVLMIPFVLICISYVYIVAAILRIKSSSGRHKAFSTCSSHLTVVFLQYGVCSLVYLRPKSSTLEDEDKKLALIYTFVTPFLNPLIYTLRNKDIKQALRKVIKKMVASQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBR1</accession>
    <name>A0A6I9YBR1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">One cut domain family member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ONECUT2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CUT homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921557.1">
        <property type="nucleotide sequence ID" value="XM_014066082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBR1"/>
    <dbReference type="GeneID" id="106548660"/>
    <dbReference type="KEGG" id="tsr:106548660"/>
    <dbReference type="CTD" id="9480"/>
    <dbReference type="OrthoDB" id="10068888at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000005">
        <property type="entry name" value="One cut domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051649">
        <property type="entry name" value="CUT_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057:SF10">
        <property type="entry name" value="ONE CUT DOMAIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057">
        <property type="entry name" value="TRANSCRIPTION FACTOR ONECUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01109">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51042">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="9">
        <location>
            <begin position="308"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="263"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921557.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="47337" checksum="A895F86FFA239F60" modified="2020-10-07" version="1">MKAVCTPYRCLTKELESCAMNPELTMESLGPLHGPAGPEQDLMGSPSPHHHPPGRSAGPLRVHPPPAPPGPPPSHQELASASSRSAMVSSMASLLDGAGDYRPELSIPLHHAMSMPCDSSPPGLGMASTYTTLTPLQPLPPISTVSDKFHHPHAHHHHHHPHAHHPHAHHPHQHQRLSGNVSGSFTLMRDERGLPAMNNLYGPYKEMAGMGQSLSPLGNGLGSLHNPQQGLHNYGPAVPEKMLSPNFEAHPAMLTRSDQHLSRGLATPPAPMMAHLNGMHHPGGHPQPHGPVLASSRERPPSSSSGQVSNSGQLEEINTKEVAQRITAELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMWKWLQEPEFQRMSALRLAGLPFLFVSLIGVYRFKVTEEKGVGGSIKIHDWVVKNEALSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBR8</accession>
    <name>A0A6I9YBR8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NEDD4-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921545.1">
        <property type="nucleotide sequence ID" value="XM_014066070.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106548650"/>
    <dbReference type="KEGG" id="tsr:106548650"/>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14365">
        <property type="entry name" value="CUE_N4BP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056719">
        <property type="entry name" value="DUF7817"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056720">
        <property type="entry name" value="DUF7818"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052772">
        <property type="entry name" value="Endo/PolyKinase_Domain-Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041801">
        <property type="entry name" value="N4BP2_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535:SF1">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25125">
        <property type="entry name" value="DUF7817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25126">
        <property type="entry name" value="DUF7818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="38"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="770"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="778"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="789"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="853"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="884"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921545.1"/>
        </source>
    </evidence>
    <sequence length="1333" mass="148485" checksum="C9B3A141D7113F91" modified="2020-10-07" version="1">MPKKKKNLGVSPSRKNTNPETICATNASSSPASLHEINKEKLFSGLSEMFSDLDPTVIYMMLSECDFRVEETMDYLLELSTAAKDTVCPSKASGFDSLSALLVGENNSSRSTHELEGNDTTAIAKPGKESQHPSSSEELAPLIENSLEGGSRKSEAEESEKDRLSGSLTLAWDHNNSNCTELGIESSGTAIENWFPALKPSYNEAAESTSISEIETKDILTQTPENCSLPEVVLDSGAVSEMVTLADKGESYTEPHQMHNVLFDFKAVSTLTTQHHQVFATIETNAFSSSHASSRAAEGQENIVTFCHNLKQFCVPDLCTGSGAKSAPLSKETDPSRRTWSSPFSLFQKYPQQLNFYPAFESQCRNHSFVTPIAISPGKWRPALDGRSQGKSFCSPEMSTSWDVVPKRYGNKNGRERHHFPQVPQLPGGHITNRKTFAGHVLVLLRGLPGSGKSYLARVLLEDNCGGIILSTDDYFCQKNGHYQFDADCLADAHEWNWRRAKDAFEKRITPIIIDNTNTQAWEMKPYIALSQQHKYKVIFREPDTWWKFKPKELERRNVHGVSKEKIKRMLERYEHCLTANSVLNSSISDDMRKRETSGEVLHREKRQGKEEQEEHFASPLKSVELNPDEKATLEEVMADDQSLQNENRRRARHDLQESSSECRIDGLDAAVLPAGIKMESILEAELRPISDSSENTLSCNNSELKLQMEATAYHEITEPEFTETQHEDGDLNSGRSIQPKMLNFVGDWPVEQTLGQRVKRIKRLEKRARKDKGDLSTPISTLDSFKNTNEDKASDWIDSHQDLPLSEDRGEDKCEESNCNFPSTSVPEVNAEVHTTELLMVGDWPVQTLQQRQHKMKRKTKRDISESGGLGNSQDDVSMGDGTATSQLNETSADVEEQGASSKEERIPGFEITASEPLGEKKPVPNKRTRKHHKLALTFTNNSALHKPGEPPCTWAEEKPNPWVVSQATKSSQTEPRDFALLWRLEREIVFSEDTKVLHGRLDGFVPKRVDAVPDCPDKIPYKVTYERSTYVEERELVRVDESENLNILCKLFGSLSFDAIKDLYERCNRDMDWATGLLLDSAEKLCKEDDGGALQETGVRLPDVSLPSKRHAAHEDRFAGSVAVPQPTAVSRIIHQSEIAKVPLGDDSENVSGHPTGHGIHFSSGENGGPLNGQTEQGIFRGLSENEPQRTSLVNEMDVAVPDEVDGPLKATCDGEEDQLGANFQEAPFPEEILDLKLMEATDSSVAESHTFEPCRKMDCKPGIVVPTQREKNSPDQDNGAIQLQDSVSHSKSVTIDCLELVLAPEFAMQLSEIFGPVGVDAGNNITDYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC74</accession>
    <name>A0A6I9YC74_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Serine protease 27-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106548768</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921690.1">
        <property type="nucleotide sequence ID" value="XM_014066215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC74"/>
    <dbReference type="GeneID" id="106548768"/>
    <dbReference type="KEGG" id="tsr:106548768"/>
    <dbReference type="OrthoDB" id="9425590at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035821">
        <property type="term" value="P:modulation of process of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000039">
        <property type="entry name" value="Brain-specific serine protease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF144">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE PROTEASE CTRL-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027000244" evidence="10">
        <location>
            <begin position="23"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="37"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921690.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="34659" checksum="A2CF6F6F86A948B3" modified="2020-10-07" version="1">MVRFLGIESLTLLSLLMGITEAQPPSACGRFIPSSRIVGGSVAEVGSWPWQASVQILESHHCGGTLIASRWVLSAAHCFASLSDPSLFAILLGSYNLSNPGLESVLVKVKQIIIHSNYTELIKGQDIALIELETPVNFTQHIQPACLPGSSIVFPPRLGCWVAGWGNIGPQVPLPEPELLQEVLLPLIDRATCETLYSDYKKPGIVEVIKDDMICAGYMEGGKDACQGDSGGPLLCPWNGDWVLAGVVSWGDVCAAPKRPGIYIRISAHTSWILRHAPEVKSSFINALNLTTPCNGCSLVAFANIAMLLSLINNIIAIIFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCI9</accession>
    <name>A0A6I9YCI9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">SLIT and NTRK-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SLITRK2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLITRK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921868.1">
        <property type="nucleotide sequence ID" value="XM_014066393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCI9"/>
    <dbReference type="GeneID" id="106548895"/>
    <dbReference type="KEGG" id="tsr:106548895"/>
    <dbReference type="CTD" id="84631"/>
    <dbReference type="OrthoDB" id="9439679at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000001">
        <property type="entry name" value="SLIT and NTRK-like family, member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773:SF4">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026831048" evidence="11">
        <location>
            <begin position="23"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="633"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="12">
        <location>
            <begin position="218"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="12">
        <location>
            <begin position="534"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="267"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013921868.1"/>
        </source>
    </evidence>
    <sequence length="858" mass="96983" checksum="80FECDEDE522B2EE" modified="2020-10-07" version="1">MQKGVWLLSVFTVAGIAPTTESRKPAKDICSKSRCPCEEKENVLNINCENKGFTTVGLLLPPPSKIYQLFLNGNAFARLYPNEFVNYSNAVTLHLGYNDMEEIRTGAFLGLRILKRLHLNNNKLEILREDTFLGLESLEYLQADYNYIAAIEAGAFSKLNKLKVLILNDNLLLSLPSNVFRFVLLTHLDLRGNRLKMMPFAGVLEHIGGIMEIQLEENPWNCTCDLLPLKAWLDTITVFVGEIVCETPFRLHGKDVTQLTRQDLCPRKGVGEANPREKYPPHSDTHLQRFPPTVNSAVGATRAPKASRPPKTRNRPTPRVTVSKDRQIFGPIMVYQTKSPVPLTCPNLCTCTSQSSDNGLNVNCQEKRIGNISDLHPKPTSPKKLYLTSNYLQTIYKSDLVDYPSLDLLHLGNNRIAVIQEGAFSNLTSLRRLYLNGNYLEVLYPSMFEGLHSLQYLYLEYNVIKEILPRTFDALGNLHLLFLNNNLLRSLPDHVFGGTTLTRLNLRNNHFSHLPVQGVLDQLSALIQIDLQENPWDCTCDIMGLRYWIEQVTEQNNQQSGSPVVINEVVCDSPAKHAGEHLKTLSKEVICPENPNLLDSSLLSLNLNTDMPSAFSIFPSAFPEMRTEVPLSVLILGLLVVFILSVCFGAGLFVFVLKRRKGLPSVPSSANNLDVSSFQLQYGCYNSEAHEKAEGHVYNYIPPPVGEMCQNPIYMQKEGDPVAYYRNLHEFNYSNLEPKKDDSTSFAFTITAADMLEKQSFSREPELLYQNLVERVKELPSGSMVQYNFCTLPKRQFAPSYESRRQNQDRLNKTVLYGTPRKYFTELSKPDLPLLHGKLQAEPDYLEVLEKQTAISQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCL1</accession>
    <name>A0A6I9YCL1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Platelet-activating factor acetylhydrolase IB subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PAFAH1B1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921901.1">
        <property type="nucleotide sequence ID" value="XM_014066426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCL1"/>
    <dbReference type="GeneID" id="106548927"/>
    <dbReference type="KEGG" id="tsr:106548927"/>
    <dbReference type="CTD" id="5048"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000038">
        <property type="entry name" value="Lissencephaly-1 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="39"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="169"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="273"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921901.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="39552" checksum="C706872144D5FB2E" modified="2020-10-07" version="1">MELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGKELKSCSHLNCSQTTRFIGSILCSNLVLMRHVFFPVNSYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSTILEATGSETKKSGKPGPFLLSGSRDKTIKMWDISTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCS1</accession>
    <name>A0A6I9YCS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RPS5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921835.1">
        <property type="nucleotide sequence ID" value="XM_014066360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCS1"/>
    <dbReference type="GeneID" id="106548870"/>
    <dbReference type="KEGG" id="tsr:106548870"/>
    <dbReference type="CTD" id="6193"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8 11" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="9">
        <location>
            <begin position="45"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921835.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="22778" checksum="3DC6C094C7921225" modified="2020-10-07" version="1">MTDWETAPAVAETPDIKLFGKWSTDDVQISDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIIKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDS1</accession>
    <name>A0A6I9YDS1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C1orf194 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922318.1">
        <property type="nucleotide sequence ID" value="XM_014066843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDS1"/>
    <dbReference type="GeneID" id="106549244"/>
    <dbReference type="KEGG" id="tsr:106549244"/>
    <dbReference type="CTD" id="127003"/>
    <dbReference type="OrthoDB" id="10013535at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022179">
        <property type="entry name" value="CFAP276"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12494">
        <property type="entry name" value="DUF3695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922318.1"/>
        </source>
    </evidence>
    <sequence length="169" mass="19257" checksum="619F9DD3B5CF9454" modified="2020-10-07" version="1">MSPARNSFPFPHYENENTYSDNMNAQNTSYGMPSHLSQQQDTWSRLHGTATLASIRREVWYSDSEISKDSLDFNLSSLYNHHEDLLKNKSQVVLHKETLLDDHGTLKVLEPVKKYTSVQHPTSKGITVRHWVNPVKESIHHIEGAIEAPHTAATNSGYSRKENGGIFYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFB0</accession>
    <name>A0A6I9YFB0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 391-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106549684</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922866.1">
        <property type="nucleotide sequence ID" value="XM_014067391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFB0"/>
    <dbReference type="GeneID" id="106549684"/>
    <dbReference type="KEGG" id="tsr:106549684"/>
    <dbReference type="OrthoDB" id="9870178at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000862">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF423">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013922866.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="15114" checksum="7EA718CD39557F8A" modified="2020-10-07" version="1">MEFGKSLSESSDINCHQRIDREDRPYKCMVCEKRIHTGEKPFQCTECGKSFAWTGQLNSHKRTHTKERPYKCIQCGKAFTHKNSLTCHERIHTGEKPFQCTECGKSFAWTGQLNSHKRTHTKERPYKCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFG6</accession>
    <name>A0A6I9YFG6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Olfactory receptor 2D3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106549771</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922983.1">
        <property type="nucleotide sequence ID" value="XM_014067508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFG6"/>
    <dbReference type="GeneID" id="106549771"/>
    <dbReference type="KEGG" id="tsr:106549771"/>
    <dbReference type="OrthoDB" id="10005336at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013922983.1"/>
        </source>
    </evidence>
    <sequence length="266" mass="29732" checksum="53566B3A1B2DFC0E" modified="2020-10-07" version="1">MVVASHLSSPVYFFLNHLAVIDICLSSVTVPKLLGILTARQTISYSACFGQVFFILLTGCNGACILAVMAYDRFAAICKPLHYVQIMKKACCRWLVGGAWTIAFSYAFVNTFSALKLNFCRENFIKHFSCELPSLLALSCTDTLSNWILFFITGGVIVICSFGFILFSYIHIISTILKLNSAEAKRKTFSTCTAHLIVLVLYYSTGCFRYMRPNSASSIIVDELISIQYSISTPMLNPLIYSLKNREVKEAIKKLLGYKPLISHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFT2</accession>
    <name>A0A6I9YFT2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MED12</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922859.1">
        <property type="nucleotide sequence ID" value="XM_014067384.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549678"/>
    <dbReference type="KEGG" id="tsr:106549678"/>
    <dbReference type="CTD" id="9968"/>
    <dbReference type="OrthoDB" id="20828at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019035">
        <property type="entry name" value="Mediator_Med12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021989">
        <property type="entry name" value="Mediator_Med12_catenin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021990">
        <property type="entry name" value="Mediator_Med12_LCEWAV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09497">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12145">
        <property type="entry name" value="Med12-LCEWAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12144">
        <property type="entry name" value="Med12-PQL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01281">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med12" evidence="9">
        <location>
            <begin position="101"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="629"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1709"/>
            <end position="1780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2000"/>
            <end position="2035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="640"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1731"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1745"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1759"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2000"/>
            <end position="2018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922859.1"/>
        </source>
    </evidence>
    <sequence length="2174" mass="241840" checksum="FBB82687BEB966BB" modified="2020-10-07" version="1">MAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWPVTARSQSAINNWFNDLAGTKPLTHLAKKVPIFSKKEEIFSYLAKYAVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFSEWTQIITKFLWEQLQKIADFHRQLLAQACGSPSSIMPQEVEHSVKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLQYSSEFVQSAYLSRRLAYFCTRRLAILLDASGGHPAHHLPSQAAPALPSTPTPQPAAGNSSASPFSDLLLCPQHRPVVFGLSCILQSIVLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGVNASFIQQVRARLRETEQQIKERGQAVEVRWSFDKCQEATAGFTISRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGGGPSKDSHEISPDDDAVVALLCEWAVSHKRSGRHRAMVVAKLLEKRQSEIEAERCGDSEVVDEKGSISSGSLSAVSAPVFQEVLMQFLDTQAPMLTDPSSEDERAEFFNLVLLFCELIRHDVFSPNMYMCALISRGDLAIDHGPRPASPFDDAAEEHHDRKEREGGHGIKLEDASLSECHMDIDHSSNAIFDDMEKTDFSMFSPSMHCESKASPSPEKVDLEKDRGADGTLAALYDQPRHIQYCTHFPIPQEESSSHECNQRLVVLFGVGKQRDEARHAIKRISKDILKVLNRKSTAETGGEEGQKRKRNKPEAFPTAEDIFAKFQHLSHFDQHLVTSQVSRNVLEQITSFALGMSYHLPLVQHIQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPLFMIDTIENPSAHNFTYINLGKSLADNPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKLLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDSSKPSVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLLDDDKKPAGRMVSIETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSLLELQLMIKQTASNQEMNSLLENIAKATIEVFQQSAETNGIVSLGSCPNLMPANPKAKPVLSSLERSGVWLVAPLIAKLPTSVQGYVLKAAGDELEKGQHLGSSSRKERDRQKQKSMTLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSESLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRKVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKVVEPAKVEKASASAGSSAAEPKNKSKSKSKKRAQPASKNEEYPMNAVRGVSYGAGIPNDLLHHQPGNSMSRLAYGQPPVGLYAQNQPLPAGGPRLDTSYRPVRMPIGKLVPNRLPYAGVLPTGMSMLGMDPSYKSAVYRPQQPPPPLSGGTLLRQQLQAKLQGQGILGQPPVRQMPPTPAYGTGQAAQGYTPYGVPHLSLQQHPSQASAMVPSTYSGQTYQNSHPSANPAFVDAVRSMQRPGYVHQQAPAYGHALNAAQRFPHQPMQQPTMMGSINHLSTQGVPTGVRPSQLLPDQQQQQQQQQQQQQQQQQQQQQQYLRQQQQQQQQLRANGTIFAHPGKLAAAVSAAGGPGGQNLLACGSLSSEDQRPNEVTSMMPFWLLPNASTEFKGCEGNPSASRHVGCKRASLSLGVLWGVQTFPRIASKRDRRRGMSLKPCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGD9</accession>
    <name>A0A6I9YGD9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger and SCAN domain-containing protein 31-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106549996</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923242.1">
        <property type="nucleotide sequence ID" value="XM_014067767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGD9"/>
    <dbReference type="GeneID" id="106549996"/>
    <dbReference type="KEGG" id="tsr:106549996"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000028">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="15">
        <location>
            <begin position="165"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="382"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="465"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="549"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="577"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="296"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923242.1"/>
        </source>
    </evidence>
    <sequence length="628" mass="71989" checksum="15F4535FDF3242DE" modified="2020-10-07" version="1">METLSVQVNMSMEEESMEHPEPKMPKQYTSLVQLWADMEARKAPSRRVPRQQVKCKPEEGLQDCWEAQWQEFLGSLQGPRSERAHAETVEEPMPWANTKAFLTSFEQVASACKWPQDQWATLLAPGLRQEAAEAFNSLSGQERRDYGKVKAAILRREALAREKQRRDFREFCYQETEGPRALYKHLQGLCWQWLKAEKCSKEEILELLVLEQFLTVLPPEMQSWVRGHGPETCTGAVLLAEEFRWKGSSVKQEEESPGPLEMLAVEEPFQGSQFLPLGSWEWQLPKEMKLDIEEEMSSFDDDDDRQTNQDNHLPQSISEAPENEILKRDIIPSQRCKEEVRPKNQEDPEAELDSSKGSLDEVVSVGKSDSDPEEAKTSHKIHECHCGKRFRWSSNFRAHERIHTGEKAYTCTECGIPFRKSAQLKAHKGIHTGEVPFQCSICQKTFTSSSNLITHKRIHTGEKPYRCTYCGKSFRQKGTLTIHEKIHMDEKPFKCLTCGKTFRQKGTLSVHEKIHLGEKPYRCSSCGKNFRTAAVLRVHERIHTGVKPYQCSVCLKSFTDGSNLITHERIHTGVKPYQCARCGKTFCQKSGLMTHERIHTGVNPYKPSADQNHLEGTFDLDAHQKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGG8</accession>
    <name>A0A6I9YGG8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106550017</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923272.1">
        <property type="nucleotide sequence ID" value="XM_014067797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGG8"/>
    <dbReference type="GeneID" id="106550017"/>
    <dbReference type="KEGG" id="tsr:106550017"/>
    <dbReference type="OrthoDB" id="10265871at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056362">
        <property type="entry name" value="AtuA-like_ferredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010839">
        <property type="entry name" value="AtuA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47708">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47708:SF2">
        <property type="entry name" value="SI:CH73-132F6.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07287">
        <property type="entry name" value="AtuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23544">
        <property type="entry name" value="AtuA_ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyclic terpene utilisation N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="AtuA-like ferredoxin-fold" evidence="2">
        <location>
            <begin position="168"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013923272.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="31667" checksum="2DBFAF6B3A4E38BD" modified="2020-10-07" version="1">MGGPRAIEKGKRMAESILERTRIMFHQRGYEDYSAVNLQILGSEETYGPHAKQNIDGGPREVVIWLAVFHKQKEALEIFTREIAPAGTGMAPGVTAIIGGRPKISPVLKPFFFLYPKKYIKINIFMNGQHVETFQEDISFTSDLTASHDEPQSQEEFKDLPSGPHVYRLEDLAYTRSGDKGNTANIGVIARHPLYYPYLKKTLTALAVENYFQHLLEREQPEEILVSRYDLPGIYGLNFVLKNSLGGGGTASLRSDPQGKAFGQMLLDFKIKHVPDLKSLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH88</accession>
    <name>A0A6I9YH88_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sphingomyelin phosphodiesterase 2</fullName>
            <ecNumber evidence="6">3.1.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Lyso-platelet-activating factor-phospholipase C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Neutral sphingomyelinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">SMPD2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36087"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:34115"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:36088"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-O-octadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-octadecyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74001"/>
            <dbReference type="ChEBI" id="CHEBI:75216"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:39924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:75542"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:41120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a sphingosylphosphocholine + H2O = a sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:84410"/>
            <dbReference type="ChEBI" id="CHEBI:85171"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:45297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an N-(acyl)-sphingosylphosphocholine + H2O = an N-acyl-sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45300"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64583"/>
            <dbReference type="ChEBI" id="CHEBI:83273"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:45301"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013923547.1">
        <property type="nucleotide sequence ID" value="XM_014068072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH88"/>
    <dbReference type="GeneID" id="106550208"/>
    <dbReference type="KEGG" id="tsr:106550208"/>
    <dbReference type="CTD" id="6610"/>
    <dbReference type="OrthoDB" id="387657at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000033">
        <property type="entry name" value="sphingomyelin phosphodiesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="24">
        <location>
            <begin position="25"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013923547.1"/>
        </source>
    </evidence>
    <sequence length="406" mass="46079" checksum="3B6044A7054B03B3" modified="2020-10-07" version="1">MEDSLVQLRIFDLNCWAIRYLSKLRRERVALIGDVLNQEGFDLALLQEVWSEKDYLMLQKKLCIHYPSSHYFKSGVIGSGLCIFSKHQILDTFLYQYSVNGYPYMFQHGDWFAGKSVGLIVVKIQGIIFNVYVTHLHAEYSREKDTYLPHRVVQAWELGQFIQHTSKGADVVLLGGDLNMHPEDIGIRLLRGWTGLQDSFIVAEKIKGCKDGCTLVPANCYINKKELQCYPQGIRIDYVLYKGSSQYDIRCKIHETTTGTAPGKDFPYSDHEAVKVTLSVVQSNKSENNLPVEPVLVDVLNEARLEVQVGLHSAKRQQYFCGRMAVLALFLLVIQGTMAMSSMFGGALQQTFPKFSFSLLNLLSVSVLLISAVLYLFHIIEVKVLQGTEEQMRVGLQTLQDKFNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YH89</accession>
    <name>A0A6I9YH89_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">G patch domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923339.1">
        <property type="nucleotide sequence ID" value="XM_014067864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YH89"/>
    <dbReference type="GeneID" id="106550078"/>
    <dbReference type="KEGG" id="tsr:106550078"/>
    <dbReference type="OrthoDB" id="20507at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13384">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13384:SF19">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923339.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="43531" checksum="0A5559A2D845FDCD" modified="2020-10-07" version="1">MTEWERGREREEFSRAAAFYKTSSSILSSRFTPAKLEDDTDKVDVPRDEETNVDDKEAAAKMKMFGKLTREKFEWHPDKLLCKRFNIPDPYAESCIVGLPTVKRDKYSVFNFLTVASESASEMLNQPMKTGVQQDNIPNKPKKSSRWDVPDREKKKDSISEFISLARSKVDVHAQDQLPKAVEAKAKEGTGDLVPTQPSGPPSGPSEAAVEEESRPSMDLFKAIFAGSSDEKSSSEEESDGEEPAATALAGESENAKPCDSPGVPLPVAQETEAIPETFDTITPTLKEVVDQGEFGPRLPPVAYPGTAEVVAEHKTPKIKEKHKKKEHKHKKEKKKKHKKSKHKGKHKNKKSEKNSSSDTAESRDSSSEEGKTNISPRELLRRMKNLVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YI00</accession>
    <name>A0A6I9YI00_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger and BTB domain-containing protein 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106550582</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923993.1">
        <property type="nucleotide sequence ID" value="XM_014068518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YI00"/>
    <dbReference type="GeneID" id="106550582"/>
    <dbReference type="KEGG" id="tsr:106550582"/>
    <dbReference type="OrthoDB" id="1405595at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000620">
        <property type="entry name" value="Zinc finger protein 263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF29">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="33"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="136"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923993.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="48318" checksum="B0CFDAABC11532FD" modified="2020-10-07" version="1">MAPASERLQFRFPGYGDGVLHRMDQLREQRRFCDVTVQVNELRVPGHRVVFAACSPFLRDQFLLNDSQEVTVSLFQSPEIGRQLLLSCYTGCLDVPIKELVNYLTAASFLQMGHVVERCAQAVSHYLVPKADALLQEKQEEESSEQQDGEEEEEEEEEREKQPSSTPGKADCEESKMAAVEFTPRSSHGPASPAISLSLINSAVEITRSYLQGCYDDEMGSKGLPFPSATPSPASQWRNYPLHRHRPAMNYREWRTGSGVASWKVPSLERPYRCPRCSRVFQQLGHLVSHVQEHKLFLCLRCGKVFSQKSNLTRHIRVHTGFKPFQCPICRKCFTQNATLQDHLNLHSGIKPHKCNYCEMHFTHKPGLRRHLKELHGKSTVQNSHEEIEEVTIDFDVRGGHAEGGYSLNGRFFCEPKGGLPAPASSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIS5</accession>
    <name>A0A6I9YIS5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein-tyrosine sulfotransferase</fullName>
            <ecNumber evidence="13">2.8.2.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">TPST2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16801"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:11688"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:65286"/>
            <dbReference type="EC" id="2.8.2.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the protein sulfotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.20" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013923720.1">
        <property type="nucleotide sequence ID" value="XM_014068245.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923722.1">
        <property type="nucleotide sequence ID" value="XM_014068247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIS5"/>
    <dbReference type="GeneID" id="106550348"/>
    <dbReference type="KEGG" id="tsr:106550348"/>
    <dbReference type="CTD" id="8459"/>
    <dbReference type="OrthoDB" id="545675at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008476">
        <property type="term" value="F:protein-tyrosine sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000290">
        <property type="entry name" value="Protein-tyrosine sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026634">
        <property type="entry name" value="TPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013923720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013923722.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="41917" checksum="0A97692AC3EF68F2" modified="2020-10-07" version="1">MRLTVRRVLMGVGFAIALMVSVHLGQQMLQCQQMLGQGLSRALMRPESEELVMLDSNRVEYRYSKEMPLIFIGGVPRSGTTLMRAMLDAHPEVRCGEETRIIPRVLAMRQAWSRSGREKMRLDEAGVTDQVLDAAMQAFILEVIAKHGEPARYLCNKDPFTLKSSIYLSHLFPNSKFLLMVRDGRASVHSMITRKVTIAGFDLRSYRDCLGKWNKAIEVMYSQCLAIGRLRCLPVYYEQLVLHPQKSMRAIMDFLGIAWSDAVLHHEELIGKPGGVSLSKIERSTDQVIKPVNMEALTKWLGHIPEDVVQEMAQIAPMLARLGYDPYANPPNYGSPDPMVINNTHRVLNGDFKTPANLKGHLQGTLNTTAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ02</accession>
    <name>A0A6I9YJ02_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">[histone H3]-lysine(4) N-methyltransferase</fullName>
            <ecNumber evidence="17">2.1.1.364</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">KMT2B</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60264"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.364"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:60265"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.364" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013924217.1">
        <property type="nucleotide sequence ID" value="XM_014068742.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106550780"/>
    <dbReference type="KEGG" id="tsr:106550780"/>
    <dbReference type="CTD" id="9757"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035097">
        <property type="term" value="C:histone methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140945">
        <property type="term" value="F:histone H3K4 monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15694">
        <property type="entry name" value="ePHD_KMT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15589">
        <property type="entry name" value="PHD1_KMT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15593">
        <property type="entry name" value="PHD3_KMT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19170">
        <property type="entry name" value="SET_KMT2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000004">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.360:FF:000006">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000071">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000089">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001155">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.360">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003888">
        <property type="entry name" value="FYrich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047219">
        <property type="entry name" value="KMT2A_2B_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041959">
        <property type="entry name" value="KMT2B_ePHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45838:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45838">
        <property type="entry name" value="HISTONE-LYSINE-N-METHYLTRANSFERASE 2 KMT2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05965">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05964">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00542">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00541">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
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        <property type="entry name" value="ZF_CXXC"/>
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        <property type="entry name" value="ZF_PHD_2"/>
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    </dbReference>
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    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CXXC-type" evidence="24">
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013924217.1"/>
        </source>
    </evidence>
    <sequence length="2647" mass="291082" checksum="E52196BDCE6E17A5" modified="2020-10-07" version="1">MVKALAELLQRTKGTPGRIGRQASSGPKVLKVLPKQTQADPEPADPLKTTAPPLLPPPTEKTLNSTASPRPRGRPRGSFRKKPGTRPPPKSTSTSSSIPEPNNSIQLPSSCAEEISASSEPSEALKKPPLILKFFSKARRHKSGQLVVTHVRLKTHGHRRGRKRKWSVVPEKKLSPLPSEAVSESEAVLSEPDTSDPEPKQKESPAPHLPSENPAPEASPRPKSPVSKPEPQLEDVVQELQPAPETLVPEPDTSIPEPKPSVPEGDAVPSPNQVSWQQEPQQPCPSSDGAAFLSEKGPSRVALSIRARCLKRGRGRRAATQPLPGKVREDAGGTAQQSGVAGSEEVPALKTTFLKNIRQFIMPVVSARSSRLIRTPRRFMDEIPQKATKLLESPVPDGALDKQEASLLPLQAPTQQLNPSVPGSPEKDPSRAPSSPSSPGGLHITSPASALREKRRSILREPTFRWTSLSPTLSPKDMGKALFQVPSEEASLVPSAPAPVPSTPTKRTPLLRAPQFTPSEAHLKIYESLSVSPEESDLVPASPEVRREPPVPLQEATLLSSEPVVTRSGKKLVGRTNHLALPHFTEVPKPLEAQSTELITMEDVNSPGVVHKLAIRRVVPPSPQVEEQEEKAADSSESEAEPASLSSEEAKPPVQQPKPIVTSLSSMDKVYSLLTRAKVQLYKIDQQKQFKFIPGCQSIKAEAFETPKIPAEQETPEDLNTTEDKKEVRKQGQDSPVQGPRIKHVCRHASVALGQSRAMVPEDVPRLSALPLREREEIAGSPTIEETSSASENECGQQKQVKPDSAVKATPPPVRRHVSHHHGKKNRMTRCGKCKGCLRLQDCGECINCLDKPKFGGPNTKKQCCVYRKCDKIEARRVERLSKKGRTVVKPIAPWDSEDSQEEFPAPWEVMLGATETAEQDSLLQRKSARRCVKQRPSYDIFESSDSDTEPASGSATHRRKSSRDSDLLPVDSEEQSRPRKSSLQPVVQLKARKKPEKDIPPSASLPSISNGWNGKQKSTDGVHRLRVDFKEDCDLENVWLMGGLSILTSVPVTTQVVCLLCASKGFHQLVYCQVCCDPFHVFCLEEDEQPLPEQEDSWCCRRCKFCYVCDRKNKASKQLLECERCRNCYHLACLGPNYPTKPFRKRKGWICSACIRCKSCGTAPGKNWDTEWSSDYSLCSACSVLYNKGNYCPICLHCYEDNDYESKMMQCANCDHWVHAKCEGLSDEGYEILSNLPESVVYACRPCCGSDKTKWREVLHSELRKGLRQVLQGLLSSKCVAPLMQCAQCCPDDGVKVHLRPCDLRTVNKLFEQGHYSSVHSFNEDIVGVLLGQTEEEEVTFTAKELYIELMGKYFSWFDALDAKHWTRNTSLPNGMLPNAVLPPSSDHIYAQWRQPQEISSTHNSHLSSVGPENIAGKDPEHVPPQQLLADPEDKRQCALCLQFGDAPSKDAGRLLYIGQNEWTHVNCAIWSAEVFEENDGSLKNVHAAVARGRQMRCEHCQRTGATVGCCLSACLSNYHFMCARLCHCTFQEDKKVFCQKHVDLLDGTEIVAEDGFDVLRRVYVDFEGISFKRKFMVGLEPDTIHMMIGSMKIDSLGMLTDLSECEGRLLPVGYQCSRLYWSTVDARKRCGYTCRVMEYRPKLSQEEPDASGMQEENHTIVHSPAAPLAPNLRDKPLQEAPSPVPSLERHSPAQNPGPLPPPEPAVSKPKPLTGARIKVPNYSPTRRPLSGVSSRPLPSPGSASSMSHHILTVGDPDFPPPRRTQRLSPMSPSSNPRPRRSPLPPQAPASRTSPPSTSLRAMPPLPDYPTAGNGFRHPSTSAGCDEQRPVATSPPPSSPPVPPELAFELNVSDLEFDDSLLDEPFQEEELGGPRTCPPSPNLGLPQLDGLGDGESEEEGEADRYFRFPRTVVTRDPPLPPYPLDLPLPHIHQLDGIDDGTDSEADATGGHPSTKGKQPEPRVERELAGPTPAPEDLPSDIVDFVLKNMDAPESKASPPTCLSPMPLPPAPPTSASLNGTEPAHPSPAPDPAPTLTLGCAQVPPIPEVTTVLAPEAQKPNSRIILVNKLAQVCMKVQGEESPLATQDGEQAFKPNPTPISAAPNLGTVILRAPLGLAPNPLPTWTIRGPVLSMVPMMNVVGTTSQAVGGGQLALSTPALVTPSLGLPQTCLLQPVAMNSGVLSIPGLVSLPGPRPAIRVKRVSTFVGNVPVKKIKMDGVNDEEPPPPVSPTVTPDHLANNCISGSIIGPGIGRVRMKTPTTKGVLNLDAFKEETSIVSESEQSPMEHSPSLAALHSHPGSPEQIQKSVLEHVWHRYAGDLSSSDEDNLKQEDKENEVPRKTQPHLCFEITSEDGFKVQADSIDGAWKAVIEKVQEARTNARLKHLSFAGMNGVRLLGMHHDAVIFLVEQLYGAKACHKYKFRYHQHEGEEEELPLNPHGCARAEVYVRKCTFDMFNFLASQHRVLPDGGPYDEEEDEVQLKSTRRATSLELPMAMRFRHLKKTSKEAVGVYRSAIHGRGLFCKRNIDAGEMVIEYSGIVIRSVLTDKREKYYDSKGIGCYMFRIDDFDVVDATMHGNAARFINHSCEPNCYSRVIHVEGQKHIVIFALRRIFRGEELTYDYKFPFEDAGSKLPCNCGAKRCRRFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ67</accession>
    <name>A0A6I9YJ67_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">Syncoilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8" type="primary">SYNC</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7 8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924251.1">
        <property type="nucleotide sequence ID" value="XM_014068776.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924252.1">
        <property type="nucleotide sequence ID" value="XM_014068777.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924253.1">
        <property type="nucleotide sequence ID" value="XM_014068778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ67"/>
    <dbReference type="GeneID" id="106550803"/>
    <dbReference type="KEGG" id="tsr:106550803"/>
    <dbReference type="CTD" id="81493"/>
    <dbReference type="OrthoDB" id="8842296at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027702">
        <property type="entry name" value="Syncoilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47147">
        <property type="entry name" value="SYNCOILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47147:SF1">
        <property type="entry name" value="SYNCOILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="4">
        <location>
            <begin position="75"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="282"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013924251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013924252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013924253.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="45115" checksum="A9F8B76592DDF515" modified="2020-10-07" version="1">MADFELPVEQNSEETLEPNIAGVGTLPEFNQENVPEFFYRDPISAQFQPNPEITGASLGPQESMTCQDLSTAESQHVEVEDLGDRFQQCIEAVEQLEEERDCLIKELTLLRQPALKELQRAHEEILEAYRLHAKVELEKDNLWDEVRQVKQKLFRVTRECVACQYQLETRRYEVTQHVDYRKELENRVSQLSEELSQLRETCEEEKEHFRQRLKTPRHERSSRHLQESRRLSMEFESFVLENRQCLEEHYEPKLMRLLERREASSKALQTTQSEIHRLKETLRPLQGEVSKLLLQNRNLEEQILLIKQKRDEEVLQYKEQVEEMENRMRELKTGVQLQQRKNQELEELKASLHQELSIYKGCLEMYGQLCKSEVKEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLQ8</accession>
    <name>A0A6I9YLQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">GalNAc alpha-2,6-sialyltransferase VI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">ST6GalNAc VI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Sialyltransferase 7F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ST6GALNAC6</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-O-[alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-alpha-D-GalNAc]-L-Ser-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-{alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-[alpha-Neu5Ac-(2-&gt;6)]-alpha-D-GalNAc}-L-seryl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65280"/>
            <dbReference type="Rhea" id="RHEA-COMP:16760"/>
            <dbReference type="Rhea" id="RHEA-COMP:16761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:156395"/>
            <dbReference type="ChEBI" id="CHEBI:156397"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:65281"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3-O-[alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-alpha-D-GalNAc]-L-Thr-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-{alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-[alpha-Neu5Ac-(2-&gt;6)]-alpha-D-GalNAc}-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65284"/>
            <dbReference type="Rhea" id="RHEA-COMP:16762"/>
            <dbReference type="Rhea" id="RHEA-COMP:16763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:156396"/>
            <dbReference type="ChEBI" id="CHEBI:156398"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:65285"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-acetyl-alpha-neuraminosyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-N-acyl-sphing-4-enine + CMP-N-acetyl-beta-neuraminate = N-acetyl-alpha-neuraminosyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-[N-acetyl-alpha-neuraminosyl-(2-&gt;6)]-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-N-acyl-sphing-4-enine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47884"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:88073"/>
            <dbReference type="ChEBI" id="CHEBI:88079"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:47885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a ganglioside GD1a (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GT1aalpha (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41972"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78445"/>
            <dbReference type="ChEBI" id="CHEBI:78571"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:41973"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a ganglioside GM1b (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD1alpha (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41968"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78568"/>
            <dbReference type="ChEBI" id="CHEBI:78569"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:41969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a ganglioside GT1b (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GQ1balpha (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41976"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78452"/>
            <dbReference type="ChEBI" id="CHEBI:78572"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41977"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a globoside MSGG + CMP-N-acetyl-beta-neuraminate = a globoside DSGG + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:140623"/>
            <dbReference type="ChEBI" id="CHEBI:140624"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:56089"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925167.1">
        <property type="nucleotide sequence ID" value="XM_014069692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLQ8"/>
    <dbReference type="GeneID" id="106551569"/>
    <dbReference type="KEGG" id="tsr:106551569"/>
    <dbReference type="CTD" id="30815"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001665">
        <property type="term" value="F:alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009988">
        <property type="term" value="P:cell-cell recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001574">
        <property type="term" value="P:ganglioside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000009">
        <property type="entry name" value="alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45906">
        <property type="entry name" value="ALPHA-N-ACETYL-NEURAMINYL-2,3-BETA-GALACTOSYL-1, 3-N-ACETYL-GALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45906:SF6">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0730">Sialic acid</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="75"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00074915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00077208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013925167.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33985" checksum="E7104987B897A4CD" modified="2020-10-07" version="1">MKNHQSPHTVVLVILFALFMLLIIYNSNSHNEALLRYTVSQAKPPLPSSLKKWGVKGEYLPVPGGKPLVQHCNECALITSSSHLLGSKLGRAIDESECTIRMNDAPTTGFSDDVGNKTTFRVVAHSSAFRVLKRPQEFINKTPENILIFWGPPLKMQKSIIKLIERVSLSFPNMSAFAVSPKRMQQFDELFRKETGKDRQTSRSWLSTGWFTMVIAVELCDKLHVYGMVPPSYCNKTPPHRLPYHYYEPKGPDECTTYIHNERSHKGNHHRFITEKRVFGRWANLYNITFSHPEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLU8</accession>
    <name>A0A6I9YLU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="12">MAD homolog</shortName>
            <shortName evidence="12">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106551732</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925353.1">
        <property type="nucleotide sequence ID" value="XM_014069878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLU8"/>
    <dbReference type="GeneID" id="106551732"/>
    <dbReference type="KEGG" id="tsr:106551732"/>
    <dbReference type="OrthoDB" id="5794312at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060485">
        <property type="term" value="P:mesenchyme development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10985">
        <property type="entry name" value="MH2_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000007">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF25">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026699640" description="Mothers against decapentaplegic homolog" evidence="14">
        <location>
            <begin position="23"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="MH1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="16">
        <location>
            <begin position="214"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="147"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013925353.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="46263" checksum="99595DC407BA5DF4" modified="2020-10-07" version="1">MLPYSHQLFLFLFFQFIHSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPSTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YML4</accession>
    <name>A0A6I9YML4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable N-acetyltransferase 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NAT16</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925371.1">
        <property type="nucleotide sequence ID" value="XM_014069896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YML4"/>
    <dbReference type="GeneID" id="106551748"/>
    <dbReference type="KEGG" id="tsr:106551748"/>
    <dbReference type="CTD" id="375607"/>
    <dbReference type="OrthoDB" id="8889733at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000039">
        <property type="entry name" value="Probable N-acetyltransferase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056483">
        <property type="entry name" value="Hisat_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47403">
        <property type="entry name" value="LOC100145250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47403:SF3">
        <property type="entry name" value="N-ACETYLTRANSFERASE 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24066">
        <property type="entry name" value="Hisat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="11"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925371.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="35908" checksum="427DCB0DAA817E00" modified="2020-10-07" version="1">MKLESAADSQLEFAIATERDFEEILAISKGIYGGLDYLPSRYHAWIHEPNRTVVLVKKNGVVIGLQSVYIIDAGETALVEGLRVAPWERGKGVAGVLQRFCSAMVKEKHPEVKVSRLTRDDKLGPKDLRKYRIIAKQGILLVRFQAPEVLSRLDAAVLAQGGFSPEPSELLLEKEVPEVVLKEAFRSEVLPNQTIIQDWQPFKATQSNLGLLQKKSLRWVVDRKAHPTVATLSTQPFSIPLGKDWHYLNIDAFGRNPAHLKSQLVHHLRLQVPTLRGGVMCQLFLEPQLWLEMAAFCQEALGLELAKDYTEQYLLEADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN75</accession>
    <name>A0A6I9YN75_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Actin-related protein T2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106551737</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925359.1">
        <property type="nucleotide sequence ID" value="XM_014069884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN75"/>
    <dbReference type="GeneID" id="106551737"/>
    <dbReference type="KEGG" id="tsr:106551737"/>
    <dbReference type="OrthoDB" id="10053773at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925359.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="40486" checksum="34C29AE39433F390" modified="2020-10-07" version="1">MSNVEVLQTPAVIIDNGSGSCKAGLSGEAEPKAIVPSVVGRFQEKPEKIYIGVGAMAKKGALLSYPVERGVISCWDDMEKLLRYIYRYKLKVKPSTRPAFLSEPPKNPLKNREKITELMFESFRVPALYLAIQATLALYASARTTGLVLDSGDGVTHAVPIYDGYCLPHGVSKLPLAGRDVTDYFANLLMEKNPGLVSRAKKTILRRIKENFCYVALDPKQEALKKVEQKVKLPDGNTIKIHLDLCQAPEILFTPKTIGVEAPGIHTMITRSIHKCDKDIWAPLYGNVVLSGGSSLFCGLDERIFKEIEPHVPNGVPIRIITPPERSCAAWVGASVISNLVSFQPMWFTKKDYKEFGVAAIHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPA9</accession>
    <name>A0A6I9YPA9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 22 member 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLC22A15</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925535.1">
        <property type="nucleotide sequence ID" value="XM_014070060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPA9"/>
    <dbReference type="GeneID" id="106551895"/>
    <dbReference type="KEGG" id="tsr:106551895"/>
    <dbReference type="CTD" id="55356"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="22"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="515"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925535.1"/>
        </source>
    </evidence>
    <sequence length="544" mass="60747" checksum="9D8C2C301DA9CBDE" modified="2020-10-07" version="1">MEVEEAFPLVGQMGPFQVWLCVLMAVLLQLYVASEALLIALVGAIPPYHWDLEGILPNHSHSNDTTIEENNFRKWLLTANWNDLHKYVHFNKNFTSIASEWFLIGNTSYKISVASSCFFGGVFVGVITFGQLSDRFGRKKVFLIGFGLDIVFAVANGFSPTYEFFAVSRFLVGIMNGGMSLVAFVLLNECLGTAYWALAGSLGCLFFAVGIAQYALLGYLIRSWRLLALMVNLEGAVVFLLCLFIPESPRWLYSQGRLHEAEDSLYLIAKRNYKPKCTFSLKLPTAWSCKESCSIMDLFRYKILLGRTLVMLYIWFVCSLVYYGLTLNVGDLGGSIYANLALSGLIEIPAYPLCIYLIGQKWFGRKRTLAAFLFLGGLASLLVMFLPQQKATGVFAIVNNRSLSLLGKLTISSAFNIVYIYTSELYPTVIRNVGMGTCSMFSRIGGIIAPFVPSLKVIYWYLPYVVFGVASVLAGFLSLLLPETLNSPLMETLSDIPVCSYRRLEGEAMSLKILEDSQSDQDSSRTESDNEEFYDASEETQMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPN9</accession>
    <name>A0A6I9YPN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Geranylgeranyl transferase type-2 subunit alpha</fullName>
            <ecNumber evidence="3 11">2.5.1.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Geranylgeranyl transferase type II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RABGGTA</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to cysteines occuring in specific C-terminal amino acid sequences.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_013926478.1">
        <property type="nucleotide sequence ID" value="XM_014071003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPN9"/>
    <dbReference type="GeneID" id="106552670"/>
    <dbReference type="KEGG" id="tsr:106552670"/>
    <dbReference type="CTD" id="5875"/>
    <dbReference type="OrthoDB" id="1658at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.120:FF:000001">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.120:FF:000035">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1130:FF:000001">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000138">
        <property type="entry name" value="geranylgeranyl transferase type-2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1130">
        <property type="entry name" value="Rab geranylgeranyltransferase alpha-subunit, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036254">
        <property type="entry name" value="RabGGT_asu_insert-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009087">
        <property type="entry name" value="RabGGT_asu_insert-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF2">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07711">
        <property type="entry name" value="RabGGT_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49594">
        <property type="entry name" value="Rab geranylgeranyltransferase alpha-subunit, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 11" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rab geranylgeranyltransferase alpha subunit insert-domain" evidence="12">
        <location>
            <begin position="271"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013926478.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="68317" checksum="767F85E56F29ECBD" modified="2020-10-07" version="1">MPVGVLEELGKELDTGFILCLFPLESVKMHGRLKVKTTEEQAEAKRLEREKKLQQYVTVTKAIFEKRKLGELDKEALELSNKVLGANPDFATLWNFRRETFLHLEKEESPEEMQALCKGELAFLECCLRVNPKSYGTWHHRCWVMEHMPEPDWARELELCGKFLEIDERNFHCWDYRRFVVQRSKVLPQDELAFSDSLITRNFSNYSSWHYRSLLLPQLYPDPQHQGQITEEILLKELELVQNAFFTDPNDQSAWFYHRWLLGRGDPQPTIRCVYVNRQNTSLAVAFSHPVAVAPASHDLIVFGDESPLVVRWRTPEGKNKPGYMWLCDLPTSALNDHWPQHTFRVLWAEGHVQKECVLFKGHKDCWNQDSVTDEQVFRCELSIEKSTVLQSELESCKELQALEPENKWCLLTIILLMRALDPLVYEQETLRYFSALKAADPMRSSYLNDLWSKFLIENSVLKMEYADSRVVDLAHKGLTSLCHLEHLLLVTHLNLSDNLLSAFPSTVAMMRCLEVMEADDNQIESLEGLPPLPSLEELSLRNNRIQRASALRSLAAFPALAQLSLQGNPLCQTPGIQSELATLLPNIATILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPZ0</accession>
    <name>A0A6I9YPZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Aldehyde dehydrogenase family 3 member A2</fullName>
            <ecNumber evidence="13">1.2.1.3</ecNumber>
            <ecNumber evidence="14">1.2.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Fatty aldehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">ALDH3A2</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="32">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(2E)-hexadecenal + NAD(+) + H2O = (E)-hexadec-2-enoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17585"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2E,6E)-farnesal + NAD(+) + H2O = (2E,6E)-farnesoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24216"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15894"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83276"/>
            <dbReference type="EC" id="1.2.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2,6,10,14-tetramethylpentadecanal + NAD(+) + H2O = 2,6,10,14-tetramethylpentadecanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49189"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:77268"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>22-oxodocosanoate + NAD(+) + H2O = docosanedioate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:39015"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:76298"/>
            <dbReference type="ChEBI" id="CHEBI:76299"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a fatty aldehyde + NAD(+) + H2O = a fatty acid + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:49832"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:35746"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>decanal + NAD(+) + H2O = decanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44104"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27689"/>
            <dbReference type="ChEBI" id="CHEBI:31457"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>dodecanoate + NADH + 2 H(+) = dodecanal + NAD(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:44168"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:27836"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>heptanal + NAD(+) + H2O = heptanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44108"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32362"/>
            <dbReference type="ChEBI" id="CHEBI:34787"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>hexadecanoate + NADH + 2 H(+) = hexadecanal + NAD(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:33739"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17600"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>octadecanal + NAD(+) + H2O = octadecanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17034"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>octanal + NAD(+) + H2O = octanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44100"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17935"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>tetradecanal + NAD(+) + H2O = tetradecanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:84067"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="13"/>
    <dbReference type="EC" id="1.2.1.94" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013926357.1">
        <property type="nucleotide sequence ID" value="XM_014070882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPZ0"/>
    <dbReference type="GeneID" id="106552564"/>
    <dbReference type="KEGG" id="tsr:106552564"/>
    <dbReference type="CTD" id="224"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004028">
        <property type="term" value="F:3-chloroallyl aldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120553">
        <property type="term" value="F:farnesal dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF9">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE FAMILY 3 MEMBER A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="29" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="30">
        <location>
            <begin position="2"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="30">
        <location>
            <begin position="167"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="29">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_013926357.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="35917" checksum="C1FE6EA44E0B5BA3" modified="2020-10-07" version="1">MEKLKRTVDQARAAFNTGKTRPLAFRIQQLKALKKMLEEKEKEFAAALMADLHKSAFNSFSYEFMIILGEIDQMLEKLPDWASPQPVEKTVMTLGDKTYIHREPLGVVLVIGAWNYPFVLVMGPLIAAIAAGNAVVVKPSEVSENTAKLFEKLLPQYLDKGILSSCRRIAWGKYMNCGQTCIAPDYILCSPSIQKTVVEIMKQKLKEFYGENVKDSPDYERIVNKRHFKRLTGLLEGQKVAYGGETDESTCFIGEPPTHTPSTHRCVEGLLQSLFFSLSSQVIDKMIAETSSGGVTANDVLIHFSIPGFPFGGVDQFRIPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQG0</accession>
    <name>A0A6I9YQG0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C-factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106552201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925890.1">
        <property type="nucleotide sequence ID" value="XM_014070415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQG0"/>
    <dbReference type="GeneID" id="106552201"/>
    <dbReference type="KEGG" id="tsr:106552201"/>
    <dbReference type="OrthoDB" id="7289984at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF38">
        <property type="entry name" value="C-FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013925890.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="15944" checksum="6A29EDE866AE3869" modified="2020-10-07" version="1">MLEVYNTNVVGPMVMSQVFLPLLRKASKDSLVKGMSCSKAAIVNMSSEVGSIANMLEWQRGHVIAYRCSKSALNMLSKCQSLCLAEDGILCVALHPGWVQTDMGNKLGPAPLTMEQSLRGILNTLGRLSEENTGTFVNWEGKLVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSS8</accession>
    <name>A0A6I9YSS8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Raftlin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RFTN1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the raftlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927352.1">
        <property type="nucleotide sequence ID" value="XM_014071877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSS8"/>
    <dbReference type="GeneID" id="106553396"/>
    <dbReference type="KEGG" id="tsr:106553396"/>
    <dbReference type="CTD" id="23180"/>
    <dbReference type="OrthoDB" id="9942562at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028169">
        <property type="entry name" value="Raftlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17601:SF3">
        <property type="entry name" value="RAFTLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17601">
        <property type="entry name" value="RAFTLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15250">
        <property type="entry name" value="Raftlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="416"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="503"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927352.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="64830" checksum="46A7F8CFBD8A1238" modified="2020-10-07" version="1">MGCKLNKMEKHDDKRPGNIYSTLKRAQVETKTDTFYEYHYLDFTTLSNAELRRSAIIKLSSLRDLPLKLQDFYYQGFRLAAIYPFLQPSNKNEKIIQEQIFRAVLIKKMERPLRRESVSEWNSLEIESCFFSDQLPDKSKIPDLIKKIQDAASRGLKFVGIVPQHVFRMSSKSTSAVISISPSSLELKDDKTPLDIPEDCGSLNLEKSNCGDICKVPAEEEECSDQQMNLSEDLGGEQAGTEHINLISSGELARQLQETEILALFNQPATLSTSSQYYTVTIPVRTINDGKSIFQANWLEYMTDHFRKGSTLVHGIFNHGMINDTLQGMTDGVFIFEDLSVDDGSMQGYDAIVVEQWTILDGVHVQTDYIPLLNSLAVYGWQLTCVIPTSIVKTKRDGRLATKQIVFLQRPFLPQKNKRKQSKFHWRFSKQDQHQKQEKKSLKKKLSAGAQQQQREETQESEATNRRSSEAQFPTNDLQLPVISYQQLACAKDRTVEHKPMPSYNSEMSAETGNARQSDDEDITGFCLSQKGAHENCESQMFQEHYTTADDGMFEAEAAETDWIPDVSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT66</accession>
    <name>A0A6I9YT66_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106553215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927154.1">
        <property type="nucleotide sequence ID" value="XM_014071679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT66"/>
    <dbReference type="GeneID" id="106553215"/>
    <dbReference type="KEGG" id="tsr:106553215"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="9">
        <location>
            <begin position="78"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013927154.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="53351" checksum="48F799D45DE5B7BC" modified="2020-10-07" version="1">MGASTHFLSPNNKKRVYGMPKAKCLLALGQGEVDPQIHYKPGDYSISGIVSAMKFKVPIYIQNKQMLVVNSFSLAICFVLGIFIKFRETPIVKANNRDLSYILLISLLFSFLTSFLFIGQPRRVTCLLRQTTFSIIFSVAISTVLAKTFMVVLAFLATKPGNKFQRWLGNSLSNSVILSCSGVQIVLCSIWLGTSPPFPDSDMHSQSGVIILQCNEGSVTMFYIALGYMGFLAAICFTVAFLARNLPAAFNEAKLITFSMLVFCSVWVSFVPTYLSTKGKYMVAVQLFSILASCLHPFLQSSQFHNNSIEGVYLDEKGDLTADLDIVNWVLFPNKSFKRLKSGSLEKLTSKDLKIAIDPKSITQLEMLKKMVLKTISFKVYTHYSMQCSPQCHVLSPMLFNLLTHGYCARFATNNTMKYVDDTKVVGLIQDDSAEIEQSNSPSVITTFYRRTIEIILSYCTTVWFENIIASDRREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTL3</accession>
    <name>A0A6I9YTL3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exocyst complex component 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926990.1">
        <property type="nucleotide sequence ID" value="XM_014071515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTL3"/>
    <dbReference type="GeneID" id="106553084"/>
    <dbReference type="KEGG" id="tsr:106553084"/>
    <dbReference type="OrthoDB" id="27109at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="1">
        <location>
            <begin position="99"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926990.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17619" checksum="6DC2B1E5AF6CDEAC" modified="2020-10-07" version="1">MKSIFFLIYRWLSFQIHEMKIFFSAMLLATHLYTLSKCRSNSELSLMASLASTTVRSRSSHAICLLEKMTAIKHALQRDIFTPNDERLLSIVNVCKAGKKKRNCFLCATVTTERPVQVKVIKVKKSDKGDFYKRQIAWELRDLTVVDAKDAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUD2</accession>
    <name>A0A6I9YUD2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein MON2 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MON2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927646.1">
        <property type="nucleotide sequence ID" value="XM_014072171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUD2"/>
    <dbReference type="GeneID" id="106553636"/>
    <dbReference type="KEGG" id="tsr:106553636"/>
    <dbReference type="CTD" id="23041"/>
    <dbReference type="OrthoDB" id="294853at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199:SF4">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199">
        <property type="entry name" value="NUMOD3 MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HDS" evidence="1">
        <location>
            <begin position="265"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="2">
        <location>
            <begin position="347"/>
            <end position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927646.1"/>
        </source>
    </evidence>
    <sequence length="1132" mass="125616" checksum="217C2E81357BCADC" modified="2020-10-07" version="1">MAALCGKLSLVTSRDAFITAICKGSLPPHYALTVLNTTSAALSSKSYSIQGQNVQMISPSSESHQQVVAVGQPLALQPQGTVMLTSKNIQCMRTLLSLAHCHGAVLGTSWQLVLATLQHLVWILGLKPGIGGALKPGRAVEGPSTVLTTAVMTDLPVISNILSRLFESSQYLDDVSLHHLINALCSLSLEAMDMAYGNNKEPSLFAVAKLLETGLVNMHRIEIMWRPLTGHLLEKVCQHPNSRMREWGAEALTSLIKAGLTFNHDPSLSQNQRLQLLLLNPLKELSNISHPDIRLKQLECVLQILQSQGDSLGPGWPLVLGVMGAIRSDQGESLIRTAFQCLQLVVTDFLPTMPCTCLQIVVDVAGSFGLHNQELNISLTSIGLLWNISDYFFQRSEIIEKELNKEESVLQKQAKENGITLNRPFHPAPPFDCLWLCLYAKLGELCVDPRPAVRKSAGQTLFSTIGAHGTLLQHSTWHTVIWKVLFHLLDRVRESSTTADKEKIESGGGNILIHHSRDTAEKQWAETWVLTLAGVARIFNTRRYLLQSLGDFSEAWDILLDHIQSAALSKNNEVSLAALKSFQEILQIVSPVRETEKPDTPPAINVPVPVLIGSMTATGLGRSFMRTDSLGERIGKCSAIEPPMITDEIEDLDLWWAAWNSWFRIGSESTKPPLSFDKMTFIPSQPFLTALIQIFPALYQHIKTGFSMDDLQKLGVILHGAISVPISSDASPFILPSYTEAVLTSLQEAVLTALDVLQKAICIGSENMQVMYPAIFDQLLAFVEFSCKPPQYGQLETKHIANAKFNQIQLFAPAEWVALNYVPFAERSLEVVVDLYQKTACHKAVVNEKVLQNIIKTLRMPLSLKYACPSESTWKLAVSSLLKVLSIGLPVARQHASSGKFDSMWPELATTFENFLFTKSLPPDNLSIQEFQRNENIDVEVVQLISTEILPYANFIPKEFVGQIMTMLNKGSIHSQSSSFTEAEIDIRMREEFSKMCFETLLQFSFSNKVTTPQEGYISRMALSVLLKRSQDVLYRYIEDERLSGKCPLPRQQVTEIIFVLKAVSTLIDSLKKTQPENVDDNTWAQVIALYPTLVECITCSSSEVCSALKEALVPFKDFMHPPVSKVQNGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUE4</accession>
    <name>A0A6I9YUE4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Death effector domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">DEDD</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927609.1">
        <property type="nucleotide sequence ID" value="XM_014072134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUE4"/>
    <dbReference type="GeneID" id="106553592"/>
    <dbReference type="KEGG" id="tsr:106553592"/>
    <dbReference type="CTD" id="9191"/>
    <dbReference type="OrthoDB" id="6422954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008625">
        <property type="term" value="P:extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038856">
        <property type="entry name" value="DEDD/DEDD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049341">
        <property type="entry name" value="TRADD-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15205">
        <property type="entry name" value="DEATH EFFECTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15205:SF2">
        <property type="entry name" value="DEATH EFFECTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20694">
        <property type="entry name" value="TRADD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRADD-like N-terminal" evidence="2">
        <location>
            <begin position="128"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927609.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25759" checksum="D11763AE089796CE" modified="2020-10-07" version="1">MLLPIRPSHVFLSSPSVCPDLVDKYLEETSIRYVTPRDHSNTEHLPVHQHKSVPPHHPLVCCSSSGPQICAKRSGRGRALLGNQRKRRKSVTPDPKDKQTCDIRLRVRAEYCQHETALEGNVFSNKQDPLERQFERFSQANTILKSRDLGSIICDIKFSELTYLDAFWRDYINGSLLEALKGVFITDSLKQAVGHEAIKLLVNVDEEDYEVGRQKLLRNLMLQTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YW80</accession>
    <name>A0A6I9YW80_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Death-inducer obliterator 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106553810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927855.1">
        <property type="nucleotide sequence ID" value="XM_014072380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YW80"/>
    <dbReference type="GeneID" id="106553810"/>
    <dbReference type="KEGG" id="tsr:106553810"/>
    <dbReference type="OrthoDB" id="1884872at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF13">
        <property type="entry name" value="DEATH-INDUCER OBLITERATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spen paralogue and orthologue SPOC C-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="436"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="662"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1066"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="391"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="664"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="737"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="772"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="837"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="924"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="973"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1012"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1066"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013927855.1"/>
        </source>
    </evidence>
    <sequence length="1189" mass="132722" checksum="F1C615FAD0582F91" modified="2020-10-07" version="1">DLPDTIHIGGRISPKTVWDYIGKLKSSVSKELCLIRFHPATEEEEAAYISLYSYFSSRCRFGVVANNNRHVKDLYLIPLRAKDPIPSKLLPFEGPGLESNRPNLILGLVICQKTKRPATVLEPEKPEEKRSRVQIQEETEVLGYSKGSTLPPPEKKTAKFSLYSGETTISTTPPDSPPPPPPNPPSEPSSTMAPSVLKILSSVKAGSSSTVAPSNASGATTTPVSSASSSASKSSTPLDHILQTLFGKKKSFDPAANEPEATPHTSKPEAQVGSDEGPLAAPLIDPIVQQFGQMSKDKAIEEEEDDRPYDPEEEYAPERAFEMQAGESRLEKGCETLEREDEAYDPEDETILEEAKVAVEDLPNKMYLDSKNSSITTSAPFVPDAAPAPSLVEQQKMLEELNKQIEEQKRQLEEQEEALRQQRAAVGVSMAHFSVSDALMSPPPKPSIAKTELFQHEKPISEKSEMPPSSIQPQLLGQGNDPRQSRDPRQARRILTECNESVDFNIKQHGIQGEPSAGQIPPPSYSGPLQAAFDKEEKCHNETWMASETGPPAVPEQNVPLSYENIPQIHPEIINKPAPIESPTSAPIRKVLLPTPPTPCFMPVFSGSNNAQTVTWSNASQDNSVPGIVRDSFPSSTFVSQEKIPGHFEAERGPLNYNEGEVRPQSSHFYEQRESSTIQTEEVRGVPPFPPAGQKVAPPVLCNASDFHGQRGPPQPYNEGHNVPPNNDGQRGPPPSRFSAPRAPIPSLFSAPHGPPVFVENKGPSPSFPGGPREKAPSQFEDHRESQAEQREFSDSQYHEVSGPSGQFEGSEPLQFVGNRGPPPFSYGGQRRPPPNQFKNQRGGPSTQFGGPRGPSPNHFGGTRGPPPNQFEGQRGPAPGHMPGPRGLLPSPFEERRGSPPPRFPNQRGSASHQFGGPRGQAPASFSEQNDSAPNRHNFPGQSQQGMKPSPRPLLDLPSHPPQHRKEIWDESGPSSSHPNISGQASSSDAQWPASDFREGRNNDYRGQGFDRGPRERFEGGNKEKVLEQPEKSDNRSNRGNDDRRRDREHNWAWERDRGRNWNRGRERDWERHKEKDWDKNRDRNYNKDRERESDRVKDWERNRERPRMRECDSYRRRNRSRSRERDYDRFRDRARSRERERDRDRDRDKDRGRDRKERSKSTECDRDSKPESQMGTQKPSHPEAASAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWI9</accession>
    <name>A0A6I9YWI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Contactin-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Brain-derived immunoglobulin superfamily protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Plasmacytoma-associated neuronal glycoprotein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC106554500</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with PTPRG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. Contactin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928662.1">
        <property type="nucleotide sequence ID" value="XM_014073187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWI9"/>
    <dbReference type="GeneID" id="106554500"/>
    <dbReference type="KEGG" id="tsr:106554500"/>
    <dbReference type="OrthoDB" id="5982258at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000035">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000044">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000047">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000052">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000054">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000064">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000273">
        <property type="entry name" value="contactin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF18">
        <property type="entry name" value="CONTACTIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026841726" description="Contactin-3" evidence="19">
        <location>
            <begin position="19"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="26"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="122"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="227"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="318"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="408"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="499"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="601"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="704"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="806"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="687"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="689"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013928662.1"/>
        </source>
    </evidence>
    <sequence length="977" mass="108364" checksum="AFA5F7F24109D218" modified="2020-10-07" version="1">MRLSWELLVLQSFMLCLADDNILHGPVFIQEPSSVIFALDSEEKKVKLNCEVKGNPKPTIRWKLNGTIVDIGMDYRYSVVEGSLLINNPNKTQDAGTYQCIATNSFGTIVSREAKLQFAYLENFKTRTRSTVSVRQGQGMVLLCGPPPHSGELIYAWIFNEYPTFVHQDNRRFVSQETGNLYIAKVEKSDVGNYTCVVTNTVTNTRVRGPPTPLILRNDGVMGEYEPKIEVQFPETVPSAKGTTVRLECFALGNPVPTISWRRTDGKQIPKKARRHKSSSILEIPNFQQEDAGMYECVAENVRGKNVARGQLTFYAPPNWNQKISDIQVAIEESIYWECKANGRPKPSYNWLKDGELLLPQERLHIEHGTLTIPSVNLSDTGMYQCVAENRYGVIYVSAELSVIAVGPDFSKTFLKKITFVKVGSDATIECKPKASPRPTFTWKKGKEIIKESERHTLLEDGTLKIANVTKADAGSYTCIATNHFGVASSTGNLIVKDPTRFVVAPTSMDVTAGESILLPCQVSHDHPLDIVFTWSFNGRLIDFEKDGDHFERVGGQDSAGDLMIRSIQLNHAGKYVCMVQTSVDKLSAAADLIVRGPPGPPEAVTIDEITDTTAQVSWRPGADNRSPITTYVIQARTPFSVGWQGVNTVPDVIDGNTFTATVVGLNPWVEYEFRIVAASLIGIGEPSKPSEKRRTEEALPEVTPANVSGGGGTKSELVITWETVPEELQNGGGFGYVVAFRPVGTISWMQTVVVSPDASKYVFRNESLPPFSPYEVKVGVYNNKGEGPFSFITVVYSADEEPTRAPTNVFAKSLSASDIEVFWSLLPETQSKGRIQGYEVRYWKHGDKEEQAGRMKTMTNQTFAKITNLKSSALYHLAVKAYNTAGMGPSSAIVNVTTKKPPPSQPPGNIIWNSSDSKIILNWDQVKAQNNESEVKGYKVFYRWNRQNSMSIIETNKTSVELSLPFDEDYIIKICI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWP9</accession>
    <name>A0A6I9YWP9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">D(4) dopamine receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DRD4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928035.1">
        <property type="nucleotide sequence ID" value="XM_014072560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWP9"/>
    <dbReference type="GeneID" id="106553964"/>
    <dbReference type="KEGG" id="tsr:106553964"/>
    <dbReference type="CTD" id="1815"/>
    <dbReference type="OrthoDB" id="10010417at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001591">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gi/Go"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007195">
        <property type="term" value="P:adenylate cyclase-inhibiting dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051481">
        <property type="term" value="P:negative regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051967">
        <property type="term" value="P:negative regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060158">
        <property type="term" value="P:phospholipase C-activating dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014059">
        <property type="term" value="P:regulation of dopamine secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043266">
        <property type="term" value="P:regulation of potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002185">
        <property type="entry name" value="Dopamine_D4_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF143">
        <property type="entry name" value="D(4) DOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00569">
        <property type="entry name" value="DOPAMINED4R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928035.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="33841" checksum="4DC884923F03D41F" modified="2020-10-07" version="1">MHSLIFLQFQGGVWSLGPVLCDALMTMDVMLCTASIFNLCAISVDRFIAVSVPLNYNRRQIDLRQLILLSTTWIFAFAVASPIIFGLNNVPNRDPSFCRLEDHNYIVYSSICSFFIPCPVMLVLYCGIFHGLRRWEEARKAKLKSSIYLGNRQVYGPPPVIEKSQEDIKAEELNPCACSDCTLPRDYKNNGIQTISYPHIKYAPNGELSRKPAKINGRERKAMKVLPVVVGAFLFCWTPFFVVHITRALCESCIRSGQVISIVTWLGYVNSALNPIIYTIFNTEFRNYFRKVLHIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX83</accession>
    <name>A0A6I9YX83_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">UGDH</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013928644.1">
        <property type="nucleotide sequence ID" value="XM_014073169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YX83"/>
    <dbReference type="GeneID" id="106554488"/>
    <dbReference type="KEGG" id="tsr:106554488"/>
    <dbReference type="CTD" id="7358"/>
    <dbReference type="OrthoDB" id="5059218at2759"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000114">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028356">
        <property type="entry name" value="UDPglc_DH_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374:SF59">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374">
        <property type="entry name" value="UDP-GLUCOSE DEHYDROGENASE/UDP-MANNAC DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="4">
        <location>
            <begin position="76"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013928644.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="27425" checksum="62A7D52724ECB2EF" modified="2020-10-07" version="1">MDQRIGNKFLKASIGFGGSCFQKDVLNLVYLCEALNLSEVARYWQQVIDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGARLHIYDPKVLKEQIILDLSHPGVSEDDQVSRLVTISKDPYEACEEAHALVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDDLHHRLQAIGFQIETIGKKISAKRIPFASSGDIPKFSLQDPPLKKPRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXF6</accession>
    <name>A0A6I9YXF6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">BMP and activin membrane-bound inhibitor homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BAMBI</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 13">Negatively regulates TGF-beta signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 13">Belongs to the BAMBI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928982.1">
        <property type="nucleotide sequence ID" value="XM_014073507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXF6"/>
    <dbReference type="GeneID" id="106554772"/>
    <dbReference type="KEGG" id="tsr:106554772"/>
    <dbReference type="CTD" id="25805"/>
    <dbReference type="OrthoDB" id="5914644at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23576">
        <property type="entry name" value="TFP_LU_ECD_BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000018">
        <property type="entry name" value="BMP and activin membrane-bound inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009345">
        <property type="entry name" value="BAMBI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045806">
        <property type="entry name" value="BAMBI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045807">
        <property type="entry name" value="BAMBI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15505:SF1">
        <property type="entry name" value="BMP AND ACTIVIN MEMBRANE-BOUND INHIBITOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15505">
        <property type="entry name" value="RIIA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06211">
        <property type="entry name" value="BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19337">
        <property type="entry name" value="BAMBI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037456">
        <property type="entry name" value="BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026655285" description="BMP and activin membrane-bound inhibitor homolog" evidence="15">
        <location>
            <begin position="27"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor N-terminal" evidence="16">
        <location>
            <begin position="4"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor C-terminal" evidence="17">
        <location>
            <begin position="123"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF06211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF19337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013928982.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="29330" checksum="8B78B13DAC2E1433" modified="2020-10-07" version="1">MERHSSFFFIWLQLELCAMAILLTKGEIRCYCDAAHCVATGYMCKSELSACFSRLLDPQNSHSPLTHGCLDSLASTLDICQVNIAQNHSGIAIPTLECCHEDMCNYRGLYDVLSTSRGDPLGQGSRYQHDNSRNLITKVQELTSSKELWFRAAVIAVPIAGGLILVLLIMLALRMLRSENKQLQDQRQQMLSRLHYSFHGHHSKKGQMAKLDLECMVPVTGLENCCMTCEKMRQPDYSNDRILSLVHWGMYGGHGKLEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXY1</accession>
    <name>A0A6I9YXY1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein LOC106554326</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106554326</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928450.1">
        <property type="nucleotide sequence ID" value="XM_014072975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXY1"/>
    <dbReference type="GeneID" id="106554326"/>
    <dbReference type="KEGG" id="tsr:106554326"/>
    <dbReference type="OrthoDB" id="6376697at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005916">
        <property type="term" value="C:fascia adherens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021623">
        <property type="entry name" value="LAP2alpha_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914">
        <property type="entry name" value="ALPHA CATENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914:SF21">
        <property type="entry name" value="CATENIN ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11560">
        <property type="entry name" value="LAP2alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lamina-associated polypeptide 2 alpha C-terminal" evidence="5">
        <location>
            <begin position="248"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="371"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928450.1"/>
        </source>
    </evidence>
    <sequence length="441" mass="48923" checksum="A7455010FC05C087" modified="2020-10-07" version="1">MEDFSSHIVNSEANLAETIFAACHKDLKSNSHRDEIAAARASLKESSPLLHSVCSACLEHSDVASLTASKDSVCQEIQNALDVISKASQGIEHPKVQQVPQSASLGSALDELASLVALDSITVSEEQIRPSLEKRLEGIISGAALLADSSCTRDLHRERIITECNAIRQALQDLLSEYMNNANATADLDPPTDPKPVSETPSTSKGKKKGPFHRPPVAKEEIPTPEMFLEVLKSQWVPEVDKPIATLALVSAVITGDIASCLKAENNKVDGAVVGAAEGQASEVRLLQDISKILAATQFSADTTLDAVKYVSWTMSSSMASRRLLWLHHWQAESRMKWHLTGADYTGDKLFGTALDPILVEDKNKRKVFPATTRRSDFQHAPYPRRSSYRSSDVETQNQRYQNPRSHREGDRQFHRDRFRDQQQGKWPFREGGSRPFRRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZN4</accession>
    <name>A0A6I9YZN4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CDK5 regulatory subunit-associated protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106554859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929070.1">
        <property type="nucleotide sequence ID" value="XM_014073595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZN4"/>
    <dbReference type="GeneID" id="106554859"/>
    <dbReference type="KEGG" id="tsr:106554859"/>
    <dbReference type="OrthoDB" id="10255000at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090063">
        <property type="term" value="P:positive regulation of microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903358">
        <property type="term" value="P:regulation of Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052593">
        <property type="entry name" value="MT-associated_AKAP9-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46501">
        <property type="entry name" value="MYOMEGALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46501:SF7">
        <property type="entry name" value="MYOMEGALIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013929070.1"/>
        </source>
    </evidence>
    <sequence length="185" mass="21128" checksum="1B0142B8B31E4C72" modified="2020-10-07" version="1">LSFSTLDSPSHCSLLTNQEICPIPSHCVWADKNGQHILGLIEDYNCLRKQITEGRKRLSKLELPLREEGDPELAVTVPLSLSATFKAVQENLEEAGRLLTLLWRVSLPMKVVHAAAYSLQDEDLKSEVYKLRRKVAEQEKKLFSMARRLYSTNQLKENMERVIMEQSSNIKIGVRGKHFLKVPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z299</accession>
    <name>A0A6I9Z299_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Selenoprotein W</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SEPW1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role as a glutathione (GSH)-dependent antioxidant. May be involved in a redox-related process. May play a role in the myopathies of selenium deficiency.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SelWTH family. Selenoprotein W subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930354.1">
        <property type="nucleotide sequence ID" value="XM_014074879.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555938"/>
    <dbReference type="KEGG" id="tsr:106555938"/>
    <dbReference type="CTD" id="6415"/>
    <dbReference type="OrthoDB" id="444492at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000158">
        <property type="entry name" value="Selenoprotein W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051441">
        <property type="entry name" value="SelW_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15124">
        <property type="entry name" value="SELENOPROTEIN W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15124:SF18">
        <property type="entry name" value="SELENOPROTEIN W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0711">Selenium</keyword>
    <keyword evidence="4 10" id="KW-0712">Selenocysteine</keyword>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="10">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013930354.1"/>
        </source>
    </evidence>
    <sequence length="85" mass="9372" checksum="B3DB2F8344DF1807" modified="2020-10-07" version="1">MAVKIKVVYCGAUGYKPKFLQLKKELEKEFLGKLEISGEGTPQVTGWFEVIVAGKTVHSKKNGDGFVDDDVKLNKIIKAIKANLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2E7</accession>
    <name>A0A6I9Z2E7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Huntingtin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106555802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930191.1">
        <property type="nucleotide sequence ID" value="XM_014074716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2E7"/>
    <dbReference type="GeneID" id="106555802"/>
    <dbReference type="KEGG" id="tsr:106555802"/>
    <dbReference type="OrthoDB" id="10065698at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048412">
        <property type="entry name" value="Htt_bridge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048413">
        <property type="entry name" value="Htt_C-HEAT_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028426">
        <property type="entry name" value="Huntingtin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10170:SF10">
        <property type="entry name" value="HUNTINGTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10170">
        <property type="entry name" value="HUNTINGTON DISEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20925">
        <property type="entry name" value="Htt_bridge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20927">
        <property type="entry name" value="Htt_C-HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930191.1"/>
        </source>
    </evidence>
    <sequence length="934" mass="104579" checksum="DCADA8EF04E4BE7D" modified="2020-10-07" version="1">CPVGPSARVNPFHFFPQCQNLHDSEHLTWLTVNHVQELISLSHEPPVQDFISAIHRNSAASGLFIQAIQSRCENFASPTAVKKTLQCLEGIHLSQSGAVLVLYVDKLLSTPFRVLAHMVDTLACRRVEMLLAASVQNSTAQLPVEELERIQEYLQNTGLADRHQRLYSLLDRFRLTVAPETISPSPLVTAHPLDGGKHPALETLIPDKDWFCSLVRSQCYTKSAFALPEGAELMNRLPEEELIPFMMHQDFNVSLLAPCLSLGGQELSRGLRSHFEAARLVTLHQVTEVVHRLPAGHQVFQPTQPVGSSPYWNKLSNIYGDPEMFQFTMVLAQALSQYLVLLSKLPTSLRIPPEKENDIAKFVVMLLEILSWHLARKQVPLSMDFQAVLDCCCLALQQTELWNLWASAENTSQASSLIHCTRFILEAVAIEPGNRLLSPEKRKKIQKAGSENEKASQTPNSESITATCEVVAELVECLQSVLALGHKKNSPIPAFLTPVLRSIIISLSRLPLVNSYARVPLLVWKLGWSPRPTGEFGTTFPEIPVEFLQEREIFKEFIYRINTLGWTNRMQFEETWASLLGVLVTQPIAMDQEENQQEEDMERTQINVLAVQAITSLVLSAMTIPLAGNPAVSCLEQQPRNKTLKALDTRFGRKLNIIRGIVEQEIQEMASKRDNVACHHVYQVWDPVPSLAPSTTGSLISHEKLLLQINTEREMGNMSYKLGQVSIHSVWLGNSITPLREEEWGEDEDDESDMPALSSPPSSPINSRKHRAGVDIHSCSQFLLELYSQWILPSTPNKKTPVTLISEVVRSLLAVSDLFTERNQFEMMYATLTELRKVHPSEDEILLQYLVPAVCKAAAVLGMDKAVAEPVSRLLESTLRSAHMPSRIGALHGILYILECDLLDETAKQLIPVITEYLLSNLRGVAHCVHLHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z377</accession>
    <name>A0A6I9Z377_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Insulin gene enhancer protein ISL-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ISL1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930679.1">
        <property type="nucleotide sequence ID" value="XM_014075204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z377"/>
    <dbReference type="GeneID" id="106556214"/>
    <dbReference type="KEGG" id="tsr:106556214"/>
    <dbReference type="CTD" id="3670"/>
    <dbReference type="OrthoDB" id="125004at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048665">
        <property type="term" value="P:neuron fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09374">
        <property type="entry name" value="LIM2_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000041">
        <property type="entry name" value="insulin gene enhancer protein ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000056">
        <property type="entry name" value="insulin gene enhancer protein ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047169">
        <property type="entry name" value="ISL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204:SF4">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 14 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 14 16" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 15" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="19">
        <location>
            <begin position="111"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="14">
        <location>
            <begin position="113"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="244"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013930679.1"/>
        </source>
    </evidence>
    <sequence length="281" mass="31232" checksum="07CEC639813BDFA4" modified="2020-10-07" version="1">GSEPRLYGIKCAKCNIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQVNPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3E6</accession>
    <name>A0A6I9Z3E6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Beta-secretase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">BACE2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930496.1">
        <property type="nucleotide sequence ID" value="XM_014075021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3E6"/>
    <dbReference type="GeneID" id="106556057"/>
    <dbReference type="KEGG" id="tsr:106556057"/>
    <dbReference type="CTD" id="25825"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050435">
        <property type="term" value="P:amyloid-beta metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05473">
        <property type="entry name" value="beta_secretase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000003">
        <property type="entry name" value="Beta-secretase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000007">
        <property type="entry name" value="Beta-secretase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009119">
        <property type="entry name" value="BACE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009121">
        <property type="entry name" value="BACE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033874">
        <property type="entry name" value="Memapsin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF40">
        <property type="entry name" value="BETA-SECRETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01817">
        <property type="entry name" value="BACE2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01815">
        <property type="entry name" value="BACEFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026698066" evidence="17">
        <location>
            <begin position="23"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="469"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="18">
        <location>
            <begin position="94"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="14">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="14">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="235"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="294"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="346"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR609121-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR609121-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013930496.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="56586" checksum="84DD67A58F10E185" modified="2020-10-07" version="1">MSRTAACVVLLLLLAVAERGRASFAFRLQVSSPVTPESNLVPTPVLLKSRSNGGAGSQPALPEASGLALASDPGGSLNFLAMVDNLQGDSGRGYYFELLLGTPPQKLNILVDTGSSNFAVSGAPNTDLTSYFDTERSSTYKSLNVEVTVKYTQGSWIGVLGTDVIRMPKGINGTYIINIATIFQSENFFLKGIQWQGILGLAYSTLAKPSSSVETFFDSIVNQAKIPNVFSLQMCGPSVPVSGTGTNGGSLVLGGIEPSLYKGKIWYTPIKKEWYYQVEILKFEVGGQNLNLDCKEYNSDKAIVDSGTTLLRLPEKVFSAVVEAITQTSLIQEFTPGFWSGTQLACWDKSENPWALFPKISIYLRDVNSSQSFRITILPQLYIQPILGIGQNLDCYRFGISSSSSALVIGATVMEGFYVIFDRAQKRVGFALSTCAELHGSPVSEIEGPFTTTDVAGTCVSTISLHEPILWIVSYTLMSFCGLVLLILIILLLIPSRCRHRYADCDTVNDESSLVRHRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4F4</accession>
    <name>A0A6I9Z4F4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GPI mannosyltransferase 2</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14 15" type="primary">PIGV</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PIGV family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013931983.1">
        <property type="nucleotide sequence ID" value="XM_014076508.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931984.1">
        <property type="nucleotide sequence ID" value="XM_014076509.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931985.1">
        <property type="nucleotide sequence ID" value="XM_014076510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4F4"/>
    <dbReference type="GeneID" id="106557296"/>
    <dbReference type="KEGG" id="tsr:106557296"/>
    <dbReference type="CTD" id="55650"/>
    <dbReference type="OrthoDB" id="10252502at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007315">
        <property type="entry name" value="PIG-V/Gpi18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468:SF2">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04188">
        <property type="entry name" value="Mannosyl_trans2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013931983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013931984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013931985.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="59773" checksum="56A2B064D3445EC3" modified="2020-10-07" version="1">MDVYNDPSFKARMPLSNCRNTRYQEVVCFAVFCRIVTLLLQALFNFLIPDHAADAFSPPRLSDPGFLDQLLEWFLGGLSRWDAEHFLFIAEHGYVYEHNCAFFPLFPLALKAVASLIFWPLQGFLSFRSCLLLSAVLLNAVFSVLASGTLYELSLAVLQCRRRAFLSAVLFCLTPANVFMAAAYSESLFAFLVFAALWRLEKGHPWLSLLLFSLATGVRSNGLINAGFLIYSQTKLFAFSFQASESVLQKKGQFLKLAAALVLMSASVALPFALFQCYAYLKFCRPNIGLGYAIPKPLLQLAESKGYRVAASEGGMPPWCSWNFPVLYTYIQDTYWNVGFLRYFELKQIPNFLLASPAIVLGSWASWWYVTANPWHCLTLGLVRRKSAGMQDHDSHKPAEGFGSPAVFVYVVHAAALLVFGTFFMHVQVFTRFLGSSSPVLYWFCAHLLYDTEPLLQDGASPSKHRKSFAEKLSVDNFFSTCCRHENPVWMLLSRWKQSRILTKCILGYVLSYWLLGLVLHCNFFPWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5U0</accession>
    <name>A0A6I9Z5U0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Programmed cell death protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PDCD2L</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013932032.1">
        <property type="nucleotide sequence ID" value="XM_014076557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5U0"/>
    <dbReference type="GeneID" id="106557340"/>
    <dbReference type="KEGG" id="tsr:106557340"/>
    <dbReference type="CTD" id="84306"/>
    <dbReference type="OrthoDB" id="366284at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052815">
        <property type="entry name" value="PDCD2-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46421">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46421:SF1">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 2 C-terminal" evidence="1">
        <location>
            <begin position="255"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013932032.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="41662" checksum="CC8D487E022C696A" modified="2020-10-07" version="1">MAAAPARVLLGLRDAAVPPGSEGLPSQGTASKLGGSPDWRLSVPVIHLSCEVCCAGLLHVVQIYCPLQGSQYHRVINVFACARKSCWGNSESWKVLRSQYLQIPRKEIQDCTQKQEQERTQAVTDWCNGADDWGENGEEAPSELSATFHSFPRETQCAALLQRMSLGKNTGDFPDMTQEEPIKPSSCAQEFQPYYISVVEEEDYLCWEDLEDAQRLLKEYQQREDVDLEHLMSASYAPDGSGEKYEKSEAEKRNQIFYKFMKRISSCQEQILRYSWNGQPLFITQPSADFQTRVPRCNNCKSKRIFEFQLMPTLVNMLKTRENDTSIEFGTAVVYTCEKSCWTAGCSSPLEEFIFVQEDPDQRLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z774</accession>
    <name>A0A6I9Z774_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit 15B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PPP1R15B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP1R15 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931741.1">
        <property type="nucleotide sequence ID" value="XM_014076266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z774"/>
    <dbReference type="GeneID" id="106557117"/>
    <dbReference type="KEGG" id="tsr:106557117"/>
    <dbReference type="CTD" id="84919"/>
    <dbReference type="OrthoDB" id="5976067at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051254">
        <property type="entry name" value="PPP1R15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019523">
        <property type="entry name" value="Prot_Pase1_reg-su15A/B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16489">
        <property type="entry name" value="GH11727P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16489:SF11">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 15B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10488">
        <property type="entry name" value="PP1c_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 15A/B C-terminal" evidence="3">
        <location>
            <begin position="257"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931741.1"/>
        </source>
    </evidence>
    <sequence length="481" mass="54066" checksum="212939F889E7838B" modified="2020-10-07" version="1">MEPLREGEQAASRLNACPPSLPASQPDAPFSLLRLLAQLLQRLLPGPISPLFWLPSDVHATAPETLVPVWETPGLSPILAVQHQLRVVSSSYVLGGTDKNNSVAGSLRAKFPELLQPAPWQPHKAVLDEPETDCGYHSLEVEEQLREAWAPKDRGGEAQEERDPAKASQSLDSDGAEREAAAEVRSEIEAHLPLVARPACTNKLIDYILGGACSGDENEEDSNDEDEEEEEDWYEEEEDDDDGFDSEGSLSESEISQDGESSDLWNSFCTPDPYDPWNFTAAMQTAENMGEEGWKRESNEGEVVEESSSWSESSCEEDEWESGSLDESENLKLWNSFINLDDPYNPFHFKAPFQTAGKKGKSDLKGSPGLSLSPSQHSILLSCQLRLFDNGSTENVRYGILSKEKCKKKKKVTFLEEVTEYYVSIEEDRKGPWEELARDGCRFHKRIQETEDAIGYCLTVEHRQRIRNRLQEMCYERPDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WYZ4</accession>
    <name>A0A6I9WYZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Olfactory receptor 13G1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106537689</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907356.1">
        <property type="nucleotide sequence ID" value="XM_014051881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WYZ4"/>
    <dbReference type="GeneID" id="106537689"/>
    <dbReference type="KEGG" id="tsr:106537689"/>
    <dbReference type="OrthoDB" id="6145535at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15232">
        <property type="entry name" value="7tmA_OR13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013907356.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="34387" checksum="14E269054C42D866" modified="2020-10-07" version="1">MRNKSCVTTFVMIGLSEKPKLQLMLFVLFLVVYIIALIGNLAIIAAIISYPKLHRPMYFFLFHLAVVDIICTSAIIPKMLGNIATSSKGISYSGCIVQLYSFTWCLGAEMVLFTIMAYDRYVAICHPLHYTIMMSKKVCMALSTVVILIAISNSWVHTGLIMRLTFCGPNGINHFFCEIPPLLALSCSPVRLNEVWVFTADIFLAMGNFMLTCLSYCFIVRTILKIKTSEGKKKAFSTCSSHLIVVTLYYSTVIYTYIRPASSYNFQRDKAVAALYTLVTPVLNPIVYSLRNNDVQAALKKVFFIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X095</accession>
    <name>A0A6I9X095_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">EF-hand domain-containing family member B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">EFHB</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907187.1">
        <property type="nucleotide sequence ID" value="XM_014051712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X095"/>
    <dbReference type="GeneID" id="106537543"/>
    <dbReference type="KEGG" id="tsr:106537543"/>
    <dbReference type="CTD" id="151651"/>
    <dbReference type="OrthoDB" id="2096280at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057428">
        <property type="entry name" value="EFHB_EF-hand_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF12">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25325">
        <property type="entry name" value="EF-hand_EFHB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="302"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="338"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013907187.1"/>
        </source>
    </evidence>
    <sequence length="571" mass="65300" checksum="AE553DD782F84E71" modified="2020-10-07" version="1">MVGGLPVKRLRGTVGQVYQGKYIDRFPELPTAGKLIPIGETAASCLTEILPKPITPPVVQKFRNTSNPPTGKERVFYGRANDPDIASYLTHGITTVLPENASPLINPPPKTCFQQKIKEKKESVYLSSCQAPLGKSHDQSLRLPKGFDVNNTRFGTKVKKEISAADIVNPQKTFDEVQKEAQEGHDLYVTTHNDYFVGEAVNRKYNPSTFNRFNLYGKETPHFNDGRNIGRTLHWLHDLQMYIADTLNVPPDHTFGILIQPEEYGVGDLLHNRLPSEYLKSKDRERAVFTALRQNLKKANYHNFDTLLHAFRHYDKDGDRKISREDLKKACFQLNLDLDDELLDSLFNYCDLDKDGFINYLEFVNFLNWKDKMAIQEFEEKIITKGKKTAPFDLIPAYDSEIKANENALLKQDDIVHMEPGSLEKTARTLSRPTDRVFSDYRTTSSQYNAIVGGIPTSFYPLYGIPTIRSDIPAPRFRRISDTTNYGDQATMYALLYPSIHNKKGVYEKDFFRIRSKEEISDILRNIGVKLSDESFDEVWRQACLKDHRGKVCVESIRNVLDEMQALHLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0C9</accession>
    <name>A0A6I9X0C9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin-protein ligase RNF130</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Goliath homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">RING finger protein 130</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">RNF130</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013908694.1">
        <property type="nucleotide sequence ID" value="XM_014053219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0C9"/>
    <dbReference type="GeneID" id="106538653"/>
    <dbReference type="KEGG" id="tsr:106538653"/>
    <dbReference type="CTD" id="55819"/>
    <dbReference type="OrthoDB" id="5357315at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02122">
        <property type="entry name" value="PA_GRAIL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16803">
        <property type="entry name" value="RING-H2_RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000011">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF21">
        <property type="entry name" value="RING FINGER PROTEIN 130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 23" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026987640" description="E3 ubiquitin-protein ligase RNF130" evidence="23">
        <location>
            <begin position="32"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="268"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013908694.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="47005" checksum="2E92E89A55BD3118" modified="2020-10-07" version="1">MILNWILPYWSYLSSVPILVLFNCYPLQASADNSNLIYYTALINVTVLNPDRSTSASITLERARYGQTSPKIGVKGLLLAPLPINGVPDRLGCDPRTRFQVPPNTKQWIALLQRGNCTFKQKILRAASHNASAVVIYNNVSGNEPVTMTHQGTGDIVTVMITEMKVKEILNYLEKNMSVLIAIAVGTRHPSKNVNRSSLVFVSVSFIVLMIISSAWLIFYFIQKIRYTSARDRNQRRLGDAAKKAVGKLTARTVKKGDKETDPDFDHCAVCIESYKQNDVVRILPCKHVFHKTCVDPWLSEHCTCPMCKLNILKALGIVTNVPCADNMAFDMERLTRGQLGNRGSVPGDLSSDSSLTLEPLRISGISQFPQDGELTPRTGEINIAVTKEWFIIASFGLLSALTLCYMIIKATASLNANEAEWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2A3</accession>
    <name>A0A6I9X2A3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106538492</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908461.1">
        <property type="nucleotide sequence ID" value="XM_014052986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2A3"/>
    <dbReference type="GeneID" id="106538492"/>
    <dbReference type="KEGG" id="tsr:106538492"/>
    <dbReference type="OrthoDB" id="6692864at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008397">
        <property type="term" value="F:sterol 12-alpha-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000025">
        <property type="entry name" value="Prostaglandin I2 (prostacyclin) synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024204">
        <property type="entry name" value="Cyt_P450_CYP7A1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24306">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24306:SF0">
        <property type="entry name" value="7-ALPHA-HYDROXYCHOLEST-4-EN-3-ONE 12-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000047">
        <property type="entry name" value="Cytochrome_CYPVIIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="15">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013908461.1"/>
        </source>
    </evidence>
    <sequence length="509" mass="59049" checksum="7F2C185D4DE7E8C6" modified="2020-10-07" version="1">MTFWVAVAVALAVGLLGLLYLAGSFRQRRANEPPLDKGLIPWLGHAVYFRNDNMEFLKTMQKKHGDIFTTLIAGNYFTFLIDPFSFGAIVKEARSKLDFISFAAELVLRVFRFEANHESHKIIEVSSAKHLKGEGLVVMTQSMMDALQTVLFQWQGTSTEDPKSWKEDGLFHFCYNKVFRAGYLALFGIEPSDKQKEKSKKEDEQHTEDVFVEFRKYDKLFPRLSYALLTPPEWGQVKKIWNYFWQMLSVKNVYKKDNISRWVSDQSQQLAESGVSEEMRDRFMFLLLWAAQGNTGPASFWLLVNLMKHPKAMEEVKKEIEEVVKKSGQDPASRQKPFNVTKEMLSQTPILDSAVEETLRLATAPLLVRLILEDMDLKLHNGKTYKLRKGDKICLFPYLSSHMDPEIHQDPQAFKYDRFLSQNGNKKEFFKNGEKVKYYAMPWGAGTSMCPGRYFATNEIKLFAFLMLACYDLELINQQEKIPAINKTRYGFGVLQPMNDIQFRYRCRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2A5</accession>
    <name>A0A6I9X2A5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Troponin T, cardiac muscle isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TNNT2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909404.1">
        <property type="nucleotide sequence ID" value="XM_014053929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2A5"/>
    <dbReference type="GeneID" id="106539198"/>
    <dbReference type="KEGG" id="tsr:106539198"/>
    <dbReference type="CTD" id="7139"/>
    <dbReference type="OrthoDB" id="330499at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030172">
        <property type="term" value="F:troponin C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031013">
        <property type="term" value="F:troponin I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000001">
        <property type="entry name" value="Troponin T, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF5">
        <property type="entry name" value="TROPONIN T, CARDIAC MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="129"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="129"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013909404.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="35075" checksum="95214BE64FD84A09" modified="2020-10-07" version="1">MSDTEEVIEEYEEEQEEDCIEQEEEEWIEVDDEDDDEQVEEIKEDAEEAIAEEPEEQAKEQEEGESKPKPKLFTPNLIPPKIPDGERVDFDDIHRKRMEKDLNELQGLIEAHFENRKKEEEELIFLKDRIEQRRAERAEQQRIRSEREKERQARLAEERARKEEEEARKRTEEDERKKKAFSNMLHFGGYLQKTEKKSGKKQTEREKKKKILTERRKPLNIDHLNEDKLREKAKELWQSIHDLEAEKFDLQEKFKRQKYEINVLRNRISDHQKVKGSKAARGKPVVGGRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2S3</accession>
    <name>A0A6I9X2S3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="4 10">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NADSYN1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="4 10"/>
    <dbReference type="RefSeq" id="XP_013908137.1">
        <property type="nucleotide sequence ID" value="XM_014052662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2S3"/>
    <dbReference type="GeneID" id="106538221"/>
    <dbReference type="KEGG" id="tsr:106538221"/>
    <dbReference type="CTD" id="55191"/>
    <dbReference type="OrthoDB" id="2020662at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000007">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 10" id="KW-0520">NAD</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="11">
        <location>
            <begin position="5"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013908137.1"/>
        </source>
    </evidence>
    <sequence length="697" mass="78530" checksum="6C4113898AE3B2B4" modified="2020-10-07" version="1">MGRTVTVATCALNQWALDFEGNLERILKSIEIAKEKGARYRLGPELEICGYGCSDHYYESDILLHSFQVLAKLLESPGIQDIICDIGMPIMHRNVRYNCRVIFLNKKILLIRPKIALANSGNYREMRWFTPWNRLRHVEEYFLPRMIQEITAQESVPFGDAVLATLDTCIGTEICEELWIPTSPHIEMGIDGVEIFTNSSASHHVLRKADTRVELVNSATAKNGGIYVLANQKGCDGDRLYYDGCAMISMNGATVAQGRQFSLDDIEVLVATLDLEDVRSYRAEISSRNLAASKVTPYPRIKVNFALSNSDDTCTPTNEPIQWKYHSPEEEISLGPACWLWDYLRCSKQGGFFLPLSGGVDSSSAACIVYSMCLQICYAVKNGNQTVLDDVRRIVNDEMYVPKEAQELCGRLLTTCYMASENSSQDTYDRAKSLAKEIGSYHININIDGAVKAILGIFSTVMRRSPQYQVHGGSTRENLALQNLQARIRMVIGYLFAQLCLWTRGMPGGLLVLGTANVDESLRGYFTKYDCSSADINPIGGISKTDLKCFMQYCIENFQLTSLKSIISAPPTAELEPLQKGNVTQMDEVDMGMTYAELSVYGKLRKIAKAGPYTMFCKLVTMWKEICSPREVASKVKHFFQMYSINRHKMTTLTPSYHAANYSPDDNRFDLRPFLYNSAWSWQFRCIDEQVSKLEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5C3</accession>
    <name>A0A6I9X5C3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tyrosyl-DNA phosphodiesterase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TDP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910599.1">
        <property type="nucleotide sequence ID" value="XM_014055124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5C3"/>
    <dbReference type="GeneID" id="106540111"/>
    <dbReference type="KEGG" id="tsr:106540111"/>
    <dbReference type="CTD" id="55775"/>
    <dbReference type="OrthoDB" id="47785at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09193">
        <property type="entry name" value="PLDc_mTdp1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000012">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910599.1"/>
        </source>
    </evidence>
    <sequence length="455" mass="52004" checksum="2FF508A0A475E393" modified="2020-10-07" version="1">MSQESQHGKWTLSSSEDSMDELADSEKPSTSVTQRGHESKKSSHSVSEARKAANKRKMSPLKYVAGNFPKEEEASDQKFAREDFGWCLSSSEDEDLKTSSRNKDAITTVRKAESGTLMELPTACKIPSSHKSEEPSEVQDTWDLLNGQDPFRFFLTKVKGIKSKYNSRALHIKDILSPLFGTLVSSAQFNYCIDVEWLVQQYPKEFRDKPLLIVHGEKRESKAELHEDAHPYKNVRLCQAKLDIAFGTHHTKMMLLNYEEGLRVVIHTSNLIDDDWYQKTQGIWLSPLYPRLPPGSPESDGESHTHFKSDLISYLISYKSASLMEWVEIIKQHDLSETRVYLIGSTPGRYQGDAKEKWGHLKLRKLLKEHATQIPDQDSWSIIGQFSSIGSMGVDQSKWLCSEFRESLGSLGKKLKTPPNEIPIHLIYPSVENVRRSLEGYPVFYFFFSWRFSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X631</accession>
    <name>A0A6I9X631_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 397-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106540353</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910904.1">
        <property type="nucleotide sequence ID" value="XM_014055429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X631"/>
    <dbReference type="GeneID" id="106540353"/>
    <dbReference type="KEGG" id="tsr:106540353"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001005">
        <property type="entry name" value="Zinc finger protein 75A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF423">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="15">
        <location>
            <begin position="195"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="558"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013910904.1"/>
        </source>
    </evidence>
    <sequence length="582" mass="65660" checksum="435FC25348177705" modified="2020-10-07" version="1">MAATQEVPPPALGLHFLYPLEETMAPRVKIEEPGPPCPEAGWEWDAPGKTPLVVQAGTISDLLRWATPPQVRSDPIPQLWEVQCQEMVQSLRTPADVMPAPVPVPVPPPAPGWSNLQLPELAPVDDIEAYLSSFERAAESCQWPRGEWMSRLMPALGKAQPPHNGSDARSSRNEHRKSKSSGLRGESGANVEMQRQRFRQFRYQDSEGPREACRRLRELCRRWLKPESRTKEQILELLILEQFLTILPQEIQNWVWERGPETCAQAVALVEGYQMGRREAGMWEQQVTVRVKVEQVSPQEMILPGAPWDPSASLLPHPEYLSSSDLTLNQPVPGPALKMPCIPKQEPQGLQETAQEDIIWMELGGSLEGVRQGSLLANGNREEHSLPGGTMEMGLLPGNSREKVLGENCGERMGQQFEVRCGDTRENPVAHPNSFGRSHPAGKSLHQCSQCGKTFSRNSHLLTHLRIHTGEKPHQCPQCGKRFGHTSQLTRHQRTHASERPFRCAQCSRSFYQSSELTRHRVSHARDRPYGCSQCGKKFRWSSDLIRHQITHTGERPYKCPDCGKKFGQNSHLVRHRRTHTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X958</accession>
    <name>A0A6I9X958_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium-dependent lysophosphatidylcholine symporter 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106539890</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910282.1">
        <property type="nucleotide sequence ID" value="XM_014054807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X958"/>
    <dbReference type="GeneID" id="106539890"/>
    <dbReference type="KEGG" id="tsr:106539890"/>
    <dbReference type="OrthoDB" id="197206at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015245">
        <property type="term" value="F:fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051978">
        <property type="term" value="F:lysophospholipid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990379">
        <property type="term" value="P:lipid transport across blood-brain barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140329">
        <property type="term" value="P:lysophospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328:SF29">
        <property type="entry name" value="SODIUM-DEPENDENT LYSOPHOSPHATIDYLCHOLINE SYMPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910282.1"/>
        </source>
    </evidence>
    <sequence length="106" mass="11939" checksum="D081BC0D6BA4FC79" modified="2020-10-07" version="1">MALLRTLSGPWRFLKRESKTKLSICSKVCYAIGGAPYQITGCALGFFLQIYLLDVVQMDPFYASLILFGGRAWDAITDPLVGFFISKSSWTCFGRLMPCLCHKSHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9K8</accession>
    <name>A0A6I9X9K8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">RING-type E3 ubiquitin-protein ligase PPIL2</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">CYC4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Probable inactive peptidyl-prolyl cis-trans isomerase-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC106540423</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Has a ubiquitin-protein ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation. May also function as a chaperone, playing a role in transport to the cell membrane of BSG/Basigin for instance. Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the minor spliceosome, which splices U12-type introns. Within this complex, interacts with PRPF8/PRP8, EFTUD2/SNU114 and PLRG1. Interacts with isoform 2 of BSG. Interacts (via the PPIase cyclophilin-type domain) with CRNKL1; they may form a trimeric complex with HSP90.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013910996.1">
        <property type="nucleotide sequence ID" value="XM_014055521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9K8"/>
    <dbReference type="GeneID" id="106540423"/>
    <dbReference type="KEGG" id="tsr:106540423"/>
    <dbReference type="OrthoDB" id="30774at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01923">
        <property type="entry name" value="cyclophilin_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16663">
        <property type="entry name" value="RING-Ubox_PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000079">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000018">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026951">
        <property type="entry name" value="PPIL2_U-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN-PROTEIN LIGASE PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="23">
        <location>
            <begin position="35"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="22">
        <location>
            <begin position="285"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="446"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="447"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00073734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013910996.1"/>
        </source>
    </evidence>
    <sequence length="519" mass="58759" checksum="332A2B2A5E907298" modified="2020-10-07" version="1">MGKRQHQKDKMYITCTEYTHFYGGKKVEIPQSNFRRLPFDHCSLSLQPFEYPVCTPDGIIFDLLNIVPWIKKYGTNPITGEKLEAKSLIKLNFAKNNDGKYHCPVLFTVFTNNSHIVAIKTTGNVFGFEAVEQLNIKTKTFKDLLSDEPFSRPDIITLQDPTNVDKFNVSSFFHVKNNLKVTDPDDEKARSDPSYYLKNANIETKETLSELYKEFKGDNILAATMKEPEKKKTDRLNAAHYSTGRVSASFTSTAMAPETTHEAAAIDDDVVRYRYVKKKGYIRLHTNKGDLNLELHCDMTPKTCENFIKLCKKNYYDGTIFHRSIRNFMIQGGDPTGTGTGGESYWGKPFKDELRPNLSHKGRGILSMANTGPNSNKSQFFITFRSCAYLDKKHTIFGRVVGGFETLTAMENVESDPKTDHPKEEIKIKSTTVFVDPYEEADAQITQEREKAQQEEEAIKRESKEVRKKEPPTPKTYHTGVGKYINIAAAKRAAEDGPSTSGVAKKEKKTGGFGDFSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9P8</accession>
    <name>A0A6I9X9P8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hepcidin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106538108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the hepcidin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907978.1">
        <property type="nucleotide sequence ID" value="XM_014052503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9P8"/>
    <dbReference type="GeneID" id="106538108"/>
    <dbReference type="KEGG" id="tsr:106538108"/>
    <dbReference type="OrthoDB" id="9428792at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010500">
        <property type="entry name" value="Hepcidin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06446">
        <property type="entry name" value="Hepcidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026759337" evidence="6">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013907978.1"/>
        </source>
    </evidence>
    <sequence length="81" mass="9094" checksum="72694634A63AF534" modified="2020-10-07" version="1">MKLQVICVLFVLLSAATRNLCAFTIQRDIQKDLAGADTYKTESMGLQALLGRSKRHISHIALCRYCWDCCGNKGFGYCCRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XA29</accession>
    <name>A0A6I9XA29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-specific chaperone E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Tubulin-folding cofactor E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">TBCE</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin-folding protein; involved in the second step of the tubulin folding pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908097.1">
        <property type="nucleotide sequence ID" value="XM_014052622.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908098.1">
        <property type="nucleotide sequence ID" value="XM_014052623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XA29"/>
    <dbReference type="GeneID" id="106538192"/>
    <dbReference type="KEGG" id="tsr:106538192"/>
    <dbReference type="CTD" id="6905"/>
    <dbReference type="OrthoDB" id="5273213at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17044">
        <property type="entry name" value="Ubl_TBCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000008">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000268">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001017">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044079">
        <property type="entry name" value="Ubl_TBCE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="12">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013908097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013908098.1"/>
        </source>
    </evidence>
    <sequence length="531" mass="60253" checksum="8E4747B90AD2A7DD" modified="2020-10-07" version="1">MSDTIPSDAVGRRILCNGEYGTVLYVGNVPPTTGIWLGVEWDNHERGKHNGSHEGIQYFECRHPTGGSFIRPNKANFGVDFLSAVKDKYGVNENEHNPESETGMPLVFGRKTVETVGFDSIKKKQKQLNTLRDISVDRCAVSLAGQEEEIRKSCPNIRQINLSRNLLSSWKEVLAIACQVENLETLNISENKMKFPSVLPPASQTFCKLRILALNQTGVTWTEVLLCATGWPALEELYLASNSISLLKRPIDVLQNLKWLDLSNNQLTDENQLYLIADLPRLERLILSNNGIYSMHFPDAAFGNKTRMFPSLTHLILKDNKIAKWSVINELDKLQKLESFDCRNNPLMDSENNARTLEQLIIAKIGQLKFLNNSQIFPEERRGAELDYRKKFGIHWLKAGGNQDPKKNKPSEEFLAAHPRFQLLCDLYGAPEDGELERKQSFSLKNQLLTLTFKCPDKADQKPIEKKLPDSMTIQKVKGLLYRLLKIPGSELKLSYESSKVSSNSVKQVYELRRSRIIQLITLVKSIHCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XB03</accession>
    <name>A0A6I9XB03_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Astrotactin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106540424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910997.1">
        <property type="nucleotide sequence ID" value="XM_014055522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XB03"/>
    <dbReference type="GeneID" id="106540424"/>
    <dbReference type="KEGG" id="tsr:106540424"/>
    <dbReference type="OrthoDB" id="9011270at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043533">
        <property type="term" value="F:inositol 1,3,4,5 tetrakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007158">
        <property type="term" value="P:neuron cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040685">
        <property type="entry name" value="Annexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040510">
        <property type="entry name" value="ASTN_2_hairpin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026995">
        <property type="entry name" value="Astrotactin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16592">
        <property type="entry name" value="ASTROTACTIN-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16592:SF2">
        <property type="entry name" value="ASTROTACTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18411">
        <property type="entry name" value="Annexin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18577">
        <property type="entry name" value="ASTN_2_hairpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Annexin-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Astrotactin-2 C-terminal beta-hairpin" evidence="2">
        <location>
            <begin position="61"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013910997.1"/>
        </source>
    </evidence>
    <sequence length="114" mass="13423" checksum="1D1A70C7CF6B6597" modified="2020-10-07" version="1">MEVSASMLSRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRRLEKVSNHCSNLLRSAYIQSRTDTMPYLFCRSEEERSPGMVCYNVLKDTKISCEEKMISLLRNMYGDSKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCQ4</accession>
    <name>A0A6I9XCQ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106540872</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013911632.1">
        <property type="nucleotide sequence ID" value="XM_014056157.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540872"/>
    <dbReference type="KEGG" id="tsr:106540872"/>
    <dbReference type="OrthoDB" id="9906564at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000063">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF12">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="11">
        <location>
            <begin position="204"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="12">
        <location>
            <begin position="751"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911632.1"/>
        </source>
    </evidence>
    <sequence length="995" mass="110385" checksum="C1AE380F9B96EB84" modified="2020-10-07" version="1">MLCLTPAVIHVTSSTNAILRNSNHDLSCHLLQKKIQLTVTLEGKPCEMELDTGSATSIVSWSTIKRLLPQLSKRQLQDCHLTLRDYQGNLIPVIDTRKVRVQFKGFDGRLPLIIVEGRLPSLLGLDWFQSLGLQISGIHHLSATALDCXRQRVPAVFDGTLGKYTGTPVSFNLDPSVQPVRMKPRXVPIALKPKVDAELNRLVAQGILEPVDHARWETPIVLPIKPDGSMRICADYRCSINRALPSNAYPVPVVQHLLHTLGEGTVFAKLDLAQAYQQLVVDDEAAEAQTIITHRGAFHCRRLQFGVSIAPGLFQSLMERLLQGLRGVVPYFDDVLVSATSMDELMARPRLVLQRFQQVGLKVKRDKCQVAVSQIEFLGFLIDGSGIHPTAAKTKAIVEAPAPTNRAELQAFLGLLNFYAVFLPHKATVAEPLHRLLDSGAPWVWGRREASAFLAVKHLLISNDVLVQYSEHLPLVLACDASPYGVGAVLCHQLPSGAEVPVAYFSRTLSASERNYAQIDKEALAIVAGVRRFHHYLYGRPFQIVTYHKPLLGLLAGDRPAPQVLSPRMTRWFVFLASYEYQLKHRPGKQIAHADALSRCPLPEALEDPAAAAPVFLIDDLNLPLSAADIARESARDQVISRVLDWVRRGWPRDPVDPVFLPFKSRLAELSVQRGCLLWGHRVVVPASLRTTVLQQLHYAHPGIVRMKALGRSYVWWPKLDQDIADHVAGCSQCQSAQALPPKAEPRTWEPPSTPWSRVHVDFAGPIQGRMLFIAVDAFSKWVEVVHMSSTMADALIAILRHLFATHGLPDVLVSDNGPQLTATKFEAFLASLGIRHALTSPFHPSSNGLAEHAMRSVKEALAKMDHLPWQERIDAFLLAQHSTPSPDSNTSPAELLMGRRLRTTLDRLHPAYSPPTPLDSQGGSRSFVQGEAVYARNYGGHPLWLPGVVTDQTGPCSYRFEDEHNVKIVNKEQLIYVIKIKVDYSNKPISHEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDA6</accession>
    <name>A0A6I9XDA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Hexokinase-2</fullName>
            <ecNumber evidence="6">2.7.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Hexokinase type II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC106541043</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Monomer. Interacts with TIGAR; the interaction increases hexokinase activity in a hypoxia- and HIF1A-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013911842.1">
        <property type="nucleotide sequence ID" value="XM_014056367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDA6"/>
    <dbReference type="GeneID" id="106541043"/>
    <dbReference type="KEGG" id="tsr:106541043"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24089">
        <property type="entry name" value="ASKHA_NBD_HK1-2_meta_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24091">
        <property type="entry name" value="ASKHA_NBD_HK1-3_meta_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000015">
        <property type="entry name" value="Hexokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000001">
        <property type="entry name" value="Hexokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000020">
        <property type="entry name" value="Hexokinase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000805">
        <property type="entry name" value="Hexokinase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF4">
        <property type="entry name" value="HEXOKINASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="26">
        <location>
            <begin position="22"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="27">
        <location>
            <begin position="226"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase N-terminal" evidence="26">
        <location>
            <begin position="471"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="27">
        <location>
            <begin position="674"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013911842.1"/>
        </source>
    </evidence>
    <sequence length="917" mass="102525" checksum="C67EFD16EFB8F685" modified="2020-10-07" version="1">MIASHLLAYLFTELHHDQAQRVDKYLYHLRLSDDTLRDISERFRKEMEKGLGADTNPTASVKMLPTFVRSIPDGTEEGDFLALDLGGTNFRVLRVKVADNGSKKVEMENQIYDIPEELMRGSGMQLFDYIADCLANFMEQLQIKDRKLPLGFTFSFPCHQTKLDESILDTWTKGFKCSGVEGKDVVSLLRTAIKKRGDFEIDVVAVVNDTVGTMMTCGYDDQNCEVGLIIGTGSNACYMEEMRHIETVEGDEGRMCINMEWGAFGDDGSLKDIRTEFDQEIDMGSLNPGKQLFEKMISGLYMGELVRLILVKMAKEKLLFNGKVPDNLRKKGCFETRYVSAIEKEKEGISKARTILQQLGLEPSHEECVATQRVCEIVSTRSASLCGAALAAVLQRLKENRGVNWLRTTVGVDGTVYKKHPHFARRLHKVVRHLLSGHEIRFVRSEDGSGKGAAMVTAVAYRLAAQHKARQKILHPLRLSQQQLLEVKRRMQREMELGLAQKTHTEATVKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRIRNGMRRSVEMHNKIYTISEDVMQGTGEELFDHIVSCISDFLEYMGMKGASLPLGFTFSFPCQQKRLDEGILLQWTKGFRASGCEGEDVVGLLKEAILRRKECELDVAALVNDTVGTMMSCGYEDPLCEVGLIVGTGTNACYMEELRNVELVAGDQGRMCVNMEWGAFGDNGCLDYLWTEFDTTVDENSLNPGKQRFEKMISGMYLGEIVRNILIDFTKKGLLFRGRISERLKTKGIFKTKFLSQIESDCLALLQVRHILQDLGLKSTCDDSIIVKEVCTVIARRAAQLCGAGVAAVVDKIRENRHLDSLKVTVGVDGTLYKLHPHFSKVMQETVKQLSPKCEVSFLQSEDGSGKGAALIAAVACRLREAGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XE27</accession>
    <name>A0A6I9XE27_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ubiquitin carboxyl-terminal hydrolase 39</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">U4/U6.U5 tri-snRNP-associated 65 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">USP39</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">The U4/U6-U5 tri-snRNP complex is a building block of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013913884.1">
        <property type="nucleotide sequence ID" value="XM_014058409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XE27"/>
    <dbReference type="GeneID" id="106542626"/>
    <dbReference type="KEGG" id="tsr:106542626"/>
    <dbReference type="CTD" id="10713"/>
    <dbReference type="OrthoDB" id="10263353at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02669">
        <property type="entry name" value="Peptidase_C19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000068">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000030">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033809">
        <property type="entry name" value="USP39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="16" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="25">
        <location>
            <begin position="70"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="24">
        <location>
            <begin position="192"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013913884.1"/>
        </source>
    </evidence>
    <sequence length="533" mass="61868" checksum="67A146DE289515CA" modified="2020-10-07" version="1">MAARVKREPRSPQPWLGCPTVGWARVKRESAEEVAIKQEPVEPEEEEDEDGAEEPTVKYGRFDPEDRRSRHCPYLDTINKSVLDFDFEKLCSISLSHINVYACLVCGKYFQGRGLKSHAYIHSVQFSHHVFLNLHTLKFYCLPDNYEIIDSSLEDITYVLKPTFAKQHITNLDRQAKLSRAYDGTTYLPGIVGLNNIKANDYANAVLQALSNVPPLRNYFLEEENYKDIKRPPGDIMFLLVQRFGELMRKLWNPRNFKAHVSPHEMLQAVVLCSKKNFQITKQGDGVDFLSWFLNALHSALGGTKKKKKTIVTDVFQGSMRIFTKKLPHPDLPAEEKEQLLQNNEYQEKMVESTFMYLTLDLPTAPLYKDEKEQLIIPQVPLFNILAKFNGIMEKEYKTYKENFLKRFQLTKLPPYLIFCIKRFTKNNFFVEKNPTIVNFPITNVDLREYLSEEVQAVHKNTIYDLVANIVHDGKPTEGAYRIHVLHHGTGKWYELQDLQVTDILPQMITLSEAYIQIWKRRDSQEASHQQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEM6</accession>
    <name>A0A6I9XEM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Cysteine/serine-rich nuclear protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">CSRNP3</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AXUD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912316.1">
        <property type="nucleotide sequence ID" value="XM_014056841.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912317.1">
        <property type="nucleotide sequence ID" value="XM_014056842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEM6"/>
    <dbReference type="GeneID" id="106541398"/>
    <dbReference type="KEGG" id="tsr:106541398"/>
    <dbReference type="CTD" id="80034"/>
    <dbReference type="OrthoDB" id="5946974at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031972">
        <property type="entry name" value="CSRNP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023260">
        <property type="entry name" value="Cys/Ser-rich_nuc_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13580:SF13">
        <property type="entry name" value="CYSTEINE_SERINE-RICH NUCLEAR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13580">
        <property type="entry name" value="TGF-BETA INDUCED APOPTOSIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16019">
        <property type="entry name" value="CSRNP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02031">
        <property type="entry name" value="CYSSERRICHNP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cysteine/serine-rich nuclear protein N-terminal" evidence="10">
        <location>
            <begin position="61"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="335"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="374"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013912316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013912317.1"/>
        </source>
    </evidence>
    <sequence length="601" mass="67266" checksum="08914249E785D4AA" modified="2020-10-07" version="1">MSGILKRKFEEVDGSSPCSSVRESDDDISSSESAESGDSVNPSTSNHFTPSSILKSEKRKRTKNVHFNCVTVYYFTRRQGFTSVPSQGGSTLGMSTRHNNVRQYTLGEFAMEQERLHQEMLREHLRQEKLNSLKLKMTNNGTVESEEANTLTLEDISDDDIDLDNTEVDEYFFLQPLPTKKRRALLRASGVKKIDVEEKHELRAIRLSREDCGCDCRIFCDPETCTCCLAGIKCQVDRMSFPCGCTKEGCSNTAGRIEFNPIRVRTHFLHTIMKLELEKNREQQVPALNGCHAELNAHSSAMGPTSHPLEFSVPENFELETEPRAAVMHLQSADDLDCAGEEEEEEEEEEEEEDASSFCSGVTDSSTQSLAPSETDEDEEEEDDEEEDDEEEEDEEEEEEEEEKLDECLEGLGVHADAGQALPSVLCYSDSTAMHENQPKALSYYPSSTSLYYQIENHSPGTPNQIRETYSERETVKNGNLSLVPYNPTSEPFEDYTRPSEENYGSSLYPSSNPSVIVCCSSSESDNVVPCSSLYAEHRPRHSQVDFPAYLKSPSQDGFVSALNGGACLPEHPTENSLGLSEKSRLHEECIKSPVMETVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGI4</accession>
    <name>A0A6I9XGI4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Arylsulfatase H-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106543328</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914794.1">
        <property type="nucleotide sequence ID" value="XM_014059319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGI4"/>
    <dbReference type="GeneID" id="106543328"/>
    <dbReference type="KEGG" id="tsr:106543328"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.10:FF:000001">
        <property type="entry name" value="Arylsulfatase E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000233">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.550">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF5">
        <property type="entry name" value="ARYLSULFATASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="36"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013914794.1"/>
        </source>
    </evidence>
    <sequence length="583" mass="65352" checksum="9B5C29ED8B250F25" modified="2020-10-07" version="1">MKLFNKFSGGNASCSPTVLLVSILFSQTWVTSMSKPNILILMADDLGTGDLGCFGNETMRTPNIDQLAKEGVKLTQHISASSLCTPSRSAFLTGRYPIRSGMAATTERRVKAGPGTTGGLPTNETTFATLLQQQGYTTGIIGKWHQGLNCESRNDHCHHPLNNGFDYFYGDPFTLVNDCLSWEYSELEPGNKNPYYVQIVAVLLLTLLVGKIIGLVSLKWKIVFIIILCGIFYITYTFVHYLFRKSWNCLMFRNHEIVQQPLKLEDAAFRIVREAVSFIERNKKRPFLLFVSFLHVHTPCYSTSLFHGTSKHGLYGDNVEEMDWMVGNILAALDKEGLKNDTFTYFTSDNGGRREVFQWGERLGGWNGKYKGGKGMGGWEGGIRVPGIVRWPGKVAPNTVLDEPTSLMDIYPTVAHLAGASVPQDRIIDGKNQIELLLGTVQHSAHEFMFHYCGTHLHAVRWHEKESGSLWKIHYVTPNFQPKGADGCYGKIMCPCSGDNVTYYNPPLVFDLLRDPSESTPLSPISELHFDIIISQAEEAVREHQLTVTHVPNQLDLPSNQWEKNLQPCCGIFPFCCCDKETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XH32</accession>
    <name>A0A6I9XH32_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RALB</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013915014.1">
        <property type="nucleotide sequence ID" value="XM_014059539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XH32"/>
    <dbReference type="GeneID" id="106543502"/>
    <dbReference type="KEGG" id="tsr:106543502"/>
    <dbReference type="CTD" id="5899"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04139">
        <property type="entry name" value="RalA_RalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000203">
        <property type="entry name" value="Putative ras-related protein ral-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915014.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="23295" checksum="46B3E647F199A57B" modified="2020-10-07" version="1">MAASKAKSQSSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEDRRQVPVEAARSKAEEWGVQYVETSAKTRANVDKVFFDLMREIRSKKMSDNKDKNGKKSSKNKKSFKERCCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHA7</accession>
    <name>A0A6I9XHA7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dystonin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106543571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915094.1">
        <property type="nucleotide sequence ID" value="XM_014059619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHA7"/>
    <dbReference type="GeneID" id="106543571"/>
    <dbReference type="KEGG" id="tsr:106543571"/>
    <dbReference type="OrthoDB" id="10016565at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF34">
        <property type="entry name" value="KH DOMAIN-CONTAINING, RNA-BINDING, SIGNAL TRANSDUCTION-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915094.1"/>
        </source>
    </evidence>
    <sequence length="248" mass="28801" checksum="20C5F319A04CD564" modified="2020-10-07" version="1">MKEKMKKFMETADPSTASSLEAKMNELSKRFCEAHNKHKKKLEDMEKLKTRVELFECLSDKLQSFFDKKTQTLNEADIPGKDVAEMFLCVQETNTELMEQKKDLEVLQHLIEELSPHALPGDKSLVLEKVNVLSKKFREMEEMIQEKEKDVSSCQQQIDAFRGYVDSLKKWIDETTERIPPIQPSLNTDSLKKHLQSTKEEELRKSEEAKYAHLSDELHVLIEVFAPPGEAYSRMSHALEEIKKFLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ36</accession>
    <name>A0A6I9XJ36_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Afadin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106542759</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914051.1">
        <property type="nucleotide sequence ID" value="XM_014058576.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542759"/>
    <dbReference type="KEGG" id="tsr:106542759"/>
    <dbReference type="OrthoDB" id="6260541at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22711">
        <property type="entry name" value="FHA_AFDN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15471">
        <property type="entry name" value="Myo5p-like_CBD_afadin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06789">
        <property type="entry name" value="PDZ_AFDN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000032">
        <property type="entry name" value="Afadin isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000006">
        <property type="entry name" value="Afadin, adherens junction formation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028842">
        <property type="entry name" value="Afadin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037977">
        <property type="entry name" value="CBD_Afadin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10398">
        <property type="entry name" value="AFADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10398:SF2">
        <property type="entry name" value="AFADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="3">
        <location>
            <begin position="264"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="610"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="908"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="967"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1181"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1219"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1356"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="755"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="916"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="933"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="973"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1189"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1230"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1317"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1333"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1392"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1406"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1435"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1462"/>
            <end position="1473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013914051.1"/>
        </source>
    </evidence>
    <sequence length="1518" mass="171995" checksum="964A45A5356C4634" modified="2020-10-07" version="1">MIKLSVATDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQSHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGTSHVFKFVDPIQDHIPKGRLDAGHMVKSSRLKSGGVQETTFDLEGDIQSVTGLSTSKSINRLDCDRMSTSSTAERSMVKPMIRIEQQDYHKQDGRLQDGPELILPASVEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSSQYRPDISPAERTHKVIAIVNKMVKMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENNPQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSPEDIPNINSTCFKLNSLQLHALLQNYHCAPDEPFIPPELIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRAFCRLTPHPRSPGTWTIYFEGADYESHLLQDNADLAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGTVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWTEYNEPKKLPGDDRLLKNRADHRSSPNVANQPPSPGTKKAYTGGTTNKITSVSTGNLCTEEQVLPARPEAYPIPTQTYTREYFTFPASRSQDRMGPIQNQWANYEEKPQIPTESNHYINNANQRVTRSEEELRDDNIYQAERNQLEVAMQKDMGYIERKSDSDPWMNSSVDSSTSSQEHLNSSSKCISSGSSLTKSGPGRWKTPIITQPMLLAVSQSTRTDLPPPPPPPPMHYVADFDGLQMDLPLPPPPPPANQLGSQSSQVAAAAERKKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRAQPLMPAQSLPPPLQQVKPEKPSTLQRSHETVIRELQPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQNKPDRTGEENDRLRKLMLEWQFQKRLQESKQKDEDEDEEEDDDIDTMLIMQRLEAERRVRQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERCRQEEERTKREAEQKRRQEEEYYNRLEAERRRQHDEADRRLLEPDEPGLYRPPLPREYEFPSPPLSSNAPPPPPQRNTSYLKTQVLSPDTIYTAKFVAYNDEEEEDSCLATGQDKYTSTRKSCGDLPPVPPKPQQPSRQPRPVSDGLCLSNSYQPSAVNANSTANKMSQPPPPPQKPSFINPSNTKGSNYTGSTGALAGSQESYSDPREKKLKCQDTDIPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XK30</accession>
    <name>A0A6I9XK30_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 154</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMEM154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914366.1">
        <property type="nucleotide sequence ID" value="XM_014058891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XK30"/>
    <dbReference type="GeneID" id="106543003"/>
    <dbReference type="KEGG" id="tsr:106543003"/>
    <dbReference type="CTD" id="201799"/>
    <dbReference type="OrthoDB" id="9451445at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028064">
        <property type="entry name" value="TMEM154"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053087">
        <property type="entry name" value="TMEM154-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36526">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36526:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15102">
        <property type="entry name" value="TMEM154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914366.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="20728" checksum="8A228F23BC69B38E" modified="2020-10-07" version="1">MADHEQSGDGILSTGVPEVGFTVLFSTSANKSEEQNATTPIKTEDVVMKSTQTPEMNENEPILHPAVIIGVSIVLILVLLIILVIFIVRCRKQKQSQEDELGSENVKRSISPSQQVLSSSPTISPLPIFEEDTPSVMEIEMEELDKWMNSLKKNESDYLPPVKEEKDGNTNPRLTMLNGNFDSCNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLX5</accession>
    <name>A0A6I9XLX5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hepatocyte growth factor-regulated tyrosine kinase substrate</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HGS</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
            <location evidence="1">Multivesicular body membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916854.1">
        <property type="nucleotide sequence ID" value="XM_014061379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLX5"/>
    <dbReference type="GeneID" id="106544955"/>
    <dbReference type="KEGG" id="tsr:106544955"/>
    <dbReference type="CTD" id="9146"/>
    <dbReference type="OrthoDB" id="957735at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15720">
        <property type="entry name" value="FYVE_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21387">
        <property type="entry name" value="GAT_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03569">
        <property type="entry name" value="VHS_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1940:FF:000003">
        <property type="entry name" value="Hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000014">
        <property type="entry name" value="Hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000028">
        <property type="entry name" value="Putative hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017073">
        <property type="entry name" value="HGS/VPS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024641">
        <property type="entry name" value="HRS_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46275">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR-REGULATED TYROSINE KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46275:SF1">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR-REGULATED TYROSINE KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12210">
        <property type="entry name" value="Hrs_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036956">
        <property type="entry name" value="Hrs_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="14">
        <location>
            <begin position="15"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="13">
        <location>
            <begin position="160"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="223"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="627"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="694"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="450"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="637"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="694"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="729"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013916854.1"/>
        </source>
    </evidence>
    <sequence length="761" mass="85042" checksum="FB057DDC3158ECB0" modified="2020-10-07" version="1">MGRGGGTFERLLDKATSQLLLETDWESILQICDMIRQGDTQAKYAVGAIKKKVNDKNPHVALYALEVLESVVKNCGQTVHDEVANKQTMEELKELFKRQVEANVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEHLNKKGEGKGTATTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLAIALSQSEAEEKERMRQKSTYSTYPKAEPTPITSSAPPGGTLYSSPVNSSAPLAEDIDPELARYLNRNFWEKKQEEVRKSPTPSAPLSMGEPATQTTEVPPAPLSVVEQQYQNGETDESHEQFLKALQTAVTTFVNRMKSNHLRGRSITNDSSVLSVFQSINSMHPQLLELLNQLDERRLYYEGLQDKLAQIRDARGALNALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEMQRQLAIQRLQEQEKERQLRLEQQKQTIQMRAQMPAFSLPYAQLQAMPPASGVIYQPSGPTSFPGTFSPASSVEGSPMHTVYMSQSAQGSTGPYTAMPVAGTDPGMVSTYMYSTGNNNTQPAPQAQANPAYSSYQPTPTQGYQNAPAPSQTQTVPAMTQAPPQSGGTMGYMGNQSMPMGYQPYNMQNLMSTLPGQESGLSSLPPQQPYLSGQQPMYQQMAPSGGPPQQQPPPQQTSSQVQQVQGGSGEAQLISFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM67</accession>
    <name>A0A6I9XM67_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proton-translocating NAD(P)(+) transhydrogenase</fullName>
            <ecNumber evidence="3">7.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NNT</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>NAD(+) + NADPH + H(+)(in) = NADH + NADP(+) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the AlaDH/PNT family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013915026.1">
        <property type="nucleotide sequence ID" value="XM_014059551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM67"/>
    <dbReference type="GeneID" id="106543516"/>
    <dbReference type="KEGG" id="tsr:106543516"/>
    <dbReference type="CTD" id="23530"/>
    <dbReference type="OrthoDB" id="37244at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:proton-translocating NAD(P)+ transhydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05304">
        <property type="entry name" value="Rubrum_tdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000028">
        <property type="entry name" value="NAD(P) transhydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008143">
        <property type="entry name" value="Ala_DH/PNT_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026255">
        <property type="entry name" value="NADP_transhyd_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024605">
        <property type="entry name" value="NADP_transhyd_a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034300">
        <property type="entry name" value="PNTB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00561">
        <property type="entry name" value="pntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006942">
        <property type="entry name" value="PRK09424.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160">
        <property type="entry name" value="NAD(P) TRANSHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160:SF22">
        <property type="entry name" value="NAD(P) TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02233">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12769">
        <property type="entry name" value="PNTB_4TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00837">
        <property type="entry name" value="ALADH_PNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="526"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="621"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="645"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="671"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="735"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="802"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="857"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="890"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="920"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="13">
        <location>
            <begin position="59"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="12">
        <location>
            <begin position="207"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915026.1"/>
        </source>
    </evidence>
    <sequence length="1135" mass="121154" checksum="8BC2195C3EC66D7E" modified="2020-10-07" version="1">MAGILRTVSNFSVPLFNNVICHKVKTVKTPRVRTFLTHQVLWCKAATKSGIPYKELTIGVPKEIFQNEKRIALSPAGVQALVKQGFNVIVESSAGEAAKFSDDHYREAGAQIQGTKEVLASDIVLKVRAPMFNSVLGIHEIELFKPHGTLISFIYPAQNPDLLNKLAEKNTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVILAANNFGRFFTGQITAAGKVPPAKVLIIGGGVAGLASAGAAKSMGAVVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAKQCQEVDIIITTALIPGKKAPILFRKDMIELMKEGSVVVDLAAEAGGNIETTKPGELYVHKGITHIGYSDLPSRMATQASTLYSNNITKLLKAISPDKENFYFHIKDEFDYGTLDHVVRGTVVMKDGKVIFPAPPPKNIPQAAPVKQKTVAELEAEKAATITPFRKTMTSASAYSAGIASLLGLGIASPNSAFTQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLALMGGEYLPGTLPQGLAVLAAFISSVNIAGGFLVTQRMLDMFKRPTDPPEFNYLYLLPAALFIGGYGTALQSGYNIEQMMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGSLKPSVELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCVAEYMIEFPHFATDPAAGLTMIVAYLGTYIGGVTFSGSLVAYGKLQGILNSAPLLLPGRHLLNASLLTLSIGGMVPYMMDPSYTTGLTCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSHIMCVVRRFWRCPFQHYLFHYIFSACGKSFLLYILFSFWAFSASSFSPFFPLLIFARTYLLSKIGTFINVANQFRWRTTLFFLPCDYLMFNANNIIESHLITEVYLSPLEASFISENQDNIKQPLESTCNATYAEKFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINEDFPETDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKAKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDGLQAKMREFCPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMW6</accession>
    <name>A0A6I9XMW6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydropyrimidinase-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106545291</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917279.1">
        <property type="nucleotide sequence ID" value="XM_014061804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMW6"/>
    <dbReference type="GeneID" id="106545291"/>
    <dbReference type="KEGG" id="tsr:106545291"/>
    <dbReference type="OrthoDB" id="10258955at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016812">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.40.10:FF:000021">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000174">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF56">
        <property type="entry name" value="DIHYDROPYRIMIDINASE-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="10">
        <location>
            <begin position="161"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013917279.1"/>
        </source>
    </evidence>
    <sequence length="481" mass="52304" checksum="9BA6B64F44ED7C86" modified="2020-10-07" version="1">MAEKKQAAKPGGEEEEEVPAFFKNLGSGSPKPRQKFCGMFCPVEGSLENKTIDFDSLSSGRGSPTGKIVAKQRDVAHLGPDAPSVSSWPGRKVEIRRTAGKEALQNLNDKSDRLLIKGGKIVNDDQSFYADIYMEDGLIKQIGENLIVPGGVKTIEAHGRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTRAALAGGTTMIIDHVVPEPGTSLISAFDQWREWADSKSCCDYSLHVDITEWHKGVQEEMEALIKDHGVNSFLVYMAFKDHFQLTDSQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILELGITGPEGHVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSAAEVIALARKKGTVVYGEPIAASLGTDGSHYWSKNWAKAAAFVTAPPLSPDPTTPDFLNSLLSCGDLQVTGSGHCTFNTAQKAVGKDNFTLIPQGTNGTEERMSIIWDKAVVGCFGLDWDQAYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XN96</accession>
    <name>A0A6I9XN96_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nuclear receptor-binding factor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NRBF2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915371.1">
        <property type="nucleotide sequence ID" value="XM_014059896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XN96"/>
    <dbReference type="GeneID" id="106543813"/>
    <dbReference type="KEGG" id="tsr:106543813"/>
    <dbReference type="CTD" id="29982"/>
    <dbReference type="OrthoDB" id="3694230at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039679">
        <property type="entry name" value="NRBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015056">
        <property type="entry name" value="NRBF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033393">
        <property type="entry name" value="NRBF2_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14964">
        <property type="entry name" value="NUCLEAR RECEPTOR BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14964:SF2">
        <property type="entry name" value="NUCLEAR RECEPTOR-BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08961">
        <property type="entry name" value="NRBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17169">
        <property type="entry name" value="NRBF2_MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140361">
        <property type="entry name" value="MIT domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear receptor-binding factor 2 MIT" evidence="4">
        <location>
            <begin position="10"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear receptor-binding factor 2 C-terminal" evidence="3">
        <location>
            <begin position="96"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013915371.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="33960" checksum="9225F2FC1A3631FE" modified="2020-10-07" version="1">MSPQPPMEAMEAPLNLAHQQSRKADHLLAAGKYEEAVSCHRKAAAYLLEAMKLTQSDQAQLSLELQRKSHLKQIPLIQERWKRTQRDERLKVRPLVDKESVAHLQSSSKSSAEDPEDQHGTPASLKYRAPLEKEMKGFQGVLDRDPDTLLFLLQKRKEPSPDACVKSRPPKDDKMKMEEQATKIAVLEQHVEMLFADNERLRRENRQLKAERARLLKSPLEKELDADADFVEKSELWGLQQLPESAAASAATTTTPWQKLAVSAGKGKDIPIPSLPPLDIPSPEFPLVELSEDLLKGFMNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNX1</accession>
    <name>A0A6I9XNX1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-binding cassette sub-family A member 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106544047</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915667.1">
        <property type="nucleotide sequence ID" value="XM_014060192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNX1"/>
    <dbReference type="GeneID" id="106544047"/>
    <dbReference type="KEGG" id="tsr:106544047"/>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF100">
        <property type="entry name" value="CHOLESTEROL TRANSPORTER ABCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="429"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013915667.1"/>
        </source>
    </evidence>
    <sequence length="775" mass="88346" checksum="0275C761D8DD1D9F" modified="2020-10-07" version="1">MFVVHHYVKKAVVPIQLSPNLYFLKTNETYDKYKTSLLIQNSTGSNIDDLISALRNQNILTEIRSDSDYESADFHSSSLNNSGYVSFAPHAAALNVFKVDKNYIFTAAFNRTMIHSLPVMMNIISNFYLYSSNITEKIQVWSNPFFQEITDTVFTLVLYFEAVLLGVLVTGMPSYFAMENAENHKIRAYTQLKISGLYPSAYWCGQALIDIPLFYIILLLMIGSLFVFHHGIYFFPEKFLAVVFCLIGYVPSVIMFNYIVSFSFNAIQNTKEFWSFIFSVAALVCSVVIEVAFFMWYYTTAAVLHACFSIFVPIYPLIGCLISFIKIVRKLDNKMEKHSERLLISVVAPYLQCVVWLFLLRYLEMKCGGKSVRKDPFFRTPMKPKSWKHPDVPHDENEDEDVTAERLRVKELITCQSCEEKPAVLVHCLHKEYDEKSDFLLGRKIKKVATNCVSLCVKKGEILGMLGPNGAGKSTLINILVGEVEPNSGQVLMGDFSSSDNNEDDSMRFVGYCPQINPLWPEITLQEHFEIYGAIKGMSESDLKEVIKCISSALDFKEQLQKKVKKLGAGIKRKLCFALSMLGSPHITLLDEPSTGMDPKAKQHMWKAIRAAFKSKKRAAILTTHYMEEAEAVCDRVAILVSGKLRCIGTVQHLKTKFGKGYFLEMKLREVPDLQMKEQLQRAVLRIFPNGRPQESFGSISSYKIPKEDVHSLALSFSKLEEAKHAFNIEEYSFSQATLEQVFVELAKEQEEEDSFGTLNSMLWWERTQGERVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP20</accession>
    <name>A0A6I9XP20_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Complement C5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106545625</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917739.1">
        <property type="nucleotide sequence ID" value="XM_014062264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP20"/>
    <dbReference type="GeneID" id="106545625"/>
    <dbReference type="KEGG" id="tsr:106545625"/>
    <dbReference type="OrthoDB" id="6359008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048843">
        <property type="entry name" value="C5_CUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF83">
        <property type="entry name" value="COMPLEMENT C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21309">
        <property type="entry name" value="C5_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="NTR" evidence="4">
        <location>
            <begin position="286"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013917739.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="48769" checksum="6235BA8B081D98BB" modified="2020-10-07" version="1">MVETTAYVLLSILLKGDHNYAKSVMKWLTEEQRFGGGFYSTQDTVIALECLTEYSILIKRLTLDMNVKASYKFQGDFHTYRLTHHNYIGRPVDASVKDNIDIHTGSSTGTAAVTLRSVYYVSSVSEDTCDFQLKIDVTLDEEKYYLSFNEVRLLVACAKYKPRRRTGQFSSPHAVIDVSLPNGLEANEEDLSLLSNSVDQLISKYEITPDGHVVMQVDSIPGNEFLCVGFRITEIYRVGVPSPGTFSVYDYHEPDKKCTIFYNAYGEESLVKLCEGSECRCMEAECGHMQPRLDTSITLDTRKEAACRDNIAYVYKVVIKSSKEEGSFVRYQAVLLEPYKLGVTFAEKNTEIILIKKSSCTDIQLNPKENYLIMGKEALKTGEGASAKYQYPLDDLTWIEWWPLPNTACDSCQTFRESLDEFVDDLFLSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRD8</accession>
    <name>A0A6I9XRD8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">LOW QUALITY PROTEIN: uncharacterized protein LOC106544554</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106544554</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013916326.1">
        <property type="nucleotide sequence ID" value="XM_014060851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRD8"/>
    <dbReference type="GeneID" id="106544554"/>
    <dbReference type="KEGG" id="tsr:106544554"/>
    <dbReference type="OrthoDB" id="62364at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17679">
        <property type="entry name" value="RUN_PLEKHM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051366">
        <property type="entry name" value="DEF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025258">
        <property type="entry name" value="RH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047326">
        <property type="entry name" value="RUN_PLEKHM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326:SF5">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY M MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13901">
        <property type="entry name" value="RH_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01175">
        <property type="entry name" value="DUF4206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RUN" evidence="11">
        <location>
            <begin position="323"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916326.1"/>
        </source>
    </evidence>
    <sequence length="908" mass="99894" checksum="C950E6EB4FF7177A" modified="2020-10-07" version="1">MPPIQVGADHQTIPTLGNNDFMPESSSLGSSPCQGTAMVSSPNAKIQNQQLPQMSASPTEGDPISGLVEDQGDRGGQASGGESRSSPGGAILAQEVLVRRPCQPLRSETVENSSGPNIPQPRGHQPPRSPVAKISRLALEGDLLRKQNISDKVICTIQVSRRPSTTRIYDATWAAFSSWCKDRGIVDFSASVPHTLDFLQEGLDKVLAPSTLRRQVVALSTILARSCSRSLRQYPQIKSFLKGAANISPPTVHRYLSWDLPFVLRALTGPPFEPLRTSSLRHLTIKTAFLVAITSTCRLIKKQLVNSIKALQKHYVTSDALVTSDDEDANTLCCVLEAVFVHGLNTKHIKAEVGAKGKKHGGRLPQPVFWTLLKSITHRNIISELENLVFINTDVGRCRAWLRLALNDGLMECYLKLLLHEKSQLSEYYHSPALLLDPEEVEFLLSYLQGLSSLAFDLSYKSAVLNEWTITPLSLSGLCPAWELLDPLVGLESQRKETLGFLSQSSGSDEIEAHPAILPVPKSQQADKLTASNLSISTTGSSQLSSSLDSDILLPANLTSTTSPAREKEPISNDSEFDMTAATADLDQDQDLQEVLAEFTRTQRKLESVGVHEANIVSRSSPPECPGPATSFCTAHLPRTVMAEASVDIMLTELMTNSQLPNLPKSGESVSSTSVQQTSMLATIIVTKEPVDVAQIHCNIKGGTKTWASEVNGSDGSLPGTDQLLSPVSPVVSPMILHFLSFPGCSRQIGFFFMKPKLCSFTGLYYCDNCHQDEESVIPSRLIHNWDLSRYPICCQALKFLAKRVQNQPLIDLKLVNETLYDHVEQMKQIYQNREQLKLLGDYLVLCRSGALKEISKRLDHWHYLLECPHKYSVADLRQVGDLEKRKCFNLEAGGNSVQENNTHFFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT54</accession>
    <name>A0A6I9XT54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106546805</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013919249.1">
        <property type="nucleotide sequence ID" value="XM_014063774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT54"/>
    <dbReference type="GeneID" id="106546805"/>
    <dbReference type="KEGG" id="tsr:106546805"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051491">
        <property type="term" value="P:positive regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030832">
        <property type="term" value="P:regulation of actin filament length"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000005">
        <property type="entry name" value="Myosin IIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000055">
        <property type="entry name" value="Putative microtubule-associated protein futsch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000008">
        <property type="entry name" value="Putative unconventional myosin-IXa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052409">
        <property type="entry name" value="Myosin-III_kinase_activity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46256">
        <property type="entry name" value="AGAP011099-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46256:SF1">
        <property type="entry name" value="MYOSIN-IIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11 17" id="KW-0518">Myosin</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Myosin motor" evidence="18">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013919249.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="43717" checksum="3F4F3519DB7D32B1" modified="2020-10-07" version="1">MDLLSKMVIGQPHFVRCIKPNDDREALKFSQERVLVQLRYTGILETVNIRQQGYSHRIHFDEFVKRYYYIAFKAHKNPSPSKENCIAILQKAKLDKWALGKTKVFLKYYHVEQLNLYLREVIGRVVIMQAYTKGWLGAKRYKKLKQKRTNSAITIQSAWRGYDSRKKFKDIKNKRIDAAIHIQAAFRGYLTRKNYRRWKNSIGYQADVESVGFVSSLRYEGNGEKNSSFPEKQCFLEKQTSKILAETSASLAILRHSDQVKDLQPFCYTRLAADHQLSCPQRPLESQENCLEQKQKPARRRCQQPKVLNSPEDNIYYNQLNGTLGYQGSKRKPRKLGQIKFLDWEDEYYKSLSVVDSITEEDNTFQPLTPFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTI9</accession>
    <name>A0A6I9XTI9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Retinal dehydrogenase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ALDH1A1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917332.1">
        <property type="nucleotide sequence ID" value="XM_014061857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTI9"/>
    <dbReference type="GeneID" id="106545343"/>
    <dbReference type="KEGG" id="tsr:106545343"/>
    <dbReference type="CTD" id="216"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07141">
        <property type="entry name" value="ALDH_F1AB_F2_RALDH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000050">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917332.1"/>
        </source>
    </evidence>
    <sequence length="499" mass="54274" checksum="801C350814B51928" modified="2020-10-07" version="1">MSAAKTLPAPLSDLKIQYTKIFINNEWHNSASGKKFPVYNPATGEKICEVEEGDKEDVDKAVKAARKAFEFGSSWRTADASERGRLLSKLADLIERDSLLLATMESLNGGKVFSHAYLMDLGGCINTLRYFAGWADKIQGRTIPMDGNYFTFTRHEPVGVCGQIIPWNFPLVMFIWKIAPALCCGNTVVVKPAEQTPLTALHMGALIKEAGFPPGVVNIVPGYGPTAGAAISNHMDVDKVSFTGSTEVGQMIKEAAGKSNLKRVTLELGGKSPNIIFADADLDSAVEFAHIGVFYHQGQCCIAGSRIFVEEKIYDEFVRRSVERAKKNILGNPLSPGVSQGPQIDKEQYDKILGLIESGKKEGAKLECGGGPGGDKGFFIQPTVFSDVTDDMRIAKEEIFGPVQQIMKFKTIEEVIKRANNTRYGLAAAVFTKDIDKALTFAAALQAGTVWVNCYSAVSAQSPFGGFKMSGNGRELGEYGLHEYTEVKTVTIKIPQKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XU04</accession>
    <name>A0A6I9XU04_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sterile alpha motif domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SAMD5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts promiscuously (via SAM domain) with EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3 and EPHB4 (via SAM domain) (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908210.1">
        <property type="nucleotide sequence ID" value="XM_014052735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XU04"/>
    <dbReference type="GeneID" id="106538286"/>
    <dbReference type="KEGG" id="tsr:106538286"/>
    <dbReference type="CTD" id="389432"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09527">
        <property type="entry name" value="SAM_Samd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000055">
        <property type="entry name" value="Sterile alpha motif domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051725">
        <property type="entry name" value="SAM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301">
        <property type="entry name" value="SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301:SF8">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00065890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013908210.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18623" checksum="551E5AA48227177B" modified="2020-10-07" version="1">MCANVVYEWLKTLKLTQYAESFVDNGYDDLEVCKQIGEPDLDAIGVSLPQHRRRLHEAVRRLKEEDETTAGLYFTLEPQPPRRGSPGSDSYTGRLLEPREAKTWKDPPRQTPPLGHSWGASRKMELNSKELITYPKLRLKIMIRDKLIRDGITLSKPPYSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUF2</accession>
    <name>A0A6I9XUF2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PERQ amino acid-rich with GYF domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106538388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013908330.1">
        <property type="nucleotide sequence ID" value="XM_014052855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUF2"/>
    <dbReference type="GeneID" id="106538388"/>
    <dbReference type="KEGG" id="tsr:106538388"/>
    <dbReference type="OrthoDB" id="48509at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990635">
        <property type="term" value="C:proximal dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048009">
        <property type="term" value="P:insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF38">
        <property type="entry name" value="GRB10-INTERACTING GYF PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013908330.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="21791" checksum="A87834C137B68585" modified="2020-10-07" version="1">MAAETQTLNFGPEWLRALSSGGSVTSPPLSPALPKYKLADYRYGREEMLALFLKDSKIPSDLLDKEFLPILQEEPLLPLALVPFTEEEQRNFSLSVNSTAVLRLMGRGGGGGGTVVGAPRGRSSSRGRGRGRGESAFYQRSFDDVEGGFGRGGGREMHRSQSWEERGDRRFEKTGRKEPDLEAVNVLSIDANLHFSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUV6</accession>
    <name>A0A6I9XUV6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Iron-sulfur cluster assembly factor IBA57, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Iron-sulfur cluster assembly factor homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">IBA57</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GcvT family. CAF17/IBA57 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908460.1">
        <property type="nucleotide sequence ID" value="XM_014052985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUV6"/>
    <dbReference type="GeneID" id="106538491"/>
    <dbReference type="KEGG" id="tsr:106538491"/>
    <dbReference type="CTD" id="200205"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000015">
        <property type="entry name" value="IBA57, iron-sulfur cluster assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057460">
        <property type="entry name" value="CAF17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602:SF0">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25455">
        <property type="entry name" value="Beta-barrel_CAF17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CAF17 C-terminal" evidence="8">
        <location>
            <begin position="254"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013908460.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="36923" checksum="897F947888051C34" modified="2020-10-07" version="1">MLVRASAALRRGLPRNLRNSGGGGGDIRVTAVCFPLSHRALLRLQGPETLPFLQGLLTNDVTPFEEVGGAAPRALYAHALNVQGRCLYDVIAYRLHENKQEEPRVLLECDAGLLDSFRKHLKLYKIRRKLDISPCPDLSVWAVVPKEPSDSLQQAGKAMILTADPRVKIMGWRLIAHKDVNPLEIVPGGQLGELKDYHRHRYQQGIPEGVKDLPYGTALPLESNLVYLNGVSFTKGCYIGQELTARTHHMGVIRKRLLPVQLSPPMPFESLAEGTEIVAESGKSAGKFRAGENEFGIALLRLAATKGPLRLKISNDSQVMVTASIPTWWPQPANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVG5</accession>
    <name>A0A6I9XVG5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium-dependent multivitamin transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Solute carrier family 5 member 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">SLC5A6</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Sodium-dependent multivitamin transporter that mediates the electrogenic transport of pantothenate, biotin, lipoate and iodide. Functions as a Na(+)-coupled substrate symporter where the stoichiometry of Na(+):substrate is 2:1, creating an electrochemical Na(+) gradient used as driving force for substrate uptake. Required for biotin and pantothenate uptake in the intestine across the brush border membrane. Plays a role in the maintenance of intestinal mucosa integrity, by providing the gut mucosa with biotin. Contributes to the luminal uptake of biotin and pantothenate into the brain across the blood-brain barrier.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(R)-lipoate(out) + 2 Na(+)(out) = (R)-lipoate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73379"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-pantothenate(out) + 2 Na(+)(out) = (R)-pantothenate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73371"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>biotin(out) + 2 Na(+)(out) = biotin(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73375"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>iodide(out) + 2 Na(+)(out) = iodide(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71207"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with PDZD11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918087.1">
        <property type="nucleotide sequence ID" value="XM_014062612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVG5"/>
    <dbReference type="GeneID" id="106545898"/>
    <dbReference type="KEGG" id="tsr:106545898"/>
    <dbReference type="CTD" id="8884"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090482">
        <property type="term" value="F:vitamin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098660">
        <property type="term" value="P:inorganic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015887">
        <property type="term" value="P:pantothenate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000011">
        <property type="entry name" value="sodium-dependent multivitamin transporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF2">
        <property type="entry name" value="SODIUM-DEPENDENT MULTIVITAMIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="402"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013918087.1"/>
        </source>
    </evidence>
    <sequence length="624" mass="67942" checksum="A97EE77F1EEF451F" modified="2020-10-07" version="1">MKFTVADYAIFVLLLVFSLGIGLFYALSGGKQRTVQEFLLANRSMTFLPLAFSLLATFQSAVAILGVPAEIYRFGTEYWFLGCSYFLGLLIPAHIFIPIFYRLRLTSTYEYLELRFNKVVRLCGTATFIFQMVIYMGVVLYAPALALNAVTNFDIWASVLTIGLVCTLYTALGGLKAVIWTDVFQTFVMFAGQVAVIVVGTLKVGGIGRVWKLAADKGMISGIDLNPDPFERHTFWTLAFGGVFMMLALYGVNQAQVQRYLSSRSERQAVLSCYAVFPCQQIVLCLSCLTGLVMFAYYQDHQLTQEQLNGSSDQMVLFFVMDVLQDVPGLPGLFVACLFSGSLSTISSAFNSLATVTMEDLIRPYVTNIPERRATLYSKLLALGYGLLCLGMAYISSMMGPVLQAAISIFGMVGGPLLGLFCLGMFFPCANSVGAVVGLIAGLAMAFWVGIGSMVINYQSVSPDPTVLATDGNLTTAVMTTLLNSTTAPERLTGLRKFYNLSYMWYSAHNSTTVIVVGLLVSLLTGPTRGKDVNPKTIFLVLPRLFCCLPAKYHEMLRCGVRDPAEDASCSEPTSSGAEKINNGMANGLSKGLPSGVNTEEVEASEGFSRVDGTHTYVLQETTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW27</accession>
    <name>A0A6I9XW27_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tubulin polyglutamylase TTLL7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TTLL7</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914858.1">
        <property type="nucleotide sequence ID" value="XM_014059383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW27"/>
    <dbReference type="GeneID" id="106543376"/>
    <dbReference type="KEGG" id="tsr:106543376"/>
    <dbReference type="CTD" id="79739"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000009">
        <property type="entry name" value="tubulin polyglutamylase TTLL5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="379"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013914858.1"/>
        </source>
    </evidence>
    <sequence length="537" mass="62713" checksum="EC35DF08BCD3451E" modified="2020-10-07" version="1">MPSLPNEGDNQGTTSLSLSSSLSFQSSIRRKVREKKKHATITANIAGTKYEIVRVAIEEMGFAKTRDDDETANLIWSDCAVQQEKIADLQNYQRINHFPGMGEICRKDFLARNMTKMIKSQPQEYSFIPRTWIFPAEFTQFQNYVKELKRKRRQKTFIVKPANGAMGHGISLVRNAEKLHSQDHLIVQEYLDKPFLMEGYKFDLRVYILVTSCDPLKIFLYHDGLVRMGTEKYHPPNESNMSQLYMHLTNYSVNKHNEHFERDETEDKGSKRSIKWFTEFLQTNHLDVSKFWNDISELVVKTLIVAEPHVLHAYRMCRPGQSPGSDSVCFEVLGFDILLDKKLKPWLLEINRAPSFGTDQKIDYDVKKGVLLNALQLLNIRTSDKRKNLAKQKAEAQKRLYGQGSMKRLSPISSDWEKQRRSLERRREELKERLAQVRRQISKEEHENRHMGNYRRIYPPDDKFLLEKYEGLLATAFQTFLAGRAASLQREMNNPLKRMKVLIVFSASSFESNRCPLRYSFNFGKKWKLHRTKSGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XX29</accession>
    <name>A0A6I9XX29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Short-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="4">1.3.8.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Butyryl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ACADS</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57389"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43457"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013920769.1">
        <property type="nucleotide sequence ID" value="XM_014065294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XX29"/>
    <dbReference type="GeneID" id="106547997"/>
    <dbReference type="KEGG" id="tsr:106547997"/>
    <dbReference type="CTD" id="35"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016937">
        <property type="term" value="F:short-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="18">
        <location>
            <begin position="28"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="143"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="16">
        <location>
            <begin position="250"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013920769.1"/>
        </source>
    </evidence>
    <sequence length="404" mass="43242" checksum="52B2A5425DF9964C" modified="2020-10-07" version="1">MAALVASAGLRGVLGARGARLLHAGLPETHRMLRETCRDFAAKELAPLAARLDRDHRFPAEQIKKMGALGLLAMAVPEKYRGAGLDCLAYTVATEEISRGCASTGVVMSVNNSLYLGPVLKFGYEEQREQWIPPFTSGEKVGCFALSEPGNGSDAGAASTVAQLVGDEWVLNGTKAWITNAWEASATVVFATTDRALKHKGISAFLVPMPCPGLSLGKKEDKLGIRASSTANLIFEDCRIPKENLLGTQGMGFKIAMQTLDSGRIGIASQALGIAQAALDCAVDYAEKRLAFGHPITKLQAIQFKLADMAVALEAARLLTWRAAALSDKGKPYTKEAAMAKLSASEAATFISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIAGQLLKAYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXS4</accession>
    <name>A0A6I9XXS4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel subfamily G member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106543837</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915403.1">
        <property type="nucleotide sequence ID" value="XM_014059928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXS4"/>
    <dbReference type="GeneID" id="106543837"/>
    <dbReference type="KEGG" id="tsr:106543837"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18421">
        <property type="entry name" value="BTB_POZ_KCNG1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000024">
        <property type="entry name" value="Potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000019">
        <property type="entry name" value="Potassium voltage-gated channel, subfamily G, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003969">
        <property type="entry name" value="K_chnl_volt-dep_Kv6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF88">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY G MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01492">
        <property type="entry name" value="KV6CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="63"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013915403.1"/>
        </source>
    </evidence>
    <sequence length="510" mass="58310" checksum="6244A7BF673FC0ED" modified="2020-10-07" version="1">MTLLPGENSDYDYSALSCASDASFNHPLFAERETLKGVFYQRAKRLHPGKDLYESVRPEERKHYIIINVGGFKYLLPWTTLDEFPMTRLGQLKFCNNFDDILNICDDYDVTCNEFFFDRNPGAFRTILTFLRAGKLRLLREVCALSFQEELLYWGIEEDSLEWCCKRRYLQKMEEFAEMNEIEDEFLDCENPSEATGTETKGSLCMKKLQDMVEKPQSGIPGKVFACLSVLFVTVTAVNLSISTMPGLREEEERGECSQMCYNIFIVESVCVGWFSLEFLLRFIQAPSKFIFLRHPLTLIDIVAILPYYITLLLDSASVSSNKKPSSGNIYLDKVGLVLRILRALRILYVMRLARHSLGLQTLGLTARRCTREFGLLLLFLCVAIALFAPLLYVIENEMADSQEFTSIPACYWWAVITMTTVGYGDMVPRSIPGQVVALSSILSGILLMAFPVTSIFHTFSRSYIELKQEQERIMYRRAQFLMKTKSQLSNASQGSDVFPNMPSETRDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYB3</accession>
    <name>A0A6I9XYB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106539237</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013909465.1">
        <property type="nucleotide sequence ID" value="XM_014053990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYB3"/>
    <dbReference type="GeneID" id="106539237"/>
    <dbReference type="KEGG" id="tsr:106539237"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF51">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="311"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909465.1"/>
        </source>
    </evidence>
    <sequence length="605" mass="69356" checksum="EC2C831C7C92F022" modified="2020-10-07" version="1">MLGFLKQPIVFSVEINVNLVVLTVVGLVSRLWALSYPRAVVFDEVYYGQFLSLYMKRIFFIDDSGPPFGHMLFALGGYLGGFDGNFLWNRIGAEYSSNVPIWALRLLPAIAGGLCVPLAYQILVELHFTHFVALGAAVLILLENSLITQSRLMLLESILIFFILLAVLSYLKFHNSHKQSPFSARWWIWLLLTGVSCSCAVGVKYMGLFTYLLILSLAGIHSWQLVGDRSLSNLGNETSAKKTNHYREHQGLHSPTMSSKYSFPLLSLLCRHDVAAPLSPHSQEVSCYIDYNISMPAQNLWRVEIVNRDSDNEIWKTILSEVRLVHVNTSAVLKLSGASLPEWGYRQLEVIGEKISKGYHQSMLWNVEEHRYGKSHEQKEREVELHLPTQINISQNLTFVAKFTELQWKILTLKNEDIEHCKYSSSPLDWITLETNIAYWFHSSSGAQIHLLGNLVNWTSANVATIVYVILFLGYLLRRRRNIRDIPEETWQQWLLAGGICVGGWAMNYLPFFLMEKNLFLYHYLPAVTFQIPLIPVVLQHTSDFLCRSRLYKSMHSALVAAWISSVYLTYHTFRPLTYGEPALSKSELQALRWKDSWDILIRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYC9</accession>
    <name>A0A6I9XYC9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RYK</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013909485.1">
        <property type="nucleotide sequence ID" value="XM_014054010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYC9"/>
    <dbReference type="GeneID" id="106539250"/>
    <dbReference type="KEGG" id="tsr:106539250"/>
    <dbReference type="CTD" id="6259"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05043">
        <property type="entry name" value="PTK_Ryk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000165">
        <property type="entry name" value="Tyrosine-protein kinase RYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000218">
        <property type="entry name" value="Tyrosine-protein kinase RYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF349">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="103"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909485.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="43177" checksum="B6A1D950E21A24C2" modified="2020-10-07" version="1">MLYPQKTEEDFFFKLQVNVLETIMYFVFVFQSSISNSSSSQGLSQPSTQTTQYLRADTPNNATPITSSSGYPTLRIEKNDLKSVTLMEAKAKVKDIAISRERITLKDVLQEGTFGRIFHGILIEEKDPSKEKQIFVKTVKDQASEIQITMMLTESSKLRGLHHRNLLPITHACIEEGEKPMVLLPYMNWGNLKLFLRQCKLVEANNPQAISQQDLVHMAIQIACGMSYLARREVIHKDLAARNCVIDDALQVKITDNALSRDLFPMDYHCLGDNENRPVRWMALESLVNNEFSGASDVWSFGVTLWELMTLGQTPYVDIDPFEMAAYLKDGYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHAALGAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYT4</accession>
    <name>A0A6I9XYT4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BTB/POZ domain-containing protein 7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BTBD7</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915743.1">
        <property type="nucleotide sequence ID" value="XM_014060268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYT4"/>
    <dbReference type="GeneID" id="106544114"/>
    <dbReference type="KEGG" id="tsr:106544114"/>
    <dbReference type="CTD" id="55727"/>
    <dbReference type="OrthoDB" id="2347980at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061138">
        <property type="term" value="P:morphogenesis of a branching epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18489">
        <property type="entry name" value="BACK_BTBD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042345">
        <property type="entry name" value="Btbd7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047936">
        <property type="entry name" value="BTBD7_BACK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16064">
        <property type="entry name" value="BTB POZ DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16064:SF3">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BACK" evidence="2">
        <location>
            <begin position="39"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915743.1"/>
        </source>
    </evidence>
    <sequence length="752" mass="84934" checksum="13D2734BF4762A1B" modified="2020-10-07" version="1">MTKAEEAMELYHIALFLDFSMLAQGCEDIIAESISLETLTAVLKWSSQPYGSKWVHRQALHFLCEEFSQVMTSDVFYELCKDHLQTAIQSDYLQASEQDILKYLVKWGEHQLIKRIADREPNLLSGTAHSVNKRGVKRRDLDIEELKEILSPLLPFVRIEHILPMNSEVLTDSMKRGLISTPPSDMLPTAEGGKSNVWLRQKNAGIYVRPRLFSPYVEEAKSLLDEMMVEQTDLVRLRMVRMSNVPDTLYMVNNAVPQCCHMINHQQMSSNQSSPPSVVANEIPVPRLSVVKEMVRRLQELRHTEQVQRAYALNCGEGATVSYEIQIRVLREFGLSDSAAELLQNPHKFFPDEHFGDESPLLTLRQSGRCRVNSTPTAETVFTDLDSFVAFHPPLPPPPPPYHPPATPVHSQFKVGWKQRTQSQHPSRSFSYPCNHSLFHARTAPKPVLPNYFSSVKGTPPDCTNTTGLGRQTVAAAAAVVAAEKQVGIQPVLNELMPDIAMGVSTISLKDRRLPELTVDTEPVLEVTPGPPQHISCIQTRHIHSSRKKHSAEQKLDPRECQQEYPDFYDFSNAACRPSTPSLTRHTPSPSQGIYFSPDLYSHNKASPSGLKTTQLPNHISPKKLEDSRRDYALSQEGHSHRQKNETIHLNVLEQPPQRLDLALAAQENPGNLSIHARGRTKMETDLTYGLTSSRPSLPSYREEIRDEPSSRRLVDTEPLESEVQRNVDLEREEVVSRGRKSPDFLYKKSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y149</accession>
    <name>A0A6I9Y149_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C20orf194-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106547879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920617.1">
        <property type="nucleotide sequence ID" value="XM_014065142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y149"/>
    <dbReference type="GeneID" id="106547879"/>
    <dbReference type="KEGG" id="tsr:106547879"/>
    <dbReference type="OrthoDB" id="72033at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056414">
        <property type="entry name" value="DAAF9_CobW_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040342">
        <property type="entry name" value="DNAAF9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33664:SF1">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33664">
        <property type="entry name" value="RCG26366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23319">
        <property type="entry name" value="CobW_C_DAAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAAF9 CobW C-like" evidence="1">
        <location>
            <begin position="28"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920617.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="11547" checksum="15B3ACDB8C62B816" modified="2020-10-07" version="1">MLATRYLMYPGWFDGKFRAGSVFPPMVQICVWFNRPLEKMRFAAKCKALKSSNKSSPFSGNIYHIFGKVKFSGNCSASTLPPFLNYFILNPCRCHLRTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1F9</accession>
    <name>A0A6I9Y1F9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tripartite motif-containing protein 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106547983</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Neurotoxin that produces dose-dependent hypolocomotion and hyperalgesia in mice. May directly act on the central nervous system, as it is 6500-fold more potent when administered intracerebroventricularly than intraperitoneal.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ohanin/vespryn family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920742.1">
        <property type="nucleotide sequence ID" value="XM_014065267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1F9"/>
    <dbReference type="GeneID" id="106547983"/>
    <dbReference type="KEGG" id="tsr:106547983"/>
    <dbReference type="OrthoDB" id="654191at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19762">
        <property type="entry name" value="Bbox2_TRIM7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16594">
        <property type="entry name" value="RING-HC_TRIM7-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0528">Neurotoxin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0800">Toxin</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="330"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013920742.1"/>
        </source>
    </evidence>
    <sequence length="518" mass="59290" checksum="538D4A2FA90DE78C" modified="2020-10-07" version="1">MTLQHQLASDRENPALGFASSIRALYAAAKAGREEEEEGGAMAAVLLPGNLQDEATCSVCLEYFKDPVSIQCGHNFCRACITKSWKSLEVDFPCPQCRELFKEKSFRPNRQLANMSEIISQFILHGGRGLEEDRICEKHKEALKLYCKDDQKTICVVCDRSRDHRPHAVVPVEEAAQEYKEQIQTRLDFLKKERQELLEFKTQDDKKSQELLKTIETERQKVLSEFEGLRQFLHDQEQVLLGQLDKMEKGIVKRQNENITELSKEISLLNKFIADLEDKSQESLLDFLKDVTAIIARSDNVKCQQPIPVSCDMKGHVSNFSLKTVALKGALKKFRDHLRDELGKGEKEDLLLDLETANHLLLISPDLKTVRMGCRKQDLSYSPKRFDTNSRVLATEGFKSGRHYWEVEVGSADGWAFGVAKESVRRKGLTQFCPEEGIWALQQNGGQYWAVTTPQRTPLFLNEKLIKVRVYLDYEGEEVSFYNADTMQHIFTFNIAFTEKMFPLFSVCSTITYIKLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y452</accession>
    <name>A0A6I9Y452_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="11">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PLCD4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013918518.1">
        <property type="nucleotide sequence ID" value="XM_014063043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y452"/>
    <dbReference type="GeneID" id="106546230"/>
    <dbReference type="KEGG" id="tsr:106546230"/>
    <dbReference type="CTD" id="84812"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000058">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF31">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE DELTA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="14">
        <location>
            <begin position="149"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="265"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="103"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013918518.1"/>
        </source>
    </evidence>
    <sequence length="419" mass="47282" checksum="EF2D3CC94A97F391" modified="2020-10-07" version="1">MLNEIIGEEVSVITTCVLTKSKNSLEMGSQKIKVSDYPIILSIENHCSIEQQDIMAQLLKGVLGERLLINTIDDLVPVQLPSPEELKGKIILKGKKIGYLEDSLNEHPDKMPEREEVIEEEVNETEEEALRSEDKRKEKKGKHSLSKELSDCIIYCKSVPFCSFQHSCSHYKSYEMSSITESKARKLIREAGNDFVRHNTWQLTRIFPSGLRTDSSNFNPQAMWNVGCQLVALNFQTAGTEMDLYDGLFSQNGHCGYVLKPSFMRDKETAFSPEDPQSRGEGNPLSLMVKIISGQQLPKIPNSKEGSIVDPFVRVEIHGIPSDSAKQETKYIDNNGFNPQWGESFRFYVWVPELALLRFVVEDYDKASRNDFVGQYTLPLTSVKAGYRHIHLLSKDGTGISPASLFVHIQIGELATEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y487</accession>
    <name>A0A6I9Y487_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Testis-expressed sequence 26 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TEX26</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918648.1">
        <property type="nucleotide sequence ID" value="XM_014063173.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106546328"/>
    <dbReference type="KEGG" id="tsr:106546328"/>
    <dbReference type="CTD" id="122046"/>
    <dbReference type="OrthoDB" id="5984625at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043460">
        <property type="entry name" value="MEDAG/TEX26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33769:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33769">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 26 ISOFORM X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013918648.1"/>
        </source>
    </evidence>
    <sequence length="270" mass="31086" checksum="75C94DC7F694179E" modified="2020-10-07" version="1">MKHDFVFKTATGPEADCARAGTGHVIPFQVDEPTAKSIYRDEFCWKPYSKAEPITNGFMTVKDRNNPQPGDRFLIWQLPREETALPTDSFSPWTKPFSKQDLEAVLKKQYKTVYTRDYLGMQPGTPRDDDVSPPDWKTLIPKPPDTEVRRHYQPQVRAPELMDFTWKYGCNAKRKIPIKGAVPSMTIAQIWNHEHSKHLSTYQKDFGNDYLDILSVLNSVDPEEIKEYVERAPKPEKIILQNFLDRVSGDKILQRSSSSKKSGENPIRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4J0</accession>
    <name>A0A6I9Y4J0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Squalene synthase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106546567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918938.1">
        <property type="nucleotide sequence ID" value="XM_014063463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4J0"/>
    <dbReference type="GeneID" id="106546567"/>
    <dbReference type="KEGG" id="tsr:106546567"/>
    <dbReference type="OrthoDB" id="431150at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045338">
        <property type="term" value="P:farnesyl diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044844">
        <property type="entry name" value="Trans_IPPS_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626">
        <property type="entry name" value="FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626:SF2">
        <property type="entry name" value="SQUALENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013918938.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="15519" checksum="AD84AF4012E45B4C" modified="2020-10-07" version="1">MPLNNAVVLKCIRLKCELMLFIPYNHAVCIFYLVLRALDTVEDDMTISLEAKVPMLHNFHSYLYQPDWKFMDSREKDRQVLEDFPTISLEFRNLAKVYQDVITDICHKMGLGMAEFLEKKVNSNKEWDKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5Q0</accession>
    <name>A0A6I9Y5Q0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">B-cadherin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106542204</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913340.1">
        <property type="nucleotide sequence ID" value="XM_014057865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5Q0"/>
    <dbReference type="GeneID" id="106542204"/>
    <dbReference type="KEGG" id="tsr:106542204"/>
    <dbReference type="OrthoDB" id="6079678at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000095">
        <property type="entry name" value="Cadherin 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000022">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000031">
        <property type="entry name" value="Cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF446">
        <property type="entry name" value="CADHERIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="95"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="207"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="313"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913340.1"/>
        </source>
    </evidence>
    <sequence length="599" mass="65092" checksum="6F7259EDF3042637" modified="2020-10-07" version="1">MQVTATDADDAIETYNGVVAYSILSQAPQEPHLQMFAINRATGTISVIASGLDRERVGEYTLILQAADLDGLGLTTTASAVIEITDANDNVPEFTESMYSAEVAENMEGVEVVRLAVTDRDERHSPAWHAVYSIVQGNGGELFSISTDPKTNEGILTTAKALDFETGQQFVLQVAVANEVPFAVKLPTSTATVTISVTDANEAPVFAPPALKVKVSEDIPVGQKIATYAAQDPDTAQSQKIRYLLESDPAQWLDVHPETGIITAKAPLDRESPLVQNDTYVAIVLAVDDGSPPATGTGTLLLTLLDVNDHGPEPDRRKFTVCNHNPSPQLLRISDKDLPPHTSPFQAELVHNSEENWSVEMDSEGETAILKLLKPLKRETYDVYLRLLDQQGKSHLTVLRATVCECEGEVDECLEGPMPIVGAPIILAVLGAVLALLILLLLLLLFARRRKVAKEPLLLPEDDTRDNIFYYGEEGGGEEDQDYDLSQLHRGLDPRPEVLLRNDVVPAPLPAPQYRPRPANPDEIGNFICENLKAADLDPTAPPYDSLLVFDYEGGGSEGGSLSSLNSSASDQDQDYDYLHEWGSRFQKLADLYGGGVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5Y7</accession>
    <name>A0A6I9Y5Y7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Deoxyribodipyrimidine photo-lyase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106542379</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913565.1">
        <property type="nucleotide sequence ID" value="XM_014058090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5Y7"/>
    <dbReference type="GeneID" id="106542379"/>
    <dbReference type="KEGG" id="tsr:106542379"/>
    <dbReference type="OrthoDB" id="496749at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000719">
        <property type="term" value="P:photoreactive repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.80:FF:000004">
        <property type="entry name" value="Deoxyribodipyrimidine photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000110">
        <property type="entry name" value="Deoxyribodipyrimidine photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032673">
        <property type="entry name" value="DNA_photolyase_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052219">
        <property type="entry name" value="Photolyase_Class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10211:SF0">
        <property type="entry name" value="DEOXYRIBODIPYRIMIDINE PHOTO-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10211">
        <property type="entry name" value="DEOXYRIBODIPYRIMIDINE PHOTOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01083">
        <property type="entry name" value="DNA_PHOTOLYASES_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="3">
        <location>
            <begin position="87"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913565.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="47877" checksum="7F12CE481A03169E" modified="2020-10-07" version="1">MSPKKRKPGPATSTSDSSEGGRKPDDGGTEAFAAKKKLKLDDSRTQSLAESVKEARLSAGASVVEFPYNKRRVRLISPAPDLKEGARGILYWMSRDQRVQDNWAFLYAQRLALKQRLPLHVCFCLVPKFLDATIRHFGFMLRGLEEVAEECQLLDIPFHLLVGFAKDTLPRFVTQHVIGGVVTDFSPLRVPMQWVRDIQERLPSDVPLAQVDAHNIVPCWVASEKQEYGARTIRRKIHDQLPKFLTEFPPVVKHPFPAGRPAEPINWEACYSSLEVDRSVKEVEWATPGSSFGLKVLEEFVKDRLKSFGSDRNNPNRPALSNLSPWFHFGQVAVQRAILEVRKHRARYKESVEGFIEEAVVRRELADNFCFYNPNYDKVEGAYDWAKTTLKLHAKDKRSHLQQASDLGPLPPAVGYSQHPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6I8</accession>
    <name>A0A6I9Y6I8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Catenin alpha-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CTNNA1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914065.1">
        <property type="nucleotide sequence ID" value="XM_014058590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6I8"/>
    <dbReference type="GeneID" id="106542768"/>
    <dbReference type="KEGG" id="tsr:106542768"/>
    <dbReference type="CTD" id="1495"/>
    <dbReference type="OrthoDB" id="6376697at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000006">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000007">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000008">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000011">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000012">
        <property type="entry name" value="Catenin alpha-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001033">
        <property type="entry name" value="Alpha_catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000633">
        <property type="entry name" value="Vinculin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914">
        <property type="entry name" value="ALPHA CATENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914:SF24">
        <property type="entry name" value="CATENIN ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00805">
        <property type="entry name" value="ALPHACATENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00663">
        <property type="entry name" value="VINCULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="864"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="864"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="880"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013914065.1"/>
        </source>
    </evidence>
    <sequence length="905" mass="100261" checksum="1FE6A52658E4E586" modified="2020-10-07" version="1">MTTINTGNVNFKWDPKSLEIRTLAVERLLEPLVTQVTTLVNTSNKGPSNKKRGRSKKAHVLAASVEQATENFLEKGDKIAKESQFLKEELVAAVEDVRKQGDLMKSASGEFADDPCSSVKRGNMVRAARALLSAVTRLLILADMADVYRLLVQLKVVEEGILKLRNAGTEQDLGIQYKALKAEVDKLNIMAGKRQQELKDIGHRDQMAAARGILQKNIPILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASEDSVQHPSGGGELADALNNFDKQIIVDPLKFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYAQVFREHANKLIEVANLACSISNNEEGVKLVHMSASQLEVLCPQVINAALALAAKPQSKLAQENMDLFKELWEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEMDNYEPGVYTEKVLDATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIHGHSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSSNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y784</accession>
    <name>A0A6I9Y784_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Carbohydrate-responsive element-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MLXIPL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013914730.1">
        <property type="nucleotide sequence ID" value="XM_014059255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y784"/>
    <dbReference type="GeneID" id="106543278"/>
    <dbReference type="KEGG" id="tsr:106543278"/>
    <dbReference type="CTD" id="51085"/>
    <dbReference type="OrthoDB" id="6022628at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000028">
        <property type="entry name" value="MLX interacting protein like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF14">
        <property type="entry name" value="CARBOHYDRATE-RESPONSIVE ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="29"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914730.1"/>
        </source>
    </evidence>
    <sequence length="233" mass="26198" checksum="0290001E62182261" modified="2020-10-07" version="1">QGPPVFLEGLVSSPLYASQTRAEPSKTESRRIIHISAEQKRRFNIKLGFDTLHGLVSTLSAQPSLKISKATTLQKTAEYICKLQQERATLQDETQRLRDQIEELNVSINVCQQQLPDTGVPITRQRFDQMRHMFDEYVRLCTLQNWKFWVFSIIMRPLFESFNGMVSTSSLESLSQTSLAWLEQYCSLPALRPTVLSSLRQLSVSTSILTNPSGIPEQATQAVTGTSTIDGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7E0</accession>
    <name>A0A6I9Y7E0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Swi5-dependent recombination DNA repair protein 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Meiosis protein 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 14 15 16 17 18 19" type="primary">SFR1</name>
    </gene>
    <organism evidence="12 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SFR1/MEI5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915007.1">
        <property type="nucleotide sequence ID" value="XM_014059532.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915008.1">
        <property type="nucleotide sequence ID" value="XM_014059533.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915009.1">
        <property type="nucleotide sequence ID" value="XM_014059534.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915010.1">
        <property type="nucleotide sequence ID" value="XM_014059535.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915011.1">
        <property type="nucleotide sequence ID" value="XM_014059536.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915012.1">
        <property type="nucleotide sequence ID" value="XM_014059537.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915013.1">
        <property type="nucleotide sequence ID" value="XM_014059538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7E0"/>
    <dbReference type="GeneID" id="106543501"/>
    <dbReference type="KEGG" id="tsr:106543501"/>
    <dbReference type="CTD" id="119392"/>
    <dbReference type="OrthoDB" id="10051617at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032798">
        <property type="term" value="C:Swi5-Sfr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042429">
        <property type="entry name" value="SFR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018468">
        <property type="entry name" value="SFR1/Mei5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28643">
        <property type="entry name" value="SWI5-DEPENDENT RECOMBINATION DNA REPAIR PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28643:SF1">
        <property type="entry name" value="SWI5-DEPENDENT RECOMBINATION DNA REPAIR PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10376">
        <property type="entry name" value="Mei5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013915007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013915008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013915010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013915011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013915012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013915013.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="26511" checksum="4E14D4D678AFCC47" modified="2020-10-07" version="1">MGTPILNKPKSSSSTPENCENSVQSNRLGKQLMSATLRERLKKTRRSFDSNFIVAKQLKIDDENNSNRKEKALSKEEPCFVSHAEDKCLKKVPDEIMCSESSAENRSLSEYNEYIKATHTSSEINSLGTNCKQWELLEEKMTIMKQLQEKEDILRRLKLVKLYRTKNNPEELQFLISKWRKSSQAILYELQSALPTDNKKLSLTQLIDSFGLDDKLLHYIRTNEDFTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7T6</accession>
    <name>A0A6I9Y7T6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Guanylate cyclase</fullName>
            <ecNumber evidence="2 14">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106544109</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="2 14"/>
    <dbReference type="RefSeq" id="XP_013915740.1">
        <property type="nucleotide sequence ID" value="XM_014060265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7T6"/>
    <dbReference type="GeneID" id="106544109"/>
    <dbReference type="KEGG" id="tsr:106544109"/>
    <dbReference type="OrthoDB" id="1890790at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000545">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000004">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001170">
        <property type="entry name" value="ANPR/GUC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF500">
        <property type="entry name" value="GUANYLATE CYCLASE 2G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00255">
        <property type="entry name" value="NATPEPTIDER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="488"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="498"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="912"/>
            <end position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013915740.1"/>
        </source>
    </evidence>
    <sequence length="1122" mass="128171" checksum="C625389B9FCB4671" modified="2020-10-07" version="1">MEVCKGVLIFLAMMQQSLNKYSQYSFSIYYGSIIFVLLTIYLSTCNTILGTERSKLVVAFQAPCNISHPLSVQRLTSALETAINKVNADPSYLYYYKLEYVYGDCSCDAKKSLQAFINQVQNENISALFGPVCSEAAEVTGLLASQWNIPMFGFVGQTTKLRNSFFYDTYINLVSPVYLIGEVLHKTLQYFGWKYVGLFGSSYDAFTWGETEELWTSIENQLKFNITITVKVRYNTKNQSLHEEKLNYISSVARIIVLICNPLDAKYILLQAKELGMTNGPYVFFVLQRFEAYFLEETWNHENTSILDAYQSVFLIALRSYREYKNYSYFMKELYDKLKERQPSYNLFSLQEEVITLNSYAAYLHDAVLLYASTVKEMLEDKKDFLDGRALINTLKGYNKTPLYGITGPVYIDELGERKMDYSVYDLQESGNSTHFIPVLNFDTYRRTLSPTIEVNYIGWSNGEIPKDKPVCGFHNEHCKSQTTKATALIIMIILLVITILGTIGATIFMKIYKGNFQKQISDSGWRINYEDISFLNNNKEKCDLPQASTSEPEQTENVGAHLFISSSNYSSLKDKQGKEDFFTTVGLYQGILVAVKFIDNQTDALVWKPSVFQEIQMMRELRHENLVIFFGICPEVPNICIVMHYCKKGSLKDVLLHSAIELDWIFKISFAYDIVNGMFFIHNSPLKSHGNLKPTNCLVDSRMQIKLCDFGLWELKYRRKSRIIEEETNYADLFWTAPELLRMEEYPFHGTQKGDIYSFAIIMRELIYNHEDGPFQDQNKSAEEIIKRIKDSKSSVPLRPSISIDKCNCKVTELLKACWNENPEKRPIFSEIKTALRDASPDSHFSILDNMVNKLEKYANHLEEVVEARTTLLLAEKKKTETLLSTMLPSFIGEQLVAGRSVEPESFESVSIFFSDIVGFTKLCSFSSPLQVVTLLNDLYSLFDNIIQIYDVYKVETIGDAYMVASGLPIPNGEKHVEEIATMSLHFLSAIISFKIRHLPNEKLRVRIGIHTGPVVAGVVGITRPRYCLFGDSVNTASRMESTSLPLQIHVSNTTANALQRIGGYNLEERGTIRIKGKGQQRTFWLKGKAGFDMPLPDFNSKFQPQLKGNEDAEYVAKDFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7Y5</accession>
    <name>A0A6I9Y7Y5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermatogenesis-associated protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SPATA6</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915985.1">
        <property type="nucleotide sequence ID" value="XM_014060510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7Y5"/>
    <dbReference type="GeneID" id="106544273"/>
    <dbReference type="KEGG" id="tsr:106544273"/>
    <dbReference type="CTD" id="54558"/>
    <dbReference type="OrthoDB" id="5963614at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120212">
        <property type="term" value="C:sperm head-tail coupling apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032027">
        <property type="term" value="F:myosin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042769">
        <property type="entry name" value="SPATA6_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032732">
        <property type="entry name" value="SPATA6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435:SF3">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6 SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14909">
        <property type="entry name" value="SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 6 N-terminal" evidence="4">
        <location>
            <begin position="11"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013915985.1"/>
        </source>
    </evidence>
    <sequence length="487" mass="56177" checksum="A342F82173511320" modified="2020-10-07" version="1">MAPCKGFVCVLELHIRTVTCPGVALKSREDVYVSACIFGQHKKTSCVRAIFPLMFNEKLMFEKVYTDVVDPGELVELFETDTSVVQLIQTVPPVGEILSRYEDNTRDFLFPAPKFTHQPRNSVREVLMKKAYEFTGIAPKLKFHTTCVISESLLSSAKVQKQKQDNLDGLFHSSTEGIRQIKSSKKNTASSERNRPCLATKSYEQPTIASLSRSPSPYTKRRMCELLQISQRLAHLDLGPFDFRKETDRPPFVIRHVEELTPSPDVHTWCRSKDNNKEGAPYDPSLLGSYKPKNKIIGPHHEKYFDYFPDAYEKHFLTKMSSRQIYSNRLLMHSAPASLPKFFSTPMLDRSSLRERYYTGWSTPINGDEIHKRVKNILRTHSARQKLTFDETSLSKEESAKTRDSTRTFDFRKRDFSHTDSLNSNFQNSSFSQPNVMVHLDNDEYWTSQVSEYKNKPHRAIFEESLGKIYRNMYRKASSSLSKNKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9B3</accession>
    <name>A0A6I9Y9B3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sesquipedalian</fullName>
            <shortName evidence="9">Ses</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PH domain-containing endocytic trafficking adaptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106546119</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Plays a role in endocytic trafficking. Required for receptor recycling from endosomes, both to the trans-Golgi network and the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Recycling endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Golgi apparatus</location>
            <location evidence="2 9">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the sesquipedalian family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918375.1">
        <property type="nucleotide sequence ID" value="XM_014062900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9B3"/>
    <dbReference type="GeneID" id="106546119"/>
    <dbReference type="KEGG" id="tsr:106546119"/>
    <dbReference type="OrthoDB" id="10261837at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13288">
        <property type="entry name" value="PH_Ses"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000250">
        <property type="entry name" value="Sesquipedalian-1 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF17">
        <property type="entry name" value="SESQUIPEDALIAN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4 9" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="17"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013918375.1"/>
        </source>
    </evidence>
    <sequence length="275" mass="30471" checksum="96D9D0905B72B62F" modified="2020-10-07" version="1">MKLNERSLSFYATCASPADHAGFLHKKGERNTAYHRRWFVLRGNMLFYFEERESREPVGVIILEGCRVELCQDSEEFAFAIRFDGPKSRTYVLAAENQAALERWVKALSRASFDYLRLVVRELEKQLEEMQAAIPGAQAAPRRRLLYRKNRTAPNLELAALPREKPSLPVCIPGKENGCALWNNSEASASLSPACREVNPCSGAKSYDGGLKPPPLPPRRRSLPSSSGGAGGPSSDGAVSLSAVCFSQLHGWYGQEIVELRKAWLEGQKEGDSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9K5</accession>
    <name>A0A6I9Y9K5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Collagen alpha-6(VI) chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106546483</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the venom Kunitz-type family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918835.1">
        <property type="nucleotide sequence ID" value="XM_014063360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9K5"/>
    <dbReference type="GeneID" id="106546483"/>
    <dbReference type="KEGG" id="tsr:106546483"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22630">
        <property type="entry name" value="Kunitz_collagen_alpha6_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000016">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000021">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF84">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="VWFA" evidence="8">
        <location>
            <begin position="298"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="8">
        <location>
            <begin position="504"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="9">
        <location>
            <begin position="810"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="102"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013918835.1"/>
        </source>
    </evidence>
    <sequence length="864" mass="94766" checksum="8161430259DDC2DE" modified="2020-10-07" version="1">MANTSTATKTVKKTLPMIAYSAGQKLTTDMPSSEKPKPAPTLQSLQASLNMIQEFMIKKQSITKNHKEMKGGLGEVKDDIKKLDDRMGKIQKTIAKNEQKIKLGFPGSDNNDLGPPGKRGNLGQQGVSGLQGIPGPLGPQGPPGHQVNAGIKGESGAKGKVGHLGPKGEQGDPGEKGQKGLPGTRGFPGFDGSNGYGLIGRKGTKGLTGSPGNPGQQGEVGDPGNPGNKGAKGIKEQRARNGTPGTKGNPGSQGPPGKMGPKGPKGITAITTCQLVNLTRGNCPCCVGKSKCPVYPTEVVFALDMSEDVTPISFETMKGIMKSLLKDLSISRSNCPTGARISVLSYNTNIKYLIRFSDFQRKDLLMEAVEGIPLERSSGQRNIGRAMRFVARNAFKHHRQGALIRKVAIFLTAGPSQDAASINTAVLEFSALDIIPTVIALREASNVREAFLTDDTGRFKLFVWESKDEQRLNYVSLCSLCYDACNPVSQCEIVDPPPININMDVAYIMDGSQDVSSEVFETMKEFVSKMLDYFVIASLPVESDKGARVALVQHAPPNFSGTELSSSVSLEFNLTTYNTKDLMKMHIQESLHQLEGSSAVGHALEWTINNVFLEANRPRKHKIIFTILGSKTSAWDREKLLKMSLEAKCRGFTMFTLALGSETDDSEMTELSSVPVEQHLLQMGKVHKFELLYALRFSQAFLKLLERELNIYPPKNMEEKCDNLDRGDNYQQAVGITDRILFPGLKHNDLLQPSELSRKNVQNKNLKIIQGPKEEQYGQIENKYFTSISMQNGTETEEHFEEVQSAKNSCSKDMDSGECENYTLKWYFHKQQNMCFQFWYGGCGGNKNRFETQEECDALCVVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA74</accession>
    <name>A0A6I9YA74_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">LOW QUALITY PROTEIN: uncharacterized protein LOC106547326</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106547326</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919930.1">
        <property type="nucleotide sequence ID" value="XM_014064455.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547326"/>
    <dbReference type="KEGG" id="tsr:106547326"/>
    <dbReference type="OrthoDB" id="10044919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008502">
        <property type="term" value="F:melatonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000056">
        <property type="entry name" value="Melatonin receptor type 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002278">
        <property type="entry name" value="Mel_1A/1B_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000025">
        <property type="entry name" value="Melatonin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF53">
        <property type="entry name" value="MELATONIN RECEPTOR TYPE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01149">
        <property type="entry name" value="MELATONIN1AR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00857">
        <property type="entry name" value="MELATONINR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="611"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="680"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="722"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="763"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="814"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="853"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="619"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="181"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="283"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013919930.1"/>
        </source>
    </evidence>
    <sequence length="928" mass="104192" checksum="F3F923843A06C825" modified="2020-10-07" version="1">MNSLPPFAPFGSAGESWEGFMERFETYLIATKNQAQTDEEKCSFFLSCCEPSMFASARAMAAPRPAYKLGWDNLMAKLKGHYAPTPSKIARSFAFRRRVQKPGESISQFLEALRTVASQCDFSNLEENLVEQFVCGVRDVHLRSRMFRHHDITLLQAVEIARAAELSEQSTADLERLQLSSQDPGHVPFTPPASQVHDIDYDDLTPSEDLEPDNVGQVRTQPRRRQPAPSAAAPHNPKASCLSCGGRHDRQTCQFRNAICRRCQRQGHIAQVCRASLPAPAFFQANERPKPQPRRQPPKPSRRGDDCHNVSEPQEVESSINQASSGSRKITANVHLAGQPCQMEVDSGSSRSLLSWDMFSRLCPAVPQTKLAPSRVLLRDYQGKLIPVLGSFPIPVKYGNFSGKLPIIIVKNALPALLGLDWFPALGLSIGGVHRINSPSIEDILQEFSDVFDGQLGCYKGTPISLSLDPQIAPIRLKARRVPFALRAKVDAELDKLLAQGVIEPVDHAPWETPIVLPVKPDGSVRICADYKSTINLALQANPYPVPVVQHLLHSLGQGCIFAKLDMAQGYQQLPVLRIVRRTSHIVGHVHAEILKRVHKRLSSTINLRHFSIIVMLLSNLIVFSXTXSLFILKKLRHSGNVFVVNLAIADLIVAIYPYPMILTSVFHNGWNLGYIHCQLSGFLMGLSVIGSIFNITGIAINRYCYICHSLKYNKLYSEKNSVCCVILIWTLTFVAIVPNFFVGSLQYDARIYSCTFSQSVSSTYTIAVVFFHFLLPITIVTFCYLRIWILVIQVRRRVKPDYKPKLKSYDFRNFITMFVVFVLFAVCWAPLNLIGLAVAVSPEMIISKIPEWLFVSSYYMAYFNSCLNAIIYGLLNQNFRREYKRILRTICTRKVFFQDSSNDAPERIKSKPSPLLTQNNLVRVGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCX8</accession>
    <name>A0A6I9YCX8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arginine vasopressin-induced protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AVPI1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in MAP kinase activation, epithelial sodium channel (ENaC) down-regulation and cell cycling.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922021.1">
        <property type="nucleotide sequence ID" value="XM_014066546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCX8"/>
    <dbReference type="GeneID" id="106549028"/>
    <dbReference type="KEGG" id="tsr:106549028"/>
    <dbReference type="CTD" id="60370"/>
    <dbReference type="OrthoDB" id="9906905at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039579">
        <property type="entry name" value="AVPI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020282">
        <property type="entry name" value="Avpi1/C8orf4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14350">
        <property type="entry name" value="ARGININE VASOPRESSIN-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15063">
        <property type="entry name" value="TC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginine vasopressin-induced protein 1/transcriptional and immune response regulator" evidence="5">
        <location>
            <begin position="36"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013922021.1"/>
        </source>
    </evidence>
    <sequence length="202" mass="22943" checksum="011781DC3394AB75" modified="2020-10-07" version="1">MEKGGEKEPPLATIWRDPSTMGTPASITRDLPQNQIAKSCTRKRASANIFQGVGLLQLRRLFRSSGDMQAEERAQLIWERAESQCITRALQQLHRRQRKLKLQANGKPLSGKGSSTRLLELQHFNHLRIEDCDTFTALDGDSKKRQKEIADGSGHYIASQSRKKEKEMDVAMYTMKNDCDLMPMVVAECNQTSLEKPQRENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE86</accession>
    <name>A0A6I9YE86_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Ly6/PLAUR domain-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">LYPD6</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922319.1">
        <property type="nucleotide sequence ID" value="XM_014066844.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922320.1">
        <property type="nucleotide sequence ID" value="XM_014066845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE86"/>
    <dbReference type="GeneID" id="106549245"/>
    <dbReference type="KEGG" id="tsr:106549245"/>
    <dbReference type="CTD" id="130574"/>
    <dbReference type="OrthoDB" id="6149028at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030550">
        <property type="term" value="F:acetylcholine receptor inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23625">
        <property type="entry name" value="TFP_LU_ECD_LYPD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000004">
        <property type="entry name" value="Ly6/PLAUR domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039457">
        <property type="entry name" value="LYPD6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31171">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31171:SF0">
        <property type="entry name" value="LY6_PLAUR DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16975">
        <property type="entry name" value="UPAR_LY6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636638" evidence="11">
        <location>
            <begin position="26"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013922319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013922320.1"/>
        </source>
    </evidence>
    <sequence length="169" mass="18868" checksum="B9B9C0B752DE772E" modified="2020-10-07" version="1">MESWPTLGWLLFLSLIADCLKGTRARDFTVKDIVYLHPSTTPYPGGFKCFTCEQAVDNYECNQWAPDVYCPRATRYCYSQHTLESNGKSVSVTKRCVPLEDCLTTGCIALQHKGLKVCTSCCEGNICNLTLPRNSSDAVFSTTTPINHTQRHWQDASIIMLGLLSLFAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEL4</accession>
    <name>A0A6I9YEL4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase theta-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922440.1">
        <property type="nucleotide sequence ID" value="XM_014066965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YEL4"/>
    <dbReference type="GeneID" id="106549343"/>
    <dbReference type="KEGG" id="tsr:106549343"/>
    <dbReference type="OrthoDB" id="2320933at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097681">
        <property type="term" value="P:double-strand break repair via alternative nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF62">
        <property type="entry name" value="DNA POLYMERASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922440.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="13298" checksum="C605858AB481E3E7" modified="2020-10-07" version="1">MGVQETDALCFIESFKSRYPGIQKFLKETVKKCTQDGFVQTIMGRHRYLPAIKETNPYKKAQAERQAVNTIIQGSAADIVKAATVNIQTQLEALPSIVKSFGHLESSFPKILSVIPKTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YES3</accession>
    <name>A0A6I9YES3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">diacylglycerol kinase (ATP)</fullName>
            <ecNumber evidence="3">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">DGKB</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1,2-didecanoyl-sn-glycerol + ATP = 1,2-didecanoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43428"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18155"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:78227"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:43429"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40323"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013922667.1">
        <property type="nucleotide sequence ID" value="XM_014067192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YES3"/>
    <dbReference type="GeneID" id="106549507"/>
    <dbReference type="KEGG" id="tsr:106549507"/>
    <dbReference type="CTD" id="1607"/>
    <dbReference type="OrthoDB" id="242257at2759"/>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20845">
        <property type="entry name" value="C1_DGKbeta_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20891">
        <property type="entry name" value="C1_DGKbeta_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000017">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.110:FF:000012">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000013">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000016">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000003">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.110">
        <property type="entry name" value="Diacylglycerol kinase alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047471">
        <property type="entry name" value="C1_DGKbeta-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047470">
        <property type="entry name" value="C1_DGKbeta_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029477">
        <property type="entry name" value="DAG_kinase_typeI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038199">
        <property type="entry name" value="DGK_typeI_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF32">
        <property type="entry name" value="DIACYLGLYCEROL KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14513">
        <property type="entry name" value="DAG_kinase_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="192"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="18">
        <location>
            <begin position="242"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="18">
        <location>
            <begin position="306"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="19">
        <location>
            <begin position="431"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="22">
        <location>
            <position position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013922667.1"/>
        </source>
    </evidence>
    <sequence length="614" mass="69636" checksum="6FA40993B70A8081" modified="2020-10-07" version="1">MMNQEKWANLSPSEFSQLQKYAEYSTKKIKDVLEEFHGNGVLAKYNPEGKQDILNQTIDLEGFKLFMKTFLEADLPDEFCEHLFVSFGNKVPHISPLVKTKSPFLPGGLRIAKSITTHPQHLSTIHIPEVIQLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGYLDNSELENIITQMMHVAEYLEWDVTELSPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGLGKQGLSCSFCKYTVHERCVSRAPASCIKTYMKSKKNTEVMHHFWVEGNCPKKCDKCHKTIKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGILKDHILPPTSICPVVLTLPPLGVSLPEERQATMKKERSSCQQTNKAADQSKVQRANSLTIDGQGLQITPVSGTQPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLNFFRDVSDFRVLACGGDGTVGWILDCIEKANLIKHPPVAILPLGTGNDLARCLRWGGGYEGENLLKILKDIENSTEILLDRWKFEVIPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFG2</accession>
    <name>A0A6I9YFG2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rhomboid-related protein 2</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">RHBDL2</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors. Known substrate: EFNB3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013922977.1">
        <property type="nucleotide sequence ID" value="XM_014067502.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013922978.1">
        <property type="nucleotide sequence ID" value="XM_014067503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFG2"/>
    <dbReference type="GeneID" id="106549767"/>
    <dbReference type="KEGG" id="tsr:106549767"/>
    <dbReference type="CTD" id="54933"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000007">
        <property type="entry name" value="Rhomboid like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF6">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="17">
        <location>
            <begin position="109"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037470-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013922977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013922978.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="33089" checksum="9D0C56B6F54B9419" modified="2020-10-07" version="1">MELERLNMEEEPETWREEERNEEEKSTCPWVKCSSLHNTVSKWMLPGEARATYLERASCVPPPIFILAISLAELSVFIYYAIWKPQIQWITLEVGIWESPLIYRPEKRKEAWRFLSYMFLHAGVEHIIGNLVLQLCLGIPLELVHKGHRVGTVYLAGVIGGSLASSICDPLLGLVGASGGVYALIGGYFMNILVNFREMIPLFGIARLLFIGLIVGTDVGFALYRRFLSPSTGIQVSFVAHIAGGLAGMSVGYVIFSNFDKSFIKDPRFWICISAFLIFVVLAVLFNVFFSPANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFQ4</accession>
    <name>A0A6I9YFQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 234 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM234</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM234 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923088.1">
        <property type="nucleotide sequence ID" value="XM_014067613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFQ4"/>
    <dbReference type="GeneID" id="106549858"/>
    <dbReference type="KEGG" id="tsr:106549858"/>
    <dbReference type="CTD" id="56063"/>
    <dbReference type="OrthoDB" id="43458at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018908">
        <property type="entry name" value="TMEM234"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28668:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10639">
        <property type="entry name" value="TMEM234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013923088.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="14562" checksum="59191BCE27A75740" modified="2020-10-07" version="1">MRCHLAGDVAILILVAILWGTTNPFLKRGTEGLEQVKRESRPQQMLAEIKFLCLNYKYMLPFIVNQCGSFIYYLALGSTDLTLAVPLCNSLALVFTVATGKVLGENIGGPRAVLGMVLVVLGVAICIAASGHRNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGZ0</accession>
    <name>A0A6I9YGZ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">M-phase inducer phosphatase 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923461.1">
        <property type="nucleotide sequence ID" value="XM_014067986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGZ0"/>
    <dbReference type="GeneID" id="106550162"/>
    <dbReference type="KEGG" id="tsr:106550162"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902751">
        <property type="term" value="P:positive regulation of cell cycle G2/M phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06617">
        <property type="entry name" value="M-inducer_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923461.1"/>
        </source>
    </evidence>
    <sequence length="115" mass="12596" checksum="DA0771CD85524758" modified="2020-10-07" version="1">MSPVTHLALDMDKLAGLGSDWDTPKRKKKLNLDFRKEWSSQDSVSSESTDSGVGPDSPMEPDVEMFPEDMESLPLRRIKSLPVRLLGSSPVLRNISHCKQCNGPGQKTSAAPDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHC9</accession>
    <name>A0A6I9YHC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Inter-alpha-trypsin inhibitor heavy chain H2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ITIH2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923280.1">
        <property type="nucleotide sequence ID" value="XM_014067805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHC9"/>
    <dbReference type="GeneID" id="106550025"/>
    <dbReference type="KEGG" id="tsr:106550025"/>
    <dbReference type="CTD" id="3698"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030212">
        <property type="term" value="P:hyaluronan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010600">
        <property type="entry name" value="ITI_HC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF14">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06668">
        <property type="entry name" value="ITI_HC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inter-alpha-trypsin inhibitor heavy chain C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923280.1"/>
        </source>
    </evidence>
    <sequence length="122" mass="14097" checksum="90D33FC5772A17B3" modified="2020-10-07" version="1">MVLLHRVWKKHPVNVDFLGIYIPPANNFSSSVHGLIGQFLQEPDVLIYNERPGQDPGKSDATMEVKGHKLTVTRGLQKDYRSDRVFGTNVQCWFVHNNGKGFLDGHYRDYLVPHLYSYLKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHX4</accession>
    <name>A0A6I9YHX4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">N-alpha-acetyltransferase 80</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">N-acetyltransferase 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">NAT6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">N-alpha-acetyltransferase that specifically mediates the acetylation of the acidic amino terminus of processed forms of beta- and gamma-actin (ACTB and ACTG, respectively). N-terminal acetylation of processed beta- and gamma-actin regulates actin filament depolymerization and elongation. In vivo, preferentially displays N-terminal acetyltransferase activity towards acid N-terminal sequences starting with Asp-Asp-Asp and Glu-Glu-Glu. In vitro, shows high activity towards Met-Asp-Glu-Leu and Met-Asp-Asp-Asp. May act as a tumor suppressor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-terminal L-aspartyl-L-aspartyl-L-aspartyl-[protein] + acetyl-CoA = N-terminal N-acetyl-L-aspartyl-L-aspartyl-L-aspartyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57328"/>
            <dbReference type="Rhea" id="RHEA-COMP:14863"/>
            <dbReference type="Rhea" id="RHEA-COMP:14864"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:141602"/>
            <dbReference type="ChEBI" id="CHEBI:141604"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal L-glutamyl-L-glutamyl-L-glutamyl-[protein] + acetyl-CoA = N-terminal N-acetyl-L-glutamyl-L-glutamyl-L-glutamyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57324"/>
            <dbReference type="Rhea" id="RHEA-COMP:14865"/>
            <dbReference type="Rhea" id="RHEA-COMP:14866"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:141603"/>
            <dbReference type="ChEBI" id="CHEBI:141606"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923963.1">
        <property type="nucleotide sequence ID" value="XM_014068488.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923964.1">
        <property type="nucleotide sequence ID" value="XM_014068489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHX4"/>
    <dbReference type="GeneID" id="106550553"/>
    <dbReference type="KEGG" id="tsr:106550553"/>
    <dbReference type="CTD" id="24142"/>
    <dbReference type="OrthoDB" id="329272at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905502">
        <property type="term" value="F:acetyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000061">
        <property type="entry name" value="N(alpha)-acetyltransferase 80, NatH catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039840">
        <property type="entry name" value="NAA80"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13538">
        <property type="entry name" value="N-ACETYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13538:SF4">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="11"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="236"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="266"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013923963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013923964.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="36494" checksum="64138CA0D630982C" modified="2020-10-07" version="1">MGSVSEMLTAVPLHKRPDLLEACADLINEEWKRSRTSRIHSLQKSSDNFPMCLVLIRTLRVTEETAEGKTSNTQCQLLGHARLSRVIGQSNSLFVETVVVAKALRGKGYGRKLMEAVENFAKAHRYQHLYLTTHDKQHFYAHLGYSLSKPVQWAGFISSLVPMEFLENFSTPQHKTELPAQSGKHNTNASYQSRSGANLPPSPHAAPLSSSPFCSVAPPSLPSPCSYHSGTATISPHLPLPGPHPSSAQSRNALVHSSPFASAAPAPLPSPPPPPPPLPLPVPPPRAFLVVQPNSGKPSSSRVLENTFEEQTLFETPYRDLKGKPIFWMKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIC1</accession>
    <name>A0A6I9YIC1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60S ribosomal protein L8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106550548</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923957.1">
        <property type="nucleotide sequence ID" value="XM_014068482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIC1"/>
    <dbReference type="GeneID" id="106550548"/>
    <dbReference type="KEGG" id="tsr:106550548"/>
    <dbReference type="OrthoDB" id="10267824at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000006">
        <property type="entry name" value="60S ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013923957.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="16322" checksum="8B0517494CC28CBA" modified="2020-10-07" version="1">MPEGTIICCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGIVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIR4</accession>
    <name>A0A6I9YIR4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Amine oxidase</fullName>
            <ecNumber evidence="15">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106550339</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a secondary aliphatic amine + O2 + H2O = a primary amine + an aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:26414"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:58855"/>
            <dbReference type="ChEBI" id="CHEBI:65296"/>
            <dbReference type="EC" id="1.4.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013923710.1">
        <property type="nucleotide sequence ID" value="XM_014068235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIR4"/>
    <dbReference type="GeneID" id="106550339"/>
    <dbReference type="KEGG" id="tsr:106550339"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097621">
        <property type="term" value="F:monoamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000005">
        <property type="entry name" value="Amine oxidase [flavin-containing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050703">
        <property type="entry name" value="Flavin_MAO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563:SF16">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0274">FAD</keyword>
    <keyword evidence="4 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="16">
        <location>
            <begin position="16"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="36"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013923710.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="58787" checksum="9AC9E929E07E42A4" modified="2020-10-07" version="1">MSSDHKCDVIVIGGGISGLSAAKLLYDSGLDVVVLEAQDRVGGRTYTVRNKHVKYVDLGGAYVGPTQNRVLRLSKELGLETYKVNEVEHLIHHVNGKSYPFKGPFPPIWNPITYLDYNNLWRTLDEMGKEIPNEAPWKAPRAEEWDKITMEQLLDKVCWTNAAKRFGTLFVNVDVTSEPHEVSALWFLWYVKQCGGTARIFSTTNGGQERKFIGGSGQISEKIMEYLGKRVKLEKPVTYVDQTGGNVIVETLDHERYEAQYVISAIPPVLSLKIHFNPPLPSMRNQMINRIPMGSVIKCIVYYKDAFWKKKDYCGSMIIEDEGAPIGLALDDTKPDGSFPAIIGFILARKCRRLINLTKEERKTQICELYAKVLGTQEALRPVHYEEKNWCKEQYSGGCYTAYFPPGIMTQYGRILREPVGKIFFAGTETASEWSGYMEGAVQAGERAAREVLQSMGRYSGEIWKSEPESHDVPALPIIATFWERNLPSVPGLLKLMGFSVFFTSVAAAGFFAYKKDLLVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ49</accession>
    <name>A0A6I9YJ49_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein PXR1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924236.1">
        <property type="nucleotide sequence ID" value="XM_014068761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ49"/>
    <dbReference type="GeneID" id="106550790"/>
    <dbReference type="KEGG" id="tsr:106550790"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924236.1"/>
        </source>
    </evidence>
    <sequence length="48" mass="6406" checksum="92D0366884A96B48" modified="2020-10-07" version="1">KERKKERRKKEKRKEERKKEREKEIRKKEKRKEERKKERKEGRKKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ90</accession>
    <name>A0A6I9YJ90_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="5">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Meiotic recombination protein SPO11-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SPO11</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013924312.1">
        <property type="nucleotide sequence ID" value="XM_014068837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJ90"/>
    <dbReference type="GeneID" id="106550854"/>
    <dbReference type="KEGG" id="tsr:106550854"/>
    <dbReference type="CTD" id="23626"/>
    <dbReference type="OrthoDB" id="5377392at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000387">
        <property type="entry name" value="DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013048">
        <property type="entry name" value="Meiotic_Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01551">
        <property type="entry name" value="SPO11HOMOLOG"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="15">
        <location>
            <begin position="76"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="16">
        <location>
            <begin position="184"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="14">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013924312.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="40741" checksum="A384E326A6FDB9A3" modified="2020-10-07" version="1">MKLYLVTSFSSEVLRAIENVIEDVIMSLSRNKAPILILRNRSDWGNIHFTEAVGLQMISNCTTQQIRSDSPKSAPKFALCLKILSIIYKMVQTNTYATKRDIFYTDTVLFGNQRVVDNIINDICCMLKIPRRTLHILSTSKGCIAGNLSYIEEDGTKVNCTCSTTTPVPSNVEGIRNLITDAKFILIIEKDATFQRLLDDNFCCKMSPSIIITGKGVPDINTRLLIRKLWDTCHIPIFALMDADPHGIEIMSIYKYGSVSMSFEAHRLTVPAVRWLGLLPSDIYRLNVPKSALIPLTKEDQSKLTALQARPYTAHESAWKKELDIMETSKMKAEIQALASFSTDYLSRIYLPNKLQFGGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKX3</accession>
    <name>A0A6I9YKX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Long-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ACADL</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>decanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-decenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:48177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dodecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47296"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:47297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>octanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-octenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48180"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:48181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H(+) = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:43449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:47317"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924425.1">
        <property type="nucleotide sequence ID" value="XM_014068950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKX3"/>
    <dbReference type="GeneID" id="106550960"/>
    <dbReference type="KEGG" id="tsr:106550960"/>
    <dbReference type="CTD" id="33"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004466">
        <property type="term" value="F:long-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019254">
        <property type="term" value="P:carnitine metabolic process, CoA-linked"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042758">
        <property type="term" value="P:long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000020">
        <property type="entry name" value="Long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF20">
        <property type="entry name" value="LONG-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="18">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="45"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="16">
        <location>
            <begin position="154"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013924425.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="34104" checksum="59C3A272A4C6DA78" modified="2020-10-07" version="1">MYANCTGPGFSLHSDIVMPYIANYGSKEQIERFIPEMAAGRCIGAIAMTEPGAGSDLQGVKTFAKKEGNDWILNGSKVFITNGWMCDLVIVVAVTNREARSPAHGISLFLVENGMEGFVKGRKLEKIGLKAQDTAELFFEDVRLPASALLGQENKGFYYLMSELPQERLLIGDIAIASCEFMFEETRNYVKQRKAFGKTISHLQTVQHKLAELKTQICVSRTFLDNCIDLHSRKCLDSATASMAKYWASDLQNNVATQCVQLHGGWGYMWEYPIAKSFVDSRVQPIYGGTNEIMKELIARDIVSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLJ8</accession>
    <name>A0A6I9YLJ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Electron transfer flavoprotein subunit beta</fullName>
            <shortName evidence="8">Beta-ETF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">ETFB</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase. Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purely structural role.</text>
    </comment>
    <comment type="function">
        <text evidence="8">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interacts with ACADM.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924650.1">
        <property type="nucleotide sequence ID" value="XM_014069175.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013924651.1">
        <property type="nucleotide sequence ID" value="XM_014069176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLJ8"/>
    <dbReference type="GeneID" id="106551149"/>
    <dbReference type="KEGG" id="tsr:106551149"/>
    <dbReference type="CTD" id="2109"/>
    <dbReference type="OrthoDB" id="276685at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045251">
        <property type="term" value="C:electron transfer flavoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="9">
        <location>
            <begin position="28"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013924650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013924651.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="28146" checksum="1DB7C1D1AFB8C5E9" modified="2020-10-07" version="1">MAAAGLRAMVGVKRVIDYAVKVRVKPDKSGVVTDGVKHSMNPFCEIAIEEAVRLKEKKLVSEIIAVSCGPQQCQETIRTALAMGADRGVHVEIPAKDYESLTPFQVSKMLAALAKKENVNLLLLGKQAIDDDCNQTGQMTAAFLDWPQGTFASEVTLDGDWLKVEREVDGGLETVRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKMEMVKPSDLGVDLTPRVKTLSVEDPPQRKAGVKVETVEDLVAKLKEIGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLM9</accession>
    <name>A0A6I9YLM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Copine-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Copine III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CPNE3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Calcium-dependent phospholipid-binding protein that plays a role in ERBB2-mediated tumor cell migration in response to growth factor heregulin stimulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Monomer. Interacts with ERBB2 (preferentially with the tyrosine phosphorylated form); this interaction occurs at the cell membrane and is increased in a growth factor heregulin-dependent manner. Interacts with SHC1; this interaction may mediate the binding of CPNE3 with ERBB2. Interacts with RACK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copine family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924685.1">
        <property type="nucleotide sequence ID" value="XM_014069210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLM9"/>
    <dbReference type="GeneID" id="106551172"/>
    <dbReference type="KEGG" id="tsr:106551172"/>
    <dbReference type="CTD" id="8895"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038128">
        <property type="term" value="P:ERBB2 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000042">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000099">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF22">
        <property type="entry name" value="COPINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="19">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="19">
        <location>
            <begin position="112"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013924685.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="59249" checksum="1B05D379E60891BB" modified="2020-10-07" version="1">VSCNNLLDKDISSKSDPLCVLLQYTSGQQWYEVDRTERIKNSLNPKFSKKFQIDYYFELVQKLKFGVFDIDNTTVQLNDDDFLGEFECTLAQIVSSKTLTRPLLLRNGKPAGKGVITISAEEIKDNRVVLLEAEARKLDNKDLFGKSDPYLEFHKQTSEGKWVMVHRTEVIKNNLNPVWRPFKISLNSLCYGDMDKAIKVECYDYDNDGSHDLIGTFQATMSKLKEASRHSPIEFECINEKKRQKKKSYKNSGIVSFKHCEVITECTFLDYVMGGCQLNFTVGIDFTGSNGDPRSSDSLHYISPNGVNEYLSAIWSVGMVIQDYDADKMFPAFGFGAQIPPSWQVSHEFPLSFNPSNPFCNGVQGIVDAYRVCLPQIRLYGPTNFSPIINHVARFAAAASQQRTASQYFILLIITDGVITDLDETRSAIVNASKLPMSIIIVGVGGADFSAMEFLDSDSGALRSRSGEAAVRDIVQFVPFRHFHNAPKEALAQSVLAEVPQQVVGYFSMYKLQPPNKPSAKQEQQKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLN4</accession>
    <name>A0A6I9YLN4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division cycle-associated protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CDCA3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924690.1">
        <property type="nucleotide sequence ID" value="XM_014069215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLN4"/>
    <dbReference type="GeneID" id="106551148"/>
    <dbReference type="KEGG" id="tsr:106551148"/>
    <dbReference type="CTD" id="83461"/>
    <dbReference type="OrthoDB" id="6337960at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038832">
        <property type="entry name" value="CDCA3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34756">
        <property type="entry name" value="CELL DIVISION CYCLE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34756:SF1">
        <property type="entry name" value="CELL DIVISION CYCLE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924690.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="30808" checksum="3CCAAFC513B7CCDA" modified="2020-10-07" version="1">MAAPSLGGFLRSQTVAMGASESFPATPTCTPLLNKHLKHVNDPRSPTTGIPRTPIEVQSSPKDNTQFPKQGELVAKSQHESWDPRSPTPGISRTPLKDVLADTINYPVKLFCENFMGQNREEELSQQESQHQDQKSEFTFGMEERTAKSIVSSEEIFLGDSQQAEGGNDQFSLVSVVAATKPVQSARIIHPTGSKSVKRRISNKTMAMPTGTGRSPLSILQGDNSPISLAFHQSKRTSSVTDNQREPKDGNLNSGPNFPADSCGWDRMNKENRQCRWVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLW0</accession>
    <name>A0A6I9YLW0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Poly [ADP-ribose] polymerase 15-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106551607</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925217.1">
        <property type="nucleotide sequence ID" value="XM_014069742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLW0"/>
    <dbReference type="GeneID" id="106551607"/>
    <dbReference type="KEGG" id="tsr:106551607"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052056">
        <property type="entry name" value="Mono-ARTD/PARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453">
        <property type="entry name" value="PARP/ZINC FINGER CCCH TYPE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453:SF89">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Macro" evidence="6">
        <location>
            <begin position="14"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013925217.1"/>
        </source>
    </evidence>
    <sequence length="81" mass="8374" checksum="335D9A10149C1A9E" modified="2020-10-07" version="1">MAVTGSFGSISTSALGSNEMQFGSITFQSVTGDIVMEKTDVIVNVTNENFSSKAGVSKAILEAAGPEIEAEYARLGTILAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMG0</accession>
    <name>A0A6I9YMG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial enolase superfamily member 1</fullName>
            <ecNumber evidence="3">4.2.1.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-fuconate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ENOSF1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:22772"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:21291"/>
            <dbReference type="ChEBI" id="CHEBI:37448"/>
            <dbReference type="EC" id="4.2.1.68"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mandelate racemase/muconate lactonizing enzyme family. ENOSF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.68" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013925578.1">
        <property type="nucleotide sequence ID" value="XM_014070103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMG0"/>
    <dbReference type="GeneID" id="106551939"/>
    <dbReference type="KEGG" id="tsr:106551939"/>
    <dbReference type="CTD" id="55556"/>
    <dbReference type="OrthoDB" id="14161at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050023">
        <property type="term" value="F:L-fuconate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03324">
        <property type="entry name" value="rTSbeta_L-fuconate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000007">
        <property type="entry name" value="Mitochondrial enolase superfamily member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034610">
        <property type="entry name" value="L-fuconate_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00111">
        <property type="entry name" value="L-fuconate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="10">
        <location>
            <begin position="201"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013925578.1"/>
        </source>
    </evidence>
    <sequence length="440" mass="49319" checksum="F3BDA711BD1B5DEB" modified="2020-10-07" version="1">MGRGRIRALAVSDVRFPTSLAQHGSDAMHPDPDYSAAYVVIETDAPNGLKGCGFTFTLGKGTEVVISAVHALSIHIINKDLDDIISDFRGFYRQLTSDGQLRWIGPEKGAVHLATAAILNAVWDLWAKQEGKPLWKLLVDMDPKQLLSCIDFRYITDALTEEEAFSILQSGLAGKKEREEQMLKHGYPAYTTSCAWLGYPDHCLKQLCTEALKDGWTRFKVKVGKDLQDDIRRCKLVREMIGPENILLLDANQRWEVEEAIDWVTELAEFKPLWIEEPTSPDDVLGHATIAKALAPLGIGVATGEQCHNRVMFKQFLQAQSLSYLQVDSCRLGSVNENLSVLLMAKKFQIPVCPHAGGVGLCELVQHLIIFDYISVSGSLEKRMCEYVDHLHEHFKSPVVIMNASYMPPQDAGYSSEMKEDSVKKYLFPQGEIWQKFLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMI1</accession>
    <name>A0A6I9YMI1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 2, facilitated glucose transporter member 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106551719</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925341.1">
        <property type="nucleotide sequence ID" value="XM_014069866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMI1"/>
    <dbReference type="GeneID" id="106551719"/>
    <dbReference type="KEGG" id="tsr:106551719"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013925341.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="19031" checksum="FAD5242A2A522A8B" modified="2020-10-07" version="1">MGSGTLIYSSPLYILEISSLNMRGALVTTLFLFFMWGYLLVQILTSPWIWGNEENFSLLVGVTAIFAIISSIFLVPSPESPRFLFLQRNDQEKARQVLKKLRGKEDVEDEMLELYQEHLAESNQKNMTVWKLLRFGSLRWHLITVVVLVSGSRFIQTNAVRDFPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMJ2</accession>
    <name>A0A6I9YMJ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">HEPACAM family member 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HEPACAM2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925613.1">
        <property type="nucleotide sequence ID" value="XM_014070138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMJ2"/>
    <dbReference type="GeneID" id="106551969"/>
    <dbReference type="KEGG" id="tsr:106551969"/>
    <dbReference type="CTD" id="253012"/>
    <dbReference type="OrthoDB" id="9872799at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052280">
        <property type="entry name" value="HEPACAM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44888:SF1">
        <property type="entry name" value="HEPACAM FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44888">
        <property type="entry name" value="HEPACAM FAMILY MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026829500" evidence="13">
        <location>
            <begin position="32"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="149"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="241"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925613.1"/>
        </source>
    </evidence>
    <sequence length="466" mass="52133" checksum="05C8E97C6F6EC2AC" modified="2020-10-07" version="1">MALAQNKLLFNTMVCGLQIFVLCLLLGNSSALKMTIPSHRVNGIEGQPLRLEVVYNFNVAISDIQIIWLFEKPPTDPKYLLSSVNQSVVPDIQYRHKFTLLPPNASLLINSLHRSDEGNYIVKINIDGNETKSASEKIQVTVDVPLTKPVIYMEPSLGVVEKKGNITLKCMVEKGTRVVYQWMKNENPIDDSSNGSMTINKDTLSISPVFKEAIGNYSCLVTNPISAMKSDVFMPTIFYGPYGITINTAEGQKVGKVFTLDKGDAVQLYCSADSNPPNIYSWILKSNNSMYPLQYGRLLEIASEEVTQKTEYICTAYNNMTGTYDETHILVIVIPKGLDQPAQKGKHLSPLAVITGISVFLTVAICIFLLWKRFQPHKALQRKLRRRAPRIKARYRKGQSVTGHQSAGDDFGVYEFIEYTSSRGTPWPPRRRGAADSDVGQSQNTVTIYDVVQHVPEQNEEQQDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPI4</accession>
    <name>A0A6I9YPI4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Diencephalon/mesencephalon homeobox protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DMBX1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925819.1">
        <property type="nucleotide sequence ID" value="XM_014070344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPI4"/>
    <dbReference type="GeneID" id="106552145"/>
    <dbReference type="KEGG" id="tsr:106552145"/>
    <dbReference type="CTD" id="127343"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000125">
        <property type="entry name" value="diencephalon/mesencephalon homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052488">
        <property type="entry name" value="DMBX_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46639">
        <property type="entry name" value="DIENCEPHALON/MESENCEPHALON HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46639:SF2">
        <property type="entry name" value="DIENCEPHALON_MESENCEPHALON HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="165"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013925819.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="36197" checksum="C889FEE85CB136D4" modified="2020-10-07" version="1">PYFLSSNGCTFQDIILEARYGSQHRKQRRSRTAFTAQQLEALEKTFQKTHYPDVVMRERLAMCTNLPEARVQVWFKNRRAKFRKKQRSLQKEQLQKQKDSEANHSEGKADTPVLETPTPTPDADKSLSLPREVNAELSLTLSEHSASESAAEDQTDKEEEFKIFLEENKEEKSPGEENKVLNSKRASPKAESPVGTAATASSSSGMSQAHSYSSSPLSLFRLQEQFRQHMAATNNLVHYSSFEMGNPSSMPYLGMNVNMAPLSSLHCQSYYQSLSHAQQVWSSPILQASSSLPSLNSKTTSIENLRLRAKQHAASLGLDTLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPM8</accession>
    <name>A0A6I9YPM8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribonuclease 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925864.1">
        <property type="nucleotide sequence ID" value="XM_014070389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPM8"/>
    <dbReference type="GeneID" id="106552177"/>
    <dbReference type="KEGG" id="tsr:106552177"/>
    <dbReference type="OrthoDB" id="67027at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013925864.1"/>
        </source>
    </evidence>
    <sequence length="421" mass="47739" checksum="DC09C4C163CDA5D5" modified="2020-10-07" version="1">MRNIFNVQFFSPPAANVPTSSLILTQRNVTKPFSPASSSSSPSWVMSFHSGRGCPRGRGGATVRSSSQTFRPQNLRQLHPQQSSMQYQYDQQPPTTSIYANPPTPNYAPPRPDFVPYPPPIPPSPQGSITQCPPRPPFSGPQMRQTFPLPPCFPPAPPPVPNPSNPPVLANAAGQNTFPYMMPPPSIPHPLPPPVMPQQVNYQPAYSPGFSQQTFSPPNFSNYQHSANSYQSNSGSASSGGSTSGNFRHPSEYLVEKSQTDRRSPERSKYYDEHRHRDHCHGHGDRHRSTNHGDRRDRGRSPDRRRAESSRHKSDYVRGRTPPRHRSYEWYSRSPSQDRKRPRWEEDRDRWSENANREKSYTSIKDKEPEEATGEKNEEEDEDLLKPVWVRCTHSESYYSNDPMDQVVCIPRISGNQLLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ00</accession>
    <name>A0A6I9YQ00_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106552466</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18373"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:18374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926231.1">
        <property type="nucleotide sequence ID" value="XM_014070756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ00"/>
    <dbReference type="GeneID" id="106552466"/>
    <dbReference type="KEGG" id="tsr:106552466"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000007">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000001">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF28">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 3-KINASE C2 DOMAIN-CONTAINING SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="7">
        <location>
            <begin position="701"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1068"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1068"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1090"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926231.1"/>
        </source>
    </evidence>
    <sequence length="1136" mass="127348" checksum="9C109EAA6D95D4F9" modified="2020-10-07" version="1">MDICFHRMAQISSSDEFKQRSSPILDPARPKDVDKEEALQMEAEALAKMQKERVVVGSRQKSDTLSYSWPSVQSCRKQDHDLMVFPESDGKKKVDDKLNSIDIDKLTRAELEKILLEENLESNKPSTLSAPQILGASSSQFYSHPVAQSGQWTSSLPGSSTSAGSSVYPVPSFNKQVGIFQNGFHPGVSAYQSTKPAYLCLPERSQYISYPLPFTSTFHPQGSVPMYHSTLTPEMAKVFDKIASTSEFQKNGKSKTDLEMTGAKPTISLPISESSNDISKFDWLDLDPLSKPKVDNIDIFYTSEDGGKITNAKVVEDPWDAVLLEEKSPGKGHLERKLSGKSASGATVTRSQSLNIRTAQLAKLQGQTAQCDLVYTFFHPLPRDEKAEGIDGITRCTDTGPSSPTSGKVGGEVKLSISYRNKTLFIMVMHIKDLVDFPSLPFDIIYKIPQAANNSTCNSIDTLEKDSRERLLSIISKEGTYGLSRKDKAFLWDKRHYCYTHAGSLPKILASAPNWDLTNLPEIYLLLQQWPPLPPLTALELLNANFADQEVRTKAVQWIEAINDDELADILPQFVQALKYETYLDNSLVRFLLARALGNIRIAHYLYWLLKDTLHDTKHGIRYEHILGAFLSICGKSLREELERQSRLVQLLGMVAEKVKQTSGSARQMVLQSGMERVQSFFLKNKCRLPLSPSLVAKELNVKACSYFSSNAVPLKIALVNADPLGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLQEGLDLRMVMFKCLATGKDRGMVELVPASDTLRKIQVEYGVTGSFKDKPLAEWLRKYNSTEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRSTGHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQANLFLNLLSLMMSSGLPELSGIQDLKYVQDALQPQTTDAEATIFFTSGVLVCTTTDTEDPLCAQASKYVRRHEFNIRNQMEGESINEYVVALRKAAVYCEFRDLDELILDCIICGMRDITLQRRLPAKSNLSLQIALDKARASESAAKSTETLQRQSAPQDAQKSTRVHRETTKPEDSESKDDDSYCMRSSRTKLAQKFNSQVPPCASCGRDHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQE3</accession>
    <name>A0A6I9YQE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">26S proteasome non-ATPase regulatory subunit 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">26S proteasome regulatory subunit p28</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Gankyrin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PSMD10</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Part of transient complex containing PSMD10, PSMC4, PSMC5 and PAAF1 formed during the assembly of the 26S proteasome. Stays associated throughout the assembly of the PA700/19S RC and is released upon association with the 20S core. Interacts with PSMC4. Interacts with RB1. Interacts with CDK4. Interacts with MDM2. Interacts with RELA. Associates with a CDK4:CCND2 serine/threonine kinase complex. Interacts with ARHGDIA and increases the interaction between ARHGDIA and RHOA, hence promotes ARHGDIA inactivation of RHOA and ROCK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925875.1">
        <property type="nucleotide sequence ID" value="XM_014070400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQE3"/>
    <dbReference type="GeneID" id="106552187"/>
    <dbReference type="KEGG" id="tsr:106552187"/>
    <dbReference type="CTD" id="5716"/>
    <dbReference type="OrthoDB" id="1577640at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000149">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF16">
        <property type="entry name" value="ANKYRIN-1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="143"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="176"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925875.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="24694" checksum="9E7EFA6FD74FAE97" modified="2020-10-07" version="1">MEGQGGGGSVSNVELCNLAYAGQLEEVKERLHANRSLATRADQDNRTALHWACSAGHLDIVDFLLSLGVPVNDKDDAGWTPLHIAASAGRDEIVKALLQKGAQVNATNQNGCTPLHYAASKNKQEIALMLLENKANPDAKDHLASTPLHRAASKGNQKMIQILLKHNASVNLQDSEGNTPLHLACDEARVDEAKLLVSHGASIDIENKEEKTPLKVAKGGLASILRRLAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQQ8</accession>
    <name>A0A6I9YQQ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein Red-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925985.1">
        <property type="nucleotide sequence ID" value="XM_014070510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQQ8"/>
    <dbReference type="GeneID" id="106552283"/>
    <dbReference type="KEGG" id="tsr:106552283"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013925985.1"/>
        </source>
    </evidence>
    <sequence length="62" mass="7737" checksum="0BB8EC484F7A2AC8" modified="2020-10-07" version="1">LLLPPPLPPPPPLPPPPRERGEKERGREREREREREREREKERERKRERERERIRIKFSNPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRX0</accession>
    <name>A0A6I9YRX0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable helicase senataxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106552567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926360.1">
        <property type="nucleotide sequence ID" value="XM_014070885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRX0"/>
    <dbReference type="GeneID" id="106552567"/>
    <dbReference type="KEGG" id="tsr:106552567"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926360.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="22580" checksum="9F4CADE3D9414720" modified="2020-10-07" version="1">MIQDAQRRGNIIKTSKNTYKDSAMKILKSRSFSHSATATLERGGQGAPAVLSGAKPQALPSPDTQRPPVSLPTPAVKRTPQGTLNHRPPPVAQVKPIQDRPQDPRLFRRAESALKATGCKEGQAPASGPSSVQKPSGPWTVQSHRNSTGALGETSNRVGPVPSAPLPSRGPSAETSLPTRNVHPSYPRRILEVPQEKDNSTEPKKRRTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRZ3</accession>
    <name>A0A6I9YRZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TELO2-interacting protein 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TTI1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the tti1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927062.1">
        <property type="nucleotide sequence ID" value="XM_014071587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRZ3"/>
    <dbReference type="GeneID" id="106553146"/>
    <dbReference type="KEGG" id="tsr:106553146"/>
    <dbReference type="CTD" id="9675"/>
    <dbReference type="OrthoDB" id="49511at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000229">
        <property type="entry name" value="TELO2-interacting protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000240">
        <property type="entry name" value="TELO2-interacting protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016441">
        <property type="entry name" value="Tti1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24176">
        <property type="entry name" value="TPR_TTI1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005250">
        <property type="entry name" value="UCP005250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="9">
        <location>
            <begin position="89"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="10">
        <location>
            <begin position="824"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="880"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="882"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927062.1"/>
        </source>
    </evidence>
    <sequence length="1144" mass="124879" checksum="F11A0B284E6AC69E" modified="2020-10-07" version="1">MEMPGRKEAKRSPHCLLSSFSRDRWSSGQSHCRRRACRHRKAVILTCRAFSCSARALSVGQGGWRSNEPESGAVAAAAMAIFDTPQEAFGALRPSCVQLTQAQTVENVERLHSQLRVVSDSALQELQEYVLFPLRFALKTPGPKREGVVRSVVQCVGFVLSSTCVKKADLLLELFSELCVCLASPSNPSQLAPLSEELKQDVGQALLALLHSAYGDVLLSLYQPSSLPQLGFAVSLLLALAEQEKARQVKITALRCLQALLLQCDCPEDHRSLEKEERRQCGDLFASFLPGISISLSKIIAADAKQGHAVTVSAIKLFSTAVGLVMADEQLAEIPAERKKPASGQSKMEALVVHRDAAWTRNTASKLSILIKKVAASASVHPHWKVRRELVEMARLLLTTCGRSLVDSAGQLLKALVGLVNDESPEVQRPSERALKEMSAQERVAGNRLLADILSEDLHSLATALPRLMTSQNDQGKVATLNLALGYLKLLGPKVSVVLHSASHLQRLSKALMQVLELEVKDVKVVEERSCAPEAFPQPARGSLQRKYFRFFTDDRIFPLLQQICRALGTYGNLYLLVDHFMDLYRESTAYRKQAAMVVNELIAGAAGQEAQNTSLSSEDLVGIVTSVLEEYVDSGNWHLATRLEVQESWDESAATEARPLAVTYGAAHAPLVSPGSSPTLRCMNSNIWQVCIQLEGISTFASALGKDFRLLLISALYPVLEKAGDGTLLVSQTAKGTLADICQACGYLSVPELLRQNADYLVNGVSLHLRHPADEPHALQVLEAMLRHSDAQGAPLVEDLVEDILARLDQCHSERASSFLGALRTLMASLASWFGPEQETHHKPEGGSSCPRTPRPADLPTAAEMEEFFLAYVKQKQMAEGDVEEEEEEEDPAVPPDAPEGSPEADRPLPLQAKMAKDVLERAVHLLPSKSLAVRLKALEVLELGVMVLGPHENVLLPLAHRAWPALVCRLINDDPLAVLRAFQVLCTLGAHSGDFLRSRFSKDVLPKLAASLVAQAPTSAHAGPVYSHTLAFKLQLAVLQGLGSLCQALDLGENDLNTVVDACLPYLSARQPGRLQEAARSVFLRLMEVDPDAIWFFLCNVWCPRNLEAPHPCLRPVKLAGSEKTRNEFTDNVQSLLDQLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV01</accession>
    <name>A0A6I9YV01_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">A-kinase anchor protein 17A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">AKAP17A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928132.1">
        <property type="nucleotide sequence ID" value="XM_014072657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV01"/>
    <dbReference type="GeneID" id="106554058"/>
    <dbReference type="KEGG" id="tsr:106554058"/>
    <dbReference type="CTD" id="8227"/>
    <dbReference type="OrthoDB" id="1918237at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12264">
        <property type="entry name" value="RRM_AKAP17A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056852">
        <property type="entry name" value="AK17A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12484:SF2">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 17A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12484">
        <property type="entry name" value="B-LYMPHOCYTE ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25015">
        <property type="entry name" value="RBD_AKAP-17A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="147"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="612"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="645"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="661"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="672"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928132.1"/>
        </source>
    </evidence>
    <sequence length="681" mass="79666" checksum="DF2194D0A78219D8" modified="2020-10-07" version="1">MAAATIVHDTSEAVELCASYGLYLKPITKMTISVALPQLKQPGKSISNWEVMERLKGMVHTHQFSTLRISKSTMDFIRFEGEVENKSLVKSFLSCLDGKTIKLSGFSDILKVRAAEYKIDFPTRHDWDSFFRDAKDMNESLPGERPDTIHLEGLPCKWFALKDSGSEKPSEEALIKVFSKFGEIRNVDIPMLDPYREEMTGRNFHTFSFGGHLNFEAYIQYEEYAGFIKAMNALRGMKLMYKGEDGKAVACNIKVSFDSTKHLSDASIKKRQLERQKLQELEKQREEQKRKEKEAEERQKEEERKQKELEELEREKKREEKLRKREQKQKDREIRRNKKRLEKLQAEEQKKLQEKIKLEERKLLLAQRNLQSIRLIAELLSRAKAIKLLEQEHSEEKIRLQQLEEKRKLQEAELRRVEEEKERALGLQRKERELREKLLKNLMSKKMEIIPVKKYDPNTVESPTWKSLASVPHCITSTSVQPILSQPLHVCQSSIAANESCPHPKYLNGSFHEESLQETMVNICNKDKVSDESSLDVISSVPANQQHNSTLDHEKILYKNSLSEQGKCNREPSKGRSHSYRDRDVCNHQGKNKIEKSRHRRDLSSEDEKCRKERRPYKKHSRKSSSPHQRSPDLEYARQKQTFSSDRDQDTRGRSDSNKSTGKKQKHRKRSLSNQRSTWSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWM6</accession>
    <name>A0A6I9YWM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Collagen alpha-5(IV) chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106554844</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929053.1">
        <property type="nucleotide sequence ID" value="XM_014073578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWM6"/>
    <dbReference type="GeneID" id="106554844"/>
    <dbReference type="KEGG" id="tsr:106554844"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1082">
        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929053.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="15127" checksum="3C7B9338E3D16607" modified="2020-10-07" version="1">MGLNFQGPKGEKGEQGLQGPPGPPGQIGEQKRINDVEFQKGDQGDTGDPGPPGSPGSPGPPGPPGGGKGEKGEQGEPGKRVSNFLEPNIYLSKPFDRLCERGPPGPQGKGGEQGFPGERGQKGYNNCTYAVSLFMLKLASRSLVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYS7</accession>
    <name>A0A6I9YYS7_9SAUR</name>
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        <submittedName>
            <fullName evidence="14">LOW QUALITY PROTEIN: potassium channel subfamily T member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106554550</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928730.1">
        <property type="nucleotide sequence ID" value="XM_014073255.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554550"/>
    <dbReference type="KEGG" id="tsr:106554550"/>
    <dbReference type="OrthoDB" id="257992at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005228">
        <property type="term" value="F:intracellular sodium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF14">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY T MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="12">
        <location>
            <begin position="49"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium-activated potassium channel BK alpha subunit" evidence="11">
        <location>
            <begin position="134"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013928730.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="45515" checksum="94504CCABFA357C3" modified="2020-10-07" version="1">MPHKEEGSQTLGEDLLACCFFCLQFEELVYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKXVWFSLLSLHLTQTKWEGQESPEQWQRMYGRCSGNEVYHIRMGDSKFFMEYEGKSFTYASFHAHKKYGVCLMGVKKENKSILLNPGPRHIMTASDTCFYINITKEENSAFIFKQEEKQKQKFMGKGIYEGPSRLPVHSIIASMGTVAMDLQNTECHPNHSSKLTLPSENGSGKRRPSIAPVLEVADASSLLPCDMLSDQSEDEVTQSDDDVLTIIESGLRAANRISVKKCGGNLIRVGEVQLSKTSVYISDVVHLNIGRPPCKKTNKKDNRNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZE0</accession>
    <name>A0A6I9YZE0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12 13">G-protein coupled receptor family C group 5 member C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">GPRC5C</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929240.1">
        <property type="nucleotide sequence ID" value="XM_014073765.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013929241.1">
        <property type="nucleotide sequence ID" value="XM_014073766.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013929242.1">
        <property type="nucleotide sequence ID" value="XM_014073767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZE0"/>
    <dbReference type="GeneID" id="106555004"/>
    <dbReference type="KEGG" id="tsr:106555004"/>
    <dbReference type="CTD" id="55890"/>
    <dbReference type="OrthoDB" id="9880600at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15277">
        <property type="entry name" value="7tmC_RAIG3_GPRC5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051753">
        <property type="entry name" value="RA-inducible_GPCR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511">
        <property type="entry name" value="G PROTEIN COUPLED RECEPTOR, CLASS C, GROUP 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511:SF15">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY C GROUP 5 MEMBER C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 12 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636665" evidence="8">
        <location>
            <begin position="26"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="9">
        <location>
            <begin position="98"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013929241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013929242.1"/>
        </source>
    </evidence>
    <sequence length="441" mass="48923" checksum="374A0730068B3CB6" modified="2020-10-07" version="1">MATSRTQLPCLWLLVLSMTTCLAQGTSPPPSPPPGCGKDLNSIYYNLCDLSVAWGVVLEAVASFGVVTSFVLTVVLVASLPFIQDYRKKSLIGTQAFFLLGTLGLFCLTFAFIVKQDFSTCVSRRFLFGVLFAICFSCLAAHSLSLNFFARQNTGPRGWVTFGMAFVLVLVEVIINVEWLIITVVRNDGTAKDPCELKNEDFVMALIYVMFLQVATFIATWPVLCGHYKHWRKHAIFILLTTSFSMAIWIVWIVMYVYGNQQDQRSTWDDPTLAIALVSNASVFVLFYVIPEISQVVKPGPEEAYEDDVYPTRGVGYETILKEQKSQSMFVENKAFSMDEPSSAKKPVSPYSGYNGQLLTSVYQPTEMTLMHSGPLDAPYDVILPRATANSQMMGSANSTLRADDAYVSQSKQALAQKDGRNGQVSEMTFPNKEPSNVRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZR7</accession>
    <name>A0A6I9YZR7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Probable C-mannosyltransferase DPY19L3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DPY19L3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930213.1">
        <property type="nucleotide sequence ID" value="XM_014074738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZR7"/>
    <dbReference type="GeneID" id="106555824"/>
    <dbReference type="KEGG" id="tsr:106555824"/>
    <dbReference type="CTD" id="147991"/>
    <dbReference type="OrthoDB" id="6019623at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018732">
        <property type="entry name" value="Dpy-19/Dpy-19-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31488:SF4">
        <property type="entry name" value="C-MANNOSYLTRANSFERASE DPY19L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31488">
        <property type="entry name" value="DPY-19-LIKE 1, LIKE (H. SAPIENS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10034">
        <property type="entry name" value="Dpy19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="482"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013930213.1"/>
        </source>
    </evidence>
    <sequence length="716" mass="82198" checksum="B486DCF984B910AE" modified="2020-10-07" version="1">MTSVRHRRGIKSTNVPQDCLSQDKNWKLNEKSVAGAKQWKKISLVIGGIVGLFLGIITSVYVSTLHENDLWFSNIKEVEREISFRTECGLYYSYYKQMLAAPSIQQGLHSLIYDNKTESMRTINILERMNVYQEVFLSMLYTVLHLKRFLEPIYFYIYTLFSLQAVYVLALYVTSWLLSGTWLSGVLAAVWYIVNRVDTTRVEFTIPLRENWALPFFAVQLVAITVLFRSHLRSVHERLMLLAVFIATFLLSLTWQFNQFILLIQAMALFTLDCLDMIPEGKVIRLYIVQVASLLMVYLLQFFNSMIIGSLLVSFNMSALLARKLQNNLKTGGLLSRIGKLLLHVLLVLCLTFLVNNFIKRILNLQSDEHIFKFLKAKFGFGATRDFDANLYLCEEAFGLLPFNTFARLSNTLVLYPYLFVLLITATTAAVVSFQNLSGRASIKSAGKQPTSATVFKPAMAYHLVHTISFGLLALSTMRMKYLWTSHVCMFAASGLCSGDIWGLVLKCVHLHTPKRVSVIRYVTPILVLLYLCYKFLPGIMAELSELREFYDPDTVELMNWIKSNTPKKAVIAGSMQLLAGVKLCTGRILTNHPHYEDKSLRERTKQVYQVYAKRSPEDVHRILRSFGTDFVILEDSICYERRHSRGCRLRDLLDIANGHTMDGPGEYDPDLKPSPSPRFCEEIKRNLPSYTMYFTKVFHNKTFHVYKLARHQNHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZY0</accession>
    <name>A0A6I9YZY0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Neurabin-I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106555171</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929426.1">
        <property type="nucleotide sequence ID" value="XM_014073951.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555171"/>
    <dbReference type="KEGG" id="tsr:106555171"/>
    <dbReference type="OrthoDB" id="62701at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09512">
        <property type="entry name" value="SAM_Neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000008">
        <property type="entry name" value="Neurabin-1 isoform 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040645">
        <property type="entry name" value="Neurabin-1/2_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043446">
        <property type="entry name" value="Neurabin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154">
        <property type="entry name" value="NEURABIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154:SF26">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 9 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17817">
        <property type="entry name" value="PDZ_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="578"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="18">
        <location>
            <begin position="1222"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="182"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="325"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="952"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="751"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1272"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="964"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1006"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1020"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1043"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1070"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1145"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1192"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013929426.1"/>
        </source>
    </evidence>
    <sequence length="1317" mass="148167" checksum="1C0D652D3DC4945D" modified="2020-10-07" version="1">MMKTEGKGDRTLRSASPHRNAYKTDFHAIKCSFDSVTTDNASNKSNPQQKLTNSSNGEGNDPHVRGRAVSYGNRVHKIKNMFMQMGGSSNSPCCENSPPSEPRIINKITSADLALPPPSSPSYLCVQKNNLLNSGSFGSSPDPLPACSFTDKIICSKEEGNLDKATLAEKFSETRKLFERNIKKPSSLDRSPPIKGERIEDRRRHGSTSSDGSSVTDHFNFNIDVTSQVAVDKTENQGNSDQKGQQSTGSCKSSSLNAGPISRRLESFLADIDNDDFKDTLKMKDIPNPVQTVFCGWESPGVRKEVYLQQNQPIVESSLLISSTSTERSELSDTTEVTSQTDQTAEREQQYKPSISSKVDRAPIKISQVEVARAELIMVQNKTLGDNNEITGEVNVFQKEKPLRFPSWKEEKNDCTFEEMEESNKDGAVNQKGARELAFLDKIQEQEQVHVKPVGDGEEEEQEQRKKLSGFSSSALGIENAAFDDDRETELISQGPETEETLQEEEYNYNSDYEELSGLSEEDDPDPARRVKFSTAPIKVFSTYSNEDYDRRNEEVDPVAASAEYELEKRVEKMEVFPVEIEKGDNGLGISIIGMGVGADQGLEKLGIFVKTVTEGGAAQEDGRIQVNDQIVEVDGISLVGVTQFFAATVLKNTKGSVRFLIGREKPGTQSEVARLISETLEQERYLYEQQYAHETEQDEDYDEDDVSFESHLQEKSVEVFYLPENEDITFPIDMDSAQFMLKFKELQLKHAVTVAEVNQLKEKLKATEAEKIEWELKKSQLQETIDDNKEKIKKLETYWLEAQSLCKTVNEHLKETQEQYDVLEKKYNKAKKLLKEYQQKEELAIKEDHTKIIEENNQEHANQLKFLQEKITELEDKLKIYEKLPYLFGNSMLLDSVTPNTEQLAEHFEIKDDMQELESSVEKLDFSDFDTFVGDIPRLDTSAHKAKAQLALKVKRQPPSRSKLKESLGAKQKDRISQDINDDTEENTLSKFPDVTEKVISSQQRDTDKDDKLECAENSDLSLEPSPPLSVHSSPVYKLQTEETAGISQPVPNDDSTPNSPAGYCRNVKQRESKGKGKVNKDDKRGEEKAESGDGPSVGKSKRRFPDFGGLRKSGGKGKKQEKENARGSLDSRGSRELLDDPDNNLSASDSDSPIPTCMPFSWFGDSHKEHSSSSTLSFSQGGQDIAIEQSQEKTRSKAIDDEPSATGKQNQWHNRPISEWTTQQVCHWLMGMNMEQYISEFTTKNVDGQQLMLLDSEKLKALGVSSQNDRSTIKKKIKDIKKTQEKLEKQKEKFQKKEKEVRKTGKLVAAVESNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z060</accession>
    <name>A0A6I9Z060_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Semaphorin-4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SEMA4A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929674.1">
        <property type="nucleotide sequence ID" value="XM_014074199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z060"/>
    <dbReference type="GeneID" id="106555365"/>
    <dbReference type="KEGG" id="tsr:106555365"/>
    <dbReference type="CTD" id="64218"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF15">
        <property type="entry name" value="SEMAPHORIN-4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="452"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="9">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="489"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929674.1"/>
        </source>
    </evidence>
    <sequence length="565" mass="62163" checksum="86960C38FBEA9E08" modified="2020-10-07" version="1">DAAFVASFNVPGPPGEEKIYFFFEETAKEFDFMEKLTVSRVARVCKNDVGGDKVLQKKWTTFLKAQLSCSQAGFFPHTVLKHVFALPQPRGGFLFYGIFASQWQNGNSGSSAVCAFSLDAIQKAFDGKYKELNKDCSRWTTYSGPAMDPRPGSCLVGPSSDKALTFMKDHFLMDEKVAPVHDQPLLVKEDVKYTRIAVHQAQKPCGAACTVLFLGTDRGSLHKAVVVGHRAHIIEEIQLFEKPEAVQNLLLVPEKGVLYVGYSEGILEVLVANCSVYATCASCLLARDPYCAWDGRLCRAVWEDTRNRTDWLQDVEQGKAEATCQRQSGKGRAMPKSRNDSDGVPLKVLSGPFNSMMRLSCPPVSALANYSWTYPEGSLPSQEGLTVRDHTGLLVFVHSRTLGEYACWASENGYRHRVAWYDVRSDLPMGGASSANGSPEHQAVPVGEWRSYWVQFVTVTVLLSMTLAVAMALGFFSYHDKLKAKTKVQGCSVPEPSRTPVQEKAPLNGSQSPPGDRNYQSQEATDGTKSCCVQVEGGSRAMDTDNNRLNCPLMNGEEARAVEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0A3</accession>
    <name>A0A6I9Z0A3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Scavenger receptor class F member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SCARF2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930418.1">
        <property type="nucleotide sequence ID" value="XM_014074943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0A3"/>
    <dbReference type="GeneID" id="106555994"/>
    <dbReference type="KEGG" id="tsr:106555994"/>
    <dbReference type="CTD" id="91179"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000041">
        <property type="entry name" value="Tyrosine protein kinase receptor tie-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042635">
        <property type="entry name" value="MEGF10/SREC1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043:SF5">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="465"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="789"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="808"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="823"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013930418.1"/>
        </source>
    </evidence>
    <sequence length="837" mass="90073" checksum="49A6ECDF2FF9E2D1" modified="2020-10-07" version="1">MGSETPQGAHLLLPSLAECPRLFWGPDCKELCSCYPNGQCEDVTGQCTCNANRWGPRCENVCLCKHGKCDQKTGKCSCEPNWWGPQCASACYCSLNSQCDQKTGLCLCQPGWWGRTCNNQCACSTSPCEQFTGRCQCRSRTFGPRCDRYCQCHMGKCNQVDGTCTCEPGYRGKFCREACPAGFYGQGCRRRCGQCKELQPCTIVDGRCLACEVGWNGTKCDQICSLGFYGEGCEQICPPCKDGHTCNHINGKCSHCNPGWIGDRCQTKCRNGTYGENCAFVCSSCFNGECHFETGRCLCQAGFHGTFCNLTCPTGHYGANCTELCNCHDDTCNPFTGMCHMEANQRMGLIGAGVLLTFLLCLVLSLLSCCCICRKKDQARDIRQDLAHKKSSRRLCGQFSQISLKLPRIPLRRQKLPKVVVAHHDLENTLNCSFIEPPSVVDQPSPSWSSRGSFSSFDTTDEGPVYCVPHEESDHKERVSAPSSTLEKAPQPLRKGPAVAAEEEEAGEYTVLKEGPADGCEAPLLKSSDSERSSCGSGSVSGALYARIARLSKQSREEEEAPAAEPRPPKPPSPERAKPPPPDPSTKPKVSWIHHKYNAAQSNSLPAAAELQAERRPGPSKRKRSPGEAARAKERAPKPPQEPGSPEGQPGLAQARQRSRTGPEPAENLNGSLHSALKRMGAPAAAERRPAEALRSPAQPKAPPGFSEAATLLAAQLKEKTQSLQRGETAARPNGVSPLPAQREKPAPPHKAKQSAAPAGQKAGKPLLPTSAPLQKLLGPEAKSAEKQSAGGSPEATPAPGEPAAKKTPIKKPPRKKGREGAAEPPKAAASSPQTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0B0</accession>
    <name>A0A6I9Z0B0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 420-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106555408</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929729.1">
        <property type="nucleotide sequence ID" value="XM_014074254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0B0"/>
    <dbReference type="GeneID" id="106555408"/>
    <dbReference type="KEGG" id="tsr:106555408"/>
    <dbReference type="OrthoDB" id="9043260at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000739">
        <property type="entry name" value="Zgc:171418 protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000706">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000038">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002604">
        <property type="entry name" value="Zinc finger protein 715"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000575">
        <property type="entry name" value="Zinc finger protein OZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF41">
        <property type="entry name" value="ZINC FINGER PROTEIN 613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="500"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="528"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="584"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="612"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="640"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="668"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="696"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929729.1"/>
        </source>
    </evidence>
    <sequence length="730" mass="84712" checksum="CF78263D0A596E17" modified="2020-10-07" version="1">MEGGKLWDPPSDTSSTPKDHKRTHECSECGKFFSLHSLLLTHRKVHVGSGSSQGSEGEKSFSGKNSKDLRSPPTLKPYKCEECDLCFRQKSYLRAHQKIHTGEKPHQCLECGKFFRKKAYLRRHESIHIEEKPYKCWECGKSYSQESCLWRHKKIHLGIKPYKCFECGNGFTRGSSLRSHLKIHTGEKNEKCHECGKCFIQKSTLMRHQRRHTGEKPYECPECERRFVDSSDLRRHQKNHMGEKIFKCGKCGKSYLTQGEIQVHERSHTREKAFSCEECGKCFFRKHHLARHHKTHTGEKPYQCNKCGSFYISISAFRSHVENTSNTPSNQGTQEHPEGWISFPLCCIVLTHQNVHVGSGSSQGLENVESSSGKEAGGLRSPPEPKPYKCEECDLPFDQMSKLLRHQRIHTGEKPYQCLECGKRFRGKGNLRSHERIHTGEKPYKCWECEKSFCQEASLRAHEKIHLGIKSYKCFDCGKRFTLRQSLYSHEKRHKGEKNEKCLECGKCFIFKSSLVQHQRRHTGERPYECEECERRFVDHSEFRIHQRRHKGEKPYECGECGKSFLTRTEIRNHETIHTGEKPFKCGECGKCFSLKQQLKTHQRIHTGEKPYRCVECGRCFRHVGDLSIHHRTHTGEKPYQCNECGKFYSTSSALGTHMKIHTGEKNFKCLECGKAFIQSGSLIRHSRIHTGEKPYKCSECNKCFSQKVALLKHQRIHTAEQPHNEDEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0H2</accession>
    <name>A0A6I9Z0H2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106555705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930072.1">
        <property type="nucleotide sequence ID" value="XM_014074597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0H2"/>
    <dbReference type="GeneID" id="106555705"/>
    <dbReference type="KEGG" id="tsr:106555705"/>
    <dbReference type="OrthoDB" id="6251307at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF147">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930072.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15595" checksum="68C4A387F1499F71" modified="2020-10-07" version="1">MLEGLILKQSKGFHVEFAYGQFKGANIPLKFKLHFERTPASSNSPILTFNSFVNQQWGKEIRMATHLQPAQKFKLSFVVTSKSYKIMENDILLSEFDHRISPKAIRFLGMDGDIKLEKVAFSWERNNETSNPKAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z183</accession>
    <name>A0A6I9Z183_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Angiopoietin-related protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106555691</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930059.1">
        <property type="nucleotide sequence ID" value="XM_014074584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z183"/>
    <dbReference type="GeneID" id="106555691"/>
    <dbReference type="KEGG" id="tsr:106555691"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072377">
        <property type="term" value="P:blood coagulation, common pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042730">
        <property type="term" value="P:fibrinolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF7">
        <property type="entry name" value="FIBRINOGEN BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="4">
        <location>
            <begin position="50"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930059.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="23206" checksum="2B211473184017D0" modified="2020-10-07" version="1">VNTLSPLHRREKDPQEAPRWRVTALRSVLDERAANQSQAAGEQTPEGPRDFWLGLEKMHGMVKDDRFQLLVELRDWEGNAQRAHFLFRLGGEETAYTLSLLGPIRGELESAMGDFPQLPFSTRDRDHDLKGDTNCAKHLSGGWWFSYCGHVNLNGKYFRSIPRQRHERKQGIFWKTWRGRYYPLQSATMKIRPAEPDRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1N6</accession>
    <name>A0A6I9Z1N6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transmembrane protein 104</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMEM104</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TMEM104 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929966.1">
        <property type="nucleotide sequence ID" value="XM_014074491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1N6"/>
    <dbReference type="GeneID" id="106555611"/>
    <dbReference type="KEGG" id="tsr:106555611"/>
    <dbReference type="CTD" id="54868"/>
    <dbReference type="OrthoDB" id="294541at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="19"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="134"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929966.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="56313" checksum="A60DF57F91D360D8" modified="2020-10-07" version="1">MAGSITDTGELYSPYVGLIYMFNLIVGTGALTMPKAFAAAGWLVSLILIMFLGFMSYMTTTFVMEAMAAANAQLHLKRMEKHKEDDNDDDSSSEVSDSEVLPDVYERSETRPILSVQRRGAPNIFEITERVEMGQMASMFFNKVGVNLFYFCIIIYLYGDLAIYAAAVPVSLMEVTCTVTGNHSCSVEDGTKYNDTDKCWGPIRRIDAYRIYLAVFTLLLGPFTFFNVQKTKYLQIMTSLMRWLAFIIMIIVALLRIAKGQEEGHPPLAQLSGVRNLFGVCVYSFMCQHSLPSLITPISKKKHVNKLVLLDYVLILGFYSLLSFTAIYCFRNGTLMDMYTLNFTNCDIVSIAFIRYFLGLFPVFTISTNFPIIAVTLRNNWKTLFHREGGTYPWVVDRIFFPAITLIPPIIVAFCTHDLESLVGITGAYAGNGIQYIIPAFLAYYSRKETQTTFRNRALNKHLSPFRHTFWIGFVLLWGLFCFLFVTANIILSETKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4S5</accession>
    <name>A0A6I9Z4S5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">RNA-binding protein MEX3B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MEX3B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930865.1">
        <property type="nucleotide sequence ID" value="XM_014075390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4S5"/>
    <dbReference type="GeneID" id="106556394"/>
    <dbReference type="KEGG" id="tsr:106556394"/>
    <dbReference type="CTD" id="84206"/>
    <dbReference type="OrthoDB" id="427410at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22424">
        <property type="entry name" value="KH-I_MEX3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000013">
        <property type="entry name" value="Mex-3 RNA-binding family member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000090">
        <property type="entry name" value="Mex-3 RNA-binding family member C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000012">
        <property type="entry name" value="Mex-3 RNA-binding family member D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047226">
        <property type="entry name" value="KH-I_MEX3_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047227">
        <property type="entry name" value="MEX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285">
        <property type="entry name" value="RING FINGER AND KH DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN MEX3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="429"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
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        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013930865.1"/>
        </source>
    </evidence>
    <sequence length="480" mass="49940" checksum="A08CE37A79D4024C" modified="2020-10-07" version="1">PPPSAPGCKIKALRAKTNTYIKTPVRGEEPLFVVTGRKEDVAMARREIISAAEHFSMIRASRNKNTVLNGSVPSPPNLPGQTTIQVRVPYRVVGLVVGPKGATIKRIQQQTHTYIVTPSRDKEPPVFEVTGMPENVDRAREEIEAHIAMRTGGLIELIDENDFHANGTDVGFELGGGGAGSLWSKPTPSITPTPARKPFCSYRNDSSSSLGSASTDSYFGSSGRLADYSPPSPAALGFPPNGGPSNGYGVYGTPAEALASPDGCPAELPPFDSPPGFDLAPAPPAQLLWSQYERSTSLASPAIAAAAAAFPGSATTAAAPALFVSGGQRRGVPQAQPPTRVSPPLHSGAGGGSLAASEQHPLARRVRSDPGGRFLAYPLYANGLGCHLPGLPSNCSPSSSSSSSSSASSSSSSSSCSSSGLRRKGSRDCSVCFESEVIAALVPCGHNLFCMECANRICEKNEPQCPVCHSAVTQAIRIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z588</accession>
    <name>A0A6I9Z588_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Laminin subunit alpha-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106556679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931131.1">
        <property type="nucleotide sequence ID" value="XM_014075656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z588"/>
    <dbReference type="GeneID" id="106556679"/>
    <dbReference type="KEGG" id="tsr:106556679"/>
    <dbReference type="OrthoDB" id="5836593at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:regulation of cell adhesion"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045995">
        <property type="term" value="P:regulation of embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009254">
        <property type="entry name" value="Laminin_aI"/>
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    <dbReference type="Pfam" id="PF06008">
        <property type="entry name" value="Laminin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Laminin alpha" evidence="2">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
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            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
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        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013931131.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="27667" checksum="813490F0737925FE" modified="2020-10-07" version="1">MLKDIRLRRPFNFQRQLAVEESEAAQDLLRKAESWEQKHNETRSLIPDILDQLGEHNTKLTDFQEALHQVIDDIRKTATTNNESIAKLQVHEKQQEILEEDIDTLNNTLGMATDILDVSHGIIFEVNEAIQNASRFYAEIDGAKVQLQDKQANQSRFDEDLVEKAMKYAQGLQDLADNLERTFHGIDTNGLVQKALNASNVHENIINYINEANQMSVLALNTTERVSDAAVGIDTQITYHKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z6G8</accession>
    <name>A0A6I9Z6G8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Cation channel sperm-associated auxiliary subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Transmembrane protein 146</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">LOC106557138</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">Auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for CATSPER1 stability before intraflagellar transport and/or incorporation of the CatSper complex channel into the flagellar membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Cell projection</location>
            <location evidence="21">Cilium</location>
            <location evidence="21">Flagellum membrane</location>
            <topology evidence="21">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CATSPERD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931764.1">
        <property type="nucleotide sequence ID" value="XM_014076289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z6G8"/>
    <dbReference type="GeneID" id="106557138"/>
    <dbReference type="KEGG" id="tsr:106557138"/>
    <dbReference type="OrthoDB" id="8646292at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036128">
        <property type="term" value="C:CatSper complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097228">
        <property type="term" value="C:sperm principal piece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048240">
        <property type="term" value="P:sperm capacitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028751">
        <property type="entry name" value="CATSPERD/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053814">
        <property type="entry name" value="CATSPERD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053813">
        <property type="entry name" value="CATSPERD_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055451">
        <property type="entry name" value="Ig-like_CATSPERD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33722:SF1">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED AUXILIARY SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33722">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED PROTEIN SUBUNIT DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15020">
        <property type="entry name" value="Beta-prop_CATSPERD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22850">
        <property type="entry name" value="CATSPERD-E_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23747">
        <property type="entry name" value="Ig-like_CATSPERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="16" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0282">Flagellum</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="654"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="27">
        <location>
            <begin position="34"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERD beta-propeller" evidence="26">
        <location>
            <begin position="113"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERD beta-propeller" evidence="26">
        <location>
            <begin position="226"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERD Ig-like" evidence="29">
        <location>
            <begin position="315"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERD/E C-terminal" evidence="28">
        <location>
            <begin position="462"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00040129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00041424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00046028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF15020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF22850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF23747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013931764.1"/>
        </source>
    </evidence>
    <sequence length="683" mass="78160" checksum="FFC47DE715F7D19D" modified="2020-10-07" version="1">MTRLRAVLHHFNQAGLKVKHEKCQIVVPQVEFLGTLSAAERNYGQVDKEALAIVVGVKRFHHYLYGRQFQIVTDHKPLLGLLASDKQTPQMMSLRMTRWIVYLAAYNYTLVYRQALYLGERLFISFDMFDSSLLPLTIPDGFTRSPAIVSAAAFGQDTIVAVVNGAVFLYLYATWKRWLHSKGITHPVTDLASYTCCFALNDPACDKINELILAYHPGNLVSNTTTNNELALLTKTQLFYGNFDMVIRSVVHLGDKTLSSANVSCETMLFENIGILSIIHPIPGNASNYYHFQKCIINIQEKLMTVKPLPRHCPMEILTGDFHNRMYYIDTKQQLHFNATFVPKPGTSAYPFVMVTNPGVLVFEAHIMEDGYTFNGNPKYSLKIKLEEQPQLTSMMVGKQNSTLLKLSSITVDIYNKGLFCIDMYPLTALIAVDCPPKKHIRILRTTTGCSKGLFEPRLLQNFVYSIDKNLYDPLFLGRKNLAQDNLNVTYQYDTWGCPILLYYDKPWLPKLELWNDDEFVEYVSADFVLYEINGMHNYDYLLTEIEANCLSEAQNWTKQLAIFPGSPHIAWTRYTYESCKNPKGNTSLPSIKSKYQVLNRDEGNRILFPQYNGFYVFRAIVVDRLYSYCDFTTVFSVYVHGALPKSKVSVGKALISFLVLIFGTMLMVYFFLKLLKEYSRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z794</accession>
    <name>A0A6I9Z794_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FH2 domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106557094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931695.1">
        <property type="nucleotide sequence ID" value="XM_014076220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z794"/>
    <dbReference type="GeneID" id="106557094"/>
    <dbReference type="KEGG" id="tsr:106557094"/>
    <dbReference type="OrthoDB" id="26518at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF11">
        <property type="entry name" value="FORMIN-J-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="555"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="627"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="278"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="560"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="664"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="833"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931695.1"/>
        </source>
    </evidence>
    <sequence length="941" mass="105185" checksum="87F717E462B1645E" modified="2020-10-07" version="1">MNIGIFLKQFKKSLDSLIEDLHTGRDFYSSETLRELLKLLPEAEEVKKLKDFSGDLSKLCQADSFMYMLIQVPNYSLRIEAMVLKKEFSPSCTSLQKNMTIIKMATKELMCCEELHAILHLVLQAGNIMNAGGFAGNAVGFKLSSLLKLADTKANRPGMNLLHFVALEAQKKDKVLLTFSEKLHHVHEAARISLENIETELHSLSSKTKSLKDSIRRDTELFRQMEGFLQFAVKELKELDHWKRELMKEAHALIDFLCEDKETMKLEECFQIFRDFCLKFNKAVKENKEREVQQLQQLQRLKELEDKRRSWVAADISAFGRSSSENDVGALTKRGLEEFLPFLQQRPQSPSYRNTSTRRSRHSLGVTAERELFTFLSTSKGEDLNKFNSLPREHPFPTRPNVAWSENKEPVDVSFTHLHLDQVSKNKVHSYQFFPSQAQHVEDASHDNLYSNQERSNNDKYGFNVPCVESGDAVPWDLSMEEHEHIPRLHRFDSHETNDVEDSSPVHFVAGMQGLDAQDGPLHSPGITGDPSNREKTHYTIEHSAKTMSYVNEALEPDNDSPISSDGSISGTKTQGPVFFESDFECSLTLDLSEDQSSKLGGNKIDNKNGANSSYMNELQTGENAIFSNDKSDCSNKSSFPKEKSVRSKDSLGPKRNSLKDKTSGTTKPSTASPNHPRPVRTLNSSENASMRKVVPISRSTKPASPSCPKKPEAKPASRDTAVAETRLSRRNSVRGIVDAAPKPPYRQSISVEEPKFQHGTSNSSSGHCEKEQLQHKGSFKKPSCKPVRYVPKSKTDETKMCRSLNKSQGPVDANKSTSATAPKAPAPVPSFARNTVASSSKCAKVDLPPPSRAPPLTRSLSQRLPRMKVTAASDDFNPKENGGTLKRANSAKIIKRNTDNSDLLSVKADSAPGEQIMEKSGSFKCKDGSQTTIGKLFNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WWC2</accession>
    <name>A0A6I9WWC2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enhancer of polycomb homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">EPC1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907209.1">
        <property type="nucleotide sequence ID" value="XM_014051734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WWC2"/>
    <dbReference type="GeneID" id="106537563"/>
    <dbReference type="KEGG" id="tsr:106537563"/>
    <dbReference type="CTD" id="80314"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009607">
        <property type="entry name" value="Enhancer_polycomb_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06752">
        <property type="entry name" value="E_Pc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Enhancer of polycomb C-terminal" evidence="9">
        <location>
            <begin position="580"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013907209.1"/>
        </source>
    </evidence>
    <sequence length="616" mass="71220" checksum="8604092DF6882ECA" modified="2020-10-07" version="1">MSKLSFRARALDASKPLPVFRCEDLPDLAEYASINRAVPQMPTGMEKEEESEHHLQRAISAQQVYGEKRDNMVIPVPEAESNIAYYESIYPGEFKMPKQLIHIQPFSLDVEQPDYDLDSEDEAFVNKLRKKIDISPLQFEEMIVQLEKGSGQQPVSLQEAKLLLKEDDELIREIYQYWIKKRKNCQGLISVVKQEKRDGSSTNDPYVAFRRRTEKMQTRKNRKNDETSYEKMLKLRRDLSRAVTILEMIKRREKSKRELLHLTLEIMEKRYKLGDYNGEIMSEVMVHRQPMKPTYTIPVGIVPNSTQFKHQENIELKEFKINKQEKAEAIRPKRKYEKKPKIVPSSAAIASQQTVPASLPIFNAKDMNQYDFPSSDEDPLSQIFSGSSETEEENDPDGPFAFRRKTGCQYNAPHLDQTGDWPWNTPKGGTLGDERYRYCLTTLTRPQKCIGFSRRRVGRGGRVLLDRAYSENNSAFCQLDSETPSSQLHSSVNQIANTSETNTSDRYLSKDLSQILVDIKSRRWRHFRPRTPSLHDGDNGELSYRKLHRSVNRAGTAQPGNQTCSTSNQSKDSSGAAHFAFTAEQYQHHQQQLALMQKQQLRNQGNKDYDNSYRWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0Y1</accession>
    <name>A0A6I9X0Y1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyridoxal kinase</fullName>
            <ecNumber evidence="9">2.7.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Pyridoxine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28 29 30 31" type="primary">PDXK</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29 30" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28 29 30">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively. PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>pyridoxal + ATP = pyridoxal 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17310"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:10225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>pyridoxamine + ATP = pyridoxamine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57761"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:25105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>pyridoxine + ATP = pyridoxine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25108"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16709"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:25109"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxal: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxine 5'-phosphate from pyridoxine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="9"/>
    <dbReference type="RefSeq" id="XP_013907457.1">
        <property type="nucleotide sequence ID" value="XM_014051982.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907458.1">
        <property type="nucleotide sequence ID" value="XM_014051983.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907459.1">
        <property type="nucleotide sequence ID" value="XM_014051984.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907460.1">
        <property type="nucleotide sequence ID" value="XM_014051985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0Y1"/>
    <dbReference type="GeneID" id="106537755"/>
    <dbReference type="KEGG" id="tsr:106537755"/>
    <dbReference type="CTD" id="8566"/>
    <dbReference type="OrthoDB" id="2104723at2759"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00298"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000007">
        <property type="entry name" value="Pyridoxal kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0007">Acetylation</keyword>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16 28 29" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="26">
        <location>
            <begin position="96"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013907457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013907458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_013907459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_013907460.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="35123" checksum="6E6C16D77BEA0A94" modified="2020-10-07" version="1">MEEEEECRVLSIQSHVVRGYVGNKAATFPLQVLGFEIDTVNSVQFSNHTGYNHWKGHVLNSNELHELYEGLKLNNVNHYDYVLTGYTRDKSFLAMVVDIVQELKQQNSNLVYVCDPVMGDKWSGEGCMYVPKDLFPVYKDNVVPVADIITPNQFEAELLTGRKIHTEKEAIEVMDMLHNMGPETVVITSSDLQAPSGNDYLIALGSQRRTTADGTIVTQRIRIESPKVDAVFVGTGDLFAAMLLAWTHKHPDNFKMACEKTVSAMQHVLQRTIKSAKAEAGEGNKPNSAQLELRMVQSKKDIENPEIIITATVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2V5</accession>
    <name>A0A6I9X2V5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein chibby homolog 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CBY3</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908651.1">
        <property type="nucleotide sequence ID" value="XM_014053176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2V5"/>
    <dbReference type="GeneID" id="106538633"/>
    <dbReference type="KEGG" id="tsr:106538633"/>
    <dbReference type="CTD" id="646019"/>
    <dbReference type="OrthoDB" id="2145765at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd07429">
        <property type="entry name" value="Cby_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028118">
        <property type="entry name" value="Chibby_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21533">
        <property type="entry name" value="LEUCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21533:SF17">
        <property type="entry name" value="PROTEIN CHIBBY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14645">
        <property type="entry name" value="Chibby"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013908651.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="17960" checksum="F6227CC60CC4F7A4" modified="2020-10-07" version="1">MRDYWADHFSRKFYPRRPTLRQATTLSTFYLLDYKTRQAELGMNYDHPCAQLNKAKFVFHNGEWKNENIPQQMPLLQLFPSPEKESPNKALKKANKSLLEENNYLKLQLELLMDMLTETTARLHLVEKKLDTTTWHSNMKKSNNKVLMLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X345</accession>
    <name>A0A6I9X345_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BarH-like 2 homeobox protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">BARHL2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the BAR homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909714.1">
        <property type="nucleotide sequence ID" value="XM_014054239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X345"/>
    <dbReference type="GeneID" id="106539443"/>
    <dbReference type="KEGG" id="tsr:106539443"/>
    <dbReference type="CTD" id="343472"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000097">
        <property type="entry name" value="barH-like 2 homeobox protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF4">
        <property type="entry name" value="BARH-LIKE 2 HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="217"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="219"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="146"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013909714.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="41016" checksum="B9E6AD12D4766D47" modified="2020-10-07" version="1">MEGSSTSSFGIDTILSNPRSGSPVMMNGDFRTAHGEARAADFLNQATPSPCSEIDTVGTTPSSPISVTAEHPEQHLAQDGLSPQHHHLHHGQHSEQQQPKSLQPSPQQQQQQQQLGSCSSVPRTSTSSFLIKDILGDSKPLAACAPYSTSVSSPHPTPKQESNSANESFRPKLEQDDSRTKLDKRDDSQNELKCHGAKEEGDREISSSRDSPPVRAKKPRKARTAFSDHQLNQLERSFERQKYLSVQDRMDLAAALNLTDTQVKTWYQNRRTKWKRQTAVGLELLAEAGNYSALQRMFPSPYFYHPSLLGSMDSTTAAAAAAAMYSSMYRTPPAPHPQLQRPLVPRVLIHGLGPGGQPALNPLSNPLPGTPHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X3P8</accession>
    <name>A0A6I9X3P8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell division cycle protein 20 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">p55CDC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CDC20</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Substrate-specific adapter of the anaphase promoting complex/cyclosome (APC/C) complex that confers substrate specificity by binding to substrates and targeting them to the APC/C complex for ubiquitination and degradation. Recognizes and binds the destruction box (D box) on protein substrates. Involved in the metaphase/anaphase transition of cell cycle. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with NEUROD2. Interacts with dimeric MAD2L1 in its closed conformation form. Interacts with BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with SIRT2. Interacts with isoform 1 of NEK2. Interacts with HSF1 (via phosphorylated form); this interaction occurs in mitosis in a MAD2L1-dependent manner and prevents PLK1-stimulated degradation of HSF1 by blocking the recruitment of the SCF(BTRC) ubiquitin ligase complex. Interacts (via the N-terminal substrate-binding domain) with FBXO5. Interacts with CCNF. Interacts with USP22.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908926.1">
        <property type="nucleotide sequence ID" value="XM_014053451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X3P8"/>
    <dbReference type="GeneID" id="106538824"/>
    <dbReference type="KEGG" id="tsr:106538824"/>
    <dbReference type="CTD" id="991"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000224">
        <property type="entry name" value="cell division cycle protein 20 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF3">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 17" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 13" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="15">
        <location>
            <begin position="200"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="329"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="376"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="464"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="42"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013908926.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="57698" checksum="E9D7234573D38ACE" modified="2020-10-07" version="1">MAEGAGRVGVRAVHCVPMAQFLFESDLHGLLKLDAPIPNVPPARWQRKAKDAAAPSTGAASDAIAPSPMKPANRPHSTSKTPSKTPGKSGSKSQNTPNKTGGDRYIPNRSAMQMDVANFLLTKEESSEETPTKKEHQKAWAMNLNGFDVEEAKILRLSGKPQNAPEGYQNNLKVLYSQKSTPGSTRKVNRYIASVSERILDAPDIRNDYYLNLVDWSSLNFLAVALDKSVYLWNYDSREIIQLMQMENPDDYISSVSWIREGNYLAIGTSNAEVQLWDIQQKKRLRNMVSHSSRVSSLSWNNYILSSGSRTGHIHHHDVRVAEHHVATLAGHTQEVCGLKWAPDGRYLASGGNDNLVNIWSVAQGDSWSPRPVQTFTQHQGAVKAVAWSPWQSNVLATGGGTSDRHIRIWNVCSGSCLNTVDAQSQVCAVLWSTNYKELVSGHGFAQNQLVVWKYPSMSKVTELKGHTARVLSLTMSPDGTTVASAAADETLRLWRCFELDPARKKEKESSTKTSIIHQGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X4T0</accession>
    <name>A0A6I9X4T0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 68-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106538782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013908867.1">
        <property type="nucleotide sequence ID" value="XM_014053392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X4T0"/>
    <dbReference type="GeneID" id="106538782"/>
    <dbReference type="KEGG" id="tsr:106538782"/>
    <dbReference type="OrthoDB" id="44277at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753:SF23">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="1">
        <location>
            <begin position="41"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013908867.1"/>
        </source>
    </evidence>
    <sequence length="281" mass="31626" checksum="A9E4156E9C7ABB22" modified="2020-10-07" version="1">MYGGSNYGDQRGMASLGCCRFCDPSRHTTTSSIEPGRTGYEVIGMENLPQGPGLIVYYHGAVVFDYIMLIAKVYAKTGRTIQSVVHCFFYLIPGIKLCLDIVGCIEGKQTFCADVLNRGYLLGIAPGGLREQNFSNENYNLEWSTRTGFAKVALKNKVCSVIYGTFCYYGTFIVFHCFNATDSSCLTIHHFITVQSCDSHGLSKWLYEHFRFLCLPIYGGFPVKLRTYIGEPIPYDPNLTAKELAEKTKIAIENLRDRHQKLPGNILRALLERFDKNKKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X513</accession>
    <name>A0A6I9X513_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.16</ecNumber>
            <ecNumber evidence="10">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DUSP1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 17">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="EC" id="3.1.3.48" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013910479.1">
        <property type="nucleotide sequence ID" value="XM_014055004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X513"/>
    <dbReference type="GeneID" id="106540028"/>
    <dbReference type="KEGG" id="tsr:106540028"/>
    <dbReference type="CTD" id="1843"/>
    <dbReference type="OrthoDB" id="165342at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903753">
        <property type="term" value="P:negative regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14638">
        <property type="entry name" value="DSP_DUSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000015">
        <property type="entry name" value="Dual specificity phosphatase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000026">
        <property type="entry name" value="Dual specificity protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF309">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="14">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="175"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="240"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013910479.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="40611" checksum="9AE2C3072EFB8CBF" modified="2020-10-07" version="1">MVNMQVCALDCETLRDLLQDRDLQCLVLDCRSFFAFNSSHVRGSCNVRLSTIVRRRAKGAVGLEHILPNEEARACLHKRLYHAVVLLDERSADLEAPKRDSTLQLALNTLCREARHTRICFLKGGYESFSSTFPELCTKPSAPKGLTLPLSTTIAPGSADSNCSSCGTPLYDQGGPVEILPFLYLGSAYHASRKDMLDTLGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISCWFNEAIDFIDSIKNDGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPNCSAEAGSPAMSALDRGTSTTTVFNFPVSIPVHPSSNALNYLQSPITTSPSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X514</accession>
    <name>A0A6I9X514_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MAPK13</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013909346.1">
        <property type="nucleotide sequence ID" value="XM_014053871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X514"/>
    <dbReference type="GeneID" id="106539167"/>
    <dbReference type="KEGG" id="tsr:106539167"/>
    <dbReference type="CTD" id="5603"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000170">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000769">
        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008352">
        <property type="entry name" value="MAPK_HOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01773">
        <property type="entry name" value="P38MAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0346">Stress response</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013909346.1"/>
        </source>
    </evidence>
    <sequence length="365" mass="41999" checksum="DEF823475FDE0E67" modified="2020-10-07" version="1">MSCTRRKGFFRQEVNKIIWELPRRYTAIIPVGSGAYGSVCSAIDKKTGDKVAIKKLCRPFQSEIFAKRAYRELTLLKHMQHENVIGLLDVFTSATSFDGFQDFYLVMPYMQTDLQKIMGHQFSEEKIQYLIYQVLKGLKYIHSAGIIHRDLKPGNLAVNEDCELKILDFGLARHTDTEMTGYVVTRWYRAPEVILNWMHYNQTVDIWSVGCIMAEMLTGKTLFKGKDYLDQLTQILKVTGSPGEEFVQKLEDKAAKNYIQTLPKIPKMDLSLLLPKASPLAVDLLDKMLQLDVEKRLTATQALAHSYFDPFRDIEEETEAQHSYDDSTGSTKLSINEWRRHIYSEILTFSPIARKDSKRRSGMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X527</accession>
    <name>A0A6I9X527_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SMG1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013910494.1">
        <property type="nucleotide sequence ID" value="XM_014055019.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540041"/>
    <dbReference type="KEGG" id="tsr:106540041"/>
    <dbReference type="CTD" id="23049"/>
    <dbReference type="OrthoDB" id="10065496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05170">
        <property type="entry name" value="PIKKc_SMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000010">
        <property type="entry name" value="serine/threonine-protein kinase SMG1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000008">
        <property type="entry name" value="serine/threonine-protein kinase SMG1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031559">
        <property type="entry name" value="SMG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039414">
        <property type="entry name" value="SMG1_PIKKc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF71">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15785">
        <property type="entry name" value="SMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01345">
        <property type="entry name" value="Rapamycin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="14">
        <location>
            <begin position="341"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="13">
        <location>
            <begin position="954"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="15">
        <location>
            <begin position="2456"/>
            <end position="2488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2395"/>
            <end position="2418"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="804"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013910494.1"/>
        </source>
    </evidence>
    <sequence length="2488" mass="279578" checksum="55469401984811ED" modified="2020-10-07" version="1">MVLTKPGDSSPEVINYLGNKACECYVSIADWSAVQEWQNAVHDLKKNSSGGSVNLKADFNYIKSLSSFGAGELAECTEQLELLPGEDINLLIGGAKEKIDMKKLLPNVLSPDPRELQKAIEVQLLRSSVCLATAVGYLDQDQKWQPMTENLTKYLKQTSRIALGPLRLSTLTISQSLPVLSTLQLYCSSALENTVSSRLSSEDCLIPLFSEALRSCKQHDVRPWMHALRYTMFQNQLLEKLKGQSVPVKSHLMELGLTAAKFARKRGNVALATRLLAQCSEIQLGKTTTAQDLVHHFKKQPAPIQMDEKWGPELEIEKTKLLYTAGQSTHAMEMLSSCAICFCKSAKAESAVAKSILTLAKWIQAEWKEISGQLKQVYRARQQQNLTGLSMLSRNIYTLIDLPSVNSMEEEYPRIESESTVNIGVGEPDFILGQLYRLSSAQAPEVAKSWAALASWAYRWGRKVVDNASQGGVRLLPRETSEVQNLLPENITEDDREKIYNILGQAVCRPAGIQDEDITLQIAENEDNEEDDMVDVIWRQLLSSCPWLLDLEENSTEGLIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVPLDEDDPRLHLRNISEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGSKFPSAIPTFLGNIQGEELLGSECDGASPPASQESLKEEAKLGLNEDQAMMQDCYSKIVEKLSAANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVQSITAAPTETPHEKWFQDNYGDAIENALEKLKNPSNPAKPGSSWIPFKEVMLNLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGSTITILPTKTKPKKLLFLGSDGKNYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKDSYQAPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLNVMKSVLEELMEATPPSLLAKELWSSCATPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETAFGVTKVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLTVIPKLDSSLGEYLHLQEQLTDIEKLQGKLLEESDYLESAEGVEHPSHTLQHRYSEHTQLQSQQRAVQEAVQVKLNEFEQWISHYQAAFSNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGAFLQQRRSVLRGCLEQLHNYATVALQYPKTVFQKHRVEQWKVWMEDLIVNMTIDHCQDLYRKYEIQYAPQPPPAVCQFISTTEITLQRYAADINSRLIRQVERLKQEAVTVPVCEEQLKEIERCIKVFLHENGEEGSLSLASVIISALCSLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLTPQDLDIPNPVEASEAIHLANGVYISLQELNSNFRQIIFPEALRCLMKGEYTLESMLNELDTLIEQITDVPLQNLVESLQSYLRNAAMGLEEETHSHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPAAWRKIDIIREARSTQTNFFDDENNRQVLEEIFFLKRLQTIKEFFKLCGTFSKTLSGINSMEDQTTVNGPVPIVNVKTLYRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKYSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLLNRPQAMTVTPPPRSAILASMKKKLHTLGQIEASIVAVQEKLAALEASIEQRLKWAGGANPALAPVLQDFDATIAERRNLVLKESQRANQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSTVSDLELRLLHRVGTNHDHPIGSSEWLVIAHKQLTQDTSAQRTAQAEKEQHIDTVCETIQNLVDSIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKLWQDNIQTVLFTLVQAMGQVRSQEHIEMLQEITPTLKELRTQSQSVYNNLVSFASPLITDTPNECSSPTSVAVCQPTFAAAVRSNTGQKTQPEIMSQNTRKLIQKNLAPSADTPPGTIAGAGKSVISSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDLDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X692</accession>
    <name>A0A6I9X692_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-amino-acid oxidase</fullName>
            <ecNumber evidence="3">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SMOX</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013910974.1">
        <property type="nucleotide sequence ID" value="XM_014055499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X692"/>
    <dbReference type="GeneID" id="106540406"/>
    <dbReference type="KEGG" id="tsr:106540406"/>
    <dbReference type="CTD" id="54498"/>
    <dbReference type="OrthoDB" id="2019015at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046208">
        <property type="term" value="P:spermine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000268">
        <property type="entry name" value="spermine oxidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.660.10:FF:000004">
        <property type="entry name" value="spermine oxidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF416">
        <property type="entry name" value="SPERMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013910974.1"/>
        </source>
    </evidence>
    <sequence length="535" mass="60020" checksum="6E3ED11962576B68" modified="2020-10-07" version="1">MQSGDTSAESTDDPLSSGPRGARQPRIVVIGAGLAGLSAAKTLLENGFTDVTVLEASDRIGGRVQSVKLADATFELGATWIHGSNGNPVYHLAEDNGLLEETTEDERSMGRISLYSRNGVAYHLTSSGQRIPKDLVEEFSDLYNEVYNLTQEFFQNGKPVNADSKNSVGIFTRDVVRKRIEADQDDPETIKKLKLAMIQQYLKVESCESSSHSMDEVSLSEFGEWTEIPGAHHIIPCGFMKIVEILSRAIPETVIHLNKPVKCIHWNQSISKEIERVADHNEEHLEENAGYSVLVECEDCEFILADHVIVTVSLGVLKKRHEEMFYPSLPDEKVLAIQKLGISTTDKIFLEFEAPFWSPECNSIQFVWEDEAEAESLTYPEELWYKKICSFDVLYPPERYGHVLSGWICGEEALIMEKYDDETVAEICTEMLRKFTGNPDIPKPRRILRSSWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTVPKQVMFSGEATHRKYYSTTHGALLSGQREAAHLTEMYQDLLSCKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X980</accession>
    <name>A0A6I9X980_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Tyrosine-protein kinase Mer</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Proto-oncogene c-Mer</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Receptor tyrosine kinase MerTK</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">MERTK</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6. Interacts with VAV1 in a phosphotyrosine-independent manner. Interacts with TIMD4; this interaction enhances TIMD4-mediated efferocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013907823.1">
        <property type="nucleotide sequence ID" value="XM_014052348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X980"/>
    <dbReference type="GeneID" id="106538003"/>
    <dbReference type="KEGG" id="tsr:106538003"/>
    <dbReference type="CTD" id="10461"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001050">
        <property type="entry name" value="MER proto-oncogene, tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000834">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase MER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000089">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000296">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000111">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF257">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE MER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 32" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026769620" description="Tyrosine-protein kinase Mer" evidence="27">
        <location>
            <begin position="25"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="511"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="29">
        <location>
            <begin position="206"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="30">
        <location>
            <begin position="295"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="30">
        <location>
            <begin position="395"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="597"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00066004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00077110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_013907823.1"/>
        </source>
    </evidence>
    <sequence length="1005" mass="111367" checksum="51D0B14CEB03544A" modified="2020-10-07" version="1">MEGSRGLPLLLLLPLPLLASTVEGLASGKGEAKEFGLTTSTKSYSHETDLVGVQPVAQSFPAWLNHTSPVGALSPPMDSRLLKINTVTSSGDHAGRLLQSIKFNPTGGTILIAEHMDIKFNCSINVPKSLVKQDFPPITLWKNGKEILNVGSHYYQFDDYELTTMKAAFSIPNAHHSDKGSYSCKLTIENEEIESDPILVLLEGLPHFIKQPQGLNVTRNEPFNLTCHAVGPPEPVEIRWHWNSNQIPKKTDISPSVLSMPGLNEAAVFHCEAHNKKGLATSSEARINIKGLPSPPMEVHVLSARAHTVLLSWVPGFDGYSPLSSCSVLVKEVDVPSNTSMMIFNTSIPPHQYLIQNLSAIKDYNIGVSCKNEIGWSSLSPWITASTTEGAPSAPPLNVTISINESSSSVMVKWIKPPASQMNGKLQSYRISYVWQTSERSKNHSFSTSKDTNFIHIPFMATNVTCIVQVAAVTNGGVGPSSDPVAIFIPSNGFISVAPSTTLESRNTDSLTIVLGFICGVVIIGLILYISVIIRKKIKEENKFGYAFSGQETDLAVNYRVKKSYGRRTVELTLGNLGVSEELQEKLHDVMIVRNNLTLGKILGEGEFGSVMEGSLSNPDGSTQKVAVKTMKLDSFSQREIEEFLREAACMKDFDHPNVIKLLGVCIEPSSQQIPKPMVILPFMKYGDLHSFLLRLRIGVGSQYVPLQILLKFMIHIALGMEYLSSKHFLHRDLAARNCMLRDDMTVCVADFGLSKKIYSGDYYRQGRIAKMPVKWIAIESLADRVYTTKSDVWAFGVTMWEIATRGMTPYPGVQNHEIYDYLLHGHRLKQPEGCLDDLYEIMYSCWRTDPVDRPTFSELQLHLEKILESLPALSEPSSVIYINTNLQEEDAKELMEDDEFPQIDTVLDPNEIIQACSQRSEPTVVTVDIHESSGMGDRYILSGVNEEHRASLIRTEEEDVSLLQSIYTQNGRQWSQASTLPVGSSLADELLFADDSLEESEILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X9B4</accession>
    <name>A0A6I9X9B4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Regucalcin</fullName>
            <ecNumber evidence="7">3.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Gluconolactonase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RGN</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucono-1,5-lactone + H2O = D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="EC" id="3.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.17" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013910342.1">
        <property type="nucleotide sequence ID" value="XM_014054867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X9B4"/>
    <dbReference type="GeneID" id="106539936"/>
    <dbReference type="KEGG" id="tsr:106539936"/>
    <dbReference type="CTD" id="9104"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000027">
        <property type="entry name" value="Regucalcin homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008367">
        <property type="entry name" value="Regucalcin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF54">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01791">
        <property type="entry name" value="REGUCALCIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="16">
        <location>
            <begin position="16"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013910342.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="33009" checksum="8B393E67071E335A" modified="2020-10-07" version="1">MSSVKVECIVPVNCKIGESPVWEEKENSLLFVDITGQKVYRWTSLTKELQSISVDAPVSTVSLRKSGGYIITRGTQFAALDWKKKSITTIIDVEKDKPNNRFNDGKVDPAGRLVAGTMSQEIRPAVVERHQGSLYTLFPDHSVVKHFDKVDISNGLDWSLDHRTFFYIDSLSYHVDAFDYDLPTGKLCNRRSIYKLEKEESIPDGMCIDTEGKLWVACYGGGRVLRIDPETGKRIQTVKLPVDNTTSCSFGGYDYSELYVTSAGLDESSLIRQPQAGGVFKITGLGVKGIPPYSYAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBM6</accession>
    <name>A0A6I9XBM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbonic anhydrase 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911686.1">
        <property type="nucleotide sequence ID" value="XM_014056211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBM6"/>
    <dbReference type="GeneID" id="106540910"/>
    <dbReference type="KEGG" id="tsr:106540910"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911686.1"/>
        </source>
    </evidence>
    <sequence length="94" mass="10489" checksum="3095DB60E74574DC" modified="2020-10-07" version="1">MRKQSTGEALTWGYSKENGPDTWHTSYPLAKGNNQSPVAILSKDAFKDPALLPWFTGYDPGAAKNIRNTGKTLRITFDDTFDRSDSFGTLEFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC66</accession>
    <name>A0A6I9XC66_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein</fullName>
            <shortName evidence="9">Cnt-b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Centaurin-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ACAP3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">GTPase-activating protein for the ADP ribosylation factor family.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endosome membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate protein binding to PIP2 or PIP3 containing membranes.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911856.1">
        <property type="nucleotide sequence ID" value="XM_014056381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC66"/>
    <dbReference type="GeneID" id="106541054"/>
    <dbReference type="KEGG" id="tsr:106541054"/>
    <dbReference type="CTD" id="116983"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08850">
        <property type="entry name" value="ArfGap_ACAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07637">
        <property type="entry name" value="BAR_ACAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13250">
        <property type="entry name" value="PH_ACAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000007">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000025">
        <property type="entry name" value="arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000020">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000026">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042695">
        <property type="entry name" value="ACAP3_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF407">
        <property type="entry name" value="ARF-GAP WITH COILED-COIL, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="1 9" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="268"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="12">
        <location>
            <begin position="403"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="733"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="766"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="523"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="602"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911856.1"/>
        </source>
    </evidence>
    <sequence length="873" mass="98040" checksum="4396AAFD4FBE98BA" modified="2020-10-07" version="1">MTVEFEECIKDSPRFRATIDEVETDVVEIEVKLDKLVKLCSNMVDAGKAYIITNKQFVGGVRDLSQQCKKDEMISECLDKFGDSLQEMVNYHMILFDQAQRSVRQQLNNFVKEDVRKFKETKKQFDKVREDMEIALVKNAQAPRHKPHEVEEATGTLTITRKCFRHLALDYVLQINVLQAKKKFEILDAMLSFMHAQYTFFQQGYSLLHELDPYMKKLATELDQLVIDSAVEKREMEHKHALIQQRTLLQDFSYDESKVEFNVDAPNGVVMEGYLFKRASNAFKTWNRRWFSIQNSQLVYQKKLKDVLTVVVEDLRLCTVKPCEDIERRFCFEVVSPTKSCMLQADSEKLRQAWIQAVQASIASAYRESPDSCYIEKLDRTASPSTSSIDSASDSRDRSGKGETILQRVQSISGNDQCCDCGQADPRWASINLGILLCIECSGIHRSLGVHCSKVRSLTLDSWEPELLKLMCELGNSTMNQIYEAQCEEMGLKKPTAGSSRQDKEAWIKVKYVEKKFLKKLPSGEPLADNEQKPRRWSVKKCQRCNSTTKAPTTRRKYRHEAGNASPAAHSSAATLERKFRRDSLFCPDELDSLFSYFDTGAGPRGGNQASTQQPVGAGPWSETSNGPGGSGSPFLLDGGLSSDSGLGGSTDGSTDVLVFGSVVDSVTEEECEVSEDSSGEVEIEQEASDLEDLRELHPGLLVYKASRARNLPVMAEALAHGANVNWVNEEDENKTPLIQAVMGGSLIACEFLLQNGADVNQRDIRGRAPLHHATYLGHTGQVCLFLKRGANQHAVDEDGQDPLSIAVQAANADIVTLLRLARMNEEMREAEGPFGHPGDATYLDIFRDFSHMASNNPEKLNRRSLHFHPSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCI4</accession>
    <name>A0A6I9XCI4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Centrosomal protein of 89 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CEP89</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913294.1">
        <property type="nucleotide sequence ID" value="XM_014057819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCI4"/>
    <dbReference type="GeneID" id="106542136"/>
    <dbReference type="KEGG" id="tsr:106542136"/>
    <dbReference type="CTD" id="84902"/>
    <dbReference type="OrthoDB" id="6622877at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033545">
        <property type="entry name" value="CEP89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36170">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 89 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36170:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 89 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="246"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="361"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="448"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="676"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913294.1"/>
        </source>
    </evidence>
    <sequence length="782" mass="90510" checksum="DB85CE8212E86DCB" modified="2020-10-07" version="1">MPFYFRRGRRGLFKHIAHGLIPAATIAPKPAVPRTPPPRSPNPSPERPRSALAAAILVTTLTGRTIALPQPRQRSLSESDASYLQDQEDCIEPYATITELRMSPNWKNHASESCEDDMDTFASDTEKELDPDHQIIDKKESMSHEIMYATPHKIKQENLPVSSSANGEDDSSVYEPSCLIQPNVQVEEIEHPGVRFQGDHSDETPLNEKPPSSSDAVLHRKQEQMVLPREKFQKLKEQHWHLNNANQTLFSELEQTKQLMKELQLKIKKLEKENRQLKEKQNSHSEEEGTELLLLRQQAQELVDENHSLKMTVHRLNAELSRYQTKYRPISPNEGVKIGSLPMTKPIPPWLLDMKYLSPLLLAYEDRMKEKEELILEHEESMKMFKAQVEAVVKENEQLHQKLTKNTGTFISTKEWQHLQSQAKLVLEENRVLMEQLKIQGTKINDTINQHVKEVSKLTKQLVTLETEKQNQEVEMQECQKKLKELRSANKELKARVSDYITAEEHLALENKLRNQLQMEQEKREMETQELVKKLNSVQAETKSLFLEKNDLVAENKALEAELEMAQKTKRRSEKKIGLLKQHLEETMEKEMAAHQYLVNMISLAENITQERDQLLNLASCLEKENHGILNQIIEGNVHLGKLEEKVKVYKKTAAGKLGDINLKMTEQEKEFAGRTTQYQREMKHLQRLLQEKQESLDEALQQKREMESELEIIWESTTKENRRMKELLHKSLEKNPQNSVNAAYELPSTDISQKVLLGRYGFSYCDVEVSSPTHDPKEEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDJ6</accession>
    <name>A0A6I9XDJ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NEDD4 family-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NDFIP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913704.1">
        <property type="nucleotide sequence ID" value="XM_014058229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDJ6"/>
    <dbReference type="GeneID" id="106542482"/>
    <dbReference type="KEGG" id="tsr:106542482"/>
    <dbReference type="CTD" id="54602"/>
    <dbReference type="OrthoDB" id="10003116at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050699">
        <property type="term" value="F:WW domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22306">
        <property type="entry name" value="NDFIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF4">
        <property type="entry name" value="NEDD4 FAMILY-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913704.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29310" checksum="418E8F04726C8B53" modified="2020-10-07" version="1">QQQQQQQPVSRYQVLHNEDDPAESTEEQPSTSTETAPACQTEAPLPEVDTSPPPYCSIATEAAAPTEVESPNDFYPVPPPYSVATSLPTYDEAEKAKAAAMAAVAAEAAAQRHGHFRESRSLFDEIFLSPPEEEEYPPRDDFSDADQLRVGNDGIFMLAFFMAFIFNWIGFCLSFCITNTIAGRYGAICGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRFFFLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XEQ2</accession>
    <name>A0A6I9XEQ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Filamin-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FLNB</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912671.1">
        <property type="nucleotide sequence ID" value="XM_014057196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XEQ2"/>
    <dbReference type="GeneID" id="106541681"/>
    <dbReference type="KEGG" id="tsr:106541681"/>
    <dbReference type="CTD" id="2317"/>
    <dbReference type="OrthoDB" id="9612444at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000006">
        <property type="entry name" value="Filamin-B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF7">
        <property type="entry name" value="FILAMIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="68"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="191"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013912671.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="29060" checksum="CC2C3A66BC03D0C1" modified="2020-10-07" version="1">MSTQARGQAVRSDCSGGGGGSGIGEHSQGPHPRWQEHPYPKGRSDLEADSANMPATEKDLADDAPWKRIQQNTFTRWCNEHLRCVNKRIGNLQFDLSDGLRLIALLEVLSQKRMYRKYHQRPTFRQMQLENVSVALEFLEKENIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPKKPVNNAREAMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIB1</accession>
    <name>A0A6I9XIB1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Moesin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106543921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915504.1">
        <property type="nucleotide sequence ID" value="XM_014060029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XIB1"/>
    <dbReference type="GeneID" id="106543921"/>
    <dbReference type="KEGG" id="tsr:106543921"/>
    <dbReference type="OrthoDB" id="9049204at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915504.1"/>
        </source>
    </evidence>
    <sequence length="269" mass="31556" checksum="7BFD50EED15B79E7" modified="2020-10-07" version="1">MGGLLTCRQCSTRMESLKDHSFWDLTRTGVKNCFWFILVTFIVLTLLWIILYSRAPPEPGRICPKDQANLLHNISQMEAEKVTLRKQLIEMEKEMQKLRNVTNRTQGKSLQLLEKKQEEVDALSKELSGYKQSFVLTRQLLEKEKEKGQSLQSELQSAKEDLQKIQQQRDSWQKELKTLEEKLKSPDSKAEEYKRKVADLQRQLEQERKNRDFDKHERASYQNRIGELNYQIDNQKKCSTLDTFVAWALGILLISFSCCFCAGCWKLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJI2</accession>
    <name>A0A6I9XJI2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Serine/threonine-protein kinase BRSK2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106542792</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914097.1">
        <property type="nucleotide sequence ID" value="XM_014058622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJI2"/>
    <dbReference type="GeneID" id="106542792"/>
    <dbReference type="KEGG" id="tsr:106542792"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14340">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048622">
        <property type="entry name" value="BRSK1_2-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21122">
        <property type="entry name" value="KA1_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21115">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRSK1/2-like UBA" evidence="13">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="111"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="194"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="461"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013914097.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="57418" checksum="B9B3B4261BD8E807" modified="2020-10-07" version="1">MPHFIPPDCQNLLRGMIEVDASKRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSHEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQSKTMFSKSLDICEANPTFSKEDRMTSADTLGSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSIGGMPWRTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKSTGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDQPSVQQLSGSPLSNFFDVIKQLFSDEKNGQVSHPPGTPTKHSTNSKRSDSDKNDYGSRGDTKFPAGKEKAKMASSLGLQDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM69</accession>
    <name>A0A6I9XM69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein regulatory complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106543496</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DRC3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915002.1">
        <property type="nucleotide sequence ID" value="XM_014059527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM69"/>
    <dbReference type="GeneID" id="106543496"/>
    <dbReference type="KEGG" id="tsr:106543496"/>
    <dbReference type="OrthoDB" id="27917at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF12">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="351"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013915002.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="52427" checksum="E03251692EC64601" modified="2020-10-07" version="1">MSRFYDSIEPNVINEEMLQKAVEEQGPQDDAGQLAKKEGINFKEVKSLQLDFRNILKIDNLWEFVNLTKLQLDNNIIEKIEALDSLVNLVWLDLSFNNIEFIEGLDTLVKLQDLSLYNNRISQIENLDTLQELQVFSIGNNNINNLDNMIYLRKFKNLRTLNLTGNPVCDNEVYSMFIAAYLPNLVYLDFRLVEENSREMALMKYQYAIEEMRHGETLLLAKQKEWEAREKEVEYHKSAYVEYLDGAYLFDSMYAEDPEASKLMYLPGVPALLETYKTKFVEICQTLFEYGLQQHKRREDEIAIFYECLNEALKDNQDEGTRIIHEFEKKNKGTIEEIQNLGDFQMAELRLVQYNDEITKLYETLMSLEMQLVDQLEEVIKDFERNILDLVSIFIENEQGLMAQCRDLENHHHEKLLETAINTLEKIVKSEFDEEMPEDVRMLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMC8</accession>
    <name>A0A6I9XMC8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-3-phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PHGDH</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917054.1">
        <property type="nucleotide sequence ID" value="XM_014061579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMC8"/>
    <dbReference type="GeneID" id="106545116"/>
    <dbReference type="KEGG" id="tsr:106545116"/>
    <dbReference type="CTD" id="26227"/>
    <dbReference type="OrthoDB" id="1621027at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.90:FF:000005">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000038">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938:SF22">
        <property type="entry name" value="D-3-PHOSPHOGLYCERATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938">
        <property type="entry name" value="FORMATE DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="Serine metabolism enzymes domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="1">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="D-3-phosphoglycerate dehydrogenase ASB" evidence="2">
        <location>
            <begin position="133"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013917054.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="35095" checksum="5B00FC639A64185A" modified="2020-10-07" version="1">MAPAAAAGTLPLTKVLISDSLDPCCKQILEAAGIQVLEKSNLSKEQLQAEIKDVDGLIVRSATKVTAEIINAAEKLQIIGRAGTGVDNVDVEAATRKGILVMKLKHTAVLHFCSSLSQVNGQALSSAYASHTKPWIALARALGSVLHALSHQLNGSVQLITEGSALQKSSNYLIPAVAMGLLQETTQKNVNLVNGLLLAQEAGLKITTTHTDGALGNNGQLRLTATNSPHVLVGSVQGTIPCLLELNGAKFKQLVPLNGPLLFYKSKTSSVSVLPTIIDLLRKTKIEMLCYHHSSTDNSGQEWNVLSLSSALQNLQELKPHLTEVFQVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNB8</accession>
    <name>A0A6I9XNB8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD repeat-containing protein 37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13 14 15 16 17" type="primary">WDR37</name>
    </gene>
    <organism evidence="11 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912231.1">
        <property type="nucleotide sequence ID" value="XM_014056756.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912240.1">
        <property type="nucleotide sequence ID" value="XM_014056765.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912248.1">
        <property type="nucleotide sequence ID" value="XM_014056773.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912257.1">
        <property type="nucleotide sequence ID" value="XM_014056782.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912265.1">
        <property type="nucleotide sequence ID" value="XM_014056790.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912273.1">
        <property type="nucleotide sequence ID" value="XM_014056798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNB8"/>
    <dbReference type="GeneID" id="106541344"/>
    <dbReference type="KEGG" id="tsr:106541344"/>
    <dbReference type="CTD" id="22884"/>
    <dbReference type="OrthoDB" id="9984207at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000511">
        <property type="entry name" value="WD repeat domain 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000080">
        <property type="entry name" value="WD repeat-containing protein 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000152">
        <property type="entry name" value="WD repeat-containing protein 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="152"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="195"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="278"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="320"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="364"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="236"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013912231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013912240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013912257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013912265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013912273.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="54990" checksum="1A4CEC441C6B797B" modified="2020-10-07" version="1">MPTESGSCATARQAKQKRKSHSLSIRRTNSSEQERSGLQREMLEGQDSKLPSSIRNTLLELFGQIEREFENLYIENLELRREIDTLNERLAAEGQAIDGAELSKGQLKTKASHSTSQLSQKLKTTYKASTSKIVSSFKTTTSRAICQLVKEYIGHRDGIWDVSVTKTQPVVLGTASADHTALLWSIETGKCLVKYVGHAGSVNSIKFHPTEQVSLTASGDQTAHIWRYMVQLPTPQPTTDCNQMSGEDEVEFSDKDEPDGDGDGSSDCPTIRVPLTSLKSHQGVVIAADWLVGGKQAVTASWDRTANLYDVETSELVHSLTGHDQELTHCCTHPTQRLVVTSSRDTTFRLWDFRDPSIHSVNVFQGHTDTVTSAVFTVGDNVVSGSDDRTVKVWDLKNMRSPIATIRTDSAVNRINVCVGQKIIALPHDNRQVRLFDMSGVRLARLPRSNRQGHRRMVCCSAWSEDHPICNLFTCGFDRQAIGWNINIPALLQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPI8</accession>
    <name>A0A6I9XPI8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">LOW QUALITY PROTEIN: PHD finger protein 21A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PHF21A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915746.1">
        <property type="nucleotide sequence ID" value="XM_014060271.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106544113"/>
    <dbReference type="KEGG" id="tsr:106544113"/>
    <dbReference type="CTD" id="51317"/>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15523">
        <property type="entry name" value="PHD_PHF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102:SF6">
        <property type="entry name" value="PHD FINGER PROTEIN 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="482"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="320"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="30"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="556"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915746.1"/>
        </source>
    </evidence>
    <sequence length="607" mass="67501" checksum="3E2EDE1700D097E9" modified="2020-10-07" version="1">MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKRVVEQLRKNLMVKQEQPDSKFQIQPLAQTETKIQTSQPLQQQQQQQQPQQQTTAPSPNLLGSQKTVTTASMITTKTLPLVLKATATMPASVVGQRPTIAMVTVINSSQKTVLSTDAQNTPVNLQTTNKVTGPGTEAVQIVAKNTVTLVQATAPQPIKVPEFIPPPRLTPRPNFLPQVSLFRIDGNXPNCVKPPPMLAAPQLIQRPVMLTTKFTPTSLPSTQNSIHPVRVVNGQTATIAKTFPMAQLTSIVIAAPDDRLAGPQTLQISKPNVEKQTVKPQVETEEKPTEIHTVTSPAPPKPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKXRTTANPVYSGVVFEPERKKSAVTYLNSTMHPGTRKRGRPPKYNTVLGFGALTPTSPLSSHPDSPENEKTETFIFPATVQPVSLPSPSSMDGDIHEDFCSVCRKSGQLLMCDTCSRVYHLDCLDPPLKTIPKGMWICPKCQDQMLKKEEAIPXPGTXAIVHSYIAYKTAKEEEKQKLLKWSSDLKQEREQLEQKVKQLSNSITVSINHCQVIIYLYFLAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XR52</accession>
    <name>A0A6I9XR52_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Roundabout homolog 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106537515</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013907185.1">
        <property type="nucleotide sequence ID" value="XM_014051710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XR52"/>
    <dbReference type="GeneID" id="106537515"/>
    <dbReference type="KEGG" id="tsr:106537515"/>
    <dbReference type="OrthoDB" id="428111at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000055">
        <property type="entry name" value="roundabout homolog 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000065">
        <property type="entry name" value="roundabout homolog 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000008">
        <property type="entry name" value="roundabout homolog 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000058">
        <property type="entry name" value="roundabout homolog 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF236">
        <property type="entry name" value="ROUNDABOUT HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="42"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="150"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="264"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="361"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="672"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="726"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013907185.1"/>
        </source>
    </evidence>
    <sequence length="745" mass="81454" checksum="DC5F5A9B80F4F211" modified="2020-10-07" version="1">MTIMDVQTSDSGYYMCQAISVAGSILAKALLEVEDVTTDRIPPIIRRGPANQTLAIDSTALLQCHVTGNPLPSIQWLKDGQRIVGSDPRMSLLDNGTLQIINLQISDSGLYVCIATSSTGETRWSGFLAVQENGVDLQMQLPEPNLLPGPPSAPLVIDVTKNSVALIWKPNPGNGGAPITSYIIEAFSQSSGSTWQTVADDVKLEKHTVSGLNPNTIYLFLVRAVNSYGLSDPSPVSEPVRTQDVSPTRQGVDHRQVQRELGDVVVQLQEPVVLTSTTIQVTWTVDRQAQFIQGYRVMYRPKNSAWLVQDVKSPVERNTVLVDLRKGQEYEVKIRPYFDEFQGMDSEVLLVRTPEEAPSAPPQGVSVMTVGTSNSTSISISWEPPPPGEQNGIIQDYRVWCLGNETRYHINKTVDGTMRSTVLMGLIPGMIYRTEVAATTSAGIGVRSAPITIQIKPLPDQDTVPVDNGDSVSLAEQITGVVKQPAFIAGIGGACWVILMCFSVWIYCRRKKRKEMSHYTASFAYTPAVSFPHTDGPARVGSSSRPGMLAMATSASSYPWLADSWPAPNLLRNGKTKDPGMACCTGNHNVTERYYNDAGISNYIAQTEKFGTGVSDGPIYSTIDPASDDMRTFNGAFSQHTTPYATTPVVPYGATHLHSPDEEEGQWNNEPGISTIQYAQPDRGRVESVAKPGKQKSMGKPVKTPSLNWTEVLPPPPSANELSQYVEEEEEEEEEEEEEEELELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRI4</accession>
    <name>A0A6I9XRI4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Syntaxin-11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">STX11</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the SNARE proteins SNAP-23 and VAMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913292.1">
        <property type="nucleotide sequence ID" value="XM_014057817.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913293.1">
        <property type="nucleotide sequence ID" value="XM_014057818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRI4"/>
    <dbReference type="GeneID" id="106542167"/>
    <dbReference type="KEGG" id="tsr:106542167"/>
    <dbReference type="CTD" id="8676"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048787">
        <property type="term" value="C:presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000015">
        <property type="entry name" value="Syntaxin 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000022">
        <property type="entry name" value="Syntaxin 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF30">
        <property type="entry name" value="SYNTAXIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="204"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013913293.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="34144" checksum="C228FFE45C3477CF" modified="2020-10-07" version="1">MRDRLLEFYELSRLYSQHTGDAEDDFQRPHETLFFETDHAVANLYKNIQEIRTNNHHLKEDVRRLRKQNSRFLTSIRRFSSIKRDTNTIAKDIKARGEEIHRKLQVLRDLSEDAETKYGENAIVTRVCKDHYVDLMHAFQEAMFNYNETEMKERDNCKVRIQRQLEIMGKEVSGNQIEDIIEQGRWDVFSENLLSDTKGARSALNEIETRHKELMKLESRIKEVHELFLQVALLVEEQADTFDVIAFNMQNVQEYVGEAKDEVRRALEYRRKHPCRTILCCCLSCLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSS6</accession>
    <name>A0A6I9XSS6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Glutamine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">QARS</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Glutamine--tRNA ligase. Plays a critical role in brain development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with RARS1. Part of a complex composed of RARS1, QARS1 and AIMP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013919119.1">
        <property type="nucleotide sequence ID" value="XM_014063644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSS6"/>
    <dbReference type="GeneID" id="106546702"/>
    <dbReference type="KEGG" id="tsr:106546702"/>
    <dbReference type="CTD" id="5859"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1290:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2420:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000008">
        <property type="entry name" value="probable glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000049">
        <property type="entry name" value="Probable glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000006">
        <property type="entry name" value="Putative glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007638">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007639">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042558">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042559">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04558">
        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04557">
        <property type="entry name" value="tRNA_synt_1c_R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 17 25" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="22">
        <location>
            <begin position="16"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="21">
        <location>
            <begin position="174"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="19">
        <location>
            <begin position="271"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="20">
        <location>
            <begin position="573"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="23">
        <location>
            <begin position="686"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013919119.1"/>
        </source>
    </evidence>
    <sequence length="784" mass="88683" checksum="F702D2535240FBD2" modified="2020-10-07" version="1">MAGAGGSMAADESDPLRLFVSIGLSESKARETLRNEALTALLREAVVQAQRILGPVIDKTVGTLLYNVASRLKDSKRLGDLVEYIATKKIITDLQLNAALEYLKNHPVDPINTADFDRECGIGVVVTPEQIEEAVEAAICKHRARLLSERYHFNMGVLMGEARSKLKWADGKIIKNEVDLQVLNLLGPKTEADLEKKSKTPKARNTEPEQGKSMVVENGEPHQETKSLLEQLRGEALKFHKPGENYKTEGYIVTPNTMNLLKKHLEITGGQVRTRFPPEPNGILHIGHAKAINFNFGFAKANGGICFLRYDDTNPEKEEEKYFTGILDIVEWLGYTPYAVTHASDYFDELYALAVDLVRRGHAYVCHQKVEEIKGHNPPPSPWRDRPIEESLLLFEDMRKGKFGEGEATLRMKMVMEDGKMDPVAYRIKYTPHHRSGDKWCIYPTYDYTHCLCDSLENITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLVYTVVSKRKIIRLVEAGAVRDWDDPRLFTLTALHRRGFPPEAINNFCAKVGVTVAQCTTEPHMLEACVREVLNEQAPRVMAVLEPLRVTITNFPAPHKNIIIRVPNFPADETKGFHEVPFQPTLYIEQSDFREVPDKGYKRLALGQPVGLRHTGYVIAVQNIIKDTNGQVKEVEVTCTKSEAAEKPKAFIHWVSDPLVCEVRQYEQLFLHKNPEDSTEVPGGFLSDINPDSLHVIEEALVDRSVYGAKPFTKFQFERLGYFSLDPDSTNAKLVFNRTVTLKEDPGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT85</accession>
    <name>A0A6I9XT85_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">5'-nucleotidase</fullName>
            <ecNumber evidence="3 9">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NT5C3A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the pyrimidine 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_013907930.1">
        <property type="nucleotide sequence ID" value="XM_014052455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT85"/>
    <dbReference type="GeneID" id="106538068"/>
    <dbReference type="KEGG" id="tsr:106538068"/>
    <dbReference type="CTD" id="51251"/>
    <dbReference type="OrthoDB" id="10014216at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07504">
        <property type="entry name" value="HAD_5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.340:FF:000001">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000032">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.340">
        <property type="entry name" value="Pyrimidine 5'-nucleotidase (UMPH-1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006434">
        <property type="entry name" value="Pyrimidine_nucleotidase_eu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01544">
        <property type="entry name" value="HAD-SF-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045:SF14">
        <property type="entry name" value="CYTOSOLIC 5'-NUCLEOTIDASE 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05822">
        <property type="entry name" value="UMPH-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01128">
        <property type="entry name" value="C1.4:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026775675" description="5'-nucleotidase" evidence="10">
        <location>
            <begin position="19"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013907930.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="36536" checksum="AEDEE5C990D64976" modified="2020-10-07" version="1">MQALHFWSLLVTCAVAVGKDQVLKKQGCLKISAQMPEFQKKTVHIKDPEKVEEIICGLIKGGAAKLQIITDFDMTLSRFTHNGKRCPTCHNIIDNCNLITEECRTKLFHLKEIYYAIEIDPNLTVEEKYPYMVEWYNKSHALLIEQGLQKNKLAEIVRESDVMLKEGYEFFFDKLQEHNIPVFIFSAGIGDILEQIIHQEAVYRSNIKVVSNFMEFDDNGILKGFKGELIHVYNKHDGALKNTEYFKQLKDNSNIILLGDSQGDLTMADGVANVEHILKVGYLNDKVEQLLEKYMESYDIVLVKDDSLDVANSILQKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUB8</accession>
    <name>A0A6I9XUB8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein FAM183A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FAM183A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP144 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919699.1">
        <property type="nucleotide sequence ID" value="XM_014064224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUB8"/>
    <dbReference type="GeneID" id="106547158"/>
    <dbReference type="KEGG" id="tsr:106547158"/>
    <dbReference type="CTD" id="440585"/>
    <dbReference type="OrthoDB" id="446290at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029214">
        <property type="entry name" value="CFAP144"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865:SF3">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14886">
        <property type="entry name" value="FAM183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013919699.1"/>
        </source>
    </evidence>
    <sequence length="136" mass="15747" checksum="C65920CD7847C76A" modified="2020-10-07" version="1">MAAFKGGVKEPLDPVSQNKIFTETVRKELRCQRLHTEFALNPLLKVYALTGKPASWHDNLEEPEDAKFLKLIHHAALEPPKKYTEPQTESQEIGWFSTPLVSINRDDSRLHFPSRSTEISRYMAALWRLRQMTKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUE9</accession>
    <name>A0A6I9XUE9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sorbin and SH3 domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106547180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919724.1">
        <property type="nucleotide sequence ID" value="XM_014064249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUE9"/>
    <dbReference type="GeneID" id="106547180"/>
    <dbReference type="KEGG" id="tsr:106547180"/>
    <dbReference type="OrthoDB" id="73680at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031589">
        <property type="term" value="P:cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003127">
        <property type="entry name" value="SoHo_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF64">
        <property type="entry name" value="SORBIN AND SH3 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02208">
        <property type="entry name" value="Sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00459">
        <property type="entry name" value="Sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50831">
        <property type="entry name" value="SOHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SoHo" evidence="7">
        <location>
            <begin position="326"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="582"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="656"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="394"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013919724.1"/>
        </source>
    </evidence>
    <sequence length="818" mass="92077" checksum="BA613388D0F0BE81" modified="2020-10-07" version="1">TSETEKADVVNGLALNINQHEKDQESGKICTGKGAVTLWASPASEKSPRVSHPKSTQDSQHPKIHSAEQQGETNTDGWRRLSGVDSNREILSVKDYWSVRPNFAESKPRAATSSPTTSVQTEIITGSFQQIQPGREEARPSTSPAPLRHFDQGLEISNDVSPGFLLALPTLDDFIPPHLQKIPSHQYPLFSPQPALHTQAKLPSLSSPPPQLIPPATETSSSILETESTGMVFQTDTCPQPHEASKPSSGPAVSMTINRSLGVYPSTTIVNPTIVLLQHNREQQKRLSSLADSVPDPPVVEKVVGVSLRPKLNPSKEDAVEEQKRIVNNSQPMDDDSLDSIGIPLRNSERSKDWYKIMFKQIHRLTKDPPEENPYCPTYIFPELPAFQPKSKEQNPYSPTYQFPVSTPSPRSEEMSPRDISPEEIDLKNEPWYKFFSELEFGKPPPKKIWDYTPGDCSILTREDRKVDPGKNHCLYHTELDADIGKMEELYKAPDQKQLKNAASSPPPESLSEQLSPYCPFYAILWQKLLEDQRRLKREQEEADIAARRHTGVIPTHHQFITNERFGDLLIRDDTIKRKSGAEMRAARVKFDFKAQTLKELPLQKGDIVYIYKPIDQNWYEGEHHGRVGIFPQSYIELLNPTEKIQTKKTSSLQILEYGNAIAKFSFSGDTAVEMSFRKGERITLIRQVDENWYEGKILGTSRQGIFPGSYVDILKHPVLKDSSGYAYQPPNCSMAFPKPSLRPGPELTESEKNYVEVVCNEILNIAEKSVHYCSTLSQPVFCHKGPFSDNKSSFIISQQPQVLPLETGLDRNHATRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVG9</accession>
    <name>A0A6I9XVG9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Fibronectin type III domain-containing protein 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">FNDC4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918092.1">
        <property type="nucleotide sequence ID" value="XM_014062617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVG9"/>
    <dbReference type="GeneID" id="106545903"/>
    <dbReference type="KEGG" id="tsr:106545903"/>
    <dbReference type="CTD" id="64838"/>
    <dbReference type="OrthoDB" id="5843172at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000117">
        <property type="entry name" value="Fibronectin type III domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052120">
        <property type="entry name" value="FNDC_type_III_4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026871633" evidence="10">
        <location>
            <begin position="27"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="29"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="179"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013918092.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="24295" checksum="4CB348B97A3126AD" modified="2020-10-07" version="1">MAPIPTYLNPAAVLVLVSCDLCLVRANRPPSPVNVTVTQLKANSATVSWDVPEGDVIIGYAISQQRQDGQMQRFIREVNTTNRACVLWDLAEDADYIIQVQSIGLHGESQASKRVHFRTLKQSDRLPSNSSNQGDITMEGLDKERQLQTGEIIIIVVVLLMWAAVIALFCKQYDIIKDNDSNNNKEKTKPSSEHSTPERPTGGLLRSKKKSPSINIIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVW5</accession>
    <name>A0A6I9XVW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Importin-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IPO9</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914783.1">
        <property type="nucleotide sequence ID" value="XM_014059308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVW5"/>
    <dbReference type="GeneID" id="106543323"/>
    <dbReference type="KEGG" id="tsr:106543323"/>
    <dbReference type="CTD" id="55705"/>
    <dbReference type="OrthoDB" id="431626at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056840">
        <property type="entry name" value="HEAT_IPO9_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25018">
        <property type="entry name" value="HEAT_IPO9_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="39"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="932"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="938"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013914783.1"/>
        </source>
    </evidence>
    <sequence length="1036" mass="115670" checksum="9B4F1D3B64751EA2" modified="2020-10-07" version="1">MSGAVAGGGPGPVAQGLKEALVETLNGILCPVQGVRAAAEEQVKVLEVTEEFGVHLAELTVDPQGALAIRQLASVILKQYVETHWCSQSEKFRPPETTERAKAAIRELLPNGLRESISKVRSSVAYAVSAIAHWDWPEAWPELFNLLMEMLVSGDLNAVHGAMRVLTEFTREVTDTQMPLVAPVILPEMYKIFTMAEVYGIRTRSRAVEIFTTCAQMICNMEELEKGAAKILIFPVVQQFTEAFVQALQMPDGPTSDSGLKMEVLKAVTALVKNYPKHMISSMQQILPIVWNTLTESAAFYVRTEVNDTEEVEDPVDSDGEVLGFENLVFSIFEFVHALLENHKFKSTVKKALPELIYYIILYMQITEEQIKVWTANPQQFVEDEDDDTFSYTVRIAAQDLLLAVAADFQNESATALAAAATRHLQEAEQAKNSGAEHWWKIHEACMLALGSVKSLITDCVKNGRIHFDMHGFLTNVLLADLNLSVSPFLLGRALWVASRFTVAMSPELIQQFLQATVSGLHETQPPSVRISAVRAIWGYCDQLKISDSTHVLQPFLPSVLDGLIHLAAQFSSEVLNLVMETLCIVCTVDPAFTANVENKICPFTIAIFLKYSNDPVVASLAQDIFKELAQIEACQDAMQMRLIPTLVSIMQAPVDKIPAGLCATSIDILTTVVRNTKPPLSDLLIFQAFPAVAQCSLHTDDNATMQNGGECLRAYISVALEQIAQWHDEQGHNGLWYVMQVVSQLLDPRTSEFTAAFVGRLVSTLISKVGSELGENLDQILRAILSKMQQAETLTVMQSLIMVFAHLVHSQLEPLLEFLCSLPGPTGKPALEFVMSEWMSRQHLFYGQYEGKVSSVALCKLLQYGINTDDKRLQDIRVKGEEIYNMEEGIRTRSKAAKNPERWTNIPLLVKILKLIINELSNAIEANASRQTAAEWSQEESNDMWEDQEDEEEEENEGLTGQLLSDILATNKYDEDYYEDDDEDDPDALKDPLYQIDLQAYLTEFLCQFAQHPCYTMFSDHLNENERRVLQTIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXJ1</accession>
    <name>A0A6I9XXJ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribosomal protein S6 kinase</fullName>
            <ecNumber evidence="14">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">RPS6KA5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013918897.1">
        <property type="nucleotide sequence ID" value="XM_014063422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXJ1"/>
    <dbReference type="GeneID" id="106546533"/>
    <dbReference type="KEGG" id="tsr:106546533"/>
    <dbReference type="CTD" id="9252"/>
    <dbReference type="OrthoDB" id="6764942at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000648">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000109">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000157">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000208">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000686">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016239">
        <property type="entry name" value="Ribosomal_S6_kinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000606">
        <property type="entry name" value="Ribsml_S6_kin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="40"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
        <location>
            <begin position="310"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="417"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="730"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="738"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="773"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="535"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="46"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 17">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="423"/>
            <end position="431"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 17">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000606-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000606-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013918897.1"/>
        </source>
    </evidence>
    <sequence length="788" mass="89259" checksum="2D43E799A2DB08AF" modified="2020-10-07" version="1">MERPAGGAEEEGEPSLLTVRHELKNANLTGHTEKVGIENFELLKILGTGAYGKVFLVRKISGHDAGKLYAMKVLKKATIIQKAKTTEHTKTERQVLEHIRQSPFLVTLHYAFQTDTKLHLILDYINGGELFTHLSQRERFNEDEVRIYIGEIVLALEHLHKLGIIYRDIKLENILLDSDGHVVLTDFGLSKEFVNDENERAYSFCGTIEYMAPDIVRGGNTGHDKAVDWWSLGVLMYELLTGASPFTVDGERNSQTDISRRILKSEPPYPQEMTALAKDLIQCLLMKDPKKRLGCGSGSADEIKQHSFFKKINWNDLAAKKVSAPFKPIIRDELDVSNFAEEFTEMDPTYSPATIPEAADKLFQGYSFVAPSILFKHNAAIMDPVYFLTKHNQPGVTNIARSAMMKDSPFYLHYELDLKEKPLGEGSFSICRKCLHKKTRHEYAVKIISKRLEVNTQREIAALKLCEGHPNVVKLHEIYHDQLHTFLVMELLKGGELLERIQKKKYFSETEASYIMRRLVSAVSHMHDVGVVHRDLKPENLLFTDENDNSEIKIIDFGFARLKPSDNQPLRTPCFTLHYAAPELLNHNGYDESCDLWSLGVILYTMLSGQIPFQSQDTSIICTNVLETMKKIKRGEFSFEGDAWKNVSKEAKDLIQGLLTVDPNKRIKMTSLRHNDWLQDGCQLSSNPLMTPDNLGSSGAAIHVKATFNAFNKYKREGFCLQNVDKAPLAKRRKMKKTSTSTETRSSSSESSHSSSSQSYDKTTPTKTLQTTHPIDSNNSDNIFQFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY00</accession>
    <name>A0A6I9XY00_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">TP53-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TP53BP1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013915478.1">
        <property type="nucleotide sequence ID" value="XM_014060003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY00"/>
    <dbReference type="GeneID" id="106543897"/>
    <dbReference type="KEGG" id="tsr:106543897"/>
    <dbReference type="CTD" id="7158"/>
    <dbReference type="OrthoDB" id="129353at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140005">
        <property type="term" value="F:histone H4K20me2 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045830">
        <property type="term" value="P:positive regulation of isotype switching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17745">
        <property type="entry name" value="BRCT_p53bp1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17724">
        <property type="entry name" value="BRCT_p53bp1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20383">
        <property type="entry name" value="Tudor_53BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000021">
        <property type="entry name" value="Tumor suppressor p53-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000019">
        <property type="entry name" value="Tumor suppressor p53-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000003">
        <property type="entry name" value="Tumor suppressor p53-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000005">
        <property type="entry name" value="Tumor suppressor p53-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015125">
        <property type="entry name" value="53-BP1_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047249">
        <property type="entry name" value="BRCT_p53bp1-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047250">
        <property type="entry name" value="BRCT_p53bp1-like_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047252">
        <property type="entry name" value="TP53BP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321:SF3">
        <property type="entry name" value="TP53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321">
        <property type="entry name" value="TUMOR SUPPRESSOR P53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09038">
        <property type="entry name" value="53-BP1_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18428">
        <property type="entry name" value="BRCT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BRCT" evidence="20">
        <location>
            <begin position="495"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="20">
        <location>
            <begin position="630"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="102"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="410"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00073180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013915478.1"/>
        </source>
    </evidence>
    <sequence length="737" mass="81353" checksum="417BA9DB7F650439" modified="2020-10-07" version="1">MKGCLEIFWTFFFLQKEEEFELPHPPAGKVLQRHVRTIREVRTLITRVITDVYYKDGTEVERKVVEEREEPHVDCYECEVDVSPSRTGAFSLTSGDLGDLSSFSSKASGPQHTSSGGSSALSATRSRVNSVGPRRGSEDLALPAARKPSPKKGTGFCPAHRDHGKAAAGDETEDPGPSNQQCRGTPLTPRGRGRRGRPTSRSTGVRGSMQVEAELSGGASLDGQPSLGPHLERRERAEPPRCRSGSPEIPLQEQLGASDDPRLPPSGSCLVGLRVVAKWSSNGYFYSGTITQDVGGAKYRLLFDDGYECDVLARDVLLCDPLPLETEVTALSEDEYFSAGVVKGHRKEAGELFYCIEKDGQQKWYRRTAVILSLEQGNRLREQFGLGPCQPATPLTRAADISLDNLVEGKRKRRSIASPSPSSGSTTPVRKSPEPLSSKRKLAAVSEEEQSPAKRGRRLALAGAANAREMANPSSSGKYSGDHLALDHRWGPLPQNKTLFLGYAFLLSMANTTEKLSSHQKTVVSSEEEEFVERVPYNKPYTELQLQAGGGFILEDFNESQCSAAYECLLIADQHCRTRKYFLCLARGIPCVSHMWVHDSCLANQTQNYKNYLLPAGYSLEEERLLEWHPRTNPFQKLRVLLVSDESQNFLELWSEILMMGAAASVKQQESSTWKNDVSLGVFDVVVTDASCPAAMVPCAKALQLPVVTQEWVIQSLIAGERADFKYLKYQHDYVPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XY54</accession>
    <name>A0A6I9XY54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HERPUD2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Could be involved in the unfolded protein response (UPR) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919122.1">
        <property type="nucleotide sequence ID" value="XM_014063647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XY54"/>
    <dbReference type="GeneID" id="106546707"/>
    <dbReference type="KEGG" id="tsr:106546707"/>
    <dbReference type="CTD" id="64224"/>
    <dbReference type="OrthoDB" id="21589at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17119">
        <property type="entry name" value="Ubl_HERP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000046">
        <property type="entry name" value="Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039751">
        <property type="entry name" value="HERPUD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943:SF5">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UBIQUITIN-LIKE DOMAIN MEMBER 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UNIQUITIN-LIKE DOMAIN HERPUD PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="10"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919122.1"/>
        </source>
    </evidence>
    <sequence length="400" mass="44874" checksum="39EF96B78EF4ECAF" modified="2020-10-07" version="1">METPEAGAPVTLIIKAPNQKYMDQTISCFLDWTVGRLKCHLSKVYPSKPSTKDQRLVYSGRLLPDHLQLKDILRKQDEYHMVHLVCSSRTPPNSPKPDRESHESSASSNSLSSEHLGSISPSPSQETVYRTVSNSEDVRHRNLAQTQNNQMQSHPFSYLLQGVDNQFPVQGISTGCPVYPTISPLQMLWWQQMYAHQYYMQYQAALSAQENLSPESTSPPVPEPSNSDYVPANEAPAAPNVAPQENRPINQNVQMNAQGGPVINEEDFNRDWLDWIYTFSRAAILLSIVYFYSSFSRFVMVMGAMLLVYLHQAGWFPFRQEGIQQPAANNADINHDGQNVNNAGLEEMERLMDEGIEEDSGEDIGDVNAEQPPGFMASAWSFITTFFTSLIPEGPPQVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYJ8</accession>
    <name>A0A6I9XYJ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein Daple-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106546523</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCDC88 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918886.1">
        <property type="nucleotide sequence ID" value="XM_014063411.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106546523"/>
    <dbReference type="KEGG" id="tsr:106546523"/>
    <dbReference type="OrthoDB" id="10254988at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000035">
        <property type="entry name" value="girdin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF31">
        <property type="entry name" value="PROTEIN DAPLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="11"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1400"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="249"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="457"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="971"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1049"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1126"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1203"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1343"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1400"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1412"/>
            <end position="1447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013918886.1"/>
        </source>
    </evidence>
    <sequence length="1476" mass="173367" checksum="1C18D0913D410F38" modified="2020-10-07" version="1">MDVTVSELLELFLQSPLVTWVKTFGLLGNDNEDKLALYMNLADGVFLNKIMLQIDPRPTNQRFNEHVNNDVNLRIQNLTILVRNIKTYYQEVLQQLIIMNLPNVLTIGKDPLSGKSMDEIKKLLLLVLGCAVQCERKEEFIERIKQLDIETQAAIVSHIQEVTHNQENVFDLQWLELPAMAPEELESLSRNMVFHLKRLIDERDECTEVIVDLTQDRDYLQSQTPLSPLKTSSPASSPNLASRLSNEDKQHLSVELADAKAKLRRIRQELEEKSEQLVDTRHEVDQLVLELQKIKQENITLMADARSARAYRDELDSLREKANRVERLEMELVRCKEKLHDVDFYKARMEELREDNIVLIETKTMLEEQLMISRARGDKLHEIEKENLQLKSKLHDIELDRDTDKKRIEELLEENMVLEIAQKQSMNESVHLGKELEQLSKNTDLSDTRKSFVFELNECASSRILKLEKDNQSLQNIIQELRDASVVTEENSLKFVELQNENQQLGQKIEKLQNQIEKEKQSNQDLEALSEELLKEKQDLKNTMEALKADQERQIQDLEKETDHLNQVVFSLRQRSQVSTEAKVKNIEMENKMLHEAVSESNSKLSKLEFEKKQLQKDFEQVKEKVERVEEMDKELHRLEKVNDQLAKKVAVMKITTEKVEVLEQENKDLEVENRKLKKSLDTLHNVSIQLENLEKNNKQLDDKNLELRQIVETMRFTSTKLAQIEAENKELEKEKEELQKNIVMLKVLLKKSERLELSYQSMNAENQKLQQILESNNKNVQLLEKELQRVEDDNQSLQRKLEELKISSKQVEWLEKEKKLLEQEVFQLDKDKKMLEKETKRLWQQVELKDANLDENTIKLTAIEKEYKILERELARVSASSNKMKEFEKNNKDLQKQVTIDKRTLDTLREDLVLEKLKSQKLSSEMDKLDMELEKMGLNKELLLQDDNGNSDKKYKILESQTECILKTTLASKEEKIVALEAKLQEFVNLNQQLQNELNMMRKDFECLKQKQEEGFFVQNQPLENCQKEATMELLKVKDRAIDLERNNAALQTGIQLHKEQLARLEVQNSSLNSQILTLQKQNAFLQEHNTAMQTQTAKLQVENSTLNSQSTSLMTQNTLLQNQQLAKEHENDNLLKQKEQLKAEYESLLQDHEHLVLLHERQSTEYEALIKQHSNLKSLHKNLDLEHKDLGERYSSLIEHKTELEEMEMILKTEKDVLQQEKRTSTIMIAENQKLREEIERMNMSHIKLKAEYEGLHSHTKELKTSLNNSQLELNRWQARYDELKEQHQNMDITLTKLDNHCELLGRLKGNLEEENHHLLSQIQLLSHQNQMLLEQNMENKEQYHEEQKQYIDKLNDLRRHKEKLEEKIMDQYRFYDPTPKKKNHWIGAKALVKFMKAKKEPTRERMKSSADSSPSQLESMETAESQTPSQLPKQQKSLETTSKDFSFVEDKEHKAGPMVKGRHFISLESELAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ97</accession>
    <name>A0A6I9XZ97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger and BTB domain-containing protein 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Zinc finger protein 288</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22 23 24 25 26" type="primary">ZBTB20</name>
    </gene>
    <organism evidence="21 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22 23 24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">May be a transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses. Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Can homodimerize. Binds to DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919653.1">
        <property type="nucleotide sequence ID" value="XM_014064178.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919661.1">
        <property type="nucleotide sequence ID" value="XM_014064186.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919668.1">
        <property type="nucleotide sequence ID" value="XM_014064193.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919676.1">
        <property type="nucleotide sequence ID" value="XM_014064201.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919682.1">
        <property type="nucleotide sequence ID" value="XM_014064207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ97"/>
    <dbReference type="GeneID" id="106547120"/>
    <dbReference type="KEGG" id="tsr:106547120"/>
    <dbReference type="CTD" id="26137"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18208">
        <property type="entry name" value="BTB_POZ_ZBTB20_DPZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000039">
        <property type="entry name" value="Zinc finger and BTB domain containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000304">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000315">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000426">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF22">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="19">
        <location>
            <begin position="120"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="617"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="725"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="367"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="417"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="420"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013919653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013919661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013919668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013919676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013919682.1"/>
        </source>
    </evidence>
    <sequence length="751" mass="82915" checksum="06B506C0795D0A12" modified="2020-10-07" version="1">MSALSPKLGVIYLKSDIQKPLKKPKTTEIPKSEENEITQTGAFVAKSGLPCLNFEAISSLSPDLIHSSHSLRNLHVYTGSPDCNNSCKGMTERIHSINLHNFSNSVLETLNEQRNRGHFCDVTVRIHGSMLRAHRCVLAAGSPFFQDKLLLGYSDIEIPSVVSVQSVQKLIDFMYSGVLRVSQSEALQILTAASILQIKTVIDECTRIVSQNVGDIYPLIQDSAQETPRGTPESATSGQSSDTESGYLQSHSQHSVDRIYSALYACSMQNGSGERSFYSGAVVSHHETALGLSRDHHMEDPSWITRIHERSQQVERYLSTTPETTHCRKQPRPVRIQTLVGNIHIKQEMEDDYDYYGQQRVQILEHNESGECTEDTDQAEGAESEPKGESFDSGVSSSIGTEPDSVEQQFMTGLGREVQQEPTQPEQNDVSAESIQQQHTDTNSSSPERGNDVERDSALITVCNSTEKGTLQPITTTVSQSLPSTQLYLRQTETLTSNLRMPLTLTSNTQVIGTAGNTYLPALFTTQSAGSGPKPFLFSLPQPLGQQAQFVTVSQPGISTFTAQLPAPQPLAPSSGHSTAGGQGEKKPYECTLCNKTFTAKQNYVKHMFVHTGEKPHQCSICWRSFSLKDYLIKHMVTHTGVRAYQCSICNKRFTQKSSLNVHMRLHRGEKSYECYICKKKFSHKTLLERHVALHSATNGTSNTTSTGARALPAGVMACTEGTTYVCSVCPAKFDQIEHFNDHMRLHVSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZY0</accession>
    <name>A0A6I9XZY0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pseudouridine-5'-phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Haloacid dehalogenase-like hydrolase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Haloacid dehalogenase-like hydrolase domain-containing protein 1A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Pseudouridine-5'-monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PUDP</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Dephosphorylates pseudouridine 5'-phosphate, a potential intermediate in rRNA degradation. Pseudouridine is then excreted intact in urine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>psi-UMP + H2O = pseudouridine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10944"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17802"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58380"/>
            <dbReference type="EC" id="3.1.3.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.96" evidence="8"/>
    <dbReference type="RefSeq" id="XP_013919541.1">
        <property type="nucleotide sequence ID" value="XM_014064066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZY0"/>
    <dbReference type="GeneID" id="106547040"/>
    <dbReference type="KEGG" id="tsr:106547040"/>
    <dbReference type="CTD" id="8226"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990738">
        <property type="term" value="F:pseudouridine 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07529">
        <property type="entry name" value="HAD_AtGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000055">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045228">
        <property type="entry name" value="Gpp1/Gpp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013919541.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="26208" checksum="8E31F8C77A25A7A9" modified="2020-10-07" version="1">MATSLCHPPSAQPVTHLIFDMDGLLLDTEHLYTIVFQNICNRFGKTYTWDVKSLAMGKKALEGAEIIRDALDLPITKEELLHESKIMQEKLFPIATLMPGVEKLIHHLHKHDIPIAVATSSSRVTFEMKTSRHKDFFALFHHIVLGDDPEVKHGKPQPDVFLVCAERFDPPPCPEKCLVFEDAPNGVKASLKAGMQVIMIPDENLNKDLTKEASLVLGSMSDFKPELFGLPPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y201</accession>
    <name>A0A6I9Y201_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RPL27</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910815.1">
        <property type="nucleotide sequence ID" value="XM_014055340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y201"/>
    <dbReference type="GeneID" id="106540275"/>
    <dbReference type="KEGG" id="tsr:106540275"/>
    <dbReference type="CTD" id="6155"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000001">
        <property type="entry name" value="60S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018262">
        <property type="entry name" value="Ribosomal_eL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01107">
        <property type="entry name" value="RIBOSOMAL_L27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="6">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013910815.1"/>
        </source>
    </evidence>
    <sequence length="136" mass="15828" checksum="6DF2DC4819E37A32" modified="2020-10-07" version="1">MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTASMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTIVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2J8</accession>
    <name>A0A6I9Y2J8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C14orf132 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106540465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911045.1">
        <property type="nucleotide sequence ID" value="XM_014055570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2J8"/>
    <dbReference type="GeneID" id="106540465"/>
    <dbReference type="KEGG" id="tsr:106540465"/>
    <dbReference type="OrthoDB" id="9943350at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013911045.1"/>
        </source>
    </evidence>
    <sequence length="83" mass="8979" checksum="9E7B60950C006858" modified="2020-10-07" version="1">MDLSFMAAQLPVMGGVFMDSSNEEFSTEYSLFNSSANDYTTASVPNQPEETARSSNDAILLWIAIIATIGNIVVVGVVYAFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2R3</accession>
    <name>A0A6I9Y2R3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106545397</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013917418.1">
        <property type="nucleotide sequence ID" value="XM_014061943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2R3"/>
    <dbReference type="GeneID" id="106545397"/>
    <dbReference type="KEGG" id="tsr:106545397"/>
    <dbReference type="OrthoDB" id="414243at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03208">
        <property type="entry name" value="GST_C_Alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000005">
        <property type="entry name" value="Glutathione S-transferase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003080">
        <property type="entry name" value="GST_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF107">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01266">
        <property type="entry name" value="GSTRNSFRASEA"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01205">
        <property type="entry name" value="AMPS.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00363">
        <property type="entry name" value="AMPS_(cytGST):_Alpha-__Mu-__Pi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="85"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013917418.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="25596" checksum="280C25D878757343" modified="2020-10-07" version="1">MAGKPKLHYTRGRGKMESIRWLLAAAGVEFEEQFLETNKDLEKLRSDGCLLFQQVPMVEIDGMKLVQTRAILCYIAAKYNLYGKDLKERALIDMYVEGTTDLMGMIMMLPLQPAENKEKQKDLIIERAATRYFPVYEKVLKDHGQNFLVGNQFSWADVHLLEAILMVEEFKSDALAKFPLLQAFKTRISNIPTIKKFLQPGSQRKPPMDDNMLAQIKKIFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4S5</accession>
    <name>A0A6I9Y4S5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC106546761</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919188.1">
        <property type="nucleotide sequence ID" value="XM_014063713.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013919189.1">
        <property type="nucleotide sequence ID" value="XM_014063714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4S5"/>
    <dbReference type="SMR" id="A0A6I9Y4S5"/>
    <dbReference type="GeneID" id="106546761"/>
    <dbReference type="KEGG" id="tsr:106546761"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000004">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="14">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013919188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013919189.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="13507" checksum="A3FE4ED7A0AEA4AA" modified="2020-10-07" version="1">MSGRGKSGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLSAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTQSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4S8</accession>
    <name>A0A6I9Y4S8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Leucine-rich repeat neuronal protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LRRN3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919198.1">
        <property type="nucleotide sequence ID" value="XM_014063723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4S8"/>
    <dbReference type="GeneID" id="106546766"/>
    <dbReference type="KEGG" id="tsr:106546766"/>
    <dbReference type="CTD" id="54674"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000481">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000056">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000074">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000090">
        <property type="entry name" value="Leucine-rich repeat neuronal protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF213">
        <property type="entry name" value="INSULIN LIKE GROWTH FACTOR BINDING PROTEIN ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027042966" evidence="6">
        <location>
            <begin position="23"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="421"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013919198.1"/>
        </source>
    </evidence>
    <sequence length="711" mass="80786" checksum="FB24CF70B2A7C951" modified="2020-10-07" version="1">MKDMSVKIYFLLGLTITAPVQALDKKTDCPQSCSCEIRPWFNPRSIYMEALTVDCNDVGLSTFPPQLPDNTQVLLLQANSIKKIEYPQEFPVNLTGLDLSQNSLSTVATIELQKITQLLSVYLEENKLIELPDRCFFGLNNLQELYINHNLLSTIASGAFTGLDNLLRLHLNSNNLQIINSKWFEAIPNLEVLMIGENPISKIEDMNFKPLINLRSLVLAGINLREIPDNALAGLDNLESISFYDNRLIKVPHKALQKATNLKFLDLNKNPINRIQRGDFSNMIHLKELGINNMPELISIDNLAVENLPDLRKIEATNNPRLSYIHPNAFYHLPQLESLMLNSNALSALYHSTIESLPNLKEISIHSNPMHCDCVIRWINMNKTSVRFMEPESLFCVDPPEFQGQNVRMVHFREMMEICLPLIAPKSFPVNLNLETGSYVSLHCRATAVPEPEVYWITPSGHKLLPNTVSLKYYVHSEGTLEISDITQKESGLYTCIATNLVGADLKSIVINVDGPFLQENHKSFSIIVKDVRYNSILVSWKTNSKIVKSNIRWTAFPKDENSLTSQRVRIPSYQKVYNFTHLTSSMEYKICIDIPTIQLQNARRCINVTTKKLNPAMRNFEKSSINSTTILFCLGFFLGFVCVATLLGCICHEMNCDEEGHRFFRNYVKKQSFSFSELFPPLISYWDAHKEKSTALEVQATVVEVPTNMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4Z1</accession>
    <name>A0A6I9Y4Z1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">MAK</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013919363.1">
        <property type="nucleotide sequence ID" value="XM_014063888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4Z1"/>
    <dbReference type="GeneID" id="106546900"/>
    <dbReference type="KEGG" id="tsr:106546900"/>
    <dbReference type="CTD" id="4117"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000104">
        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000071">
        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="4"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013919363.1"/>
        </source>
    </evidence>
    <sequence length="567" mass="64570" checksum="C895957711EF2F35" modified="2020-10-07" version="1">MNRYTTLKQLGDGTYGSVLMGKSNESGELVAIKRMKRKFYSWDECMNLREVKSLKKLNHANVIKLKEVIRENDHLYFVFEYMKENLYQLMKDRNKLFPESVIRNIMYQILQGLAFIHKHGFFHRDMKPENLLCMGPELVKIADFGLARELRSQPPYTDYVSTRWYRAPEVLLRSSIYSSPIDLWAVGSIMAELYTLRPLFPGTSEVDEIFKICQVLGTPKKSDWPEGYQLAAAMNFRFPQCVSINLKTLIPNASSEALFLMNEMLNWDPKKRPTASQALKFPYFQVGQVLGPPPQFINQKQPYPKLTERKPSLPKLEHIPKLEAVSGPESQSSLDVTDKLQCLPKNNQHPLQSIQLPQNAVNQQPSVQQQPQTFFPAISKNSISKHIAIGPSNGAMGMKNNRRRWGQTMLKTLDSWDDFDDTEFGISYFKKPSIVVLNEKKTKESLFSVPESKSPTSGRSGENKVLKRNDSGISTTSAKQYYLKQSRYVPGVNPKNVSSGAATKERIHGTCNNPLFSKSIAPIGGLSFNQVNAEEKLIKPIEKLSCKERVNEKLEDPKGNEWHLPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5L6</accession>
    <name>A0A6I9Y5L6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Breast carcinoma-amplified sequence 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BCAS1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913245.1">
        <property type="nucleotide sequence ID" value="XM_014057770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5L6"/>
    <dbReference type="GeneID" id="106542122"/>
    <dbReference type="KEGG" id="tsr:106542122"/>
    <dbReference type="CTD" id="8537"/>
    <dbReference type="OrthoDB" id="8962384at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026115">
        <property type="entry name" value="NABC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15016">
        <property type="entry name" value="BREAST CARCINOMA-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15016:SF6">
        <property type="entry name" value="BREAST CARCINOMA-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913245.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="32384" checksum="181BD0C4E93C5ADF" modified="2020-10-07" version="1">MSFFKTLVSPSKADSKCDTEDKAKKDAPQTANTKPTEQPVPVVPVTKPETNVPPAQESPAMKPIVKPPEAPVPPPPPAVVVVTNEAPKEMTKERSSSTPTPLSKFFWKKTPTDDIEVINTERIEASPEIPVKDERRSQEVAETKSKAEERSTKTNFRKFFKLSVRSDTEVTPSEINEPAPDYQTLDFTDRPLTQAEAQDPASKGSRKNSTENLNKLQEMPQKTKDPQESRQEEAAETDSLQNGEDAAQQSPFKRLEKRQTLGEFFKGLSPKRMSDAGVQTDPVSIVSIVNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5Q8</accession>
    <name>A0A6I9Y5Q8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rab effector MyRIP-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106547397</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920018.1">
        <property type="nucleotide sequence ID" value="XM_014064543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5Q8"/>
    <dbReference type="GeneID" id="106547397"/>
    <dbReference type="KEGG" id="tsr:106547397"/>
    <dbReference type="OrthoDB" id="10072397at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15753">
        <property type="entry name" value="FYVE_SlaC2-c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000018">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051745">
        <property type="entry name" value="Intracell_Transport_Effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTIC BASIC PROTEIN MOBP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555:SF6">
        <property type="entry name" value="RAB EFFECTOR MYRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RabBD" evidence="3">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013920018.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="13202" checksum="95885CA2F3F99187" modified="2020-10-07" version="1">MRRKLDLSDLTDDETEHVIQVVQRDFTLRKKEEDRLNEIKQKLDEEGNKCSILSKHERFNEHCCMRCCSPFTFLINTKRQCQDCKYNICKNCSSYQKKENAWLCNMCQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7N3</accession>
    <name>A0A6I9Y7N3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FERM domain-containing protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915475.1">
        <property type="nucleotide sequence ID" value="XM_014060000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7N3"/>
    <dbReference type="GeneID" id="106543894"/>
    <dbReference type="KEGG" id="tsr:106543894"/>
    <dbReference type="OrthoDB" id="6266673at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17102">
        <property type="entry name" value="FERM_F1_FRMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000002">
        <property type="entry name" value="Erythrocyte protein band 4.1-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF8">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="1">
        <location>
            <begin position="33"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915475.1"/>
        </source>
    </evidence>
    <sequence length="127" mass="15363" checksum="CC2178EFCC8B70EF" modified="2020-10-07" version="1">MFAPCECVRRGGRRTMKMIRFRSSSIRSLSQEMKCTIRLLDDSEISCHIQRETKGQFLIDHVCNHYCLLEKDYFGIRYVDSEKQRHWLEPNKSIFKQMKSHPPYTMCFRVKFYPHEPLKIKEELTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y7P3</accession>
    <name>A0A6I9Y7P3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AF4/FMR2 family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AFF3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AF4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915525.1">
        <property type="nucleotide sequence ID" value="XM_014060050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y7P3"/>
    <dbReference type="GeneID" id="106543946"/>
    <dbReference type="KEGG" id="tsr:106543946"/>
    <dbReference type="CTD" id="3899"/>
    <dbReference type="OrthoDB" id="6382204at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032783">
        <property type="term" value="C:super elongation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007797">
        <property type="entry name" value="AF4/FMR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043640">
        <property type="entry name" value="AF4/FMR2_CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043639">
        <property type="entry name" value="AF4_int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528">
        <property type="entry name" value="AF4/FMR2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528:SF16">
        <property type="entry name" value="AF4_FMR2 FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05110">
        <property type="entry name" value="AF-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18875">
        <property type="entry name" value="AF4_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18876">
        <property type="entry name" value="AFF4_CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AF4/FMR2 C-terminal homology" evidence="6">
        <location>
            <begin position="579"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="431"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013915525.1"/>
        </source>
    </evidence>
    <sequence length="840" mass="93751" checksum="3DDA452E80AD5B7C" modified="2020-10-07" version="1">MKSKVLNSNVDLLFSRMLEDDLKLSSDEEESDQQAAQKSTLRVLSDSSPANQQSSCRILGVSNKGSSSSSSESDSSSESDSETESSSTESDCNKQSHCSSPEPEQSSPNKWQLDKWLNKVNPHKASALNQNGLEVFQYYSKLKEERQDNKNVSPVGQASFQEKAIKNLSKEELRSRTTNLPPGHKGRKQKTLLSAASKSVSKKQPRRTESSLAGDNLNNHAADNLDLNQIHLANNNICNQPKTRPCSSNIEQQKEPQSVTAVEKRRTRGSGKTAPKSKEFIETESSSSTSSDTESQSEQEECPSHKLQTVASASAGSDQKQKNSSNNNGHKKTNNCGAFGSINTRTSNDIAKELEEQFYTLVPFGRNELLSPLKDTDDVKALWVKIDLTLLSRIPRQLPEEPLIMNTGVKEAIQMQYNIAADLPAEKVIPKSRRKRKYENEEENRETKKIHTEQKCSSNLLPSAQVISSPNHYKTIKKSLATPINKNENMLQSPSSPFSDASKYKFCNDDLTSSKTNGSNPSSSTSSSRKHKNEIRTYLYPGDFSKAMYISSENTLFCNRTQCQKESWSSILTVPKICRKSKLIVNNGLHNLDYFMQEAKRKKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPLESKSPYTMYAETVELIRCVSDNTPTIDWLLCSYRIQMCFLSFPYSYRCLALLYWRMFQLKRDHAVKYSKALIEYFKNSSKVSQAPSTWSGSVKCTGTLSPISPSLSPLSSVGSTGAPSPSSIISIPQRIHQMAANHVSITNSILHSYDYWDMADNLGKENQDFFNDLDALMGAITLHSSMEHLVQYTHQGLDWIRCSAQLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8C0</accession>
    <name>A0A6I9Y8C0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endogenous retrovirus group 3 member 1 Env polyprotein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106544796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013916660.1">
        <property type="nucleotide sequence ID" value="XM_014061185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8C0"/>
    <dbReference type="GeneID" id="106544796"/>
    <dbReference type="KEGG" id="tsr:106544796"/>
    <dbReference type="OrthoDB" id="9950230at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026999690" evidence="1">
        <location>
            <begin position="29"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916660.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="20440" checksum="C8D934CAF21C14EF" modified="2020-10-07" version="1">MQPTFTLAQQLVLMLFILGISTPKAVVSQSQCEQCLVTTHHGNKVTKTFVYHSHYNCKEQTTSCVHNTITYEICPNNGNPICFNPQGSQFALDVEITGSQEGGVAQEGGVCRKCNPNNRCLQFDDSLKVVEESATRIRKLTHVPVQIWGGAVDFNSFGGIFSGWKRIGFLVLLALEVSFSSPVSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8J5</accession>
    <name>A0A6I9Y8J5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitogen-activated protein kinase kinase kinase kinase 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106545097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013917035.1">
        <property type="nucleotide sequence ID" value="XM_014061560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8J5"/>
    <dbReference type="GeneID" id="106545097"/>
    <dbReference type="KEGG" id="tsr:106545097"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CNH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013917035.1"/>
        </source>
    </evidence>
    <sequence length="273" mass="30350" checksum="B182A516D10BFF24" modified="2020-10-07" version="1">MGNVLMSLSGKVPQLFSHNLLGLHEQSKRDQRLGVSLSPHRLLPRKNVITTKIPDTKGCQKCCTARNTQNSCHFLCGALEAGVVLLQWYEPMQKFMLVKYFDFPLPSLLPIFEMLINPEEEYPVVCIGVSKGPPGQAVQFQVINLNSLTSWFISDKSEPSCCGFVQVTQLDNRTVMVLMDGCIQFVSPRGALLGSPPKLTFDVAVESVALVQHCVHAFWRHGVQTRPLGSGQVAQKLQDQRWTFRLLGASSTIVLETRPVNNPNSTSNLYVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8S5</accession>
    <name>A0A6I9Y8S5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclosome subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CDC23</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the APC8/CDC23 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917435.1">
        <property type="nucleotide sequence ID" value="XM_014061960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8S5"/>
    <dbReference type="GeneID" id="106545410"/>
    <dbReference type="KEGG" id="tsr:106545410"/>
    <dbReference type="CTD" id="8697"/>
    <dbReference type="OrthoDB" id="10262026at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000093">
        <property type="entry name" value="cell division cycle protein 23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007192">
        <property type="entry name" value="APC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF10">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04049">
        <property type="entry name" value="ANAPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 14" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cdc23" evidence="12">
        <location>
            <begin position="27"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="327"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="361"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013917435.1"/>
        </source>
    </evidence>
    <sequence length="594" mass="68489" checksum="E6F8959BD5A863B5" modified="2020-10-07" version="1">MAAGASVSGPTAAGADFACADFCDLREIKKQLLDVAERSRERGLQHSGKWASELAFALDPLPLNELPPVPELTEEDACDLDAYTLAKSYFDLKEYDRAAYFLRNCKSPKAYFLYMYSRYLSGEKKKDDETVDSLGPLEKGQVKNEVLRELRVELSKKHKARELDGFGLYLYGVVLRKLDLVKEAIDVFVEATHILPLHWGAWLELCNLITDKETLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQSLIDVGFSKSTYIISQIAVAYHNIRDIDKALSIFNELRKQDPFRIENMDTFSNLLYVRGMKPELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLNESEQAAQCYIKYIQDIYSCGEIGENLELSTAFRYLAQYYFKCKLWDEASVCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTETAATPFLLPSSLSASNTPTRRVSPLNLSNITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB36</accession>
    <name>A0A6I9YB36_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sperm-associated antigen 16 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106548445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921328.1">
        <property type="nucleotide sequence ID" value="XM_014065853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB36"/>
    <dbReference type="GeneID" id="106548445"/>
    <dbReference type="KEGG" id="tsr:106548445"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990716">
        <property type="term" value="C:axonemal central apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050995">
        <property type="entry name" value="WD-F-box_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF3">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013921328.1"/>
        </source>
    </evidence>
    <sequence length="274" mass="32149" checksum="F5E56B0E60BB971A" modified="2020-10-07" version="1">MEEATTTEETFCLQQVTITESSEDDYQYEEIPADEDFSLQEGDEDLAKALHTMHEQTLDAQIMAQRPGQITKQSLSETPEIVDDFLCNFLIRMGMNRTLDCFQTEWYELMQRGVLEPKDVGFVPDAYTCNQHLERENIYLKKEMENYKFAANKAKDALLKMQRERDFHRMHHKRVVQEKNRLINDIKRLKTHYAMYEPMLKQLNEKYEILLREKMLTSLERDKAVGQITGLQATLQSLESRNNLHIPVMKVGHWCDREAGLVGPSQRALREAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB78</accession>
    <name>A0A6I9YB78_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cholesterol 25-hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CH25H</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921362.1">
        <property type="nucleotide sequence ID" value="XM_014065887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB78"/>
    <dbReference type="GeneID" id="106548506"/>
    <dbReference type="KEGG" id="tsr:106548506"/>
    <dbReference type="CTD" id="9023"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="136"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921362.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="33155" checksum="816C349C3C362C6F" modified="2020-10-07" version="1">METHTHNGSSGLPFCNQPNGLPDPPALQPLWDSVLRREGLLKSPLFPALFNFAVYTAFCSPFVALDLLSPWLPFLRRYKIQPQSSPTPQMAWLCLAQSIYHHVVFIFPVTVAHWYWRPVSLPRMAPEVPTLLLQVAACLLLFDFLSFLWHLLHHKVPWLYKNFHKVHHKHISTFALTTQYASVWELVWLGFFTVVGPMLLRCHPLTEMTFFIVNIWLSVEDHSGYDLPWSTHRLVPFGLYGGAPHHDLHHLKFKFNYAPYFTHWDRLFGTLSHSAKPREVKVNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBF8</accession>
    <name>A0A6I9YBF8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Inositol polyphosphate 1-phosphatase</fullName>
            <ecNumber evidence="12">3.1.3.57</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">INPP1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mg(2+)-dependent phosphatase that catalyzes the hydrolysis of the 1-position phosphate from inositol 1,4-bisphosphate and inositol 1,3,4-trisphosphate and participates in inositol phosphate metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1D-myo-inositol 1,3,4-trisphosphate + H2O = 1D-myo-inositol 3,4-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:70319"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:83241"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:70320"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1D-myo-inositol 1,4-bisphosphate + H2O = 1D-myo-inositol 4-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15553"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58282"/>
            <dbReference type="ChEBI" id="CHEBI:58469"/>
            <dbReference type="EC" id="3.1.3.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:15554"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Signal transduction; phosphatidylinositol signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.57" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013921452.1">
        <property type="nucleotide sequence ID" value="XM_014065977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBF8"/>
    <dbReference type="GeneID" id="106548583"/>
    <dbReference type="KEGG" id="tsr:106548583"/>
    <dbReference type="CTD" id="3628"/>
    <dbReference type="OrthoDB" id="9977309at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000015">
        <property type="entry name" value="Inositol polyphosphate 1-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.460.10:FF:000001">
        <property type="entry name" value="Inositol polyphosphate 1-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.460.10">
        <property type="entry name" value="Inositol Polyphosphate 1-phosphatase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044897">
        <property type="entry name" value="INPP1_dom_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF3">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 1-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0452">Lithium</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="15">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013921452.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="40642" checksum="E027E1444AF8661B" modified="2020-10-07" version="1">MTALLRSLLAASEKAACIAQLCRQEEALFSLLIEEKRGADKNKKFLQDFKTLADVLIQEVIKHDVGKEFPELQDHICGEESNKFENGLGEIVVVQVCPTQQETAALLQKVLDRKQTAAELLAAAVHQEVVLSDPALDNVDVTISTESLAVWIDPIDSTNQYIRGCSSVMPVNGIYPSGLHSALVLIGAYDRHSGEPVLGIINEPFFQEELTPHRWQSKYHWGICYGSTKLSSLSQPQQRSSPCVVLSSNGTGTLQKTLAPLYGERFCFASGAGYKMLCVALGLADAYVLSEGSTFKWDSCGPHAILRALGGGIVDLSAALRLWRAGQRKNLPELTYNKPIEGAVGADRWANQGGLIAYTCHKHLEAVMATLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCD1</accession>
    <name>A0A6I9YCD1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Keratin, type I cytoskeletal 10-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106548838</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921793.1">
        <property type="nucleotide sequence ID" value="XM_014066318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCD1"/>
    <dbReference type="GeneID" id="106548838"/>
    <dbReference type="KEGG" id="tsr:106548838"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF379">
        <property type="entry name" value="IF ROD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="139"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="250"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="535"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013921793.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="63178" checksum="4C4B4D64A10B3EFA" modified="2020-10-07" version="1">MSCSTKGGGSSVGGGRICGGSSIKITSSGSSRGLSGKSYGGSRSGYGGGGFSSGSCGRISRGRISYGGSCYGGGSYGGGSYGGGSYGGHGGSGYGGVCYGGGSYGGGSYGGGSYGGGSGYPFSSGSFSGGDGGVFSSNEKATMQNLNDRLANYLDQVKRLEDENCQLEIWIKEWYQKHGDIGPDKDYSHYYQEIDQLYNELIAETDDYNKILLNIDNTRMTAEDFKLKYNTEAGLRQNVEADINGLRPLLDQLTLAKSELEMQFESLKEELICLKKNHEETLRGLRGQRGHGDVSVEVNAAPGQDLKQRLDQLRCEYENIIEQNRKEVEAWYESKMEEVRQEVTSSDQEISSSNHQLSELRREYQTLEIELQSQISLIQSMQTNLEDTERRYNMQLQQIQNMIEPVEGELASIRCEIESQNQEYQLLLGIKTRLEQEICQYRRLLEEGQQEIGSGGYGGGHGGGRRGGGGISGGGVCVGGGVSGGSLGGGGVCVGGGVSGGSISGGSHSGGGVCIGGGIGGGHIGGGISSRPQCYPTSSHSQGGKTDSQDPINMLLQVVPERYAEKEMKLLKWNADHSSPKRFRAENKTEIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD42</accession>
    <name>A0A6I9YD42_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-2-macroglobulin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922086.1">
        <property type="nucleotide sequence ID" value="XM_014066611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD42"/>
    <dbReference type="GeneID" id="106549062"/>
    <dbReference type="KEGG" id="tsr:106549062"/>
    <dbReference type="OrthoDB" id="9049374at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF165">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="1">
        <location>
            <begin position="53"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922086.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="16951" checksum="A60AA861B1BAC481" modified="2020-10-07" version="1">MKIPLKYNVPLRQEEAPFWLEVDTVPETCTGVKAHVTFDIAINVSYTGQRLVSNMAIVQIKMLSGYIPVKSSVKKLEKESQIKRTEVNINHVLLYLDEVPKTVQTFSFTVEQETPVWDLQPALVKIYDYYETDEFATAEYTAPCSTGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD49</accession>
    <name>A0A6I9YD49_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">F-box/WD repeat-containing protein 11 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FBXW11</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921984.1">
        <property type="nucleotide sequence ID" value="XM_014066509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD49"/>
    <dbReference type="GeneID" id="106549005"/>
    <dbReference type="KEGG" id="tsr:106549005"/>
    <dbReference type="CTD" id="23291"/>
    <dbReference type="OrthoDB" id="19711at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22183">
        <property type="entry name" value="F-box_FBXW1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000001">
        <property type="entry name" value="F-box/WD repeat-containing protein 11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000004">
        <property type="entry name" value="F-box/WD repeat-containing protein 11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056829">
        <property type="entry name" value="Beta-prop_TEP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021977">
        <property type="entry name" value="Beta-TrCP_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050995">
        <property type="entry name" value="WD-F-box_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF6">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN-CONTAINING 11-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25047">
        <property type="entry name" value="Beta-prop_TEP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12125">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01028">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="150"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="257"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="297"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="337"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="380"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="420"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="460"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="509"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921984.1"/>
        </source>
    </evidence>
    <sequence length="563" mass="64710" checksum="437F890105DCC02A" modified="2020-10-07" version="1">MEPESVIEDKTIELMCSVPRSLWLGCSNLVESMCALRCLQSMPSVRCLQNTSVMEDQNEDESPKKNTLWQISNGTSSVIVSRKRPSEGNYEKEKDLCIKYFDQWSESDQVEFVEHLISRMCHYQHGHINSYLKPMLQRDFITALPEQGLDHIAENILSYLDARSLCAAELVCKEWQRVISEGMLWKKLIERMVRTDPLWKGLSERRCWDQYLFKNRPTDGPPNSFYRSLYPKIIQDIETIESNWRCGRHNLQRIQCRSENSKGVYCLQYDDEKIISGLRDNSIKIWDKTGLECLKVLTGHTGSVLCLQYDERVIVTGSSDSTVRVWDVNTGEVLNTLIHHNEAVLHLRFSNGLMVTCSKDRSIAVWDMASATDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWSTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLQAALDPRAPASTLCLRTLVEHSGRVFRLQFDEFQIISSSHDDTILIWDFLNVPPNAQNETRSPSRTYTYISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF16</accession>
    <name>A0A6I9YF16_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="9">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GRK5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_013922639.1">
        <property type="nucleotide sequence ID" value="XM_014067164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF16"/>
    <dbReference type="GeneID" id="106549495"/>
    <dbReference type="KEGG" id="tsr:106549495"/>
    <dbReference type="CTD" id="2869"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF27">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013922639.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="29626" checksum="F62346BFFD1B1147" modified="2020-10-07" version="1">ILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDSIRGRVGTVGYMAPEVLNNQRYTLSPDYWGLGCLIYEMIAGQSPFRGRKEKVKREEVDRRVLEMEEVYSHKFSEEAKSICKMLLLFLFVLGQPRAVYCKDVLDIEQFSTVKGVNLDQTDDDFYSKFSTGSVSIPWQNEMIETECFKELNLFGPNGTISPDLNKNYPPEPPKKGLLHRIFKRQHQNNSKSSPNSKTNLNHHINSNHVSSNSTGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGE1</accession>
    <name>A0A6I9YGE1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine-rich PDZ-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cysteine-rich interactor of PDZ three</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CRIPT</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRIPT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923338.1">
        <property type="nucleotide sequence ID" value="XM_014067863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGE1"/>
    <dbReference type="GeneID" id="106550077"/>
    <dbReference type="KEGG" id="tsr:106550077"/>
    <dbReference type="CTD" id="9419"/>
    <dbReference type="OrthoDB" id="147332at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019367">
        <property type="entry name" value="PDZ-binding_CRIPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805:SF1">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10235">
        <property type="entry name" value="Cript"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013923338.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="11240" checksum="9B9F291883F8ECD7" modified="2020-10-07" version="1">MVCEKCERKLGTVITPDTWKDGARNTTESGGRKLNENKALTSKKARFDPYGKNKFAICRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGE7</accession>
    <name>A0A6I9YGE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NOP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923074.1">
        <property type="nucleotide sequence ID" value="XM_014067599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGE7"/>
    <dbReference type="GeneID" id="106549846"/>
    <dbReference type="KEGG" id="tsr:106549846"/>
    <dbReference type="CTD" id="4839"/>
    <dbReference type="OrthoDB" id="427002at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000001">
        <property type="entry name" value="Ribosomal RNA methyltransferase Nop2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023273">
        <property type="entry name" value="RCMT_NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054728">
        <property type="entry name" value="RsmB-like_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22458">
        <property type="entry name" value="RsmF-B_ferredox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02012">
        <property type="entry name" value="RCMTNOP2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="11">
        <location>
            <begin position="291"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="583"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="637"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="683"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="383"/>
            <end position="389"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013923074.1"/>
        </source>
    </evidence>
    <sequence length="696" mass="77622" checksum="DFEB16B2E34B64BB" modified="2020-10-07" version="1">MGRKLDPTRQVKRGPGRKARKQRGAEEELARFLPPALDDNAAKLSSHARRRAAKRRVKSEQLQKNKNAPNHQNVTAKKKTLKNAKKGKSSPEDNPTNGSTDAAMQLPSPALRKKRSRTEESEEDKEESMAFSDEDDLSEDEIRDDYGVASSSDEELLPIERAARKQKMEGQDISEDDSEDETPPSSQRIEQKEEKDADLQLNMEAEECFVLPSGQEIEKETAQPPDLQLIHQRIKDNMEVLQNFSSKREEGRSRQEYLSLLHQDLAAYYSYSDFLIGKMTELFPLSELVEFLEANEVSRPVTLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKTGLVVYDSSVPIGATPEYLAGHYMLQGASSLLPVMALAPQENERILDMCCAPGGKTSYIAQLMKNTGVILANDSNSERLHSVVGNLHRLGVTNTVVSNCDGRQFPKVLGGFDRVLLDAPCSGTGVISKDPTVKTNKDEKDILRCAHLQKELILSAIDSVNAASETGGYVVYCTCSVMVEENEWVVDYALKKRNVRLVPTGLDFGKEGFTRFRERRFHPSLKSTRRFYPHTHNMDGFFVAKLKKFSNAIPKPQKDEVPSEELTMQTVKNGSAPTEPPPKKKKDAESKASPQQASKQELSAAVAARKEAKGELKASKKKKALAASKQSNFQKKSRLLNAAWPSPGGLRAKSTPRKKGRVQRNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGN2</accession>
    <name>A0A6I9YGN2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Suppressor of cytokine signaling 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">SOCS5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923336.1">
        <property type="nucleotide sequence ID" value="XM_014067861.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013923337.1">
        <property type="nucleotide sequence ID" value="XM_014067862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGN2"/>
    <dbReference type="GeneID" id="106550076"/>
    <dbReference type="KEGG" id="tsr:106550076"/>
    <dbReference type="CTD" id="9655"/>
    <dbReference type="OrthoDB" id="8820570at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03739">
        <property type="entry name" value="SOCS_SOCS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000002">
        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000028">
        <property type="entry name" value="Suppressor of cytokine signaling 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022252">
        <property type="entry name" value="SOCS4/SOCS5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037343">
        <property type="entry name" value="SOCS5_SOCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF15">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12610">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="372"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="462"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013923336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923337.1"/>
        </source>
    </evidence>
    <sequence length="527" mass="60162" checksum="4727A975247B99E8" modified="2020-10-07" version="1">MGKVGKMWNSFKYRCQNLFNPEGGNRNENVDINSNSASVRDKSVCVPGATQQQPNSPLQLSLNTSKGFSRKNQNCATDIPQIVEISIEKEIDLNVASGAKFARRDSYSRHAPWGGKKKHSCSTKTQSSLESDTRFGCSHSQRRERRYGVSSVNDTDSISSRTIGNRSLRQRLQETVGLCFPMRTYCKQSKPLFSNKRKIHLSELMLEKCPFPAGSDLAQKWHLIKQHTAPVSPHSAIFDAFDSSLASTEDEEDRLRERRRLSIEEGVDPPPNAQIHTFEATAQVNPLYKLGPKLPPGMSELMGDCNSTTQGSCDLEEDTTTLCLQSRRQKQRQISGESHSHISRQGAWKVHTQIDYIHCLVPDLLQITSNPCYWGVMDRYEAEALLEGKPEGTFLLRDSAQEDYLFSVSFRRYNRSLHARIEQWNHNFSFDAHDPCVFHSSTVTGLLEHYKDPISCMFFEPLLTVSLNRTFPFSLQYICRAVICRCTSYDGIDCLPLPTMLQDFLKEYHYKQKVRVRWLEREPIKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGS2</accession>
    <name>A0A6I9YGS2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein KIAA0825 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KIAA0825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923387.1">
        <property type="nucleotide sequence ID" value="XM_014067912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGS2"/>
    <dbReference type="GeneID" id="106550116"/>
    <dbReference type="KEGG" id="tsr:106550116"/>
    <dbReference type="CTD" id="285600"/>
    <dbReference type="OrthoDB" id="10007406at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027993">
        <property type="entry name" value="DUF4495"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33960">
        <property type="entry name" value="SIMILAR TO KIAA0825 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33960:SF1">
        <property type="entry name" value="SIMILAR TO KIAA0825 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14906">
        <property type="entry name" value="DUF4495"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013923387.1"/>
        </source>
    </evidence>
    <sequence length="627" mass="72131" checksum="8F81DAEF7A73FACD" modified="2020-10-07" version="1">MEWRDDFSLDSLNLDCLLNTFPGNLEVQQVLGDIDEKIKKNTSCVDQCLKELQLELNETCSDELLQDTTSFLHWLNNHDFSSTKFSSTNQGELIKFLQTLQRLLKNEQNQEEIILQFLLELSNQCGVLFPCTPSGASFEFVSSSSIHGIEDDTAVDVHCIWDDIRLHLRRYLVHQLQSNQKIKTGTLQHQLQFKTQCLQQLLFLYPESEVLVKYQKMQKKLVSDLLQKCAQGKSDEMNFEKIVHNYNSSIPAFCAMIKEDLYILSGIIDSSSVLKFINETYLDTFTEEITFVFHMLCQLQLKEKALHDIKTSKNSKKNRGMVQVVVAEEHSRKGMNQCLTLHQLKCLSQLIKSCLLMEENIEELSSGLLLVCMSENKSIQGVLKNTCGELFTGANESSKFPEHILKVKKSAMLEFGWRNVFKDLSSSVAHSLIIAVEDFSGKILEHEQNEQCSATYYMMCLVNVQNMNDSWRVVNEKEQPKQISKFCSDVMEVLDMLLPLALACRVEVFQEIRANFIEACSKVAAALLTRLEERSKDVPTRAPLQNLYTILSTAVHVYQRFKMYQNVMKVESGAPLFLALLQQYKELISILQFQVTNYCIQVCITSILQDAESHYWEDKKAFYEVWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHW4</accession>
    <name>A0A6I9YHW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Extracellular calcium-sensing receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">CASR</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="18">Homodimer; disulfide-linked. Interacts with VCP. Interacts with ARRB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923948.1">
        <property type="nucleotide sequence ID" value="XM_014068473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHW4"/>
    <dbReference type="GeneID" id="106550537"/>
    <dbReference type="KEGG" id="tsr:106550537"/>
    <dbReference type="CTD" id="846"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005513">
        <property type="term" value="P:detection of calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051924">
        <property type="term" value="P:regulation of calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="7tmC_CaSR"/>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="19">
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            <begin position="820"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="924"/>
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    <sequence length="973" mass="108457" checksum="34AF8FF5D0895140" modified="2020-10-07" version="1">MTFYSFCLILFALTWSSSAYGPNQRAQKKGDIILGGLFPIHFGVAAKHQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINNSPTLLPNMTLGYSSFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSDHIPSTIAVVGATGSGISTAVANLLGLFYIPQVSYASSSRLLSNRNEYKSFLRTIPNDEHQATAMADIVEYFRWNWVGTIAADDNYGRPGIEKFREETEERNICIDFSELIAQYLPEDQIQQVVKVIQNSTAKVIVVFSSAPDLEPLIKEIVTRNITGRIWLASEAWASSSLIAMPQFFHVMGGTIGFALKAGQIPGFRDFLQKVNPKKSANNGFIKEFWEETFNCYLPEGNKNFPGFFHKGYEEGPRAGNGTASFRPPCTGRENITSVETPYMDYTHLRISYNVYLAVYSIAHALQDIYTCTPGKGLFANGSCADIKKVEAWQVPFSNCSRDCLPGTRKGIIEGEPTCCFECVECTDGEISDETDASACEKCAENFWSNENHTFCIPKQIEFLSWTEPFGIALTLFAVLGVFLTSFVLGVFTRFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWNCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSLHRKWWGLNLQFLLVFLCTFVQIVICVIWLYTAPPSSFRNHEMEDEIIFITCNEGSLMALGFLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRCSTAAHAFKVAARATLRRSNVSRKRSNSLGGSSGSTPSSSISSKSNHEEPFSIPTSVEPQQKHGCKQKVSFGGGTVTLSLSFEEHQKNAIANKNAKHRNSLEARNSDDSLMRHKALLPLHGNESLEPKFQIPSNQESSIPESVVGETKQGEHVEGPPSLSAAYLRDLVGSGSIKENAIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHZ0</accession>
    <name>A0A6I9YHZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">GPI-anchor transamidase component GPAA1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GAA1 protein homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Glycosylphosphatidylinositol anchor attachment 1 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">GPAA1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the glycosylphosphatidylinositol-anchor (GPI-anchor) transamidase (GPI-T) complex that catalyzes the formation of the linkage between a proprotein and a GPI-anchor and participates in GPI anchored protein biosynthesis. Binds GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heteropentamer. Part of the GPI-anchor transamidase complex, consisting of PIGK, PIGT, PIGS, PIGU and GAA1. Interacts with PIGK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923584.1">
        <property type="nucleotide sequence ID" value="XM_014068109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHZ0"/>
    <dbReference type="GeneID" id="106550242"/>
    <dbReference type="KEGG" id="tsr:106550242"/>
    <dbReference type="CTD" id="8733"/>
    <dbReference type="OrthoDB" id="445301at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000047">
        <property type="entry name" value="Glycosylphosphatidylinositol anchor attachment 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304:SF0">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036762">
        <property type="entry name" value="GAA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="505"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="545"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="601"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013923584.1"/>
        </source>
    </evidence>
    <sequence length="624" mass="69350" checksum="1A723234E4B74FF7" modified="2020-10-07" version="1">MGLLSDPHWRRALSRLLLRLHAPLCALSFVAGLGWFLGLAFQPLALRAAMSENAMGSTMVEERFARGDRALALARDFAAQRKKAGGALPVAWLEKSLRALGLEVYAQSFSRTLPFPDEARERYAVKGTNVYGILRAPRAASTESLVLSVPCSDGQPRNSQAVGLMLALAAHFRGQVYWAKDIIFLVNEHDLIGMEAWLEAYHDVNVTETHSSGTFGRAGAIQAAISLELSSDVITSFDVAVEGLNGQLPNLDLVNLFYSFCQKNGLLCTIQGKLQRSDWDSLPAYLHSLQTLLLMVLKQGSGHPQGDHGLFLRYHIEAITIRGINSFRQYKFDMGVMGVTFEGIFRKLNNLLERLHQSYFFYLLPSLSRFVSIGLYMPAFGFLILILVLKALDLWVKLSSFDSDRSQLCDGDQASSPAPVEDQRPSVLTLIPPLLICHATGLALYFMPVWGQKVATEHFPVSESEAVVLTSIGIYVAGLALPHNTHRVLMGSGSNQGWMMLKLFALLYLAMQLSCVALINFSLGFLLTVTMAPVVAIVQPTGPRYLYAGLLLLVTPAVTLLLCIFLYQELMEYPISPLEGWQRFLQVIAEGLLDHYLYGSIVFPFVAIFVYPCWLLFWNVLFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIV5</accession>
    <name>A0A6I9YIV5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Akirin-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AKIRIN2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the akirin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923884.1">
        <property type="nucleotide sequence ID" value="XM_014068409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIV5"/>
    <dbReference type="GeneID" id="106550484"/>
    <dbReference type="KEGG" id="tsr:106550484"/>
    <dbReference type="CTD" id="55122"/>
    <dbReference type="OrthoDB" id="10039914at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22244">
        <property type="entry name" value="akirin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024132">
        <property type="entry name" value="Akirin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13293:SF8">
        <property type="entry name" value="AKIRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13293">
        <property type="entry name" value="AKIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013923884.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21603" checksum="128E80BACF679BB1" modified="2020-10-07" version="1">MACGATLKRTLDFDPLLSPASPKRRRCTPLSASAAASSSSASFASSPQKYLRMEPSPFGEVSTRLTTEQILYNIKQEYKRMQKRRHLENFHQTDPCCSTDAQSQAFLLSGPALPGTSSAASPPLKKEQPLFTLRQVGMICERLLKEREEKIREEYEEILTTKLAEQYDAFVKFTHDQIMRRYGEQPASYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIY1</accession>
    <name>A0A6I9YIY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TRIM3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013924313.1">
        <property type="nucleotide sequence ID" value="XM_014068838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIY1"/>
    <dbReference type="GeneID" id="106550855"/>
    <dbReference type="KEGG" id="tsr:106550855"/>
    <dbReference type="CTD" id="10612"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20482">
        <property type="entry name" value="CC_brat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14960">
        <property type="entry name" value="NHL_TRIM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16768">
        <property type="entry name" value="RING-HC_TRIM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000007">
        <property type="entry name" value="Putative tripartite motif-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000004">
        <property type="entry name" value="Tripartite motif containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000032">
        <property type="entry name" value="Tripartite motif containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000154">
        <property type="entry name" value="Tripartite motif-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057750">
        <property type="entry name" value="TRIM2/3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF21">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="16">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="12">
        <location>
            <begin position="320"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="13">
        <location>
            <begin position="473"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="13">
        <location>
            <begin position="522"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="13">
        <location>
            <begin position="564"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="13">
        <location>
            <begin position="609"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="13">
        <location>
            <begin position="656"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="13">
        <location>
            <begin position="700"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="423"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013924313.1"/>
        </source>
    </evidence>
    <sequence length="744" mass="81533" checksum="BD284E584B06FB60" modified="2020-10-07" version="1">MAKRESSSSPVVRQIDKQFLVCSICLDRYHNPKVLPCLHTFCERCLQNYIPPQSLTLSCPVCRQTSILPEKGVAALQNNFFITNLMEVLQRQPDNASHEDAIAMLDSVSAVPGKPLSCPNHEGKTMEYYCEACETAMCHECTVGEHREHVTVPLRDVVEQHKASLRQQLDAIKSRLPQLAAAVELVSGISQQLAERKNDAVAEIGSTFTELEKALGQRKGLLVRDLEALCGAKQKVLQAQLEVLRQGQENILSSCSFTEQALDHGSEMEVLLVKKQMSERLSELASREFPEQPHENAQVDYVVETEGVRKSILNLGVLLTTSAIAHKTVATGEGLRHAVVGQAASLTITTKDKDGELVRNGSACLQAEVVAPDNCALEHEVQDNKNGTYELLYTPRQEGDHVLSICLYGQPIRSSPFRVKAVKASDVPPSPDDVKRRVKSPSSGHIRQKAVRRPSSMYSSGKKKENPIEDELIFRVGSRGREKGEFTNLQGISTSSSGRIVVADSNNQCVQVFSNEGQFRLRFGIRGRSPGQLQRPTGVTVDLNGDIIIADYDNRWVSIFSPEGKFKTKIGAGRLMGPKGVAVDRNGHIIVVDNKACCVFIFQSNGKLVAKFGSRGTSERQFAGPHFVAVNNKNEIVVTDFHNHSVKVYSADGEFLFKFGSHGEGNGQFNAPTGVAVDSNGNIIVADWGNSRIQVFDSSGSFLSYINTTADPLYGPQGLALTSDGHVVVADSGNHCFKAYRYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKK3</accession>
    <name>A0A6I9YKK3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 134</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCDC134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924280.1">
        <property type="nucleotide sequence ID" value="XM_014068805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKK3"/>
    <dbReference type="GeneID" id="106550824"/>
    <dbReference type="KEGG" id="tsr:106550824"/>
    <dbReference type="CTD" id="79879"/>
    <dbReference type="OrthoDB" id="5854099at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026321">
        <property type="entry name" value="CC134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14735">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14735:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15002">
        <property type="entry name" value="ERK-JNK_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924280.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="27894" checksum="316D78F6BA3D6938" modified="2020-10-07" version="1">MVVDSGSSWILRMDYFVACTFLFTLILPRGMTTDTEKKTSDSGLEIYKKLFEVKRKDQMNALKNLIELNDVNQQYKIIDIMLKGLFKVLEDSRAVLIAADISPDGPFSHDEKIKDAYSHVVENTAFFGDVVLRFPKIVHHYFDRNSNWNNLIRWGISFCNQTGVFDQGPHSQVLGLMAQELGISERSPDYQNPFKADNSEFFPGDSTFQKALREEEKRRKKEEKRKEIRKGPRISRSQSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLL5</accession>
    <name>A0A6I9YLL5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cadherin-18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106551532</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925127.1">
        <property type="nucleotide sequence ID" value="XM_014069652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLL5"/>
    <dbReference type="GeneID" id="106551532"/>
    <dbReference type="KEGG" id="tsr:106551532"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF106">
        <property type="entry name" value="CADHERIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026674400" evidence="6">
        <location>
            <begin position="25"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="79"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="160"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925127.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="30206" checksum="A0747D1F6FF942DA" modified="2020-10-07" version="1">MKITSTSCICPVLVCLCFVQRCYGTAHHGSIKVTRNQTKHIEGETEVHHRPKRGWVWNQFFVLEEHMGPDPQYVGKLHSNSDKGDGSVKYILTGEGAGTIFIIDDTTGDIHSTKSLDREKKTHYVLHAQAIDRWTNKPLEPESEFIIKVQDINDNAPKFTDGPYIVTVPEMSDMGTSVLQVTATDADDPTYGNSARVVYSILQGQPYFSVDPKTGVIRTALHNMDREAREHYSVVIQAKDMAGQVGGLSGSTTVNITLTDVNDNPPRFPQSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLY1</accession>
    <name>A0A6I9YLY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WD repeat-containing protein 89</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">WDR89</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925398.1">
        <property type="nucleotide sequence ID" value="XM_014069923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLY1"/>
    <dbReference type="GeneID" id="106551771"/>
    <dbReference type="KEGG" id="tsr:106551771"/>
    <dbReference type="CTD" id="112840"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039328">
        <property type="entry name" value="WDR89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="168"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013925398.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="41819" checksum="B5030273A04ED692" modified="2020-10-07" version="1">MSVAMEKIKEQFSSLQIAKRCSLPEDPSYLLDIDISRAEGSNLVAVSCSNTSIRVYNRETLCLLQEYSSPPALLSDVRFAHTNENILFSAGSDGSLKCWDIRSSAVKPAQLFSGSPSNVFISCDISCNDLVVCAGTEKVKEDAFIIFWDARGCPNSASSSKKPLGVYSESHNDDVTKVCFHPNKPHLLASGSTDGLVNVFDINKDKEENALVSTCNSDSSVSFIGWAGKDYWQVYCMTHDEGFCYWDLAELDSEKSTTLLHIPDVREVENIKLDYLIGGVYHEQVDKLFAFGGMSTGSICLVECCAQGLNSVGILQGGHSATVRSFCWNMVDDSLFTGGEDAELLLWKPGAVEKAKKTSLKAASSVNQQTKVHQHSLQTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMH9</accession>
    <name>A0A6I9YMH9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">HIVEP1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with UTP4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925437.1">
        <property type="nucleotide sequence ID" value="XM_014069962.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551807"/>
    <dbReference type="KEGG" id="tsr:106551807"/>
    <dbReference type="CTD" id="3096"/>
    <dbReference type="OrthoDB" id="10042249at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000033">
        <property type="entry name" value="Immunodeficiency virus type I enhancer binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000083">
        <property type="entry name" value="Immunodeficiency virus type I enhancer binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001151">
        <property type="entry name" value="zinc finger homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051969">
        <property type="entry name" value="Zinc-finger_DNA-bd_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034729">
        <property type="entry name" value="Znf_CCHC_HIVEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944">
        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51811">
        <property type="entry name" value="ZF_CCHC_HIVEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC HIVEP-type" evidence="16">
        <location>
            <begin position="946"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="2041"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="2069"/>
            <end position="2098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="25"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="218"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="274"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="489"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="638"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="891"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1012"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1382"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1424"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1481"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1770"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1817"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2122"/>
            <end position="2186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2217"/>
            <end position="2257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2286"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2547"/>
            <end position="2678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="638"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="658"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="891"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1382"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1428"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1786"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="2129"/>
            <end position="2151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="2152"/>
            <end position="2178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="2241"/>
            <end position="2253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="2570"/>
            <end position="2595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="2616"/>
            <end position="2625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013925437.1"/>
        </source>
    </evidence>
    <sequence length="2678" mass="292597" checksum="34BDABDB73D0C7A3" modified="2020-10-07" version="1">MNEKNYCIEEGFVCFDKIEEAQKELNETEDSKKDISKAITRGSTDTVKGVKRKKIVAENHQTKIPKSPLRTPTQAKSKEKVEDHSASSKTLPDTSELLKESPSLASQNGKQNKQNVGTFNSEVAVKVSTAETILHTKPPLLPQSLDLNKWPVEEYSSTQLHLPVKKGLTSCSSISKIDNNECISFVDCNNLSSCTNTAFDVLLKAMEPELNTLAHECPSSGMQIDKQRPNRTVNTSSSLASNTRSAQSHEASLQQKNVAASQFYPCTPQTVHVTTSGKTSQAQTHSAAHSKEQVPAKAGPRNQQVTACPNFTSSVVHQQNQENLKLQHIYSTAVTSSVTSQSSVSQAFCQNHLVAKSPSPLSINPALLPSSTAQVPIAPLYNSAQIASVVNPGSEQICNLLKVQKPKKMGKYVCEYCNRACAKPSVLLKHIRSHTGERPYPCETCGFSFKTKSNLYKHKKSHAHAIKVGRVLQPDSSGMFLSHESDKALSIHSDVEESGDSDDEGTADERHDDQGSLELEPTHGVKIPPNSEPLRKGNPISLSNSEYTVGNSSFQESTIQARTALPKVIVYPINVSPLQHDGLKVTAPSSTVTNVKPDIPYSDLIKDFDTNKYQKKQAEISEERPGTPLGTTIHAQLQRQQATDCSQEQGKGLLSPRSLGSTDSGYFSRSESADQTMSPPAPFLKGPPSTEKDLSKICGPRMSTTVVTAAQTTCPDKSFLSSGQMRPPLATKTLEERISKLISDNEAVVDDKQLDSVKPRRTSLSRRGSIDSPKSYIFKDSFQFDLKPMGRRTSSSSDIPKSPFTPTEKSKQVFLLSVPTLDCLPITRSNSMPTTSYSAIPTNVIPPPHPLRGSQSFDDKIASLYDDVFAPGPSPPQLQTGHTRTLVRQTAIEDSSSSEGHTFGPARSVDETYGSSVSNELLMARSKSFVQGSSLDKIKKSHQGRGIMFECETCRNRYRKLENFENHKKFYCSELHGPKTKAAIRDSEHKTTVNSTQPQVLHYRVATSTGVWEQTPQIRKRRKMKSVGDEDDEAPTNDTSSLSKNIAEVESQNKPGDAVSKQAALLDSYNTSVQRLAHDETETQITEQATEPKTLLHGKNHSVPIVHEKNELKRQGAGISVIQHTNSLSRPSSFEKADSFERISPISFPANKPEKFHSLNNAIKEEKHPRLSASQSLQAAEAAQHQIHHECRVSPNEKNAAVQPLRLVRQHNIQVPEILVTEEPDRDQECQCSDLEKTEKFNWPQRSETLSKLPTEKLPPKKKRIRLAEIEQSSADSSFESTLSRSFSRESSLSRASSFSASFDRDELAKNENASRADPVNKSVEFIKIPPCSNTLSVPGPHYREMRRAASEQINCTQPSMEVFDSRSKSFDYGSVSASKPASLKEISSSKLNASSGGPGHVPLLERRRGPLVRQISLNIAPEKNLPDSVSQASLQTDTSAESFQKLQSSETPLEDISSGSPHPQNYSKALQESVKSNNFLNFPSTQQSVGSSLNQPDQQSSLSRNSQASVKGSASQKNVNQHCKQEDCFAPKYQLHVKTLQVEQQYPSGLLEAAGMDQTGPSLEMFSKLNNNMSKPYVAQTAPNDNSSSVYQAEPFVVPVRIHSNIPSYGLASVAPIPQILVTQDQLSQPLCILNTASPTVAKDQASMPKTHKNNVRCLPCSQPISLFENLVYETEGKMPISLGSLNLIQKVPVGGLFPQQEATASSKRMLSPANSLDIAMEKHQKRVKDESGAVSITGAVSLHPLNIKPNELSKQKKPLLVRQSCTTEPLESFPLDPDDERGGANNSSDVLLSDSPESAQSKSYPFVKDSLEQDSLPASGTTDFAGRKPHQSVPQNTPSFLKILGDSQERMSPEFNESKKPDIHSQANSGTNFSAVNTGVTQRLSFPSLKTATNFTWCYLLKRKPVHLLQHDQQSSSYSSWSISPNNSNLLGLPTKIALSLLNSKQKDEKSSYTQAISTTFKSDALVYSSKWENNLLKQALVKQKAAKKFSNKENSELSTDQDIENIAKNEPRRVKIFDGGYKSNEEYVYVRGRGRGKYICEECGIRCKKPSMLKKHIRTHTDVRPYHCNYCNFSFKTKGNLTKHMKSKAHSKKCMDLGVSVGLIDDQDLEDYGEKQRLNYEQSGFDPEDSDGPEDEDNDNEDDDEDSQAESVLSATPSVSASPQHHPSRNASHEVSSADEESRVSDSFAEVHIDSADGLPKALLTKMTVLHTGHLDCRNSGPTSAAISREAKKESVQDRATSTEPAVSSETFHRSSCHQMYVDFPGAKEVLGSNTPSISTTATLKSTASTRLPSIPTDQSTQTTVVVPSVASPFAETQEQKHGEHQQTMKLGPPQTHLFSHLPLHSQQQTKSPYGVVPVGGIHVVPAGLATYSTFLPIPAGPVQLTIPAVSVIHRTTSALSDPAYAVSSTANHLGIAEVNSVVPCIPIGQINVPGIQSLSTPALQPLPSLSMETVNILGLAGTGLAPQIHHSGLTLNAVGLQVLTANPSSQRTSSPQAHIPGLQILNIALPTLASPVSPKTADDQGIVEAPASRNNTCETFQLQGSGSLATPDPAQIASAPSSQKALADKRYGMENRLEVGPARDYRRRDIPDNPDFESSDLESPKKPKCNISAVQVGQPSGSEPPAKVNPDILSSSPRHQMVLQDKEFHRPKRLPKLDLSDGSSDDEDRLIIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNF5</accession>
    <name>A0A6I9YNF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein DDI1 homolog 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106551662</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925280.1">
        <property type="nucleotide sequence ID" value="XM_014069805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNF5"/>
    <dbReference type="GeneID" id="106551662"/>
    <dbReference type="KEGG" id="tsr:106551662"/>
    <dbReference type="OrthoDB" id="1047367at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05479">
        <property type="entry name" value="RP_DDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000005">
        <property type="entry name" value="DNA damage inducible 1 homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057273">
        <property type="entry name" value="Ddi1/2_HDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397:SF3">
        <property type="entry name" value="DNA DAMAGE INDUCIBLE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397">
        <property type="entry name" value="SODIUM-GLUCOSE COTRANSPORTER REGULATORY PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24669">
        <property type="entry name" value="Ddi2_HDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ddi1/2 HDD" evidence="7">
        <location>
            <begin position="113"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartic peptidase DDI1-type" evidence="6">
        <location>
            <begin position="207"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="48"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013925280.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="41676" checksum="1DE3E09807D7D65A" modified="2020-10-07" version="1">MLVIGTTGSQTSFLPEGELPECAKLAYGPGRDDMRPEELADQELAEALQKSAEEAGMPRIDFSSIAVPGTSAQFPQSLQLPPPAPTPTPPAPSQAPAPTSGPPEPPSIPQGLDNPALLRAMLLANPHELSLLKERNPPLAEALLSGDLEKFTQVLVEQQQDRARREQERIRLFSADPFDLEAQAKIEEDIRQQNIEENMTIAMEEAPESFGQVVMLYINCKVNGHPVKAFVDSGAQMTIMSQACAERCNIMRLVDRRWAGIARGVGTQRIIGRVHLAQVQIEGDFLPCSFSILEEQPMDMLLGLDMLKRHQCSIDLKNNMLVIGTTGSQTSFLPEGELPECAKLAYGPGRDDMRPEELADQELAEALQKSAEEAETSDTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YR45</accession>
    <name>A0A6I9YR45_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Delta-sarcoglycan isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SGCD</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sarcoglycan beta/delta/gamma/zeta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927013.1">
        <property type="nucleotide sequence ID" value="XM_014071538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YR45"/>
    <dbReference type="GeneID" id="106553105"/>
    <dbReference type="KEGG" id="tsr:106553105"/>
    <dbReference type="CTD" id="6444"/>
    <dbReference type="OrthoDB" id="496749at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048738">
        <property type="term" value="P:cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006875">
        <property type="entry name" value="Sarcoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039972">
        <property type="entry name" value="Sarcoglycan_gamma/delta/zeta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12939:SF6">
        <property type="entry name" value="DELTA-SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12939">
        <property type="entry name" value="SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04790">
        <property type="entry name" value="Sarcoglycan_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013927013.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="31949" checksum="CB5A77E4EB74D815" modified="2020-10-07" version="1">MPQEQYAQRSTMQSSEGPHVYKVGIYGWRKRCLYFFVLLLMILILVNLAMTIWILKVMNFTIDGMGNLRITEKGLKLEGDSEFLKPLYAKEIRSRTGNPLYFQSAQNVTVNILNEESKVLTRLVTGPKAVEAYSQKFQVLTTSGKLLFSADDNEVVVGAERLKVLGAEGTVFPKSIETPNVRADPFKELRLESPTRSLVMEAPKGVLINAEAGNLEATCRTELKLQSKDGEIKLEASKIKLPRLPQGSYSPSGSNQKIYELCVCSNGKLFLSQAGSSSTCQINPSICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRP3</accession>
    <name>A0A6I9YRP3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C1orf167 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013926982.1">
        <property type="nucleotide sequence ID" value="XM_014071507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRP3"/>
    <dbReference type="GeneID" id="106553077"/>
    <dbReference type="KEGG" id="tsr:106553077"/>
    <dbReference type="CTD" id="109314266"/>
    <dbReference type="OrthoDB" id="9050378at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031473">
        <property type="entry name" value="DUF4684"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38493">
        <property type="entry name" value="CHROMOSOME 1 OPEN READING FRAME 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38493:SF1">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013926982.1"/>
        </source>
    </evidence>
    <sequence length="553" mass="63325" checksum="C40B8FF29F6237C3" modified="2020-10-07" version="1">MLSSTLLTCQEDNYSCLRSSSGTPPTTNKKDELKDQGYLRTIDREERCSGTFCGSELYLKNLLPDVFSPKSLESSLKTSSEEPSLSRRLSMDDTHEEMPPTLDPSGVPEVLLGHSLEKNAGDFAAPEVGDKHGEAGGDPITTSSVDEASLSTALQKVGLFEETIWRSPRKGFQPTNTRVWPRYLQKDGGPLRGYFVPLEIKKLGDAFKRWRSHWEKRQQIHLLALRIQTRLIRRCFEAWQSLAQRKRHSQAAMEHLWAQSLRASFRQWGLMAQARRNAKMAVIELLGAKHPTSCGPWRDPSRRNWPPKYIENTLDSLLCLRTLQNAFLTWEARCQEAQRAGAFHRGLTLRQLREVLGQWRQRTRGLNPLRLTEGCLLALPDWQEPSSSPALPCASQAVQPVEPSLISLTSLGTVEESSSLPAPTSRCPNCSFDRENPTCVTNRASPLPLNWQAASKYGRLWKRSVQLHRFQGVRDARQLAKAWLLWKDACRTELAIQTLRWWRGARWVWEMWRRRWLQLRVAQRFHEAEEAQLLKTAFGKWRRLAVVSRGQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRY5</accession>
    <name>A0A6I9YRY5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TP53-regulated inhibitor of apoptosis 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TRIAP1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate(in) = a 1,2-diacyl-sn-glycero-3-phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:36435"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIAP1/MDM35 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926921.1">
        <property type="nucleotide sequence ID" value="XM_014071446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRY5"/>
    <dbReference type="GeneID" id="106553027"/>
    <dbReference type="KEGG" id="tsr:106553027"/>
    <dbReference type="CTD" id="51499"/>
    <dbReference type="OrthoDB" id="19091at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007918">
        <property type="entry name" value="MDM35_apoptosis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403:SF1">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05254">
        <property type="entry name" value="UPF0203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013926921.1"/>
        </source>
    </evidence>
    <sequence length="103" mass="11619" checksum="86E766D87210D8FD" modified="2020-10-07" version="1">MRRFRRRCGSDAAPASGALRQSAMNSVGEACTELKRDYDQCFNRWFAEKFLKGEAGAEGDPCVQLFKRYQLCVQNAIKEKDIPIEGLEFMGSSKEKSENSSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS52</accession>
    <name>A0A6I9YS52_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc transporter 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SLC30A6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Has probably no intrinsic transporter activity but together with SLC30A5 forms a functional zinc ion:proton antiporter heterodimer, mediating zinc entry into the lumen of organelles along the secretory pathway. As part of that zinc ion:proton antiporter, contributes to zinc ion homeostasis within the early secretory pathway and regulates the activation and folding of enzymes like alkaline phosphatases and enzymes involved in phosphatidylinositol glycan anchor biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer with SLC30A5; form a functional zinc ion transmembrane transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927117.1">
        <property type="nucleotide sequence ID" value="XM_014071642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS52"/>
    <dbReference type="GeneID" id="106553191"/>
    <dbReference type="KEGG" id="tsr:106553191"/>
    <dbReference type="CTD" id="55676"/>
    <dbReference type="OrthoDB" id="5382797at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000009">
        <property type="entry name" value="zinc transporter 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052005">
        <property type="entry name" value="CDF_SLC30A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46531">
        <property type="entry name" value="ZINC TRANSPORTER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46531:SF1">
        <property type="entry name" value="ZINC TRANSPORTER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="14">
        <location>
            <begin position="35"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013927117.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="51029" checksum="590493848FF7855C" modified="2020-10-07" version="1">MGTIYLFRKSQRSLLGKLTQEFRTVTADRRSWKILLFGTINILCTAFLLMWCSSTNSIALTAYTYLTIFDLFSLITCLISYWVMMKKPSSSYSFGFERFEVLAVFASTVLAQLGALFILKESAERFVEQPEIHTGRLLVGTFVALTFNLFTMLSIKNKPFAYVSEAASTSWLQEHVADLTRSLCGIIPGLSSIFLPRMNPFILIDLAGALALCITYMLIEINNYFAVDTASAVAIALMTFGTMYPMSVYSGKVLLQTTPPHVIGQLDKLIREVSTLDGVLEVRNEHFWTLGFGSLAGSVHVRIRRDANEQLVLAHVTNRLSALVSILTVQIFKDDWIRPTLATGPIGNNMLNLSDHYIIPMPSLKAAENSDHVTSTPTKPSSPPPEFAFNTPGKNMNPIILMNTQTRPYGLNFNHGTVPYSSVFTQGFGLPGTGVTHGFRTGFINVPQRYGTGTRGQLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS61</accession>
    <name>A0A6I9YS61_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nucleolar protein 11-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106553415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927383.1">
        <property type="nucleotide sequence ID" value="XM_014071908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS61"/>
    <dbReference type="GeneID" id="106553415"/>
    <dbReference type="KEGG" id="tsr:106553415"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042859">
        <property type="entry name" value="NOL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048897">
        <property type="entry name" value="Nol11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012584">
        <property type="entry name" value="NOL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15633">
        <property type="entry name" value="NUCLEOLAR PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15633:SF2">
        <property type="entry name" value="NUCLEOLAR PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20998">
        <property type="entry name" value="Nol11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08168">
        <property type="entry name" value="NOL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Nucleolar protein 11 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolar protein 11 C-terminal" evidence="8">
        <location>
            <begin position="397"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013927383.1"/>
        </source>
    </evidence>
    <sequence length="714" mass="80225" checksum="53B2A9640D282B4F" modified="2020-10-07" version="1">MAAFREWFTLCSLPGTPPQAPLGLEPDEDRVLVTDRGRTVTLYKVSDQKPLGCWSVKQGQTISCPATWNYETEEFILVHDDKVLQIWKEDDTDLDKVFKATLSSDVYRIHSLPHTEPLVLFKGGAVKNLDALLADPKQEVENVISNEVIRWSNALIGVEKRVILFITEQDGNYFIYVQHCNPYTLQKFKFEQGVGSSTPLSFAAYLKNKVITLFCLYSNGCVYKILVPLQESKTAETQILPKSILLRLSVGGNILKGTSIVILDEDHIAVTGGLDSSESNVKDCFSIWNIKFQTLEASKDLPQGTTGQCWAYNDKLFIIHGRELEVILYKCKTSSLAAAVGKSKDTYVSEMKTLPVDWNRLQEDGIICAEPDKAAKDESKRLLRSRKNAQEGTLTVEQLLNIIKGASQNTVEEKLLAFLSSTPVTVFQASVGSIISALVNRCKAESSFFPRSCLVQLIQTQGLSYSMCPELMAIAVEKTDIHLLQLCLQQFPDIPEAVICTCLKIFLSIGDDHLETMNMNSDFVPDHVHNVQNNKMDELSKVVQNGCTFELIEEDSCDTQTIQKSQEIELSEICPVGPRKATLLNTIICSAYSETFILPHLKDLSAKEVILFLQYLQYVYVQCSKESSTHFPGILHLTIQQIMDWLCLLLDAHFTVVVMLPEARGLLSKLHKFVRAQVHFYSELNKIEGSLKHLQRINHPEDKGLYSIEVIKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTD8</accession>
    <name>A0A6I9YTD8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyrosyl-DNA phosphodiesterase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013927813.1">
        <property type="nucleotide sequence ID" value="XM_014072338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTD8"/>
    <dbReference type="GeneID" id="106553770"/>
    <dbReference type="KEGG" id="tsr:106553770"/>
    <dbReference type="OrthoDB" id="9975959at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927813.1"/>
        </source>
    </evidence>
    <sequence length="96" mass="10546" checksum="6F9902FF7CF9F03B" modified="2020-10-07" version="1">MEGGWADREMEQAASEPKERVDSAEEGGPREDLEEQRKQLCLDFASISNCGGAASAERYLAENGWDMQKALNSYFDPSPEQDVDRMSHPVPATGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVQ2</accession>
    <name>A0A6I9YVQ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA topoisomerase 2</fullName>
            <ecNumber evidence="15">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106554533</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14 15">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013928708.1">
        <property type="nucleotide sequence ID" value="XM_014073233.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554533"/>
    <dbReference type="KEGG" id="tsr:106554533"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.268.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF36">
        <property type="entry name" value="DNA TOPOISOMERASE 2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 14 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 14 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14 15" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="18">
        <location>
            <begin position="672"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1056"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1155"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1221"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1431"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1100"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1157"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1275"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1299"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1315"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1347"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1371"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="14">
        <location>
            <position position="762"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="1464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013928708.1"/>
        </source>
    </evidence>
    <sequence length="1464" mass="165418" checksum="90F81C2C942E6BC5" modified="2020-10-07" version="1">MAKSGGGGGGGGNGSGGAAGGGGSGGLTWVTLFDNQNNASKKEEPEHTNKNDSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVFDEDTGMNCRDVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSDPKIKHFEGDDYTCVTFQPDLSKFKMEKLDKDIVALMTRRAYDLAGACKGVKVMLNGKKLPVNGFRSYIDLYVKDKLDETGLALKVTHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQIVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQIQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLACLDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLYTCCKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKIPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFKGAIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLNYYGLRKEWLAGMLGAESTKLNNQARFILEKIQGKIAIGKNNTELKDIRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEEMQNLNDDNSSSSGSTSGPDFNYILNMSLWCLTKEKVEEIIKQRDSKGKELNELKKKSSSDLWKEDLAAFVEELEKVEAQEREDVLAGMTGKPIKGKVGKPKMKKLHLEETMPSPFGRRIVPQITSAMKADTSKKFLKKKKGDLDTSAIKLEFDEEFGGTPIEGAGEELLNTPGPVIKTPKPKREKKEPGTRVRRTPSATKTSAKKVKKRNPWSDDETKSESDLEENDPVIIPRDSLLRRAAAERPKYTFDFSEEEEDADDDEDANNNDLNELKVKTSPNINDREDEFVPSDSLEKDEYDFSPIKTKPSSEKVSQDKTNQDFGNLFTFPSYSRKANIDTSKFDSDEEDSTPVFSSTFIPKQTETLSSKTVVTKKAKLSSDMPSKLKRSPKPKKMELINSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVZ2</accession>
    <name>A0A6I9YVZ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Myelin expression factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MYEF2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928176.1">
        <property type="nucleotide sequence ID" value="XM_014072701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVZ2"/>
    <dbReference type="GeneID" id="106554100"/>
    <dbReference type="KEGG" id="tsr:106554100"/>
    <dbReference type="CTD" id="50804"/>
    <dbReference type="OrthoDB" id="610462at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12662">
        <property type="entry name" value="RRM3_MYEF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000034">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein M isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034631">
        <property type="entry name" value="MYEF2_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF15">
        <property type="entry name" value="MYELIN EXPRESSION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="240"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928176.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="33082" checksum="54A83CA133DEB0BE" modified="2020-10-07" version="1">MFNGQFLFDRPMHVKMDDKSIPHDDFRAADNKTPQLPRGLGGIGMGLGPGGQPISATQLSMGSGMGNVTPGGMGVDGPGFGGMNRMSGGVGGFGGMKGMANMGGFGGVNRLGGMGSVDDFRGNMGSGMSGGLGTGMAAGMGEMFRGGMGGTLDRDFGRSDIALSRGFGDTFGRMGSGLSGMNSMTGGMGMGMGMDRMSTSFDRMGPTMGSALERNLDMDRGFVPGPMGSGVRDRVGTKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMENGKSKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLDRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX00</accession>
    <name>A0A6I9YX00_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger CCCH domain-containing protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ZC3H13</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928135.1">
        <property type="nucleotide sequence ID" value="XM_014072660.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554060"/>
    <dbReference type="KEGG" id="tsr:106554060"/>
    <dbReference type="CTD" id="23091"/>
    <dbReference type="OrthoDB" id="6022762at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052824">
        <property type="entry name" value="m6A_RNA_Methylation_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13585">
        <property type="entry name" value="CHASCON, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13585:SF19">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="76"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="368"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="452"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="500"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="635"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="649"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="701"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="900"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="976"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="987"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1014"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1091"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1137"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1158"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1177"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1208"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1238"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1324"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1348"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1369"/>
            <end position="1387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928135.1"/>
        </source>
    </evidence>
    <sequence length="1604" mass="188742" checksum="1ABCAAAD78F766FF" modified="2020-10-07" version="1">MSKIRRKVTVENTKTISDSTSRRPSVFERLGPSTGSSAETQCRNWLKTGNCLYGNTCRFVHGPSPRGKGFSSSYRRSPERPTGDLRERMKNKRQDIESDSQKHNTEEQPSPVRKESTRGRHRGKEDIKITKERTPESEEDNPDWETNRDDSDNGDVNYDYDHELSLEMKRQKIQRELMKLEEENMDKREEIVIKKEISPEAVRSKLSSSPSPRKSSKSPKLRTSPKSSSTATKREKKVSTVSSPLLEPQRNSKNNHNKKKGPRTPSPPPPVQEDSTQGKKHKEKYKAKERVEEKPKEIKERGRDFERHNKEKKEKQRDPSESSHRQRRSPSPEDHSGSNSSSREYSPATRRRQTSWAPAKSSSHKHSFSPPRRSASPPTQHHSPVSSMHHSSSSQSGSSIQRRSPSPRHKRTPSPSYKRTTSPPVGRSSSPYPHRASPSPQQKQASSRHRSPVRERSRHDHERISQSHDRRHERRDETRGKRGRERDNRDERDYDQEQSTPRDHRDDRESRDGRDRRETRDNRERRDPRESRDARDSRDAREFSRDNKESRDQRDVRSTRDSHDYRERECREHKKEEQYLEESRSYARTHGREDNSRAESRTDSRSETRNESRISGRSRGRGPELTDKGNRGTRSSQVDSHNSSSSYHESWESRSNYPDRDRYESREHARDSSFERRHGERDKRDNRERDQRQSSPIRHQGRNDDIDRDERLEERRVDRNEDRRDERPREREREREREREAERDRARERERERERDKDRERERDRDHDRERERERERERERERERERERERERERGRERDKERERQREWDDKERGREERRDKREDVREERTTRDSREERKSKKRHRNESSPSPRKSPKRRREHSPDSDAYNSGEDQNEKRRLLSQVVRPQESRSLSPSHLAEEKQSRWKESDRKPERKESLRHEESDYKERGTSGDKQREQREGNESTRTRIQDNVSKRDSEEHEAVDHMHEDKKKAKIQKKIAKKKKEDDGGRLERYISEPPVEKTQVFSPKKGQKKKNAEKKRKRSKGDSEASEEDSGPHQKKKKGPRTPPVTTKEEVVEAVPEKTAVVEVAPKREDTTFSDWSDEDVPERGETVLERISEEPHRTSHRTRVETLEVSHVVEEAPHLRPAEQKRSSSLCSNRSRTSSRLRSPSNDSAHRSGDDQAAVKKILHSSSSDRDRRCLEIAGERKSRIDQLKRGVASRSTSSDRQDSRSHSSRRSSPESDRQGHSRSGSFDSRERVQDRDRHEHDRERERERRDGRQREWDRDAAKSEWLRSRERDRIRERDRQREKRRDLDREKERLLSENLERERGVSTSSQAEISKHSETKASKDHQAFEATALDSSSQEKERQDKDLSSLQGFEDGIEAEKVENVEGGDDETKIDDMQSMESGAGEYEPISDDELDEILAGDAEKRDDQQDEEKMPDPVDVIDVDWSSLMPKQPKEPREPGAALLKFTPAAVMLRVGISKQLAGPQLFTKIKETCQRSLEKPKDAESLFEHEFGALNMAALLRKEERAGLLCNLGPCCKALCFRRDSAIRKQLVKNEKGTTKQTYTNPPVVDNELLRLSLRLFKRKTVQQGSGPDKAEDTKLSQPYVPEMCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YX14</accession>
    <name>A0A6I9YX14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EIF4A1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_013928150.1">
        <property type="nucleotide sequence ID" value="XM_014072675.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106554078"/>
    <dbReference type="KEGG" id="tsr:106554078"/>
    <dbReference type="CTD" id="1973"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="7"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="158"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013928150.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="28153" checksum="2987D7F3D97BB978" modified="2020-10-07" version="1">MNSSSSSSFTFSGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQVGSNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKALVWVIKRLRGNKHQGSVSQPWQLEXDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXM7</accession>
    <name>A0A6I9YXM7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8 9">Coiled-coil domain-containing protein 126</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8 9 10" type="primary">CCDC126</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8 9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928699.1">
        <property type="nucleotide sequence ID" value="XM_014073224.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928700.1">
        <property type="nucleotide sequence ID" value="XM_014073225.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928701.1">
        <property type="nucleotide sequence ID" value="XM_014073226.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013928702.1">
        <property type="nucleotide sequence ID" value="XM_014073227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXM7"/>
    <dbReference type="GeneID" id="106554529"/>
    <dbReference type="KEGG" id="tsr:106554529"/>
    <dbReference type="CTD" id="90693"/>
    <dbReference type="OrthoDB" id="9946758at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042759">
        <property type="entry name" value="CCDC126"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027833">
        <property type="entry name" value="MGT5A-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46941">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46941:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15027">
        <property type="entry name" value="MGT5A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="MGT5A-like N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013928699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013928700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013928701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013928702.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="15574" checksum="85C0C9A27B68168E" modified="2020-10-07" version="1">MFFAFSRKNVSQKLSLLLLVFGFIWGIMLLRYTFQHPRQQSSAELREQILDLSKRYVRALAEENKNVINGDNGVAMAGYADLKRTIAVLLDDILQRLVKLENKVDYMVVNGSTTNTTNGNLMPSTTIKRVNAAHNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXU4</accession>
    <name>A0A6I9YXU4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protocadherin gamma-B5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106554311</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928435.1">
        <property type="nucleotide sequence ID" value="XM_014072960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXU4"/>
    <dbReference type="GeneID" id="106554311"/>
    <dbReference type="KEGG" id="tsr:106554311"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF73">
        <property type="entry name" value="PROTOCADHERIN GAMMA-B3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="79"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013928435.1"/>
        </source>
    </evidence>
    <sequence length="310" mass="34574" checksum="40CD2F0AF10247A0" modified="2020-10-07" version="1">PLPYYLSINSESGTLYSQRSFDYEQLREFEVQVKAEDGGSPPLSSNATLRLFIQDQNDQNPQILYPSPGAEGSASFEMVPRSAEMGYLVTKVVAVDSDSGHNAWLSYHLLQATEPGLFTIGAHTGEIRTLRTFLERDALKQKLVVLVKDNGQPPLSATVSLNLIFAENFQEALPEINNQTMDSEQQSELQFYLVLALTLISFLFLLTVTLVIMMKLRQSGNLTFLPCFAPIPHSSNAIIFPPNYEEGTIPYSYQLCLSSESKIHEITFPAPKVQPTEYISCNQNSDVLLPTNVLNSEMEKIDLQAQPNTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYD4</accession>
    <name>A0A6I9YYD4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">UBX domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UBXN7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ubiquitin-binding adapter that links a subset of NEDD8-associated cullin ring ligases (CRLs) to the segregase VCP/p97, to regulate turnover of their ubiquitination substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with neddylated CUL2, ubiquitinated HIF1A, and VCP/p97.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929059.1">
        <property type="nucleotide sequence ID" value="XM_014073584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYD4"/>
    <dbReference type="GeneID" id="106554816"/>
    <dbReference type="KEGG" id="tsr:106554816"/>
    <dbReference type="CTD" id="26043"/>
    <dbReference type="OrthoDB" id="270602at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14345">
        <property type="entry name" value="UBA_UBXD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01773">
        <property type="entry name" value="UBX_UBXN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000097">
        <property type="entry name" value="UBX domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000079">
        <property type="entry name" value="UBX domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054109">
        <property type="entry name" value="UBA_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017346">
        <property type="entry name" value="UBX_7/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22566">
        <property type="entry name" value="UBA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037991">
        <property type="entry name" value="UCP037991_UBX7/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="UBX" evidence="12">
        <location>
            <begin position="427"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="318"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013929059.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="57593" checksum="80A1E374931616B6" modified="2020-10-07" version="1">MLVPSLEISAYFILKLRETKKDRRPPFLGVCNVSVKSEKIGISGGASESAGKHMLEACNNNLEMAVTMFLDGGGIAEEPSTSSASVSNLRPHTEDEVRAPIPQKQEILVEPEPLFGAPKRRRPARSIFDGFRDFQTETIRQEQELRNGGAIDKKLTTLADLFRPPIDLMHKGSFDTAKECGQMQNKWLMINIQNVQDFACQCLNRDVWSNDAVKNIIREHFIFWQVYHDSEEGQRYIQFYKLGDFPYVSILDPRTGQKLVEWHQLDVTSFLDQVTSFLGEHGQLDGHSTSPPQKRTRSESLIDASEDSQLEAAIRASLQETHFDSEQIKQDSRSDEESESELFSGSEEFISVCGSDDDEPESSAKSRKSPHKDLGCRKEEGRKPQPEPTSRTEPEMIINHRGLSSVDSGVLEETETKLDDLQKELSVNGPKAQLMLRYPDGKREQISLPEQAKLLALIRHVQSKGYPNERFELLTNFPRRKLSHLDYNITLQEAGLCPQETVFVQERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYS6</accession>
    <name>A0A6I9YYS6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein-associating with the carboxyl-terminal domain of ezrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SCYL3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929467.1">
        <property type="nucleotide sequence ID" value="XM_014073992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYS6"/>
    <dbReference type="GeneID" id="106555208"/>
    <dbReference type="KEGG" id="tsr:106555208"/>
    <dbReference type="CTD" id="57147"/>
    <dbReference type="OrthoDB" id="9942861at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF15">
        <property type="entry name" value="PROTEIN-ASSOCIATING WITH THE CARBOXYL-TERMINAL DOMAIN OF EZRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="593"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="599"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929467.1"/>
        </source>
    </evidence>
    <sequence length="735" mass="82145" checksum="91FB569C374957BD" modified="2020-10-07" version="1">MGSENSALKRDAVDEPSFTLPSGVNIYSAILQHDKLASVFVYNRENEDNVNRAAKHLKTLRHPCLLRFLSCTVELDGIHLVTEHVQPLEKVVEALSTSEICAGIYDVLQALVFLHDRGNLTHNNICLSSVFVSEDGHWKLGGMETVCKQNEATAEFLNSIQSIREKTGIPPEEQSVDFQILPEGHGHSRDAYSFGVMVEKLLSFLNEEGPVCTSHNVVKYCLLKPERELTPALESLASHNFFRNDFLEVVSFLNSLTLKSEEEKTEFFKFLLDRVAGLSEKLIALRLVPLLLNQLVFAEPVAVKSFLPHLLGPKKDKLSESQINGLLSPAMFQAHVSPVLLKLFEVHEEHVRMVLLSHLDAYAELFTQKELKNIILPQVLLGLRDTCDSLVAVTLQSLGVLVSFLGPDVVVGGDRTKIFKRSAPSFTKIMDLSSEDSSDDVVSCEGKLIYLPLQHNSSMFLKCHSSGSLSFSNKNNVPGEGFHIALRKGTQDTLNLYNIPSGISNINNGNNCHHVTEKTEEWPDWNECEELKPERNRIADTKLKDFCVNSHPCLANHDGEEKSPFKLSSGNTHYDRGINDIAELQLPPNTFAPKKELGTLQNNPLPESISNNDGCDPRDQDQKSYLTKTSFLEKSPKLKSGLGEEFVIQVKRKELHDTELDWFADMVPDIKSSLLVLPELETQSFTQSNSETVLSSVSDGQEMQFSSKFAASEIAEVDGVGWEEDDLNWEEDTNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZF6</accession>
    <name>A0A6I9YZF6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein LOC106555733</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106555733</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930103.1">
        <property type="nucleotide sequence ID" value="XM_014074628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZF6"/>
    <dbReference type="GeneID" id="106555733"/>
    <dbReference type="KEGG" id="tsr:106555733"/>
    <dbReference type="OrthoDB" id="9028103at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="677"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013930103.1"/>
        </source>
    </evidence>
    <sequence length="799" mass="88673" checksum="DAF6537A798C1836" modified="2020-10-07" version="1">MYLSRKDNAGCSQRSRKNIGALNQELPIFSPAKQYLSSGVPKADTNLAYGRSTPARRQDTMDLVTGMDVGAEIEVTEDGDLDELPLHLENVQNCLCLESVDISSSDDDGESHLTFFLPVRRPPRPPQRRSIYRPAAAAKLSASEKWVPPLFSCPLRPGGPPPPGIPAFGAYQCQKCLQVFMEEWHYTQHLQDHAQEEEELAQRHAAQNPARPPSPPRKLRCLECGKRFLRPEQFARHTKWHLKLVRLGIKIHHRKVSRRSSQGSYVYKPLGGTRNPLEGKGVSDLPVVQEILNQPASLQVPKPQRTGKQKAAKHQKRRSAETWEPQELLSATQAGNSMAVLDGATGINLLQPWQKQEGVLEGQVAGGLFQPAVMSAENQIIILDGDEAEAVPVAAERHYAELQATIFDGQGTSNTVQPVFLRTEEQAALLDPSVSTHPLQAGGEKDQEAVGPEWVGQNPCTVYRTVLLQADDQAAVVDGQTEPGPFQQVIIKTSEVSPTLYLDPQMSSLDPGAYHLVPEPQYVTFPYGNSLPLDHSEATEEGEKAWESQEASFCLPNLYQASKQQQPLPAVTTSPRSPVVMRLLPCPAGDHPEVLDLEYDTGGGIPVASEPWDVNMHTGQEAPSPTQAAEDGFVVVEMQSESDQQGLAVGEHLDRPQKRTFRRARKQPLRNGSIWRFKCPDCGVCYSQHSQLRSHQKGRKRRGRSFLCECGSPFRGLLHLLRHQLRHLEEALFICSTCGKSLRGHAGLQRHDSCQPGLARFSCPCGVNFRRLSRYLWHHVRSQRPGLRVYTLVGFLSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0C2</accession>
    <name>A0A6I9Z0C2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Engulfment and cell motility protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106555655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930022.1">
        <property type="nucleotide sequence ID" value="XM_014074547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0C2"/>
    <dbReference type="GeneID" id="106555655"/>
    <dbReference type="KEGG" id="tsr:106555655"/>
    <dbReference type="OrthoDB" id="28413at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13359">
        <property type="entry name" value="PH_ELMO1_CED-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000053">
        <property type="entry name" value="Engulfment and cell motility protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF8">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELMO" evidence="2">
        <location>
            <begin position="58"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930022.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="45827" checksum="1F6B5558151CD2AC" modified="2020-10-07" version="1">MADASAQKQLRSAILTHVIRGNRPIKTEMAHQLYVLQVLTLNLREERMMTKMDPSDQAQRDALFELRRIAFEVEAESGGAEKRKAIYSRDYKTLGFTNPVNPALDFLQTPPGMLALDNMLYLAKHHQDAYTRIILENSSPEDKHACPFGRSAIELTKMLCEILQIGELPNEGRNDYHPMFFTHDQALEELFVIGIQLLNRTWKEMRATAEDFHKVMQVVREQITRALPAKPPSLDQFKGRLRNLAYSEVLRLRQSERMSQDDIQSPPIVELREKIQPEILELIKQQRLNRLCEGSSFRKVGNRRRQERFWYCRLALNHKTLHYGDLEENAQGGATLESLQEKIPVADIKAILTGKECPHMKEKGALKQNKEALELAFSILYDPDEALNFIAPNKYEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0E1</accession>
    <name>A0A6I9Z0E1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Wilms tumor protein 1-interacting protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106555081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013929325.1">
        <property type="nucleotide sequence ID" value="XM_014073850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0E1"/>
    <dbReference type="GeneID" id="106555081"/>
    <dbReference type="KEGG" id="tsr:106555081"/>
    <dbReference type="OrthoDB" id="25414at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035331">
        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047172">
        <property type="entry name" value="Ajuba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24219">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN JUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24219:SF6">
        <property type="entry name" value="WILMS TUMOR PROTEIN 1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="73"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013929325.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="13347" checksum="2570B441CD4FA79A" modified="2020-10-07" version="1">MGAYPELSAPSPRASLLPEEAPPADAEPAKLRLPCQVTPARESGPSQVERRLEALTLELEKELELHVRKEYFGICIKCGKGVYGASQACQAMGNLYHTNCFTCCSCGPQLTCFLSRNSLSSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0T1</accession>
    <name>A0A6I9Z0T1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Unconventional myosin-Ie isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MYO1E</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930187.1">
        <property type="nucleotide sequence ID" value="XM_014074712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0T1"/>
    <dbReference type="GeneID" id="106555800"/>
    <dbReference type="KEGG" id="tsr:106555800"/>
    <dbReference type="CTD" id="4643"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032835">
        <property type="term" value="P:glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11827">
        <property type="entry name" value="SH3_MyoIe_If_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000010">
        <property type="entry name" value="Unconventional myosin-Ie"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035507">
        <property type="entry name" value="Ie/If_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF341">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 11" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="14">
        <location>
            <begin position="19"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="15">
        <location>
            <begin position="730"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="1057"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="11">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="918"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="973"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="997"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1029"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1039"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013930187.1"/>
        </source>
    </evidence>
    <sequence length="1114" mass="126809" checksum="08FCD1176DF65371" modified="2020-10-07" version="1">MGSKGHYRYHWQSHNVKHSGVDDMVLLSKISEDSIVENLKKRYLDDFIFTYIGSVLISVNPFKQMPYFGEREIEMYQGAAQYENPPHIYALADNMYRNMIIDRENQCVIISGESGAGKTVGAKYIMSYISRISGGGPKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSPGGEPDGGKISNFLLEKSRVVMRNPGERSFHIFYQLIEGASHEQKSSLGITHMDYYHYLSLSGSYKVEDINDKSDFQETLHAMKVIGISAQEQALVIQIVAGVLHLGNLSFKEVGNYAAVESEEFLAFPAFLLGIDQGRLKEKLTSRQMDSKWGGKSESIHVTLNVEQACYTRDALAKALHARLFDFLVDSINKAMEKDSQEYNIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIDYFNNKIVCDLIENKVNPPGIMSILDDVCATMHAVGEGADQTLLQKLQMQIGTHEHFNSWNQGFIIHHYAGKVSYDMDGFCERNRDVLFMDLIELMQSSELPFIKALFPETPQAEKKGRPTTAGSKIKKQANDLVGTLMKCTPHYIRCIKPNETKKPRDWEESRVKHQVEYLGLRENIRVRRAGYAYRRAFQKFLQRYAILTPETWPLWKGDERQGVLHLLRSVNVDSDQYQLGRTKIFIKAPESLFLLEEMRERKYDGYARAIQKAWRKHAARKKYVQMREEASDLLLNKKERRRNSINRNFVGDYIGMDDHPELRQFVGKREKIDFADTVTKYDRRFKGIKRDLLLSPKCLYLIGREKVKQGAEKGLVKEVLKRRIEVERILSVSLSTLQDDLFVLHEQEYDSLLESVFKTEFLSLLSKRYEEKTQRRLPLKFSNTLEVKLKKETWGPWKAGGSRQVQFSQGQGDAAVLRPSNKVLQVSIGPGLPKNARPTKRGASQPRGLPSKTHGQNYPLRAAPPPPGKLSEGLPNGVIKLQQHPRQPSGPAGQRASQKSLYTSMACPPLPRVSGAPGRASQQPSSLDFLKVPEQGAAGACRQTTSRPPPAGGRPKPRPQPQPKPQVPQCKALYAYDAQDTDELSFNANDIIDLLREDPSGWWTGRLRGKQGLFPNNYVSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0Z5</accession>
    <name>A0A6I9Z0Z5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synaptogyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SYNGR2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synaptogyrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929969.1">
        <property type="nucleotide sequence ID" value="XM_014074494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0Z5"/>
    <dbReference type="GeneID" id="106555613"/>
    <dbReference type="KEGG" id="tsr:106555613"/>
    <dbReference type="CTD" id="9144"/>
    <dbReference type="OrthoDB" id="10041611at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016579">
        <property type="entry name" value="Synaptogyrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838:SF19">
        <property type="entry name" value="SYNAPTOGYRIN-2 LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011282">
        <property type="entry name" value="Synaptogyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="22"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="189"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR011282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013929969.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25064" checksum="492C98DB66F755E0" modified="2020-10-07" version="1">MESGGGAYGAAKAGGTFDLWQFLQQPQVIARIFSAVFALIVFSCIIGEGYTNSDKTSELFCIFNHSEDACRYGIGIGVVAFLACVFFFMVDIYFPQISSATDRKYLVMADLAFSAVWTFLWFIGFCVLTNQWSSTQAKDVRIGADSARASIAFSFFSIFSWGLLIAFALQRYRMGVQDFDQSYIDPAPGPTPPYSSYPNASHESYQQPPFTHTGENSEGYQPPPVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z164</accession>
    <name>A0A6I9Z164_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription initiation factor TFIID subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TAF4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930327.1">
        <property type="nucleotide sequence ID" value="XM_014074852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z164"/>
    <dbReference type="GeneID" id="106555916"/>
    <dbReference type="KEGG" id="tsr:106555916"/>
    <dbReference type="CTD" id="6874"/>
    <dbReference type="OrthoDB" id="21060at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08045">
        <property type="entry name" value="HFD_TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000015">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1110:FF:000002">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1110">
        <property type="entry name" value="TAFH/NHR1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045144">
        <property type="entry name" value="TAF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037249">
        <property type="entry name" value="TAFH/NHR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003894">
        <property type="entry name" value="TAFH_NHR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138:SF18">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07531">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00549">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158553">
        <property type="entry name" value="TAFH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51119">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFH" evidence="8">
        <location>
            <begin position="508"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="884"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="926"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="890"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013930327.1"/>
        </source>
    </evidence>
    <sequence length="1001" mass="104887" checksum="D083D40EAFEE6241" modified="2020-10-07" version="1">MGLSGPEITKPGAGGVQGSVINHNARSQGSALDGTTTTSSTTTTNVQGGSEAAITPGTLNQGKSVVISTMATALSTRNGKIGTTAVQTLNGSNVVMNSHASFSATPVTANPTVVPAVAPLVNNGPGNIGKVNTVNAVLPSASNTVIQTSFLGPGVSSASSPSPTVISSQQPPNIGTGGPTVALVRPPIHAAGPLAAATQNGSSTVINSTINVGGFPTAAVAAATVGSGVSLQTSLVNSQPGSGVSAAAPTTQIIKAESPKTIVQTVTQQQTLAAAGQPSTTGGNMIIGQTMQAGLPNVAPNPGGVPPGPPGTPTGLAKGAANTVAQSLPRTATATTSGIRATLTPTVLAPRLPQPSQNPTNIQNFQLPPGMVIVRSESGQLLMIPQHALAQMQAQAHAQSQSQTTMTPRPATSTSASPVQLSTVQAPGTPIIARQVAPTTIIKQVSQAQTTVQPTTTLQRPPVVQPQIVLGSPAQTTALGTATTVQTGTPQRTVQGATATSTAATETMENVKKCKNFLSTLIKLASSGKQSTETAANVKELVQNLLDGKIEAEDFTSRLYRELNSSPQPYLVPFLKRSLPALRHLTPDSAAFIQQSQNQQPLTQPTTALTAVMLSSSVQRTAGKTTATVTSTLQQPVISLTQPTQVGVGKQGQPTQVVIQQSQKQGTLIRPPQVTLTQTPMVALRPPHNRIMLTAPQQIQLNALQTVPVVKQTVLPGTKALSTLSTQAAIAQKNKLKEPGGGSFRDDDDINDVASMAGVNLSEESARILATNSELVGTLTRSCKDETFLLPTPLQRRILEIGKKHGITEIHPDVASYVSHATQQRLQNLVEKVSETAQQRNISYKDDDRYEQASDVRAQLKFFEQLDQIEKQRKDEQEREILMRAAKSRSRQEDPEQLRLKQKAKEMQQQELAQMRQRDANLTALAAIGPRRKRKLDSPGSGSGTEGSSPSVAVPGCSGIGTTRQFTRQRITRVNLRDLIFCLENERETSHSLLLYKAFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1B1</accession>
    <name>A0A6I9Z1B1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Nuclear receptor subfamily 1 group D member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Rev-erbA-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">V-erbA-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">NR1D1</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="32">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="27">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929625.1">
        <property type="nucleotide sequence ID" value="XM_014074150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1B1"/>
    <dbReference type="GeneID" id="106555321"/>
    <dbReference type="KEGG" id="tsr:106555321"/>
    <dbReference type="CTD" id="9572"/>
    <dbReference type="OrthoDB" id="7634782at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044321">
        <property type="term" value="P:response to leptin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07166">
        <property type="entry name" value="NR_DBD_REV_ERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000013">
        <property type="entry name" value="Nuclear receptor subfamily 1 group D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF113">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 1 GROUP D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0010">Activator</keyword>
    <keyword evidence="17" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="23" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="18 27" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="8 27" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22 27" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21 27 32" id="KW-0675">Receptor</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="20 27" id="KW-0804">Transcription</keyword>
    <keyword evidence="15 27" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11 27" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 27" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="29">
        <location>
            <begin position="137"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="30">
        <location>
            <begin position="301"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="244"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00039258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00042197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00043097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_013929625.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="65187" checksum="0D5B43DB38C2B39C" modified="2020-10-07" version="1">MNDNYQLNRMATLDDNNNAPGGVISYIGSNGSSPSRTSPISLCGSDSLNGSCQLFSHPTFPNYFPPSPAGSLVQDSGRLYGSNSAGLSDDASPSSSSSSSYSSGGSPVGLQVTLDDGRRVSPIKSTSSSITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKKCLKNETCSIVRINRNRCQQCRFKKCLRVGMSRDAVRFGRIPKREKQRMLAEMQSAMNNMANNQLSAQCLPENLPTGHPQPSTLLLGHQSQPAPSSPPSRAASPPQISPCYSQQLTPPRSPSPGDVMEDVIAQVAKAHKEIFVYAHDKLPTGPQPPTGSNSSPSWDNNWLINGTNGLHLQNDNWNTGSASRPACHQNIHGHRFCPSGETDLSLKQDETRVLACPVNCHPHGYSGRTFQEIWEDFSLSFVSAVREVVEFAKHIPGFQELSQHDQVSLLKAGTFEVLMVRFASLFNVKEQTVTFMSRTKYNLEELWGMGMDDLLTSMFEFSEKLSALQLSEEELGLFTAVVLISADRSGIENPASVERLQETLIRALRALILKNHPQETSRFTKLLLKLPDLRTLNMHSEKLLSFRIDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1B5</accession>
    <name>A0A6I9Z1B5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C-reactive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106555720</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the pentraxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930089.1">
        <property type="nucleotide sequence ID" value="XM_014074614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1B5"/>
    <dbReference type="GeneID" id="106555720"/>
    <dbReference type="KEGG" id="tsr:106555720"/>
    <dbReference type="OrthoDB" id="547680at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001849">
        <property type="term" value="F:complement component C1q complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000070">
        <property type="entry name" value="Serum amyloid P-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051005">
        <property type="entry name" value="Pentraxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869:SF7">
        <property type="entry name" value="C-REACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869">
        <property type="entry name" value="C-REACTIVE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0011">Acute phase</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pentraxin (PTX)" evidence="12">
        <location>
            <begin position="102"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013930089.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="35445" checksum="FFBE43663C818044" modified="2020-10-07" version="1">MTLRGLERTSQMQILCRGKVPGSNFRRILLFVLRSREISEECPRGSPFGEVTWFCTQYRPKRASQSSCGQLVPPSSTKMVLLLSFLLILACLSGSFGHEDLRNKVFAFPAPSATSAVVLPISNQQPLTKLTVCLSYYTLLTRPYGLFSYATRSVDNDFLIYKSQPNEYAIHVGGGVVNFKVAQKEKPSWEHICVSWDSSNGLVQFWLNGVPLPRQGLKKGYSINPEGSVLLGQDQDNFGGGFDINQSFVGEISDVNLWPRMLSPVEVGLSDKNFMLPSPLINWRSLSYTIKNEVGKGDGVTRRPRLGLYDSRKCEEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1J1</accession>
    <name>A0A6I9Z1J1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 883</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZNF883</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930893.1">
        <property type="nucleotide sequence ID" value="XM_014075418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1J1"/>
    <dbReference type="GeneID" id="106556422"/>
    <dbReference type="KEGG" id="tsr:106556422"/>
    <dbReference type="CTD" id="169834"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF151">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013930893.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="44501" checksum="B2CF6A3839830187" modified="2020-10-07" version="1">MGTTESDPCESGSEWEPYGVLSEETEDEEDAVSHFGNLQEPGKSKGSQPEEWLNEDSSQGSDYTALLAQQKAHKGKRKNQCKVCGKSFTRKASLKVHQRIHTGEKPYKCTVCTKGFCDKTSFLRHQRIHTGEKPYKCPECGKSFNRTTNLITHQKTHVESREKTFHCSSCSKSFYNKYSLKTHWRSHTGEKPYKCSSCSKSFCDKSNLEAHWRVHTGERPFECSECGKSFSDRRTLLRHQRIHTGEKPYRCTECGKSFNDLATLLRHQKIHTGEKPYHCTECGKSFNQSSHLIRHQNTHDAKRHKTLEPKRGAKASVGTNIFLDIGSFAQGSYYQYAHPAEPFHWPLSTYSSSAEPGEAGMPNLWTIQETSVGDQAILVMKEFTEERTLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1X7</accession>
    <name>A0A6I9Z1X7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PEX14</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxin-14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931053.1">
        <property type="nucleotide sequence ID" value="XM_014075578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1X7"/>
    <dbReference type="GeneID" id="106556592"/>
    <dbReference type="KEGG" id="tsr:106556592"/>
    <dbReference type="CTD" id="5195"/>
    <dbReference type="OrthoDB" id="441517at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="128"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="196"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013931053.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29173" checksum="1993908172D15C93" modified="2020-10-07" version="1">MTGIAIGFHQLYKKYLLPLIVGGKEDRKQLQRIDSNISEMSGSVTQTVTQLQTTLASVQELLIQQQQKIQELTQELATSKATTSTNWILESQSISELKSEIYSLKGLLLNRRQFPPSPSAPKILSWQMPVKPASPSNPVTNNHNTSSDISPVSNESNSPSPVKENHSPEGTKVGCHLLSPAEEGEAMIDLTGQVRMEVQGEEEKREEQARIEEEEEEEEEDNRPDEEDVQTEDRRGGDGQINEQVEKLRRPEGASNENEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2G9</accession>
    <name>A0A6I9Z2G9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RING finger protein 223</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RNF223</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930424.1">
        <property type="nucleotide sequence ID" value="XM_014074949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2G9"/>
    <dbReference type="GeneID" id="106556001"/>
    <dbReference type="KEGG" id="tsr:106556001"/>
    <dbReference type="CTD" id="401934"/>
    <dbReference type="OrthoDB" id="252722at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16556">
        <property type="entry name" value="RING-HC_RNF183-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051435">
        <property type="entry name" value="RING_finger_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791:SF4">
        <property type="entry name" value="RING FINGER PROTEIN 223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="127"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013930424.1"/>
        </source>
    </evidence>
    <sequence length="326" mass="36432" checksum="01A2DA8C7493D7C4" modified="2020-10-07" version="1">MKEMTISQEKFSSVQQQGRKCFDEGALRIDTERIVEELKMALGSVMSCLTQIWHTEVPESPPSSPQSPTPADPRERHIPLNPIVIMSPSVENRPAVCSPVGSPLSSPIDGKTRLGSPTERPTSPIECSICFNTYDNLFKTPKVLQCHHTFCLECLARLTAALPPNRVEDQLPCPFCRQLTEIPLEGAPGLQTCKELMATLPPELQKEKSLWMEGTKLCCRQCSDPEHAHTYVTIDIAPSKPEVPETPTEGFIGRLSRCSICDDWKRVLLLSVLIIILFCIILWPVQCIVKTGNLRCLNKFTYPRPNYVPYHLTTPAPTLDLTKLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2I3</accession>
    <name>A0A6I9Z2I3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDAN1-interacting nuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106555695</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930439.1">
        <property type="nucleotide sequence ID" value="XM_014074964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2I3"/>
    <dbReference type="GeneID" id="106555695"/>
    <dbReference type="KEGG" id="tsr:106555695"/>
    <dbReference type="CTD" id="84529"/>
    <dbReference type="OrthoDB" id="1272at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029404">
        <property type="entry name" value="CDIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661:SF1">
        <property type="entry name" value="CDAN1-INTERACTING NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661">
        <property type="entry name" value="SIMILAR TO CDNA SEQUENCE BC052040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14811">
        <property type="entry name" value="TPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013930439.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="31814" checksum="D425E68BAAA51C89" modified="2020-10-07" version="1">MKLTKAQYEEVVQFLAQVPPTRQTLRKLKEKFPSQSQSTLLSIFSQEYQKLIKRTHAKHHTPEATDTYYQRYLKGVSKNAASPVLLELANEVDFAPSLMARIVLERFLQEQDGSVPSKTLINSLLRDPSQIPDGVLANQVYQCIVNDYCYGPLVDCIKHAIGYEHEVLLQEMLLKRNISFLAEDQLRAKGYDKTPDFILEVPIAVEGHIIHWIESKASFGDECSHQAYLNDQFWSYWNRFGPGLVIYWYGFIEELDCHRNRGILLKDCFPTDIAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z386</accession>
    <name>A0A6I9Z386_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ryanodine receptor 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106555911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930320.1">
        <property type="nucleotide sequence ID" value="XM_014074845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z386"/>
    <dbReference type="GeneID" id="106555911"/>
    <dbReference type="KEGG" id="tsr:106555911"/>
    <dbReference type="OrthoDB" id="8892755at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005790">
        <property type="term" value="C:smooth endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005219">
        <property type="term" value="F:ryanodine-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014808">
        <property type="term" value="P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006941">
        <property type="term" value="P:striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399">
        <property type="entry name" value="B30.2/SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399:SF7">
        <property type="entry name" value="RYANODINE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013930320.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17669" checksum="8C253CC987ACA8BE" modified="2020-10-07" version="1">MVGVFIQEKEMEKQKLLYQQARLHDRGAAEMVLQTISASKGEMGPMVAATLKLGIAILNGGNSTVQQKMLDYLKDKKDVGFFQSLAGLMQSCSVLDLNAFERQNKAEGLGMVTEEGSVITHERGNYYTSIDFLFSCCKLRVSTVQLLNTMQHAVRFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4W4</accession>
    <name>A0A6I9Z4W4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="9">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">EIF5A2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013931697.1">
        <property type="nucleotide sequence ID" value="XM_014076222.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013931698.1">
        <property type="nucleotide sequence ID" value="XM_014076223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4W4"/>
    <dbReference type="GeneID" id="106557096"/>
    <dbReference type="KEGG" id="tsr:106557096"/>
    <dbReference type="CTD" id="56648"/>
    <dbReference type="OrthoDB" id="9975114at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0385">Hypusine</keyword>
    <keyword evidence="12 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="10">
        <location>
            <begin position="83"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013931697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013931698.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="16693" checksum="0302F792036BA19D" modified="2020-10-07" version="1">MADEVDFTTGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDVFTGKKYEDICPSTHNMDVPNIKRNDYQLICIQDGYLSLLTESGEVREDLKVPEGELGKEIEGKYNAGEDFQVSVMSAMSEECAVAIKPCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7A0</accession>
    <name>A0A6I9Z7A0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline O-acetyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CHAT</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>choline + acetyl-CoA = acetylcholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:18821"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15355"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.6" evidence="6"/>
    <dbReference type="RefSeq" id="XP_013931700.1">
        <property type="nucleotide sequence ID" value="XM_014076225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7A0"/>
    <dbReference type="GeneID" id="106557099"/>
    <dbReference type="KEGG" id="tsr:106557099"/>
    <dbReference type="CTD" id="1103"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004102">
        <property type="term" value="F:choline O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008292">
        <property type="term" value="P:acetylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000001">
        <property type="entry name" value="Carnitine O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000004">
        <property type="entry name" value="Choline O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF14">
        <property type="entry name" value="CHOLINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="618"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013931700.1"/>
        </source>
    </evidence>
    <sequence length="637" mass="71961" checksum="6DA13C44CF274BB9" modified="2020-10-07" version="1">MPIREKITAKKENGAQSQDEMVLPKLPVPSLQQTLDRYLQCMKHLVTDEQYKKTKTLVEKFGAPGGQGEFLQKKLLERHEQMENWVLQYWLDDMYLNNRSALPINSSPGIIFACQQFQEADDQLRFAANLISGVLDYKALLDSHALPVDFARGQLSGHPLCMKQYYGLFSSYRLPGLTKDTLVSQKSNIMPEPDHIIVACNNQFFVLDVVINFRRLSEGDLFTQLRKIVKMSESEEERLPPIGLLTSDGRLEWATARAILMEDSTNRDSLDMIERSLCLVCLDSPAGEELNDTNRALQLLHGGGYSRNGANRWYDKSTQFVVGRDGVCGTVCEHSPFDGIILVQCIEHLLKHMKEGAKKLVRADSVSELPAPRRLRWKCSPEIQSHLASSAEKLERLVKNLDFVAYKFENYGKEFIKKQKMSPDAYIQVALQLAFFRLNTKLVPTYESASLRRYECGRVDNIRSATPEALTFAKGMVDKKSGLQNSEKMQLFKSAITAQTNYTVLAITGMAIDNHLLGLRETARENFEELPDFFTDETYLASNRFVLSTSQVPTTMEMFCCYGPVIPQGYGACYNPQSQYILFCISSFKNCRETSSSQFVKAISESLMDLKELCSESSLTSKSQQAKLDGCPPVAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z8I6</accession>
    <name>A0A6I9Z8I6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">ICOS ligand-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC106557383</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013932090.1">
        <property type="nucleotide sequence ID" value="XM_014076615.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932091.1">
        <property type="nucleotide sequence ID" value="XM_014076616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z8I6"/>
    <dbReference type="GeneID" id="106557383"/>
    <dbReference type="KEGG" id="tsr:106557383"/>
    <dbReference type="OrthoDB" id="9904387at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF4">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN CD80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636671" evidence="13">
        <location>
            <begin position="32"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="33"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="151"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="310"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013932090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013932091.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="36127" checksum="8BEBC11872222822" modified="2020-10-07" version="1">MMHMQKTNPNLFSLKVFFWLCLFELLHSNSGTPSQKLRVVVQEGDEARLPCSHKIPEGSSPAYYYIYWQKPIPRITKMSDRVVISYKNGEEVESEKDSSYMNRSKIDKNFTLSLASVTVNDSGEYKCVALWPNNVQTLFVHLSVIVPFSTPIILSNLSSNLSSNLSSERCDSTNLTLICLSHGGSSEPKMYGSINEKPVNWTVTSNKIFGCFNITGTLQLSTTENILIQCSVHYLDFQVSTNYSLNVTNNCSEPTLPPLILPFWVLISSIVILVFFLAGVMLGILQCYVHKHRRSTTHKSLATDEMGLEEQSSGQKSSMLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZH7</accession>
    <name>A0A6I9WZH7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sesquipedalian</fullName>
            <shortName evidence="1">Ses</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PH domain-containing endocytic trafficking adaptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">FAM109B</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in endocytic trafficking. Required for receptor recycling from endosomes, both to the trans-Golgi network and the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sesquipedalian family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907529.1">
        <property type="nucleotide sequence ID" value="XM_014052054.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907530.1">
        <property type="nucleotide sequence ID" value="XM_014052055.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013907531.1">
        <property type="nucleotide sequence ID" value="XM_014052056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZH7"/>
    <dbReference type="GeneID" id="106537802"/>
    <dbReference type="KEGG" id="tsr:106537802"/>
    <dbReference type="CTD" id="150368"/>
    <dbReference type="OrthoDB" id="10261837at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13288">
        <property type="entry name" value="PH_Ses"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF15">
        <property type="entry name" value="SESQUIPEDALIAN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="1" id="KW-0967">Endosome</keyword>
    <keyword evidence="1" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="17"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013907529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013907530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013907531.1"/>
        </source>
    </evidence>
    <sequence length="269" mass="30305" checksum="52282F33816D6E77" modified="2020-10-07" version="1">MKLNERSVVHYATCDSPADHTGFLRKRMERHHHNHHVPSYQRRWFILKGNLLFYFEERESSDPLGLIVLEGCTVELCEAAEEFAFAIRFDSSGGKSYVLVADCQVAMEAWVKALSLASFDYMRLVVKELEKQLEEAQRSLAASHKSPKKLSSGRKRHLSNPMMISIQEHPIVLENGYSTWDNSCTLAEMACSDFSGGFLKPPPLPPRRKLTGSAGGGTFLAGSASATQESPVSPETACFSKLHNWYGQEIAKVRREWMERKKTVEIGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X030</accession>
    <name>A0A6I9X030_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-binding cassette sub-family C member 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106537946</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907726.1">
        <property type="nucleotide sequence ID" value="XM_014052251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X030"/>
    <dbReference type="GeneID" id="106537946"/>
    <dbReference type="KEGG" id="tsr:106537946"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF173">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="174"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013907726.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="37709" checksum="0342B0952EEB3EEA" modified="2020-10-07" version="1">MGYNSCTGEKIADGDYRTTTNCGGNAKYIPAIKHEGECTEVIHETGINLSGGQRQRICVARALYQNTNLVFLIIAMKDGTVLREGTLKDIQNKDVELYDHWKTLMNRQDQELEKVNVKNGGMKVWLDPDMLIFEEEEEEEEDDNMSAAAPLRTKMPWKTCWLYLTSGGFLLLSLMIFSKLLKHSVIVAIDFWLAQWTSTNQTQTLCTEKTCHVAVFSILSGAGIVLCLVTSLTVEWMGLTAAKNLYHDLLNKIILGPIRFFDMTPLGLILNRFSADTNIIDQHIPPTVESLSRSTLLCLSAVGMISYATHYFLLALLPLAVAFYFIQKYFRVASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0Y7</accession>
    <name>A0A6I9X0Y7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">POLR2G</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908909.1">
        <property type="nucleotide sequence ID" value="XM_014053434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0Y7"/>
    <dbReference type="GeneID" id="106538810"/>
    <dbReference type="KEGG" id="tsr:106538810"/>
    <dbReference type="CTD" id="5436"/>
    <dbReference type="OrthoDB" id="1162399at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="7">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013908909.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="20699" checksum="EF8ABDDCDE5ACA53" modified="2020-10-07" version="1">MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAIVTQVNKCLPNQDIVIQPEDNIRLKIVGTRVDKNDIFAIGSLMDDYLGTYSEFLAISLSLLPFGEPPFKKRGGVGNWEQDPDHKRSRSKISLTKARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X207</accession>
    <name>A0A6I9X207_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine-protein phosphatase non-receptor type</fullName>
            <ecNumber evidence="8">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PTPN14</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="8"/>
    <dbReference type="RefSeq" id="XP_013908361.1">
        <property type="nucleotide sequence ID" value="XM_014052886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X207"/>
    <dbReference type="GeneID" id="106538407"/>
    <dbReference type="KEGG" id="tsr:106538407"/>
    <dbReference type="CTD" id="5784"/>
    <dbReference type="OrthoDB" id="10012364at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001946">
        <property type="term" value="P:lymphangiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13188">
        <property type="entry name" value="FERM_C_PTPN14_PTPN21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17099">
        <property type="entry name" value="FERM_F1_PTPN14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000014">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000149">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000039">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014392">
        <property type="entry name" value="PTP_non-rcpt_14/21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041782">
        <property type="entry name" value="PTPN14/21_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF6">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000934">
        <property type="entry name" value="Tyr-Ptase_nr14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="13">
        <location>
            <begin position="21"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="773"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="12">
        <location>
            <begin position="980"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="569"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="9">
        <location>
            <position position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000934-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013908361.1"/>
        </source>
    </evidence>
    <sequence length="1077" mass="122484" checksum="45AD21EEC1086E41" modified="2020-10-07" version="1">MPFGLKLRRTRRYNVRSKNSFMTRIQLLENNLIECTLSVESTGQECLEAVGQKLELPETHYFGLWFHNKSQQVRWVELEKPLKKQLDKFASQSLLFFGVMFYVPSVALLQHEVTRYQYYLQVKKDVIDGRLRSSFEQGIRLAALAVQATLAAQVGFGDYPHFDSRDLRRYVLFPVDLVQNEAILEELIQKVVQEHRNHSNLSAAEAELLYIREVERLDGFGQDSFPVKDNQGNDFHLGIFCMGIFLKNPMGRSTVNYRWNDIGNITHNKSSIVVELVNKEETVLFHTDDLENAKYISRLFAAKHKFYKQNKICTEQSNSPPPIRRRPTWSRSSLPRQHTFIMPPVHVQCGEHYTETHNSQDSIFHGNEDMFYYRSQTSLDRCAMDFSYLNGSVPNGSVCSAQSMNSLNHSQNFVQASPVSSNLSIPGSDIMRADYIPSHRHSAIIVPSYRPTPDYDTVMRQMKRGVIHTDSQSQSLRNLNIGNTHVYSQPTDLVYSQPEIQERPSYTIAYGPQGTYKKHMTHSEQVNPMNALQAKPTSSAISHTVSTPELANMQLPNNQRCGPAQVLKNYLFRPPPPYPRPRPATSTPDLASHRHKYVTGSSPDLVPRKVQLSVKTFQEDSAPVVHQSLQEVSEPLMAAKHHAAINKRHSLEVISNMVRGMEAMALKSLNAPLPRRNTLRNEMQPEEVVQRGHEVQQLPHYHHKKTFSDATMLIHSSESEDEEEASESVPSMAALHENMEYSVQLQAALARIPNKPPPEYPGPRKSVSNGALRNDQINNVPRAVSRVKVARPGPARTISISRTDQNMMNGSSLGPSISEPDLTSVKERVKKEPVKERPVSEMFSIEDSIIEREMRYLEKQKMAGLEAQKRPLMLAALNGLSVARVPVPEDSQDEGAKVPMDERERGRTKSHRYWPILGSKHSSATYGKFKVTTKFRTVSGCYATTGLKVKHLGEAQEKTVWHLHYTNWPDHTCPGEQDFLSYLEEIQSVRRHTNSILDSSNNCNPPIVVHCSAGVGRTGVVILTELMIGCLEHNEKVDVPVMLQHLREQRMFMIQTIAQYKFVYQVLILFLQNSRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2H9</accession>
    <name>A0A6I9X2H9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="5">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CNOT7</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013908531.1">
        <property type="nucleotide sequence ID" value="XM_014053056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2H9"/>
    <dbReference type="GeneID" id="106538549"/>
    <dbReference type="KEGG" id="tsr:106538549"/>
    <dbReference type="CTD" id="29883"/>
    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000005">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013908531.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="32729" checksum="E9F23ED5DD06EFD6" modified="2020-10-07" version="1">MPAATVDHSQRICEVWACNLDEEMKKIRQVIRKFNYVAMDTEFPGVVARPIGEFRSNADYQYQLLRCNVDLLKIIQLGLTFMNEQGEYPPGTSTWQFNFKFNLTEDMYAQDSIELLTTSGIQFKKHEEEGIETQYFAELLMTSGVVLCEGVKWLSFHSGYDFGYLIKILTNSNLPEEELDFFEILRLFFPVIYDVKYLMKSCKNLKGGLQEVAEQLELERIGPQHQAGSDSLLTGMAFFKMREMFFEDHIDDAKYCGHLYGLGSGSSYVQNGTGNAYEEEANKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X681</accession>
    <name>A0A6I9X681_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Colipase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CLPS</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Enterostatin has a biological activity as a satiety signal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms a 1:1 stoichiometric complex with pancreatic lipase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909337.1">
        <property type="nucleotide sequence ID" value="XM_014053862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X681"/>
    <dbReference type="GeneID" id="106539162"/>
    <dbReference type="KEGG" id="tsr:106539162"/>
    <dbReference type="CTD" id="1208"/>
    <dbReference type="OrthoDB" id="9826993at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035473">
        <property type="term" value="F:lipase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23011">
        <property type="entry name" value="CLPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.80.10:FF:000005">
        <property type="entry name" value="Colipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047576">
        <property type="entry name" value="CLPS_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001981">
        <property type="entry name" value="Colipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017914">
        <property type="entry name" value="Colipase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017915">
        <property type="entry name" value="Colipase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017913">
        <property type="entry name" value="Colipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10041">
        <property type="entry name" value="COLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10041:SF9">
        <property type="entry name" value="COLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01114">
        <property type="entry name" value="Colipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02740">
        <property type="entry name" value="Colipase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00128">
        <property type="entry name" value="COLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00023">
        <property type="entry name" value="COLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57190">
        <property type="entry name" value="Colipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00121">
        <property type="entry name" value="COLIPASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51342">
        <property type="entry name" value="COLIPASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0222">Digestion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026662046" evidence="11">
        <location>
            <begin position="16"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Colipase N-terminal" evidence="12">
        <location>
            <begin position="21"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Colipase C-terminal" evidence="13">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909337.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="13620" checksum="3E7550BAE01F747A" modified="2020-10-07" version="1">MDRLWILSLLTFALAESTIHPRGLFINLENGELCLNSHQCKSHCCHRTTGLSLARCANKAAENQECSLKSLYGVYYKCPCEEGLICDADRTIVGSIVNSDFGICKDPSSKISSDKKERKKYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6F4</accession>
    <name>A0A6I9X6F4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Transcription elongation factor A N-terminal and central domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">TCEANC2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911039.1">
        <property type="nucleotide sequence ID" value="XM_014055564.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911040.1">
        <property type="nucleotide sequence ID" value="XM_014055565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6F4"/>
    <dbReference type="GeneID" id="106540461"/>
    <dbReference type="KEGG" id="tsr:106540461"/>
    <dbReference type="CTD" id="127428"/>
    <dbReference type="OrthoDB" id="44867at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF14">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR A N-TERMINAL AND CENTRAL DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="41"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="6">
        <location>
            <begin position="132"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013911039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911040.1"/>
        </source>
    </evidence>
    <sequence length="209" mass="24147" checksum="AE27562946F6AB8F" modified="2020-10-07" version="1">MMDKFVIRTPRTKGESTKNDPGEKVYKQATIESLKRVVVVEDIKRWKSILELSIQPKENLIEALMELKKKIPSKEILLSTKIGHTVNKMRKHSDQDVATLAKEVYTEWRSFIKNHSNRPLIEVRSNPRTEGLRQNARKLLAEALELEIGHLLAENIEQEAFYLSSHLVSTSYRRTVRALVFSLKHNPETRAQVQSGVLSVKMLVRNHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8Y8</accession>
    <name>A0A6I9X8Y8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bcl-2 homologous antagonist/killer</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Apoptosis regulator BAK</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">BAK1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19 20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912039.1">
        <property type="nucleotide sequence ID" value="XM_014056564.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912040.1">
        <property type="nucleotide sequence ID" value="XM_014056565.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013912041.1">
        <property type="nucleotide sequence ID" value="XM_014056566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8Y8"/>
    <dbReference type="GeneID" id="106541202"/>
    <dbReference type="KEGG" id="tsr:106541202"/>
    <dbReference type="CTD" id="578"/>
    <dbReference type="OrthoDB" id="6020735at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097145">
        <property type="term" value="C:BAK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034644">
        <property type="term" value="P:cellular response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.437.10:FF:000007">
        <property type="entry name" value="bcl-2 homologous antagonist/killer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020717">
        <property type="entry name" value="Bcl2_BH1_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020726">
        <property type="entry name" value="Bcl2_BH2_motif_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF41">
        <property type="entry name" value="BCL-2 HOMOLOGOUS ANTAGONIST_KILLER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01080">
        <property type="entry name" value="BH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01258">
        <property type="entry name" value="BH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="16">
        <location>
            <begin position="82"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013912039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013912040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013912041.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24809" checksum="9624385262C2A77A" modified="2020-10-07" version="1">MASWNGSNSADRRRTGERRRSSQETTSEDLVAQQTEEVFQSYAFHRYQQEQEQTEGDVPVDPEIAAIQQEPNSINSQVGRRLAIIADDINARYDKEFSEMLKPLQLSKDNAYEYFTRIASSLFESGINWGRVIALLGFGYRMAIHVYQHGITGFLRRIAHYMAEFVLHNRIAQWIAQQGGWVAALDLSNVYWKYMLMVATVILLGQFVLRRFFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBQ4</accession>
    <name>A0A6I9XBQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Biotin--protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HLCS</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911711.1">
        <property type="nucleotide sequence ID" value="XM_014056236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBQ4"/>
    <dbReference type="GeneID" id="106540930"/>
    <dbReference type="KEGG" id="tsr:106540930"/>
    <dbReference type="CTD" id="3141"/>
    <dbReference type="OrthoDB" id="10250105at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026868349" evidence="4">
        <location>
            <begin position="20"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="BPL/LPL catalytic" evidence="5">
        <location>
            <begin position="640"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911711.1"/>
        </source>
    </evidence>
    <sequence length="911" mass="101858" checksum="CC80315BD8E57DB7" modified="2020-10-07" version="1">MLITLCYLYLWARWRPSSAGLIRRTVRRLYRTRCSFTFCSSSGCSSCSSFGCTRFTLHQQVPHLPLCKQEVPGLSDEIVYLRVGDKVFFTGETQSLDDLNKWTLFIVSHFNYLEQVVEDEHITFVTEGIFIQTSSSQAITCSANKIVKWSDFCSPLAFKTGKPYILFAEASIDNFSSLGIAFLENRLQMDNGMVPQKIVSVNLKKSALKELKLAPGTKEEEKSGQIQTSELPQDSLFENIDKSQVETAEFTGSGDQEMHQEKCRLQKTNGYHEESEQLSEKEGDASEHHHLHLSSCHECLELENSTIESVKFASAENIPELPDDYSGSVEEVSGDYITGNMQRVNLTGKPPNILIYLGSDSKKIKFEEIKSIIMDCVDINAYTIYQLLEKQVLTVPWVDNALLLIIAESEPISDAVSKQFLAFMSKGGKILGLSASFTFGGVKLKSKNEIMDTIRTVVFSKDNNEIKLNALASGKIFEVDISEKLNPIKPLGYFDGPDKDMMIVHLSYGNNGGEAILSQAHLEVNIKSLCRPKDDFNLLKISNTKRYDVLVEILKLLGLSCELSTIPSLTPLYLLSFDKILHNTFLEWLRRNTITEGLITSSKMSLKFVSSCTENMEITPLLIPVVTDTEAFSSENFSFERYKQNLGSRILGKIVLFSEVTSTTMNLLDGLMYKTPQKMGLIAISVQQIQGKGRGGNTWLSPVGAALSTLHVIIPLSSKLGQRIPFIQHLVSLAIVEAVRSIPGYQEIDLRLKWPNDIYYSDLMKLGGVLVNSTLTGDTFHILIGFGINVNNSNPTVCINDLIVEYNKKMNTTLEPLNADSLIARSVTVLENLINIFQEKGPNGILPIYYKYWVHSGKQVRLGNDEGPLVWIVGIDDSGFLQVYEEGKDVITVHPDGNSFDMLKNLIIPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCC7</accession>
    <name>A0A6I9XCC7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">CREB3 regulatory factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">CREBRF</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5 6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913239.1">
        <property type="nucleotide sequence ID" value="XM_014057764.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913240.1">
        <property type="nucleotide sequence ID" value="XM_014057765.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913241.1">
        <property type="nucleotide sequence ID" value="XM_014057766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCC7"/>
    <dbReference type="GeneID" id="106542117"/>
    <dbReference type="KEGG" id="tsr:106542117"/>
    <dbReference type="CTD" id="153222"/>
    <dbReference type="OrthoDB" id="8931646at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14809">
        <property type="entry name" value="bZIP_AUREO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039165">
        <property type="entry name" value="CREBRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21552">
        <property type="entry name" value="ADULT RETINA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21552:SF2">
        <property type="entry name" value="CREB3 REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="553"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013913241.1"/>
        </source>
    </evidence>
    <sequence length="665" mass="75118" checksum="DD4279934B8D3F8A" modified="2020-10-07" version="1">MSYMLPTNPEKNLEQNLQARKTKKLELNLEDLGLEMPQPSVSGMDPPFGDAFQSHMFSEQRLISTDLLANNSDPDFMYELDRGMDCQQSSRDNLLSVEDCKEFENLETLTDILDTEMSFPANWDQWDTYCEDLTKYTKLTSSDIWGTKEVDYLGLDDFSSPYQDEEVISKTPTLAQLNSEDSQPVSDPLCYSELLFNAKQTQLTSLPTKKITRAATPVCSSKTAQAEAPLSEFAHKASKANSSTQIMVKTNMYNNEKANIHIECKDYVKKAKVKINPMHQNRSALSQANADAAKENTCYCGVVAKRQEQKGIDPLQNHSAPVLPFKETQALLLTPPQEAPGLIAEKSNLSASTSVSDPSQKKEEHNYSLFVSDGSADHAGTADPDEDEDDEEEVEDEDHDEGFGSEHELSENDDEEDYEDDKDDDISDTFSEPGFENDSVEDLKEMTAISCRKRGKRRYFWEYSEQLTPSQQERMLRPSEWNRETLPSNMYQKNGLHHGKYAVKKSRRTDVEDLTPNPKKLLQIGNELRKLNKVISDLTPVSELPLTARPRSRKEKNKLASRACRLKKKAQYEANKVKLWGLNTEYDNLLFVINSIKQEIVNRVQSPKDERVTNMSQKLDVLIKDTLGQPVSGQTSEFVNQVLEKTAEGDPTGGLVGLRIPTSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD47</accession>
    <name>A0A6I9XD47_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Exostosin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106538947</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909078.1">
        <property type="nucleotide sequence ID" value="XM_014053603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD47"/>
    <dbReference type="GeneID" id="106538947"/>
    <dbReference type="KEGG" id="tsr:106538947"/>
    <dbReference type="OrthoDB" id="5954868at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF97">
        <property type="entry name" value="EXOSTOSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="3">
        <location>
            <begin position="111"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013909078.1"/>
        </source>
    </evidence>
    <sequence length="475" mass="55073" checksum="5EB3F4CBF211827A" modified="2020-10-07" version="1">MQAKKRYFTLLSAGTFVVLLFYLGGLQFRASRSQSRREARSSGGRNGLQRPERYWPRFPDALRPFLPWDQLESEDYNRHASPRQKRDANAGLYKGKKCRMESCFDFALCRKNGFKVYVYPPQKGEKIAESYQNILAAVEGSRFYTSDPSQACLFVLGLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLVFNLYSGTWPDYTEDVGFDIGQAMLAKASISTENFRPKFDVSIPLFSKDHPRTGGERGFLRFNTIPPLRKYMLVFKGKRYLTGIGSDTRNALYHVHNGEDVVLLTTCKHGKDWQKHKDSRCDRDNAEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEALQAACIPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XD88</accession>
    <name>A0A6I9XD88_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PR domain zinc finger protein 10-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106542396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913584.1">
        <property type="nucleotide sequence ID" value="XM_014058109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XD88"/>
    <dbReference type="GeneID" id="106542396"/>
    <dbReference type="KEGG" id="tsr:106542396"/>
    <dbReference type="OrthoDB" id="3535323at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913584.1"/>
        </source>
    </evidence>
    <sequence length="266" mass="28546" checksum="0BF5445474D488E0" modified="2020-10-07" version="1">VKTSDLLTQAMTELSQTLTTDYRTSQGDFPRIQYIPVSQSAAGLQQPQHIQLQVVQVAQATSPHQTQHSTVDVGQLHDPQSYTQHAIQVQHIQVTEPTSAAPSSSQVGSQPLSPSQPTQQELSPSQIQTTSSGQSQSQAGSSVQQAYLPNTWNSFRTYPSEIQMMTIPQGQYVIAEAAVGPPVTTVTTGQVKAVSQTHYVIAESQPELEVKPSLSLSSGVEVDLSQTPVHADSLDSQTASEQQTTQYIITATSNTNGVGDLHIEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDG3</accession>
    <name>A0A6I9XDG3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Leucine-rich repeat-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LRRC4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913669.1">
        <property type="nucleotide sequence ID" value="XM_014058194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDG3"/>
    <dbReference type="GeneID" id="106542460"/>
    <dbReference type="KEGG" id="tsr:106542460"/>
    <dbReference type="CTD" id="64101"/>
    <dbReference type="OrthoDB" id="28057at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904861">
        <property type="term" value="P:excitatory synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097119">
        <property type="term" value="P:postsynaptic density protein 95 clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000012">
        <property type="entry name" value="Leucine rich repeat containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026854032" evidence="15">
        <location>
            <begin position="37"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="351"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913669.1"/>
        </source>
    </evidence>
    <sequence length="656" mass="72013" checksum="525630973F0E22EA" modified="2020-10-07" version="1">MKLLRQAPPQPARNAAPLACLAYLVAQAWLVACAVAARPGPQSCPSVCSCSNQLSKVVCTRRGLAEVPPGIPSGTRYLNLMENGIKVIQADTFRHLHHLEVLQLGRNAIRQIEVGAFNGLASLNTLELFDNWLTVVPSGAFEYLSKLRELWLRNNPIESISSYAFNRVPSLMRLDLGELKKLKYISEGAFEGLYNLKYLNLGMCAIREMPNLSPLVGLEELEMSGNTFLAIKPGAFHGLKSLKKLWIMSAQIGAIERNAFDDLTALVELNLAHNNLTSLPHDLFAPLRYLVELHLHHNPWNCDCDILWLSWWLREYIPTNSSCCGRCHAPTHMRGKFLVEVDQTAFQCSAPFILDAPRDLNISEGRVAELKCRTPAMSSVRWLLPNGTVLSHASNHPRISVLNDGTLNFSQVLLTDTGTYTCMVTNVAGHSNASAYLNVSTAELNTSNYSFFTTVTVETTETSPENLFPKFTKPVPTASTGYQPAYTTTTTVLVQTTKTPRQDATLDGNDKMQTSLDEVMKTTKIIIGCFVAVTLLAAAMLVAFYKLRKRHQQRSTVGAARTVEIIQVDEDIPASAGAAPTTATTAATPSTASGLSGEGAVVLPAIHDHYNTYKPSQGAHWTENCLGNSLHPTVTTIAEPYIIQTHPKEKVQETQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDV8</accession>
    <name>A0A6I9XDV8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription factor ETV6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ETV7</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Can form homodimers or heterodimers with TEL2 or FLI1. Interacts with L3MBTL1 and HDAC9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912052.1">
        <property type="nucleotide sequence ID" value="XM_014056577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDV8"/>
    <dbReference type="GeneID" id="106541206"/>
    <dbReference type="KEGG" id="tsr:106541206"/>
    <dbReference type="CTD" id="51513"/>
    <dbReference type="OrthoDB" id="10042983at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08535">
        <property type="entry name" value="SAM_PNT-Tel_Yan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000030">
        <property type="entry name" value="transcription factor ETV6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000176">
        <property type="entry name" value="transcription factor ETV6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF77">
        <property type="entry name" value="TRANSCRIPTION FACTOR ETV7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02198">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PNT" evidence="17">
        <location>
            <begin position="31"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="16">
        <location>
            <begin position="221"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013912052.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="36178" checksum="55CE9F6B5BF6D189" modified="2020-10-07" version="1">MQVEVTAGSFTPVATSSLPLPLHSTLKTSVKSLYDGQICTLPGRLRIQPFLWSKDDVIHWLRWAEKEYSLRKSDDSNFEMNGKALCILTKEDFKFRAPNSGDVLYELLQYIKTQRRALVCSPLFNISFWDIDHNLTQSTAEGTKCNLEDFPDKVPALPLEYPTIFTSHASYSDSSQSSAKTLCTPGTPLVYSNPEISHCGNICSFPVKSAAPVSGKISDCRLLLDYIYRLLSDSQYESFIKWEDKETKIFRVVNPNGLAGLWGNQKNRRNMTYEKMSRALRHYYKLNIIKKETGQKLLFRFLKVPGEIKQYTASKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XF00</accession>
    <name>A0A6I9XF00_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 112</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CCDC112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013912452.1">
        <property type="nucleotide sequence ID" value="XM_014056977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XF00"/>
    <dbReference type="GeneID" id="106541503"/>
    <dbReference type="KEGG" id="tsr:106541503"/>
    <dbReference type="CTD" id="153733"/>
    <dbReference type="OrthoDB" id="2152435at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039902">
        <property type="entry name" value="CCDC148/CCDC112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21549:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21549">
        <property type="entry name" value="MUTATED IN BLADDER CANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="295"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="379"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="127"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013912452.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="59531" checksum="A2881609AFB8C042" modified="2020-10-07" version="1">MAALATPTLEGWTRYQQNGCFSASDALQHFQSWKTKTDQVKKTEFIRTGNKLKNQLVNLEKDKHVLLFNKKSDFRMEYCILEELEHKLTKNRKTEKAKIQQKLTRIHSAVKGLQRQLKDVKPTPEFVDKLREMMEEAETAISSFKEEQRQIYDELLREENAATNEISALERKIEMWAVGTSAARIFKQCVFPVHKEVQNQFPEEVLELERFLQQTGGRQGGWDECDHQIFRKIWRKHKGKPSYITETLEYLPDRTKEDIQSHEKWHKEFLILESKKKEAIKKWKMKKQERGKILVQKENPEEVPADIQREREEAQKQKTEEERKKRLAELEAWKRDKALATAAKQEAQFKEEEEKEKKKQRERQRQLQTKLLLEDYSREKKEQEDLLRLEKERREEAEREEKKRTAAEEIVKFQERDLHKLELKFLEKQAKEDEKIEKEKRLSKLRKKVHINVTRDPCRLFRPTKVWEERTKEIGPIGTGPVLYIPHRAVPSWRQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG70</accession>
    <name>A0A6I9XG70_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane channel-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMC1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013912867.1">
        <property type="nucleotide sequence ID" value="XM_014057392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG70"/>
    <dbReference type="GeneID" id="106541850"/>
    <dbReference type="KEGG" id="tsr:106541850"/>
    <dbReference type="CTD" id="117531"/>
    <dbReference type="OrthoDB" id="9397520at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050910">
        <property type="term" value="P:detection of mechanical stimulus involved in sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060005">
        <property type="term" value="P:vestibular reflex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF18">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013912867.1"/>
        </source>
    </evidence>
    <sequence length="444" mass="52320" checksum="17922BD6308B6B7A" modified="2020-10-07" version="1">MVSPNQNDSPQSRGEIYLPLLLFGSTERQNDIQADVTEYPVFTVYDICFFSTVDIEGHNEEDNATESEEEEQSEQKKKASKRKKNETEEEDEDEEETAAERRRRYRRRRRKDGNEGSESEGEAHKTRKNRENQKIRDESREDDDEEDEDKRSKDKKKEKKKKKNQDKKRKGSSSDSTSEEEMTEEELAKLKEAVEEKKKLISTLRNKPWNMKKKLKVLKDAQRFVEKFEGALGKGKGKKFYAYKVMMMKKWMKFQRDFENFRTACIPWEMKIKEIESHFGSSVASYFIFLRWMYGINIVLFGLTFGLVMVPEALMGKAYGSLPRKTVPRAEEASAMNFATLWDFSWILKDTMRKTMLLKVKKRNSLNKRKKHQKERKMKPRKRMRMKKRQLQNAGEDTGDDGGKMGMKVVNPRGKPTRPGRTEKIRRLGMSPGKTTMKRMRTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHD7</accession>
    <name>A0A6I9XHD7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Asparagine-linked glycosylation protein 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ALG5</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013913282.1">
        <property type="nucleotide sequence ID" value="XM_014057807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHD7"/>
    <dbReference type="GeneID" id="106542155"/>
    <dbReference type="KEGG" id="tsr:106542155"/>
    <dbReference type="CTD" id="29880"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000068">
        <property type="entry name" value="ALG5, dolichyl-phosphate beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="16">
        <location>
            <begin position="63"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913282.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="36356" checksum="E94DCF389EB20088" modified="2020-10-07" version="1">MELGLLIEGIVVLATAGLALVAAIAYITARQSPSIYEYEGENLFLNDKKPVPSLHDPATRELSVVVPSYNEEERLPSMMNEALDYLEMRQKQDPSFTYEVIVVDDGSTDQTTKVALRYVEKYGSDKVRVLTLVRNRGKGTAVRMGVFYSRGKRILMADADGATKFKDIEKVEKGLESLQPWPEMMAIACGSRAHLEKDSIAQRSYFRTFLMHGFHFLVRFLCVKDIRDTQCGFKLLTREAASRTFSALHIERWAFDVELLYIAQSFKIPISEIAVNWTEIEGSKLVPFWSWLQMGKDLLFIRLRYLTGAWKLDTRKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHH5</accession>
    <name>A0A6I9XHH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Muscarinic acetylcholine receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">CHRM2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Postsynaptic cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913546.1">
        <property type="nucleotide sequence ID" value="XM_014058071.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913547.1">
        <property type="nucleotide sequence ID" value="XM_014058072.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013913548.1">
        <property type="nucleotide sequence ID" value="XM_014058073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHH5"/>
    <dbReference type="GeneID" id="106542367"/>
    <dbReference type="KEGG" id="tsr:106542367"/>
    <dbReference type="CTD" id="1129"/>
    <dbReference type="OrthoDB" id="10071887at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008016">
        <property type="term" value="P:regulation of heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006940">
        <property type="term" value="P:regulation of smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15297">
        <property type="entry name" value="7tmA_mAChR_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000038">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000041">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001065">
        <property type="entry name" value="Musac_Ach_M2_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000995">
        <property type="entry name" value="Musac_Ach_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF207">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00243">
        <property type="entry name" value="MUSCARINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00539">
        <property type="entry name" value="MUSCRINICM2R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 12 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0770">Synapse</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="42"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="220"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="279"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013913546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013913547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013913548.1"/>
        </source>
    </evidence>
    <sequence length="468" mass="51711" checksum="7888C67E0E7E1D67" modified="2020-10-07" version="1">MNNSTNISYLEDRMTLPSPYKTVEVVFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVTGSWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVRRTTKTAGMMIAAAWVLSFILWAPAILFWQFIVGERTVPDKECYIQFLSNAAVTFGTAIAAFYLPVVIMSILYWQISRASRSRVKKDKKGGAQNQEPASPSLVQGKIGKPNNNNLPTNEDGTEHNKILNGKAAGEPVMENCVPAEEKESSNDSTSVSAGASNMKEDEAITENPRASVSQVNAKVENSKLTCIRILTKSPKGDYGGPTNTAVEIVATDGQNGEEKQNIVARKIVKMTKQPPKKKAPPSREKKVTRTILAILLAFIITWTPYNVMVLINSFCSSCIPNTVWTIGYWLCYINSTINPACYALCNATFKKTFKHLLMCNYKNIGATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHX2</accession>
    <name>A0A6I9XHX2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Intraflagellar transport protein 74 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">IFT74</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5 6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910551.1">
        <property type="nucleotide sequence ID" value="XM_014055076.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910552.1">
        <property type="nucleotide sequence ID" value="XM_014055077.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013910553.1">
        <property type="nucleotide sequence ID" value="XM_014055078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHX2"/>
    <dbReference type="GeneID" id="106540080"/>
    <dbReference type="KEGG" id="tsr:106540080"/>
    <dbReference type="CTD" id="80173"/>
    <dbReference type="OrthoDB" id="444379at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029602">
        <property type="entry name" value="IFT74"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31432">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31432:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013910551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013910552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013910553.1"/>
        </source>
    </evidence>
    <sequence length="599" mass="68861" checksum="D42FA635A1EB489A" modified="2020-10-07" version="1">MASNHKPPTARPSSRIGLSARPPSSSLRPPSAALLGGGMPPGTSRPGTRGGSIGTGVVLSSQIKVADRPVTQQGLSGMKTGMKGPQRQIMDKSYYLGVLRGKINELSAEINKLQKEVDMYNQENSVYLSYEKRAESLASEIKEFQGQLADYNMLVDKLNTNTEMEEVMNDYNLLKAQNDREAQSIDIIFTERQSKEKLIQAVEDDIRQEKQAAENIIKNMSEEDQTKYIDMKMANEKLLKELSSLQQEMDAVNMKEQALEAEITHSQIKQEALQLYEKLHGLEEHRDQMIAEDKNMGSPQEERERLLKQVKEDNQEIASMERQLTEIKEKSNHLNEGIRQLDMDLEEHQGEKNLKYKELKKREESMDNFLESFEETKNQELERKSEIEANIVALLEHSSRNVSRMKQISSVTNQELKIMQEDLTFKSSEMQKSQSTAKNLGTDSQRLQMDLQKMGLLEGKMVDELTSLKEKIEQMTKDLEVYNNLPALKTAGEEKKKKLQEEKERLIKHRDAFKKIMEHLNKEYEGLKLQLQENETHAQLTNLERKWQHHEQNNFVMKEFIATKSQESDYGPIMNDVMKEVAEYNKTIIEALQSSKNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XI53</accession>
    <name>A0A6I9XI53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Gastrotropin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FABP6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915439.1">
        <property type="nucleotide sequence ID" value="XM_014059964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XI53"/>
    <dbReference type="GeneID" id="106543836"/>
    <dbReference type="KEGG" id="tsr:106543836"/>
    <dbReference type="CTD" id="2172"/>
    <dbReference type="OrthoDB" id="10016075at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000006">
        <property type="entry name" value="Fatty acid-binding protein, liver"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14651">
        <property type="entry name" value="Lipocalin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="6">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013915439.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14694" checksum="CA6818649CA1EAA1" modified="2020-10-07" version="1">MAFTGKYEMENEENYDNFMKRLGLPHDTIEKGRNFKIISEIVQNNDEFTWSQIYPGGHTLTNTFVIGKESEMETVGSKKFKAIVRKENGKLIADFPNYRYTAEIAGDKLIEVSTVGDITFKRISKKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJS9</accession>
    <name>A0A6I9XJS9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BCCIP homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">BCCIP</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the BCP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914271.1">
        <property type="nucleotide sequence ID" value="XM_014058796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJS9"/>
    <dbReference type="GeneID" id="106542926"/>
    <dbReference type="KEGG" id="tsr:106542926"/>
    <dbReference type="CTD" id="56647"/>
    <dbReference type="OrthoDB" id="27543at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028983">
        <property type="entry name" value="BCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013914271.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="35471" checksum="1BF14CE85731A04B" modified="2020-10-07" version="1">MATEAKRRKADGPSKSRLVEEEEEAEEEEEGSEDGDSDSELESDSEEDDEEINEEVNIEFEAHTVTDDDKNGINKLLQQLFLKAPINTGNLTDILIQQNHIGSVIKQTEVTEESDDEDNDEVFGFISLLNLTERKGTECAEQIKELVLSQCEKNCEQNVVEQLDKLLSDTTKPVGFLLSERFINVPPQIALPMHQQLQKEIVEAQRTNKPCGKCYFYLLISKTYTHKKNAKKRGSGTHQKTELMFSNAEEEFFYEQSVLKFSYSVEEESDSCLGGRWSFEDAPMKPLRTVMVIPADRMSAIMEKFKEYLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKD0</accession>
    <name>A0A6I9XKD0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106544496</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013916254.1">
        <property type="nucleotide sequence ID" value="XM_014060779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKD0"/>
    <dbReference type="GeneID" id="106544496"/>
    <dbReference type="KEGG" id="tsr:106544496"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF12">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="710"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013916254.1"/>
        </source>
    </evidence>
    <sequence length="808" mass="89047" checksum="D519535C7CB6EC60" modified="2020-10-07" version="1">MVCLVLNAQARTHHTCQNQFMMSTEAPLWSQRATIEAVAEKKQVMIDENLVEQFVCGVKDIHLRRRLLWHPDITMQQAVDEARAAELSEQSTADIQRMQPLPPSPPALPVCPPPAMQACLIDELVLLEDPSSEQVVQLRAQPRRPPPPPAAPATSGCVGCGSCHARTACPFKGMVCHRCGKKDHIAQVCRASLPAPSFLQKHAAPQTQPCHQPPRPARRAEDCYAVNQPDVAGETLSQASAGAQKVSAVLHLEGQPCRMEVDSGSFRSLLSWATFSRLCPHISCDQLQPVDTSLRDYQGTLIPTLGSFPIWVDYGDFHGRLPVLIVKNSQPALLGLDWFPSLGLSIGGLHRVAAYSLEDVLTEFADIFDGQLGSYKGTPISLNLDPQVAPIQLKARRVPFALRAKVDAELDKLLAQGVLEPVDHSRWETPIVVPVKPDGSVQICADSKSTINFALQANPYPVPVVQHLLHSLEQGCTFAKLDMAQSYQQLPVDGNVAAAQTIVTHRGAFRCRRLQFGISLAPGIFQSLMERLLHGLPGMVPYFDEVLIAASSHSKLIEMLRKVLLRFRGVGLKLKRRKCSFAVPRVEFLGFLIDTQGIHPTPAKVCSWVLRGWPLGKVADSFRPFKARQAELSLHGGCLIWGNWVVIPSTLRAQVLPLLHKDHAGIAQMKVLARSYVWWCLLDSEIAAYVGRCTTCQLSRPNPPAGPSREWEGPRGLWSRLHVDFAGPFHGQTFLIVVDAYSRWVELVLMSSTSAESMVRDLRRLFATHGLPDIIVSDNGPQFTSTTFQEILACQGIQHVPTAPYHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XM60</accession>
    <name>A0A6I9XM60_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hepatic leukemia factor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HLF</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. PAR subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916969.1">
        <property type="nucleotide sequence ID" value="XM_014061494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XM60"/>
    <dbReference type="GeneID" id="106545043"/>
    <dbReference type="KEGG" id="tsr:106545043"/>
    <dbReference type="CTD" id="3131"/>
    <dbReference type="OrthoDB" id="6022300at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000007">
        <property type="entry name" value="hepatic leukemia factor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF28">
        <property type="entry name" value="HEPATIC LEUKEMIA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="223"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="255"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013916969.1"/>
        </source>
    </evidence>
    <sequence length="293" mass="33270" checksum="DC4DB33531281A73" modified="2020-10-07" version="1">MARPLPLNPAFLPPTYGVLKSLLENPLKLPLHHEDAFYKEKDKDKKLDDDSTSATVPQSAFLGPTLWDKTLPYDGDTFQLEYMDLEEFLSENGIPPSPVQHDHSPHQPALQQPTSASPSVMDLSHRASTSVHPTMVSQNCMQNTARPGQILPTHRNTPSPIDPDSIQVQVGYEPDPADLALSSIPGQEIFDPRKRKFSEEELKPQPMIKKARKVFIPEDLKQDDKYWARRRKNNMAAKRSRDARRLKENQIAIRASFLEKENTALRQEVADLRKELGKCKNVLVKYEARHGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XMF7</accession>
    <name>A0A6I9XMF7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bile salt-activated lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CEL</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915092.1">
        <property type="nucleotide sequence ID" value="XM_014059617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XMF7"/>
    <dbReference type="GeneID" id="106543569"/>
    <dbReference type="KEGG" id="tsr:106543569"/>
    <dbReference type="CTD" id="1056"/>
    <dbReference type="OrthoDB" id="19653at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="60"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="165"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013915092.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="32978" checksum="E5FCF7E29D35CB73" modified="2020-10-07" version="1">MIWIYGGGFIWGSGQGANFLSNYLYDGEEIASRGKVIVVTFNYRLGPLGFLSTGDAALPVFGESAGAASVSLQTLSPYSKGLMKRAISQSGVGLCSWAIQKDPLYWAIKTAQKVGCPTDNTTVMANCLRITDPKTLTLAYHLELTNLRCKTEWERASGRLEDEITGKTYSYLFSEPSRMPVYPGWVGADHADDIQYVFGKPFATPLGYRARHRTLSKAMIAYWTNFARTGDPNRGESEVPIAWVPYDKENSFYLDINHDINYNSVKQGLRDVYVKFWNVAYRNLPNVRTAEKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XN10</accession>
    <name>A0A6I9XN10_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Centriole, cilia and spindle-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCSAP</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013915306.1">
        <property type="nucleotide sequence ID" value="XM_014059831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XN10"/>
    <dbReference type="GeneID" id="106543755"/>
    <dbReference type="KEGG" id="tsr:106543755"/>
    <dbReference type="CTD" id="126731"/>
    <dbReference type="OrthoDB" id="6616361at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901673">
        <property type="term" value="P:regulation of mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029774">
        <property type="entry name" value="CSAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31022">
        <property type="entry name" value="CENTRIOLE, CILIA AND SPINDLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31022:SF4">
        <property type="entry name" value="CENTRIOLE, CILIA AND SPINDLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15748">
        <property type="entry name" value="CCSAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013915306.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="31000" checksum="6DCD3AFF14029A98" modified="2020-10-07" version="1">MVPSRCVKSEYMKRFKEPKWEACGPCYQELLRYRLSRRLLEQAHRPWFWDDWEQDISLNGSSGSGSSQSSQGTSSPLVVRQPEEEAAHPAGEIEIPGGDLGLNRISAAGEDVASSPAPAEPPGGHEVDVQEQSEGKAIVKKDERKTAGKGIDSSSCPQKQPLSQKSASSRQGDRKGAKNPQRTETTLEIKHPFALYGWGEKQTDTGSQKTHNVRASASVNEIHESALRAKNRRQVEKRKLPQRRVHPAEVERTWTTKPSGPDSPWVTEYMRCYSARAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XNC0</accession>
    <name>A0A6I9XNC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NHP2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917434.1">
        <property type="nucleotide sequence ID" value="XM_014061959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XNC0"/>
    <dbReference type="GeneID" id="106545408"/>
    <dbReference type="KEGG" id="tsr:106545408"/>
    <dbReference type="CTD" id="55651"/>
    <dbReference type="OrthoDB" id="5364946at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000016">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="46"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013917434.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17205" checksum="A015BA975CEB1AFD" modified="2020-10-07" version="1">MGRVKHQEPEEAPETEATTPERSYHELVANVNAIARPLASRKLTRKLYKCIKKASKLKQIRRGVKEVQKFINKGEKGITVLAGDTLPIDVYCHIPIMCEDRSLPYVYIPSKTDLGAAAGSKRPTCVIMIKAHEDYQEAYNECLEEVEALPLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP05</accession>
    <name>A0A6I9XP05_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lupus La protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106543996</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013915601.1">
        <property type="nucleotide sequence ID" value="XM_014060126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP05"/>
    <dbReference type="GeneID" id="106543996"/>
    <dbReference type="KEGG" id="tsr:106543996"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12541">
        <property type="entry name" value="RRM2_La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014886">
        <property type="entry name" value="La_xRRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08777">
        <property type="entry name" value="RRM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51939">
        <property type="entry name" value="XRRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="XRRM" evidence="4">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013915601.1"/>
        </source>
    </evidence>
    <sequence length="185" mass="20698" checksum="545D6A935DFDB3CE" modified="2020-10-07" version="1">KYLAEKQGCLLKFFGDLEDHTCREDLHAVFSNHGEIKWIDFVRGAKEGIILFKSSAKGALDKAQEANNGKLQLREKDVTWEVLEGDLAKEALRKIMESQQELLNRKRGKGRKGKGKGGKGAQDTQDKKVKFQGKKTKFESDDEEEGEDAIITESASPKKRPLEEDVQEEPAAKQLKTENGAGIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XP34</accession>
    <name>A0A6I9XP34_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Microtubule-associated proteins 1A/1B light chain 3C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MAP1LC3C</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912478.1">
        <property type="nucleotide sequence ID" value="XM_014057003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XP34"/>
    <dbReference type="GeneID" id="106541518"/>
    <dbReference type="KEGG" id="tsr:106541518"/>
    <dbReference type="CTD" id="440738"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17236">
        <property type="entry name" value="Ubl_ATG8_MAP1LC3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000149">
        <property type="entry name" value="microtubule-associated proteins 1A/1B light chain 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027731">
        <property type="entry name" value="MAP1A/MAP1B_LC3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="9">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013912478.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16623" checksum="32E780A56B750751" modified="2020-10-07" version="1">MQPLQQNCQPVRPFKQRKSFATRVEEVAGIRGKFPTKVPVIVERYQKEKYLPLLDKTKFLVPQELTMTQFITIIRSRMALSATQAFYLLINNKSLASMSLTLAEVYQDYKDEDGFVYMTYASQEMFGGLTTSKVKCKEYLLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ31</accession>
    <name>A0A6I9XQ31_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fascin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106545943</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the fascin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918144.1">
        <property type="nucleotide sequence ID" value="XM_014062669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ31"/>
    <dbReference type="GeneID" id="106545943"/>
    <dbReference type="KEGG" id="tsr:106545943"/>
    <dbReference type="OrthoDB" id="9928158at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031253">
        <property type="term" value="C:cell projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23334">
        <property type="entry name" value="beta-trefoil_FSCN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23335">
        <property type="entry name" value="beta-trefoil_FSCN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23336">
        <property type="entry name" value="beta-trefoil_FSCN_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23337">
        <property type="entry name" value="beta-trefoil_FSCN_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000008">
        <property type="entry name" value="Fascin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000010">
        <property type="entry name" value="Fascin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000015">
        <property type="entry name" value="Fascin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010431">
        <property type="entry name" value="Fascin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022768">
        <property type="entry name" value="Fascin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024703">
        <property type="entry name" value="Fascin_metazoans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10551">
        <property type="entry name" value="FASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10551:SF39">
        <property type="entry name" value="FASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06268">
        <property type="entry name" value="Fascin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005682">
        <property type="entry name" value="Fascin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fascin-like" evidence="7">
        <location>
            <begin position="13"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Fascin-like" evidence="7">
        <location>
            <begin position="184"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Fascin-like" evidence="7">
        <location>
            <begin position="273"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Fascin-like" evidence="7">
        <location>
            <begin position="428"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013918144.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="54710" checksum="B0E028C00AC825B2" modified="2020-10-07" version="1">MTYSLEFGLVNAANRYLTAEPFRFQVTATGKQLKPRQVWVLQFVTPHGAGAAGPGEGAAQKVHLISALKRYLAADPNGKVTCDQDKPGPQTFFLLHQYPDGKVCLQNEQSKRYLGGAEDNVTCFAQASSETEKWTLHLAVHPNVALYNPNRKRYVRYDEKAEVLRCDRDVPWGAESVISIYYDFKVKKYGLRNGAGKLLASNGSLLSEVTAQTMYSLEVHGGLVAFRDDEGKYLTGREGSMKTLKMDRPGRDELFSVEASPAQVSLRSSSTNKFVCCKPGADLYATAMAVSNTEIFQLMFDDTTKKVCFRGHSGTYMSLGPNDCIISSTKQDKNAWFELQYQDQKVLLHMGDKYVSMRPNGQLLAVPKAAGKSEDFLIVLVNRPLLILHSDAGYVGLTSDMKRLEGNCSDYVLNSLSLSEDGYYNFQIGGKYWSLDKDGQILVTSDRPGNFSLQFVSSTSLIIKASNNKYLMADQGGRVWTGSTDASSATLFHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQL4</accession>
    <name>A0A6I9XQL4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock 70 kDa protein 12A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HSPA12A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with SORL1 (via cytosolic C-terminus); this interaction affects SORL1 internalization and subcellular localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912998.1">
        <property type="nucleotide sequence ID" value="XM_014057523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQL4"/>
    <dbReference type="GeneID" id="106541941"/>
    <dbReference type="KEGG" id="tsr:106541941"/>
    <dbReference type="CTD" id="259217"/>
    <dbReference type="OrthoDB" id="2963168at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11735">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000061">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000011">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026685">
        <property type="entry name" value="HSPA12A_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF46">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912998.1"/>
        </source>
    </evidence>
    <sequence length="670" mass="74656" checksum="0B243AE662832316" modified="2020-10-07" version="1">MAGTEGISADAVSTSAYASPARSLGDPGITPLSPSHIAKDSDANDAEEQTFLVVVAIDFGTTSSGYAYSFTKEPECIHVMRRWEGGDPGVANQKTPTTILLTPERKFHSFGYAARDFYHDLDPNESKHWLYFEKFKMKLHTTSNLTLETDLSAANGKKVKALEIFAYALQYFKEQALKELSDQAGSEFENSDVRWVITVPAIWKQPAKQFMREAAYKAGMASPEISEQLIIALEPEAASIYCRKLRLHQMIDLSSRAPVNGYTPSETIASGFTPAKEHIRRNRQSRTFLVENVIGEIWSELEEGDRYIVVDGGGGTVDVTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDHIIEHLSEIFDKPELTNVKFLFLVGGFAEAPLLQQAVQNSFGSKCRIIIPQDVGLTILKGAVLFGLDPAVIKVRRSPLTYGVGVLNRYVEGKHPTEKLLLKDGTRWCTDVFDKFISADQSVALGETVSRSYTPAKPSQLVIVINIYSSEHDNVNFITEPGVRKCGTLRLDLTGSDKSVPGRREIKTLMQFGDTEIKAMAIDVATSKSVKAGIDFLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRM6</accession>
    <name>A0A6I9XRM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquitin domain-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">UBTD2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918709.1">
        <property type="nucleotide sequence ID" value="XM_014063234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRM6"/>
    <dbReference type="GeneID" id="106546373"/>
    <dbReference type="KEGG" id="tsr:106546373"/>
    <dbReference type="CTD" id="92181"/>
    <dbReference type="OrthoDB" id="1640476at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd17121">
        <property type="entry name" value="Ubl_UBTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.225.20">
        <property type="entry name" value="Ub domain-containing protein, DC-UbP/UBTD2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032752">
        <property type="entry name" value="DC-UbP/UBTD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038169">
        <property type="entry name" value="DC-UbP/UBTD2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039869">
        <property type="entry name" value="UBTD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13609">
        <property type="entry name" value="UBIQUITIN DOMAIN CONTAINING 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16455">
        <property type="entry name" value="UBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="152"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918709.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="26407" checksum="41A048B5AED3E12B" modified="2020-10-07" version="1">MGGCVGSHHDSSGSLNENSDGTGVALGRNQPLKKEKPKWKSDYPMTDGQLRSKRDEFWDTAPAFEGRKEIWDALKAASHAFENNDHELAQAIIDGANITLPHGALTECYDELGNRYQLPVYCLAQPINMIEEKGDLETLDFPDPPPNSGHECQLRLRLSTGKDLKLVVRSMDTVYHMKRRLHTVEGVEPRSQRWFFSGRPLADKMKLEELKIPRDYVVQVIVSQPLTNPVLVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRQ1</accession>
    <name>A0A6I9XRQ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc finger CCHC domain-containing protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ZCCHC14</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913378.1">
        <property type="nucleotide sequence ID" value="XM_014057903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRQ1"/>
    <dbReference type="GeneID" id="106542238"/>
    <dbReference type="KEGG" id="tsr:106542238"/>
    <dbReference type="CTD" id="23174"/>
    <dbReference type="OrthoDB" id="6361509at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09558">
        <property type="entry name" value="SAM_ZCCH14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058599">
        <property type="entry name" value="PHAT_Smg/ZCCHC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057327">
        <property type="entry name" value="Vts1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037632">
        <property type="entry name" value="ZCCH14_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042344">
        <property type="entry name" value="ZCCHC14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16195">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16195:SF16">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26034">
        <property type="entry name" value="PHAT_SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25479">
        <property type="entry name" value="Vts1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="1045"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="420"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="503"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="558"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="896"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="503"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013913378.1"/>
        </source>
    </evidence>
    <sequence length="1086" mass="116764" checksum="811CD12C379712EE" modified="2020-10-07" version="1">MVEKRCPRLQRESVYRWFSELPSPQRVEFLCGLLDLCIPLELRFLGACLEDLARKDYHSLRDSEIKANNAADLGGLTNLTDEVVRSKLLVSLALLGSSHREAAGVLFRTLTHLDSVIHNYGLQLNEGRTGDEFLLLFTMASNHPAFSFHQKQVLRQELTKIQSLLACTAKGPPAAASAAATTAGAAATTCPGCHKVTPRSETPINHVVGNSLENALHTSAHSIEESLPKRPSGKHSKGTSQGFAEYSYLCVCVWRGRTFSVSYEDHSRGHVLWSDSSVTVVKKSSAEVTDFISKLGQLCPEENLEKFIPFLAGPESFYLERSHMDLESDLRYLASLPSHIWKNDHVKKFFSTSCHSPQYLSSSPGNSSLYKVGTTLGASGRPICGVAGVQAAQNTLQHHLPHPSAAAVALSHCSHSGGAGPTLACSRSQRENPSPVLMSPSPQNPQAQEQNGILDWLRKLRLHKYYPVFKQLTMEKFLSLTEEDLNKFETLTMGAKKKLKTQLELEKEKSEKRTMNPAPSPPVSSGVARVPPTTHINPIQSIRGNHATELHVDVDQAVSTTPPREGSSSESSSSSSSPMGMQPREESSDSAEENERRVEIHPEPSEKEKPVMILNHFGSSSARPTAQVLPVQNESGSSPAAHHTLPGQMTATPIRILNSMHKPERGNADLKLLSSPMHSLLPLEERTKFPGGPAQNSVKMEKSFGNTLADRMPVPPHQPLQVLSAVPENGPSTPSIHFGLRSKLVHAPALDRMAKPPQPPGILVETSTTATVTSSTVFHMARPPIKLLVSSSVPTDATLAGQPSCSNSMQFSVSPAIISPRTALYTANTKVAFSAMSGMPVAPMPSSTFCANSNPAASSSSHLATSFANMGSLPSCPPPSSSPTLSSAAENSFYGGGGSAGNMPVPNQNNHHHHHHHHQQPPGCMVCSSCGCSGNCGSSGMTVSYTNYFQHPFSGPSMFTFLPFNPLCSNGYVNPQQYNSSSAFPMVHSPYSGGPTPDSLMSGQSAFAVPPMQSFMAGTAGVYQAQGMVNSSGGSGHKKPGNLSCYNCGASGHRAQDCKQPPMDFNRQGTFRLKYAPPAESLESTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XRU1</accession>
    <name>A0A6I9XRU1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 12-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106542270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913418.1">
        <property type="nucleotide sequence ID" value="XM_014057943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XRU1"/>
    <dbReference type="GeneID" id="106542270"/>
    <dbReference type="KEGG" id="tsr:106542270"/>
    <dbReference type="OrthoDB" id="20828at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF3">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913418.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18839" checksum="7BDE7416DB9E8673" modified="2020-10-07" version="1">MAAFGLLSYEQRPLKRSRLGPPDVYPQDPKQKEDELTAVNVKQGFSNQPAFSGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKLQINAKDNYWLVTARSQSAIHTWFADLAGNKPLTFLAKKVLGWLLHYLGEWEEGSYHSDMNLKCNINKISKAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS49</accession>
    <name>A0A6I9XS49_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein C3orf17 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106546528</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918889.1">
        <property type="nucleotide sequence ID" value="XM_014063414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XS49"/>
    <dbReference type="GeneID" id="106546528"/>
    <dbReference type="KEGG" id="tsr:106546528"/>
    <dbReference type="CTD" id="25871"/>
    <dbReference type="OrthoDB" id="9899341at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045747">
        <property type="term" value="P:positive regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052835">
        <property type="entry name" value="Nepro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027951">
        <property type="entry name" value="Nepro_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34761">
        <property type="entry name" value="NUCLEOLUS AND NEURAL PROGENITOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34761:SF1">
        <property type="entry name" value="NUCLEOLUS AND NEURAL PROGENITOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14780">
        <property type="entry name" value="NEPRO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolus and neural progenitor protein-like N-terminal" evidence="3">
        <location>
            <begin position="11"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013918889.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="34517" checksum="2DE50FF84359D8DA" modified="2020-10-07" version="1">MAAAGAGEFVWNRWDVPWPACNSAEVVPAEHASVRCLSTALKKCGAVDSMLNSKMLVAEQSLLHSIIYNYHYQMIHQKAYGSLKQVEQCLKRFSLMNLEKSIQKIAQTGLIEQKSENSEESLVPSQPVIEVVLVKILGGCKLILRLLECCCTAFSLIVKHLCLEEYILFNTLILGLLSRLWILYGAVLKKLSTLYKDLFELLQEVAKMHQRPYIKGFVFPSEINDFLETSYLEIKKKMSKSFIERKAGTGWINTLFSVPKTISHSQNLSVAIPPAVRKKKKKKRKGTQNSSDIGKPVLINRTNQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUA2</accession>
    <name>A0A6I9XUA2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Major facilitator superfamily domain-containing protein 9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MFSD9</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914263.1">
        <property type="nucleotide sequence ID" value="XM_014058788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUA2"/>
    <dbReference type="GeneID" id="106542920"/>
    <dbReference type="KEGG" id="tsr:106542920"/>
    <dbReference type="CTD" id="84804"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17390">
        <property type="entry name" value="MFS_MFSD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF14">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914263.1"/>
        </source>
    </evidence>
    <sequence length="446" mass="49478" checksum="06477CF1875A9C74" modified="2020-10-07" version="1">MPINGLLLANINILHLIFLKKNHCLFSGFLWCQYDCPLVKPSCEIFRSEFNNCRSGSLYGILQLFSSTLVGSWSDLVGRPHCLLVCILFSSLGYFVLSISTNVFLFAIARTFVGIFKHTHSVSKVLISDMVPKKERLLVIGYFNAISNFGFILGPVVGGYLTELEGGFYLTAFICASIFVLNAVVFWMLLRYAKKMHSREWAILNKARSFIPEEVKHGLQTATSVHKTISSAQPKNSSCIQVTSVLKRLGSVANSNLWDVFLVRLLMSVAVMLYHSNFVLGLEERFGMKPKLTGYIISYSSALGVAAGFLLGPITKYYRHNTFAMLLHSTLLTCLFMFLYSVSESLWTLILSCTFLAFSTTVGRTCITELELMLGGDQASGMLIGVGQSVSAVGRIISPIISGVVQEYSPRGPPILGSLLGILAVLIMVFNRSRYSVRRTMKFKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUR7</accession>
    <name>A0A6I9XUR7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Group 3 secretory phospholipase A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PLA2G3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919854.1">
        <property type="nucleotide sequence ID" value="XM_014064379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUR7"/>
    <dbReference type="GeneID" id="106547269"/>
    <dbReference type="KEGG" id="tsr:106547269"/>
    <dbReference type="CTD" id="50487"/>
    <dbReference type="OrthoDB" id="10059604at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05826">
        <property type="entry name" value="Phospholip_A2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Phospholipase A2-like central" evidence="4">
        <location>
            <begin position="147"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013919854.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="32643" checksum="5953DD5A880E5F2C" modified="2020-10-07" version="1">MMPLAHLVEPSPYQPIEIPEATTFPLQPRQHRKPGLKGRKTRRKKVKKPSTETQDTVIPLASISKTNEATNLPGRSSAGEETTALQTTLNPGPIKREGEILTARHPVVLTVEEAFSNETWQGERPISGPSAGHSQVSKPRPGLSQRCRCYQRLDHCPFQIGPNEFKYQLHNSDDRTLYHCNCTRRLARFLRRRKSPNEVEEQVLSHYIAPSCFVLENLPGCRMGGESWPNCIGIGKAILAPARHLTNRLMGKRARGSLKVKRQEGAFLEGPARLFDKCVRLVRAALQPAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XV66</accession>
    <name>A0A6I9XV66_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Poly(RC)-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PCBP3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013920019.1">
        <property type="nucleotide sequence ID" value="XM_014064544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XV66"/>
    <dbReference type="GeneID" id="106547398"/>
    <dbReference type="KEGG" id="tsr:106547398"/>
    <dbReference type="CTD" id="54039"/>
    <dbReference type="OrthoDB" id="442947at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22519">
        <property type="entry name" value="KH-I_PCBP3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22522">
        <property type="entry name" value="KH-I_PCBP3_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000002">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000005">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="12"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="77"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="220"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013920019.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="31577" checksum="3FC15F84926EE839" modified="2020-10-07" version="1">MESKVSEGGLNVTLTIRLLMHGKSGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSTATSKPPVTLRLVVPASQCGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLESPPKGATIPYRPKPASTPVIFAGGQAYTIQGQYAIPHPDLTKLHQLAMQQTPFTPLGQTTPAFPGLDANPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANISLAQYLINARLTSEVTGMGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVU7</accession>
    <name>A0A6I9XVU7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chromosome alignment-maintaining phosphoprotein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CHAMP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918242.1">
        <property type="nucleotide sequence ID" value="XM_014062767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVU7"/>
    <dbReference type="GeneID" id="106546009"/>
    <dbReference type="KEGG" id="tsr:106546009"/>
    <dbReference type="CTD" id="283489"/>
    <dbReference type="OrthoDB" id="8016097at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039330">
        <property type="entry name" value="CAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37354">
        <property type="entry name" value="CHROMOSOME ALIGNMENT-MAINTAINING PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37354:SF1">
        <property type="entry name" value="CHROMOSOME ALIGNMENT-MAINTAINING PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="918"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="245"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="480"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="603"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="736"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="975"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="703"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="757"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="806"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="832"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="985"/>
            <end position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013918242.1"/>
        </source>
    </evidence>
    <sequence length="1016" mass="113804" checksum="B72CEC4A6FFC00FD" modified="2020-10-07" version="1">MCSVMQADSRKMDMLKILHKTTERLECDHCNFRGSDYENIQIHMGTIHPEFCDEMDTAGLGKLIFYQKSAKLFHCHKCFFTSKMYCNVYYHITAQHSLSDKQKENQKGVLETESLKKPIFVEPQKPSQSPETQKRTVSPDSQKPAPPELPKSDVGLHSKIQKPTSSTTIEHQKFVSVACLEAQKPAPVVPELPNPVSLVSSETLKPPLSVTPEPLKPASVDIAEIPKSLPALLLEQQKPVTPTYIETQKSLPVVESEKSASFISSEPQKPIPPVSPEAQKSAQPASPPVVMSETQKFAPAVSPEPRRHSPNVSPEPRKHSPAVSPEPRRHSPAVSPELKKPIAGLSTDARRHISQMRRPPSALSPEPRKPDPPLSPDPWRPVPETWRSAGSISHDQQKSITISPWSPKPQTSISLEPRKPASESRRPGNMMSSESQKFMPDTWKSTPMPESPKSSLASSEPWKPISSVYPEGWKPVLSPDTWRPSPSIAPPAPPPMPAELRKPGPPMSPDIWKQPFFSEQRKPTPSISSDPWKFSSESRKSPFFPEPQKPTTFISSELQKRALFPESRKRALFPESKKSVPPVSTDMQKRSFFHEAHIPFPETPKPVVFSESHRPTPVSPDVPKHSFFPDSQKHAVVSPEIQEHAPFSDSQKSPTLSHELLKSPAVSPEIQKHGTFAELQKPSPDSLEKQSFFAEPPKHSLSPEVQKSISSDQKPVQNTDTHKPVEAIGKEVHKTINDFSPPAAPVFSDLQTLNESSNKDFFTEHPEGENPFSSLLIPKQQPIQPKEPAEEIYTCPKKKAKKENPEISNTEFNSNESGNTEMETSDIKEQESNSDQEQFDTESPDQNKETKIDATPTPPQCVLQFTEEKEAFISEEEIAKYMKRGKGKYYCKICCCRAMKKGAVLHHLVNKHNVHSPYKCDVCGKAFLLESHLKNHVASHSQSLLKCPRCNFESNFPRGFKKHLTHCQSRHSEEVKKQLDIASEPPNQSQQNEEAGNVPEENAEPSKEFLNELQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWB4</accession>
    <name>A0A6I9XWB4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RWD domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RWDD1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Protects DRG2 from proteolytic degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RWDD1/GIR2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908840.1">
        <property type="nucleotide sequence ID" value="XM_014053365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWB4"/>
    <dbReference type="GeneID" id="106538757"/>
    <dbReference type="KEGG" id="tsr:106538757"/>
    <dbReference type="CTD" id="51389"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23816">
        <property type="entry name" value="RWD_RWDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000064">
        <property type="entry name" value="RWD domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="8">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="198"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013908840.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="26612" checksum="19FE3D5CD4C901CC" modified="2020-10-07" version="1">MKNNFSYRFFFLTVLSEKPTSFTITVTSEAGENDETVQTTLKFTYSEKYPDEVPLYELLAHENLENNDIADLLKLLQEQSEENLGMVMIFTLVSAVQERLNEIVDLIKTRREAEKKQKEKEAEEAEKQCFHGTPVTIESFLRWKATFDLELLEIKRKKMKEEEQCGKNKLTGKQLFETDHNLDTSDIQFLEDGGNSVEVDESLFQDMDDLELEDDEDDPDYSPVHLDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXC9</accession>
    <name>A0A6I9XXC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein KIAA0754-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920874.1">
        <property type="nucleotide sequence ID" value="XM_014065399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXC9"/>
    <dbReference type="GeneID" id="106548083"/>
    <dbReference type="KEGG" id="tsr:106548083"/>
    <dbReference type="OrthoDB" id="7301963at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026946026" evidence="2">
        <location>
            <begin position="21"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013920874.1"/>
        </source>
    </evidence>
    <sequence length="218" mass="22920" checksum="64C906C667976063" modified="2020-10-07" version="1">MKSIEIICCLFVVIFALAVAHPLPDNVLAQLEAKEDPTAPELPLKDPSAPELPLKDPSRPALLLKEPTAPALALKDPSRPALLLKEPTAPALALKEPSRPALLLKDPSAPALLLKEPTAPALALKEPSRPALLLKEPSAPTLALKEPSAPTLKDATAPADPQTLNADDNASSSSSSSSMSQNDLQEQQVEAILFTALSTKAEVANDGPYITCGLQFPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYE3</accession>
    <name>A0A6I9XYE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PPP1R12C</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">PP1 comprises a catalytic subunit, and one or several targeting or regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921254.1">
        <property type="nucleotide sequence ID" value="XM_014065779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYE3"/>
    <dbReference type="GeneID" id="106548413"/>
    <dbReference type="KEGG" id="tsr:106548413"/>
    <dbReference type="CTD" id="54776"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019208">
        <property type="term" value="F:phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000004">
        <property type="entry name" value="Phosphatase 1 regulatory subunit 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000898">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017401">
        <property type="entry name" value="MYPT1/MYPT2/Mbs85"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051226">
        <property type="entry name" value="PP1_Regulatory_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031775">
        <property type="entry name" value="PRKG1_interact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24179">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24179:SF27">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 12C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15898">
        <property type="entry name" value="PRKG1_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038141">
        <property type="entry name" value="PP1_12ABC_vert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="206"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="cGMP-dependent protein kinase interacting" evidence="9">
        <location>
            <begin position="700"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="631"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="701"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="551"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013921254.1"/>
        </source>
    </evidence>
    <sequence length="799" mass="88423" checksum="87F91548A4DC8664" modified="2020-10-07" version="1">MGSGGRPRAVRFDAAAEFLACCAGAELEAAREMLGADAAAVNGTNADGISALHQACIDENMEVVEFLVENGADVNQADNEGWTPLHVAASCGYNEIAQYLLDHGANIAAVNSDGDVPLDIAEADSMEALLRAEIEKRGVDVEVAKREEEEMMLRDARHWLNDGKISDEPHPKTGATALHVAAAKGYVEVMRLLLQAGYDTNVQDKDGWAPLHAAAHWGVEEACRLLAEHFCDMEALNNVGQRPCDLADEDTLALLEDLQHKQDDLRSKKEAHRQAVIDTSLEQAPSYTSKHRRSSVCRMSSKEKISVQDQSKERKTLGTIAVGEEETSSASEGDETPESNEVKAVPPADSGAVNGVSVTPIKPLSPSPLGPKFIGGPSRTTEPSSLPAPATWRQSLRKTSSFGVLQDAKLDEGASKGGSGIKRSASSPRLDTEEKQDIQELLKKWSKNPIGPWLANRNTASEPAGDPNKEPRLARVIPTPTRRIFALPDTEPHRDPRRTSYQMPVRDEESESQRKARSRLMRQSRRSTQGVTLTDLKEAEKTMVWTSDSKTPSEQRNKEDEKRDKDGEQQSQETTDSARRFRLLSSDSSSVSTVNPLVVNSQLQKGTEVDKAGSELDTDLRNRLAIRDRRKGRRERRITGLGGNSEGEEDEDTEQPLNEDPSMHSSDHLNSLLPNRTAPPGGQRGNSTLSGCREEPDNYYKKLYEDLLIENEKLKEQLQEAQLQLTQIKLELERVTQRQERFAERPALLELERFERRALERKAAELEEELKALSDLKADNQRLKDENAALIRVISKLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XYH8</accession>
    <name>A0A6I9XYH8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FYN-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Adhesion and degranulation promoting adaptor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">FYB-120/130</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">FYN-T-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">SLAP-130</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">SLP-76-associated phosphoprotein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">FYB</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton. Modulates the expression of IL2. Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013918856.1">
        <property type="nucleotide sequence ID" value="XM_014063381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XYH8"/>
    <dbReference type="GeneID" id="106546500"/>
    <dbReference type="KEGG" id="tsr:106546500"/>
    <dbReference type="CTD" id="2533"/>
    <dbReference type="OrthoDB" id="9396701at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11867">
        <property type="entry name" value="hSH3_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000156">
        <property type="entry name" value="FYN-binding protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000133">
        <property type="entry name" value="FYN-binding protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043443">
        <property type="entry name" value="FYB1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035540">
        <property type="entry name" value="FYB_hSH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029294">
        <property type="entry name" value="hSH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16830:SF13">
        <property type="entry name" value="FYN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16830">
        <property type="entry name" value="SH2 CONTAINING ADAPTOR PRAM-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14603">
        <property type="entry name" value="hSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 18" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="507"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="749"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="48"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="308"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="574"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="466"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="597"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="652"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="690"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="724"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013918856.1"/>
        </source>
    </evidence>
    <sequence length="831" mass="92147" checksum="932DF1B448285BD2" modified="2020-10-07" version="1">MDGKGNVKSIMAKFNKNAAEDVRCHQVRTGGHPPMAKKAAFEKFAQLENAGLPPKPNSLHKPGSLKLPPGAKPLHDASGKDFKTPPLQSKVKMLAEAASREANEKTTYPKPLVPKISEGLREETKPVFPRIPEKKLLGSPPQKSEPKPLEQRLFKPEPRGSEAKPVFPTVAGVKEKFNSAAQENAKPPFPKPPVKQKPNQSFSQGEEVSNKSACFNQVSPSSSGLKSKVGSIRPHPTPPQDKSRNEAKPISPFPPLKHVNNENNLPQIPPKPAGQLNEEAKPKPIKNIFLQNRQEDFGSASGTIAAKLANSSQTVKAGPWANNTGKEEKDKDKNLPRRKTLPSTITLGPAPNKPSRPPTVDLEKFRKSHGEGSSSSNSVGLPPPLPPPIHAIQSTDISSPPPPPPPGFHPSQTPVLPPRGKIPSRPDVRGEENEENYDDIEFDTGNADDNSDGETYEDINEMRFTPQEEEKKKKEEKKKADQKKEQREKDKKEQELRKKFKLEGPIEVIHQARASTDCKGGKYDLSFKQGDLIEILRVTDNPEGKWLGRLKGSYGYIKTTMVAIDYDTLKKRPRPPVHVQAKQPDSDQEVYDDVGDQDSISSGSQSTMGGFPPPPSDDIYDGVEDDEIPAKSVSQDEEKSDTWSKGLLKILKGKDYQKKSMREAPSKVNVTEDENSLRSPLAKPTGTDTGDSDVYDDVESTDFQLPPKEISLGKNIKSLNFGRGKSDERDSQKFKKMDKEEKDFRKKFKYEGDIRVLYTTTISNAPSLKKRGSKDLQVKPGELVEVIQDVDETKVLCRNEEGKYGYVQRSCLVHDDEEIYDDIADDSYDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZG5</accession>
    <name>A0A6I9XZG5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Denticleless protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DTL</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the WD repeat cdt2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919301.1">
        <property type="nucleotide sequence ID" value="XM_014063826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZG5"/>
    <dbReference type="GeneID" id="106546854"/>
    <dbReference type="KEGG" id="tsr:106546854"/>
    <dbReference type="CTD" id="51514"/>
    <dbReference type="OrthoDB" id="2096344at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051247">
        <property type="term" value="P:positive regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000447">
        <property type="entry name" value="Denticleless protein homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000171">
        <property type="entry name" value="denticleless protein homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051865">
        <property type="entry name" value="WD-repeat_CDT2_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852:SF0">
        <property type="entry name" value="DENTICLELESS PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852">
        <property type="entry name" value="LETHAL 2 DENTICLELESS PROTEIN RETINOIC ACID-REGULATED NUCLEAR MATRIX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 16" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="92"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="217"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="354"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="655"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="541"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="661"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013919301.1"/>
        </source>
    </evidence>
    <sequence length="723" mass="80206" checksum="750F46235E722A94" modified="2020-10-07" version="1">RFITFVLHRQERKALSLAPRFSLHHLLGQYQCIKEDDHPSYGDMGIPVPPFGCSFSTAPDVQHVLGVANEEGFVRLYDTHTQLNNKKIVKEWQAHLNAVFDLAWMPYEHKIVTASGDQTAKVWDIKTGELLGVCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMIWDTRCSKKDGFYRQVKQISGAHNIIDKTPSKLRKKKQNVKGLAPSVDFQQSVTVVLLQDEHTLISAGAVDGVIKIWDLRKNYTAYRQEPVPSRVFSYPGNSTRKLGYSSLILDSTGTNLFANCTDDNIYMFCMPGLKNNPVSVFSGHQNSSFYVKSSISPDNQFLISGSSDQCAYIWKVSESQFPPMVLHGHLQEVTSVAWCPSDFTKISTCSDDNTIKIWRLRQSTKEKTWTDKTSLVGWTDQKTVVEQNKIEVLSSRHGTPPKVLPTGSPLISSPQPAACAPTYTGDLPLPTNTPTSSSATTPSISICTFTPAKQKVTSSTLSKLVLPSTTSIQHWVTKTPNSSPPVRETKIASPQIAAADIPDTAFASTSPHLRSETRAKRRLESSREENMCLKSCNCMTEHHPESKKSRQEQFTLAASSKPCRGDCLTLNGEVQNIQNLKEPFSPSSPCSSATCLNYPHLIETSCEKMVDKENGSTDRRTWLSDLGNKLRTNKMNNSSGSSSSLRNPSTKRVEARTGATSPGPVNYDYNNNEEDLYILRAKVTKKLKNYVLSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZQ9</accession>
    <name>A0A6I9XZQ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ryanodine receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Skeletal muscle calcium release channel</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Skeletal muscle ryanodine receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Skeletal muscle-type ryanodine receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Type 1 ryanodine receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106547312</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homotetramer. Can also form heterotetramers with RYR2. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Repeated very high-level exercise decreases interaction with PDE4D and protein phosphatase 1 (PP1). Interacts with CALM; CALM with bound calcium inhibits the RYR1 channel activity. Interacts with S100A1. Interacts with FKBP1A; this stabilizes the closed conformation of the channel. Interacts with CACNA1S; interaction with CACNA1S is important for activation of the RYR1 channel. Interacts with CACNB1. Interacts with TRDN and ASPH; these interactions stimulate RYR1 channel activity. Interacts with SELENON. Interacts with scorpion calcins (AC P0DPT1; AC P0DM30; AC A0A1L4BJ42; AC P59868; AC P60254; AC B8QG00; AC L0GBR1; AC P60252; AC P60253).</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919912.1">
        <property type="nucleotide sequence ID" value="XM_014064437.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106547312"/>
    <dbReference type="KEGG" id="tsr:106547312"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005790">
        <property type="term" value="C:smooth endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005219">
        <property type="term" value="F:ryanodine-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014808">
        <property type="term" value="P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006941">
        <property type="term" value="P:striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12877">
        <property type="entry name" value="SPRY1_RyR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000009">
        <property type="entry name" value="Ryanodine receptor 1 (skeletal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000002">
        <property type="entry name" value="ryanodine receptor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013333">
        <property type="entry name" value="Ryan_recept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035761">
        <property type="entry name" value="SPRY1_RyR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399">
        <property type="entry name" value="B30.2/SPRY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46399:SF10">
        <property type="entry name" value="RYANODINE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00795">
        <property type="entry name" value="RYANODINER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0702">S-nitrosylation</keyword>
    <feature type="domain" description="MIR" evidence="14">
        <location>
            <begin position="49"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="14">
        <location>
            <begin position="100"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="14">
        <location>
            <begin position="161"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="13">
        <location>
            <begin position="380"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013919912.1"/>
        </source>
    </evidence>
    <sequence length="691" mass="76659" checksum="D30A1D4512D9B925" modified="2020-10-07" version="1">MYLSCLTTSRSLTDKLAFDVGLQEDASATYPGEACWWTIHPASKQRSEGEKVRVGDDLILVSVSSERYLHLSTASGELQADASFMQTLWNMNPISSGCEEGCVTGGHVMRLFHGHMDECLTISTTDQNEEQRRVVNYEGGPVCSQARSLWRLEPLRISWSGSHMKWGQPFRVRHVTTGRYLALTEEKGLVVVDAEKANTKATAFCFRVSKEKLDVAPKRDVEGMGAPEIKYGESMCFVQHVDSGLWVTYAAADAKAMRLGLLKRRGWVGVSPLFQGMITLVLNCIDRLNVYSTAAHFAEFAGEDAAESWKEIVNLLYELLASLIRGNRSNCALFSNNLDWLVSKLDRLEASSGILEVLYCVLIESPEVLNIIQENHIKSIISLLDKHGRNHKVGCGELDSSLRFSSGGGGRLDFVASNVGYRRXKLVAXGLQLPEFVSHMRPNIFLGTHEGSTQYKKWYYEMIVDSVEPFVTAQATHMRVGWAMAEGYSPYPGGGEGWGGNGVGDDLYSFGFDGLHLWSGRVARAVASPGQHLLGADDVVSCCLDLSVPSISFRINGFPVQGMFENFNLDGLFFPVISFSAGIKVRFLLGGRHGEFKFMPPPGYAPCYEGVLPREKLRVEPIKEYKHDINGIRNLLGPTQSLSHTSFTPYPVDTVQAAITALYTELVMDPNTFQLCYSDQRILCISRIHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZS7</accession>
    <name>A0A6I9XZS7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106544396</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013916128.1">
        <property type="nucleotide sequence ID" value="XM_014060653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZS7"/>
    <dbReference type="GeneID" id="106544396"/>
    <dbReference type="KEGG" id="tsr:106544396"/>
    <dbReference type="OrthoDB" id="18798at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04146">
        <property type="entry name" value="RERG_RasL11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916128.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="27239" checksum="9CA776A3A0D6934A" modified="2020-10-07" version="1">MSQHSMNFLLVPIPEYPLLECAPNKVLKLVVLGGSGVGKTALVVRFLTKRFIGDYEANTGALYSRKFTIDGEQISLQVQDTPFVSLEDENDAICCQEQINRSIYWADGFVFVYSITDYESYRVIRPLHQHIRKIHPNANIPLLLMANKGDLLRARQVSTKEGHQLANELGCPYSEVSARENCEGVHGAFQQLCQELSRIIGNCNGEKRRGLHLIRPKSPNMQELKRRLKQALSSKGKTATVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZT1</accession>
    <name>A0A6I9XZT1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Neuronal acetylcholine receptor subunit beta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CHRNB2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919461.1">
        <property type="nucleotide sequence ID" value="XM_014063986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZT1"/>
    <dbReference type="GeneID" id="106546971"/>
    <dbReference type="KEGG" id="tsr:106546971"/>
    <dbReference type="CTD" id="1141"/>
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        <property type="entry name" value="NRIONCHANNEL"/>
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    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
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            <begin position="134"/>
            <end position="381"/>
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        <location>
            <position position="1"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
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        <source>
            <dbReference type="RefSeq" id="XP_013919461.1"/>
        </source>
    </evidence>
    <sequence length="400" mass="45508" checksum="5FA35DFFC365EFDC" modified="2020-10-07" version="1">PSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSDVASLDDFTPSGEWDIMALPGRRNENPNDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMPPWVKVVFLEKLPTLLFLKQPRQSCARQQLRQKRQSQERALGSFFMPRGTRSCTCYVNPSTVKKFGTGSSSGRTFTESSEGVNGFRERLGQAAPEQEACCCGLEEAIDGVRFIADHMKSEDNDQSVSEDWKYVAMVIDRLFLWIFIFVCVFGTIGMFLQPLFQNYATNSLLQINHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0M3</accession>
    <name>A0A6I9Y0M3_9SAUR</name>
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        <submittedName>
            <fullName evidence="14">Dyslexia-associated protein KIAA0319-like protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KIAA0319L</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014054815.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="4"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000258">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Cadherin-like_dom"/>
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    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029865">
        <property type="entry name" value="KIAA0319-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056502">
        <property type="entry name" value="KIAA0319-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013980">
        <property type="entry name" value="MANSC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46182:SF3">
        <property type="entry name" value="DYSLEXIA-ASSOCIATED PROTEIN KIAA0319-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46182">
        <property type="entry name" value="FI19480P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22352">
        <property type="entry name" value="K319L-like_PKD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23620">
        <property type="entry name" value="KIAA0319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23597">
        <property type="entry name" value="KIAA0319_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="997"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="536"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="11">
        <location>
            <begin position="585"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="196"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1049"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1053"/>
            <end position="1069"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013910290.1"/>
        </source>
    </evidence>
    <sequence length="1116" mass="122445" checksum="ECFCA9701BCFA93E" modified="2020-10-07" version="1">MEKSLESSFSINFLNPLVFFFRKSAKCLGHLPLIYIFICLSALSFCTDGSWSRSACKSGKPLEEGNLRSSDSVQLQLLEGLHSIHSCQAACCQTPACDAFWLMGTLCIQVNCTNSHKCQEIRTDMVDPIMVFVTKSDLDDVDSQPAKERQAHLPKWLNWKERHQSKSRIQRSFKELPAHTLHLGNVRGISLKRDVDSSVGSSINHPAPSEALDRIPTHKDLTEPQSSRPEANLSGNHTKVEKILLTNDKDSKELKPQNPSSNSDPKNREHGLGGSGSLGAPTGVSTPYPSSATIFPSPVGNSEKTRPSNNVSIQTFSLEKAIPTSDSLVGKDLGEVPQVIPVPTGIPINGSVTAVLSDPAIVPSTPAPTPVIKKLTVSAGDSVQVTLPKNEVELNAYALPSSPKGSSYSYDWRLITHPKDYSGEMEGQHSQILKLSKLTVGLYEFNVIVDGDNAHGEGMVNVTVKPEPRVNQPPVAIVSPPFLEISLPTTSTVIDGSQSTDDDKIVSYHWEELKGPLREEKLPADTDILELTNLVPGNYTFRLTVVDSDGATNSTTASLTVNKAVDYPPVANAGPNQVITLPQNSITLYGNQSTDDHGIISYEWSLSPNSKVKVAEMQGVRTSTLQLSAMREGDYTYQLTVTDSAGHQSTAEVTVIVQPENNKPPRADAGPDKELILPVDSTTLDGSKSSDDQKIASFLWEKTQGPDGVKLENANSSIATVSGLKVGVYKFTLTVKDERNLQGQSSVTVIVKEEMNQPPVAKIMGNTVTVTLPTNTAELDGSQSTDDKGIVTFLWTRDESSPAAGEVLNNSDHHPILFLSNLVEGTYTFHLKVIDAKGESDVDRATVEVKADPRKNNLVEMILDVNVSHLTERQKGMFIRQIGVLLGVLDSDITVQKIQAYTEKSTKLVFYVQNQPPHQIFKGRDIAWTLKSELRKQQSDFLIFRALEINTVTCQLNCSDHGLCDSFTKRCICDPFWMENFIKVQIGDGESNCEWSVLYVVIASFVILVSLGIFSWVVVCCCKKKKGKPKRKSKYKILDATDQESLELKPNSKAGSKQKSQAQNTSLMHSESELDSDEAIFTWPDREKGKLLHGQNGFLRNGQVMLKPKNQREEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2Z6</accession>
    <name>A0A6I9Y2Z6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-galactosidase</fullName>
            <ecNumber evidence="5">3.2.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Acid beta-galactosidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106540590</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. May form higher multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013911240.1">
        <property type="nucleotide sequence ID" value="XM_014055765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2Z6"/>
    <dbReference type="GeneID" id="106540590"/>
    <dbReference type="KEGG" id="tsr:106540590"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042340">
        <property type="term" value="P:keratan sulfate proteoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000115">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000017">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026734944" description="Beta-galactosidase" evidence="15">
        <location>
            <begin position="16"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="16">
        <location>
            <begin position="33"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013911240.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="49530" checksum="906BE7C3A6A988F9" modified="2020-10-07" version="1">MLSLVVLHFLAAGFGSPVTHQRNFEIDYARNCFLKDGKPFRYISGSIHYARIPRFYWKDRLLKMKMAGLDAIQMYVPWNFHEPEPGVYNFRDDRDLEYFLKLADEIGLLVILRAGPYICAEWDMGGLPAWLLEKDSIVLRSSDSDYLAAVGRWMRVLLPKMKPHLYQNGGPIIMVQVENEYGSYFACDFHYLRYLKNLFRQYLGNEVVLFTTDGATKIFLRCGTLQGLYSTVDFGPHTNVTAAFLTQRRTEPQGPLVNSEFYTGWLDHWGHPHNVIPSPAIAKTLSEILAHGANVNLYMFIGGTNFGYWNGANIPYMAQPTSYDYDAPLSEAGDLTEKYFALREVIGMYKDLPKGPIPPTTPKFAYGEVKLMKVGTVTELLNEFSPSGPIKSMYPLTFVQLKQVKVDSYIDFLTHNLASLFNYVHHIKIFIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y335</accession>
    <name>A0A6I9Y335_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger protein ZPR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ZPR1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911295.1">
        <property type="nucleotide sequence ID" value="XM_014055820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y335"/>
    <dbReference type="GeneID" id="106540636"/>
    <dbReference type="KEGG" id="tsr:106540636"/>
    <dbReference type="CTD" id="8882"/>
    <dbReference type="OrthoDB" id="308464at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000003">
        <property type="entry name" value="zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="6">
        <location>
            <begin position="93"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013911295.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="32444" checksum="A7B874A0CD5C8296" modified="2020-10-07" version="1">MDTEVAGKIDEFISKLKGLKEAESAFTFILDDPSGNSFVENPHAPQKDEALEVTHYRRTAEQAALLGLEEEGLEEDPADPAEDLRNEVLQFNTNCPECNAPASTNMKLVQIPHFKEVIIMATNCDACGHRTNEVKSGGGIEPLGTKITLHVTGSADMMRDVLKSETCRIEIPELEFEIGMGALGGKFTTLEGLMKDIQALVEKNPFTLGDASCPGKAAKLHAFGKKLQQIVDGEMKVHLVLDDPAGNSYLQNLYAPEEDPELCVERYERRFEQNEELGLNDMKTEGYQADASSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3C8</accession>
    <name>A0A6I9Y3C8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neurensin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NRSN1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917768.1">
        <property type="nucleotide sequence ID" value="XM_014062293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3C8"/>
    <dbReference type="GeneID" id="106545661"/>
    <dbReference type="KEGG" id="tsr:106545661"/>
    <dbReference type="CTD" id="140767"/>
    <dbReference type="OrthoDB" id="5979667at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024883">
        <property type="entry name" value="Neurensin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14796">
        <property type="entry name" value="NEURENSIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14796:SF6">
        <property type="entry name" value="NEURENSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14927">
        <property type="entry name" value="Neurensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013917768.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15030" checksum="6D094C11219DCCFB" modified="2020-10-07" version="1">NLSVFQVGLISGTMFMLTGIIVLVVGFLVTPKIEALGVEDFVVVDTHAVQFNRALDVCKLAGAILFCIGGTTMAACLLMSAFAKSYSKEEKYLQQKFKERIADMKVQTYPVTKAPAAPGESKIPVTLSKVQNIQPLSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y434</accession>
    <name>A0A6I9Y434_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106546201</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918468.1">
        <property type="nucleotide sequence ID" value="XM_014062993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y434"/>
    <dbReference type="GeneID" id="106546201"/>
    <dbReference type="KEGG" id="tsr:106546201"/>
    <dbReference type="OrthoDB" id="9829410at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="79"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="160"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="208"/>
            <end position="227"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="239"/>
            <end position="260"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="42"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013918468.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="35306" checksum="18055949E03D8E92" modified="2020-10-07" version="1">MEAEKNRTLLTDFVLAGFTTDPFLRIIFFILFLAAYFITLTGNLGLIALIYLDSRLHTPMYFFVGNLSFLDIWYSSVYTPRILFDCVSKNNHISLAGCAAQFFFSAGLAYTECFLLAFMAYDRFVAICNPLLYAPAMSRDFCTRLVVGAYVAGFASAIILTGNTFHLRFCGNGVINHYFCDVAPLVKMACDDIGIYDLFVSSLMGCNLLATTAVILRSYIGIGAAIVRIRSAEGRRKAFFTCSAHLISVTLFYGSILIMYSRPNSQHTPKWDKANSLFYTVVNPLVNPFIYSLRNKDVKAAFKNVWGKFTLSFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4Q5</accession>
    <name>A0A6I9Y4Q5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Microtubule-associated serine/threonine-protein kinase 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106546710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919128.1">
        <property type="nucleotide sequence ID" value="XM_014063653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4Q5"/>
    <dbReference type="GeneID" id="106546710"/>
    <dbReference type="KEGG" id="tsr:106546710"/>
    <dbReference type="OrthoDB" id="9946270at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919128.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="29342" checksum="A31F0BA406CF53F8" modified="2020-10-07" version="1">MGKKVSAAAAAAKEEAERRDEGGQRLSPSSSSSSSSSSGSSPSSSDSQTLSEEGEAGSATPPASRGAFQRADPHRRDGTSEPQRPGGEAPGGPATLPSVAPRAGKEGGEVEKEGCVDGRQESEEEEDDSGSPGGLDGCGGARPRPETDASLEEQDEELDSILSPPPMPFRKSSNPEVSSGPGKSLKFKRQLSEDGRPFRRGSLGGALTGRYLLPNAVTQHSWQTGETSNLVRMRTQALGQSAPSLTASLEGIPPKSHFSSIRHRQQLVEPAATKNSNGNLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y521</accession>
    <name>A0A6I9Y521_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GRB2-associated and regulator of MAPK protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GAREML</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAREM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912765.1">
        <property type="nucleotide sequence ID" value="XM_014057290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y521"/>
    <dbReference type="GeneID" id="106541760"/>
    <dbReference type="KEGG" id="tsr:106541760"/>
    <dbReference type="CTD" id="150946"/>
    <dbReference type="OrthoDB" id="6077228at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd09525">
        <property type="entry name" value="SAM_GAREM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025946">
        <property type="entry name" value="CABIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052281">
        <property type="entry name" value="GAREM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454:SF5">
        <property type="entry name" value="GRB2-ASSOCIATED AND REGULATOR OF MAPK PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454">
        <property type="entry name" value="GRB2-ASSOCIATED AND REGULATOR OF MAPK PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12736">
        <property type="entry name" value="CABIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CABIT" evidence="4">
        <location>
            <begin position="30"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="497"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="620"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="731"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="646"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013912765.1"/>
        </source>
    </evidence>
    <sequence length="879" mass="96231" checksum="9B237E0948F79E2E" modified="2020-10-07" version="1">MERLAAALGRLAWSSGPPLPLDLIVAKCRLPALVRPGPGEYVEGVSDQDVLLIHSCRQWTTVTAHSLEEGHYVIGPKIDIPLQYPGKFKLLDQDRDVREPVQYFNSVEEVASIFPDRVFVMEAITFSVKVVSGEFSEDSEVYNFTLHAGDELTLMGQAEILCAKAAKEKSRLNTLLRKLGKAGVPGTGGGNGGSGNSSGSNNPGGGSGKQLKGKMPCLICMNHRTNESLSLPFQCKGRFSTRSPLELQMQEGEHTIRSIIEKVRLPVNVIVPSRPPRNPYDLHAIREGHCYKLVSIVSKTVVLCCILRKDEVAPFHFLLLTDMPRFLLPEGMLRVGGDPQLEKLLRETAALCQECFDPNEYSKAVREAKPDFSEECASPRHVRLCLQGYSRDELAQSFQRLSLCLYATSESSCQDPSQGGRTQRLLLPSHREPSSLISDPLDSEREYMTPDWVEPTFRTQELPYEELWTNQNPETYSESSGTVPGLENASKGQRDLISFGASPCHRGLPQEDLNKDAPPPVPPKSEAVREECRLLVAPPVPPRGAHTSATSSPPMPVRFPKLQAAVSPSANLSYYSSGLHDISRPRSGSCSPSPDSYSLYCYPCTWSDCKVGESTSRQLASPQAQLPSQPPPAQMSSWSDPWPCNDPSSSVATGRSTPLLGSTDANAVKSYYSCPRLKPPPPQKRFAPFGALNPFSNPAYSSSPASSGSSDWLESLDWQKTTASSIDTFDLFEGTSSPEGHCSPAPPPRSSKPFDTAENIVIRTSVAPLSSTIVHGSEGGITHLYLAQGVIEAPPARLNGDGSPWQPPGDLSALSLEEVSRSLRFIGLSEDVVSFFARERIDGSIFVQLTEEILSEDFHLTKLQVKKIMQFIKGWRPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y602</accession>
    <name>A0A6I9Y602_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Interferon tau-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106547559</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920253.1">
        <property type="nucleotide sequence ID" value="XM_014064778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y602"/>
    <dbReference type="GeneID" id="106547559"/>
    <dbReference type="KEGG" id="tsr:106547559"/>
    <dbReference type="OrthoDB" id="8922121at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF73">
        <property type="entry name" value="INTERFERON BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00266">
        <property type="entry name" value="INTERFERONAB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00076">
        <property type="entry name" value="IFabd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3 8" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027077191" evidence="9">
        <location>
            <begin position="22"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013920253.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="23508" checksum="6C7AE962096F3302" modified="2020-10-07" version="1">MIMNSCCLYLCIGIIFGEISCENCNQLQRKLLKANQVNFKLLSSNIKSTIPLQCYSDITDLSVEGIEKILIDMNDESQVGIAKTTIEEILQQIDLIFRQNHPKSIWHGDSLRDFHIGLDLQIKMLETCGNAKMEQVITSPRNLKLQLPRLRVKRHFQKVRDFLKKKKYSLCAWKIVQIEMKQFLERINYYIQRIPSKAIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y687</accession>
    <name>A0A6I9Y687_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Leucine-rich repeat-containing protein 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LRRC4B</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913785.1">
        <property type="nucleotide sequence ID" value="XM_014058310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y687"/>
    <dbReference type="GeneID" id="106542528"/>
    <dbReference type="KEGG" id="tsr:106542528"/>
    <dbReference type="CTD" id="94030"/>
    <dbReference type="OrthoDB" id="28057at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099151">
        <property type="term" value="P:regulation of postsynaptic density assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905606">
        <property type="term" value="P:regulation of presynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000012">
        <property type="entry name" value="Leucine rich repeat containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026923432" evidence="16">
        <location>
            <begin position="38"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="351"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="462"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013913785.1"/>
        </source>
    </evidence>
    <sequence length="646" mass="72410" checksum="C493E7AE6D398C16" modified="2020-10-07" version="1">MATRRCTRKPWLAPGISRMIWKHLVLISLWLPSFVLGGASPTSCPAACSCSNQASRVICTRRELVEVPESISINTRYLNLQENNIQVIKTDTFKHLRHLEILQLSKNLIRKIEVGAFNGLPNLNTLELFDNRLTTVPTQAFEYLSKLRELWLRNNPIESIPSYAFNRVPSLRRLDLGELKKLEYISEAAFEGLVNLRYLNLGMCNLKDIPNLTALVRLEELELSGNRLDMIRPGSFQGLTSLRKLWLMHAQVATIERNAFDDLKSLEELNLSHNNLMSLPHDLFTPLHRLERVHLNHNPWHCNCDVLWLSWWLKETVPNNTTCCARCHAPPNLKGRYIGELDQSHFTCYAPVIVEPPTDLNVTEGMAAELKCRTGTSMTSVNWLTPNGTLMTHGSYRVRISVLHDGTLNFTNVTVQDTGQYTCMVTNSAGNTTASATLNVSAVDPATTGYTYFTTVTVETMDSGQEESIPTKKEPGPTPSQGWGMTYSTTSLTPRSTRSTEKPFTIPITDVTENGMKDLDDVMKTTKIIIGCFVAITFMAAVMLIVFYKLRKQHQLHKHHGPTRTIEIINVEDELPAAAGAPGDSHLALPAIEHDHLNHYAAFKAHYNNNSGGGPLTCSKNPMLNSIHEPLLFKSSSKENVQETQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8V7</accession>
    <name>A0A6I9Y8V7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 64</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMEM64</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917595.1">
        <property type="nucleotide sequence ID" value="XM_014062120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8V7"/>
    <dbReference type="GeneID" id="106545522"/>
    <dbReference type="KEGG" id="tsr:106545522"/>
    <dbReference type="CTD" id="169200"/>
    <dbReference type="OrthoDB" id="166803at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045780">
        <property type="term" value="P:positive regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045672">
        <property type="term" value="P:positive regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053069">
        <property type="entry name" value="TVP38/TMEM64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46593">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46593:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="2">
        <location>
            <begin position="167"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013917595.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="39537" checksum="688F6727771E2AE6" modified="2020-10-07" version="1">MWSSGAAALQLLSRATKQALAQGARQDLNRWLQPLGAAGEPAEAASRGASGEPLLFGEQNGLYHCPPPHPSQEPSVELLLCQLPEAGHGLGESRTWHCSGCLLGTCWCKSCLSACFLAMFCFASLALMRQYLRDLMLWAESLDSLAGVLLFTVGFIVVSFPCGWGYILLNVAAGYLYGFVLGMGLMVLGVVIGTFISHVVCKKLLARWVLARIQGSEKLSAVIRVVEGGSGLKVVALARLTPIPFGLQNALFSITDLSLPNYLMASSIGLLPTQLLNSYLGTTLRTMEDVIAEQSFSGYFIFILQIVISIGLMFYVVHRAQMELNAAIVACEIEMKTSLVKSSQISSNNSTSCNKRTLAFSGGGINIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y8X8</accession>
    <name>A0A6I9Y8X8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCDC15</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917700.1">
        <property type="nucleotide sequence ID" value="XM_014062225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y8X8"/>
    <dbReference type="GeneID" id="106545594"/>
    <dbReference type="KEGG" id="tsr:106545594"/>
    <dbReference type="CTD" id="80071"/>
    <dbReference type="OrthoDB" id="10007210at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037693">
        <property type="entry name" value="CCDC15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14817">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14817:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013917700.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="55178" checksum="0154157693455570" modified="2020-10-07" version="1">MVPLRKGLQQQKIAQTNLRPPSINWNVLAGRTQCIAPVGAWVESAQDDGEESPVFATAIQTEKEYKEQQKEKERTLKHFQQEVKRRVNQCVNLKRKQQLKTSYEAALKESSVIIGFSDSALRLTPKKNTCLYRYTRDSAIGKSNDKVTYVQQADYMPEQFSELAKTVRKTVQQVRHKLASRKAVPEGNEPPEHSGGAHRNSPRRQNIEPYNTTTLSPEEGDMGELPLKGRHDLPAELQEQEGGNWDNIEFQKVNNGTLKDNNLADIEAHAVHIFWPGVEEEQSKKQHQSQYLRYRRLFMDIEREQVKEQQRQKEWQRKMDKIRKEKEYRRRAEEQRIQEAQRAKEKGTQEVASQKSAHPRGKTCEKLQQLKLEDPEEKKKQKSKEYSRYVEALRAQMREKIDMYNIHLPPLCFCGSDFWDTHPDSCANNCMFYKNHKAYAQALQNVISSCDILDGGSNTRLSVHTFAVAHARSKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9T9</accession>
    <name>A0A6I9Y9T9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein spinster homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SPNS2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919255.1">
        <property type="nucleotide sequence ID" value="XM_014063780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9T9"/>
    <dbReference type="GeneID" id="106546811"/>
    <dbReference type="KEGG" id="tsr:106546811"/>
    <dbReference type="CTD" id="124976"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046624">
        <property type="term" value="F:sphingolipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003376">
        <property type="term" value="P:sphingosine-1-phosphate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
        <property type="entry name" value="MFS_spinster_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF4">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE TRANSPORTER SPNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919255.1"/>
        </source>
    </evidence>
    <sequence length="398" mass="43281" checksum="30223D4683A6D679" modified="2020-10-07" version="1">MVAAPIFGYLGDRFNRKIILSCGIFFWSAITFSSSFITEQYFWLFVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQVAGDWHWALRVSPLLGMITGTLILIFVPAAKRGNAEQLGAQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGMWFPVYLHRAQVVQKTAEICTSQPCGTKDSLIFGAITCFTGFLGVITGAGATKWCRVKTQRADPLVCAVGMLGSAIFTCLIFVAAKNSIVGAYVCIFIGETLLFSNWAITADILMYVVIPTRRATAVALQSFTSHLLGDAGSPYLVGFISDVIRQSTKESPLWEFLSLGYALMLCPFVVVLGGMFFLATALFFLSDRAKAEQQVNQLMMPPTSVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9U2</accession>
    <name>A0A6I9Y9U2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106546823</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106546823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013919270.1">
        <property type="nucleotide sequence ID" value="XM_014063795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9U2"/>
    <dbReference type="GeneID" id="106546823"/>
    <dbReference type="KEGG" id="tsr:106546823"/>
    <dbReference type="OrthoDB" id="2428204at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061172">
        <property type="term" value="P:regulation of establishment of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17117">
        <property type="entry name" value="RA_ANKFN1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039269">
        <property type="entry name" value="ANKFN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437:SF2">
        <property type="entry name" value="ANKYRIN REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437">
        <property type="entry name" value="WIDE AWAKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="523"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919270.1"/>
        </source>
    </evidence>
    <sequence length="639" mass="72061" checksum="1E89079B704977C3" modified="2020-10-07" version="1">KQKEGDLSSKVQNFLTVQAEIFGLVKRDVDQLHQLSDGPLELLKKKLQNSRKSISSPEEPTALDVLLMTMQEKLAYHQNSSQALPPGLYLGYLKLCSAVDQIRVLVPEQLPNILCHVKIRSNSNISRVHTSSARSLEKVSNNAKDFRLYSQEVLDFGRQVSFLLLLPPSDDVCTAPGQNNPYTPRSGFLTLPLQIFELVHFFIYDHEFISQYCQVSAFLELESLLSQQSLREAFSDSELTTAKQRHQQVQDYIQQMEEIWREMRWMMDALQHARYKQPSCGVPLGWFVKESSTAQNEKTQSTSSHLDFLPSPTPSPDMNRKLNSDSHGISDEEGSSEVFLATDSDYDSSSAQSPRELDLVYSSSGLECYRRRTPRPFRDSAPDVLQNHELKMPPIMPPKEPQPLSEQYDSDFVLPSRQIELLRITEKRQAYCVRTSSLDFPKPFCFGARKSCPGSVDSSPTESRTAGHCSSVPSGTRSTFSSSHDWHAESSGAVFRTRSVEWTQTFQETSEPGHVANWGRLPGSFIVRVCAQYETGLSKETSVKLPLTNKMSAEEVVKLVVQETNEISRKVLGNASAVCYSEEQFGHFALVFVTEDVEQCLPDNFLPLSLQKAWPNGRFCVRIKDTSPLLFRYGPATTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB06</accession>
    <name>A0A6I9YB06_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">PAS domain-containing serine/threonine-protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">PASK</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013921289.1">
        <property type="nucleotide sequence ID" value="XM_014065814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB06"/>
    <dbReference type="GeneID" id="106548442"/>
    <dbReference type="KEGG" id="tsr:106548442"/>
    <dbReference type="CTD" id="23178"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000059">
        <property type="entry name" value="PAS domain containing serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000351">
        <property type="entry name" value="PAS domain-containing serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000346">
        <property type="entry name" value="PAS domain-containing serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="23">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="837"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="866"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="25">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_013921289.1"/>
        </source>
    </evidence>
    <sequence length="1172" mass="130292" checksum="5E1269723BB2CF55" modified="2020-10-07" version="1">ILVANDKACKILGYSTQEMIGRKLSEMISPSNWDIVEALKEGYADLEEHGPVIPGTVVDAISQNNEKIPVTIWTRKIKGHCSQYCVVVLEPVERLSASVSFKSNGEITSCDPLFAHLHGYSSSEDVVGHYIQDLIPSIQLPAPGKKIPKNIKIQRSVGRAREGTTFPLSLKLKVNPLVDEVASVSDDPPPAMDVETLENSTPLPIDCCFSATIWVFTTISGLVTIQADGTIYGINNTFSLMLFGYEKKELLGKNITFLIPGFYKNMDKNDSSLQLPQSRESYVNAENTNSSEGMQKDGCAIGKVTSSFFVDDIDSPNLKEHQELGRNGAKLTPKKEIQLKSLGNVSCALRSPAVVSSSLICGNHIPSHNVLATNEAISSENLYYEDGSEKETGKSAIHQMNELHSLKQNHSESNVFDDRSKLNNCLSRSTYDLIQEENDNLNIHNSTSPQDMKYKPSTTRRGRKHSLLGSEAFDKNLLKAELQLEANVPYEALHGSFGTPTLDEPNDDIASNPRQNHEVPPRTLMNRANSLTFENNSIESSTIDSSVFLPAKKSNSLPIHGGSSQVYDASMVMGSEDVGNNHSVCCGLMNLGINEEVKSDSCAAFGPIKLSFLDAKDLFQSLDWTNNITSDQLELELSDISPSASQKNLFANKPSEYFDSDFSILCTSELESAVPTIRDDLEINSLKSNVESQEDFFTLKQQHMAQVASTPVKLEKVLSPTTALNTEILEGSYAGNCYHRDGSRLSILFEVKRVELHDPAALFCIWVMRDLFQSQKQAAAKTQLLLSSLASSCQTLDEISAVNLAELIKTTPLFENSRKVEELERLKACEGSYGEKYDTLTFIGKGAFGFVWTGKCKMDEKEVVVKFIWKGRVLDYCWVEDPELGTITQEISILKKLQHPNIIKVIDIFENQQFFQLVMEKHGTGLDLFTFIDNQPDLDEPLASYIFRQLVSAVNYLRSRNILHRDIKDENIIIAEDFTIKLIDFGSAAYLEPGKMFYTFCGTIEYCSPEVLSGNPYSGPELEMWSLGITLYTIVFGENPFCELEETIDAVLRPPYNVSVGLMSLLSGLLKPFPQDRITLEMVIRDPWVFQPINVINYAWEKVFTCSKTENNSFKIQPAGCNHGGIWAIPTLDSEQSFNDEVNNHEPANSQLLGIISSAEREPSTSSQCQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC28</accession>
    <name>A0A6I9YC28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras association domain-containing protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">PAM COOH-terminal interactor protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Peptidylglycine alpha-amidating monooxygenase COOH-terminal interactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RASSF9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a role in regulating vesicuar trafficking in cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with PAM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921614.1">
        <property type="nucleotide sequence ID" value="XM_014066139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC28"/>
    <dbReference type="GeneID" id="106548708"/>
    <dbReference type="KEGG" id="tsr:106548708"/>
    <dbReference type="CTD" id="9182"/>
    <dbReference type="OrthoDB" id="10034447at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012510">
        <property type="term" value="C:trans-Golgi network transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000113">
        <property type="entry name" value="ras association domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033593">
        <property type="entry name" value="N-RASSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286:SF10">
        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="11">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="284"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="154"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="413"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00078989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013921614.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="50457" checksum="3432BBAD17509FB3" modified="2020-10-07" version="1">MSPFGRNLLKTRHKNRSSVKEMESSEQEIIVWVCQEEKIVSGLTKHTTCMEVIEALLEEHQTTLAEKRFLFGECKDYCIIEKWRGSERVLPPLTKMLRLWKAWGEELPNLHFVLVKADAFLPLPLWRTSEAKIVPNLEMHLDLSPANYMKMLSVDKQKKILRKTFKKLAKLKQEGVLQERDNIETLIHLILSQDHTIHQQINRMKELDMEIEECEAKFQLESDGENYVQESYLMSSCSSSEQLVEMPHHHQPESSNKYDGILQVEEGLRHHKLMIEKLSAEIEQEVKESEKNGDIRTEEAPKGEGENTDLESVKNELERSMKDALRIHSQLNCIEQELKYRDLMLQRKEKEYELLVEELSGLHAKDKNVFRYPTSEEEMKDNETGTCTKNFPGTDFLQKMTNLDLNDTDSDTGISSTHSQDSETAVGDTLLCPHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCF3</accession>
    <name>A0A6I9YCF3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 106-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921836.1">
        <property type="nucleotide sequence ID" value="XM_014066361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCF3"/>
    <dbReference type="GeneID" id="106548871"/>
    <dbReference type="KEGG" id="tsr:106548871"/>
    <dbReference type="OrthoDB" id="8721206at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031591">
        <property type="entry name" value="CCDC106"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16477">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16477:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15794">
        <property type="entry name" value="CCDC106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921836.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17880" checksum="95C378F503360A56" modified="2020-10-07" version="1">MSPSSQSNNVKERPAPSLTLVTNMKTQLHMALERNSWLQKRIEDLEEERDFLRCQLDKFISSARLDADDHCCNKQLPRRSEILESRNGNMTDDDSMSSWMATSPEEGGSVEHKKLKAGFNHRRFAKPKIRERQRVRSKWSKGTQRCLYKASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE77</accession>
    <name>A0A6I9YE77_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NACHT, LRR and PYD domains-containing protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922344.1">
        <property type="nucleotide sequence ID" value="XM_014066869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE77"/>
    <dbReference type="GeneID" id="106549270"/>
    <dbReference type="KEGG" id="tsr:106549270"/>
    <dbReference type="OrthoDB" id="10058437at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrin" evidence="1">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922344.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="17272" checksum="4EF1B98C261F8C6C" modified="2020-10-07" version="1">MVVQFQCSETTANTFATGGLNGPKPFRKDIDYLEELNEEEFEKFKMHLEDYPVEKGYKPICRSKTEKASHIEIARYMIEAFDDTKAVKMTVSIVDRINKKDLAARIRKEMPEYFLPKPSTMTKKEKKEREKTILSNLHDKFFGIPKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF44</accession>
    <name>A0A6I9YF44_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein C9orf69 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922818.1">
        <property type="nucleotide sequence ID" value="XM_014067343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF44"/>
    <dbReference type="GeneID" id="106549639"/>
    <dbReference type="KEGG" id="tsr:106549639"/>
    <dbReference type="CTD" id="90120"/>
    <dbReference type="OrthoDB" id="10013139at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041156">
        <property type="entry name" value="TM250"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48431">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48431:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17685">
        <property type="entry name" value="TM250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922818.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="16250" checksum="05F4FA333EC25389" modified="2020-10-07" version="1">MPVIPIPRRVRSFHGPHTTCLHSACGPVRTAPLVRTKYNNFDIYLKSRWMYGVIRFLLYFSCSLFTSVLWVALAVLFCIQYLGIRFFLRFQYKLSITLLLMGRRRVDFSLLNELLVYAIHVTMLLVGGLGWCFMVFVDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF59</accession>
    <name>A0A6I9YF59_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Testis-expressed sequence 2 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106549478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013922620.1">
        <property type="nucleotide sequence ID" value="XM_014067145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF59"/>
    <dbReference type="GeneID" id="106549478"/>
    <dbReference type="KEGG" id="tsr:106549478"/>
    <dbReference type="OrthoDB" id="26740at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013922620.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="29682" checksum="20B83FAF0279F4E3" modified="2020-10-07" version="1">MNEIYNYDPETYHATLTHSIFVRLEGSILRLSKPNKNISRKALYNETKPEVTYVSQKIYDLTDSKIYLVPKSLARKRVWNKKYPICIELGRQDNFMAKAQIDKETSEEKISLEKENSEASLDGAKSGTQKNQVLYLFARTGRDKEEWFRRFLIAHKLKFEEKKPIVVWGSKSGLLPGHSRSNSQSGILTHSRSSSKGSVEEVLPQPKLKDLGLNIRQKILLDYNVYMARCIPPEHPSPTVSPIPSADSSPTAVKKVKYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFB5</accession>
    <name>A0A6I9YFB5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha/beta hydrolase domain-containing protein 17C</fullName>
            <ecNumber evidence="3">3.1.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ABHD17C</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Postsynaptic density membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the AB hydrolase superfamily. ABHD17 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013922871.1">
        <property type="nucleotide sequence ID" value="XM_014067396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFB5"/>
    <dbReference type="GeneID" id="106549690"/>
    <dbReference type="KEGG" id="tsr:106549690"/>
    <dbReference type="CTD" id="58489"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099175">
        <property type="term" value="P:regulation of postsynapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000008">
        <property type="entry name" value="Alpha/beta hydrolase domain-containing protein 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF55">
        <property type="entry name" value="ALPHA_BETA HYDROLASE DOMAIN-CONTAINING PROTEIN 17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="17">
        <location>
            <begin position="148"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013922871.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="38409" checksum="37DF40063D32C2FF" modified="2020-10-07" version="1">MKRVASDLRRLKGNNVSSGIVSRVLFLSLSRCGFFHPASLMPEQGPRMNGFSLGELCWLFCCPPCPSRIAAKLAFLPPEPTYTVMQLEQQESAATVGTPTSSCSLHLTERADWQYSQRELDAVEVFFSRTARDNRLGCMFVRCAPSSRYTLLFSHGNAVDLGQMCSFYIGLGSRINCNVFSYDYSGYGVSTGKPSEKNLYADIDAAWQALRTRYGVSPENIILYGQSIGTVPTVDLASRYECAAVILHSPLMSGLRVAFPDTRKTYCFDAFPSIDKISKVTSPVLVIHGTEDEVIDFSHGLAMYERCPRAVEPLWVEGAGHNDIELYAQYLERLKQFISHELPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFX4</accession>
    <name>A0A6I9YFX4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane emp24 domain-containing protein 9 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TMED9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922815.1">
        <property type="nucleotide sequence ID" value="XM_014067340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFX4"/>
    <dbReference type="GeneID" id="106549636"/>
    <dbReference type="CTD" id="54732"/>
    <dbReference type="OrthoDB" id="3427at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027012896" evidence="11">
        <location>
            <begin position="28"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="37"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013922815.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="26360" checksum="2177EDA241A5AACD" modified="2020-10-07" version="1">MAAPGGRSRVLLLLLVVAAGLAPRAWGLYFHLGETERKCFIEEIPDETMVIGNYRTQLYDKQREEYLPATPGLGMFVEVKDPDEKVVLSRQYGSEGRFIFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLLEQIEQIQKEQNYQRWREEQERFRQTSESTNQRVLWWSIVQTLILVIIGVWQMRHLKSFFEAKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG24</accession>
    <name>A0A6I9YG24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">OXR1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in protection from oxidative damage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923146.1">
        <property type="nucleotide sequence ID" value="XM_014067671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG24"/>
    <dbReference type="GeneID" id="106549912"/>
    <dbReference type="KEGG" id="tsr:106549912"/>
    <dbReference type="CTD" id="55074"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.350.10:FF:000002">
        <property type="entry name" value="Oxidation resistance protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF69">
        <property type="entry name" value="OXIDATION RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="7">
        <location>
            <begin position="74"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="8">
        <location>
            <begin position="672"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="333"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="412"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="427"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013923146.1"/>
        </source>
    </evidence>
    <sequence length="753" mass="84250" checksum="04AE5EBD35E5C791" modified="2020-10-07" version="1">MEYLTTFTGKSGRLLRGTANRLWGAVPGAGSLRQVHFQDSQHDGTPSPPDTGQKKTIDKKDGRRMSFQKPKGTIEYIVESRDSLNTVALKFDTTPNELVQLNKLFSRAVVPGQVLYVPDPEYLSSVESSPSLSPESPLSPTSSEAEAEKTADSDGAQKKETAAIPAYPCVRSTRVVSSTSEEEEAFTEKFLKINCKYITSNKGTVTGVLLVTPNNIMFDPHKTDPLVQQNGCEEYGIMCPMEEVMSAAMYKDADVKIQDSLPIDVGQLSVKDLCHFKKITRSNTDEMELRMRDTGNDSASTAPRSTEESLSEDVFTESELSPIREELLDDLRQDKSSGASSESVQTVNHTGGESVKDISDSVNTIGDVNSSGEQPLDDGSTSVNDTVDSKAVEEAIKIQNIVEGSISDAEAQHGTCQEPVEGNVPEDEQHLDSEKTDSQKEVTNEGETVKLPTQGSETDTETLRTLWKTHTMQQTKQQRDTIHQEVHKEMKHKMATVDGTVEGPLIKEKRRHRLYKFLCLKVGKPMRKTFVSQASALMQQYAQRDKKHEYWFAVPSERTEHLYAFFVQWSPELYTEDTGESNREPGFIVVKKIETQAGEDSVDDETAIKDWEVVSVAEYLRRIDALNNEELRTLCQRLKITTREDTCSKQETTIKTELEPEAFRPNLSDPSELLQSDEIEKLTKQLPPRTIGYPWTLVFSTAKHGMSLKTLYRTMAGLDTPVLLVIKDSDGQVWKLQWPLFLSFAPHFPHNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGF5</accession>
    <name>A0A6I9YGF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">LOW QUALITY PROTEIN: olfactory receptor 51H1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106550090</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923358.1">
        <property type="nucleotide sequence ID" value="XM_014067883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGF5"/>
    <dbReference type="GeneID" id="106550090"/>
    <dbReference type="KEGG" id="tsr:106550090"/>
    <dbReference type="OrthoDB" id="6144443at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF53">
        <property type="entry name" value="OLFACTORY RECEPTOR 51H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013923358.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="35878" checksum="E98170DCBDD78A15" modified="2020-10-07" version="1">MFDNSSLNLRTFVFSGIPGMEEKNALMAFLLFVLCVFILLGNSLILFVIKTDQSLHEPMYIFLSILACSDLGLSMSTLPTMLKVYWCNWKEITFNACLTQMFFIHAFQWLESGVLVAMAFDRVVAIREPLRYRTLFPHTVVAKIGITAAVRCICVCFPAPFLIKRLHFCRSNILSYPYCFHPDVMKLSCVDIKVNILYGLIVMIFTLGFDVAFILVSYIMILKTVLGIASHQERLKALNTCISHICAILIFYAPIISICLLQQYGKHLSPLVHVVMANAYIVVPPLLNPIVYSIKTQQIRQRIYKIFPRKQTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGJ6</accession>
    <name>A0A6I9YGJ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uroplakin-3b-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923302.1">
        <property type="nucleotide sequence ID" value="XM_014067827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGJ6"/>
    <dbReference type="GeneID" id="106550047"/>
    <dbReference type="KEGG" id="tsr:106550047"/>
    <dbReference type="OrthoDB" id="9939598at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024831">
        <property type="entry name" value="Uroplakin-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15446">
        <property type="entry name" value="UROPLAKIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15446:SF15">
        <property type="entry name" value="UROPLAKIN-3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923302.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="14830" checksum="0D242F2071CF6896" modified="2020-10-07" version="1">LDVFSEVVPYTPRVSDMNMAGKVTVTTFALESPKCVFDSFINASDTIWLVITFANAEIWKTIDTWPGRRSGDMIVITVILSALCGVVTVGFLSTVCYEWHMARTAPARCSVEQLKHRGVSQRRLNACTRCVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHM8</accession>
    <name>A0A6I9YHM8_9SAUR</name>
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        <submittedName>
            <fullName evidence="7">Claudin domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CLDND1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923365.1">
        <property type="nucleotide sequence ID" value="XM_014067890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHM8"/>
    <dbReference type="GeneID" id="106550096"/>
    <dbReference type="KEGG" id="tsr:106550096"/>
    <dbReference type="CTD" id="56650"/>
    <dbReference type="OrthoDB" id="9885915at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042356">
        <property type="entry name" value="CLDN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14347">
        <property type="entry name" value="CLAUDIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14347:SF3">
        <property type="entry name" value="CLAUDIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013923365.1"/>
        </source>
    </evidence>
    <sequence length="264" mass="29426" checksum="30A45303C76A6B67" modified="2020-10-07" version="1">MMDNRFATALVIACVLSLISTIYLAASIGTDFWFEYRNPSPPENNSESKATWDELFGENADENSYTEALFRWNGTIGLWRRCITMSENSHWYNSPGFNQFLPNLNTTSNKTGEMVTKCISFSLSDQFMEKYNVPGNHNSGMDPNRTYLWRLQFLLPFVSLGLMCFGALIGVCACACHSLYPAIATGVLHFLAGLCTLGSVGCYVAGIELLHKKLPPPPVQGQFGWSFCLACVSAPLQFMAAALFIWAARTNRKEFTLLKAYRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJ27</accession>
    <name>A0A6I9YJ27_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Muscleblind-like protein 1 isoform X5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MBNL1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
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            <taxon>Natricinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the muscleblind family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924247.1">
        <property type="nucleotide sequence ID" value="XM_014068772.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="106550776"/>
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    <dbReference type="OrthoDB" id="6285980at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000004">
        <property type="entry name" value="Muscleblind like splicing regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000002">
        <property type="entry name" value="Muscleblind-like 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054429">
        <property type="entry name" value="Znf-CCCH_Muscleblind-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF7">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22628">
        <property type="entry name" value="zf-CCCH_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013924247.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="34121" checksum="B0B4CD2746BA5BE7" modified="2020-10-07" version="1">MAVSVTPIRDTKWLTLEVCREFQRGTCSRPDAECKFAHPSKSCQVENGRVIACFDSLKGRCSRENCKYLHPPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLANAMMPGAPLQPVVCREYQRGNCNRGENDCRFAHPADSAMIDTNDNTVTVCMDYIKGRCSREKCKYFHPPAHLQAKIKAAQYQVNQAAAAQAAATAAAMGIPQAVLPPLPKRPALEKTNGATAVFNAGIFQYQQALANMQLQQHTAFLPPGSILCMTPATSVVPMVHGATPATVSAATTSATSVPFAATATANQIPIISAEHLTSHKYVTQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJE8</accession>
    <name>A0A6I9YJE8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dynein heavy chain domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106550859</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924316.1">
        <property type="nucleotide sequence ID" value="XM_014068841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJE8"/>
    <dbReference type="GeneID" id="106550859"/>
    <dbReference type="KEGG" id="tsr:106550859"/>
    <dbReference type="OrthoDB" id="5986589at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026935364" evidence="2">
        <location>
            <begin position="18"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013924316.1"/>
        </source>
    </evidence>
    <sequence length="646" mass="72960" checksum="BD2D6A56B49EDBD1" modified="2020-10-07" version="1">MLPLALAAALPVDFACAARPSLESAEVASPAPAPGLPVAAPPADRQAPMSGFQVAEVLSGRYHAGEIRFFYLNTAPNRHFRPYDLVTVPGYLIKPQHYVFSPFGVLCVHPEEGSVALSLGDWHREARLWQLMQHIPFFRLFLVRKAFTRWCVNVKYLQYLKLREKLNFQLLQAVPHFGAALLHISRLLQELQSVHWLPTGASRCYNFIELKRSLAQMNSNADGFLHRFLALCTSILELVRDDTYKMVHGLQTEVQGYRLYITKESPYQQRLEFERLQCRLREAESWLQMLGTLAMLVNFLICQTLISVMHQEASSFVNFTMQADGSTQKAVLRVQLVFSADNQLVVFPSSGELEDCLLGALQTMVETVLETTRVKSEKAEAQAGPRTPETERPPSQAAGDVAPAAPGDDQISRLREQLQQMSLTVLCSDKAQLVHRLDLKACSGLQVVGHRLRAEYPLMSREQLETDLHTDSIIQAAMAKLQDLFMAGLAETQLLCREYSWLGHIYQYVHSWSDGQLESMKGLPAEEYVNQILKLRTWAVQVQKVPLVVITFNRFFLVDCSGILQDILPLLASIDEDILALLLSEITERSAQFITELASVLQLYMNVGTDIFTIAKCSQKTSKWRFLSVDDYWMYLAGFIFLQTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJG4</accession>
    <name>A0A6I9YJG4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RNA polymerase II elongation factor ELL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ELL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924387.1">
        <property type="nucleotide sequence ID" value="XM_014068912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJG4"/>
    <dbReference type="GeneID" id="106550890"/>
    <dbReference type="KEGG" id="tsr:106550890"/>
    <dbReference type="CTD" id="8178"/>
    <dbReference type="OrthoDB" id="6284217at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF9">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="8">
        <location>
            <begin position="456"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="255"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="340"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="430"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924387.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="63683" checksum="BB572D54A8A5D436" modified="2020-10-07" version="1">MAALTERGYVLSCGRLGRGTRISVFHVKLTDSALRAFEAFQGRKDSVGLKPSIQFQGSQGHISIPRPDCPTGVRTFSFYLSNIGKDSPQGSFDCIQQYVSSNGNIQLDCLGSVQDKITVCATDESYQKAKQSMALVEEETRSRGAIVIKPGGRYVGKTVKMRKPAPGVSDTVPSRIRPTPVNLASAIKKGNSAVSQRPFKDRVMHLLALKPYKKPELLLRLQKDGLSLQDKDSLDSLLQQATLVCSFPRKLFQPQNPSCIPENSAPSPPRDTAHVSSSPPQKRSQPLDFIDPLANKKPRISHCAQRTQPTFNGKLNSANGKETALPTMVAANSVSSSSLLPTLELPRPHDPLADVSNDLNHNGKDFEMSEPAERRTASRSAADPAQMSKHNCAPHSKSRKKKHKERERKEEQQQVHEKPNCELKKGGSKMSPSQDSAGLNRTCNSSSVPSSTSGRPDYLLKYGAISSSEQRQSYKNDFNAEYGEYRDLHARIERITRRFMELDSELKQLSQGSEEYKTIHDQILQEYRKIKKTNPNYSQEKNRCEYLHNKLAHIKKLIAEYDQQQIQSWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJG6</accession>
    <name>A0A6I9YJG6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106550515</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923920.1">
        <property type="nucleotide sequence ID" value="XM_014068445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJG6"/>
    <dbReference type="GeneID" id="106550515"/>
    <dbReference type="KEGG" id="tsr:106550515"/>
    <dbReference type="OrthoDB" id="6151005at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000037">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013923920.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="41313" checksum="7F90A287FB12C211" modified="2020-10-07" version="1">MENQTTVLYFLLMEFSNNRYLQLLHIFLFFILYLATAMTNLFIITVIAFDHQLHNPMYFFLMNLALQDLGIVSVIVPKSMINSLIGTRQISYFGCVAQIFLFISFEAFELSLLTVMAYDRYVAICRPLYYELLMNWKVCIKIVILVWVTGILYGMLHTTGTFYIPLCSNVVNQFFCEIPHLLNLACFDFNLLEIGLLGISITTGLGCSIFIIISYVMIFKVVLRIPSVKGRQKTFSTCLPHLIIFCILLNIGFISENMRAYSSLDEELRVPNYSTTMFSGCYSKLSPIKTIYLPHFKISTELVMIIGFISEGTTVYSAVNTWFISDEELVGNIREVLGKYALEYSPYECGNATMVVNVRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJP4</accession>
    <name>A0A6I9YJP4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">USP30</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013924593.1">
        <property type="nucleotide sequence ID" value="XM_014069118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJP4"/>
    <dbReference type="GeneID" id="106551102"/>
    <dbReference type="KEGG" id="tsr:106551102"/>
    <dbReference type="CTD" id="84749"/>
    <dbReference type="OrthoDB" id="2248014at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF888">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="69"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013924593.1"/>
        </source>
    </evidence>
    <sequence length="502" mass="56384" checksum="CFD59C44D7395C9B" modified="2020-10-07" version="1">MVLSRFGRLETERATAVLRRLLRAGGWARSRAMRNWGVIGGVAAVLAAGLYVLWGPMAERRRRRKGLVPGLLNLGNTCFMNSLLQGLSACPSLIQWLEGFAGQDSQAGQSKKAGQRSLSLTLLRLLKALSCQVATEDDLLDASCLLEVLRLYRWQISSFEEQDAHELFHVLTSSLEEERDGQSQIPHLFDVHSLEEPEIPQEQICCRTKGLLYPVPNRWKRQHPFHGRLTSHMVCKRCKQQSPLRYDTFDSLSLSIPATVWGHPLTLDHCLRHFISSESVKGVVCDNCTKIQAEGTKGLIITEHHRTTFVKQLKLGKLPQCLCIHLQRLSWSSQGLPLKRNEHVQFSEFLIMDLYKQHFPTHKPSPREGLRAEPKPGQGGEQAGNSKAVLSNGTCSTPWLPPSIGFPYMAVPGCGSPIYVYRLMAVVVHHGDMHSGHFVTFRRSPSSSRTPPSAGSQWLWVSDDVVRKSSLQEVLASSAYLLFYERICSRLQPPTRELQAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLK3</accession>
    <name>A0A6I9YLK3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">E3 ubiquitin-protein ligase MARCHF6</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Membrane-associated RING finger protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Membrane-associated RING-CH protein VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">MARCH6</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 25">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with DIO2. Interacts with SQLE.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013924655.1">
        <property type="nucleotide sequence ID" value="XM_014069180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLK3"/>
    <dbReference type="GeneID" id="106551153"/>
    <dbReference type="KEGG" id="tsr:106551153"/>
    <dbReference type="CTD" id="10299"/>
    <dbReference type="OrthoDB" id="1108038at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16702">
        <property type="entry name" value="RING_CH-C4HC3_MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000096">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="370"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="22">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="210"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013924655.1"/>
        </source>
    </evidence>
    <sequence length="565" mass="63975" checksum="E7B4059A667D9630" modified="2020-10-07" version="1">METAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPQWLEQNQQQAINGVGQQNEAQGVGNAGAENAVLDQPADQPAENVVVGENPEILEEQPDEEEEDNEDEEDVVVEDAADANNGGQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSIVGLGFEEYVQASHFEGLITTIVGYVLLAVSLIVSHGVAALVKFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKYLLPHFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRGWTVTAGYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLL7</accession>
    <name>A0A6I9YLL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NUP85</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
            <location evidence="1 10">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925263.1">
        <property type="nucleotide sequence ID" value="XM_014069788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLL7"/>
    <dbReference type="GeneID" id="106551645"/>
    <dbReference type="KEGG" id="tsr:106551645"/>
    <dbReference type="CTD" id="79902"/>
    <dbReference type="OrthoDB" id="17644at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8 10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013925263.1"/>
        </source>
    </evidence>
    <sequence length="648" mass="74671" checksum="E76A879420C8413F" modified="2020-10-07" version="1">MEELDVVTLIPGVNSDQKQLCFTWGPTEMLVCETLFRPTETKEKTPGCPFVYVVRKDQDVYSQTLRKLFNESHGIFVGLQKNEKEKAGKSRTAQLVGVSKSYRSVIRACMEDSHQMAISARDPAMHMHYSTQVSVLSAMELIWNLCEIMFIEAAPAGLVLCHLLDWVRLHVCEIDNMVCEVLRSENPAKHEHFWNVVTGYVLQGRMDEARQLLSKEASTNPTSVHMCKVLDELMKKMPVLSPSSNQTLTEMELKWQHWHEECERFLKDGTFASNSHMEVLCKILLGDENAILEKKELMTTWYHFLVTRLLYCHPTVKHVELHLYAQSSLDMFLGGESSPEPLDSILLAAFEFDIHQVIKECSIALSNWWFVAHLTDLLNHCNLLQSHNLYFGSNMREYLLLEYASGLFAHHSLWQLAVDYFDYCPEYGKAYLEHHIERISLDTEHKALKVLRICEQRTMTEQVRSICKIMSMKAVRNNRLGSALSWSIRAKDAAFATLISDRFLREYCERGTFSDLDLIDNLGPSMLLSDRLTFLGKYREFHRMYGEKKFFAAAKLLLMLMTARIAPCSFWMTLLTDALPLLEHKEVIFSADQTYELMKCLEDVMAAEPKKEKLQDDDAEIMKVEMLRLALARNLARAIIKEGTLEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN07</accession>
    <name>A0A6I9YN07_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lipoxygenase homology domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106551551</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925145.1">
        <property type="nucleotide sequence ID" value="XM_014069670.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106551551"/>
    <dbReference type="KEGG" id="tsr:106551551"/>
    <dbReference type="OrthoDB" id="5322100at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23312">
        <property type="entry name" value="beta-trefoil_FGF_RP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01756">
        <property type="entry name" value="PLAT_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052970">
        <property type="entry name" value="Inner_ear_hair_cell_LOXHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901:SF7">
        <property type="entry name" value="OXYGEN-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901">
        <property type="entry name" value="PROTEIN CBG12474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="129"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="561"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="687"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="822"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="952"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="1088"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="1218"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="1475"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="1731"/>
            <end position="1849"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="2024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925145.1"/>
        </source>
    </evidence>
    <sequence length="2024" mass="229040" checksum="55B797FF8B7F40BD" modified="2020-10-07" version="1">MKWKVVIFTSDQPSSGTSSQVYITLYGEQGNSGAVFLYGEEKKVFERGSKDTFMIHTEDLGELYKIRIGHNNSGETPAWHCKELQLQNIVTGEKYDFSVEKWLTQAQDEGKISQELPVLHQGYPVLPVAMYKVHVVTGDLWNAGTEADVYIAINGEKGDTGCRQLHRSMKSQQHMKGQTDTFSLEAVHLGHLYKIVIGHNGLGSGNGWFLEKIVVQDPITNSDYIFLCYRWLDQGEDDGKIVRELYVIDNYTIAMKQELEIKRKNMWAAEKWKFQKGNILQLYNKMTHGFIRLRRDGVVDAVGDKKDKYGLFEVAAKKGSLCVLRSHPNPQLALALAHGSVTGMNYGDNHCELQVHVQPNHCTILESSQKPGQMISFNFQGTVADDNTGYAGLTKEFVVHVKGIFHNSAIILLATSYCQFLCLRSDGSCSGAGNQADDSSWKVHKISSGICMFENVKHPRMYLRIKDGQCNGTGIGDVDCHFKVQKNLETVSVSLESAQNRGIYVGLLPNGETKPLVDTGESNVLFYPQVVKCSTCICVFGGKQKEMMTIQNEKSPFPSEDKWKVFILTGNSGTQANVTLWIYGDKGSVGPIILGNDNREQLFLPRTEDAFQVEMKDIGNIYKIRIGHDGSSQYADWMLEKVTLQHMKTGRILKFPTNKWLSWNEGNGNSVCELSAAENGRSLYPIVSYQVYVSTGHLEHAETTSSIYLCVYGERGDSGLRLLAKSDQPIKFQRGMIDMFEIQAVSLGHLQKVLLCCKSDNQLQDWYCEKIVIRESGKENIFICQRWLPYMSQGIIHSEIELFVQEMQINHQLKMQEEENGEDWKITIVTGGFETAGTTATVSLYVYGDNKASGPLILGSGKHQLFNPNSTDTFQINLKNIGEPYKIRIGHDNSGTNPSWYLNEIRFQKMNLPSEQEICFSVNCWLSEDQGDGDTWREIPIRRPKKELLPLVVYEINVYTGTQPGAETNSNVYITIYGNQGDSGKRKLQKSSTNKVTFQCGQTDVFSVTAVSLGVLHKIVIGHDGIGPGSGWFLQKIVIRFQEKAVSKEEVIEFLCNRWLDEYQEDGKTERELIARKNSNASMAFLRGQWRVMVKTDHDSPEPRTSKRTLVIYGSKGKSDDLLLSSQSLGYVCFQPGATDEFIIESKDVGDVYKIRLNCDDLVDFESWHLKSLQMKELSGRQEVNFDCNCWFSHSQECKDMVKEFPALNEIQNTLSVHKYIVSVHTGDLWGSETFANIYITVYGERGDTGVRKLQDSFIEGDKFQRNKVDSFLIEAVSLSHLKKIVVGHDGEGYGAGIYLKMVTIKKSETSDKEWVFPCWNWLDTHMGICDTVCEFKTIGKRRISSSRYSPINQQISGLWIMDITGSDVNAETNPMHFTFNFYGDKGHKKLALQLLGKTTQIKDELTNVGSLFKIQISSDQAQLKESWHMNMLHMKHTDTKQEMWLYFDYWFKPNEDTCVELPVFFAGQDPLLVVEYSIQVHTGDRKKAGASGNVYFCICGDRGDSGKQWLNNSKTGPITFDRGQMDVYQIKAVHLGKLNQVIVGFKGLNKDDWFLEKIVIEERNYPFTTYTFVHNNWLHQQSKKDFTEASIPLQEMTEKSGPMKGFEAKSQGQWRLWIDFVHVPEKMPEIKILAYGTNGVSFAQRVQHFKNEPFLLSIGDIGQITKLSFLSSCSNLNKGIKLHKVRMKDLDTKEELGFHPLNRWLFEEDGSETVTELAVVRPNEAPLKEITYSINVYTGTLPASETDANIFITLLGKNGDSYKRQLKHPKSCPQFEKGQISTFAIQAVDLGMLSSVLVEHNNSGYGAGWYLDQILIQELGKSNSQYVFSCQRWLDSGVGDGKMKQELWLLGKTNKESLTKDVHGIWDVTVIAGEMSDNYGNSKLILTVCDDKGTSAAVFIPKGSFKRAKIYQTSLELDKKFNKICKIRLEVEDSDRETWHCHEVKLQNKKNKETLEIPCYRDFPDNKGCTVVEFPILITGTRFLTGLLFTKQLKQLPQKLIKDICLLCGVEKSHKKETGMKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YN51</accession>
    <name>A0A6I9YN51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein phosphatase inhibitor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PPP1R2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein phosphatase inhibitor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925190.1">
        <property type="nucleotide sequence ID" value="XM_014069715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YN51"/>
    <dbReference type="GeneID" id="106551586"/>
    <dbReference type="KEGG" id="tsr:106551586"/>
    <dbReference type="CTD" id="5504"/>
    <dbReference type="OrthoDB" id="551302at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="169"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013925190.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="25373" checksum="777D64EB7BBF9E70" modified="2020-10-07" version="1">NRSDSGSGSAGRGLADTEALSQASPLPPAAPLLQAQPSTESPGEEQGKKTQKWEEMNILATYHPPGKDYGLMKIDEPSTPYHSLKEEDAGSDAEDSSGPTPDVLAEKLAAAAQGQGPKILARQDYSSDEEEEDDEVDLTPEELEKKRQFEMKRKMHYNEAQNIKLARQLIAKEMHGEAANEEEDCEEDEEEDEDNEDDMQDECKTDTESVPAPGDPLENIEHSLEEVCSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YP34</accession>
    <name>A0A6I9YP34_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Junctophilin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106552225</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the junctophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925921.1">
        <property type="nucleotide sequence ID" value="XM_014070446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YP34"/>
    <dbReference type="GeneID" id="106552225"/>
    <dbReference type="KEGG" id="tsr:106552225"/>
    <dbReference type="OrthoDB" id="284854at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030314">
        <property type="term" value="C:junctional membrane complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000001">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000003">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000012">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017191">
        <property type="entry name" value="Junctophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085:SF7">
        <property type="entry name" value="JUNCTOPHILIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013925921.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="41822" checksum="737F59A6CE0DFDF3" modified="2020-10-07" version="1">MSSGGRFNFDDGGSYCGGWEDGKAHGHGICTGPKGQGEYAGSWSHGFEVLGVYTWPSGNTYQGTWAQGKRHGIGVESKGKWVYRGEWTHGFKGRYGVRECTGNGAKYEGTWSNGLQDGYGTETYSDGGTYQGQWAGGMRQGYGVRQSVPYGMAAVIRSPLRTSINSLRSEHTNGTALHPDASPAVAGSPAVSRGGFVLMAHSDAEILKSKKKGIFRRSLLSGLKLRKSESKSSLASQRSKQSSFRSEAGMSTVSSTASDINSTISLGEAETEISVIEDDIDATTTEIYVGEWKNDKRSGFGISQRSDGLKYEGEWATNKRHGYGCMTFPDGTKEEGKYKQNILVSGKRKNLIPLRASKIREKVDRAVEAAERAATIAKQKAEIAASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ96</accession>
    <name>A0A6I9YQ96_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Hemojuvelin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">LOC106552165</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013925850.1">
        <property type="nucleotide sequence ID" value="XM_014070375.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013925851.1">
        <property type="nucleotide sequence ID" value="XM_014070376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ96"/>
    <dbReference type="GeneID" id="106552165"/>
    <dbReference type="KEGG" id="tsr:106552165"/>
    <dbReference type="OrthoDB" id="10013795at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010536">
        <property type="entry name" value="RGM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF3">
        <property type="entry name" value="HEMOJUVELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06535">
        <property type="entry name" value="RGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044636652" evidence="10">
        <location>
            <begin position="41"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule N-terminal" evidence="12">
        <location>
            <begin position="42"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="11">
        <location>
            <begin position="151"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013925851.1"/>
        </source>
    </evidence>
    <sequence length="410" mass="46316" checksum="8BA8F5D7EE3DF0E5" modified="2020-10-07" version="1">MTEWFKRRLSACCMNPLYLMNPSFFIKLLVLLLLYKHVDSQCKISRCNSEFIEATADMHDSERSTAYCNALRDYSSCTRRTSRTCRGDLAYHTAVYVIEDLIIQNNCSRDSPTTVPKPPALSPNKHDYFPSTDTCDYEKSFIHKHGQSPTYQYCAVFGDPHVRTVNDEFHTCRVEGSWPLLDNNNLFVQATSYPIIKGSNVTGIGKLTIIFKNMKECIEEKVYQAEMDNLPVAFADGSVNGGEKPGGNSLTIHEHIPGQHITIQATYIGTTIAVRQVGKHLSFSIRIAKEIATSFTDEQDLQLCMSGCFLSQRISIHNCCRPKGSLTRKKARLLCKEKLPVEDIYFQSCVFDVMTSGDVNFTQAAQKALEDARALHPDSKKIHIFHSNRTSYPCSSSFLLLILTILQLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRC0</accession>
    <name>A0A6I9YRC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106552672</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOP53 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926479.1">
        <property type="nucleotide sequence ID" value="XM_014071004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRC0"/>
    <dbReference type="GeneID" id="106552672"/>
    <dbReference type="KEGG" id="tsr:106552672"/>
    <dbReference type="OrthoDB" id="5072at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="42"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013926479.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="29827" checksum="D1EDB29A105457D0" modified="2020-10-07" version="1">MEIISPGGSYNPSFQSHQALLLQAFEAEVARQKAEERLQRQLKFPTAAEAPTQESVFQEQCEGLMEEESEGEEGQGSPPGPLEQDPQAVSDEASPLFTATVVREKKTEQQRKKEKAAKRLKARELSEKAARLRKQELFQLKSIRLQLKQREAELQRRKQRREAKRKLEAIKPKRLGRLKYEEPDVDVQLSSELAESLRTLRWCPMMRPILALGEFAAAVAGNVKDPRGAPGPTEWVRIRELRHPPAPPPPYPPFSAWRAGLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRI1</accession>
    <name>A0A6I9YRI1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cytochrome c oxidase polypeptide VIa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106553024</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase subunit 6A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926917.1">
        <property type="nucleotide sequence ID" value="XM_014071442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRI1"/>
    <dbReference type="GeneID" id="106553024"/>
    <dbReference type="KEGG" id="tsr:106553024"/>
    <dbReference type="OrthoDB" id="5947505at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00925">
        <property type="entry name" value="Cyt_c_Oxidase_VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.95.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.95.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001349">
        <property type="entry name" value="Cyt_c_oxidase_su6a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018507">
        <property type="entry name" value="Cyt_c_oxidase_su6a_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036418">
        <property type="entry name" value="Cyt_c_oxidase_su6a_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02046">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81411">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01329">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013926917.1"/>
        </source>
    </evidence>
    <sequence length="97" mass="11565" checksum="31366655FAE89387" modified="2020-10-07" version="1">WSYILIYCKEITLLDFLLVFEFNPLARLWKILSFVIALPSVAICQLNCYLKKEEHERPEFIPYKHLYIRTKPFPWGDGNHTLFHNPHVNALPTGYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWN3</accession>
    <name>A0A6I9YWN3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Muscle-restricted coiled-coil protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MURC</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Caveola</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CAVIN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928015.1">
        <property type="nucleotide sequence ID" value="XM_014072540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWN3"/>
    <dbReference type="GeneID" id="106553948"/>
    <dbReference type="KEGG" id="tsr:106553948"/>
    <dbReference type="CTD" id="347273"/>
    <dbReference type="OrthoDB" id="8924144at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026752">
        <property type="entry name" value="Cavin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15240:SF4">
        <property type="entry name" value="CAVEOLAE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15240">
        <property type="entry name" value="CAVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15237">
        <property type="entry name" value="PTRF_SDPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="225"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013928015.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="40196" checksum="35153E44E4F06010" modified="2020-10-07" version="1">MDHRATLEIDKIHPNRQPSIADDEEEQDAAFTIVRVLDKVATIVDSVQASQKRIEQRHRDMEDAIKTIQIDILKLAQAHGNTGYTVNKLLEKTRKVSARVKEVRARVERQNVNVQKVETKQEEMLRKNKFRVVIYQEDIQCPSSLTVVKERTQGESTEDAFCPPDDLSSDEECYIEESRTARFKKSGMRRINVIKKAFSRENLQKTRQNFGNKVDRLRTRIVTPERRERIRQSGERLRQTGIRFKKNIANAAPTKKNLKKNRKTTEQPTDGQQGTQESGTETATESREAEPTTEEISYTEVIMKVKKDKGDGSKHLSQADENVMMPPEKNLIKPEMKEDALLLDLKQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXE5</accession>
    <name>A0A6I9YXE5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WASH complex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">WASH complex subunit strumpellin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106555085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the strumpellin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929328.1">
        <property type="nucleotide sequence ID" value="XM_014073853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXE5"/>
    <dbReference type="GeneID" id="106555085"/>
    <dbReference type="KEGG" id="tsr:106555085"/>
    <dbReference type="OrthoDB" id="565118at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140285">
        <property type="term" value="P:endosome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051125">
        <property type="term" value="P:regulation of actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019393">
        <property type="entry name" value="WASH_strumpellin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15691">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15691:SF6">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10266">
        <property type="entry name" value="Strumpellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013929328.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="38504" checksum="93241616E8F9E5B5" modified="2020-10-07" version="1">MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFVPPVFRLKDKSDQQKYGDIIFDFSYFKGPELCEGKLEAKPELQDLDDEFRENNIEILTRFYLAFQSVHKYIVDLSRYLDDLNEGIYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSADSNMDDICKLLRSTGYSSQPGAKRPANYPESYFSRVPINKIFISMVIGRLRSDDIYNQVSAYPLPEHRSTALATQAAMLYVTLYFDPSILHTQQAKMREIVDKYFPDNWVISIYMGISVNLGEAWEPYKAAKTALNYTLDLSNVKEQASRAAAVTERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY43</accession>
    <name>A0A6I9YY43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="7">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106554756</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013928964.1">
        <property type="nucleotide sequence ID" value="XM_014073489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY43"/>
    <dbReference type="GeneID" id="106554756"/>
    <dbReference type="KEGG" id="tsr:106554756"/>
    <dbReference type="OrthoDB" id="59470at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1960:FF:000001">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000001">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1960">
        <property type="entry name" value="QSOX sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040986">
        <property type="entry name" value="QSOX_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042568">
        <property type="entry name" value="QSOX_FAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039798">
        <property type="entry name" value="Sulfhydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22897">
        <property type="entry name" value="QUIESCIN Q6-RELATED SULFHYDRYL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22897:SF6">
        <property type="entry name" value="SULFHYDRYL OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18371">
        <property type="entry name" value="FAD_SOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="9">
        <location>
            <begin position="96"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="248"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="267"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013928964.1"/>
        </source>
    </evidence>
    <sequence length="454" mass="52392" checksum="B88A77D2CDD66613" modified="2020-10-07" version="1">MADLESAVLYTLRMEAAVFPSLEGERLLALKDFLSVLVQYFAGRLVVKNYLYNLELWLRPKTTVLQSEWEEALKNKKEFPNATLPERPHWVGCQGSEPKFRRFPCGLWTLFHHLTVQAACQKQPAPSGVPEVLPTMRGYIHNFFGCRMCAEHFEAMAAESMHEVKTRDEAVLWLWSRHNRVNARLAGTASDDPKFPKIQWPPQSFCPSCHHTSTDSSLQDGKKSLWNENRVLMFLKWHFSGKNIYVDSPLEPQTRSGRSIAQQNDEAEWKLEGGGRGEEGDQDNQKEAGKVGKKKFDLGKPGSPELHKPSIVKMSTKAKEMVEDIVDLDTFSEQRFKSKALKLARQISIRERRAKRDTRLFLMENEARRSLDYIRESLRHKNLGARQFSGIQVEKENTSKRNQWIYILGMGFSRLDVSLCVLLYLLSSVCLLGMYTFFRMRMNYRKARLGYSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYC2</accession>
    <name>A0A6I9YYC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Voltage-dependent R-type calcium channel subunit alpha-1E-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106554841</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929049.1">
        <property type="nucleotide sequence ID" value="XM_014073574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYC2"/>
    <dbReference type="GeneID" id="106554841"/>
    <dbReference type="KEGG" id="tsr:106554841"/>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF5">
        <property type="entry name" value="VOLTAGE-DEPENDENT R-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="88"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="140"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="382"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013929049.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="51208" checksum="0E941017B06C6A48" modified="2020-10-07" version="1">MYNGIRSQGGVSSGMWSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPAIERERRRRHHMSVWEQRTSRLRRHRQMSSQEGINKDEPPLLNPNASIFRKRKLLENSGLEKIEGDQGGRPEQTNGESLEQKPPGSKPSIRDDQRSPSPQAKKEWEEWHHKSFHGNCDINDQESKGSVSFEDRARLRQSQRRSRHRRVRTEPKENRTVSQENGPEGVAPAKGEQNGDQNENGEEKETIAEKEPVADMELKRTSEEITNNAELPMPTQDPSPSEGHQMLDKSKEASPVEQDGGSSLETSEQALLGDLSMETERTISRSEPDLSLITANMDKATESTTIMIDVQDSAVVQISNKTDGEASPLKEAETKEEEAAEAEKKNKKRKKEKSSKTGKPMVPHSSMFIFSTTNPIRRACHYVVNLRYFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYS4</accession>
    <name>A0A6I9YYS4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein C5orf42-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106554549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928725.1">
        <property type="nucleotide sequence ID" value="XM_014073250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYS4"/>
    <dbReference type="GeneID" id="106554549"/>
    <dbReference type="KEGG" id="tsr:106554549"/>
    <dbReference type="OrthoDB" id="5974632at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028236">
        <property type="entry name" value="CPLANE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14492:SF4">
        <property type="entry name" value="CILIOGENESIS AND PLANAR POLARITY EFFECTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14492">
        <property type="entry name" value="JBTS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013928725.1"/>
        </source>
    </evidence>
    <sequence length="1251" mass="142431" checksum="F7103FBCDEFA09D7" modified="2020-10-07" version="1">MKIKLEVLASTCIKQKKPWPRITWLGQEKEAVFLLDDRNMQEINLLSGKTKKKIPQLQTFLKNVIILSTSRNGAWLAGMLKTGELFLWNKDRDIIKTVPAIEESTKVAMAVQEHSLRLCLCVSGKGNKILLATPEVCVLLWESIESDATPSQISAKGQWSQIIPEASIVLPAKEEKETVVSADFIENEILGDCCLGSFAFYSGDQLVLIFLKIKWQENNASSVPCQIHWAQETCSLRNLSPSCESVKSRGALLTAFSHDGLVLAVALNQKDLQASQILFMSTKNFITTSGSLKGCGNKYPKIPSKLIRSYWISDMSWTPDTLFLVCMLKRGSLIIMTCLGELMTLVTYGCSVEFGPAEFIPLHPLITHRSQNSFLNSHDSSISEGDFMRQRFSVTCHSSLPYLIASDGYMVTVLKFSSEFSPSTYTKSLLLDSAQRLEKLHHNLITYKSEGKKQPLQSLSSLRANLLQYENQLSTFSGTPKFLQEEEGATELIEEVSLFQECNEESSDDNIFQNKSYILGSQKADFALSDEGQLEFASMFDTIHAIDDGKQLDRKKDGLLFELNHIQKNLIAAWRVATSRSIKEKDILLNFNIRCIVHFFNILQHVKLKELDHPIKNQSWIQCVLNCFHQFLTVLSWENQHRQILGHLMKLTMQTLKLILTEQQDHLFATNLLGGFSLLKMVIHYLNAKTIPQYEVLADLNGSNKVELDSIDVPLFQRHTDWNSHQKHCALCSVLTCSLPMANRTENQEKRLTIIWRYLYKHVIWYWAQLSRKMNNSNKSMTEIQIAQEIPVTKALASHIQAILQSLGENLEQLLNLKSVNGEEEFLIGSYRKSVEAWERAFQETKAKGGKRIPFLQTRYYLAILYCHLYHYNISEAQGLCDHLVYELLKRNQISVRNQDAMTDAEWIKDIHTEAALAVIQSLARFMAAYFTNEPIYIHPPHSVGILPPLHIKSDGCLRIIPLQHSRVTEAVRDNALSCTWTVEYTLDLLLISGLIPEAVWLAQKLGDWKMAVSIGVAYQLYCQSNKSLARSGNVELFLPLHLTPTNIFQEKLQSFLGQPVNNETTNQGNLRYKQLTDPIEEEDANALFSSVEQILKAAVMAEADILSETFQVLMDFAKDHSRKFCSLVPNGLYLPAPPLYCPQPTFVSEEEYADVPLRIERDCRQKISGIIQRILLLFRAAHCSFAAAQWYIARLKHARRVMQKVHIIFNSMQSDDNIWKTTFLDLIYFIGFCDSSRFYFLISTLNLEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZ17</accession>
    <name>A0A6I9YZ17_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc metalloproteinase-disintegrin-like VLAIP-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106555273</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929562.1">
        <property type="nucleotide sequence ID" value="XM_014074087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZ17"/>
    <dbReference type="GeneID" id="106555273"/>
    <dbReference type="KEGG" id="tsr:106555273"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF32">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026934538" evidence="11">
        <location>
            <begin position="21"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="13">
        <location>
            <begin position="79"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="12">
        <location>
            <begin position="283"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="190"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="232"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929562.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="48466" checksum="F320E0F6AABF39F7" modified="2020-10-07" version="1">MIQALLVAICLVVFPYQGTSIILESGNVNDYEVVYPQKVHSLPKGEVQNAELEVRKGSRQITSCQPTEFFQRRYLLAPKYIEVFIVVDNVMFRKYKSNVTAINTRVYELVNTLNMIFRILNIHLALIGIEIWSNEDQIDVQPAASVTLDSFARWRETDLLPRKRNDNAQLLTGINFDGQTIGLAYLGTICQSYNSVAIVQDYNKFTGSVASAMGHEMGHNLGIDHDTASCICQPNACIMSPVLHDVPLYDFSSCSVEQYKTFLTSNLPECILDKPLKTVVDAPAVCGNYFVEMGEECDCGSPEDCQSTCCDAATCKLKPGAQCDAEECCEQCRLKSARTVCRPAKDDCDEADLCTGRSAECPTDSFKRNGEPCQNNQGYCYNGKCPTLHKQCLALLKNSPDANVAPDECYNIYNQGRNDEGYCRRENGVKIPCGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0R7</accession>
    <name>A0A6I9Z0R7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nucleolar protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NOP14</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930172.1">
        <property type="nucleotide sequence ID" value="XM_014074697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0R7"/>
    <dbReference type="GeneID" id="106555788"/>
    <dbReference type="KEGG" id="tsr:106555788"/>
    <dbReference type="CTD" id="8602"/>
    <dbReference type="OrthoDB" id="441771at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013930172.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="29496" checksum="06479CEA21D1D71E" modified="2020-10-07" version="1">FALERQKTFEKKNVYNLNEEEDLTHYGHSLADIEKLNDIVESDSDTEEKGMLSEELTASHFGGLLSKKKPTEQQNEEGQNPKSRKELVEELIAKSKLEKKERQAQRENTLELTEKLNNDWREIQALLLPRTSKSETQSQVQEKPKPDEYDVLVRELGFEMKTQPSDRMKTEEELAKEEQEKLQQLEADRLRRMKGEEQKESRTKLTHLSADDLVDGFLLTKDDRPLLSYSVSLSSFYHPPFFKVFIFYYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z124</accession>
    <name>A0A6I9Z124_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear factor NF-kappa-B p100 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NFKB2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929540.1">
        <property type="nucleotide sequence ID" value="XM_014074065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z124"/>
    <dbReference type="GeneID" id="106555264"/>
    <dbReference type="KEGG" id="tsr:106555264"/>
    <dbReference type="CTD" id="4791"/>
    <dbReference type="OrthoDB" id="10254686at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01177">
        <property type="entry name" value="IPT_NFkappaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000046">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000004">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF21">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B P100 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="13">
        <location>
            <begin position="4"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="447"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="486"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="557"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="591"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="625"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013929540.1"/>
        </source>
    </evidence>
    <sequence length="754" mass="82868" checksum="E2A9A668C16C4FEC" modified="2020-10-07" version="1">METVEGPFVTIIEQPKQRGFRFRYGCEGPSHGGLPGASSEKGHKTYPTVKISNYTGLARIEVDLVTHTDPPQVHAHSLVGKQCNEAGNCIMMVGPKDMTAQFNNLGVLHVTKKNMMEVIRDKLQTQKMRNRIQPLTAESEQESIEQEAKELKKTMDLSIVRLRFNAYLRDSSGNFTLALNPVISDPIHDSKSPGASNLKISRMDKTAGSVRGGDEVYLLCDKVQKDDIEVRFYEDDENGWQAFGDFSPTDVHKQYAIVFRTPPYHKTKIDRPVTVFLQLKRKRGGDVSDSKQFTYYPVVEDKEEVERKRKKFLPQFPQHFGGGAPMGGAGGGAGGFGSNGAGSPWYSSGYSSYNNSIYLSGSHSMGNYQGGRGIQMPRASRPEDSEHHSSEDGSDYIELLQHAQECSSWMLMVARRSAQALLDYAVTGDPRMLLAVQRHLAASQDENGDTPLHLAIIHEQTTVIMQLVQVAVSIPNQQIINIPNHLQQTPLHLAVITHQPRVAGFLLQAGADSTLLDRYGNSVVHLAVHLDDEEMLKTLLCHLGPQILPLLEIPNYLGFFPVHLAVKCAKSASLELLVNKGANVNATEWQSGRTPLHLAVEMDNLNMAACLIKKLGADVNAQTSAGNTPLHLAAGLGSPILTKMLISAGADILCENDEPILDPSSQPNSDIELDEEEWDTELGETYGELTMQGLDYIPVSESEQPKTHETNHGRGHTPLDLTCSQKVREILLQALKQDVGEKQAGISFASNQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z230</accession>
    <name>A0A6I9Z230_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine-rich coiled-coil domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CCSER1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCSER family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930682.1">
        <property type="nucleotide sequence ID" value="XM_014075207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z230"/>
    <dbReference type="GeneID" id="106556217"/>
    <dbReference type="KEGG" id="tsr:106556217"/>
    <dbReference type="CTD" id="401145"/>
    <dbReference type="OrthoDB" id="10046062at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029627">
        <property type="entry name" value="CCSER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22461:SF1">
        <property type="entry name" value="SERINE-RICH COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22461">
        <property type="entry name" value="SERINE-RICH COILED-COIL DOMAIN-CONTAINING PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930682.1"/>
        </source>
    </evidence>
    <sequence length="557" mass="60930" checksum="D3CB37D7B754ED9D" modified="2020-10-07" version="1">MGDSTSRRSIIVSRLPIFRRSINRKHDSLPSSPTSNSTIGVHSSSPSSTNSSSGSTGKRRSLFRTPSISFHHRKGSDQKTESSCQNAGILNGAQSGHNTLQKLGLEEHAKSRSRHSVHFGSSRSKKITRSLTEDFEKGREPSTNKNVFINCISSGKNEGDDSGFAEAQSKPSVKHSSRKLLPKSFSTHYRFSKLAPQSQSVSSVQPSSCLLEIKSNIEPDPASAKSSPSECIGSSLLSTDLTTVQTPSEFLALTEDSVSEVDGLTACGNLHPETLAHNVSTSQTFAASPLSRKTNLTESISPSAETYRARNVLLAESSAFSEILDSNRKHTKVLLPELFAKQTSHLEDVNSGAKAQLKTFVLNQGEVVESSPKAQRLQRDQQKARVSEHVREIFLGIESKTTPLSSEPQSFQTQQTNDINHAKVGLPSSFSPFREGRFIEKRLRSSSEGTAGNSRMILKPKDGTPEDINCLRKQRTSSSSSKMNSLDVLNNLGSCDLDEDDLMLDLEFLEDPQHRPSVCREDSHQSIVSCSATLLTPVESAENKKKEFPQMPEVTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z290</accession>
    <name>A0A6I9Z290_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipoxygenase homology domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106555762</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930141.1">
        <property type="nucleotide sequence ID" value="XM_014074666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z290"/>
    <dbReference type="GeneID" id="106555762"/>
    <dbReference type="KEGG" id="tsr:106555762"/>
    <dbReference type="OrthoDB" id="9047512at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052970">
        <property type="entry name" value="Inner_ear_hair_cell_LOXHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901:SF3">
        <property type="entry name" value="LIPOXYGENASE HOMOLOGY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901">
        <property type="entry name" value="PROTEIN CBG12474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="194"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930141.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="32423" checksum="600D26A8C9A7DD3D" modified="2020-10-07" version="1">ISYHISIKTGDVPGASSDSKVLIKLYGEKADTKKELLLVSDNDLGNYFERSRIDIFTLDTMDIGKTSTGPCRVINYEVCTVTGDVRNAGTNAKVFLQVYGELANSTTFTVRVKTGDKKNAGTDSNVFIILYGSKDDTDHSEGWFLDWVEVDASCLGLCLKFPCGRWLDKSEDDGAIERVLFPAELQTVTYTPFVPYEITVYTSDVFGAGTDADVFIVLYGADGVSTQQKPLCQSKREQRLFFKRNSINQFIVELEDVGDILEKIRIGHDGSGINSGWHLDQVDIRRLLPNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3X6</accession>
    <name>A0A6I9Z3X6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">S-phase kinase-associated protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cyclin-A/CDK2-associated protein p45</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">F-box protein Skp2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SKP2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Following phosphorylation in response to DNA damage, mediates 'Lys-63'-linked ubiquitination of NBN, promoting ATM recruitment to DNA damage sites and DNA repair via homologous recombination.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013931798.1">
        <property type="nucleotide sequence ID" value="XM_014076323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3X6"/>
    <dbReference type="GeneID" id="106557164"/>
    <dbReference type="KEGG" id="tsr:106557164"/>
    <dbReference type="CTD" id="6502"/>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140767">
        <property type="term" value="F:enzyme-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905168">
        <property type="term" value="P:positive regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22114">
        <property type="entry name" value="F-box_FBXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000105">
        <property type="entry name" value="S-phase kinase-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46976">
        <property type="entry name" value="PROTEIN ARABIDILLO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46976:SF1">
        <property type="entry name" value="PROTEIN ARABIDILLO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="16">
        <location>
            <begin position="50"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013931798.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="42176" checksum="A9932095642DDB07" modified="2020-10-07" version="1">MGVSALKLDSENTPQDLLMCVSPPTKRQKVRETKFVIIRQPRLLQETVTGISWDTLPDELLLGIFSCLNLTDLLKVSQVCRRWHRLSLDESLWQSLDLAGKNLLPGVIGQLLPLGVIAFRCPRSCIATPLFKTSRALCVQHLDLSNCTINVIDLVAILDRCQSLQNLSLEGLLLSSGIVRSIAQNPNLVRLNLSGCSGFSADTVEVLLKSCLRLEELNLGWCDFTAEHLKIVVSHVPPKLLQLNLSGYRQNLLIPDVKTLLERCSGLVHLDLSDSVMLKPECFAYFHQLRHLEHLSLSRCYQIPPAALLELGEIATLKTLQVFGIVTANSLQLLKGTLPHIKINTSYFSPIARPTIGCKKNQEIWGIQCRLNLRNLAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4F0</accession>
    <name>A0A6I9Z4F0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leucine-rich repeat and fibronectin type-III domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LRFN2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the LRFN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930730.1">
        <property type="nucleotide sequence ID" value="XM_014075255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4F0"/>
    <dbReference type="GeneID" id="106556264"/>
    <dbReference type="KEGG" id="tsr:106556264"/>
    <dbReference type="CTD" id="57497"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05764">
        <property type="entry name" value="IgI_SALM5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000235">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000045">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000091">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000019">
        <property type="entry name" value="leucine-rich repeat and fibronectin type III domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF6">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027085969" description="Leucine-rich repeat and fibronectin type-III domain-containing protein 2" evidence="19">
        <location>
            <begin position="20"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="520"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="288"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="412"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="382"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="602"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013930730.1"/>
        </source>
    </evidence>
    <sequence length="764" mass="85107" checksum="7BC4B226CFD76E0C" modified="2020-10-07" version="1">MEKLFCSILVFGVVVAVHACPKYCVCQNLSESLGTLCPSKGLLFVPPNIDRRTVELRLGGNFIINISRQDFANMTGLVDLTLSRNTISYIQPYSFVDLESLRSLHLDSNRLPEIGEDILRGLINLQHLILNNNQLNYISDDAFEEFLFTLEDLDLSYNNLKSIPWESIMKMINLHQISLDHNLIDYITEGTFADLQKLARVDLTSNRLQKLPPDPIFARSQISPLTTTPFSPPLSLSFGGNPLHCNCELLWLRRLDRDDDMETCASPPGLKGRYFWYVREEEFVCEPPLITQHTHKFVVLEGQTATLKCKAIGDPSPIIHWVAPDDRLMGNSSRTIVYDNGTLEILITTSKDLGVFTCIAANAAGESTATIELSIVQLPHLSNGTGRTAPPKSRLSDITSSSKSNRGESKGPPDRAVLVSEVTTSSALVKWTVSKSAPRVKMYQLQYNCSDDEVLIYRMIPATNKAFVVNNLVSGTGYDLCVLALWDDTATTLTATNIVGCAQFYTKEDYPQCQSMHSQFLGGTMILIIGGIIVATLLVFIIILMVRYKICNNNPGKMANVSNVYSQTNGSQPVQNGVLPQVNPKVVVKNELMEFNCESAHSSISSSSSSMNSRDCDGYSLQSEQGTLSSKWRPPSRSKHHNIDRLMGAFASLDLKCQKKEETIDSRTSIVVRHSDKEPLLGQQESKFRSLLMLPLEGKTKRSHSFDMGDFSTSQCYNYPKKITNIWTKRSLSVNGMLLQYNDSDLTESKGTYGSSEWVMESTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4G3</accession>
    <name>A0A6I9Z4G3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Period circadian protein homolog 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106556389</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930861.1">
        <property type="nucleotide sequence ID" value="XM_014075386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4G3"/>
    <dbReference type="GeneID" id="106556389"/>
    <dbReference type="KEGG" id="tsr:106556389"/>
    <dbReference type="OrthoDB" id="7788983at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057310">
        <property type="entry name" value="PER1-3_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050760">
        <property type="entry name" value="Period_circadian_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269:SF8">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23170">
        <property type="entry name" value="bHLH_PER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Period circadian protein homolog PER 1-3 bHLH-like" evidence="4">
        <location>
            <begin position="121"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013930861.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="24379" checksum="A6B47DD9620ACE11" modified="2020-10-07" version="1">MSAGCESIQNSGGGSSSGSSSAPRRTRAPRGSDAQGQATDDMDVNSNGSSGNESPRDSHSSSHSSGNGKDSALLDTTESNKSSNSQSPSPPSSSIAYSLLSGGSEQDNPSTSGCSSEQSARVKTQKELMKALKEMKIRLPCEKRSKGKSGTLATLQYALSCVKQVQASQDYYQQWSIEDSQNCSLDMSTYTIEELENITSEYTLKNPDTFTVAVSFTTGKILYISNQAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4R1</accession>
    <name>A0A6I9Z4R1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">B-cell CLL/lymphoma 9 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">BCL9</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Binds to beta-catenin (CTNNB1), PYGO1 and PYGO2; the interaction with PYGO1 increases PYGO1 affinity to histone H3 methylated at 'Lys 4'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BCL9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932092.1">
        <property type="nucleotide sequence ID" value="XM_014076617.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932093.1">
        <property type="nucleotide sequence ID" value="XM_014076618.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106557385"/>
    <dbReference type="KEGG" id="tsr:106557385"/>
    <dbReference type="CTD" id="607"/>
    <dbReference type="OrthoDB" id="7668649at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000190">
        <property type="entry name" value="B-cell CLL/lymphoma 9, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015668">
        <property type="entry name" value="Bcl-9/Bcl-9l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024670">
        <property type="entry name" value="BCL9_beta-catenin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15185:SF5">
        <property type="entry name" value="B-CELL CLL_LYMPHOMA 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15185">
        <property type="entry name" value="BCL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11502">
        <property type="entry name" value="BCL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="B-cell lymphoma 9 beta-catenin binding" evidence="11">
        <location>
            <begin position="350"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="209"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="415"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="784"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="908"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1031"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1176"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="293"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="819"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="863"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="936"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="972"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013932092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013932093.1"/>
        </source>
    </evidence>
    <sequence length="1420" mass="149145" checksum="514D57FEFC93584C" modified="2020-10-07" version="1">MHSSNPKVRNSPSGNAQSSPKSKQEVMVRPPTVMSPSGNPQLDSKFSNQGKQGGSTGQSQPSPCDPKSGTHTPKVLPGQGGSMGLKNGAGNGSKGKGKRERSISADSFEQRDTGTPSDESEIKDLGSAEHTKSQDASHTTHSMVPSNSSVPRSSTPSHGQTTTSEPGSIQKTPSKVVYVFSTDMANKAAEAVLKGQVETIVSFHIQNISNKAERNSVSLNTQLPPLRNDTKSQIPSQLPTSQEQTPPQNAKMQQTPPIPVPAPAPAPKSASSSCPIDQDNPGLESNVMPAGSPANSTPLQAEGFRQTSTPNNRAVSPVSQGSNSSTADPKGPNQQVSGGDPPNMGENPDGLSQEQLEHRERSLQTLRDIQRMLFPDEKEFAGGQTGVSQPNSAVLDGPQKKSEGPIQAMMAQSQNLGKGPGPRTEGGAAFGSQGHRDMPFSPDEVGPPSINSQSGPIGPDHLDHMTPEQVAWLKLQQEFYEEKRRKQEQVVQQCSLQDMIVHQHGPRGLVRGPPPPYQMTPGDGWGPGGPEPFPESISMPHSLPPRGMAPHPNVPSSQMRLPGFSGMINPDIEGPNVPNTTGRPGLSGVPWPDDVPKISDGRNFPPGPGVFSGPGRGERFPNPQGLPEDIFQQHLAEKQLGLPSGISIEGIRPGMEMNRMIPPQRHMEPGNNPIFPRMPVEGPMSPSRGEFPKGIPPQMAANRELEFGIGPSSMKGDVNLNVNITSNPPMLPQKMREAGVGPEEIMKMRSGISEMLPAQQKMVPLPFGEHPQQDYGIGPRPFHPMSQGPGVGLRNHREQIGPDQRTNNRLSHMPPLPLNPSSNPNSLNTTPPVQRNLGRKPLDVSVGASQVHSPGINPLKSPTMRQVQSPMLGSPSGNLKSPQTPSQLAGILAGPTTAAAAASIKSPPVMGSAAASPVHLKSPSLPAPSPGWTSSPKPPLQSPGIPPNHKGSLAMSSPAMMGNVESGVAPPSTVSQNAPVTLSGNLPSSSPYTIPPEPTLSQNPLSIMMSRMSKFAMPSSTPLYHDAIKTVASSDDDSPPARSPNLPSINNLSGMGINPQNPRVSGPNQAGAMATLSPMGMTQPLTHSNQLPSPNAMGANIPPHGVPVGPGLMSHNPMMGHGSQDPPMVPQGRLGFPQGFPPVQSPPQQVPFPHNGPSGGQGNFPAGMSFHGEGPLGRPTSMPQSSADPALCKSGGPGGPDSFTVLGNNMPSVFSDPELQEVIRPGATGIPEFDLSRIIPSEKPSQTLQYFPRGDVPGRKQPQNVGPGYSHIQGMMAEQNPRMGLALPGMGVPGGTPDIPLGTASSMPGHNPMRPPAFLQQGMMGPHHRMMSPAQTAMPGQPTLMSNPVGMIPGKDRAAAGLYSHPGPVGSPGMMMSMQGMIAPQQNIMIPPQMRPRGMAADVGMGGFSQGPGNPGNIMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z564</accession>
    <name>A0A6I9Z564_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sn1-specific diacylglycerol lipase beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DAGLB</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931005.1">
        <property type="nucleotide sequence ID" value="XM_014075530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z564"/>
    <dbReference type="GeneID" id="106556545"/>
    <dbReference type="KEGG" id="tsr:106556545"/>
    <dbReference type="CTD" id="221955"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052214">
        <property type="entry name" value="DAG_Lipase-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792:SF2">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013931005.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="51856" checksum="F7935C94BC1C89C1" modified="2020-10-07" version="1">MPGLMLFGRRWVIGSDDFVFPGAFELFIRVTWWIGILVLYSIHKGQFNCTGGGLLNNYLLILLILLALIIAALSSITYTSMQGTISNPGPRKSLPKLLYIRLALYIPELIWAVVGTVWIADKRVKCERAMIDAIFGTVIVSWAIIIFTLVAVFIFFDPLGRKTVYLADCASRNLESSQSEQLFFNIKKRATRVWEKRIRLLCCCIMQNDDHRVAFTSIAELFRSYFSDTDLVPSDIAAGLTLLHQQQDKVENSKDPAEVLIQPPSPPEGNDLEAELENAAHYMKFAAAAYGWPYYVLLNPFTGLCKLRGFCCRNNLAQTEIIGSDYLNMHFGSILQMTGLQNRDFIHISFHNKIFEIPFFVALDHQKEAIVVAVRGTLSCEDVLTDLSADCESLTLEDVLENGLVHKGITQAANNIYRRLINDGILNQALTIAPVSCALWTLFCLSDAAAESSSPKDNKTLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7L3</accession>
    <name>A0A6I9Z7L3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18 19">Zinc finger protein 629-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18 19 20 21" type="primary">LOC106557410</name>
    </gene>
    <organism evidence="16 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932126.1">
        <property type="nucleotide sequence ID" value="XM_014076651.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932127.1">
        <property type="nucleotide sequence ID" value="XM_014076652.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932128.1">
        <property type="nucleotide sequence ID" value="XM_014076653.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932129.1">
        <property type="nucleotide sequence ID" value="XM_014076654.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932130.1">
        <property type="nucleotide sequence ID" value="XM_014076655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7L3"/>
    <dbReference type="GeneID" id="106557410"/>
    <dbReference type="KEGG" id="tsr:106557410"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000269">
        <property type="entry name" value="Zinc finger protein 287"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000342">
        <property type="entry name" value="zinc finger protein 394"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000431">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001090">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001202">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001157">
        <property type="entry name" value="Zinc finger protein 793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF241">
        <property type="entry name" value="ZINC FINGER PROTEIN 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="882"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="910"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="407"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="788"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="938"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="788"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="811"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013932126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013932127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013932128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013932129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013932130.1"/>
        </source>
    </evidence>
    <sequence length="1063" mass="119549" checksum="DFD8074025AC0AB7" modified="2020-10-07" version="1">MEQDNVPAVNVAPVSRGKGERPYPCSECGKAFSQWSKLVRHRRIHTGERPNTCTDCGKSFTQSSHLVQHRRTHTGEKPYVCQDCGKAFSWSSNLAQHQRTHTGEKPYVCRECGKAFSQSTNLIKHQRSHTGEKPYRCPECPKSFYRSSDLIQHQITHTGERPFKCEECGKGFTQSANLVKHRKIHAGEKPFRCNDCGKTFIQSSELIQHQRTHSGEKPYQCQECGKRFGHGATLVKHQRLHMGVEPYRCADCGKTFGLSSALERHRRCHSESRPYACGECGQSFSLASNLTLHARIHRGEKPYRCADCGKCFGMSSTLIRHQRIHTGEKPYACLDCGKAFVRSSHLTQHRRTHTGERPYHCEECGRRFSQSSNLITHQRIHMEERPHVCHGCGQRFALEEELQRHQQEENGKCKEKRKAKEPDRSVSSVEDLYGSSPEDDAPMQEHSENSSVTCAMCRLDSKDAGGVEQLQSRHAVHLCNICEQSFQDAETLAQHEESHTSYICTECGRSFDDAGTLACHQEDHESWICGTCGQMCRDSVALVEHEETHSLPISRPCRVKLIDRKREGQHERSRKGKENMKRSLVQEPPKVLPCINSKVAERDLMEPEEAQGAWVRLECGKSCKDVSILGSHQQSHRRSLVCSEQGHGEVNFRQEKLYQCSECEQDFGDVLALTCHQREQKCGNLDQCLECGQPVVDTSALTLRQERQLGDKVHERLQPKEASSPELDIAVHQKNQTQEPSDTALIDPQEVFAEEETSSESAFSSFNQGKPLEGQLFCCSSGLSAHQGTITDDNTSNNKPQRIPVGPSGGLPQSHSSPSLQEDESCLHPQDGCRVKSPLDASSFKEASIRARISPKSRILDKSSPVSTVMTALIQQSSTKPYKCHECEQSFAAASGLSHHQIGHTKERLLKCPECGQGFPERWALIQHQMDHRVEKHRGAHVPSSSPKENCSKDRGSPELGENFTGFSAILLQRENHISERSMLLRPKDHGADGKQTLADALETSESYFNLDLGKSPCSGGLLTQHHIRPDKGRYFVRSEPRKISRNSSLLLHHLQNHTVEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WWS8</accession>
    <name>A0A6I9WWS8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acetyl-CoA acetyltransferase, cytosolic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ACAT2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907379.1">
        <property type="nucleotide sequence ID" value="XM_014051904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WWS8"/>
    <dbReference type="GeneID" id="106537709"/>
    <dbReference type="KEGG" id="tsr:106537709"/>
    <dbReference type="CTD" id="39"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000010">
        <property type="entry name" value="Acetyl-CoA acetyltransferase (Thiolase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF107">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="8">
        <location>
            <begin position="276"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="5">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013907379.1"/>
        </source>
    </evidence>
    <sequence length="396" mass="41646" checksum="94A72F2A7BD396AD" modified="2020-10-07" version="1">MDRGDEAVVFVAAARTPVGSFNGALSTLHAHELASVVIKELLERTKIDPKEVSEVILGQVLTAGAGQNPARQASVGAGIPYEIPAWSCQMICGSGLKAIWLGAQSIMTGDSSIVVAGGMENMSKAPHVIHLRNGVKMGEASLQDTIIVDGLTDAFYCYHMGITAENVAKQWKVTRKEQDQQAVMSQNKAENAQKGGYFDKEIVPVVVPSRRGPTEVKADEHPRHGCNLETVAKLKPCFITDGTGTVTAANASGINDGAAAVILMKKSEAIKRNLIPLARIVSSAQVGIDPSVMGIAPITAIRKAVEKAGWTLDQVDLYEINEAFAALGVAITKELKLKPEKVNIEGGAVALGHALGASGCRILVTLLHSLERTGGKRGVAALCIGGGMGIAMCIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZU0</accession>
    <name>A0A6I9WZU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="17">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">PLCH1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013908499.1">
        <property type="nucleotide sequence ID" value="XM_014053024.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106538526"/>
    <dbReference type="KEGG" id="tsr:106538526"/>
    <dbReference type="CTD" id="23007"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000036">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000063">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000018">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000002">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000006">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF51">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE ETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 17" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="12 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="154"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="190"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="21">
        <location>
            <begin position="612"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="20">
        <location>
            <begin position="726"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="562"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1579"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1590"/>
            <end position="1599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013908499.1"/>
        </source>
    </evidence>
    <sequence length="1692" mass="191440" checksum="E22DAA5229DF1B62" modified="2020-10-07" version="1">MSYWNLEKRNCLQYCRHFLVDNSVFHVERCMSVMQSGTQMIKLKHGAKGLVRLFYLDEHRTCIRWRPSRKSEKAKIIIDSIYKVTEGRQSEIFHRHAEGNFDPSCCFTIYHGNHMESLDLITSNSEEARTWITGLKYLMAGISDEDSLAKRQRTHDHWVKQTFQEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLNFEEFSVFYKMMSLRRDLYLLLLSYSDKKDHLTVEDFTQFLKIEQKMNNVTTEYCLDIIRKFEVSEENKNQNVLGIEGFTNFMCSPACDVFNPLHSEVHQDMEQPLCNYFIASSHNTYLTGDQLLSQSKVEMYARVLQDGCRCLEVDCWDGPDGEPVVHHGYTLTSKIPFKDVIDVINKNAFTKNEFPIILSIENHCNVEQQKKIAQYLKEIFGDKLDLTSVNIGDLKQLPSPQSLKGKILVKGKKLPYSLGADAEEGEVSDEDSADEIEDDCKLNFSYSNGTTDHQIESFIRKKLESLLKESQIRDKEDTDSFTVRALLKATHEGLNVNFKPHSGLKDKKSHNKLMGTFGKHKKAAKSKSKSHCGSDDEDSQQNVSGKESGQLYSRLARRRKTMKLCRDLSDLVVYTNSVAAQDIIDDGSVGNVLSFSETRAHQVIQQKSEQFLLYNQQQLTRVYPSAYRIDSSNFNPVPYWNVGCQLVALNYQSEGRMMQLNQAKFRMNGNCGYILKPQQMCKGTFNPYSGDPLPAFPKKQLILKIISGQQLPKPPDSMLGDRGEQIIDPFVEVEIIGLPADCCKDQTRVVDDNGFNPVWEETLTFTIHMPEIALVRFLVWDHDPIGRDFVGQRTVAFSSLMPGYRHVYLEGLTEASIFVHVTINEIFGKWSPLILNPSYTIMHFLGATKNKQLIGLRGLFNRNSKHNSVENSTNYIRKRSLGDRILRRTASAPAKARKKNKMCFQETTDLKESVSDMLDLKEKEGGFRQTSRSLQLRPISMPVGKYLLAGLPPPDQDASRVVEVIKSESTSAESRNDASLDCTNAKKTITSSEKNPPFTYLLKKDVAKSGTCDTLCPDEKGESLNFAIGIAETYFSTEYWKSNLPNEFVHVKEDLERKKQEDIGRMDRKTLEKTELCKERRKIADEKANMESLPILTTTITNVCSFNGTPAIPDSDISCLINEVSLAGERERDNSISKLIEQVDVTSDQTDLTLVSSPSDINKEKNNLKSLHLAPCTSLDEPSIILDDKSLNLKSAGSACSTGPRTENTIISTPKTTGCSVYTIIHEASLSPVSQSAFTDRSVCKDTAKDAEDSCLNQGAKHTSCISPIPMQKANPKKKCGTSWEFLGNSNSYTSDIQNTIVVPVTCANSTGSSLMEIDGELQDLLITAFEYKIEDMSQVASPLKLKHSQDTLYYNKTICEPPKSMDFPSENLAEDLKFNNDKNQYFPYPRQQRFELLYNNCLQISDAQNELLNIPQSASINAALKPQNVLPSPFPGAQKQQSPCKSKSLGDLTSEDISCNFESKYKYISRGFITSGMRDNMVATLKAKKPSTTDILTEQLRKLVSFEQEESCQSFCSKQNDDCPKMLVRKLSSRSQSRVRNIASRAKERQEANKQKPSNVSSNVTDIVLRNKPPVPPHIINRHSTGSYIASYLDNFNTDELERRGVPEGACSSLHLGYSDRFCTDDSLLETKPNENDKPEIYFLLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X1M8</accession>
    <name>A0A6I9X1M8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PHD finger protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PHF6</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909159.1">
        <property type="nucleotide sequence ID" value="XM_014053684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X1M8"/>
    <dbReference type="GeneID" id="106539015"/>
    <dbReference type="KEGG" id="tsr:106539015"/>
    <dbReference type="CTD" id="84295"/>
    <dbReference type="OrthoDB" id="2384350at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15710">
        <property type="entry name" value="ePHD1_PHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15711">
        <property type="entry name" value="ePHD2_PHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000167">
        <property type="entry name" value="PHD finger protein 6 X-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051188">
        <property type="entry name" value="PHD-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420:SF15">
        <property type="entry name" value="PHD FINGER PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="14"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="207"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="328"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013909159.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="40805" checksum="707B6F294B258FC8" modified="2020-10-07" version="1">MSGTVGQKKGPRRQRKCGFCRSNKEDECGQLLLSENQKVAAHHKCMLFSSALVSSHTDNESLGGFSIEDVQKEMKRGTKLKCSLCHCPGATIGCDVKTCHKTYHYYCALHDQAQIREKPSQGIYMILCRKHKKAPHNSEADLEEGLNEHELDPSPPKKRGRKGRPRKTSLKGQSEDARSTSSHGTDDVESSSYRDRSPHRSSPNDTKPKCGFCHAGEEENEARGKLHIFNAKKAAAHYKCMLFSSGTVQLTTTSRAEFGDFDIKTVLQEIKRGKRMKCTLCSQSGATIGCEIKACIKTYHYHCGVQDKAKYIENMSRGIYKLYCKNHSGNDERDEEDEERENKSRAKSVADHSAIPQQQQLNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X2X1</accession>
    <name>A0A6I9X2X1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Squalene monooxygenase</fullName>
            <ecNumber evidence="7">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106538261</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="7"/>
    <dbReference type="RefSeq" id="XP_013908187.1">
        <property type="nucleotide sequence ID" value="XM_014052712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X2X1"/>
    <dbReference type="GeneID" id="106538261"/>
    <dbReference type="KEGG" id="tsr:106538261"/>
    <dbReference type="OrthoDB" id="1678617at2759"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0274">FAD</keyword>
    <keyword evidence="3 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="9">
        <location>
            <begin position="174"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013908187.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="24331" checksum="CFF037813F618CC3" modified="2020-10-07" version="1">MWTFLGIASFIYVYKKCGDLLSYANKEVLISTMVFFSLGLMLSYWYRSWGPQPQNKQKPHCGMFSNFLAALPFVGFFWTKSHTDSLEKLQTKTRKASHELYCKIKIEEAVEGIDAHTINGYIVHNLENKSEVEIPYPCTAEHRVQNGKAFHHGRFIMGLRRAAISEPNGHKGNDAVMSCFLCLTDSLHKLRRACFFYFKLGGKCVSGPVGLLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6I7</accession>
    <name>A0A6I9X6I7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zona pellucida sperm-binding protein 3 receptor-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106540487</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911074.1">
        <property type="nucleotide sequence ID" value="XM_014055599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6I7"/>
    <dbReference type="GeneID" id="106540487"/>
    <dbReference type="KEGG" id="tsr:106540487"/>
    <dbReference type="OrthoDB" id="6127264at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000014">
        <property type="entry name" value="Membrane cofactor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026856127" evidence="6">
        <location>
            <begin position="29"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="28"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="93"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="154"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="220"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="156"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013911074.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="30632" checksum="BA3C2D0F74BA9E03" modified="2020-10-07" version="1">MAAFPCSQTGLLAWMLLLLLLSSSEVQSECQTPVLPPNSSPRGGGDLAQSYPIGTVLRLQCDPGYTPKSGTIRSMTCLDTNKWSELPTLCERRRCTTPSIDFGNMESTDGLLLGDKVTFSCNEGYILIGSPTLRCVLRFGKVDWDRSLPYCKSIFCTSPPLIANGDYEGGSSGEYHVFSAVTYWCKAGYSLIGQSTIACKVAENGRDGIWDSSPPECKKVKCLRPNIPNGRMPYGSRETYTYQHSIFIECSPGFTLVGSSTIYCDANSEWNPSVPQCVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X7L6</accession>
    <name>A0A6I9X7L6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Transcription factor GATA-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GATA6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909832.1">
        <property type="nucleotide sequence ID" value="XM_014054357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X7L6"/>
    <dbReference type="GeneID" id="106539548"/>
    <dbReference type="KEGG" id="tsr:106539548"/>
    <dbReference type="CTD" id="2627"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000001">
        <property type="entry name" value="GATA transcription factor (GATAd)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000032">
        <property type="entry name" value="Transcription factor GATA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008013">
        <property type="entry name" value="GATA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF23">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05349">
        <property type="entry name" value="GATA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="13">
        <location>
            <begin position="310"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="13">
        <location>
            <begin position="364"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="187"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013909832.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="52109" checksum="D720EFD084095D83" modified="2020-10-07" version="1">MALAAGGWCLLKRCGPDDSPCPLAPRELLGPSPSPSSSSGCSPSGSGRPHNARSFKQEPGGALSTSPQIGLAEGRALLGPFVSLAAAAAPSSLPSWEDLLLFADLDQANKLIWSSRSSTKLSPDPAPPPPIVEDMYHHPHAHPHSQSLQASTYDGASPGSGGGGFVPSTAGSPVYVPTTRVGSMLPYLQNPPGVGQAGGSSAWPSQGAPESPSSSYGASAGGCAHPASGRFSYPAGSPPASNGAPREPAAYSPREQFRPLNGSYYNPYVGAQFSPPGWGSPFDNSLLHCLPSRPTTLAVRPSSADLLDDLNESRECVNCGSIQTPLWRRDGTGNYLCNACGLYTKMNGLSRPLIKPQKRVPSSRRLGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNIAKTKSCSGKYIQRFLLQAVSSVMSNRGESTSPETSTLKYSGQEGLYSGVSLTSTAEVTASVRQDSSWCALALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8Y5</accession>
    <name>A0A6I9X8Y5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">HIV Tat-specific factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">HTATSF1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HTATSF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910212.1">
        <property type="nucleotide sequence ID" value="XM_014054737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8Y5"/>
    <dbReference type="GeneID" id="106539839"/>
    <dbReference type="KEGG" id="tsr:106539839"/>
    <dbReference type="CTD" id="27336"/>
    <dbReference type="OrthoDB" id="10258585at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12282">
        <property type="entry name" value="RRM2_TatSF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000105">
        <property type="entry name" value="HIV Tat-specific factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034393">
        <property type="entry name" value="TatSF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15608:SF0">
        <property type="entry name" value="HIV TAT-SPECIFIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15608">
        <property type="entry name" value="SPLICING FACTOR U2AF-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="26"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013910212.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="17006" checksum="58E8CC94FFFA287E" modified="2020-10-07" version="1">MLLHFIRLLDWRPEKKEGSVRLRYERVIIIRNMFHPKDFEEDPLVLNEIREDLRSECEKFGQVKKVLIFDRHPDGVASVSFKDSEEADVCIQALNGRWFGGRQLSVETWDGVTDYQIEETSREREERLKGWQEFLGDPNQELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X951</accession>
    <name>A0A6I9X951_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neurochondrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NCDN</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the neurochondrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013910277.1">
        <property type="nucleotide sequence ID" value="XM_014054802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X951"/>
    <dbReference type="GeneID" id="106539884"/>
    <dbReference type="KEGG" id="tsr:106539884"/>
    <dbReference type="CTD" id="23154"/>
    <dbReference type="OrthoDB" id="8186546at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048168">
        <property type="term" value="P:regulation of neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008709">
        <property type="entry name" value="Neurochondrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13109">
        <property type="entry name" value="NEUROCHONDRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13109:SF7">
        <property type="entry name" value="NEUROCHONDRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05536">
        <property type="entry name" value="Neurochondrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013910277.1"/>
        </source>
    </evidence>
    <sequence length="614" mass="67133" checksum="1EEB39A7154BFB75" modified="2020-10-07" version="1">MASNSGIVVDDGESSKNCTLERCLNVFRDASNDSEQFAALLLVTKAIKAGEINSKARRRIFDAVGFTFPNRLLTSKDPPEGCPQHTFQALGLTLLACFCTDPDLARHPQMLNKIPILSDVVSAKTRDASYSAMIDDAYQCLRAILATPKGPRELINRGGLSALCQAYVNHSYGYDQALQLLVGLLTSAETKCWQRATTDLMGVLSVLSAEFQKAEDMTKFQLCEVLARFVPPLLPPGSECLLPLYRGLSSILTSKLSASQRDPALKLSACLSQACSSAWIPTGSSGSKFFALLVNLACVEVRLTLEELDLTEVDKKRELMAACYILMELAIVECTREKGSLLQETQKVQLVGILEEALGAVIHYLRQGLVFPPSRFLLPGLSHLTAEDRPREILISEGAPVLLCQYFLHRWEIFVSNTESQSASKVIEASLQTSCEIFLNFVVTAPDLIREKHCPGSISCACLLHFPSPGLQGTPEADSFFGAAIRFLGDAYVAQASTEGGIVTLGVSPTYKAAWPAIRELWFLGMQAFASCVPLFLWLPQDVLRSGWLQDLLALLGRVIPSSVDMEVARAYQGVLVELARASQPCLERIQQHRGLELASLYGMAALEQCLSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XA02</accession>
    <name>A0A6I9XA02_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Eukaryotic translation initiation factor 4E transporter isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">EIF4ENIF1</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911149.1">
        <property type="nucleotide sequence ID" value="XM_014055674.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911150.1">
        <property type="nucleotide sequence ID" value="XM_014055675.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013911151.1">
        <property type="nucleotide sequence ID" value="XM_014055676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XA02"/>
    <dbReference type="GeneID" id="106540526"/>
    <dbReference type="KEGG" id="tsr:106540526"/>
    <dbReference type="CTD" id="56478"/>
    <dbReference type="OrthoDB" id="8916892at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018862">
        <property type="entry name" value="eIF4E-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12269">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12269:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10477">
        <property type="entry name" value="EIF4E-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="653"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="714"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="910"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="686"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="714"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="757"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="911"/>
            <end position="925"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013911149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013911150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911151.1"/>
        </source>
    </evidence>
    <sequence length="988" mass="108669" checksum="C869468327E77743" modified="2020-10-07" version="1">MDKRGGLAETVNGDALLNMNIISTMKFPHRYTKEELLDIKEHPQSKQRPSCLSEKYDSDGVWDPEKWHASLYPNSGRTSPVESLKKELDSDRPALIRRIPDPRERVKEDELDIVLSPQRRSFGGGCHVTAAACTRRSGSPLEKESDCVRVIGGRRIGSGRIISARNFDKDHRSGEKDSRDVRERDRDREYKDKRFRRDYGDSKRLFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEEHKGRKRTRRRTASLKEGIECNGGVTEEEELRTTRGQETAADQEVPKEMVLPESAPGEFDFNEFFNLDKSVPGLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLRSTPHEELERLAGLEQGILSPGQNSGNYFAPIPLEDHSENKVDILEMLQKAKVDLKPLLSSLSANKEKLRESTHSGVVLSVEEVEAGLKGLKMDQQGKTAAPLMAERMEESLNVADSRHFKDGDMSAFNKLVSTMKASGTLPSQPKVNQTSETPLISPSEMPGQAATKNILQEILGPSPSCRQTSSNVLSTLMGGLEPVPSLLSQRASSPPQPPISQMFPTRAASADYLRHRIPSPVGFGPGAQQLLTDPFQGIRKPVSPAAATAAQMNPLELQQAALEGLSLPHDLAMQVPNFYQPGFGKLQMDKNRDGFRNRQPRMTQSPASLGLRGNTSSPPTTAASITSMLSPSFTPTSVIRKMYESKDKSKDEPHAAKLKSSEREEGPRTTEESLLLSRSTDHVDQENSPSVAPKLTSLQRSTCPTPLSQANRCTKEQDYRPKSSGRKTPTMASPVPGNTFLRPIHQVPLVPHVPMVRPAHQLPPGLVQRMLAQGIHPQHLPPLLQAGMIPPGVDLSHLQGVPAPLLGQPCYPLTTAGHPLLNPRSATPLQLAMMQQQLQRSGAGTQGSAVGVQTTPPPVSSRTGLPHMHSQLDHRATAQRSSSPVGLAKWFGSDVLQQPLPSMPSKVISVDELEYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XA82</accession>
    <name>A0A6I9XA82_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Centromere protein S</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106541549</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family. CENP-S/MHF1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the somatostatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912509.1">
        <property type="nucleotide sequence ID" value="XM_014057034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XA82"/>
    <dbReference type="GeneID" id="106541549"/>
    <dbReference type="KEGG" id="tsr:106541549"/>
    <dbReference type="OrthoDB" id="1872155at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22919">
        <property type="entry name" value="HFD_CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029003">
        <property type="entry name" value="CENP-S/Mhf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018142">
        <property type="entry name" value="Somatostatin/Cortistatin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980:SF0">
        <property type="entry name" value="CENTROMERE PROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980">
        <property type="entry name" value="CORTISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15630">
        <property type="entry name" value="CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03002">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Somatostatin/Cortistatin C-terminal" evidence="12">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="107"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013912509.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17386" checksum="EFEA9A3DF75652E9" modified="2020-10-07" version="1">MEAEAQRLKAAFHYTVARLCEDVAEDKNIQFSRQTIAAISEITFRQCEMFAKDLEMFAKHGKRSTVNVEDVKLLARRSNSLYFQAPADLEKKELLVLLSGLTERASSHMNTSHLSRQEKIGAGPRGHRVPAPQPPTREKPSCKNFFWKTFSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBU2</accession>
    <name>A0A6I9XBU2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Apical endosomal glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MAMDC4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913049.1">
        <property type="nucleotide sequence ID" value="XM_014057574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBU2"/>
    <dbReference type="GeneID" id="106541981"/>
    <dbReference type="KEGG" id="tsr:106541981"/>
    <dbReference type="CTD" id="158056"/>
    <dbReference type="OrthoDB" id="8847287at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051560">
        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282">
        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF101">
        <property type="entry name" value="MAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026872740" evidence="5">
        <location>
            <begin position="27"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1102"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="6">
        <location>
            <begin position="65"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="6">
        <location>
            <begin position="274"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="6">
        <location>
            <begin position="416"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="6">
        <location>
            <begin position="590"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="6">
        <location>
            <begin position="752"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="6">
        <location>
            <begin position="916"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1076"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913049.1"/>
        </source>
    </evidence>
    <sequence length="1164" mass="125075" checksum="7853E383E47EF4C1" modified="2020-10-07" version="1">MPGSWGLLSFLLLQMLMVGLPFYVQAVFVNRCGSSRVCDFVCDCWDCSDENQCGYQTASARGAPSTCSFEGTTCGWEDISTTAYRWDPAQASISPWGLEPPFDHTLGTDSGWYMAVTKQRAKPSATARLRSPLLRGAAASCEIRAWYLLWGSGLNETQQPLLTLELSQGNHTVRLWQSPAGGTHSWQELVAYTGHIPGNFQVTFSSTQGFSSSAQLALDDVEFRNCGLPPPQACGAEAFRCQQGSCIAIDRVCDGTADCRGGEDEAQEECASSTVCSFEEDWCQWTPNTTLPWLRNSSLHLASPSLGPWRDHSTNSREGFFVYVEAQKPELHEGRAWLSSPMLASDANQSCYVSDLSHSWRGSSLAEGLGGFPLLVVIAAPLPLSLFLYKILLPSSPCPPGQFACDQGKQCLDLQLLCNFKAECKDASDEQQCGGTDFAKGPGGWMDISVGRLRWTTSRLSSVGPVLSLQGAPGQMFSMARAATPVLGPSGLGCVLQMDFTAGPEGFLALAIADGSLGTRWWAWFAKSNGTASWAEATVRLGARKRPFQLELLARADLDGPWGALPLAVSNISFVNCSAEAPASAPQAGLSCNFETGWCSWSLEQNDGFEWRLSRSRRGAADHTTGTGSFLLAEPGGAWNPGRRAQLISAPQVAAAASSATVEASCLSFWYRMEGPQIGTLALKVKAAGEPERVLWTRRGSHGAVWHQASSSISPKPGQNYQVIFEALRDGFLGSVALDDLTVTPGDCPAQRHCSFEADECSFSAAKQQAWQRQSGAGGGGPPVDHTLGELRGHYLILRTGADRLPLGQVTVLRSRAFPPLLRTHCVSFWYHLNGSEPGSLTAYVKEEEGKEEGAEAGMLSPDPTQGAAWRYGSFVAKVRRRWQVAFTVTGAGGEPTSYIALDDISVKEGNCSEPGSCDFESGPCGWSKPHGDGYSWDWKEAATPLRSPSPEEDHTLGTRAGHYAYVDLAVLSLGKTAARLASEPLAPTTGSCLSFHYHMDFLGHSSPAELRVRLTGAQGERVIWSARGHQSRTWMNRTLLVTSPVEFQIVLEASSGAWESSGVIALDDLHYTAGPDCASPQAGPAEESSSSSSSSLPVSTIAGVAGGVTVLLLLIAAAYCLWKRKGSGERMEEEDGNGPSFDNITFRDDRIILTQMAPDPATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDN0</accession>
    <name>A0A6I9XDN0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Probable ATP-dependent DNA helicase HFM1</fullName>
            <ecNumber evidence="13">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">DNA 3'-5' helicase HFM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">HFM1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Required for crossover formation and complete synapsis of homologous chromosomes during meiosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013911972.1">
        <property type="nucleotide sequence ID" value="XM_014056497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDN0"/>
    <dbReference type="GeneID" id="106541145"/>
    <dbReference type="KEGG" id="tsr:106541145"/>
    <dbReference type="CTD" id="164045"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18023">
        <property type="entry name" value="DEXHc_HFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001076">
        <property type="entry name" value="ATP-dependent DNA helicase MER3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000950">
        <property type="entry name" value="probable ATP-dependent DNA helicase HFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000006">
        <property type="entry name" value="probable ATP-dependent DNA helicase HFM1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052247">
        <property type="entry name" value="Meiotic_Crossover_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835:SF3">
        <property type="entry name" value="HELICASE FOR MEIOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835">
        <property type="entry name" value="HFM1, ATP DEPENDENT DNA HELICASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="103"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="333"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013911972.1"/>
        </source>
    </evidence>
    <sequence length="1081" mass="122849" checksum="26FED302BBCE28DF" modified="2020-10-07" version="1">MDTYCNIINNKEMKKDLWKNDMQIAQNSDFNYLSINNTFSASANTKEIKKAPSFPRISDISSTEVISKNDSVVLRPVTEIPTQFRNIFKEFPYFNFIQSKALDDILYTDRNFVICAPTGSGKTVMFELAVTRLLIKVPTPWLNIKIVYMAPIKALCSQRFDDWKEKFGPIGLICKELTGDTAVDDLFEIQHAHIILTTPEKWDSMTRKWRDNSLVQLVRLILIDEVHVVKDESRGATLEVVVSRMKTIQSSFLQSSDNPSTVFPMRFVAVSATIPNAEDIAEWLSDGKRPAVCLKIDERHRPVKLHKVVLGFPCSSNQTEFKFDLTLNYKVASVIQTYSEQKPTLVFCATRKGVQQAASVLAKDAKFIMTIEQKQRLQRSANLIKEAKLRDLLIYGVAYHHAGVEVSDRKIIEAAFNMGDIPVLFTTSTLAMGVNLPAHLVVIKSTMHYAGGMFQEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTRDKYVHMLNGADTIESSLHKHLIEHLNAEIVLHTITDVNIALEWVRSTFLYIRALKNPAYYGFSAELDRNGIEAKLQELCLKNLNDLSSLDLIKMDDNLYFKTTETGRQMAWYYIAFDTVKKCFMINGTATLSQLVELISGCSEFSDIKLRTNEKKVLNSLNKDKKWMNIRYPMEGKIKTREMKVNCLIQAQLGCIPIQDFTLTQDIGKIFRNGIRLTKWLSEFLATHQNNFIVLLNALILAKCFRCRLWENSLYVSKQLEKIGMSLSNAMMNAGLTSFKKIEDTNARELELNGNRKCNHHCKNKETCGHDCCKIGVSEKSNMKNDSAFSSYLTDLRNRNAESAIPPVKRLKMQMSNKSQKVNLKQFSYIPKASLPGLPRLEHGQHPRSPLPEDIYSSNVCQTFQLEPEVRKINGFFPIKQSDEKNNLTMSSHESLEESSMSNLTDADKSISLFSETLNVNFELGNEIWSDYNDGNHSNANMQKLKDPEKTKPDICLQKSYSPSLTPPSSSKLLLECGNQDIFRLQEEYNPPEKLKMAQLTTTSETTLTSYQVAEKGDFFITNRTMTKGADLRHQLDDNGDIKPFLGIFDGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDY1</accession>
    <name>A0A6I9XDY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fucosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">FUT7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56076"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:136545"/>
            <dbReference type="ChEBI" id="CHEBI:139509"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:56077"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">Golgi stack membrane</location>
            <topology evidence="12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013913834.1">
        <property type="nucleotide sequence ID" value="XM_014058359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDY1"/>
    <dbReference type="GeneID" id="106542581"/>
    <dbReference type="KEGG" id="tsr:106542581"/>
    <dbReference type="CTD" id="2529"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000001">
        <property type="entry name" value="alpha-(1,3)-fucosyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF12">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="14">
        <location>
            <begin position="42"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="13">
        <location>
            <begin position="163"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013913834.1"/>
        </source>
    </evidence>
    <sequence length="339" mass="39779" checksum="9A2157443E747150" modified="2020-10-07" version="1">MAPVAKFWAGASLLGIVLFWNVPLLFKLSDDPPRFTRHNGTLSLLIWSWPFGAATDLTGDVCSRLYGITGCQLTTNRHLFNQVDVVVFHHWELQYERRDHLLSKAHPGQKWVWASLESPSNTKALEGWTGKEWNWVLSYRRDADLFFPYGELVPHSLEVVEIPEKTGLVCWVISNYHASQARAVMYHELSRHLRIDVFGKANRKLLCPDCLLPTISKYRFYLAFENSIHRDYITEKLWKNAFLAGSVPIVLGPSRANYEQFIPANSFIHVDDFSSAKDLATFLTTMNESHYRNFFAWKRNYTVRLHQDWRERFCSICQQYPHLPPNKLYPDLKRWYWDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFT8</accession>
    <name>A0A6I9XFT8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial import receptor subunit TOM5 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TOMM5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913031.1">
        <property type="nucleotide sequence ID" value="XM_014057556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFT8"/>
    <dbReference type="GeneID" id="106541961"/>
    <dbReference type="KEGG" id="tsr:106541961"/>
    <dbReference type="CTD" id="401505"/>
    <dbReference type="OrthoDB" id="8893106at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019603">
        <property type="entry name" value="Tom5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029179">
        <property type="entry name" value="TOMM5_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28436">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28436:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10642">
        <property type="entry name" value="Tom5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013913031.1"/>
        </source>
    </evidence>
    <sequence length="51" mass="5989" checksum="85E4DC293DD0621A" modified="2020-10-07" version="1">MFRLEGLGPKLDPEELKRKMREDVLSSIRNFLIYVAVLRITPFVLKKLDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGK7</accession>
    <name>A0A6I9XGK7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106539806</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910173.1">
        <property type="nucleotide sequence ID" value="XM_014054698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGK7"/>
    <dbReference type="GeneID" id="106539806"/>
    <dbReference type="KEGG" id="tsr:106539806"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="7">
        <location>
            <begin position="34"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013910173.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="33486" checksum="95E9DB99EC4A7DBE" modified="2020-10-07" version="1">MHVCSECPEDQHPSNERDSCLAKKISFLSYEEPIGIGLLVLALIFSFCTVSVLAIFLKYHHTPIVKANNRNLTYILLISLLLCFLCSLQFLIIPGNIICLLRQVSFGIIFSVAVSSVLAKSITVVLAFMATKPGSKVRKWVGKRLAISIVISCSLIQAGICIVWMFIFPPFPDIDTHSKMEEMILQCKEGSGIMFYCVLGYMGFLAAASFTVAFFSRKLPSSFNEAKCLTFSMLIFCSVWLSFIPSYLSTKGKYMVAVEVFSILASSIGLLGCIYFPKCYIIIVRPELNKREQLIKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHV2</accession>
    <name>A0A6I9XHV2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chromobox protein homolog 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CBX8</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913472.1">
        <property type="nucleotide sequence ID" value="XM_014057997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHV2"/>
    <dbReference type="GeneID" id="106542310"/>
    <dbReference type="KEGG" id="tsr:106542310"/>
    <dbReference type="CTD" id="57332"/>
    <dbReference type="OrthoDB" id="1918685at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18627">
        <property type="entry name" value="CD_polycomb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000006">
        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033773">
        <property type="entry name" value="CBX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF1">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17218">
        <property type="entry name" value="CBX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="7">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="92"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013913472.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="40388" checksum="54AEB1BD5C221147" modified="2020-10-07" version="1">MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPKKRGPKPKTFLLKAQAKAKAKTYEFRSDSARGLRVPYPGRSPQELGSTSRAREGLRQIPLPPQSTSTTSTPSMPRMEPIQENRVGIEEDRSEAAFKKRGPKPRKDLPEMTEVASKRKSIEPGDKMVVDYLKARKLEETDNSGTAKFSSGHSVIQLARRREPELPSVVPNPRAETGMKLGAPETYPPRLTKHRADFLDSKGQSGLDLGASKILHSSANQGSLYRDGIGTQAGRPSLIARIPVSRILGDPEEEPWSPSLNNLEKVVVTDVTSNFLTVTIKESSTDQGFFKEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHY5</accession>
    <name>A0A6I9XHY5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106543795</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915349.1">
        <property type="nucleotide sequence ID" value="XM_014059874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHY5"/>
    <dbReference type="GeneID" id="106543795"/>
    <dbReference type="KEGG" id="tsr:106543795"/>
    <dbReference type="OrthoDB" id="9440489at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0552">Olfaction</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="42"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013915349.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="34476" checksum="3FD801D8046EA48C" modified="2020-10-07" version="1">MAQQNNYTMVTEFILLGFTDEPNMQMVLFFLFLAIYLFTVTGSLGLIVLIRTDSHFHTPMYFFLLHLSFTDLCYVSVIAPKMLVNFLVDHKSISYTGCVVQLWLFTAFVTTECFLLAVMAIDRYMAICNPLLYPVFMSQEICNCLVAGSYAAGVANSVLQTACIFRLSYCGCNILKHFFCDIPKLFQLSCSESHLSESLSVFASGIVAMTSISVIIISYLCILSAILKINSMKGKRKAFSTCASHLTSVALLYGTGTFTYIHPNLKSGQDMDMVISIMYTIIIPMLNPLIYSLRNKEVKEAIRKVTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XIL5</accession>
    <name>A0A6I9XIL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Serine/threonine-protein kinase TNNI3K</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Cardiac ankyrin repeat kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">TNNI3-interacting kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC106540234</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">May play a role in cardiac physiology.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013910763.1">
        <property type="nucleotide sequence ID" value="XM_014055288.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106540234"/>
    <dbReference type="KEGG" id="tsr:106540234"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903779">
        <property type="term" value="P:regulation of cardiac conduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002027">
        <property type="term" value="P:regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14064">
        <property type="entry name" value="PKc_TNNI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000259">
        <property type="entry name" value="Serine/threonine-protein kinase TNNI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000077">
        <property type="entry name" value="Serine/threonine-protein kinase TNNI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000089">
        <property type="entry name" value="serine/threonine-protein kinase TNNI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF62">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TNNI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="13 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="25">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="25">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="25">
        <location>
            <begin position="169"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="25">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="25">
        <location>
            <begin position="269"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="25">
        <location>
            <begin position="339"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="463"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00080194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013910763.1"/>
        </source>
    </evidence>
    <sequence length="836" mass="92891" checksum="99E1ACBE30AB17B1" modified="2020-10-07" version="1">MGNYKSRPTQTCTDEWKKKISEAYAVIIERLEDDLLIKEKELTELRHVFSSDEAFSKVNLNYRTENGLSLLHLSCICNGHKTHIRTLMLKGLRPSRLTRNGFTALHLAAYKDNAELLTALLHSGADVQQVGYGALTALHIATIAGHHEAVDILLQHGAYVNVQDAVFFTPLHIAAYYGHEQVTRLLLKCGADVNVSGEVGDRPLHLASAKGFLNVAKLLMEEGTKADVNAQDNEDHVPLHFCSRFGHQDIVKFLLQSNFEVQSHVVNIYGDTPLHLACYNGKFEVVKELIQLSGTESLTKENIFSETAFHSACTYGKNIELVKFLLDQNVMSLNHQGRDGHTGLHSACYHGHIRLVQFLLDNGADMNLVASDASRSSGEKDEQTCLMWAYEKGHDAIVTLLKHYKRPQDESPCNEYSLPGGDGSYVSVPSPLGKIKSMTKEKADVLLLRAGLPSHFHLQLSDLEFHEIIGSGSFGKVYKGRCRNKIVAIKRYRANTYCSKSDVDMFCREVSILCRLNHPCVIQFVGACLDDPSQFAIVTQYISGGSLFSLLHEQKRNLDLQSKLIIAVDVAKGMEYLHNLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDEDNMTKQPGNLRWMAPEVFTQCTRYTIKADVFSYALCLWELLTGEIPFAHLKPAAAAADMAYHHIRPPIGYSIPKTISSLLMRGWNACPEGCEDFGVVINKYXXCPLPYIQLMSPASSNSSGSLSPSSSSDCLLTRGCPGRSHVAALRSRFELEYALNSRAHGTWSQSTGQQSSQGLSLEEMRRNFQCSPIDQNGYVSDPMSTLRFPSCSNSGSFEDSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ32</accession>
    <name>A0A6I9XJ32_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interferon regulatory factor 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106542752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914046.1">
        <property type="nucleotide sequence ID" value="XM_014058571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJ32"/>
    <dbReference type="GeneID" id="106542752"/>
    <dbReference type="KEGG" id="tsr:106542752"/>
    <dbReference type="OrthoDB" id="5958224at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019471">
        <property type="entry name" value="Interferon_reg_factor-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF26">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10401">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01243">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon regulatory factor-3" evidence="2">
        <location>
            <begin position="102"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914046.1"/>
        </source>
    </evidence>
    <sequence length="277" mass="30590" checksum="D6BB880D2F59D2C2" modified="2020-10-07" version="1">MGKKTKMQKTKRARMEDARSLKEEASGSREEIRATLALQRAEALAPEAMVSHVANAFNPPNCQGGEVSPQAAEPAPDVNQITLNLRPDPKPVVRVATGPTEEFSLHMSISYLGELLCEHLLPEGEFLITSAQALPNGPVNCLKRFILPPPDKIADAPKQKIVQRLLDDLVKGIMVASNHEGIFIQCRGKVCVSWSGFLAPEGQIKLENDTLQQLFKPREFRSALEQHRQGLAPFPDPRVILYVGNEAGKNGHVDSQPIVIQMEQTFAIRLRSSDYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLI7</accession>
    <name>A0A6I9XLI7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">EMILIN-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EMILIN3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914772.1">
        <property type="nucleotide sequence ID" value="XM_014059297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLI7"/>
    <dbReference type="GeneID" id="106543287"/>
    <dbReference type="KEGG" id="tsr:106543287"/>
    <dbReference type="CTD" id="90187"/>
    <dbReference type="OrthoDB" id="10266508at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026734940" evidence="2">
        <location>
            <begin position="32"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="509"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013914772.1"/>
        </source>
    </evidence>
    <sequence length="783" mass="87916" checksum="3B4574CD978AA1B2" modified="2020-10-07" version="1">MTRAKGFSRAVAFGASVSLLCAGTLVCLTEAKGTYYRHPLPISYGNRYNLYTSGSSPQLTPEKPLARHKTYCAYVVQRNVTCTLQDGVESYVKAEYHKCSWGPKCPGKVVVVKLAKLKNEGEGVFNPPLLCGALLLRSRWKHLPRHFWVEKYGRKIPNVFGERLDHLEEEVRRLSQSYDTLYNMVSGLGDHLRLAIQEDTSKMIGSLMNSPTVPDSTMGFGIIPEGIVDAADKAELSPYPPVGEILGKVTEMSDSLKIKTNLLEEVHGMVLDHDGQIKNLMESARPSPLTSIDMLEEYIDNRLSNLRLELLDGFEKKLINIQSTCDYQIKEVKQQCEEEKAANLRLQQILDGKELEIKKDISQLETQIHGLTVVENCCSNLNYLTKRMDILEKGLHSISESQKNLHSRVDNELSPDTLGIIFEGRFVDLEARLNFTEREIGSCCSNVEDTMRGLMGSELDGTKALFENKITTLEERLITIVGDVNNVSSAVGLDGAVMPFLEGELATITRETEEKMEVLQSQLTNLETTCLLGCTSKSKDVEIFRTEIEDCQNKNQDLLLRMDSNSDLLHKLNITILEMQRRIEEETAGTLQGEITLLKINLNTMTKSLTGLKDSVSQYSDSVLHVNSSLDDHERKIEDEVHSLQEMISNQDSQLFFSNKRVLNLKGDLERLRSRIINDLSNCKSLALGLQKEVLQFDDRVAQIEDTCGRLGSVTGSLEHIRNELENNAGSMWGYMNHINKTLATHSEEITVLKNNLLDCQAKITELSEDVGLLEGKLKNNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLR3</accession>
    <name>A0A6I9XLR3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Nicotinamide riboside kinase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NMRK2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>beta-D-ribosylnicotinate + ATP = nicotinate beta-D-ribonucleotide + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25568"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.173"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>beta-nicotinamide D-riboside + ATP = beta-nicotinamide D-ribonucleotide + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14017"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15927"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the uridine kinase family. NRK subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914896.1">
        <property type="nucleotide sequence ID" value="XM_014059421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLR3"/>
    <dbReference type="GeneID" id="106543412"/>
    <dbReference type="KEGG" id="tsr:106543412"/>
    <dbReference type="CTD" id="27231"/>
    <dbReference type="OrthoDB" id="10041966at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061769">
        <property type="term" value="F:nicotinate riboside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050262">
        <property type="term" value="F:ribosylnicotinamide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019363">
        <property type="term" value="P:pyridine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02024">
        <property type="entry name" value="NRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000853">
        <property type="entry name" value="Nicotinamide riboside kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013914896.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="23620" checksum="B2F5E4715851F120" modified="2020-10-07" version="1">MKVILGIGGITNGGKTTLTNKLTQSLPNCSVVHQDDFFKPQDEIPVEDGFKQYDVISALDMDAMMNTISAWRKNPIKFERSHGINTLDTSTIEEDDDTIHILIVEGFLLYTYRPLIDIFDARYFLSIPYEECKRRRSSRNYTVPDPPGLFDGHVWPMYIKHKKVMKNLNVDVVHLDGTKSREELFDTVYNHIQNNLEELIHMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLZ7</accession>
    <name>A0A6I9XLZ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Factor in the germline alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transcription factor FIGa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FIGLA</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Heterodimer with TCF3/isoform E12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013911858.1">
        <property type="nucleotide sequence ID" value="XM_014056383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLZ7"/>
    <dbReference type="GeneID" id="106541055"/>
    <dbReference type="KEGG" id="tsr:106541055"/>
    <dbReference type="CTD" id="344018"/>
    <dbReference type="OrthoDB" id="5976910at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11422">
        <property type="entry name" value="bHLH_TS_FIGLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000068">
        <property type="entry name" value="factor in the germline alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF57">
        <property type="entry name" value="FACTOR IN THE GERMLINE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="64"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013911858.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22402" checksum="BC0D630AA2DDB3A0" modified="2020-10-07" version="1">MAQEASGEDSQQLPRLFLAPPAEALGEILNKQFGPLPFMAPITRMKRKPSGYYYSTENREQVLGRRRAANAKERERIKNLNSGFSKLKSIVPLIPKDRKPSKVDMLKATADYIRLLRLILKETGGLQGEEVANGPLPGPVAEPSEKCLPGRIQSQRPSCYIKTENGLELVWTNQMMQAPVGTWGETVLPAYMGIVELHKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPZ3</accession>
    <name>A0A6I9XPZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acetyl-CoA C-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ACAT1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>propanoyl-CoA + acetyl-CoA = 2-methyl-3-oxobutanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:30719"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57335"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:30720"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:30721"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="5"/>
    <dbReference type="RefSeq" id="XP_013915871.1">
        <property type="nucleotide sequence ID" value="XM_014060396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPZ3"/>
    <dbReference type="GeneID" id="106544193"/>
    <dbReference type="KEGG" id="tsr:106544193"/>
    <dbReference type="CTD" id="38"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000007">
        <property type="entry name" value="acetyl-CoA acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF156">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026757819" description="acetyl-CoA C-acetyltransferase" evidence="17">
        <location>
            <begin position="21"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase N-terminal" evidence="18">
        <location>
            <begin position="26"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="19">
        <location>
            <begin position="291"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="14">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="242"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013915871.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="43513" checksum="CD11A4F04B1F9A97" modified="2020-10-07" version="1">MDKFCIILKTLSLTLNGCNAFPFQEVVIVSAVRTPIGSFQSSLFSLPATNLGSIAIKGAIEKAGIPSQEVKEVYMGNVIQAGEGQAPARQATLGAGLPVSTPCTTVNKVCASGMKSIMMAAQSLMCGSQDVMVAGGMESMSNVPYTMTRGTTPYGGVKLEDLIVKDGLTDVYNKIHMGNCAENTAKKFSISREDQDTYAIKSYTKSKEGWESGIFAKEIVPVTISQKGKPDTEVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTSEAAKRLNVKPLARIVGFADAAVDPIDFPIAPAHAVPKLLAQTGLKKEDVKMWEINEAFSVVVLANIKMLDIDPQKVNIHGGAVSLGHPIGMSGARIVVHMTHALKQGEHGLAGICNGGGGASAILIQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQ89</accession>
    <name>A0A6I9XQ89_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ral guanine nucleotide dissociation stimulator-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106544460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013916212.1">
        <property type="nucleotide sequence ID" value="XM_014060737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQ89"/>
    <dbReference type="GeneID" id="106544460"/>
    <dbReference type="KEGG" id="tsr:106544460"/>
    <dbReference type="OrthoDB" id="26687at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="1">
        <location>
            <begin position="24"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013916212.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="14473" checksum="4944B4447A090E7B" modified="2020-10-07" version="1">NQTIKPAWNPDVEKRKASLKSVLSFQLTSQDKTPAVIQRAMLKHNLESDPAEDYELVQVISEDKELVIPDTANVFYAMNSQVNFDFVLRKKNSLDGQVKLRNRSSLTLPRTNRRGCWNNRNSKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQV2</accession>
    <name>A0A6I9XQV2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Prostaglandin-H2 D-isomerase</fullName>
            <ecNumber evidence="27">5.3.99.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Glutathione-independent PGD synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Lipocalin-type prostaglandin-D synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Prostaglandin-D2 synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">LOC106541989</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>prostaglandin H2 = prostaglandin D2</text>
            <dbReference type="Rhea" id="RHEA:10600"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:57406"/>
            <dbReference type="EC" id="5.3.99.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Rough endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="EC" id="5.3.99.2" evidence="27"/>
    <dbReference type="RefSeq" id="XP_013913058.1">
        <property type="nucleotide sequence ID" value="XM_014057583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQV2"/>
    <dbReference type="GeneID" id="106541989"/>
    <dbReference type="KEGG" id="tsr:106541989"/>
    <dbReference type="OrthoDB" id="9048943at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004667">
        <property type="term" value="F:prostaglandin-D synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043303">
        <property type="term" value="P:mast cell degranulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002345">
        <property type="entry name" value="Lipocalin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430:SF86">
        <property type="entry name" value="PROSTAGLANDIN-H2 D-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01254">
        <property type="entry name" value="PGNDSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="22" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="23" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="24" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0467">Mast cell degranulation</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="10" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="32">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="34">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00030654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00031917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00032350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_013913058.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13851" checksum="FA886032EEEFEE4E" modified="2020-10-07" version="1">TVVSPTEDGNLDIASTYPKLDQCETKRTVFLRTEEPGRFTYTSPWSGSAHNIRVLETNYNEYALLGDTITKGADTFTMVTLYGRQKQLRPELLAKFTQTALSQGLAQEDVLVLPRTDLCMGETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTG2</accession>
    <name>A0A6I9XTG2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein LOC106546896</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106546896</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013919359.1">
        <property type="nucleotide sequence ID" value="XM_014063884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTG2"/>
    <dbReference type="GeneID" id="106546896"/>
    <dbReference type="KEGG" id="tsr:106546896"/>
    <dbReference type="OrthoDB" id="44867at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="4">
        <location>
            <begin position="462"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013919359.1"/>
        </source>
    </evidence>
    <sequence length="539" mass="60763" checksum="FEB875878FD9376B" modified="2020-10-07" version="1">MGQAEELIRTAKKLDKMVSRKNTVRRGENPNHLRTTCCFLRCAFAGSLLLTQTRLLQALRVCAIRRLFVCLFCSGFRVTLGRVCKWFSRRRRGVASWKTRGWREEGKRKWGYTFLSVKGPVRFASGKRASRNATHKEKETMYRQISSSVICQTHFSSQDGFLAQGLPGLHGRFLDGVGVHGAAAVDADALHLLVLFVQPGDVPRHPRRAVGFHFAPEGGVEVLVELGVQEVPRCGFLQILQEHQRFVEVLQEHHQMKTPVDLANVLLDQRAVGIQVVVIAQVEEKEILEVVRVVPFWVVVMPRVPQEFSQLVIAQHLPGQRDDPPKVVERRLKEVGRIPQHRHEDGVRRQQLAHLLVLEAHVVADDVEAHAGLVVFHLVAVEERRVVEIAPSVGEGELEAVLLPETEEDIEEGAAGCFIYSLCHADLQGWDRVGVQKQHEAGEKRPQDEAFTHGLPVLPLGQHYDRLKEEAGENAVWIPVMKILLSLFQTTKIGVAVNAVRKHCSDKEVVALAKLLIKNWKRLLVVLYHGIFKLAITYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XU83</accession>
    <name>A0A6I9XU83_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Max-interacting protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MXI1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013917632.1">
        <property type="nucleotide sequence ID" value="XM_014062157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XU83"/>
    <dbReference type="GeneID" id="106545555"/>
    <dbReference type="KEGG" id="tsr:106545555"/>
    <dbReference type="CTD" id="4601"/>
    <dbReference type="OrthoDB" id="5920083at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18930">
        <property type="entry name" value="bHLHzip_MXI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000014">
        <property type="entry name" value="Max dimerization protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969">
        <property type="entry name" value="MAX DIMERIZATION, MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969:SF13">
        <property type="entry name" value="MAX-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="121"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="90"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="163"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013917632.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="31763" checksum="104B22872DD54254" modified="2020-10-07" version="1">MGKRGRPRKELRCEGDAGKAVLGQQQQPVIDNTSRCPFSDIFNTSSEDSMEKINTFLQNVQILLEAASYLEQIEKENKKCEHGYASAFPSMTSLGLQDPKPLQRLSRSRKHSSGGNSTGTTNRSTHNELEKNRRAHLRLCLERLKVLIPLGPDCTRHTTLGLLNKAKAHIKKLEEADRRSQHQLENLEREQRFLKRRLEQLQSPQDIERIRMDSTGSTMSSDRSDSEREEIEVDVESTEFSHGDVDDISTTSTSDIDDHSSLQSIGSDEGYSSASVKLSFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUJ6</accession>
    <name>A0A6I9XUJ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger protein 202-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106545643</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013917752.1">
        <property type="nucleotide sequence ID" value="XM_014062277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUJ6"/>
    <dbReference type="GeneID" id="106545643"/>
    <dbReference type="KEGG" id="tsr:106545643"/>
    <dbReference type="OrthoDB" id="9036202at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000005">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SCAN box" evidence="5">
        <location>
            <begin position="81"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="6">
        <location>
            <begin position="316"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013917752.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="46300" checksum="931655CCFCDE13F2" modified="2020-10-07" version="1">MAHFGGSSYHSAPLTKSPFANPGQTLDGCDPASLYTPKSGSRDPEGNGSRVALLRSTEKFWRRTKEDNVLEGDTTRWAGEHQLCRAFFYEETEEPRELCTRLHRLSRQWLKPEKHTKAQMLDLVVLEQFLAVLPPKMESWVRECGAETSSQAVALAEGFLLSQAEEKKQGEMQVQDPFIEVGVQNLQAHRDMSSLSQELAFGGISQEDPAQVTSEMNGATLRFPAEMSLLCGGTETAVVIPVQVQDPFMEVDAQDPREHKDMSSLTQELAFGGISQEDPAQITSEGKGAILRFPAEASLFCGDSETAAVIPTQNHMLFEDIAIFFSEEEWALLNPDQKLLYREITLENARNVTSIGERWEDEDSKKEMIEHLPSVKNEIGERMFQCQWGVQSPEEKQLKVEPEIALAFHHVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVJ8</accession>
    <name>A0A6I9XVJ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Doublesex- and mab-3-related transcription factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DMRT3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914678.1">
        <property type="nucleotide sequence ID" value="XM_014059203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVJ8"/>
    <dbReference type="GeneID" id="106543242"/>
    <dbReference type="KEGG" id="tsr:106543242"/>
    <dbReference type="CTD" id="58524"/>
    <dbReference type="OrthoDB" id="5842031at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046661">
        <property type="term" value="P:male sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14419">
        <property type="entry name" value="CUE_DMA_DMRTA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005173">
        <property type="entry name" value="DMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF120">
        <property type="entry name" value="DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03474">
        <property type="entry name" value="DMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="10">
        <location>
            <begin position="29"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="8">
        <location>
            <begin position="29"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="142"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013914678.1"/>
        </source>
    </evidence>
    <sequence length="457" mass="49726" checksum="F17BECEF063F7AF1" modified="2020-10-07" version="1">MNGYGSPYLYMGGPVAQPARAPLQRTPKCARCRNHGVLSWLKGHKRYCRFKDCTCEKCILIIERQRVMAAQVALRRQQANESLESLLPDTLRALPAAAAASAAPPPASATPPGHPGAAGILFKKENIASISLLVFSNMTEERLGDVSGGDSTETYSDKDTDQRSSPEVTKTKNSFTPESPEIVSVDEGGYAVQKSSGSTDSRPESPKYQPEQNHFLIEGPSGTVSLPFGLKANRPPLEVLKKIFPNQKPTVLELILKGCGGDLVSAVEVLLSSRSSLGGGDRTSAEPEGLVLPSNGHLFEHTLSSYPISSSKWSVGSAFRVPDTLRFSADTSNVVPNPLAVPLQHPFPQPPRYPLMLRNTLARNQSSPFLPNDVTLWNTMTLQQQYQLRSQYVSPFSANSATVFRSSPVLPTRPSEDPRISLQEDGCPIVAKQPIYTEDDYDERSDSSDSRILNTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XVS4</accession>
    <name>A0A6I9XVS4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Neuroblastoma-amplified sequence-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106545994</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013918217.1">
        <property type="nucleotide sequence ID" value="XM_014062742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XVS4"/>
    <dbReference type="GeneID" id="106545994"/>
    <dbReference type="KEGG" id="tsr:106545994"/>
    <dbReference type="OrthoDB" id="19988at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029145">
        <property type="entry name" value="NBAS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15922:SF2">
        <property type="entry name" value="NBAS SUBUNIT OF NRZ TETHERING COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15922">
        <property type="entry name" value="NEUROBLASTOMA-AMPLIFIED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15492">
        <property type="entry name" value="Nbas_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neuroblastoma-amplified sequence N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013918217.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="23731" checksum="96D8C3ACD0F970C1" modified="2020-10-07" version="1">AVIHLSGRLTVWSIPSLKQLREWEQIDQPGYDEIKLEWKLSLEKRKKIKDKESFYPLIDINWWAENSVILARCSGALTVSSVKNLKNLLGKSCEWFESAPQVTSAHEGGFLCLECEVKFAPKRLRSEGRPGDEDEGEDDSDSDDEMSAKARYFGYLKQGLYFVTEMERFAPSRKRPRTIIKNYRLVNLRSTTPEELYQRKNYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWB0</accession>
    <name>A0A6I9XWB0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein C12orf40 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106543440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914933.1">
        <property type="nucleotide sequence ID" value="XM_014059458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWB0"/>
    <dbReference type="GeneID" id="106543440"/>
    <dbReference type="KEGG" id="tsr:106543440"/>
    <dbReference type="CTD" id="283461"/>
    <dbReference type="OrthoDB" id="6430388at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027883">
        <property type="entry name" value="Redic1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35158">
        <property type="entry name" value="CDNA SEQUENCE CN725425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35158:SF1">
        <property type="entry name" value="CDNA SEQUENCE CN725425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15089">
        <property type="entry name" value="Redic1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914933.1"/>
        </source>
    </evidence>
    <sequence length="599" mass="67605" checksum="D8BBF3D9C5B4DF10" modified="2020-10-07" version="1">MNWVGGSRRRIMLTKERRKQKDFFEKEKLKSKMKLVGVSPLKSSAVSLDLLNLYVVNQISTKKENAENIRKPIHVDINRGTQIPLRKQNVDFPKSPECRPAKLSLDDIQHRIQEEILDSRRKYLYEKASVHSEELGSSWPVNNKEEDSWFSTTAYRPEEFSAPDSEAQVGLYFQQLPSPGYGNSFDKDSGNSQANEEESLFGTINDQIKTNQDANFQPIASVFEEENQFLTFPPSQSYCSFANKSHIDQLFTDFGNAKEIFGKPSLYDSEETQQVTGPTHQTAERDLTSILTAPEMICTLNNQSLNAINQNPVWNQFKHCSVQKRNPVGFSDQQKLTRFFDNIGGFKKQKKCTKAPKPIKNYLKNSRKKDQSGPNMDYLKIFAHETLDKEFYSDSDQHWKQSKQNDDDFQLSSQSPTYSPEEADRYTSSSSDESKPEERDEGASSDSQGSFSRTSRDPCSGPEDVRQFHGRHWNQTNIQPQQKLATTPRKDTLRRAGLPMSGVNDKGKREASSQTEACADPVEKSNAAVQCDIIQSCPCKKELSVAHSAEMVTSTSKAEATGGQNVTADSAVLRPPNSNSKLCLPKQMVLKWQGQPSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWT7</accession>
    <name>A0A6I9XWT7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106546024</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013918256.1">
        <property type="nucleotide sequence ID" value="XM_014062781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWT7"/>
    <dbReference type="GeneID" id="106546024"/>
    <dbReference type="KEGG" id="tsr:106546024"/>
    <dbReference type="OrthoDB" id="9829410at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0552">Olfaction</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013918256.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="34926" checksum="A8CD0153664A44A9" modified="2020-10-07" version="1">MEKEKHTTVMNFILLGFTDNQNLQLILFVTFLLIYIASLTGNITLMTLIYGSPNLHTPMYFFIGNLSFLDLWYSSVYSPKILVNSICEDKSISFGGCAAQFFFSAGLAYSECYLLAAMAYDRYMAISNPLLYATHMSRKLCTGLVTLSYVSGFINATIITIKTFTLSFCNGNVIDDFFCDLPPLVKLSCNVPESYQSVLFFILVSNVIIPLALILASYVLILAAILKIRSTEGRLKAFSTCASHLTAVTLYYGSILFIYSRPSSSYALKRDKVVSVFYTVVIPMINPLIYSLRNEEVKKALKKKAMKWKMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXB6</accession>
    <name>A0A6I9XXB6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein C10orf105 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC106539000</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013909139.1">
        <property type="nucleotide sequence ID" value="XM_014053664.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013909140.1">
        <property type="nucleotide sequence ID" value="XM_014053665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXB6"/>
    <dbReference type="GeneID" id="106539000"/>
    <dbReference type="KEGG" id="tsr:106539000"/>
    <dbReference type="OrthoDB" id="8955919at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039954">
        <property type="entry name" value="DUF5527"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38325:SF1">
        <property type="entry name" value="GENE, 17455-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38325">
        <property type="entry name" value="MCG55969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17665">
        <property type="entry name" value="DUF5527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013909139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013909140.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15545" checksum="AE1AC13E6F9E1C3E" modified="2020-10-07" version="1">MSAKESVNETLPSTGVPELLNATTELALLSSEPTEQNLDSLPIIIALICIFLLLTIGLLFVTLCKPAALDPSVHRPHECMPYHFEDATEPQLKLWKRLGSLRQSICSVKRGRPVSHRHETWSRKGPINQDWSIMESTAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXN1</accession>
    <name>A0A6I9XXN1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">LIM and cysteine-rich domains protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LMCD1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013909240.1">
        <property type="nucleotide sequence ID" value="XM_014053765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXN1"/>
    <dbReference type="GeneID" id="106539083"/>
    <dbReference type="KEGG" id="tsr:106539083"/>
    <dbReference type="CTD" id="29995"/>
    <dbReference type="OrthoDB" id="10069167at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09340">
        <property type="entry name" value="LIM1_Testin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09829">
        <property type="entry name" value="PET_testin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000005">
        <property type="entry name" value="Testin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010442">
        <property type="entry name" value="PET_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033724">
        <property type="entry name" value="PET_testin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047120">
        <property type="entry name" value="Pk/Esn/Tes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211:SF0">
        <property type="entry name" value="LIM AND CYSTEINE-RICH DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06297">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51303">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PET" evidence="10">
        <location>
            <begin position="94"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="236"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="301"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="212"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013909240.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="40339" checksum="080F1DD0FD8F500D" modified="2020-10-07" version="1">MDLSSGIQKVSITHQPSGRGAPCLKCREKCAGFEPHSWRKICKSCKCSQEDHSLSSDAEDDRKIGRLLADSKYSSLTARVKGGDGIRIYKRNRMIITNPIVSRKDPTFDTVTYEWAPPGLTQKLATQYMSLISKEMQPVAGTEGAYYRRRRLMRQLPLYDQDPSQCRGLSENEIKLMEEFIKQYKDNVLGVGEVALLGQGGVTKEEGKSSDKNTPASKEAASTNGTLGHVSKGQDLCCDFCKQVLPAEFPVVYADRAGYNHQWHPTCFMCSKCSEPLVDLIYFWNNGAIWCGRHYCDSIRPRCAACDEIIFSDDYLQTEGLTWHKQHFTCLECESSLAGKPFILDASSLWCTSCSKSKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XXW6</accession>
    <name>A0A6I9XXW6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">RNA-binding protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RBM5</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RBM5/RBM10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909315.1">
        <property type="nucleotide sequence ID" value="XM_014053840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XXW6"/>
    <dbReference type="GeneID" id="106539141"/>
    <dbReference type="KEGG" id="tsr:106539141"/>
    <dbReference type="CTD" id="10181"/>
    <dbReference type="OrthoDB" id="29221at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12752">
        <property type="entry name" value="RRM1_RBM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000110">
        <property type="entry name" value="RNA-binding protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000114">
        <property type="entry name" value="RNA-binding protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041591">
        <property type="entry name" value="OCRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034991">
        <property type="entry name" value="RBM5_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948:SF21">
        <property type="entry name" value="RNA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17780">
        <property type="entry name" value="OCRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="115"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="15">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="248"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="660"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="14">
        <location>
            <begin position="756"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="433"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="523"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013909315.1"/>
        </source>
    </evidence>
    <sequence length="828" mass="94296" checksum="8C0079A72BFE6EF6" modified="2020-10-07" version="1">MGSDKRVSRTERSGRYGSIIDREDRDERDSRSRRRDSDYKRSSEERRNDRYDDYRDYDSRDYDGRDYDGRDYDSPERERERERRNSDKSEDGYHSDGDYGEHDYRNDINDEKESKTIMLRGLPITVTDYDIREIIESCEGPRPADVRLMKRKTGVSRGFAFVEFYHFQDATSWMEANQKKLLVQGKQIAMHYSNPRPKFEDWLCNKCCLYNFRRRLKCFRCGADKFDSEQEVPPGGPEAVQSVDYYCDTLILRNIAPHTVVESIMTALSPYASLAVNNIRLIKDKQTQQNRGFAFVQLSSAMDASQLLQILQSLQPPLKIDGKTIGVDFAKSARKDLLLPDGNRVSAFSVASTAIAAAQWSSTQPQSGEGGSVDFNYLQLGQDGYNQYTQDYQQFYQNQAGTLDTDRTTTSGGPGTAGTTTAAVVSQSPQLYNQQTPSADTPTQAAQPSTSSQVSSSSPTSIVPGTKYAVPDTSTYQYDESSGFYYDPVTGLYYDPNSQYYYNAVTQQYLYWDGEKETYMPAAEGTSYQQNNGPSAKEGKEKKEKPKSKTAQQIAKDMERWAKSLNKQKENFKNSFQPLNAREEERRESAAADAGFALFEKKGTLSERQQIITEVIKNGDDDNPLKRGLVAAYSGDSDNEEDFLERLENEEEKLTDWKKLACLLCRRQFPNKEALVRHQQLSDLHKQNMDIYRRSRLSEQELEALEMREREMKYRDRAAERREKYGIPEPPEPKRKKAFDAGTVNYEQPTKDGIDHSNIGNKMLQAMGWREGSGLGRKCQGITAPIEAQVRMRGAGLGAKGSSYGVSTADSYKDAVRKAMFARFTEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y031</accession>
    <name>A0A6I9Y031_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vesicle transport through interaction with t-SNAREs homolog 1B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">VTI1B</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VTI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916258.1">
        <property type="nucleotide sequence ID" value="XM_014060783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y031"/>
    <dbReference type="GeneID" id="106544499"/>
    <dbReference type="KEGG" id="tsr:106544499"/>
    <dbReference type="CTD" id="10490"/>
    <dbReference type="OrthoDB" id="430637at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903076">
        <property type="term" value="P:regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15890">
        <property type="entry name" value="SNARE_Vti1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000002">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.400">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038407">
        <property type="entry name" value="v-SNARE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007705">
        <property type="entry name" value="Vesicle_trsprt_v-SNARE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF89">
        <property type="entry name" value="VESICLE TRANSPORT THROUGH INTERACTION WITH T-SNARES HOMOLOG 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05008">
        <property type="entry name" value="V-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="132"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916258.1"/>
        </source>
    </evidence>
    <sequence length="233" mass="26822" checksum="6BFFCDD754BB2014" modified="2020-10-07" version="1">MADRSIGSSEHLERLHEIFRGLHGELQSAPERLRGNLAVEEKKKLIREFDEKLKEANETLKEMEEELKYAPVSFRNQMMIKIRTYKGDLSTFHRKMKSTDLGVAPSARGNSKFGIFSKENEQRTQMQSQRVLLLQGTESLNRATQSLDRTHQIAAETDQIGSDIIEELGGQREQLERTKGRLVNTNENLSRSRKILRSMSRRLMTNKLLLSVIIILEVIILGGLVYYKFFTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2A9</accession>
    <name>A0A6I9Y2A9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U4/U6 small nuclear ribonucleoprotein Prp3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pre-mRNA-splicing factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PRPF3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013910935.1">
        <property type="nucleotide sequence ID" value="XM_014055460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2A9"/>
    <dbReference type="GeneID" id="106540374"/>
    <dbReference type="KEGG" id="tsr:106540374"/>
    <dbReference type="CTD" id="9129"/>
    <dbReference type="OrthoDB" id="10264544at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24162">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1390.10:FF:000003">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein Prp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013881">
        <property type="entry name" value="Pre-mRNA_splic_Prp3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027104">
        <property type="entry name" value="Prp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212">
        <property type="entry name" value="U4/U6-ASSOCIATED RNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08572">
        <property type="entry name" value="PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PWI" evidence="11">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="73"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="625"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013910935.1"/>
        </source>
    </evidence>
    <sequence length="700" mass="79262" checksum="8B4EE4AB07AD0DA1" modified="2020-10-07" version="1">MALSKRELDELKPWVEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAVEEGRSSRHSKSNSDRNRKRELKDVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPTESPGMLTKLQIKQMMEAATRQIEERKKQLSFISSPAPQPKAPSTTPPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNTNMVGLANLHAMGITPPAEIRHMGSNVQSPYTRKVELKDQSKPTPLILDEQGRTVDATGKEVELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTYFDARVSAAPAQRPKRTFRFHDKGKFEKIAQRLRTKAQLEKLQAEISQAAKKTGIHTSTKLALITPKKEVKEGDIPEVEWWDSYIIPNGMDIKATDLLKREDYFGITNLVEHPAQLSPPVDSDLPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLMPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTVEQRKAKKVKKLKEDISQGVHIAVYRVRNLGNPAKKFKIEANASQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDDESDEESMKKTNKCSLVWEGTAKERSFGEMKFKQCPTENMAREHFKKHGTEHYWDLALSESVLEPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2N6</accession>
    <name>A0A6I9Y2N6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Semaphorin-4A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106540498</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911095.1">
        <property type="nucleotide sequence ID" value="XM_014055620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2N6"/>
    <dbReference type="GeneID" id="106540498"/>
    <dbReference type="KEGG" id="tsr:106540498"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF15">
        <property type="entry name" value="SEMAPHORIN-4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026777250" evidence="3">
        <location>
            <begin position="23"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="4">
        <location>
            <begin position="27"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013911095.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="25566" checksum="CC026C876BB74DAC" modified="2020-10-07" version="1">MKPGRLPWLLGCFLIAAAAVGSKVVPRMTFQRGDPGREIGAFSQEGILNYDTFLLSADGETLYVGGRDAILALNITSSPISLKGKIPWSPSPSKRRECVFKKKSNETECFNFIRILVQLNETHLYTCGTCAFSPTCAFIDVENFTLVTDAKGEPLLQEGKGLCPFDPRHQNTALIVGGELYTGTMNNFQGNEPVISRTLGSRTILKTDSFLSWLHDDAAFVASFNVPGPPGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3P3</accession>
    <name>A0A6I9Y3P3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">25-hydroxycholesterol 7-alpha-hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106540924</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; bile acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911705.1">
        <property type="nucleotide sequence ID" value="XM_014056230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3P3"/>
    <dbReference type="GeneID" id="106540924"/>
    <dbReference type="KEGG" id="tsr:106540924"/>
    <dbReference type="OrthoDB" id="6692864at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008396">
        <property type="term" value="F:oxysterol 7-alpha-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024204">
        <property type="entry name" value="Cyt_P450_CYP7A1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF0">
        <property type="entry name" value="CYTOCHROME P450 7B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000047">
        <property type="entry name" value="Cytochrome_CYPVIIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="9 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013911705.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="58175" checksum="BCCBAE742E5CD446" modified="2020-10-07" version="1">MGLLEWLPSGVYGLALATALAFGAFCAFGRRKRHPGEPPLINGWIPFLGEALNYRKNATEFLLTLSKKHGDIFTVHMAGKYITFIINPLQYPIVTKSSKYLEFSEFADQISSQAFDHPPTINEKYPDLNEHIHRNYQHLVGKSLDTLSDNMMRNLQIVFKTKFSQLVDWEMVKMHKFCFSIIFEASFKTFFGRDPYNEDYNVTDEIGEKFLKFDASFSYLALNIPITLLGPTKKIRKELINFFNPKKMEKWLDVSKVIQNRIDMLENYNLLSDYDKAAHHFAFLWASMGNTIPATFWAIYYLLRHPEAFAVVRDEIDHLLQSTGQERKPGCSIYLSREQLDNLVYLESAINETLRLCSSSMNIRKIKENFILQFEENRGVSLRKGDLFAIFPPILHMDPEIYEEPQTYKFDRYVENGKKKTTFLKQGKKLKYFLMPFGSGSSMCPGRFFAMNEIKLFVVLALVYFDMNIMEDKQLGQGKDRIGLGILLPDSDIIFRYKLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4T4</accession>
    <name>A0A6I9Y4T4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">STK39</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013919213.1">
        <property type="nucleotide sequence ID" value="XM_014063738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4T4"/>
    <dbReference type="GeneID" id="106546782"/>
    <dbReference type="KEGG" id="tsr:106546782"/>
    <dbReference type="CTD" id="27347"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010820">
        <property type="term" value="P:positive regulation of T cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000043">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000114">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000068">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF14">
        <property type="entry name" value="STE20_SPS1-RELATED PROLINE-ALANINE-RICH PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="42"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="341"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="359"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013919213.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="57815" checksum="3F9435E07C55AACB" modified="2020-10-07" version="1">MAEQSDSPVHVQQPQPAPAAPPPPAPTPAPQAAGWPICRDAYELQEVIGSGATAVVQAALCKPREERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLGGGSMLDIIKYVVSRGEHKSGVMEESIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGSAPYHKYPPMKVLMLTLQNDPPTLETGIEDKEMTKRYGKSFRKLISLCLQKDPSKRPTAAELLKCKFFQKAKTREYLIEKLLTKTPDIGQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEEDAEISVYSSNIPDQIQNLSIKGSQAHSAITNEYSSLPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPRALKTLTFKLASGCDGTEIPDEVKLIGFAQLSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4V9</accession>
    <name>A0A6I9Y4V9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CASP8 and FADD-like apoptosis regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CFLAR</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013912610.1">
        <property type="nucleotide sequence ID" value="XM_014057135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4V9"/>
    <dbReference type="GeneID" id="106541631"/>
    <dbReference type="KEGG" id="tsr:106541631"/>
    <dbReference type="CTD" id="8837"/>
    <dbReference type="OrthoDB" id="8816507at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000016">
        <property type="entry name" value="CASP8 and FADD-like apoptosis regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169:SF3">
        <property type="entry name" value="CASP8 AND FADD LIKE APOPTOSIS REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169">
        <property type="entry name" value="DED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00031">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DED" evidence="4">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="DED" evidence="4">
        <location>
            <begin position="97"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013912610.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="26535" checksum="F67A414C903BAB7D" modified="2020-10-07" version="1">MTVFSVPASLLYKLEQELDTDEKETVVFLCSDLVPDESISDVLQLLTALNEKEILTTINLSELLYRLKRFDLLKKFLGTGRAAVEVNLAHHSQMLSKYRVLMTEINEDLDKEDLRSLSFLLKNHLGKSHKEKSFLAIITDLEKLELISPMHLDLIENAFLTIHRRDLAKKIQKYKLEARFPNMNAKTLQVSLPKLSLADPPEPVNKGRVMNGASAAQGKPCYIFIAILSLTTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6P9</accession>
    <name>A0A6I9Y6P9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Large neutral amino acids transporter small subunit 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106542871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914190.1">
        <property type="nucleotide sequence ID" value="XM_014058715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6P9"/>
    <dbReference type="GeneID" id="106542871"/>
    <dbReference type="KEGG" id="tsr:106542871"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF113">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914190.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="15427" checksum="174FE962DB7FA768" modified="2020-10-07" version="1">MLVTSDIYTLINYVGFINYLFYGVTVAGQVVLRWREPHRPRPIKVSLFFPIIYLLFWAFLLVFSLYSEPLVCGIGLAIMLTGVPVYFLGVHWKNKPPSFNSMVGECCRRPPPQGRESWDRPKMGAFCSRREYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y774</accession>
    <name>A0A6I9Y774_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KN motif and ankyrin repeat domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">KANK1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914680.1">
        <property type="nucleotide sequence ID" value="XM_014059205.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543244"/>
    <dbReference type="KEGG" id="tsr:106543244"/>
    <dbReference type="CTD" id="23189"/>
    <dbReference type="OrthoDB" id="5406014at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000017">
        <property type="entry name" value="KN motif and ankyrin repeat domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047184">
        <property type="entry name" value="KANK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021939">
        <property type="entry name" value="KN_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168:SF19">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12075">
        <property type="entry name" value="KN_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026930872" evidence="8">
        <location>
            <begin position="18"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="1142"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="1214"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="1247"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="786"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="970"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1311"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="450"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="970"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="997"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013914680.1"/>
        </source>
    </evidence>
    <sequence length="1334" mass="146378" checksum="14D917CDBD8C4631" modified="2020-10-07" version="1">MLLFYVLNICVVALKFSENEENAINGEDDKIQKDPYFVETPYGYQLDLDFLKYVEDIQKGNTIKKLNIQKKRKVTPPFAPVKIPSNQYSGWTSNESLSSSNSDENKHSSLLFTAQSQLAGTTSAKPSLAFEASPSFLTIPESKPLLPPSPQLPRHNLRVTKTLMETRRRLEQEKMAMQPIPGETRRPRLSSFGAMGSTGSLPSFVGSSSQNQMCQQFQNGYQGNGECSLYLASSLGSSIRHSPLSSGVSTPVTNVSPVHLQHIREQMAIALKHLKELEEQVKTIPVLQVKISVLQEEKRQLVAKLKSQRMVNQNDTGTFRKRSYSAGNAEQWKNLSSVRGGGELYIDCEEDTGNVGHSTEQVEEFRHLTAEMQALEKKIQDSSYDISSVRGNRANVAKENRSIAVGADVSMNDIVVYNRALRACRDVAVGTEVKTKDFVAGVSEEMLGLSTEAEREIELQHQMIESLKEKIYRLEVQLREATYDREMIKLKHELQAAGSRKKSDKALMAQPLVFSKAVGTEIQMRDQMVGNHVDIVDSCVGNSPQMSSIGVLCRPVLQSSAVGPDVPMNWWIVKERNEMCDRCIGSYVETHNKCVGTEMSFCETGVNTEELVDSLSLHKRELQIKEAWSLGYRDSLDLTLCPAKETTSCSTNTETPCRANSGIMAVPCTINQGTNTVLEKADGFTNTEMTILTDSSTNTLLSTSDKQTNTVAVELKTVAVGDGRVKDVNTAAKTRSIGIGTLLSTTTSFDKSSLTKTKDCGVGQISIHENYLVGLKMRNIACGPPPVSLAPPSTRSIGVGDDSVDDSGNPDTDSPFPLPEIRTGLDHYIERVQKLVQEQQMLLAENYAELADAFGEPHSQIGSLNSQLISTLSSINTVMKYASTEELRSLEVPKQCTDKPTATGSSKLMLGQEIGTINEEKTAIIGMRQDQKILTSQDSTLSSINLTDDQLASGLYVCANDGMLKSIMKKKDGKKDENTKKNLQFVGINGGYETTSSDDSSSEESSSSESDDECAGNDYVQIENSSVLEREHRLNRKDVKPVQEEEIMHVQEHEPENVEIRERYELSEKMLSACHFLKNSIDDPKAMSSKEIRFCLNTIQHEWFRISSQKSAIAAMVGDYIATFEEASPAVLRQIINMADGNGNTALHYSVSHSNFEIVKLLLDANVCNVNHQNKAGYTPIMLAALAAVEAEKDMKIVEDLFACGDVNAKASQAGQTALMLAVSHGRIDMVKALLACGADVNIQDDEGSTALMCASEHGHVEIVKLLLAQPGCNGTLEDNDGSTALSIALDAGHKDIAVLLYAHVNFSKTQSPGTPRLGRKPSPSPTHRSSFDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y806</accession>
    <name>A0A6I9Y806_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-amino-acid oxidase</fullName>
            <ecNumber evidence="4">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106544367</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013916090.1">
        <property type="nucleotide sequence ID" value="XM_014060615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y806"/>
    <dbReference type="GeneID" id="106544367"/>
    <dbReference type="KEGG" id="tsr:106544367"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF355">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Amine oxidase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013916090.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="32141" checksum="8AA2D62E6F966C78" modified="2020-10-07" version="1">MGDHEFKSHLNHKIQLGDLGFDEIVGGFDQLPMCMYQAIANNVYFNAQVTKIQQNAEKVRVEYQTPAKTLSDVTADYVIVCSTSRATRRISFEPPLPPKKAHALRSIHYRSGTKIFLTCTKRFWEADGIHGGKSTTDLPSRFIYYPNHNFTSGVGVILAYVIADDANFFQALDIKSMGDIVINDLSFIHQLPKEEIQALCYPSVFKKWSLDKYAMGSITTFTPYQLQDFSESLAAPVGRIYFAGEHVAKVHGWVDNTIKTGLTAARDVNRASEKPSRIHLISDNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9P9</accession>
    <name>A0A6I9Y9P9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipase maturation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106546660</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the maturation of specific proteins in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipase maturation factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919055.1">
        <property type="nucleotide sequence ID" value="XM_014063580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9P9"/>
    <dbReference type="GeneID" id="106546660"/>
    <dbReference type="KEGG" id="tsr:106546660"/>
    <dbReference type="OrthoDB" id="5988002at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009613">
        <property type="entry name" value="LMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057434">
        <property type="entry name" value="LMF1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463">
        <property type="entry name" value="LIPASE MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463:SF5">
        <property type="entry name" value="LIPASE MATURATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06762">
        <property type="entry name" value="LMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase maturation factor 1/2 N-terminal" evidence="3">
        <location>
            <begin position="343"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013919055.1"/>
        </source>
    </evidence>
    <sequence length="561" mass="62697" checksum="CC05C1CF10623132" modified="2020-10-07" version="1">MAAFQHHLWPPTHARHSPEESIGIGQLVLIHSPKGLMDTCPQLPVGLILNVREGDQADREHPQGSCGENGPLHHSPELPEKTPDAGTPVSSLKDLPQQGEEPQGHQREGHRLQPLRELVVGEGCLGFKDSLLPVDGQPEVTAGPEGQEAVNSQLCQQGGQSPGPAATGFLPPLAQPQPHVLIVQQGEAQDDRQQVEEIVARRASRARRREGGGEEEAAMGSEAPRLARQLFLRGLAAAFLAAFASLYVQLPGLYGRDGILPARKMLRFTGKDFWQQLRDSPTLLWLGPHLGLDTEQGLELLCLMGTLLSFGALVLEPLRDSLVFLALWGLYLSVYQVGQVFLYFQWDSLLLETGFLAVLVAPMHLLKWRSTTWRAHDGVTFWLVRWLLFRLMFASGVVKLTSRCPTWWGLTALTYHYETQCIPTPSAWFAHQMPVWFQKLSVVGTYVIEIAIPPLFFVPLRRLRHFAFYSQVVLQALIILTGNYNFFNLLTVVLSFSLLDDEHVGLWLGQGRKKASGGWAWRLPPLLAELAVYGLLTFWTSRYFGLTINWEKRILESKTDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA11</accession>
    <name>A0A6I9YA11_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ras-related protein Rab-1A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RAB1A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919615.1">
        <property type="nucleotide sequence ID" value="XM_014064140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA11"/>
    <dbReference type="GeneID" id="106547090"/>
    <dbReference type="KEGG" id="tsr:106547090"/>
    <dbReference type="CTD" id="5861"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000069">
        <property type="entry name" value="Ras GTP-binding protein YPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013919615.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="22724" checksum="B2A8F4E3B33AF7D6" modified="2020-10-07" version="1">MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGSEKSNVKIQSTPVKQSSGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YA96</accession>
    <name>A0A6I9YA96_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17 18">Muscleblind-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17 18" type="primary">MBNL2</name>
    </gene>
    <organism evidence="15 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17 18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the muscleblind family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920038.1">
        <property type="nucleotide sequence ID" value="XM_014064563.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920039.1">
        <property type="nucleotide sequence ID" value="XM_014064564.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013920040.1">
        <property type="nucleotide sequence ID" value="XM_014064565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YA96"/>
    <dbReference type="GeneID" id="106547411"/>
    <dbReference type="KEGG" id="tsr:106547411"/>
    <dbReference type="CTD" id="10150"/>
    <dbReference type="OrthoDB" id="6285980at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000004">
        <property type="entry name" value="Muscleblind like splicing regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000002">
        <property type="entry name" value="Muscleblind-like 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054429">
        <property type="entry name" value="Znf-CCCH_Muscleblind-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF4">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22628">
        <property type="entry name" value="zf-CCCH_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013920038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013920039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013920040.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="42219" checksum="2E168AD6FD5716E2" modified="2020-10-07" version="1">MASIVAPVRDTKWLTLEVCRQFQRGTCSRSDEECKFAHPSKSCQVESGRVIACFDSLKGRCTRENCKYLHPPTHLKTQLEINGRNNLIQQKTAAAMLAQQMQFIFPGAPLQPMPSFSVGPTIGSNPALNFAPYLTPVTSGVGLVPTEIVPTQSVIVPGNPVSVSGSTTTQKLLRTDKLEVCREFQRGNCARGETDCRFAHPADSTMIDTNDNTVTVCMDYIKGRCLREKCKYFHPPAHLQAKIKAAQHQTNQAAAVAQAAATVMTQSTAKAMKRPLEATVDLGFPPAVLHPLPKRQALEKNNGASAVFNPSFLHYQQALASAQLQQHASFIPTGSVLCMTPATSIDNPEIVNRNGMECQEALLRTAKHCYCTYNTVSSLTELPQTAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAC3</accession>
    <name>A0A6I9YAC3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CFAP20</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920175.1">
        <property type="nucleotide sequence ID" value="XM_014064700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAC3"/>
    <dbReference type="GeneID" id="106547495"/>
    <dbReference type="KEGG" id="tsr:106547495"/>
    <dbReference type="CTD" id="29105"/>
    <dbReference type="OrthoDB" id="7486196at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFA20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013920175.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="22762" checksum="99A2DB1224E45077" modified="2020-10-07" version="1">MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRIYFSDRLYSEDELPAEFKLYIPVQNKAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB79</accession>
    <name>A0A6I9YB79_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMME syndrome candidate gene 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">AMMECR1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921371.1">
        <property type="nucleotide sequence ID" value="XM_014065896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB79"/>
    <dbReference type="GeneID" id="106548515"/>
    <dbReference type="KEGG" id="tsr:106548515"/>
    <dbReference type="CTD" id="9949"/>
    <dbReference type="OrthoDB" id="24630at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.150:FF:000001">
        <property type="entry name" value="AMME syndrome candidate gene 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.150">
        <property type="entry name" value="Hypothetical protein ph0010, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF2">
        <property type="entry name" value="NUCLEAR PROTEIN AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013921371.1"/>
        </source>
    </evidence>
    <sequence length="152" mass="17778" checksum="D3883ED395BA7FE6" modified="2020-10-07" version="1">MNLHSGLREYTLTSALKDSRFPPMTRDELPRLFCSVSLLTNFEDVCDYLDWEVGVHGIRIEFINEKGSKRTATYLPEVAKEQGWDHIQTIDSLLRKGGYKASITNDFRKTIKLTRYRSEKMTVSYTEYLAHRQHHHFQNGIGHTLPPYNHYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC56</accession>
    <name>A0A6I9YC56_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Forkhead box protein F2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FOXF2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921702.1">
        <property type="nucleotide sequence ID" value="XM_014066227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC56"/>
    <dbReference type="GeneID" id="106548777"/>
    <dbReference type="KEGG" id="tsr:106548777"/>
    <dbReference type="CTD" id="2295"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="term" value="P:animal organ morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Forkhead box F1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
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    <dbReference type="InterPro" id="IPR051770">
        <property type="entry name" value="Forkhead_box_regulator"/>
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        <property type="entry name" value="TF_fork_head_CS_1"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="FORKHEAD BOX PROTEIN BINIOU"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORKHEAD BOX PROTEIN F2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORKHEAD"/>
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    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="48"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="48"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="24"/>
            <end position="34"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013921702.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="39527" checksum="D533305E59D578AF" modified="2020-10-07" version="1">TSSTGGSSTSTTPGGAGGGGGTNSAGCASASAPSKTKKASSGLRRPEKPPYSYIALIVMAIQSSPSKRLTLSEIYQFLQARFPFFRGAYQGWKNSVRHNLSLNECFIKLPKGLGRPGKGHYWTIDPASEFMLEEGSFRRRPRGFRRKCQALKPTMYHRMVNGLSFLPQGFDFQAPPAAPLGCHPSGYNPLDVMQAGAAYDAHHVPHMSPNPGSTYMASCPVPSNGPDYGPDSSSSPVPSSPAMASAIECHSPYGSPAGHWTASAASPYLKQQALQPPPPPTAAAPAGIHSGMASYSLEQSYLHQNGREDLSVGLPRYQHHPSPVCDRKDFVLNFNGISSFHPSASSSYYHHHHHHHQTVCQDIKPCVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD23</accession>
    <name>A0A6I9YD23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ATP7B</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013922053.1">
        <property type="nucleotide sequence ID" value="XM_014066578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD23"/>
    <dbReference type="GeneID" id="106549049"/>
    <dbReference type="KEGG" id="tsr:106549049"/>
    <dbReference type="CTD" id="540"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060003">
        <property type="term" value="P:copper ion export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000015">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000092">
        <property type="entry name" value="copper-transporting ATPase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000144">
        <property type="entry name" value="copper-transporting ATPase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF29">
        <property type="entry name" value="COPPER-TRANSPORTING ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00943">
        <property type="entry name" value="CUATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0187">Copper transport</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="718"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="746"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="16">
        <location>
            <begin position="199"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013922053.1"/>
        </source>
    </evidence>
    <sequence length="852" mass="92273" checksum="97DE4D2960E4DD0C" modified="2020-10-07" version="1">MIKIVEGIGFQASLAKKDPKDHNLDYKEEIQQWRKSFLCSLVFGIPVLVLMVYMLLPIDNQHGSMVLEQNLIPGLSILNLLFFVLCTLVQFLGGWYFYVQAYKSLKHKMANMDVLIVLATSTAYAYSCIILIVAITEKAEQSPITFFDTPPMLFVFIALGRWLEHIAKSKTSEALAKLISLQATEAVVVTLGPDNCIIKEEQIPVELVQRGDIVKVVPGGKFPVDGKVIEGSSMADESLITGEAMPVTKKPGSVVIAGSLNAHGSVLVNATHVGSDTTLAQIVKLVEEAQMSKAPIQQLADRFSGYFVPFIIIISTATLITWIIIGFIQFDVVLKYFPHHSKQNSQAEIILRFAFQTSITVLCIACPCSLGLATPTAVMVGTGVAAQNGILIKGGKPLEMAHKVKTVMFDKTGTLTYGVPRVTRVLLLGVTEATLPLKKFLALVGTAEASSEHPLGMAVTRYCKEELGTEILGCCKDFQTVPGCGISCNVSSPETLLGDQIEQYMTSLSPHIDGFDKGVKDQTPLPKARDTAVSHTYSVLIGNREWMRRNGLHIPSDVHEAMRDHEMKGQTAILVAINGALCGMMAIADTVKPEAALAMHILQNMGVDVVLITGDNRKTAKAIATQVGIRKVFAEVLPSHKVAKVQELQTSGRKVAMVGDGVNDSPALAQADVGIAIGTGTDVAIEAADVVLIHSNLLDVVASIQLSRKTMKRIRVNLFLALIYNLIGIPIAAGVFMPLGLVLHPWMGSAAMAASSISVLLSSLQLKCYKKTVLEKYEAEAQGCMKPLTPSQVSVHIGMDDWRRKPPKLATWDSISQVSLCSLTSDVARGALEDGADKWSLLTNNQDEDQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YEK1</accession>
    <name>A0A6I9YEK1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein dopey-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DOPEY2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922608.1">
        <property type="nucleotide sequence ID" value="XM_014067133.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106549471"/>
    <dbReference type="KEGG" id="tsr:106549471"/>
    <dbReference type="CTD" id="9980"/>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056459">
        <property type="entry name" value="TPR_DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF23">
        <property type="entry name" value="PROTEIN DOPEY-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24601">
        <property type="entry name" value="TPR_DOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="8">
        <location>
            <begin position="28"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="9">
        <location>
            <begin position="343"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like TPR" evidence="10">
        <location>
            <begin position="1233"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="563"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="722"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1117"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1126"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1156"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013922608.1"/>
        </source>
    </evidence>
    <sequence length="1580" mass="179149" checksum="9A593E031E8A1C32" modified="2020-10-07" version="1">MGEEGVPQSILIWSFKMDMEEQELLGDYRYRNYSSAIDKALRNFESSSEWADLISSLGKLNKALQSNLKYSLLPRRLIISKRLSQCLHPALPTGVHIKALESYEVIFKIIGTKCLAKDLFLYSSGLFPLLGVAALSVKPVLLDLYENYFLPLQKSLLPSLQAFLIGLLPGLEEGSEFYDRTDALLVKLSLVIGKEVFYSALWASVLVSPSVRLPASLFVVNHINRAIPGKQQNYIMGTDPNLTIQAICVSMLDSNVLVQRNTLEVVLFFFPLYSSLDPNESAIPLHRSDLVHLLSAAAQTLLRRDMSLNRRLYSWLLGSDIKGKASVPALSINASLEDYAHSFFEKYSKELLVEGLIEILHQKFSESDLEEQHHAYLKPFRILISLLDKPEIGPLVIADLFLEVIRAFYRYCKETLGSDLKLSYSQTGNVLTSAIKENKNASEIVKTANMLITSLNTDFLWDYMTGCIEDCLSDKNKSTQTQLLTNGATISELCTLTVFLLDVIPLELYSEVQSLYLPQMLNCMVQSLLENMEVLSLSEFTYMLRTCFKVLSKVQMPSAYLSTETGSSDSSPVKEDNQEISSESKVLEDEEDIPLPSIKSEDSGIALSASSPELLQHLRMPRIIPDKDDVWKKGGSLQMTLHYIQELVAKFASKHIFEIHLQNSNNDNVSKVHVSQGNNKENNYQVAINTEDKRHLYDSRQIVVPQLKQMLSDFFTVRGSPFKQKGLKPSSPSDECDNPRDKEEKDWDLDRVTFDLGATREDCRETFSALCYLLLDCTTFPVYLSEEESELLYLSLFQVPGGSESSFPLWLKSLMTICCCVNDCYIQNLAISVLLEVINHSQSLALVIEDRIKRYKIPGQNPFFGRLQMVTIPPIAPGILKLISEKTDFYQRVAQVLWNQLNKETREHHNTCVELFYRLHCLAPSANICEDIICLALLDHDKCMRLEALFRFSVMWHLTREIQGNRTTSHNRSFDRSLFVVLDSLNYSDGAISAAAQGWLIRALSLNDVTRILEPALLLLLHPKTHRVSLHCIKQKNSEEDMSLWYTKNTFFQESVKSQVLCESSSEETLSLSQFTVVDREAIWAEVEKDPEKSLLKNEFFVNKPCLYAESLLEMGKDESEHTESADTTTGPLDSDNTSSFSHSPEQQDLIREENNSASTDGKNSMKHTGSSNVYPADSSKSSAVINLVRTDSDRTRASESLSSDDEMDLELQTLITSRLLKQQKEKQEMVEALFKHVLLYLQPYDAKRVLYAFSVLEGVLKTNPKEFIEAVVSTSMDTSSTAHLNLIYNLLARHQESLVGQSFYGKLQIQSPTMCPHSLLIELLMYLCLSFLRSYYPCYLKVTHKDVLGNRDVQVKSVEVLIRMVTQLTTMAKSTENRNIEFIRNLLGRCKIQEFVLLSISASMYISQKCYECMLANKTNGLSEHYILEESLINFGHDQIWSEQPLQIELLKLLQVLIVLEHHLGQTQEEQENQPALSKEWQRALSFQEAISAVQYVHPHPITSQGLFVSAVVRGLQPEYGYGMHPSWVGLVTYSLPYFGKSLGWTVAPFIVQICKNLDELVNQYEHEVLKMSTKISAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF09</accession>
    <name>A0A6I9YF09_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 13 member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC13A5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922741.1">
        <property type="nucleotide sequence ID" value="XM_014067266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF09"/>
    <dbReference type="GeneID" id="106549572"/>
    <dbReference type="KEGG" id="tsr:106549572"/>
    <dbReference type="CTD" id="284111"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017153">
        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015741">
        <property type="term" value="P:fumarate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015729">
        <property type="term" value="P:oxaloacetate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031312">
        <property type="entry name" value="Na/sul_symport_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF109">
        <property type="entry name" value="NA(+)_CITRATE COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01271">
        <property type="entry name" value="NA_SULFATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013922741.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="64887" checksum="9BC2009C4B9B320D" modified="2020-10-07" version="1">MAPPLPPFLRSLLRFKAFVTLFGTPLVLLPLPLLIGTQEAKCGYVIILMAVYWCTEVIPLAVTSLLPVILFPMFGILESKKVCMQYLKDTNMLFIGGLVVAIAVEHWNLHKRIALRVLLIVGVKPALLMLGFMGVTAFLSMWISNTATTAMMVPIIQAVLQQMNNSEHELAVMESTAGHINNAIELEGKVATGVSSVQGNGYLPDDSQEQDRIEKKRTNKGMTLCVCYAASIGGTATLTGTGPNLVLKGQMNQLFPRNDDIVNFASWFGFAFPNMLLMLALAWFWLQFSFIGFNFRKTWGCGSQKSEKEKAALNVLKEEYRKLGPISFAEISVLMNFTLLILLWFTRDPGFVPGWATFLFNKDKEYVTDATVAMFVALLLFILPSMRPRCGFRSQKSCDLEEAEEDMKEQFFPPPLLDWKTVQKKLPWNIVLLLGGGFALANGSDASGLSRWLGNQMTPLHTIPPWAIAVVLSIVIAIFTECTSNVATATLFLPVFASLSQSIEVNPLYIMIPATLSASFAFMLPVATPPNAIVFSYGHIRVSDMVKTGIVMNIIGIICVTLAINSWGRFMFQLDTFPSWANGTLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFF1</accession>
    <name>A0A6I9YFF1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ectonucleotide pyrophosphatase/phosphodiesterase family member 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013922739.1">
        <property type="nucleotide sequence ID" value="XM_014067264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFF1"/>
    <dbReference type="GeneID" id="106549570"/>
    <dbReference type="KEGG" id="tsr:106549570"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF65">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7-LIKE PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922739.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="44351" checksum="C8B3057C434A7D26" modified="2020-10-07" version="1">MDYLAKEGVKAKYITPPFVTMTSPSHFTMITGRWIEDHGVIHNMMFNTETLRKYGFKETQNKSEWWDNGVLPLWITAQNQGKKAASFHYPGGNANYSGQTVMRRLAESSNHPDSNETEWRENIDIVMNWFAKEDFDFVTLYYGEPDNIGHSVGPETEARKKIIRQIDRTLGYLLTSIKKHKLEDTLNVIITSDHGMTTVKKKPAVEEILLKDYIKFKDLVKFDILDYGGFGMILPKKGKEEEIYKKLKGAHPHLHVYKKEEFPEHFHYAKHERVLPILVYGDSGYAINGRFILYINKGDHGFDNEGMDMKTIFRAFGPDFKKGYLAEPFDSIHIYPLMCKLLGVQPELNNGSLALTEDMLRLSASGKRLVPLLGFGTIGLLFLLMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFH0</accession>
    <name>A0A6I9YFH0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106549775</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922988.1">
        <property type="nucleotide sequence ID" value="XM_014067513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFH0"/>
    <dbReference type="GeneID" id="106549775"/>
    <dbReference type="KEGG" id="tsr:106549775"/>
    <dbReference type="OrthoDB" id="5964498at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15229">
        <property type="entry name" value="7tmA_OR8S1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="36"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013922988.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="34238" checksum="C534EED02063D2AD" modified="2020-10-07" version="1">MENQTDFILLGLSSDPQLQIFFFLIFSIIFLITVSGNLTIILIIKIDPSLCVPMYYFLSHLSFVDMCYSTNIVPKMLMNFLMKQKTISFTFCILQMFCTLLLGVTEIFLLSAMAYDRYAAVCHPLHYIMIMNKAICTYLVLGAWLIGLLSATINTVPILKLHFCGNHEINHFSCELPPLLKASCSDIFLSNVILLSFAAICGLGSFLPTLVSYVHIISTIMKIHSAEGRSKAFSTCSSHLIVVGLQYVTGMSQYMKPSNVSSVTLDEIISVQYNILTPMLNPIIYSLKNKEVKRALKRLINPINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YFM9</accession>
    <name>A0A6I9YFM9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Probable G-protein coupled receptor 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GPR21</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923058.1">
        <property type="nucleotide sequence ID" value="XM_014067583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YFM9"/>
    <dbReference type="GeneID" id="106549832"/>
    <dbReference type="KEGG" id="tsr:106549832"/>
    <dbReference type="CTD" id="2844"/>
    <dbReference type="OrthoDB" id="6376512at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000177">
        <property type="entry name" value="probable G-protein coupled receptor 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF384">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="49"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013923058.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="39509" checksum="D1AEA36B961E3C4D" modified="2020-10-07" version="1">MNASLDGPGNGSSGGRPFCLLAAGYPDSLDVCLLEVIIIVFLTVLIISGNLVVIFVFHCAPLLNQHTTSYFIQTMAYADLLVGVSCLVPSLSLLHRPLAFLPESLVCKVFGYVVSVLKSVSMTSLACISLDRYIAITKPLTYSTLVTPCRLRSCILLLWLYSGAVFLPAFFEWGKPGYHGDIFQWCADYWDTRAVFTLFIVVMLYAPAALVVCFTYFSIFRICQQHTREINERRVRFSSQEGEPAEGQPCPDKRYAMVLLRITSVFYILWLPYIIYFLLESSSVYHNRLAAFLTTWLAISNSFCNCVIYSLSNSVFQKGLKHLSGALCTPCTRHRAGKDSSASRSKRPSNGCHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGC5</accession>
    <name>A0A6I9YGC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Voltage-dependent L-type calcium channel subunit alpha-1C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Voltage-gated calcium channel subunit alpha Cav1.2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="37" type="primary">LOC106549985</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm. Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm. Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Cell membrane</location>
            <location evidence="23">Sarcolemma</location>
            <location evidence="23">T-tubule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Membrane</location>
            <topology evidence="32">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Postsynaptic density membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013923227.1">
        <property type="nucleotide sequence ID" value="XM_014067752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGC5"/>
    <dbReference type="GeneID" id="106549985"/>
    <dbReference type="KEGG" id="tsr:106549985"/>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000007">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF10">
        <property type="entry name" value="VOLTAGE-DEPENDENT L-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 30 32" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 32" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="7 32" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19 31" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="30" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 34" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="13 32" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="35">
        <location>
            <begin position="43"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="35">
        <location>
            <begin position="256"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="33">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="31">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00024012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00024028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00024095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00030570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00045450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="RefSeq" id="XP_013923227.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="37727" checksum="AB348D9EB0D5D251" modified="2020-10-07" version="1">MGKMHKTCYLTGIATDTPAEEEPAPCAPVSQHGRQCQNGTDCRAIWEGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNRNTPADKKKGRFAWFSHSTETHVSMPTSETESVNTDNVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLAIASEHYNQPDWLTEVQDTANKVLLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGU6</accession>
    <name>A0A6I9YGU6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional adapter 2-alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TADA2A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013923219.1">
        <property type="nucleotide sequence ID" value="XM_014067744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGU6"/>
    <dbReference type="GeneID" id="106549980"/>
    <dbReference type="KEGG" id="tsr:106549980"/>
    <dbReference type="CTD" id="6871"/>
    <dbReference type="OrthoDB" id="270417at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional adapter 2-alpha/beta-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013923219.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="14914" checksum="9881FEC1A1AC2336" modified="2020-10-07" version="1">AIPLFFLICFPQEFDNYAEWDLRDIDFVEDDSDLLHALKIAVVDIYHSRLKERQRRKKIIKDHGLINLRKFQILERRYPKKIQDLYDSMRRFARILGPTEHDKFIESHAPWRLEEIDLATFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YHT1</accession>
    <name>A0A6I9YHT1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation-transporting ATPase</fullName>
            <ecNumber evidence="12">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ATP13A4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_013923746.1">
        <property type="nucleotide sequence ID" value="XM_014068271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YHT1"/>
    <dbReference type="GeneID" id="106550370"/>
    <dbReference type="KEGG" id="tsr:106550370"/>
    <dbReference type="CTD" id="84239"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07542">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047821">
        <property type="entry name" value="P5B-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF1">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE 13A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="830"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="855"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="895"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="956"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="986"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="17"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="164"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="214"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="677"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013923746.1"/>
        </source>
    </evidence>
    <sequence length="1094" mass="120624" checksum="A15699DA94577E7A" modified="2020-10-07" version="1">MGEKHGGSHRALLNEGEEAEVEIFGYKSRGLRRALCLAGYVLSCGGLLLLFHWKPQWGVWANCQPCSLEEADVVLLRTTDEFQICTRKNVTWIPLPAHLKPGSLPTDEEEPLLNRVIERPGFKVRSLQVQKIRYAWDFSAREFRKVGALEDHWSSLEVHARFGAGLSSSQQELRREICGPNTIDIEVTPIWKLLVKEQSVKLHQLVESHNSVLVKVFRKQTGTQEVESRHLVPGDTLILDEGRRLMPCDALLLSGQCIVNEGMLTGESLPITKTSLPQDCKSNQPWHLSSSEDSKRHLLFCGTDIIQAKGGDGGPARAVVLRTGFNTAKGDLVRSILYPKPMNFRLYRDAMQFLLCLVMLAAIGMVYAICVFVINGQEAGEVVKKALDVITIAVPPALPAALTTGIIYAQRRLKKKGVFCISPQRINVCGQLNLLCFDKTGTLTEDGLDLWGIVLAQGKSFQEVHRFSSGPPMPWGPAYAAMACCHSLMVLDGKIQGDPLDLKMFEATHWVPVKGLAVLRQFPFSSALQRMTVVAQEAPGDGQVFMKGAPEMVASFCQPETVPPSFARELQGCTSQGFRVIALAHKRLQEGRLAASMTREELESGLTFLGLLVMENRLKSQSRPVLQELSQAGLRSVMVTGDNLQTAITVARNAGMIPQGSRVVLVEARAWPGQPSASVSWEELEEPGSGPEVSGLAEGRQRGMGYPPPAGDLGWHFCLSGGSYEVLSQHFGHLLPQLLLNGTVFARMSPAQKAHLVQQFQQLDYYVGMCGDGANDCGALKEAHAGISLSQQAASVASPFTSQVPTIECVPELIRHGRAALVTSFCMFKYMALFSLASYIGVLLLYWQLNSFGNYQFLFQDLAITTLIGMTMSLNGPHPSLAPYRPPTQLIAPPLLLSVVLNLLLSLAVQLSGFLLVQQQLWYSASDIHSACSPGNATHGPQAPLPRQGFQSYENTSVWLLCTLYCLTVAVVFSKGRPFREPLYRNYVFVAVLMAQLAACLFFLFANIEGLYWGCSLLEWLIENRALWRWLKRAVGYQSSSRYKQLQRTLRQDPAWPPSNRTDFSGCTAVPLEGPGPTYTNPAFVSHEQPCHNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJD9</accession>
    <name>A0A6I9YJD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alpha-L-fucosidase</fullName>
            <ecNumber evidence="13">3.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">FUCA1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 13">Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a neolactoside IV(2)-alpha-Fuc-nLc4Cer(d18:0) + H2O = a neolactoside nLc4Cer(d18:0) + L-fucose</text>
            <dbReference type="Rhea" id="RHEA:49308"/>
            <dbReference type="ChEBI" id="CHEBI:2181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:91119"/>
            <dbReference type="ChEBI" id="CHEBI:91121"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:49309"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a neolactoside IV(2)-alpha-Fuc-nLc4Cer(d18:1(4E)) + H2O = a neolactoside nLc4Cer(d18:1(4E)) + L-fucose</text>
            <dbReference type="Rhea" id="RHEA:48224"/>
            <dbReference type="ChEBI" id="CHEBI:2181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17006"/>
            <dbReference type="ChEBI" id="CHEBI:28691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:48225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an alpha-L-fucoside + H2O = L-fucose + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:12288"/>
            <dbReference type="ChEBI" id="CHEBI:2181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28349"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="EC" id="3.2.1.51"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 13">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">Belongs to the glycosyl hydrolase 29 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.51" evidence="13"/>
    <dbReference type="RefSeq" id="XP_013924478.1">
        <property type="nucleotide sequence ID" value="XM_014069003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJD9"/>
    <dbReference type="GeneID" id="106551009"/>
    <dbReference type="KEGG" id="tsr:106551009"/>
    <dbReference type="CTD" id="2517"/>
    <dbReference type="OrthoDB" id="6039950at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004560">
        <property type="term" value="F:alpha-L-fucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000027">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016286">
        <property type="entry name" value="FUC_metazoa-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031919">
        <property type="entry name" value="Fucosidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000933">
        <property type="entry name" value="Glyco_hydro_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018526">
        <property type="entry name" value="Glyco_hydro_29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057739">
        <property type="entry name" value="Glyco_hydro_29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030:SF2">
        <property type="entry name" value="TISSUE ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01120">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16757">
        <property type="entry name" value="Fucosidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001092">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00741">
        <property type="entry name" value="GLHYDRLASE29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00812">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00385">
        <property type="entry name" value="ALPHA_L_FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027204048" description="Alpha-L-fucosidase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 29 N-terminal" evidence="15">
        <location>
            <begin position="29"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-fucosidase C-terminal" evidence="16">
        <location>
            <begin position="375"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="site" description="May be important for catalysis" evidence="14">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001092-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013924478.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="53406" checksum="4E5CA21A90253536" modified="2020-10-07" version="1">MAAERLRLLPPPLLLLLFFSCSGALTRNSAGPRYSPEWPSLDARPLPGWFDAAKVGVFVHWGVFSVPAWGSEWFWWHWKGELQPDYENFVRRHFPPGTSYADFAPRFSAVDFQPEEWAQLFQDAGARYVVFTSKHHEGFTNWGSEASWNWNSVDTGPHRDLVGELGEAVRKRNISFGLYHSLFEWFHPLYLLDKKNSFKSQFFVSEKTLPELYELVLRYKPDLIWSDGDWEAPDTYWNSTSFLAWLYNDSPVKDTVVVNDRWGANCSCKHGGYYNCQDKFEPSSLLDHKWEMCTSLDVRSWGYRSNMQVFDVMNGNTIIQQLVHTVSYGGNYLLNVGPTKEGQIAPIFQERLRALGLWLKVNGEAIYESKPWRTQKENRSEETWYTSKGPAVYAIFLVWPKDSVLTLSSPVVSASTQITMLGFSKPLKWQSSPQQELQVLLPSENRWLLPFEHGWAVKLLGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YK59</accession>
    <name>A0A6I9YK59_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Trinucleotide repeat-containing gene 18 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106550716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013924145.1">
        <property type="nucleotide sequence ID" value="XM_014068670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YK59"/>
    <dbReference type="GeneID" id="106550716"/>
    <dbReference type="KEGG" id="tsr:106550716"/>
    <dbReference type="OrthoDB" id="6426227at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052429">
        <property type="entry name" value="BAH_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12505">
        <property type="entry name" value="PHD FINGER TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12505:SF21">
        <property type="entry name" value="TRINUCLEOTIDE REPEAT-CONTAINING GENE 18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="528"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013924145.1"/>
        </source>
    </evidence>
    <sequence length="643" mass="70061" checksum="E8CDC52F1F5EBA84" modified="2020-10-07" version="1">TDGFYLAGHPGQAALHTQATPSRAAPNPASSTLLHEKDFGHPHRTPKDVPKGLGGKDRSYRSDLSFSLAKKEAKLREDSRPHSVVDLTQDTKPESERKTNGFEKAAKVGERLSPYLEEHLSHRGVFGGEPKSKHPLQTSSLSNCNMTGSAQGSELDRCPKDLSRHDENARPPPYVYSDRIKKVDAAMSSVAALHVACSCPSPAQPGKLPPPSAFPPQPIHPNMYTIFPLTKESGREHKVIAPTFVPSLEGYDERNGPIQIASQARDNAKLKEKEVPTAARVGVLQAVPERCLTEASRNLLAQEFSLHADAKRMEILREKSSVIRANSMVLKKQAASENFLNRPRLNSPESREYLPLKDLPKAGLEAEHRSCERDRFQRPGCKEAVKIYGTLDTSSPSSSASSLSSSSRQHLDPNQQLKPPEQKWKPFEMGNFATTQMAVLAAQHNHANRVEEDAKKTYLEPSNLQRSSVVGGRSVPESLHPPSHGEGSAMQSLIKYSGSFAKEASARQGGGKKSPFGGLGNMKLDPGQQPSPKLQQLLPHQAGKQLKKEPERPESAKSFGRESIGSQGEVEVRHLPVGIAVAVARQKDNSSGSKLGLVDRDRSLSLSNVKGRLWAGKHLALDGTVATPALTALVGEFWALRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKJ6</accession>
    <name>A0A6I9YKJ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Isoaspartyl peptidase/L-asparaginase</fullName>
            <ecNumber evidence="3">3.4.19.5</ecNumber>
            <ecNumber evidence="4">3.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Asparaginase-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Beta-aspartyl-peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Isoaspartyl dipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">L-asparagine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ASRGL1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.5" evidence="3"/>
    <dbReference type="EC" id="3.5.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013924757.1">
        <property type="nucleotide sequence ID" value="XM_014069282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKJ6"/>
    <dbReference type="GeneID" id="106551226"/>
    <dbReference type="KEGG" id="tsr:106551226"/>
    <dbReference type="CTD" id="80150"/>
    <dbReference type="OrthoDB" id="2262349at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033345">
        <property type="term" value="P:L-asparagine catabolic process via L-aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04702">
        <property type="entry name" value="ASRGL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033844">
        <property type="entry name" value="ASRGL1_meta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF41">
        <property type="entry name" value="ISOASPARTYL PEPTIDASE_L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="237"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="260"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="16">
        <location>
            <begin position="208"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013924757.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="37604" checksum="DDADB2522167CF8D" modified="2020-10-07" version="1">MCLPEAPSWLPRCPFRAASFKAFEAGGVWEQLKKSSSLKTFNSMKPVIVVHGGAGRIFKEREDGSRSGVIRAALKGYSILKQGGTALDAVEEAVISMEDDPHFNAGCGSVLNEKGEVEMDAIIMDGKNLASGAVSAVKCISNPIKLARLVMEKTEHMLLTDQGASAFAKAMGVPEVPGEKLITERSLERWKKNLEADWNPQECQKDLGTVGAVAIDSAGNVACATSTGGLSNKRLGRVGDTACIGSGGYADNSVGATSTTGHGESIMKVILARLTLYHMEQGKSPGEAADAALDCMKTRVGGLGGIIVISSSGEWAAKFSTNQMSWAVVKDDKLQSGIYTGERNTQSVEEALVSIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLM5</accession>
    <name>A0A6I9YLM5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PDZ domain-containing protein MAGIX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MAGIX</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925137.1">
        <property type="nucleotide sequence ID" value="XM_014069662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLM5"/>
    <dbReference type="GeneID" id="106551543"/>
    <dbReference type="KEGG" id="tsr:106551543"/>
    <dbReference type="CTD" id="79917"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000249">
        <property type="entry name" value="membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF41">
        <property type="entry name" value="MAGI FAMILY MEMBER, X-LINKED A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="100"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013925137.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="39150" checksum="A3887C26443FBFFB" modified="2020-10-07" version="1">MFEQVQTFLRHIQDFKPSSLGPHHKESPSHQEDAGLLARLTHGEVGSVFRQAGNRVRMKIRNRKEAGVSSDLNSADIEVGECPWSPTSHLTQPQESGQYSVELLRGPSGFGFSLRGGSEYNMDIYVLALMEGGPAQQCGKIQVSDQLVEINGESTAGMTHAQAVEHIRSGGSRIRLVLKRGNGFIPDYDRVFSQTATKTQQQVLEAEDTARHRHRKPNRSMVSHHVGQNLGDGDDREAAVCDVRQQLPRVHQSGQLHQTSSKAQGEDEEDREQGRRPHKQETNGESESDNHKLLSPRPSRKLRSDLVPGPWLVPSKERLSRALWGVCMGQGEEEVQKGNPGRGKGMEVKRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLX4</accession>
    <name>A0A6I9YLX4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myopalladin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106551616</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013925232.1">
        <property type="nucleotide sequence ID" value="XM_014069757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLX4"/>
    <dbReference type="GeneID" id="106551616"/>
    <dbReference type="KEGG" id="tsr:106551616"/>
    <dbReference type="OrthoDB" id="6612025at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="255"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013925232.1"/>
        </source>
    </evidence>
    <sequence length="287" mass="31950" checksum="E8F2006A0921A0E2" modified="2020-10-07" version="1">MQDNTSDSSTSITQLLRESYLAENSHQGNSERSRSESSLHSVHSGCASRASDEVTGQDELPDLSAFLSQEELDESVHLARQAINQEPPENSQEEEQQIHVFLPSELKNSENIISKKQQCQSTASPTSGNHPKKQAFGSETESKKELLNKAADFIEELSSLFKAHSSKRIRPKTCKNYRSKLESKHKANQDSLSENRERPCVPISQESDQIQPSLENPAYPQQADVEVVHTLETPVLTTETSAAPFYSEEPIGQPPHFTQKLKSREVSEGTKVQLDCIVVGIPTPEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YME0</accession>
    <name>A0A6I9YME0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P2Y purinoceptor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106551919</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925558.1">
        <property type="nucleotide sequence ID" value="XM_014070083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YME0"/>
    <dbReference type="GeneID" id="106551919"/>
    <dbReference type="KEGG" id="tsr:106551919"/>
    <dbReference type="OrthoDB" id="10018446at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031686">
        <property type="term" value="F:A1 adenosine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045028">
        <property type="term" value="F:G protein-coupled purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045030">
        <property type="term" value="F:G protein-coupled UTP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097746">
        <property type="term" value="P:blood vessel diameter maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070257">
        <property type="term" value="P:positive regulation of mucus secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15373">
        <property type="entry name" value="7tmA_P2Y2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000356">
        <property type="entry name" value="P2Y2_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF17">
        <property type="entry name" value="P2Y PURINOCEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00594">
        <property type="entry name" value="P2Y2PRNOCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="57"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013925558.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="41161" checksum="E7F9D709005F0481" modified="2020-10-07" version="1">MENVTFLMTSVNSCLNSSSEAGCGEEEHPYKCKFNEDFKYILLPVSYSIVFVVGLTLNLLALYVFLFRIKTWNTSTTYMFNLAVSDLLYVVSLPLLVYYYAMEDNWPFSVALCKIVRFLFYTNLYCSILFLLCISGHRFMGVCLPLRSLEWGQVRYARWVSAIVWLVVIACQSPVLFFVTTSQRGDSITCHDTSSKELFDQFLIYSSVMLALLFCIPFLLLIICYCMMARKLLQPTRGISRMSSSKKKSVKMILIVMLVFIICFLPFHITRTLYYSFRSWDLSCPTLDAINMAYKLTRPLASTNSCLDPILYFLAGQKFVKFACPQTAAKNGTESGTSTIPHSNFRGRDNVAMKTKHTAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMM2</accession>
    <name>A0A6I9YMM2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interactor protein for cytohesin exchange factors 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">IPCEF1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013925239.1">
        <property type="nucleotide sequence ID" value="XM_014069764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMM2"/>
    <dbReference type="GeneID" id="106551624"/>
    <dbReference type="KEGG" id="tsr:106551624"/>
    <dbReference type="CTD" id="26034"/>
    <dbReference type="OrthoDB" id="74412at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd01260">
        <property type="entry name" value="PH_CNK_mammalian-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051566">
        <property type="entry name" value="CNKSR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844:SF45">
        <property type="entry name" value="CNK3_IPCEF1 FUSION PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844">
        <property type="entry name" value="CONNECTOR ENCHANCER OF KINASE SUPPRESSOR OF RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="13"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013925239.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="46092" checksum="E5F50C4B19DC505A" modified="2020-10-07" version="1">MSRRRISCKDLGLADCQGWLYKKKEKGGFIGNKWKKFWCVLKASSLYWYSNQLAEKAEGFLNLPNFTVDQATECKKKHAMKITHPQIKTFYFAAENVHEMNRWLNKLGVAVLDQQANKKNEEECYSESEHEDPEINAETPPPPYSDQLLLSDLAQQELSSTSHFLNELPQSLSSPESSTMKSTGSSSSSLCNLLHSERQSWLDLINSSSPQEEVDNQPPAASGDSADTDCWLTDCNERVESGTNRSKENSPITVNEDKSSLGKANDNLAVPGKIAPATLSKDRTKCSVDEMEKLYKSLEQASLSPIGDRRPSTKKELRKSFIKRSKNPSVNEKLHKIRALNSTLKCKEHDLAKINQLLEDHKLTATKYREWKNTNSILIQDFYQPPDTMSTTDGDNTESEITPQSSFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YMR9</accession>
    <name>A0A6I9YMR9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-conjugating enzyme E2 G1</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E2 ubiquitin-conjugating enzyme G1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein G1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-protein ligase G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">UBE2G1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013925289.1">
        <property type="nucleotide sequence ID" value="XM_014069814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YMR9"/>
    <dbReference type="GeneID" id="106551672"/>
    <dbReference type="KEGG" id="tsr:106551672"/>
    <dbReference type="CTD" id="7326"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23795">
        <property type="entry name" value="UBCc_UBE2G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000018">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="14">
        <location>
            <begin position="14"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013925289.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="20520" checksum="98150592DB15EC99" modified="2020-10-07" version="1">MELLLKNIWTLHGAALEEYPDALLELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKYGYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKSQETAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPW8</accession>
    <name>A0A6I9YPW8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">26S protease regulatory subunit 10B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PSMC6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926558.1">
        <property type="nucleotide sequence ID" value="XM_014071083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPW8"/>
    <dbReference type="GeneID" id="106552734"/>
    <dbReference type="KEGG" id="tsr:106552734"/>
    <dbReference type="CTD" id="5706"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000008">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000034">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="101"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013926558.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36426" checksum="884713CD75C9796E" modified="2020-10-07" version="1">MCENCPAYNTSTCCGKRVIDLLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGDVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARAVASQLDCNFLKVVSSSIVDKYIGESARLIREMFNYARDHQPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLHRVKMIMATNRPDTLDPALLRPGRLDRKIHIDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEAGMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRH9</accession>
    <name>A0A6I9YRH9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Transmembrane protein 245</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">TMEM245</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926538.1">
        <property type="nucleotide sequence ID" value="XM_014071063.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013926539.1">
        <property type="nucleotide sequence ID" value="XM_014071064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRH9"/>
    <dbReference type="GeneID" id="106552722"/>
    <dbReference type="KEGG" id="tsr:106552722"/>
    <dbReference type="CTD" id="23731"/>
    <dbReference type="OrthoDB" id="5970161at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="586"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="615"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="779"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013926538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926539.1"/>
        </source>
    </evidence>
    <sequence length="812" mass="91605" checksum="9B0EF87BCDA39631" modified="2020-10-07" version="1">MAKYKRDGSSSSLSEPELDPDPQRYPPPPPPLQLPRLEKPLRQAFYNTGALVLVGLCCGVGVLIYFILEAFLRPLLWAVLCGTFLHPFKNSLTAFGRRWLRRLRHSNTPIFLATFLLPLSFANEAAETLGNEIVRRRRLLLLLSAGCPFLYGLYCVSTSSGVQVAVEYSASFICRGLDYFSSRWIWTLIAGYLLTVLFRWNQSTERYLRAISVPLWIVLLFHLASAAGSWRIPIVILIIVLMTVGLFHEQPNGKEPAEAQVLGQVISIAASAVTTVISDSCYESSNEDHPLQTSEIVPLSASANEKSRRRPEIAAFPKKKKASDIYFVLLVWSIVIVQIWFNFWIIQLLPLPFAVWVLKKLLVHFGVVQFSLTYCSSYWHVLQEFVRQRQEVLVPQPVKGLGRIILILDSKLIVWLEKILDKIISIFIIFVLVIGTLLLALLLTVQVHQESVHMIEVTSRLINETVAKHPEWANWLPEAQVIQEALNSAANNVYQYGREWITHKLDKILGEKVHNTAVIEKQVLELWDRLYHSWFAKNTTHSGQQKNHKKHLNREHSWTADMLDWQDVASFIHENIETFVSILESLWILMSHNVTLLFTTVTTLVTVLFHSSTAVLNFVLSLIIFLTTLFYLLSSSDEYYKPVKWVINLIPLLQSDASSNIVSESVEEAIRGVFDASLKMAGFYGLYTWLTHTIFGINIVFIPSALAAILGVVPFLGTYWASTPAVLDLWLVQGKEYKAMLLFICHLLPTYFVDTAIYLDISGGGHPYLTGLAIAGGTYYLGLEGAIIGPILLCIFMGAAKIYSAVLLSPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRN1</accession>
    <name>A0A6I9YRN1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat-containing protein 90-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106552760</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926589.1">
        <property type="nucleotide sequence ID" value="XM_014071114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRN1"/>
    <dbReference type="GeneID" id="106552760"/>
    <dbReference type="KEGG" id="tsr:106552760"/>
    <dbReference type="OrthoDB" id="6252103at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055441">
        <property type="entry name" value="Beta-prop_WDR90_POC16_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF24">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23393">
        <property type="entry name" value="Beta-prop_WDR90_POC16_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="268"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="WDR90/POC16 second beta-propeller" evidence="4">
        <location>
            <begin position="279"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013926589.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="57681" checksum="4BACAEC42E58FDA4" modified="2020-10-07" version="1">MGEMSVVRLWDFPSGKCLCVFKTGIHSISCLSFSYSGNVLCGTGKDKRGKTVVVVWNTVQVTRGGEVVMLAKAHTDVDIRAFKIAFFDDTRMVSCGRDNVRLWRIQSGALRSCPVNLGEYRSLEFTDLAFEVGHSTLREPEDRTLFVCSRSGHVLEIDYKNVTVKNVRRLLPLETGPVPQREKHAFNSGPGISLNSICFSSSFCATASDDGYLRLWPLDFSEVFLEAEHNNPVTSARISPDNLKVLCTTATGNVGCLDIQSRNYSTVMRSHMGTVLAFSVDSSQKQLATVSEDNTIRIWNVESMQQLYEFTASDEIPCAVAFHPLQPIFACGFDTGVVRTISLAASRLLEEHKQHRGLITGLTFSQDGNFMYSSCKHGTLSLYNCAIQKSHTVRVLANVVSQDNDHGPDALSVSCDGRLLAFVGPSKYIVTLMDAHTLDELLRIDVSILDLDSTALDVAMKLCFTPFPLGHLLVSTSSQKILVLDAKSGRLIRVVGDLQFLFSLSEVGLPCDFHVFPFTHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS66</accession>
    <name>A0A6I9YS66_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytokine-inducible SH2-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CISH</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927388.1">
        <property type="nucleotide sequence ID" value="XM_014071913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS66"/>
    <dbReference type="GeneID" id="106553420"/>
    <dbReference type="KEGG" id="tsr:106553420"/>
    <dbReference type="CTD" id="1154"/>
    <dbReference type="OrthoDB" id="10063348at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd10718">
        <property type="entry name" value="SH2_CIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000042">
        <property type="entry name" value="Cytokine-inducible SH2-containing protein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000002">
        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035887">
        <property type="entry name" value="CIS_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF9">
        <property type="entry name" value="CYTOKINE-INDUCIBLE SH2-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="93"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="209"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013927388.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="29330" checksum="3B6F16A77251E123" modified="2020-10-07" version="1">MLFPESWSDMILCVQGSYPLVSEEKLRRLPIEGLAVNVSEQVMQPLSVTSFQEQSAPPLVMPRLETNSPQTRDPEEDLLCIARTFSFLRESGWYWGSITANEAKQQLQKMPEGTFLVRDSAHPSYLFTLSVKTNRGPTNVRIEYADSKFQLDSNCLSKPRVLAFPDVVSLIQHYVLSCTVESKSDAPYPPPMPLPLPQKELIATAVHLKLIRALSRKDIAPSLQHLCRLQINRCTGKTDQLPLPKRMKDYLKQYPFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YS81</accession>
    <name>A0A6I9YS81_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC106553103</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106553103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927011.1">
        <property type="nucleotide sequence ID" value="XM_014071536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YS81"/>
    <dbReference type="GeneID" id="106553103"/>
    <dbReference type="KEGG" id="tsr:106553103"/>
    <dbReference type="OrthoDB" id="2386367at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052090">
        <property type="entry name" value="Cytolytic_pore-forming_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31594">
        <property type="entry name" value="AIG1-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31594:SF16">
        <property type="entry name" value="SI:CH211-281L24.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="42"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013927011.1"/>
        </source>
    </evidence>
    <sequence length="500" mass="56614" checksum="FDA0FEA38176542D" modified="2020-10-07" version="1">MFLLKDLPPNTEYQFQYAACCKVGHSKSNELSPPIKTLPTSPPEELRMVTAASSVISVAWMSPSIVASGVVVKEYKVEYRIVEAGSGKDQWREERTWRKTEFYLLEGLKPQTLTDVRIWVSAMCDNGALSGPSEEVEVSTSLEEESADNIAQQFIQINSLVEERQPLVFTLPLEKVSSDTSPSFLLYQLGQENLEVPNKVILVMGETGCGKITLINGMINYILGVQWKDYFRFKMIHETIQRSEAASRTSEVTAYVVNHQKGFQIPYSLTIIDTPGFGGARDSEQDNLVEKQLLEFFSTPGGIDHIDVICLLAQAFLAHSTHAQKCIFHSMLSMMGKDDNIQLLITFVDGWTPPVLETLNKADLPCAQNESGIPQHFRFNYLALFAPREYGGRHNAISEMFWKMSTETMKDFFDSLKMMEMNSLTLTMEVFKEIHLQRERRAEGEEPYHQVEGKICDFDHLKAFLGHSLQPEEVFEEDSVANVETALKMLAKWAMRGMNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YT97</accession>
    <name>A0A6I9YT97_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 2, facilitated glucose transporter member 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106553000</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926885.1">
        <property type="nucleotide sequence ID" value="XM_014071410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YT97"/>
    <dbReference type="GeneID" id="106553000"/>
    <dbReference type="KEGG" id="tsr:106553000"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005903">
        <property type="term" value="C:brush border"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF27">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013926885.1"/>
        </source>
    </evidence>
    <sequence length="235" mass="26316" checksum="DECD4F75293CCC0B" modified="2020-10-07" version="1">MDEGVIAREYDKGRLKEQGLTKISLCKCLICFYMYTDLLFSRIAKLKLTVQSTKLNSSSLSPHSPSLFQLFLVEKAGRRILFMAGLLGMMVCTITMTLGLVLQPKITWMSYVSLVSVFLFVSFFEIGPGPIPWFIVAELFSQGPRPAAIAIAGFSNWVTNFCIGMFFPYLAKLLGAYVFLVFAALLVLFVLFVYLKVPETKNKSFEEIAEEFRRRGKKDAGGPTEMEHLGAAEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTJ3</accession>
    <name>A0A6I9YTJ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DMBT1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106553830</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927878.1">
        <property type="nucleotide sequence ID" value="XM_014072403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTJ3"/>
    <dbReference type="GeneID" id="106553830"/>
    <dbReference type="KEGG" id="tsr:106553830"/>
    <dbReference type="OrthoDB" id="10063988at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052129">
        <property type="entry name" value="Spermadhesin-Link_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46908">
        <property type="entry name" value="CUBILIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46908:SF4">
        <property type="entry name" value="TUMOR NECROSIS FACTOR-INDUCIBLE GENE 6 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026729321" evidence="3">
        <location>
            <begin position="23"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="38"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013927878.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="27575" checksum="7B558508534BA0F8" modified="2020-10-07" version="1">MDHTLIFLWVLLLSATASTVSGVTPLPSDVSATAEPACGGSFSDASGSFFGPYYAAGNRMLTCVWRINSPEYYPIRINLDYINLDCASEYIAVYKGEPQTSTLLGKICEGEKTFYSYSGMMTLVLYRHSNTEGQGFIAFYDVGAAYTTPLPIADLTTRLEETSGIGVIGETSALPTSPSRTAIILTHSSEKATSKKIHTFIFYNHALMSDEKFSLVIVILTSLIALHVSRNNGRNIRGFKEVLEPVREVILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YU31</accession>
    <name>A0A6I9YU31_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase-like protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106553772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927812.1">
        <property type="nucleotide sequence ID" value="XM_014072337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YU31"/>
    <dbReference type="GeneID" id="106553772"/>
    <dbReference type="KEGG" id="tsr:106553772"/>
    <dbReference type="OrthoDB" id="61116at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF4">
        <property type="entry name" value="N(6)-ADENINE-SPECIFIC METHYLTRANSFERASE METTL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013927812.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="45934" checksum="B574A03FDE630C30" modified="2020-10-07" version="1">MVKRTYLFREEFFNVIKPHIPASFQTEKHSGINLETSISEQQKNDKDKKSVITVKAKKKRKRSSELNHGESDILEYHLKISDIILDGTRCLIQEARRSGFLQPNSIDQFYVKASDRGPNNCSLAELCEMAKCFVPVNEKEQICVMEEETSDPEQVQLTCVTENNTSNVKMLTLMGQIYLFPPRSAFLLSDFSCMQPLLNYKKKYDIIVIDPPWENKSVKRSNRYGFLSHWQIKEIPVPALAAPNCLVVMWVTNRQKHLQFVKNELYPHWSIHGVVEWFWVKITKSGEFVIPLDSLHKKPYEILVLGRVQGNTDVPLRLSEDEKFPAIPGQKLIVSVPCTLHSHKPPLSEILKEYTKPDVECLELFARNLQPGWVSWGNEVLKFQHLDYFTVLQDNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUV3</accession>
    <name>A0A6I9YUV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosyl-DNA phosphodiesterase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">5'-tyrosyl-DNA phosphodiesterase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106553747</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927791.1">
        <property type="nucleotide sequence ID" value="XM_014072316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUV3"/>
    <dbReference type="GeneID" id="106553747"/>
    <dbReference type="KEGG" id="tsr:106553747"/>
    <dbReference type="OrthoDB" id="9975959at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070260">
        <property type="term" value="F:5'-tyrosyl-DNA phosphodiesterase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09080">
        <property type="entry name" value="TDP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000024">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051547">
        <property type="entry name" value="TDP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822">
        <property type="entry name" value="TRAF AND TNF RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="14">
        <location>
            <begin position="63"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017870"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013927791.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="29946" checksum="2D3448D3840D6D44" modified="2020-10-07" version="1">MQKALNSYFDPSPEQDVDRMSHPVPATGAPVELTERSTTNTVAAAVVEAAGICPQDDSTFSLITWNIDGLDSNNLQERARGVCSYLALYSPDVVFLQEVIPVYLSYLQKRAVSYTIIPGNTDGYFTAIMLKKSRVKVLKHEITAFPTTSMLRNLLAVYVNISGNDLCLMTSHLESTKDHSEERLKQLKLVLAKIAEVPESSSVIFGGDTNLRDKEVAKIGGLPNNVLDIWEFLGKPEHCKYTWDTTQNSNLNVRYKCKSRFDRLFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YV35</accession>
    <name>A0A6I9YV35_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Sushi, nidogen and EGF-like domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SNED1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927866.1">
        <property type="nucleotide sequence ID" value="XM_014072391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YV35"/>
    <dbReference type="GeneID" id="106553820"/>
    <dbReference type="KEGG" id="tsr:106553820"/>
    <dbReference type="CTD" id="25992"/>
    <dbReference type="OrthoDB" id="9972657at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000537">
        <property type="entry name" value="Notch 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000057">
        <property type="entry name" value="protocadherin Fat 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000360">
        <property type="entry name" value="Sushi, nidogen and EGF like domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000213">
        <property type="entry name" value="sushi, nidogen and EGF-like domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000239">
        <property type="entry name" value="Sushi, nidogen and EGF-like domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000251">
        <property type="entry name" value="sushi, nidogen and EGF-like domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000373">
        <property type="entry name" value="sushi, nidogen and EGF-like domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000255">
        <property type="entry name" value="Sushi, nidogen and EGF-like domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000309">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF22">
        <property type="entry name" value="DROSOPHILA CRUMBS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7 15" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="NIDO" evidence="18">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="72"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="115"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="191"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="229"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="272"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="311"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="385"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="424"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="463"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="501"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="17">
        <location>
            <begin position="540"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="597"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013927866.1"/>
        </source>
    </evidence>
    <sequence length="643" mass="69130" checksum="C75C5B3834FD1090" modified="2020-10-07" version="1">MHASSGGNLAGLGGIAAQAGFNAGDGKRYFNIPGSRTDDIVDVEMTTNVGIPGRWVFRIDDAQVQVGGCNNTTSVCLTLRPCLNGGKCIEDCITGNPSYTCSCLAGFTGKQCQIDVDECASNPCHNKASCISGINSFSCWCPPGFKGATCEIEESPCKTKECQNGGECQITNTTAVCLCQPGYTGEDCEIEINECESSPCLNGGQCMDLVNNFTCSCATPFTGQHCEIDPSTCEGRNCRNRQMCNYIRPGRYVCTCSPGYYGNNCQYGGPRMSNACSSNPCQNEGTCLESSQGYVCECAEGYAGTDCRDKVSDDCECRNGGKCLDGNITVCHCPPGYFGLLCEFEVTTTPCNMNTQCPDGGYCMEYGGSYLCVCHTDYGTNHTEPPSPCDSEPCLNGGSCDAHEDSYTCDCPRGFTGKYCEKERPKLCSSGPCRNGGTCKESDGEYHCTCPYRFTGKHCEIGKPDPCTSGPCHNGGTCFHYIGKYKCDCLPGYTGRHCETAPSPCFRHPCKNGATCEDFGNSYVCICSAGYTGRHCQSEIDCGIPTEVKHAQLAFNSTKMGSLAIYHCAFGYILNSQNNVRLCLAKGIWSDPPECNEIDECTSQPCLNGGLCKDQIAHYLCLCKAGYTGNNCESGKRTWKHLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YVA8</accession>
    <name>A0A6I9YVA8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dual specificity protein phosphatase 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DUSP18</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 18">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 18">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927944.1">
        <property type="nucleotide sequence ID" value="XM_014072469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YVA8"/>
    <dbReference type="GeneID" id="106553886"/>
    <dbReference type="KEGG" id="tsr:106553886"/>
    <dbReference type="CTD" id="150290"/>
    <dbReference type="OrthoDB" id="285418at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000049">
        <property type="entry name" value="Dual specificity protein phosphatase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020420">
        <property type="entry name" value="Atypical_DUSP_subfamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46495:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46495">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01910">
        <property type="entry name" value="ADSPHPHTASEB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="80"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013927944.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="21425" checksum="6776F382CA1B686C" modified="2020-10-07" version="1">MNAVFGAIPILFRNPSVYGLSRITNSLFISNGEAANNKLFLYANRITTVINATVEVVNTYFPDIYYIHVPVLDYPSARIYDFFDPVADKIQAVDRNQGRTLVHCAAGVSRSAALCIAYLMKYHSMSLYNAHDWVKSCRPVIRPNLGFWEQLVEYEYHLFGKNSVKMIQSPIGRIPDLYESEVRVMISM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWE6</accession>
    <name>A0A6I9YWE6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ferric-chelate reductase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">FRRS1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928973.1">
        <property type="nucleotide sequence ID" value="XM_014073498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWE6"/>
    <dbReference type="GeneID" id="106554763"/>
    <dbReference type="KEGG" id="tsr:106554763"/>
    <dbReference type="CTD" id="391059"/>
    <dbReference type="OrthoDB" id="6372137at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016722">
        <property type="term" value="F:oxidoreductase activity, acting on metal ions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08544">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4060">
        <property type="entry name" value="Reeler domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051237">
        <property type="entry name" value="Ferric-chelate_Red/DefProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002861">
        <property type="entry name" value="Reeler_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042307">
        <property type="entry name" value="Reeler_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45828">
        <property type="entry name" value="CYTOCHROME B561/FERRIC REDUCTASE TRANSMEMBRANE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45828:SF3">
        <property type="entry name" value="FERRIC-CHELATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02014">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51019">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Reelin" evidence="11">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="10">
        <location>
            <begin position="261"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928973.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="44407" checksum="D44EB80AD90F80F6" modified="2020-10-07" version="1">MECQAAFEKLKRLFAEEPVLKHPDMDAAFVVQADASDVAVGAMLLQANGQVSFSGPTFQGFLLEARNADRLDDPPIGSFALADRRQSQLLRCGHVKNSAVSHTSKSKKNHMDAYWIAPSDAPKKVQFLLTIVQKYIIYWVKIPGPKISQAGILPFTTIMFTNWTIPASLPVSSLSQPGGGDDAYFCISEVYSVNMRTAALVGRSYPDFNSKNALENITWRLADGLIQCAFRRKIHLPASTGRYNLDANYYIFLADGEISTEGEIYKHQQQPLITNGKYNILGPLKDIGGSRSPFLIKMHGALMFVGWITAVSIGVIVARFFKSVWPHTLLFGEEIWFQIHRSLMMITVLLTSISFVLPFIYRGGWNKQAGFHSYFGSTVMALALFQPLMAAFRPPQQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWS8</accession>
    <name>A0A6I9YWS8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TM9SF2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928426.1">
        <property type="nucleotide sequence ID" value="XM_014072951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWS8"/>
    <dbReference type="GeneID" id="106554298"/>
    <dbReference type="KEGG" id="tsr:106554298"/>
    <dbReference type="CTD" id="9375"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027140187" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="23"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="451"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="541"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="612"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013928426.1"/>
        </source>
    </evidence>
    <sequence length="651" mass="74555" checksum="958400AB7DB22534" modified="2020-10-07" version="1">MAVARLLLLSALQAVLPPFAFAFYLPGLAPVNFCEKSDKVSECKSVIELFVNRLDSVESVLPYEYTAFDFCQAEGGKRPSENLGQVLFGERIEPSPYKFIFNQKETCRHVCTKEYDLNKPEQRQKLQFLKKSMLLNYQHHWIVDNMPVTWCYEVEDNQKFCNPGFPIGCYVTKDGHARGACVISSEFHDSDTFYVFNHVDIKIVYHVVENEAPRARLVAAKLEPKSFRHTNVDKPDCSGLPMDLRNTINDGIIKIAYTYSISFEENKEIRWASRWDYILESMSHTHIQWFSIMNSLVIVLFLSGMVAMIMLRTLHKDIARYNQMDSTEDAQEEFGWKLVHGDIFRPPRKGMLLSVFLGSGTQILIMTFVTLFFACLGFLSPANRGALMTCAVVLWVLLGTPAGYVAARFYKSFGGEKWKTNVLLTAFLCPGIVFADFFIMNLILWGEGSSAAIPFGTLVAVLALWFCISVPLTFIGAYFGFTKNAIEHPVRTNQIPRQIPEQSFYTKPLPGIVMGGILPFGCIFIQLFFILNSIWSHQMYYMFGFLFLVFIILVITCSEATILLCYFHLCAEDYHWQWRSFLTSGFTAFYFLIYAVHYFFSKLQITGTASTILYFGYTMIMVLIFFLFTGTIGFFACFWFVTKIYSVVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWY7</accession>
    <name>A0A6I9YWY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Laminin subunit beta-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Laminin B1 chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Laminin-1 subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Laminin-10 subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Laminin-12 subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Laminin-2 subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Laminin-6 subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Laminin-8 subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC106554044</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-1 is a subunit of laminin-1 (laminin-111 or EHS laminin), laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213). Interacts with ITGB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928120.1">
        <property type="nucleotide sequence ID" value="XM_014072645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWY7"/>
    <dbReference type="GeneID" id="106554044"/>
    <dbReference type="KEGG" id="tsr:106554044"/>
    <dbReference type="OrthoDB" id="5985440at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005606">
        <property type="term" value="C:laminin-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043259">
        <property type="term" value="C:laminin-10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000083">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000084">
        <property type="entry name" value="Laminin subunit alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000065">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000101">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000130">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000138">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000145">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000004">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000135">
        <property type="entry name" value="Laminin subunit beta 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000280">
        <property type="entry name" value="Laminin subunit beta 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013015">
        <property type="entry name" value="Laminin_IV_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF233">
        <property type="entry name" value="LAMININ SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21199">
        <property type="entry name" value="LAMININ_IV_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51116">
        <property type="entry name" value="LAMININ_IVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 22" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin EGF-like" evidence="24">
        <location>
            <begin position="86"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="24">
        <location>
            <begin position="146"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type B" evidence="25">
        <location>
            <begin position="187"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="24">
        <location>
            <begin position="198"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="24">
        <location>
            <begin position="403"/>
            <end position="450"/>
        </location>
    </feature>
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            <end position="496"/>
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            <begin position="875"/>
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            <begin position="116"/>
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    <sequence length="1040" mass="114447" checksum="ADC84C5F2841DAA4" modified="2020-10-07" version="1">VIYRVLDPAIPIHDPYSAKIQKCNCNEHSHRCHFDMAAYLATGNVSGGVCDQCQHNTMGHHCQFCKPFYYKDPTKDIRDPGMCRACDCDPDGTLDGGVCDTHDDPMLRLIAGQCRCKEHVEGARCDRCKSGFSGLSAKNPQGCQRCQCDPRGTVQDGPQCDAITGDCFCKRLVSGRSCNQCLPEHWALSLDLYGCRPCDCDVGGAYDNQCARETGQCRCRSHMVGRQCNQVQTGYYRIVLDHYTYEAEEARLHQFAGKWEKVKVSVVRPGPVPSGSPCGNIIPADDLISTALLPTSRYIVLSQPVCLEQGIRYTIRVEFTHYGGREHLKGAAVLLDSLVLVPRYSSLEMFIAGDAASIARKETFERYRCAQQAHPVIKGPTPEACAKLLTSMSAIIHQGALPCQCDLQGSLSLECNPSGGQCQCKPHVMGRRCERCAPGTFGFGPGGCKSCQCSSQGSQSSFCDNFSGQCPCRAGVFGLRCDHCQAGHWGFPNCRPCQCNGRAEECEQRTGSCLHCRGHTEGNWCERCVSGYYGNPALSLGGQCRPCPCPEAPGSGRNFAVTCHQDNPSGTVRCSCLGGYTGPRCDECAPGYYGNPWQVRGRCQPCTCNNNIDLADPKSCDRRTGQCLRCLYHSEGDQCQHCQRGYYGDATQHTCRRCSCNYLGTVQNKCSSQDQCQCDRHSGQCQCLPNVQGQNCDRCAPNFWNLTSGQGCQPCDCHSYYSLSPACNQFTGQCQCRPGFGGRTCSECQENHWGQPGQQCRACDCDPRGIEKPQCHRTTGHCSCRPGVSGVRCDQCSRGFAGDFPACQPCHQCFGDWDRIVQDLAAHTRNLMQRAQQLHQTGLAGAFEGPFRRLQDKLSAIQAVVSARNATATAIAHLLHNMDDIRQEIADITETLTQLEGLLTATQDKNFNANHGLGILERGVRSLNLSLADLKHQLNILINTNVLGVYDSVRQSFVQSREAEHQANASTLAVPSPVSQSAATRRRTEHLMMSRREAFNRQNAANRRGLVDLSTRTRGLSLDLINEKVREHENRMFLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXW9</accession>
    <name>A0A6I9YXW9_9SAUR</name>
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        <submittedName>
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        <name evidence="7" type="primary">LOC106554687</name>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RFWD3-RELATED"/>
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            <begin position="7"/>
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    <sequence length="108" mass="12365" checksum="4031147DE8821E4B" modified="2020-10-07" version="1">MPIRAHCTICSDFFDNKTDVAAIHCGHTFHHLCLIQWFDTAPSRTCPQCRIQNELDRVKAQLSMKEKEKRDCQSIVNALRETLDLRNATVESLQKAISDTEMLCSTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY28</accession>
    <name>A0A6I9YY28_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Testis-expressed sequence 9 protein-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC106554979</name>
    </gene>
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        <name type="scientific">Thamnophis sirtalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013929207.1">
        <property type="nucleotide sequence ID" value="XM_014073732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY28"/>
    <dbReference type="GeneID" id="106554979"/>
    <dbReference type="KEGG" id="tsr:106554979"/>
    <dbReference type="OrthoDB" id="269872at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313:SF0">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313">
        <property type="entry name" value="TSEC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013929207.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="42263" checksum="26032AAA21987FF2" modified="2020-10-07" version="1">MAAAGEPSGEQPARPRSAAPLLAREEEYRRLNAALEAKAAAVMRQAEELMRDRREALARPVSARGDAPEEEAAAKERDINSPEPAVSFAQNKQLPKKKASTAPTIRNRPLTGRKGKRPLLSTKSRLQVDPSNTDVAAPGCSLTKLIGKIEGQLEEGNLPDLEDNLIPSSGCELGAEAQIRFLKAKLRVMQEELDAVGQECSKKEGENENLSSRLKEIEEERGRLQRAASLHQSQAEKYRLLSEEATRKSEALQQKLSTLEKELENLKRAQKQAAATQSTTDIRLNRALEEAEKYKMELNKLKQSNKDVVNQEHKKLEELKIENKRLEKQKEELVAGFKKQLKLIDILKRQKMHIESAKMLSFTEEEFMKALEWGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY41</accession>
    <name>A0A6I9YY41_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium-coupled neutral amino acid transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC38A1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929222.1">
        <property type="nucleotide sequence ID" value="XM_014073747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY41"/>
    <dbReference type="GeneID" id="106554987"/>
    <dbReference type="KEGG" id="tsr:106554987"/>
    <dbReference type="CTD" id="81539"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF184">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID SYMPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="15"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013929222.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="34246" checksum="2F2F29E025FF514C" modified="2020-10-07" version="1">MGEGDTFVAWYVDGRVLVVAVTFLIILPLCLLKNLGYLGYTSGFSLTCMVFFLIVVIYKKFQLSCPFLDRNATDATFSNFSFGTEGCRPKYVTFNSKSVYALPTIAFAFVCHPSVLPIYSELKDPSQKRMQMVTKISFFAMFVMYFLTAIFGYLTFYESVQSDLLHKYQNKDDILVLTVRVAVIIAVILTVPVLFFTVRSSIFQLARKNKFHLCQHIVVTLVLLMIINLLVIFIPSMKDIFGVVGTTSANMLIFILPSSLYLKITHQDGSKLIQRIWASLFLALGILFSMVSIPLVIYDWVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YY94</accession>
    <name>A0A6I9YY94_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine rich adaptor protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106554701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013928901.1">
        <property type="nucleotide sequence ID" value="XM_014073426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YY94"/>
    <dbReference type="GeneID" id="106554701"/>
    <dbReference type="KEGG" id="tsr:106554701"/>
    <dbReference type="OrthoDB" id="8911462at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039499">
        <property type="entry name" value="LURA1/LRA25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037443">
        <property type="entry name" value="LURAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33767:SF2">
        <property type="entry name" value="LEUCINE RICH ADAPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33767">
        <property type="entry name" value="LEUCINE RICH ADAPTOR PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14854">
        <property type="entry name" value="LURAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013928901.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29144" checksum="956C38376DECE428" modified="2020-10-07" version="1">MSLLSTKAHSNWEKCQILKNKQAYLRAIDIKILHQLVAVNDGIETVKWFLEEKGMLTSHCSSLTSSQYSLVESQDTSRRGSWNSLQDPNDKLDSISIGSYLDTLADDMDEYGQSSSVEPIMSPTLGRPLGRSELERSKADLERIFLAKMDKEQDKSKTYPEWASSLVKSQSFNKPSAPVQLSPPMANGILGRQVPKLVPSLEEKFTSAAAEQPNKESVAVKASRKSKMDPENCKLDNRKHLEYDGQWQWAESQEDVTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YYH9</accession>
    <name>A0A6I9YYH9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TSPAN12</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928981.1">
        <property type="nucleotide sequence ID" value="XM_014073506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YYH9"/>
    <dbReference type="GeneID" id="106554775"/>
    <dbReference type="KEGG" id="tsr:106554775"/>
    <dbReference type="CTD" id="23554"/>
    <dbReference type="OrthoDB" id="8813994at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03157">
        <property type="entry name" value="TM4SF12_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000009">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF462">
        <property type="entry name" value="TETRASPANIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928981.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="35533" checksum="7BC1473E712CBCF2" modified="2020-10-07" version="1">MAREDSVKCLRCLLYALNLLFWLMSITVLCVSAWMRDYLNNVLTLTAETRVEEAVVSTYFPVVHPVMIAVCCFLIIIGMLGYCGTVKRNLLLLVWYFGSLLVIFCIELACGVWTYEQEITVPVQWSDMVTLKARMINYGLPRYHWLTHAWNFFQREFKCCGVVYFTDWLEMTEMDWPPDSCCVREFPGCSKQAHHEDLSDLYQEGCDKKMYTFLRGTKQLQVLRFLGISIGVTQILAMILTITLVWALYYDRQDPRSDQMLSLRKDASQHLSCHSVELLKPSLTRILEHTTMANSFNTHFEMEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZN2</accession>
    <name>A0A6I9YZN2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="11">1.1.99.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">D2HGDH</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-malate + A = oxaloacetate + AH2</text>
            <dbReference type="Rhea" id="RHEA:67460"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67461"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.39" evidence="11"/>
    <dbReference type="RefSeq" id="XP_013929499.1">
        <property type="nucleotide sequence ID" value="XM_014074024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZN2"/>
    <dbReference type="GeneID" id="106555203"/>
    <dbReference type="KEGG" id="tsr:106555203"/>
    <dbReference type="CTD" id="728294"/>
    <dbReference type="OrthoDB" id="5332616at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051990">
        <property type="term" value="F:(R)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2190:FF:000001">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000053">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051264">
        <property type="entry name" value="FAD-oxidored/transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716:SF1">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="15">
        <location>
            <begin position="132"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013929499.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="61897" checksum="A0F44D4A5BD59BDA" modified="2020-10-07" version="1">MAISSLVWPCLFQYMGTVPRLVTRCQRKTRSIWNRTATLSRIEKKATQPLKTLLLLPSLQWSISNIAKRNLHTSQLHLQEVMLTCERYGVERLPFAHISDSDLTFFEHLLPGRVITDAEELKPFNVDWLKSVRGCSTVLLKPRTTMEVSQILKYCNEKNLAVNPQGGNTGLVGGSVPVFDEIILSTGLMNQIISFDHISGILISQAGCILENLNQYLEPKGFVMPLDLGAKGSCHIGGNIATNAGGLRLLRYGSLRGTVLGLEVVLADGSILNCLASLRKDNTGYDLKQLFIGSEGTLGMVTAVSIICPRKSNAVNLAFLACPSFSQVLRTFIMCKDMLGEILSAYEFMDNGCMKLVERHLKLSNPVTESPFYVLIETSGSNSTHDEEKLSHFLEHVMGSDLVVDGTLASEGKKIKALWALRERITEALTCDGVVYKYDISLPVEKLYDLVTDMKVRLGTAAKNVVGYGHLGDGNLHLNVTAESYSHSLLDAIEPYVYEWVSQYYGSISAEHGLGFKKKHYIHYSKPKEAILLMQQFKVMLDPKGILNPYKTLPSKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0I0</accession>
    <name>A0A6I9Z0I0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106555463</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013929794.1">
        <property type="nucleotide sequence ID" value="XM_014074319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z0I0"/>
    <dbReference type="GeneID" id="106555463"/>
    <dbReference type="KEGG" id="tsr:106555463"/>
    <dbReference type="OrthoDB" id="8898434at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000001">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000153">
        <property type="entry name" value="Zinc finger RNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000210">
        <property type="entry name" value="Zinc finger RNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000010">
        <property type="entry name" value="Zinc finger RNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006561">
        <property type="entry name" value="DZF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049402">
        <property type="entry name" value="DZF_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049401">
        <property type="entry name" value="DZF_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762:SF21">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20965">
        <property type="entry name" value="DZF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
        <property type="entry name" value="DZF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00572">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51703">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DZF" evidence="11">
        <location>
            <begin position="366"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="427"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013929794.1"/>
        </source>
    </evidence>
    <sequence length="750" mass="83837" checksum="78C5D103E34CC66A" modified="2020-10-07" version="1">DVCKISCAGPQTYKEHLEGQKHKKKEAALKVSQNTTSNSASSRGTQNQLRCELCDVSCTGADAYAAHIRGAKHQKVVKLHTKLGKPIPSTEPNVVTQATSATSSSKPASVSSNIATSSNTVNSSVTSLAVKILTPSANVLLSTTSNSKSTIVPTNIAVKKISTPKINFVGGNKLQTTGSKADEMKAVENIKTTPIATVQMQETKPDPSAEPVTSTALAALQSEVQPVGHDYVEEVRNDEGKVIRFHCKLCECSFNDPNAKEMHLKGRRHRLQYKKKVNPDLQVEVKPSIRARKIQEEKMRKQMQKEEYWRRREEEERWRMEMRRYEEDMYWRRMEDEQHHWDDRRRIPDGGYPHGPPGPLGLLGVRPGMPPQPQGPAPLRRPDSSDDRYVMTKHAAIYPTEEELQAVQKIVSITERALKLVSDNMTDTEKNKTKEGDDKKEGNKDRALKGVLRVGVLAKGLLLRGDRNVNLVLLCAEKPTKSLLSRIGESLPKQLAVISPEKYEIKCAVQEAAIILNSCVEPKMQVTITLTSPVIREENMRDGDVTSGMVKDPPDVLDRQKCLDALAALRHAKWFQARANGLQSCVIIIRILRDLCQRVPTWSDFPSWAMELLVEKAISSATGPQSPGDALRRVFECISSGIILKGGPGLLDPCEKDPFDTLALMTDQQREDITSSAQFALRLLAFRQIHKVLGMDPLPQMNQRFNIHSNRKRRRDSDGLMDLKLRGKKTRKTMITFKEDICVCIVNTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z0X4</accession>
    <name>A0A6I9Z0X4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable E3 ubiquitin-protein ligase HECTD4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106555405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929726.1">
        <property type="nucleotide sequence ID" value="XM_014074251.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106555405"/>
    <dbReference type="KEGG" id="tsr:106555405"/>
    <dbReference type="OrthoDB" id="5986060at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043366">
        <property type="entry name" value="HECTD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46435">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46435:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="5">
        <location>
            <begin position="1271"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="651"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="961"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="493"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="961"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1004"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929726.1"/>
        </source>
    </evidence>
    <sequence length="1446" mass="158398" checksum="66D92EB278EF1FA6" modified="2020-10-07" version="1">MLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPSGYRRTATNGLVSLDNSNIQIHRELLRCEAMLACLYCRMALLNIFAPKTPHLFTQLFSVRDITLEHLQLLSNQLLAPPLPDGTISSTSILLAQSLQHSIHSQSCSATELFYQGNAQTVQEWLTIAIARTLHQGEESLLELTRQACYFLQNAPDQFPSEEFPISESKVNMDVHFPGAAFVIVSCKESQSGFRKDSSLYKAPWARILLYGLGHKVKKNGQLNLIEAVCYPRDASPTNTGLTPPPTTNQYPSVIISTDKVHIKLGVSPPPGAVLVLHSLPLEFPLAVAFAEQLLTWKQEEGEGRSDDEPDAIPASVFLQVVEILGNFLWTTSMAPCVKELLFHLLAELLRKVHRLEQRKSPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGGNACGGAGVGGADQGRFSTYFHALMEACLAVAEVTLPVNMGITANVMSSTSGPNLSDSSSSSSSSPPGQTPQSPSLLSKRKKVKMKREKATPSGKRSSSRAADTDPALLSMGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTNDAVADACQALVGATAHSRLLVISGIPTHLEEGVVRSAIRKACNAHGGVFRDEIYIPVQEEQPKKAKDKAEGGECRTELEKPAVSSSVDSLDISSSSSVTPAMSVSASASTSQASLCSSQGISRTVSDISVDQFQGGLEVAVPPGLLEPHGVSSQESLDLSLCSTGSLGSLGSLGEQLDNGETASASDVGSLYTVTSLDSQTVPSRPIKGFAVVEIRSRAKIEKIRASLFNSSDLIGLSALDGEEELMEMSSEEILTVSTVNQSLFDTQGSPALEDYFSDKTLKGDKLVPGAREVLTDIFKSCLHSEQMLSLLPAKPTKVMEIYLSKEQINTQAPGNLLHTFFTHVRPPKKALEDQLTQILRKYGVPKPKFDKSKYSKGGKEQHPVKVVSTKRPATKPPAKEKAVLNSVSRTALSEKKPTVKARSPEKGKPEEKDPDKSPTKKQEAPEEKYLTLEGFHRFVIDRAKQDIRSVWRAILSCGYDLHFERCSCLDARNAQKACRKWSLEMDVALVRYINRLCRHLAITPARLHPHEVYLDSADAADPQVSCLLNTPIESLRLRFALLQSLNTTLETFFLPLVELRQTKVYPNSIAALLQEAKGLIFYDTKVTVLNRVLNATVQRTADHAAPEITLDPLEIVGGEIRSSENSYFCQAARQLACIPSSQLCVKLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSTLLSLLLLCPSSSVNKNKGKYILTPSPISYGEEQLLHFFGQLLGIAIRADVPLPLDLLPSFWKILVGEPLEPDVDLQEADVLTYNYVKKFENLNDETELEALCAEIASQHLPMESPDSPNKPSCKFTYVTMTGEEVELCARGRHIPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z100</accession>
    <name>A0A6I9Z100_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2</fullName>
            <ecNumber evidence="18">2.4.3.2</ecNumber>
            <ecNumber evidence="19">2.4.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Gal-NAc6S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Monosialoganglioside sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">ST3Gal II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">ST3GalA.2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Sialyltransferase 4B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36 37 38 39" type="primary">LOC106555616</name>
    </gene>
    <organism evidence="35 37">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37 38" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="36 37 38">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="ChEBI" id="CHEBI:139596"/>
            <dbReference type="EC" id="2.4.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:21617"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a ganglioside GA1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GM1b (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27938"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78568"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:47561"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a ganglioside GA1 + CMP-N-acetyl-beta-neuraminate = a ganglioside GM1b + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:88069"/>
            <dbReference type="ChEBI" id="CHEBI:90151"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a ganglioside GM1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD1a (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:77709"/>
            <dbReference type="ChEBI" id="CHEBI:78445"/>
            <dbReference type="EC" id="2.4.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:18022"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a globoside GalGb4Cer + CMP-N-acetyl-beta-neuraminate = a globoside MSGG + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:140623"/>
            <dbReference type="ChEBI" id="CHEBI:140691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:65373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>ganglioside GM1 (d18:1(4E)/18:0) + CMP-N-acetyl-beta-neuraminate = ganglioside GD1a (18:1(4E)/18:0) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73110"/>
            <dbReference type="ChEBI" id="CHEBI:90153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:48249"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycolipid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="28">Homodimer; disulfide-linked. Homodimer formation occurs in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.2" evidence="18"/>
    <dbReference type="EC" id="2.4.3.4" evidence="19"/>
    <dbReference type="RefSeq" id="XP_013929972.1">
        <property type="nucleotide sequence ID" value="XM_014074497.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013929974.1">
        <property type="nucleotide sequence ID" value="XM_014074499.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013929975.1">
        <property type="nucleotide sequence ID" value="XM_014074500.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013929976.1">
        <property type="nucleotide sequence ID" value="XM_014074501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z100"/>
    <dbReference type="GeneID" id="106555616"/>
    <dbReference type="KEGG" id="tsr:106555616"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047288">
        <property type="term" value="F:beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003836">
        <property type="term" value="F:beta-galactoside (CMP) alpha-2,3-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097503">
        <property type="term" value="P:sialylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23966">
        <property type="entry name" value="GT29_ST3GAL1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000002">
        <property type="entry name" value="CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051757">
        <property type="entry name" value="Beta-gal_alpha2-3_sialyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46032">
        <property type="entry name" value="ALPHA-2,3-SIALYLTRANSFERASE ST3GAL I ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46032:SF1">
        <property type="entry name" value="ST3 BETA-GALACTOSIDE ALPHA-2,3-SIALYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="33">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="34">
        <location>
            <begin position="128"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00042991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00043773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00043816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00062545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00072809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00081228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00081332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00082805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="RefSeq" id="XP_013929972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="RefSeq" id="XP_013929974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="RefSeq" id="XP_013929975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="RefSeq" id="XP_013929976.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="37437" checksum="BC109E91CF5DCF05" modified="2020-10-07" version="1">MMCKKQLWIILALCFALSIWKMYISVSSVSKSLDEWLQQNSLMEEEMCYHNKCIGLSSWDWFCDHFDASVNCLLTLENSDIPPEVLQWWLKLQRSKDGSQIQKMVKYLFDLLQSPTAKTQDIVHCGTCAVVGNSGRLRGSKYGKKIDSHKFVMRMNTAQVTGFEEDVGTRTTHHFMYPESAMNLRPNVHLVLIPFKPLDLKWLASALSTGDIQQTYRKVRRFIKADKSKILIIHPGFLKYIHDKWTRHHGKYPSTGLIALMFAIHTCTRVSAFGFGADIHGNWHHYWEDNLYAGAFRKSGVHNGTVELQLIERLTIEGKLEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z160</accession>
    <name>A0A6I9Z160_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Liprin-beta-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PPFIBP2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the liprin family. Liprin-beta subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929806.1">
        <property type="nucleotide sequence ID" value="XM_014074331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z160"/>
    <dbReference type="GeneID" id="106555474"/>
    <dbReference type="KEGG" id="tsr:106555474"/>
    <dbReference type="CTD" id="8495"/>
    <dbReference type="OrthoDB" id="6516566at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09563">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09566">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09569">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000005">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000007">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000017">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058914">
        <property type="entry name" value="LIPB1/2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037617">
        <property type="entry name" value="LIPB1/2_SAM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037618">
        <property type="entry name" value="LIPB1/2_SAM_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037619">
        <property type="entry name" value="LIPB1/2_SAM_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF18">
        <property type="entry name" value="LIPRIN-BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26022">
        <property type="entry name" value="CC_Liprin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="591"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="668"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="751"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="417"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="483"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="181"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013929806.1"/>
        </source>
    </evidence>
    <sequence length="908" mass="103147" checksum="31AD741622F8F165" modified="2020-10-07" version="1">MTSDASHMLEAALEQMDDIIAGTKAAAEYTNGVYDMVSPAQSVYFGSLQILHLLEDLKLALEMLKDPQEKEALWHQVPGATVACIHDWLEEHLSQVNHHTSTSNETYQERLTRLEGDKESLILQVSVLTDQVEAQGEKIRDLEVCLEGHQLKLNATEEMLQQELLSRTLLETQKLDLMAEVSDLKIKLVGMEKEQSEYEEKQNKAENLLQELKHLKIKVEELENERTQYEWKLKATKAEIAQLQEQLALKDSEIERLQSQISRTSANNEIAEREEVYKRRLKEKYQEVQRLKVGMESLLVANQEKDRRIEELTVLLSQYRKVKEIMLAVQGTSERVLSASGEVDFEATLRKWNHMHAPQIEYKTEIPQGDLAPPILLPASQKILEIRGSSPVPSNKLSLTNGETPEFQNRISQKKISSSLEDLQSESLEKHVDGKPVERDLEEENKSHVKSSKYQTLPGKLPRAAHNEDNHLYNTPATPCCINGNEDNSIQSLRAPDHMDNTTSEELSPLSSGTDSGAQSPLSPDNKRNPKGIKKIWGKIRRTQSGNFPGDDLGLVEFRRGGLRATAGPRLSRSKDTKGPKSDHNAPFAQWDTERVCNWLEEFGLGQYVIFARQWISSGHTLLTATPQDMEKELGIKQPLHRKKLVLAVKAINTKQDDKSAQLDHIWVTRWLDDIGLPQYKDQFHESRVDGRMLQYLTVNDLLFLKVTSQLHHLSIKCAIHVLHLNNFNPHCLRRRPADENTISSSEVVQWSNHRVMEWLRSVDLAEYAPNLRGSGVHGGLIILEPRFNGDTLAMLLNIPPQKTLLRRHLTTNFNVLIGPEAQQDKREIVESTAYAALTTTAKVRPKKLGFSHFGNLRKKKFDESTDYICPMDTSITNGTQRSYGTYRVHNALPDKELDKLDQVGQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z1N1</accession>
    <name>A0A6I9Z1N1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="15">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ATP6V1B2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">Clathrin-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">Secretory vesicle</location>
            <location evidence="12">Synaptic vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930512.1">
        <property type="nucleotide sequence ID" value="XM_014075037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z1N1"/>
    <dbReference type="GeneID" id="106556072"/>
    <dbReference type="KEGG" id="tsr:106556072"/>
    <dbReference type="CTD" id="526"/>
    <dbReference type="OrthoDB" id="1735853at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF5">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B, BRAIN ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="18">
        <location>
            <begin position="40"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="17">
        <location>
            <begin position="163"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="19">
        <location>
            <begin position="395"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013930512.1"/>
        </source>
    </evidence>
    <sequence length="502" mass="55626" checksum="D4F7BD2B50A62E37" modified="2020-10-07" version="1">QRPPDPASRLRRSAPRRRRSPSPGSPASPPSCPVSAYKTVSGVNGPLVILDQVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKRTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDYLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSADNFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTAAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHAEVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVYETLDIGWQLLRIFPKEMLKRIPQSTLAEFYPRDSTAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z337</accession>
    <name>A0A6I9Z337_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Histone acetyltransferase</fullName>
            <ecNumber evidence="17">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">KAT7</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013930391.1">
        <property type="nucleotide sequence ID" value="XM_014074916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z337"/>
    <dbReference type="GeneID" id="106555966"/>
    <dbReference type="KEGG" id="tsr:106555966"/>
    <dbReference type="CTD" id="11143"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036409">
        <property type="term" value="C:histone H3-K14 acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010484">
        <property type="term" value="F:histone H3 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902035">
        <property type="term" value="P:positive regulation of hematopoietic stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045935">
        <property type="term" value="P:positive regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000092">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="18">
        <location>
            <begin position="44"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="16">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013930391.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="38017" checksum="74B3EBA101E59CCF" modified="2020-10-07" version="1">MGSVLYEVSRLMMYNKNNNDNITRTVSIKAVAKEVYKYCQEEHNINGFKNCIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGPISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRCNSNKIMDPSCLKWTPPKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3H6</accession>
    <name>A0A6I9Z3H6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glutamate receptor ionotropic, kainate 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106555984</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930410.1">
        <property type="nucleotide sequence ID" value="XM_014074935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3H6"/>
    <dbReference type="GeneID" id="106555984"/>
    <dbReference type="KEGG" id="tsr:106555984"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06382">
        <property type="entry name" value="PBP1_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000010">
        <property type="entry name" value="Glutamate ionotropic receptor kainate type subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026806803" evidence="5">
        <location>
            <begin position="32"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="6">
        <location>
            <begin position="56"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930410.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="41608" checksum="02935EFC22D50673" modified="2020-10-07" version="1">MKIISPILTHPVFRGTARLLLCLMWIGYSQGTTHVLRFGGIFECVESGPTGAMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPSDTKDAKPLLKEMKRGKEFHVIFDCSHEMAAGILKQGLAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFRILNTENTQVVSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRHKPWRFGTRFMNLIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4A6</accession>
    <name>A0A6I9Z4A6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 12 member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106556222</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013930685.1">
        <property type="nucleotide sequence ID" value="XM_014075210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4A6"/>
    <dbReference type="GeneID" id="106556222"/>
    <dbReference type="KEGG" id="tsr:106556222"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008511">
        <property type="term" value="F:sodium:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055078">
        <property type="term" value="P:sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF93">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="6">
        <location>
            <begin position="11"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="71"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013930685.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="40783" checksum="47CD4B798C0E2DF5" modified="2020-10-07" version="1">MPCFQHYILYLQASGLGRMKPNTLVLGFKKDWRHATAMQVENYTGAIHDAFDFEFGVIIVRTSQGFDISQILQVQGELKKMEQERQALEAALKDNEFEEQNYGLRRFFRRAKKLHFAKTMPPKDCSISTISSVHVGEFNQRMLEASVQFKKKQGKGSIDVWWLFDDGGLILLIPYILTLRKKWKDCKLKIFTGGKVTRLEEDSRQNVVLMASLLSKFRIKFADINIIGDINMKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKREAPWKITDAELEAFKEKSYRQVRLNELLQEHSRAANLIIVSLPVARKGFISDYLYMAWLEILSKNLPPVMLIRGNHKNVLTFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z4D0</accession>
    <name>A0A6I9Z4D0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106557276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013931958.1">
        <property type="nucleotide sequence ID" value="XM_014076483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z4D0"/>
    <dbReference type="GeneID" id="106557276"/>
    <dbReference type="KEGG" id="tsr:106557276"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000002">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000014">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF3">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="43"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013931958.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="26127" checksum="57DFA2933636DC18" modified="2020-10-07" version="1">MVTKEKEQLGLVESSSASPHVTHITIRRSRMLEDGYEQLRQLSQNAMKGVIRVKFVNDLGMDEAGIDQDGVFKEFLEEIIKKVFDPALNLFKTTSGDERLYPSPTAYIHENFLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSIFYSSVDELPSLDSEFYKNLTSIKRYEGDTSDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z541</accession>
    <name>A0A6I9Z541_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small integral membrane protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SMIM14</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930980.1">
        <property type="nucleotide sequence ID" value="XM_014075505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z541"/>
    <dbReference type="GeneID" id="106556515"/>
    <dbReference type="KEGG" id="tsr:106556515"/>
    <dbReference type="CTD" id="201895"/>
    <dbReference type="OrthoDB" id="10054061at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020309">
        <property type="entry name" value="Smim-14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31019">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31019:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11027">
        <property type="entry name" value="DUF2615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013930980.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="11169" checksum="2ABDE4E472620F52" modified="2020-10-07" version="1">MAEGGFDPCECICAQEHAMRRLINLLRQSQSYCTETECLQELPGPNPSAENDMSVTMIMVGWLVIALVLFLMRPISLRRSITSDKPSSPHNGQEPPAPPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z5B8</accession>
    <name>A0A6I9Z5B8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myocyte-specific enhancer factor 2D-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013931055.1">
        <property type="nucleotide sequence ID" value="XM_014075580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z5B8"/>
    <dbReference type="GeneID" id="106556595"/>
    <dbReference type="KEGG" id="tsr:106556595"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013931055.1"/>
        </source>
    </evidence>
    <sequence length="125" mass="12812" checksum="6FF21677B89C8C55" modified="2020-10-07" version="1">MLGGELGNTNGACPSPVGNGYVSARASPNLLPVSNGNSLGKAVPAKSPPPTPQHSNPLATNIRKPDLRVITSQGGKGLLHHLQNAQRLMVSQATHSLTTPVVSVATPSLLTQGLPFSAMPTAYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z7Q8</accession>
    <name>A0A6I9Z7Q8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19 20 21">Toll-like receptor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19 20 21" type="primary">TLR7</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20 21">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endomembrane system</location>
            <topology evidence="15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013932168.1">
        <property type="nucleotide sequence ID" value="XM_014076693.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932169.1">
        <property type="nucleotide sequence ID" value="XM_014076694.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013932170.1">
        <property type="nucleotide sequence ID" value="XM_014076695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z7Q8"/>
    <dbReference type="GeneID" id="106557432"/>
    <dbReference type="KEGG" id="tsr:106557432"/>
    <dbReference type="CTD" id="51284"/>
    <dbReference type="OrthoDB" id="10006997at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032755">
        <property type="term" value="P:positive regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034154">
        <property type="term" value="P:toll-like receptor 7 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000003">
        <property type="entry name" value="Toll-like receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000037">
        <property type="entry name" value="Toll-like receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47410:SF2">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47410">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="14" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="857"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="17">
        <location>
            <begin position="905"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013932168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013932169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_013932170.1"/>
        </source>
    </evidence>
    <sequence length="1065" mass="123477" checksum="A68814F4582FCCA0" modified="2020-10-07" version="1">MLRVPLNVDRMSFAFHQTFHLWLASRLIFFVISLALKEIIVMPFYKTLPCDVNNIIDTSKMYMQVDCSDRKLIQFPKDIPHNVTNLTLAVNRIPKISKSHFINLKKLLEIDFKCNCVPVRLGPKDHVCTQGLKIQAHAFSKLSSLKSLYLDGNQLSAIPQDLPPKLRLLSLEANSIFSIKKGNFSGLKNLEVLNLGQNCYYRNPCNISFEINELAFQPLTSLKVLSLKANNLSKVPQNLPSSLRELYLYNNAIRNITEHDLFSLNNLELLDLSGNCPRCHNARYHCIECPGSILINSKAFDSLKHLKELRLHSTSLQHVDSRWFKNTRNLQVLDLSQNYLARVIQQAHFLQFLPNLVELDLSFNYELDSYPLNFILPKTFSTLSNLQSLRIRGFVFKELNKDTIQNLIPLKKLNVLDFGTNFIRNVNISMFKEFQNLTTIILSFNKISLFSYEPNSRLYSVVNSSDDQYNGNVLKDMQYFRYDEYAQGCRSKNTEDIHSLSFVNETACSNYGKTLDLSRNNIFFIKKSDFQQLSDLKCLNISNNAMSQAFNGTEFAHLSSLKCLDLSNNRIDLLHPNAFQQLKELKVLNLSDNSYYFKAEGVTLMLNFTKCLVNLEILIMNGNEISVSTDNGMESDSIKTLEFRRNRLDILWKDGTNEYLAFFKNLITLENLDISENFLTLLPHDVFYNLPPKLKVLKLASNRLKTFNWGCLPALKNLEVLDLSNNQLTTVPHELSNCTKTLQTFILKNNKIRKLTDNFLKDAFFLRHLDLSFNKIKTIKNSSFPKNVINGLTTLYLNGNFFKCNCDIVWLVHWINETNVKIPFLATDVTCAGPSVWKDKSLILLDMHTCESNYSQILYLLSALFIILLMILPVICHLYVWDVRYIYHFCIAKLKGYKRLFSSDVKYDAFVVYDNKDAAVTEWVLKELIEKLEDQKEKKFNICLEERDWLPGHPLMENLNDSIQMSRKTIFVLTNSYIASGNFRTAFYMAHQRLVDEKVDVIILIILEKLLQMSKCLRLRMTLCRRSCLEWPTNPQSQSYFWQCLRNSLSANNEFRYNKLFKEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z8E9</accession>
    <name>A0A6I9Z8E9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cystine/glutamate transporter-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106557360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013932055.1">
        <property type="nucleotide sequence ID" value="XM_014076580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z8E9"/>
    <dbReference type="GeneID" id="106557360"/>
    <dbReference type="KEGG" id="tsr:106557360"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF246">
        <property type="entry name" value="CYSTINE_GLUTAMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013932055.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="40833" checksum="22E68A89974E53AE" modified="2020-10-07" version="1">MIRPANIAVVSLAFGRYLIEPFFASCHVPPLAVKLVTMAGITLAVVLNCWSVSWAAKVQTGLTVLKMITVGLIIVPGILALRNGHNENFQDSFDTSSLTLEKLPLAFYSGMFAYGGWFYINFVTEEIVNPKKNIPLAAIVSLIVVTICYILTNVSYYAVLTPQEILSSDAVAVSFTDKVFKNISSGILILVALSCFGALNGGIFAASRMLFAAAREGQWPALFSMIHLERHTPVPALILMLPLVYLMVSIGDLYGLLNFYSFSRWFFIGLTTLGFMIHCYRHPTILRPFKVPLFFPLVFTVACFFIVGTSLYSDPVNTSIGCALTLSGLPVYYLVVQKTRIPGCCTSKFNSMTVKLQILMKVTLQEVQMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WY50</accession>
    <name>A0A6I9WY50_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytosolic phospholipase A2 epsilon-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106537463</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907076.1">
        <property type="nucleotide sequence ID" value="XM_014051601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WY50"/>
    <dbReference type="GeneID" id="106537463"/>
    <dbReference type="KEGG" id="tsr:106537463"/>
    <dbReference type="OrthoDB" id="419768at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF24">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2 3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="4">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013907076.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="43085" checksum="B6B7CD2D090D2126" modified="2020-10-07" version="1">MTTGGGTRSFTAMYGSLLGLQKLNLLDCITYLTGLSGTTWTMCKLYEDPDWSQKYLEEAINKARRQVIKSKFNCFSPDKLKYYYDELSQKIKEGHSTSFIDLWGLIIESMFHDEKDNHTLSDQRMALNEGQNPFPIYLAVNLKDNYSAQDFKEWLEFTPYEVGSMKYGAYIRSEDFGNEFFMGRLMKKFPEIRICFMQGMWSSIFSLDLIYFWNVANDSEEFWYMWTRDRIQDIGKNLDQFSKYASELGVPFPKTEISEEDKNNLKECYVFEDTENTAAPLVLFFPLTNDTFKAFEAPGVERLPTNMEEGNVDVSSFFSPFGTREVSFSEDNFDKLVKLAEYNILNNEDKIIEAFHAAVQRKKYQKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9WZC4</accession>
    <name>A0A6I9WZC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Intestine-specific homeobox</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">ISX</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transcription factor that regulates gene expression in intestine. May participate in vitamin A metabolism most likely by regulating BCO1 expression in the intestine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013908318.1">
        <property type="nucleotide sequence ID" value="XM_014052843.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013908319.1">
        <property type="nucleotide sequence ID" value="XM_014052844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9WZC4"/>
    <dbReference type="GeneID" id="106538377"/>
    <dbReference type="KEGG" id="tsr:106538377"/>
    <dbReference type="CTD" id="91464"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000369">
        <property type="entry name" value="Intestine specific homeobox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF362">
        <property type="entry name" value="INTESTINE-SPECIFIC HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="3 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="62"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="64"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013908318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013908319.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="19813" checksum="B41383765887C7BF" modified="2020-10-07" version="1">MSCSKECDKKEKPILSFSIEAILKPPSTEACLHNTVTCNSQTEVAQKFNSEPLLDRYKDDKKMKRRIRTTFTAEQLKELERIFQATHYPDINIRNQLAAKINLPETRIQIWFQNQRAKWRKHERFSNFAGLQPQTKAGFISVPKPKDLFMMPSNSLSGIIPPSEYYYTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X0N3</accession>
    <name>A0A6I9X0N3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Atypical kinase COQ8A, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Chaperone activity of bc1 complex-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Coenzyme Q protein 8A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">aarF domain-containing protein kinase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ADCK3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is still unclear: may act as a protein kinase that mediates phosphorylation of COQ3. According to other reports, acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates. However, the small molecule kinase activity was not confirmed by another publication. Shows an unusual selectivity for binding ADP over ATP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908799.1">
        <property type="nucleotide sequence ID" value="XM_014053324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X0N3"/>
    <dbReference type="GeneID" id="106538733"/>
    <dbReference type="KEGG" id="tsr:106538733"/>
    <dbReference type="CTD" id="56997"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF1">
        <property type="entry name" value="ATYPICAL KINASE COQ8A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="20">
        <location>
            <begin position="337"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_013908799.1"/>
        </source>
    </evidence>
    <sequence length="685" mass="76192" checksum="003E40F7FC7C56E3" modified="2020-10-07" version="1">MYLATEHEAPFLLLVPCSCGYSLSSKLTQIVLEVIVMAGDAIMMVRGLTKLSKAVLETQAGQLRQVILGGDALTIARSFQAVAEEQFSAAMGKMQELGKQQENLKDIGEDFGKEYNFSESELGGITKDFSSAQDQPHKHSGTEGPSYSYDIKGPVQSGGSGDATASQNPISGKINTKLFEGFRNPGNPFALVFGQIRPFHQDHSPVGGLTAEDIDKARQAKAGPQQKPHKQMLSERARERKVPVTRIGRLANFGGLAVGLGIGALAEVAKKSLRSEERNGKKAILDSSPFLSEANAERIVSTLCKVRGAALKLGQMLSIQADDAFINPQLQKIFERVRQSADFMPLKQMIKTLNNDLGPNWRDKLESFEERPFAAASIGQVHLARLKNGKEVAMKIQYPGVAHSINSDVSNLMTVLSMSNVLPEGLFPEHLIEVIRRELALECDYKREAACAKTFKELLKDHPFFYVPAVVDELCGQSVLTTELVPGFPLDQAEDLSQEIRNEICHNILVLCLRELFEFRYMQTDPNWSNFFYDPESHKVALLDFGATRGFDEKFTDTYIELIKAAADMDSERVLKKSVEMKFLTGYENKAMEDAHLNAVLILGEAFASHEPFDFGCQKTTEKIHGLIPVMLKHRLVPPPEETYSLHRKMGGSFLICSKLKAKIPCKNMFQEAYSKYWSKKTKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X193</accession>
    <name>A0A6I9X193_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein max</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myc-associated factor X</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MAX</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013909019.1">
        <property type="nucleotide sequence ID" value="XM_014053544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X193"/>
    <dbReference type="GeneID" id="106538905"/>
    <dbReference type="KEGG" id="tsr:106538905"/>
    <dbReference type="CTD" id="4149"/>
    <dbReference type="OrthoDB" id="8964853at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11406">
        <property type="entry name" value="bHLHzip_Max"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000023">
        <property type="entry name" value="MAX isoform 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328:SF3">
        <property type="entry name" value="PROTEIN MAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328">
        <property type="entry name" value="PROTEIN MAX MYC-ASSOCIATED FACTOR X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="14"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="124"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909019.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="20012" checksum="CAF2892F1129D48C" modified="2020-10-07" version="1">MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKMNASCYMLNTGILQDTAERFMAIWASRAQILDKATEYIQYMRRKNHTHQQDIDDLRRQNALLEQQVRALEKARSSAQLQANYSSSDNSLYTNSKGDTISAFDGGSDSSSESELEEPQSRKKLRMEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X5Y4</accession>
    <name>A0A6I9X5Y4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bcl2-associated agonist of cell death</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BAD</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910854.1">
        <property type="nucleotide sequence ID" value="XM_014055379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X5Y4"/>
    <dbReference type="GeneID" id="106540304"/>
    <dbReference type="KEGG" id="tsr:106540304"/>
    <dbReference type="CTD" id="572"/>
    <dbReference type="OrthoDB" id="8991151at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018868">
        <property type="entry name" value="BAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28540">
        <property type="entry name" value="BCL2-ASSOCIATED AGONIST OF CELL DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28540:SF1">
        <property type="entry name" value="BCL2-ASSOCIATED AGONIST OF CELL DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10514">
        <property type="entry name" value="Bcl-2_BAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910854.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15341" checksum="F06D0B62F14B35F3" modified="2020-10-07" version="1">MFHISEFQDETFSTNEKDPTDLKPSVGGAKDLNSPSHENPELRRRIGSDQLDSETSDEVGTFRARSRSAPPILWAAQRYGRELRRMSDEFHGALQKLPRPKSAGTCSRMDHPPSWKDTLRSWWRRSCPENGPSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X6K4</accession>
    <name>A0A6I9X6K4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106539244</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106539244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013909472.1">
        <property type="nucleotide sequence ID" value="XM_014053997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X6K4"/>
    <dbReference type="GeneID" id="106539244"/>
    <dbReference type="KEGG" id="tsr:106539244"/>
    <dbReference type="OrthoDB" id="1884872at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013909472.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="50120" checksum="8F089F08E8105EA0" modified="2020-10-07" version="1">MESTTYHSEKAGTKKENSRKQIGLTSEILSTSCRSRNKSLKQNTIIKASFVLLKNINRGRKEKHDYYLTAPRGKQLAPPTQHKKFPAFRKLQVALYDILDKNCVATLKKSFQISNVGALVEKEKKDTTERGTTEVDNSMEVGEFSGELDPGQTNSDCDLRKSISRMILTDKKSHEGDRCQVSERLSSFSEINLKLDLQTLMLNKSEIKLPIKEMMGLSLNTLTEKVREDQTTLEEEESNTRNSSKNTERFWFLLKNNEIRYDDAPVCVADEKMAGDLVCRDDGLSDHSAECLLSDGNLANQTYPDENDGAFIHTEIKKATMSVEDQEVSFFNDEKENMDCLPAEENISVEEEENFPLCQSQSVKMVFYELFGSLVKLLGSPSVNQKKSRLGRLTELEDSSDAEKREGSDTKSSSKRKKEDRLCLANRAMHLDCRTKEKTTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X8N1</accession>
    <name>A0A6I9X8N1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zona pellucida sperm-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106537901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013907658.1">
        <property type="nucleotide sequence ID" value="XM_014052183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X8N1"/>
    <dbReference type="GeneID" id="106537901"/>
    <dbReference type="KEGG" id="tsr:106537901"/>
    <dbReference type="OrthoDB" id="9903747at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060468">
        <property type="term" value="P:prevention of polyspermy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057638">
        <property type="entry name" value="Ig_ZP2_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057636">
        <property type="entry name" value="Ig_ZP2_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF4">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23736">
        <property type="entry name" value="Ig_ZP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23740">
        <property type="entry name" value="Ig_ZP2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zona pellucida sperm-binding protein 2 second Ig-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Zona pellucida sperm-binding protein 2 third Ig-like" evidence="2">
        <location>
            <begin position="197"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013907658.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="26438" checksum="D8507840E54593A2" modified="2020-10-07" version="1">MEDCRYQIDYGKQVLMVGHQNCTEMEDNKPRLRFRLQFNDTEGVRPQNETYSVDCDAPQADEARSNSGFASATTNCTKTNMAVTFPRLIPSFRDELTGVRPGMGSQVAPGMEWMVSIEDNGQIHHLLLRQAIQQGYTFFSDATDIIIQVPFNARGVAVYKQDDHVLYTVAIKLTYGPPEQRLTLESRMICAPGPAICNSTHMMVILPAFPGKLTAVSLNEKNIPVHQLQANDIGVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9X907</accession>
    <name>A0A6I9X907_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Bombesin receptor-activated protein C6orf89 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106541212</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912064.1">
        <property type="nucleotide sequence ID" value="XM_014056589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9X907"/>
    <dbReference type="GeneID" id="106541212"/>
    <dbReference type="KEGG" id="tsr:106541212"/>
    <dbReference type="OrthoDB" id="10036464at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038757">
        <property type="entry name" value="BRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35259">
        <property type="entry name" value="BOMBESIN RECEPTOR-ACTIVATED PROTEIN C6ORF89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35259:SF1">
        <property type="entry name" value="BOMBESIN RECEPTOR-ACTIVATED PROTEIN C6ORF89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013912064.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="31482" checksum="DEE1E1B1D85DB152" modified="2020-10-07" version="1">MELSTSELSIYDKLSETIDLVRQTGHQCGMSEKAIEKFIKQLLEKNEPQRGPPRFPLLMVLYKGLVTLGVVLLTAYFMIQPYTLSPSENTLTKNHPLGSLFHHIRLLPLPITKKYMLEKCPEWWSLDCRWNMSLTSKCSCCCSMRTLVAPVDIRQLSDVRQLQGQPLLIKTGQYRSYAEMKHFQSLYPQLTNFVIQEESAELWSSPSNKQQSPLPVFWQIPQNKSQILQEIFPIFSSLLFPKDISLKSCFLIHHPRLQDKTYRLQNTFVVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XAI9</accession>
    <name>A0A6I9XAI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">YLP motif-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear protein ZAP3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">YLPM1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in the reduction of telomerase activity during differentiation of embryonic stem cells by binding to the core promoter of TERT and controlling its down-regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PPP1CA and NCOA5. Forms a complex with ILF2, ILF3, KHDRBS1, RBMX, NCOA5 and PPP1CA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013912614.1">
        <property type="nucleotide sequence ID" value="XM_014057139.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106541638"/>
    <dbReference type="KEGG" id="tsr:106541638"/>
    <dbReference type="CTD" id="56252"/>
    <dbReference type="OrthoDB" id="513595at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032204">
        <property type="term" value="P:regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000399">
        <property type="entry name" value="YLP motif containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058903">
        <property type="entry name" value="Spectrin_YLPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026314">
        <property type="entry name" value="YLP_motif_con_p1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413">
        <property type="entry name" value="YLP MOTIF CONTAINING PROTEIN NUCLEAR PROTEIN ZAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413:SF0">
        <property type="entry name" value="YLP MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26583">
        <property type="entry name" value="Spectrin_YLPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="YLPM1-like spectrin repeat" evidence="14">
        <location>
            <begin position="111"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="237"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="461"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="562"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="606"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="670"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="913"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1379"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1446"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1568"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1633"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2013"/>
            <end position="2040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="690"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="705"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="732"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="943"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="966"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1071"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1096"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1481"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1644"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1661"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1700"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1780"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1793"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1806"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2018"/>
            <end position="2028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF26583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013912614.1"/>
        </source>
    </evidence>
    <sequence length="2144" mass="242371" checksum="9F91642C7F14B74B" modified="2020-10-07" version="1">MTSQEQQQYWYQQHLLTLQQRAKAHALGQQQPPIGNDAPEQGKEKRETSVSSVLSEPSLNFDPMLQHPKDEEPPESSVKHKLNIEPSEDPEESLRLQQLQAAATQWQQHPQQRVGFQYQRIMQKHSQLQQILQRYQQIIQQPSHLSTATMEVQLQHYKTQNKQFQPLYKEWEREFQQWQDQLLTYPHKDQLYEYEKQWKMWQGQMKATYAHLQEKVKSLQNLNTPCPGSTFPFSPYSQTVQSSMPASTLPPSTPGFSTGPAESGPKSTQTTSNSFSFKVNESLSSSQVVGEIRPGLLPTPFSCSFTSKTENSSTTSQSIEMRPTLFPSPTSFSFKVTESAPTSQNTETARLALLPTPNPTETTRPALLPTPAAAFSFKVNDSNAATQVTETTRPALLPTPSPASFSYTDTSATLPYHSNVTASAISSDQGDPYAPLGKQGVALGSASSDHVKVNLESTIASSSMSTTTVTTTTDYQNRALRPAGFPDSPRAPFYEGPKGPRFDGPQGRGFEPPKQGGPNFQGQHLEDPGARLQNQHADGSSGKQRSIPRGPAAAFYTPLATSQMPSSQASGPLVRGSKPASGQQNHQGENKELLIDTVNKSKFMSDVNVSGPVQPAKGLPNVQQNTSRKNAIEAMSEIKKQSAMDSRLHSEASKSYCTSTSLHQIPVSNISQTTGGKKTVLPSPIIGNKDMNSTQNKLVTSGNNKNKESSKLENREKGEKKPGDNQGIGPADRGRGQYRMEDTRGRGRGRLEDMRGRGKANRGRDHSEDVVGRDKSSGLGTHDKIPGGQLSNWESGMGRQGGNQERVLGKQSNIQERGLPAPQMGSRERIQANSQQRGLPAPRISRWGPIPANIQERGLPAPQMGSRETIPANSQERGLPVSQMGSREIIPPNNQEKGLPAPQIGSKEIIQANSQQRGLPAPRISRWGPIPANNQERGLPASQMGSRETIPANSQERGLPASQMGSRERIQANSQERRLSGPRISRWEQIPANIQERGLPAPQMGTRERIQANNQERGLPAPQMGSRERIQANIQERGLPAPQMGIRERIQANSQERGLPAPQMNSRERIQANSQESAVIRSSSSQERGLDWQIGSRERAPGRQPTNRERETDRQGNDLEREPIRRPSNREQDLHRRDMGLGGNAENLPPFYEEPPSPPWSYEEQSRKEGISFDYRHIPPLEHRQLDDWERERYWHDQDQRYDDSIVVYEIDEGVPQHHPLPPLSPLPPLPPLPPLVDEEPFWEIPLSRDGEGDRDLERIRRALDGYDCDFEIDWDRDYIILSGDRDTLNCDMDIPPLPPLPPLPPLPPIDRYHEDVWLEERDIDFYDRDSRDRSGIRIREYPERCDTWREQSDFPPDRGDWERERFSERWYPDEMDRFKSLDDRLDPPPMPPRSSELLEEESNLASEQSLGGLVLSQRQHEIILKAAQELKMIREQKEHMQKLNEFGPDAQMSERSSQEISRLQRIPARKDNFQASSETQGKRTAEKPAIKPTPAVIIKPPVMFHKPAATTKPCPVKSSPVTKPSVSAAKVPIPTAKPSGLPLQSNVPVSIPTVAATQVSVPVVKTSTETMEERWDEDTFQGLWDSNEDKGLKSECVSSISNSALPSTTISASPLPNVAPLAKAPIIQQTVDYGHGHGHSHGQDITNSKSAQQVPYGERITLHPEPHERQPFSKEHLGQRDRYGKERESYFERQNNSNPDHRDFRREREPYRERISGDHDRERFDKERYSREDRPSPSRNQSYRDKDHSVSRRGGFERQTYDRKPDRPSYDHGPPMFGGDRRNYAEDRIPISAPPIPRQPPPAPRVERKPESKNVDDILKQPGRESRPERIVIIMRGLPGSGKTHVAKLIRDKEVECGGAAPRVLSLDDYFITEIEKEERDPDTKKRVKKKVLEYEYEADMEEIYRASLFKTFKKTLDDGFFPFIILDAINDRVRHFDQFWSAAKTKGFEVYLAEITADHQTCCKRNVHGRKLKEINKMADHWETAPRHMIRLDIRSLLQDAAIEEVEMEDFDANIEDQKDENKKDAEEEESELGYIPKSKWEMDTSEAKLDKLDGLRTGTKRKRDWEAIASRMEDYLQLPDDYDSRASEPGKKRVRWADLEEKKDADRKRAIGFVVGQTDWEKITDESGRFAEKALNRTKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XBX3</accession>
    <name>A0A6I9XBX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of G-protein signaling 21-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106542006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013913079.1">
        <property type="nucleotide sequence ID" value="XM_014057604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XBX3"/>
    <dbReference type="GeneID" id="106542006"/>
    <dbReference type="KEGG" id="tsr:106542006"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF160">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="1">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013913079.1"/>
        </source>
    </evidence>
    <sequence length="145" mass="17126" checksum="451D382D7ED3AA20" modified="2020-10-07" version="1">MKHSQGLKSDVIYKQSIFKKLRDGLDAFRAFLKSEFSEENIEFWLACEDFRKTKLAATIASKAQRIYSEFIQADAPREINIDFNTRDRITQAISKPTLSCFDDAQQLIYTLMAKDSFPRFLKSEAYKELVNKELKRRQKRWLPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XC85</accession>
    <name>A0A6I9XC85_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Synphilin-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SNCAIP</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013911462.1">
        <property type="nucleotide sequence ID" value="XM_014055987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XC85"/>
    <dbReference type="GeneID" id="106540747"/>
    <dbReference type="KEGG" id="tsr:106540747"/>
    <dbReference type="CTD" id="9627"/>
    <dbReference type="OrthoDB" id="10057496at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040133">
        <property type="entry name" value="SNCAIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032027">
        <property type="entry name" value="SNCAIP_SNCA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22882">
        <property type="entry name" value="SYNPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22882:SF3">
        <property type="entry name" value="SYNPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16700">
        <property type="entry name" value="SNCAIP_SNCA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="448"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Synphilin-1 alpha-Synuclein-binding" evidence="4">
        <location>
            <begin position="504"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="35"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="541"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="664"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="724"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="813"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="870"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="548"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="665"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="677"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="749"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="870"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="889"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013911462.1"/>
        </source>
    </evidence>
    <sequence length="954" mass="105962" checksum="6AC5314D7E5E58C1" modified="2020-10-07" version="1">MEPPEYLDLDEIDFSDDISYSVTSFKTIPELRRKCDSQNEDRTVSTTNWNSGVSTLLGNTQKPTGIADMYSKFRPVKRVSPLKHQPESSENNESDDQKNQKVDYKKGDDSPSMTQNDDQTPDNGDSLKTKCVEPGVLLGELEHYDLDMDEILDVPYIKSNQQLATFSKAAPEKRILNVYSTLNGLSSKSGPVGDTEKAPTGTTQFCVLSPVKGSQLKKAHSIVTDQHKQLVEEHEFPQPLIKSGSVHEPEAHSKGFLNRTSAEKMEKSVSHCQLRTFHLQSTESKHDKLSSSVNWNSSGNSEEKNEDVKKTKSILNIVKEGQISLLPHLAADNLDKIHDESGNNLLHVAASRGHAECLQHLTSLMGEDCLNERNSEQLTPAGLAIKNGQLECVRWMVSETEAIAELSCSKDHPSLIHYAGCYGQEKILLWLLQFMQEQGISLDEIDQDGNSAVHVAAQHGYLGCIQTLVEYGANVTMQNHVGEKPSQSAERQGYTMCSRYLVVVETCMSLASQVVKLTKQLKEQTVERVGLQNQLQELLATQRADGKSQSTSSSSPSSPISGKPQWKQSDTDDVTQSKSKLNTHDGIQIHGSAGTHHPTRPKLKEEESDKILRQLLGKEISENVCMQEKLSLEFQDVQASKNTKKVPLEKKELKLARLKHLMQRSLSESDTDSNNSEDQKNTPVKRIDKPRPRPIVENTESTENLHLMIKKHALASGRRFPFGIKTSKSVDGHSPSPTSESSDPDNESHYQTGTVSQSQVGGDPNHANSDTTIAPKIATSPKSALKSPSSKRRTAQNSKLRVTFEEPVVQLEQTEIGLNGEKDKEQNKSLPRPIPNADTGDQQKRPFGTFRSIMETLSGNQNNNNNYHSSSLIKTSSPFTSLGRKATDSKGNSATSTKGKNKPALCSSYISFSTRMQIEDHLDNNAWHNSYNEKPQSFYSKNCFEEPELQELFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCC2</accession>
    <name>A0A6I9XCC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Focadhesin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FOCAD</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013913234.1">
        <property type="nucleotide sequence ID" value="XM_014057759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542111"/>
    <dbReference type="KEGG" id="tsr:106542111"/>
    <dbReference type="CTD" id="54914"/>
    <dbReference type="OrthoDB" id="6125419at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060147">
        <property type="term" value="P:regulation of post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022542">
        <property type="entry name" value="FOCAD/RST1_DUF3730"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045163">
        <property type="entry name" value="Focadhesin/RST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021392">
        <property type="entry name" value="Focadhesin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16212:SF4">
        <property type="entry name" value="FOCADHESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16212">
        <property type="entry name" value="FOCADHESIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12530">
        <property type="entry name" value="DUF3730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11229">
        <property type="entry name" value="Focadhesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3730" evidence="2">
        <location>
            <begin position="489"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Focadhesin C-terminal" evidence="1">
        <location>
            <begin position="916"/>
            <end position="1503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013913234.1"/>
        </source>
    </evidence>
    <sequence length="1503" mass="168850" checksum="7E771006283AED6E" modified="2020-10-07" version="1">MSEEIRKRLDFPNTLIQSQAVGHLIAAVLKENVLSNKISQSSNQTPALNLLWEKSCSDNMMVRTTCCEAVVKLVKQDHAEFGYILNTALSLIPSARNVQGLIKIIVKLLQMQALKTSQSEEQQQLDLYAIRNSPHPLIIIVENRPDCWPFLLQHMTTFFQQCPKGAERACVNIMRPFLRYLYCEPYQLREHAELRMSLLRVLLQPYGPFNKEEPPGLECHLLQLFCDLVPYYQVKDALQITEALLFLEGLFRSLLRYPSFWKKQLCHLCLQLLVFCEVSLQATGECSSLIRLMEENVELLKEGFPFQMTIIGLGVLLLQIPASQQKPILNLAIKLLSGSETKMTPLMAVILVMPALQILSSTTGQDNMIEENDSTTRQQLAINLLEIVQQEELKEQPLVSWEHYFPINSTYGSLHVTWQLLWLLRDKTAMLDWLSSIKSMLPIATQVSVHVVLLVAYLLIQENGDNLHSVLDATIEIAKVDSSQVPNLIPVLMFKLGRPLEATLCRDILYTLPALGVHKVCVAQILRVLQVLGNTPKLQAVTLRLMTCLWEKQDRVYPELQRFMAMSDMASLSVSKDTQWEKVIAKAASIRDICKQRPYQHGADMLAAISKVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLNCDTRPFILKTLNELFSLVPSLMVNSSEYEKFKAQVVSFLWSHTQSKDSLVAISAYKSLSEFHPEEYTILHLPEQKSYSGENTASAIARSCAATSLSLLVPVFIVSYKERVEEVLNLLIAMLPGKPNADESQAVQIHMSLALGMFISRLCEEKVSDVADKQMNLLLMKSLEALEDCCFDTSLEYNTGCILGVGLVLSLMSHSNLTDSEAHISTSLRKLCKYLDDAEDQSRTLQEILAYTIACVCVSAFSTRIVEATEAEDVMDKLQKLTEMNQQTPGFALALGSLVHGLSICGHGKADVLSNKLFPAWMKTVLAEGAPTMQRLAAVNGLVSLVGSESTMIQLKSEAIQSSHFQSKLNEVIRTLTQVISFSGVIGLQVNAAWMLGHLYLSNVSATRSRTSVPSDFSYLPEKSFLRATIDFIIEGGKKGPEQVHPSFLKAAMAPIALIGGSYQYPPLNWASILAPLLRLDFGEEIQQLCIELAVTQAQSSQNAAMILGMWVAPPLVYSLSIQAKRYLFASLPLWMKYVAEDKQQIFTEVFMVQHFETKKQSKNPDLCWNILQGLSQAMKLPSPTQHSWSCFCKAAEKIFELLPDEIWQNDVKMYILAAKCLSEMVDTEVERITEVSKNNLEKVAFVRVYLVSQGRFPLLRWNDVISVAAGCQQKETIVWMLLHSFYHARILSHENTGVLKRMEWLLEFMGYIKKVSLNTASMQNVSPQEAVSFLLWIFAACVVAWADHALPMLLGLSADWSAWQCETIDRVLARGLGKRPVDTLAVKEILTLLPGSLQILLTKEPWKEQTPKFIDWLFSLMENANEILTQSSRDLLKASLLALRSLPEFKKKAVWTKAYGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XCU0</accession>
    <name>A0A6I9XCU0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Unconventional myosin-VIIb-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106540897</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911667.1">
        <property type="nucleotide sequence ID" value="XM_014056192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XCU0"/>
    <dbReference type="GeneID" id="106540897"/>
    <dbReference type="KEGG" id="tsr:106540897"/>
    <dbReference type="OrthoDB" id="10055605at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17093">
        <property type="entry name" value="FERM2_F1_Myosin-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13199">
        <property type="entry name" value="FERM_C2_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000012">
        <property type="entry name" value="Myosin VIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041794">
        <property type="entry name" value="MyoVII_FERM_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF24">
        <property type="entry name" value="MYOSIN VIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="11">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="10">
        <location>
            <begin position="155"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013911667.1"/>
        </source>
    </evidence>
    <sequence length="468" mass="54743" checksum="C52EE037F65051AA" modified="2020-10-07" version="1">MLHLEEVLSWFVVCTYFFVKSFLIFFCSPIMKYMGDYPSKQERSPVELTDQIFLVAIQEDTLQDEVYCQILKQLTENHNRYSLNNGWQLLWLCTGLFPPSKSLLKHVQKFIETRLKENLALECRHRIQKVMRSGCRKWAPHSVEVEAIQNNITKISHKIHFPNETEQVFDVGTNTKVWDLCQMIGNKMELSSLDGFSLFIKISDKVISQNEADYFFDSLRQVTDWTRKNRPGKEVFPTTMNYQVYFMRKLWLNVIPGKDLKADCIFHYHQELPKYLRGYHKCSKEDVVQLAGLIFRVRFNSDRTQYAVIPKILKELVPENMIPILSPEEWKRNIISAVSKLGQKSVDEAKIAFLKFIYRWPTFGSAFFEVKQTSEPNFPDIVLIAINKQGISIIHSKTKEILALYPFNKISNWSSGSTYFHMTIGNLVRGNRILCETFLGYKMDDLLTSYVHLLMNIVNKQKRPNIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XDC2</accession>
    <name>A0A6I9XDC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Methionine synthase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MTR</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the vitamin-B12 dependent methionine synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913624.1">
        <property type="nucleotide sequence ID" value="XM_014058149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XDC2"/>
    <dbReference type="GeneID" id="106542423"/>
    <dbReference type="KEGG" id="tsr:106542423"/>
    <dbReference type="CTD" id="4548"/>
    <dbReference type="OrthoDB" id="261426at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046653">
        <property type="term" value="P:tetrahydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02069">
        <property type="entry name" value="methionine_synthase_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1240.10:FF:000001">
        <property type="entry name" value="Methionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.280:FF:000001">
        <property type="entry name" value="Methionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.288.10">
        <property type="entry name" value="Cobalamin-dependent Methionine Synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="entry name" value="Methionine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.196.10">
        <property type="entry name" value="Vitamin B12-dependent methionine synthase, activation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050554">
        <property type="entry name" value="Met_Synthase/Corrinoid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033706">
        <property type="entry name" value="Met_synthase_B12-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004223">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037010">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45833">
        <property type="entry name" value="METHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45833:SF1">
        <property type="entry name" value="METHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02965">
        <property type="entry name" value="Met_synt_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01018">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="Cobalamin (vitamin B12)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56507">
        <property type="entry name" value="Methionine synthase activation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47644">
        <property type="entry name" value="Methionine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50974">
        <property type="entry name" value="ADOMET_ACTIVATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51337">
        <property type="entry name" value="B12_BINDING_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="B12-binding N-terminal" evidence="10">
        <location>
            <begin position="76"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="9">
        <location>
            <begin position="186"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="AdoMet activation" evidence="8">
        <location>
            <begin position="337"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013913624.1"/>
        </source>
    </evidence>
    <sequence length="741" mass="84561" checksum="E29D91B4FB7462DF" modified="2020-10-07" version="1">MEAIREAMHGVFLFHAIKHGMDMGIVNAGNLPVYDDIQKELLQLCEDLIWNKDPDATEKLLHYAQTHAQGGKKVVQTDEWRNGAVEERLEYGLVKGIEKYVIEDTEEARQNKERYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKDREELRALSGSTEEDNPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALENKADIIGLSGLITPSLDEMISVAKEMERLEIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDETLKDDFFEEIRDEYEDIRQDHYDSLKERKYLSLEQARNKRFYCDWKTETKPVKPQFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGRYPNRGFPKIFNDKVVGEEAKKVYNEAQNLLKSLITENKLKATGLIGFWSARSIKDDIYLYDTEEKLHNLEPIARFCGLRQQVEKDSGSTDPYYCLSDFISPLESDVPDYLGIFAVACFGVDELCKEYEKQSDDYSIIMVKALADRLAEAFAEELHERVRKEFWAYCTNEQLEFSDLRKIRYDGIRPAPGYPCQPDHTEKITMWRLANIEEATGIQLTESLAMFPASAICGLYFSNPASRYFAVGKILKDQVRPFCCYRNCIKKERAVNGNYIQKHTIFRPRVPNPSPPPNASTQNCICTSSVHVKPSSLQHHCFRHLQSPEPERLGTADLDYSIWYLSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XFS3</accession>
    <name>A0A6I9XFS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NDUFAB1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913016.1">
        <property type="nucleotide sequence ID" value="XM_014057541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XFS3"/>
    <dbReference type="GeneID" id="106541955"/>
    <dbReference type="KEGG" id="tsr:106541955"/>
    <dbReference type="CTD" id="4706"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000008">
        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 14" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="15">
        <location>
            <begin position="68"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013913016.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16240" checksum="CCED4A8850151E8F" modified="2020-10-07" version="1">MAARVLLRAVARVRVQRRPLVPRLGASGTQGVGRGLAGAARAGLQLPAPAQRPAWVRCYSDAPTLTLKSIHDQVLYVLKLYDKIDPEKLTAESHFMKDLGLDSLDQVEIIMAMEDEFGFEIPDAAAEKLMSPQDIMDYIADTQDVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XG20</accession>
    <name>A0A6I9XG20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carnosine N-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CARNMT1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">N-methyltransferase that catalyzes the formation of anserine (beta-alanyl-N(Pi)-methyl-L-histidine) from carnosine. Anserine, a methylated derivative of carnosine (beta-alanyl-L-histidine), is an abundant constituent of vertebrate skeletal muscles. Also methylates other L-histidine-containing di- and tripeptides such as Gly-Gly-His, Gly-His and homocarnosine (GABA-His).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carnosine N-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013909998.1">
        <property type="nucleotide sequence ID" value="XM_014054523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XG20"/>
    <dbReference type="GeneID" id="106539677"/>
    <dbReference type="KEGG" id="tsr:106539677"/>
    <dbReference type="CTD" id="138199"/>
    <dbReference type="OrthoDB" id="978at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030735">
        <property type="term" value="F:carnosine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035498">
        <property type="term" value="P:carnosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000094">
        <property type="entry name" value="Carnosine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012901">
        <property type="entry name" value="CARME"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303:SF6">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07942">
        <property type="entry name" value="CARME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01296">
        <property type="entry name" value="N2227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013909998.1"/>
        </source>
    </evidence>
    <sequence length="457" mass="53006" checksum="C159F53FAD9AC02A" modified="2020-10-07" version="1">MNPEARQHARKRARGARAAVRFRRRRKAAEMSVSTACALSRPGRQEPPPPRVKAMRGDSRESEEDEEQAEPPRRDGEAAAENTLCERDSLPQLATTKLDGKEEEKQEREHFRRIINAFRYYGINMHERVTRAERQFRTLPAIQQKLLPQFLPHLDKIRKCVDHNQGILQSVVNDCTRMFENREYDGDGNGKITPASTFDMDKLKSTLKQFVRDWSETGKPERDSCYQPIINEIVKNFPKDRWDLSRINVLVPGAGLGRLAWEIAMLGYTCQGNEWSLFMLFSSNFVLNRCCETNSCKLYPWIHQFSNNKRSADQIQPIYFPDVDPHSLPPGANFSMTAGDFREIYSESNIWDCIATCFFIDTAHNVIDYIDTIWKILKPGGIWINLGPLLYHFENLANELSIELSYEDIKNVILQYGFHIEMENESVLTTYTVNELSMMKYYYECVMFVVRKPESSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGA0</accession>
    <name>A0A6I9XGA0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ankyrin repeat domain-containing protein 29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ANKRD29</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013910063.1">
        <property type="nucleotide sequence ID" value="XM_014054588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XGA0"/>
    <dbReference type="GeneID" id="106539722"/>
    <dbReference type="KEGG" id="tsr:106539722"/>
    <dbReference type="CTD" id="147463"/>
    <dbReference type="OrthoDB" id="7464126at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44207:SF2">
        <property type="entry name" value="REPEAT PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44207">
        <property type="entry name" value="SURFACE ANTIGEN BSPA-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013910063.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="32205" checksum="463EA6CF0B0DDDD2" modified="2020-10-07" version="1">MSFKKETPLANAAFWAARKGNLALLQLLLNSGRVDVDCKDSLGSTALMVASYYGHKECVRELVLQGADINLQRETGATALFFAAQQGHNDIVKLLFEYGASTEFKTKDGGTALLAACQYGHMRVVDTLLKHGANIHDELYDGATSLFLAAQGGYLDLIRLLLSSGAKVNQPRQDGTAPLWIASQMGHSDVVRVMLLRGADRDAARLDGTTALLKAATKGYNEVIEELLKFSPALGILKNGTSALHEAVLGGNIRAVALLLEAGADPILKNKKNELPSDLTKNDRILRLLQSRAKHRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XGM8</accession>
    <name>A0A6I9XGM8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1</fullName>
            <ecNumber evidence="10">2.1.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">TAR RNA-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TARBP1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in a subset of tRNA. Selectively mediates Gm18 methylation of tRNAGln-TTG/CTG and tRNASer-TGA/GCT. Gm18 modification can enhance the stability of modified tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O-methylguanosine(18) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20077"/>
            <dbReference type="Rhea" id="RHEA-COMP:10190"/>
            <dbReference type="Rhea" id="RHEA-COMP:10192"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="EC" id="2.1.1.34"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:20078"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer and homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.34" evidence="10"/>
    <dbReference type="RefSeq" id="XP_013914844.1">
        <property type="nucleotide sequence ID" value="XM_014059369.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543364"/>
    <dbReference type="KEGG" id="tsr:106543364"/>
    <dbReference type="CTD" id="6894"/>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141100">
        <property type="term" value="F:tRNA (guanine(18)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000010">
        <property type="entry name" value="probable methyltransferase TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025806">
        <property type="entry name" value="TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056921">
        <property type="entry name" value="TARBP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25050">
        <property type="entry name" value="TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51624">
        <property type="entry name" value="SAM_MT_TRMH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 16" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TARBP1" evidence="14">
        <location>
            <begin position="212"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="13">
        <location>
            <begin position="1433"/>
            <end position="1574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013914844.1"/>
        </source>
    </evidence>
    <sequence length="1592" mass="181528" checksum="5F199CB1B39B5F7B" modified="2020-10-07" version="1">MDAVLADTLLSQCPDWGACLRSVCGEKGALTLERAEALRFLLERATRVGAGNRGEEAQRAARELALHRCLPALLSGSGVPAGESPEGWRRFVTATGGVLRSCILVCGPRLAERLAGEVLETCAGIQALQMEGSIEVLCAAAPWLEDPALLRRTVAAALKLLRGEGEEREEEKALLVGGRLLPALGRNGAALSLIWEGLVLSPLADAPRVNRTLLVLSALADVLFPRLDGVTDGEAASLLDARLSCKFWEVVQKGLSESDALCRKRARYLLKKAVDVSDKVALECKCSLDDGNGSYLFWWCAEKKDQFTQFWENYILIMETLEGNQIHVIKPVLPKLNSLYELAISEEKGCWMFHPSWHMCIYKRMFESENKTLAKESVIHFLEIWQRKCLPSSQGFSEFIIGPLMDALSESSLYSRVSGQIMGTCPPLGIILQNFMAIFFTSLPESEKRSFLLNFIQKMSNRHWCAIPVLFLSMALAHIPQCRALDSKGLLALRNVLQCSMITHQILLRGAAQCFLLKAAIHSIDTEKVSLSDISNFITYLKPEESLGRRTALWRMLCDWLHVNDKYFQKTLNCEVPQAKTALSVYVQLLVEDYLKTPLTNSEDCLPDWFDAKRVATMILLAADVEEIRHTYNDNTEQIGLKALLNPLLDVLLKLGTNPYISSLKIDKSLQLLLKIMQTCSIKTSRPEVDEIIVFLQKSIMLATESILDFLLRRLTANELNNMSHLEHCNLYLQVLSELMNFYSASEWRKRQFLCTFVSSLINTSLYNLQEIHDNEDIKLEKQIQKVVSLAALRVVCEIMDQKPDLQLQFLPALDSLKTAIFSTQLNHVLKKPNNMEQNSSVEEAASHEGWGKIVARYIHDQWVCLHFVLNNYQNLSKDKHLEQHFYDVQDYKRMLQSALEAIMILSSDHVLPVFHCMKIFVSKLMESSESLCIEAFEMSWKIIFSLSNTQLIFWPNLKAFIQLVFDPEILVTAAKFKREGYLKIKEIIFQMIELSSTKTGIFNVLLSHCCQSWLFPPGEITTVENAFSNAGNYIELLIEACLFGTIFRRDQRLVQEVYAFVENLGDQCAANVVTESKNRDDHYVRVCAIKFICLLDESNKQHKKFIGDFVMKLLDKGELMSKSKVRYYVNSLQHRIENRLWQTLLVLFPKLDEDILSRIVDRILQAGHNNNQASVKYLIEWNIILILHKFPHYLQKFWNCFNYDEDQFKTSICTFLAVLSHFDIILQNTSEKVSALKKALIVVLQWCFHHNFSIRLYALIALKKIWGMCKASHVNDFDTLTSVIESSLNQVELMHGTGNAKKNWQRIQKHFFFEYFHPLKDYCLETIFYTLPRLSETPEEEWISLSKFTSFRDIPLSSTFPSCHSQTTLLDLKPNDWTQQDIGSNLAEPNNERRWMNVQKKIIPCKASGSDLDLELIFQDRATKLGKSNRKLIVVASLIDKPTNLGGLCRTCEIFGVSALTVSNLHYINDKQFQHLSVSAEQWLSVIEVKPFQLIEYLEQKKTEGYTIIGVEQTAKSCNLMEYCFPEKSLLLLGNEHEGIPANVIQHLDTCVEIPQQGVIRSLNVHVSGALLIWEYTRQQMAKEKEQRQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XHV0</accession>
    <name>A0A6I9XHV0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TAF5L</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915309.1">
        <property type="nucleotide sequence ID" value="XM_014059834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XHV0"/>
    <dbReference type="GeneID" id="106543757"/>
    <dbReference type="KEGG" id="tsr:106543757"/>
    <dbReference type="CTD" id="27097"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000202">
        <property type="entry name" value="TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000325">
        <property type="entry name" value="TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF6">
        <property type="entry name" value="TAF5-LIKE RNA POLYMERASE II P300_CBP-ASSOCIATED FACTOR-ASSOCIATED FACTOR 65 KDA SUBUNIT 5L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="9">
        <location>
            <begin position="66"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="264"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="380"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="422"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="464"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="506"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013915309.1"/>
        </source>
    </evidence>
    <sequence length="589" mass="65939" checksum="6EBCD65A63E75E01" modified="2020-10-07" version="1">MKRVRTEQIQMAVNCYVKRRQYVDSEGPLKQGLRLCHTAEEMAANLTVQSESGCTNVISAAPCLAEPQQYEVQFGRLRNFLTDSESQHSQEVMPLLYPLFVYLHINMVQNGLKSTVDSFYSRFHGMFLQNASQKDIIEQLQTTLTIQDILSNFKLRAFLDNKYVVRLQEDSYNFLLRYLQSDNNSALCKVLSLHIHLDVQPAKRVDYQLYASGGSSRSESNGLEPTDMPASILQNEAALDLLQDSIKRVKDGPPSLTTICFYAFYNTEKLLNTAAISPNGKLLAAGFDNSCVKLWSLRPKKLKSEPHLVDVSRIHLACDILDEEAEDDDDIGTEMKLLRGHSGPVYSTSFLSDSSGLLSCSEDTTIRYWDLGCFTNTVLYQGHAYPVWDLDISPCSLYFASASQDRTARLWSFDRTYPLRIYAGHLADVDCIKFHPNSNYLATGSTDKTVRLWSTQQGNTVRLFTGHRGPVLSLAFSPNGKYLASAGEDQRLKLWDLASGTLYKELRGHTDNITSLTFSPDSSLIASASMDNSVRVWDIRNTYCNAPVDGSSSELVGIYTGQISNVLSVQFMACNLLLVTGIAQENQEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XJ27</accession>
    <name>A0A6I9XJ27_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SHC SH2 domain-binding protein 1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106542746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013914041.1">
        <property type="nucleotide sequence ID" value="XM_014058566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XJ27"/>
    <dbReference type="GeneID" id="106542746"/>
    <dbReference type="KEGG" id="tsr:106542746"/>
    <dbReference type="OrthoDB" id="5978115at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013914041.1"/>
        </source>
    </evidence>
    <sequence length="92" mass="9770" checksum="01BCE0001C1FD006" modified="2020-10-07" version="1">MSDSSRGSLGGINIKVLPAPKLKMKNNHIYSNNGYGVTIMKPSEQLGTTADETMECAAAGDGEDSVSKLMQELTLQINANTLEDIKGISLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKA0</accession>
    <name>A0A6I9XKA0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Latent-transforming growth factor beta-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Latent-transforming growth factor beta-binding protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transforming growth factor beta-1-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LTBP1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with TGFB1; associates via disulfide bonds with the Latency-associated peptide chain (LAP) regulatory chain of TGFB1, leading to regulate activation of TGF-beta-1. LTBP1 does not bind directly to TGF-beta-1, the active chain of TGFB1. Interacts (via C-terminal domain) with FBN1 (via N-terminal domain). Interacts with FBN2. Interacts with ADAMTSL2. Interacts with EFEMP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the LTBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013914431.1">
        <property type="nucleotide sequence ID" value="XM_014058956.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106543053"/>
    <dbReference type="KEGG" id="tsr:106543053"/>
    <dbReference type="CTD" id="4052"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000003">
        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000198">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000205">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000004">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000019">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000046">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1 isoform x2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000012">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000515">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000014">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000077">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000001">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000056">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000115">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000273">
        <property type="entry name" value="Putative latent-transforming growth factor beta-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF28">
        <property type="entry name" value="UROMODULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026780164" description="Latent-transforming growth factor beta-binding protein 1" evidence="20">
        <location>
            <begin position="23"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="387"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="22">
        <location>
            <begin position="548"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="617"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="22">
        <location>
            <begin position="668"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="864"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="987"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1028"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1072"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1113"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1155"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="22">
        <location>
            <begin position="1257"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1377"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="22">
        <location>
            <begin position="1434"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1572"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="741"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_013914431.1"/>
        </source>
    </evidence>
    <sequence length="1627" mass="179040" checksum="EA509481D6F42988" modified="2020-10-07" version="1">MAWIRWAFLFCACAFWCPGLDASEKAKVRTVTYVLQPGASQSRWQSGRQQQRLFNLELADSPYGNSWRRSRRSSSLERTRRIIKPGNPGVQLRLGPTVRLQHKPVGIHTAASSSSSTSFARAGKIVLRSKTQQRQSNGTAGAKIHPKAHLRQLQGVNVCGGQCCHGWSKVPGSQRCTKPNCSPPCQNGGMCLRPQLCVCKPGTKGSICEDATKQETVSPGGSSPATPPWPIPQRSVQQMFSKKVQIHQKPNPMAQMTFTLKQKPPGLSQQAQPQIIPLSSQSLMMNVHHGQTHEYVIKPKYYPSKKVLSGEQSTETSLPLRMSQDQLATSYQMGNHTGRIKVVFTPSICKVTCKKGTCQNSCEKGNTTTLISENGHAADTLTAPNFRVVICHLPCMNGGQCSSRDKCQCPPNYTGKLCQIPVQSGSTSQLYHNSQQVSKTVGSHVIHSTHTLPLTVPGQQGAKVKIPPNIVNIHVKHPPEATLQIHQVSRIDSPSAGQKTKTKLPTSGHSQSPYQSLSFQKTQKIHSPYIHQQPITHTFPVSAKTQLGRCFQETVGTQCSKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKPAYFGYSPVVDCPQGYKRINATFCQDINECQLQGVCPNGECLNTMGSYRCTCKLGFVPDPTLSRCILDNPMVTEEKGPCYRFVSAGKQCMHPLSVQLSKQLCCCSVGKAWGPHCEKCPLPGTAAFKEICPGGMGYTVSGSHRKYHHPAVRVHPPVSGKTQITHPVAKGPSPPPLPAKEEPVEALTISQKSEPEMAVREVVEKTSPPLPVEVGPDVSTSSASQVIAPTQVTEINECTVNPDICGQGHCINLPVGYTCLCYDGYRLNDQQTKCSDINECSQTPHLCSLGRCENTEGSFLCICQAGFMANEQGTDCVDVDECARPDTCGEGLCKNTIGSYKCEYCDRGFQRNRRGQCEDIDECSVPHTCPDAECINAPGSYQCVPCRDGFRAWNGKCHDVNECQYSSLCTNGQCENLEGSFRCLCSQGYQLSPAGDQCEDVDECQHQEQQQLCINGQCRNVAGSYRCICEQGYQLSPARDYCEDINECQEDKNVCRGGTCINTKGSYECTCPEGFQLMPSRGCQDINECAGSNLCSSHGKCLNTEGSFRCICEQGFMVSRDGQTCEDIDECANSTICGDHGICENSPGSYRCLCYQGYQYLQDGQGCVDVNECEMLSGVCGEALCENVEGSFLCLCSNENQEYDPMSGLCQFRSSTEIPDRTKETVEKKECYYNLNDANFCDNVLTSNVTKQECCCTLGAGWGDNCEIFPCPVLGSAEFIELCPEGKGFIPSEEPHYGDVAHSYKDADECQLFGQEICKNGFCLNTQPGYECYCKQGTYYDPIKLQCFDTDECQDINSCIDGQCINTEGSYSCFCTHPMVLDSTGKQCIRPAESSEQTEESDVYQDLCWQHLNEDFVCSQPLVGKQTTYTECCCLYGDAWGMQCALCPMKNSEDYAQLCNIPVPGSRPYGQDALVDFEQEHYTSETDPYFMEDRFLNSFEELQAEECGILNGCENGRCVRVQEGYTCDCFDGYHLDMTKMTCVDVNECNELNNRMSLCKNAKCINTEGSYKCVCLPGFVPSNRPNYCTPLNSDPESDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKF2</accession>
    <name>A0A6I9XKF2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide repeat protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TTC16</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013914486.1">
        <property type="nucleotide sequence ID" value="XM_014059011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKF2"/>
    <dbReference type="GeneID" id="106543101"/>
    <dbReference type="KEGG" id="tsr:106543101"/>
    <dbReference type="CTD" id="158248"/>
    <dbReference type="OrthoDB" id="1926212at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45153">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45153:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013914486.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="34020" checksum="188C0C78E19EACA6" modified="2020-10-07" version="1">MTLPSTVTSRAATRRRCCYSTRRSKGRRTSSASTSTEGVGGEDASASPPPPLLLQAFHDPDCFLKLGQLNFAKADYQQALELCPLDPKLRHRIAWLSNETGLQDFREKQYLLAETHFSSAVDNDPWEVKYYLNRAKTRVFLQEFLGAKEDMISALLLNPNKEEVRSLMASLFPGESAESLLSSKVGDLTKTLLDRKLKSFPGGEALQRPLKSAGSEPPTNEYVQIVNQQAETSVAKEEEVCEEKKLQKRLAECQQSSHQANEELNETRQNKPTLESKTIWVEPCSRPPPKECPKEPYCWRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XKG5</accession>
    <name>A0A6I9XKG5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methenyltetrahydrofolate cyclohydrolase</fullName>
            <ecNumber evidence="2">3.5.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MTHFD2L</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.9" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013914432.1">
        <property type="nucleotide sequence ID" value="XM_014058957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XKG5"/>
    <dbReference type="GeneID" id="106543055"/>
    <dbReference type="KEGG" id="tsr:106543055"/>
    <dbReference type="CTD" id="441024"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000070">
        <property type="entry name" value="probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF7">
        <property type="entry name" value="BIFUNCTIONAL METHYLENETETRAHYDROFOLATE DEHYDROGENASE_CYCLOHYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="8">
        <location>
            <begin position="51"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="9">
        <location>
            <begin position="185"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013914432.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="37311" checksum="A8297B78620C149D" modified="2020-10-07" version="1">MAATSVPSRLLLLRGRRRGGFFLLPGVRNISKNCSRGNCPSNGSRNEATIISGRRLAAQVFEEIQRDIESWLTLGHKRPHLTVVLVGENPASHTYVRNKVKAAAAVGICSEIILRPKEISQEELLDLTGKLNKNSRVSGILVQLPLPDHIDERTVCNSVAPEKDVDGFHIFNIGRLCLDQPSIIPATAAAVWEIIKRTGIQTFGRNVVVVGRSKNVGMPISMLLHSDGEHERPGGDATVTITHRYTPKDQLMIHTQLADIVIVAAGIPKLITSDMIKRGAAVIDVGINHIHDPVTGKTKLVGDVDFEEVKKKASFITPVPGGVGPMTVAMLLKNTLIAAKKLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XLN0</accession>
    <name>A0A6I9XLN0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106540950</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013911743.1">
        <property type="nucleotide sequence ID" value="XM_014056268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XLN0"/>
    <dbReference type="GeneID" id="106540950"/>
    <dbReference type="KEGG" id="tsr:106540950"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF7">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="120"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="80"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013911743.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28884" checksum="3B038DB97E673427" modified="2020-10-07" version="1">MVVTRDRNEESLQVVSMEGQETVVHHPSTKQIYSFNFDFSFWSFDKGSNYASQEKIYRSLAMPLLETAFEGYNACLFAYGQTGSGKSYTMMGFDDDERGIIPRFCQDLFHQIAEKETEQNWLQLGNKHRATAATGMNDKSSRSHSVFTLVMTQTKTEIVEKKEYEHRIISRVNLIDLAGSERCSTGRTSGERLKEGASINKSLLTLGKVISALSEQNQSRKKLFVPYRDSVLTCLLMVQPFNVVDVEEDNGKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XN77</accession>
    <name>A0A6I9XN77_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106543797</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013915351.1">
        <property type="nucleotide sequence ID" value="XM_014059876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XN77"/>
    <dbReference type="GeneID" id="106543797"/>
    <dbReference type="KEGG" id="tsr:106543797"/>
    <dbReference type="OrthoDB" id="9011197at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013915351.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="35492" checksum="5AC37DD6C1F0EA2F" modified="2020-10-07" version="1">MIKQNQTQVTEFIFSGFTDHPELQIVLFNIFLMIYIITLSANLGMILLIKIDPQLHTPMYFFLSHLAFLDASYSSTVTPKAIADFLADKKTISWAGCLIQFFMFVALVTTELFLLSAMAYDRYVAICHPLLYSRIMSKKCCVSLVVGVYSYGFVNSIIQTALTFQLSFCGPNLINHFYCNDPPLLALSCSNTQPKEIQLFALSTINLTGSLFTVIISYIYILNAIFRKPSTSGRHKAFSTCASHLTAIVIFYGTLFFMYVQPSSSHSLNYDKVISVLYAVVIPMLNPLIYVLRNKEVKGAWRRIKIKKLFFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XPL4</accession>
    <name>A0A6I9XPL4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6</fullName>
            <ecNumber evidence="15">3.6.1.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phospholipid phosphatase 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27 28" type="primary">PPAPDC2</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27 28">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(2E)-geranyl diphosphate + H2O = (2E)-geranyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47944"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58057"/>
            <dbReference type="ChEBI" id="CHEBI:88107"/>
            <dbReference type="EC" id="3.6.1.68"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:47945"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2E)-geranyl phosphate + H2O = (2E)-geraniol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:68020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17447"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:88107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:68021"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(2E,6E)-farnesyl diphosphate + H2O = (2E,6E)-farnesyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:88226"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:48129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2E,6E)-farnesyl phosphate + H2O = (2E,6E)-farnesol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:48132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16619"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:88226"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:48133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + H2O = (2E,6E,10E)-geranylgeranyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68008"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:144936"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:68009"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(2E,6E,10E)-geranylgeranyl phosphate + H2O = (2E,6E,10E)-geranylgeraniol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:68016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46762"/>
            <dbReference type="ChEBI" id="CHEBI:144936"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:68017"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphate + H2O = 1,2-dihexadecanoyl-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43236"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:72859"/>
            <dbReference type="ChEBI" id="CHEBI:82929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:43237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>presqualene diphosphate + H2O = presqualene phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67968"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57310"/>
            <dbReference type="ChEBI" id="CHEBI:176803"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:67969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>presqualene phosphate + H2O = presqualene alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:68024"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:176803"/>
            <dbReference type="ChEBI" id="CHEBI:176962"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:68025"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.68" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013915961.1">
        <property type="nucleotide sequence ID" value="XM_014060486.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013915962.1">
        <property type="nucleotide sequence ID" value="XM_014060487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XPL4"/>
    <dbReference type="GeneID" id="106544256"/>
    <dbReference type="KEGG" id="tsr:106544256"/>
    <dbReference type="CTD" id="403313"/>
    <dbReference type="OrthoDB" id="10266771at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03391">
        <property type="entry name" value="PAP2_containing_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF18">
        <property type="entry name" value="POLYISOPRENOID DIPHOSPHATE_PHOSPHATE PHOSPHOHYDROLASE PLPP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="25">
        <location>
            <begin position="133"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013915961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_013915962.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29138" checksum="F5E5BBA783539890" modified="2020-10-07" version="1">MPSPRNSPKSIRERRAYDSRLEFLSLTNQKGPISPENVSHHKDSPTSTGTQQALPEEDCMKLNPSFWSIALHSLLAVDLWASKLLGVCAGENSPWGSVRPLMMVIEVSGHGIPWLTGTLYGLYRSGSSAGREVLLNLLFALILDLVLVAAVKGLVKRRRPVHNKMDMFATLSVDKYSFPSGHATRAAMVCRFILHHLVLAIPLRVLVVLWTFIVGISRVMLGRHNVTDVVFGLSMGYMQYNLVEYFWLSPVNAPVLFVLWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XQN4</accession>
    <name>A0A6I9XQN4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Ventral anterior homeobox 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">VAX1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013913018.1">
        <property type="nucleotide sequence ID" value="XM_014057543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XQN4"/>
    <dbReference type="GeneID" id="106541956"/>
    <dbReference type="KEGG" id="tsr:106541956"/>
    <dbReference type="CTD" id="11023"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000375">
        <property type="entry name" value="Ventral anterior homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050877">
        <property type="entry name" value="EMX-VAX-Noto_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339">
        <property type="entry name" value="HOMEOBOX PROTEIN EMX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339:SF32">
        <property type="entry name" value="VENTRAL ANTERIOR HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="97"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="99"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="211"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013913018.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="33639" checksum="CAEAB9FBAF23099C" modified="2020-10-07" version="1">MFGKQDKMDVRCNSELETSRLSKNGHKEGKESKSSEGNISNSFLKEPQGTFSASGVSEDCSKNKSSSADPDYCRRILVRDAKGSIREIILPKGLDLDRPKRTRTSFTAEQLYRLEMEFQRCQYVVGRERTELARQLNLSETQVKVWFQNRRTKQKKDQGKDSEMKSVVSETAATCSVLRLLEQGRLLSPPGLPGLLPPCATSALGSALRAPSLASRTGGSGSGTAGDSPHHPAVSSAAAGPAHPQPGGHNLFSLPVPTLLGSVASRLSSNPLAMAGSLAGNLQELSARYLSSSAFEPYSRTAGKESAEKKALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XS69</accession>
    <name>A0A6I9XS69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106537841</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907585.1">
        <property type="nucleotide sequence ID" value="XM_014052110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XS69"/>
    <dbReference type="GeneID" id="106537841"/>
    <dbReference type="KEGG" id="tsr:106537841"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF16">
        <property type="entry name" value="ANOCTAMIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="10">
        <location>
            <begin position="15"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="9">
        <location>
            <begin position="157"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013907585.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="30607" checksum="F310227540E28D31" modified="2020-10-07" version="1">MPFRKKCYYTDWRSKTMGSLQRNMRELKSWLPRNPMKLDKEALPDLEETDCYTAPFSRARIHHFTINNKDSFFSNSTRSRMVHHMLQRTKYEDGKPKMGINRLLSNGTYEAAFPPHEGSHKSRHPIKTHGANNHRHLLYERWARWGMWYKYQPLDLIRRYFGEKIGLYFAWLGWYTGMLIPAALVGLCVFLYGLFTMDSSQVSKEICEANDTIMCPMCEKSCTLQKLNESCIYAKVTGFSMIIGFCFPQGFCDPARWAKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSF2</accession>
    <name>A0A6I9XSF2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Very long-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="17">1.3.8.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">ACADVL</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="35">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a very-long-chain 2,3-saturated fatty acyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = a very-long-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:19181"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:83724"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="EC" id="1.3.8.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:19182"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>dodecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47296"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:47297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>eicosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-eicosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:47237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>octadecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-octadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:71412"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:47241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H(+) = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:43449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>tetracosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetracosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:74693"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:47233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:47317"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 28">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homodimer. Homodimerizes after import into the mitochondrion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 28">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.9" evidence="17"/>
    <dbReference type="RefSeq" id="XP_013913613.1">
        <property type="nucleotide sequence ID" value="XM_014058138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSF2"/>
    <dbReference type="GeneID" id="106542417"/>
    <dbReference type="KEGG" id="tsr:106542417"/>
    <dbReference type="CTD" id="37"/>
    <dbReference type="OrthoDB" id="2588832at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017099">
        <property type="term" value="F:very-long-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01161">
        <property type="entry name" value="VLCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000008">
        <property type="entry name" value="acyl-CoA dehydrogenase family member 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000017">
        <property type="entry name" value="very long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000006">
        <property type="entry name" value="very long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000001">
        <property type="entry name" value="Very long-chain-specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049448">
        <property type="entry name" value="ACAD9/ACADV-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF11">
        <property type="entry name" value="VERY LONG-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21343">
        <property type="entry name" value="ACAD9-ACADV_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 28" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 28" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 28" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="32">
        <location>
            <begin position="78"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="31">
        <location>
            <begin position="192"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="30">
        <location>
            <begin position="305"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="ACAD9/ACADV-like C-terminal" evidence="33">
        <location>
            <begin position="505"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="35">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF21343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_013913613.1"/>
        </source>
    </evidence>
    <sequence length="632" mass="69010" checksum="0AD3A37DFD589929" modified="2020-10-07" version="1">PRDEGGTPSIPTYVPLSPFFFQAVPEKQRPKAESLSQEELGKKHQSTETKSFAVGIFKGQIITEQVFPYPSVLNEEQTQTLRELVDPVARFFEEMNNPSRNEELGHVQEKTMRGLKDMGAFGLQVPTAFGGLGLTNTQYARMVEIVGMHDLGVGITLGAHQSIGFKGILLYGSREQKKKYLPKLATGETIAAYCLTENTSGSDAASIRSRAELSPCGTYYTLNGSKLWISNGGIADIFTVFAKTPQQDETGKMKDKITAFIVERSFGGVTSGPPEKKMGIKASNTAEVHFDNVRVPVENVLGAPGAGFKVAMNILNNGRFGMAAAMAGTMRALIHKAVDFAANRTQFGEKIHTFGGIQEKLARMALLHYVTESMAYMISANMDRGASDFQIEAAISKIFGSEAAWSVSDECIQTMGGMGFMKEGGVEQVMRDLRIFRIFEGTNDILRLFIALYGFQNAGNQLRGLQQAIKNPFGNAGLLVSEAGKRVRRKAGLGTGITLKGVVHPSLESSSEQAVQAIDLFAAGIENQLLKHGKKVVEEQFMLKQIADSAIDIYAMVVVLSRASRALEQGQPTAEHEKVLCETWCMEAYKRVTQNLISLPSSNTQQMFKNFRVISKAMVEKGGVVSPYTLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSI6</accession>
    <name>A0A6I9XSI6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lamin-B2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LMNB2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus lamina</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916706.1">
        <property type="nucleotide sequence ID" value="XM_014061231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSI6"/>
    <dbReference type="GeneID" id="106544838"/>
    <dbReference type="KEGG" id="tsr:106544838"/>
    <dbReference type="CTD" id="84823"/>
    <dbReference type="OrthoDB" id="102442at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005652">
        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090435">
        <property type="term" value="P:protein localization to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721">
        <property type="entry name" value="LAMIN DM0-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721:SF2">
        <property type="entry name" value="LAMIN-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="10">
        <location>
            <begin position="115"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="9">
        <location>
            <begin position="243"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="8">
        <location>
            <begin position="403"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="48"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013916706.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="48996" checksum="960C051F0941E549" modified="2020-10-07" version="1">MPAELSTGSRGPEGSPGGSYKKKEGEWSTSQGRIRELEVLFHRSEAELTATLSEKQSLEAEVADARAQLAKVQREIELHSRLHHRNIVGFRRHFADQENIYLVLEYCSRKVSTAEERMRDLEELMAGEQEKLRKLLDAKEREMTDMREQMQQQLMEYQELLDVKLALDMEISAYRKLLEGEEERLKLSPSPPSRLTVSRAASSSSISGTRSLRSKRKRAEAEELPGQTPSGSARGGASTGSNLQVSQQASAKGSISIEGVDPEGMFVQLKNHSDKDQSLGSWKLKRKIGEGGEIAYKFTPKYVLRAGQTVTIWAAEAGVAHSPPSVLVWKNQGSWGTGDQIHTCLVNSEGEAPKEEAKEGSRDPMEDPSCLSPLLLQRGSLRSRLPEVEELPEGLRGAHPAHMATKWVDYSNKYGFGYLLSNGSTAVLLPDGTHMALCPQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XSY1</accession>
    <name>A0A6I9XSY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Solute carrier family 41 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106545226</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917192.1">
        <property type="nucleotide sequence ID" value="XM_014061717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XSY1"/>
    <dbReference type="GeneID" id="106545226"/>
    <dbReference type="KEGG" id="tsr:106545226"/>
    <dbReference type="OrthoDB" id="5791097at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000001">
        <property type="entry name" value="Solute carrier family 41 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000002">
        <property type="entry name" value="Solute carrier family 41, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF22">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="11">
        <location>
            <begin position="123"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="11">
        <location>
            <begin position="336"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013917192.1"/>
        </source>
    </evidence>
    <sequence length="496" mass="54228" checksum="DF09070B46DAE27E" modified="2020-10-07" version="1">MRRTPELRKINMKGEETRQRRKEGRTRNGERSRPKAKSKSKHKESKLGSQGTEDGPLLLPQKQPPEAKVAMAKLQEESTLAIGLQVLLPYLLAGMGMVLAGMVLDYIQHWKVFIKIREMFILIPALIGLKGNLEMTLASRLSTAANTGRMDNTQEKWKMAICNMALIQVQATVIGLLAAVAAILLGALSNGYLELNHAVVLCASSLTTAFVAAFFLGLLMIGVIIGARKIGINPDNVATPIAASLGDLITLSLLAGISSILFRYIDVTYLSPMVCTIFIVLIPLWIAIARQSPPVAEVLKTGWQPIIIAMIISSLGGVILDKTVAKPNFEGIAVFVPVINGVGGNLVAIQASRISTFLHFWSMPGVLPQKMRQHWPNPCTTFFASGINSKSARVLMLLVIPGHLMFLYAIHFFEGGHTAFTFTFVAFYLIAALLQVGILLYMADIIVRLMWRKGLDPDNYSIPYLTALGDLLGTGFLAVCFYLLWLLSGLEPKLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XT21</accession>
    <name>A0A6I9XT21_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein C11orf80 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106538030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013907875.1">
        <property type="nucleotide sequence ID" value="XM_014052400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XT21"/>
    <dbReference type="GeneID" id="106538030"/>
    <dbReference type="KEGG" id="tsr:106538030"/>
    <dbReference type="CTD" id="79703"/>
    <dbReference type="OrthoDB" id="9424252at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028040">
        <property type="entry name" value="TopoVIB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14652">
        <property type="entry name" value="TYPE 2 DNA TOPOISOMERASE 6 SUBUNIT B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14652:SF2">
        <property type="entry name" value="TYPE 2 DNA TOPOISOMERASE 6 SUBUNIT B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013907875.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="14116" checksum="C931C7D24AD4CB68" modified="2020-10-07" version="1">MLEGTIAVSLDIQDSPQRVNQLHCAAIIAAKGDLCRKLLSDLTFKEIESLLPFCQVGVLHGSGCPHESLLTMPFQLSFQLCEKSEILKEDSITLKQFIHRISLVHTTIKFHYCVKINGNTSAETYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTC5</accession>
    <name>A0A6I9XTC5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dedicator of cytokinesis protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106538117</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013907990.1">
        <property type="nucleotide sequence ID" value="XM_014052515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTC5"/>
    <dbReference type="GeneID" id="106538117"/>
    <dbReference type="KEGG" id="tsr:106538117"/>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000004">
        <property type="entry name" value="Dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF6">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOCKER" evidence="4">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="408"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013907990.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="66665" checksum="BA7F9E71174891FC" modified="2020-10-07" version="1">MQTEFQSSQTHRHLKEHLYEMIIEYFDKGKMWEEAIGLCKELAEQYEMEVFDYELLSQNLIQQAKFYENIMKILRPKPDYFAVGYYGQGFPTFLRNKMFIYRGKEYERREDFQAQLMSHFPNAEKMNTTAPPDEKLRNAAVQHIQCFTVQPVLEEQLRFKNKPVPDQVINFYKSNYVQRFHYSRPTRKGSVDPENEFASMWIERTSFTTAYKLPGILRWFEVVSMSQTTISPLENAIETMSMTNEKILTMINQYQSDENLHINPLSMLLNGIVDPAVMGGFAKYEKAFFTDEYARHHPQDQEKLNRLKDLIAWQVPFLAAGIKIHERRVSDSLRPFHERMEECFRHLKVKVEKQYGVRELPDFDDRRLGRPKSMLRSYRQMSIISMSSMNSECGTPNKTAGESFELEVMSPKATESVSEENSRSISRQPEVKMRKGRKKEKRSSVILPDKKSSDLPDMKCLSRKYEFMSDTNLSEHLDVPQKTSVLLKQMSFASRSMPTIPALTLSICPTLEETNVSQKISQTSIPPILETEKKTLKRKKVNQLFKTIYKSKQPEDGRCTSERQSDVSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XTF5</accession>
    <name>A0A6I9XTF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NACHT, LRR and PYD domains-containing protein 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106542698</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913978.1">
        <property type="nucleotide sequence ID" value="XM_014058503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XTF5"/>
    <dbReference type="GeneID" id="106542698"/>
    <dbReference type="KEGG" id="tsr:106542698"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050637">
        <property type="entry name" value="NLRP_innate_immun_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690:SF19">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="33"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013913978.1"/>
        </source>
    </evidence>
    <sequence length="511" mass="60897" checksum="503CD1DD6AA6483A" modified="2020-10-07" version="1">MGRKHREIISKRVESSTSIEALFNPDKHGLIPQVIVLQGAAGIGKTIMAKKIMFDWASQQIYQDKFNYAFYISCSEMNFHAESQKTSIAEVISNEWLKCHEVKNVIQNILKNEEKLLFIIDGFDELRYSFDQPKDSFCIDPWKKEPVRILLSSLFQKKILPKSSLIITTRPMALEKLHQCLEYPRYFQILGFSTKEREEYFYNFFENEDQAMQAFRYVKQNDTLFTMCVIPLVSWIICTVMKQEMERGKDLQKTPYTLTAIYMLYFSSLLKFHHKESKPDVQGNVKSLCSLAEEGIWKQQILFMEEEVKKHSLDQRDYLPLFLNQSVFKRDIDRIQTYNFIHLSFQEFFAALFYVLEEGEEQHSENWNKNLQTLLESHKSFRRDFAVGFRFLFGFLNEEKRMRQLKKEFGWEISPKNKEFLLDWVKNKINRRINFRRQKEILAYLYETQDDNFVNDALCGVTEIIYQCNSDMELMILAYCIQHCQNLKKLLIKSPTFPYRESENLFFSKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XUQ5</accession>
    <name>A0A6I9XUQ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TRAF-interacting protein with FHA domain-containing protein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">TIFA</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TIFA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917792.1">
        <property type="nucleotide sequence ID" value="XM_014062317.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013917793.1">
        <property type="nucleotide sequence ID" value="XM_014062318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XUQ5"/>
    <dbReference type="GeneID" id="106545682"/>
    <dbReference type="KEGG" id="tsr:106545682"/>
    <dbReference type="CTD" id="92610"/>
    <dbReference type="OrthoDB" id="9893545at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033621">
        <property type="entry name" value="TIFA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31266:SF2">
        <property type="entry name" value="TRAF-INTERACTING PROTEIN WITH FHA DOMAIN-CONTAINING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31266">
        <property type="entry name" value="TRAF-INTERACTING PROTEIN WITH FHA DOMAIN-CONTAINING PROTEIN A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="48"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013917793.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="20812" checksum="EF04A8A7EDCFFF18" modified="2020-10-07" version="1">MSDFETQDTEETVTCLQITVYHPNQGEKPVFNALSFCHEQQLRADDIVKFGRDPNICRFHFVDSRVSRVQFCLQFFRHFNSSEFGFEIKNLSKKGKLSVDGVELAYLNKVNLPDKCKVCFGEYQMQLQKQEGQCEDFFNICFELARTPLLQENNLFLKKPVCESGSSYAQFPTEMDDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XW14</accession>
    <name>A0A6I9XW14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein FAM49B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FAM49B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYRI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013920374.1">
        <property type="nucleotide sequence ID" value="XM_014064899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XW14"/>
    <dbReference type="GeneID" id="106547662"/>
    <dbReference type="KEGG" id="tsr:106547662"/>
    <dbReference type="CTD" id="51571"/>
    <dbReference type="OrthoDB" id="60973at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039789">
        <property type="entry name" value="CYRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009828">
        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12422">
        <property type="entry name" value="GH09096P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="5">
        <location>
            <begin position="18"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013920374.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36806" checksum="EE3B7900170F6108" modified="2020-10-07" version="1">MGNLLKVLTCTDLEQGPNFFLDFENAQPTESEKEIYNQVNVVLKDAEGILEDLQSYRGAGHEIREAIQHPTDERLQEKAWGAVVPLVGKLKKFYEFSQRLEAALRGLLGALTSTPYSPTQHLEREQALAKQFAEILHFTLRFDELKMTNPAIQNDFSYYRRTLSRMRINNVPAEGENEVNNELANRMSLFYAEATPMLKTLSDATTKFVSENKNLPIENTTDCLSTMASVCRVMLETPEYRSRFTNEETVSFCLRVMVGVIILYDHVHPVGAFAKTSKIDMKGCIKVLKDQPPNSVEGLLNALRYTTKHLNDETTSKQIKSMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XWR9</accession>
    <name>A0A6I9XWR9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kelch repeat and BTB domain-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KBTBD8</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KBTBD8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013908975.1">
        <property type="nucleotide sequence ID" value="XM_014053500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XWR9"/>
    <dbReference type="GeneID" id="106538871"/>
    <dbReference type="KEGG" id="tsr:106538871"/>
    <dbReference type="CTD" id="84541"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014029">
        <property type="term" value="P:neural crest formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18483">
        <property type="entry name" value="BACK_KBTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18274">
        <property type="entry name" value="BTB_POZ_KBTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000006">
        <property type="entry name" value="Kelch repeat and BTB domain-containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000014">
        <property type="entry name" value="kelch repeat and BTB domain-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000020">
        <property type="entry name" value="Kelch repeat and BTB domain-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028764">
        <property type="entry name" value="BTB/POZ_KBTBD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF433">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013908975.1"/>
        </source>
    </evidence>
    <sequence length="601" mass="68711" checksum="A9452A9641FBEC94" modified="2020-10-07" version="1">MAATGEQSKFTQTLYGTPSSSTAIGGIDPFHACSILQQLKMMYDEGQLTDIVVEVDHGKTFSCHRNVLAAISPYFRSMFTSGLTESTQKEVRIVGVEAESMHLVLNYAYTSKITLTEANVQALFTAASIFQIPSIQDQCAKYMISHLDPQNSIGVFIFADHYGHQELKIRSQDYIRKKFLSVTKEQEFLHLRKDQLVSILDSNDLNVDKEEHVYESIIRWFEHEPSKREDHLPEIFAKCIRMPLLDEAFIEKIPPMFAQAIAQNSVQKEQSGANGYTQRLGMTASEMIICFDAAHKHSGKKQTVPCLDSITGRVFKLCKPPNDLREVGILVSPDNDIYIAGGYRPSSSEVSIDHRAESDFWLYDHSGNKWIPKSPLLRARIGCKLVHCCGKLYAIGGRVYEGDGRNSLKSVECYDGRENCWTAVCPMPVAMEFHNAVEYKDNIYVLQGEFFLCYDPRKDYWGFLTPMTVPRIQGLAAVYNHSIYYIAGTCGNHQRMFTVEAYDIELNKWTRKKDFPCDQSINPYIKLVVLKNKLNLFVRATQVTVEEHVFRTSRKNSLYQYDEANDQWQKVYETPDKLWDLGRHFECVVAKLYPQCLQKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZ26</accession>
    <name>A0A6I9XZ26_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mucin-5AC-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106547058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919567.1">
        <property type="nucleotide sequence ID" value="XM_014064092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZ26"/>
    <dbReference type="GeneID" id="106547058"/>
    <dbReference type="KEGG" id="tsr:106547058"/>
    <dbReference type="OrthoDB" id="10662295at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="602"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="572"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013919567.1"/>
        </source>
    </evidence>
    <sequence length="679" mass="67996" checksum="7F181B074526EDD9" modified="2020-10-07" version="1">MAEAKFIALSEYCSVVTWYVQLLKDLNVKVSDAVTVFEDNAACIKMETGGKGPSTATPDSSSSLPTGTTGTSSSLITSSAPQATSKPPEDSSRHTTAISRTPSSKTTSSARSGAKGALHVSTKLPTATSKTSSSETTSSAPPVSSRTLAATSNVSTVILGTPSSETASSAPPGGTATLDSSSSLPTGTTGTSSSLITSSTPPAASRTPDVSSSVSTIISGTRSSETTSSAPSGTTRLPDTTTTIPAPTSKAPSSAATSRAPPAASGKLHTSTRLPTPTSETPSAETTSRTPPGASRPTDSSSSLPKATSGTTTSQTMTTVHVSASGTPDASSRLSTPISGTPSSITTSSAPSGTTGPPDTSTTLPAATSKTTSPETTSSAPSGTTGPPDTSTTLPAATSKTTSPETTSRAPPGASRPTDFSSSSSLANPAGNPEATLSNRTFSTSLRSPTGSIFPTNPISSNPRSTHFSPIKTNIPTSGISPGLSVNPGSIGPISSHPGLTNSVPLNQDPTIFQASMPTSGISPGLSVNPGSIGPISSHSGLTNSVPSNQDSASFQTSIQTSGISPGVTSNPGSTIPGSSSEPTGSTSDSTVTLKQGDEAALTYWIIILICIVCIGAIFLMLCLSFLFVGKLRSSKGESSMGMLRSLTPIFAYFGQEKKSSAYMFKRPILGKSHGIDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZJ5</accession>
    <name>A0A6I9XZJ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytoplasmic phosphatidylinositol transfer protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106547247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013919817.1">
        <property type="nucleotide sequence ID" value="XM_014064342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZJ5"/>
    <dbReference type="GeneID" id="106547247"/>
    <dbReference type="KEGG" id="tsr:106547247"/>
    <dbReference type="OrthoDB" id="18453at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF78">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CYTOPLASMIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919817.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16720" checksum="EF7F39FE92DF3285" modified="2020-10-07" version="1">MLVKEYRICMPLTTEEYRVGQLYTISKHSHQESDRGEGVEVVKNEPHEDPVHGPGQFTEKRVHLSSKLPSWARAVTPRIFYITEKAWNYYPYTITEYTCSFLPKFSIYIETKYEDNCGNGTNIFLNEKILGDHDVMFLDIAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9XZZ0</accession>
    <name>A0A6I9XZZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Sodium leak channel NALCN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Sodium leak channel non-selective protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Voltage gated channel-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">NALCN</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the NALCN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013919556.1">
        <property type="nucleotide sequence ID" value="XM_014064081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9XZZ0"/>
    <dbReference type="GeneID" id="106547052"/>
    <dbReference type="KEGG" id="tsr:106547052"/>
    <dbReference type="CTD" id="259232"/>
    <dbReference type="OrthoDB" id="10069766at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032224">
        <property type="term" value="P:positive regulation of synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032230">
        <property type="term" value="P:positive regulation of synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000080">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000060">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000061">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000032">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000034">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028823">
        <property type="entry name" value="NALCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46141:SF1">
        <property type="entry name" value="SODIUM LEAK CHANNEL NALCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46141">
        <property type="entry name" value="SODIUM LEAK CHANNEL NON-SELECTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="3" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="568"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="598"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="633"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="693"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="244"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="566"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="968"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="155"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="970"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_013919556.1"/>
        </source>
    </evidence>
    <sequence length="1095" mass="126198" checksum="5858A8A2369781F7" modified="2020-10-07" version="1">MVKISKLPSDFTVPKIRESFMKQFIDRQQQDTTCLLRRLPSASSSSCDHAKRLAIEDKYIDQKLRKSVFSIRARNLLEKESAINKILRACTHQRMLSGSLEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAKSGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHIIAREDCHGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIAVLVLAQSVLLSVKWDDQDPVTGPLAAMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFAITNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDIRTYWKTDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNANNQDNNVQPETSSEQQLLSPTLSDRGGYRQDSGDTGRPQRKFGQWRLPSAPKPISHSVSSVNIKIGGRTTMKSVVCKMNPMSDMASCGSEVKKWWTRQLTVESDESGEDLLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0N0</accession>
    <name>A0A6I9Y0N0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ANXA5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013916503.1">
        <property type="nucleotide sequence ID" value="XM_014061028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0N0"/>
    <dbReference type="GeneID" id="106544681"/>
    <dbReference type="KEGG" id="tsr:106544681"/>
    <dbReference type="CTD" id="308"/>
    <dbReference type="OrthoDB" id="37886at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050819">
        <property type="term" value="P:negative regulation of coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000022">
        <property type="entry name" value="Annexin A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002392">
        <property type="entry name" value="ANX5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF26">
        <property type="entry name" value="ANNEXIN A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00201">
        <property type="entry name" value="ANNEXINV"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013916503.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="26780" checksum="0B39265848F1B141" modified="2020-10-07" version="1">MRPEQIYDAHALKHAIKGAGTDEQVLTEILASRTPAEIRNIKQVYQEEYGADLEDHITSDTSGYYQRMLVVLVQANRDPDGQIKESLIEQDAQELFRAGELKWGTDEEKFITILGTRSISHLRKVFDKYMTISGFQIEETIDRETSGALEKLLLAIVKCVRSVPAYFAECLFYAMKGAGTDDDTLIRIMVSRSEKDLLDIRQAFRRDFAKSLHHVIQKDTSGDYRNGLLLLCGGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y0R7</accession>
    <name>A0A6I9Y0R7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Transaldolase</fullName>
            <ecNumber evidence="4 10">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TALDO1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="4 10"/>
    <dbReference type="RefSeq" id="XP_013919916.1">
        <property type="nucleotide sequence ID" value="XM_014064441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y0R7"/>
    <dbReference type="GeneID" id="106547317"/>
    <dbReference type="KEGG" id="tsr:106547317"/>
    <dbReference type="CTD" id="6888"/>
    <dbReference type="OrthoDB" id="2015515at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000002">
        <property type="entry name" value="Transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00492">
        <property type="entry name" value="Transaldolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009001">
        <property type="entry name" value="PRK12346.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00874">
        <property type="entry name" value="talAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0704">Schiff base</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013919916.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="37537" checksum="258C6FA80321A5C0" modified="2020-10-07" version="1">MSVSPVKKQKMESALEQLKQHTTVVADTGDFHAIEEYKPLDATTNPSLILAAAQMPAYQQLVEDAIAYGKKLGGSEKEQVTNACDKLFVLFGAEILKRIPGRVSTEVDARLSFDKEGMVKRARQFIDLYKEIGISKDRILIKLSSTWEGIQAGKILEEQHGIHCNMTLLFSFAQAVACAEAGVTLISPFVGRILDWYVANTDKKTYEPSEDPGVKSVTKIYHYYKKFGYKTIVMGASFRNTGQIKALTGCDFLTISPKLLGELSKDNSKLTPKLSVKDAQASDLEKVHLDEKTFRWLHNEDQMAVEKLSDGIRKFAADAVKLEVMIKERMFGTANGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y167</accession>
    <name>A0A6I9Y167_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein AF-10-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106547901</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013920642.1">
        <property type="nucleotide sequence ID" value="XM_014065167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y167"/>
    <dbReference type="GeneID" id="106547901"/>
    <dbReference type="KEGG" id="tsr:106547901"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15574">
        <property type="entry name" value="PHD_AF10_AF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000053">
        <property type="entry name" value="protein AF-10 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049781">
        <property type="entry name" value="AF10/AF17_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF93">
        <property type="entry name" value="PROTEIN AF-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="82"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013920642.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15272" checksum="E027EF8671559B39" modified="2020-10-07" version="1">MVSSEPLLLPVSLEGEFSNSMKEMIGGCCVCSDERGWAENPLVYCDGHGCNVAVHQACYGIVQVPTGPWFCRKCESQERAARVKCELCPHKDGALKRTDGGGWAHVVCALYIPEVQFANVSTMEPIVLQSVPHDRYNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y1L8</accession>
    <name>A0A6I9Y1L8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Casein kinase I isoform alpha</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CK1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CSNK1A1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013920356.1">
        <property type="nucleotide sequence ID" value="XM_014064881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y1L8"/>
    <dbReference type="GeneID" id="106547654"/>
    <dbReference type="KEGG" id="tsr:106547654"/>
    <dbReference type="CTD" id="1452"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14128">
        <property type="entry name" value="STKc_CK1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000120">
        <property type="entry name" value="Casein kinase I isoform alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="8 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="7 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="17"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="337"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013920356.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="42707" checksum="23ED19AB922D5B78" modified="2020-10-07" version="1">MASSSGSKAEFIVGNKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKCLESPVGKRKRSMTVSTSQDPSFSGLNQLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQTQTPTGKQTDKSKNNMKGGSIQDSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y209</accession>
    <name>A0A6I9Y209_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WD repeat-containing protein 62-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106545123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013917063.1">
        <property type="nucleotide sequence ID" value="XM_014061588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y209"/>
    <dbReference type="GeneID" id="106545123"/>
    <dbReference type="KEGG" id="tsr:106545123"/>
    <dbReference type="OrthoDB" id="6154712at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052779">
        <property type="entry name" value="WDR62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_013917063.1"/>
        </source>
    </evidence>
    <sequence length="181" mass="20701" checksum="256D4B1335965C63" modified="2020-10-07" version="1">MRRPDASFPDAIAVVFDPRRHWLSCVYKDHSVYIWDVKDIGHARKVWSDLFHSSFVWNVEVYPEFEAHQGCLPPGSFLTCSSDSTIRFWNLENNTHGPFKKNAYSDNLLKVVYVEKGIQYLQDSTNLLERNDNVGYLDVKSGVRVMQISPDGQHLASGDRAGNLSAEDLKKKFVALKVAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y288</accession>
    <name>A0A6I9Y288_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Arylsulfatase G</fullName>
            <ecNumber evidence="11">3.1.6.1</ecNumber>
            <ecNumber evidence="14">3.1.6.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">N-sulfoglucosamine-3-sulfatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ARSG</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Hydrolysis of the 3-sulfate groups of the N-sulfo-D-glucosamine 3-O-sulfate units of heparin.</text>
            <dbReference type="EC" id="3.1.6.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an aryl sulfate + H2O = a phenol + sulfate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17261"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:33853"/>
            <dbReference type="ChEBI" id="CHEBI:140317"/>
            <dbReference type="EC" id="3.1.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.1" evidence="11"/>
    <dbReference type="EC" id="3.1.6.15" evidence="14"/>
    <dbReference type="RefSeq" id="XP_013920626.1">
        <property type="nucleotide sequence ID" value="XM_014065151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y288"/>
    <dbReference type="GeneID" id="106547887"/>
    <dbReference type="KEGG" id="tsr:106547887"/>
    <dbReference type="CTD" id="22901"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033889">
        <property type="term" value="F:N-sulfoglucosamine-3-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.10:FF:000006">
        <property type="entry name" value="Arylsulfatase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000031">
        <property type="entry name" value="arylsulfatase G isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF42">
        <property type="entry name" value="ARYLSULFATASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="17">
        <location>
            <begin position="40"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013920626.1"/>
        </source>
    </evidence>
    <sequence length="538" mass="59524" checksum="C036E290A4F7CBC6" modified="2020-10-07" version="1">MDILLLKGLVLVLVSCFTGLLYYRPTTIFFNWERTVYKKPNFIVILADDVGWGDFGANWDLQKDTPNLDKIAQEGMRFVDFHSAASTCSPSRASLLTGRLGLRNGVTHNFAVSSVAGLPLNETILAEILKEAGYLTAMIGKWHLGHHGPFHPSFRGFDFYFGIPYSPDMGCTDTPGYNLPPCPACPRCSTTVNNHQKNCYPEIALPLFENLTIVQQPVNLTTLAARFTEKVIHFIHYASQKGLPFFLYLALAHMHVPLVLSEAPWNPFCQDPYGTYLKEMDALVGQIKDAVDSTAKEDTLLWFTGDNGPWSEKCDYAGSVGPYTGMWQRRKGGSSAKQTTWEGGHRVPTVAYWPGKIPANVTSASMLSTLDIFPTLVSLANATLPPNRRFDGIDISEVLFGYSSKGHQTLFHPNSGAAGKYGEIQSLRLDQYKAFYLTGGAKACDGSIGQEVHHQPPLIFNLQHDIQEQEPMDIHSAEYRSVLPLVNKALMDILQDIASDNASIADYSHDADAIPCCNPQHLACRCESTYANLSLEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y2C0</accession>
    <name>A0A6I9Y2C0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">TRIM55</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013920666.1">
        <property type="nucleotide sequence ID" value="XM_014065191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y2C0"/>
    <dbReference type="GeneID" id="106547915"/>
    <dbReference type="KEGG" id="tsr:106547915"/>
    <dbReference type="CTD" id="84675"/>
    <dbReference type="OrthoDB" id="5351233at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16760">
        <property type="entry name" value="RING-HC_MuRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000014">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase MID2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000022">
        <property type="entry name" value="Tripartite motif containing 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF17">
        <property type="entry name" value="TRIPARTITE MOTIF CONTAINING 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="540"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="26"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="17">
        <location>
            <begin position="119"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="COS" evidence="18">
        <location>
            <begin position="269"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="331"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="339"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="441"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013920666.1"/>
        </source>
    </evidence>
    <sequence length="585" mass="65974" checksum="182495C27B432DED" modified="2020-10-07" version="1">MSATLNYKSFSKEQQTMDNLEKQLICPICLEMFTKPVVILPCQHNLCRKCASDIFQASNPYLPTRGGTTVASGGRFRCPSCRHEVILDRHGVYGLQRNLLVENIIDIYKQESSRPERKTEQPVCEEHEDEKINIYCLNCEVPTCSMCKVFGAHKDCQVAPLSNVYQRQKSELSDGIAVLVGSNDRIQGIVTQLEETCRTVEECCKRQKDQLCEKFDYLYAILEERKSEMTQIITRSQEEKLEHVRSLIKKYADHLETVSKLVESGIQFMEEPEMALFLQNAKALLQKITEASKAFQMEKIERGYENMTHFTVNLNREEKIIREIYFYREEEDEDEEDAIEGKTPDEIHTESSGEEDEIAERASSQQLQQDIALKDTLAVSQSNLAPSLPSNPLVNTPTTAKQDEIVHSTTAQPAGLVETGLQSNGDSSDPLFYPSWYKASPRQTSNSTPAPLTESRQVGPSIPVEGNVKKTEVPEAASANTNSAVCGKEVNPSTDISQGSLAQSKGVLFLQTYPRVLKAEPLTFLALRHAGVSTIWQMKWLVTMLLVDIGLLRGLTFCISVLAFLILLHHIWNQIQYMVFTLRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y3G7</accession>
    <name>A0A6I9Y3G7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neuropilin and tolloid-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NETO1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013917878.1">
        <property type="nucleotide sequence ID" value="XM_014062403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y3G7"/>
    <dbReference type="GeneID" id="106545750"/>
    <dbReference type="KEGG" id="tsr:106545750"/>
    <dbReference type="CTD" id="81832"/>
    <dbReference type="OrthoDB" id="9971251at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000463">
        <property type="term" value="P:positive regulation of excitatory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000016">
        <property type="entry name" value="neuropilin and tolloid-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF27">
        <property type="entry name" value="NEUROPILIN AND TOLLOID-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026695238" evidence="6">
        <location>
            <begin position="23"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="41"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="172"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013917878.1"/>
        </source>
    </evidence>
    <sequence length="533" mass="60433" checksum="02BBFE75F34D0CB4" modified="2020-10-07" version="1">MIHGRSLFHIVASFTILHWAGATKKGAEKQSSSEGQKTVQCGVWTKYAEGGIFTSPNYPNKYPPDRECVYIIEAAPRQCIELHFDEKYSIEPSWECKFDHIEVRDGPFGFSPIIGRFCGQQNPPKIKSSGRFLWIKFFADGELESVGFSAQYNFTRDPEFKDLGVLKPLPVCEFQMGGSEGIVDSVQIGKEGKASDSEAVDCKWYIRAPPRSKIYLRFLDYEMQNSNECKRNFVAVYDGSSSVEDLKAKFCSTVANDVMLRTGLGVIRMWADEGSRNSRFQMLFTSFQEPPCEANTFFCHSNMCINNTLVCNGLQNCVYPWDENHCKEKTKASLWDQLTNTSGTIIGVTSCIVILLIIISVIVQIKQPRKRFVQKKADFDQTVFQEVFEPPHYELCTLRGTGTSVDLADIADDFENYHKLRRSSSKCIHDHHCGSQLSSTKGSHSNLSTRDTPILTEIQPQPAKPLIQPMNRRNILVMKHSYSQEAADVCEIDEIEEVPTTSHRLSRHDKAVQRFCLIGSLNKHESEYNTTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y4C8</accession>
    <name>A0A6I9Y4C8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Slit homolog 2 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106546413</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013918758.1">
        <property type="nucleotide sequence ID" value="XM_014063283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y4C8"/>
    <dbReference type="GeneID" id="106546413"/>
    <dbReference type="KEGG" id="tsr:106546413"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF383">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 15-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013918758.1"/>
        </source>
    </evidence>
    <sequence length="127" mass="14157" checksum="4C6A53012D546D08" modified="2020-10-07" version="1">MVSIYRRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGVFEGAAAVNELLLTSNRLENVRHKMLTGLESLKTLMLRSNRISCVSNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y5Z2</accession>
    <name>A0A6I9Y5Z2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 115</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM115</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013913575.1">
        <property type="nucleotide sequence ID" value="XM_014058100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y5Z2"/>
    <dbReference type="GeneID" id="106542387"/>
    <dbReference type="KEGG" id="tsr:106542387"/>
    <dbReference type="CTD" id="11070"/>
    <dbReference type="OrthoDB" id="73612at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000004">
        <property type="entry name" value="Transmembrane protein 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013861">
        <property type="entry name" value="TMEM115/Pdh1/Rbl19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377">
        <property type="entry name" value="PLACENTAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08551">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01160">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_013913575.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="39134" checksum="60A78F78B72E7ABA" modified="2020-10-07" version="1">MNRYLPVARQHFLAVLASTSVVVKSICAIVILLYLLSFAVDTVFGLGVTPGYLFPPNFWIWTLATHSLVEKHIWDVGVSLATIVVAGRLLEPLWGALELLIFFAVVNISVGLLGAFAYLLTYMATFSLPYLFSVRIYGMLGFLGGVLVALKQTMGDSTVLKIPQVRMKVVPMLLLLILSVLRLTTLIESNILASYGFGVLSSWIYLRFYQRHSRGRGDMSDHFAFATFFPEILQPVVGLLANLVHTILVKVRVCRKTVKRYDVGAPSSITISLPGTDPQDAERRRQLALKALNERLKRVEDQSAWPSMEDEDEENIKADTPLLPEKSRDSAGVGKASSQESSLITFEDAPSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6I6</accession>
    <name>A0A6I9Y6I6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA replication ATP-dependent helicase/nuclease</fullName>
            <ecNumber evidence="21">3.1.-.-</ecNumber>
            <ecNumber evidence="21">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">DNA2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 27">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="21"/>
    <dbReference type="EC" id="3.6.4.12" evidence="21"/>
    <dbReference type="RefSeq" id="XP_013914060.1">
        <property type="nucleotide sequence ID" value="XM_014058585.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106542767"/>
    <dbReference type="KEGG" id="tsr:106542767"/>
    <dbReference type="CTD" id="1763"/>
    <dbReference type="OrthoDB" id="306218at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071932">
        <property type="term" value="P:replication fork reversal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18041">
        <property type="entry name" value="DEXXQc_DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22318">
        <property type="entry name" value="DNA2_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000721">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000789">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000915">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026851">
        <property type="entry name" value="Dna2/JHS1_DEXXQ-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF433">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 21" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17 21" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 21 26" id="KW-0347">Helicase</keyword>
    <keyword evidence="10 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 21" id="KW-0408">Iron</keyword>
    <keyword evidence="14 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19 21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 21" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="22">
        <location>
            <begin position="50"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="22">
        <location>
            <begin position="135"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="23">
        <location>
            <begin position="383"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="23">
        <location>
            <begin position="487"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="24">
        <location>
            <begin position="562"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU367041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_013914060.1"/>
        </source>
    </evidence>
    <sequence length="810" mass="92096" checksum="16BBB6F91AF7620D" modified="2020-10-07" version="1">MVPYKFPKKMIGFPDTVLQRGLNNRYCVVHVQDDEEREKRLTIVSSMLDDSERELCILQDDWISVPVQPGDIIHLEGECNSGIWIINANSGYLILYPDVLLSGTTVSNSIQCMRRAVLSEKFREVMDPDSFIMQYQLNLEQKDIIHEIEEYLPSFIKWAEVYTYKHSQNNVQLKLXTNNSLSSKSCYNVKVTEILDVEENIWCPRFGLKGKIDVTAEVTIHERSKTQTRIMPLELKSGKESNSVEHRSQVILYSLLCQERRVDPEGGFLLYLKTGNMIPVVGSRMDRRAKCFSLEMFECHDKVCFWNLSHLPGDTVYRLDHEEGALGIEAPLANLSKLMENSPASEKLRSLIVDFYKPQFVQYLSSVLPPEAKGTVANILKGLNKPQKQAMKQVLLSKDYTLIVGMPGTGKTTTICALVRILYACGFSVLLTSFTHSAVDNILLKLVKFKVGFLRLGRAQKVHPDIKRFTEEEICKSKCIKTVAQLEELYQSQPVIATTCMGINHPIFTRKRFDFCIVDEASQISQPICLGPLFYSLRFVLVGDHQQLPPLVQNPEARELGMSESLFKRLEQNKNAVVHLTVQYRMNSKIMSLSNKLVYDGKLECGSEKVSRAVIDLPNLKKLKLEESSYSETWLKETLDPNNPVCFLNTEKVEALEQTEKGCISNMTEARLVFFLTSIFLKAGCKPSDIGVISPYRHQLKIITDLMAGSYLADVEVNTVDRYQGRDKSVIIVSFVRNNVDGNVGELLKDWRRLNVAITRAKHKLVMLGCVPSLCLYPPLKKLLHHLKNEAMIFNIPLGAYEHVLHCKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y6V1</accession>
    <name>A0A6I9Y6V1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibronectin type III domain-containing protein 3B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921043.1">
        <property type="nucleotide sequence ID" value="XM_014065568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y6V1"/>
    <dbReference type="GeneID" id="106548227"/>
    <dbReference type="KEGG" id="tsr:106548227"/>
    <dbReference type="OrthoDB" id="443915at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000185">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF19">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="57"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="151"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="248"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="346"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921043.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="54758" checksum="A58DE29AF6447758" modified="2020-10-07" version="1">MTGPEEVVSEVYHGPDLECTVSNLLPGTTYQFRVRALNDGGYGTYSEAVKITTVAGPPGQCKAPCLTFLSDSSVLVSWESPESFGADISEYRLEWGEDKENLELVYTGTANSFEIDQLSAAAHYFCRLQAINQAGAGSYSDLVSCRAPASVPEAVTILYVSEDEHLDTCPMPVVCLVLNWEEPCNNGSEIVSYNIELGDSILSVGKVTTYVIEDLPPETTFRIRIQAVNEVGAGPFSHFIAAKTRSLPPLPPRLECTAAGPQSLKLKWGESSSRQHTTDDMVYLLQIEDKNKRFTSIYRGPSHTYKVQRLMEYTSYIFRIQAVNGAGEGPFSEVFSFSTTKSIPPVIKAPRVRQLEGNTCEIAWETAPPMKGDPVNYILQVLVGRESEYKQVYKGDETTYQISGLQVNTDYRFRVCVCRRCLDTSQELNGPFSTSVAFILQRSELMLTGELGRTDDPKTKCMMPSDEQFAALLVLGFASVSILFAFILQYLFMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Y9Z9</accession>
    <name>A0A6I9Y9Z9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Solute carrier family 22 member 23-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106547051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013919555.1">
        <property type="nucleotide sequence ID" value="XM_014064080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Y9Z9"/>
    <dbReference type="GeneID" id="106547051"/>
    <dbReference type="KEGG" id="tsr:106547051"/>
    <dbReference type="OrthoDB" id="6884957at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013919555.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="22032" checksum="27C5BCB6D888A8DD" modified="2020-10-07" version="1">MAIERRREEGGGGGRPLPEENGSLPAGEADPAAPVAPPAGGLPAEIQSLPLPTPPSGSCSPLLLDYDGSVLPFLGRLGGGHQKALIFLTWIPALFIGFSQFSDSFLLVHPISRCKGTDGKGNWSEFPPQPPPRGLTTSPSYNHSSSYSRGILAAPELPTPEGKNDSYCHCSERYHRIPAGLQQNIVSKIDGGEMEKFNCRGYSANHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YAW7</accession>
    <name>A0A6I9YAW7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Complement C4-B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106548322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013921145.1">
        <property type="nucleotide sequence ID" value="XM_014065670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YAW7"/>
    <dbReference type="GeneID" id="106548322"/>
    <dbReference type="KEGG" id="tsr:106548322"/>
    <dbReference type="OrthoDB" id="9041528at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006956">
        <property type="term" value="P:complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.20.50.160:FF:000001">
        <property type="entry name" value="Complement component 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF86">
        <property type="entry name" value="COMPLEMENT C4-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013921145.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25460" checksum="00E1285987BAE556" modified="2020-10-07" version="1">MVLSKGDIISVSSIPRGSHLAASIHITPNLMPTFRVVAFYRLGDEIVSNSIWLEVVARCEGKLEIRSSSNVDRLKPQSRVSLTLNTDDKSFVSLSATDAAVYALNGKNRLSQGKVFEAMKSYDLGCTAGGGEDSLGVFADAGLSIRAGEQRSPLRKAHGCEENASRKKRSLSFQQEIDKKGKCWEMRFSIVILGTWLLQFKQRTLLGRARINNRPGRTQIRMEPIKWFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YB39</accession>
    <name>A0A6I9YB39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SH3 and PX domain-containing protein 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SH3PXD2A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH3PXD2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921326.1">
        <property type="nucleotide sequence ID" value="XM_014065851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YB39"/>
    <dbReference type="GeneID" id="106548474"/>
    <dbReference type="KEGG" id="tsr:106548474"/>
    <dbReference type="CTD" id="9644"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06888">
        <property type="entry name" value="PX_FISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12074">
        <property type="entry name" value="SH3_Tks5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12077">
        <property type="entry name" value="SH3_Tks5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12019">
        <property type="entry name" value="SH3_Tks5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12020">
        <property type="entry name" value="SH3_Tks5_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000020">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000031">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000042">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000059">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000141">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000005">
        <property type="entry name" value="SH3 and PX domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037961">
        <property type="entry name" value="SH3PXD2_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035450">
        <property type="entry name" value="SH3PXD2A_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035452">
        <property type="entry name" value="SH3PXD2A_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035453">
        <property type="entry name" value="SH3PXD2A_SH3_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035454">
        <property type="entry name" value="SH3PXD2A_SH3_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="166"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="266"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="447"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="810"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="1045"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="502"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="872"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="914"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="988"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="604"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="645"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="730"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="768"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="872"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="891"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="988"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1000"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_013921326.1"/>
        </source>
    </evidence>
    <sequence length="1106" mass="124765" checksum="C5D07B227212EB29" modified="2020-10-07" version="1">MLSYTVLDANVVDVEKRRNPSKHYVYIIKVTWSDTTCQTIYRRYSKFFDLQMQLLDKFPVEGGQKDPKQRIIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRALVKLPPHISQCDEVFRFFESRPEDLNPPKEDYGSSKRKSVWMSSLSDSPKKAIAGTDASSEPMILEQYVVVSNYEKQENSEISLQAGEVVDVIEKNESGWWFVSTSEEQGWVPATYLESQSGVRDDSEINMSKRGEVSKRRKAHLRRLDRRWTLGGMVNRQQSREEKYVTIQSYTSQGKDEISFDKGVTVDVIQKNLEGWWYIRYLDKEGWAPASYLKKAKDELPTRKKNLTGPVEIIGNIMEISNLLNKKSSSDKETQSEIETTESHVAKKEISLPILCNDSNGNFMMTQDKQISKQVQGSPAIARIAPQRAQISSPNLRMKPPPRRESSLGFQLPKPPEPPSVEVEYYTIAEFQSCISDGISFHGGQKAEVIDKNSGGWWYVQIGEKEGWAPASYIDKRKKPNLNRRTSTLTRPKVPPPAPPNKVKDSEEVTAASTTASGDGQDSPHKQQYEEPEYDVPAFGHDSESELSQGEDGNSDRNSFHLPKPFSGSSLQRAKFRVGESSDDVTHEEETIYENEGFRPYIEDALSSKESSGDSDSRSSSLSLIQINSPHSLSPKSSFLKPKNIQTNFGKSHYSHNEETKPRSASDAGLSSIPKSGTKRDTGELMSSRAKPMVRPKPFLNKADSQSQEMMNISTLRHHLRPTGQLRGGLKGSRSEESESPPQTTSGNSEVSFRRNSTEFITSPSHTLFYAGQLAKTENEGNPSKCLYITTDSYQKVQDSEISFPAGIEVEVLEKQSSGWWYLKYGEIEGWAPSHYLMLFDNQQQENTSSGEDTTSNISQARNRRNESKSNSLEKIEKKVQALNTINQNKRTTPPIPNKPPGGFSKTSGPVKLRNGVRQLAVRPQSVFVSPPPKDNNLPCSLRRNESLSATDHLRAVRRNSSFSAVQSQMNESKVKHDSRLGSERSDNASYISTDRNGIPVSTVRPKPIEKSQFIHNNLKDIYISIADYEGDEESAGFQEGVCMEVLEKNPNGWWYCQIMDSGKPFKGWVPSNYLEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBG5</accession>
    <name>A0A6I9YBG5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dehydrogenase/reductase SDR family member 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106548556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921424.1">
        <property type="nucleotide sequence ID" value="XM_014065949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBG5"/>
    <dbReference type="GeneID" id="106548556"/>
    <dbReference type="KEGG" id="tsr:106548556"/>
    <dbReference type="OrthoDB" id="294295at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF15">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921424.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="15341" checksum="DCA015207379B608" modified="2020-10-07" version="1">MAEEENDEFNFNMWYHFILWLIVIFIWWKWRDGENLDDVDPEGKYIFITGCDSGFGKLAALTFDRRGFQVIAGCLTERGAKELKEESTANLQTVQLDVTNSDNIKQVAEQVKKEVGQRGKQLSPVAGCFSLLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBG9</accession>
    <name>A0A6I9YBG9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">PDZ domain-containing protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PDZD8</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013921481.1">
        <property type="nucleotide sequence ID" value="XM_014066006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBG9"/>
    <dbReference type="GeneID" id="106548609"/>
    <dbReference type="KEGG" id="tsr:106548609"/>
    <dbReference type="CTD" id="118987"/>
    <dbReference type="OrthoDB" id="10004596at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20825">
        <property type="entry name" value="C1_PDZD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00136">
        <property type="entry name" value="PDZ_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21674">
        <property type="entry name" value="SMP_PDZD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039275">
        <property type="entry name" value="PDZD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058801">
        <property type="entry name" value="PDZD8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21519">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21519:SF1">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26547">
        <property type="entry name" value="PDZD8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-LTD" evidence="12">
        <location>
            <begin position="101"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="322"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="10">
        <location>
            <begin position="826"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="511"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="935"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1093"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1018"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="516"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="967"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1101"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013921481.1"/>
        </source>
    </evidence>
    <sequence length="1144" mass="128667" checksum="5A81D92A7D95A328" modified="2020-10-07" version="1">MLSVYGILLSALLGSFLTLLAQLLIFYRRQPEAVPGVSRLRGSNFFVTRVMPDSTLKEYLTGFLVLGQEAFFPGSLISQPSKTEQSFKAPDQESPPLVECTEETLYWLNAICLFLFRELKDTSLLRHWVTRKIKVEFEELLQTKVTGKVLEGVSLRDVSFGNVIPLFKNIKILRPVTCNEEGCPEELDFEVDMEYEGGFHLAIDADLVFGKSAYLFAKISKIMGRLRLVFTRIPFTHWSFAFVDEPLIDLEVKSQFEGRPLPQLTSIIVNQFKKVIKRKHTLPHYKIRLFIFGSYEREINIHCTIELSDKFWEDKERTFIKTAELIKGSSPSIGITLRQIQAKEGEAGHVVIETVTPNSAASAADLQRGDRLLSIGGVRITSTVQVLKLIKQAGERVTIYYERPVGYTPQTSLAQDNLTQLEESTFSDEYLLNEIDNKELDIEFEDLANALKSSEFKDDSSTSPKHIPVSLAGKPPGTLSPVLNRKLHVGSYQASSRVLFKETARPVVHKSPENLDLSQQTNKQPQLNVTKPPVPPRPQIKVPAVSSDVQNKLETGDLSSEKVDKLPFPANNGDKGSEKVIKVIDLGEESTGLKPLAKEASEISSSFKTITNKSEALKESIESLQNIKAPVNKSVTNKSELHKDISDYSPYSLNAKAVENHNSWESPEIPYRNRAGKWARTKASSYIFEVGKEHTYLNIGVWCRDPFKVGGLVCLGYESVKLEDVALDCIATSSMEFIRSFRLNPPAPKATVTRTALRSLISHKGFNEKFCYGDVTLHFKYLREGEPEDSTFLLEKEKENISEEAAAPVLSKEDSYFGQITYTENRHNFQDTQFQNPTWCDYCKKKVWTKAASQCVVCAYVCHKKCQEKCLTDAPFCLGATRRLERVLHPFKFENQDSQSTPGPRVDSELKPANKAAGLTRHLLNTSSRFLNLRQVPKVRVNEQGPEMVEPSPKQTPHPSDNEGSDTDTGGPSSPSKQVTTSGIKLARKEGGLDDSVFIAVKEIGRDLYRSLPTEERIQKLEIMLEKLQQEIDQELESNNSLIREKKEATDVRRKRLLSVALAKSGERLQALTLLMIHYKAGIEDIEENSALELGSRKDDKDEEDNTLVEETDNEEEAVLVMAPVLETLLKEEERLESQNASVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YBT7</accession>
    <name>A0A6I9YBT7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonuclease-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106548680</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pancreatic ribonuclease family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921582.1">
        <property type="nucleotide sequence ID" value="XM_014066107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YBT7"/>
    <dbReference type="GeneID" id="106548680"/>
    <dbReference type="KEGG" id="tsr:106548680"/>
    <dbReference type="OrthoDB" id="8573660at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06265">
        <property type="entry name" value="RNase_A_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.130.10">
        <property type="entry name" value="Ribonuclease A-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001427">
        <property type="entry name" value="RNaseA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036816">
        <property type="entry name" value="RNaseA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023412">
        <property type="entry name" value="RNaseA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437:SF65">
        <property type="entry name" value="ANGIOGENIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00074">
        <property type="entry name" value="RnaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00794">
        <property type="entry name" value="RIBONUCLEASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00092">
        <property type="entry name" value="RNAse_Pc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54076">
        <property type="entry name" value="RNase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027106074" evidence="2">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease A-domain" evidence="3">
        <location>
            <begin position="23"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013921582.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16117" checksum="FBB0F53070C7F21E" modified="2020-10-07" version="1">MWFKGFGLGLFIVLAAVLMLGTFAADYPTFLEHHFDDPKSNVGDHYCNKMMQRRGMTKPKTKEVNTFIHDTQNNIIAVCGDGGEAIDNRLRCSKKQFRVTTCKMKGSSTKPPCDYRENTSPRYIVIAWENGWRCGAASLTSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YC26</accession>
    <name>A0A6I9YC26_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vomeronasal type-2 receptor 26-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106548760</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921683.1">
        <property type="nucleotide sequence ID" value="XM_014066208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YC26"/>
    <dbReference type="GeneID" id="106548760"/>
    <dbReference type="KEGG" id="tsr:106548760"/>
    <dbReference type="OrthoDB" id="9038561at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF599">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="578"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="608"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="653"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="697"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="732"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="758"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="538"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013921683.1"/>
        </source>
    </evidence>
    <sequence length="803" mass="91736" checksum="EA1D7CB234AB83F5" modified="2020-10-07" version="1">MTQNYQHILALVFAIREINEDPQILPNLTFGFHIYDSHINTRWAFGSAMQLISPKEQFLPNYHCDRKDNLLAIIEGLDIESSYQIPNIFSIYKIPQLSYGSAPVMMDHTQSPYFYQMVPNEVYQYREILELLLYFRWTWVGFFIATGINSEWVLQVMIPEFAAHGICFAFIDTVHNVGMIIEYGDFKKWTFKLWNKFMTSTANVFLLFGKVQTPVFLDWLLVQGRDSEKTNTTKGKVWILTAQLEAKSYSNKQGQISAKLIYSGALSLVVHSNGLPGFLQFILERKPSNVKEDFLIKDFWSSAFGCTFPNWILNHTNSDLCTGLESLENISENLLEKTITGQSYSIYNAAFAVAHAFHAMYSSRFKHNRMLKRERWTSLTQQSWKLLYFLKQVSFNNTVGEKISFDQNGILLTTFDVINWVLFPNESLIGVKVGMINSQVTRDHKLMMNKNPIVWHGWFNQVQPISACNEKCQPGYSKQKKEGEPFCCYDCIPCPESKISEWEDMPDCIECKQDLYPNKHQNVCIPKIVTFLSYNEPMGMGSTTGALFLSLITIALLKTFRMHHNTPIIKANNRDLTYILLTSLLLCFLCTLLFIGQPRKETCFLQQSFFGIVFSVAVSSVLAKTITVVLAFRGTPPGTKRSKGMGKGLANSIVIFCSLIQVGICTVWLSTSPPFLDADMHSGMDEITLQCNEASPVMFYSVIGYMGFLAIASFIVAFFARNLPDSFNEAKSITFSMLVFCSVWLSFVPTYLSTKGKYMVAVEIFSILASSVGLLSFIFFPKWYIIVARPELNNKDHLKWRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YCE2</accession>
    <name>A0A6I9YCE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">Adenosine receptor A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106548844</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 15">Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013921808.1">
        <property type="nucleotide sequence ID" value="XM_014066333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YCE2"/>
    <dbReference type="GeneID" id="106548844"/>
    <dbReference type="KEGG" id="tsr:106548844"/>
    <dbReference type="OrthoDB" id="5984709at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001609">
        <property type="term" value="F:G protein-coupled adenosine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14968">
        <property type="entry name" value="7tmA_Adenosine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001068">
        <property type="entry name" value="Adeno_A1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001634">
        <property type="entry name" value="Adenosn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF1">
        <property type="entry name" value="ADENOSINE RECEPTOR A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00552">
        <property type="entry name" value="ADENOSINEA1R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00424">
        <property type="entry name" value="ADENOSINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11 15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="25"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU201114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_013921808.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="35566" checksum="A355B4DFBB98AE78" modified="2020-10-07" version="1">MSEFNPLDIVYIFIEVLIALVSIPGNILVIWAVKVNKSLRDATFYFIGSLAVADAAVGTFAVPLAITISRGPSMNFYQCLMLICPLLILTESSILALLAIAVDRYLRVKIPIRYKNVMTPRRAGVAIGCCWLISLLVGMIPLFGWNENEKDTFNSTCKFDKVISMKYMVYFNFFGCVLPPLVFMLIIYIAVFKIIRKQLSKKFGSNSACPEKYYGKELKIAKSLALVLFLFALCWLPLHICNFVALFTPELTKLQIFTPIAVCMTHLNSAMNPIIYAFRIKKFRTTFLQIWNQYFHCKMNRHSNNIGDTDIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YD30</accession>
    <name>A0A6I9YD30_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">EF-hand calcium-binding domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EFCAB5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013921940.1">
        <property type="nucleotide sequence ID" value="XM_014066465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YD30"/>
    <dbReference type="GeneID" id="106548964"/>
    <dbReference type="KEGG" id="tsr:106548964"/>
    <dbReference type="CTD" id="374786"/>
    <dbReference type="OrthoDB" id="199400at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd22968">
        <property type="entry name" value="DD_EFCAB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46788">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46788:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013921940.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29206" checksum="61C79133A02099A1" modified="2020-10-07" version="1">MAADPERENGSETDHGSDLPVGVENPSSGAALRAVTPAVDAKWKAAFHEGVQPRALHLQGIKMEKLRRKSLQQKKMEKEEPPDSLSQDWLNTESMTLETRAYLLDKLLPTLVPAVEALLKVAERKQALEPQESQPCPFDPMIFLGEYLMRHNPAYDLTAQPNPYVRGLKAITDQLMARVPDTTMHKLAGMKNLVEEKRRHREDVENIKAQVNQLREQALAVQFHEWTLDGTGQLPLALVTGANEPGPPQLAGKDLPHTLTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YDA8</accession>
    <name>A0A6I9YDA8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC106549117</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106549117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013922156.1">
        <property type="nucleotide sequence ID" value="XM_014066681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YDA8"/>
    <dbReference type="GeneID" id="106549117"/>
    <dbReference type="KEGG" id="tsr:106549117"/>
    <dbReference type="OrthoDB" id="9426071at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013922156.1"/>
        </source>
    </evidence>
    <sequence length="307" mass="33404" checksum="B59592EAFCD6CE61" modified="2020-10-07" version="1">MVPIKPNDGSPGLYNTVRDKDALPSDAKGNPPSSSLAKALDAVITAGRRGLLGPAAMASPVPALNVGGCNGVASGGNLNRIPLVAGCDQRKRAVNQKRTSVITVVPSAEGVGNLPPKPKEIWICGNSVILASKKRAQSHPQGLQLGISPSRANVFWHGIEDMMWTQLMPALHEIYHSRSPPSVIIIHLGEDDLFPENNTSVIISMKNDLGILKRAFPDAVIVWSSLLPRRIWKYDPKPRYTETAQILVNNIMVTYCNKIGVHFLSHNSITSDKSDLFLPDVSNLTDEGVDIFIADLKKVLRLYQILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE07</accession>
    <name>A0A6I9YE07_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Golgi phosphoprotein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GOLPH3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922274.1">
        <property type="nucleotide sequence ID" value="XM_014066799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE07"/>
    <dbReference type="GeneID" id="106549212"/>
    <dbReference type="KEGG" id="tsr:106549212"/>
    <dbReference type="CTD" id="64083"/>
    <dbReference type="OrthoDB" id="2189106at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000003">
        <property type="entry name" value="Golgi phosphoprotein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF3">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013922274.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="28919" checksum="8CBAB0159FE6EFA8" modified="2020-10-07" version="1">MTSLTQRSSGLVQRRTEASRSAADKDRGAGGGNGGGGGGGGPEEEGRRDEQGEEEKGDSKETRLTLMEEVLLLGLKDREVICKSDAPTGDVLLDEALKHIKETQPPETVQNWIELLSGETWNPLKLHYQLRNVRERLAKNLVEKGVLTTEKQNFLLFDMTTHPLTNNNIKQRLIKKVQEAVLDRWVNDPHRMDKRLLALIYLAHASDVLENAFAPLLDEQYDLATKRVRQLLDLDPEVECTKANTNEVLWAVVAAFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YE88</accession>
    <name>A0A6I9YE88_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable ATP-dependent RNA helicase DDX5</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">DEAD box protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">DDX5</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="33">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Identified in the spliceosome C complex. Component of a ribonucleoprotein complex containing mRNAs and RNA-binding proteins including DDX5, HNRNPH2 and SRSF1 as well as splicing regulator ARVCF. Interacts with RBM4; the interaction occurs in an RNA-independent manner. Interacts with AGO1 and AGO2. Interacts with ESR1, AR, EP300, CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interacts with DDX17. Interacts with BRDT. The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1 and POLR2A (active). Interacts with DHX36; this interaction occurs in a RNA-dependent manner. Interacts with NUPR1. Interacts with ERCC6. Interacts with DDX3X in the cytoplasm; this interaction may be more efficient when both proteins are unphosphorylated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="RefSeq" id="XP_013922488.1">
        <property type="nucleotide sequence ID" value="XM_014067013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YE88"/>
    <dbReference type="GeneID" id="106549382"/>
    <dbReference type="KEGG" id="tsr:106549382"/>
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    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18049">
        <property type="entry name" value="DEADc_DDX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012587">
        <property type="entry name" value="P68_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08061">
        <property type="entry name" value="P68HR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01414">
        <property type="entry name" value="P68HR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 27 33" id="KW-0347">Helicase</keyword>
    <keyword evidence="12 27" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="21" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="20" id="KW-0804">Transcription</keyword>
    <keyword evidence="18" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="31">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="29">
        <location>
            <begin position="121"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="30">
        <location>
            <begin position="324"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="26">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="28">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="474"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00032351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00046488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_013922488.1"/>
        </source>
    </evidence>
    <sequence length="600" mass="67262" checksum="979EF9CEE2A96580" modified="2020-10-07" version="1">MPGYSSERDRGFGGPRFGGSRGGSLGGKKFGNPGEKLTKKKWNIDELPKFEKNFYQEHPDVARRAMQEIEQYRASKEITVKGHNCPKPVMNFYEANFPANVMEVIQRQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYGRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLEAGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVEQIRPDRQTLMWSATWPKEVRQLAEDFLKEYVHINIGALELSANHNILQIVDVCHDVEKDDKLIRLMEEIMSEKENKTIVFVETKRRCDDLTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLIEDRGSGRSRGDRRDRYSAGKRGGFGFRERENFERTYGAVGKRDFGAKAQNGGFGAQSFGNGTTFGSSFGAAGMQGGFRAGTSAYQNGYDQQYGNNMANMHSAMNQQQYAYPTTGGTPMIGYPMPANYTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YF31</accession>
    <name>A0A6I9YF31_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">NADPH--cytochrome P450 reductase</fullName>
            <shortName evidence="10">CPR</shortName>
            <shortName evidence="10">P450R</shortName>
            <ecNumber evidence="10 11">1.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 15" type="primary">POR</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="10">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="10">Binds 1 FMN per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Single-pass membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NADPH--cytochrome P450 reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.4" evidence="10 11"/>
    <dbReference type="RefSeq" id="XP_013922803.1">
        <property type="nucleotide sequence ID" value="XM_014067328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YF31"/>
    <dbReference type="GeneID" id="106549626"/>
    <dbReference type="KEGG" id="tsr:106549626"/>
    <dbReference type="CTD" id="5447"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06204">
        <property type="entry name" value="CYPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000001">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000009">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000001">
        <property type="entry name" value="NADPH--cytochrome P450 reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03212">
        <property type="entry name" value="NCPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023208">
        <property type="entry name" value="P450R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000208">
        <property type="entry name" value="P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0274">FAD</keyword>
    <keyword evidence="1 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 11" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="12">
        <location>
            <begin position="84"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="13">
        <location>
            <begin position="283"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="90"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="458"/>
            <end position="461"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="476"/>
            <end position="478"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="492"/>
            <end position="495"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="538"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="599"/>
            <end position="600"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="605"/>
            <end position="609"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="642"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="680"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_013922803.1"/>
        </source>
    </evidence>
    <sequence length="680" mass="77146" checksum="BC52EE6E73FD4BD5" modified="2020-10-07" version="1">MGTAASKDEPSSAQSPEASTAFSMMDIFLFSIVIGLLTYWFFFRKKKEEIPEFSKIQTPGAGTTEQSANENSFIEKMKKTGRNIVVFYGSQTGTAEEFANRLAKDAQRYGMRGMSADPEEYDLSDLSHLAEIDKSLAVFCMATYGEGDPTDNAQDFYDWLQDTDSKLSGIKYAVFGLGNKTYEHFNAMGKFVDKKLEELGAQRIYELGMGDDDGNLEEDFITWREQFWPAVCEHFGVEATGDESSIRQYELVVHKDISPSKVYKGEMGRLKSYENQKPPFDAKNPFLAPVSLNRKLNQGGERHLMHHELDITGSKIRYESGDHIAVYPANDTSLVNQLGEILGADLDTVISLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQYATNASEQEHLRKMASSSAEGKTLYLSWVVESRRNILAILQDTPSLRPPIDHLCELLPRLQARYYSIASTSKVNPNSIHICSVVVEYTTKTDRVNKGVATNWLKNKQPNENGHKSTVPLYVRKSQFRLPFKPNTPIIMIGPGTGIAPFIGFIQERGWLKQQGKEVGETVLYFGCRHEKEDYIYKDELAKFVKEGALTQLNVAFSRDQPEKIYVQHLLKKNKEHVWKLISEGNGHIYVCGDARNMARDVQNAFYEIVAEYGKMDHQQAVDYVKKLMTKGRYSLDVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YG15</accession>
    <name>A0A6I9YG15_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Group 10 secretory phospholipase A2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106549676</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013922855.1">
        <property type="nucleotide sequence ID" value="XM_014067380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YG15"/>
    <dbReference type="GeneID" id="106549676"/>
    <dbReference type="KEGG" id="tsr:106549676"/>
    <dbReference type="OrthoDB" id="10069378at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000001">
        <property type="entry name" value="Basic phospholipase A2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF4">
        <property type="entry name" value="GROUP 10 SECRETORY PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027166545" evidence="8">
        <location>
            <begin position="21"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="53"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="70"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013922855.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17921" checksum="C5056648AC93C478" modified="2020-10-07" version="1">MVRQLVTLLLFHAVWNGVLGKNHFVTKRGLIELYGALKCGTSRFSLAYVGYGCYCGLGGRGWPKDATDWCCHRHDCCYDFAQRQGCNPITERYKWTCQDNTVICDAVLDRCQSIICQCDREAARCWRFAPFNKRYIFWPNFLCGQIYPSCSYWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGL5</accession>
    <name>A0A6I9YGL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA-binding protein 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">RNA-binding motif protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106550143</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923433.1">
        <property type="nucleotide sequence ID" value="XM_014067958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGL5"/>
    <dbReference type="GeneID" id="106550143"/>
    <dbReference type="KEGG" id="tsr:106550143"/>
    <dbReference type="OrthoDB" id="79941at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098534">
        <property type="term" value="P:centriole assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12343">
        <property type="entry name" value="RRM1_2_CoAA_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000046">
        <property type="entry name" value="RNA-binding protein 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF26">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN M-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="78"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013923433.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="35102" checksum="269FD6DEA9E9B62A" modified="2020-10-07" version="1">MVKIFVGGLSPVVTAEELRKLFEQYGQVNECDILKNFAFVHMEKEDEAHQAISTLHKSEFYGTHLTVEYATSKIRNATKIYVGNVSAKATTAQIRQLFERFGKVVECDIVKNFAFVHMAKEREAMDAILHLNDTPLEDQKIFVTLSKSNSSLRATKGASVPTPATPTSSYYFSRGRVPTAPATYSPYCARGWYDREYCDQYTYELYDRARAAYDRALPSAPAAYRERSPVGRRTSTTPTAVVAQYTDPYAAVAVAAAAAAAMAAANQTYSQAYSQPGYAAASAAATYSQYSLGTAYNVAEYYERYASAYADQYAQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YGN9</accession>
    <name>A0A6I9YGN9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spermatid perinuclear RNA-binding protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106549835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013923060.1">
        <property type="nucleotide sequence ID" value="XM_014067585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YGN9"/>
    <dbReference type="GeneID" id="106549835"/>
    <dbReference type="KEGG" id="tsr:106549835"/>
    <dbReference type="OrthoDB" id="8898434at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19911">
        <property type="entry name" value="DSRM_STRBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000006">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF62">
        <property type="entry name" value="AT07585P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="91"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923060.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="27190" checksum="E4B965961A8AC21D" modified="2020-10-07" version="1">MSVDVDGTTYEASGPSKKTAKLHVAVKVLQAMGYPTGFDADMECTSSDEKSDTEGKNETVSSTTSNNNGNSTVDTSATLEVRTQGPILTASGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGQRFRGAGPNKKVAKASAALAALEKLFSGPNAAANKKKKILPQTKGGIVNTAMSAAARGRGRGSPMRGAFVGAAAATSYLAPGYAAPYGYNAAAAAAPAYGGCFIDSPYCQPLDVTPFIIHLGPQEFFSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YIX1</accession>
    <name>A0A6I9YIX1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Scaffold attachment factor B1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106550497</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013923899.1">
        <property type="nucleotide sequence ID" value="XM_014068424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YIX1"/>
    <dbReference type="GeneID" id="106550497"/>
    <dbReference type="KEGG" id="tsr:106550497"/>
    <dbReference type="OrthoDB" id="9049301at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051738">
        <property type="entry name" value="SAF_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15683">
        <property type="entry name" value="SCAFFOLD ATTACHMENT FACTOR B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15683:SF6">
        <property type="entry name" value="SCAFFOLD ATTACHMENT FACTOR B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="153"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="305"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013923899.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="38306" checksum="0673DA09F8DE231A" modified="2020-10-07" version="1">MVSEITPKKAPKRSSKGRRLEEEGVEDNGLEDESGDGQEDIEASLDNLQDIDMMDISVLDEAVIDNSSGVDCREDSSADSRLDSLFDSKESADAERRGLPEQLTGGALANMEAAALSLSDMPEESREIPVAMGKAEEASSIIDLTSDDLATLKEPDEFPLESENEKVLDILGDTCKSEPLKEDTSEAEQEQETNNSWPGKQEAVEEEQEEEEEEEKEEEGEEEDMLAATSPAHSDEDLVDKESQSGKPAEAKNQEDDAKLLVVAKGEPSKQTVEEDKSDSDSLAVESRSGGGGGDLKATFISTKSQDRKSESKEKQDILSFDKIKEQRERERQRQREREIRETERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJC6</accession>
    <name>A0A6I9YJC6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RING finger and SPRY domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RSPRY1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013924074.1">
        <property type="nucleotide sequence ID" value="XM_014068599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJC6"/>
    <dbReference type="GeneID" id="106550656"/>
    <dbReference type="KEGG" id="tsr:106550656"/>
    <dbReference type="CTD" id="89970"/>
    <dbReference type="OrthoDB" id="10017393at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16566">
        <property type="entry name" value="RING-HC_RSPRY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12883">
        <property type="entry name" value="SPRY_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045129">
        <property type="entry name" value="RNF123/RKP/RSPRY1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035774">
        <property type="entry name" value="SPRY_RSPRY1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13363:SF6">
        <property type="entry name" value="RING FINGER AND SPRY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13363">
        <property type="entry name" value="RING FINGER AND SRY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="299"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="526"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="53"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013924074.1"/>
        </source>
    </evidence>
    <sequence length="575" mass="64830" checksum="0F05940EA3FA28D1" modified="2020-10-07" version="1">MIVVALVVLYASRNLLQGLRLTFERHILCLLQTLGVSSMGNSCVCREDSSAEENAESRNHQAENSRMPVPETRSHQRDPVRPPRRGRGPHEPRRKKHNVDVLVLDTLAVIRTLVDNDQEPPYSMMTLHEMAETDEGWLEVVQSLIRVIPLEDPLGPAVITLLLDECPLPTKDALQKLTEILNLNGAVALQDACHPAKHRNTTAVLGCLAEKLAGPASVGLLSQGILEYLLQSLEFQSHPTVMLFALIALEKFAQTSENKMTVSDTCISDQLVLLEKWTNNPDYLKRQVGFCAQWSLDNLFLKEGRQFTYEKVDLTNIRAMLNSNDVSEYLKISPHGLEARCDASSFESVRCTFCVDSGAWYYEVTVITSGVMQIGWATKDSKFLNHEGYGIGDDEYSCAYDGCRQLIWYNARSKPHSHPCWKEGDTIGFLLDLQEKKMVFYLNGNQLPAEKQVFSSAISGFFAAASFMSYQQCEFNFGARPFKYPPSAKFNTFNDYAFLTAEEKIILPRHRRLALLKQVSIRENCCTLCCDEIADTELRPCGHSDLCMECALQLETCPLCRQEIQTRVRQIAHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YJW4</accession>
    <name>A0A6I9YJW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RPL19</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924663.1">
        <property type="nucleotide sequence ID" value="XM_014069188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YJW4"/>
    <dbReference type="GeneID" id="106551159"/>
    <dbReference type="KEGG" id="tsr:106551159"/>
    <dbReference type="CTD" id="6143"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="8">
        <location>
            <begin position="12"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924663.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="24443" checksum="2E89C037738F9B1F" modified="2020-10-07" version="1">MFAMMGLKDLGMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVHSRARCRKNTLARRKGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDRHMYHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREERLQAKKEEIIKTLSKEEETKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKA7</accession>
    <name>A0A6I9YKA7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dehydrogenase/reductase SDR family member 13-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106551004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924474.1">
        <property type="nucleotide sequence ID" value="XM_014068999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKA7"/>
    <dbReference type="GeneID" id="106551004"/>
    <dbReference type="KEGG" id="tsr:106551004"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF44">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="3">
        <location>
            <begin position="40"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013924474.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="34666" checksum="F0979D9B28066C14" modified="2020-10-07" version="1">MEAAGALGAGVALLLGLYALLYYNFVAATRCRNTVSLRGKTVLITGGNTGIGKATAVDLARRGARVILACRDKARGESAMYDIRRESGNSEVILMILDLCSLDSVRAFAQTFLESEPRLDILINNAGVYKGGPTADGFDQAFQVNHLAHFLLTHLLLDRLKRCAPSRIVILSSCVHTFGKIDFQSTNKPNKWLMKGGQSYNNSKLANILHARELANRLEGTNVTCYAVHPGVVKTEITRSHPWWTLWLVWSSKLFVRNSKAGAQTSIYCATEEGIEGLSGRYFADCRPKVPSPLACGDQLAKKLWEFSERLLGLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YKT5</accession>
    <name>A0A6I9YKT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RPLP0</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924983.1">
        <property type="nucleotide sequence ID" value="XM_014069508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YKT5"/>
    <dbReference type="GeneID" id="106551401"/>
    <dbReference type="KEGG" id="tsr:106551401"/>
    <dbReference type="CTD" id="6175"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="111"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013924983.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="34288" checksum="C70E0BFA3FD94295" modified="2020-10-07" version="1">MPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLSHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCDVLVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLIIQQVFDNGSIYNPEVLDITEETLHKRFLEGVRNVASVCLQIGYPTVASLPHSIINGYKRVLAVAVETNYSFPLAEKVKAFLADPSAFAAAVPVVTETAAPAAAAAPKEEVKEESEESDEDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLE7</accession>
    <name>A0A6I9YLE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 501-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106551100</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013924590.1">
        <property type="nucleotide sequence ID" value="XM_014069115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLE7"/>
    <dbReference type="GeneID" id="106551100"/>
    <dbReference type="KEGG" id="tsr:106551100"/>
    <dbReference type="OrthoDB" id="9007773at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003000">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001530">
        <property type="entry name" value="Zinc finger protein 268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000224">
        <property type="entry name" value="Zinc finger protein 329"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000951">
        <property type="entry name" value="Zinc finger protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF15">
        <property type="entry name" value="REDUCED EXPRESSION 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013924590.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="43584" checksum="8B04E2E640542780" modified="2020-10-07" version="1">MMAGRENIQDLKEPVLNPNKPTEEQPEEPETDPVAMKRRDLYTTILRQPIPKGKQPSDKLFHSLNKHLQAHTGKELFECSDCGKAFFSRAGFIVHIRVHGQKKPYECSECGKHFRRSSHLNNHSKIHAGVKPFQCSDCGKTFSRSSSLVSHQHMHTGEKPYHCASCSKEFCDKYSLMRHERLHTGDKPYKCTECGKSFNQSHHLITHKRSHTGEKPYVCLLCSKSFCDRSTYIRHQKNHMGEKMFKCSECGESFSRNKHLLRHQRIHTTGELYTCSDCGENFSRRSGLKMHQKIHTGEKPYECGDCGKKFNRSTNLISHQRVHTGERPYGCPDCEKRFSRRSHLVSHQRIHGQKKEMPQGLLHPPAGVYMPTLFITTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLS8</accession>
    <name>A0A6I9YLS8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cleavage stimulation factor subunit 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106551713</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925333.1">
        <property type="nucleotide sequence ID" value="XM_014069858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLS8"/>
    <dbReference type="GeneID" id="106551713"/>
    <dbReference type="KEGG" id="tsr:106551713"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12671">
        <property type="entry name" value="RRM_CSTF2_CSTF2T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.70:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.630:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000061">
        <property type="entry name" value="cleavage stimulation factor subunit 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="16"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="199"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="294"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="385"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013925333.1"/>
        </source>
    </evidence>
    <sequence length="647" mass="69566" checksum="EDE17B0102BDE1E0" modified="2020-10-07" version="1">MAGLSVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPIIESPYGDPVNPEDAPESISRAVASLPPEQMFELMKQMKLCVQNSPQEARSMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTSVPSLIPGNAQPGPGQGPGPGPGAGPGPGPGPGPGPNAQMNQPNVPSSQPQPIFASQALLFSLVHPPDCFSQCSPAGSMPGSGPMILERGQVPMPDPRAPMHRGPMPASGPPPRGLLGDAPNDPRGGTLLSVTGDVEPRGYLGPPHQGPPMHHMPAHDSRGLPPHEMRGGPMGEPRPLMGEPRGPLMDGRGGRDPRGMEPRVMDGRGMEPRVMDGRGMEPRGMEPRGMESRGMEPRVMDGRGMEPRGMEPRGMEPRGMEPRGMDARGMEPRGMEPRGMESRGMEPRVMDGRGMEPRGMEPRGMEPRGMEPRGMDARGMEPRGMASRGMDARGIEPRGMEPPRGMEPRGMEPPRGMEPPRGMEPPRGMEQVPNMPGTTMQPSGAMAGGAVQPAAQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YLV7</accession>
    <name>A0A6I9YLV7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="15">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">GALT</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays an important role in galactose metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="14">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="15"/>
    <dbReference type="RefSeq" id="XP_013924770.1">
        <property type="nucleotide sequence ID" value="XM_014069295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YLV7"/>
    <dbReference type="GeneID" id="106551237"/>
    <dbReference type="KEGG" id="tsr:106551237"/>
    <dbReference type="CTD" id="2592"/>
    <dbReference type="OrthoDB" id="418412at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000001">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000002">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008724">
        <property type="entry name" value="PRK11720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943:SF1">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 15" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="17">
        <location>
            <begin position="6"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase C-terminal" evidence="18">
        <location>
            <begin position="186"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="12">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="31"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="80"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="162"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="317"/>
            <end position="318"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="322"/>
            <end position="323"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_013924770.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="41522" checksum="187FEFF3CFAFFDB6" modified="2020-10-07" version="1">MEAAAKFQPSEHQHLRYNPLREDWVLVSAHRMRRPWQGQVEKPPEEEPPRHDPANPLCPGATRANSKVNPHYESTFVFDNDFPALQPDAPEPEPDEHPLLRSASARGVCKVMCFHPWTDLTLPLMSLAEIRRVIDKWVEIAVELGASYTWVQIFENKGAMMGCSNSHPHCQIWASNFLPNEASLEDKSQRKYYQDNAAPMLLDYARLEAERKERVVVENADWLVVVPYWAVWPFQTLLLPRRHVCRLEELREGERDSLASIMKRLLTRYDNLFQVSFPYSMGWHGAPTGPYLEADCRHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRKLPEHHYKLEAKEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YNA1</accession>
    <name>A0A6I9YNA1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC106551619</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_013925235.1">
        <property type="nucleotide sequence ID" value="XM_014069760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YNA1"/>
    <dbReference type="GeneID" id="106551619"/>
    <dbReference type="KEGG" id="tsr:106551619"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14165">
        <property type="entry name" value="STKc_TSSK1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000658">
        <property type="entry name" value="Protein CBG12184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF102">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="12"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="299"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_013925235.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="38686" checksum="1BA98C942FD8037E" modified="2020-10-07" version="1">MDDGEVLKKRGYIMGINLGEGSYAKVKSAYSERLKCNVAVKIIDKKKAPRDFLDRFLPREIEMLARVKHYAIVKTYEIFETSEGKVYIVTELGVQGDLLEFIKKKGAIPEEIARKMFNQLASAMKYCHELDIVHRDLKCENLLLDKEYNIKLTDFGFAKRLSRDDDGRVILSKTFCGSAAYAAPEVLQGIPYQPKVYDIWSMGVVLFIMVCGSMPYDESNIKKMLKLQKEHRVHFPRSKILSVECKDLIYHMLQPDVTRRLCIDEVLMHIWMQEPKAVSDSMLTKPGECSQSQIRSLFGKESISQPSDNEQVKQEIRHEDLENLDTVSDQMQRTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YPA2</accession>
    <name>A0A6I9YPA2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">UBX domain-containing protein 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">UBXN2A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts to repress the ubiquitination and subsequent endoplasmic reticulum-associated degradation of CHRNA3 by the STUB1-VCP-UBXN2A complex in cortical neurons. Also acts to promote the translocation of CHRNA3 to the plasma membrane and subsequently increases plasma membrane acetylcholine-gated ion-channel activation. Plays a role in the inhibition of STUB1-mediated TP53 degradation, via its interaction with HSPA9 which acts to inhibit TP53 binding to HSPA9. Positively mediates the ubiquitination and proteosomal degradation of RICTOR, may thereby act as a negative regulator of the mTORC2 pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Part of a complex composed of STUB1/CHIP, VCP/p97, CHRNA3, and UBXN2A that modulates the ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of CHRNA3. Within the complex UBXN2A acts as a scaffold protein required for the interaction of CHRNA3 with VCP/p97, this interaction also inhibits CHRNA3 ubiquitination by STUB1/CHIP and subsequently ERAD. Interacts (via SEP domain) with CHRNA3 and interacts (via UBX domain) with VCP/P97; these interactions are required for the interaction of CHRNA3 with the STUB1-VCP-UBXN2A complex. Interacts with HSPA9/MOT-2 (via SBD domain); the interaction inhibits HSPA9/MOT-2 interaction with and degradation of p53, thereby promotes p53 translocation to the nucleus. Interacts with RICTOR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013925734.1">
        <property type="nucleotide sequence ID" value="XM_014070259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YPA2"/>
    <dbReference type="GeneID" id="106552068"/>
    <dbReference type="KEGG" id="tsr:106552068"/>
    <dbReference type="CTD" id="165324"/>
    <dbReference type="OrthoDB" id="25887at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000164">
        <property type="entry name" value="UBX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000004">
        <property type="entry name" value="UBX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF16">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SEP" evidence="17">
        <location>
            <begin position="56"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="16">
        <location>
            <begin position="166"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_013925734.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="29208" checksum="53B6F74F4E674618" modified="2020-10-07" version="1">MQELDKIEILNPEWICQLKTDGQIQNGTKQNNSDGCVDKLFEEAQKVGAICSEQATADTIITLWKNGFTINDGELRSYTDVANRRFLDSIKKGELPHELQKICDKEEVDVKVDDKKDELYTSPKAVFHPFSGAGYRLGSATPRIISKVKKKGEETEKRKPTVVLNNSEPITRIQIWLADGARIVQNFNISHRVSHIRDFIMDYQRHQGKRPFTLTTSLPFQELLDESLTLEEANLKNAVVVQRLKNNTEPFRSFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YQ93</accession>
    <name>A0A6I9YQ93_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Forkhead box protein E1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FOXE1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013926683.1">
        <property type="nucleotide sequence ID" value="XM_014071208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YQ93"/>
    <dbReference type="GeneID" id="106552831"/>
    <dbReference type="KEGG" id="tsr:106552831"/>
    <dbReference type="CTD" id="2304"/>
    <dbReference type="OrthoDB" id="5402974at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20019">
        <property type="entry name" value="FH_FOXE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000201">
        <property type="entry name" value="Forkhead box E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF156">
        <property type="entry name" value="FORKHEAD BOX PROTEIN E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="69"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="69"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013926683.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="38740" checksum="2520BD9E8443FCAF" modified="2020-10-07" version="1">MTAESPQPTGCAVSRPDAEAEAASRLPALVKMEPAAPGEALEPEPSEEDGMGSSRPIGRRRKRPVQRGKPPYSYIALIAMAIAHSPERRLTLGGIYRFITERFPFYRDGPRKWQNSIRHNLTLNDCFVKVPREPGRPGKGSYWALDPHARDMFESGSFLRRRKRFKRSDLSTYPAYMHEAPAQPVGPSLSSAYAPPAALCYPSQPPLFSLGPLMAQPSPELVQQHSMDINSSPSNACSFAGSAAYSNQACTAASGLGRPHNTMPYSYAIPSAQQLQVNQGAYSQSSSSNLFGASARLGVPTSPPITNDSMDFYGRMSPSSYSSLAHGYNAGGQLSSPGAYLRHTSYPSNMERFVSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YRJ8</accession>
    <name>A0A6I9YRJ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heterogeneous nuclear ribonucleoprotein C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106552881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013926751.1">
        <property type="nucleotide sequence ID" value="XM_014071276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YRJ8"/>
    <dbReference type="GeneID" id="106552881"/>
    <dbReference type="KEGG" id="tsr:106552881"/>
    <dbReference type="OrthoDB" id="9042265at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF26">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013926751.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28848" checksum="C1E04C9976BC6EDF" modified="2020-10-07" version="1">MKKGVGVGKEKTAAHQPRWGLGERERLTDSGTWHWVFLSSPPPSGMTLFGRREGCWRYINMAQEPKLSHTRPGQKRQHTASLYPNNCDLEYELYKDDLPYRIYEYQKIPSLIGHIPIKTRRSHLGAESKTRLNPHTDLRSNSNPTTGRTKLRAEELHSIKGELSQIKAQVDNLLESLNRMDQQREHPTGTKDSKRKLFTSVEASSPAGELIRMKESAKNEGHAPQQEIDSAEESTDTEEATAARNHTPDPEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSH4</accession>
    <name>A0A6I9YSH4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553288</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="RefSeq" id="XP_013927237.1">
        <property type="nucleotide sequence ID" value="XM_014071762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSH4"/>
    <dbReference type="GeneID" id="106553288"/>
    <dbReference type="KEGG" id="tsr:106553288"/>
    <dbReference type="OrthoDB" id="28397at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000415">
        <property type="entry name" value="CMGC/CDK/CRK7 protein kinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF459">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="310"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="456"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="659"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="474"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="669"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="682"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927237.1"/>
        </source>
    </evidence>
    <sequence length="738" mass="81970" checksum="6ED96BD48DFB1F64" modified="2020-10-07" version="1">HKKNFLHRDIKCSNILLNNRGQIKLADFGLARLYSSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANQELAQLELISRICGSPCPAVWPDVIKLAYFNTMKPKKQYRRRLREEFAFIPAAALDLFDYMLALDPSKRCTAEQALQCEFLRDVDPSKMPPPDLPLWQDCHELWSKKRRRQKQMGMTDDAAKVPRKDLSLGMNDSRTNTPQGLQTSSQLKMQSNSNIALVKAGSGQLLNQNEVAILLNLLQSKSSINMAQFAQVLNIKMNPETEQQLNKINLPAGILDVAEKQSEQQQQQQQQQQQQQQQQQPPPPPKPPLPPSPDKEPLKQTATPQLSVQATPLTQSKINTEVTQAAVQSAFTVLLSQLLKTQQLKQKENFLEEKENGSGNDMSLQSRQPPEPSTPVSANWEDVESPASVYPHLIKSLYTCAGEDDLVRPPEMKPQTPEERPRILPPDQRPPEPPEPPPVTDEDLDYRTANQHLPSISSSSGMDPHAGVKAALLELLAHHQAQTSEKPATSNVEFQSRDSFVAGPEYKDNFASTTFSCAHYSGGDGIGNISSGTLERANFVGNSDVQPLEIYNTASSHSSSALHPPAFSEPFHSSVTRYGDIYLNSGSMLFSGDKDHRFEYSHGPITVLENSGDSVGRSGSESTHSHSLSSKMQNYSYGSNLQEPPASVSHIHGQTWTSPAQGPGYSQGYRGQISTSAIRGRGRGLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YSN7</accession>
    <name>A0A6I9YSN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">ITGB2</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 23">Cell membrane</location>
            <topology evidence="1 23">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Membrane raft</location>
            <topology evidence="21">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 23">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013926954.1">
        <property type="nucleotide sequence ID" value="XM_014071479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YSN7"/>
    <dbReference type="GeneID" id="106553055"/>
    <dbReference type="KEGG" id="tsr:106553055"/>
    <dbReference type="CTD" id="3689"/>
    <dbReference type="OrthoDB" id="410592at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007159">
        <property type="term" value="P:leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="FunFam" id="1.20.5.100:FF:000008">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000076">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000098">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.1680.10:FF:000012">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033760">
        <property type="entry name" value="Integrin_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF15">
        <property type="entry name" value="INTEGRIN BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07965">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17205">
        <property type="entry name" value="PSI_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002512">
        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 23" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 23 31" id="KW-0401">Integrin</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 25" id="KW-0732">Signal</keyword>
    <keyword evidence="6 23 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026916425" description="Integrin beta" evidence="25">
        <location>
            <begin position="32"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="27">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="26">
        <location>
            <begin position="41"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="29">
        <location>
            <begin position="631"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="28">
        <location>
            <begin position="734"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="34"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="45"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="200"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="255"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="429"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="454"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="476"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="492"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="521"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
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            <begin position="523"/>
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    <feature type="disulfide bond" evidence="22">
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            <begin position="528"/>
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    <feature type="disulfide bond" evidence="22">
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            <begin position="545"/>
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        </location>
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    <feature type="disulfide bond" evidence="22">
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    <feature type="disulfide bond" evidence="22">
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    <feature type="disulfide bond" evidence="22">
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        </location>
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    <feature type="disulfide bond" evidence="22">
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            <begin position="584"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
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    <feature type="disulfide bond" evidence="22">
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    <feature type="disulfide bond" evidence="22">
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    <feature type="disulfide bond" evidence="22">
        <location>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000504617"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="780" mass="86870" checksum="A0CF98805B14E55B" modified="2020-10-07" version="1">MKPQRLRDEMFPKYFLLLFGALLLLFTTAFSLRCTKYNVKTCQDCIESGPGCAWCKKRNFTKAGEPDSVRCDTKQQLLEKGCEGNIIFPENFIDPVSSAQSNTKKQIYPEVVRLHLRPEQPAVFNVTFQRGEDYPIDLYYLMDLSFSMADDLQTVKKLGGDLLKALQGITKRARIGFGAFVDKTVLPFVNTHPKKLQNPCPTTEIKCQPPFAFRHVLSLTDDVQSFQEEVGKQNISGNLDAPEGGLDAMMQAAVCENIIGWKNVTRLLVYTTDDGFHFAGDGKLGAILTPFDGQCHLEDNMYKKSNEYDYPSVGQLIQKLKEKKIQPIFAVTRRVYDTYEKLSKMIPKSAVGQLQEDSNNIVQLIQGAYNDLSSKIILEHSSVPSSIKISYDSFCLNQIHTKNQERGECDNVKINDKITFQVQITATSCIKNQTFTLQPLGFTDLTTVHIYSRCNCECDEELPNESDCNGQGNINCGICRCNAGYVGKNCDCKTGGKTSKELEKSCRKDNSSVICSGLGDCVCGQCICHTMADTQKHIYGPFCECDNINCELHNGQVCGGKDRGSCDCGKCKCTAQYDGTACQCQSSTEKCKNKNGNVCSLRGQCQCNTCKCNGRYQPPLCEECPSCPSPCARYISCVECLAFGSGPFQKNCSDSCSNITLESEAQPKSIHCRDKDSENCWISYTMLPTDGEEHYSITVNREKECPQPPNIAAIVGGTIAGVALIGLLILLAWRLVAELFDRREYHRFEKEKSKAKWNEADNPLFKSATTTVVNPRFNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YST1</accession>
    <name>A0A6I9YST1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pleiotropic regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PLRG1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in pre-mRNA splicing as component of the spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its WD40 repeat domain) directly with CDC5L (via its C-terminal); the interaction is required for mRNA splicing but not for spliceosome assembly. Component of the minor spliceosome, which splices U12-type introns. Within this complex, interacts with CRIPT. Also interacts directly in the complex with BCAS2 and PRPF19. Interacts with USB1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PRL1/PRL2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927608.1">
        <property type="nucleotide sequence ID" value="XM_014072133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YST1"/>
    <dbReference type="GeneID" id="106553595"/>
    <dbReference type="KEGG" id="tsr:106553595"/>
    <dbReference type="CTD" id="5356"/>
    <dbReference type="OrthoDB" id="10256122at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000012">
        <property type="entry name" value="Putative pleiotropic regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="4"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="46"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="88"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="213"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_013927608.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="35336" checksum="5E6621880BAE0E00" modified="2020-10-07" version="1">LQVISGHLGWVRCIAVEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSARSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVKCQAAEPQIITGSHDTTIRLWDMVAGKTRVTLTNHKKSVRAVVLHPRQYTFASGSPDNIKQWKFPDGNFIQNLSGHSAIINTLAVNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFSCAFDQSESRLLTAEADKTIKVYKEDDSATEETHPVSWKPEIMKRKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTF3</accession>
    <name>A0A6I9YTF3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Afadin- and alpha-actinin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SSX2IP</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927828.1">
        <property type="nucleotide sequence ID" value="XM_014072353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTF3"/>
    <dbReference type="GeneID" id="106553784"/>
    <dbReference type="KEGG" id="tsr:106553784"/>
    <dbReference type="CTD" id="117178"/>
    <dbReference type="OrthoDB" id="312015at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052300">
        <property type="entry name" value="Adhesion_Centrosome_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021622">
        <property type="entry name" value="Afadin/alpha-actinin-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46507">
        <property type="entry name" value="AFADIN- AND ALPHA-ACTININ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46507:SF1">
        <property type="entry name" value="AFADIN- AND ALPHA-ACTININ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11559">
        <property type="entry name" value="ADIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="126"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="241"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013927828.1"/>
        </source>
    </evidence>
    <sequence length="565" mass="66054" checksum="63FE99C752F21E74" modified="2020-10-07" version="1">MGDWTSVTVPELSSDNNITWYTSSRNMSPSSLSVPQVQNSALPPSKNMHSFFSVLCTEENIEQSLSYLNKELTTLGFPSLYADSRGKEVSIVSVLNCMNELLLMQRKTLRAQEETETQHLKMGSDMDHLQNCYIKLKEQLESSRREIVGLQERDRQLQCKNRNLHQLLKNEKDEIQKLQNIVASRASQYNHDMKRKEREYNKLKERLHQLLMSKKDKKIAIDILNYVGRIDGKRGAWRTGKTEAKNEEEMFKTLLNEYEQRQKQLLVENAELKKLLQQMKKEIISLLPSQKQMPKEKIEDNTGPILSDLEEDSGEANKEDMWELSCETVREQLANSIRKQWRMLKNHVENLDNQGDVYFPYNIYFPYLTFYLQQQLTAPCDDDTNMLLQDCYLLEEKERLKEEWKLFKEQKKNFEKERKSFTDAAIRLGLERKAFEEDRGVWLKQQFLNMTADQNKADRMKAQNAFLGSSESDNTLTNVRSHQKKLNHMSNVPKFSDVYQSTQHIIENSLSTFLDKIPEDQKTNQMKREPLMLQIKETSPVAEDSGNNIHVPLSDLCTDHIINSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTT5</accession>
    <name>A0A6I9YTT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein AMN1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">AMN1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with TASOR.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AMN1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927978.1">
        <property type="nucleotide sequence ID" value="XM_014072503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTT5"/>
    <dbReference type="GeneID" id="106553918"/>
    <dbReference type="KEGG" id="tsr:106553918"/>
    <dbReference type="CTD" id="196394"/>
    <dbReference type="OrthoDB" id="10257471at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000178">
        <property type="entry name" value="protein AMN1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF254">
        <property type="entry name" value="PROTEIN AMN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="4">
        <location>
            <begin position="104"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_013927978.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="28771" checksum="7A8BB033B8D977C2" modified="2020-10-07" version="1">MSCRYGGGGMVCSLLDLCLNCLRKNISKYGADIKSLPPNIKDKLIKSLSIEGSITDTNISMVLHPAVEALDLRDCDISDYALQQLCNCRHLKKINVNVWKNNRLTITSEGVAALALSCPYLREASFKRCSNVTDSGIHALALNCPLLQIVNIGGCFNITDTALQALGQNCRSLHSVDFSSTQVTDDGVIALVSGMCSKNLKEIHMEHCVNLTDIAVEAVLTYCPMIYILLFHGCPLVTDRSREALEQLVGPKKFKQVSWTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YTV7</accession>
    <name>A0A6I9YTV7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mesoderm induction early response protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">MIER1</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013927475.1">
        <property type="nucleotide sequence ID" value="XM_014072000.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_013927476.1">
        <property type="nucleotide sequence ID" value="XM_014072001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YTV7"/>
    <dbReference type="GeneID" id="106553493"/>
    <dbReference type="KEGG" id="tsr:106553493"/>
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    <dbReference type="OrthoDB" id="5916873at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11661">
        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000025">
        <property type="entry name" value="Mesoderm induction early response 1, transcriptional regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000005">
        <property type="entry name" value="Mesoderm induction early response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040138">
        <property type="entry name" value="MIER/MTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045787">
        <property type="entry name" value="MIER1/3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865:SF24">
        <property type="entry name" value="MESODERM INDUCTION EARLY RESPONSE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865">
        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN AND MESODERM INDUCTION EARLY RESPONSE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19426">
        <property type="entry name" value="MIER1_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ELM2" evidence="9">
        <location>
            <begin position="171"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="274"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="357"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="390"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927476.1"/>
        </source>
    </evidence>
    <sequence length="500" mass="56766" checksum="74924BF2C6617AEF" modified="2020-10-07" version="1">MAEPSVESASPGGSATSDDHEFDPTADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLTREGDMPIHELLSLYGYDGSVPLPEEEEEEEEEEGEGEDNDNNSGCSGENKEETIKDSSGQEDETQSSNGDPVSSVVPEIIRPRRCKYFDTNSEIEEESEEDEDYIPSEDWKKEIMVGSMYQAEIPVGISKYKENEKVYENDDQLLWNPDYLTEDKVIEFLNEASRRTGDERGIDAIPEGSHIKDNEQALYELVKCNFDTDEALRRLRFNVKAAREELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKYNLHPGVTDYMDRLLDESESAASSRAPSPPPTTSNSSTSHSEREDSTTSNSNQNGVSTNGPSELSNKDDVKIEGLHINGPTTGKKPHLVDFDTNGYEPENLSNHSKLPHSNARHDLEDMNERPMKRRRINSSDKECTASSEIFQETVAHGNFEDQDTVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YUT1</accession>
    <name>A0A6I9YUT1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">WNT8A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927400.1">
        <property type="nucleotide sequence ID" value="XM_014071925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YUT1"/>
    <dbReference type="GeneID" id="106553431"/>
    <dbReference type="KEGG" id="tsr:106553431"/>
    <dbReference type="CTD" id="7478"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000003">
        <property type="entry name" value="Protein Wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013301">
        <property type="entry name" value="Wnt8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF92">
        <property type="entry name" value="PROTEIN WNT-8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01892">
        <property type="entry name" value="WNT8PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013927400.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="36724" checksum="7070FCEB16660093" modified="2020-10-07" version="1">MTGPKAYLRYSTSVSAGAQSGIEECKHQFIWERWNCPESALQLSTHHRLRSATRETSFVHAISSAGVMYALTRNCSMGDFENCGCDNSRNGRVGGSGWVWGGCSDNVEFGEKISKHFVDALETAHDARALLNLHNNEAGRLAVKATMKRACKCHGVSGSCSIQTCWLQLADFREVGNYLKNKYNQAQKLEVDKRRIRAGNSAESRGMSTEPFNTIHAADLVFLEDSPDYCIKNMTLGLYGTEGRECLQSGKNLTQWEKRSCKRLCMECGLKIEEKQIEIVTSCNCKFHWCCTVKCEQCKQVVTKYYCSTRVGSFSNNSRQRNRGHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWG2</accession>
    <name>A0A6I9YWG2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch repeat and BTB domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KBTBD3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013928354.1">
        <property type="nucleotide sequence ID" value="XM_014072879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWG2"/>
    <dbReference type="GeneID" id="106554249"/>
    <dbReference type="KEGG" id="tsr:106554249"/>
    <dbReference type="CTD" id="143879"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18480">
        <property type="entry name" value="BACK_KBTBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18271">
        <property type="entry name" value="BTB_POZ_KBTBD3_BKLHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030589">
        <property type="entry name" value="BTB/POZ_KBTBD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047062">
        <property type="entry name" value="KBTBD3_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF418">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="49"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_013928354.1"/>
        </source>
    </evidence>
    <sequence length="613" mass="69922" checksum="D9244BB70C5B1690" modified="2020-10-07" version="1">MENQYNSNCTPTCNGISTHDDKISSLVAENHGQRILTILQSFRAQNLFFDFTILVKDEAIPCHRCVLAACSDFFRAMFEVNMKERDGGSVTISNLTPKAVKAFLDYAYTGKTEITSDNVEMFFQLSSFLQVSLLSKACSDFLIKNIDLVNCLQLLSISESYGSTKLFERTLEYAQLHFSLLLQSNDFLEMNFEILQKCIEADELNVPDEESVLKAVFWWMKHDLETRHKYLPHLMKKIRLHQLPEKTLQDILHSEEQLLKTTNCLAVIKEAIQNVQCFSGLFPDPRPSTTEKYILVHKTGENKVKHTFCYNIKTDQWKELIQINIFDLPGSSLSSYGEKIFITGGCKGNCFRTIRLHIGETFHDATDQTWCYCPANDSFSLVSAMKKPRTMHTSVVILNCLYVIGGKTRASQYIKSLLDVEAYNPLTREWKSMSPLPRGIYYPEASACHSVIYVLGSEIEVADAFNPSLDCFFKYNAVTDQWSELVAEFGQFFHATLIKAIPVNCTLYICDLSTYKVYSFCPETCVWKGEGSFECAGFNAGAVGIQDKIYILGGDYAPDEITDEVQVYHSSRSEWEEVAPMPKALTEFHCQVIQFNKFRDPWCQKQCDTNDYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWG3</accession>
    <name>A0A6I9YWG3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Arachidonate 15-lipoxygenase B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106553882</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013927940.1">
        <property type="nucleotide sequence ID" value="XM_014072465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWG3"/>
    <dbReference type="GeneID" id="106553882"/>
    <dbReference type="KEGG" id="tsr:106553882"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="9">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="10">
        <location>
            <begin position="124"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013927940.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="43082" checksum="B441FF8A5C7BCA2F" modified="2020-10-07" version="1">MAAYKVRVTTGNVCDLGTFDSTSVTLVGTEGESPKTTLGTRSPSFRGDMETEYEITCERPLGEIVLLRLQKEALRDDKSDHWYCDFVTVMSAEEVTYNFPCYQWIEDSWPLELREGTEHVVEYWKRDEFFGYQFLNGLNPTLIRLCAQIPSKFPVTQEMVAASLSDSTTLQEELNKQRIFLMDYHILEGLSPGINNGRLQHIAAPLCLLHLSSEGHLMPLAIQLSQSSSSPIFLPSDPEWDWILAKTWVRNSDFHIHQVLTHILRTHLLAEVFTIATLHQLPMCHPLYKLLIPHTRFTLHINTLARTLLIAKGGVFDKATGTGFEVFMVSMGCSSDNWGWKEFAPGVPYCLDVNSSEELETNVKFSCIKAAIFHENSKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWM4</accession>
    <name>A0A6I9YWM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">WNT5A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928005.1">
        <property type="nucleotide sequence ID" value="XM_014072530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWM4"/>
    <dbReference type="GeneID" id="106553936"/>
    <dbReference type="KEGG" id="tsr:106553936"/>
    <dbReference type="CTD" id="7474"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19347">
        <property type="entry name" value="Wnt_Wnt5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF33">
        <property type="entry name" value="PROTEIN WNT-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 10" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_013928005.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="43047" checksum="1DD5ED90782E49D0" modified="2020-10-07" version="1">MEKSMGTIMQSGALWTGAMTSNYLLLGLTIFSSLLQIMIEGSSWWSLGMNHMNPMNPVQMPEVYIIGAQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNNSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSSCGCSRAARPKDLPRDWLWGGCGDNLDYGYRFAKEFVDARERERIYQKGSYESARILMNIHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMKLNLRGKLVQVNSRFNAPTIYDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YWW8</accession>
    <name>A0A6I9YWW8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytohesin-3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CYTH3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_013928461.1">
        <property type="nucleotide sequence ID" value="XM_014072986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YWW8"/>
    <dbReference type="GeneID" id="106554336"/>
    <dbReference type="KEGG" id="tsr:106554336"/>
    <dbReference type="CTD" id="9265"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01252">
        <property type="entry name" value="PH_GRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000003">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000009">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF320">
        <property type="entry name" value="CYTOHESIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="9">
        <location>
            <begin position="11"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="216"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_013928461.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="40830" checksum="3FE653A0CAE4BE71" modified="2020-10-07" version="1">MTEIDHLTSVEESKATQRNKQVAMGRKKFNMDPKKGIQFLIENDLLQNTPEDIAQFLYKGEGLNKTVIGDYLGERDDFNIKVLQAFVELHEFVDLNLVQALRQFLWSFRLPGEAQKIDRMMETFASRYCLCNPGVFQSTDTCYVLSFAIIMLNTSLHNHNVRDKPTAERFISMNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIKEVEDPRKPNCFELYNPGHKGQVIKACKTEADGRVVEGNHMVYRISAPTPEEKEEWIKSIKASISKDPFYDMLATRKRRIANKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YXV1</accession>
    <name>A0A6I9YXV1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomal protein of 19 kDa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CEP19</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013928771.1">
        <property type="nucleotide sequence ID" value="XM_014073296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YXV1"/>
    <dbReference type="GeneID" id="106554558"/>
    <dbReference type="KEGG" id="tsr:106554558"/>
    <dbReference type="CTD" id="84984"/>
    <dbReference type="OrthoDB" id="2163581at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097712">
        <property type="term" value="P:vesicle targeting, trans-Golgi to periciliary membrane compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029412">
        <property type="entry name" value="CEP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19K CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14933">
        <property type="entry name" value="CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_013928771.1"/>
        </source>
    </evidence>
    <sequence length="163" mass="19399" checksum="FB6BCBB16C2FAE5F" modified="2020-10-07" version="1">MSYVAKKCGIRLQPPSIVLIYEDQLKKNLRQRIMPLRNFTKFSDCSKAAEQLKNNPRHKTYLEGVSLEQLKKLHNLLRCHLQGQTLSQSLKQVEQNETVDPEEDLNKLNDEELAQKKRIMDELFEKNRKRKEDPDFIYNVEVEFPQEKELEACEWDTETEDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZU8</accession>
    <name>A0A6I9YZU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">KIF3A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of KIF3A and KIF3B. Interacts with CIMAP3. Interacts with CLN3. Interacts with DCTN1. Interacts with FLCN. Interacts with AP3B1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929564.1">
        <property type="nucleotide sequence ID" value="XM_014074089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZU8"/>
    <dbReference type="GeneID" id="106555276"/>
    <dbReference type="KEGG" id="tsr:106555276"/>
    <dbReference type="CTD" id="11127"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000028">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF63">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="17"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="375"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="695"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="454"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="703"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="103"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_013929564.1"/>
        </source>
    </evidence>
    <sequence length="730" mass="83039" checksum="F9F348C85B2EF28E" modified="2020-10-07" version="1">MPINRSEKPDKPDNLDNVKVVVRCRPLNEREKTMCYKMAVNVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPESKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAVPELRGIIPNSFAHVFGHIAKAEGDTRFLVRVSYLEIYNEEVRDLLGKDQTQRLEVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSEKGVDGNIHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARINEDPKDALLRQFQKEIEELKKKLEEGEEISGSETSGSEEEEEDEDGEIGEDGEKRKKRRGSSSSSSSDSPCSVIEKPLDKTFTNQAGKKKVSPDKMLEMQAKIDEERKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEKLSALGKKVIVGGVDLLAKAEEQDKLLEESNMELEERRKRAEQLRKELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPQDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVADKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGRSRPKTGRRKRSAKSGAGIDSLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9YZV0</accession>
    <name>A0A6I9YZV0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interferon-inducible GTPase 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106554916</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929140.1">
        <property type="nucleotide sequence ID" value="XM_014073665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9YZV0"/>
    <dbReference type="GeneID" id="106554916"/>
    <dbReference type="KEGG" id="tsr:106554916"/>
    <dbReference type="OrthoDB" id="422720at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF17">
        <property type="entry name" value="IRG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="46"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_013929140.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="46014" checksum="CB6005AA5B2ABC26" modified="2020-10-07" version="1">MAPKAEEDKIALTEKQIQDLKAAFTTGSFDQLTKPLQDAFSSLENISLNVAVVGESGEDRANFINIFRDLCEEDEGAAAPTAKGEARKTPVAYPHPKYPKVILWDLPDIGDPGFSAKRYTEKMDVASYDFFLILASQHFRSFHVNLARYIKKAGKGFYFVRSKVEADLESVRQSNPASFNEMVTLQKIREDCLQDLQAEKVRSPKIFLISSILLSKYDFYLLEEALEKSLDLPKSHSFLLATPNSSHRILEKKKDMMLEHLWLVAVVACGIQTDAIPDISVVCNVDLLVRTLRGYCISFGLEEESLRRTAEHVVQPIEQLRALVRSPLAAAVSKGLVVDLLIQAASRAPNLPKQLVPMASVGLSFAVVYSMLKAFVEDVATDAHRLLVKVFMSHKGTSEPETSVSKALENQGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z016</accession>
    <name>A0A6I9Z016_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">OSBPL2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013929902.1">
        <property type="nucleotide sequence ID" value="XM_014074427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z016"/>
    <dbReference type="GeneID" id="106555554"/>
    <dbReference type="KEGG" id="tsr:106555554"/>
    <dbReference type="CTD" id="9885"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000005">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000003">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF153">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="316"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_013929902.1"/>
        </source>
    </evidence>
    <sequence length="474" mass="54766" checksum="62385EBA5135C08A" modified="2020-10-07" version="1">MNSEEEFYDAVTGFDSDNSSGEFSEAVHKVDVDDHQSNRTGKHNGDGPVQENGIKKHRTSLPAPMFTRSDFSVWSILKKCIGLELSKITMPIAFNEPLSFLQRITEYMEHTYLINKANCHSNPLERMQIVAAFAVSAVASQWERTGKPFNPLLGETYELIREDLGFRLISEQVSHHPPISAFYSEGLNQDFIFHGSIYPKLKFWGKSVEAEPRGTITLELIKHHEAFTWTNPTCCVHNVIIGKLWIEQYGTVEIINHSTGDKCILNFKPCGLFGKELHRIEGYIQDKNKKKLSVIYGKWTECLWCVDPYSYENYKKNERKGGDQKKPKPRDEPENDEADDMPDVQETVQVIPGSKLLWRISSRPSNSSQMYNFTSFTVTLNELEKGMEDILAPTDCRLRPDIRHMENGEIDMASNEKERLEEKQRTARKDRAKEEIDWRTRWFYQGTNPYTGTADWLYSGGYFDRNFFDCPDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z2K0</accession>
    <name>A0A6I9Z2K0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytochrome c oxidase subunit NDUFA4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NDUFA4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Skeletal muscle</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_013930095.1">
        <property type="nucleotide sequence ID" value="XM_014074620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z2K0"/>
    <dbReference type="GeneID" id="106555723"/>
    <dbReference type="KEGG" id="tsr:106555723"/>
    <dbReference type="CTD" id="4697"/>
    <dbReference type="OrthoDB" id="5511684at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256:SF1">
        <property type="entry name" value="GEO09626P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_013930095.1"/>
        </source>
    </evidence>
    <sequence length="105" mass="11927" checksum="21903EE48A2D55E1" modified="2020-10-07" version="1">MSQQQQPQQPRAPPAASAASNPGLIGFLRHQVTHRPSLIPLFLIMGTAVAGAGLYLMRLALLCPEVRWDKKNNPEPWNNLDPTHRYKFFAVNMDYSKLQKDRPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z307</accession>
    <name>A0A6I9Z307_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum membrane sensor NFE2L1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Nuclear factor erythroid 2-related factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Nuclear factor, erythroid derived 2, like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC106555948</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Skeletal muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bZIP family. CNC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930366.1">
        <property type="nucleotide sequence ID" value="XM_014074891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z307"/>
    <dbReference type="GeneID" id="106555948"/>
    <dbReference type="OrthoDB" id="7458135at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14720">
        <property type="entry name" value="bZIP_NFE2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.880.10:FF:000001">
        <property type="entry name" value="Nuclear factor erythroid 2-related factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.880.10">
        <property type="entry name" value="Transcription factor, Skn-1-like, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047167">
        <property type="entry name" value="NFE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF31">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE SENSOR NFE2L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411">
        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="21" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BZIP" evidence="27">
        <location>
            <begin position="317"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="153"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="247"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="156"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_013930366.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="48766" checksum="E3C3EB3858F3B737" modified="2020-10-07" version="1">MSIMETQSMEVNSTTLYNSSSGNLLTANYSLAPSTPINQNVSLHQASLVGCTQDFPSLFNSESESPSVAGGSALLQLVPDNSTGFNKFGSTNLSGNFFPPQLNCTVTETSGSELPDPLGGLLDEAMLDEISLMDLAIEEGFNPVQASCLEEEFDSDSGLSLDSSHSPASLSSSEASSSSSFSEEGAVGYSSDTENVDFEDAEGAVGYQPEYSKFYRMSYQNPPQLQYLPYHEHVGHNQTYNMAPRTLDTDESQPASMGKKSSKEKQADFLDKQMSRDEHRARAMKIPFPNEKIINLPVEEFNELLSKYQLSEAQLSLIRDIRRRGKNKMAAQNCRKRKLDTILNLERDVDDLQRDKSKLLREKVEFLRSIHQMKQKMQTLYQEVFGHLRDENGQPYSPNQYILQHASDGSVILISRSLAEQQRWCQEKKQKDRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3P6</accession>
    <name>A0A6I9Z3P6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FERM domain-containing protein 4A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106556140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_013930591.1">
        <property type="nucleotide sequence ID" value="XM_014075116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3P6"/>
    <dbReference type="GeneID" id="106556140"/>
    <dbReference type="KEGG" id="tsr:106556140"/>
    <dbReference type="OrthoDB" id="10063592at2759"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090162">
        <property type="term" value="P:establishment of epithelial cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047176">
        <property type="entry name" value="FRMD4A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46079">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46079:SF3">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_013930591.1"/>
        </source>
    </evidence>
    <sequence length="447" mass="49519" checksum="48C0E65C59E4CEEE" modified="2020-10-07" version="1">MHYHRNDYDKSPIKPKMWTESSLDEPYEKVKKRSSHSHSSHKRFPSTGSCVEGGGSNSLQNSPVRNLPHWNTQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHCLVQHFRHRSSSLESQGKLLGSENETGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPAQMNANYSTLAEDSPSKARERQRQRHKSAGNLVCSNSGSMPNLAAGNGNGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSTPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMFKENWHGDEVDSIRLTPSRSQIIRTPSLGRDGHEKGSGRAGVSDELRLWYQRSTAPHKDHSRLSHTSSTSSDSSSQYSSSSQSTFVAHSKVTRMPPMCKATSAALPHHPRTSTTTPSDCGSLTPSCPHHILAWQTGSYSESCYLDSALYPELSDVQWYGQDKAKPGTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I9Z3V3</accession>
    <name>A0A6I9Z3V3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cullin-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CUL5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thamnophis sirtalis</name>
        <dbReference type="NCBI Taxonomy" id="35019"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Lepidosauria</taxon>
            <taxon>Squamata</taxon>
            <taxon>Bifurcata</taxon>
            <taxon>Unidentata</taxon>
            <taxon>Episquamata</taxon>
            <taxon>Toxicofera</taxon>
            <taxon>Serpentes</taxon>
            <taxon>Colubroidea</taxon>
            <taxon>Colubridae</taxon>
            <taxon>Natricinae</taxon>
            <taxon>Thamnophis</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 11">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_013930646.1">
        <property type="nucleotide sequence ID" value="XM_014075171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I9Z3V3"/>
    <dbReference type="GeneID" id="106556184"/>
    <dbReference type="KEGG" id="tsr:106556184"/>
    <dbReference type="CTD" id="8065"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000504617">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000142">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000009">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000014">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000017">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000004">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="12">
        <location>
            <begin position="382"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000504617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_013930646.1"/>
        </source>
    </evidence>
    <seque
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